The functional hereditary units of FUNGI.
Deoxyribonucleic acid that makes up the genetic material of fungi.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans and in some other male-heterogametic species in which the homologue of the X chromosome has been retained.
The teaching staff and members of the administrative staff having academic rank in a medical school.
The human male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans.
The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Geological formations consisting of underground enclosures with access from the surface.
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Any method used for determining the location of and relative distances between genes on a chromosome.
Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".
A United States organization of distinguished scientists and engineers established for the purpose of investigating and reporting upon any subject of art or science as requested by any department of government. The National Research Council organized by NAS serves as the principal operating agency to stimulate and support research.
VERTEBRATES belonging to the class amphibia such as frogs, toads, newts and salamanders that live in a semiaquatic environment.
The study of natural phenomena by observation, measurement, and experimentation.
An order of the class Amphibia, which includes several families of frogs and toads. They are characterized by well developed hind limbs adapted for jumping, fused head and trunk and webbed toes. The term "toad" is ambiguous and is properly applied only to the family Bufonidae.
Surgical fistulization of the LACRIMAL SAC for external drainage of an obstructed nasolacrimal duct.
Partial or total replacement of all layers of a central portion of the cornea.
A tubular duct that conveys TEARS from the LACRIMAL GLAND to the nose.
Interference with the secretion of tears by the lacrimal glands. Obstruction of the LACRIMAL SAC or NASOLACRIMAL DUCT causing acute or chronic inflammation of the lacrimal sac (DACRYOCYSTITIS). It is caused also in infants by failure of the nasolacrimal duct to open into the inferior meatus and occurs about the third week of life. In adults occlusion may occur spontaneously or after injury or nasal disease. (Newell, Ophthalmology: Principles and Concepts, 7th ed, p250)
Diseases of the lacrimal apparatus.
Inflammation of the lacrimal sac. (Dorland, 27th ed)
An abnormal concretion occurring mostly in the urinary and biliary tracts, usually composed of mineral salts. Also called stones.
A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)
Proteins found in any species of fungus.
A family of fused-ring hydrocarbons isolated from coal tar that act as intermediates in various chemical reactions and are used in the production of coumarone-indene resins.
Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.
A watery fluid that is continuously produced in the CHOROID PLEXUS and circulates around the surface of the BRAIN; SPINAL CORD; and in the CEREBRAL VENTRICLES.
Microtubule-associated proteins that are mainly expressed in neurons. Tau proteins constitute several isoforms and play an important role in the assembly of tubulin monomers into microtubules and in maintaining the cytoskeleton and axonal transport. Aggregation of specific sets of tau proteins in filamentous inclusions is the common feature of intraneuronal and glial fibrillar lesions (NEUROFIBRILLARY TANGLES; NEUROPIL THREADS) in numerous neurodegenerative disorders (ALZHEIMER DISEASE; TAUOPATHIES).
A single-pass type I membrane protein. It is cleaved by AMYLOID PRECURSOR PROTEIN SECRETASES to produce peptides of varying amino acid lengths. A 39-42 amino acid peptide, AMYLOID BETA-PEPTIDES is a principal component of the extracellular amyloid in SENILE PLAQUES.
A parliamentary democracy with a constitutional monarch in southeast Asia, consisting of 11 states (West Malaysia) on the Malay Peninsula and two states (East Malaysia) on the island of BORNEO. It is also called the Federation of Malaysia. Its capital is Kuala Lumpur. Before 1963 it was the Union of Malaya. It reorganized in 1948 as the Federation of Malaya, becoming independent from British Malaya in 1957 and becoming Malaysia in 1963 as a federation of Malaya, Sabah, Sarawak, and Singapore (which seceded in 1965). The form Malay- probably derives from the Tamil malay, mountain, with reference to its geography. (From Webster's New Geographical Dictionary, 1988, p715 & Room, Brewer's Dictionary of Names, 1992, p329)
Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Any arthropod of the subclass ACARI except the TICKS. They are minute animals related to the spiders, usually having transparent or semitransparent bodies. They may be parasitic on humans and domestic animals, producing various irritations of the skin (MITE INFESTATIONS). Many mite species are important to human and veterinary medicine as both parasite and vector. Mites also infest plants.
Antigens from the house dust mites (DERMATOPHAGOIDES), mainly D. farinae and D. pteronyssinus. They are proteins, found in mite feces or mite extracts, that can cause ASTHMA and other allergic diseases such as perennial rhinitis (RHINITIS, ALLERGIC, PERENNIAL) and atopic dermatitis (DERMATITIS, ATOPIC). More than 11 groups of Dermatophagoides ALLERGENS have been defined. Group I allergens, such as Der f I and Der p I from the above two species, are among the strongest mite immunogens in humans.
Leisure activities engaged in for pleasure.
Insects of the order Dictyoptera comprising several families including Blaberidae, BLATTELLIDAE, Blattidae (containing the American cockroach PERIPLANETA americana), Cryptocercidae, and Polyphagidae.
Earth or other matter in fine, dry particles. (Random House Unabridged Dictionary, 2d ed)
Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.
A species of imperfect fungi from which the antibiotic nidulin is obtained. Its teleomorph is Emericella nidulans.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A normally saprophytic mitosporic Chaetothyriales fungal genus. Infections in humans include PHAEOHYPHOMYCOSIS; and PERITONITIS.. Exophiala jeanselmei (previously Phialophora jeanselmei) is an etiological agent of MYCETOMA.
Microscopic threadlike filaments in FUNGI that are filled with a layer of protoplasm. Collectively, the hyphae make up the MYCELIUM.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae. (1/8779)

Replication fidelity is controlled by DNA polymerase proofreading and postreplication mismatch repair. We have genetically characterized the roles of the 5'-->3' Exo1 and the 3'-->5' DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using various genetic backgrounds and highly sensitive mutation detection systems that are based on long and short homonucleotide runs. Genetic interactions were examined among DNA polymerase epsilon (pol2-4) and delta (pol3-01) mutants defective in 3'-->5' proofreading exonuclease, mutants defective in the 5'-->3' exonuclease Exo1, and mismatch repair mutants (msh2, msh3, or msh6). These three exonucleases play an important role in mutation avoidance. Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes.  (+info)

Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. (2/8779)

Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex.  (+info)

Histone octamer transfer by a chromatin-remodeling complex. (3/8779)

RSC, an abundant, essential chromatin-remodeling complex related to SWI/SNF complex, catalyzes the transfer of a histone octamer from a nucleosome core particle to naked DNA. The newly formed octamer-DNA complex is identical with a nucleosome in all respects. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. The mechanism may entail formation of a duplex displacement loop on the nucleosome, facilitating the entry of exogeneous DNA and the release of the endogenous molecule.  (+info)

Regulation of the start of DNA replication in Schizosaccharomyces pombe. (4/8779)

Cells of Schizosaccharomyces pombe were grown in minimal medium with different nitrogen sources under steady-state conditions, with doubling times ranging from 2.5 to 14 hours. Flow cytometry and fluorescence microscopy confirmed earlier findings that at rapid growth rates, the G1 phase was short and cell separation occurred at the end of S phase. For some nitrogen sources, the growth rate was greatly decreased, the G1 phase occupied 30-50% of the cell cycle, and cell separation occurred in early G1. In contrast, other nitrogen sources supported low growth rates without any significant increase in G1 duration. The method described allows manipulation of the length of G1 and the relative cell cycle position of S phase in wild-type cells. Cell mass was measured by flow cytometry as scattered light and as protein-associated fluorescence. The extensions of G1 were not related to cell mass at entry into S phase. Our data do not support the hypothesis that the cells must reach a certain fixed, critical mass before entry into S. We suggest that cell mass at the G1/S transition point is variable and determined by a set of molecular parameters. In the present experiments, these parameters were influenced by the different nitrogen sources in a way that was independent of the actual growth rate.  (+info)

The prokaryotic beta-recombinase catalyzes site-specific recombination in mammalian cells. (5/8779)

The development of new strategies for the in vivo modification of eukaryotic genomes has become an important objective of current research. Site-specific recombination has proven useful, as it allows controlled manipulation of murine, plant, and yeast genomes. Here we provide the first evidence that the prokaryotic site-specific recombinase (beta-recombinase), which catalyzes only intramolecular recombination, is active in eukaryotic environments. beta-Recombinase, encoded by the beta gene of the Gram-positive broad host range plasmid pSM19035, has been functionally expressed in eukaryotic cell lines, demonstrating high avidity for the nuclear compartment and forming a clear speckled pattern when assayed by indirect immunofluorescence. In simian COS-1 cells, transient beta-recombinase expression promoted deletion of a DNA fragment lying between two directly oriented specific recognition/crossing over sequences (six sites) located as an extrachromosomal DNA substrate. The same result was obtained in a recombination-dependent lacZ activation system tested in a cell line that stably expresses the beta-recombinase protein. In stable NIH/3T3 clones bearing different number of copies of the target sequences integrated at distinct chromosomal locations, transient beta-recombinase expression also promoted deletion of the intervening DNA, independently of the insertion position of the target sequences. The utility of this new recombination tool for the manipulation of eukaryotic genomes, used either alone or in combination with the other recombination systems currently in use, is discussed.  (+info)

The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance. (6/8779)

The mdm17 mutation causes temperature-dependent defects in mitochondrial inheritance, mitochondrial morphology, and the maintenance of mitochondrial DNA in the yeast Saccharomyces cerevisiae. Defects in mitochondrial transmission to daughter buds and changes in mitochondrial morphology were apparent within 30 min after shifting cells to 37 degrees C, while loss of the mitochondrial genome occurred after 4-24 h at the elevated temperature. The mdm17 lesion mapped to MGM1, a gene encoding a dynamin-like GTPase previously implicated in mitochondrial genome maintenance, and the cloned MGM1 gene complements all of the mdm17 mutant phenotypes. Cells with an mgm1-null mutation displayed aberrant mitochondrial inheritance and morphology. A version of mgm1 mutated in a conserved residue in the putative GTP-binding site was unable to complement any of the mutant defects. It also caused aberrant mitochondrial distribution and morphology when expressed at high levels in cells that also contained a wild-type copy of the gene. Mgm1p was localized to the mitochondrial outer membrane and fractionated as a component of a high molecular weight complex. These results indicate that Mgm1p is a mitochondrial inheritance and morphology component that functions on the mitochondrial surface.  (+info)

All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature. (7/8779)

All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned. These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature. The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs. Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex.  (+info)

The nuclear ribosomal DNA intergenic spacer as a target sequence to study intraspecific diversity of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum directly on pinus root systems. (8/8779)

Polymorphism of the nuclear ribosomal DNA intergenic spacer (IGS) of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum was studied to evaluate whether this sequence could be used in field studies to estimate the diversity of strains forming mycorrhizas on individual Pinus pinaster root systems. This sequence was amplified by PCR from 125 haploid homokaryotic strains collected in 14 P. pinaster stands along the Atlantic coast of France by using conserved oligonucleotide primers. Restriction enzyme digestion of the amplified 3.4-kbp-long IGS allowed us to characterize 24 alleles whose frequencies differed. Nine of these alleles were found only once, whereas about 60% of the strains contained four of the alleles. Local populations could be almost as diverse as the entire population along a 150-km stretch of coastline that was examined; for example, 13 alleles were found in a single forest stand. The IGS from one strain was partially sequenced, and the sequence data were used to design oligonucleotides which allowed separate PCR amplification of three different segments of the IGS. Most polymorphisms observed among the full-length IGS regions resulted from polymorphisms in an internal ca. 1,500-bp-long sequence characterized by length variations that may have resulted from variable numbers of a T2AG3 motif. This internal polymorphic sequence could not be amplified from the genomes of nine other Hebeloma species. Analysis of this internal sequence amplified from the haploid progenies of 10 fruiting bodies collected in a 70-m2 area resulted in identification of six allelic forms and seven distinct diplotypes out of the 21 possible different combinations. Moreover, optimization of the PCR conditions resulted in amplification of this sequence from more than 80% of the DNA samples extracted from individual H. cylindrosporum infected P. pinaster mycorrhizal root tips, thus demonstrating the usefulness of this sequence for studying the below-ground diversity of mycorrhizas formed by genets belonging to the same fungal species.  (+info)

Centromere DNA element II (CDEII) of budding yeast centromeres is an AT-rich sequence essential for centromere (CEN) function. Sequence analysis of Saccharomyces cerevisiae CDEIIs revealed that A(5-7)/T(5-7) tracts are statistically overrepresented at the expense of AA/TT and alternating AT. To test the hypothesis that this nonrandom sequence organization is functionally important, a CEN library in which the CDEII sequences were randomized was generated. The library was screened for functional and nonfunctional members following centromere replacement in vivo. Functional CENs contained CDEIIs with the highly biased A(n)/T(n) run distribution of native centromeres, while nonfunctional CDEIIs resembled those picked from the library at random. Run content, defined as the fraction of residues present in runs of four or more nucleotides, of the functional and nonfunctional CDEII populations differed significantly (P | 0.001). Computer searches of the genome for regions with an A + T content comparable to
OUTPUTS: Fungal DNA has been selectively PCR amplified and sequenced from 45 healthy humans on conventional Western diets: from two time points for 24 individuals and from one time point for 21 individuals. Multiple unsuccessful efforts were made to amplify fungal DNA from the second time point for the 21 individuals; these DNA samples have successfully yielded bacterial DNA amplification and sequence, and we thus conclude fungal DNA to be below the level of detection in these samples. A manuscript discussing results is in preparation and will be submitted by the end of 2012. DNA has been extracted from fecal samples from 20 healthy humans on non-conventional diets (vegetarian, vegan, gluten-free, lactose-free) and 2 infants. Selective PCR amplification of fungal DNA from these samples is ongoing and will be followed by DNA pyrosequencing in late 2012 and early 2013. Preliminary data on dominant fungi has been obtained by selective culturing and/or quantitative PCR, and results will be presented ...
The new assemblies also had many fewer gaps and greatly improved continuity compared to the originally published versions. For instance, our unordered S. mikatae assembly consisted of 1220 scaffolds, of which 159 were longer than 500 bp, and many of the shorter ones were likely to be spurious byproducts of the short-read assembly process. Notably, those 159 scaffolds accounted for ,98% of the assembly and contained just 18 gaps (Table 2). By contrast, the previous assemblies consisted of more than 300 scaffolds longer than 500 bp, and these contained ,1300 gaps averaging ,600 bp. Indeed, the contig N50s were on the order of 20 kb compared to our scaffold N50 of ,360 kb (a fair comparison given the paucity of gaps in our assembly). Though the relative improvement varied by species, the N50 range (151-445 kb) of our unordered assemblies represented significant improvements in all cases (Table 2).. Excluding sub-telomeric regions, five S. bayanus chromosomes (I, VI, XI, XII, XIII) were each ...
Promoters play a central role in transcription initiation and gene regulation, so it is necessary for these DNA regions to be differentiated from non-promoter sequences. Sequence motif based computational methods have not been able to identify these regio
Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains.
Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains.
Hello, I was wondering if anyone can recommend good study material for the CEN exam. I understand that the exam has a high first-time fail rate. I want to pass the first time! Thanks, David
Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(11)2-Cys6 binuclear cluster domin and a putative alpha2-SCB-alpha2 binding site ...
In a previous attempt to identify as many as possible of the essential genes on Saccharomyces cerevisiae chromosome I, temperature-sensitive (Ts-) lethal mutations that had been induced by ethyl methane-sulfonate or nitrosoguanidine were analyzed. Thirty-two independently isolated mutations that mapped to chromosome I identified only three complementation groups, all of which had been known previously. In contrast, molecular analyses of segments of the chromosome have suggested the presence of numerous additional essential genes. In order to assess the degree to which problems of mutagen specificity had limited the set of genes detected using Ts- lethal mutations, we isolated a new set of such mutations after mutagenesis with UV or nitrogen mustard. Surprisingly, of 21 independently isolated mutations that mapped to chromosome I, 17 were again in the same three complementation groups as identified previously, and two of the remaining four mutations were apparently in a known gene involved in ...
ID YEP367 preliminary; circular DNA; SYN; 8400 BP. XX AC ATCC37735; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE Saccharomyces/E.coli plasmid vector YEp367 - incomplete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC YEp352E from YEp352 & linker RC YEp363A from pNM480 & YEp351 RC YEp353A from pNM480 & YEp352 RC YEp353 from YEp353A & YEp352E RC YEp354A from pNM481 & YEp352 RC YEp354 from YEp354A & YEp352E RC YEp355A from pNM482 & YEp352 RC YEp355 from YEp355A & YEp352E RC YEp356, YEp356R from YEp353 & pUC18 RC YEp357, YEp357R from YEp354 & pUC18 RC YEp358, YEp358R from YEp355 & pUC18 RC YEp363 from YEp363A & YEp353 RC YEp364 from YEp363A & YEp354 RC YEp365 from YEp363A & YEp355 RC YEp366 from YEp363A & YEp356 RC YEp367 from YEp363A & YEp357 RC YEp368 from YEp363A & YEp358 RC YEp366R from YEp363A & YEp356R RC YEp367R from YEp363A & YEp357R RC YEp368R from YEp363A & YEp358R RC YIp353 from YEp353 & ...
ID YEP353 preliminary; circular DNA; SYN; 7944 BP. XX AC U03500; ATCC37725; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE Saccharomyces/E.coli plasmid vector YEp353 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RP 1-7944 RC YEp352E from YEp352 & linker RC YEp363A from pNM480 & YEp351 RC YEp353A from pNM480 & YEp352 RC YEp353 from YEp353A & YEp352E RC YEp354A from pNM481 & YEp352 RC YEp354 from YEp354A & YEp352E RC YEp355A from pNM482 & YEp352 RC YEp355 from YEp355A & YEp352E RC YEp356, YEp356R from YEp353 & pUC18 RC YEp357, YEp357R from YEp354 & pUC18 RC YEp358, YEp358R from YEp355 & pUC18 RC YEp363 from YEp363A & YEp353 RC YEp364 from YEp363A & YEp354 RC YEp365 from YEp363A & YEp355 RC YEp366 from YEp363A & YEp356 RC YEp367 from YEp363A & YEp357 RC YEp368 from YEp363A & YEp358 RC YEp366R from YEp363A & YEp356R RC YEp367R from YEp363A & YEp357R RC YEp368R from YEp363A & YEp358R RC YIp353 ...
Alignment of the centromere regions of all sixteen chromosomes. The regions include the Centromere DNA Elements I II and III (CDEI, CDEII and CDEIII). The conserved bases in all centromeres are marked in magenta. The regions with less conserved residues of CDEI and CDEIII are marked in green. The CDEII region which contains more than 90% AT residues has been left white. The multiple sequence alignment was created with PILEUP ...
Calculate the bounded phylogenetic biodiversity metrics: phylogenetic species variability, richness, evenness and clustering for one or multiple samples.
PCR/RFLP of the NTS2 sequence of rDNA was shown to be suitable for differentiating Saccharomyces sensu stricto species. We previously showed that, within the presently accepted S. bayanus taxon, strains formerly classified as S. uvarum represented a distinct subgroup (Nguyen and Gaillardin, 1997). I …
Ability, Body Fluids, Cerebrospinal Fluid, Cladosporium, Culture, Diagnostic Test, DNA, Fungal DNA, Health, Infection, Infections, Injections, Methylprednisolone, Patient, Patients, PCR, Persons, Public Health, Report, Role
For the most part, the Agaricales are fleshy fungi that are supple at maturity and last for no more than a week or two in nature. The Boletales are also fleshy and can be confused with other fungi that have pores and are hard. Some of these hard fungi are common parasites of trees; the shelves that they produce can grow for years ...
At this time, fungal populations in newborns and their long-term impact on health have been very little studied. In babies aged 0 to 1 month, their characterization shows low diversity and relative stability but site-dependent variations, linked in part to the mode of delivery. Th ...
Seven isolates of Candida stellatoidea were studied for their electrophoretic karyotype, virulence for mice, sensitivity to UV radiation, growth rate in vitro, reaction on cycloheximide-indicator medium, and proteinase activity. The isolates exhibited one of two distinct electrophoretic karyotypes as determined by orthogonal field alternating gel electrophoresis (OFAGE). Four isolates, including the type culture of C. stellatoidea, belonged to electrophoretic karyotype type I by OFAGE, showing eight to nine bands of which at least two bands were less than 1,000 kilobases in size as estimated by comparison with the DNA bands of Saccharomyces cerevisiae. These isolates failed to produce fatal infection in mice within 20 days when 5 X 10(5) cells were injected intravenously. The yeasts were cleared from the kidneys of two of three mice tested by day 30. Type I showed proteinase activity on bovine serum albumin agar at pH 3.8 and produced a negative reaction on cycloheximide-bromcresol green medium ...
The phylogenetic species concept defines a species as a group of organisms that shares a common ancestor and can be distinguished from other organisms that do not share that ancestor. As an analogy,...
Frøslev, Tobias Guldberg & Thomas Stjernegaard Jeppesen. Three new species of Cortinarius subgenus Phlegmacium. Mycotaxon 106: 469-477. 2008. ABSTRACT: Based on morphological and molecular phylogenetic studies we present three new species in Cortinarius. Species descriptions are provided, along with discussions of phylogenetic and morphological affinities to similar taxa. Cortinarius majoranae, Cortinarius aquilanus and Cortinarius lepistoides spp. nov. are described. The first taxon is placed in section Percomes, whereas the last two belong to section Caerulescentes. KEYWORDS: taxonomy, phylogenetic relationships, European distribution ...
Infections and excesses of yeasts are common in humans in the West. Some 70 per cent of human have excesses of yeasts at some point in their lives. Excesses are caused when the commensal bacteria of the gut (the good guys) are compromised and fail to deal with the yeasts. At night in a healthy individual, 2.2 pounds of yeasts are consumed by commensal bacteria. Without their presence, the yeasts can flourish. They can punch holes in the gut wall (Leaky Gut Syndrome) allowing food molecules into the blood stream, resulting in fatigue. In worse cases the yeasts can get into the blood stream, blocking cell receptor sites and colonising certain areas of the body reducing localised oxygen levels ...
Cortinarius alboviolaceus silkkiseitikki valkjas v dik blekviolett spindling lil sfeh r p kh l sgomba Pearly Webcap lysviolet slørhat lysfiolett slørsopp lila gordijnzwam ...
Browse diverse articles and stories on fermentis yeast strains. The information of fermentis yeast strains is constantly complemented and updated on echemi.com.
Vaccination using a fungal strain protein can protect against the infections caused by drug-resistant fungal species . beat campus Education Times
DNA is the hereditary material that contains the genetic code that makes up an organism. Explore more about DNA, its structure and functions at BYJUS.
Hamdy4「緩解能藉由維持良好的飲食與規律的運動而延長》「. 患有二型糖尿病的人只要吃得正確3並持續保持身材就能讓此疾病保持在不需要藥物或是胰島素幫助治療的狀態》只是擁有計畫跟確實執行是完全不同的兩件事情》. Parrish4「我寫下每一個單一食物的熱量與碳水化合物的重量3並在一天的最後將它們加總起來》一旦我停下來作這件事情3我的體重就又開始增加》「. Hamdy 說3幹勁是能否長期成功的關鍵3而維持幹勁最好的方法就是設定可達到的目標》. 「當病患看到接近目標的成效便會被激勵》而當他們看到改善3他們甚至會變得更有幹勁》「. 以上是 Parrish ...
研究證實聽音樂或處在歡笑的情境下可能是另一種降低高血壓的方法》. 新研究指出中年男女或許能藉由多笑與聽他們喜歡的音樂來減少血壓計的讀數》 日本大阪大學的研究者們為了確定音樂與笑聲能否降低血壓3因此安排了兩種情況41. 聽到音樂或笑聲後立即測量》2. 在三個月期間內每週一次3讓參與者聽音樂或讓他們歡笑一個小時3並在結束後測量血壓》 科學家讓 79 位年齡在 40 ~ 74 歲的參與者隨機分成三組》32 位聽音樂 30 位是笑聲組3而最後的 17 位既不聽音樂也沒被安排到歡笑的情境》 在每種音樂或是歡笑情境的前後均會測量血壓》 ...
In budding yeast replication origins, the 11-bp ARS consensus sequence is essential for interaction with the ORC. However, replication origins in other eukaryotic species, including fission yeast, do not appear to contain a short essential sequence (15,23) and it has not been known whether the ORC is located at chromosomal replication origins. The present study demonstrated that a fission yeast ORC subunit and an Mcm protein are specifically localized at chromosomal replication origins. Orp1p is located at thears2004 and ars3002 loci throughout the cell cycle, while SpMcm6p is associated with these origins only in the G1 and S phases. To our knowledge, this is the first indication of preferential localization of the ORC and Mcm proteins at the chromosomal replication origins in eukaryotic species except for budding yeast.. The CHIP assay finding that Orp1p was localized at ars2004and ars3002 but not at non-ARS regions (Fig. 6) suggests that a certain sequence or DNA structure in the replication ...
Maize infected by aflatoxin-producing Aspergillus flavus may become contaminated with aflatoxins, and as a result, threaten human health, food security and farmers income in developing countries where maize is a staple. Environmental distribution and genetic diversity of A. flavus can influence the effectiveness of atoxigenic isolates in mitigating aflatoxin contamination. However, such information has not been used to facilitate selection and deployment of atoxigenic isolates. A total of 35 isolates of A. flavus isolated from maize samples collected from three agroecological zones of Nigeria were used in this study. Ecophysiological characteristics, distribution and genetic diversity of the isolates were determined to identify vegetative compatibility groups (VCGs). The generated data were used to inform selection and deployment of native atoxigenic isolates to mitigate aflatoxin contamination in maize. In co-inoculation with toxigenic isolates, atoxigenic isolates reduced aflatoxin ...
Amplified fragment length polymorphism (AFLP) genotyping of isolates of the pathogenic fungus Cryptococcus neoformans suggested a considerable genetic divergence between the varieties C. neoformans var. neoformans and C. neoformans var. grubii on the one hand versus C. neoformans var. gattii on the other. This divergence is supported by additional phenotypic, biochemical, clinical and molecular differences. Therefore, the authors propose the existence of two species, C. neoformans (Sanfelice) Vuillemin and C. bacillisporus Kwon-Chung, which differ in geographical distribution, serotypes and ecological origin. Within each species three AFLP genotypes occur, which differ in geographical distribution and serotypes. Differences in ecological origin (AIDS patients, non-AIDS patients, animals or the environment) were found to be statistically not significant. In C. neoformans as well as in C. bacillisporus one of the genotypes represented a hybrid. The occurrence of hybridization has consequences for the
SCDNAALG2 X87947 3123bp DNA PLN 16-JUN-1995 S.cerevisiae ALG2 gene. ALG2 gene; glycosyltransferase; ALG2. SCJ1PROM Z49780 573bp DNA PLN 13-JUN-1995 S.cerevisiae promoter DNA (573 bp). SCVRP1GEN X87806 3423bp DNA PLN 13-JUN-1995 S.cerevisiae VRP1 gene. verprolin; vrp1 gene; vrp1. YSCF4121 D44598 18837bp DNA PLN 24-JUN-1995 Saccharomyces cerevisiae chromosome VI phage 4121. DNA-directed RNA polymerase mitochondrial; GTP-binding protein YPT1; actin; tubulin beta chain; ACT1; ACTIN; YPT1; GTP-BINDING PROTEIN YPT1(YP2); TUB2; TUBULIN BETA CHAIN; RPO41; DNA-DIRECTED RNA POLYMERASE MITOCHONDRIAL. YSCF9965 D44597 36230bp DNA PLN 28-JUN-1995 Saccharomyces cerevisiae chromosome VI cosmid 9965. hexokinase A; mitochondrial ribosomal protein; nuclearintegrity protein 1; proteosome component PRE4; YMR31; PRE4; NIN1; nuclearintegrity protein 1; HXK1; HEXOKINASE A. YSCF9993 D44603 35881bp DNA PLN 24-JUN-1995 Saccharomyces cerevisiae chromosome VI cosmid 9993. To obtain any of the yeast GenBank sequences you can ...
We have analyzed the karyotype of the rice blast fungus, Magnaporthe grisea, by using pulsed-filed gel electrophoresis. We tested whether the electrophoretic karyotype of an isolate was related to its pathotype, as determined by infection assays, or its genetic lineage, as determined by DNA fingerprinting. Highly reproducible electrophoretic karyotypes were obtained for a collection of U.S. and Chinese isolates representing a diverse collection of pathotypes and genetic lineages. Chromosomes ranged in size from 3 to 10 Mb. Although chromosome number was largely invariant, chromosome length polymorphisms were frequent. Minichromosomes were also found, although their presence was not ubiquitous. They ranged in number from 1 to 3 and in size from 470 kb to 2.2 Mb. Karyotypes were sufficiently variable as to obscure the obvious relatedness of isolates on the basis of pathogenicity assays or genetic lineage analysis by DNA fingerprinting. We documented that the electrophoretic karyotype of an isolate ...
chapter 1) Comparative organization around the MAT locus in the Ascomycota. The main horizontal line shows the organization of the MAT locus in homothallic species, or the idiomorph (where known) in heterothallic species. The organization of the a idiomorph is represented by the offset box below the idiomorph. The nomenclature suggested in reference 67 is used for the Pezizomycotina (e.g., 1-1-1 represents MAT1-1-1). Orthologous genes are connected by gray lines. Conserved groups of genes are indicated by color: red, idiomorph; green, a idiomorph; yellow, APN2; purple, SLA2 and homologs of S. cerevisiae XIV; orange, homologs of S. cerevisiae chromosome X; blue, homologs of S. cerevisiae chromosome III (YCR033W-YCR038W); white, homologs of S. cerevisiae chromosome III (YCR042C-YCR045C); gray, homologs of S. cerevisiae chromosome XI (YLR186W-YLR182W); gray gradient, CAN1 (YEL063C). The position of an Ho endonuclease site in MATa1 is marked where present. The figure was redrawn from Butler et al. ...
The means by which humans acquire Pneumocystis carinii is not well understood. Whether it can be acquired from specific environmental sources or transmitted from person to person has not been determined. This study was designed to detect nucleic acids of P. carinii in air samples from various locations, including P. carinii-infected patients...
An overview of the phylogeny of the Agaricales is presented based on a multilocus analysis of a six-gene region supermatrix. Bayesian analyses of 5611 nucleotide characters of rpb1, rpb1-intron 2, rpb2 and 18S, 25S, and 5.8S ribosomal RNA genes recovered six major clades, which are recognized informally and labeled the Agaricoid, Tricholomatoid, Marasmioid, Pluteoid, Hygrophoroid and Plicaturopsidoid clades. Each clade is discussed in terms of key morphological and ecological traits. At least 11 origins of the ectomycorrhizal habit appear to have evolved in the Agaricales, with possibly as many as nine origins in the Agaricoid plus Tricholomatoid clade alone. A family-based phylogenetic classification is sketched for the Agaricales, in which 30 families, four unplaced tribes and two informally named clades are recognized.
An overview of the phylogeny of the Agaricales is presented based on a multilocus analysis of a six-gene region supermatrix. Bayesian analyses of 5611 nucleotide characters of rpb1, rpb1-intron 2, rpb2 and 18S, 25S, and 5.8S ribosomal RNA genes recovered six major clades, which are recognized informally and labeled the Agaricoid, Tricholomatoid, Marasmioid, Pluteoid, Hygrophoroid and Plicaturopsidoid clades. Each clade is discussed in terms of key morphological and ecological traits. At least 11 origins of the ectomycorrhizal habit appear to have evolved in the Agaricales, with possibly as many as nine origins in the Agaricoid plus Tricholomatoid clade alone. A family based phylogenetic classification is sketched for the Agaricales, in which 30 families, four unplaced tribes and two informally named clades are recognized ...
Yeasts represent one of the growth forms of fungi. They are unicellular, and reproduce mostly by budding or less often by fission. Most medically important yeasts originate from the division of Ascomycota or from the division of Basidiomycota. A simple biochemical reaction, the urease test, can usually distinguish the yeasts from each of the divisions. Ascomycetous yeasts are usually urease negative, whereas basidiomycetous yeasts are urease positive.
Tettelin, H., Agostoni Carbone, M. L., Albermann, K., Albers, M., Arroyo, J., Backes, U., Barreiros, T., Bertani, I., Bjourson, A. J., Bruckner, M., Bruschi, C. V., Carignani, G., Castagnoli, L., Cerdan, E., Clemente, M. L., Coblenz, A., Coglievina, M., Coissac, E., Defoor, E., Del Bino, S., Delius, H., Delneri, D., de Wergifosse, P., Dujon, B., Kleine, K., et, a. l. .. (1997). The nucleotide sequence of Saccharomyces cerevisiae chromosome VII. Nature 387:81-84.9169869 ...
The pitch canker pathogen, Fusarium circinatum , has major impacts on production in pine nurseries and plantations in South Africa. Thus far, efforts to reduce local spread have focused on rouging of infected pines and sanitation to eliminate local sources of inoculum. Although the host range of F. circinatum was thought to be limited to pines and Douglas-fir, recent studies in California indicate ...
Using the labor of dozens of undergraduate students, scientists have built a customized yeast chromosome from scratch. It's a milestone in the rapidly
Agaricales Capnoides Euagarics Floor Fungus Hypholoma Nobody Photos Teleomorph: 1 assigned downloads, like Smokey Gilled Woodlover mushroom (Hypholoma capnoides), Untergroeningen, Baden-Wuerttemberg, Germany, Europe from stock-photos
Cortinarius cumatilis posliiniseitikki porslinsbl spindling galambk k p kh l sgomba Wavy Webcap pavučinovec modrofialov pavučinec modrolil kv praktsl rsopp Cortinaire bleu violet Taubenblauer ...
Text is available under the Creative Commons Attribution-ShareAlike License; additional terms may apply. By using this site, you agree to the Terms of Use and Privacy Policy. ...
At Perfect Brewing Supply you will find experienced homebrewing guys and gals focused on making excellent beer, wine, cider and soda at home. We are your neighb
Pictures © 2004, 2007, 2011, 2018 J.K. Lindsey. Please report errors to [email protected] Translations. Back to Ecology of Commanster. ...
genital fungus - MedHelps genital fungus Center for Information, Symptoms, Resources, Treatments and Tools for genital fungus. Find genital fungus information, treatments for genital fungus and genital fungus symptoms.
A(z) No Szmoking:Hol a pénz,hol a.. című videót hzsuzsa nevű felhasználó töltötte fel a(z) zene kategóriába. Eddig 181 alkalommal nézték meg.
Il lievito è diventato popolarissimo tra i carpisti inglesi perché è un prodotto che ha permesso molte catture record in acque oramai considerate assuefatte da ogni genere di attrattore. Lo YEST POWDER subisce particolari trattamenti, tra cui il seccaggio spray, per mantenere inalterate le straordinarie qualità organolettiche prerogative del lievito: energetico, ricco di enzimi e del complesso vitaminico del gruppo B, è indispensabile per lo sviluppo ed il regolare funzionamento del metabolismo della carpa. Dosaggio: 20 - 30gr per kg di MIX
A fungus is a simple organism, or living thing, that is neither a plant nor an animal. When there is more than one fungus they are called fungi. Some familiar fungi are…
Hello all, There is a fungus problem with my CPs now. The fungus concerned is this mould-like, powdery substance which attacks the bases of dead growt
真菌界(學名:Fungi,複數可為fungi或funguses[3])又稱菌物界,是真核生物中的一大類群,包含酵母、黴菌之類的微生物以及為人熟知的菇類。真菌自成一界,獨立於植物、動物和其他真核生物。 ...
Alper, I; Michel, F; Labrie, S (2011). "Ribosomal DNA Polymorphisms in the Yeast Geotrichum candidum". Fungal Biology. 115 (12 ... Fungal growth can be supported by D-glucose, D-mannose, D-xylose, L-sorbose, D-fructose, D-galactose, sucrose, D-mannitol, ...
DNA replication occurs when one double-stranded DNA molecule creates two single strands of DNA, each of which is a template for ... Because RIP is so efficient at detecting and mutating repeats, fungal biologists often use it as a tool for mutagenesis. A ... These reactants can lead to both single-stranded DNA breaks and double-stranded DNA breaks. Third, bonds in DNA eventually ... It is a DNA methyltransferease-like protein, that when mutated or knocked out results in loss of RIP. Deletion of the rid ...
"Fungal Genetics". Nature.com. Macmillan Publishers Limited. Retrieved 9 November 2015.. *^ Beadle GW, Tatum EL (1941). "Genetic ... Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector. Transduction is a common ... Both DNA and RNA viruses can undergo recombination. When two or more viruses, each containing lethal genomic damage infect the ... H. volcani forms cytoplasmic bridges between cells that appear to be used for transfer of DNA from one cell to another in ...
Fungal diversity[edit]. Exploration of fungal diversity in situ has also benefited from next-generation DNA sequencing ... fungal diversity in forest soils,[6] stratification of fungal communities in soil horizons,[7] and fungal succession on ... Seifert, KA (2009). "Progress towards DNA barcoding of fungi". Mol Ecol Resour. 9: 83-89. doi:10.1111/j.1755-0998.2009.02635.x ... The majority of fungal ecology research leveraging next-generation sequencing approaches involves sequencing of PCR amplicons ...
Molecular phylogeny of the Blastocladiomycota (Fungi) based on nuclear ribosomal DNA. Fungal Biology 115:381-392. Kendrick, ... Fungal Diversity. 90 (1): 135-159. doi:10.1007/s13225-018-0401-0. Hibbett DS et al. 2007. A higher-level phylogenetic ...
... based on nuclear ribosomal DNA". Fungal Biology. 115: 381-392. doi:10.1016/j.funbio.2011.02.004. 6. Allomyces. Phillip W. ...
... or DNA-based methods such as molecular phylogenetics or DNA barcoding. The existence of extremely similar species may cause ... Fungal Diversity. 45 (1): 99-130. doi:10.1007/s13225-010-0070-0.. ... The increased use of DNA sequence in the investigation of organismal diversity (also called Phylogeography and DNA barcoding) ... Analysis of DNA sequences is becoming increasingly standard for species recognition and may in many cases be the only useful ...
Molecular phylogeny of the Blastocladiomycota (Fungi) based on nuclear ribosomal DNA. Fungal Biology 115: 381-392. James, T.Y ...
Ochricompactae" (PDF). Fungal Diversity. 28: 15-40. Eberhardt U, Verbeken A (2004). "Sequestrate Lactarius species from ... Russulales; Basidiomycota) in Alaska, based on soil and sporocarp DNA" (PDF). Molecular Ecology. 18 (10): 2213-2227. doi: ... doi:10.1111/j.1469-8137.1995.tb04309.x. Twieg BD, Durall DM, Simard SW (2007). "Ectomycorrhizal fungal succession in mixed ... Rinaldi AC, Comandini O, Kuyper TW (2008). "Ectomycorrhizal fungal diversity: separating the wheat from the chaff" (PDF). ...
Fungal spores found in the body of the nail resembled the characteristic morphology of N. sphaerica. DNA sequence analysis ... Fungal spores trapped on the agar strips were developed and counted. They were then cultured into isolates allowing for ... A woman in south India was diagnosed with a fungal corneal ulcer after being hit in the eye from a cow's tail. Analysis of ... It was hypothesized that this special case of fungal corneal ulcer was caused by transfer of spores to the patients eye from ...
... morphological and multilocus DNA sequence analyses". Fungal Diversity. 58 (1): 73-101. doi:10.1007/s13225-012-0184-7. Neves MA ... Fungal Diversity. 55 (1): 109-123. doi:10.1007/s13225-012-0154-0. "Phylloporus on Boletineae: Surveys and Revisions". " ...
... morphological and multilocus DNA sequence analyses". Fungal Diversity. 58: 73-101. doi:10.1007/s13225-012-0184-7. S2CID ... Fungal Diversity. 55 (1): 109-23. doi:10.1007/s13225-012-0154-0. S2CID 17272328. Zheng NK, Tang LP, Li YC, Tolgor B, Zhu XT, ...
... is an N-terminal domain involved in copper-dependent DNA binding. It is named for its resemblance to a fist. It can ... be found in some fungal transcription factors. These proteins activate the transcription of the metallothionein gene in ... which allows DNA binding. Copper fist definition Turner RB, Smith DL, Zawrotny ME, Summers MF, Posewitz MC, Winge DR (July 1998 ...
Quantitative PCR is a DNA-based molecular method that can identify and quantify fungal species. The Environmental Relative ... The ERMI consists of a list of 36 fungal species commonly associated with damp houses that can be measured using qPCR.[17][16] ... Infants may develop respiratory symptoms as a result of exposure to Penicillium, a fungal genus. Signs of mold-related ... Mold (American English) or mould (British English), also sometimes referred to as mildew, is a fungal growth that develops on ...
This species is part of the community of fungal successors of decaying wood. A Finnish study found that it fruited most ... "Phylogenetic relationships in European Ceriporiopsis species inferred from nuclear and mitochondrial ribosomal DNA sequences". ... Yu, Chang-Jun; Zuo, Li; Dai, Yu-Cheng (2005). "Three polypores from Xizang new to China" (PDF). Fungal Science. 20 (3-4): 61-68 ... "Skeletocutis nothofagi Rajchenb". The Global Fungal Red List Initiative. IUCN. Retrieved 30 September 2016. Fries, Elias Magnus ...
"Phylogeny of the genus Agaricus inferred from restriction analysis of enzymatically amplified ribosomal DNA". Fungal Genet Biol ... The fungal genus Agaricus contains about 200 species worldwide. Agaricus abruptibulbus Peck (1905) - abruptly bulbous agaricus ... Kerrigan Section Duploannulatae Based on DNA analysis of ITS1, ITS2, and 5.8S sequences, this section (also known as section ...
Most are designed to mimic single DNA bases, nucleosides, or nucleotides in order to nonspecifically target DNA. These have ... several different medicinal uses including anti-cancer, anti-viral, and anti-fungal applications. Abiotic foldamers are again ...
Fungal spores found in the body of the nail resembled the characteristic morphology of N. sphaerica. DNA sequence analysis ... N. sphaerica was first identified by E. W. Mason in 1927.[1] In 1913, S. F. Ashby and E. F. Shepherd isolated fungal cultures ... A study shows N. sphaerica to be the most abundant airborne fungal species found in various urban sites in Singapore. Air ... Fungal spores trapped on the agar strips were developed and counted. They were then cultured into isolates allowing for ...
DNA sequencing data reveals the presence of two major groups representing cryptic species in F. subglutinans. These were ... Fusarium subglutinans is a fungal plant pathogen. Fusarium subglutinans is the anamorph of Gibberella fujikuroi. Fusarium ...
... also undergoes conversion into 5-fluorodeoxyuridinemonophosphate which inhibits fungal DNA synthesis. Flucytosine ... Serious fungal infections may occur in those who are immunocompromised. These people benefit from combination therapy including ... Flucytosine must not be used as a sole agent in life-threatening fungal infections due to relatively weak antifungal effects ...
As DNA sequencing costs dropped, they used population genetics to recognize fungal species and describe them, based solely on ... Taylor, John W. (December 2011). "One Fungus = One Name: DNA and fungal nomenclature twenty years after PCR". IMA Fungus. 2 (2 ... and Fungal Kingdom: Threats and Opportunities (2019-2024). In 1990, Taylor's lab group published protocols for fungal PCR that ... DNA sequence data were then used to detect recombination in fungi for which sex had never been observed, despite years of ...
Spectrum of fungal susceptibility[edit]. Cycloheximide has been used to isolate dermatophytes and inhibit the growth of fungi ... Translation is halted via the addition of cycloheximide, and the DNA/RNA in the cell is then nuclease treated. The ribosome- ... Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth ...
Although the replication and transcription of DNA is highly standardized in eukaryotes, the same cannot be said for their ... Besides plants and animals, the evolutionary history of various fungal species is dotted by past and recent whole-genome ... Lidzbarsky, G. A.; Shkolnik, T.; Nevo, E. (2009). Idnurm, A. (ed.). "Adaptive Response to DNA-Damaging Agents in Natural ... Indeed, homoploid speciation (hybrid speciation without a change in chromosome number) has been evidenced for some fungal ...
Lücking, Robert; Nadel, Miko; Araujo, Elena; Gerlach, Alice (2020). "Two decades of DNA barcoding in the genus Usnea ( ... Fungal Diversity. 84 (1): 101-117. doi:10.1007/s13225-017-0379-z. Ohmura 2001, p. 8. Ohmura 2001, p. 9. Ohmura 2001. Ohmura ... Parmeliaceae): how useful and reliable is the ITS?". Plant and Fungal Systematics. 65 (2): 303-357. doi:10.35535/pfsyst-2020- ...
Animal Genome Size Database Plant DNA C-values Database Fungal Genome Size Database. ... nucleus Comparative genomics Genome Genome size Human genome Junk DNA Noncoding DNA Plant DNA C-values Database Selfish DNA ... to picograms of DNA and vice versa are: genome size (bp) = (0.978 x 109) x DNA content (pg) = genome size (bp) / (0.978 x 109) ... C-value is the amount, in picograms, of DNA contained within a haploid nucleus (e.g. a gamete) or one half the amount in a ...
In meiosis, the DNA is replicated only once, while the cell divides twice. DNA replication only occurs before meiosis I. DNA ... The vacuoles of plant cells and fungal cells are usually larger than those of animal cells. Ribosomes: The ribosome is a large ... The mitochondrial genome is a circular DNA molecule distinct from the nuclear DNA. Although the mitochondrial DNA is very small ... DNA) and ribonucleic acid (RNA). Cells use DNA for their long-term information storage. The biological information contained in ...
A single fungal serpin has been characterized to date: celpin from Piromyces spp. strain E2. Piromyces is a genus of anaerobic ... Ahn JW, Atwell BJ, Roberts TH (2009). "Serpin genes AtSRP2 and AtSRP3 are required for normal growth sensitivity to a DNA ... Two other Arabidopsis serpins, AtSRP2 (At2g14540) and AtSRP3 (At1g64030) appear to be involved in responses to DNA damage. ... Dockerins are commonly found in proteins that localise to the fungal cellulosome, a large extracellular multiprotein complex ...
Fungal Genet. Biol. 43 (7): 511-29. doi:10.1016/j.fgb.2006.02.006. PMID 16626980. Chandelier A, Abras S, Laurent F, Debruxelles ... "Genetic characterization of the natural hybrid species Phytophthora alni as inferred from nuclear and mitochondrial DNA ...
"A method for the large scale isolation of high transformation efficiency fungal genomic DNA". FEMS Microbiology Letters. 145 (2 ... Cerniglia, C. E.; Mahaffey, W.; Gibson, D. T. (1980). "Fungal oxidation of benzo\a]pyrene: Formation of (−)-trans-7,8-dihydroxy ... Pothuluri, J. V.; Freeman, J. P.; Evans, F. E.; Cerniglia, C. E. (1992). "Fungal metabolism of acenaphthene by Cunninghamella ... Methods for efficient C. elegans genomic DNA isolation and transformation have been developed. The cytochrome P450 of C. ...
Lindner DL, Gargas A, Lorch JM, Banik MT, Glaeser J, Kunz TH, Blehert DS (2011-03-18). "DNA-based detection of the fungal ... Researchers suspect through DNA analysis that the source of infection in this individual originated in the Eastern U.S. This ... "Fungal disease sapping bats all over Georgia". Savannah Now. 19 May 2016. Retrieved 21 May 2016. "Bats affected by WNS". White- ... White-nose syndrome (WNS) is a fungal disease in North American bats which has resulted in the dramatic decrease of the bat ...
1 DNA-Viren *1.1 Doppelsträngige DNA-Viren (dsDNA: double stranded DNA). *1.2 Einzelsträngige DNA-Viren (ssDNA: single stranded ... Fungal Prions (Pilz-Prionen). Vorgeschlagene oder nicht-offizielle Virustaxa[Bearbeiten , Quelltext bearbeiten]. Die Virus- ... Viren mit DNA-Genom bilden keine taxonomische Einheit. Doppelsträngige DNA-Viren (dsDNA: double stranded DNA)[Bearbeiten , ... Einzelsträngige DNA-Viren (ssDNA: single stranded DNA)[Bearbeiten , Quelltext bearbeiten]. Viren mit Einzelstrang-DNA-Genom ...
... is an Ascomycete fungus that causes ash dieback, a chronic fungal disease of ash trees in Europe ... based on morphological and DNA sequence comparisons, Chalara fraxinea was suggested to be the asexual stage (anamorph) of the ... Fungal Ecology. 5 (2): 147-153. doi:10.1016/j.funeco.2011.10.004.. ...
Many conifers have distinctly scented resin, secreted to protect the tree against insect infestation and fungal infection of ... but recent research into DNA sequences suggests that this interpretation leaves the Pinales without Taxales as paraphyletic, ...
"Same DNA deletion paves paths to autism, schizophrenia , Spectrum". Spectrum. 2016-10-18. Retrieved 2016-11-13.. ... The studies on fungal evolutionary genomics will further elucidate the earliest stages of divergence as a result of gene flow, ... Studies on fungal evolutionary genomics have shown pleiotropic traits that simultaneously affect adaptation and reproductive ... "Fungal evolutionary genomics provides insight into the mechanisms of adaptive divergence in eukaryotes". Molecular Ecology. 23 ...
5,0 5,1 5,2 5,3 5,4 5,5 de Souza, P. M. et al., (2015). A biotechnology perspective of fungal proteases. Braz. J. Microbiol., ... Ghatak, S. et al., (2015). A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis. JBT, nr 24(4), ... mida kasutatakse DNA ja RNA eraldamiseks bioloogilisest materjalist[12][13] ja papaiin, mida kasutatakse rakkude isoleerimiseks ...
Zhao, ZJ; Tong, HR; Zhou, L; Wang, EX; Liu, QJ (2010). "FUNGAL COLONIZATION OF PU‐ERH TEA IN YUNNAN". Journal of Food Safety. ... "Phylogeography of Camellia taliensis (Theaceae) inferred from chloroplast and nuclear DNA: insights into evolutionary history ... The bacterial and fungal cultures found in the fermenting piles were found to vary widely from factory to factory throughout ... Aged raw pu'erh: Aged pu'er should never smell moldy, musty, or strongly fungal, though some pu'erh drinkers consider these ...
Descriptions of what is believed to be leprosy are included.[9][10] A proven ancient human case was verified by DNA taken from ... As late as the 17th century in Europe, persons with severe favus and similar fungal diseases (and potentially also with severe ... "DNA of Jesus-Era Shrouded Man in Jerusalem Reveals Earliest Case of Leprosy," ScienceDaily, 2009-12-16. Retrieved 2010-01-31. ... DNA analysis has been applied to the origins and history of leprosy. Geneticists in 2005 used comparative genomics to study ...
Main articles: RNA and DNA. The major difference between RNA and DNA is the presence of a hydroxyl group at the 2'-position of ... This forces an RNA double helix to change from a B-DNA structure to one more closely resembling A-DNA. ... DNA and proteins seemed the dominant macromolecules in the living cell, with RNA only aiding in creating proteins from the DNA ... The overall structure of RNA and DNA are immensely similar-one strand of DNA and one of RNA can bind to form a double helical ...
In 1871, Paul Kummer created Eccilia, Leptonia and Nolanea to this end at the same time as Entoloma, but DNA studies show these ... Leiden & Utrecht: National Herbarium of The Netherlands & the CBS Fungal Biodiversity Centre. 23 (2): 147-176. doi:10.3767/ ...
DNA sequencing, a method developed by Walter Gilbert and Frederick Sanger in 1977,[5] caused a rapid change the development of ... Bacterial infections are treated with antibacterials (often called antibiotics) whereas fungal and viral infections are treated ... Most DNA viruses assemble in the nucleus while most RNA viruses develop solely in cytoplasm.[22][23] ... Once the virus has gained access to the host's cells, the virus' genetic material (RNA or DNA) must be introduced to the cell. ...
Bacteria also use DNA adenine methylation (rather than DNA cytosine methylation) as an epigenetic signal. DNA adenine ... For more details on this topic, see Fungal prions.. Prions are infectious forms of proteins. In general, proteins fold into ... DNA damage can also cause epigenetic changes.[27][28][29] DNA damage is very frequent, occurring on average about 60,000 times ... Chromatin is the complex of DNA and the histone proteins with which it associates. If the way that DNA is wrapped around the ...
As with all long-lived plant and fungal species, no individual part of a clonal colony is alive (in the sense of active ... as determined by DNA samples taken from pollen collected from the sediment of a nearby lake. Individual trees in this group ...
"Platypus Fungal Disease". Department of Primary Industries and Water, Tasmania. 29 August 2008. Archived from the original on 7 ... "The platypus is not a rodent: DNA hybridization, amniote phylogeny and the palimpsest theory". Philosophical Transactions: ...
DNA, RNA, etc.), or to complex macromolecular assemblies.[3] Finally, while aspects of the lab techniques applied to peptides ... fungal peptides, invertebrate peptides, amphibian/skin peptides, venom peptides, cancer/anticancer peptides, vaccine peptides ...
True birds first appeared during the Cretaceous period, around 120 million years ago.[5] DNA-based evidence finds that birds ... Many species will spread out their wings and expose them to direct sunlight and this too is thought to help in reducing fungal ... fossils and DNA have all been brought to bear on the problem, but no strong consensus has emerged. More recently, new fossil ... "Ancient DNA reveals elephant birds and kiwi are sister taxa and clarifies ratite bird evolution". Science. 344 (6186): 898-900 ...
Green signal from anti-tubulin antibody conjugated with Alexa Fluor 488) and nuclei (blue signal from DNA stained with DAPI) in ... It is used for localizing and identifying the presence of filamentary fungal elements in the corneal stroma in cases of ...
"Social Bats Pay a Price: Fungal Disease, White-Nose Syndrome ... Extinction?". The National Science Foundation. 3 July 2012. ... a conclusion supported by a 2005 DNA study.[18] A 2013 phylogenomic study supported the two new proposed suborders.[15] ... "Wanted DNA samples from Nyctimene or Paranyctimene Bats" (PDF). The New Guinea Tropical Ecology and Biodiversity Digest. 3: 10 ... "A Nuclear DNA Phylogenetic Perspective on the Evolution of Echolocation and Historical Biogeography of Extant Bats (Chiroptera ...
Aging of the hair follicle appears to be primed by a sustained cellular response to the DNA damage that accumulates in renewing ... Causes of hair loss that occurs with scarring or inflammation include fungal infection, lupus erythematosus, radiation therapy ... This damage response involves the proteolysis of type XVII collagen by neutrophil elastase in response to the DNA damage in the ...
Twenty-four products were tested by DNA barcoding as part of the investigation, with all but five containing DNA that did not ... The scope of herbal medicine is sometimes extended to include fungal and bee products, as well as minerals, shells and certain ... "DNA barcoding detects contamination and substitution in North American herbal products". BMC Medicine. 11: 222. doi:10.1186/ ... "Deep Sequencing of Plant and Animal DNA Contained within Traditional Chinese Medicines Reveals Legality Issues and Health ...
Most fungal cellulases have a two-domain structure, with one catalytic domain and one cellulose binding domain, that are ... DNA glycosylases: Oxoguanine glycosylase. Retrieved from "https://en.wikipedia.org/w/index.php?title=Cellulase&oldid=985917139" ...
In systemic lupus there are autoantibodies to DNA, which cannot evoke a T cell response, and limited evidence for T cell ... Recurrent bacterial and fungal infections and chronic inflammation of the gut and lungs are seen in chronic granulomatous ... Induction of autoantibodies against tyrosinase-related proteins following DNA vaccination: Unexpected reactivity to a protein ... DNA, C1q, IgGFc, Ro, Con. A receptor, Peanut agglutinin receptor(PNAR)). This fact gave rise to the idea that spontaneous ...
Since its chloroplast DNA is that of pomelo, it was likely the hybrid pomelo, perhaps a BC1 pomelo backcross, that was the ... Greasy spot, a fungal disease caused by the Mycosphaerella citri, produces leaf spots and premature defoliation, thus reducing ...
a b Bernstein H, Bernstein C, Michod RE (2012). "DNA repair as the primary adaptive function of sex in bacteria and eukaryotes ... Beckett, A., Heath, I. B., and Mclaughlin, D. J. (1974). An Atlas of Fungal Ultrastructure. Longman, Green, New York. ... 1-49 in DNA Repair: New Research, Sakura Kimura and Sora Shimizu (eds.). Nova Sci. Publ., Hauppauge, N.Y. ISBN 978-1-62100-808- ... For example, the word "protist pathogen" may be used to denote any disease-causing organism that is not plant, animal, fungal, ...
This may allow fungal spores to gain admittance and attack the tree. Dutch elm disease is caused by a fungus (Ophiostoma ... "Scientists to capture DNA of trees worldwide for database". USA Today. Retrieved 2012-09-29 ...
"In Kimura H, Suzuki A (eds.). New Research on DNA Damages. New York: Nova Science Publishers. pp. 1-47. ISBN 978-1-60456-581-2 ... Lam K, Thu K, Tsang M, Moore M, Gries G (September 2009). "Bacteria on housefly eggs, Musca domestica, suppress fungal growth ... Oxidative DNA damage 8-hydroxydeoxyguanosine in houseflies was found in one study to increase with age and reduce life ... Holmes GE, Bernstein C, Bernstein H (September 1992). "Oxidative and other DNA damages as the basis of aging: a review". ...
Fungal sepsis accounts for approximately 5% of severe sepsis and septic shock cases; the most common cause of fungal sepsis is ... and CpG bacterial DNA. These PAMPs are recognized by the pattern recognition receptors (PRRs) of the innate immune system, ... If fungal infection is suspected, an echinocandin, such as caspofungin or micafungin, is chosen for people with severe sepsis, ... Infections leading to sepsis are usually bacterial but may be fungal or viral.[21] Gram-positive bacteria were the primary ...
2004). "The genome sequence of silkworm, Bombyx mori". DNA Research. 11 (1): 27-35. doi:10.1093/dnares/11.1.27. PMID 15141943. ... the Beauveria bassiana fungal infection mentioned above. It is believed to dispel flatulence, dissolve phlegm, and relieve ... Bombyx mori from Chinese Bombyx mandarina and paternal inheritance of Antheraea proylei mitochondrial DNA" (PDF). Molecular ...
... or by oxidizing DNA or proteins.[53] Damage to DNA can cause mutations and possibly cancer, if not reversed by DNA repair ... Antioxidants are an especially important class of preservatives as, unlike bacterial or fungal spoilage, oxidation reactions ... protect DNA from oxidative stress. It has been proposed that polymorphisms in these enzymes are associated with DNA damage and ... Oxidative damage in DNA can cause cancer. Several antioxidant enzymes such as superoxide dismutase, catalase, glutathione ...
Template:DNA and protein biosynthesis navs(edit talk links history)- Genetics ({{Protein biosynthesis navs}}, R) ...
... it was estimated that the total global environmental DNA sequencing effort had produced less than 1 percent of the total DNA ... In mutualistic situations the plant often exchanges hexose sugars for inorganic phosphate from the fungal symbiont. It is ... A common marker for human microbiome studies is the gene for bacterial 16S rRNA (i.e. "16S rDNA", the sequence of DNA which ... Microbial DNA inhabiting a person's human body can uniquely identify the person. A person's privacy may be compromised if the ...
ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains. ... Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ... Fungal and Yeast Genomic DNA * Penicillium rubens Biourge (ATCC® 28089D-2™) ATCC® Number: 28089D-2™ Designation: Genomic DNA ... Designation: genomic DNA from strain RP-78 (ATCC MYA-4764) Product Format: dried Total DNA: Approximately 2 µg in 1X TE buffer ...
ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains. ... Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ... Fungal and Yeast Genomic DNA Fast STR Profiling Service Authentication of cell lines via STR profile analysis is becoming a ...
ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains. ... Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ... Fungal and Yeast Genomic DNA * Debaryomyces hansenii (Zoph) Lodder et Kreger-van Rij (ATCC® 36239D-5™) ATCC® Number: 36239D-5™ ... This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other ...
Sequencing of a high number of fungal genomes has become possible due to the development of next generation sequencing ... Fauchery L., Uroz S., Buée M., Kohler A. (2018) Purification of Fungal High Molecular Weight Genomic DNA from Environmental ... CTAB-based DNA extraction Sucrose density gradient ultracentrifugation Fruiting body This is a preview of subscription content ... Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74(12):5463- ...
Short name: Zn2-C6_fun-type_DNA-bd Overlapping homologous superfamilies *Zn(2)-C6 fungal-type DNA-binding domain superfamily ( ... Zn(2)-C6 fungal-type DNA-binding domain (IPR001138) *Maltose fermentation regulatory protein, DNA-binding domain (IPR020448) ... Zn(2)-C6 fungal-type DNA-binding domain (IPR001138). ... DNA recognition by GAL4: structure of a protein-DNA complex.. ... GO:0006355 regulation of transcription, DNA-templated Molecular Function. GO:0000981 DNA-binding transcription factor activity ...
DNA barcoding Microbial DNA barcoding Pollen DNA barcoding DNA barcoding in diet assessment Consortium for the Barcode of Life ... as primary fungal DNA barcode, the necessity of establishing a second DNA barcode marker was expressed. Several attempts were ... Fungal DNA barcoding can help to identify and associate anamorphic and teleomorphic stages of fungi, and through that to reduce ... Fungal DNA barcoding is the process of identifying species of the biological kingdom Fungi through the amplification and ...
Zn(2)-C6 fungal-type DNA-binding domain superfamily (IPR036864). Short name: Zn2-C6_fun-type_DNA-bd_sf ... DNA recognition by GAL4: structure of a protein-DNA complex.. Nature 356 408-14 1992 ... of fungal transcriptional regulatory proteins contains a Cys-rich motif that is involved in zinc-dependent binding of DNA. The ... GO:0006355 regulation of transcription, DNA-templated Molecular Function. GO:0000981 RNA polymerase II transcription factor ...
Fungal Model to Shed New Light on Human DNA. The National Institutes of Health has awarded Professor Michael Hood a $444,651 ... Fungal Model to Shed New Light on Human DNA. ... There are other advantages to anther smut as well: the fungal ... The problem lies in the unusual nature of the human male sex chromosomes, the only two on the long string of human DNA that are ... Its a question that has baffled scientists since they began understanding DNA, and is one that Michael E. Hood, associate ...
DNA addition or deletion is associated with a major karyotype polymorphism in the fungal phytopathogen Colletotrichum ... All ten chromosome-specific DNA probes hybridised to a 2.0 Mb chromosome in all races of C. gloeosporioides causing Type A ... These ten probes also hybridised only to restriction-digested DNA of race 3 and were thus both chromosome- and strain-specific ... A gene library was prepared specifically from the 1.2 Mb minichromosome and ten independent DNA clones unique to this ...
Although the fungal order Mortierellales constitutes one of the largest classical groups of Zygomycota, its phylogeny is poorly ...
Further reports about: , CONS , Conservation Science , DNA , DNA sequence , End User Development , Lips , Panama , STRI , ... CONS »Conservation Science »DNA »DNA sequence »End User Development »Lips »Panama »STRI »Sustainable bioenergy »evolutionary ... DNA barcoding reveals 5 undiscovered frog species among 30 wiped out by fungal epidemic in Panama. 20.07.2010 ... "Our DNA barcode data reveal new species even at this relatively well-studied site, yet the field sampling shows that many of ...
The fungal mat was ground into a fine powder with a sterile pestle. Fungal DNA was then extracted and purified using serial ... Fungal DNA is amplified using universal fungal primers (ITS1 and ITS4) directed towards the rRNA gene. The rRNA gene is a ... Universal fungal primers ITS1 and ITS4, directed to the conserved regions of ribosomal DNA, were used to amplify DNA from ... By use of universal fungal primers and a biotinylated universal probe, all fungal DNA was amplified and bound to strepavidin- ...
Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood.. J Löffler, H Hebart, U ... The QIAmp Tissue kit was the commercially available assay that yielded the same sensitivity and purity of fungal DNA release as ... Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood. ... Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood. ...
Overall, we revealed here an unexpected plasticity and dynamics of P. jirovecii mitochondrial DNA that vary according to P. ... Hierarchical clustering analysis (HCA) allowed us to define five major clusters, significantly associated with fungal load (p= ... jirovecii physiological state with a decreased number of copies when the fungal load is low. This suggests the existence of a ... jirovecii physiological state with a decreased number of copies when the fungal load is low. This suggests the existence of a ...
Antibiotic eye drops and steroid eye drops for the treatment of penetrating keratoplasty can cause atypical fungal presentation ... The cause of cacosmia in the present case was fungal dacryocystitis. ... The fungi isolated from the contents of lacrimal sac were identified by DNA sequencing as Wickerhamomyces anamalus (Pichia ... Pathological examination confirmed a diagnosis of fungal dacryocystitis based on the fungal hyphae observed on the excised ...
The identification of biomarkers for Alzheimers disease is important for patient management and to assess the effectiveness of clinical intervention. Cerebrospinal fluid (CSF) biomarkers constitute a powerful tool for diagnosis and monitoring diseas
... fungal DNA and allergens in classroom dust. Dan Norbäck, Pawel Markowicz, Gui-Hong Cai, Zailina Hashim, Faridah Ali, Yi-Wu ... fungal DNA and allergens in classroom dust. Dan Norbäck, Pawel Markowicz, Gui-Hong Cai, Zailina Hashim, Faridah Ali, Yi-Wu ... fungal DNA and allergens in classroom dust. Dan Norbäck, Pawel Markowicz, Gui-Hong Cai, Zailina Hashim, Faridah Ali, Yi-Wu ... fungal DNA and allergens in classroom dust Message Subject (Your Name) has sent you a message from European Respiratory Society ...
... detection of medically important fungi in clinical samples we established a combination of commercially available kits for DNA ... Detection of PCR-amplified fungal DNA by using a PCR-ELISA system Med Mycol. 1998 Oct;36(5):275-9. doi: 10.1080/ ... all patients with proven and probable invasive fungal infection were found to be PCR-positive. Thus the PCR plate assay was ... detection of medically important fungi in clinical samples we established a combination of commercially available kits for DNA ...
GM levels of Aspergillus/Penicillium DNA, Aspergillus versicolor DNA, Stachybotrys chartarum DNA and Streptomyces DNA were all ... Five sequences of fungal DNA were analyzed by quantitative PCR. MVOCs were sampled on charcoal tubes in 42 TSC flights, and 17 ... Furry pet allergens, fungal DNA and microbial volatile organic compounds (MVOCs) in the commercial aircraft cabin environment. ... Fungal DNA, furry pet allergens and volatile organic compounds of microbial origin (MVOC) were measured on board. Cat (fel d1 ...
This protocol is based on the fact that we struggled for a long time to obtain high purity DNA from the fungal material, ...
CE IVD-marked diagnosis of pathogens of 200 bacterial and 65 fungal genera in tissue biopsies, blood and other body fluids ... Extraction of DNA from only intact bacteria and fungi. *Detection of bacterial and fungal DNA by broad-range 16S/18S rRNA gene ... The removal of human DNA and free-circulating microbial DNA ensures high detection specificity of exclusively DNA of intact ... Culture-Independent Diagnosis of Bacterial and Fungal DNA in Blood, Other Body Fluids and Tissues. SepsiTest-UMD CE IVD: ...
Fungal DNA Kit. Purified DNA from various fungal samples was isolated with the E.Z.N.A.® Fungal DNA Kit. DNA was extracted from ... E.Z.N.A.® Fungal DNA Mini Kit. Products. , Genomic DNA Top Sellers. , Plant and Fungal. , E.Z.N.A.® Fungal DNA Mini Kit. ... E.Z.N.A.® HP Fungal DNA Kit. Efficient recovery of genomic DNA up to 60 kb in size from fresh and dried fungal tissue samples ... E.Z.N.A.® Fungal DNA Mini Kit allows for the rapid and reliable isolation of high-quality total cellular DNA from a wide ...
E.Z.N.A.® HP Fungal DNA Kit. Products. , Genomic DNA Top Sellers. , Plant and Fungal. , E.Z.N.A.® HP Fungal DNA Kit. ... HP Fungal DNA Kit. Purified genomic DNA from various fungi was isolated with the E.Z.N.A.® HP Fungal DNA Kit. DNA was extracted ... Fungal DNA Kit is designed for efficient recovery of genomic DNA from fresh and dried fungal tissue samples rich in ... Genomic DNA (200 ng of total purified DNA) was analyzed on a 0.8% agarose gel to demonstrate yield and quality of the DNA. M: ...
The KilA-N domain is suggested to be homologous to the fungal DNA-binding APSES domain. We provide evidence for the KilA-N and ... The amino-terminal module of the Bro proteins is another, distinct DNA-binding domain (the Bro-N domain) that is present in ... The phyletic range and the conserved DNA-binding domains of the viral regulatory proteins of the poxvirus D6R/N1R and ... Using computational analysis, we show that the amino-terminal module of the D6R/N1R proteins defines a novel, conserved DNA- ...
Methods: Measurements of fungal DNA (f-DNA) were performed by quantitative polymerase chain reaction (PCR) in classrooms ... Aim: To assess how indoor moulds confirmed by fungal DNA can affect respiratory health and pulmonary function in school- ... Quantitative PCR analysis of fungal DNA in European classrooms and association with respiratory health and pulmonary function ... Results: The highest mean concentrations of total f-DNA were found in France and Norway (geometric mean 5.51 and 4.19 x 105 CE/ ...
DNA, Fungal Fungi Genes, RRNA Mycological Typing Techniques Polymerase Chain Reaction Sequence Analysis, DNA ... Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing ... Three commonly used DNA extraction methodologies (UltraClean Soil kit, High Pure PCR Template kit, and EluQuik/DNeasy kit) were ... Fungal highly reducing polyketide synthases (HRPKSs) are an enigmatic group of multidomain enzymes that catalyze the ...
... so it is necessary for these DNA regions to be differentiated from non-promoter sequences. Sequence motif based computational ... In Silico Analysis of Structural Properties of Fungal DNA Sequences and Promoter Prediction Promoters play a central role in ... transcription initiation and gene regulation, so it is necessary for these DNA regions to be differentiated from non-promoter ...
Author(s): Taylor, John W | Abstract: Some fungi with pleomorphic life-cycles still bear two names despite more than 20 years of molecular phylogenetics that have shown how to merge the two systems of classification, the asexual Deuteromycota and the sexual Eumycota. Mycologists have begun to flout nomenclatorial regulations and use just one name for one fungus. The International Code of Botanical Nomenclature (ICBN) must change to accommodate current practice or become irrelevant. The fundamental difference in the size of fungi and plants had a role in the origin of dual nomenclature and continues to hinder the development of an ICBN that fully accommodates microscopic fungi. A nomenclatorial crisis also looms due to environmental sequencing, which suggests that most fungi will have to be named without a physical specimen. Mycology may need to break from the ICBN and create a MycoCode to account for fungi known only from environmental nucleic acid sequence (i.e. ENAS fungi).
2008) Analysis of fungal flora in indoor dust by ribosomal DNA sequence analysis, quantitative PCR, and culture. Appl Environ ... and DNA was eluted with 50 μL of 1× TE (10 mM Tris pH 8, 1 mM EDTA). DNA concentration was quantified on a NanoDrop ... or use have no significant effect on fungal composition. Fungal composition in vastly different types of buildings was most ... Indoor fungal composition is geographically patterned and more diverse in temperate zones than in the tropics. Anthony S. Amend ...
Rapid Detection of Fungal Keratitis with DNA-Stabilizing FTA Filter Paper Nardine Menassa; Philipp P. Bosshard; Claude Kaufmann ... Rapid Detection of Fungal Keratitis with DNA-Stabilizing FTA Filter Paper You will receive an email whenever this article is ... Because of the high retrieval rate of fungal DNA, the analytical sensitivity of the PCR assay was high and comparable to that ... Loffler J Hebart H Schumacher U Reitze H Einsele H . Comparison of different methods for extraction of DNA of fungal pathogens ...
  • Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood. (asm.org)
  • In clinical evaluations, pathogens of more than 200 bacterial and 65 fungal genera have been identified so far. (bionity.com)
  • Here we report the development of DNA microarray for the identification of pathogens causing bloodstream infections. (elsevier.com)
  • This DNA microarray-based pathogen diagnosis takes approximately 10 h starting from a positive blood culture, considerably reducing time required to sufficiently identify pathogens by subsequent agar-culture and biochemical tests which requires altogether at least 1-3 days. (elsevier.com)
  • Thus, the DNA microarray reported here should be useful for the effective identification of microbial pathogens in blood cultures from septicemic patients. (elsevier.com)
  • The article, "Identification of diverse mycoviruses through metatranscriptomics characterization of the viromes of five major fungal plant pathogens," is published in the Journal of Virology . (phys.org)
  • The aim of this study was to assess diversity and composition of fungal communities in living needles of P. heldreichii specifically focusing on fungal pathogens. (mdpi.com)
  • Among all functional groups of fungi, pathogens appeared to be an important component of fungal communities in the phyllosphere of P. heldreichii , especially in those trees under strong abiotic and biotic stress. (mdpi.com)
  • These two genes may belong to a class of proteins specific for filamentous fungi and function as novel virulence factors in fungal pathogens. (plantcell.org)
  • The Sponsor hypothesize that Next-generation Sequencing (NGS) can identify fungal pathogens, is more accurate than other noninvasive options and can report fungal genomic load. (clinicaltrials.gov)
  • The Sponsor aims to describe the incidence of positivity for fungal specific pathogens on NGS analysis in a high-risk immunocompromised pediatric population. (clinicaltrials.gov)
  • We aim to describe the incidence of positivity for fungal specific pathogens on NGS analysis in a high-risk immunocompromised pediatric population. (clinicaltrials.gov)
  • The whole of creation was then subjected to a negative turn of events, including the emergence of fungal pathogens that cause death and disease in plants and animals. (icr.org)
  • Recently, several new studies were published showing how fungal pathogens adapted to their hosts through large-scale genomic changes involving a type of genome degradation marked by gene losses. (icr.org)
  • However, how the DNA damage response pathway is regulated for maintaining the genome integrity of fungal pathogens and how this contributes to their pathogenicity remain elusive. (elsevier.com)
  • Isolation of high quality DNA from cotton and its fungal pathogens. (edu.pl)
  • Thermally dimorphic fungal pathogens, including Histoplasma capsulatum, are a class of soil fungi that undergo a dramatic change in cell shape and virulence gene expression in response to host temperature. (omictools.com)
  • As there are a lot of antibacterial and anti-fungal resistant pathogens, researchers attempt to substitute antimicrobial drugs with various medical plants and novel nanoparticles . (bvsalud.org)
  • Plant diseases, especially those caused by fungal pathogens, jeopardize global crop biosecurity and preventing them requires rapid detection and identification of causal agents. (phys.org)
  • The presence of DNA from pathogens in the Aspergillus genus was detected using qPCR targeting fungal 18S rRNA gene. (biomedcentral.com)
  • Genomic DNA isolated from fungi is appropriate for PCR* and other molecular biology applications. (atcc.org)
  • Fungal DNA barcoding is the process of identifying species of the biological kingdom Fungi through the amplification and sequencing of specific DNA sequences and their comparison with sequences deposited in a DNA barcode database such as the ISHAM reference database, or the Barcode of Life Data System (BOLD). (wikipedia.org)
  • In this attempt, DNA barcoding relies on universal genes that are ideally present in all fungi with the same degree of sequence variation. (wikipedia.org)
  • Fungal DNA barcoding can help to identify and associate anamorphic and teleomorphic stages of fungi, and through that to reduce the confusing multitude of fungus names. (wikipedia.org)
  • The success of identification of fungi by means of DNA barcode sequences stands and falls with the quantitative (completeness) and qualitative (level of identification) aspect of the reference database. (wikipedia.org)
  • 2012) proposed the ITS region as primary DNA barcode region for the genetic identification of fungi. (wikipedia.org)
  • There are other advantages to anther smut as well: the fungal models are safe for researchers to use, the fungus is easy to grow and, unlike humans, fungi have small genomes that make the sequencing process simple. (amherst.edu)
  • The dimorphic probe hybridized with DNA from H . capsulatum , B . dermatitidis , C . immitis , P . brasiliensis , and P . marneffei but not with DNA from nondimorphic fungi. (asm.org)
  • The fungi isolated from the contents of lacrimal sac were identified by DNA sequencing as Wickerhamomyces anamalus (Pichia anomala-Candida pelliculosa) and Galactomyces geotrichum (Geotrichum candidum). (nih.gov)
  • In order to speed up and standardize polymerase chain reaction (PCR)-based detection of medically important fungi in clinical samples we established a combination of commercially available kits for DNA extraction, PCR amplification and detection of the amplicons. (nih.gov)
  • DNA was extracted from 100 mg samples from each of the following fungi: lane 1 from Ganoderma lucidum , lane 2 from Cordyceps sinensis , lane 3 from Agaricus campestris , and lane 4 from Russula foetens . (omegabiotek.com)
  • Purified genomic DNA from various fungi was isolated with the E.Z.N.A.® HP Fungal DNA Kit. (omegabiotek.com)
  • In general, increased exposure to f-DNA was positively related to higher prevalence of all symptoms/diseases (in a dose dependent manner), and significantly related to a decrement of pulmonary function independent from type of fungi. (aspergillus.org.uk)
  • The distribution of the DNA concentration in the wooden disc could possibly be due to where the fungi get in contact and colonize the log. (slu.se)
  • 7 - 9 On the other hand, the clinical diagnosis of fungal keratitis correctly predicts fungi in only 38% of microbiologically proven cases. (arvojournals.org)
  • Here we present a global survey of fungi from indoor environments ( n = 72), using both taxonomic and phylogeny-informative molecular markers to determine whether global or local indoor factors determine indoor fungal composition. (pnas.org)
  • By combining both media approaches to the cultivation of fungi from contaminated and non-contaminated soils, 91 fungal species were recovered, including 63 unidentified species. (frontiersin.org)
  • Introduction The utilization of fungal biodegradation involves the controlled usage of these specially cultivated fungi to treat contaminants. (environmental-expert.com)
  • The first step in performing molecular diagnostics on wood-decay fungi is to obtain analyzable fungal DNA. (environmental-expert.com)
  • They are present in plants, bacteria, various vertebrates, and fungi and were originally considered as sensory photoreceptors because of their incapability to repair cyclobutane pyrimidine dimer (CPD) lesions in duplex DNA. (openaire.eu)
  • The team extracted genetic material, DNA and RNA, from five major types of plant-pathogenic fungi and used computers to search for genetic sequences that resembled those of known viruses. (phys.org)
  • Whereas DNA barcoding via the nuclear ribosomal internal transcribed spacer ITS provides a robust and rapid tool for fungal species delineation, accurate classification of fungi is often arduous given the large number of partial or unknown barcodes and misidentified isolates deposited in public databases. (duhnnae.com)
  • The extraction methods differed in terms of their ability to efficiently extract DNA from particular species of fungi (e.g. (cdc.gov)
  • To prevent the spread of fungi and its harmful effects, try using an anti-fungal spray like MesoSilver. (ehow.com)
  • In the current research, silver, zinc, and gold nanoparticles were used to increase the yield of DNA for two plant pathogenic fungi including soil-borne fungus Rhizoctonia solani and toxigenic fungus Alternaria alternata. (edu.pl)
  • Fungi were identified by culture and polymerase chain reaction, which revealed high sequence homology to six different fungal species. (biomedsearch.com)
  • Fungal Diversity Survey, or FunDiS, is a nonprofit citizen science organization formerly known as North American Mycoflora Project, Inc. FunDiS aims to document the diversity and distribution of fungi across North America "in order to increase awareness of their critical role in the health of ecosystems and allow us to better protect them in a world of rapid climate change and habitat loss. (wikipedia.org)
  • The project encourages amateurs, working with professionals, to contribute observations to online databases vetted by experts, and to collect and document fungi for DNA barcoding. (wikipedia.org)
  • In August 2020 the organization changed its name to Fungal Diversity Survey to reflect the fact that "fungi are their own kingdom - as long as they get lumped in with plants they will not get the recognition, attention and protection they deserve. (wikipedia.org)
  • Diagnosis of fungal infections is typically made by isolation of the infecting organism in culture, by serologic assays, or through histopathologic examination of tissue ( 13 , 27 ). (asm.org)
  • Histopathologic diagnosis of fungal infections is typically made through morphological criteria using reagents that preferentially stain fungal structures ( 6 ). (asm.org)
  • There were positive associations between levels of Aspergillus versicolor DNA and daytime breathlessness, between C14 3-OH and respiratory infections and between ergosterol and doctors diagnosed asthma. (ersjournals.com)
  • However, the recent rise in fungal infections caused by species formerly considered benign but now seen as causing disease in immunosuppressed humans, and a vastly increased resolution of indoor fungal composition afforded by culture-independent sampling methods ( 11 , 12 ), force us to reconsider what constitutes a normal indoor environment and the factors that shape it. (pnas.org)
  • The article was written shortly after the peak of the fungal meningitis outbreak caused by contaminated injections that resulted in dozens of deaths and hundreds of infections. (environmental-expert.com)
  • All relevant materials for patients and clinicians concerning the multistate outbreak of fungal meningitis and other infections are located on this page. (cdc.gov)
  • This relapse incidence is lower than the prevalence of relapse observed for other invasive fungal infections. (cdc.gov)
  • Important memory T cells that may protect patients from fungal and viral infections are also removed in the T depletion process. (clinicaltrials.gov)
  • Problems with this system may lead to frequent, severe, or unusual fungal infections. (clinicaltrials.gov)
  • Researchers want to collect blood and tissue samples from people who have unusual, persistent or severe fungal infections or immune problems that increase the risk of these infections. (clinicaltrials.gov)
  • To collect medical information and samples for a long-term study of people with immune system problems that lead to fungal infections. (clinicaltrials.gov)
  • People with a history of fungal infections caused by immune system problems. (clinicaltrials.gov)
  • This protocol is a natural history study designed to investigate the clinical, microbiologic, genetic and immunologic correlates of primary immune deficiencies and other conditions associated with mucocutaneous and invasive fungal infections (IFIs). (clinicaltrials.gov)
  • Zygomycosis is the third most common invasive fungal infection, representing 8.3 to 13 percent of fungal infections at autopsy in hematology patients. (cap.org)
  • In this study, we investigated the DNA damage response pathway in the basidiomycete pathogen Cryptococcus neoformans, which causes life-threatening meningoencephalitis in immunocompro-mised individuals, with an average of 223,100 infections leading to 181,100 deaths reported annually. (elsevier.com)
  • A new study from The Westmead Institute for Medical Research has uncovered how serious fungal infections grow in humans by conserving phosphate, highlighting a possible target for treatment. (medicalxpress.com)
  • The serum CrAg-latex agglutination the fungal infections and preserve engraftment. (cdc.gov)
  • What broad spectrum anti-fungal drug is indicated for treatment of severe to life-threatening fungal infections? (brainscape.com)
  • Which Triazolole is a broad spectrum drug that is indicated for systemic fungal infections, as well as oral and esophageal candidiasis? (brainscape.com)
  • The interspecific variation, i.e., the variation between species, in the chosen DNA barcode gene should exceed the intraspecific (within-species) variation. (wikipedia.org)
  • Moreover, fungal species can comprise multiple strains that can vary in their morphology or in traits such as carbon- and nitrogen utilisation, which has often led to their description as different species, eventually producing long lists of synonyms. (wikipedia.org)
  • To find out, the authors used a genetic technique called DNA barcoding to quickly estimate that another 11 unnamed or "candidate" species were also present. (innovations-report.com)
  • In DNA barcoding, short genetic sequences that uniquely identify known species are generated and stored in public databases. (innovations-report.com)
  • Our DNA barcode data reveal new species even at this relatively well-studied site, yet the field sampling shows that many of these species new to science are already gone here. (innovations-report.com)
  • E.Z.N.A.® Fungal DNA Mini Kit allows for the rapid and reliable isolation of high-quality total cellular DNA from a wide variety of fungal species and tissues. (omegabiotek.com)
  • The fungal kingdom is a large group of eukaryotic organisms consisting of more than 100 000 known and an expected number of more than 1 million species with a wide variety of lifestyles. (slu.se)
  • Mould surveys that target the relatively few visibly apparent fungal species or those readily cultivable on artificial media are now standard, and a US Environmental Protection Agency-developed set of real-time PCR probes facilitates their quantification ( 10 ). (pnas.org)
  • The fungal gut communities of most worker bees were highly dominated by Saccharomyces but foraging bees and queens were colonised by diverse fungal species and Zygosaccharomyces , respectively. (nature.com)
  • In comparison with FEs in agricultural grasses, research on endophytes in woody plants has been relatively sparse ( 14 ), attributable, in part, to much higher fungal species heterogeneity within and among hosts ( 24 ⇓ - 26 ). (pnas.org)
  • In this study, like the yet-to-be cultured bacteria, high concentrations of nutrients selected for predominantly different fungal species to that recovered using a low nutrient media. (frontiersin.org)
  • A preliminary biodegradation activity assay on a selection of isolates found that a high proportion of novel and described fungal species from a range of soil samples were capable of hydrocarbon degradation and should be characterized further. (frontiersin.org)
  • Given the biotechnological potential of fungal enzymes there is a need to increase efforts for greater species recovery. (frontiersin.org)
  • 1. INTRODUCTION Strategically eliminating damage caused by fungus requires precise identification of the fungal species present (using either conventional or molecular diagnostic techniques). (environmental-expert.com)
  • Traditional methods of identifying fungal exposures in occupational environments, such as culture and microscopy-based approaches, have several limitations that have resulted in the exclusion of many species. (cdc.gov)
  • ITS sequencing results in the detection of many fungal species that are either not detected or difficult to identify to species level using culture or microscopy. (cdc.gov)
  • While these methods do not provide quantitative measures of fungal burden, they offer a new approach to hazard identification and can be used to determine overall species richness and diversity within an occupational environment. (cdc.gov)
  • The species composition varied greatly between ITS clone libraries generated with the different DNA extraction kits. (cdc.gov)
  • However, compared to viable culture data, ITS clone libraries included additional fungal species that are incapable of growth on solid culture medium. (cdc.gov)
  • Collectively, our data indicated that DNA extraction methodologies used in ITS sequencing studies of occupational or environmental dust samples can greatly influence the fungal species that are detected. (cdc.gov)
  • Environmental DNA (eDNA) sampling is a powerful tool for detecting invasive and native aquatic species. (usda.gov)
  • Taxonomy of the Gomphales has been revisited by combining morphology and molecular data (DNA sequences) to provide a natural classification for the species of Gomphus sensu lato. (usda.gov)
  • To facilitate the curation and functional and evolutionary studies of CYPs, we previously developed Fungal Cytochrome P450 Database (FCPD), which included CYPs from 70 fungal and oomycete species. (biomedcentral.com)
  • FCPD version 1.2 provides a systematic and searchable catalogue of 9,550 fungal CYP sequences (292 families) encoded by 108 fungal species and 147 CYP sequences (9 families) encoded by five oomycete species. (biomedcentral.com)
  • Denaturing gradient gel electrophoresis showed that fungal communities on the PU were less diverse than in the soil, and only a few species in the PU communities were detectable in the soil, indicating that only a small subset of the soil fungal communities colonized the PU. (asm.org)
  • We analyzed DNA sequence data from various boreal and arctic macrofungi using phylogenetic and coalescent methods to assess the genetic diversity at the species and intraspecific levels. (nps.gov)
  • Fungal Conservation - A Conservation Working Group of experts identifies species of concern and their habitats, develops regional watchlists, and engages amateur citizen scientists to look for and document target specimens. (wikipedia.org)
  • Molzym announces CE IVD marking of their robotic microbial DNA isolation and direct PCR test, Micro-Dx™, for routine pathogen diagnosis. (bionity.com)
  • The generalist fungal pathogen Pyrenophora semeniperda occurs primarily in cheatgrass (Bromus tectorum) seed banks, where it causes high mortality. (usda.gov)
  • In the first study, scientists examined the genome of the obligate biotroph Pneumocystis jirovecii , a fungal pathogen found in human lungs. (icr.org)
  • Like the fungal pathogen in the first study, it is also a biotroph. (icr.org)
  • Cryptococcus neoformans is a fungal pathogen that infects people with weakened immune systems, particularly those with advanced HIV/AIDS. (medicalxpress.com)
  • The region is flanked by the DNA sequences for the ribosomal small subunit (SSU) or 18S subunit at the 5' end, and by the large subunit (LSU) or 28S subunit at the 3' end. (wikipedia.org)
  • This is the first time that we've used genetic barcodes-DNA sequences unique to each living organism-to characterize an entire amphibian community," said Eldredge Bermingham, STRI director and co-author. (innovations-report.com)
  • Dust was vacuumed from 32 classrooms and analysed for different endotoxin as 3-hydroxy fatty acids (3-OH), muramic acid, ergosterol, allergens and five fungal DNA sequences. (ersjournals.com)
  • Five sequences of fungal DNA were analyzed by quantitative PCR. (diva-portal.org)
  • Promoters play a central role in transcription initiation and gene regulation, so it is necessary for these DNA regions to be differentiated from non-promoter sequences. (jbsdonline.com)
  • We found a lot of sequences that were very similar to previously described fungal viruses, but we also found some encapsidated forms that were similar to plant viruses. (phys.org)
  • We have devised a new strategy to clone DNA sequences from an yeast autonomously-propagating plasmid into a non-autonomous integrative vector by in-vivo recombination. (nih.gov)
  • Updated DNA Sequences ===================== AC007505 AC007505 134499bp DNA PLN 09-OCT-1999 Arabidopsis thaliana chromosome I BAC F28L22 genomic sequence, complete sequence. (bio.net)
  • GenBank from the NCBI is an amazing and invaluable resource for DNA sequences, and combined with the searching tool BLAST it is really easy to identify your unknown gene sequence. (rhizobia.co.nz)
  • Nucleotide sequences of the de-sired portions (ITS and LSU rDNA) of the DNA samples were obtained using standard molecular protocols (DNA extraction, polymerase chain reaction, cycle sequencing etc. (nps.gov)
  • The N-terminal region of a number of fungal transcriptional regulatory proteins contains a Cys-rich motif that is involved in zinc-dependent binding of DNA. (ebi.ac.uk)
  • Viral DNA-binding proteins have served as good models to study the biochemistry of transcription regulation and chromatin dynamics. (biomedcentral.com)
  • Computational analysis of viral DNA-binding regulatory proteins and identification of their previously undetected homologs encoded by cellular genomes might lead to a better understanding of their function and evolution in both viral and cellular systems. (biomedcentral.com)
  • The phyletic range and the conserved DNA-binding domains of the viral regulatory proteins of the poxvirus D6R/N1R and baculoviral Bro protein families have not been previously defined. (biomedcentral.com)
  • Using computational analysis, we show that the amino-terminal module of the D6R/N1R proteins defines a novel, conserved DNA-binding domain (the KilA-N domain) that is found in a wide range of proteins of large bacterial and eukaryotic DNA viruses. (biomedcentral.com)
  • The amino-terminal module of the Bro proteins is another, distinct DNA-binding domain (the Bro-N domain) that is present in proteins whose domain architectures parallel those of the KilA-N domain-containing proteins. (biomedcentral.com)
  • We define a large class of novel viral DNA-binding proteins and their cellular homologs and identify their domain architectures. (biomedcentral.com)
  • Much less is known of the domain architecture and evolutionary history of those viral DNA-binding regulatory proteins whose cellular homologs have not (yet) been identified. (biomedcentral.com)
  • Proteins obviously play critical roles in diverse biological processes through interactions with other proteins, DNA, RNA, and small molecules both inside and outside cells. (genetics.org)
  • We dissect the circuit driven by three regulators that control yeast-phase growth, and demonstrate that these factors, including two deeply conserved Velvet family proteins of unknown function, associate with DNA. (omictools.com)
  • Cytochrome P450 proteins (CYPs) play diverse and pivotal roles in fungal metabolism and adaptation to specific ecological niches. (biomedcentral.com)
  • Here we use an Illumina-based amplicon sequencing to investigate bacterial and fungal communities associated with spontaneous coffee-bean fermentation in Colombia. (nature.com)
  • Bacterial and fungal communities in vineyard soils were shaped by both time course and soil management (i.e., the use of biostimulants and irrigation). (mdpi.com)
  • Edaphic factors rarely influence bacterial and fungal communities. (mdpi.com)
  • Changes in fermentation characteristics were determined, and the composition of bacterial and fungal communities were assessed using metagenomic sequencing. (asm.org)
  • Fulton PJ, Chungwongse J, Tanksley SS (1995) Microprep protocol for extraction of DNA from tomato and other herbaceous plants. (springer.com)
  • Five commercially available extraction kits and an in-house DNA extraction method for the release of DNA from Candida albicans and Aspergillus niger cells were assessed for sensitivity, purity, duration, and cost. (asm.org)
  • All commercially available kits helped to shorten the duration of DNA extraction. (asm.org)
  • SepsiTest-UMD CE IVD includes all reagents and consumables necessary for DNA extraction and broad range PCR or Real-Time PCR analysis as well as primers for Sanger-sequencing. (bionity.com)
  • However, techniques of specimen collection and DNA extraction before PCR may interfere with test sensitivity. (arvojournals.org)
  • Whatman International, Ltd., Maidstone, UK) for specimen collection without DNA extraction in a single-step, nonnested PCR for fungal keratitis. (arvojournals.org)
  • Bacterial DNA Barcoding PCR Kit, without nucleic acid extraction. (base-asia.com)
  • Fungal DNA Barcoding PCR Kit, without nucleic acid extraction. (base-asia.com)
  • Extraction reagent is included for Bacterial DNA Barcoding Kit only. (base-asia.com)
  • Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA. (cdc.gov)
  • This protocol details an approach for determining fungal diversity within air samples through genomic DNA extraction, amplification, sequencing, and taxonomic identification of fungal internal transcribed spacer (ITS) regions. (cdc.gov)
  • Three commonly used DNA extraction methodologies (UltraClean Soil kit, High Pure PCR Template kit, and EluQuik/DNeasy kit) were compared in terms of sensitivity and susceptibility to PCR inhibitors in dust for three common fungal bioaerosols, Aspergillus versicolor, Rhizopus microsporus and Wallemia sebi. (cdc.gov)
  • In addition, the ability to remove PCR inhibitors from dust samples was most effective using the soil DNA extraction kit. (cdc.gov)
  • Efficiency of the different DNA extraction methods. (cdc.gov)
  • A. Genomic DNA was isolated from different concentrations of spores using three different DNA extraction methods. (cdc.gov)
  • Lanes marked with '0' represent negative controls in which the input for DNA extraction was water containing no spores. (cdc.gov)
  • C. qPCR standard curves of Aspergillus versicolor genomic DNA isolated with each of the three DNA extraction methods. (cdc.gov)
  • Removal of PCR inhibitors by the different DNA extraction methods tested. (cdc.gov)
  • MPure Viral Nucleic Acid Extraction Kit 800 is used with the MPure-12 instrument for extraction of Viral DNA or RNA from human biological specimens such as serum, plasma, and other cell-free fluids. (mpbio.com)
  • Gold nanoparticles combined with zinc and silver nanoparticles enhanced both DNA yield and PCR products compared to DNA extraction methods with ALB buffer, sodium dodecyl sulfate, ALBfree from protinase K, ZnNPs and AgNPs. (edu.pl)
  • Human 18S rRNA gene qPCR confirmed successful DNA extraction of all samples. (biomedcentral.com)
  • Its hard to say how bad/good your results are to be honest and: smears are in general not that that weird to have when doing a genomic DNA extraction. (protocol-online.org)
  • DNA extraction , buffer solution and 2 more. (protocol-online.org)
  • DNA extraction with phenol-chloroform - DNA stuck in phenol layer? (protocol-online.org)
  • microbiome , DNA extraction and 3 more. (protocol-online.org)
  • PCR , cPCR , DNA extraction and 1 more. (protocol-online.org)
  • These data demonstrate that the variation in the occurrence of the 1.2 Mb minichromosome did not arise by rearrangement of the genome of a progenitor strain but involved either large scale deletion or addition of DNA. (nih.gov)
  • It is being said that the modern human genome sequence may carry a leftover contribution from Neanderthal DNA. (i4u.com)
  • Moreover, in a selected group of patients, all patients with proven and probable invasive fungal infection were found to be PCR-positive. (nih.gov)
  • The following update provides information on clinical follow-up of patients affected in the unprecedented multistate invasive fungal infection outbreak associated with contaminated methylprednisolone injections in late 2012 and 2013. (cdc.gov)
  • Changes observable on MRI consistent with a fungal infection have proven valuable in the identification of cases, especially in patients with a history of significant baseline pain. (cdc.gov)
  • The procedures for each person may vary with the particular diagnosis and the extent of fungal infection. (clinicaltrials.gov)
  • For inclusion, patients must have a history of or an active mucocutaneous or invasive fungal infection, but may or may not have a defined primary or acquired immunodeficiency state. (clinicaltrials.gov)
  • A better understanding of primary immunodeficiency and identification of fungal and host risk factors for fungal infection may provide new insights into pathogenesis and identify targets for development of novel therapies. (clinicaltrials.gov)
  • Follow-up may occur every 6 months or more frequently depending on clinical course, the underlying risk factor(s), and the type of fungal infection. (clinicaltrials.gov)
  • Under some circumstances, standard medical treatment will be provided for a fungal infection or immune deficiency. (clinicaltrials.gov)
  • It also highlights the utility of gene sequencing in a postmortem setting for speciation of fungal organisms, both for providing a definitive diagnosis and additional information for infection control. (cap.org)
  • ii) or a prodrug thereof, sufficient to inhibit or reduce fungal infection or fungal growth. (freepatentsonline.com)
  • Histopathology of microbiological remission of the infection, dos- the graft showed organisms consistent with Crypto- age was adjusted on the basis of renal function coccus yeast cells, suggesting fungal pyelonephritis. (cdc.gov)
  • We test these hypotheses ( i ) by examining whether the global distribution patterns of individual taxa show range limitation, ( ii ) by examining whether indoor fungal composition correlates with dispersal limitation or outdoor environment, and ( iii ) by testing an alternative hypothesis that indoor environment determines fungal composition by comparing nearby buildings with different architecture and function. (pnas.org)
  • Sequencing resulted in 31,831 high quality reads, which after assembly were found to represent 375 fungal taxa. (mdpi.com)
  • Our results suggest that Beringia, particularly Alaska, harbors very diverse fungal communities and that most arctic and at least some boreal fungal taxa survived the last glacial maximum in Beringia. (nps.gov)
  • Fungal contamination, especially of Aspergillus versicolor, can be risk factors. (ersjournals.com)
  • The present study was conducted to characterize antimicrobial activities of Euphorbia prostrata and Pelargonium graveolens extract alone and in combination with [email protected] & Mn Fe (OH)3-NPs on the DNA cleavage of E. coli and also Bacillus subtilis , Pseudomonas aeruginosa , Escherichia coli , Staphylococcus aureus , Aspergillus oryzae , and Candida albicans . (bvsalud.org)
  • Detection of Aspergillus DNA in BAL fluid by quantitative PCR (qPCR) offers the potential for earlier diagnosis and higher sensitivity. (biomedcentral.com)
  • Receiver-operating characteristic analysis of qPCR showed that a cutoff of 13 fg of Aspergillus genomic DNA generated a sensitivity, specificity, positive and negative predictive value of 77%, 88%, 50%, 96% respectively. (biomedcentral.com)
  • Some patients did not meet standard criteria for IPA, but had consistently high levels of Aspergillus DNA in BAL fluid by qPCR. (biomedcentral.com)
  • The Aspergillus qPCR assay detected Aspergillus DNA in 76.9% of subjects with proven or probable IPA when the concentrated BAL fluid pellet fraction was used for diagnosis. (biomedcentral.com)
  • Our study focuses on developing a quantitative PCR (qPCR) platform to detect Aspergillus DNA in BAL as an indicator of IPA. (biomedcentral.com)
  • Sequencing of a high number of fungal genomes has become possible due to the development of next generation sequencing techniques (NGS). (springer.com)
  • It's a difficult question to explore since the answer involves tracking human DNA and the harmful mutations genomes accumulate over long periods of time. (amherst.edu)
  • A detailed analysis of the KilA-N and Bro-N domains and the associated domains points to extensive domain shuffling and lineage-specific gene family expansion within DNA virus genomes. (biomedcentral.com)
  • The comparative analysis of these genomes relative to one another and to other sequenced fungal genomes is provided here. (plos.org)
  • Fungal genomes encode extremely variable "CYPomes" ranging from one to more than 300 CYPs. (biomedcentral.com)
  • Despite the rapid growth of sequenced fungal and oomycete genomes and the resulting influx of predicted CYPs, the vast majority of CYPs remain functionally uncharacterized. (biomedcentral.com)
  • These features will facilitate functional annotation and classification of CYPs encoded by newly sequenced fungal and oomycete genomes. (biomedcentral.com)
  • It's a question that has baffled scientists since they began understanding DNA, and is one that Michael E. Hood, associate professor of biology, believes he can help answer-using a violet fungus that infects a tiny carnation-like flower. (amherst.edu)
  • On the basis of phyletic pattern analysis we present evidence for a probable viral origin of the fungus-specific cell-cycle regulatory transcription factors containing the APSES DNA-binding domain. (biomedcentral.com)
  • One Fungus = One Name: DNA and fungal nomenclature twenty years after PCR. (escholarship.org)
  • Those were the ones we were most interested in, because they reduce fungal virulence and can be transmitted outside the fungus," he says. (phys.org)
  • Rice blast, which is caused by this heterothallic haploid fungus, is one of the most severe fungal diseases of rice throughout the world ( Valent, 1990 ). (plantcell.org)
  • In the filamentous fungus Neurospora crassa , genes unpaired during meiosis are silenced by a process known as meiotic silencing by unpaired DNA (MSUD). (g3journal.org)
  • We previously reported that a unique transcription factor, Bdr1, whose expression is strongly induced by the protein kinase Rad53 governs DNA damage responses by controlling the expression of DNA repair genes in the basidiomycetous fungus Cryptococcus neoformans. (elsevier.com)
  • Expression of the linear DNA plasmid pRS64 in the plant pathogenic fungus Rhizoctonia solani' Mol.Gen.Genet.245. (nii.ac.jp)
  • The complete nucleotide sequence and characterizatior of the linear DNA plasmids pRS64 from the plant pathogenic fungus Rhizoctonia solani' Curr.Genet. (nii.ac.jp)
  • I am trying to extract Dna from fungus (Alternaria alternata). (protocol-online.org)
  • Following DNA isolation, it was amplified using ITS2 rDNA as a marker and subjected to high-throughput sequencing. (mdpi.com)
  • Rapid method for the detection of Ralstonia solanacearum by isolation DNA from infested potato tubers based on magnetic nanotools. (edu.pl)
  • A gene library was prepared specifically from the 1.2 Mb minichromosome and ten independent DNA clones unique to this chromosome were identified by differential hybridisation to whole chromosome probes. (nih.gov)
  • Using pyrosequencing of the 16S rRNA gene and ITS2 region amplicons, we analysed the bacterial and fungal gut communities of the honey bee as affected by the host social status. (nature.com)
  • 1st BASE's Bacterial and Fungal DNA Barcoding Kits are designed to amplify conserved region of bacterial 16s rRNA gene and fungal ITS gene respectively for Sanger sequencing. (base-asia.com)
  • 100 years ago (set as the control) to explore the impact of wildfire on the soil chemistry, soil microbial stoichiometry, and the fungal-to-bacterial gene ratio (F:B ratio). (springer.com)
  • Wildfire-induced reduction in vegetation biomass positively affected the fungal, but not the bacterial, gene copy number. (springer.com)
  • The method consists of a first step in which the replicative plasmid carrying the DNA fragment of interest forms a co-integrate with the non-replicative plasmid by an induced in-vivo reciprocal exchange accompanied by gene conversion. (nih.gov)
  • DNA methyltransferase, motifs IX-X. AF139372 AF139372 2115bp DNA PLN 10-OCT-1999 Arabidopsis thaliana DNA methyltransferase (METI) gene, partial cds. (bio.net)
  • Although much of the extraordinary diversity of the pan-fungal CYPome can be attributed to gene duplication and adaptive divergence, our analysis also suggests a few potential horizontal gene transfer events. (biomedcentral.com)
  • he ITS gene region has been selected as the official gene for fungal barcoding in a recent publication of the Proceedings of the National Academy of Sciences. (rhizobia.co.nz)
  • Furthermore, deletion of both RAD53 and CHK1 increased susceptibility to certain antifungal drugs such as amphotericin B. This report provides insight into the regulatory mechanism of fungal DNA damage repair systems and their functional relationship with fungal virulence and antifungal drug susceptibility. (elsevier.com)
  • Here, we found that perturbation of Rad53-and Chk1-dependent DNA damage response pathways attenuated the virulence of C. neoformans and increased its susceptibility to certain antifungal drugs, such as amphotericin B and flucytosine. (elsevier.com)
  • B. Genomic DNA was isolated from different concentrations of a heterogeneous spore mixtures (containing a 1:1:0.25 ratio of A. versicolor: W. sebi: R. microsporus spores). (cdc.gov)
  • DNA was isolated from 10,000 spores spiked with dust. (cdc.gov)
  • According to Science Daily, your pillow may contain millions of fungal spores. (ehow.com)
  • These fungal spores can lead to numerous respiratory issues, as well as exacerbate preexisting conditions such as asthma or allergies. (ehow.com)
  • Hierarchical clustering analysis (HCA) allowed us to define five major clusters, significantly associated with fungal load ( p = 0.029), in which copy number of mitochondrial genes was significantly different among them. (frontiersin.org)
  • The recent utilization of molecular methods such as internal transcribed spacer (ITS) sequencing of ribosomal RNA genes has provided improved insight into the diversity of fungal bioaerosols in indoor, outdoor and occupational environments. (cdc.gov)
  • Transcriptome analysis revealed that Rad53 regulates the expression of several DNA repair genes in response to gamma radiation. (elsevier.com)
  • Fungal diseases account for approximately 10 percent yield losses annually in corn and soybean. (phys.org)
  • Interestingly, the research could also improve medical treatment options for human fungal diseases . (phys.org)
  • Aquatic organisms in marine systems and freshwaters are threatened by fungal and fungal-like diseases globally. (phys.org)
  • Therefore, histopathologic examination of tissue sections may be the most rapid or only way in which to diagnose invasive fungal disease. (asm.org)
  • Future Market Insights offers an in-depth assessment of the global anti-microbial/anti-fungal tests market for the period 2017 to 2027. (pharmiweb.com)
  • The report titled 'Anti-microbial/Anti-fungal Tests Market: Global Industry Analysis 2012-2016 and Opportunity Assessment 2017-2027' is a comprehensive study of the anti-microbial/anti-fungal tests market across different geographic regions with a global perspective. (pharmiweb.com)
  • FTA filter paper for specimen collection together with direct PCR is a promising method of detecting fungal keratitis. (arvojournals.org)
  • Traditional methods of assessing fungal exposure have been confounded by a number of limiting variables. (cdc.gov)
  • Voucher specimens should be clearly labelled as such, including a permanent voucher identifier that unambiguously connects the specimen with the DNA barcode data derived from it. (wikipedia.org)
  • By comparing DNA profiles from unknown organisms to the databases, researchers can identify biological specimens quickly, and construct genetic lineages. (innovations-report.com)
  • In 2018-2019 NAMP registered 160 projects by amateur mycophiles and conducted DNA barcoding on more than 5,000 fungal specimens. (wikipedia.org)
  • The invention relates to compounds including peptides and peptidomimetics having anti-fungal activity alone, and in combination with other agents that have anti-fungal activity. (freepatentsonline.com)
  • 2. The method of claim 1, wherein the peptide or peptidomimetic comprises (d-Bpa) (d-Ser)(d-Trp)(d-Ser)(d-Phe-2,3,4,5,6-F)(d-Cha), and has anti-fungal activity. (freepatentsonline.com)
  • 18. The method of claim 17, wherein the peptide or peptidomimetic comprises (d-Bpa) (d-Ser)(d-Trp)(d-Ser)(d-Phe-2,3,4,5,6-F)(d-Cha), and has anti-fungal activity. (freepatentsonline.com)
  • If there's nothing to interact with, then there can't be an anti-fungal activity. (brainscape.com)
  • Comparative analysis of fungal and oomycete CYPomes revealed cases of both extreme expansion and contraction. (biomedcentral.com)
  • Chemidlin Prévost-Bouré N, Christen R, Dequiedt S, Mougel C, Lelièvre M, Jolivet C, Shahbazkia HR, Guillou L, Arrouays D, Ranjard L (2011) Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR. (springer.com)
  • Methods: Measurements of fungal DNA (f-DNA) were performed by quantitative polymerase chain reaction (PCR) in classrooms attended by 616 schoolchildren (mean age 10yrs) of Italy, Denmark, Sweden, Norway, and France (EU-funded HESE Study, Health Effects of School Environment). (aspergillus.org.uk)
  • Results The quantitative result showed that total and bacterial DNA accumulated monotonically, rising to 41 ng/cm2 for total DNA and to 2.8 ng/cm2 for bacterial DNA over the 21-week period. (microbe.net)
  • The dip indicated a possible artifact of this sampling approach for quantitative analysis as DNA may not be conserved on the filter over the months-long service period. (microbe.net)
  • Polymerase chain reaction (PCR) is increasingly important for the rapid detection of fungal keratitis. (arvojournals.org)
  • We measured cat, dog and horse allergens and fungal DNA in cabin dust and microbial volatile organic compounds (MVOCs) in cabin air. (diva-portal.org)
  • The removal of human DNA and free-circulating microbial DNA ensures high detection specificity of exclusively DNA of intact cells. (bionity.com)
  • Others have shown temporal fluctuations of fungal assemblages in human environments and modest correlations with human activity, but global-scale patterns have not been examined, despite the manifest significance of biogeography in other microbial systems. (pnas.org)
  • The existence of global-scale patterns in fungal composition, however, is unexamined, despite evidence of biogeographical patterning in other microbial systems. (pnas.org)
  • Microbial network analyses suggested that the bacterial associations were weaker than the fungal ones under half irrigation and that the inoculation with AMF led to the increase in positive associations between vineyard-soil-living microbes. (mdpi.com)
  • The microbial C:N ratios were independent of the shifts in the fungal-to-bacterial ratio when C:N exceeded 12. (springer.com)
  • We conclude that microbial stoichiometry does not always respond to changes in the fungal-to-bacterial ratio and that wildfire-induced permafrost thawing does not accelerate microbial respiration. (springer.com)
  • 2014 ). A higher fungal-to-bacterial (F:B) ratio may, therefore, be associated with a higher C:N ratio for the total microbial biomass. (springer.com)
  • Anti-microbial/Anti-fungal Tests Market Size, Share, Grow. (pharmiweb.com)
  • Leading companies actively involved in the anti-microbial/anti-fungal tests market have been analysed in the report. (pharmiweb.com)
  • The market position and product portfolios have been mentioned to present an accurate picture of their present and future prospects in the anti-microbial/anti-fungal tests market. (pharmiweb.com)
  • The revenues have been aggregated for a valuation of the anti-microbial/anti-fungal tests market for the historical period from 2012 to 2016. (pharmiweb.com)
  • Pathological examination confirmed a diagnosis of fungal dacryocystitis based on the fungal hyphae observed on the excised lacrimal sac wall. (nih.gov)
  • It is an ideal kit to perform DNA barcoding for pure isolates of bacterial and fungal samples readily. (base-asia.com)
  • BLAST searches revealed 26 unknown OTUs and 50 isolates corresponding to previously uncultured, unidentified fungal clones. (duhnnae.com)
  • Detection of transcripts homologous to DNA plasmids in isolates from six anastomosis groups of Rhizoctonia solani' Ann.Phytopathol.Soc.Jpn.62. (nii.ac.jp)
  • In this chapter, we propose an optimized protocol allowing the preparation of high quality and long fragment DNA from different samples (mycelium, fruiting body, soil) compatible with the current sequencing requirements. (springer.com)
  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. (springer.com)
  • Taken together, this work clearly demonstrates the applicability of three dyes for sequencing of DNA at reasonable cost and within acceptable process time managing the influence of crosstalk by mathematical reduction of the respective coefficient. (beck-shop.de)
  • Meanwhile, the internal transcribed spacer (ITS) sequencing by Illumina Miseq platform at each taxonomic level demonstrated that the toxicological effect of oil pollutants obviously influenced the fungal diversity and community structure in soil. (hindawi.com)
  • The Genomic DNA Purification Kit allows DNA to bind to the glass fiber matrix of the genomic DNA spin column. (biovision.com)
  • The E.Z.N.A.® High Performance (HP) Fungal DNA Kit is designed for efficient recovery of genomic DNA from fresh and dried fungal tissue samples rich in polysaccharides or having lower DNA content. (omegabiotek.com)
  • Manter DK, Vivanco JM (2007) Use of the ITS primers, ITS1F and ITS4, to characterize fungal abundance and diversity in mixed-template samples by qPCR and length heterogeneity analysis. (springer.com)
  • In this study, the fungal ITS2 region is amplified with the primers gITS7, ITS4A and ITS4 from samples collected from naturally decaying Norway spruce logs. (slu.se)
  • You are buying fungal DNA primers for use in PCR reactions (specifically ITS and LSU regions. (allthemushrooms.com)
  • Minor cross-reactivity was observed for the B . dermititidis probe used against C . immitis DNA and for the H . capsulatum probe used against Candida albicans DNA. (asm.org)
  • The aim of this protocol is to use modern methods in molecular and cell biology and immunology to elucidate the immunopathogenesis of fungal disease in humans. (clinicaltrials.gov)
  • The method is particularly useful for the cloning of large DNA fragments and can be used for plasmids from organisms other than yeasts. (nih.gov)
  • We report this case for the purpose of recognizing a rapidly fatal process in an immunocompromised patient and to describe the utility of molecular methods in speciation of fungal organisms in a postmortem setting. (cap.org)
  • Living organisms are constantly exposed to DNA damage stress caused by endogenous and exogenous events. (elsevier.com)
  • Therefore, living organisms have evolutionarily conserved signaling networks to sense and repair DNA damage. (elsevier.com)
  • DNA addition or deletion is associated with a major karyotype polymorphism in the fungal phytopathogen Colletotrichum gloeosporioides. (nih.gov)
  • Our main objective is to produce lists of accepted and rejected names in Colletotrichum before the next IMC fungal conference . (rhizobia.co.nz)
  • Queen Mary University of London researchers discovered the genetic sequence of ash tree that can treat fungal disease, ash dieb. (i4u.com)
  • Inhibition in each qPCR was measured using an exogenous DNA based internal amplification control (IAC). (biomedcentral.com)
  • Specific probes for H . capsulatum , B . dermatitidis , C . immitis , P . brasiliensis , P . marneffei , S . schenckii , C . neoformans , and P . carinii hybridized with homologous but not heterologous DNA. (asm.org)
  • The KilA-N domain is suggested to be homologous to the fungal DNA-binding APSES domain. (biomedcentral.com)
  • All student submissions will be considered for awards given out by the Fungal Genetics Conference. (genetics-gsa.org)
  • Fungal Contamination of Paraffin Wax Blocks in a Pathology Archive. (biomedsearch.com)
  • However, the sensitivity varied from 1 to 1,000 fungal cells/ml and costs varied from $0.10 to 2.30. (asm.org)
  • The QIAmp Tissue kit was the commercially available assay that yielded the same sensitivity and purity of fungal DNA release as the in-house protocol but was the most expensive method. (asm.org)
  • Also, by using ZnNPs and AgNPs the DNA yield was enhanced and the sensitivity of random amplified polymorphic DNA (RAPD) PCR products was increased. (edu.pl)
  • Culture and histopathological examination of bronchoalveolar lavage (BAL) fluid are useful but have suboptimal sensitivity and in the case of culture may require several days for fungal growth to be evident. (biomedcentral.com)
  • This preparation of high molecular weight DNA is appropriate for the use in the polymerase chain reaction (PCR)* process and other molecular biology applications. (atcc.org)
  • Advances in the field over the last two decades have led occupational health researchers to turn to molecular-based approaches for identifying fungal hazards. (cdc.gov)
  • After 5 months of burial, the fungal communities on the surface of the PU were compared with the native soil communities using culture-based and molecular techniques. (asm.org)
  • As expected on the basis of yeast two-hybrid and co-immunoprecipitation (Co-IP) experiments, the Neurospora DNA methyltransferase DIM-2 was found in a complex with HP1. (genetics.org)
  • These characteristics are independent of whether the DNA insert is flanked by appropriate restriction sites or whether it does, or does not, express a detectable phenotype in yeast. (nih.gov)
  • The system combines the reversible nucleic acid-binding properties of Omega's HiBind® matrix with the speed and versatility of spin columns to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from fungal tissue lysates. (omegabiotek.com)
  • We studied the fungal endophyte community of the leaves of Myracrodruon urundeuva in Brazil along with their potential to produce L-asparaginase. (scielo.br)
  • A fundamental problem in fungal systematics is the existence of teleomorphic and anamorphic stages in their life cycles. (wikipedia.org)
  • In conclusion, textile seats are much more contaminated by pet allergens and fungal DNA than leather seats. (diva-portal.org)
  • Fungal composition is significantly auto-correlated at the national and hemispheric spatial scales. (pnas.org)
  • Remarkably, building function has no significant effect on indoor fungal composition, despite stark contrasts between architecture and materials of some buildings in close proximity. (pnas.org)
  • Our results suggest that factors driving fungal composition are primarily global rather than mediated by building design or function. (pnas.org)
  • Recent efforts to describe the processes shaping indoor and urban fungal composition show temporal effects ( 13 - 15 ) and modest correlations with human activity ( 16 ). (pnas.org)
  • Finally, as a result of the combination of presumably high dispersal rates between indoor habitats and the highly selective indoor environment, we expected little influence of the outdoor environment on fungal composition. (pnas.org)
  • Soil type influenced the composition of the PU fungal communities. (asm.org)
  • In this study, physicochemical properties, contents of main pollutants, and fungal diversity of the aged oil sludge-contaminated soil were analyzed. (hindawi.com)
  • Until now, as the most deleterious components in AOS [ 9 ], the contents of total petroleum hydrocarbons (TPHs) and HMs as well as fungal diversity of the long-time oil-polluted soil are still unknown and rarely referred and reported globally. (hindawi.com)
  • Soil fungal communities involved in the biodegradation of polyester polyurethane (PU) were investigated. (asm.org)
  • This is the first study investigating the fungal communities that develop on the surface of plastics such as PU during burial in situ in soil. (asm.org)
  • This situation is perpetuated by a paucity of cultivable fungal strains available for phylogenetic research linked to these data sets. (duhnnae.com)
  • For the experiments with ryp T-DNA mutants, there were 4 to 6 replicates for each strain and condition, and for the experiments with ryp knockdown strains, there were 3 to 12 replicates for each strain and condition. (omictools.com)
  • Recently, several polymerase chain reaction (PCR)-based assays have been developed as rapid diagnostic tools for detecting fungal keratitis. (arvojournals.org)