Regulation of body length and male tail ray pattern formation of Caenorhabditis elegans by a member of TGF-beta family. (1/28861)

We have identified a new member of the TGF-beta superfamily, CET-1, from Caenorhabditis elegans, which is expressed in the ventral nerve cord and other neurons. cet-1 null mutants have shortened bodies and male tail abnormal phenotype resembling sma mutants, suggesting cet-1, sma-2, sma-3 and sma-4 share a common pathway. Overexpression experiments demonstrated that cet-1 function requires wild-type sma genes. Interestingly, CET-1 appears to affect body length in a dose-dependent manner. Heterozygotes for cet-1 displayed body lengths ranging between null mutant and wild type, and overexpression of CET-1 in wild-type worms elongated body length close to lon mutants. In male sensory ray patterning, lack of cet-1 function results in ray fusions. Epistasis analysis revealed that mab-21 lies downstream and is negatively regulated by the cet-1/sma pathway in the male tail. Our results show that cet-1 controls diverse biological processes during C. elegans development probably through different target genes.  (+info)

Molecular cloning and epitope analysis of the peanut allergen Ara h 3. (2/28861)

Peanut allergy is a significant IgE-mediated health problem because of the increased prevalence, potential severity, and chronicity of the reaction. Following our characterization of the two peanut allergens Ara h 1 and Ara h 2, we have isolated a cDNA clone encoding a third peanut allergen, Ara h 3. The deduced amino acid sequence of Ara h 3 shows homology to 11S seed-storage proteins. The recombinant form of this protein was expressed in a bacterial system and was recognized by serum IgE from approximately 45% of our peanut-allergic patient population. Serum IgE from these patients and overlapping, synthetic peptides were used to map the linear, IgE-binding epitopes of Ara h 3. Four epitopes, between 10 and 15 amino acids in length, were found within the primary sequence, with no obvious sequence motif shared by the peptides. One epitope is recognized by all Ara h 3-allergic patients. Mutational analysis of the epitopes revealed that single amino acid changes within these peptides could lead to a reduction or loss of IgE binding. By determining which amino acids are critical for IgE binding, it might be possible to alter the Ara h 3 cDNA to encode a protein with a reduced IgE-binding capacity. These results will enable the design of improved diagnostic and therapeutic approaches for food-hypersensitivity reactions.  (+info)

ETO-2, a new member of the ETO-family of nuclear proteins. (3/28861)

The t(8;21) is associated with 12-15% of acute myelogenous leukemias of the M2 subtype. The translocation results in the fusion of two genes, AML1 (CBFA2) on chromosome 21 and ETO (MTG8) on chromosome 8. AML1 encodes a DNA binding factor; the ETO protein product is less well characterized, but is thought to be a transcription factor. Here we describe the isolation and characterization of ETO-2, a murine cDNA that encodes a new member of the ETO family of proteins. ETO-2 is 75% identical to murine ETO and shares very high sequence identities over four regions of the protein with ETO (domain I-III and zinc-finger). Northern analysis identifies ETO-2 transcripts in many of the murine tissues analysed and in the developing mouse embryo. ETO-2 is also expressed in myeloid and erythroid cell lines. We confirmed the nuclear localization of ETO-2 and demonstrated that domain III and the zinc-finger region are not required for nuclear localization. We further showed that a region within ETO, containing domain II, mediates dimerization among family members. This region is conserved in the oncoprotein AML-1/ETO. The recent identification of another ETO-like protein, myeloid translocation gene-related protein 1, together with the data presented here, demonstrates that at least three ETO proteins exist with the potential to form dimers in the cell nucleus.  (+info)

Control of growth and differentiation by Drosophila RasGAP, a homolog of p120 Ras-GTPase-activating protein. (4/28861)

Mammalian Ras GTPase-activating protein (GAP), p120 Ras-GAP, has been implicated as both a downregulator and effector of Ras proteins, but its precise role in Ras-mediated signal transduction pathways is unclear. To begin a genetic analysis of the role of p120 Ras-GAP we identified a homolog from the fruit fly Drosophila melanogaster through its ability to complement the sterility of a Schizosaccharomyces pombe (fission yeast) gap1 mutant strain. Like its mammalian homolog, Drosophila RasGAP stimulated the intrinsic GTPase activity of normal mammalian H-Ras but not that of the oncogenic Val12 mutant. RasGAP was tyrosine phosphorylated in embryos and its Src homology 2 (SH2) domains could bind in vitro to a small number of tyrosine-phosphorylated proteins expressed at various developmental stages. Ectopic expression of RasGAP in the wing imaginal disc reduced the size of the adult wing by up to 45% and suppressed ectopic wing vein formation caused by expression of activated forms of Breathless and Heartless, two Drosophila receptor tyrosine kinases of the fibroblast growth factor receptor family. The in vivo effects of RasGAP overexpression required intact SH2 domains, indicating that intracellular localization of RasGAP through SH2-phosphotyrosine interactions is important for its activity. These results show that RasGAP can function as an inhibitor of signaling pathways mediated by Ras and receptor tyrosine kinases in vivo. Genetic interactions, however, suggested a Ras-independent role for RasGAP in the regulation of growth. The system described here should enable genetic screens to be performed to identify regulators and effectors of p120 Ras-GAP.  (+info)

Mammalian staufen is a double-stranded-RNA- and tubulin-binding protein which localizes to the rough endoplasmic reticulum. (5/28861)

Staufen (Stau) is a double-stranded RNA (dsRNA)-binding protein involved in mRNA transport and localization in Drosophila. To understand the molecular mechanisms of mRNA transport in mammals, we cloned human (hStau) and mouse (mStau) staufen cDNAs. In humans, four transcripts arise by differential splicing of the Stau gene and code for two proteins with different N-terminal extremities. In vitro, hStau and mStau bind dsRNA via each of two full-length dsRNA-binding domains and tubulin via a region similar to the microtubule-binding domain of MAP-1B, suggesting that Stau cross-links cytoskeletal and RNA components. Immunofluorescent double labeling of transfected mammalian cells revealed that Stau is localized to the rough endoplasmic reticulum (RER), implicating this RNA-binding protein in mRNA targeting to the RER, perhaps via a multistep process involving microtubules. These results are the first demonstration of the association of an RNA-binding protein in addition to ribosomal proteins, with the RER, implicating this class of proteins in the transport of RNA to its site of translation.  (+info)

Identification and characterization of the human orthologue of yeast Pex14p. (6/28861)

Pex14p is a central component of the peroxisomal protein import machinery, which has been suggested to provide the point of convergence for PTS1- and PTS2-dependent protein import in yeast cells. Here we describe the identification of a human peroxisome-associated protein (HsPex14p) which shows significant similarity to the yeast Pex14p. HsPex14p is a carbonate-resistant peroxisomal membrane protein with its C terminus exposed to the cytosol. The N terminus of the protein is not accessible to exogenously added antibodies or protease and thus might protrude into the peroxisomal lumen. HsPex14p overexpression leads to the decoration of tubular structures and mislocalization of peroxisomal catalase to the cytosol. HsPex14p binds the cytosolic receptor for the peroxisomal targeting signal 1 (PTS1), a result consistent with a function as a membrane receptor in peroxisomal protein import. Homo-oligomerization of HsPex14p or interaction of the protein with the PTS2-receptor or HsPex13p was not observed. This distinguishes the human Pex14p from its counterpart in yeast cells and thus supports recent data suggesting that not all aspects of peroxisomal protein import are conserved between yeasts and humans. The role of HsPex14p in mammalian peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible for an unrecognized complementation group of human peroxisome biogenesis disorders.  (+info)

Down-regulation of RpS21, a putative translation initiation factor interacting with P40, produces viable minute imagos and larval lethality with overgrown hematopoietic organs and imaginal discs. (7/28861)

Down-regulation of the Drosophila ribosomal protein S21 gene (rpS21) causes a dominant weak Minute phenotype and recessively produces massive hyperplasia of the hematopoietic organs and moderate overgrowth of the imaginal discs during larval development. Here, we show that the S21 protein (RpS21) is bound to native 40S ribosomal subunits in a salt-labile association and is absent from polysomes, indicating that it acts as a translation initiation factor rather than as a core ribosomal protein. RpS21 can interact strongly with P40, a ribosomal peripheral protein encoded by the stubarista (sta) gene. Genetic studies reveal that P40 underexpression drastically enhances imaginal disc overgrowth in rpS21-deficient larvae, whereas viable combinations between rpS21 and sta affect the morphology of bristles, antennae, and aristae. These data demonstrate a strong interaction between components of the translation machinery and showed that their underexpression impairs the control of cell proliferation in both hematopoietic organs and imaginal discs.  (+info)

DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines. (8/28861)

The Src homology 3 (SH3) motif is found in numerous signal transduction proteins involved in cellular growth and differentiation. We have purified and cloned a novel protein, DEF-1 (differentiation-enhancing factor), from bovine brain by using a Src SH3 affinity column. Ectopic expression of DEF-1 in fibroblasts resulted in the differentiation of a significant fraction of the culture into adipocytes. This phenotype appears to be related to the induction of the transcription factor peroxisome proliferator-activated receptor gamma (PPARgamma), since DEF-1 NIH 3T3 cells demonstrated augmented levels of PPARgamma mRNA and, when treated with activating PPARgamma ligands, efficient induction of differentiation. Further evidence for a role for DEF-1 in adipogenesis was provided by heightened expression of DEF-1 mRNA in adipose tissue isolated from obese and diabetes mice compared to that in tissue isolated from wild-type mice. However, DEF-1 mRNA was detected in multiple tissues, suggesting that the signal transduction pathway(s) in which DEF-1 is involved is not limited to adipogenesis. These results suggest that DEF-1 is an important component of a signal transduction process that is involved in the differentiation of fibroblasts and possibly of other types of cells.  (+info)

In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA ( ... Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library. When scientists ... "Cloning of Complementary DNA Encoding a Functional Human Interleukin-8 Receptor." Science (American Association for the ... "Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project." Science (American Association for the ...
copy DNA (cDNA) See complementary DNA. copy error A mutation resulting from a mistake made during DNA replication. copy-number ... A-DNA One of three main biologically active structural conformations of the DNA double helix, along with B-DNA and Z-DNA. The A ... Complementarity is fundamental to the mechanisms governing DNA replication, transcription, and DNA repair. complementary DNA ( ... either DNA-DNA, RNA-RNA, or a DNA-RNA hybrid) which are held together by hydrogen bonds between the complementary nucleobases ...
This results in the creation of RNA-DNA hybrids where a single strand of complementary DNA is bound to a strand of mRNA. To ... When studying eukaryotic DNA, expression libraries are constructed using complementary DNA (cDNA) to help ensure the insert is ... A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which ... DNA polymerase I is then added, the cleaved RNA acts as a primer the DNA polymerase I can identify and initiate replacement of ...
In DNA, fraying occurs when non-complementary regions exist at the end of an otherwise complementary double-strand of DNA. ... DNA exists in many possible conformations that include A-DNA, B-DNA, and Z-DNA forms, although only B-DNA and Z-DNA have been ... In DNA replication, DNA-dependent DNA polymerases make copies of DNA polynucleotide chains. To preserve biological information ... DNA at Curlie DNA binding site prediction on protein DNA the Double Helix Game From the official Nobel Prize web site DNA under ...
Crick, F. & Watson, J. D. (1954). "The Complementary Structure of Deoxyribonucleic Acid" (PDF). Proceedings of the Royal ... and Z-form DNA. Even once the DNA duplex structure was solved, it was initially an open question whether additional DNA ... The DNA double helix was discovered in 1953 (with further details in 1954) based on X-ray diffraction images of DNA (most ... Under torsional stress, a Z-DNA structure can form with opposite twist to B-form DNA, but this is rare within the cellular ...
Rudner, R; Karkas, JD; Chargaff, E (1968). "Separation of B. Subtilis DNA into complementary strands. 3. Direct analysis". ... During replication the DNA strands separate. In single stranded DNA, cytosine spontaneously slowly deaminates to adenosine (a C ... The second rule holds that both Α% ≈ Τ% and G% ≈ C% are valid for each of the two DNA strands. This describes only a global ... 3-In 1947, Chargaff believed the notion that DNA has a shape of a Mobius Strip *(which was proven to be false).* The first rule ...
Curriculum Vitae Hanukoglu I, Tanese N, Fuchs E (Feb 1983). "Complementary DNA sequence of a human cytoplasmic actin. ...
Hanukoglu I, Tanese N, Fuchs E (Feb 1983). "Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of ...
Cloning and sequence analysis of the complementary DNA". Biochemistry. 26 (13): 3975-82. doi:10.1021/bi00387a035. PMID 3651428 ...
Wang LH, Ohashi K, Wu KK (May 1991). "Isolation of partial complementary DNA encoding human thromboxane synthase". Biochemical ... and interaction of transcriptional regulatory factor with its DNA element in the 5' flanking region". Biochimica et Biophysica ...
"Nucleotide sequence of a complementary DNA for human ST2". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression ... "Nucleotide sequence of a complementary DNA for human ST2". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression ... Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 ...
"Sequence and expression of human estrogen receptor complementary DNA". Science. 231 (4742): 1150-1154. Bibcode:1986Sci... ... Teo AK, Oh HK, Ali RB, Li BF (October 2001). "The modified human DNA repair enzyme O(6)-methylguanine-DNA methyltransferase is ... "Sequence and expression of human estrogen receptor complementary DNA". Science. 231 (4742): 1150-1154. Bibcode:1986Sci... ... Thénot S, Bonnet S, Boulahtouf A, Margeat E, Royer CA, Borgna JL, Cavaillès V (December 1999). "Effect of ligand and DNA ...
Racaniello VR, Baltimore D (November 1981). "Cloned poliovirus complementary DNA is infectious in mammalian cells". Science. ... Scientists first converted poliovirus's published RNA sequence, 7741 bases long, into a DNA sequence, as DNA was easier to ... Enzymes were used to convert the DNA back into RNA, its natural state. Other enzymes were then used to translate the RNA into a ... DNA encoding the RNA genome of poliovirus was introduced into cultured mammalian cells and infectious poliovirus was produced. ...
"Cloning and sequence analysis of human calcyphosine complementary DNA". Biochim Biophys Acta. 1352 (3): 249-52. doi:10.1016/ ... 2004). "The DNA sequence and biology of human chromosome 19". Nature. 428 (6982): 529-35. Bibcode:2004Natur.428..529G. doi: ...
Production of complementary DNA representing RNA viral sequences by recombinant DNA methods and uses therefor, retrieved 2014- ... Racaniello, Vincent R.; David Baltimore (1981-11-20). "Cloned Poliovirus Complementary DNA is Infectious in Mammalian Cells". ... Racaniello used recombinant DNA technology to clone and sequence the genome of the small RNA animal virus poliovirus. Using ...
Miller JS, Moxley G, Schwartz LB (Oct 1990). "Cloning and characterization of a second complementary DNA for human tryptase". J ... Miller JS, Westin EH, Schwartz LB (1989). "Cloning and characterization of complementary DNA for human tryptase". J. Clin. ...
DNA is double-stranded containing both a sense strand and an antisense strand. Therefore, the complementary sequence will be to ... B-DNA is the most common form of DNA in vivo and is a more narrow, elongated helix than A-DNA. Its wide major groove makes it ... A-DNA, is a form of the DNA duplex observed under dehydrating conditions. It is shorter and wider than B-DNA. RNA adopts this ... Biology portal Biomolecular structure Crosslinking of DNA DNA nanotechnology DNA supercoil Gene structure Non-helical models of ...
Complementary DNA Cloning and Expression in Bacteria". Science. 205 (4406): 602-607. doi:10.1126/science.377496. Ullrich, Axel ... "Tumor DNA Structure in Plant Cells Transformed by A. tumefaciens". Science. 209 (4463): 1385-1391. doi:10.1126/science.6251546 ...
June 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. ... RNA is first copied as complementary DNA (cDNA) by a reverse transcriptase enzyme before the resultant cDNA is sequenced. ... Libraries of silkmoth mRNA transcripts were collected and converted to complementary DNA (cDNA) for storage using reverse ... Shalon D, Smith SJ, Brown PO (July 1996). "A DNA microarray system for analyzing complex DNA samples using two-color ...
June 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. ... In 1979 teams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of ... The general goal of the technique is similar to the DNA microarray. However, SAGE sampling is based on sequencing mRNA output, ... The cDNA concatemers can then be isolated and sequenced using modern high-throughput DNA sequencers, and these sequences can be ...
DNA polymerase incorporates the correct, complementary dNTPs onto the template. This incorporation releases pyrophosphate (PPi ... "Sequencing by synthesis" involves taking a single strand of the DNA to be sequenced and then synthesizing its complementary ... Essentially, the method allows sequencing a single strand of DNA by synthesizing the complementary strand along it, one base ... Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by ...
Because these clones consist of DNA that is complementary to mRNA, the ESTs represent portions of expressed genes. They may be ... Gene expression Complementary DNA (cDNA) transcriptomics IMAGE cDNA clones Whole genome sequencing (WGS) ESTs Factsheet. ... Jun 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. ... In 1979, teams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of ...
"Molecular analysis of human and rat calmodulin complementary DNA clones. Evidence for additional active genes in these species ...
Tn5 mediated cleavage produces a library of target DNA sites bound to a protein of interest in situ. Sequencing of prepared DNA ... This epigenetic information is complementary to genotype and expression analysis. CUT&Tag is an alternative to the current ... Competition-ChIP: Measures relative replacement dynamics on DNA. ChiRP-Seq: Measures RNA-bound DNA and proteins. ChIP-exo: ... DamID: Uses enrichment of methylated DNA sequences to detect protein-DNA interaction without antibodies. CUT&RUN: Uses protein ...
Complementary DNA can then be processed the same way as genomic DNA. After DNA or RNA extraction, samples may require further ... large DNA fragments into shorter DNA fragments. The fragmented DNA may then be cloned into a DNA vector and amplified in a ... DNA sequencing may be used along with DNA profiling methods for forensic identification and paternity testing. DNA testing has ... One key issue is the ownership of an individual's DNA and the data produced when that DNA is sequenced. Regarding the DNA ...
SenGupta B, Friedberg F, Detera-Wadleigh SD (December 1987). "Molecular analysis of human and rat calmodulin complementary DNA ... In the lab experiment with rats, lambda rCB1 or hCE1 underwent DNA isolation as both of the genes included the CALM-3 gene, and ... The CALM-3 gene, along with the protein of calmodulin, has been included in different types of experiments such as DNA ...
This transgenic complementary DNA enables production of orange pelargonidin pigment. One study of three commercially ... After analyzing a sample he discovered that the DNA of the flowers matched the modification described in the original 1987 ... Text taken from Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified ... "Event-specific qualitative polymerase chain reaction analysis for two T-DNA copies in genetically modified orange Petunia". ...
Evidence-Based Complementary and Alternative Medicine. 2017: 1456786. doi:10.1155/2017/1456786. PMC 5307125. PMID 28250789. ... "Flower Extracts Attenuate Proliferation and Induce Apoptosis in Malignant Cells through Inducing DNA Damage and Inhibiting ...
Miller JS, Moxley G, Schwartz LB (September 1990). "Cloning and characterization of a second complementary DNA for human ...
When a sample of unknown RNA is flowed over the array, the RNA base pairs with and binds to complementary DNA. Bound ... RNA sequencing (RNA-Seq) is performed by reverse transcribing RNA to complementary DNA (cDNA) and high-throughput sequencing ... DNA microarrays are solid surfaces, usually a small chip, to which short DNA polymers of known sequence are covalently bound. ... DNA spiking qPCR Yang IV (2006). "[4] Use of External Controls in Microarray Experiments". DNA Microarrays, Part B: Databases ...
In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA ( ... Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library. When scientists ... "Cloning of Complementary DNA Encoding a Functional Human Interleukin-8 Receptor." Science (American Association for the ... "Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project." Science (American Association for the ...
15.13: 互补DNA "只有转录成信使RNA(mRNA)的基因才是活跃的或表达的。因此,科学家可以从
Hairpin-bisulfite PCR: Assessing epigenetic methylation patterns on complementary strands of individual DNA molecules. ... Home » Hairpin-bisulfite PCR: Assessing epigenetic methylation patterns on complementary strands of individual DNA molecules ... Hairpin-bisulfite PCR: Assessing epigenetic methylation patterns on complementary strands of individual DNA molecules. ...
Heterochiral DNA with Complementary Strands in α-D and β-D Configuration: H-Bonded and Silver Mediated Base Pairs with Impact ... Heterochiral DNA with Complementary Strands in α-D and β-D Configuration: H-Bonded and Silver Mediated Base Pairs with Impact ... Heterochiral DNA with Complementary Strands in α-D and β-D Configuration: H-Bonded and Silver Mediated Base Pairs with Impact ... Heterochiral DNA with Complementary Strands in α-D and β-D Configuration: H-Bonded and Silver Mediated Base Pairs with Impact ...
Heterochiral DNA with Complementary Strands in α-D and β-D Configuration: H-Bonded and Silver Mediated Base Pairs with Impact ... Heterochiral DNA with Complementary Strands in α-D and β-D Configuration: H-Bonded and Silver Mediated Base Pairs with Impact ... Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an ... Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an ...
... followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching ... followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching ... Poly(A) Tailing and Complementary DNA Synthesis. We compared two molecular approaches [poly(A) tailing and RT-PCR] for ... end of fractionated 16S rRNA prior to synthesis of the full-length complementary DNA (cDNA; Botero et al., 2005). Since the ...
cDNA, complementary DNA; DENV, dengue virus.. Main Article. Page created: August 06, 2020 ...
One such technology is the cDNA (complementary DNA) library. The sheer number of companies offering premade and custom cDNA ... One such technology is the cDNA (complementary DNA) library. The sheer number of companies offering premade and custom cDNA ... One such technology is the cDNA (complementary DNA) library. The sheer number of companies offering premade and custom cDNA ... The term "transcriptome" refers to the set of genes that are expressed or transcribed from genomic DNA in a particular cell or ...
The G protein β2 complementary DNA encodes the β35 subunit. / Gao, B.; Mumby, S.; Gilman, A. G. In: Journal of Biological ... Gao, B. ; Mumby, S. ; Gilman, A. G. / The G protein β2 complementary DNA encodes the β35 subunit. In: Journal of Biological ... Gao, B., Mumby, S., & Gilman, A. G. (1987). The G protein β2 complementary DNA encodes the β35 subunit. Journal of Biological ... Gao, B, Mumby, S & Gilman, AG 1987, The G protein β2 complementary DNA encodes the β35 subunit, Journal of Biological ...
Complementary DNA for the mouse homolog of the small subunit of human cytochrome b558. / Sumimoto, Hideki; Nozaki, Masahiko; ... Complementary DNA for the mouse homolog of the small subunit of human cytochrome b558. In: Biochemical and Biophysical Research ... Sumimoto H, Nozaki M, Sasaki H, Takeshige K, Sakaki Y, Minakami S. Complementary DNA for the mouse homolog of the small subunit ... Sumimoto, H, Nozaki, M, Sasaki, H, Takeshige, K, Sakaki, Y & Minakami, S 1989, Complementary DNA for the mouse homolog of the ...
DNA, Complementary / genetics* * DNA, Complementary / metabolism * Dual Specificity Phosphatase 6 / isolation & purification ...
A DNA strand has the sequence ACCGAGCTT. Which is the complementary strand of RNA?. posted on December 16, 2021. ... A DNA strand has the sequence ACCGAGCTT. Which is the complementary strand of RNA?. ... The sequence of a dna strand is acctggat what will be the… ... Which mrna sequence is the complement to the dna sequence…. *. ...
complementary DNA. Advertisement - Remove. .adside { width: 300px; height: 250px; }@media(min-width: 468px) { .adside { width: ...
DNA, Complementary / genetics * Follow-Up Studies * Gene Expression Regulation * Giant Cell Arteritis / drug therapy ...
Complementary DNA cloning and southern hybridization analysis. M M Goldrick, M M Goldrick ... full-length complementary DNA (cDNA) copies were produced of L chain mRNA from the M75 myeloma that had been induced in the C. ... Complementary DNA cloning and southern hybridization analysis.. J Exp Med 1 August 1985; 162 (2): 713-728. doi: https://doi.org ... end of the cloned V kappa Ser cDNA was used in Southern hybridizations of restriction digests of liver DNA from a number of ...
The activities of the different plant extracts against oxidative DNA damage were in the range 0.13-1.60 μg/ml. Of the ten ... Finally, potentially significant oxidative DNA damage preventive activity and antioxidant activity were noted in three plant ... and capacity to prevent oxidative DNA damage. Cytotoxic activity was also determined against the U937 cell line. IC50 values ... scariosus showed significant potential for preventing oxidative DNA damage and radical scavenging activity, and the G. ...
These probes hybridize with complementary target DNA sequences [40] in the genome and can detect chromosomal abnormalities, ... DNA can be extracted from these cells for molecular analysis. DNA analysis of CVS specimens is helpful for early diagnosis of ... 8] Use of cell-free DNA to screen for other aneuploidies (trisomy 16 and 22), microdeletions, and expanded cell-free DNA genome ... they release the fetal portion of cell-free fetal DNA. Cell-free fetal DNA can be extracted from maternal blood starting at 9- ...
... focuses on evidence concerning the efficacy and safety of complementary medical (CM) whole systems, practices, interventions ... traditional medicines and their synthetic duplicates Efficacy and safety of complementary therapies Evidence-based medicine and ... practice, including evidence of traditional use Curriculum development, educational system and competency of complementary ... Objective Journal of Complementary and Integrative Medicine ( JCIM ) ...
5. Fragment of notes on a double helix model for DNA Date: [ca. 1953] ... Notes on the possible configuration of bases in the DNA structure Date: [ca. 1953] ... The Complementary Structure of Deoxyribonucleic Acid Date: 1954 19. The Complementary Structure of DNA Date: 1954 ...
Transcriptome analysis by strand-specific sequencing of complementary DNA. Nucleic Acids Res. 37, e123 (2009). ...
Derby #180: Complementary Colors. Derby #180: Complementary Colors. Hey, this Derbys over. When youre finished reliving past ... Complementary colors. So for this week your design must be made of two colors paired up in the list below. Before you freak out ...
... complementary DNA; CE, chilling exposure; EF1α, Elongation factor 1 alpha; FW, fresh weight; gDNA, genomic DNA; hpce/mpce/dpce ... Plasmid DNA was extracted from putative recombinant clones (Bimboim and Doly, 1979) and confirmed by using EcoRI restriction ... RACE-PCRs were performed using 0.5 U of Taq DNA polymerase (Thermo Scientific, Wilmington, DE, USA) with 1X manufacturer ... Total RNA from all samples was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), with on-column digestion of DNA ...
Work by our two groups is therefore highly complementary.) Overall, the state of the art in this area is clearly fast advancing ... Aim 2: to study replication-origin spacing and DNA synthesis speed at single-molecule resolution on DNA fibres, comparing these ... Thus, schizogony challenges some basic paradigms about DNA replication control, while gametogenesis demands a speed of DNA ... it is impossible to measure discrete tracks of nascent DNA replication because strands of DNA several 100kb long are completely ...
Genetically encoding DNA nanostructures provides a route for their production as well as applications in living cells. DNA ... Here the authors combine RNA hairpins and reverse transcription to generate and assemble a complex DNA structure inside the ... which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. Purified ssDNA that is ... The field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) ...
Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270, 467-470.Google Scholar ... The Absence of C-5 DNA Methylation in Leishmania donovani Allows DNA Enrichment from Complex Samples. Microorganisms, Vol. 8, ... Duncan, R. (2004). DNA microarray analysis of protozoan parasite gene expression: outcomes correlate with mechanisms of ... Evaluation of differential gene expression in Leishmania major Friedlin procyclics and metacyclics using DNA microarray ...
It acts as a traffic cop to steer repair to simple end-joining or to patching the cut with new, single-strand DNA. Scientists ... surprisingly plays a key role in repairing double-strand DNA breaks created by CRISPR-Cas9. ... could potentially tweak proteins involved in the pathway to preferentially steer the outcome toward replacement with DNA, which ... Scientists discovered that a well-known DNA repair pathway, the Fanconi anemia pathway, ...
... genomic DNA; cDNA, complementary DNA. ... Genome-Wide parent-of-origin DNA methylation analysis reveals ... Genome-Wide analysis of DNA methylation dynamics during early human development. PLoS Genet 2014;10:e1004868. doi:10.1371/ ... In embryonic stem cells, ZFP57/KAP1 recognize a methylated hexanucleotide to affect chromatin and DNA methylation of imprinting ... Methylation levels by pyrosequencing for the leucocyte genomic DNA from the patient (green dots), father (blue dots) and mother ...
Cloning and Characterization of a Complementary DNA Encoding a Human Epididymis-Associated Disintegrin and Metalloprotease 7 ... Molecular Biology of the Channel Catfish Gonadotropin Receptors: 2. Complementary DNA Cloning, Functional Expression, and ...
The DNA strands are self-complementary, which means they bond to one another. By tuning parameters like the length of the DNA ... However, the atoms in this crystal-spheres with an even coating of DNA-are the same in all directions, so they tend to build ... Smaller metal spheres, with fewer DNA strands to make them less sticky, end up acting like electrons in an arrangement of ... Mirkins team created colloidal crystals by coating metal nanoparticles with DNA to make them stick to one another. ...
The probes contain complementary sequences that hybridize to the target sequence. A polymerase then fills in the target ... Exonucleases remove the non-circularized probes and the off-target DNA that is not captured. Finally, PCR is done to amplify ... Evonetix Inks Deal With Analog Devices to Expand Thermally Controlled Enzymatic DNA Synthesis ...
  • In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA (miRNA)) template in a reaction catalyzed by the enzyme reverse transcriptase. (wikipedia.org)
  • cDNA is often used to express a specific protein in a cell that does not normally express that protein (i.e., heterologous expression), or to sequence or quantify mRNA molecules using DNA based methods (qPCR, RNA-seq). (wikipedia.org)
  • The term cDNA is also used, typically in a bioinformatics context, to refer to an mRNA transcript's sequence, expressed as DNA bases (deoxy-GCAT) rather than RNA bases (GCAU). (wikipedia.org)
  • Patentability of cDNA was a subject of a 2013 US Supreme Court decision in Association for Molecular Pathology v. Myriad Genetics, Inc. As a compromise, the Court declared, that exons-only cDNA is patent-eligible, whereas isolated sequences of naturally occurring DNA comprising introns are not. (wikipedia.org)
  • In cellular life, cDNA is generated by viruses and retrotransposons for integration of RNA into target genomic DNA. (wikipedia.org)
  • An optimization of this procedure relies on low RNase H activity of M-MLV to nick mRNA with remaining RNA later removed by adding RNase H after DNA Polymerase translation of the second-strand cDNA. (wikipedia.org)
  • Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library. (wikipedia.org)
  • When scientists transfer a gene from one cell into another cell in order to express the new genetic material as a protein in the recipient cell, the cDNA will be added to the recipient (rather than the entire gene), because the DNA for an entire gene may include DNA that does not code for the protein or that interrupts the coding sequence of the protein (e.g., introns). (wikipedia.org)
  • To obtain a better understanding of metabolically active microbial communities, we tested a molecular ecological approach using poly(A) tailing of environmental 16S rRNA, followed by full-length complementary DNA (cDNA) synthesis and sequencing to eliminate potential biases caused by mismatching of polymerase chain reaction (PCR) primer sequences. (frontiersin.org)
  • One such technology is the cDNA (complementary DNA) library. (the-scientist.com)
  • To further characterize this V kappa group and begin to analyze the basis for its strain-specific expression, full-length complementary DNA (cDNA) copies were produced of L chain mRNA from the M75 myeloma that had been induced in the C.C58 strain of mice, and which produces a V kappa Ser L chain. (silverchair.com)
  • A probe corresponding to 200 bp of the 5' end of the cloned V kappa Ser cDNA was used in Southern hybridizations of restriction digests of liver DNA from a number of inbred, recombinant, and recombinant inbred strains. (silverchair.com)
  • The Court specifically mentioned the ability to patent a type of DNA known as complementary DNA (cDNA). (medlineplus.gov)
  • The court said tests that involve making cDNA, or complementary DNA, can still be patented. (foxnews.com)
  • The probes contain complementary sequences that hybridize to the target sequence. (genomeweb.com)
  • The Supreme Court's ruling did allow that DNA manipulated in a lab is eligible to be patented because DNA sequences altered by humans are not found in nature. (medlineplus.gov)
  • Put exonic DNA sequences on a chip. (flashcardmachine.com)
  • Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
  • We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
  • With a few minor exceptions, the DNA sequence fits the previously published amino acid sequences. (rti.org)
  • The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)
  • The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)
  • ROESY, NOE spectroscopy in the operator DNA sequences aimed at removing the rotating frame. (lu.se)
  • DNA sequences used for studies of uncomplexed and complexed trp operator. (lu.se)
  • Heterochiral DNA with H-bonded and silver mediated base pairs was constructed utilizing complementary strands with nucleosides in α-D or β-D configuration. (cdna-clone.com)
  • The DNA strands are self-complementary, which means they bond to one another. (eurekalert.org)
  • Smaller metal spheres, with fewer DNA strands to make them less sticky, end up acting like electrons in an arrangement of larger nanoparticle 'atoms. (eurekalert.org)
  • These complementary strands transiently bind to each other. (mpg.de)
  • The great advantage of indirect binding of the DNA strands is that the interaction can be regulated via their DNA sequence, which is programmable. (mpg.de)
  • In short, by means of the exact construction of the strands, in other words the sequence of the DNA building blocks, researchers can regulate their binding affinity: If they are only weakly complementary, the strands quickly separate from each other and the signal disappears. (mpg.de)
  • A single string of DNA can fold into all kinds of intricate shapes-depending on the base sequence encoded in the staple strands. (thebulletin.org)
  • And because researchers were focused on designing structures from scratch, it was necessary to create purified DNA strands. (thebulletin.org)
  • Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
  • The temperature of these is increased to 90-95°C in order to separate (denature) the 2 strands of target DNA. (medscape.com)
  • On June 13, 2013, in the case of the Association for Molecular Pathology v. Myriad Genetics, Inc., the Supreme Court of the United States ruled that human genes cannot be patented in the U.S. because DNA is a "product of nature. (medlineplus.gov)
  • Patents held by Myriad Genetics Inc. on BRCA1 and BRCA2 genes are not valid, because isolating a naturally occurring segment of DNA cannot be patented. (foxnews.com)
  • The Gubler and Hoffman Procedure uses E. Coli RNase H to nick mRNA that is replaced with E. Coli DNA Polymerase I and sealed with E. Coli DNA Ligase. (wikipedia.org)
  • You use reverse transcriptase to make DNA copies of the mRNA. (flashcardmachine.com)
  • We report the molecular cloning of DNA complementary to human Hp mRNA. (rti.org)
  • That's because nanomaterials are just the right size to interact with important biological actors, such as proteins, DNA molecules, and viruses. (technologyreview.com)
  • The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
  • DNA preparations extracted from fecal samples are tested by PCR with diagnostic primers. (cdc.gov)
  • 1 The TaqMan probe is designed to be complementary to a specific sequence spanned by the PCR primers. (cdc.gov)
  • Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
  • In the second step, the temperature decreases (45-60°C), depending on the primers, to permit annealing (strengthening) of the target DNA primers. (medscape.com)
  • Refinement of single-nucleotide polymorphism genotyping methods on human genomic DNA: amplifluor allele-specific polymerase chain reaction versus ligation detection reaction-TaqMan. (mpg.de)
  • [ 1 ] The resultant complementary DNA is amplified by polymerase chain reaction (PCR). (medscape.com)
  • However, the desired nucleotides must be incorporated into a long DNA with complex secondary structure from which they would need to be cleaved as a second step. (nature.com)
  • DNA sequencing is useful for determining the order of the precise nucleotides in a DNA molecule. (medgadget.com)
  • Nucleotides for which water-mediated pro- tein-DNA contacts with the bases were observed in the co-crystal structures are encircled with a continuous line. (lu.se)
  • Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
  • Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
  • In real-time PCR, the DNA amplification is monitored by measuring the fluorescence signal generated in the reaction vessel. (cdc.gov)
  • Phylogenetic analysis with DNA amplification technologies. (who.int)
  • The result of first-strand syntheses, RNA-DNA hybrids, can be processed through multiple second-strand synthesis methods or processed directly in downstream assays. (wikipedia.org)
  • Here 8 male gametes are produced from a single 'gametocyte' in less than 10 minutes, necessitating extraordinarily rapid DNA synthesis. (europa.eu)
  • HTBS recruits HIV reverse transcriptase, which nucleates DNA synthesis and is aided in elongation by murine leukemia reverse transcriptase. (nature.com)
  • Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
  • Fragile-site mental retardation 1 gene ( FMR1 ) deoxyribonucleic acid (DNA) testing for Fragile X syndrome and premutation involvement as indicated. (medscape.com)
  • RNA is transcribed from genomic DNA in host cells and is extracted by first lysing cells then purifying RNA utilizing widely-used methods such as phenol-chloroform, silica column, and bead-based RNA extraction methods. (wikipedia.org)
  • The term "transcriptome" refers to the set of genes that are expressed or transcribed from genomic DNA in a particular cell or tissue. (the-scientist.com)
  • The real-time PCR assays for parasite detection at CDC use either the DNA-binding dye SYBR Green or sequence-specific TaqMan probes as fluorescence detection mechanisms. (cdc.gov)
  • Assays using SYBR Green can be easier and less expensive, but caution should be exercised since all double-stranded DNA is detected, including primer-dimers and other PCR artifacts. (cdc.gov)
  • The genotoxic effects in the lung fibroblast (V79) cell line were examined using two complementary assays: the comet assay and micronucleus (MN) test. (cdc.gov)
  • Ligation detection reaction-TaqMan procedure for single nucleotide polymorphism detection on genomic DNA. (mpg.de)
  • In molecular biology, RNA is purified from source material after genomic DNA, proteins and other cellular components are removed. (wikipedia.org)
  • To remove DNA and proteins, enzymes such as DNase and Proteinase K are used for degradation. (wikipedia.org)
  • Scientists could potentially tweak proteins involved in the pathway to preferentially steer the outcome toward replacement with DNA, which is important for gene therapy for hereditary diseases. (sciencedaily.com)
  • This synthetic DNA is produced from the molecule that serves as the instructions for making proteins (called messenger RNA). (medlineplus.gov)
  • Integrative medicine and health (IMH) and complementary and alternative medicine (CAM) include healing approaches and therapies that historically have not been included in conventional, mainstream Western medicine. (msdmanuals.com)
  • Overview of Dietary Supplements Dietary supplements are the most commonly used of all integrative, complementary, and alternative therapies, primarily because they are widely available, relatively inexpensive, and can be bought. (msdmanuals.com)
  • Gao, B , Mumby, S & Gilman, AG 1987, ' The G protein β 2 complementary DNA encodes the β 35 subunit ', Journal of Biological Chemistry , vol. 262, no. 36, pp. 17254-17257. (elsevierpure.com)
  • The telomeres are the DNA-protein structures at the ends of eukaryotic chromosomes, which serve to protect and stabilize the genome. (lu.se)
  • Enzymes can stitch the dangling ends back together, which often results in one or more bases -- the building blocks of DNA -- being added or deleted, disrupting the function of the gene. (sciencedaily.com)
  • The four bases that make up DNA-adenine, cytosine, guanine, and thymine-always pair up in the same way. (thebulletin.org)
  • DNA structure, as was early recognized from and DNA bases are water-mediated (Otwinowski ®ber-diffraction X-ray studies of the water-depen- et al. (lu.se)
  • Journal of Complementary and Integrative Medicine ( JCIM ) focuses on evidence concerning the efficacy and safety of complementary medical (CM) whole systems, practices, interventions and natural health products, including herbal and traditional medicines. (degruyter.com)
  • Complementary, alternative, and integrative medicine are terms often used interchangeably, but their meanings are different. (msdmanuals.com)
  • 1988). This observation stimulated further dence of the A to B-DNA conversion (Franklin & studies by biochemical and genetic methods (for Gosling, 1953). (lu.se)
  • Complementary DNA microarray analysis was performed for gene expression profiling. (thejns.org)
  • Complementary DNA cloning and southern hybridization analysis. (silverchair.com)
  • Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB ® has the solution for you. (neb.com)
  • A DNA strand has the sequence ACCGAGCTT. (soetrust.org)
  • Which is the complementary strand of RNA? (soetrust.org)
  • Scientists discovered that a well-known DNA repair pathway, the Fanconi anemia pathway, surprisingly plays a key role in repairing double-strand DNA breaks created by CRISPR-Cas9. (sciencedaily.com)
  • It acts as a traffic cop to steer repair to simple end-joining or to patching the cut with new, single-strand DNA. (sciencedaily.com)
  • Alternatively, other enzymes can patch the break with a single strand of DNA that matches the DNA sequence upstream and downstream of the cut. (sciencedaily.com)
  • A complementary DNA strand is created to complete the double-strand repair. (sciencedaily.com)
  • Transcriptome analysis by strand-specific sequencing of complementary DNA. (mpg.de)
  • When you fold a single strand of DNA, you must fix the folded lengths in place with another tiny piece of DNA. (thebulletin.org)
  • You keep folding the single strand of DNA again and again until it has formed a shape. (thebulletin.org)
  • Rather than designing a single-strand DNA from scratch, he turned to naturally occurring, ready-made pieces of DNA: viruses. (thebulletin.org)
  • Rothemund designed DNA staples that would bind to specific points along the "scaffold" strand of a virus. (thebulletin.org)
  • This caused the viral DNA strand to automatically fold into a predetermined, two-dimensional shape. (thebulletin.org)
  • This method can generate tens of billions of copies of a particular DNA fragment (the sequence of interest, DNA of interest, or target DNA) from a DNA extract (DNA template). (intechopen.com)
  • Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an Oil Production Facility. (cdna-clone.com)
  • PCR amplified fragments can be analyzed by using restriction fragment length polymorphisms (RFLP) or DNA sequencing if further characterization is needed. (cdc.gov)
  • DNA purification on homemade silica spin-columns. (mpg.de)
  • In addition, the DNA Ligase Selection Chart helps you select the optimal DNA ligase for various applications. (neb.com)
  • 5X enzyme concentration over standard T4 DNA Ligase, good for increasing reaction rates or for bulk users. (neb.com)
  • See additional notes for T4 DNA Ligase. (neb.com)
  • Hi-T4 DNA Ligase displays increased thermotolerance compared to T4 DNA Ligase. (neb.com)
  • Hi-T4 DNA Ligase can withstand temperatures as high as 45°C for extended periods (up to 72 hours) without any significant loss in activity. (neb.com)
  • Salt-T4 DNA Ligase will join cohesive end termini at salt concentrations as high as 300 mM without any loss in activity. (neb.com)
  • Salt-T4 DNA Ligase is insensitive to salt carried over from other reaction components (vector or insert DNA) and allows ligation reactions to proceed in alternative reaction buffers with higher levels of salt (e.g. (neb.com)
  • See also notes for T4 DNA Ligase. (neb.com)
  • Since the discovery of the DNA double helix 65 years ago, researchers have developed a detailed understanding of this molecular-scale building material and its properties. (thebulletin.org)
  • Click to view the DNA extraction protocols recommended for molecular diagnosis of intestinal parasites. (cdc.gov)
  • Amplified DNA fragments are electrophoretically resolved on an agarose gel for analysis of results. (cdc.gov)
  • DNA origami: Unfolding risk? (thebulletin.org)
  • This area of biotechnology research, known as DNA origami, folds DNA into nano-scale shapes designed to perform specific mechanical functions or biological interactions. (thebulletin.org)
  • DNA's strict base-pairing rule and its self-assembly principle are what make it possible to design and create DNA origami structures. (thebulletin.org)
  • The arrival of DNA origami was announced to the world in 2006 when Rothemund's smiley face graced the cover of Nature . (thebulletin.org)
  • In 2012, another version of DNA origami was introduced. (thebulletin.org)
  • The labeled DNA probe combines with amplicon to form stable RNA: DNA hybrids. (cdc.gov)
  • During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). (cdc.gov)
  • Quantification of cyclin B1 and p34(cdc2) in bovine cumulus-oocyte complexes and expression mapping of genes involved in the cell cycle by complementary DNA macroarrays. (cdna-clone.com)
  • To find out which DNA repair enzymes are critical to homology-directed repair after CRISPR cutting, Richardson and Corn employed a technique called CRISPR interference (CRISPRi) to knock out, one at a time, more than 2,000 genes known or suspected to be involved in DNA repair, a function critical to a healthy cell. (sciencedaily.com)
  • Gene coexpression analysis refers to the en masse discovery of coexpressed genes from a large variety of transcriptomic experiments. (mdpi.com)
  • Then compare the production by tumor and regular cells as they hybridize to the exonic DNA sequence on the chip. (flashcardmachine.com)
  • Exonucleases remove the non-circularized probes and the off-target DNA that is not captured. (genomeweb.com)
  • The amplified segment may be observed using electrophoretic gel or can be identified by Southern blot analysis, using specific DNA probes for that segment. (medscape.com)
  • Like conventional techniques, the DNA-PAINT method uses fluorescent dyes as markers of cellular structures. (mpg.de)
  • Complementary medicine refers to non-mainstream practices used together with conventional medicine. (msdmanuals.com)
  • The ability to create nanostructures within living cells using DNA has the potential to be a powerful tool for basic biology, biomedical engineering and medicine ( Fig. 1a ). (nature.com)
  • In the latest example, University of California, Berkeley, scientists found that people's assumptions about how cells repair the genome after the Cas9 enzyme snips DNA are wrong. (sciencedaily.com)
  • DNA and RNA-based sequencing of the 16S rRNA gene and transcripts had been used to evaluate the phylogenetic range of microbial communities at property experiencing corrosion in an oil manufacturing facility. (cdna-clone.com)
  • Market Research Future (MRFR) recently launched a report on DNA Sequencing Market Research Report - Forecast to 2023, that declares this market could see an expansion at 17.64 % CAGR between 2018 and 2023. (medgadget.com)
  • In PCR, a thermostable DNA polymerase is used to amplify target DNA 2-fold with each temperature cycle. (medscape.com)
  • It is necessary to extract DNA from the stool specimens for PCR detection. (cdc.gov)
  • Contamination of reagents or specimens with target DNA from the environment must be avoided because such contamination can produce false-positive results. (medscape.com)
  • This project will reveal, with unprecedented resolution, how DNA replication is organised in Plasmodium and how it can be affected by changing conditions in the human host and exposure to antimalarial drugs. (europa.eu)
  • Thus, schizogony challenges some basic paradigms about DNA replication control, while gametogenesis demands a speed of DNA replication and cell division that is unprecedented in eukaryotic gametogenesis. (europa.eu)
  • This project is elucidating the spatio-temporal dynamics of DNA replication in these contrasting cell cycles. (europa.eu)
  • To do this, I have developed a method for labelling nascent DNA replication, which was not hitherto possible in Plasmodium. (europa.eu)
  • The world of COMPLEMENTARY MEDICINE is an infoholic's paradise. (infoholix.net)
  • Within the pages of this FREE DIRECTORY of COMPLEMENTARY MEDICINE you will discover worldwide connections to many different forms of healing and enlightenment. (infoholix.net)
  • An important market driver of the global DNA sequencing market size is a rise in adoption of precision medicine. (medgadget.com)
  • Exome sequencing from nanogram amounts of starting DNA: comparing three approaches. (mpg.de)
  • Specific DNA present in small amounts in a clinical specimen are amplified by PCR so they become detectable. (medscape.com)
  • CRISPR-Cas9 is revolutionary because of the precision with which it homes in on a specific DNA sequence out of billions in the genome and cleaves the double-stranded DNA molecule. (sciencedaily.com)
  • A gene patent is the exclusive rights to a specific sequence of DNA (a gene) given by a government to the individual, organization, or corporation who claims to have first identified the gene. (medlineplus.gov)
  • CH-8091 ZuÈrich, Switzerland uncomplexed trp operator DNA duplex. (lu.se)
  • DNA, serological detection of variola virus, antiviral agents and animal models of smallpox. (who.int)
  • Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
  • This approach depends on "bricks" rather than scaffolds-that is, small lengths of DNA are assembled into units reminiscent of Legos, which can in turn be joined together. (thebulletin.org)
  • In this area, another innovation of Jungmann's research work might come into play: Together with his colleagues, the biophysicist has developed tiny, three-dimensional DNA cages, which are self-assembling, extremely stable and could serve to deliver drugs. (mpg.de)
  • DNA damage and MN induction were found after exposure to all tested materials with the strongest effect seen for CNF. (cdc.gov)
  • The field of DNA nanotechnology has harnessed the programmability of DNA base pairing to direct single-stranded DNAs (ssDNAs) to assemble into desired 3D structures. (nature.com)
  • However, the 'atoms' in this crystal-spheres with an even coating of DNA-are the same in all directions, so they tend to build symmetric structures. (eurekalert.org)
  • And it could help researchers boost the efficiency with which cells insert new DNA into the genome -- to replace a harmful mutation with the correct DNA sequence, for example -- and generally tweak CRISPR-Cas9 editing to get the desired outcome. (sciencedaily.com)
  • Researchers often supply a single-stranded piece of DNA and hope that the cell uses it to replace the faulty sequence with the new one. (sciencedaily.com)
  • In a seemingly unrelated area of technology, researchers are designing and building machines out of DNA-at a scale one thousand times smaller than a human hair. (thebulletin.org)
  • Thus researchers can predict, with reasonably good success, the shapes into which a DNA molecule of a given sequence will fold. (thebulletin.org)