The 3'-->5' exonucleases of DNA polymerases delta and epsilon and the 5'-->3' exonuclease Exo1 have major roles in postreplication mutation avoidance in Saccharomyces cerevisiae. (1/8779)
Replication fidelity is controlled by DNA polymerase proofreading and postreplication mismatch repair. We have genetically characterized the roles of the 5'-->3' Exo1 and the 3'-->5' DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using various genetic backgrounds and highly sensitive mutation detection systems that are based on long and short homonucleotide runs. Genetic interactions were examined among DNA polymerase epsilon (pol2-4) and delta (pol3-01) mutants defective in 3'-->5' proofreading exonuclease, mutants defective in the 5'-->3' exonuclease Exo1, and mismatch repair mutants (msh2, msh3, or msh6). These three exonucleases play an important role in mutation avoidance. Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes. (+info)Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. (2/8779)
Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex. (+info)Histone octamer transfer by a chromatin-remodeling complex. (3/8779)
RSC, an abundant, essential chromatin-remodeling complex related to SWI/SNF complex, catalyzes the transfer of a histone octamer from a nucleosome core particle to naked DNA. The newly formed octamer-DNA complex is identical with a nucleosome in all respects. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. The mechanism may entail formation of a duplex displacement loop on the nucleosome, facilitating the entry of exogeneous DNA and the release of the endogenous molecule. (+info)Regulation of the start of DNA replication in Schizosaccharomyces pombe. (4/8779)
Cells of Schizosaccharomyces pombe were grown in minimal medium with different nitrogen sources under steady-state conditions, with doubling times ranging from 2.5 to 14 hours. Flow cytometry and fluorescence microscopy confirmed earlier findings that at rapid growth rates, the G1 phase was short and cell separation occurred at the end of S phase. For some nitrogen sources, the growth rate was greatly decreased, the G1 phase occupied 30-50% of the cell cycle, and cell separation occurred in early G1. In contrast, other nitrogen sources supported low growth rates without any significant increase in G1 duration. The method described allows manipulation of the length of G1 and the relative cell cycle position of S phase in wild-type cells. Cell mass was measured by flow cytometry as scattered light and as protein-associated fluorescence. The extensions of G1 were not related to cell mass at entry into S phase. Our data do not support the hypothesis that the cells must reach a certain fixed, critical mass before entry into S. We suggest that cell mass at the G1/S transition point is variable and determined by a set of molecular parameters. In the present experiments, these parameters were influenced by the different nitrogen sources in a way that was independent of the actual growth rate. (+info)The prokaryotic beta-recombinase catalyzes site-specific recombination in mammalian cells. (5/8779)
The development of new strategies for the in vivo modification of eukaryotic genomes has become an important objective of current research. Site-specific recombination has proven useful, as it allows controlled manipulation of murine, plant, and yeast genomes. Here we provide the first evidence that the prokaryotic site-specific recombinase (beta-recombinase), which catalyzes only intramolecular recombination, is active in eukaryotic environments. beta-Recombinase, encoded by the beta gene of the Gram-positive broad host range plasmid pSM19035, has been functionally expressed in eukaryotic cell lines, demonstrating high avidity for the nuclear compartment and forming a clear speckled pattern when assayed by indirect immunofluorescence. In simian COS-1 cells, transient beta-recombinase expression promoted deletion of a DNA fragment lying between two directly oriented specific recognition/crossing over sequences (six sites) located as an extrachromosomal DNA substrate. The same result was obtained in a recombination-dependent lacZ activation system tested in a cell line that stably expresses the beta-recombinase protein. In stable NIH/3T3 clones bearing different number of copies of the target sequences integrated at distinct chromosomal locations, transient beta-recombinase expression also promoted deletion of the intervening DNA, independently of the insertion position of the target sequences. The utility of this new recombination tool for the manipulation of eukaryotic genomes, used either alone or in combination with the other recombination systems currently in use, is discussed. (+info)The yeast dynamin-like protein, Mgm1p, functions on the mitochondrial outer membrane to mediate mitochondrial inheritance. (6/8779)
The mdm17 mutation causes temperature-dependent defects in mitochondrial inheritance, mitochondrial morphology, and the maintenance of mitochondrial DNA in the yeast Saccharomyces cerevisiae. Defects in mitochondrial transmission to daughter buds and changes in mitochondrial morphology were apparent within 30 min after shifting cells to 37 degrees C, while loss of the mitochondrial genome occurred after 4-24 h at the elevated temperature. The mdm17 lesion mapped to MGM1, a gene encoding a dynamin-like GTPase previously implicated in mitochondrial genome maintenance, and the cloned MGM1 gene complements all of the mdm17 mutant phenotypes. Cells with an mgm1-null mutation displayed aberrant mitochondrial inheritance and morphology. A version of mgm1 mutated in a conserved residue in the putative GTP-binding site was unable to complement any of the mutant defects. It also caused aberrant mitochondrial distribution and morphology when expressed at high levels in cells that also contained a wild-type copy of the gene. Mgm1p was localized to the mitochondrial outer membrane and fractionated as a component of a high molecular weight complex. These results indicate that Mgm1p is a mitochondrial inheritance and morphology component that functions on the mitochondrial surface. (+info)All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature. (7/8779)
All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned. These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature. The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs. Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex. (+info)The nuclear ribosomal DNA intergenic spacer as a target sequence to study intraspecific diversity of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum directly on pinus root systems. (8/8779)
Polymorphism of the nuclear ribosomal DNA intergenic spacer (IGS) of the ectomycorrhizal basidiomycete Hebeloma cylindrosporum was studied to evaluate whether this sequence could be used in field studies to estimate the diversity of strains forming mycorrhizas on individual Pinus pinaster root systems. This sequence was amplified by PCR from 125 haploid homokaryotic strains collected in 14 P. pinaster stands along the Atlantic coast of France by using conserved oligonucleotide primers. Restriction enzyme digestion of the amplified 3.4-kbp-long IGS allowed us to characterize 24 alleles whose frequencies differed. Nine of these alleles were found only once, whereas about 60% of the strains contained four of the alleles. Local populations could be almost as diverse as the entire population along a 150-km stretch of coastline that was examined; for example, 13 alleles were found in a single forest stand. The IGS from one strain was partially sequenced, and the sequence data were used to design oligonucleotides which allowed separate PCR amplification of three different segments of the IGS. Most polymorphisms observed among the full-length IGS regions resulted from polymorphisms in an internal ca. 1,500-bp-long sequence characterized by length variations that may have resulted from variable numbers of a T2AG3 motif. This internal polymorphic sequence could not be amplified from the genomes of nine other Hebeloma species. Analysis of this internal sequence amplified from the haploid progenies of 10 fruiting bodies collected in a 70-m2 area resulted in identification of six allelic forms and seven distinct diplotypes out of the 21 possible different combinations. Moreover, optimization of the PCR conditions resulted in amplification of this sequence from more than 80% of the DNA samples extracted from individual H. cylindrosporum infected P. pinaster mycorrhizal root tips, thus demonstrating the usefulness of this sequence for studying the below-ground diversity of mycorrhizas formed by genets belonging to the same fungal species. (+info)
Genetic and genomic analysis of the AT-rich centromere DNA element II by Richard E. Baker and Kelly Rogers
Fungal Profiling and Ecology of the Healthy Human Gastrointestinal Tract | National Agricultural Library
The Awesome Power of Yeast Evolutionary Genetics: New Genome Sequences and Strain Resources for the Saccharomyces sensu stricto...
In Silico </i>Analysis of Structural Properties of Fungal DNA Sequences and Promoter...
Fungal and Yeast Genomic DNA Page 1
Fungal and Yeast Genomic DNA Page 5
CEN
| allnurses
Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins...
LAHOFER Rulandské šedé, neskorý zber 2013
Genetic analysis of Saccharomyces cerevisiae chromosome I: on the role of mutagen specificity in delimiting the set of genes...
YEP367.SEQ
YEP353.SEQ
cen.html
psd: Phylogenetic Species Diversity Metrics in picante: R tools for integrating phylogenies and ecology
Molecular typing demonstrates homogeneity of Saccharomyces uvarum strains and reveals the existence of hybrids between S....
JoVE Author Search: Litvintseva AP
Plant Life: Basidiomycetes
Search | Biocodex Microbiote Institute
Association of electrophoretic karyotype of Candida stellatoidea with virulence for mice. | Infection and Immunity
What is a phylogenetic species concept? | Reference.com
Yeast Plasmid Miniprep - Yeast Plasmid Isolation Kit
Yeast Plasmid Miniprep - Yeast Plasmid Isolation Kit
Mycotaxon: Volume 106 Article Abstract
Artemisinin and cancer, yeasts and parasites | CANCERactive
Cortinarius alboviolaceus, silkkiseitikki @ Natural Fungi in Finland
Find everything on fermentis yeast strains | Echemi
IIT Roorkee researchers discover treatment against drug-resistant fungal species
What Is DNA?- Meaning, DNA Types, Structure and Functions
如何逆轉第二型糖尿病 - TY 健康新知部落格
用音樂與歡笑來降低血壓吧。 - TY 健康新知部落格
Association of Fission Yeast Orp1 and Mcm6 Proteins with Chromosomal Replication Origins | Molecular and Cellular Biology
Environmental distribution and genetic diversity of vegetative compatibility groups determine biocontrol strategies to mitigate...
Hybrid genotypes in the pathogenic yeast Cryptococcus neoformans | Microbiology Society
New Saccharomyces Sequences 06/29/95
Karyotypic Variation within Clonal Lineages of the Rice Blast Fungus, Magnaporthe grisea | Applied and Environmental...
ASMscience | Color Plates
Detection of Pneumocystis carinii DNA in air samples : Likely environmental risk to susceptible persons. | Base documentaire |...
PDF] Major clades of Agaricales: a multilocus phylogenetic overview | Semantic Scholar
中国科学院昆明植物研究所机构知识库(KIB OpenIR): Major clades of Agaricales: a multilocus phylogenetic overview
ASMscience | Yeasts
C-4 methylsterol oxidase (P53045) - Yeast Metabolome Database
Journal: Southern forests / Subject: host range and Fusarium circinatum - PubAg Search Results
Trichoderma taxonomy 2020
Custom Chromo: First Yeast Chromosome Built From Scratch | KUNM
Download Agaricales Capnoides Euagarics Floor Fungus Hypholoma Nobo...
Cortinarius cumatilis, posliiniseitikki @ Natural Fungi in Finland
Category:Ascobolus stercorarius - Wikimedia Commons
Brettanomyces yeast
Winter Agaricales Fungi
genital fungus - Symptoms, Treatments and Resources for genital fungus
No Szmoking:Hol a pénz,hol a.., kozma tibor, no szmoking, környei attia - Videa
New YEAST POWDER 100 gr. - Carpeco
fungus
- Kids | Britannica Kids | Homework Help
Fungus!
真菌 - 维基百科,自由的百科全书
Geotrichum candidum
Alper, I; Michel, F; Labrie, S (2011). "Ribosomal DNA Polymorphisms in the Yeast Geotrichum candidum". Fungal Biology. 115 (12 ... Fungal plant pathogens and diseases, Fungal citrus diseases, Animal fungal diseases, Organisms breaking down plastic, Fungi ... Palermo, A.M., Gentile, A. & Pellegrino, G (2021-10-19). "Documentary heritage: fungal deterioration in Compact Discs". Herit ... Fungal growth can be supported by D-glucose, D-mannose, D-xylose, L-sorbose, D-fructose, D-galactose, sucrose, D-mannitol, D- ...
Point mutation
DNA replication occurs when one double-stranded DNA molecule creates two single strands of DNA, each of which is a template for ... Because RIP is so efficient at detecting and mutating repeats, fungal biologists often use it as a tool for mutagenesis. A ... These reactants can lead to both single-stranded DNA breaks and double-stranded DNA breaks. Third, bonds in DNA eventually ... It is a DNA methyltransferease-like protein, that when mutated or knocked out results in loss of RIP. Deletion of the rid ...
Blastocladiomycota
Molecular phylogeny of the Blastocladiomycota (Fungi) based on nuclear ribosomal DNA. Fungal Biology 115:381-392. Kendrick, ... Fungal Diversity. 90 (1): 135-159. doi:10.1007/s13225-018-0401-0. Hibbett DS et al. 2007. A higher-level phylogenetic ...
Allomyces
... based on nuclear ribosomal DNA". Fungal Biology. 115: 381-392. doi:10.1016/j.funbio.2011.02.004. 6. Allomyces. Phillip W. ...
Reference genome
As a result of how the DNA samples were processed, about 80 percent of the reference genome came from eight people and one male ... "1000 Fungal Genomes Project". mycocosm.jgi.doe.gov. Retrieved 2022-08-18. Genome Reference Consortium (Genome projects, ... As they are assembled from the sequencing of DNA from a number of individual donors, reference genomes do not accurately ... Instead a reference provides a haploid mosaic of different DNA sequences from each donor. For example, the most recent human ...
Physodermataceae
... based on nuclear ribosomal DNA". Fungal Biology. 115 (4): 381-392. doi:10.1016/j.funbio.2011.02.004. ISSN 1878-6146. PMID ...
Physoderma
Molecular phylogeny of the Blastocladiomycota (Fungi) based on nuclear ribosomal DNA. Fungal Biology 115: 381-392. James, T.Y ...
Nigrospora sphaerica
Fungal spores found in the body of the nail resembled the characteristic morphology of N. sphaerica. DNA sequence analysis ... Fungal spores trapped on the agar strips were developed and counted. They were then cultured into isolates allowing for ... A woman in south India was diagnosed with a fungal corneal ulcer after being hit in the eye from a cow's tail. Analysis of ... It was hypothesized that this special case of fungal corneal ulcer was caused by transfer of spores to the patients eye from ...
Lactarius
Ochricompactae" (PDF). Fungal Diversity. 28: 15-40. Eberhardt U, Verbeken A (2004). "Sequestrate Lactarius species from ... Russulales; Basidiomycota) in Alaska, based on soil and sporocarp DNA" (PDF). Molecular Ecology. 18 (10): 2213-2227. doi: ... Rinaldi AC, Comandini O, Kuyper TW (2008). "Ectomycorrhizal fungal diversity: separating the wheat from the chaff" (PDF). ... Twieg BD, Durall DM, Simard SW (2007). "Ectomycorrhizal fungal succession in mixed temperate forests". New Phytologist. 176 (2 ...
Phylloporus
... morphological and multilocus DNA sequence analyses". Fungal Diversity. 58 (1): 73-101. doi:10.1007/s13225-012-0184-7. Neves MA ... Fungal Diversity. 55 (1): 109-123. doi:10.1007/s13225-012-0154-0. "Phylloporus on Boletineae: Surveys and Revisions". " ...
Phylloporus leucomycelinus
... morphological and multilocus DNA sequence analyses". Fungal Diversity. 58: 73-101. doi:10.1007/s13225-012-0184-7. S2CID ... Fungal Diversity. 55 (1): 109-23. doi:10.1007/s13225-012-0154-0. S2CID 17272328. Zheng NK, Tang LP, Li YC, Tolgor B, Zhu XT, ...
Copper fist
... is an N-terminal domain involved in copper-dependent DNA binding. It is named for its resemblance to a fist. It can ... be found in some fungal transcription factors. These proteins activate the transcription of the metallothionein gene in ... which allows DNA binding. Copper fist definition Turner RB, Smith DL, Zawrotny ME, Summers MF, Posewitz MC, Winge DR (July 1998 ...
Skeletocutis
This species is part of the community of fungal successors of decaying wood. A Finnish study found that it fruited most ... "Phylogenetic relationships in European Ceriporiopsis species inferred from nuclear and mitochondrial ribosomal DNA sequences". ... Yu, Chang-Jun; Zuo, Li; Dai, Yu-Cheng (2005). "Three polypores from Xizang new to China" (PDF). Fungal Science. 20 (3-4): 61-68 ... "Skeletocutis nothofagi Rajchenb". The Global Fungal Red List Initiative. IUCN. Retrieved 30 September 2016. Fries, Elias Magnus ...
Agaricus
"Phylogeny of the genus Agaricus inferred from restriction analysis of enzymatically amplified ribosomal DNA". Fungal Genet Biol ... Based on DNA analysis of ITS1, ITS2, and 5.8S sequences, the studied species of this section could be divided into six distinct ... The fungal genus Agaricus as late as 2008 was believed to contain about 200 species worldwide but since then, molecular ...
Galzinia
2016). "Fungal diversity notes 253-366". Fungal Divers. 78. doi:10.1007/s13225-016-0366-9.{{cite journal}}: CS1 maint: uses ... Molecular research, based on cladistic analysis of DNA sequences, suggests the genus may be polyphyletic. The type species has ...
Fusarium subglutinans
DNA sequencing data reveals the presence of two major groups representing cryptic species in F. subglutinans. These were ... Fusarium subglutinans is a fungal plant pathogen. Fusarium subglutinans is the anamorph of Gibberella fujikuroi. Fusarium ... Fungal plant pathogens and diseases, Maize diseases, Mango tree diseases, Fungi described in 1925, All stub articles, ...
Rebecca Yahr
2022) What do the first 597 global fungal red list assessments tell us about the threat status of fungi? Diversity 14 (9), 736 ... 2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of ... V., Spouge, J.L., Levesque, C.A., … and Fungal Barcoding Consortium. ( ...
Flucytosine
... also undergoes conversion into 5-fluorodeoxyuridinemonophosphate which inhibits fungal DNA synthesis. Flucytosine ... Serious fungal infections may occur in those who are immunocompromised. These people benefit from combination therapy including ... Flucytosine must not be used as a sole agent in life-threatening fungal infections due to relatively weak antifungal effects ...
John W. Taylor (professor)
As DNA sequencing costs dropped, they used population genetics to recognize fungal species and describe them, based solely on ... Taylor, John W. (December 2011). "One Fungus = One Name: DNA and fungal nomenclature twenty years after PCR". IMA Fungus. 2 (2 ... and Fungal Kingdom: Threats and Opportunities (2019-2024). In 1990, Taylor's lab group published protocols for fungal PCR that ... DNA sequence data were then used to detect recombination in fungi for which sex had never been observed, despite years of ...
Polyploidy
Although the replication and transcription of DNA is highly standardized in eukaryotes, the same cannot be said for their ... Besides plants and animals, the evolutionary history of various fungal species is dotted by past and recent whole-genome ... Lidzbarsky, G. A.; Shkolnik, T.; Nevo, E. (2009). Idnurm, A. (ed.). "Adaptive Response to DNA-Damaging Agents in Natural ... Indeed, homoploid speciation (hybrid speciation without a change in chromosome number) has been evidenced for some fungal ...
Dolichousnea
Lücking, Robert; Nadel, Miko; Araujo, Elena; Gerlach, Alice (2020). "Two decades of DNA barcoding in the genus Usnea ( ... Fungal Diversity. 84 (1): 101-117. doi:10.1007/s13225-017-0379-z. Ohmura 2001, p. 8. Ohmura 2001, p. 9. Ohmura 2001. Ohmura ... Parmeliaceae): how useful and reliable is the ITS?". Plant and Fungal Systematics. 65 (2): 303-357. doi:10.35535/pfsyst-2020- ...
Nematoctonus
2011). "One Fungus = One Name: DNA and fungal nomenclature twenty years after PCR". IMA Fungus. 2 (2): 113-20. doi:10.5598/ ...
Serpin
A single fungal serpin has been characterized to date: celpin from Piromyces spp. strain E2. Piromyces is a genus of anaerobic ... December 2007). "DNA accelerates the inhibition of human cathepsin V by serpins". The Journal of Biological Chemistry. 282 (51 ... Ahn JW, Atwell BJ, Roberts TH (May 2009). "Serpin genes AtSRP2 and AtSRP3 are required for normal growth sensitivity to a DNA ... Two other Arabidopsis serpins, AtSRP2 (At2g14540) and AtSRP3 (At1g64030) appear to be involved in responses to DNA damage. ...
Phytophthora alni
Fungal Genet. Biol. 43 (7): 511-29. doi:10.1016/j.fgb.2006.02.006. PMID 16626980. S2CID 43275127. Chandelier A, Abras S, ... "Genetic characterization of the natural hybrid species Phytophthora alni as inferred from nuclear and mitochondrial DNA ...
Cunninghamella elegans
"A method for the large scale isolation of high transformation efficiency fungal genomic DNA". FEMS Microbiology Letters. 145 (2 ... Cerniglia, C. E.; Mahaffey, W.; Gibson, D. T. (1980). "Fungal oxidation of benzo\a]pyrene: Formation of (−)-trans-7,8-dihydroxy ... Pothuluri, J. V.; Freeman, J. P.; Evans, F. E.; Cerniglia, C. E. (1992). "Fungal metabolism of acenaphthene by Cunninghamella ... Methods for efficient C. elegans genomic DNA isolation and transformation have been developed. The cytochrome P450 of C. ...
White-nose syndrome
Lindner DL, Gargas A, Lorch JM, Banik MT, Glaeser J, Kunz TH, Blehert DS (2011-03-18). "DNA-based detection of the fungal ... Researchers suspect through DNA analysis that the source of infection in this individual originated in the Eastern U.S. This ... "Fungal disease sapping bats all over Georgia". Savannah Now. 19 May 2016. Retrieved 21 May 2016. "Bats affected by WNS". White- ... White-nose syndrome (WNS) is a fungal disease in North American bats which has resulted in the dramatic decrease of the bat ...
Turbinellus stereoides
... and mit-atp6-DNA combined sequences". Fungal Biology. 114 (2-3): 224-34. doi:10.1016/j.funbio.2010.01.002. PMID 20943133. ...
Turbinellus floccosus
... and mit-atp6-DNA Combined Sequences". Fungal Biology. 114 (2-3): 224-34. doi:10.1016/j.funbio.2010.01.002. PMID 20943133. ... Khaund P, Joshi SR (2014). "DNA Barcoding of Wild Edible Mushrooms Consumed by the Ethnic Tribes of India". Gene. 550 (1): 123- ... T. floccosus also contains the dicatecholspermidine derivative pistillarin, a compound that inhibits DNA damage by hydroxyl ... Inhibits DNA Single Strand Breakage by the Fenton Reaction". Journal of the Korean Society for Applied Biological Chemistry. 54 ...
Turbinellus kauffmanii
... and mit-atp6-DNA combined sequences". Fungal Biology. 114 (2-3): 224-34. doi:10.1016/j.funbio.2010.01.002. PMID 20943133. ...
Turbinellus fujisanensis
... and mit-atp6-DNA combined sequences". Fungal Biology. 114 (2-3): 224-34. doi:10.1016/j.funbio.2010.01.002. PMID 20943133. ...
Species
... to each other need to be checked by DNA-DNA hybridisation to decide if they belong to the same species.[20] This concept was ... "Fungal Genetics and Biology. 31 (1): 21-32. doi:10.1006/fgbi.2000.1228. PMID 11118132. S2CID 2551424.. ... DNA barcoding and phylogenetics are commonly used in these cases.[73][74][75] The term quasispecies is sometimes used for ... a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology". ...
Pleural effusion
A polymerase chain reaction for tuberculous DNA may be done, or adenosine deaminase or interferon gamma levels may also be ... Other tests as suggested by the clinical situation - lipids, fungal culture, viral culture, tuberculosis cultures, lupus cell ...
Bryozoa
A study of the mitochondrial DNA sequence suggests that the Bryozoa may be related to the Chaetognatha.[80] ... "Exceptional soft-tissue preservation in boring ctenostome bryozoans and associated "fungal" borings from the Early Devonian of ... "The higher phylogeny of phylactolaemate bryozoans inferred from 18S ribosomal DNA sequences" (PDF). In Moyano, H. I.; Cancino ...
Chitinase
Other plant chitinases may be required for creating fungal symbioses.[9] Although mammals do not produce chitin, they have two ... Expression is mediated by the NPR1 gene and the salicylic acid pathway, both involved in resistance to fungal and insect attack ... Langner T, Göhre V (May 2016). "Fungal chitinases: function, regulation, and potential roles in plant/pathogen interactions". ... as an anti-fungal remedy, an anti-tumor drug and as a general ingredient to be used in protein engineering.[42] ...
Plant
... and double-strand breaks in DNA can be repaired.[79] Double-strand repair in plants often produce DNA junctions with structural ... The fungal endophyte, Neotyphodium coenophialum, in tall fescue (Festuca arundinacea) does tremendous economic damage to the ... Plants are capable of a DNA damage response that is a critical mechanism for maintaining genome stability.[76] The DNA damage ... 89 The DNA checkpoint kinase ATM has a key role in integrating progression through germination with repair responses to the DNA ...
Bioindicator
Techniques used for MPOG include DNA analysis, simple bug counts after culturing a soil sample in a hydrocarbon-based medium or ... Plant and fungal indicatorsEdit. The lichen Lobaria pulmonaria is sensitive to air pollution. ...
Ffwng - Wicipedia
Jennings DH, Lysek G (1996). Fungal Biology: Understanding the Fungal Lifestyle. Guildford, UK: Bios Scientific Publishers Ltd ... Yn yr 20fed a'r 21g, mae datblygiadau mewn biocemeg, geneteg, bioleg foleciwlaidd, biotechnoleg, dilyniannu DNA a dadansoddi ... "Fungal secondary metabolism - from biochemistry to genomics". Nature Reviews. Microbiology 3 (12): 937-47. December 2005. doi: ... morphology in understanding fungal relationships and biodiversity". Revista Iberoamericana de Micología 23 (3): 127-33. ...
白色念珠菌 - 维基百科,自由的百科全
链格孢病 · 真菌毛囊炎(英语:Fungal folliculitis) · 镰刀菌属/病 · 婴儿臀部肉芽肿(英语:Granuloma gluteale infantum) · 透明丝孢霉病 · 真菌性耳炎(英语:Otomycosis) · 暗色丝 ... 有絲分裂時Spo11蛋白可以切割DNA,促使同源染色體間發生同源重組[76][79]。 ... Fungal Genetics and Biology. 2009, 46 (2): 210-9. PMC 2698078 . PMID 19032986. doi:10.1016/
List of parasites of humans
DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). ... Fungal infection and mesomycetozoea. Superficial and. cutaneous. (dermatomycosis):. Tinea = skin;. Piedra (exothrix/. endothrix ... DNA virus. HBV Hepatocellular carcinoma. HPV Cervical cancer. Anal cancer. Penile cancer. Vulvar cancer. Vaginal cancer. ... DNA virus. Human polyomavirus 2 Progressive multifocal leukoencephalopathy. RNA virus. MeV Subacute sclerosing panencephalitis ...
Grafting
... can transfer chloroplasts (plant organelles that can conduct photosynthesis), mitochondrial DNA and the entire cell ... an insect that infests the roots of vines and causes fungal infections. Initially, farmers unsuccessfully attempted to contain ...
Aspirin
Since platelets have no DNA, they are unable to synthesize new PTGS once aspirin has irreversibly inhibited the enzyme, an ... A role of aspirin on bacterial and fungal biofilm is also being supported by growing evidence.[217] ...
Earliest known life forms
"DNA Damage and Survival Time Course of Deinococcal Cell Pellets During 3 Years of Exposure to Outer Space". Frontiers in ...
Plant reproductive morphology
Bernstein, C. & Bernstein, H. (1991). Aging, Sex, and DNA Repair. San Diego: Academic Press. ISBN 978-0-12-092860-6. .. ... Fungal reproduction. *Sexual reproduction in animals *Sexual intercourse. *Copulation. *Human reproduction. *Lordosis behavior ...
Candida blankii
Blood sample DNA sequencing of the 26S ribosomal subunit can definitively identify C. blankii. In nature, Candida blankii forms ... Fungal blood-stream infections (fungaemia) have been newly associated with C blankii. Polyene antifungals have been identified ...
Kew Gardens
... a plant DNA bank and a seed bank. The Kew Fungarium houses approximately 1.25 million specimens of dried fungi. The Library, ... has over 8.5 million preserved plant and fungal specimens. The library contains more than 750,000 volumes, and the ... a quarterly peer-reviewed scientific journal on plant and fungal taxonomy published by Springer Science+Business Media on ...
Genome size
Animal Genome Size Database Plant DNA C-values Database Fungal Genome Size Database Fungal Database Archived 2008-03-10 at the ... genome sizes Human genome Junk DNA List of sequenced eukaryotic genomes Non-coding DNA Plant DNA C-values Database Selfish DNA ... Some single-celled organisms have much more DNA than humans, for reasons that remain unclear (see non-coding DNA and C-value ... Genome size is the total amount of DNA contained within one copy of a single complete genome. It is typically measured in terms ...
Sparassis
Phylogenetic relationships of Sparassis inferred from nuclear and mitochondrial ribosomal DNA and a protein-coding gene (rpb2 ... Articles with 'species' microformats, Edible fungi, Taxa described in 1819, Fungi in cultivation, Polyporales, Fungal tree ...
Interferon
Moiseeva O, Mallette FA, Mukhopadhyay UK, Moores A, Ferbeyre G (April 2006). "DNA Damage Signaling and p53-dependent Senescence ... recent information demonstrates the importance of Type III IFNs in some types of virus or fungal infections. In general, type I ... Interferon was scarce and expensive until 1980, when the interferon gene was inserted into bacteria using recombinant DNA ... which leads to expression of proteins that will prevent the virus from producing and replicating its RNA and DNA. Overall, IFN- ...
Mitochondrial DNA
Type 2, type 3 and type 5 mentioned in the plant and fungal genomes also exist in some protists, as do two unique genome types ... Mitochondrial DNA is replicated by the DNA polymerase gamma complex which is composed of a 140 kDa catalytic DNA polymerase ... Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus and ... one precious model for organelle DNA inheritance and evolution". DNA and Cell Biology. 28 (2): 79-89. doi:10.1089/dna.2008.0807 ...
Topical antifungal drugs
Finally, it disrupts the synthesis of DNA, RNA and proteins in fungal cells and leads to cell death. Ciclopirox may also exert ... preventing further reproduction of fungal cells. Lastly, antifungal agents contribute to fungal cell death. Fungal infections ... Fungal skin infections Nystatin in the forms of creams, ointments or powders can also be used to treat fungal infections of the ... By blocking the sterol synthesis in fungal cells and cell extracts, the integrity of fungal cell membranes will be interrupted ...
Aflatoxin B1
"Definition of Aspergillosis , Aspergillosis , Types of Fungal Diseases , Fungal Diseases , CDC". www.cdc.gov. Retrieved 2017-05 ... These adducts and alterations represent lesions which, upon DNA replication cause the insertion of a mis-matched base in the ... This active form then intercalates between DNA base residues and forms adducts with guanine residues, most commonly aflatoxin ... "Aspergillus flavus :: Center for Integrated Fungal Research". www.cifr.ncsu.edu. Retrieved 2017-05-08. " ...
Batrachochytrium dendrobatidis
DNA analysis of the SSU-rDNA has corroborated the view, with the closest match to Chytridium confervae. A second species of ... The fungal pathogens that cause the disease chytridiomycosis ravage the skin of frogs, toads, and other amphibians, throwing ...
Cell cycle
If the DNA is damaged, p53 will either repair the DNA or trigger the apoptosis of the cell. If p53 is dysfunctional or mutated ... In cells with nuclei (eukaryotes, i.e., animal, plant, fungal, and protist cells), the cell cycle is divided into two main ... DNA replication occurs during the C period. The D period refers to the stage between the end of DNA replication and the ... It is estimated that in normal human cells about 1% of single-strand DNA damages are converted to about 50 endogenous DNA ...
Truffle
The majority of fungal biomass is found in the humus and litter layers of soil. Most truffle fungi produce both asexual spores ... of mitochondrial DNA. The Magnatum and Macrosporum clades were distinguished as distinct from the Aestivum clade. The Gibbosum ... Many ectomycorrhizal fungi form fungal mats in the upper layers of soils surrounding host plants. These mats have significantly ... of nuclear DNA and revealed five major clades (Aestivum, Excavatum, Rufum, Melanosporum and Puberulum); this was later improved ...
Alternaria japonica
In seedlings, fungal lesions on the stem are a cause of damping-off. Infected seeds appear black or grey. The fungus can first ... Sequencing of the ribosomal DNA is commonly used for positive identification because the symptoms and microscopic appearance ... Alternaria japonica is a fungal plant pathogen. It is a cause of black spot disease in cruciferous plants. It is not a major ... Fungal plant pathogens and diseases, Fungi described in 1941). ...
Ramaria cokeri
... and mit-atp6-DNA combined sequences" (PDF). Fungal Biology. 114 (2-3): 224-34. doi:10.1016/j.funbio.2010.01.002. PMID 20943133 ...
Microsporum canis
Shafiee, Shabnam; Khosravi, Ali Reza; Tamai, Iradj Ashrafi (2014). "Comparative study of Microsporum canis isolated by DNA ... of the fungus from brushed pet hair can aid in detection of either an actively growing fungus or a passive carriage of fungal ...
Biometal (biology)
Processes including oxygen transport and DNA replication are carried out using enzymes such as DNA polymerase, which in humans ... Zinc was identified to be essential in fungal growth of yeast as shown by Jules Raulin in 1869 yet no proof for the need of ... Hydroxyl radicals and other reactive oxygen species when generated near DNA can cause point mutations, cross-linkage and breaks ...
Herbal medicine
Twenty-four products were tested by DNA barcoding as part of the investigation, with all but five containing DNA that did not ... The scope of herbal medicine commonly includes fungal and bee products, as well as minerals, shells and certain animal parts. ... 2012). "Deep sequencing of plant and animal DNA contained within traditional Chinese medicines reveals legality issues and ... Newmaster SG, Grguric M, Shanmughanandhan D, Ramalingam S, Ragupathy S (October 2013). "DNA barcoding detects contamination and ...
Crinipellis zonata
The conclusions of this analysis, based on the DNA sequences of ribosomal DNA coding for internal transcribed spacers, are ... Cerniglia CE (1997). "Fungal metabolism of polycyclic aromatic hydrocarbons: Past, present and future applications in ...
Target Genes, Primer Sets, and Thermocycler Settings for Fungal DNA Amplification | Fungal Diseases | CDC
This tool assumes that fungal DNA already exists; it does not describe the procedure for purification of fungal DNA. ... Target Genes, Primer Sets, and Thermocycler Settings for Fungal DNA Amplification. *Anamorph and Teleomorph Names for Candida ... Target Genes, Primer Sets, and Thermocycler Settings for Fungal DNA Amplification. ... Trichophyton species DNA is amplified very poorly by the ITS primer set used for most other molds. There is a special set of ...
Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing
DNA, Fungal Fungi Genes, RRNA Mycological Typing Techniques Polymerase Chain Reaction Sequence Analysis, DNA ... Assessment of fungal diversity in a water-damaged office building Cite CITE. Title : Assessment of fungal diversity in a water- ... Title : Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing Personal Author(s) : ... Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing. ...
DNA extraction and fungal communities sequencing - Project topics materials
DNA extraction and fungal communities sequencing. On the 20th of July 2011, foliar disks were excised from the dried leaves as ... DNA extraction and fungal communities sequencing. Statistical analyses. Results. Discussion. References. 3 QTLs for microbial ... Leaf DNA extraction. Amplification and sequencing of the fungal assemblages. Amplification and sequencing of the bacterial ... You Are Here Home Science laboratory technology project topics DNA extraction and fungal communities sequencing ...
Frontiers | Hatching of Globodera pallida Induced by Root Exudates Is Not Influenced by Soil Microbiota Composition
Characterization of Bacterial and Fungal Communities. Microbial DNA Sample Preparation. Soil DNA extractions were performed ... For the next steps of DNA extraction, samples were duplicated in order to obtain a higher DNA concentration. De-proteinization ... The fungal community composition was driven by the type of soil (F = 13.72, df = 1, p = 0.001), the soil (F = 13.83, df = 3, p ... In fungal communities, the number of ASVs was influenced by the type of soil (χ2 = 15.41, df = 1, p , 0.0001), the soil (χ2 = ...
Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from...
... l of DNA, respectively, for,i, Fusarium,/i,,,i, A. flavus,,/i, and,i, A. fumigatus.,/i, Alarming fact was that,i, Fusarium ,/i, ... Among fungal aetiologies,,i, Fusarium ,/i,(216, 52.5% of 411) and,i, Aspergillus ,/i,(68, 16.5% of 411) were ... While the study revealed a male preponderance with both the fungal keratitis , it further brought out that polyene compounds ( ... Fungal aetiology of keratitis/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in ...
Removal of Fungal Contaminants and their DNA from the Surface of Diaphorina citri (hemiptera: psyllidae) Prior to a Molecular...
Removal of Fungal Contaminants and their DNA from the Surface of Diaphorina citri (hemiptera: psyllidae) Prior to a Molecular ... Jason M. Meyer and Marjorie A. Hoy "Removal of Fungal Contaminants and their DNA from the Surface of Diaphorina citri ( ... PCR products were obtained for the fungal LSU using template DNA isolated from D. citri treated with all of the other ... A high-fidelity PCR protocol was used to amplify the fungal 28S rRNA gene (large ribosomal subunit: LSU) with template DNA ...
Immune Modulators as Adjuncts in Invasive Fungal Infections
Aspergillosis: IFN-γ DNA delivered to mouse lungs protects against aspergillosis. Cryptococcosis: IFN-γ potentiates the ... Associated with septic shock and breakthrough fungal infections. [45-47]. IL-15. NA. Increases antifungal activity of PMNL. -. ... Randomized Phase III trial: no effect on survival after invasive fungal disease. NA. Lack of benefit may be related to delayed ... Did not reduce fungal-related mortality in patients undergoing induction chemotherapy for AML. Prolonged survival of ...
MARPLE, a point-of-care, strain-level disease diagnostics and surveillance tool for complex fungal pathogens | BMC Biology |...
However, their application to complex fungal pathogens has remained limited due to the frequent inability to culture these ... We used targeted sequencing to overcome limitations associated with the size of fungal genomes and their often obligately ... point-of-care approach specifically tailored to identify individual strains of complex fungal plant pathogens. ... Excess RNAlater® was removed, and ~ 10-20 mg of tissue was used for each DNA extraction. DNA was extracted using a DNeasy 96 ...
PDF] Monitoring endangered freshwater biodiversity using environmental DNA. | Semantic Scholar
Our findings underpin the ubiquitous nature of DNA traces in the environment and establish environmental DNA as a tool for ... underpin the ubiquitous nature of DNA traces in the environment and establish environmental DNA as a tool for monitoring rare ... We further demonstrate that entire faunas of amphibians and fish can be detected by high-throughput sequencing of DNA extracted ... We successfully validate our findings in a controlled mesocosm experiment and show that DNA becomes undetectable within 2 weeks ...
JoF | Free Full-Text | Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its...
ZnO NPs were synthesized from fungal secondary metabolites of mono cultures of Trichoderma harzianum (PGT4), Trichoderma reesei ... The fungal secondary metabolite crude was extracted from the mono and co-culture of Trichoderma spp. And were analyzed by GC-MS ... Standard techniques were used for DNA isolation, agarose gel electrophoresis and PCR amplification. Three fungal cultures of ... From seven-day-old, culture the fungal discs were pierced using a sterile cork borer of 5 mm in diameter. The fungal discs were ...
A human cell-surface receptor for xenotropic and polytropic murine leukemia viruses: possible role in G protein-coupled signal...
NIOSHTIC-2 Search Results - Full View
Terms: DNA or Deoxyribonucleic acid OR DNA or Deoxyribonucleic acid 4 - 4 of 581 Bibliographic entries ... We conducted an environmental study to characterize fungal communities in classroom floor dust. We collected 500 samples from ... Genomic DNA from dust was extracted for internal transcribed spacer 1 Illumina MiSeq sequencing to identify operational ... We identified 62 yeast genera, representing 19.6% of DNA sequences. Cyberlindnera was the most abundant (6.1%), followed by ...
Identification of Candida dubliniensis in a diagnostic microbiology laboratory
Could Trees Benefit from Fungal Transplants? | Inside Science
The cost of DNA sequencing is declining. The Department of Energys Joint Genome Institute has a library of genomes for well ... Averill said the fungal microbiome might be just an indicator of other underlying factors that contribute to tree growth. But ... Also, scientists used to think they had to keep soil samples super cold to preserve the DNA, but theyve learned they can just ... "We were like, Heres where youre going to find high levels of fungal biodiversity, and of fungi that are not going to be ...
DNA stable-isotope probing delineates carbon flows from rice residues into soil microbial communities depending on...
We combined DNA stable-isotope (13C) probing and high-throughput sequencing to identify active fungal and bacterial groups ... Thus, 13C-labeled rice residues were employed and DNA stable-isotope probing (DNA-SIP) combined with high-throughput sequencing ... DNA stable-isotope probing delineates carbon flows from rice residues into soil microbial communities depending on ... The fungal species (Jugulospora rotula and Emericellopsis terricola in NPK and NPKM soils, respectively) were identified as ...
Frank Martin : USDA ARS
Genetic characterization of Phytophthora nicotianae by the analysis of polymorphic regions of the mitochondrial DNA. Fungal ... sporangia using mitochondrial DNA markers for disease management of cucurbit downy mildew - (Peer Reviewed Journal) ... Pathogenomics-based Development of Crop-specific Diagnostics Tools for Emerging and Expanding Fungal Diseases in the U.S. ... Quantification of Fusarium oxysporum disease potential from soil DNA - (Proceedings) Matson, M.E., Crouch, U., Kane, S.M., ...
Uncovering fungal community composition in natural habitat of Ophiocordyceps sinensis using high-throughput sequencing and...
The fungal communities in external mycelial cortices were significantly distinct from soil microhabitat. Meanwhile, seven types ... A total of 280,519 high-quality sequences, belonging to 5 fungal phyla, 15 classes, 41 orders, 79 families, 112 genera, and 352 ... Therefore, the present study aimed to unravel fungal communities of natural O. sinensis using combination of high-throughput ... Our result reveal a comprehensive understanding of fungal community structure of natural O. sinensis, provide new insight into ...
Medical Science Monitor | Circulating Methylated SEPT9 DNA Analyses to Predict Recurrence Risk and Adjuvant Chemotherapy...
We aimed to evaluate the ability of circulating cell-free methylated SETP9 DNA (mSEPT9) to identify recurrence and to determine ... Editorial: The World Health Organization (WHO) Fungal Priority Pathogens List in Response to Emerging Funga... Med Sci Monit ... Positive (methylated SEPT9 DNA) and negative controls (SEPT9 DNA) were run in parallel with samples each time as quality ... Circulating Methylated DNA Analyses to Predict Recurrence Risk and Adjuvant Chemotherapy Benefit in Stage II to III Colorectal ...
Notes from the Field: Fatal Fungal Soft-Tissue Infections After a Tornado --- Joplin, Missouri, 2011
... and DNA sequencing; specimens from all 13 patients yielded the Mucormycete Apophysomyces trapeziformis. Further laboratory and ... and CDC began assisting MODHSS with identification of fungal isolates. By June 10, eight patients with necrotizing fungal soft- ... Notes from the Field: Fatal Fungal Soft-Tissue Infections After a Tornado --- Joplin, Missouri, 2011. On May 22, 2011, at 5:34 ... CDC received 48 clinical specimens, including 32 fungal isolates and 16 tissue blocks collected from wounds for microscopic ...
Urban stress is associated with variation in microbial species composition-but not richness-in Manhattan | The ISME Journal
Here we analyzed bacterial, fungal and ant communities in small road medians (average area 0.0008 km2) and larger parks ( ... Fungal DNA was sequenced for 26 sites (13 parks and 13 medians; Supplementary Figure S1) using the internal transcribed spacer ... Microbial DNA was isolated using the Power Soil Extraction Kit (MoBio, Carlsbad, CA, USA), and three DNA extractions were ... Similarly, there were no significant differences between any of the 10 most common fungal classes (Figure 4b). Only one fungal ...
Search Results | APMA
Methods: Molecular-age medicine now includes DNA-based tools to universally assess any microbe or pathogen with a known DNA ... To date, the presumptive infectious etiologies include classically-reported fungal dermatophytes, non-dermatophyte molds, and ... However, molecular-age medicine now includes DNA-based tools to universally assess any microbe or pathogen with a known DNA ... Results: Using DNA sequencing-based diagnostics, the results in this article document the first identification and ...
Staff Profiles : Institute of Biological, Environmental and Rural Sciences , Aberystwyth University
Timothy BARONI | Distinguished Professor (Full) - Emeritus | PhD | State University of New York College at Cortland, NY | SUNY...
Considerations and consequences of allowing DNA sequence data as types of fungal taxa ... A synopsis of Daedalea sensu stricto is provided based on morphological and DNA sequence data. Analyses indicate that at least ... The distinctive basidiospore form and a three locus DNA analysis separate the species that share these characteristics from ... the single-lineage Macrotermitinae has additionally acquired a fungal symbiont that complements digestion of food outside the ...
Browsing University Museum of Bergen by Author "Adamčík, Slavomír"
Assessment of microbiological contamination in the work environments of museums, archives and libraries | SpringerLink
Halstensen, A. S. (2008). Species-specific fungal DNA in airborne dust as surrogate for occupational mycotoxin exposure. ... 2009). Fungal diversity in ancient documents. A case study on the Archive of the University of Coimbra. International ... The dominant fungal aerosol fractions had aerodynamic diameters that ranged between 1.1 and 2.1 µm in museum storerooms. We ... Percentage contribution of each size fraction (aerodynamic diameter I-VI) of fungal bioaerosol. a Warehouse 2 in Museum II; b ...
Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU...
Studies of fungal communities using the canonical ITS barcode require corroboration across additional loci. Phylogenetic ... Here we link metabarcoding from the ITS2 and LSU D1-D2 regions to characterise fungal communities associated with bark beetles ... Full taxonomic characterisation of fungal communities is necessary for establishing ecological associations and early detection ... The DNA extracts were then used for fungal metabarcoding of the ITS2 region with primers ITS86F (5′-GTGAATCATCGAATCTTTGAA-3′) ( ...
Subject term: dieback - PubAg Search Results
Chrysanthemoides monilifera; Coprinellus; DNA barcoding; Diaporthe; Penicillium; death; dieback; endophytes; fungal communities ... Fungal biology 2022 v.126 no.8 pp. 480-487. ISSN:. 1878-6146. Subject:. Bacillus subtilis; Eutypa lata; Phaeoacremonium ... 4. Antioxidants and iron chelators inhibit oxygen radical generation in fungal cultures of plant pathogenic fungi ... This study is the first report of a postharvest kiwifruit rot in South Korea caused by Diaporthe eres and related fungal ...
Crippled fungus acts as vaccine
for similar vaccines against more common fungal threats, say researchers.. An inhabitant of soil, B. dermatitidis frequently ... The challenge comes in part because they store their DNA. inside a protective sac, the nucleus, just as human cells do. ... the most common fungal cause of respiratory illness in people in the United States. Given ... Worldwide, millions of people suffer debilitating and even fatal fungal infections.. People with weakened immune systems, such ...
PathogensFungiInfectionsSpeciesBacterial and fungalPathogenSporesConducting molecularSequencesAspergillusProteinsIsolatesYeastDiseasesCandidaTaxonomyInfectionSoil fungalStrains2022VirulenceGeneAmplificationGenomeBiodiversityInvasiveFindings suggest that fungalRibosomalGenesGenomicFusariumDiversityCommunity compositionDetectionShifts in fungalAmplifyEpidemiologyGenomesFound fungalMicrobiotaCommon fungalContact lens wearersCompositionSpecimensPolymerase chain reMorphologyResearchersCommunitiesSequencePhylogenyInteractionsMorphogenesisTaxaFungusGenomicsMicroorganismsTaxonomicBiomassYeastsGrowthCellsPlantBiologicalBiotechnologyPurification
Pathogens19
- However, their application to complex fungal pathogens has remained limited due to the frequent inability to culture these pathogens in the absence of their host and their large genome sizes. (biomedcentral.com)
- Here, we describe the development of Mobile And Real-time PLant disEase (MARPLE) diagnostics, a portable, genomics-based, point-of-care approach specifically tailored to identify individual strains of complex fungal plant pathogens. (biomedcentral.com)
- [ 1 , 2 ] That such contact only rarely results in infection is a testimony to the efficiency with which human immune defenses recognize and eliminate fungal pathogens. (medscape.com)
- Using DNA sequencing-based diagnostics, the results herein document the first identification and quantification of significant bacterial, rather than mycotic, pathogens to the clinical manifestation of dystrophic nails. (japmaonline.org)
- Detection of airborne propagules of fungi, plant pathogens etc (e.g. sporangia of the potato late blight pathogen Phytophthora infestans ) and pollen using novel air sampling devices, flow cytometry and DNA metabarcoding. (aber.ac.uk)
- Full taxonomic characterisation of fungal communities is necessary for establishing ecological associations and early detection of pathogens and invasive species. (pensoft.net)
- Here we link metabarcoding from the ITS2 and LSU D1-D2 regions to characterise fungal communities associated with bark beetles ( Scolytinae ), the likely vectors of several tree pathogens. (pensoft.net)
- This review summarizes the epidemiological and clinical data of the majority of healthcare-associated outbreaks reported over the last 6 years caused by uncommon or new fungal pathogens, as well as the contribution of WGS as support to investigate the source of infection and the most frequent control measures used. (biomedcentral.com)
- Since many publications have so far been interested in classical fungal infections, we aim to decipher the most notable healthcare-associated outbreaks caused in the last 6 years by uncommon or new fungal pathogens (summarized in Table 1 ). (biomedcentral.com)
- Hyphal malformation was also observed in both of the fungal pathogens. (tubitak.gov.tr)
- We're able to detect viruses, bacteria and fungal pathogens that are found in bees, that are also found in the honey," said Bateson. (agweek.com)
- Zack Bateson lifts a honey sample from a hive near Barnesville, Minnesota, to be tested for the DNA of pathogens that cause diseases, which can contribute to the death of a bee colony. (agweek.com)
- The possible mechanisms of the joint action of thiosulfonates and biosurfactants on fungal pathogens are proposed. (intechopen.com)
- Dynamics of transposable elements in recently diverged fungal pathogens: lineage-specific transposable element content and efficiency of genome defences. (mpg.de)
- In the case of opportunistic fungal pathogens, an understanding of host immune dysfunction is as important as an appreciation of which fungal genes may encode definable attributes of virulence. (biomedcentral.com)
- But the rapid progress made in identifying molecular virulence factors in several fungal pathogens has also raised some caveats and paradoxes that require resolution. (biomedcentral.com)
- capsulatum is a dimorphic fungus whose conidia (spores) are inhaled, germinate in the lungs and convert to a budding yeast form that, uniquely among fungal pathogens, becomes an intracellular parasite that is ingested by pulmonary macrophages but survives and multiplies within phagolysosomes. (biomedcentral.com)
- The versatility of targeted amplicon deep sequencing (TADS) enables detection of new pathogens as well as known pathogens that are difficult-to-culture and has facilitated the detailed characterization of bacterial, viral, and fungal communities from diverse biological and environmental specimens. (biomedcentral.com)
- Laboratory sections serve as international resources to identify and characterize bacterial and fungal pathogens. (cdc.gov)
Fungi34
- Fungal DNA barcoding is the process of identifying species of the biological kingdom Fungi through the amplification and sequencing of specific DNA sequences and their comparison with sequences deposited in a DNA barcode database such as the ISHAM reference database, or the Barcode of Life Data System (BOLD). (wikipedia.org)
- In this attempt, DNA barcoding relies on universal genes that are ideally present in all fungi with the same degree of sequence variation. (wikipedia.org)
- Fungal DNA barcoding can help to identify and associate anamorphic and teleomorphic stages of fungi, and through that to reduce the confusing multitude of fungus names. (wikipedia.org)
- The success of identification of fungi by means of DNA barcode sequences stands and falls with the quantitative (completeness) and qualitative (level of identification) aspect of the reference database. (wikipedia.org)
- 2012) proposed the ITS region as primary DNA barcode region for the genetic identification of fungi. (wikipedia.org)
- This document describes some of the target genes and primers that can be used for DNA sequence-based identification of fungi and the PCR conditions with which to use those primers. (cdc.gov)
- DNA from contaminating external fungi should be eliminated, if possible, prior to determining if there are endosymbiotic fungi associated with a laboratory colony of the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Psyllidae). (bioone.org)
- Fungi were less diverse but most dissimilar in composition in the most water-damaged classrooms compared to the least water-damaged, indicating differential effects of individual classroom water-damage on fungal compositions. (cdc.gov)
- The foresters have sent samples of soil from their plots to the researchers, who use DNA sequencing to determine which fungi are present in the soil. (insidescience.org)
- Our result reveal a comprehensive understanding of fungal community structure of natural O. sinensis , provide new insight into O. sinensis associated fungi, and support that microbiota of natural O. sinensis is an untapped source for novel bioactive metabolites discovery. (biomedcentral.com)
- The ancestor of termites relied on gut symbionts for degradation of plant material, an association that persists in all termite families.1,2 However, the single-lineage Macrotermitinae has additionally acquired a fungal symbiont that complements digestion of food outside the termite gut.3 Phylogenetic analysis has shown that fungi grown by these te. (researchgate.net)
- Conventional studies of fungal communities have been conducted by isolating and culturing the fungi associated with insect specimens ( Batra 1963 ), but this overlooked many unculturable species. (pensoft.net)
- In order to address mechanisms responsible for fungal dispersal, this study will focus on determining which species, population and environmental characteristics correlate with dispersal inferred from population genotype patterns throughout a community of fungi. (europa.eu)
- To monitor the effect of land use change, different fungal parameters such as richness, diversity, community composition, root colonization by arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) fungi, spore density, ergosterol, and phospholipid fatty acid (PLFA) content and AM fungal glomalin related soil protein (GRSP) were studied. (sisef.it)
- Given this data, we would hypothesize that: (i) the evolution of the fungal PPIases is driven, at least in part, by the size of the proteome, (ii) evolutionary pressures differ both between the different PPIase families and the different fungi, and (iii) whilst the cyclophilins and parvulins have evolved to perform conserved functions, the FKBPs have evolved to perform more variable roles. (biomedcentral.com)
- Usually Aspergillus and Candida are fungi that cause major fungal infection leading to mortality amongst immunocompromised patients ( 10 ). (yeastinfectionadvisor.com)
- By comparing the DNA sequences of these fungi to those of other species around the world, the team was also able to piece together where the fungal biodiversity on the remote island may have originated. (berkeley.edu)
- A further aim is to promote fungal taxonomy by employing a polythetic approach to clarify the true phylogeny and relationships within the kingdom Fungi. (ingentaconnect.com)
- But as an editorial in the journal pointed out, this sample represented one of many "dark matter fungi" that populate unknown regions of the fungal tree of life. (lanl.gov)
- A massive genetic survey of the world's fungi - an often-overlooked group of organisms with important ties to human health and the economy - has revealed the main drivers of fungal diversity. (aaas.org)
- But, some fungi defy this trend and, in general, the overall number of fungal species doesn't decline with latitude as sharply as plant species do, they say. (aaas.org)
- Our study shows that it's only possible to assign a species name to 10% of the fungi found in soil, indicating how little we know about fungal diversity," said Tedersoo. (aaas.org)
- DNA methylation is an important epigenetic control mechanism that in many fungi is restricted to genomic regions containing transposable elements (TEs). (biomedcentral.com)
- Two DNA methyltransferases, Dim2 and Dnmt5, are known to perform methylation at cytosines in fungi. (biomedcentral.com)
- In mammals, DNA methylation is largely restricted to CG sites, while plants and fungi show methylation in each of the genomic contexts [ 8 ]. (biomedcentral.com)
- Compared to animal and plant genomes, fungi typically have smaller and less complex genomes, and they serve as important eukaryote models for various cellular processes including DNA methylation [ 9 ]. (biomedcentral.com)
- Much of the initial research on DNA methylation in fungi was performed in the saprophytic ascomycete fungus Neurospora crassa . (biomedcentral.com)
- Molecular biotyping, epidemiology and fungal susceptibilities of opportunistic human pathogenic fungi, ICMR , (2002-2005). (jnu.ac.in)
- Sophisticated molecular biological research has revealed many virulence attributes in at least four pathogenic fungi, but the future study of fungal virulence requires investigators to distinguish between molecules that directly interact with the host, molecules that regulate these, and molecules that are always required for fungal growth and survival, independent of the host. (biomedcentral.com)
- Almost all of the fungi that cause fatal diseases gain access to the host via the lungs by inhalation of spores, yet Candida species, which account for a high morbidity and mortality in individuals with reduced numbers of neutrophils, become pathogenic only when lowered host defences allow the fungal cells to pass from the gut into the bloodstream. (biomedcentral.com)
- 2010. ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases. (unimilitar.edu.co)
- Additionally, multiple fungal-bacterial-immune ecologies were detected across tumors, and intratumoral fungi stratified clinical outcomes, including immunotherapy response, they noted. (medscape.com)
- A revised version of Chapter F of the International Code of Nomenclature for algae, fungi, and plants is presented, incorporating amendments approved by the Fungal Nomenclature Session of the 11th International Mycological Congress held in San Juan, Puerto Rico in July 2018. (biomedcentral.com)
- As specified by Division III of the Shenzhen Code , participation in the guiding vote was extended to (1) authors of proposals, (2) members of the Nomenclature Committee for Fungi (NCF), (3) members of the International Mycological Association (IMA), and of its Member Mycological Organizations (MMOs), and (4) members of four additional organizations that were nominated by the Fungal Nomenclature Bureau. (biomedcentral.com)
Infections24
- Blindness due to corneal infections is a serious problem next to cataract [ 1 ] and fungal infections of the cornea have emerged as a major eye disease globally. (hindawi.com)
- The serum CrAg-latex agglutination the fungal infections and preserve engraftment. (cdc.gov)
- Invasive fungal diseases are increasingly important opportunistic infections that are intimately linked to immune-suppression in the context of cytotoxic treatment of neoplastic diseases, stem cell and solid-organ transplantation, and primary immune deficiencies. (medscape.com)
- Consequently, invasive fungal infections almost invariably signify an immunocompromised state. (medscape.com)
- on the one hand, the availability of advanced diagnostic tools, including high-end imaging modalities such as high-resolution computerized tomography and MRI, as well as nonculture-based fungal detection assays, allow these infections to be diagnosed earlier than was previously possible. (medscape.com)
- However, despite these advances, mortality rates from invasive fungal infections remain extremely high, suggesting that the underlying immune deficiency must be addressed to facilitate further reductions in fungal-related mortality. (medscape.com)
- On June 3, a local physician notified the Springfield-Greene County Health Department and the Missouri Department of Health and Senior Services (MODHSS) of two patients hospitalized with tornado injuries who had suspected necrotizing fungal soft-tissue infections. (cdc.gov)
- MODHSS initiated active surveillance for such infections at hospitals and laboratories serving patients injured in the tornado, and CDC began assisting MODHSS with identification of fungal isolates. (cdc.gov)
- By June 10, eight patients with necrotizing fungal soft-tissue wound infections caused by Mucormycetes (formerly Zygomycetes) were identified. (cdc.gov)
- Worldwide, millions of people suffer debilitating and even fatal fungal infections. (sciencenews.org)
- In this study, the applicability of such assays for the detection of fungal infections in grape, soil, and must samples was tested and optimized. (infowine.com)
- The application of LAMP assays for the detection of fungal infections prior to the occurrence of visual mold symptoms by testing samples from vineyards is particularly beneficial. (infowine.com)
- Outbreaks of healthcare-associated fungal infections have repeatedly been described over recent years, often caused by new or uncommon species. (biomedcentral.com)
- Furthermore, these outbreaks identified new populations under threat in addition to those commonly at risk for invasive fungal infections, such as immunosuppressed and intensive care unit patients. (biomedcentral.com)
- In fact, thousands of deaths can be traced every year to fungal infections around medical implants. (mit.edu)
- The resulting immune system abnormalities make people with AD-HIES highly susceptible to infections, particularly bacterial and fungal infections of the lungs and skin. (medlineplus.gov)
- Illness in a person with compelling evidence (e.g., culture, histopathology, seroconversion) of a different fungal infection, such as histoplasmosis or coccidioidomycosis, and meeting only non-confirmatory laboratory criteria for blastomycosis should not be counted as a case of blastomycosis since other fungal infections can cause false positive Blastomyces antigen and antibody test results. (cdc.gov)
- The fungal superbug Candida auris causes serious and often fatal infections. (cdc.gov)
- So generally, as you all know who work in healthcare and see infections, you know that most of the time the public thinks of fungal disease as what is shown in the picture here. (cdc.gov)
- So this is a classic, very advanced case of onychomycosis and clearly probably the most common clinical manifestation of fungal infections out there. (cdc.gov)
- But, thankfully, as bad as these fungal infections look they aren't deadly and what we are obviously concerned about is deadly infections. (cdc.gov)
- Candidemia, which is the bloodstream manifestation of fungal infections due to Candida , turns out in recent studies that we've looked at are the most common if not some of the most common healthcare associated bloodstream infections in our country, in the United States. (cdc.gov)
- The risk factors for candid are sometimes we call in the fungal world the other C. diff , have somewhat similar characteristics in that broad spectrum antibacterial use is a very common association with those who develop infections due to Candida . (cdc.gov)
- Athlete's Foot or tinea pedis, and fungal nail infections, also known as onychomycosis or tinea unguium. (cdc.gov)
Species33
- The interspecific variation, i.e., the variation between species, in the chosen DNA barcode gene should exceed the intraspecific (within-species) variation. (wikipedia.org)
- Moreover, fungal species can comprise multiple strains that can vary in their morphology or in traits such as carbon- and nitrogen utilisation, which has often led to their description as different species, eventually producing long lists of synonyms. (wikipedia.org)
- Trichophyton species DNA is amplified very poorly by the ITS primer set used for most other molds. (cdc.gov)
- Both serological and DNA-based methods typically require high initial financial investments and specialised expertise to develop new assays, are limited in sample capacity, frequently are not reliable at the asymptomatic stage, and provide limited information beyond the species level [ 1 ]. (biomedcentral.com)
- It is demonstrated that even small samples of seawater contain eDNA from a wide range of local fish species, which indicates the potential of using metabarcoding of environmental DNA (eDNA) obtained directly from seawater samples to account for marine fish biodiversity. (semanticscholar.org)
- Environmental DNA for Detection of Endangered Grouper Species (Epinephelus spp. (semanticscholar.org)
- The Department of Energy's Joint Genome Institute has a library of genomes for well over a thousand fungal species, which Averill's team used to understand the functional genetics of the samples. (insidescience.org)
- The fungal species (Jugulospora rotula and Emericellopsis terricola in NPK and NPKM soils, respectively) were identified as keystone species in network and may significantly contribute to residue-C decomposition. (olmdiagnostics.com)
- Metabarcoding is now widely applied in characterising the species composition and diversity of fungal communities associated with insects. (pensoft.net)
- However, molecular techniques, such as DNA sequencing, should be used to identify Aspergillus species in cases that involve either isolates with atypical growth or concern for resistance. (medscape.com)
- Also, large nosocomial outbreaks due to uncommon fungal species such as Exserohilum rostratum and Sarocladium kiliense , were both linked to contamination of medical products, however the source of another outbreak, caused by Saprochaete clavata , remains unresolved. (biomedcentral.com)
- The occurrence of sporadic episodes related to other fungal species and other less immunocompromised populations remained a rare phenomenon. (biomedcentral.com)
- Nevertheless, several recent reports have highlighted the emergence of new healthcare-associated outbreak phenomena due to fungal species previously unknown or uncommon in clinical practice. (biomedcentral.com)
- Also, others healthcare-associated outbreaks due to uncommon fungal species, such as Saprochaete clavata, are still unresolved as no source of contamination has yet been documented. (biomedcentral.com)
- The kits isolate both genomic DNA and a broad range of RNA species without the use of phenol. (selectscience.net)
- Unification of the methodology, detailed descriptions of environmental factors, more reference sequences in public databases, and especially data on ecology and quantitative parameters of key fungal species would significantly improve the understanding of this issue. (sisef.it)
- Chaetomium are a common fungal species with a ubiquitous distribution with over 80 species currently known. (mold-help.org)
- At first we will of course not be able to identify all the fungal species, because they have not yet been described scientifically. (helsinki.fi)
- Our project will reveal which fungal families have the greatest numbers of undescribed species, where they are in the world, and where we need additional taxonomic research the most. (helsinki.fi)
- Recent studies on the fungal families Lophiostomataceae and Lophiotremataceae ( Pleosporales ) have provided varying phylogenetic and taxonomic results concerning constituent genera and species. (ingentaconnect.com)
- They used a DNA sequencing technique, known as pyrosequencing, to study a short genetic marker within the myriad of fungal species that these samples contained. (aaas.org)
- It was assumed that plant and fungal species richness are tightly coupled. (aaas.org)
- The researchers' vast study also highlights the gap between known and described fungal species, suggesting that previous studies may have overestimated fungal richness. (aaas.org)
- This devastating fungal infection has not yet arrived in Australia, but is sweeping Europe and the US and threatens several of our bat species with extinction if it should reach these shores. (edu.au)
- as well as several fungal species were isolated from different grains. (scialert.net)
- Cortesão investigates fungal species' capacity to grow in the conditions of space with the aim of harnessing the microorganisms as biological factories for materials people might need on long space voyages. (eurekalert.org)
- Pathogenic fungal species differ in their cell morphologies: most exist as filamentous moulds (with hyphae) in the environment, but the most virulent among them often change to a unicellular (yeast or spherule) form when they invade tissues. (biomedcentral.com)
- The diversity of pathogenic potency, cellular form and route of invasion between fungal species makes it impossible to draw general conclusions about their molecular virulence attributes. (biomedcentral.com)
- An integrated molecular and morphological study to design a DNA barcode discrimination protocol for Fusarium species involved in dry root disease of citrus. (unimilitar.edu.co)
- 2009. Multiple copies of cytochrome oxidase 1 in species of the fungal genus Fusarium. (unimilitar.edu.co)
- The internal transcribed spacer (ITS) region of nuclear ribosomal DNA is the most frequently used marker for distinguishing plant species. (academicjournals.org)
- Diagnosis based solely on fungal morphology is unreliable due to the ubiquitous nature of non-pathogenic Fusarium species. (agdia.com)
- This taxon is best considered a distinct species, as it was not recovered as part of the S. graminea species clade based on a DNA phylogeny (Ito et al. (usf.edu)
Bacterial and fungal3
- LOOXSTER sample preparation kit concentrates bacterial and fungal DNA! (cbinsights.com)
- DNA testing can determine the viral, bacterial and fungal disease load carried by a bee. (agweek.com)
- Under laminar flow, a suspension of respective bacterial and fungal cells (20 billion cells/mL) was processed as per the Homoeopathic Pharmacopoeia of India (HPI). (thieme-connect.de)
Pathogen7
- However, molecular-age medicine now includes DNA-based tools to universally assess any microbe or pathogen with a known DNA sequence. (japmaonline.org)
- Bateson is now using the same process to track pathogen DNA in honey samples. (agweek.com)
- Palka-Santini M, Cleven BE, Eichinger L, Krönke M, Krut O. Large scale multiplex PCR improves pathogen detection by DNA microarrays. (aaem.pl)
- Recent loss of the Dim2 DNA methyltransferase decreases mutation rate in repeats and changes evolutionary trajectory in a fungal pathogen. (mpg.de)
- A fungal pathogen induces systemic susceptibility and systemic shifts in wheat metabolome and microbiome composition. (mpg.de)
- The transcription factor Zt107320 affects the dimorphic switch, growth and virulence of the fungal wheat pathogen Zymoseptoria tritici. (mpg.de)
- It is certainly an honor and a pleasure to be here during our first inaugural Fungal Disease Awareness Week, and I look forward to talking to you a little bit about an emerging fungal pathogen that is really causing some consternation and challenges amongst the healthcare community. (cdc.gov)
Spores7
- Although cutaneous mucormycosis often is opportunistic, affecting patients with diabetes, hematologic malignancy or solid organ transplant ( 1 ), A. trapeziformis often is associated with immunocompetent hosts after traumatic implantation of fungal spores ( 2 ). (cdc.gov)
- The vortex that forms in the machine separates small particles such as fungal spores into a test tube. (helsinki.fi)
- The disease spreads when fungal spores blow from an infected tree onto an uninfected specimen, which they invade. (thenakedscientists.com)
- We now know that [fungal spores] resist radiation much more than we thought they would, to the point where we need to take them into consideration when we are cleaning spacecraft, inside and outside," Cortesao said. (eurekalert.org)
- The new research also suggests planetary protection protocols designed to prevent visiting spacecraft from contaminating other planets and moons in our solar system with microorganisms from Earth may need to consider fungal spores a more serious threat. (eurekalert.org)
- Cortesão simulated space radiation in the laboratory, hitting fungal spores with ionizing radiation from X-rays, heavy ions and a type of high-frequency ultraviolet light that doesn't reach Earth's surface but is present in space. (eurekalert.org)
- Information obtained from the analysis of dust, particularly biological particles such as pollen, plant parts, and fungal spores, has great utility in forensic geolocation. (mit.edu)
Conducting molecular1
- To date, no consistent method has been developed to eliminate microbial DNA from the external surfaces of arthropods prior to conducting molecular surveys of endosymbionts. (bioone.org)
Sequences11
- The region is flanked by the DNA sequences for the ribosomal small subunit (SSU) or 18S subunit at the 5' end, and by the large subunit (LSU) or 28S subunit at the 3' end. (wikipedia.org)
- Genomic DNA from dust was extracted for internal transcribed spacer 1 Illumina MiSeq sequencing to identify operational taxonomic units (OTUs) organized from DNA sequences. (cdc.gov)
- We identified 62 yeast genera, representing 19.6% of DNA sequences. (cdc.gov)
- A total of 280,519 high-quality sequences, belonging to 5 fungal phyla, 15 classes, 41 orders, 79 families, 112 genera, and 352 putative operational taxonomic units (OTUs) were obtained from natural O. sinensis using high-throughput sequencing. (biomedcentral.com)
- Loop-mediated isothermal amplification (LAMP) [1] is a modern technology for fast and sensitive amplification of specific DNA sequences under isothermal conditions. (infowine.com)
- It accounted for over 50% of fungal DNA sequences sampled from the filter and was found repeatedly in suspect air. (scientificamerican.com)
- The fungal isolates were identified by employing morphological features and phylogenetic characterization by internal transcribed spacer (ITS) sequences. (researchsquare.com)
- By adding DNA sequences of 24 new strains of Lophiostomataceae and nine new strains of Lophiotremataceae to a sequence data matrix from international databases, we provide a new understanding of the relationships within these families. (ingentaconnect.com)
- Data partitions, Bayesian analysis and phylogeny of the zygomycetous fungal family Mortierellaceae, inferred from nuclear ribosomal DNA sequences. (mpg.de)
- To find out what those genes are, UK scientists have compared the DNA sequences of susceptible and more resilient ash trees. (thenakedscientists.com)
- Evolutionary trends from DNA sequences: A maximun likelihood approach. (unimilitar.edu.co)
Aspergillus7
- More importantly, Fusarium and Aspergillus are reported commonly implicating corneal ulcer and against this background the present work was undertaken so as to understand the current epidemiological trend of the two fungal keratitis. (hindawi.com)
- Among fungal aetiologies, Fusarium (216, 52.5% of 411) and Aspergillus (68, 16.5% of 411) were predominantly determined. (hindawi.com)
- In South India, the dominance of fungal keratitis, particularly of Fusarium and Aspergillus, is prevalent more than a decade and have been documented in many literatures [ 5 - 8 ]. (hindawi.com)
- For example, the filamentous fungus Aspergillus fumigatus , an important cause of invasive fungal disease in immunosuppressed individuals, disperses conidia that are inhaled daily by most humans. (medscape.com)
- Most of fungal genera in NPKM soils, especially Chaetomium, Staphylotrichum, Penicillium and Aspergillus, responded faster to residue addition than those in NPK soils. (olmdiagnostics.com)
- The guidelines recommend that both surgery and either systemic voriconazole or a lipid formulation of amphotericin B be used in invasive Aspergillus fungal sinusitis, but surgical removal alone can be used to treat an Aspergillus fungal ball of the paranasal sinus. (medscape.com)
- Among the fungal isolates, Aspergillus sp. (researchsquare.com)
Proteins3
- This process does not require RecA proteins that mediate DNA homology recognition during recombination. (pasteur.fr)
- Bacterial activity (measured as incorporation of [3H]thymidine and [14C]leucine into bacterial DNA and proteins) showed the exact opposite: an increase with N application rate and pH. (louisbolk.nl)
- Quantitative proteomic analysis of yeast DNA replication proteins. (abdn.ac.uk)
Isolates5
- Although voriconazole and other triazoles have broad-spectrum activity against causative fungal isolates, clinically no single drug was found to be effective against fungal keratitis. (hindawi.com)
- The antioxidant activity methanolic extracts of the fungal isolates were determined using modified 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) methods. (researchsquare.com)
- Our findings suggest that fungal isolates from D. metel can be a sustainable resource for natural antioxidants. (researchsquare.com)
- These endophytic and soil fungal isolates were found to be significantly active by inhibiting the DPPH, ABTS radicals and showed active spots on the DPPH bio - autography assay. (researchsquare.com)
- It is believed that these radical anions interfere with bacterial DNA synthesis of susceptible isolates. (medscape.com)
Yeast6
- Histopathology of microbiological remission of the infection, dos- the graft showed organisms consistent with Crypto- age was adjusted on the basis of renal function coccus yeast cells, suggesting fungal pyelonephritis. (cdc.gov)
- In this study, the researchers found that baker's yeast (Saccharomyces cerevisiae) adhered to plastic plates, suggesting that like their infectious fungal cousins, yeast, too, can form biofilms. (mit.edu)
- Solution 1 Digestion Buffer is used with Zymolyase to breaks down tough yeast and fungal cell walls for DNA purification. (zymoresearch.com)
- Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. (cdc.gov)
- Clotrimazole is a broad-spectrum antifungal agent that inhibits yeast growth by altering cell membrane permeability, causing the death of fungal cells. (medscape.com)
- Genome-wide expression profiling, in vivo DNA binding analysis, and probabilistic motif prediction reveal novel Abf1 target genes during fermentation, respiration, and sporulation in yeast. (yeastgenome.org)
Diseases7
- Summary of evidence for various immunotherapeutic modalities for invasive fungal diseases. (medscape.com)
- Pathogenomics-based Development of Crop-specific Diagnostics Tools for Emerging and Expanding Fungal Diseases in the U.S. (usda.gov)
- Eutypa dieback and Esca are serious fungal grapevine trunk diseases (GTDs). (usda.gov)
- Pests and fungal diseases are on the march. (brinknews.com)
- Perception of the high incidence of fungal diseases, particularly those that threaten life, has led to considerable investment in research into fungal virulence, often based on cutting-edge molecular biological approaches. (biomedcentral.com)
- Dr. Chiller is an Infectious Disease Physician, who has specialized in fungal diseases for the past 20 years. (cdc.gov)
- He leads CDC's efforts to combat fungal diseases as the Chief of the Mycotic Diseases Branch in the Office of Emerging Infectious Diseases in the National Center for Emerging and Zoonotic Infectious Diseases at CDC. (cdc.gov)
Candida1
- Fungal genomics: decoding the secrets of Candida tropicalis - in which Dr Caitlin Keighley describes the application of genomics to unravelling the reasons for the emergence of fluconazole resistance in C. tropicalis in Australia and in many regions globally. (edu.au)
Taxonomy2
- LOS ALAMOS, N.M., Sept. 28, 2017-In a step toward bridging the gap between fungal taxonomy and molecular ecology, scientists from several institutions including Los Alamos National Laboratory have characterized a sample of "mystery" fungus collected in North Carolina and found its home in the fungal tree of life. (lanl.gov)
- DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. (unimilitar.edu.co)
Infection6
- The corneal infection of fungal etiology is very common and comprising at least 50% of all culture positive cases in India [ 2 ]. (hindawi.com)
- A confirmed case was defined as 1) necrotizing soft-tissue infection requiring antifungal treatment or surgical debridement in a person injured in the tornado, 2) with illness onset on or after May 22, and 3) positive fungal culture or histopathology and genetic sequencing consistent with a Mucormycete. (cdc.gov)
- Is cancer really just a fungal infection? (drhoffman.com)
- David Phalen returns to discuss his investigations into a fungal infection of wombats in this talk Emmonsielloposis in a Fossorial Australian Marsupial. (edu.au)
- A fungal infection can lead to complications for contact lens wearers and, more seriously, patients with hip replacements. (mit.edu)
- Cross-reactivity is a known problem with the EIA antigen test, and cases known to be infected with another fungal infection should not be counted as blastomycosis cases. (cdc.gov)
Soil fungal3
- Soil fungal communities across land use types. (sisef.it)
- Belowground fungal diversity is very important in terrestrial ecosystems, however, the effect of land use change on soil fungal community is poorly understood. (sisef.it)
- It is frequently hypothesised that high soil fungal/bacterial ratios are indicative for more sustainable agricultural systems. (louisbolk.nl)
Strains1
- We isolated 23 fungal strains on inflorescences and tubers from three B. japonica plants at two locations and on one sample of the host plant ( Symplocos lancifolia , Symplocaceae). (scirp.org)
20221
- Ceballos-Escalera A, Richards J, Arias MB, Inward DJG, Vogler AP (2022) Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU) rRNA markers. (pensoft.net)
Virulence2
- Single or double DNA methyltransferase mutants did not show altered development, virulence, or transcription of genes or TEs. (biomedcentral.com)
- In contrast, Hp1 and Dim5 mutants that are impacted in chromatin-associated processes upstream of DNA methylation are severely affected in development and virulence and display transcriptional reprogramming in specific hypervariable genomic regions (so-called adaptive genomic regions) that contain genes associated with host colonization. (biomedcentral.com)
Gene8
- Coprinopsis cinerea is easily transformed by DNA, and targeted gene disruption is achieved in some instances. (broadinstitute.org)
- A chromosomal gene was found to be involved in the production of a fungal antagonistic compound as demonstrated by the gel elution technique using antibiotic gene-specific primers. (tubitak.gov.tr)
- Quantitative monitoring of gene expression patterns with a complementary DNA microarray. (aaem.pl)
- The gene, called FLO11, is required for fungal biofilm formation. (mit.edu)
- The results revealed that the first strain exhibited a high level of 18S rRNA similarity (99%) with Fusarium verticillioides isolate (GenBank accession No. KJ207389.1), whereas, the second fungal strain of the sequenced 18S rRNA gene was identified as a close relative (99%) to Fusarium sp. (scialert.net)
- The partial or total sequencing of the 18S ribosomal DNA (rRNA) gene showed a fast technique for fungal classification. (scialert.net)
- 2011. Altenative oxidase gene (AOX I): a good DNA barcoding candidate for the genus Fusarium. (unimilitar.edu.co)
- They touted a "scalable" technology, named in shorthand FAC-MS , that can sift through important chunks of fungal DNA-what are known as gene clusters-to find molecules that might be useful for drugmaking. (xconomy.com)
Amplification6
- Loop-mediated isothermal amplification of DNA. (infowine.com)
- Thus, the plant-specific ITS primer design is required to achieve the preferential amplification of plant DNA with discrimination against fungal DNA from environmental plant samples. (academicjournals.org)
- For P. tongkangensis , the dismatch of ITS5 primer resulted in noneffective or wrong amplification when paired with either ITS4-R or ITS4, while ITS1-F2, when paired with ITS4-R and ITS4, efficiently amplified plant DNA and both plant and fungal DNAs, respectively. (academicjournals.org)
- Meanwhile, universal amplification of conserved loci from all parasites without amplifying host DNA has proven challenging. (biomedcentral.com)
- This nested approach facilitated introduction of a second restriction digestion between the first and second PCR, reducing the proportional mass of amplifiable host-derived DNA while increasing the number of PCR amplification cycles. (biomedcentral.com)
- This technology promotes the rapid amplification and detection of nucleic acid targets, DNA or RNA, while maintaining a single operating temperature of 39 - 42 o C. The AmplifyRP® XRT products achieve target sensitivity and specificity comparable to PCR, while having clear advantages over the lab-based technology. (agdia.com)
Genome6
- The ability of RIP to detect any two identical DNA segments placed at arbitrary genomic positions suggests that RIP involves an exhaustive, "genome-by-genome" homology search. (pasteur.fr)
- PPIase numbers within these fungal repertoires appears associated with genome size and orthology between repertoires was found to be low. (biomedcentral.com)
- Also, the repertoire of Cryptococcus neoformans may represent a better model fungal system within which to study the functions of the PPIases as its genome size and genetic tractability are equal to those of Saccharomyces cerevisiae , whilst its repertoires exhibits greater orthology to that of humans. (biomedcentral.com)
- The Coprinopsis cinerea sequence project is part of the Broad Institute's Fungal Genome Initiative. (broadinstitute.org)
- Genomic DNA for the genome project was provided by Patricia Pukkila at University of North Carolina. (broadinstitute.org)
- It has a long road ahead to prove that it can analyze a fungal genome and accurately predict which genes will yield potential drugs. (xconomy.com)
Biodiversity8
- Westerdijk Fungal Biodiversity and mycophenolic acid. (cdc.gov)
- PDF] Monitoring endangered freshwater biodiversity using environmental DNA. (semanticscholar.org)
- It is argued that the proposed DNA‐based approach has the potential to become the next‐generation tool for ecological studies and standardized biodiversity monitoring in a wide range of aquatic ecosystems. (semanticscholar.org)
- This current project is the first to integrate community, population and genotype-level information which will help to gain a clear picture of fungal dispersal and how it influences biodiversity. (europa.eu)
- Better understanding of the mechanisms will enhance the ability to manage and conserve the biodiversity and functions of fungal communities in forest world-wide. (europa.eu)
- It's truly uncharted territory in evolutionary biology and biodiversity of the fungal kingdom, and this is one the first attempts to generate baseline information on fungal diversity, not just for Mo'orea, but for the entire and vast Insular Oceania region. (berkeley.edu)
- Persoonia is a journal published jointly by the Naturalis Biodiversity Center and the Westerdijk Fungal Biodiversity Institute. (ingentaconnect.com)
- Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring. (unimilitar.edu.co)
Invasive1
- Surgery for aspergillosis should be considered for localized disease that is easily accessible to debridement (eg, invasive fungal sinusitis, localized cutaneous disease). (medscape.com)
Findings suggest that fungal1
- These findings suggest that fungal activity is "a new and emerging hallmark of cancer," stated study co-leader Ravid Straussman, PhD, of the Weizmann's Molecular Cell Biology Department. (medscape.com)
Ribosomal1
- BeCHAPTER cause the highly conserved ribosomal genes flanking the ITS1 marker may distort sequence clustering and similarity searches, we removed these from the dataset using Fungal ITS Extractor 1.1 (Nilsson et al. (bestpfe.com)
Genes8
- Such analyses will help to address questions such as whether the genes responsible for the multicellularity of C. cinerea are the progenitors of those in other multicellular organisms, or are innovations restricted to the fungal kingdom. (broadinstitute.org)
- Genomic analysis may reveal whether the genes responsible for the multicellularity of Coprinopsis cinerea are the progenitors of those in other multicellular organisms (e.g., nuclear receptors) or are innovations restricted to the fungal kingdom. (broadinstitute.org)
- I think their aim is to clone random genes from an animal cell, using 'quick-and-easy' DNA extracts. (hackaday.com)
- I reckon they should add more genes that you can add directly instead of waiting on stretch goals, or just adding a whole palette of genes and getting rid of the "extract your own" DNA part. (hackaday.com)
- We highlight possible strategies for unlocking lead genes for rational strain optimizations based on omics data, and discuss how targeted genetic manipulation of these candidates can be used to optimise fungal morphology for improved performance. (biomedcentral.com)
- The researchers are now using DNA arrays -- DNA chips that can analyze the activity of thousands of genes simultaneously -- to understand how FLO11 works in conjunction with other genes to bring about this effect. (mit.edu)
- Estos métodos incluyen la construcción de árboles de genes, determinación del "barcode gap", estimación de índice de clasificación genealógica, análisis de variabilidad génica, redes de haplotipos, DNA-BAR y BLOG. (unimilitar.edu.co)
- To carry out its roles, the STAT3 protein attaches to DNA and helps control the activity of particular genes. (medlineplus.gov)
Genomic6
- The Quick-DNA/RNA™ kits provide a quick method for the isolation of high quality genomic DNA and total RNA from cells, tissue, blood, biological fluids, etc. (selectscience.net)
- PCR analysis of the genomic DNA with antibiotic specific primers detected phenazine-1-carboxylic acid, 2,4-diacetylphloroglucinol, and pyoluteorin. (tubitak.gov.tr)
- As these adaptive genomic regions are largely devoid of DNA methylation and of Hp1- and Dim5-associated heterochromatin, the differential transcription is likely caused by pleiotropic effects rather than by differential DNA methylation. (biomedcentral.com)
- Cytosine methylation can occur in symmetric CG or CHG genomic contexts, or in the asymmetric CHH genomic context, where H stands for either A, C or T. In general, 5mC occurs more commonly at symmetric sites because maintenance methylation can cause methylation of daughter strands during DNA-replication, whereas asymmetric sites require de novo methylation [ 7 ]. (biomedcentral.com)
- Dim2 operates in a complex with Heterochromatin Protein-1 (Hp1) that recognizes and directs DNA methylation to genomic regions marked by tri-methylation of histone 3 lysine 9 (H3K9me3) that is deposited by the histone methyltransferase Deficient In Methylation-5 (Dim5) [ 11 , 12 ]. (biomedcentral.com)
- The cost of DNA sequencing has plummeted, and along with it, the ability to store and manage vast amounts of genomic data has increased. (xconomy.com)
Fusarium2
- While the study revealed a male preponderance with both the fungal keratitis , it further brought out that polyene compounds (natamycin and amphotericin B) and azoles were active, respectively, against Fusarium spp. (hindawi.com)
- Agdia, Inc. (Elkhart, IN) is proud to announce the commercialization of a rapid DNA-based assay, on their AmplifyRP® XRT platform, for the detection and identification of Fusarium odoratissimum Tropical Race 4 , formerly known as Fusarium oxysporum formae specialis cubense Tropical Race 4 ( Foc TR4). (agdia.com)
Diversity2
Community composition4
- A preliminary experiment had been performed to assess the effect of oak leaf storage in silica gel on the fungal community composition inferred by 454 pyrosequencing. (bestpfe.com)
- Land use change mostly showed significant shifts in fungal community composition. (sisef.it)
- All these 18 types of land use change influenced fungal community composition, however, the effects on quantitative parameters were mostly inconsistent. (sisef.it)
- In a recent article published in the journal Cell, researchers found fungal deoxyribonucleic acid (DNA) and cells at low abundances across many human cancers, with cancer type-dependent variations in fungus community composition and fungal-bacteriome interactions. (news-medical.net)
Detection2
- A further finding related to the presence of fungal and bacterial DNA in human blood further suggests that measuring microbial DNA in the blood could lead to early detection of cancer, the authors noted. (medscape.com)
- CDC sequenced nearly 45,000 DNA samples by using Advanced Molecular Detection (AMD) technologies. (cdc.gov)
Shifts in fungal1
- To determine the shifts in fungal/bacterial biomass ratio as influenced by grassland management and to find relations with nitrogen leaching potential, we sampled a two-year-old field experiment at an organic experimental farm in the eastern part of The Netherlands. (louisbolk.nl)
Amplify3
- To make those identifications, Jakob uses a backpack-sized laboratory that contains all the equipment needed to extract and amplify fungal DNA, including a microscope and a Polymerase Chain Reaction (PCR) machine. (popsci.com)
- Our results showed that ITS4-R, when paired with either a universal primer ITS5 or the newly designed primer ITS1-F2, efficiently amplified DNA from environmental P. cernua samples and discriminated against parasitic fungal DNAs, while another newly designed primer ITS1-F2, when paired with either a universal primer ITS4 or ITS4-R, could not preferentially amplify plant DNA or discriminate against fungal DNA. (academicjournals.org)
- Pan-eukaryotic PCRs preferentially amplify the more abundant host DNA, obscuring parasite-derived reads following TADS. (biomedcentral.com)
Epidemiology1
- Our core group includes researchers from medical microbiology, clinical research, veterinary agricultural science, environment and life sciences, epidemiology and pharmacy and lead FungiSphere research themes in fungal biology, ecology and epidemiology, food security, pathogenesis and antimicrobial resistance. (edu.au)
Genomes2
- We used targeted sequencing to overcome limitations associated with the size of fungal genomes and their often obligately biotrophic nature. (biomedcentral.com)
- Recent advances in systems-level understanding of the filamentous lifestyle and development of sophisticated synthetic biological tools for controlled manipulation of fungal genomes now allow rational strain development programs based on data-driven decision making. (biomedcentral.com)
Found fungal1
- A separate study , also appearing in Thursday's issue of the journal Cell, found fungal involvement in gastrointestinal and lung tumors. (upi.com)
Microbiota2
- researchers should also pay attention to viruses or fungal members of the microbiota. (microbiomeinsights.com)
- Multi-kingdom microbiota analyses identify bacterial-fungal interactions and biomarkers of colorectal cancer across cohorts. (who.int)
Common fungal2
- for similar vaccines against more common fungal threats, say researchers. (sciencenews.org)
- the most common fungal cause of respiratory illness in people in the United States. (sciencenews.org)
Contact lens wearers1
- The importance of fungal keratitis gained momentum in 2005 following the outbreak of fungal keratitis among contact lens wearers in many developed countries [ 15 , 16 ]. (hindawi.com)
Composition2
- This is connected with the changes in the composition of the rice residue during the degradation and with fungal adaptation (abundance and activity) to continuous manure input. (olmdiagnostics.com)
- Fungal composition revealed in this study differed from the composition on B. harlandii or other root holoparasites with endophytic fungal ( Rafflesia cantleyi ) data. (scirp.org)
Specimens2
- Voucher specimens should be clearly labelled as such, including a permanent voucher identifier that unambiguously connects the specimen with the DNA barcode data derived from it. (wikipedia.org)
- We have really only identified and fully described 100,000 of them, though, and new DNA sequencing capabilities show us that many, many specimens in research collections are uncharacterized. (lanl.gov)
Polymerase chain re3
- Previously, diagnosis had largely been made by means of clinical presentation, although microscopic examinations (potassium hydroxide) of nail scrapings to identify fungal agents and, more recently, panel-specific polymerase chain reaction assays have been used to elucidate causative infectious agents. (japmaonline.org)
- Culture tests, sterility tests and molecular testing (polymerase chain reaction) were performed to establish the absence of contamination, live organisms and DNA material. (thieme-connect.de)
- Previously published polymerase chain reaction (PCR) primers available for amplifying the ITS region from environmental plant samples provide a high degrees of success while maintaining a broad range of compatibility difficult to discriminate against fungal DNA. (academicjournals.org)
Morphology1
- Additionally, fungal signalling cascades are introduced as critical processes that can be genetically targeted to control growth and morphology during biotechnological applications. (biomedcentral.com)
Researchers2
- Researchers at Los Alamos National Laboratory and several other institutions have characterized a sample of "mystery" fungus and found its home in the fungal tree of life. (lanl.gov)
- Annual precipitation appears to be the strongest driver of fungal distribution, according to the researchers. (aaas.org)
Communities9
- We conducted an environmental study to characterize fungal communities in classroom floor dust. (cdc.gov)
- DNA stable-isotope probing delineates carbon flows from rice residues into soil microbial communities depending on fertilization. (olmdiagnostics.com)
- The fungal communities inhabiting natural Ophiocordyceps sinensis play critical ecological roles in alpine meadow ecosystem, contribute to infect host insect, influence the occurrence of O. sinensis , and are repertoire of potential novel metabolites discovery. (biomedcentral.com)
- However, a comprehensive understanding of fungal communities of O. sinensis remain elusive. (biomedcentral.com)
- Therefore, the present study aimed to unravel fungal communities of natural O. sinensis using combination of high-throughput sequencing and culture-dependent approaches. (biomedcentral.com)
- The fungal communities in external mycelial cortices were significantly distinct from soil microhabitat. (biomedcentral.com)
- Here we analyzed bacterial, fungal and ant communities in small road medians (average area 0.0008 km 2 ) and larger parks (average area 0.64 km 2 ) across Manhattan (NYC). (nature.com)
- Studies of fungal communities using the canonical ITS barcode require corroboration across additional loci. (pensoft.net)
- Understanding complex microbial and fungal communities is progressing, Kuske noted, by allowing organisms to grow as defined mixtures. (lanl.gov)
Sequence8
- When the identity of a certain taxon (or a genetic sequence in the case of DNA barcoding) is in doubt, the original specimen can be re-examined to review and ideally solve the issue. (wikipedia.org)
- Like the flanking 18S and 28S subunits, the 5.8S subunit contains a highly conserved DNA sequence, as they code for structural parts of the ribosome, which is a key component in intracellular protein synthesis. (wikipedia.org)
- We are especially interested in understanding cytogenetic phenomena that involve sequence-specific interactions between apparently intact segments of chromosomal DNA. (pasteur.fr)
- Using a genetic process "Repeat Induced Point mutation" (RIP) in Neurospora crassa as a model system, we have discovered that DNA homology can be recognized by a cardinally new mechanism, in which long segments of double-stranded DNA are compared to one another as arrays of short interspersed sequence units. (pasteur.fr)
- The fine-scale structure of both symbionts is not sufficiently resolved by DNA sequence data. (bioone.org)
- The technology of DNA fingerprinting is based on the assumption that no two people have the same DNA sequence. (daleko.space)
- Does not show good practice through the repeating sequence of acetic acid, dna fingerprinting and its application of core loci and this introductory video superimposition technique that eventually identified. (daleko.space)
- Role of multifunctional autonomously replicating sequence binding factor 1 in the initiation of DNA replication and transcriptional control in Saccharomyces cerevisiae. (yeastgenome.org)
Phylogeny1
- 2011. DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus. (unimilitar.edu.co)
Interactions2
- Ongoing advances in our knowledge of fungal-host interactions facilitate rational design of novel immunotherapeutics. (medscape.com)
- Thus, we have proposed that SUV39 methyltransferases can be recruited to repetitive DNA in response to homologous dsDNA-dsDNA interactions. (pasteur.fr)
Morphogenesis1
- Over 100 markers affecting mating type, hyphal growth, fruit body morphogenesis, and DNA repair have been assigned to 10 linkage groups. (broadinstitute.org)
Taxa2
- The Mo'orea BioCode project was the first all-taxa-survey of a tropical island to include DNA vouchers and other associated information. (berkeley.edu)
- The journal publishes high-quality papers elucidating known and novel fungal taxa at the DNA level, and also strives to present novel insights into evolutionary processes and relationships. (ingentaconnect.com)
Fungus5
- A higher fungus (Agaricales), C. cinerea is comprised of many cell types providing a window on the development of multicellularity within the fungal kingdom. (broadinstitute.org)
- Scientists have characterized a sample of "mystery" fungus collected in North Carolina and found its home in the fungal tree of life. (lanl.gov)
- In 2012, the first cases of a fungal disease - dubbed "ash dieback" - that was wiping out ash trees across Europe were detected in the UK, probably after the fungus hitched a ride here on infected imported plants. (thenakedscientists.com)
- Regarding the infecting fungus undergo microevolution and dna. (daleko.space)
- A synopsis of these proposals, with comments by the Secretary (T.W. May) and Deputy Secretary (S.A. Redhead) of the Fungal Nomenclature Bureau, was published in IMA Fungus on 23 May 2018 (May and Redhead 2018 ). (biomedcentral.com)
Genomics1
- Do plant genomics and terrorism cases were based on factors: a mood of amplified by size in humans be dna fingerprinting and its application in paternity? (daleko.space)
Microorganisms1
- The research team, co-led by investigators at the University of California-San Diego School of Medicine and the Weizmann Institute of Science in Rehovot, Israel, noted that little is known about the role of fungal microorganisms, or mycobiota, a part of the human microbiome, in human cancers. (upi.com)
Taxonomic1
- The fungal sample was interesting partly as it represented a major component of the observed fungal population in a pine forest and it responded positively to elevated CO 2 and nitrogen amendment treatments that mimic future environmental conditions--yet the sample's exact placement in the taxonomic order was unknown. (lanl.gov)
Biomass6
- Fungal biomass is also a valuable product for food and textile industries, and most recently as a potential material in building construction (Table 1 ). (biomedcentral.com)
- Fungal and bacterial biomass and the F/BF/B ratio were higher in grass than in grass-clover. (louisbolk.nl)
- Partial N balance was more positive at a higher N application rate and showed an inverse relationship with fungal biomass and F/BF/B ratio. (louisbolk.nl)
- We conclude that the fungal/bacterial biomass ratio quickly responded to changes in management. (louisbolk.nl)
- Grass-clover had a smaller fungal biomass and higher N leaching than grass. (louisbolk.nl)
- In general, a higher fungal biomass indicated a lower nitrogen leaching and a more negative partial N balance (or smaller N surplus), but more observations are needed to confirm the relationship between F/BF/B ratio and sustainability. (louisbolk.nl)
Yeasts1
- To date, the presumptive infectious etiologies include classically-reported fungal dermatophytes, non-dermatophyte molds, and yeasts. (japmaonline.org)
Growth3
- Prompt reduction of immunosuppression and initiation of No fungal growth was detected in a cerebrospinal antifungal therapy was required to successfully control fluid sample. (cdc.gov)
- Moving at a rate of around 6 kilometers per year from the equator towards both poles, fungal growth and pests pose a serious risk to previously safe land. (brinknews.com)
- Experiments designed to test fungal growth in microgravity are set to launch in late 2019. (eurekalert.org)
Cells2
- Those cells are designed to become porous to extracellular DNA when treated with calcium chloride and provided with a heat shock of 42°C. At a minimum I'd think they'd include a thermometer so you can control the heat shock process. (hackaday.com)
- Ionizing radiation kills cells by damaging their DNA and other essential cellular infrastructure. (eurekalert.org)
Plant4
- We used QIAGEN DNeasy 96 Plant Kit to extract the DNA (following the protocol for frozen plant tissue). (bestpfe.com)
- Member of the editorial boards for journals Fungal Ecology and Plant Pathology . (aber.ac.uk)
- To get a new estimate, a lot of work needs to be done on plant leaves, decomposing wood, sediments, and surface waters - all important reservoirs for fungal richness. (aaas.org)
- As an alternative to manual microscopic analysis of dust components, we developed a pipeline that utilizes the airborne plant environmental DNA (eDNA) in settled dust to estimate geographic origin. (mit.edu)
Biological2
- Karczmarczyk M, Bartoszcze M. DNA microarrays - new tool in the identification of biological agents. (aaem.pl)
- Biological identifications through DNA barcodes. (unimilitar.edu.co)
Biotechnology2
- Ultimately, these advances will not only expand the fungal biotechnology portfolio but will also significantly contribute to a sustainable bio-economy. (biomedcentral.com)
- Programme Support on Molecular Parasitology" to work on the project entitled "Characterization of DNA replication and non-replication function of two putative homologues of Plasmodium falciparum Origin Recognition Complex" Department of Biotechnology (DBT) (2011-2016). (jnu.ac.in)
Purification2
- it does not describe the procedure for purification of fungal DNA. (cdc.gov)
- BAC DNA Purification for Microinjection · Chromosome Counting · DNA Purification for Electroporation · DNA Purification for. (logansquareauditorium.com)