Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Crowdsourcing: Social media model for enabling public involvement and recruitment in participation. Use of social media to collect feedback and recruit volunteer subjects.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Dictionaries, ChemicalVocabulary, Controlled: A specified list of terms with a fixed and unalterable meaning, and from which a selection is made when CATALOGING; ABSTRACTING AND INDEXING; or searching BOOKS; JOURNALS AS TOPIC; and other documents. The control is intended to avoid the scattering of related subjects under different headings (SUBJECT HEADINGS). The list may be altered or extended only by the publisher or issuing agency. (From Harrod's Librarians' Glossary, 7th ed, p163)Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Research Support, Non-U.S. Gov'tOligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Research Support, U.S. GovernmentResearch Support, American Recovery and Reinvestment ActResearch Support, N.I.H., ExtramuralRNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)High-Throughput Nucleotide Sequencing: Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc.Psychology, Experimental: The branch of psychology which seeks to learn more about the fundamental causes of behavior by studying various psychologic phenomena in controlled experimental situations.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Vitamin K 3: A synthetic naphthoquinone without the isoprenoid side chain and biological activity, but can be converted to active vitamin K2, menaquinone, after alkylation in vivo.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Phonetics: The science or study of speech sounds and their production, transmission, and reception, and their analysis, classification, and transcription. (Random House Unabridged Dictionary, 2d ed)Language: A verbal or nonverbal means of communicating ideas or feelings.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Nasopharyngeal Neoplasms: Tumors or cancer of the NASOPHARYNX.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Cell Line, Tumor: A cell line derived from cultured tumor cells.Carcinoma: A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.ReadingPlant Physiological Phenomena: The physiological processes, properties, and states characteristic of plants.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Plant Development: Processes orchestrated or driven by a plethora of genes, plant hormones, and inherent biological timing mechanisms facilitated by secondary molecules, which result in the systematic transformation of plants and plant parts, from one stage of maturity to another.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Pharmacovigilance: The detection of long and short term side effects of conventional and traditional medicines through research, data mining, monitoring, and evaluation of healthcare information obtained from healthcare providers and patients.DNA Barcoding, Taxonomic: Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".Complementary Therapies: Therapeutic practices which are not currently considered an integral part of conventional allopathic medical practice. They may lack biomedical explanations but as they become better researched some (PHYSICAL THERAPY MODALITIES; DIET; ACUPUNCTURE) become widely accepted whereas others (humors, radium therapy) quietly fade away, yet are important historical footnotes. Therapies are termed as Complementary when used in addition to conventional treatments and as Alternative when used instead of conventional treatment.Metals, Heavy: Metals with high specific gravity, typically larger than 5. They have complex spectra, form colored salts and double salts, have a low electrode potential, are mainly amphoteric, yield weak bases and weak acids, and are oxidizing or reducing agents (From Grant & Hackh's Chemical Dictionary, 5th ed)Benchmarking: Method of measuring performance against established standards of best practice.Treatment Refusal: Patient or client refusal of or resistance to medical, psychological, or psychiatric treatment. (APA, Thesaurus of Psychological Index Terms, 8th ed.)Patient Compliance: Voluntary cooperation of the patient in following a prescribed regimen.Granulocyte-Macrophage Colony-Stimulating Factor: An acidic glycoprotein of MW 23 kDa with internal disulfide bonds. The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.Liver Transplantation: The transference of a part of or an entire liver from one human or animal to another.Immunocompromised Host: A human or animal whose immunologic mechanism is deficient because of an immunodeficiency disorder or other disease or as the result of the administration of immunosuppressive drugs or radiation.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Mice, Inbred C57BLImmunosuppressive Agents: Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.Aptamers, Nucleotide: Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.Electrochemical Techniques: The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues.Biosensing Techniques: Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.Computer Security: Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.Confidentiality: The privacy of information and its protection against unauthorized disclosure.Electrodes: Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.Electrochemistry: The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.

Regulation of body length and male tail ray pattern formation of Caenorhabditis elegans by a member of TGF-beta family. (1/28861)

We have identified a new member of the TGF-beta superfamily, CET-1, from Caenorhabditis elegans, which is expressed in the ventral nerve cord and other neurons. cet-1 null mutants have shortened bodies and male tail abnormal phenotype resembling sma mutants, suggesting cet-1, sma-2, sma-3 and sma-4 share a common pathway. Overexpression experiments demonstrated that cet-1 function requires wild-type sma genes. Interestingly, CET-1 appears to affect body length in a dose-dependent manner. Heterozygotes for cet-1 displayed body lengths ranging between null mutant and wild type, and overexpression of CET-1 in wild-type worms elongated body length close to lon mutants. In male sensory ray patterning, lack of cet-1 function results in ray fusions. Epistasis analysis revealed that mab-21 lies downstream and is negatively regulated by the cet-1/sma pathway in the male tail. Our results show that cet-1 controls diverse biological processes during C. elegans development probably through different target genes.  (+info)

Molecular cloning and epitope analysis of the peanut allergen Ara h 3. (2/28861)

Peanut allergy is a significant IgE-mediated health problem because of the increased prevalence, potential severity, and chronicity of the reaction. Following our characterization of the two peanut allergens Ara h 1 and Ara h 2, we have isolated a cDNA clone encoding a third peanut allergen, Ara h 3. The deduced amino acid sequence of Ara h 3 shows homology to 11S seed-storage proteins. The recombinant form of this protein was expressed in a bacterial system and was recognized by serum IgE from approximately 45% of our peanut-allergic patient population. Serum IgE from these patients and overlapping, synthetic peptides were used to map the linear, IgE-binding epitopes of Ara h 3. Four epitopes, between 10 and 15 amino acids in length, were found within the primary sequence, with no obvious sequence motif shared by the peptides. One epitope is recognized by all Ara h 3-allergic patients. Mutational analysis of the epitopes revealed that single amino acid changes within these peptides could lead to a reduction or loss of IgE binding. By determining which amino acids are critical for IgE binding, it might be possible to alter the Ara h 3 cDNA to encode a protein with a reduced IgE-binding capacity. These results will enable the design of improved diagnostic and therapeutic approaches for food-hypersensitivity reactions.  (+info)

ETO-2, a new member of the ETO-family of nuclear proteins. (3/28861)

The t(8;21) is associated with 12-15% of acute myelogenous leukemias of the M2 subtype. The translocation results in the fusion of two genes, AML1 (CBFA2) on chromosome 21 and ETO (MTG8) on chromosome 8. AML1 encodes a DNA binding factor; the ETO protein product is less well characterized, but is thought to be a transcription factor. Here we describe the isolation and characterization of ETO-2, a murine cDNA that encodes a new member of the ETO family of proteins. ETO-2 is 75% identical to murine ETO and shares very high sequence identities over four regions of the protein with ETO (domain I-III and zinc-finger). Northern analysis identifies ETO-2 transcripts in many of the murine tissues analysed and in the developing mouse embryo. ETO-2 is also expressed in myeloid and erythroid cell lines. We confirmed the nuclear localization of ETO-2 and demonstrated that domain III and the zinc-finger region are not required for nuclear localization. We further showed that a region within ETO, containing domain II, mediates dimerization among family members. This region is conserved in the oncoprotein AML-1/ETO. The recent identification of another ETO-like protein, myeloid translocation gene-related protein 1, together with the data presented here, demonstrates that at least three ETO proteins exist with the potential to form dimers in the cell nucleus.  (+info)

Control of growth and differentiation by Drosophila RasGAP, a homolog of p120 Ras-GTPase-activating protein. (4/28861)

Mammalian Ras GTPase-activating protein (GAP), p120 Ras-GAP, has been implicated as both a downregulator and effector of Ras proteins, but its precise role in Ras-mediated signal transduction pathways is unclear. To begin a genetic analysis of the role of p120 Ras-GAP we identified a homolog from the fruit fly Drosophila melanogaster through its ability to complement the sterility of a Schizosaccharomyces pombe (fission yeast) gap1 mutant strain. Like its mammalian homolog, Drosophila RasGAP stimulated the intrinsic GTPase activity of normal mammalian H-Ras but not that of the oncogenic Val12 mutant. RasGAP was tyrosine phosphorylated in embryos and its Src homology 2 (SH2) domains could bind in vitro to a small number of tyrosine-phosphorylated proteins expressed at various developmental stages. Ectopic expression of RasGAP in the wing imaginal disc reduced the size of the adult wing by up to 45% and suppressed ectopic wing vein formation caused by expression of activated forms of Breathless and Heartless, two Drosophila receptor tyrosine kinases of the fibroblast growth factor receptor family. The in vivo effects of RasGAP overexpression required intact SH2 domains, indicating that intracellular localization of RasGAP through SH2-phosphotyrosine interactions is important for its activity. These results show that RasGAP can function as an inhibitor of signaling pathways mediated by Ras and receptor tyrosine kinases in vivo. Genetic interactions, however, suggested a Ras-independent role for RasGAP in the regulation of growth. The system described here should enable genetic screens to be performed to identify regulators and effectors of p120 Ras-GAP.  (+info)

Mammalian staufen is a double-stranded-RNA- and tubulin-binding protein which localizes to the rough endoplasmic reticulum. (5/28861)

Staufen (Stau) is a double-stranded RNA (dsRNA)-binding protein involved in mRNA transport and localization in Drosophila. To understand the molecular mechanisms of mRNA transport in mammals, we cloned human (hStau) and mouse (mStau) staufen cDNAs. In humans, four transcripts arise by differential splicing of the Stau gene and code for two proteins with different N-terminal extremities. In vitro, hStau and mStau bind dsRNA via each of two full-length dsRNA-binding domains and tubulin via a region similar to the microtubule-binding domain of MAP-1B, suggesting that Stau cross-links cytoskeletal and RNA components. Immunofluorescent double labeling of transfected mammalian cells revealed that Stau is localized to the rough endoplasmic reticulum (RER), implicating this RNA-binding protein in mRNA targeting to the RER, perhaps via a multistep process involving microtubules. These results are the first demonstration of the association of an RNA-binding protein in addition to ribosomal proteins, with the RER, implicating this class of proteins in the transport of RNA to its site of translation.  (+info)

Identification and characterization of the human orthologue of yeast Pex14p. (6/28861)

Pex14p is a central component of the peroxisomal protein import machinery, which has been suggested to provide the point of convergence for PTS1- and PTS2-dependent protein import in yeast cells. Here we describe the identification of a human peroxisome-associated protein (HsPex14p) which shows significant similarity to the yeast Pex14p. HsPex14p is a carbonate-resistant peroxisomal membrane protein with its C terminus exposed to the cytosol. The N terminus of the protein is not accessible to exogenously added antibodies or protease and thus might protrude into the peroxisomal lumen. HsPex14p overexpression leads to the decoration of tubular structures and mislocalization of peroxisomal catalase to the cytosol. HsPex14p binds the cytosolic receptor for the peroxisomal targeting signal 1 (PTS1), a result consistent with a function as a membrane receptor in peroxisomal protein import. Homo-oligomerization of HsPex14p or interaction of the protein with the PTS2-receptor or HsPex13p was not observed. This distinguishes the human Pex14p from its counterpart in yeast cells and thus supports recent data suggesting that not all aspects of peroxisomal protein import are conserved between yeasts and humans. The role of HsPex14p in mammalian peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible for an unrecognized complementation group of human peroxisome biogenesis disorders.  (+info)

Down-regulation of RpS21, a putative translation initiation factor interacting with P40, produces viable minute imagos and larval lethality with overgrown hematopoietic organs and imaginal discs. (7/28861)

Down-regulation of the Drosophila ribosomal protein S21 gene (rpS21) causes a dominant weak Minute phenotype and recessively produces massive hyperplasia of the hematopoietic organs and moderate overgrowth of the imaginal discs during larval development. Here, we show that the S21 protein (RpS21) is bound to native 40S ribosomal subunits in a salt-labile association and is absent from polysomes, indicating that it acts as a translation initiation factor rather than as a core ribosomal protein. RpS21 can interact strongly with P40, a ribosomal peripheral protein encoded by the stubarista (sta) gene. Genetic studies reveal that P40 underexpression drastically enhances imaginal disc overgrowth in rpS21-deficient larvae, whereas viable combinations between rpS21 and sta affect the morphology of bristles, antennae, and aristae. These data demonstrate a strong interaction between components of the translation machinery and showed that their underexpression impairs the control of cell proliferation in both hematopoietic organs and imaginal discs.  (+info)

DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines. (8/28861)

The Src homology 3 (SH3) motif is found in numerous signal transduction proteins involved in cellular growth and differentiation. We have purified and cloned a novel protein, DEF-1 (differentiation-enhancing factor), from bovine brain by using a Src SH3 affinity column. Ectopic expression of DEF-1 in fibroblasts resulted in the differentiation of a significant fraction of the culture into adipocytes. This phenotype appears to be related to the induction of the transcription factor peroxisome proliferator-activated receptor gamma (PPARgamma), since DEF-1 NIH 3T3 cells demonstrated augmented levels of PPARgamma mRNA and, when treated with activating PPARgamma ligands, efficient induction of differentiation. Further evidence for a role for DEF-1 in adipogenesis was provided by heightened expression of DEF-1 mRNA in adipose tissue isolated from obese and diabetes mice compared to that in tissue isolated from wild-type mice. However, DEF-1 mRNA was detected in multiple tissues, suggesting that the signal transduction pathway(s) in which DEF-1 is involved is not limited to adipogenesis. These results suggest that DEF-1 is an important component of a signal transduction process that is involved in the differentiation of fibroblasts and possibly of other types of cells.  (+info)

Email: [email protected] We have conducted a human cDNA sequencing project for identification of unknown human transcripts in the past 8 years. A distinctive point of our project from other cDNA sequencing projects is in that we have focused our sequencing efforts on long cDNA clones (,4 kb), particularly those encoding large proteins in brain. This approach has brought us a unique cDNA resource consisting of large cDNA clones for newly identified human genes, which are known as KIAA cDNAs. The number of KIAA cDNAs has exceeded 2000 this year (the total number of the sequenced nucleotide residues, about 10 Mb) and we have made them publicly available. Because the number of genes encoding large proteins (,100 kDa) is always smaller than 10% of the total number of genes in eukaryotes, the number of human genes encoding large proteins is likely less than 3000. If this estimate is correct, we must have certainly entered endgame of the identification of human transcribed sequences expressed in the ...
Gene profiling was performed using a PCR-based subtractive hybridisation method, called Representation Difference Analysis (RDA), and kidney tissue from congenic versus hypertensive controls for the SHRSPGla. The RDA method itself I have also refined using glycerol-enhanced mini-polyacrylamide gel electrophoresis to optimize the separation of differentially expressed RDA fragments. I have isolated 20 such fragments that are differentially expressed and have been able to positively identify them by BLASTA search against known sequences on Genbank. These include the cytochrome P450 4A3 gene, which has been well documented as differentially expressed between SHR and WKY kidneys, and the low affinity Na-dependent glucose transporter (SGLT2). The expression of SGLT2 mRNA has been shown to be kidney specific and I have shown that it is differentially expressed in the congenic strain of SP.WKYGla2a versus the parental SHRSPGla. Two novel genes have also been mapped onto our target chromosome 2 within ...
www.MOLUNA.de Differential Display Methods and Protocols [4221492] - Part I. MethodologiesnGlobal Analysis of Gene Expression by Differential Display: A Mathematical ModelnShitao Yang and Peng LiangnAutomation of Fluorescent Differential Display With Digital ReadoutnJonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, and Peng LiangnOrdered Differential DisplaynMikhail V. Matz and Ella A. MeleshkevitchnGeneCalling®: Transcript Profiling Coupled
Synthesize full-length cDNA from ng of total RNA. Easy and efficient process maintains true gene representation. cDNA is immediately ready for downstream applications such as PCR, cloning, library construction & subtractive hybridization.
Synthesize full-length cDNA from ng of total RNA. Easy and efficient process maintains true gene representation. cDNA is immediately ready for downstream applications such as PCR, cloning, library construction & subtractive hybridization.
Verification result of the assembled full-length cDNA. Lane M represents the DL 2000 DNA size marker (Takara). Lane 1 represents the overlapping 3 end cDNA a
Definition of complementary DNA - synthetic DNA in which the sequence of bases is complementary to that of a given example of DNA.
有许多人说克隆是一件痛苦的工作,或许,有更多人的感觉使用Takara-Clontech产品进行克隆是一种愉悦。无论您是利用Takara经典的pMD系列进行传统克隆方法,还是使用In-fusion进行的新一代克隆操作;无论您是使用Takara经典的PrimeScript进行cDNA合成,还是使用SMARTer技术进行的新一代cDNA合成操作,Takara-Clontech带给您的都将是舒心、欣喜、兴奋 ...
有许多人说克隆是一件痛苦的工作,或许,有更多人的感觉使用Takara-Clontech产品进行克隆是一种愉悦。无论您是利用Takara经典的pMD系列进行传统克隆方法,还是使用In-fusion进行的新一代克隆操作;无论您是使用Takara经典的PrimeScript进行cDNA合成,还是使用SMARTer技术进行的新一代cDNA合成操作,Takara-Clontech带给您的都将是舒心、欣喜、兴奋 ...
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.. In our series of projects for accumulating sequence information on the coding sequences of unidentified human genes, we have newly determined the sequences of 100 cDNA clones from a set of size-fractionated human brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA0711 to KIAA0810. These cDNA clones were selected according to their coding potentials of large proteins (50 kDa and more) in vitro. The average sizes of the inserts and corresponding open reading frames were 4.3 kb and 2.6 kb (869 amino acid residues), respectively. Sequence analyses against the public databases indicated that the predicted coding sequences of 78 genes were similar to those of known genes, 64% of which (50 genes) were categorized as proteins functionally related to cell signaling/communication, cell structure/motility and nucleic acid management. As additional information ...
Gentaur molecular products has all kinds of products like :search , GenWay \ Full-length cDNA clone CS0DI023YE22 of Placenta of Homo sapiens - human \ 10-288-22076F for more molecular products just contact us
J:60127 Pascolo S, Tsoukatou D, Mamalaki C, Identification of thymus specific and developmentally regulated genes by an improved version of the mRNA differential display technique. Dev Immunol. 1999;7(1):1-7 ...
Nagase T، Seki N، Tanaka A، Ishikawa K، Nomura N (August 1995). "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1". DNA Research. 2 (4): 167-74, 199-210. PMID 8590280. doi:10.1093/dnares/2.4.167. ...
Gentaur molecular products has all kinds of products like :search , GeneRiver \ Heart cDNA \ CDHU002 for more molecular products just contact us
P-57. Generating and Sequencing Full-length cDNAs of Novel Human Genes Within the German cDNA Consortium. Ruth E. Wellenreuther, Stefan Wiemann, Daniela Heiss, Nina Claudino, Annemarie Poustka, Department of Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Heidelberg, GERMANY. We generate human cDNA libraries that are enriched in full length clones i.e. from the translation start to the poly A tail. These libraries are used for a) systematic sequencing within the cDNA consortium of the Genome Project aiming at the identification and analysis of as many new genes as possible and b) for screening to isolate full length clones of partial genes. Libraries are created by directional cloning of cDNAs into plasmid vectors. Full-length enrichment is achieved via Clontechs SMART technology. This method is PCR-based, and in our modified strategy, we amplify and clone selective size windows of the cDNA fraction above 3 kb.Clones from the libraries generated within this project are ...
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the ...
EasyScript® First-Strand cDNA Synthesis SuperMix,RT-PCR,PCR, RT-PCR, qPCR, qRT-PCR,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionEasyScript® First-Strand cDNA
Information about approximately 38,000 full-length cDNA clones that were completely sequenced in the Rice full-length cDNA project is shown in the database. The full-length cDNA clones were collected from various tissues treated under various stress conditions. The database contains not only information about complete nucleotide sequences and encoded amino acid sequences, but also results of homology searches against public databases, mapping information, information about patterns of alternative splicing, protein domains and transmembrane structures, and information about cellular localizations and gene functions annotated with Gene Ontology.. ...
4 Genome Science Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan. E-mail: [email protected] Functional annotation of DNA sequences is recognized as one of the most important processes in genome analysis. In this poster, we present an analysis of detecting functional motifs from the mouse full-length cDNA data, which are being sequenced by RIKEN. Our method detects functional motifs as commonly conserved regions between given cDNA sequences. Detection of such regions may give useful information for the functional assignment of cDNA sequences if the regions can be related with specific functions.. Our method mainly consists of the following two steps, both of which are based on the graph theory.. (1) Clustering of sequences: First, we compute all pairwise similarities of given protein sequences (which are translated from full-length cDNA sequences), and construct a linkage graph. In the graph, a vertex corresponds to a protein, and an edge is weighted by the ...
Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro ...
Histone H2B type 2-F is a protein that in humans is encoded by the HIST2H2BF gene. GRCh38: Ensembl release 89: ENSG00000203814 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000105827 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". "Entrez Gene: HIST2H2BF histone cluster 2, H2bf". Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMC 139241 . PMID 12477932. Cheung WL, Ajiro K, Samejima K, et al. (2003). "Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase". Cell. 113 (4): 507-17. doi:10.1016/S0092-8674(03)00355-6. PMID 12757711. Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMC ...
Build: Sat Nov 17 23:53:08 EST 2018 (commit: a759bb7). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
Bcl-2-associated transcription factor 1 is a Bcl-2 family protein in humans that is encoded by the BCLAF1 gene. This gene encodes a transcriptional repressor that interacts with several members of the BCL-2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different protein isoforms have been found for this gene. GRCh38: Ensembl release 89: ENSG00000029363 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000037608 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Nagase T, Seki N, Ishikawa K, Tanaka A, Nomura N (Nov 1996). "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell ...
... on testing of fractions of organic samples in bacterial development inhibition assays. goals. had been at that correct period just obtainable seeing that clones from a cDNA collection. 5 The overall idea was to create and characterize this peptide without prior direct BINA chemical or isolation synthesis. AMP Production Because of the little BINA bit of Rabbit polyclonal to PLSCR1. genuine peptide directly retrieved from isolates additional studies always rely on a technique to recover even more of the materials of interest. That is essential to exploit their setting of actions and their pharmaceutical potential. Since it converted out in addition it is among the larger challenges when learning more technical AMPs. Generally you can find three different techniques that may be used: immediate isolation of peptides from organic sources chemical substance synthesis or recombinant manifestation of peptides in transgenic ...
A variation on differential display, restriction‐mediated differential display (RMDD) presents an alternative approach to the fragment display technologies
x-charset gb2312,Hi, All, Does anybody knows how to access Rikens(Japan) full length Arabidopsis cDNA? There is a page maintained at http://www.gsc.riken.go.jp/Plant/database/index.html. Awfully, it does not contain a serch engine or Tools like Blast or Fasta etc.And it obviously is not a complete package. I would like to download the cDNA sequences. Thanks for your kindly inputs. Baiqing LIN --- ,/x-charset ...
To whom it may concern, I am looking for a cDNA expression library of wheat or barley made of reproductive organs, preferably reproductive buds. Please contact. Thanks in advance, Avi. ----------------------------------------------------------------------- Avi Golan ...
... Functional cloning - xl forex com, forex news with analysis, forex minor trend within major trend, forex reserves per country
3v3 Singles 2 day DQ 2 subs 2 recoveries/5 chills ASB Arena Switch=OK All Abilities Items=Sure, Wynaut In the left corner, we have...
KIAA1543兔多克隆抗体(ab122864)可与人样本反应并经IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Development of a method for mRNA differential display in filamentous fungi: comparison of mRNA differential display reverse transcription polymerase chain reaction and cDNA amplified fragment length polymorphism in Leptosphaeria maculans
Definition of differential display in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is differential display? Meaning of differential display as a finance term. What does differential display mean in finance?
TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal ),RT-PCR,PCR, RT-PCR, qPCR, qRT-PCR,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOv
First-strand cDNA Synthesis System for Quantitative RT-PCR has been designed for the highest efficiency conversion of RNA to cDNA and is fully optimized for quantitative real-time PCR applications.
OriGene offers comprehensive product solutions for studying human protein kinases. Our functional kinome cDNA collection was featured in a Cell publication in 2008.
Purified Human Fetal Brain Cytoplasmic Lysate from Creative Biomart. Human Fetal Brain Cytoplasmic Lysate can be used for research.
QIAGENs single cell NGS solutions combine industry-leading performance, robustness, and sensitivity to deliver exceptional data quality. Our complete solutions cover the entire single cell NGS workflow, starting from single cells and ending with PCR-free NGS libraries, all with a single kit. Whether you are analyzing isolated single cells or complex tissues for sequence and structural variants, conducting PGD research, analyzing gene expression and detecting rare transcripts, or generating de novo genomes from a single bacterium, QIAseqs single cell NGS kits provide you with unprecedented sensitivity and accuracy at competitive all-in pricing that allows you to analyze more cells. Discover QIAseq solutions for single cell NGS today ...
This book offers practical advice to researchers interested in using the technique of RT-PCR differential display to help them understand the function of individual genes in specific tissues or cells of an organism, either as part of normal development and aging, or in disease processes.
With enhanced promotor, GSK3B cDNA ORF Clone, Mouse in pCMV3-N-OFPSpark is expression-ready, and confirmed by full-length sequence & expression validation
Cloning of Eg7 cDNA. (A) The open box represents the coding region (1,360 amino acids). Eg7.1-Eg7.4 correspond to partial cDNAs obtained by screening a Xe
View mouse BC052040 Chr2:115581716-115778768 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Basics of microarrays Petter Mostad 2003 Why microarrays? Microarrays work by hybridizing strands of DNA in a sample against complementary DNA in spots on a chip. Expression analysis measure relative amounts
গল্পের শুরু ১৮১৫ সালে লস এঞ্জেলসে এক মুভি Stuntman এর ভয়াবহ পাগলাটে এক লাফ দিয়ে। রয় ওয়াকার নামে এই ভদ্রলোক Stuntman হিসেবে তার জীবনের প্রথম মুভিতে রেল ব্রিজ হতে এক অকল্পনীয় লাফ দিয়ে নিজেকে প্রায় পঙ্গু করে ফেলে হাসপাতালে ভর্তি হন। আর কমলালেবুর বাগানে কমলা তুলতে গিয়ে হাত ভেঙ্গে ফেলা আলেকজান্দ্রিয়া নামে এক ছোট্ট পরীও ভর্তি হয় ঐ হাসপাতালে। এক সকালে সে তার প্রিয় নার্সকে চরকা কাটা এক ...
Huge conductance Ca2+-activated K+ (BKCa) stations encoded with the gene (also called appearance using media supplemented using the chromogenic substrate 5-bromo-4-chloro-3-indolyl-Top 10 cells (Invitrogen, Carlsbad, CA) and cDNAs encoding putative interacting protein were sequenced. chick ciliary ganglion (21, 22) aswell such as mouse podocytes from the renal glomerulus (24). We lately proposed which THZ1 manufacturer the Slo1 COOH-terminals may work as PDZ domains binding motifs that are likely involved in regulating steady-state surface area appearance of BKCa stations (22). To explore this simple idea, we performed fungus two-hybrid screens of the embryonic chick ciliary ganglion cDNA collection using baits produced from all three COOH-terminal Slo1 variants. An obvious interaction using a cDNA collection clone encoding MAGI-1 surfaced in the Slo1VEDEC bait. A schematic diagram from the domains framework of MAGI-1 is THZ1 manufacturer normally proven in Fig. 1and are extended views from the ...
A strategy was developed to generate expressed sequence tags (ESTs) from human pancreatic islet gene products using differential display of mRNA. Screening of over 2,000 cDNA amplification products identified 42 cDNAs that were preferentially expressed in pancreatic islets relative to exocrine tissue. Public database analysis showed that 29 (69%) corresponded to novel genes, in contrast with only 66 of 250 (26.4%) cDNA clones randomly selected from a human islet library. Reverse transcription-polymerase chain reaction (RTPCR) and/or Northern analysis of RNA from multiple tissues confirmed that expression was enhanced in human islet cell RNA for 11 of 15 tested cDNAs. Sequencetagged sites developed from 19 islet cDNAs were used to map these genes to human chromosomes using a combination of monochromosomal somatic-cell hybrids, genome-wide radiation hybrids, and mega-yeast artificial chromosome analysis. These results indicate that this PCR-based cDNA selection strategy yields information on a ...
Abstract Expressed sequence tags (ESTs) are complementary deoxyribonucleic acid (cDNA) fragments, which are reverse transcribed from mature ribonucleic acid (mRNA), a direct gene transcript. ESTs are a readily rich information source of complete expressed gene sequences. They reveal the type and number of genes being expressed in an organism. Joining ESTs into complete gene sequences is computationally expensive because they are numerous, erroneous, redundant and mixed up. ESTs that originate from the same gene are grouped together. This enables efficient consensus sequences generation, which reveals underlying gene sequences and their possible alternative splicings. EST clustering enables efficient discovery of expressed genes based on which several fields rely such as: disease diagnostics, drug discovery, genetic engineering, alternative splicing and many others. Most clustering algorithms developed so far are quadratic and their running time is prohibitively high. A tree-structured index ...
Given the great publicity of DNA microarrays techniques in recent years, many researchers have put their blind trust into this methodology, only to be overwhelmed by the confusing data generated. Knowing the major differences between differential display and DNA microarrays, both in theory and in practice, may help you to find the genes of real interest and save your valuable resources and effort.. Both differential display (DD) and microarrays are conceptually simple, however, the two methods are principally different. The fundamental difference is that differential display visualizes the mRNAs in subsets directly without any data normalization after their amplification and labeling by either isotopes or fluorescent dyes. In contrast, DNA microarrays visualize the mRNAs indirectly after the hybridization of extremely complex mRNA probes as first-strand cDNAs with fluorescent labels to a set of cDNA templates spotted on a glass surface. In fact, a cDNA probe used for microarray can be so complex ...
AccuPower®CycleScript RT PreMix (dT20 & dN12 & dN6)는 순환 온도 역전사 반응(Cyclic Temperature Reverse Transcription, CTRT, 특허등록 10-0984483)에 적합하도록 최적화된 제품입니다. 세계적으로 인정 받은 순환 온도 역전사 반응은 cDNA 합성 효율을 증가 시킬 뿐 아니라 Full-length cDNA 합성에 효과적입니다. , CycleScript reverse transcriptase, dNTPs, reaction buffer 및 primer 등 순환 온도 역전사 반응 또는 단일 온도 역전사 반응 (Fixed Temperature Reverse Transcription, FTRT) 에 필요한 구성 성분을 혼합하여 1회 분량씩 건조 시킨 제품입니다. 실험자는 Template RNA 준비만으로 cDNA 합성을 할 수 손쉽게 할 수 있도록 고안된 제품입니다. 본 제품은 기존의 역전사 반응 온도인 42℃ 뿐만 아니라 높은 온도인 55℃ 에서도 RT 반응을 할 수 있어 단일 온도의 역전사 반응과 함께 순환 온도 역전사 반응을 할 수 있도록 개발 ...
Complementary DNA definition: a form of DNA artificially synthesized from a messenger RNA template and used in genetic... | Meaning, pronunciation, translations and examples
Definition of complementary DNA. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGAGCTCCA CCGCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGGCACGAG AAAACCTTCA CTACTCTTGA CCCTGCGTCC CTAGCTTGGC TGACAGAGGA GCCAGGGCCA ACAGAGGTCA CACGCACATC CCAAAGCCCT CGCTCTCCAG ATTCCAGTCA GAGTTCTATG GCCCAGGAGG AAGAGGAGGA AGAGCAAGGA AGAACTAGGA AACGGAAACA GAGTGGTCAG TGCCCAGCCC ...
Cloning Mitochondrial Targeting Sequence - posted in Molecular Cloning: Dear All,Im actually having problems to clone a 95 bp fragment into a 7kbp vector.I ordered the dsDNA 95 bp oligo, containing a mitochondrial targeting sequence (MTS) from microsynth.I included the restriction sites for EcoRI and BamHI, with additional 6 bp facing outwards.Sequence:tctgcagaattcATGTCCGTCCTGACGCCGCTGCTGCTGCGGGGCTTGACAGGCTCGGCCCGGCGGCTCCCAGTGCCGCGCGCCAAGggatccaccatgUpper letters: MTSUnderlined: Rest...
In contrast, the mAb PC61, that was previously reported to recognize a determinant distal to the IL-2 binding site on the mouse p55 subunit of IL-2R and to dissociate IL-2 from the high-affinity IL-2R complex by altering the conformation of the p55 molecule itself, inhibited the low-affinity binding and cross-linking of IL-2 to the p55 molecule on mouse p55 cDNA-transfected cells ...
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We have isolated a full-length cDNA clone for a novel 29 kDa protein that is highly expressed in rat enamel cells. The clone… Expand ...
GENEWIZ provides cDNA verification to confirm your cell line development efforts through verification of recombinant and endogenous targets at the cDNA level.
No DNA after midiprep - posted in Molecular Cloning: Hi all, So, this is driving me mad and I hope that somebody can help me. Basically, I have NO DNA after my midiprep. This is what happens: I checked several minipreps, which contained the construct I desired (good yield, perfect restriction). I use a bit of the miniprep culture (stored o/n at 4 degrees) to inoculate a midiprep (100 ml), do a midiprep with the Machery-Nagel kit and NO pellet. The midiprep culture was very cloudy, so pl...
... , also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
With enhanced promotor, IFT22 cDNA ORF Clone, Cynomolgus in pCMV3-N-FLAG is expression-ready, and confirmed by full-length sequence & expression validation
With enhanced promotor, CD7 cDNA ORF Clone, Human in pCMV3-C-FLAG is expression-ready, and confirmed by full-length sequence & expression validation
With enhanced promotor, SF3B4 cDNA ORF Clone, Human in pCMV3-N-Myc is expression-ready, and confirmed by full-length sequence & expression validation
With enhanced promotor, EVI2A cDNA ORF Clone, Mouse in pCMV3-SP-N-His is expression-ready, and confirmed by full-length sequence & expression validation
This resource is called as RIKEN Arabidopsis full-length cDNA clones (RAFL clones) that has been developed by RIKEN Genomic Sciences Center (GSC) in Yokohama, Japan. The clones were deposited from Dr. Kazuo Shinozaki who is a director of RIKEN Plant Science Center (PSC) and a former group director of Plant Functional Genomics Research Group in RIKEN GSC. The RIKEN GSC, SSP (Salk/Stanford/PGEC) consortium and National Institute of Genetics (NIG) have characterized full-length sequence of approx. 18,000 RAFL clones with the supports from NSF 2010 Project and National BioResource Project. By the SSP consortium, the RAFL clones are called as R clone and distinguished from U clone that harbors ORF sequence of R clone in pUNI vector.. ...
(KudoZ) English to Polish translation of complete open reading frame of a cDNA molecule: otwarta ramka odczytu (cząsteczki cDNA) [Medical].
770 full-length cDNAs. Analysis of the mouse transcriptome based on functional annotation of 60s profile, publications, research topics, and co-authors
... ,The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well Master Plate, where each well contains plasmid DNA from 5,000 clones. Having identified the posi,biological,biology supply,biology supplies,biology product
Construct high quality full length cDNA libraries using SuperScript® reverse transcriptase combined with Gateway® cloning for uncut libraries (no use of restriction digestion). Or use standard restriction enzyme cloning.
... ,The Rapid-Screen cDNA Library Panels were designed for full-length gene cloning and require just three sets of PCRs to identify the desired cDNA clone. The first PCR analysis is performed in a 96-well Master Plate, where each well contains plasmid DNA from 5,000 clones. Having identified the posi,biological,biology supply,biology supplies,biology product
FABP7, 0.1 mg. FABP7 was initially isolated from a human fetal brain cDNA library and whose mRNA was expressed in adult brain and muscle tissues at low levels.
The AccuPower® RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix of M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal concentrations. Simply add template RNA, primers and water to start your reaction ...
Creative Biolabs provides CellRapeutics™ RevertAid First Strand cDNA Synthesis Kit product for Biopharmaceutical research,preclinical and clinical trials.
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Complement (complementary DNA) -- In genetics, complementary DNA (cDNA) is DNA synthesized from a mature mRNA template in a reaction catalyzed by the enzyme reverse transcriptase. cDNA is often used to clone eukaryotic genes in prokaryotes. cDNA is also produced by retroviruses (such. fact lexicon with terms going straight to the point. Facts are sorted by community importance and you can build your personalized lexicon
BRITAINS first cloned dog made her first TV appearance today, as mini Winnie the dachshund took to the This Morning sofa with her genetic twin.
With enhanced promotor, HDGF cDNA ORF Clone, Cynomolgus in pCMV3-C-Myc is expression-ready, and confirmed by full-length sequence & expression validation
With enhanced promotor, PSMD7 cDNA ORF Clone, Human in pCMV3-N-FLAG is expression-ready, and confirmed by full-length sequence & expression validation
With enhanced promotor, CD63 cDNA ORF Clone, Rhesus in pCMV3-SP-N-Myc is expression-ready, and confirmed by full-length sequence & expression validation
With enhanced promotor, CD5 cDNA ORF Clone, Human in pLV-C-GFPSpark is expression-ready, and confirmed by full-length sequence & expression validation
With enhanced promotor, TIM-1 / KIM-1 cDNA ORF Clone, Canine in pCMV3-SP-N-Myc is expression-ready, and confirmed by full-length sequence & expression validation
TNF (TNFA) cloned gene : ORF from ATG to Stop, in pUNO1 expression plasmid selectable in E.coli and mammalian cells. Fully sequenced.
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
Pros and Cons of Cloning The process of cloning has remained one of the most controversial topics as debates continue about the pros and cons of Cloning.
KIAA1370兔多克隆抗体(ab122551)可与人样本反应并经IHC, ICC/IF实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Last week the doctor started me on a trial of Valium. It hadnt worked when I tried with in the past, but things can change, so I agreed to try it again. Valium lasts longer than Ativan, so it is preferable in that sense. On a tiny dose of Valium (only 2 mg) I was feeling like a zombie. I didnt want to eat, I didnt want to drink much, I didnt care to do much but lay there. I was exhausted but couldnt sleep. Adam tried calling the doctor but couldnt get through ...
The full-length ORF clone contains only the coding sequence of the full-length protein, while the other full-length cDNA clones contain some untranslated sequences, such as the 5side or 3′ side non translation. It is well known that these untranslated sequences may have a negative effect on the transcription and translation processes of the encoded proteins in the host.. If it is the ORF expressed clone, it can be transfected into cells and expressed in cells.. In the general situation, the carrier of cDNA clone is not the expression vector, so it can not be directly used for transfection of cells.. The difference between ORF, cDNA and CDS:. 1.what is a full-length ORF clone?. A full-length ORF clone is a plasmid inserted into a DNA fragment encoding a full-length protein. The inserted DNA fragment only contains sequence incoding a full-length protein, and does not contain the untranslated region of 5 or 3 end (UTRs) or intron.. 2.why should the full length ORF cDNA clones are used instead ...
Yeast two-hybrid system. The C-terminal regions of mGluRs (ct-mGluRs) and the GABAB2 subunit of GABA receptors were amplified by PCR from plasmids encoding the full-length rat receptors or by reverse transcriptase (RT)-mediated PCR from rat brain total RNA. The amplified fragments were subcloned in frame into a bait plasmid, pAS2-1 (Clontech, Palo Alto, CA). The appropriate integrity of inserts was verified by DNA sequencing. In yeast two-hybrid screening, an adult rat brain cDNA library fused to the GAL4 activation domain (MATCHMAKER GAL4 cDNA library; Clontech) was cotransformed into yeast Y190 (Clontech) with the bait plasmid containing the ct-mGluR2 sequence. Colony selection and β-galactosidase reporter gene assay were performed as described previously (Dev et al., 1999). Yeast two-hybrid screening of tamalin-binding proteins from a rat brain cDNA library was conducted with use of the bait plasmid containing the full-length rat tamalin sequence.. cDNA cloning and sequence analysis. Four ...
TY - ABST. T1 - Virulence in pigs of vPader10 rescued from an infectious cDNA clone of the CSFV strain Paderborn. AU - Friis,Martin Barfred. AU - Nielsen,Jens. AU - Uttenthal,Åse. AU - Belsham,Graham. AU - Rasmussen,Thomas Bruun. PY - 2012. Y1 - 2012. N2 - The BAC clone, pBeloPader10, contains a complete cDNA of the CSFV strain Paderborn. Virus, named vPader10, was rescued from this construct by electroporation of RNA transcripts into porcine PK15 cells. To further study the characteristics of vPader10, we evaluated the virulence of this virus in vivo in pigs. An animal experiment was performed where three pigs were inoculated with vPader10 and housed in-contact with two non-inoculated pigs for 5 weeks. Following inoculation with vPader10, two out of three pigs displayed severe clinical signs of CSF from PID 14 that progressed until death of the pigs at PID 21 and PID 22, respectively. High fever (,41ºC) was observed in these pigs from PID 14 and remained at a high level until day of death. ...
Our scientists perform extensive and careful designing of the desired sequence for codon optimization, randomization, and mutation, insertion of reporter gene sequences or tags to facilitate subsequent detection /purification of the desired ligand, cDNA or the antibody. For cDNA phage display libraries, Rx Biosciences uses a novel protocol, which avoids restriction enzyme mediated cDNA truncation, as commonly used by other vendors for cDNA cloning. Hence our protocol enhances the chance of display of full length proteins at the surface of the phages. Rx Biosciences also constructs normalized, subtracted and full-length cDNA libraries in different phage display vectors. We pioneer in recombinant antibody library construction, screening and overexpression for large scale isolation and purification of monoclonal antibodies.. To receive a custom quote, please contact us. We look forward to hearing from you!. Receive a Quote ...
TY - JOUR. T1 - Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma. AU - DeYoung, Katherine L.. AU - Ray, Michael E.. AU - Su, Yan A.. AU - Anzick, Sarah L.. AU - Johnstone, Ricky W.. AU - Trapani, Joseph A.. AU - Meltzer, Paul S.. AU - Trent, Jeffrey M.. PY - 1997. Y1 - 1997. N2 - Chromosome 6-mediated suppression of tumorigenicity in malignant melanoma cell lines provides a model system to identify genes associated with the reversion of the tumorigenic phenotype. Using subtractive cDNA selection, we recently identified a series of novel genes which are differentially expressed in association with chromosome 6-mediated suppression. We now report the molecular characterization of a novel gene termed AIM2 for (Absent In Melanoma), which represents a 1485 bp cDNA. An open reading frame of 1032 base pairs, corresponding to 344 amino acid residues, is predicted. The predicted protein shares a conserved sequence ...
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GenEZ™ ORF cDNA clones makes it easy to order customized expression-ready ORF clones from the worlds largest commercial ORF clone database. You can Browse ORF cDNA clones by species Homo sapiens, letter l, page 1
GenEZ™ ORF cDNA clones makes it easy to order customized expression-ready ORF clones from the worlds largest commercial ORF clone database. You can Browse ORF cDNA clones by species Homo sapiens, page 1
Once proved to be differentially expressed, a cDNA fragment is sequenced to search for homology with other cloned genes and/ or putative gene functions. The sequence of a deoxyribonucleic acid...
Blog on FXYD6 cdna clone product: The FXYD6 fxyd6 (Catalog #MBS1276018) is a cDNA Clone and is intended for research purposes only. The pro...
Blog on ALB cdna clone product: The ALB alb (Catalog #MBS1278192) is a cDNA Clone and is intended for research purposes only. The product...
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in Swine - Suppression Subtractive Hybridization (SSH);Gene Expression;Cumulus Cells, Porcine;
An Mr 110,000 antigen was initially described in human gastric carcinoma cells by its cross-reactivity with anti-carcinoembryonic antigen (CEA) monoclonal antibodies, as well as the ability of γ-interferon to increase its level of expression. We describe the molecular cloning and sequence analyses of overlapping clones that constitute a full-length complementary DNA that encodes for the entire Mr 110,000 molecule. The 1.5-kilobase message encodes for a 407-amino acid polypeptide whose structural analysis was consistent with an integral membrane glycoprotein. In particular, the extracellular domain was rich in serine and threonine residues at which carbohydrate substitution is likely through O- and N-linked glycosylation. This would explain the higher molecular weight of the antigen whose polypeptide backbone is approximately Mr 42,000. Further computer-aided sequence analyses revealed no significant homology with any member of the CEA gene family. The cross-reactivity with anti-CEA monoclonal ...
A limiting factor of cDNA microarray technology is the need for a substantial amount of RNA per labeling reaction. Thus, 20-200 micro-grams total RNA or 0.5-2 micro-grams poly (A) RNA is typically required for monitoring gene expression. In addition, gene expression profiles from large, heterogeneous cell populations provide complex patterns from which biological data for the target cells may be difficult to extract. In this study, we chose to investigate a widely used mRNA amplification protocol that allows gene expression studies to be performed on samples with limited starting material. We present a quantitative study of the variation and noise present in our data set obtained from experiments with either amplified or non-amplified material. Using analysis of variance (ANOVA) and multiple hypothesis testing, we estimated the impact of amplification on the preservation of gene expression ratios. Both methods showed that the gene expression ratios were not completely preserved between amplified and non
Production of complementary DNA representing RNA viral sequences by recombinant DNA methods and uses therefor, retrieved 2014- ... Racaniello, Vincent R.; David Baltimore (1981-11-20). "Cloned Poliovirus Complementary DNA is Infectious in Mammalian Cells". ... As a post-doctoral fellow in David Baltimore's laboratory at MIT (1979-1982), Racaniello used recombinant DNA technology to ...
"Isolation and sequence of complementary DNA encoding human extracellular superoxide dismutase". Proceedings of the National ...
... and reverse transcriptase synthesises a complementary strand of DNA to form a double helix DNA structure ("cDNA"). The cDNA is ... The resulting DNA can be merged with the DNA genome of the host cell. The main enzyme responsible for synthesis of DNA from an ... Both DNA and RNA are nucleic acids, which use base pairs of nucleotides as a complementary language. During transcription, a ... In the case of HIV, reverse transcriptase is responsible for synthesizing a complementary DNA strand (cDNA) to the viral RNA ...
For complementary DNA generated from RNA though reverse transcription, see complementary DNA. ... Flies from Strain 1 have complementary mutations to flies from Strain 2 because when they are crossed the offspring are able to ... Meiosis is proposed to be adaptive because it facilitates recombinational repair of DNA damages that are otherwise difficult to ... and provided the foundation for the study of such fundamental processes as DNA replication and repair, and how molecular ...
DNA. The name "satellite" DNA refers to the early observation that centrifugation of genomic DNA in a test tube separates a ... The complementary sequences to two neighboring microsatellites are used as PCR primers; the variable region between them gets ... DNA refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA ... A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 1-6 or more base pairs) are ...
... is complementary to the viral mRNA. The viral DNA is found in the nucleus soon after infection of the cell. The partially ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... The genome of HBV is made of circular DNA, but it is unusual because the DNA is not fully double-stranded. One end of the full ... The nucleocapsid encloses the viral DNA and a DNA polymerase that has reverse transcriptase activity.[37] The outer envelope ...
"Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor". FEBS Letters. 317 (1-2): ...
It then produces an RNA chain, which is complementary to the template DNA strand. The process of adding nucleotides to the RNA ... DNA polymerase. DNA-directed DNA polymerase. I/A γ. θ. ν. T7. Taq. II/B α. δ. ε. ζ. Pfu. III/C. IV/X β. λ. μ. TDT. V/Y η. ι. κ ... Thermodynamically the stress accumulates from the DNA-unwinding and DNA-compaction activities. Once the DNA-RNA heteroduplex is ... Each RNAP is transcribing an RNA strand, which can be seen branching off from the DNA. "Begin" indicates the 3' end of the DNA ...
Rudner R, Karkas JD, Chargaff E (1968). Separation of B. subtilis DNA into complementary strands. III. Direct Analysis. Proc ... Eğer yeterince uzun DNA dizilerine bakılırsa her bir DNA iplikçiği %A ~ %T ve %G ~ %C kuralının olduğunu bulunmuştur.[10] ... Lobry JR (1996). "Asymmetric substitution patterns in the two DNA strands of bacteria". Mol. Biol. Evol. 13 (5): 660-665. PMID ... Chargaff'ın kuralları, James Watson ve Francis Crick'ın önerdiği üç boyutlu DNA yapısının doğruluğunu destekleyici bir bilgi ...
"β-Carboline alkaloids bind DNA". Journal of Photochemistry and Photobiology B: Biology. 100: 84-91. doi:10.1016/j.jphotobiol. ... Complementary Therapies. 7 (3): 138-143. doi:10.1089/10762800151125056. McKenna, Callaway, & Grb. "Scientific Investigation of ... bind with DNA and also exhibit anti-tumor properties. Harmine has been shown to bind one hundred times more effectively than ...
Wang LH, Ohashi K, Wu KK (1991). „Isolation of partial complementary DNA encoding human thromboxane synthase". Biochem. Biophys ... and interaction of transcriptional regulatory factor with its DNA element in the 5' flanking region". Biochim. Biophys. Acta. ...
Nucleotide sequences derived from complementary DNA". primary. The Journal of Biological Chemistry. 252 (14): 5040-53. PMID ... That is, a difference can be seen in the DNA sequence, but the differences have no effect on the growth or health of the person ... A recent study of the genes ATM, BRCA1, BRCA2, CDH1, CHEK2, PALB2, and TP53 found 15,311 DNA sequence variants in only 102 ... Less than 5% of the human genome encodes proteins, and the rest is associated with non-coding RNA molecules, regulatory DNA ...
The enzyme uses the viral RNA genome as a template for the synthesis of a complementary DNA copy. Reverse transcriptase also ... Lentiviruses can integrate a significant amount of viral cDNA into the DNA of the host cell and can efficiently infect ... Integrase processes the LTR before inserting the viral genome into the host DNA. Tat acts as a trans-activator during ... Integrase binds both the viral cDNA generated by reverse transcriptase and the host DNA. ...
... cloning and characterization of complementary DNA". Science. 245 (4922): 1066-73. Bibcode:1989Sci...245.1066R. doi:10.1126/ ... "Genomic DNA sequence".. *↑ Kavic SM, Frehm EJ, Segal AS (1999). "Case studies in cholera: lessons in medical history and ...
Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase. Science. 1985 ...
"Complementary DNA sequencing: expressed sequence tags and human genome project". Science 252 (5013).. ... 1993) A Hidden Markov Model that finds genes in E. coli DNA ... Circular SV40 DNA Molecules Containing Lambda Phage Genes and ... "DNA sequencing with chain-terminating inhibitors". Proceedings of National Academy of Sciences 74 (12). ... "Nucleotide sequence of bacteriophage λ DNA". Journal of Molecular Biology 162 (4). Arquivado dende o orixinal o 02 de decembro ...
"Complementary DNA sequencing: expressed sequence tags and human genome project". Science 252 (5013).. ... 1993) A Hidden Markov Model that finds genes in E. coli DNA ... Circular SV40 DNA Molecules Containing Lambda Phage Genes and ... "DNA sequencing with chain-terminating inhibitors". Proceedings of National Academy of Sciences 74 (12). ... "Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors" (PDF). Science 236 (4803). ...
"Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of 3' non-coding regions". Journal of ...
Hanukoglu I, Tanese N, Fuchs E (Feb 1983). "Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of ... Its DNA sequence consists of seven exons that produce five known transcripts. The majority of these consist of point mutations ... ParM is an actin homologue that is coded in a plasmid and it is involved in the regulation of plasmid DNA. ParMs from different ... It also has two different DNA promoters. It has been noted that the sequences translated from this locus and from that of β- ...
Hanukoglu I, Tanese N, Fuchs E (Feb 1983). "Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of ...
Cloning and sequence analysis of the complementary DNA". Biochemistry. 26 (13): 3975-82. doi:10.1021/bi00387a035. PMID 3651428 ...
Zietkiewicz E, Makałowski W, Mitchell G, Labuda D (October 1996). "Complementary DNA for 12-kilodalton B cell growth factor: ... Kovanen PE, Harju L, Timonen T (October 1996). "Complementary DNA for 12-kilodalton B cell growth factor: misassigned". Science ... Zietkiewicz E, Makałowski W, Mitchell GA, Labuda D (August 1994). "Phylogenetic analysis of a reported complementary DNA ...
... analysis of stomach complementary DNA sequence and expression in human and rat alimentary tracts". Gastroenterology. 112 (2): ... a human tumor-associated protein with a domain homologous to carbonic anhydrase and a putative helix-loop-helix DNA binding ...
... to the next unpaired nucleotide on the template strand it is incorporated into the growing complementary strand by the DNA ... GenomeWeb DNA Electronics Licenses IP to Ion Torrent. August 2010 *^ a b c d e f g h Rusk, N. (2011). "Torrents of sequence". ... If the introduced dNTP is complementary to the leading template nucleotide, it is incorporated into the growing complementary ... a b Pollack, A., Taking DNA Sequencing to the Masses, in New York Times. 2011: New York. ...
... cloning and characterization of complementary DNA". Science. 245 (4922): 1066-73. Bibcode:1989Sci...245.1066R. PMID 2475911. ... ATP-driven conformational control in DNA double-strand break repair and the ABC-ATPase superfamily". Cell. 101 (7): 789-800. ...
1993). "Nucleotide sequence of a complementary DNA for human ST2". Biochim. Biophys. Acta. 1171 (2): 215-8. doi:10.1016/0167- ... "Nucleotide sequence of a complementary DNA for human ST2". Biochim. Biophys. Acta. 1171 (2): 215-8. doi:10.1016/0167-4781(92) ... Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788-95 ...
RNA polymerase II regulatory region sequence-specific DNA binding. • DNA binding. • sequence-specific DNA binding. • ... 1989). "Complementary homeo protein gradients in developing limb buds". Genes Dev. 3 (5): 641-50. doi:10.1101/gad.3.5.641. PMID ... regulation of transcription, DNA-templated. • negative regulation of transcription from RNA polymerase II promoter. • positive ... transcription factor activity, sequence-specific DNA binding. • ...
"Sequence and expression of human estrogen receptor complementary DNA". Science. 231 (4742): 1150-4. doi:10.1126/science.3753802 ... Teo AK, Oh HK, Ali RB, Li BF (Oct 2001). "The Modified Human DNA Repair Enzyme O6-Methylguanine-DNA Methyltransferase Is a ... "Sequence and expression of human estrogen receptor complementary DNA". Science. 231 (4742): 1150-4. doi:10.1126/science.3753802 ... Thénot S, Bonnet S, Boulahtouf A, Margeat E, Royer CA, Borgna JL, Cavaillès V (Dec 1999). "Effect of ligand and DNA binding on ...
Base pair: Two DNA bases complementary to one another (A and T or G and C) that join the complementary strands of DNA to form ... Base in DNA Base in DNA: A unit of the DNA. There are 4 bases: adenine (A), guanine (G), thymine (T), and cytosine (C). The ... The small gap left in the DNA helix is then filled in by the sequential action of DNA polymerase and DNA ligase. Abbreviated ... Base excision repair: A process of DNA repair in which an altered base is excised (removed) by a DNA glycosylase enzyme, ...
Study of mitochondrial DNA indicates that Neanderthal man made little or no contribution to the genome of modern humans. Named ... NCCAM: National Center for Complementary & Alternative Medicine.... * NCCS NCCS: National Coalition for Cancer Survivorship ...
Definition of complementary DNA. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions ... single-stranded DNA that is complementary to messenger RNA;. *DNA that has been synthesized from mRNA by the action of reverse ...
A Drosophila Complementary DNA Resource. By Gerald M. Rubin, Ling Hong, Peter Brokstein, Martha Evans-Holm, Erwin Frise, Mark ... A Drosophila Complementary DNA Resource. By Gerald M. Rubin, Ling Hong, Peter Brokstein, Martha Evans-Holm, Erwin Frise, Mark ...
Characterization and expression of the complementary DNA encoding rat histidine decarboxylase. D R Joseph, P M Sullivan, Y M ... Characterization and expression of the complementary DNA encoding rat histidine decarboxylase. D R Joseph, P M Sullivan, Y M ... Characterization and expression of the complementary DNA encoding rat histidine decarboxylase. D R Joseph, P M Sullivan, Y M ... Characterization and expression of the complementary DNA encoding rat histidine decarboxylase Message Subject (Your Name) has ...
A Drosophila Complementary DNA Resource. By Gerald M. Rubin, Ling Hong, Peter Brokstein, Martha Evans-Holm, Erwin Frise, Mark ... A Drosophila Complementary DNA Resource. By Gerald M. Rubin, Ling Hong, Peter Brokstein, Martha Evans-Holm, Erwin Frise, Mark ... Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be ... Second, we aligned the 5′ and 3′ sequences of each clone to the genomic DNA sequence (9) and discarded clones for which the two ...
Quantitative monitoring of gene expression patterns with a complementary DNA microarray.. Schena M1, Shalon D, Davis RW, Brown ... Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression ...
In genetics, complementary DNA (cDNA) is DNA synthesized from a single stranded RNA (e.g., messenger RNA (mRNA) or microRNA) ... generating its complementary DNA based on the pairing of RNA base pairs (A, U, G and C) to their DNA complements (T, A, C and G ... This synthesizes one complementary strand of DNA hybridized to the original mRNA strand. To synthesize an additional DNA strand ... Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library. When scientists ...
Sequencing and expression of complementary DNA for the general transcription factor BTF3.. Zheng XM1, Black D, Chambon P, Egly ... On the basis of sequences from peptides of BTF3, we have now cloned two complementary DNAs, one for a protein (BTF3a) with all ...
cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as ... Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. ... Complementary" by people in Harvard Catalyst Profiles by year, and whether "DNA, Complementary" was a major or minor topic of ... "DNA, Complementary" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ...
Complementary DNA is created as a result of intron-free mRNA that is why it is referred to as complementary copy of the mRNA ... Complementary DNA is basically useful in bioinformatics where it refer to an mRNA transcript sequence expressed as DNA bases ... Complementary DNA is usually used to cloned eukaryotic genes in prokaryotes to express specific proteins and is naturally ... A double stranded DNA produced from the messenger RNA synthesis in a reaction catalyzed by an enzymes reverse transcriptase. ...
Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue- ... Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue- ... Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue- ... Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue- ...
... a form of DNA artificially synthesized from a messenger RNA template and used in genetic... , Meaning, pronunciation, ... Trends of complementary DNA. Used Rarely. complementary DNA is in the lower 50% of commonly used words in the Collins ... a form of DNA artificially synthesized from a messenger RNA template and used in genetic engineering to produce gene clones ...
Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases. Peter ... Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases ... Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases ... Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases ...
Cloning of a complementary DNA coding for the 100-kD antigenic protein of the PM-Scl autoantigen.. ... Cloning of a complementary DNA coding for the 100-kD antigenic protein of the PM-Scl autoantigen.. ...
Application of Complementary DNA Microarray Technology to Carcinogen Identification, Toxicology, and Drug Safety Evaluation. ... Schena M., Shalon D., Davis R. W., Brown P. O. Quantitative monitoring of gene expression patterns with a complementary DNA ... Application of Complementary DNA Microarray Technology to Carcinogen Identification, Toxicology, and Drug Safety Evaluation ... Application of Complementary DNA Microarray Technology to Carcinogen Identification, Toxicology, and Drug Safety Evaluation ...
China Complementary DNA Microarrays (cDNA) Market Research Report 2018 is a market research report available at US $3400 for a ... 1.2.1 China Complementary DNA Microarrays (cDNA) Sales (K Units) Comparison by Type (2013-2025). 1.2.2 China Complementary DNA ... 1.5.1 China Complementary DNA Microarrays (cDNA) Sales (K Units) and Growth Rate (%)(2013-2025). 1.5.2 China Complementary DNA ... 1.4 China Complementary DNA Microarrays (cDNA) Market by Region. 1.4.1 China Complementary DNA Microarrays (cDNA) Market Size ( ...
A fluorescent 3,7-bis-(naphthalen-1-ylethynylated)-2′-deoxyadenosine analogue reports thymidine in complementary DNA by a large ... A fluorescent 3,7-bis-(naphthalen-1-ylethynylated)-2′-deoxyadenosine analogue reports thymidine in complementary DNA by a large ... use as a fluorescent probe for structural studies of DNAs/RNAs including the detection of single-base alterations in target DNA ...
Isolation of a complementary DNA encoding a chitinase with structural homology to a bifunctional Isozyme/chitinase METRAUX J. P ... Cloning of a Complementary DNA that Encodes an Acidic Chitinase which is Induced by Ethylene and Expression of the ... A complementary DNA encoding an ethylene-inducible acidic chitinase of azuki bean ( Vigna angularis) was isolated, and its ... Cloning of a Complementary DNA for Auxin-Induced 1-Aminocyclopropane-1-carboxylate Synthase and Differential Expression of the ...
Identification of genes modulated in rheumatoid arthritis using complementary DNA microarray analysis of lymphoblastoid B cell ... using complementary DNA (cDNA) microarray analyses on lymphoblastoid B cell lines (LCLs) derived from RA-discordant monozygotic ... "Identification of genes modulated in rheumatoid arthritis using complementary DNA microarray analysis of lymphoblastoid B cell ...
... Fey, G. H.; Lundwall ... component C3 have been determined from a cloned genomic DNA fragment and several overlapping cloned complementary DNA fragments ... component C3 have been determined from a cloned genomic DNA fragment and several overlapping cloned complementary DNA fragments ... component C3 have been determined from a cloned genomic DNA fragment and several overlapping cloned complementary DNA fragments ...
Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays. ... Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays ... For PCR of genomic DNA, DNA was extracted from samples by incubating overnight at 55°C with lysis buffer containing 10 mm Tris- ... Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays ...
... to generate structure-free DNA that is fully accessible to complementary probes. The analogs, which included bioactive bases ... Either analog could be used with nA and sT to generate DNA that was nearly structure-free. Hybridization of probes to these ... were prepared as dNTPs and evaluated as substrates for T7 and Phi29 DNA polymerases that lacked editor function. Pairing ... A straightforward enzymatic protocol for converting regular DNA into pseudo-complementary DNA could improve the performance of ...
Complementary DNA Sequence of a Low Temperature-Induced Brassica napus Gene with Homology to the Arabidopsis thaliana kin1 Gene ... Complementary DNA Sequence of a Low Temperature-Induced Brassica napus Gene with Homology to the Arabidopsis thaliana kin1 Gene ... Complementary DNA Sequence of a Low Temperature-Induced Brassica napus Gene with Homology to the Arabidopsis thaliana kin1 Gene ... Complementary DNA Sequence of a Low Temperature-Induced Brassica napus Gene with Homology to the Arabidopsis thaliana kin1 Gene ...
  • Complementary DNA is usually used to cloned eukaryotic genes in prokaryotes to express specific proteins and is naturally produced by retrovirus like HIV-1 which able to incorporate to the host genome where it creates a provirus. (biology-online.org)
  • Genes also store genetic information, but in smaller quantities than DNA molecules. (reference.com)
  • The chromosomes and genes contained within DNA are replicated during the S phase of the interphase cell cycle. (reference.com)
  • Genes are individual segments of DNA and chromosomes are structures which contain many genes packed together. (reference.com)
  • In the nucleus, the NICD interacts directly with the DNA binding protein CSL and activates the transcription of target genes such as HES [ 5 ]. (hindawi.com)
  • In contrast, PTEN overexpression upregulated protein phosphatase 2A1B, ubiquitin protease (unph), secreted phosphoprotein 1, leukocyte elastase inhibitor, nuclear factor-κB, cyclic adenosine monophosphate response element binding protein, DNA ligase 1, heat shock protein 90, and some EST genes. (ncku.edu.tw)
  • Two pairs of genes are coded by the same region of DNA using different reading frames. (todayinsci.com)
  • DNA that has been synthesized from mRNA by the action of reverse transcriptase. (drugs.com)
  • This enzyme, which naturally occurs in retroviruses, operates on a single strand of mRNA, generating its complementary DNA based on the pairing of RNA base pairs (A, U, G and C) to their DNA complements (T, A, C and G, respectively). (wikipedia.org)
  • A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA (Reverse transcriptase requires this double-stranded segment as a primer to start its operation. (wikipedia.org)
  • Reverse transcriptase is added, along with deoxynucleotide triphosphates (A, T, G, C). This synthesizes one complementary strand of DNA hybridized to the original mRNA strand. (wikipedia.org)
  • the antisense strand of such a segment then peels off and binds with its complementary mRNA. (encyclopedia.com)
  • The mRNA code will be different to the orginal DNA strand. (prezi.com)
  • 3. Each tRNAs anti-codon links to its complementary codon on the mRNA. (prezi.com)
  • Chromosomes are important because they contain DNA, the biochemical substance required for the molecular-level cellular messages known as gene expression. (reference.com)
  • Five UDP glucuronosyltransferases (UGT) were synthesized from complementary DNAs expressed in COS 7 cells and were tested for their capacities to glucuronidate a range of 2-acetylaminofluorene and benzo( a )pyrene-hydroxylated metabolites. (aacrjournals.org)
  • The types of junk DNA include introns, pseudogenes, and mobile and repetitive DNAs. (creation.com)
  • Different methods including flow cytometry, comet assay and reverse transcription-polymerase chain reaction (RT-PCR) were used to show the effects of juice exposure on the level of DNA damage and the reduction of cancerous cells. (biomedcentral.com)
  • We examined the cell proliferation effect and mechanism of C. concinna -derived cryptocaryone (CPC) on oral cancer cells in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial depolarization, and DNA damage. (biomedcentral.com)
  • Moreover, γH2AX flow cytometry showed DNA damage in CPC-treated Ca9-22 cells. (biomedcentral.com)
  • CPC-induced cell responses in terms of cell viability, apoptosis, oxidative stress, and DNA damage were rescued by N-acetylcysteine pretreatment, suggesting that oxidative stress plays an important role in CPC-induced death of oral cancer cells. (biomedcentral.com)
  • These resting cells likely integrate HIV-DNA upon activation and then contribute to HIV viremia and viral spread. (clinicaltrials.gov)
  • Within eukaryotic cells, DNA is organized into long structures called chromosomes . (wikipedia.org)
  • and expression and integration of viral DNA in animal cells. (elsevier.com)
  • Detection of virus-specific RNA in simian sarcoma-leukemia virus-infected cells in in situ hybridization to viral complementary DNA. (semanticscholar.org)
  • The technique was applied to cells infected with simian sarcoma-leukemia virus, where the virus-specific RNA was detected by hybridization to simian sarcoma-leukemia virus 3H-labeled complementary DNA. (semanticscholar.org)
  • For instance, the newt Triturus cristatus has around six times as much DNA as humans, who have about 7.5 times as much as the pufferfish Fugu rubripes . (creation.com)
  • Purported remark made at The Eagle pub (28 Feb 1953), near the Cavendish Laboratory in Cambridge, to celebrate the fact that they, Crick and Watson, had unravelled the structure of DNA. (todayinsci.com)
  • Stated by James Watson in The Double Helix: A Personal Account of the Discovery of the Structure of DNA (1968, 1998), 197. (todayinsci.com)
  • The present invention relates generally to polynucleotides and DNA and RNA probes, their preparation and their use in genetic characterization. (google.com.au)
  • Here we screened several highly destabilizing analogs of G and C for one that could be used with 2-aminoadenine (nA) and 2-thiothymine (sT) to generate structure-free DNA that is fully accessible to complementary probes. (jefferson.edu)
  • Test samples of genomic DNA may be characterized by the use of polynucleotide probes each of which is specific for an informative genetic locus. (google.com.au)
  • Such probes may be prepared by the use of probes which are capable of differentiating DNA by reference to more than one polymorphic minisatellite region or. (google.com.au)
  • In contrast, prokaryotes ( bacteria and archaea ) store their DNA only in the cytoplasm , in circular chromosomes . (wikipedia.org)
  • DNA, Complementary" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • Moreover, glucose was very selective for guanine when attached through OH1 or OH4 to the DNA. (rsc.org)
  • 11,12 In fact, we have also observed monosaccharide stacking on top of the guanine tetrad of a G-quadruplex DNA structure. (rsc.org)
  • The majority of genetic information is stored within individual DNA molecules, although it is found in other cellular locations as well. (reference.com)
  • DNA molecules are the first levels of storage for genetic material. (reference.com)
  • The shape of the active site of enzyme molecules is complementary to a specific substrate. (google.com)
  • understand the number of DNA molecules within a chromosome. (nottingham.ac.uk)
  • the free base of zebularine) and 6-methylfuranopyrimidinone (MefP), were prepared as dNTPs and evaluated as substrates for T7 and Phi29 DNA polymerases that lacked editor function. (jefferson.edu)
  • In contrast, Bst 2.0, SIII and BIOTAQ™ DNA polymerases seem to display a loop-out mechanism possibly due to the flexible glycerol linker used instead of deoxyribose. (rsc.org)
  • The Austrian biochemist Erwin Chargaff, inspired by Oswald Avery's 1944 paper on DNA as genetic material, launched a research project focused on the chemistry of nucleic acids in the 1950s. (news-medical.net)
  • In C. elegans , the nuclear-localized Agos HRDE-1 (germline) and NRDE-3 (soma) bind 2° siRNAs in the cytoplasm, move to the nucleus, and interact with complementary nascent transcripts via base pairing of their associated 2° siRNAs. (genetics.org)
  • Eukaryotic organisms ( animals , plants , fungi and protists ) store most of their DNA inside the cell nucleus as nuclear DNA , and some in the mitochondria as mitochondrial DNA or in chloroplasts as chloroplast DNA . (wikipedia.org)
  • The idea that a large portion of the genomes of eukaryotes * 4 is made up of useless evolutionary remnants comes from the problem known as the 'c-value paradox', 'c' meaning the haploid * chromosomal DNA content. (creation.com)
  • Although viruses share several features with living organisms, such as the presence of genetic material (DNA or RNA), they are not considered to be alive. (encyclopedia.com)
  • The last decade of the 20th century has seen an explosion in research into the structure and function of the DNA in genomes of a wide range of organisms. (creation.com)
  • As of April 2000, the whole genomes, or full DNA complements of over 600 organisms have been sequenced or mapped. (creation.com)
  • Junk or 'selfish' DNA is believed to be largely parasitic in nature, persisting in the genomes of higher organisms as 'evolutionary remnants' by their ability to reproduce and spread themselves, or perhaps because they have supposedly mutated into a function the cell can use. (creation.com)
  • DNA binds between subunits and along the top of the ring. (rcsb.org)
  • Metabarcoding is a rapid method of biodiversity assessment that combines two technologies: DNA based identification and high-throughput D. (blogspot.com)