Regulation of body length and male tail ray pattern formation of Caenorhabditis elegans by a member of TGF-beta family. (1/28861)
We have identified a new member of the TGF-beta superfamily, CET-1, from Caenorhabditis elegans, which is expressed in the ventral nerve cord and other neurons. cet-1 null mutants have shortened bodies and male tail abnormal phenotype resembling sma mutants, suggesting cet-1, sma-2, sma-3 and sma-4 share a common pathway. Overexpression experiments demonstrated that cet-1 function requires wild-type sma genes. Interestingly, CET-1 appears to affect body length in a dose-dependent manner. Heterozygotes for cet-1 displayed body lengths ranging between null mutant and wild type, and overexpression of CET-1 in wild-type worms elongated body length close to lon mutants. In male sensory ray patterning, lack of cet-1 function results in ray fusions. Epistasis analysis revealed that mab-21 lies downstream and is negatively regulated by the cet-1/sma pathway in the male tail. Our results show that cet-1 controls diverse biological processes during C. elegans development probably through different target genes. (+info)Molecular cloning and epitope analysis of the peanut allergen Ara h 3. (2/28861)
Peanut allergy is a significant IgE-mediated health problem because of the increased prevalence, potential severity, and chronicity of the reaction. Following our characterization of the two peanut allergens Ara h 1 and Ara h 2, we have isolated a cDNA clone encoding a third peanut allergen, Ara h 3. The deduced amino acid sequence of Ara h 3 shows homology to 11S seed-storage proteins. The recombinant form of this protein was expressed in a bacterial system and was recognized by serum IgE from approximately 45% of our peanut-allergic patient population. Serum IgE from these patients and overlapping, synthetic peptides were used to map the linear, IgE-binding epitopes of Ara h 3. Four epitopes, between 10 and 15 amino acids in length, were found within the primary sequence, with no obvious sequence motif shared by the peptides. One epitope is recognized by all Ara h 3-allergic patients. Mutational analysis of the epitopes revealed that single amino acid changes within these peptides could lead to a reduction or loss of IgE binding. By determining which amino acids are critical for IgE binding, it might be possible to alter the Ara h 3 cDNA to encode a protein with a reduced IgE-binding capacity. These results will enable the design of improved diagnostic and therapeutic approaches for food-hypersensitivity reactions. (+info)ETO-2, a new member of the ETO-family of nuclear proteins. (3/28861)
The t(8;21) is associated with 12-15% of acute myelogenous leukemias of the M2 subtype. The translocation results in the fusion of two genes, AML1 (CBFA2) on chromosome 21 and ETO (MTG8) on chromosome 8. AML1 encodes a DNA binding factor; the ETO protein product is less well characterized, but is thought to be a transcription factor. Here we describe the isolation and characterization of ETO-2, a murine cDNA that encodes a new member of the ETO family of proteins. ETO-2 is 75% identical to murine ETO and shares very high sequence identities over four regions of the protein with ETO (domain I-III and zinc-finger). Northern analysis identifies ETO-2 transcripts in many of the murine tissues analysed and in the developing mouse embryo. ETO-2 is also expressed in myeloid and erythroid cell lines. We confirmed the nuclear localization of ETO-2 and demonstrated that domain III and the zinc-finger region are not required for nuclear localization. We further showed that a region within ETO, containing domain II, mediates dimerization among family members. This region is conserved in the oncoprotein AML-1/ETO. The recent identification of another ETO-like protein, myeloid translocation gene-related protein 1, together with the data presented here, demonstrates that at least three ETO proteins exist with the potential to form dimers in the cell nucleus. (+info)Control of growth and differentiation by Drosophila RasGAP, a homolog of p120 Ras-GTPase-activating protein. (4/28861)
Mammalian Ras GTPase-activating protein (GAP), p120 Ras-GAP, has been implicated as both a downregulator and effector of Ras proteins, but its precise role in Ras-mediated signal transduction pathways is unclear. To begin a genetic analysis of the role of p120 Ras-GAP we identified a homolog from the fruit fly Drosophila melanogaster through its ability to complement the sterility of a Schizosaccharomyces pombe (fission yeast) gap1 mutant strain. Like its mammalian homolog, Drosophila RasGAP stimulated the intrinsic GTPase activity of normal mammalian H-Ras but not that of the oncogenic Val12 mutant. RasGAP was tyrosine phosphorylated in embryos and its Src homology 2 (SH2) domains could bind in vitro to a small number of tyrosine-phosphorylated proteins expressed at various developmental stages. Ectopic expression of RasGAP in the wing imaginal disc reduced the size of the adult wing by up to 45% and suppressed ectopic wing vein formation caused by expression of activated forms of Breathless and Heartless, two Drosophila receptor tyrosine kinases of the fibroblast growth factor receptor family. The in vivo effects of RasGAP overexpression required intact SH2 domains, indicating that intracellular localization of RasGAP through SH2-phosphotyrosine interactions is important for its activity. These results show that RasGAP can function as an inhibitor of signaling pathways mediated by Ras and receptor tyrosine kinases in vivo. Genetic interactions, however, suggested a Ras-independent role for RasGAP in the regulation of growth. The system described here should enable genetic screens to be performed to identify regulators and effectors of p120 Ras-GAP. (+info)Mammalian staufen is a double-stranded-RNA- and tubulin-binding protein which localizes to the rough endoplasmic reticulum. (5/28861)
Staufen (Stau) is a double-stranded RNA (dsRNA)-binding protein involved in mRNA transport and localization in Drosophila. To understand the molecular mechanisms of mRNA transport in mammals, we cloned human (hStau) and mouse (mStau) staufen cDNAs. In humans, four transcripts arise by differential splicing of the Stau gene and code for two proteins with different N-terminal extremities. In vitro, hStau and mStau bind dsRNA via each of two full-length dsRNA-binding domains and tubulin via a region similar to the microtubule-binding domain of MAP-1B, suggesting that Stau cross-links cytoskeletal and RNA components. Immunofluorescent double labeling of transfected mammalian cells revealed that Stau is localized to the rough endoplasmic reticulum (RER), implicating this RNA-binding protein in mRNA targeting to the RER, perhaps via a multistep process involving microtubules. These results are the first demonstration of the association of an RNA-binding protein in addition to ribosomal proteins, with the RER, implicating this class of proteins in the transport of RNA to its site of translation. (+info)Identification and characterization of the human orthologue of yeast Pex14p. (6/28861)
Pex14p is a central component of the peroxisomal protein import machinery, which has been suggested to provide the point of convergence for PTS1- and PTS2-dependent protein import in yeast cells. Here we describe the identification of a human peroxisome-associated protein (HsPex14p) which shows significant similarity to the yeast Pex14p. HsPex14p is a carbonate-resistant peroxisomal membrane protein with its C terminus exposed to the cytosol. The N terminus of the protein is not accessible to exogenously added antibodies or protease and thus might protrude into the peroxisomal lumen. HsPex14p overexpression leads to the decoration of tubular structures and mislocalization of peroxisomal catalase to the cytosol. HsPex14p binds the cytosolic receptor for the peroxisomal targeting signal 1 (PTS1), a result consistent with a function as a membrane receptor in peroxisomal protein import. Homo-oligomerization of HsPex14p or interaction of the protein with the PTS2-receptor or HsPex13p was not observed. This distinguishes the human Pex14p from its counterpart in yeast cells and thus supports recent data suggesting that not all aspects of peroxisomal protein import are conserved between yeasts and humans. The role of HsPex14p in mammalian peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible for an unrecognized complementation group of human peroxisome biogenesis disorders. (+info)Down-regulation of RpS21, a putative translation initiation factor interacting with P40, produces viable minute imagos and larval lethality with overgrown hematopoietic organs and imaginal discs. (7/28861)
Down-regulation of the Drosophila ribosomal protein S21 gene (rpS21) causes a dominant weak Minute phenotype and recessively produces massive hyperplasia of the hematopoietic organs and moderate overgrowth of the imaginal discs during larval development. Here, we show that the S21 protein (RpS21) is bound to native 40S ribosomal subunits in a salt-labile association and is absent from polysomes, indicating that it acts as a translation initiation factor rather than as a core ribosomal protein. RpS21 can interact strongly with P40, a ribosomal peripheral protein encoded by the stubarista (sta) gene. Genetic studies reveal that P40 underexpression drastically enhances imaginal disc overgrowth in rpS21-deficient larvae, whereas viable combinations between rpS21 and sta affect the morphology of bristles, antennae, and aristae. These data demonstrate a strong interaction between components of the translation machinery and showed that their underexpression impairs the control of cell proliferation in both hematopoietic organs and imaginal discs. (+info)DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines. (8/28861)
The Src homology 3 (SH3) motif is found in numerous signal transduction proteins involved in cellular growth and differentiation. We have purified and cloned a novel protein, DEF-1 (differentiation-enhancing factor), from bovine brain by using a Src SH3 affinity column. Ectopic expression of DEF-1 in fibroblasts resulted in the differentiation of a significant fraction of the culture into adipocytes. This phenotype appears to be related to the induction of the transcription factor peroxisome proliferator-activated receptor gamma (PPARgamma), since DEF-1 NIH 3T3 cells demonstrated augmented levels of PPARgamma mRNA and, when treated with activating PPARgamma ligands, efficient induction of differentiation. Further evidence for a role for DEF-1 in adipogenesis was provided by heightened expression of DEF-1 mRNA in adipose tissue isolated from obese and diabetes mice compared to that in tissue isolated from wild-type mice. However, DEF-1 mRNA was detected in multiple tissues, suggesting that the signal transduction pathway(s) in which DEF-1 is involved is not limited to adipogenesis. These results suggest that DEF-1 is an important component of a signal transduction process that is involved in the differentiation of fibroblasts and possibly of other types of cells. (+info)
Complementary DNA
In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA ( ... Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library. When scientists ... "Cloning of Complementary DNA Encoding a Functional Human Interleukin-8 Receptor." Science (American Association for the ... "Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project." Science (American Association for the ...
Glossary of genetics (0-L)
cDNA See complementary DNA. cell-free DNA (cfDNA) Any DNA molecule that exists outside of a cell or nucleus, freely floating in ... copy DNA (cDNA) See complementary DNA. copy error A mutation resulting from a mistake made during DNA replication. copy-number ... Complementarity is fundamental to the mechanisms governing DNA replication, transcription, and DNA repair. complementary DNA ( ... either DNA-DNA, RNA-RNA, or a DNA-RNA hybrid) which are held together by hydrogen bonds between the complementary nucleobases ...
Glossary of genetics
cDNA See complementary DNA. cell-free DNA (cfDNA) Any DNA molecule that exists outside of a cell or nucleus, freely floating in ... copy DNA (cDNA) See complementary DNA. copy error A mutation resulting from a mistake made during DNA replication. copy-number ... Complementarity is fundamental to the mechanisms governing DNA replication, transcription, and DNA repair. complementary DNA ( ... either DNA-DNA, RNA-RNA, or a DNA-RNA hybrid) which are held together by hydrogen bonds between the complementary nucleobases ...
DNA
In DNA, fraying occurs when non-complementary regions exist at the end of an otherwise complementary double-strand of DNA. ... DNA exists in many possible conformations that include A-DNA, B-DNA, and Z-DNA forms, although only B-DNA and Z-DNA have been ... In DNA replication, DNA-dependent DNA polymerases make copies of DNA polynucleotide chains. To preserve biological information ... DNA at Curlie DNA binding site prediction on protein DNA the Double Helix Game From the official Nobel Prize web site DNA under ...
Variant of uncertain significance
Nucleotide sequences derived from complementary DNA". primary. The Journal of Biological Chemistry. 252 (14): 5040-53. doi: ... That is, a difference can be seen in the DNA sequence, but the differences have no effect on the growth or health of the person ... A recent study of the genes ATM, BRCA1, BRCA2, CDH1, CHEK2, PALB2 and TP53 found 15,311 DNA sequence variants in only 102 ... January 2017). "Targeted DNA sequencing and in situ mutation analysis using mobile phone microscopy". primary. Nature ...
Obsolete models of DNA structure
Crick, F. & Watson, J. D. (1954). "The Complementary Structure of Deoxyribonucleic Acid" (PDF). Proceedings of the Royal ... and Z-form DNA. Even once the DNA duplex structure was solved, it was initially an open question whether additional DNA ... The DNA double helix was discovered in 1953 (with further details in 1954) based on X-ray diffraction images of DNA as well as ... Under torsional stress, a Z-DNA structure can form with opposite twist to B-form DNA, but this is rare within the cellular ...
Chargaff's rules
Rudner, R; Karkas, JD; Chargaff, E (1968). "Separation of B. Subtilis DNA into complementary strands. 3. Direct analysis". ... During replication the DNA strands separate. In single stranded DNA, cytosine spontaneously slowly deaminates to adenosine (a C ... The second rule holds that both Α%≈ Τ% and G% ≈ C% are valid for each of the two DNA strands. This describes only a global ... Chargaff's rules state that in the DNA of any species and any organism, the amount of guanine should be equal to the amount of ...
Israel Hanukoglu
Curriculum Vitae Hanukoglu I, Tanese N, Fuchs E (Feb 1983). "Complementary DNA sequence of a human cytoplasmic actin. ...
Beta-actin
Hanukoglu I, Tanese N, Fuchs E (Feb 1983). "Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of ...
PSAT1
Cloning and sequence analysis of the complementary DNA". Biochemistry. 26 (13): 3975-82. doi:10.1021/bi00387a035. PMID 3651428 ...
Thromboxane-A synthase
Wang LH, Ohashi K, Wu KK (1991). "Isolation of partial complementary DNA encoding human thromboxane synthase". Biochem. Biophys ... and interaction of transcriptional regulatory factor with its DNA element in the 5' flanking region". Biochim. Biophys. Acta. ...
IL1RL1
"Nucleotide sequence of a complementary DNA for human ST2". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression ... "Nucleotide sequence of a complementary DNA for human ST2". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression ... Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 ...
Estrogen receptor alpha
"Sequence and expression of human estrogen receptor complementary DNA". Science. 231 (4742): 1150-1154. Bibcode:1986Sci... ... Teo AK, Oh HK, Ali RB, Li BF (October 2001). "The modified human DNA repair enzyme O(6)-methylguanine-DNA methyltransferase is ... "Sequence and expression of human estrogen receptor complementary DNA". Science. 231 (4742): 1150-1154. Bibcode:1986Sci... ... Thénot S, Bonnet S, Boulahtouf A, Margeat E, Royer CA, Borgna JL, Cavaillès V (December 1999). "Effect of ligand and DNA ...
Poliovirus
Racaniello VR, Baltimore D (November 1981). "Cloned poliovirus complementary DNA is infectious in mammalian cells". Science. ... Scientists first converted poliovirus's published RNA sequence, 7741 bases long, into a DNA sequence, as DNA was easier to ... Enzymes were used to convert the DNA back into RNA, its natural state. Other enzymes were then used to translate the RNA into a ... DNA encoding the RNA genome of poliovirus was introduced into cultured mammalian cells and infectious poliovirus was produced. ...
Calcyphosin
"Cloning and sequence analysis of human calcyphosine complementary DNA". Biochim Biophys Acta. 1352 (3): 249-52. doi:10.1016/ ... 2004). "The DNA sequence and biology of human chromosome 19". Nature. 428 (6982): 529-35. Bibcode:2004Natur.428..529G. doi: ...
Vincent Racaniello
Production of complementary DNA representing RNA viral sequences by recombinant DNA methods and uses therefor, retrieved 2014- ... Racaniello, Vincent R.; David Baltimore (1981-11-20). "Cloned Poliovirus Complementary DNA is Infectious in Mammalian Cells". ... Racaniello used recombinant DNA technology to clone and sequence the genome of the small RNA animal virus poliovirus. Using ...
TPSAB1
Miller JS, Moxley G, Schwartz LB (Oct 1990). "Cloning and characterization of a second complementary DNA for human tryptase". J ... Miller JS, Westin EH, Schwartz LB (1989). "Cloning and characterization of complementary DNA for human tryptase". J. Clin. ...
CDNA library
This results in the creation of RNA-DNA hybrids where a single strand of complementary DNA is bound to a strand of mRNA. To ... When studying eukaryotic DNA, expression libraries are constructed using complementary DNA (cDNA) to help ensure the insert is ... A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which ... DNA polymerase I is then added, the cleaved RNA acts as a primer the DNA polymerase I can identify and initiate replacement of ...
Nucleic acid structure
DNA is double-stranded containing both a sense strand and an antisense strand. Therefore, the complementary sequence will be to ... B-DNA is the most common form of DNA in vivo and is a more narrow, elongated helix than A-DNA. Its wide major groove makes it ... A-DNA, is a form of the DNA duplex observed under dehydrating conditions. It is shorter and wider than B-DNA. RNA adopts this ... Biology portal Biomolecular structure Crosslinking of DNA DNA nanotechnology DNA supercoil Gene structure Non-helical models of ...
Transcriptomics technologies
June 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. ... RNA is first copied as complementary DNA (cDNA) by a reverse transcriptase enzyme before the resultant cDNA is sequenced. ... Libraries of silkmoth mRNA transcripts were collected and converted to complementary DNA (cDNA) for storage using reverse ... Shalon D, Smith SJ, Brown PO (July 1996). "A DNA microarray system for analyzing complex DNA samples using two-color ...
Serial analysis of gene expression
June 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. ... In 1979 teams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of ... The general goal of the technique is similar to the DNA microarray. However, SAGE sampling is based on sequencing mRNA output, ... The cDNA concatemers can then be isolated and sequenced using modern high-throughput DNA sequencers, and these sequences can be ...
Expressed sequence tag
Because these clones consist of DNA that is complementary to mRNA, the ESTs represent portions of expressed genes. They may be ... Gene expression Complementary DNA (cDNA) transcriptomics IMAGE cDNA clones Whole genome sequencing (WGS) ESTs Factsheet. ... Jun 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. ... In 1979, teams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of ...
CALM2
"Molecular analysis of human and rat calmodulin complementary DNA clones. Evidence for additional active genes in these species ...
DNA sequencing
Complementary DNA can then be processed the same way as genomic DNA. According to the sequencing technology to be used, the ... large DNA fragments into shorter DNA fragments. The fragmented DNA may then be cloned into a DNA vector and amplified in a ... DNA sequencing may be used along with DNA profiling methods for forensic identification and paternity testing. DNA testing has ... One key issue is the ownership of an individual's DNA and the data produced when that DNA is sequenced. Regarding the DNA ...
CALM3
SenGupta B, Friedberg F, Detera-Wadleigh SD (December 1987). "Molecular analysis of human and rat calmodulin complementary DNA ... In the lab experiment with rats, lambda rCB1 or hCE1 underwent DNA isolation as both of the genes included the CALM-3 gene, and ... The CALM-3 gene, along with the protein of calmodulin, has been included in different types of experiments such as DNA ...
Orange petunia
This transgenic complementary DNA enables production of orange pelargonidin pigment. One study of three commercially ... After analyzing a sample he discovered that the DNA of the flowers matched the modification described in the original 1987 ... Text taken from Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified ... "Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified orange Petunia". ...
TPSB2
Miller JS, Moxley G, Schwartz LB (September 1990). "Cloning and characterization of a second complementary DNA for human ...
Group II pyridoxal-dependent decarboxylases
"Characterization and expression of the complementary DNA encoding rat histidine decarboxylase". Proc. Natl. Acad. Sci. U.S.A. ...
SOD3
Hjalmarsson K, Marklund SL, Engström A, Edlund T (September 1987). "Isolation and sequence of complementary DNA encoding human ...
Gene signature
The second study identified a technique that is now widely known as the microarray which quantifies complementary DNA (cDNA) ... "Quantitative monitoring of gene expression patterns with a complementary DNA microarray". Science. 270 (5235): 467-70. doi: ... Pressing forward, the latter technique has revolutionized research in genetics and DNA chip technology as it is a widely ... Kurian KM, Watson CJ, Wyllie AH (February 1999). "DNA chip technology". The Journal of Pathology. 187 (3): 267-71. doi:10.1002 ...
Phage display
... and in searching for protein-DNA interactions using specially-constructed DNA libraries with randomised segments. Recently, ... cDNA has also been analyzed using pIII via a two complementary leucine zippers system, Direct Interaction Rescue or by adding ... In the case of M13 filamentous phage display, the DNA encoding the protein or peptide of interest is ligated into the pIII or ... By immobilizing a relevant DNA or protein target(s) to the surface of a microtiter plate well, a phage that displays a protein ...
Proto-oncogene tyrosine-protein kinase Src
Nada S, Okada M, MacAuley A, Cooper JA, Nakagawa H (May 1991). "Cloning of a complementary DNA for a protein-tyrosine kinase ...
Prokaryotic DNA replication
This is accomplished by distinguishing Watson-Crick base pairs through the use of an active site pocket that is complementary ... of either parent DNA or newly formed DNA and thereafter the ligating activity ligates that broken DNA strand and so the two DNA ... DNA polymerase III holoenzyme is loaded into the DNA and replication begins. The catalytic mechanism of DNA polymerase III ... The rate of DNA replication in a living cell was first measured as the rate of phage T4 DNA elongation in phage-infected E. ...
Antiparallel (biochemistry)
The importance of an antiparallel DNA double helix structure is because of its hydrogen bonding between the complementary ... "Re: Why are the two strands of a DNA molecule antiparallel?". www.madsci.org. Retrieved 2017-04-06. "why is DNA antiparallel? ... The antiparallel structure of DNA is important in DNA replication because it replicates the leading strand one way and the ... An example is the two complementary strands of a DNA double helix, which run in opposite directions alongside each other. ...
BMF (gene)
2001). "Characterization of long cDNA clones from human adult spleen". DNA Res. 7 (6): 357-66. doi:10.1093/dnares/7.6.357. PMID ... 2005). "Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function ...
Fragmentation (cell biology)
... purified DNA ligase is used to covalently join the ends of a restriction fragment and vector DNA that have complementary ends. ... 2. DNA ligase During normal DNA replication, DNA ligase catalyzes end-to-end joining (ligation) of short fragments of DNA, ... Recombinant DNA ↓ Replication of recombinant DNA within host cell ↓ Isolation, sequencing, and manipulation of purified DNA ... DNA ligase can ligate complementary sticky and blunt ends, but blunt-end ligation is inefficient and requires a higher ...
Cypriot Greek
DNA). Locally produced television shows, usually comedies or soap operas, make use of the dialect, for example with Vourate ... velar stops and fricatives are in complementary distribution with palatals and postalveolars before front vowels /e i/; that is ...
NOL1
"Increased growth of NIH/3T3 cells by transfection with human p120 complementary DNA and inhibition by a p120 antisense ...
RK2 plasmid
In addition, RK2 contains a set of potentially lethal (to the cell) genes, called kil genes, and a set of complementary ... selfish DNA molecules with a complicated regulatory circuit" RK2 was first isolated in connection with an outbreak of ...
Tetratricopeptide repeat protein 39B
The genomic DNA is 136,517 bases long, consists of 39 introns and 20 exons, and is on the minus strand. The mRNA has a length ... another create a right-handed helical structure with an amphipathic channel which could possibly accommodate the complementary ... Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, ...
Islam in India
Sufism is a mystical dimension of Islam, often complementary with the legalistic path of the sharia had a profound impact on ... Sources like The Times of India and DNA reported Indian Shia population during that period between 40,000,000 to 50,000,000 of ...
Short interspersed nuclear element
CS1 Russian-language sources (ru), Molecular biology, Repetitive DNA sequences, Mobile genetic elements, Non-coding DNA, ... The microRNAs are transcribed as part of longer RNA strands of generally about 80 nucleotides which through complementary base- ... Moreover, non-coding RNAs like SINEs can bind or interact directly with the DNA duplex coding the gene and thus prevent its ... This enables the LINE mRNA to be reverse-transcribed into DNA and integrated into the genome based on the sequence-motifs ...
Iron overload
A pilot study of mitochondrial DNA and Y-DNA". Canadian Journal of Gastroenterology. 25 (6): 324-326. doi:10.1155/2011/463810. ... 2009 Jonas: Mosby's Dictionary of Complementary and Alternative Medicine. 2005. "Hemochromatosis". Archived from the original ... DNA/screening: the current standard of practice in diagnosis of hemochromatosis, places emphasis on genetic testing. Positive ...
Herbal medicine
Twenty-four products were tested by DNA barcoding as part of the investigation, with all but five containing DNA that did not ... Few studies are available on the safety of herbs for pregnant women, and one study found that use of complementary and ... In the United States, the National Center for Complementary and Integrative Health of the National Institutes of Health funds ... Egede LE, Ye X, Zheng D, Silverstein MD (February 2002). "The prevalence and pattern of complementary and alternative medicine ...
RNA-targeting small molecule drugs
Small molecules may present a better way to target RNA and subsequently DNA because they can be designed to be more "drug-like ... By this principle, if one can identify an RNA involved in disease then the sequence can be used to design a complementary ... In 2009, the Zimmerman group discovered a compound to target the trinucleotide repeat expanded RNA and DNA that cause DM1. ... Traditionally, RNA was thought to be a mediator between DNA sequence-encoded instructions and functional protein. However, ...
Cot filtration
Briefly, when sheared genomic DNA in solution is heated to near boiling temperature, the molecular forces holding complementary ... In CF, genomic DNA is heat-denatured and allowed to renature to a Cot value (Cot = DNA concentration x time x a factor based on ... Double-stranded, repetitive DNA is separated from single-stranded, low-copy DNA by hydroxyapatite chromatography or other means ... This allows DNA sequencing to concentrate on the parts of the genome that are most informative and interesting. ...
Infrared sensing in vampire bats
Deep sequencing of complementary DNA of these receptor channels shows that this is not true for closely related fruit bats ...
Haplogroup L2 (mtDNA)
Mitochondrial DNA Site Ian Logan's L2bcd. Mitochondrial DNA Site Mannis van Oven's PhyloTree.org - mtDNA subtree L Spread of ... The complementary population and L0a phylogeography analyses indicate no strong evidence of mtDNA gene flow between eastern and ... Genealogical DNA test Genetic genealogy Human mitochondrial genetics Population genetics Human mitochondrial DNA haplogroups ... González, A. M. et al 2006, Mitochondrial DNA Variation in Mauritania and Mali and their Genetic Relationship to Other Western ...
Enzyme inhibitor
In a complementary technique, peptide mass fingerprinting involves digestion of the native and modified protein with a protease ... This is a potent enzyme inhibitor, in this case preventing the RNA polymerase II enzyme from transcribing DNA. The algal toxin ... Complementary approaches that can provide new starting points for inhibitors include fragment-based lead discovery and ... Satz AL, Kuai L, Peng X (May 2021). "Selections and screenings of DNA-encoded chemical libraries against enzyme and cellular ...
Multiplex ligation-dependent probe amplification
Pairs of probes are hybridized to the sample DNA, with each probe pair designed to query for the presence of a particular DNA ... which is a region that contains the sequence complementary to the region of interest, so that the correct hybridization can ... The process consists of multiple steps: The sample DNA is denatured, resulting in single-stranded sample DNA. ... The signal strengths of the probes are compared with those obtained from a reference DNA sample known to have two copies of the ...
Edward DeLong
... where he surveyed communities of picoplankton via DNA sequencing. With Pace and his group at Indiana University, DeLong ... the cells based on the binding of fluorescent probes to individual cells through use of oligonucleotides that are complementary ...
Fluorescence in situ hybridization
In biology, a probe is a single strand of DNA or RNA that is complementary to a nucleotide sequence of interest. RNA probes can ... Repetitive DNA sequences must be blocked by adding short fragments of DNA to the sample. The probe is then applied to the ... so that long enough strands of DNA or RNA (often 10-25 nucleotides) which are complementary to a given target sequence are ... Preparing DNA probes for one species and performing FISH with this probe allows one to visualize the distribution of this ...
Blakeslea trispora
As these two different sex types produce TSA, they sense sexually complementary cells and form gametangia. Eventually, those ... However, dysregulation of ROS can lead to DNA damaging, inactivation of enzymes and proteins, disruption of membranes. This ... TSA produced from carotene stimulates both sexually complementary cells to make contact with each other. TSA is considered an ...
Coding theory approaches to nucleic acid design
... in which one DNA strand is hybridized to its complementary strand through a series of hydrogen bonds. For the purpose of this ... DNA code construction refers to the application of coding theory to the design of nucleic acid systems for the field of DNA- ... DNA computing involves allowing synthetic oligonucleotide strands to hybridize in such a way as to perform computation. DNA ... Most of the focus on DNA coding has been on constructing large sets of DNA codewords with prescribed minimum distance ...
Brain
Doya, K (2000). "Complementary roles of basal ganglia and cerebellum in learning and motor control". Current Opinion in ... which allows variations in brain structure to be correlated with variations in DNA properties and neuroimaging. Animal brains ...
Asymmetric PCR
... can be used to form single stranded DNA from double stranded DNA, which is then used for DNA sequencing in the ... technique has applications in some types of sequencing and hybridization probing where having only one of the two complementary ... Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. The ... Wooddell, C I; Burgess, R R (1996). "Use of Asymmetric PCR to Generate Long Primers and Single-stranded DNA for Incorporating ...
Cloverleaf model of tRNA
The other end-the bottom often called the "DNA arm"-consists of a three base sequence that pairs with a complementary base ...
Cruciform DNA
As tRNA folds on itself in the presence of paired complementary bases, it causes the formation of branches and loops that are ... Cruciform DNA is a form of non-B DNA, or an alternative DNA structure. The formation of cruciform DNA requires the presence of ... B-DNA can form transient structures of cruciform DNA that act as recognition signals near origins of replication in the DNA of ... Cruciform DNA is found in both prokaryotes and eukaryotes and has a role in DNA transcription and DNA replication, double ...
Cycling probe technology
CPT uses a sequence specific chimeric probe which hybridizes to a complementary target DNA sequence and becomes a substrate for ... the cleaved fragments dissociate from the target DNA. Once dissociated, the target DNA is free to hybridize with a new probe, ... However, unlike PCR, CPT does not generate multiple copies of the target DNA itself, and the amplification of the signal is ... Cycling probe technology makes use of a chimeric nucleic acid probe to detect the presence of a particular DNA sequence. The ...
equipmentshare website - Complementary DNA (cDNA)
Heterochiral DNA with Complementary Strands in α-D and β-D Configuration: H-Bonded and Silver Mediated Base Pairs with Impact ... Heterochiral DNA with Complementary Strands in α-D and β-D Configuration: H-Bonded and Silver Mediated Base Pairs with Impact ... Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an ... Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an ...
OneClass: What step in DNA replication precedes the pairing of complementary bases? A. polymerization...
... polymerization of DNA. B. formation of the histone co ... What step in DNA replication precedes the pairing of ... of the two DNA molecules, one is made up totally of parental DNA, while the other is entirely newly synthesized DNA. ... all the DNA strands in the two DNA molecules will have both parental and newly synthesized DNA. ... DNA replication of a single DNA molecule is referred to as semiconservative because ...
Creator: Crick, Francis, 1916-2004 / Genre: Articles - Francis Crick - Profiles in Science Search Results
Fractionation of the complementary strands of coliphage T4 DNA based on the asymmetric distribution of the poly U and poly U,G...
Structure, function, and regional distribution of the organic cation transporter OCT3 in the kidney
James Watson Quotes - 33 Science Quotes - Dictionary of Science Quotations and Scientist Quotes
Science quotes on: , Accomplishment (101) , Author (174) , Companion (21) , Complementary (15) , DNA (80) , Formation (100) , ... of DNA self replication is accomplished without recourse to specific protein synthesis and that each of our complementary DNA ... Science quotes on: , Acid (83) , Amino Acid (12) , Biochemistry (50) , Central (81) , Class (168) , Direct (228) , DNA (80) , ... Science quotes on: , Belief (605) , DNA (80) , Stress (22) , Structure (364) If, for example, I had some idea, which, as it ...
Cylinder collection by LE KLINT's own design team - Scandinaviandesign.com
Antiparallel structure of DNA strands (video) | Khan Academy
So this would be an anti... antiparallel structure of DNA. So these two strands, theyre complementary. Theyre defined by each ... DNA base pairs always match up correctly. What DOES happen, causing mutations, is that the order of base pairs in the DNA ... Voiceover] In the video on the molecular structure of DNA we saw that DNA is typically made up of two strands where the ... So these are both ribose, we saw that in the molecular structure of DNA videos. When were talking about DNA were talking ...
International Journal of Computational Systems Engineering (IJCSysE) Inderscience Publishers - linking academia, business and...
Thogotoviruses/Bourbon Virus: Background, Pathophysiology, Epidemiology and Transmission
Tuberculosis Transmission in a Homeless Shelter Population --- New
York, 2000--2003
Determination of complete chromosomal haplotypes by bulk DNA sequencing | bioRxiv
is the complementary phase block of Bk.). Finally, maximizing Eq. (6) is equivalent to minimizing the energy of a 1D Ising ... or indirectly from short DNA fragments derived from long DNA molecules (~100kb) that are tagged with unique molecular barcodes ... Shendure, J. and Aiden, E. L. (2012). The expanding scope of DNA sequencing. Nature Publishing Group, 30(11):1084-1094. ... Determination of complete chromosomal haplotypes by bulk DNA sequencing. Richard W. Tourdot, View ORCID ProfileCheng-Zhong ...
PuRe Publications | Max Planck Institute for Molecular Genetics
JCM | Free Full-Text | Prevalence of Herpesvirus DNA in Corneal Transplant Recipients
The recipient corneas of 91 patients without a history of HK tested positive for HSV-1 DNA in 12 (13.2%) eyes, for VZV DNA in 3 ... eyes and VZV DNA in 1 (4.8%) eye. All patients with positive herpes DNA and no history of HK prior to PK received antiherpetic ... The recipient corneas of 21 patients with a preoperative history of HK tested positive for HSV-1 DNA in 13 (61.9%) ... Conclusions: We found herpesvirus DNA in 18.7% of recipient corneas without clinical suspicion or history of herpes keratitis. ...
https://www.wikidoc.org/index.php/Complementary DNA
DNA origami to scale-up molecular motors | EurekAlert!
This self-assembly was made possible by the binding of complementary DNA strands attached to each component. ... The DNA origami were formed from six DNA helices bundled together. Mixing the two components caused the microtubules to self- ... DNA origami to scale-up molecular motors. Hokkaido University. Journal. Nano Letters. Funder. Japan, New Energy and Industrial ... video: The microtubule network contracting in the presence of DNA origami, kinesins, and ATP. (Matsuda K. ,em,et al,/em,., ,em, ...
Polk-Pollock-Pogue DNA Project - Overview | FamilyTreeDNA
... and unlock the secrets of your ancestry and genealogy with our DNA kits for ancestry and the worlds most comprehensive DNA ... DNA can be tested in different ways which are quite distinct and complementary. These include STR marker testing, SNP testing ( ... Big Y-700), autosomal DNA (Family Finder) and mitochondrial DNA (mtDNA) testing. All help in determining lines of descent. ... The Polk-Pollock-Pogue (PPP) DNA Project is open to all persons claiming Polk-Pollock-Pogue, etc, ancestry through blood or ...
Label-free detection of real-time DNA amplification using a nanofluidic diffraction grating | Scientific Reports
We showed the first case reported to date for label-free detection of real-time DNA amplification, such as specific DNA ... Here we report a label-free detection of real-time DNA amplification using a nanofluidic diffraction grating. Our detection ... Since our developed system allows quantification of the initial concentration of amplified DNA molecules ranging from 1 fM to 1 ... Quantitative DNA amplification using fluorescence labeling has played an important role in the recent, rapid progress of basic ...
Filaggrin gene polymorphisms in Indian children with atopic dermatitis: A cross-sectional multicentre study - Indian Journal of...
VarClass, variant classifier; cDNA, complementary DNA; AA, amino acid; dbSNP ID, single nucleotide polymorphism database; ... Genomic DNA was extracted from peripheral blood (n = 75) using the genomic DNA extraction kit (Qiagen). DNA samples were ... VarClass, variant classifier; cDNA, complementary DNA; AA, amino acid; dbSNP ID, single nucleotide polymorphism database; ... Five children failed the DNA quality control (insufficient DNA sample), hence we analysed 75 children [Figure 1]. Among these ...
TRIC J R
... which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. The labeled DNA ... During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer, and ... The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and ... The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of ...
infoholix.net - Seiki - Complementary Alternative Medicine International Directory
Frontiers | Epithelial Bone Morphogenic Protein 2 and 4 Are Indispensable for Tooth Development
Guanine - New World Encyclopedia
In DNA, guanine and adenine form hydrogen bonds with their complementary pyrimidine derivatives, cytosine and thymine. In RNA, ... Thus, guanine, along with adenine and cytosine, is present in both DNA and RNA, whereas thymine is usually seen only in DNA and ... Guanine, a two-ring molecular structure, is one of the five defining components or nucleobases found in the nucleic acids DNA ... The ubiquitousness of guanine, which plays a central role in the the DNA of all living organisms and even in RNA viruses is ...
Faculty and Credentialed Providers | School of Medicine | University of Utah Health
Lim CS, Hunt CA (1997). Synthesis of DNA dumbbells: chemical vs. enzymatic ligation of self-complementary oligonucleotides. ... Lim, C.S., Matissek, K.M., and Okal, A. (2014). Targeting p53 and its DNA Binding Domain. U.S. Patent No. PCT/US2015/014851. ... Matissek KJ, Mossalam M, Okal A, Lim CS (2013). The DNA binding domain of p53 is sufficient to trigger a potent apoptotic ... Sequence-independent inhibition of RNA transcription by DNA dumbbells and other decoys. Nucleic Acids Res, 25(3), 575-81. ...
Biblio | College of Agricultural Sciences
Virus - Wikispecies
Nucleic acid (RNA or DNA): R or D *Capsid symmetry (helical, cubical or dual): H, C or B *Presence of a lipid envelope (naked ... Group V: Single-stranded RNA viruses whose genome is complementary in base sequence to the mRNA (-ssRNA) ... Group VII: Double-stranded DNA viruses that replicate through a single-stranded RNA intermediate (dsDNA-RT) ... Group VI: Single-stranded RNA viruses that replicate through a DNA intermediate (ssRNA-RT) ...
Flesh-eating flies map forest biodiversity | BioEd Online
DNA in insects guts reveals inventory of rare mammals. ... The DNA is "not gorgeous, but still usable", says Calvignac- ... "Really, the methods are very complementary," he says.. News. *Community Outreach. *Nature News - Archive *Gene edits to CRISPR ... Scientists in Germany have now shown that this DNA persists for long enough to be sequenced, providing a quick and cost- ... They can sample where we cant." However, he notes that DNA is more stably preserved in leeches, so it lasts longer. " ...
Structure of a nicked DNA-protein complex isolated from simian virus 40: Covalent attachment of the protein to DNA and nick...
The nick occurs on either of the two complementary strands; the specific nick sites on the two strands are staggered, but lie ... is covalently attached to the end of a DNA single strand. (Nicked DNA is double-stranded DNA that contains at least one single- ... A portion of the nicked circular DNA isolated from purified simian virus 40 contains a protein-DNA complex in which protein(s) ... Structure of a nicked DNA-protein complex isolated from simian virus 40: Covalent attachment of the protein to DNA and nick ...
Expanding the Allelic Heterogeneity of ANO10-Associated Autosomal Recessive Cerebellar Ataxia | Neurology Genetics
Complementary DNA Analysis. Reverse transcription PCR was performed on messenger RNA (mRNA) extracted from either patient- ... Effects on splicing were studied using reverse transcriptase PCR to analyze complementary DNA. Western blots were used to ... was used to synthesize complementary DNA (cDNA). ANO10 was amplified from cDNA between exons 6 and 8 (primers 5′- ... DNA was extracted using Qiagen gel extraction kit and submitted for Sanger sequencing. All kits were used according to the ...
CDNA2
- The Court specifically mentioned the ability to patent a type of DNA known as complementary DNA (cDNA). (medlineplus.gov)
- cDNA (i.e., complementary DNA, not circular DNA, not C- DNA ) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. (bvsalud.org)
Molecules9
- each of the two DNA molecules will consist of one parental strand and one newly synthesized strand. (oneclass.com)
- all the DNA strands in the two DNA molecules will have both parental and newly synthesized DNA. (oneclass.com)
- it results in two nonidentical DNA molecules. (oneclass.com)
- of the two DNA molecules, one is made up totally of parental DNA, while the other is entirely newly synthesized DNA. (oneclass.com)
- The typical size of DNA molecules in long-read or long-range sequencing (10-100kb) is suffcient for linking variants in regions of normal variant density (~1 per kb), but inadequate in regions of low variant density (1 per 10 kb), and unable to bridge large gaps (100kb) with no identifiable variants, including all centromeres. (biorxiv.org)
- Since our developed system allows quantification of the initial concentration of amplified DNA molecules ranging from 1 fM to 1 pM, we expect that it will offer a new strategy for developing fundamental techniques of medical applications. (nature.com)
- The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)
- The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
- As a result, there are now two double-stranded DNA molecules in the nucleus that contain the same information. (cdc.gov)
Strands3
- Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
- Cell-free DNA (cfDNA) is extracellular strands of DNA present in body fluids. (cdc.gov)
- The DNA strands unwind and separate. (cdc.gov)
Polymerase3
- A viral polymerase complex composed of polymerase basic subunit 1, polymerase basic subunit 2, and polymerase acidic subunit synthesizes negative-strand viral RNA, capped polyadenylated mRNAs, and complementary positive-strand RNA. (medscape.com)
- Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. (bvsalud.org)
- Association of DNA sequence variation in mitochondrial DNA polymerase with mitochondrial DNA synthesis and risk of oral cancer. (cdc.gov)
Molecular6
- American biochemist and geneticist who shared the 1962 Nobel Prize for Physiology or Medicine for the discovery of the molecular structure of DNA. (todayinsci.com)
- Quantitative DNA amplification using fluorescence labeling has played an important role in the recent, rapid progress of basic medical and molecular biological research. (nature.com)
- On June 13, 2013, in the case of the Association for Molecular Pathology v. Myriad Genetics, Inc., the Supreme Court of the United States ruled that human genes cannot be patented in the U.S. because DNA is a "product of nature. (medlineplus.gov)
- NCCN reports ctDNA testing studies have shown high specificity but compromised sensitivity for detecting molecular biomarkers that guide therapeutic interventions, therefore data supports complementary testing to reduce turnaround time and increase yield of targetable alteration detection. (cdc.gov)
- El ADNc (es decir, ADN complementario, no ADN circular [ADN-C]) se utiliza en diversos experimentos de clonación molecular y asimismo sirve como sonda de hibridación específica. (bvsalud.org)
- NIH is supporting studies at various institutions that use molecular diagnostics-looking at a person's DNA to diagnose disease-to differentiate fibromyalgia from other pain conditions. (medlineplus.gov)
Sequences2
- We showed the first case reported to date for label-free detection of real-time DNA amplification, such as specific DNA sequences from tubercle bacilli (TB) and human papillomavirus (HPV). (nature.com)
- The Supreme Court's ruling did allow that DNA manipulated in a lab is eligible to be patented because DNA sequences altered by humans are not found in nature. (medlineplus.gov)
Molecule5
- The bases are bonded to what part of the backbone of the DNA molecule? (oneclass.com)
- A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
- This synthetic DNA is produced from the molecule that serves as the instructions for making proteins (called messenger RNA). (medlineplus.gov)
- Deoxyribonucleic acid (DNA) is a complex molecule of many components. (cdc.gov)
- This video will describe how these four groups build upon each other to create the DNA molecule. (cdc.gov)
Primers4
- For WGS, complementary DNA was prepared using random primers from viral RNA extracted from SARS-CoV-2 real-time RT-PCR-positive specimens. (cdc.gov)
- In comparison to other miRNA real-time PCR systems that use either stem-loop or standard DNA primers, the LNA-enhanced primers offer significantly increased sensitivity, especially for AU-rich miRNAs. (qiagen.com)
- O DNA do bacilo foi extraído utilizando-se um kit comercial e amplificadas por RT qPCR, com uso de primers específicos para as regiões Specific Repetitive Element (RLEP) do DNA de M. leprae Resultados Não houve diferença significativa no valor do ΔCt em nenhuma das concentrações de bacilos, indicando que não foi possível fazer a discriminação entre células vivas e inviáveis. (bvsalud.org)
- The bacillus DNA was extracted using a commercial kit and amplified by RT qPCR using specific primers for the Specific Repetitive Element (RLEP) regions of the M. leprae Results There was no significant difference in the ΔCt value at any of the bacilli concentrations, indicating that discrimination between live and non-viable cells was not possible. (bvsalud.org)
Assays1
- The genotoxic effects in the lung fibroblast (V79) cell line were examined using two complementary assays: the comet assay and micronucleus (MN) test. (cdc.gov)
Strand5
- one DNA strand is replicated continuously, while the other must be replicated discontinuously. (oneclass.com)
- Transcriptome analysis by strand-specific sequencing of complementary DNA. (mpg.de)
- These subunits synthesize viral genomic negative-strand DNA, complementary positive-strand RNA, and capped polyadenylated mRNAs. (medscape.com)
- These oligonucleotides have substantially increased affinity for their complementary strand, when compared with traditional DNA or RNA oligonucleotides. (qiagen.com)
- Each strand makes a complementary strand by adding the appropriate nucleotides. (cdc.gov)
Therapies4
- Gradually, Dr. Reardon says she came to embrace the idea of integrative medicine: The "whole-person" approach to integrative medicine augments science with complementary and alternative medicine (CAM) therapies to treat a patient's collective mind, body, and spirit. (texmed.org)
- As the debate over CAM's place in medicine continues, Texas Medical Association policy recommends physicians stay on top of evidence-based studies of complementary and alternative therapies and routinely ask patients about their use of CAM products and therapies. (texmed.org)
- Generally, the term "complementary" refers to therapies patients use in conjunction with Western medicine, while "alternative" refers to therapies patients are using in place of conventional medicine. (texmed.org)
- During that decade, the federal government established what's now known as the National Center for Complementary and Integrative Health (NCCIH), which conducts research on CAM therapies. (texmed.org)
Mitochondrial4
- These include STR marker testing, SNP testing (Big Y-700), autosomal DNA (Family Finder) and mitochondrial DNA (mtDNA) testing. (familytreedna.com)
- Mitochondrial DNA polymorphisms and biogenesis genes in primary and metastatic uveal melanoma cell lines. (cdc.gov)
- Metabolic Reprogramming in Response to Alterations of Mitochondrial DNA and Mitochondrial Dysfunction in Gastric Adenocarcinoma. (cdc.gov)
- Genetic Alterations in Mitochondrial DNA Are Complementary to Nuclear DNA Mutations in Pheochromocytomas. (cdc.gov)
Replication1
- OneClass: What step in DNA replication precedes the pairing of complementary bases? (oneclass.com)
Amplification4
- Here we report a label-free detection of real-time DNA amplification using a nanofluidic diffraction grating. (nature.com)
- Our detection system observed intensity changes during DNA amplification of diffracted light derived from the passage of a laser beam through nanochannels embedded in a microchannel. (nature.com)
- For the monitoring real-time DNA amplification, C2CA-based method still needs fluorescent detection using oligonucleotide-tagged fluorophores 5 , leading to hybridization issue. (nature.com)
- Here we report label-free detection of real-time DNA amplification in a C2CA manner, which has less amplification, fluorescence-reagent volume and hybridization issues. (nature.com)
Detection3
- A signal-on electrochemical aptasensor for rapid detection of aflatoxin B1 based on competition with complementary DNA. (cdna-clone.com)
- During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). (cdc.gov)
- The findings underscore the complementary role of rapid DNA genotyping in the detection of possible TB transmission in homeless populations. (cdc.gov)
Sequence1
- A gene patent is the exclusive rights to a specific sequence of DNA (a gene) given by a government to the individual, organization, or corporation who claims to have first identified the gene. (medlineplus.gov)
Alternative medicine2
- A study in the December 2008 edition of the Southern Medical Journal titled " Complementary and Alternative Medicine Use Among Primary Care Patients in West Texas " looked at a sample of patients from nine clinics serving low-income populations. (texmed.org)
- Complementary alternative medicine */ @156 DME 1. (cdc.gov)
Differences2
Specific2
- Circulating tumor DNA (ctDNA) is a specific type of cfDNA that originates from a primary tumor, circulating tumor cells or metastasized cells. (cdc.gov)
- Current National Comprehensive Cancer Network (NCCN) 2022 guidelines specific to NSCLC (V5.2022) advise plasma cell-free/circulating tumor DNA testing should not be used in lieu of histologic tissue diagnosis, although it can be considered in specific clinical circumstances (i.e., if a patient is not suitable for invasive tissue sampling, if insufficient material following pathologic confirmation or incomplete assessment of all recommended biomarkers in the initial diagnostic setting). (cdc.gov)
Role1
- The ubiquitousness of guanine, which plays a central role in the the DNA of all living organisms and even in RNA viruses is evidence of the connectedness and unity of all life . (newworldencyclopedia.org)
Health3
- The 2012 edition of the National Health Interview Survey found 33 percent of U.S. adults and 12 percent of children use complementary health approaches. (texmed.org)
- See " Complementary Health . (texmed.org)
- Researchers at NIH's National Center for Complementary and Integrative Health (NCCIH) and National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) are investigating how to improve treatment, diagnosis, and pain management for fibromyalgia patients. (medlineplus.gov)
Viruses1
- the viruses did not contain DNA. (oneclass.com)
Genetic2
- As should be expected with any ancient family, different genetic branches have emerged from DNA testing. (familytreedna.com)
- As it turns out, two principle genetic branches of our family have emerged from Y-chromosome DNA testing, plus several other much smaller ones. (familytreedna.com)
Similar2
- This results in unprecedented sensitivity and specificity and makes LNA-enhanced oligos excellent tools for detecting and differentiating small or highly similar DNA or RNA targets in many research applications. (qiagen.com)
- RNA is similar in structure to DNA but is involved in different cellular functions. (cdc.gov)
Techniques2
- and DNA techniques to stimulate cytotoxic T-lymphocyte the fourth approach evaluated vaccination with a concentrated, responses. (cdc.gov)
- The two techniques are complementary in that supercritical fluid extraction (SFE) is promising for the removal of analytes from sample material (Hawthorne 1988) and immunoassay is very analyte-selective and sensitive (Vanderlaan et al. (cdc.gov)
Term1
- ctDNA is currently the most common form of liquid biopsy, a term used to refer to utilizing circulating cancer cells, DNA and other omic markers from blood or fluid sample. (cdc.gov)
Form3
- In DNA, guanine and adenine form hydrogen bonds with their complementary pyrimidine derivatives, cytosine and thymine. (newworldencyclopedia.org)
- The labeled DNA probe combines with amplicon to form stable RNA: DNA hybrids. (cdc.gov)
- Through transcription the information contained in a section of DNA is replicated to form a new piece of messenger RNA (mRNA). (cdc.gov)
Type1
- This study evaluated the prevalence and graft survival of herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) DNA in recipient corneas during PK. (mdpi.com)
Test1
- DNA: Test of Structure? (nih.gov)
Results1
- The Polk-Pollock-Pogue (PPP) DNA Project is open to all persons claiming Polk-Pollock-Pogue, etc, ancestry through blood or marriage who are interested in discovering or clarifying their common family genealogy through DNA testing and sharing of results. (familytreedna.com)
Data1
- A 2015 updated American College of Obstetrics and Gynecology (ACOG) Committee Opinion (Tier 2) recommended conventional screening methods as the most appropriate choice for first-line screening for most women in the general obstetric population since cell-free DNA screening has limitations in performance and limited data on cost-effectiveness in the low-risk obstetric population. (cdc.gov)
Structure3
- In The Double Helix: A Personal Account of the Discovery of the Structure of DNA (1968, 2001), Preface, xi. (todayinsci.com)
- Purported remark made at The Eagle pub (28 Feb 1953), near the Cavendish Laboratory in Cambridge, to celebrate the fact that they, Crick and Watson, had unravelled the structure of DNA. (todayinsci.com)
- Stated by James Watson in The Double Helix: A Personal Account of the Discovery of the Structure of DNA (1968, 1998), 197. (todayinsci.com)
Damage1
- DNA damage and MN induction were found after exposure to all tested materials with the strongest effect seen for CNF. (cdc.gov)
Present1
- Thus, guanine, along with adenine and cytosine, is present in both DNA and RNA, whereas thymine is usually seen only in DNA and uracil only in RNA. (newworldencyclopedia.org)
Complex1
- Before a cell divides and DNA is passed from one cell to another, a complex process occurs. (cdc.gov)
Main1
- When we designed the collection our main focus was on our DNA. (scandinaviandesign.com)
