Dictionaries, MedicalDictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Crowdsourcing: Social media model for enabling public involvement and recruitment in participation. Use of social media to collect feedback and recruit volunteer subjects.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.DNA, Complementary: Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.Dictionaries, ChemicalVocabulary, Controlled: A specified list of terms with a fixed and unalterable meaning, and from which a selection is made when CATALOGING; ABSTRACTING AND INDEXING; or searching BOOKS; JOURNALS AS TOPIC; and other documents. The control is intended to avoid the scattering of related subjects under different headings (SUBJECT HEADINGS). The list may be altered or extended only by the publisher or issuing agency. (From Harrod's Librarians' Glossary, 7th ed, p163)Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.Research Support, Non-U.S. Gov'tOligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Research Support, U.S. GovernmentResearch Support, American Recovery and Reinvestment ActResearch Support, N.I.H., ExtramuralRNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)High-Throughput Nucleotide Sequencing: Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc.Psychology, Experimental: The branch of psychology which seeks to learn more about the fundamental causes of behavior by studying various psychologic phenomena in controlled experimental situations.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Vitamin K 3: A synthetic naphthoquinone without the isoprenoid side chain and biological activity, but can be converted to active vitamin K2, menaquinone, after alkylation in vivo.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Phonetics: The science or study of speech sounds and their production, transmission, and reception, and their analysis, classification, and transcription. (Random House Unabridged Dictionary, 2d ed)Language: A verbal or nonverbal means of communicating ideas or feelings.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.RNA, Complementary: Synthetic transcripts of a specific DNA molecule or fragment, made by an in vitro transcription system. This cRNA can be labeled with radioactive uracil and then used as a probe. (King & Stansfield, A Dictionary of Genetics, 4th ed)Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Base Pairing: Pairing of purine and pyrimidine bases by HYDROGEN BONDING in double-stranded DNA or RNA.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Nasopharyngeal Neoplasms: Tumors or cancer of the NASOPHARYNX.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Cell Line, Tumor: A cell line derived from cultured tumor cells.Carcinoma: A malignant neoplasm made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases. It is a histological type of neoplasm but is often wrongly used as a synonym for "cancer." (From Dorland, 27th ed)Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.ReadingPlant Physiological Phenomena: The physiological processes, properties, and states characteristic of plants.Plant Leaves: Expanded structures, usually green, of vascular plants, characteristically consisting of a bladelike expansion attached to a stem, and functioning as the principal organ of photosynthesis and transpiration. (American Heritage Dictionary, 2d ed)Plant Proteins: Proteins found in plants (flowers, herbs, shrubs, trees, etc.). The concept does not include proteins found in vegetables for which VEGETABLE PROTEINS is available.Plant Development: Processes orchestrated or driven by a plethora of genes, plant hormones, and inherent biological timing mechanisms facilitated by secondary molecules, which result in the systematic transformation of plants and plant parts, from one stage of maturity to another.Plants: Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.Polycyclic Compounds: Compounds consisting of two or more fused ring structures.Hydrocarbons, Aromatic: Organic compounds containing carbon and hydrogen in the form of an unsaturated, usually hexagonal ring structure. The compounds can be single ring, or double, triple, or multiple fused rings.Polycyclic Hydrocarbons, Aromatic: A major group of unsaturated cyclic hydrocarbons containing two or more rings. The vast number of compounds of this important group, derived chiefly from petroleum and coal tar, are rather highly reactive and chemically versatile. The name is due to the strong and not unpleasant odor characteristic of most substances of this nature. (From Hawley's Condensed Chemical Dictionary, 12th ed, p96)Mineral Oil: A mixture of liquid hydrocarbons obtained from petroleum. It is used as laxative, lubricant, ointment base, and emollient.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Oligonucleotides: Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)Pharmacovigilance: The detection of long and short term side effects of conventional and traditional medicines through research, data mining, monitoring, and evaluation of healthcare information obtained from healthcare providers and patients.DNA Barcoding, Taxonomic: Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".Complementary Therapies: Therapeutic practices which are not currently considered an integral part of conventional allopathic medical practice. They may lack biomedical explanations but as they become better researched some (PHYSICAL THERAPY MODALITIES; DIET; ACUPUNCTURE) become widely accepted whereas others (humors, radium therapy) quietly fade away, yet are important historical footnotes. Therapies are termed as Complementary when used in addition to conventional treatments and as Alternative when used instead of conventional treatment.Metals, Heavy: Metals with high specific gravity, typically larger than 5. They have complex spectra, form colored salts and double salts, have a low electrode potential, are mainly amphoteric, yield weak bases and weak acids, and are oxidizing or reducing agents (From Grant & Hackh's Chemical Dictionary, 5th ed)Benchmarking: Method of measuring performance against established standards of best practice.Treatment Refusal: Patient or client refusal of or resistance to medical, psychological, or psychiatric treatment. (APA, Thesaurus of Psychological Index Terms, 8th ed.)Patient Compliance: Voluntary cooperation of the patient in following a prescribed regimen.
Regulation of body length and male tail ray pattern formation of Caenorhabditis elegans by a member of TGF-beta family. (1/28861)We have identified a new member of the TGF-beta superfamily, CET-1, from Caenorhabditis elegans, which is expressed in the ventral nerve cord and other neurons. cet-1 null mutants have shortened bodies and male tail abnormal phenotype resembling sma mutants, suggesting cet-1, sma-2, sma-3 and sma-4 share a common pathway. Overexpression experiments demonstrated that cet-1 function requires wild-type sma genes. Interestingly, CET-1 appears to affect body length in a dose-dependent manner. Heterozygotes for cet-1 displayed body lengths ranging between null mutant and wild type, and overexpression of CET-1 in wild-type worms elongated body length close to lon mutants. In male sensory ray patterning, lack of cet-1 function results in ray fusions. Epistasis analysis revealed that mab-21 lies downstream and is negatively regulated by the cet-1/sma pathway in the male tail. Our results show that cet-1 controls diverse biological processes during C. elegans development probably through different target genes. (+info)
Molecular cloning and epitope analysis of the peanut allergen Ara h 3. (2/28861)Peanut allergy is a significant IgE-mediated health problem because of the increased prevalence, potential severity, and chronicity of the reaction. Following our characterization of the two peanut allergens Ara h 1 and Ara h 2, we have isolated a cDNA clone encoding a third peanut allergen, Ara h 3. The deduced amino acid sequence of Ara h 3 shows homology to 11S seed-storage proteins. The recombinant form of this protein was expressed in a bacterial system and was recognized by serum IgE from approximately 45% of our peanut-allergic patient population. Serum IgE from these patients and overlapping, synthetic peptides were used to map the linear, IgE-binding epitopes of Ara h 3. Four epitopes, between 10 and 15 amino acids in length, were found within the primary sequence, with no obvious sequence motif shared by the peptides. One epitope is recognized by all Ara h 3-allergic patients. Mutational analysis of the epitopes revealed that single amino acid changes within these peptides could lead to a reduction or loss of IgE binding. By determining which amino acids are critical for IgE binding, it might be possible to alter the Ara h 3 cDNA to encode a protein with a reduced IgE-binding capacity. These results will enable the design of improved diagnostic and therapeutic approaches for food-hypersensitivity reactions. (+info)
ETO-2, a new member of the ETO-family of nuclear proteins. (3/28861)The t(8;21) is associated with 12-15% of acute myelogenous leukemias of the M2 subtype. The translocation results in the fusion of two genes, AML1 (CBFA2) on chromosome 21 and ETO (MTG8) on chromosome 8. AML1 encodes a DNA binding factor; the ETO protein product is less well characterized, but is thought to be a transcription factor. Here we describe the isolation and characterization of ETO-2, a murine cDNA that encodes a new member of the ETO family of proteins. ETO-2 is 75% identical to murine ETO and shares very high sequence identities over four regions of the protein with ETO (domain I-III and zinc-finger). Northern analysis identifies ETO-2 transcripts in many of the murine tissues analysed and in the developing mouse embryo. ETO-2 is also expressed in myeloid and erythroid cell lines. We confirmed the nuclear localization of ETO-2 and demonstrated that domain III and the zinc-finger region are not required for nuclear localization. We further showed that a region within ETO, containing domain II, mediates dimerization among family members. This region is conserved in the oncoprotein AML-1/ETO. The recent identification of another ETO-like protein, myeloid translocation gene-related protein 1, together with the data presented here, demonstrates that at least three ETO proteins exist with the potential to form dimers in the cell nucleus. (+info)
Control of growth and differentiation by Drosophila RasGAP, a homolog of p120 Ras-GTPase-activating protein. (4/28861)Mammalian Ras GTPase-activating protein (GAP), p120 Ras-GAP, has been implicated as both a downregulator and effector of Ras proteins, but its precise role in Ras-mediated signal transduction pathways is unclear. To begin a genetic analysis of the role of p120 Ras-GAP we identified a homolog from the fruit fly Drosophila melanogaster through its ability to complement the sterility of a Schizosaccharomyces pombe (fission yeast) gap1 mutant strain. Like its mammalian homolog, Drosophila RasGAP stimulated the intrinsic GTPase activity of normal mammalian H-Ras but not that of the oncogenic Val12 mutant. RasGAP was tyrosine phosphorylated in embryos and its Src homology 2 (SH2) domains could bind in vitro to a small number of tyrosine-phosphorylated proteins expressed at various developmental stages. Ectopic expression of RasGAP in the wing imaginal disc reduced the size of the adult wing by up to 45% and suppressed ectopic wing vein formation caused by expression of activated forms of Breathless and Heartless, two Drosophila receptor tyrosine kinases of the fibroblast growth factor receptor family. The in vivo effects of RasGAP overexpression required intact SH2 domains, indicating that intracellular localization of RasGAP through SH2-phosphotyrosine interactions is important for its activity. These results show that RasGAP can function as an inhibitor of signaling pathways mediated by Ras and receptor tyrosine kinases in vivo. Genetic interactions, however, suggested a Ras-independent role for RasGAP in the regulation of growth. The system described here should enable genetic screens to be performed to identify regulators and effectors of p120 Ras-GAP. (+info)
Mammalian staufen is a double-stranded-RNA- and tubulin-binding protein which localizes to the rough endoplasmic reticulum. (5/28861)Staufen (Stau) is a double-stranded RNA (dsRNA)-binding protein involved in mRNA transport and localization in Drosophila. To understand the molecular mechanisms of mRNA transport in mammals, we cloned human (hStau) and mouse (mStau) staufen cDNAs. In humans, four transcripts arise by differential splicing of the Stau gene and code for two proteins with different N-terminal extremities. In vitro, hStau and mStau bind dsRNA via each of two full-length dsRNA-binding domains and tubulin via a region similar to the microtubule-binding domain of MAP-1B, suggesting that Stau cross-links cytoskeletal and RNA components. Immunofluorescent double labeling of transfected mammalian cells revealed that Stau is localized to the rough endoplasmic reticulum (RER), implicating this RNA-binding protein in mRNA targeting to the RER, perhaps via a multistep process involving microtubules. These results are the first demonstration of the association of an RNA-binding protein in addition to ribosomal proteins, with the RER, implicating this class of proteins in the transport of RNA to its site of translation. (+info)
Identification and characterization of the human orthologue of yeast Pex14p. (6/28861)Pex14p is a central component of the peroxisomal protein import machinery, which has been suggested to provide the point of convergence for PTS1- and PTS2-dependent protein import in yeast cells. Here we describe the identification of a human peroxisome-associated protein (HsPex14p) which shows significant similarity to the yeast Pex14p. HsPex14p is a carbonate-resistant peroxisomal membrane protein with its C terminus exposed to the cytosol. The N terminus of the protein is not accessible to exogenously added antibodies or protease and thus might protrude into the peroxisomal lumen. HsPex14p overexpression leads to the decoration of tubular structures and mislocalization of peroxisomal catalase to the cytosol. HsPex14p binds the cytosolic receptor for the peroxisomal targeting signal 1 (PTS1), a result consistent with a function as a membrane receptor in peroxisomal protein import. Homo-oligomerization of HsPex14p or interaction of the protein with the PTS2-receptor or HsPex13p was not observed. This distinguishes the human Pex14p from its counterpart in yeast cells and thus supports recent data suggesting that not all aspects of peroxisomal protein import are conserved between yeasts and humans. The role of HsPex14p in mammalian peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible for an unrecognized complementation group of human peroxisome biogenesis disorders. (+info)
Down-regulation of RpS21, a putative translation initiation factor interacting with P40, produces viable minute imagos and larval lethality with overgrown hematopoietic organs and imaginal discs. (7/28861)Down-regulation of the Drosophila ribosomal protein S21 gene (rpS21) causes a dominant weak Minute phenotype and recessively produces massive hyperplasia of the hematopoietic organs and moderate overgrowth of the imaginal discs during larval development. Here, we show that the S21 protein (RpS21) is bound to native 40S ribosomal subunits in a salt-labile association and is absent from polysomes, indicating that it acts as a translation initiation factor rather than as a core ribosomal protein. RpS21 can interact strongly with P40, a ribosomal peripheral protein encoded by the stubarista (sta) gene. Genetic studies reveal that P40 underexpression drastically enhances imaginal disc overgrowth in rpS21-deficient larvae, whereas viable combinations between rpS21 and sta affect the morphology of bristles, antennae, and aristae. These data demonstrate a strong interaction between components of the translation machinery and showed that their underexpression impairs the control of cell proliferation in both hematopoietic organs and imaginal discs. (+info)
DEF-1, a novel Src SH3 binding protein that promotes adipogenesis in fibroblastic cell lines. (8/28861)The Src homology 3 (SH3) motif is found in numerous signal transduction proteins involved in cellular growth and differentiation. We have purified and cloned a novel protein, DEF-1 (differentiation-enhancing factor), from bovine brain by using a Src SH3 affinity column. Ectopic expression of DEF-1 in fibroblasts resulted in the differentiation of a significant fraction of the culture into adipocytes. This phenotype appears to be related to the induction of the transcription factor peroxisome proliferator-activated receptor gamma (PPARgamma), since DEF-1 NIH 3T3 cells demonstrated augmented levels of PPARgamma mRNA and, when treated with activating PPARgamma ligands, efficient induction of differentiation. Further evidence for a role for DEF-1 in adipogenesis was provided by heightened expression of DEF-1 mRNA in adipose tissue isolated from obese and diabetes mice compared to that in tissue isolated from wild-type mice. However, DEF-1 mRNA was detected in multiple tissues, suggesting that the signal transduction pathway(s) in which DEF-1 is involved is not limited to adipogenesis. These results suggest that DEF-1 is an important component of a signal transduction process that is involved in the differentiation of fibroblasts and possibly of other types of cells. (+info)
Kazusa cDNA project 2002: Of Mice and Men
Email: [email protected] We have conducted a human cDNA sequencing project for identification of unknown human transcripts in the past 8 years. A distinctive point of our project from other cDNA sequencing projects is in that we have focused our sequencing efforts on long cDNA clones (,4 kb), particularly those encoding large proteins in brain. This approach has brought us a unique cDNA resource consisting of large cDNA clones for newly identified human genes, which are known as KIAA cDNAs. The number of KIAA cDNAs has exceeded 2000 this year (the total number of the sequenced nucleotide residues, about 10 Mb) and we have made them publicly available. Because the number of genes encoding large proteins (,100 kDa) is always smaller than 10% of the total number of genes in eukaryotes, the number of human genes encoding large proteins is likely less than 3000. If this estimate is correct, we must have certainly entered endgame of the identification of human transcribed sequences expressed in the ...
British Library EThOS: Gene expression profiling in rat tissues by cDNA representational difference analysis
Gene profiling was performed using a PCR-based subtractive hybridisation method, called Representation Difference Analysis (RDA), and kidney tissue from congenic versus hypertensive controls for the SHRSPGla. The RDA method itself I have also refined using glycerol-enhanced mini-polyacrylamide gel electrophoresis to optimize the separation of differentially expressed RDA fragments. I have isolated 20 such fragments that are differentially expressed and have been able to positively identify them by BLASTA search against known sequences on Genbank. These include the cytochrome P450 4A3 gene, which has been well documented as differentially expressed between SHR and WKY kidneys, and the low affinity Na-dependent glucose transporter (SGLT2). The expression of SGLT2 mRNA has been shown to be kidney specific and I have shown that it is differentially expressed in the congenic strain of SP.WKYGla2a versus the parental SHRSPGla. Two novel genes have also been mapped onto our target chromosome 2 within ...
MouseMine: Publication Prediction of the coding sequences of mouse homologues of KIAA gene: III. the complete nucleotide...
We have conducted a human cDNA project to predict protein-coding sequences (CDSs) in large cDNAs (, 4 kb) since 1994, and the number of newly identified genes, known as KIAA genes, already exceeds 2000. The ultimate goal of this project is to clarify the physiological functions of the proteins encoded by KIAA genes. To this end, the project has recently been expanded to include isolation and characterization of mouse KIAA-counterpart genes. We herein present the entire sequences and the chromosome loci of 500 mKIAA cDNA clones and 13 novel cDNA clones that were incidentally identified during this project. The average size of the 513 cDNA sequences reached 4.3 kb and that of the deduced amino acid sequences from these cDNAs was 816 amino acid residues. By comparison of the predicted CDSs between mouse and human KIAAs, 12 mKIAA cDNA clones were assumed to be differently spliced isoforms of the human cDNA clones. The comparison of mouse and human sequences also revealed that four pairs of human ...
Differential Display Methods and Protocols
www.MOLUNA.de Differential Display Methods and Protocols  - Part I. MethodologiesnGlobal Analysis of Gene Expression by Differential Display: A Mathematical ModelnShitao Yang and Peng LiangnAutomation of Fluorescent Differential Display With Digital ReadoutnJonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, and Peng LiangnOrdered Differential DisplaynMikhail V. Matz and Ella A. MeleshkevitchnGeneCalling®: Transcript Profiling Coupled
cDNA Synthesis Kits - SMART cDNA Synthesis
cDNA Synthesis Kits - SMART cDNA Synthesis
Verification result of the assembled full-length cDNA. | Open-i
Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain...
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.. In our series of projects for accumulating sequence information on the coding sequences of unidentified human genes, we have newly determined the sequences of 100 cDNA clones from a set of size-fractionated human brain cDNA libraries, and predicted the coding sequences of the corresponding genes, named KIAA0711 to KIAA0810. These cDNA clones were selected according to their coding potentials of large proteins (50 kDa and more) in vitro. The average sizes of the inserts and corresponding open reading frames were 4.3 kb and 2.6 kb (869 amino acid residues), respectively. Sequence analyses against the public databases indicated that the predicted coding sequences of 78 genes were similar to those of known genes, 64% of which (50 genes) were categorized as proteins functionally related to cell signaling/communication, cell structure/motility and nucleic acid management. As additional information ...
Subtractive cDNA Library Construction Service - Creative Biogene
Construction and analysis of full-length and normalized cDNA libraries from citrus
EN] We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. In this regard, the availability of full-length cDNA clones facilitates functional analysis of the corresponding genes enabling manipulation of their expression and the generation of a variety of tagged versions of the native protein. The development of full-length cDNA sequences has the power to improve the quality of genome annotation, as well as provide tools for functional characterization of ...
Gentaur Molecular :GenWay \ Full-length cDNA clone CS0DI023YE22 of Placenta of Homo sapiens - human \ 10-288-22076F
MYO6 - ويكيبيديا
Nagase T، Ishikawa K، Nakajima D، Ohira M، Seki N، Miyajima N، Tanaka A، Kotani H، Nomura N، Ohara O (Apr 1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 4 (2): 141-50. PMID 9205841. doi:10.1093/dnares/4.2.141. ...
ADAMTSL1 - ويكيبيديا
Nagase T, Ishikawa K, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Aug 1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (1): 31-9. doi:10.1093/dnares/5.1.31. PMID 9628581 ...
Gene Expression Literature Detail
cdna-synthesis-and-reverse-transcriptases | NEB
Gentaur Molecular :GeneRiver \ Heart cDNA \ CDPI007
Highly specific cDNA synthesis for qPCR
Generating and Sequencing Full-length cDNAs of Novel Human Genes Within the German cDNA Consortium
P-57. Generating and Sequencing Full-length cDNAs of Novel Human Genes Within the German cDNA Consortium. Ruth E. Wellenreuther, Stefan Wiemann, Daniela Heiss, Nina Claudino, Annemarie Poustka, Department of Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Heidelberg, GERMANY. We generate human cDNA libraries that are enriched in full length clones i.e. from the translation start to the poly A tail. These libraries are used for a) systematic sequencing within the cDNA consortium of the Genome Project aiming at the identification and analysis of as many new genes as possible and b) for screening to isolate full length clones of partial genes. Libraries are created by directional cloning of cDNAs into plasmid vectors. Full-length enrichment is achieved via Clontechs SMART technology. This method is PCR-based, and in our modified strategy, we amplify and clone selective size windows of the cDNA fraction above 3 kb.Clones from the libraries generated within this project are ...
Invitrogen™ Cloned AMV First-Strand cDNA Synthesis Kit 100 reactions Invitrogen™ Cloned AMV First-Strand cDNA Synthesis Kit
Leicester Research Archive: Integrative annotation of 21,037 human genes validated by full-length cDNA clones
The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the ...
First-Strand cDNA Synthesis,EasyScript® First-Strand cDNA Synthesis SuperMix,RT-PCR,Beijing Tra
Database Description - KOME | LSDB Archive
Information about approximately 38,000 full-length cDNA clones that were completely sequenced in the Rice full-length cDNA project is shown in the database. The full-length cDNA clones were collected from various tissues treated under various stress conditions. The database contains not only information about complete nucleotide sequences and encoded amino acid sequences, but also results of homology searches against public databases, mapping information, information about patterns of alternative splicing, protein domains and transmembrane structures, and information about cellular localizations and gene functions annotated with Gene Ontology.. ...
14th International Mouse Genome Conference (2000)
4 Genome Science Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan. E-mail: [email protected] Functional annotation of DNA sequences is recognized as one of the most important processes in genome analysis. In this poster, we present an analysis of detecting functional motifs from the mouse full-length cDNA data, which are being sequenced by RIKEN. Our method detects functional motifs as commonly conserved regions between given cDNA sequences. Detection of such regions may give useful information for the functional assignment of cDNA sequences if the regions can be related with specific functions.. Our method mainly consists of the following two steps, both of which are based on the graph theory.. (1) Clustering of sequences: First, we compute all pairwise similarities of given protein sequences (which are translated from full-length cDNA sequences), and construct a linkage graph. In the graph, a vertex corresponds to a protein, and an edge is weighted by the ...
Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis
A large number of cDNA inserts were sequenced from a high-quality library of chicken bursal lymphocyte cDNAs. Comparisons to public gene databases indicate that the cDNA collection represents more than 2,000 new, full-length transcripts. This resource defines the structure and the coding potential o …
Zinc finger protein 30 homolog
HIST2H2BF - Wikipedia
Histone H2B type 2-F is a protein that in humans is encoded by the HIST2H2BF gene. GRCh38: Ensembl release 89: ENSG00000203814 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000105827 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". "Entrez Gene: HIST2H2BF histone cluster 2, H2bf". Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMC 139241 . PMID 12477932. Cheung WL, Ajiro K, Samejima K, et al. (2003). "Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase". Cell. 113 (4): 507-17. doi:10.1016/S0092-8674(03)00355-6. PMID 12757711. Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMC ...
Universal RiboClone cDNA Synthesis System | cDNA Cloning | cDNA Library
Mesoderm induction early response protein 2
BCLAF1 - Wikipedia
Bcl-2-associated transcription factor 1 is a Bcl-2 family protein in humans that is encoded by the BCLAF1 gene. This gene encodes a transcriptional repressor that interacts with several members of the BCL-2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different protein isoforms have been found for this gene. GRCh38: Ensembl release 89: ENSG00000029363 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000037608 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Nagase T, Seki N, Ishikawa K, Tanaka A, Nomura N (Nov 1996). "Prediction of the coding sequences of unidentified human genes. V. The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by analysis of cDNA clones from human cell ...
Breakthrough of antimicrobial peptides (AMP) is to a large extent based
... on testing of fractions of organic samples in bacterial development inhibition assays. goals. had been at that correct period just obtainable seeing that clones from a cDNA collection. 5 The overall idea was to create and characterize this peptide without prior direct BINA chemical or isolation synthesis. AMP Production Because of the little BINA bit of Rabbit polyclonal to PLSCR1. genuine peptide directly retrieved from isolates additional studies always rely on a technique to recover even more of the materials of interest. That is essential to exploit their setting of actions and their pharmaceutical potential. Since it converted out in addition it is among the larger challenges when learning more technical AMPs. Generally you can find three different techniques that may be used: immediate isolation of peptides from organic sources chemical substance synthesis or recombinant manifestation of peptides in transgenic ...
Identification of Vitis vinifera (-)-a-terpineol synthase by in silico screening of full-length cDNA ESTs and functional...
Restriction‐Mediated Differential Display (RMDD) - Current Protocols
x-charset gb2312,Hi, All, Does anybody knows how to access Rikens(Japan) full length Arabidopsis cDNA? There is a page maintained at http://www.gsc.riken.go.jp/Plant/database/index.html. Awfully, it does not contain a serch engine or Tools like Blast or Fasta etc.And it obviously is not a complete package. I would like to download the cDNA sequences. Thanks for your kindly inputs. Baiqing LIN --- ,/x-charset ...
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Completed - Entei's going to the Morg(zkiller)! | Smogon Forums
Development of a method for mRNA differential display in filamentous fungi: comparison of mRNA differential display reverse...
Novel patterns of gene expression in pituitary adenomas identified by complementary deoxyribonucleic acid microarrays and...
TY - JOUR. T1 - Novel patterns of gene expression in pituitary adenomas identified by complementary deoxyribonucleic acid microarrays and quantitative reverse transcription-polymerase chain reaction. AU - Evans, Chheng Orn. AU - Young, Andrew N.. AU - Brown, Milton R.. AU - Brat, Daniel J.. AU - Parks, John S.. AU - Neish, Andrew S.. AU - Oyesiku, Nelson M.. PY - 2001. Y1 - 2001. N2 - Pituitary adenomas account for approximately 10% of intracranial tumors, but little is known of the oncogenesis of these tumors. The identification of tumor-specific genes may further elucidate the pathways of tumor formation. We used complementary DNA microarrays to examine gene expression profiles in nonfunctioning, PRL, GH, and ACTH secreting adenomas, compared with normal pituitary. Microarray analysis showed that 128 of 7075 genes examined were differentially expressed. We then analyzed three genes with unique expression patterns and oncogenic importance by RT-real time quantitative PCR in 37 pituitaries. ...
Differential display financial definition of differential display
cDNA synthesis,gDNA removal,TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for
First-Strand cDNA, Synthesis for Quantitative RT-PCR, cDNA Synthesis Kit | OriGene
cDNA cloning and structure of mouse putative Ah receptor
Mouse cDNA clones for a putative Ah receptor have been isolated from a cDNA library of mRNA from Hepa-1 cells by an oligonucleotide probe produced by PCR with a pair of primers which was synthesized according to the reported N-terminal sequence of 26 amino acids. The cDNA clones encode a polypeptide …
Protein Kinase Products | OriGene
Human Fetal Brain Cytoplasmic Lysate Fetal Brain-129H - Creative BioMart
Single Cell - QIAGEN
QIAGENs single cell NGS solutions combine industry-leading performance, robustness, and sensitivity to deliver exceptional data quality. Our complete solutions cover the entire single cell NGS workflow, starting from single cells and ending with PCR-free NGS libraries, all with a single kit. Whether you are analyzing isolated single cells or complex tissues for sequence and structural variants, conducting PGD research, analyzing gene expression and detecting rare transcripts, or generating de novo genomes from a single bacterium, QIAseqs single cell NGS kits provide you with unprecedented sensitivity and accuracy at competitive all-in pricing that allows you to analyze more cells. Discover QIAseq solutions for single cell NGS today ...
Gene Expression - CDNA and ORF Clones | Transomic
Differential Display - R. A. Leslie; H. A. Robertson - Oxford University Press
GSK3B cDNA ORF Clone, Mouse, N-OFPSpark® tag | SinoBiological
Cloning of Eg7 cDNA. (A) The open box represents the | Open-i
BC052040 MGI Mouse Gene Detail - MGI:3026886 - cDNA sequence BC052040
Basics of microarrays. Petter Mostad PDF
The Fall- একটা ভাঙ্গা স্বপ্ন ও এক কল্পনার ফেরিওয়ালার গল্প…
গল্পের শুরু ১৮১৫ সালে লস এঞ্জেলসে এক মুভি Stuntman এর ভয়াবহ পাগলাটে এক লাফ দিয়ে। রয় ওয়াকার নামে এই ভদ্রলোক Stuntman হিসেবে তার জীবনের প্রথম মুভিতে রেল ব্রিজ হতে এক অকল্পনীয় লাফ দিয়ে নিজেকে প্রায় পঙ্গু করে ফেলে হাসপাতালে ভর্তি হন। আর কমলালেবুর বাগানে কমলা তুলতে গিয়ে হাত ভেঙ্গে ফেলা আলেকজান্দ্রিয়া নামে এক ছোট্ট পরীও ভর্তি হয় ঐ হাসপাতালে। এক সকালে সে তার প্রিয় নার্সকে চরকা কাটা এক ...
Search Results - AccessScience from McGraw-Hill Education
"β-Carboline alkaloids bind DNA". Journal of Photochemistry and Photobiology B: Biology. 100: 84-91. doi:10.1016/j.jphotobiol. ... Complementary Therapies. 7 (3): 138-143. doi:10.1089/10762800151125056. McKenna, Callaway, & Grb. "Scientific Investigation of ... bind with DNA and also exhibit anti-tumor properties. Harmine has been shown to bind one hundred times more effectively than ...
Production of complementary DNA representing RNA viral sequences by recombinant DNA methods and uses therefor, retrieved 2014- ... Racaniello, Vincent R.; David Baltimore (1981-11-20). "Cloned Poliovirus Complementary DNA is Infectious in Mammalian Cells". ... As a post-doctoral fellow in David Baltimore's laboratory at MIT (1979-1982), Racaniello used recombinant DNA technology to ...
Variant of uncertain significance
Nucleotide sequences derived from complementary DNA". primary. The Journal of Biological Chemistry. 252 (14): 5040-53. PMID ... That is, a difference can be seen in the DNA sequence, but the differences have no effect on the growth or health of the person ... A recent study of the genes ATM, BRCA1, BRCA2, CDH1, CHEK2, PALB2, and TP53 found 15,311 DNA sequence variants in only 102 ... Less than 5% of the human genome encodes proteins, and the rest is associated with non-coding RNA molecules, regulatory DNA ...
Serial analysis of gene expression
June 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. ... Comparison to DNA microarrays. The general goal of the technique is similar to the DNA microarray. However, SAGE sampling ... In 1979 teams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of ... The cDNA concatemers can then be isolated and sequenced using modern high-throughput DNA sequencers, and these sequences can be ...
Hanukoglu I, Tanese N, Fuchs E (Feb 1983). "Complementary DNA sequence of a human cytoplasmic actin. Interspecies divergence of ... Its DNA sequence consists of seven exons that produce five known transcripts. The majority of these consist of point mutations ... ParM is an actin homologue that is coded in a plasmid and it is involved in the regulation of plasmid DNA. ParMs from different ... It also has two different DNA promoters. It has been noted that the sequences translated from this locus and from that of β- ...
Zietkiewicz E, Makałowski W, Mitchell G, Labuda D (October 1996). "Complementary DNA for 12-kilodalton B cell growth factor: ... Kovanen PE, Harju L, Timonen T (October 1996). "Complementary DNA for 12-kilodalton B cell growth factor: misassigned". Science ... Zietkiewicz E, Makałowski W, Mitchell GA, Labuda D (August 1994). "Phylogenetic analysis of a reported complementary DNA ...
The 60kDa DNA/RNA binding protein and 52kDa T-cell regulatory protein are the best characterised antigens of anti-Ro antibodies ... The variable regions (yellow) are complementary to the dsDNA strands. These antibodies are found commonly in the sera of people ... Presence of high DNA-binding and low C3 levels have been shown to have extremely high predictive value (94%) for the diagnosis ... Anti-double stranded DNA (anti-dsDNA) antibodies are highly associated with SLE. They are a very specific marker for the ...
... and reverse transcriptase synthesises a complementary strand of DNA to form a double helix DNA structure ("cDNA"). The cDNA is ... The resulting DNA can be merged with the DNA genome of the host cell. The main enzyme responsible for synthesis of DNA from an ... Both DNA and RNA are nucleic acids, which use base pairs of nucleotides as a complementary language. During transcription, a ... In the case of HIV, reverse transcriptase is responsible for synthesizing a complementary DNA strand (cDNA) to the viral RNA ...
For complementary DNA generated from RNA though reverse transcription, see complementary DNA. ... Flies from Strain 1 have complementary mutations to flies from Strain 2 because when they are crossed the offspring are able to ... Meiosis is proposed to be adaptive because it facilitates recombinational repair of DNA damages that are otherwise difficult to ... and provided the foundation for the study of such fundamental processes as DNA replication and repair, and how molecular ...
1993). "Nucleotide sequence of a complementary DNA for human ST2". Biochim. Biophys. Acta. 1171 (2): 215-8. doi:10.1016/0167- ... "Nucleotide sequence of a complementary DNA for human ST2". Biochim. Biophys. Acta. 1171 (2): 215-8. doi:10.1016/0167-4781(92) ... Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788-95 ...
Estrogen receptor alpha
"Sequence and expression of human estrogen receptor complementary DNA". Science. 231 (4742): 1150-4. doi:10.1126/science.3753802 ... Teo AK, Oh HK, Ali RB, Li BF (Oct 2001). "The Modified Human DNA Repair Enzyme O6-Methylguanine-DNA Methyltransferase Is a ... "Sequence and expression of human estrogen receptor complementary DNA". Science. 231 (4742): 1150-4. doi:10.1126/science.3753802 ... Thénot S, Bonnet S, Boulahtouf A, Margeat E, Royer CA, Borgna JL, Cavaillès V (Dec 1999). "Effect of ligand and DNA binding on ...
DNA. The name "satellite" DNA refers to the early observation that centrifugation of genomic DNA in a test tube separates a ... The complementary sequences to two neighboring microsatellites are used as PCR primers; the variable region between them gets ... DNA refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA ... A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 1-6 or more base pairs) are ...
RNA is first copied as complementary DNA (cDNA) by a reverse transcriptase enzyme before the resultant cDNA is sequenced. The ... June 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. PMID ... Libraries of silkmoth mRNA transcripts were collected and converted to complementary DNA (cDNA) for storage using reverse ... Shalon D, Smith SJ, Brown PO (July 1996). "A DNA microarray system for analyzing complex DNA samples using two-color ...
Expressed sequence tag
Because these clones consist of DNA that is complementary to mRNA, the ESTs represent portions of expressed genes. They may be ... Jun 1991). "Complementary DNA sequencing: expressed sequence tags and human genome project". Science. 252 (5013): 1651-6. doi: ... Gene expression Complementary DNA (cDNA) transcriptomics IMAGE cDNA clones Whole genome sequencing (WGS) ESTs Factsheet. ... In 1979 teams at Harvard and Caltech extended the basic idea of making DNA copies of mRNAs in vitro to amplifying a library of ...
... is complementary to the viral mRNA. The viral DNA is found in the nucleus soon after infection of the cell. The partially ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ... The genome of HBV is made of circular DNA, but it is unusual because the DNA is not fully double-stranded. One end of the full ... The nucleocapsid encloses the viral DNA and a DNA polymerase that has reverse transcriptase activity. The outer envelope ...
Neurotensin receptor 1
It then produces an RNA chain, which is complementary to the template DNA strand. The process of adding nucleotides to the RNA ... DNA polymerase. DNA-directed DNA polymerase. I/A γ. θ. ν. T7. Taq. II/B α. δ. ε. ζ. Pfu. III/C. IV/X β. λ. μ. TDT. V/Y η. ι. κ ... Thermodynamically the stress accumulates from the DNA-unwinding and DNA-compaction activities. Once the DNA-RNA heteroduplex is ... Each RNAP is transcribing an RNA strand, which can be seen branching off from the DNA. "Begin" indicates the 3' end of the DNA ...
Chargaff kuralları - Vikipedi
Rudner R, Karkas JD, Chargaff E (1968). Separation of B. subtilis DNA into complementary strands. III. Direct Analysis. Proc ... Eğer yeterince uzun DNA dizilerine bakılırsa her bir DNA iplikçiği %A ~ %T ve %G ~ %C kuralının olduğunu bulunmuştur. ... Lobry JR (1996). "Asymmetric substitution patterns in the two DNA strands of bacteria". Mol. Biol. Evol. 13 (5): 660-665. PMID ... Chargaff'ın kuralları, James Watson ve Francis Crick'ın önerdiği üç boyutlu DNA yapısının doğruluğunu destekleyici bir bilgi ...
Group II pyridoxal-dependent decarboxylases
RNA polymerase II regulatory region sequence-specific DNA binding. • DNA binding. • sequence-specific DNA binding. • ... 1989). "Complementary homeo protein gradients in developing limb buds". Genes Dev. 3 (5): 641-50. doi:10.1101/gad.3.5.641. PMID ... regulation of transcription, DNA-templated. • negative regulation of transcription from RNA polymerase II promoter. • positive ... transcription factor activity, sequence-specific DNA binding. • ...
Fluorescence in situ hybridization
The fluorescent tag is then added and only binds to the complementary DNA of the suspected pathogen. If the pathogen is present ... In biology, a probe is a single strand of DNA or RNA that is complementary to a nucleotide sequence of interest. ... Repetitive DNA sequences must be blocked by adding short fragments of DNA to the sample. The probe is then applied to the ... Preparation and hybridization process - DNA. Scheme of the principle of the FISH Experiment to localize a gene in the ...
Bioinformática, a enciclopedia libre
"Complementary DNA sequencing: expressed sequence tags and human genome project". Science 252 (5013).. ... 1993) A Hidden Markov Model that finds genes in E. coli DNA ... Circular SV40 DNA Molecules Containing Lambda Phage Genes and ... "DNA sequencing with chain-terminating inhibitors". Proceedings of National Academy of Sciences 74 (12). ... "Nucleotide sequence of bacteriophage λ DNA". Journal of Molecular Biology 162 (4). Arquivado dende o orixinal o 02 de decembro ...
Stuart-Prower-faktor - Wikipédia
Further DNA testing showed that the transforming sequences in the two cancer cell lines were the same, and the gene was later ... Hall concluded that Rac and Rho are complementary for polymerised actin organisation. Indeed, Rho-dependent response is ... DNA from a rhabdomyosarcoma cell line and a fibrosarcoma cell line transformed a NIH/3T3 mouse fibroblast cell line. After ...
Index of HIV/AIDS-related articles
radiology - randomized trial - rebound - receptor (immunology) - recombinant - recombinant DNA - recombinant DNA technology - ... complementary and alternative therapy - complete blood count (CBC) - computed tomography scan (C-T scan) - concomitant drugs - ... DNA - Domain (biology) - dose-ranging study - dose-response relationship - double-blind study - drug resistance - drug-drug ... DNA) - Department of Health and Human Services (DHHS/HHS or DHHS) - desensitization - diabetes mellitus (DM) - diagnosis - ...
... reverse transcriptase creates a complementary strand of DNA from the retrovirus RNA and the RNA is degraded; this strand of DNA ... This DNA can be incorporated into host genome as a provirus that can be passed on to progeny cells. The retrovirus DNA is ... It has been speculated that the RNA to DNA transcription processes used by retroviruses may have first caused DNA to be used as ... All members of Group VI use virally encoded reverse transcriptase, an RNA-dependent DNA polymerase, to produce DNA from the ...
... to a specific DNA fragment on the filter membrane indicates that this fragment contains DNA sequence that is complementary to ... A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern ... This depurinates the DNA fragments, breaking the DNA into smaller pieces, thereby allowing more efficient transfer from the gel ... is then used to move the DNA from the gel onto the membrane; ion exchange interactions bind the DNA to the membrane due to the ...
Main articles: RNA and DNA. The major difference between RNA and DNA is the presence of a hydroxyl group at the 2'-position of ... An alternative - or complementary - theory of RNA origin is proposed in the PAH world hypothesis, whereby polycyclic aromatic ... This forces an RNA double helix to change from a B-DNA structure to one more closely resembling A-DNA. ... DNA and proteins seemed the dominant macromolecules in the living cell, with RNA only aiding in creating proteins from the DNA ...
எயிட்சு - தமிழ் விக்கிப்பீடியா
109.0 109.1 Power R, Gore-Felton C, Vosvick M, Israelski DM, Spiegel D (June 2002). "HIV: effectiveness of complementary and ... 2005). "Mitochondrial DNA and retroviral RNA analyses of archival oral polio vaccine (OPV CHAT) materials: evidence of macaque ... 1986). "Transactivation of the human immunodeficiency virus long terminal repeat sequences by DNA viruses". Proc. Natl. Acad. ...
Koshizaka T, Nishikimi M, Ozawa T, Yagi K (1988). "Isolation and sequence analysis of a complementary DNA encoding rat liver L- ... "Scientific Opinion on the substantiation of health claims related to vitamin C and protection of DNA, proteins and lipids from ... "Toward a phylogenetic classification of Primates based on DNA evidence complemented by fossil evidence". Molecular ...
... as they have additive and complementary effects on the DNA, since doxorubicin acts by intercalating between DNA strands, and ... DNA cleavage by bleomycin depends on oxygen and metal ions, at least in vitro. The exact mechanism of DNA strand scission is ... Bleomycin acts by induction of DNA strand breaks. Some studies suggest bleomycin also inhibits incorporation of thymidine ... An alternative hypothesis states that bleomycin may bind at specific sites in the DNA strand and induce scission by abstracting ...
SIDA, a enciclopedia libre
Mills E, Wu P, Ernst E (2005). "Complementary therapies for the treatment of HIV: in search of the evidence". Int J STD AIDS 16 ... the exploitation of host DNA repair mechanisms by retroviruses". ACS Chem Biol 1 (4): 217-26. PMID 17163676. doi:10.1021/ ... "Complementary and alternative medicine use among HIV-positive people: research synthesis and implications for HIV care". AIDS ...
RNA - Simple English Wikipedia, the free encyclopedia
DNA does not leave the nucleus for various reasons. DNA is a very long molecule, and is bound in with proteins, called histones ... In this way, we say that adenine is complementary to uracil and that guanine is complementary to cytosine. The first three ... This is done by messenger RNA (mRNA). A single strand of DNA is the blueprint for the mRNA which is transcribed from that DNA ... Retrotransposons copy themselves in two stages: first from DNA to RNA by transcription, then from RNA back to DNA by reverse ...
"Inferring Continental Ancestry of Argentineans from Autosomal, Y-Chromosomal and Mitochondrial DNA". Annals of Human Genetics. ... According to the provisional data of INDEC's Complementary Survey of Indigenous Peoples (ECPI) 2004 - 2005, 600,329 Natives ( ... The results of this study in which DNA from 320 individuals in which 9 Argentine provinces were examined showed that 56% of ... this study was unweighted and meant to be a representation of the diversity of Argentine DNA rather than a demonstration of the ...
டி. என். ஏ. - தமிழ் விக்கிப்பீடியா
DNA from the Beginning Another DNA Learning Center site on DNA, genes, and heredity from Mendel to the human genome project. ... இவ்வாறாக நிரப்பு இணைதாங்கிகளுக்கிடையே (complementary base pairs) நிகழும் மீள்தகு இடைவினையானது உயிரினங்களில் டி.என்.ஏ யின் ... டி.என்.ஏ (DNA) அல்லது தாயனை என்பது ஆக்சிசனற்ற ரைபோ கரு அமிலம் (Deoxyribonucleic acid அல்லது Deoxyribose nucleic acid - DNA) ... 2: The major structures in DNA compaction; DNA, the nucleosome, the 10nm "beads-on-a-string" fibre, the 30nm fibre and the ...
Propolisz - Wikipédia
2006) „Bioactive Constituents of Brazilian Red Propolis". Evidence-Based Complementary and Alternative Medicine 3 (2), 249-254 ... Ansorge, S. (July-August 2003). „Propolis and some of its constituents down-regulate DNA synthesis and inflammatory cytokine ... Evidence-Based Complementary and Alternative Medicine 5 (3), 313-316. o. DOI:10.1093/ecam/nem059. PMID 18830449. ... Evidence-Based Complementary and Alternative Medicine 2013, 697390. o. DOI:10.1155/2013/697390. PMID 23737843. ...
Harold E. Varmus
Brown, P. O.; Bowerman, B.; Varmus, H. E.; Bishop, J. M. (1987-05-08). "Correct integration of retroviral DNA in vitro". Cell. ... National Center for Complementary and Integrative Medicine. *National Center for Advancing Translational Sciences ... "DNA related to the transforming gene(s) of avian sarcoma viruses is present in normal avian DNA". Nature. 260 (5547): 170-173 ... Varmus's scientific work over the past four and a half decades include descriptions of the mechanisms by which retroviral DNA ...
Asma bahasa Indonesia, ensiklopedia bebas
Complementary DNA definition | Drugs.com
A Drosophila Complementary DNA Resource | Science
A MOLECULAR MECHANISM OF MITOMYCIN ACTION: LINKING OF COMPLEMENTARY DNA STRANDS | PNAS
Characterization and expression of the complementary DNA encoding rat histidine decarboxylase | PNAS
Characterization and expression of the complementary DNA encoding rat histidine decarboxylase. D R Joseph, P M Sullivan, Y M ... Characterization and expression of the complementary DNA encoding rat histidine decarboxylase. D R Joseph, P M Sullivan, Y M ... Characterization and expression of the complementary DNA encoding rat histidine decarboxylase. D R Joseph, P M Sullivan, Y M ... Characterization and expression of the complementary DNA encoding rat histidine decarboxylase Message Subject (Your Name) has ...
A Drosophila Complementary DNA Resource | Science
A Drosophila Complementary DNA Resource. By Gerald M. Rubin, Ling Hong, Peter Brokstein, Martha Evans-Holm, Erwin Frise, Mark ... A Drosophila Complementary DNA Resource. By Gerald M. Rubin, Ling Hong, Peter Brokstein, Martha Evans-Holm, Erwin Frise, Mark ... Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be ... Second, we aligned the 5′ and 3′ sequences of each clone to the genomic DNA sequence (9) and discarded clones for which the two ...
Complementary DNA - Wikipedia
In genetics, complementary DNA (cDNA) is DNA synthesized from a single stranded RNA (e.g., messenger RNA (mRNA) or microRNA) ... generating its complementary DNA based on the pairing of RNA base pairs (A, U, G and C) to their DNA complements (T, A, C and G ... This synthesizes one complementary strand of DNA hybridized to the original mRNA strand. To synthesize an additional DNA strand ... Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library. When scientists ...
Quantitative monitoring of gene expression patterns with a complementary DNA microarray. - PubMed - NCBI
Complementary DNA - Strand, Rna, Sequence, and Lock - JRank Articles
Complementary DNA (cDNA) is a copy of a region of a strand of DNA. For example, if the original DNA stand had a sequence of ATT ... Complementary deoxyribonucleic acid (DNA) is DNA in which the sequence of the constituent molecules on one strand of the double ... Complementary DNA is important naturally, in the manufacture of new copies of DNA, and has become an important experimental ... The result is two complete copies of DNA.. Complementary DNA has been exploited to develop research techniques and to produce ...
Sequencing and expression of complementary DNA for the general transcription factor BTF3. - PubMed - NCBI
DNA, Complementary | Harvard Catalyst Profiles | Harvard Catalyst
cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as ... Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. ... Complementary" by people in Harvard Catalyst Profiles by year, and whether "DNA, Complementary" was a major or minor topic of ... "DNA, Complementary" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ...
Complementary DNA - Biology-Online Dictionary | Biology-Online Dictionary
Complementary DNA is created as a result of intron-free mRNA that is why it is referred to as complementary copy of the mRNA ... Complementary DNA is basically useful in bioinformatics where it refer to an mRNA transcript sequence expressed as DNA bases ... Complementary DNA is usually used to cloned eukaryotic genes in prokaryotes to express specific proteins and is naturally ... A double stranded DNA produced from the messenger RNA synthesis in a reaction catalyzed by an enzymes reverse transcriptase. ...
Complementary DNA sequence and chromosomal mapping of a human proteoglycan-binding cell-adhesion protein (dermatopontin).
Complementary DNA definition and meaning | Collins English Dictionary
... a form of DNA artificially synthesized from a messenger RNA template and used in genetic... , Meaning, pronunciation, ... Trends of complementary DNA. Used Rarely. complementary DNA is in the lower 50% of commonly used words in the Collins ... a form of DNA artificially synthesized from a messenger RNA template and used in genetic engineering to produce gene clones ...
Molecular Cloning of Complementary DNA Encoding Maize Nitrite Reductase | Plant Physiology
Molecular Cloning of Complementary DNA Encoding Maize Nitrite Reductase. Kristine Lahners, Vance Kramer, Eduard Back, Laura ... Molecular Cloning of Complementary DNA Encoding Maize Nitrite Reductase. Kristine Lahners, Vance Kramer, Eduard Back, Laura ... Molecular Cloning of Complementary DNA Encoding Maize Nitrite Reductase Message Subject (Your Name) has sent you a message from ... Complementary DNA has been isolated that codes for maize nitrite reductase (NiR) by using the corresponding spinach gene (E ...
Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases |...
Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases. Peter ... Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases ... Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases ... Glucuronidation of Carcinogen Metabolites by Complementary DNA-expressed Uridine 5′-Diphosphate Glucuronosyltransferases ...
A Single Complementary-Sense Transcript of a Geminiviral DNA β Satellite Is Determinant of Pathogenicity
Mutagenesis of DNA β revealed that the complementary-sense open reading frame (ORF) βC1 is required for inducing disease ... Small circular single-stranded DNA satellites, termed DNAβ, have recently been found associated with some geminivirus ... The DNA β associated with Cotton leaf curl virus is responsible for symptom expression of a devastating disease in Pakistan. ... Tobacco plants transformed with a βC1 ORF under the control of the Cauliflower mosaic virus 35S promoter or with a dimeric DNA ...
Developmental Expression and Characterization of FS39, a Testis Complementary DNA Encoding an Intermediate Filament-Related...
Developmental Expression and Characterization of FS39, a Testis Complementary DNA Encoding an Intermediate Filament-Related ... a Testis Complementary DNA Encoding an Intermediate Filament-Related Protein of the Sperm Fibrous Sheath," Biology of ... a Testis Complementary DNA Encoding an Intermediate Filament-Related Protein of the Sperm Fibrous Sheath," Biology of ... Southern blot analysis showed FS39 to be a single copy gene, and hybridization to human genomic DNA suggested that a human ...
RCSB PDB - 6WID: Nucleotide incorporation intermediate into quaternary complex of human Polymerase Mu on a complementary DNA...
DNA-directed DNA/RNA polymerase mu. A. 356. Homo sapiens. Mutation(s): 0 Gene Names: POLM, polmu. EC: 188.8.131.52. ... Structural snapshots of human DNA polymerase mu engaged on a DNA double-strand break.. Kaminski, A.M., Pryor, J.M., Ramsden, D. ... Nucleotide incorporation intermediate into quaternary complex of human Polymerase Mu on a complementary DNA double-strand break ... DNA (5-D(*CP*GP*GP*CP*AP*T)-3). T. 6. synthetic construct. ... DNA (5-D(*CP*GP*TP*AP*T)-3). P. 5. synthetic construct. ...
JCI - Usage information: Cloning of a complementary DNA coding for the 100-kD antigenic protein of the PM-Scl autoantigen.
Phenotypic Analysis of Yellow Fever Virus Derived from Complementary DNA | The American Journal of Tropical Medicine and Hygiene
Application of Complementary DNA Microarray Technology to Carcinogen Identification, Toxicology, and Drug Safety Evaluation |...
Application of Complementary DNA Microarray Technology to Carcinogen Identification, Toxicology, and Drug Safety Evaluation. ... Schena M., Shalon D., Davis R. W., Brown P. O. Quantitative monitoring of gene expression patterns with a complementary DNA ... Application of Complementary DNA Microarray Technology to Carcinogen Identification, Toxicology, and Drug Safety Evaluation ... Application of Complementary DNA Microarray Technology to Carcinogen Identification, Toxicology, and Drug Safety Evaluation ...
China Complementary DNA Microarrays (cDNA) Market Research Report 2018 - RnR Market Research
China Complementary DNA Microarrays (cDNA) Market Research Report 2018 is a market research report available at US $3400 for a ... 1.2.1 China Complementary DNA Microarrays (cDNA) Sales (K Units) Comparison by Type (2013-2025). 1.2.2 China Complementary DNA ... 1.5.1 China Complementary DNA Microarrays (cDNA) Sales (K Units) and Growth Rate (%)(2013-2025). 1.5.2 China Complementary DNA ... 1.4 China Complementary DNA Microarrays (cDNA) Market by Region. 1.4.1 China Complementary DNA Microarrays (cDNA) Market Size ( ...
A fluorescent 3,7-bis-(naphthalen-1-ylethynylated)-2′-deoxyadenosine analogue reports thymidine in complementary DNA by a large...
A fluorescent 3,7-bis-(naphthalen-1-ylethynylated)-2′-deoxyadenosine analogue reports thymidine in complementary DNA by a large ... A fluorescent 3,7-bis-(naphthalen-1-ylethynylated)-2′-deoxyadenosine analogue reports thymidine in complementary DNA by a large ... use as a fluorescent probe for structural studies of DNAs/RNAs including the detection of single-base alterations in target DNA ...
Frontiers | Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional...
Differences in the results between DNA and RNA-based profiling demonstrated that DNA results alone can lead to the ... Differences in the results between DNA and RNA-based profiling demonstrated that DNA res... ... The complementary methodological approach, coupled with extensive bioinformatics analysis, allowed to visualize differences ... The complementary methodological approach, coupled with extensive bioinformatics analysis, allowed to visualise differences ...
A DNA strand has the base sequence of ACGT. What is the complementary RNA base pairing that would occur during transcription? ...
What is the complementary RNA base pairing that would occur during transcription? A) ACGA B) UGCA C) TCCA D) AGCT E) TTCC and ... A DNA strand has the base sequence of ACGT. ... What is the complementary strand for the DNA strand ATTCGGACTA ... The resulting new strand of mRNA has complementary base pairs to the original DNA template. DNA has four nitrogenous bases: (A ... A DNA strand has the base sequence of ACGT. What is the complementary RNA base pairing that would occur during transcription? A ...
CiNii Articles - Cloning of a Complementary DNA that Encodes an Acidic Chitinase which is Induced by Ethylene and...
Isolation of a complementary DNA encoding a chitinase with structural homology to a bifunctional Isozyme/chitinase METRAUX J. P ... Cloning of a Complementary DNA that Encodes an Acidic Chitinase which is Induced by Ethylene and Expression of the ... A complementary DNA encoding an ethylene-inducible acidic chitinase of azuki bean ( Vigna angularis) was isolated, and its ... Cloning of a Complementary DNA for Auxin-Induced 1-Aminocyclopropane-1-carboxylate Synthase and Differential Expression of the ...
Identification of genes modulated in rheumatoid arthritis using complementary DNA microarray analysis of lymphoblastoid B cell...
Identification of genes modulated in rheumatoid arthritis using complementary DNA microarray analysis of lymphoblastoid B cell ... using complementary DNA (cDNA) microarray analyses on lymphoblastoid B cell lines (LCLs) derived from RA-discordant monozygotic ... "Identification of genes modulated in rheumatoid arthritis using complementary DNA microarray analysis of lymphoblastoid B cell ...
Nucleotide sequence of complementary DNA and derived amino acid sequence of murine complement protein C3
... Fey, G. H.; Lundwall ... component C3 have been determined from a cloned genomic DNA fragment and several overlapping cloned complementary DNA fragments ... component C3 have been determined from a cloned genomic DNA fragment and several overlapping cloned complementary DNA fragments ... component C3 have been determined from a cloned genomic DNA fragment and several overlapping cloned complementary DNA fragments ...
Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays |...
Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays. ... Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays ... For PCR of genomic DNA, DNA was extracted from samples by incubating overnight at 55°C with lysis buffer containing 10 mm Tris- ... Global Gene Expression Profile of Nasopharyngeal Carcinoma by Laser Capture Microdissection and Complementary DNA Microarrays ...
"Properties of pseudo-complementary DNA substituted with weakly pairing" by Georges Lahoud, Victor Timoshchuk et al.
... to generate structure-free DNA that is fully accessible to complementary probes. The analogs, which included bioactive bases ... Either analog could be used with nA and sT to generate DNA that was nearly structure-free. Hybridization of probes to these ... were prepared as dNTPs and evaluated as substrates for T7 and Phi29 DNA polymerases that lacked editor function. Pairing ... A straightforward enzymatic protocol for converting regular DNA into pseudo-complementary DNA could improve the performance of ...
CDNAMicroarraysMoleculeSTRANDSMicroarraySequencesNucleotideGene expressionDNAsMRNASingle-strandedPolymerase chain reDeoxyribonucleicCloning of a complementary DNANucleotidesGenomicGenomeCollege of Complementary MedicineStrandProteinProteinsReplicationBase PairingCytosineSynthesisViralMolecularNucleic acidsExpressionTranscriptionCytoplasmDouble-strandedCellsHybridizationFragmentProbePolymerasesGeneticMoleculesProbesHumansHelixDuplexChromosomalNucleusComplementsOrganismsIdentification
- Collections of nonredundant, full-length complementary DNA (cDNA) clones for each of the model organisms and humans will be important resources for studies of gene structure and function. (sciencemag.org)
- In genetics, complementary DNA (cDNA) is DNA synthesized from a single stranded RNA (e.g., messenger RNA (mRNA) or microRNA) template in a reaction catalyzed by the enzyme reverse transcriptase. (wikipedia.org)
- The term cDNA is also used, typically in a bioinformatics context, to refer to an mRNA transcript's sequence, expressed as DNA bases (GCAT) rather than RNA bases (GCAU). (wikipedia.org)
- To obtain eukaryotic cDNA whose introns have been removed: A eukaryotic cell transcribes the DNA (from genes) into RNA (pre-mRNA). (wikipedia.org)
- From the hairpin loop, a DNA polymerase can then use it as a primer to transcribe a complementary sequence for the ss cDNA. (wikipedia.org)
- Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library. (wikipedia.org)
- When scientists transfer a gene from one cell into another cell in order to express the new genetic material as a protein in the recipient cell, the cDNA will be added to the recipient (rather than the entire gene), because the DNA for an entire gene may include DNA that does not code for the protein or that interrupts the coding sequence of the protein (e.g., introns). (wikipedia.org)
- With amplification of DNA sequences via polymerase chain reaction (PCR) now commonplace, one will typically conduct reverse transcription as an initial step, followed by PCR to obtain an exact sequence of cDNA for intra-cellular expression. (wikipedia.org)
- This is achieved by designing sequence-specific DNA primers that hybridize to the 5' and 3' ends of a cDNA region coding for a protein. (wikipedia.org)
- An example of this first step from viral DNA to cDNA can be seen in the HIV cycle of infection. (wikipedia.org)
- Complementary DNA (cDNA) is a copy of a region of a strand of DNA. (jrank.org)
- The cDNA will bind to the complementary site on the DNA strand. (jrank.org)
- cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. (harvard.edu)
- It is often used in gene cloning since when a gene transfer into another to express new genetic material as protein in a particular cell this cDNA will be added to that particular cell rather than the entire gene because some of DNA in the whole gene interrupts the coding sequence of the protein thus cDNA regularly obtained as expressed sequence tags. (biology-online.org)
- The global Complementary DNA Microarrays (cDNA) market is valued at XX million USD in 2017 and is expected to reach XX million USD by the end of 2025, growing at a CAGR of XX% between 2017 and 2025. (rnrmarketresearch.com)
- This report studies the Complementary DNA Microarrays (cDNA) development status and future trend in China, focuses on top players in China, also splits Complementary DNA Microarrays (cDNA) by type and by applications, to fully and deeply research and reveal the market general situation and future forecast. (rnrmarketresearch.com)
- Objective To identify disease-specific gene expression profiles in patients with rheumatoid arthritis (RA), using complementary DNA (cDNA) microarray analyses on lymphoblastoid B cell lines (LCLs) derived from RA-discordant monozygotic (MZ) twins. (umich.edu)
- To transfect the expression vectors of Pdx-1 in the mammalian cells, the complementary DNA (cDNA) of Pdx-1 was conducted. (biomedcentral.com)
- To transfect the expression vector of Pdx-1 in the mammalian cells, when the complementary DNA (cDNA) of Pdx-1 was conducted, the detected sequences were different from those reported before [ 9 ] ( https://www.ncbi.nlm.nih.gov/nuccore/454391 ). (biomedcentral.com)
- cDNA at Pdx-1, 852 base pairs (bp), was cloned by polymerase chain reaction (PCR) amplification, using PrimeSTAR ® HS DNA Polymerase (Takara Bio Inc.) and KOD-Plus-Neo ® (TOKOBO. (biomedcentral.com)
- The Global Complementary DNA Microarrays (cDNA) Market 2018 report identifies the largest production and consumption region in the world, also fastest growing region for the Complementary DNA Microarrays (cDNA) market. (publicsector24.com)
- The Complementary DNA Microarrays (cDNA) report provides the past, present and future industry trends and the forecast information related to the expected sales revenue, growth, demand and supply scenario of the Complementary DNA Microarrays (cDNA) industry. (publicsector24.com)
- Furthermore, the opportunities and the threats to the development of Complementary DNA Microarrays (cDNA) market are also covered at depth in this research report. (publicsector24.com)
- Analysis of the major industry players based on their company profiles, annual revenue, sales margin, growth aspects are also covered in the Global Complementary DNA Microarrays (cDNA) Market 2018 report, which will help other Complementary DNA Microarrays (cDNA) market players in driving business insights. (publicsector24.com)
- The analysis featured in the Global Complementary DNA Microarrays (cDNA) Market 2018 report includes important factors of the Complementary DNA Microarrays (cDNA) market based on present industry situations, market demands, business strategies utilized by Complementary DNA Microarrays (cDNA) market players and their growth synopsis. (publicsector24.com)
- This report divides the Complementary DNA Microarrays (cDNA) market based on the key players, Type, Applications, and Regions. (publicsector24.com)
- The key details related to Complementary DNA Microarrays (cDNA) industry like the product definition, cost, variety of applications, demand and supply statistics are covered in this report. (publicsector24.com)
- Competitive study of the major Complementary DNA Microarrays (cDNA) players will help all the market players in analyzing the latest trends and business strategies. (publicsector24.com)
- The study of emerging Complementary DNA Microarrays (cDNA) market segments and the existing market segments will help the readers in planning the business strategies. (publicsector24.com)
- Details about the Complementary DNA Microarrays (cDNA) industry game plan, the Complementary DNA Microarrays (cDNA) industry data source, appendix, research findings and conclusion. (publicsector24.com)
- First-strand complementary DNA (cDNA) was synthesised from 1 μg total RNA using Moloney murine leukaemia virus reverse transcriptase and primer Oligo(dT) 12-18 (both InVitrogen). (ersjournals.com)
- To assess gene expression, researchers derive complementary DNA (cDNA) from cellular RNA, label the cDNA with a fluorescent marker, wash labeled cDNA over the array, and use lasers to assess how much cDNA has stuck to each probe. (marketwatch.com)
- Two receptor-specific monoclonal antibodies were used to recover the complementary DNA (cDNA) of this regulatory protein from a chicken intestinal λgt11 cDNA expression library. (elsevier.com)
- The complementary DNA was an accurate copy of the viral RNA in that 86% of 35S viral RNA hybridized with complementary (cDNA) DNA at a 1.5 to 1 cDNA-RNA molar ratio. (elsevier.com)
- To further explore the downstream genes regulated by PTEN, a high-density complementary DNA (cDNA) microarray technique was used to profile gene changes after PTEN overexpression. (ncku.edu.tw)
- Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. (nih.gov)
- To identify novel diagnostic markers that may aid in the differential diagnosis of prostate versus urothelial carcinoma, we analyzed expression patterns in prostate and bladder cancer tissues using complementary DNA microarrays. (nih.gov)
- A straightforward enzymatic protocol for converting regular DNA into pseudo-complementary DNA could improve the performance of oligonucleotide microarrays by generating readily hybridizable structure-free targets. (jefferson.edu)
- A molecule called DNA polymerase runs the length of each strand, making a complementary copy of each strand. (jrank.org)
- In the lock in key scenario, an A pairs with the U) on the other strand, and a C always pairs with a G. Complementary RNA (cRNA) is a copy of a strand of RNA that will bind to the appropriate region of the original molecule. (jrank.org)
- Each chromosome contains one DNA molecule and. (reference.com)
- What Is the Shape of a DNA Molecule? (reference.com)
- DNA ) is a molecule composed of two chains that coil around each other to form a double helix carrying genetic instructions for the development, functioning, growth and reproduction of all known organisms and many viruses . (wikipedia.org)
- The unique material properties of DNA have made it an attractive molecule for material scientists and engineers interested in micro- and nano-fabrication. (wikipedia.org)
- The bases are stacked up the ladder and the hydrophobic bonding between the bases gives the DNA molecule stability. (news-medical.net)
- In this double helix arrangement, the width of each base pair remains the same, meaning the same distance is kept between the sugar-phosphate backbones, along the length of the DNA molecule. (news-medical.net)
- The two polynucleotide strands of the DNA double helix provide a simple basis for copying the information in the molecule. (news-medical.net)
- On separation, each of the two strands serves as a template for creating an exact or identical copy of the DNA molecule. (news-medical.net)
- e) reinserting the DNA molecule into an appropriate plasmid vector. (google.es)
- Messenger RNA (mRNA) is a molecule which carries a copy of the code from the DNA, in the nucleus, to a ribosome , where the protein is assembled from amino acids. (google.com)
- Dear student, Two cases arise here, the first one is that when a single stranded DNA or RNA is tagged with a radioactive probe is allowed to hybridise with its complementary single stranded molecule with no mutation in gene will easily hybridise because they are complementary to each other, Due to the presence of radioactive probe which is attached to the gene will be detected in autoradiography. (meritnation.com)
- The two strands are described as complementary to one another. (jrank.org)
- In DNA replication , the two strands are unwound from one another. (jrank.org)
- The two new strands are complementary to one another, and so can join together in a process called annealing. (jrank.org)
- Because ribonucleic acid (RNA) is made using DNA as the blueprint, the phenomenon of complementary strands also extends to RNA. (jrank.org)
- DNA molecules have several parts, including dual backbones, strands of four chemicals that run along their spines, and bridges, or base pairs, between their opposite backbones. (reference.com)
- A DNA microchip or gene chip is a tiny chip that has many single strands of the DNA from a specific gene attached to it. (reference.com)
- Antisense nucleotides are strings of RNA or DNA that are complementary to "sense" strands of nucleotides. (encyclopedia.com)
- Antisense DNA strands can also be made (note that in the double helix, the side of the DNA that is transcribed is itself antisense). (encyclopedia.com)
- Short antisense strands of DNA can be introduced into cells, which then bind with target mRNA. (encyclopedia.com)
- One difficulty in applying this therapy is successfully delivering the antisense DNA or RNA to all target tissues (for instance, making sure the antisense strands reach infected blood cells for HIV). (encyclopedia.com)
- The two DNA strands are also known as polynucleotides as they are composed of simpler monomeric units called nucleotides . (wikipedia.org)
- The nitrogenous bases of the two separate polynucleotide strands are bound together, according to base pairing rules (A with T and C with G), with hydrogen bonds to make double-stranded DNA. (wikipedia.org)
- Both strands of double-stranded DNA store the same biological information . (wikipedia.org)
- The two strands of DNA run in opposite directions to each other and are thus antiparallel . (wikipedia.org)
- DNA consists of 2 polynucleotide chains or strands, wound around each other such that they resemble a twisted ladder. (news-medical.net)
- In the double helix DNA structure, all four bases are confined to the inside of the double helix, held in place by hydrogen (H) bonds linking complimentary bases on the two strands. (news-medical.net)
- The two DNA strands in the double helix run in opposite directions (antiparallel to each other) to help the bases in each base pair fit into the double helix. (news-medical.net)
- Exchange between Complementary Strands of DNA? (elsevier.com)
- Quantitative monitoring of gene expression patterns with a complementary DNA microarray. (nih.gov)
- The Global DNA Microarray Marketaresegmented onbasisof technique, product and service, application, end clients and geography. (marketwatch.com)
- DNA Microarray market report 2019 flow basic inside information/data and expressive information about the DNA Microarray Industry giving a by and large factual investigation dependent on market drivers, showcase restrictions and its future prospects with development patterns, different partners like speculators, CEOs, brokers, providers, SWOT examination and others. (marketwatch.com)
- DNA microarray also many other names, including DNA chip�?genome chip�?expression chip and gene array. (marketwatch.com)
- A DNA microarray consists of a predetermined assortment of nucleic acid probes attached to a surface. (marketwatch.com)
- A microarray is a tiny, bio-compatible silicon chip capable of rapid identification and precise multiplexed analysis of nucleic acids (DNA/RNA). (marketwatch.com)
- Scope of theDNA Microarray MarketReport: This report focuses on the DNA Microarray in Global market, especially in North America, Europe and Asia-Pacific, South America, Middle East and Africa. (marketwatch.com)
- Understand how sales volumes, Global share and growth of the DNA Microarray Market will occur in the next five years. (marketwatch.com)
- Read product descriptions of DNA Microarray products, along with report scopes and upcoming trends in the industry. (marketwatch.com)
- On the basis of sequences from peptides of BTF3, we have now cloned two complementary DNAs, one for a protein (BTF3a) with all the characteristics of purified BTF3, and one for a shorter protein (BTF3b) lacking the first 44 residues of BTF3a and which is transcriptionally inactive, despite its ability to bind RNA pol II. (nih.gov)
- The sequences of the complementary DNAs from the two species are 66% homologous while the deduced amino acid sequences are 86% similar when analogous amino acids are included. (plantphysiol.org)
- A high percentage of the differences in the DNA sequences is due to the extremely strong bias in the corn gene to have a G/C base in the third codon position with 559/569 codons ending in a G or C. Using a hydroponic system, maize seedlings grown in the absence of an exogenous nitrogen source were induced with nitrate or nitrite. (plantphysiol.org)
- Thus, 3n7nz A ( 1 ) has the potential for use as a fluorescent probe for structural studies of DNAs/RNAs including the detection of single-base alterations in target DNA sequences. (rsc.org)
- The nucleotide sequences coding for murine complement component C3 have been determined from a cloned genomic DNA fragment and several overlapping cloned complementary DNA fragments. (lu.se)
- This property enables the design on a higher structural level without having to program the detailed DNA strand sequences for connecting components. (jbsdonline.com)
- The following DNA sequences illustrate pair complex or base pair in DNA. (foodshareonline.com)
- Complementary sequences will pair up in RNA just as they do in DNA. (encyclopedia.com)
- A large part of DNA (more than 98% for humans) is non-coding , meaning that these sections do not serve as patterns for protein sequences . (wikipedia.org)
- But now many of the DNA sequences formerly relegated to the junk pile have begun to obtain new respect for their role in genome structure and function, gene regulation and rapid speciation. (creation.com)
- A new creationist theory may explain how this rapid diversification came about by the changes caused by repetitive and mobile DNA sequences. (creation.com)
- With the discovery that many of these sequences seemed to have arisen from mobile DNAs which are able to reproduce themselves, the selfish or parasitic DNA hypothesis was born. (creation.com)
- Just as Plasterk was wrong about our reason for living, he is wrong about the purposes of these DNA sequences. (creation.com)
- Radioactive DNA complementary to nucleotide sequences in Moloney murine sarcoma virus (MSV) and Moloney leukemia virus (M-MuLV) complex was made by the endogenous reverse transcriptase reaction. (elsevier.com)
- Recently, AOX DNA sequences were found for the first time in four animals belonging to the phyla Mollusca, Nematoda and Chordata ( McDonald and Vanlerberghe, 2004 ). (biologists.org)
- The chemical molecules that make up DNA are known as nucleotide bases. (jrank.org)
- It maps to the DNA β at nucleotide position 186 to 563 and contains a polyadenylation signal 18 nt upstream of the stop codon. (apsnet.org)
- guanine is an example of complementary base pairing model of DNA, the rungs are A. nucleotide base pairs. (foodshareonline.com)
- These variants can now be analyzed concerning structural changes in the ternary complexes composed out of the enzyme, the DNA primer and template as well as the incoming nucleotide with the goal to rationalize the higher specificity. (uni-konstanz.de)
- The sugar present in the nucleotide is a deoxyribose, hence the name deoxyribonucleic acid (DNA). (news-medical.net)
- Frankel, AE , Neubauer, RL & Fischinger, PJ 1976, ' Fractionation of DNA nucleotide transcripts from Moloney sarcoma virus and isolation of sarcoma virus specific complementary DNA ', Journal of virology , vol. 18, no. 2, pp. 481-490. (elsevier.com)
- The ID (inhibitor of differentiation or DNA binding) helix-loop-helix proteins are important mediators of cellular differentiation and proliferation in a variety of cell types through regulation of gene expression. (isharonline.org)
- Together, Ago proteins and their associated siRNAs regulate gene expression by binding and inhibiting complementary cellular RNAs. (genetics.org)
- Chromosomes are important because they contain DNA, the biochemical substance required for the molecular-level cellular messages known as gene expression. (reference.com)
- Five UDP glucuronosyltransferases (UGT) were synthesized from complementary DNAs expressed in COS 7 cells and were tested for their capacities to glucuronidate a range of 2-acetylaminofluorene and benzo( a )pyrene-hydroxylated metabolites. (aacrjournals.org)
- The types of junk DNA include introns, pseudogenes, and mobile and repetitive DNAs. (creation.com)
- DNA that has been synthesized from mRNA by the action of reverse transcriptase. (drugs.com)
- This enzyme, which naturally occurs in retroviruses, operates on a single strand of mRNA, generating its complementary DNA based on the pairing of RNA base pairs (A, U, G and C) to their DNA complements (T, A, C and G, respectively). (wikipedia.org)
- A poly-T oligonucleotide primer is hybridized onto the poly-A tail of the mature mRNA template, or random hexamer primers can be added which contain every possible 6 base single strand of DNA and can therefore hybridize anywhere on the RNA (Reverse transcriptase requires this double-stranded segment as a primer to start its operation. (wikipedia.org)
- Reverse transcriptase is added, along with deoxynucleotide triphosphates (A, T, G, C). This synthesizes one complementary strand of DNA hybridized to the original mRNA strand. (wikipedia.org)
- The resulting new strand of mRNA has complementary base pairs to the original DNA template. (enotes.com)
- the antisense strand of such a segment then peels off and binds with its complementary mRNA. (encyclopedia.com)
- After digestion of the RNA, a single stranded DNA (ssDNA) is left and because single stranded nucleic acids are hydrophobic, it tends to loop around itself. (wikipedia.org)
- Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. (harvard.edu)
- Small circular single-stranded DNA satellites, termed DNAβ, have recently been found associated with some geminivirus infections. (apsnet.org)
Polymerase chain re1
Cloning of a complementary DNA1
- Complementarity (molecular biology) is nucleotides that forms the DNA and their complementary base pair. (foodshareonline.com)
- DNA is a long polymer made from repeating units called nucleotides , each of which is usually symbolized by a single letter: either A, T, C, or G. The structure of DNA is dynamic along its length, being capable of coiling into tight loops and other shapes. (wikipedia.org)
- This means the nucleotides in each strand of DNA are exactly complementary to that in the other strand. (news-medical.net)
- Finally, we examined DNA polymerase insertion of nucleotides in front of the saccharide unit. (rsc.org)
- Analysis of genomic DNA supports the notion that there are at least two NiR genes in maize. (plantphysiol.org)
- Southern blot analysis showed FS39 to be a single copy gene, and hybridization to human genomic DNA suggested that a human equivalent gene is present. (bioone.org)
- A major goal of genomic studies is to understand the role, if any, of the various classes of so-called 'junk' DNA. (creation.com)
- The length of helical viruses can depend on the length of the genome, the DNA or RNA within, since there are often regular structural interactions between the nucleic acids of the genome and the proteins that cover it. (encyclopedia.com)
- Complementary DNA clones to 90% of the Newcastle disease virus (NDV) genome have been produced and mapped. (microbiologyresearch.org)
College of Complementary Medicine2
- To synthesize an additional DNA strand, traditionally one would digest the RNA of the hybrid strand, using an enzyme like RNase H, or through alkali digestion method. (wikipedia.org)
- In other words, each strand acts as a blueprint to produce a complementary strand. (jrank.org)
- The association of a DNA or RNA strand to its complement is one of the basic research tools of the molecular biologist. (jrank.org)
- Northern blot analysis of RNA from the transgenic plants, using strand-specific probes, identified a single complementary-sense transcript. (apsnet.org)
- These structures demonstrate that Polμ may address complementary DSB substrates during NHEJ in a manner indistinguishable from single-strand breaks. (rcsb.org)
- Transcription is initiated when RNA polymerase connects to the DNA template strand in an area called the promoter region and starts stringing together a new complementary RNA strand in the 3' to 5' direction. (enotes.com)
- Therefore if the original DNA template strand read ACGT, the RNA strand will attach uracil to adenine so the complementary RNA strand will read UGCA. (enotes.com)
- Our study demonstrated that incorporation of the nucleoside analog PDZ-dG (3-(deoxyguanosin-N2-yl)-2-acetylamino-5-[(5-phenyloxadiazole)-2-yl]-benzene, or its analogs) into synthetic oligonucleotides significantly stabilizes the duplexes formed by these oligonucleotides and a complementary DNA strand. (jbsdonline.com)
- In addition to substantial enhancement of DNA thermal stability, the PDZ-dG containing oligonucleotides demonstrate increased mismatch discrimination, especially when the mismatched base in the opposite strand is adenine or guanine. (jbsdonline.com)
- Replication relies on complementary base pairing, to synthesize the new complementary strand. (foodshareonline.com)
- The base pairing of guanine (G) The template strand is complementary to the coding strand. (foodshareonline.com)
- During the DNA replication and repair synthesis, DNA polymerases add deoxynucleotides onto the growing end of a DNA primer strand using a singlestranded DNA as a template. (uni-konstanz.de)
- Our results indicate that βC1 protein is responsible for DNA β-induced disease symptoms. (apsnet.org)
- We identified and characterized a human orthologue of Rif1 protein, which in budding yeast interacts in vivo with the major duplex telomeric DNA binding protein Rap1p and negatively regulates telomere length. (isharonline.org)
- 5/05/2010 Multiple Choice Questions on DNA and Protein Synthesis Which of the following is an example of complementary base pairing? (foodshareonline.com)
- The pGLO plasmid is a small circular piece of DNA that contains the gene to produce green fluorescent protein in the model organism. (reference.com)
- Within eukaryotic chromosomes, chromatin proteins, such as histones , compact and organize DNA. (wikipedia.org)
- These compacting structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed. (wikipedia.org)
- DNA carries the genetic information for making proteins. (google.com)
- Such vectors allow for self-replication, inside the cells, and potentially integration in the host DNA. (wikipedia.org)
- DNA replication occurs within the nucleus of. (reference.com)
- Why is DNA replication important? (reference.com)
- Before typical cell division , these chromosomes are duplicated in the process of DNA replication , providing a complete set of chromosomes for each daughter cell. (wikipedia.org)
- What is the complementary RNA base pairing that would occur during transcription? (enotes.com)
- In C. elegans , the nuclear-localized Agos HRDE-1 (germline) and NRDE-3 (soma) bind 2° siRNAs in the cytoplasm, move to the nucleus, and interact with complementary nascent transcripts via base pairing of their associated 2° siRNAs. (genetics.org)
- What Is Complementary Base Pairing? (foodshareonline.com)
- What are the DNA base pairing rules? (foodshareonline.com)
- How are base pairing rules and complementary related? (reference.com)
- The complementary base-pairing of A with T and G with C enables optimization of energy levels within the double helix. (news-medical.net)
- DNA has four nitrogenous bases: (A) adenine, (T) thymine, (C) cytosine, and (G) guanine. (enotes.com)
- Properties of pseudo-complementary DNA substituted with weakly pairing analogs of guanine or cytosine. (jefferson.edu)
- and Gamper, Howard, "Properties of pseudo-complementary DNA substituted with weakly pairing analogs of guanine or cytosine. (jefferson.edu)
- All Watson-Crick hydrogen bonds, including the hydrogen bonds between the PDZ-dG and complementary cytosine, are fully preserved in the duplex, and all corresponding imino protons are clearly visible on the NOESY spectra in H 2 O. (jbsdonline.com)
- This permits the "real time" examination of DNA synthesis . (jrank.org)
- A double stranded DNA produced from the messenger RNA synthesis in a reaction catalyzed by an enzymes reverse transcriptase. (biology-online.org)
- Thus, the primary design goal for DNA polymerases with altered functions is high specificity in formation of Watson- Crick base pairs during DNA synthesis. (uni-konstanz.de)
- Recovery of Infectious Ebola Virus From Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity. (jrank.org)
- These resting cells likely integrate HIV-DNA upon activation and then contribute to HIV viremia and viral spread. (clinicaltrials.gov)
- and expression and integration of viral DNA in animal cells. (elsevier.com)
- Detection of virus-specific RNA in simian sarcoma-leukemia virus-infected cells in in situ hybridization to viral complementary DNA. (semanticscholar.org)
- An Introduction to Molecular Biology/DNA the unit of life. (foodshareonline.com)
- DNA is used by researchers as a molecular tool to explore physical laws and theories, such as the ergodic theorem and the theory of elasticity . (wikipedia.org)
- Fingerprint Dive into the research topics of 'Molecular cloning of complementary DNA encoding the avian receptor for vitamin D'. Together they form a unique fingerprint. (elsevier.com)
- Molecular cloning of complementary DNA encoding the avian receptor for vitamin D . Science , 235 (4793), 1214-1217. (elsevier.com)
- Sequencing and expression of complementary DNA for the general transcription factor BTF3. (nih.gov)
- The DNA β associated with Cotton leaf curl virus is responsible for symptom expression of a devastating disease in Pakistan. (apsnet.org)
- Complementary DNA cloning and constitutive expression of cytoc. (mysciencework.com)
- Complementary DNA cloning and constitutive expression of cytochrome P450 1C1 in the gills of carp (Cyprinus carpio). (mysciencework.com)
- It is hoped that this cloned DNA can be used as a probe to study the expression and structure of the ACTH/LPH gene. (uthscsa.edu)
- We examined the cell proliferation effect and mechanism of C. concinna -derived cryptocaryone (CPC) on oral cancer cells in terms of cell viability, apoptosis, reactive oxygen species (ROS), mitochondrial depolarization, and DNA damage. (biomedcentral.com)
- Moreover, γH2AX flow cytometry showed DNA damage in CPC-treated Ca9-22 cells. (biomedcentral.com)
- CPC-induced cell responses in terms of cell viability, apoptosis, oxidative stress, and DNA damage were rescued by N-acetylcysteine pretreatment, suggesting that oxidative stress plays an important role in CPC-induced death of oral cancer cells. (biomedcentral.com)
- Transfection of BEAS-2B cells with RIG-I complementary DNA resulted in the upregulation of STAT1. (ersjournals.com)
- Within eukaryotic cells, DNA is organized into long structures called chromosomes . (wikipedia.org)
- The technique was applied to cells infected with simian sarcoma-leukemia virus, where the virus-specific RNA was detected by hybridization to simian sarcoma-leukemia virus 3H-labeled complementary DNA. (semanticscholar.org)
- 2006). Systematic study of the effect of exocyclic bulky aromatic groups on the DNA stability allowed us to identify novel dG analogs that considerably improve oligonucleotide hybridization properties. (jbsdonline.com)
- That makes the PDZ-dG monomer a potentially useful tool for creation of various DNA hybridization methods, especially for detection of point mutations. (jbsdonline.com)
- If the complementary DNA is labeled with a compound that fluoresces, then the binding of the fluorescent probe can actually be visualized using a microscope . (jrank.org)
- Subcellular distribution of signal recognition particle and 7SL-RNA determined with polypeptide-specific antibodies and complementary DNA probe. (ucsf.edu)
- the free base of zebularine) and 6-methylfuranopyrimidinone (MefP), were prepared as dNTPs and evaluated as substrates for T7 and Phi29 DNA polymerases that lacked editor function. (jefferson.edu)
- In contrast, Bst 2.0, SIII and BIOTAQ™ DNA polymerases seem to display a loop-out mechanism possibly due to the flexible glycerol linker used instead of deoxyribose. (rsc.org)
- The majority of genetic information is stored within individual DNA molecules, although it is found in other cellular locations as well. (reference.com)
- DNA molecules are the first levels of storage for genetic material. (reference.com)
- Although viruses share several features with living organisms, such as the presence of genetic material (DNA or RNA), they are not considered to be alive. (encyclopedia.com)
- Criteria are presented by which to identify legitimate junk DNA, and to try to decipher the genetic clues of how genomes function now and in the past, when rates of change of genomes may have been very different. (creation.com)
- The structure of the DNA double helix . (wikipedia.org)
- The sugar-phosphate backbones of DNA are on the outside of the double helix. (news-medical.net)
- The three-dimensional structure of the duplex solved by NMR located both, the hydrophobic permethylated glucose and the nucleobase, inside the DNA helix as in the case of glucose-nucleobase pairs. (rsc.org)
- 14 Recently, we have placed a 6-deoxyglucose-guanine pair inside a DNA double helix to study this type of interaction. (rsc.org)
- Zaliznyak T., Bonala R., Johnson F., de los Santos C. (2006) Structure and Stability of Duplex DNA Containing the 3-(Deoxyguanosin-N 2 )--yl)-2-acetylaminofluorene (dG(N 2 )-AAF) Lesion: A Bulky Adduct that Persists in Cellular DNA. (jbsdonline.com)
- This distortion did not result in significant bending of the DNA, a result which was confirmed by gel electrophoresis studies of multimers of a 21-mer duplex containing the cross-link. (rcsb.org)
- The last decade of the 20th century has seen an explosion in research into the structure and function of the DNA in genomes of a wide range of organisms. (creation.com)
- Junk or 'selfish' DNA is believed to be largely parasitic in nature, persisting in the genomes of higher organisms as 'evolutionary remnants' by their ability to reproduce and spread themselves, or perhaps because they have supposedly mutated into a function the cell can use. (creation.com)