DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Ganoderma: A genus of fungi in the family Ganodermataceae, order POLYPORALES, containing a dimitic hyphal system. It causes a white rot, and is a wood decomposer. Ganoderma lucidum (REISHI) is used in traditional Chinese medicine (MEDICINE, CHINESE TRADITIONAL).Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Clostridium chauvoei: A species of gram-positive bacteria in the family Clostridiaceae isolated from infected CATTLE; SHEEP; and other animals. It causes blackleg in cattle and sheep and is transmitted through soil-borne spores.North CarolinaReagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.RNA Replicase: An enzyme that catalyses RNA-template-directed extension of the 3'- end of an RNA strand by one nucleotide at a time, and can initiate a chain de novo. (Enzyme Nomenclature, 1992, p293)Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.DNA-Directed RNA Polymerases: Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.RNA Polymerase II: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Systemic Inflammatory Response Syndrome: A systemic inflammatory response to a variety of clinical insults, characterized by two or more of the following conditions: (1) fever >38 degrees C or HYPOTHERMIA 90 beat/minute; (3) tachypnea >24 breaths/minute; (4) LEUKOCYTOSIS >12,000 cells/cubic mm or 10% immature forms. While usually related to infection, SIRS can also be associated with noninfectious insults such as TRAUMA; BURNS; or PANCREATITIS. If infection is involved, a patient with SIRS is said to have SEPSIS.Leukocytosis: A transient increase in the number of leukocytes in a body fluid.Sepsis: Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Multiple Organ Failure: A progressive condition usually characterized by combined failure of several organs such as the lungs, liver, kidney, along with some clotting mechanisms, usually postinjury or postoperative.Pancreatitis: INFLAMMATION of the PANCREAS. Pancreatitis is classified as acute unless there are computed tomographic or endoscopic retrograde cholangiopancreatographic findings of CHRONIC PANCREATITIS (International Symposium on Acute Pancreatitis, Atlanta, 1992). The two most common forms of acute pancreatitis are ALCOHOLIC PANCREATITIS and gallstone pancreatitis.Leukocyte Count: The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Gels: Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Silicon Dioxide: Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.Volatile Organic Compounds: Organic compounds that have a relatively high VAPOR PRESSURE at room temperature.Nanopores: Small holes of nanometer dimensions in a membrane, that can be used as single molecule detectors. The pores can be biological or synthetic.DNA Damage: Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.DNA-Formamidopyrimidine Glycosylase: A DNA repair enzyme that is an N-glycosyl hydrolase with specificity for DNA-containing ring-opened N(7)-methylguanine residues.Anticodon: The sequential set of three nucleotides in TRANSFER RNA that interacts with its complement in MESSENGER RNA, the CODON, during translation in the ribosome.GuanineMicrococcaceae: A family of bacteria ranging from free living and saprophytic to parasitic and pathogenic forms.Salmon: Fish of the genera ONCORHYNCHUS and Salmo in the family SALMONIDAE. They are anadromous game fish, frequenting the coastal waters of both the North Atlantic and Pacific. They are known for their gameness as a sport fish and for the quality of their flesh as a table fish. (Webster, 3d ed).Fish Diseases: Diseases of freshwater, marine, hatchery or aquarium fish. This term includes diseases of both teleosts (true fish) and elasmobranchs (sharks, rays and skates).Actinomycetales Infections: Infections with bacteria of the order ACTINOMYCETALES.Salmonidae: A family of anadromous fish comprising SALMON; TROUT; whitefish; and graylings. They are the most important food and game fishes. Their habitat is the northern Atlantic and Pacific, both marine and inland, and the Great Lakes. (Nelson: Fishes of the World, 1976, p97)Morale: The prevailing temper or spirit of an individual or group in relation to the tasks or functions which are expected.Gram-Positive Bacteria: Bacteria which retain the crystal violet stain when treated by Gram's method.Chromosomes, Artificial, Bacterial: DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Patient Access to Records: The freedom of patients to review their own medical, genetic, or other health-related records.Chromosomes, Artificial, Yeast: Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Online Systems: Systems where the input data enter the computer directly from the point of origin (usually a terminal or workstation) and/or in which output data are transmitted directly to that terminal point of origin. (Sippl, Computer Dictionary, 4th ed)Contig Mapping: Overlapping of cloned or sequenced DNA to construct a continuous region of a gene, chromosome or genome.Polynucleotide 5'-Hydroxyl-Kinase: An enzyme that catalyzes the transfer of a phosphate group to the 5'-terminal hydroxyl groups of DNA and RNA. EC 2.7.1.78.RNA, Catalytic: RNA that has catalytic activity. The catalytic RNA sequence folds to form a complex surface that can function as an enzyme in reactions with itself and other molecules. It may function even in the absence of protein. There are numerous examples of RNA species that are acted upon by catalytic RNA, however the scope of this enzyme class is not limited to a particular type of substrate.Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.RNA Ligase (ATP): An enzyme that catalyzes the conversion of linear RNA to a circular form by the transfer of the 5'-phosphate to the 3'-hydroxyl terminus. It also catalyzes the covalent joining of two polyribonucleotides in phosphodiester linkage. EC 6.5.1.3.PolynucleotidesBinding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.

Detection of Chlamydia pneumoniae but not cytomegalovirus in occluded saphenous vein coronary artery bypass grafts. (1/38768)

BACKGROUND: A causal relation between atherosclerosis and chronic infection with Chlamydia pneumoniae and/or cytomegalovirus (CMV) has been suggested. Whether the unresolved problem of venous coronary artery bypass graft occlusion is related to infection with C pneumoniae and/or CMV has not been addressed. METHODS AND RESUTLS: Thirty-eight occluded coronary artery vein grafts and 20 native saphenous veins were examined. Detection of C pneumoniae DNA was performed by use of nested polymerase chain reaction (PCR). Homogenisates from the specimen were cultured for identification of viable C pneumoniae. Both conventional PCR and quantitative PCR for detection of CMV DNA were applied. Differential pathological changes (degree of inflammation, smooth muscle cell proliferation [MIB-1]) were determined and correlated to the detection of both microorganisms. C pneumoniae DNA could be detected in 25% of occluded vein grafts. Viable C pneumoniae was recovered from 16% of occluded vein grafts. Except for 1 native saphenous vein, all control vessels were negative for both C pneumoniae detection and culture. All pathological and control specimens were negative for CMV DNA detection. Pathological changes did not correlate with C pneumoniae detection. CONCLUSIONS: Occluded aorto-coronary venous grafts harbor C pneumoniae but not CMV. The detection of C pneumoniae in occluded vein grafts warrants further investigation.  (+info)

Acinetobacter bacteremia in Hong Kong: prospective study and review. (2/38768)

The epidemiological characteristics of 18 patients with acinetobacter bacteremia were analyzed. Patients (mean age, 55.5 years) developed bacteremia after an average of 14.1 days of hospitalization. Fifteen of 16 patients survived bacteremia caused by Acinetobacter baumannii. Cultures of blood from the remaining two patients yielded Acinetobacter lwoffii. Most patients (78%) resided in the general ward, while four patients (22%) were under intensive care. Genotyping by arbitrarily primed polymerase chain reaction analysis and the temporal sequence of isolation were more useful than phenotyping by antimicrobial susceptibility in the determination of the source of bacteremia, and the intravascular catheter was the leading infection source (39% of cases). The possibility of an association of glucose with the pathogenesis of acinetobacter infection was raised.  (+info)

Legionnaires' disease on a cruise ship linked to the water supply system: clinical and public health implications. (3/38768)

The occurrence of legionnaires' disease has been described previously in passengers of cruise ships, but determination of the source has been rare. A 67-year-old, male cigarette smoker with heart disease contracted legionnaires' disease during a cruise in September 1995 and died 9 days after disembarking. Legionella pneumophila serogroup 1 was isolated from the patient's sputum and the ship's water supply. Samples from the air-conditioning system were negative. L. pneumophila serogroup 1 isolates from the water supply matched the patient's isolate, by both monoclonal antibody subtyping and genomic fingerprinting. None of 116 crew members had significant antibody titers to L. pneumophila serogroup 1. One clinically suspected case of legionnaires' disease and one confirmed case were subsequently diagnosed among passengers cruising on the same ship in November 1995 and October 1996, respectively. This is the first documented evidence of the involvement of a water supply system in the transmission of legionella infection on ships. These cases were identified because of the presence of a unique international system of surveillance and collaboration between public health authorities.  (+info)

Classification of thermophilic streptomycetes, including the description of Streptomyces thermoalcalitolerans sp. nov. (4/38768)

A polyphasic taxonomic study was undertaken to clarify relationships within and between representative thermophilic alkalitolerant streptomycetes isolated from soil and appropriate marker strains. The resultant data, notably those from DNA-DNA relatedness studies, support the taxonomic integrity of the validly described species Streptomyces thermodiastaticus, Streptomyces thermoviolaceus and Streptomyces thermovulgaris. However, the genotypic and phenotypic data clearly show that Streptomyces thermonitrificans Desai and Dhala 1967 and S. thermovulgaris (Henssen 1957) Goodfellow et al. 1987 represent a single species. On the basis of priority, S. thermonitrificans is a later subjective synonym of S. thermovulgaris. Similarly, 10 out of the 11 representative thermophilic alkalitolerant isolates had a combination of properties consistent with their classification as S. thermovulgaris. The remaining thermophilic alkalitolerant isolate, Streptomyces strain TA56, merited species status. The name Streptomyces thermoalcalitolerans sp. nov. is proposed for this strain. A neutrophilic thermophilic isolate, Streptomyces strain NAR85, was identified as S. thermodiastaticus.  (+info)

Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively. (5/38768)

Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively.  (+info)

Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov. (6/38768)

The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as 'biotype A' of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov. corresponds to [P.] haemolytica biogroups 3A-3H and the beta-glucosidase and meso-inositol-positive strains of [P.] haemolytica biogroup 9. All typable strains within M. glucosida belong to serotype 11. Mannheimia ruminalis sp. nov. consists of strains previously classified as Bisgaard taxon 18 and [P.] haemolytica biogroup 8D. Finally, Mannheimia varigena sp. nov. includes [P.] haemolytica biogroup 6 as well as Bisgaard taxon 15 and Bisgaard taxon 36. The type strains are NCTC 9380T (M. haemolytica), ATCC 49244T (M. granulomatis), CCUG 38457T = P925T (M. glucosida), CCUG 38470T = HPA92T (M. ruminalis) and CCUG 38462T = 177T (M. varigena).  (+info)

Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization. (7/38768)

The phylogenetic relationships of 49 Acinetobacter strains, 46 of which have previously been classified into 18 genomic species by DNA-DNA hybridization studies, were investigated using the nucleotide sequence of gyrB, the structural gene for the DNA gyrase B subunit. The phylogenetic tree showed linkages between genomic species 1 (Acinetobacter calcoaceticus), 2 (Acinetobacter baumannii), 3 and TU13; genomic species 6, BJ15, BJ16 and BJ17; genomic species 5, BJ13 (synonym of TU14) and BJ14; genomic species 7 (Acinetobacter johnsonii), 10 and 11; and genomic species 8 and 9. The phylogenetic grouping of Acinetobacter strains based on gyrB genes was almost congruent with that based on DNA-DNA hybridization studies. Consequently, gyrB sequence comparison can be used to resolve the taxonomic positions of bacterial strains at the level of genomic species. However, minor discrepancies existed in the grouping of strains of genomic species 8, 9 and BJ17. The phylogenetic tree for these strains was reconstructed from the sequence of rpoD, the structural gene for the RNA polymerase sigma 70 factor. The latter tree was 100% congruent with the grouping based on DNA-DNA hybridization. The reliability of DNA-DNA hybridization may be superior to that of sequence comparison of a single protein-encoding gene in resolving closely related organisms since the former method measures the homologies between the nucleotide sequences of total genomic DNAs. Three strains that have not been characterized previously by DNA-DNA hybridization seem to belong to two new genomic species, one including strain ATCC 33308 and the other including strains ATCC 31012 and MBIC 1332.  (+info)

Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. (8/38768)

Eight Gram-negative, aerobic, pointed and budding bacteria were isolated from various depths of the hypersaline, heliothermal and meromictic Ekho Lake (Vestfold Hills, East Antarctica). The cells contained storage granules and daughter cells could be motile. Bacteriochlorophyll a was sometimes produced, but production was repressed by constant dim light. The strains tolerated a wide range of temperature, pH, concentrations of artificial seawater and NaCl, but had an absolute requirement for sodium ions. Glutamate was metabolized with and without an additional source of combined nitrogen. The dominant fatty acid was C18:1; other characteristic fatty acids were C18:2, C12:0 2-OH, C12:1 3-OH, C16:1, C16:0 and C18:0. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G+C base composition was 62-64 mol%. 16S rRNA gene sequence comparisons showed that the isolates were phylogenetically close to the genera Antarctobacter, 'Marinosulfonomonas', Octadecabacter, Sagittula, Sulfitobacter and Roseobacter. Morphological, physiological and genotypic differences to these previously described and distinct genera support the description of a new genus and a new species, Roseovarius tolerans gen. nov., sp. nov. The type strain is EL-172T (= DSM 11457T).  (+info)

A different idea would be to gel purify your DNA. Assuming your plasmid is small (,7kb), you can gel purify your DNA on a low density agarose gel (~0.7%) . The genomic DNA which is large will be retained near the top while the plasmid DNA will migrate a little further into the gel. Excise the genomic DNA from the gel, extract the DNA by one of the old gel extraction protocol (do not use column, as the will only retain fragments below 10kb). ...
For a class of 30 students or for 6 separate teacher demonstrations. Excellent for all levels of teaching. Demonstrates the DNA extraction process of freeze-dried E. coli cells. Cell walls are broken with a detergent, and the DNA is extracted onto a spooling rod. The exercise helps students visualiz...
Deoxyribonucleic acid (DNA) is a molecule that includes the genetic instructions worn in the development and functioning of all known living organisms and many viruses.
CiteWeb id: 19830000003. CiteWeb score: 27848. DOI: 10.1016/0003-2697(83)90418-9. A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described. DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers. Over 70% of the precursor triphosphate is routinely incorporated into complementary DNA, and specific activities of over 109 dpm/μg of DNA can be obtained using relatively small amounts of precursor. These "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.. Links: ...
Deoxyribonucleic acid synthesis in Escherichia coli infected with some deoxyribonucleic acid polymerase-less mutants of bacteriophage T4.
The Magnetic Beads Genomic DNA Extraction Kit Blood was designed specifically for efficient genomic DNA purification from blood and buffy coat. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system.
On Tuesday, We did agarose gel electrophoresis for PCR samples to check for amplification 10:08, 23 October 2012. we did not get any amplification in DNA samples however, we got the positive control band. We made an interpretation from the gel that may be primers did not bind at their specific sites or may be there is some problem in DNA sample. Since we are running out of our DNA sample, we decided to do the DNA extraction.This time we will be comparing two different protocols on parallel. One that we used before i.e, DNA extraction for E.coli chromosome and other that group 25 used which is, Bacterial Genomic DNA extraction protocol. On Wednesday, we did the culture to grow the E. coli. cells in four flasks and incubated in shaker for overnight. On Thursday, since we did not have enough killing buffer we did the extraction with the genomic DNA extraction protocol only. We put rest of the three culture flasks at 4ºC.We will do agarose gel electrophoresis for these DNA samples in our next lab. ...
AccuPrep® GMO DNA Extraction Kit allows the extraction of DNA from agricultural products such as beans, corn, and rice or from processed foods such as bean sprouts, tofu and condensed milk, for the detection of GMOs. The kit includes all reagents required for extraction and in addition uses a column type extraction system to allow a more rapid, more convenient extraction of higher-quality DNA when compared to conventional methods. A high amount of high-purity DNA can be extracted; for example, 20 ~ 40 ug of DNA for 1 g of powdered bean, with an A260/A280 ratio of over ...
EasyPure® Plasmid MiniPrep Kit,Plasmid DNA Purification and E. Coli Medium,Nucleic Acid Purification,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionEasyPure® P
The DSMZ is one of the largest biological ressource centers worldwide.Its collections currently comprise more than 50,000 items, including about 27,000 different bacterial and 4,000 fungal strains, 800 human and animal cell lines, 700 plant cell lines, 1,400 plant viruses and antisera, and 13,000 different types of bacterial genomic DNA.. All biological materials accepted in the DSMZ collection are subject to extensive quality control and physiological and molecular characterization by our central services. In addition, DSMZ provides an extensive documentation and detailed diagnostic information on the biological materials. The unprecedented diversity and quality management of its bioressources render the DSMZ an internationally reknown supplier for science, diagnostic laboratories, national reference centers, as well as industrial partners ...
Miniprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 1~5 ml of LB broth and the expected DNA yield is 20~30 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and midiprep - Plasmid DNA Extraction (Miniprep) - AbVideo™ - Support - Abnova
НИИ атеросклероза: научные исследования, публикации сотрудников института (abstracts, full-text.), дискуссионный клуб, посвященный вопросам механизмов атерогенеза.
Does anyone know if any really good Plasmid DNA purifications kits available on the market? Looking for max DNA recovery. Thanks, Claude Baker ...
QIAGEN has established a partnership with Aldevron, a company that specializes in custom plasmid DNA preparation services. Through this partnership, Aldevron is offering customers the possibility to order standard plasmid DNA preparation services performed using high-quality QIAGEN plasmid purification products. This service offers three different scales of endotoxin-free* plasmid DNA. This standardized service is only available via the Web site |span style=text-decoration: underline;>www.plasmid.com|/span>. |br /> |br /> Learn more:
We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, well assume that you are happy to receive all cookies on the Greiner Bio-One website. However, if you would like to, you can change your cookie settings at any time ...
DNA - Deoxyribonucleic Acid and RNA - Ribonucleic Acid: Biology Assignment Help, Homework Help, Project Help and Instant solution for DNA-RNA with qualified biology experts.
I m doing CHIP assays and during DNA purification and after treatment with 100 % and 70% ethanol and centrifugation I see pellet in all my samples. Is this normal to see the pellet-DNA? I am thinking that maybe I should reduce the amount of chromatin that I incubate with the antibody ...
BIOS researchers realized that the ubiquitous, ecologically important marine bacterium "SAR11" was getting short-changed in bacterial census data. With collaborators, they improved a common DNA-based method for measuring bacterial diversity in marine environments.. ...
on Deoxyribonucleic Acid.. ___________________________________________________________. For life to continue, new cells need to be produced. For this to happen, DNA needs to make a copy of itself to make new cells. The new cells need to have the correct information. Then the cell can divide and the new cell will have an exact copy of the original DNA. When DNA makes a copy of itself it is called DNA Replication. The nitrogen bases of the DNA ladder are bonded by hydrogen bonds. Those bonds break and . . . .. Click on the screen to watch my powerpoint on ...
View Notes - Molecular I pre-lab from BIOSC 0060 at Pittsburgh. Molecular I Pre-lab 1. What is a plasmid? How is it different from genomic or chromosomal DNA? a. A plasmid is an extra chromosomal DNA
In biological systems, nucleic acids (https://en.wikipedia.org/wiki/Nucleic_acid) contain information which is used by a living cell to construct specific proteins. The sequence of nucleobases on a nucleic acid strand is translated by cell machinery into a sequence of amino acids making up a protein strand.. The different nucleobases that make up DNA are - Adenine. - Cytosine. - Guanine. - ...
In biological systems, nucleic acids (https://en.wikipedia.org/wiki/Nucleic_acid) contain information which is used by a living cell to construct specific proteins. The sequence of nucleobases on a nucleic acid strand is translated by cell machinery into a sequence of amino acids making up a protein strand.. The different nucleobases that make up DNA are ...
DNA contains the instructions for life, encoded within genes. Within all cells, DNA is organised into very long lengths known as chromosomes.
This miniprep Plasmid Kit was designed for plasmid DNA purification from 1-7 ml of cultured bacterial cells. For processing larger volumes, the Presto™ Midi Plasmid Kit is also available.
DNA is the hereditary material found in humans and other organisms. DNA, found in nucleus of a cell is known as nuclear DNA and found in mitochondria is known as mitochondrial DNA (NIH, 2014). Genes and chromosomes are made up of DNA and human body contains 20,000 to 25,000 genes (NIH, 2014). Building block of…
This section describes considerations for isolation and quantification of both genomic DNA from different sample sources and plasmid DNA. It also deals with common plasmid DNA procedures, including how to make and transform competent cells, how to culture and handle plasmid-containing cells, and commonly used techniques for analysis of genomic DNA.
Cell and molecular biology products for DNA purification, nucleic acid electrophoresis, PCR, plasmid DNA isolation, yeast research, and more.
The PureYield™ Plasmid Maxiprep System isolates transfection-quality plasmid DNA. Yields up to 1mg of plasmid DNA from 250ml of bacterial culture.
Read user reviews, compare products and contact manufacturers of PCR / Thermal Cycling products, including enzymes, RT PCR and DNA purification on SelectScience.
Read user reviews, compare products and contact manufacturers of PCR / Thermal Cycling products, including enzymes, RT PCR and DNA purification on SelectScience.
In addition, we nanodroped the purified plasmid samples to determine the DNA concentration. The following BioBrick parts were sequenced in both directions using BioBrick primers VF and VR2 (note that two single colonies from each transformations were used): ...
Page contains details about PEG-b-P4VP micelles-circular plasmid DNA cyclic beads-on-a-string structures . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
De bestuurder van NCDO is sinds maart 2017 drs. Jan Bouke Wijbrandi. Hij was van 2001 tot 2008 lid van de directie van Oxfam Novib.
Plasmid pDBTrp-LexABD-CRY2FL from Dr. Chandra Tuckers lab contains the insert LexABD-CRY2 and is published in ACS Synth Biol. 2014 Nov 21;3(11):832-8. doi: 10.1021/sb500291r. Epub 2014 Nov 5. This plasmid is available through Addgene.
Plasmid pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A from Dr. Loren Loogers lab contains the insert SF-Venus-iGluSnFR.S72A and is published in Nat Methods. 2018 Nov;15(11):936-939. doi: 10.1038/s41592-018-0171-3. Epub 2018 Oct 30. This plasmid is available through Addgene.
P4PS, E28A, V35T, E36D, V60I, I135V, T139TA, S162A, K173A, Q174K, D177E, T200A, Q207E, R211RK, F214L, H221Y, L228LH, V245Q, A272G, L283I, T286A, V292I, I293V, S322T, ...
V35I, I135L, E138A, S162A, I167IV, V179I, R211RKMT, H221Y, D237N, V245Q, A272P, A288S, P294T, I329L, Q334E, Y339F, R356K, I375V, T377L, T386I, K388R, ...
Several high frequency restriction fragment length polymorphisms (RFLPs) associated with the human gene for apolipoprotein B have been previously reported by Priestly et al. The EcoRI RFLP here was shown to be very strongly associated with the Ag(t/z) immunochemical polymorphism of human low density lipoproteins, allowing correct Ag(t/z) phenotyping of 17 (out of 17 tested) unrelated individuals. The Xbal RFLP was associated with the Ag(g/c) immunochemical polymorphism, permitting correct phenotyping of 14 (out of 17 tested) unrelated individuals. Its close association with an RFLP permitted localization of the Ag(t/z) polymorphism to the C-terminal end of the apolipoprotein B peptide, and allowed detailed discussion of its probable molecular basis. ...
My lab works on AbResistance and isolating R Plasmids without genomic DNA contamination, was a great issue.We wanted plasmid DNA without genomic DNA contamination as we wanted to check resistance gene on plasmid. We tried the plasmid isolation kit (Cat. No. - MG18Pl-25) and are extremely happy to get good plasmid yield. Moreover there is no genomic DNA contamination in it. We also used bacterial genomic DNA isolation kit (Cat. No. - MG18Ba-25). DNA is really pure and can be used for NGS and routine PCR work. Both plasmid isolation kit and bacterial genomic DNA isolation kit are fabulous, fast and efficient. Highly satisfied ...
Isolation of genomic DNA is an essential technique in modern research science, particularly molecular biology and biotechnology. Genomic DNA is purified from a multitude of sources including mammalian tissue, such as cheek cells (BE-303), plant cells or bacterial cells.. These kits use detergent lysis and precipitation to purify genomic DNA from onion or bacteria. Other plants or fruits can be used, such as strawberries. These kits do not utilize toxic agents, such as phenol or chloroform for genomic DNA extraction.. Agarose electrophoresis can be used to visualize the genomic DNA on an agarose gel.. Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teachers Guide and separate Students Guides.. ...
MagListoTM 5M Plant Genomic DNA Extraction Kit 는 magnetic nano bead와 MagListoTM를 이용하여 Plant sample (leaf, root, seed) 에서 Genomic DNA를 빠르게 추출할 수 있는 획기적인 제품입니다. Magnetic nano bead와 자석을 이용해 세포 분쇄물 중 Genomic DNA만을 분리시키고 농축 및 정제하는 과정을 거치기 때문에 원심분리기를 사용하는 방법에 비해 빠르게 DNA를 분리 할 수 있습니다. 본 제품은 mini, midi, maxi scale의 prep을 위해 별도의 kit를 구매하지 않고 한 가지의 kit를 이용해 모두 prep 할 수 있으며 midi나 maxi prep을 위해 별도의 vacuum system이나 air pressure system을 구비 할 필요가 없는 것이 장점입니다.
AccuPrep® Genomic DNA Extraction Kit for Biovac 96 Vacuum Manifold has been designed to quickly and conveniently extract genomic DNA from whole blood, buffy coat, lymphocytes, plasma, serum, body fluids, and cultured cells, simultaneously. The 96 samples can be handled without additional machinery such as a centrifuge. The genomic DNA is simply extracted with a vacuum pump and Biovac 96 Vacuum Manifold. This product is also available to other companies vacuum manifold system (QIAGEN, Promega and Axygen).
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
The GenElute Bacterial Genomic Kit provides a simple and convenient technique to isolate high quality DNA from both Gram(-) and Gram(+) bacteria. This kit combines the advantages of a silica-based system with a microspin format, eliminating the need for expensive resins and hazardous organic compounds.
PlantZol,Genomic DNA Purification,Nucleic Acid Purification,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionPlantZol provides an easy and fast method to isolate hi
Synonyms for restriction fragment in Free Thesaurus. Antonyms for restriction fragment. 1 word related to restriction fragment: fragment. What are synonyms for restriction fragment?
DNA replication. Computer artwork of a DNA (deoxyribonucleic acid) molecule replicating. DNA is composed of two strands twisted into a double helix. Before replication the strands separate from each other. Each strand then acts as a template for the formation of a new DNA molecule. This is known as semiconservative replication. DNA contains sections called genes, which encode the bodys genetic information. - Stock Image G110/0860
Hi! Guys: Help need for plasmid DNA purification. I am purifying plasmid DNA using Promega miniprepa DNA Purification System from Novablue cell transformated by Clontech pEGFP-N1 containing the insert. Some strange things happened, I really got the plasmid with insert, but allways companied by a 580 bp DNA band. Since the plasmid DNA is to be used for transfection, so I got to figure out what it is. Does anybody have experience about this? Please help ...
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NucleoBond kits for plasmid miniprep, midiprep, maxiprep, and endotoxin-free purification are gravity columns based on a patented anion exchange technology. The plasmid DNA obtained is highly pure and suitable for a variety of downstream applications
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There can be a few different reasons why you observe additional bands in your digest. For a discussion on this topic please refer to the video above.
The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.
The plasmid MLST isolate database contains data for plasmids and isolates representing incompatibility groups I1, HI1, HI2, F and N. The allele sequences and profiles are defined in the profiles/sequence definition database.. ...
thanks a lot I understood little bit , but in my case as told to me that the ordered nucleotide sequence will be incoroporated with the plasmid and then i have to to subclone it into E.coli TOF competent cell and then i have to plate it select for the colonies and then do the mini prep to get the plasmid and then I have to do the pcr... I really didnt understand How can I do the pcr with the plasmid (Although my gene of intreset in there). but my question is if i do by this process and I do the pcr Should I get the band on 1,4kb AND THEN I EXCISE IT AND CARRYOUT MY FURTHER PROCESS: IF i am right kindly tell me or wrong please guide me with your answer. I would be highly obliged to you, I am unableto understnd this concept ...
GenScripts industrial-grade Plasmid DNA can help your experiments achieve highly efficient cell transfection, helping to improve experimental outcomes in research areas such as protein expression, antibody production, and other research projects.
Smiths Detection, part of Smiths Group, plc, is developing a portable pathogen identification system based on technology called LATE PCR (Linear After The
DNA - A double helix DNA stands for Deoxyribonucleic acid.It is a nucleic which is used for storing information for long term in all living beings and some viruses. Base composition in DNA varies from one species to other but in all the cases the amount of adenine is equal to thymine and the amount
A plasmid is a small DNA molecule that is physically separate from, and can replicate independently of, chromosomal DNA within a cell. Plasmids are commonly used to multiply (make many copies of) …
Sample preparation includes the proprietary Pickpen® IMS which isolates the target organism and removes it from the food matrix and media which could interfere with PCR results ...
Sample preparation includes the proprietary Pickpen® IMS which isolates the target organism and removes it from the food matrix and media, which could interfere with PCR results ...
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DNA, also known as deoxyribonucleic acid, is a fundamental molecule found in all living things. It serves as the basis for heredity, specifying which traits are passed on from parents to children through the generations. It also contains instructions for our body cells to perform their specific functions. Structure In humans, most of the DNA…. Details ...
From 1st of January 2018 onwards, the BCCM/LMBP Plasmid Collection will officially be known as BCCM/GeneCorner. Find out in the video what this means for you!
Page contains details about plasmid DNA-loaded lipo-nanoparticles . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
100% واي أيزوليت ..بروتين بنائي متفوق. صممت تركيبة المصل النقي المركّزة لغرض تحقيق هدفين مهمين وهما: * توفير تركيز حقيقي عالي من البروتين في تركيبة ذات نكهة، *والسماح للمستخدم بتناول بروتين يساعد في إحداث أهم وأسرع تدفق للأحماض الأمينية إلى العضلات وبالتالي يعطي أقوى دفعة لتركيبة بنائية. ويتميز مصدر البروتين الخاص في تركيبة (واي أيزوليت 100%) بوجود إمكانية حركية فريدة في امتصاص الأحماض الأمينية ذلك أن بيبيدات المصل تنقل بفعالية إلى مجرى الدم و العضلات. فالامتصاص الأسرع والأفضل للأحماض الأمينية يعطي بناء عضلي أفضل. يحتوي هذا المنتج على نسبة منخفضة من ...
TY - JOUR. T1 - Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents. AU - Doebbeling, Bradley. AU - Pfaller, M. A.. AU - Hollis, R. J.. AU - Boyken, L. D.. AU - Pignatari, A. C.. AU - Herwaldt, L. A.. AU - Wenzel, R. P.. PY - 1992/1. Y1 - 1992/1. N2 - Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of ,10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local ...
Fifty-nine Staphylococcus aureus isolates and 1 isolate of Staphylococcus intermedius were typed by investigators at eight institutions by using either antibiograms, bacteriophage typing, biotyping, immunoblotting, insertion sequence typing with IS257/431, multilocus enzyme electrophoresis, restriction analysis of plasmid DNA, pulsed-field or field inversion gel electrophoresis, restriction analysis of PCR-amplified coagulase gene sequences, restriction fragment length polymorphism typing by using four staphylococcal genes as probes, or ribotyping. Isolates from four well-characterized outbreaks (n = 29) and a collection of organisms from two nursing homes were mixed with epidemiologically unrelated stock strains from the Centers for Disease Control and Prevention. Several isolates were included multiple times either within or between the sets of isolates to analyze the reproducibilities of the typing systems. Overall, the DNA-based techniques and immunoblotting were most effective in grouping ...
Bacterial insertion sequences are the simplest form of autonomous mobile DNA. It is unknown whether they need to have beneficial effects to infect and persist in bacterial populations, or whether horizontal gene transfer suffices for their persistence. We address this question by using branching process models to investigate the critical, early phase of an insertion sequence infection. We find that the probability of a successful infection is low and depends linearly on the difference between the rate of horizontal gene transfer and the fitness cost of the insertion sequences. Our models show that the median time to extinction of an insertion sequence that dies out is very short, while the median time for a successful infection to reach a modest population size is very long. We conclude that horizontal gene transfer is strong enough to allow the persistence of insertion sequences, although infection is an erratic and slow process. ...
Roychoudhury, R and Bloch, D P., "Studies on deoxyribonucleic acid polymerase from ehrlich ascites tumor cells. II. Factors influencing primer and template requirement of deoxyribonucleic acid polymerase." (1969). Subject Strain Bibliography 1969. 746 ...
Widespread use of DNA restriction fragment length polymorphism (RFLP) to differentiate strains of Mycobacterium tuberculosis to monitor the transmission of tuberculosis has been hampered by the need to culture this slow-growing organism and by the level of technical sophistication needed for RFLP typing. We have developed a simple method which allows simultaneous detection and typing of M. tuberculosis in clinical specimens and reduces the time between suspicion of the disease and typing from 1 or several months to 1 or 3 days. The method is based on polymorphism of the chromosomal DR locus, which contains a variable number of short direct repeats interspersed with nonrepetitive spacers. The method is referred to as spacer oligotyping or "spoligotyping" because it is based on strain-dependent hybridization patterns of in vitro-amplified DNA with multiple spacer oligonucleotides. Most of the clinical isolates tested showed unique hybridization patterns, whereas outbreak strains shared the same ...
TY - JOUR. T1 - Evaluation of DNA extraction methods for the analysis of microbial community in biological activated carbon. AU - Zheng, Lu. AU - Gao, Naiyun. AU - Deng, Yang. PY - 2012/2/1. Y1 - 2012/2/1. N2 - It is difficult to isolate DNA from biological activated carbon (BAC) samples used in water treatment plants, owing to the scarcity of microorganisms in BAC samples. The aim of this study was to identify DNA extraction methods suitable for a long-term, comprehensive ecological analysis of BAC microbial communities. To identify a procedure that can produce high molecular weight DNA, maximizes detectable diversity and is relatively free from contaminants, the microwave extraction method, the cetyltrimethylammonium bromide (CTAB) extraction method, a commercial DNA extraction kit, and the ultrasonic extraction method were used for the extraction of DNA from BAC samples. Spectrophotometry, agarose gel electrophoresis and polymerase chain reaction (PCR)-restriction fragment length ...
Synonyms for coccobacilli in Free Thesaurus. Antonyms for coccobacilli. 4 words related to coccobacillus: Brucella, eubacteria, eubacterium, true bacteria. What are synonyms for coccobacilli?
View Notes - lab Write-up from ENGLISH 1011 at Berkeley. Jacob Zipperstein H. Bio Med January 28, 2009 Title: DNA Restriction Analysis (Lambda DNA) Purpose: The purpose of this lab is to analyze
Looking for online definition of Deoxyribonucleic in the Medical Dictionary? Deoxyribonucleic explanation free. What is Deoxyribonucleic? Meaning of Deoxyribonucleic medical term. What does Deoxyribonucleic mean?
Pontiroli, Alessandra, Travis, Emma Rachel, Sweeney, F. P., Porter, David, Gaze, William H., Mason, Sam, Hibberd, V., Holden, Jennifer, Courtenay, Orin and Wellington, E. M. H.. (2011) Pathogen quantitation in complex matrices : a multi-operator comparison of DNA extraction methods with a novel assessment of PCR inhibition. PLoS One, Vol.6 (No.3). Article number e17916. ISSN 1932-6203 ...
This volume is developed on the broad theme of plant-associated bacteria. It is envisioned as a resource volume for researchers working with beneficial and harmful groups of bacteria associated with c
Press release - Autoantibodies may be created in response to bacterial DNA April 27, 2009 - Autoimmune diseases have long been regarded as illnesses in which the immune system creates autoantibodies to attack the body itself. But today, researchers at the California non-profit Autoimmunity Research Foundationautoimmune diseaseHuman Microbiomeautoimmune diseaseAutoimmunityautoimmune diseaseautoantibodiesautoimmune diseaseautoimmuneAutoimmunityautoimmune diseaseAutoimmunityAutoimmune disease…
In this section: Introduction , The Omni-Clean™ System , The Omni-Pure™ Plasmid Purification System , The Omni-Pure™ Genomic DNA Purification System , Viral DNA & RNA Purification , Microbial DNA Purification , Plant DNA Purification ...
In this section: Introduction , The Omni-Clean™ System , The Omni-Pure™ Plasmid Purification System , The Omni-Pure™ Genomic DNA Purification System , Viral DNA & RNA Purification , Microbial DNA Purification , Plant DNA Purification ...
Journal of Chemometrics ; Volume 22. p. 309-322. 2008. Zimonja, Monika; Rudi, Knut; Trosvik, Pål; Næs, Tormod. A comprehensive understanding of factors that influence microbial competition and cooperation, their diversity and processes will be greatly beneficial in many research areas. Current tools for microflora determinations are far from suitable for high-throughput monitoring of development in complex microbial communities. Here, we describe the application of a calibration free method, multivariate curve resolution with alternating least squares (MCR-ALS), for identification and quantification of different microbes in mixture samples. The idea is to utilize MCR-ALS to enable close monitoring of ecology in a variety of microbial communities. The data from two designed experiments consisting of DNA sequence spectra measured on mixtures were analysed with MCR-ALS using no prior information on the data except for appropriate constraints, such as non-negativity and closure. The results were ...
The soil organism Bacillus subtilis has the ability to take up DNA from its environment and, provided there is homology, recombine it into its genome. This process is called transformation. In order for the bacterium to be transformed it must be in a specific physiological state called competence. During the competent state specific proteins are synthesized which are required for the binding and transport of the DNA molecule into the competent cell. It has been found that as transformability increases many competence specific proteins localize to the poles of the rod shaped Bacillus subtilis bacterium and as transformability declines the competence proteins delocalize. These proteins colocalize and interact and seem to form a complex that helps internalize the DNA molecule, preferentially at the poles of the cell. Jeanette Hahn s focus is understanding how the polar localization and delocalization of the competence proteins occurs. Localization seems to occur via a diffusion and capture ...
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This The EZgeneTM 96-well plasmid kit provides an easy and fast method for isolating high quality plasmid DNA in a high through put format The key to this system is Biomiga s ezbind matrix that avidly but reversibly binds DNA under optimized buffer condition while proteins and other unwanted
Transformation is a process by which recipient calls acquire genes from free DNA molecules in the surrounding medium". In other words, this is the transfer of genes from free DNA molecules in the surrounding medium into a recipient cell. Transformation starts the uptake of a DNA fragment from the neighbouring medium by the recipients cell and ends with one strand of donor DNA substituting the homologous segment in the recipient DNA. It has to be noted that even amongst cells of a species that is generally capable of transformation, only some cells in a growing population are efficient in the uptake of the free genetic information. The main experiment linked to this was Griffiths work with smooth virulent (Deadly) strain of Streptococcus pneumonia and rough (non-deadly) strain on Mice. The smooth appearance of the deadly colony is caused by a carbohydrate coating on the surface of each cell, which acts as protection against the mouses immune system and therefore allows the colony to thrive. ...
Hi Doctors Had some tests done a few weeks ago. Here are some of my results:- SPECIAL PATHOLOGY Chlamydia Trachomatis DNA(PCR) Not detected N. Gonorrhoeae DNA (PCR) Not detected Mycoplasm...
Currently, PCR (Polymerase Chain Reaction) sequencer is the most favored and well know method. The PCR can amplify bacterial DNA a thousand times if necessary, any routine diagnosis requires only a min of 10 - 80 bacteria, which means PCR has a very high sensitivity. This factor is exploited for countless diagnostic processes. PCR also doesnt require purification of the bacterial sample compared to older methods as it has specific sequences to identify bacterial DNA. Versatility of PCR has proven to be a big advantage, diagnosis of many dangerous diseases like tuberculosis, pneumonia etc are done with the intention to specify whether the strain is drug resistant. This greatly increases the success rate of treatment. The shortcomings of PCR are also eminent; PCR is susceptible to false positive report in presence of any contamination, it is also not useful in calculating the success of a treatment, as PCR recognizes dead and living cells as the same. Technical expertise is a major requirement in ...
The NucleoSpin Multi-96 Plus Plasmid Kits are designed for purifying up to 20 µg of high-copy plasmids from up to 5 ml E. coli overnight culture. DNA is ready to use for PCR, Southern blotting, or any kind of enzymatic reaction.
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The prevalence of antibiotic resistance has resulted in the need for new approaches to be developed to combat previously easily treatable infections. Here we investigated the potential of the synthetic metallomolecules [Fe(2)L(3)](4+) and [Cu(2)(L)(2)](2+) as antibacterial agents.... [Fe(2)L(3)](4+) binds in the major groove and causes DNA coiling... The work described here shows that ... [Fe(2)L(3)](4+) is bactericidal for Bacillus subtilis and Escherichia coli. We demonstrate that [Fe(2)L(3)](4+) binds bacterial DNA in vivo and, strikingly, that it kills B. subtilis cells very rapidly. _IntJnlAntimicrobialAgents ...
This could be a few things: 1. Bad antibiotic - check to see if bacteria that dont harbor the same resistance will grow/not grow in the media or on the plate. 2. Something wrong with the method of DNA prep - try to prep other plasmids using the same kit/reagents. Try to prep plasmids with the same antibiotic resistance. 3. No plasmid to prep - Use a method of colony cracking where you take some of the culture, pop the bacteria and run it straight on a gel without prepping for clean plasmid. This will let you know if anything is there to prep. In general, clones should not be able to grow on a particular antibiotic if there is no plasmid expressing resistance ...
Obtaining full-length 16S rRNA gene sequences is important for generating accurate taxonomy assignments of bacteria, which normally is realized via clone library construction. However, the application of clone library has been hindered due to its limitations in sample throughput and in capturing minor populations (<1 % of total microorganisms). To overcome these limitations, a new strategy, two-step denaturing gradient gel electrophoresis (2S-DGGE), is developed to obtain full-length 16S rRNA gene sequences. 2S-DGGE can compare microbial communities based on its first-round DGGE profiles and generate partial 16S rRNA gene sequences (8-534 bp, Escherichia coli numbering). Then, strain-specific primers can be designed based on sequence information of bacteria of interest to PCR amplify their remaining 16S rRNA gene sequences (515-1541 bps, E. coli numbering). The second-round DGGE can confirm DNA sequence purity of these PCR products. Finally, the full-length 16S rRNA gene sequences can be ...
Silvestri, L. G. (Università Statale, Milan, Italy), and L. R. Hill. Agreement between deoxyribonucleic acid base composition and taxometric classification of gram-positive cocci. J. Bacteriol. 90:136-140. 1965.-It had been previously proposed, from taxometric analyses, that gram-positive, catalase-positive cocci be divided into two subgroups. Thirteen strains, representative of both subgroups, were examined for deoxyribonucleic acid (DNA) base composition, determined from melting temperatures. Per cent GC (guanine + cytosine/total bases) values fell into two groups: 30.8 to 36.5% GC and 69 to 75% GC. Strains with low per cent GC values belonged to the Staphylococcus aureus-S. saprophyticus-S. lactis taxometric subgroups, and those with high per cent GC values belonged to the S. roseus-S. afermentans subgroup. The hypothetical nature of any classification is emphasized, and, in the present work, the hypothesis derived from taxometric analyses of division into two subgroups is confirmed by the ...
A novel Ferrimonas species is described on the basis of phenotypic, chemotaxonomic and phylogenetic studies. Four halophilic organisms were isolated from marine sand and marine macroalgae samples by using high-pH marine agar 2216. An analysis of the nearly complete 16S rRNA gene sequences of these new isolates indicated that they were phylogenetically close (16S rRNA gene sequence similarity |99·5 %, gyrB gene sequence similarity |97·8 %), and were most closely related to Ferrimonas balearica (16S rRNA gene sequence similarity 97·1-97·3 %, gyrB gene sequence similarity 84·4-85·0 %). Chemotaxonomic data (major menaquinone MK7; major fatty acids C16 : 0 and C18 : 1 ω9c) supported the affiliation of the new isolates to the genus Ferrimonas. The results of physiological and biochemical tests allowed phenotypic differentiation of the isolates from F. balearica. It is therefore proposed that the new isolates represent a novel species with the name Ferrimonas marina sp. nov. and type strain A4D-4T (
A novel actinomycete strain, YIM 61503T, isolated from the stem of Maytenus austroyunnanensis, was characterized by using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain YIM 61503T belonged to the genus Jiangella and exhibited 16S rRNA gene sequence similarities of 98.8 and 98.6 % to Jiangella alkaliphila D8-87T and Jiangella gansuensis YIM 002T. The chemotaxonomic properties of strain YIM 61503T were consistent with those of the genus Jiangella: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid and MK-9(H4) was the predominant menaquinone. The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, iso-C14 : 0, C17 : 1 ω8c and anteiso-C17 : 0. The DNA G+C content was 71.9 mol%. Strain YIM 61503T was phenotypically distinct from recognized Jiangella species and was shown to belong to a separate genomic species based on DNA-DNA hybridization results. Thus, strain YIM 61503T is considered to represent a novel species of the genus Jiangella,
Bartonella vinsonii is a gram-negative bacteria from the genus of Bartonella which was isolated from dogs Rochalimaea vinsonii was reclassified to Bartonella vinsonii Bartonella vinsonii contains the two subspecies Bartonella vinsonii subsp. vinsonii and Bartonella vinsonii subsp. berkhofli. Bartonella vinsonii subsp. vinsonii has been isolated from voles and Bartonella vinsonii subsp. berkhofli was isolated from a dog with endocarditis. Bartonella vinsonii subsp. berkhoffii can cause diseases in humans. Those two subspecies are named after J. William Vinson and Herman A. Berkhoff. LPSN bacterio.net Straininfo of Bartonella vinsonii uniProt Bartonella vinsonii subsp. berkhofii subsp. nov., Isolated fromDogs; Bartonella vinsonii subsp. vinsonii; and EmendedDescription of Bartonella-vinsonii Cadenas, M. B.; Bradley, J.; Maggi, R. G.; Takara, M.; Hegarty, B. C.; Breitschwerdt, E. B. (2008). "Molecular Characterization of Bartonella vinsonii subsp. Berkhoffii Genotype III". Journal of Clinical ...
TY - JOUR. T1 - Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis. AU - Reed, C.. AU - Sturbaum, G. D.. AU - Hoover, P. J.. AU - Sterling, Charles R. PY - 2002. Y1 - 2002. N2 - Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.. AB - Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information ...
A bacterial DNA transposon. A transposable element (TE or transposon) is a DNA sequence that can change its position within a ... The DNA-transposase complex then inserts its DNA cargo at specific DNA motifs elsewhere in the genome, creating short TSDs upon ... A DNA polymerase fills in the resulting gaps from the sticky ends and DNA ligase closes the sugar-phosphate backbone. This ... In bacteria, transposons can jump from chromosomal DNA to plasmid DNA and back, allowing for the transfer and permanent ...
"Bacterial genetics". Nature. Macmillan Publishers Limited. Retrieved 8 November 2015.. *^ Chen I, Dubnau D (2004). "DNA uptake ... Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge ... Natural transformation is a bacterial adaptation for DNA transfer between two cells through the intervening medium. The uptake ... Bacterial conjugation has been extensively studied in Escherichia coli, but also occurs in other bacteria such as Mycobacterium ...
... natural bacterial transformation involves the transfer of DNA from one bacterium to another and integration of the donor DNA ... Chen I; Dubnau D (March 2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-249. doi:10.1038/ ... The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments ... All cells possess DNA, the hereditary material of genes, and RNA, containing the information necessary to build various ...
Setlow P (April 2007). "I will survive: DNA protection in bacterial spores". Trends in Microbiology. 15 (4): 172-80. doi: ... correctly described Clostridium botulinum as the bacterial source of the toxin. Thirty-four attendees at a funeral were ...
... , a bacterial histone-like DNA-binding protein. *Wu Hu (disambiguation), an ancient Chinese term for multiple groups in China ...
DNA Seq. 10 (6): 365-77. doi:10.3109/10425170009015604. PMID 10826693. Stolz JF, Oremland RS (1999). "Bacterial respiration of ...
Then, the DNA sequence can be inserted back to a random location of the genome. DNA transposons are a DNA segment that can move ... Check date values in: ,date= (help) Hausner, Georg; Hafez, Mohamed; Edgell, David R. (2014-03-10). "Bacterial group I introns: ... Plasmids of bacteria are a transferable genetic element through bacterial conjugation. This is a mechanism of horizontal gene ... Transposons (also called transposable elements) are DNA sequences that can move locations within a genome, which includes ...
Bacterial artificial chromosomes (BACs) are circular DNA molecules, usually about 7kb in length, that are capable of holding ... The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a ... Use the enzyme DNA ligase to seal the DNA fragments into the vector. This creates a large pool of recombinant molecules. These ... Extract and purify DNA. Digest the DNA with a restriction enzyme. This creates fragments that are similar in size, each ...
Other NAD-dependent enzymes include bacterial DNA ligases, which join two DNA ends by using NAD+ as a substrate to donate an ... Wilkinson A, Day J, Bowater R (2001). "Bacterial DNA ligases". Mol. Microbiol. 40 (6): 1241-8. doi:10.1046/j.1365-2958.2001. ... This contrasts with eukaryotic DNA ligases, which use ATP to form the DNA-AMP intermediate. In recent years, NAD+ has also been ... as well as acting as a substrate for bacterial DNA ligases and a group of enzymes called sirtuins that use NAD+ to remove ...
C content of bacterial chromosomes by monitoring fluorescence intensity during DNA denaturation in a capillary tube". Int. J. ... "DNA-DNA hybridization determined in micro-wells using covalent attachment of DNA". Int. J. Syst. Evol. Microbiol. 50: 1095-1102 ... Euzeby, JP (1997). "List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet". Int. J. Syst. ... Palys, T; Nakamura LK; Cohan FM (1997). "Discovery and classification of ecological diversity in the bacterial world: the role ...
"A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity". Science. 337 (6096): 816-821. doi:10.1126/ ... acting as a programmable DNA binding protein, to cleave DNA at a site of interest.[1][2] ... Exposing various ectopic DNA loci to natural lncRNAs can help show the effects of lncRNAs on gene expression and chromatin ... and inability to distinguish lncRNA function from other confounding factors like cryptically encoded peptides or functional DNA ...
DNA vector, episomal, lasting expression, immunogenic Bacterial vector species (bacterial minicells can carry plasmids, siRNAs ... and/or chromatic or DNA modification.[7][8][9] In the context in which the phenomenon was first studied, small RNA was found to ... including repetitive DNA and transposons.[44] However, the biogenesis of piRNAs is also the least well understood.[45] piRNAs ... like DNA, is a double stranded series of nucleotides. If the mechanism didn't use dsRNAs, but only single strands, there would ...
Kreth, Jens; Merritt, J.; Qi, F. (August 2009). "Bacterial and Host Interactions of Oral Streptococci". DNA and Cell Biology. ... The bacterial equilibrium position varies at different stages of formation. Below is a summary of the bacteria that may be ... doi:10.1089/dna.2009.0868. Retrieved 2012-08-22. (Subscription required (help)). P D Marsh, D J Bradshaw. "Dental plaque as a ... Plaque is rich in species, given the fact that about 1000 different bacterial species have been recognised using modern ...
Other NAD-dependent enzymes include bacterial DNA ligases, which join two DNA ends by using NAD+ as a substrate to donate an ... Wilkinson A, Day J, Bowater R (2001). "Bacterial DNA ligases". Mol. Microbiol. 40 (6): 1241-8. doi:10.1046/j.1365-2958.2001. ... as well as acting as a substrate for bacterial DNA ligases and a group of enzymes called sirtuins that use NAD+ to remove ... "A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks". ...
The DNA sequence could be distinguished by the specific modulating effect of the four bases on the ionic current through the ... Loman, N.J.; Quick, J.; Simpson, J.T. (2015). "A complete bacterial genome assembled de novo using only nanopore sequencing ... His work led to a novel method of DNA sequencing and a more complete understanding of the role of membranes in the origin of ... The first publications appeared in 2015, one of which used the MinION to sequence E. coli DNA with 99.4% accuracy relative to ...
Jayaraman R (2008). "Bacterial persistence: some new insights into an old phenomenon" (PDF). J Biosci. 33 (5): 795-805. doi: ... As a result of the DNA damage, yeast persisters are also enriched for random genetic mutations that occurred prior to the ... Bacterial multidrug or antibiotic tolerance poses medically important challenges. It is largely responsible for the inability ... Yeast persisters are triggered in a small subset of unperturbed exponentially growing cells by spontaneously occurring DNA ...
Mitochondrial DNA resembles bacterial DNA. If bacteria triggers leukocytes, mitochondrial DNA may do the same. When confronted ... mitochondrial DNA is the leading cause of severe inflammation due to a massive amount of Mitochondrial DNA that leaks into the ... 2014). "Mitochondrial DNA neutrophil extracellular traps are formed after trauma and subsequent surgery". Journal of Critical ... Simple intestinal obstruction causes bacterial translocation in man. Arch Surg 1989; 124: 699-701. PMID 2730322 McIlroy DJ; et ...
Natural transformation is a common bacterial adaptation for DNA transfer that employs numerous bacterial gene products. For a ... 496-8. ISBN 0-387-24144-2. Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): ... The DNA-uptake process of naturally competent V. cholerae involves an extended competence-induced pilus and a DNA binding ... V. cholerae has been used in discoveries of many bacterial small RNAs. Using sRNA-Seq and Northern blot candidate sRNAs were ...
specifically identified bacterial DNA as the underlying component of the lysate that elicited the response. Then, in 1995 Krieg ... Bauer, S; Wagner, H (2002). "Bacterial CpG-DNA licenses TLR9". Current topics in microbiology and immunology. Current Topics in ... demonstrated that the CpG motif within bacterial DNA was responsible for the immunostimulatory effects and developed synthetic ... "CpG motifs in bacterial DNA trigger direct B-cell activation". Nature. 374 (6522): 546-9. doi:10.1038/374546a0. PMID 7700380. ...
Natural transformation is a bacterial adaptation for DNA transfer (HGT) that depends on the expression of numerous bacterial ... the process in which bacterial DNA is moved from one bacterium to another by a virus (a bacteriophage, or phage). Bacterial ... Chen I, Dubnau D (2004). "DNA uptake during bacterial transformation". Nat. Rev. Microbiol. 2 (3): 241-9. doi:10.1038/ ... The frequency of recombination is increased by DNA damage induced by UV-irradiation and by DNA damaging chemicals. The ups ...
"Entrez Gene: LANCL2 LanC lantibiotic synthetase component C-like 2 (bacterial)". Lu P, Hontecillas R, Philipson CW, Bassaganya- ... DNA Sequence. 12 (3): 161-6. doi:10.3109/10425170109080770. PMID 11762191. " ...
Chen I, Dubnau D. DNA uptake during bacterial transformation. Nat. Rev. Microbiol.. 2004, s. 241-9. DOI:10.1038/nrmicro844. ... Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA.. ... V roce 1972 Paul Berg vytvořil první molekuly rekombinantní DNA spojením DNA opičího viru SV40 a lambda viru.[5] V roce 1973 ... která pak může existovat jako extrachromozomální DNA nebo se začlenit do jejich genomu. Do živočišných buněk se DNA může vnášet ...
Wilkinson A, Day J, Bowater R «Bacterial DNA ligases». Mol. Microbiol., 40, 6, 2001, pàg. 1241-8. DOI: 10.1046/j.1365-2958.2001 ... d'un extrem del DNA. Aquest intermediari és seguidament atacat pel grup hidroxil 3' de l'altre extrem de DNA, formant un nou ... A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks». ... Chambon P, Weill JD, Mandel P «Nicotinamide mononucleotide activation of new DNA-dependent polyadenylic acid synthesizing ...
Quinolones inhibit the bacterial DNA gyrase or the topoisomerase IV enzyme, thereby inhibiting DNA replication and ... Castora, F. J.; Vissering, F. F.; Simpson, M. V. (September 1983). "The effect of bacterial DNA gyrase inhibitors on DNA ... Structure of bacterial DNA gyrase complexed with DNA and two ciprofloxacin molecules (green) ... Naphthyridone and quinolone classes of antibiotics prevent bacterial DNA replication by inhibition of DNA unwinding events, and ...
... self-replicating DNA molecules that are separate from the bacterial chromosome.[10] Plasmids can carry genes responsible for ... Transformation is a bacterial process for transferring DNA from one cell to another, and is apparently an adaptation for ... allows for efficient recombinational repair of DNA damage [13] and a greater range of genetic diversity by combining the DNA of ... Under stressful environmental conditions that cause DNA damage, some species of archaea aggregate and transfer DNA between ...
Lanka Erich, Wilkins Brian M (1995). "DNA Processing Reactions in Bacterial Conjugation." Annu. Rev. Biochem. 64: 141-69 Matson ... The nic site or nick region is found within the origin of transfer (oriT) site and is a key in starting bacterial conjugation.[ ... This single strand is eventually transferred to the recipient cell during the process of bacterial conjugation. Before this ... 1] A single strand of DNA, called the T-strand, is cut at nic by an enzyme called relaxase. ...
Natural bacterial transformation involves the transfer of DNA from one bacterium to another through the surrounding medium. ... The genome of S. pneumoniae is a closed, circular DNA structure that contains between 2.0 and 2.1 million base pairs depending ... Competence in S. pneumoniae is induced by DNA-damaging agents such as mitomycin C, fluoroquinolone antibiotics (norfloxacin, ... The ability of S. pneumoniae to repair the oxidative DNA damages in its genome, caused by this host defense, likely contributes ...
... that is necessary for transfer of the DNA that contains it from a bacterial host to recipient during bacterial conjugation. The ... "DNA Processing Reactions in Bacterial Conjugation." Annual Reviews in Biochemistry 64:141-169. ... Relaxase then moves in the 5' to 3' direction on the plasmid, unwinding the DNA in a helicase-like fashion, until it comes a ... "Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC." Nature Structural Biology Vol. 10 No. ...
... which depend on the presence of unmethylated CpG dinucleotides in the bacterial DNA. In contrast, mammalian DNA has a low ... vertebrate immune systems appear to have evolved a specific Toll-like receptor that distinguishes bacterial DNA from self-DNA. ... A Toll-like receptor recognizes bacterial DNA.. Hemmi H1, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino ... CpG DNA induces a strong T-helper-1-like inflammatory response. Accumulating evidence has revealed the therapeutic potential of ...
1992) Mammalian DNA polymerase beta can substitute for DNA polymerase I during DNA replication in Escherichia coli. J Biol Chem ... Optimization of DNA polymerase mutation rates during bacterial evolution. Ern Loh, Jesse J. Salk, and Lawrence A. Loeb ... Optimization of DNA polymerase mutation rates during bacterial evolution Message Subject (Your Name) has sent you a message ... 1990) The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. J Biol Chem 265:13878-13887. ...
A) Proposed model for colibactin DNA alkylation and formation of DNA adducts 1 and 2 (DNA = deoxyribonucleic acid; Ade = ... E) Bacterial load in the feces of mice colonized with pBelo (n = 3) or pks+ E. coli (n = 8) for 2 weeks. (F) EIC counts of DNA ... The human gut bacterial genotoxin colibactin alkylates DNA.. Wilson MR#1, Jiang Y#1, Villalta PW2, Stornetta A2, Boudreau PD1, ... ctDNA = calf-thymus DNA; PLE = pig liver esterase. (B) Chemical structures of diastereomeric DNA adducts 6 and 7 showing key 2D ...
However, the bacterial recombinational potential with DNA should be seen in the broader context of DNA exposure. Of the vast ... Ancient bacterial DNA is extremely difficult to authenticate (30). Therefore, to exclude modern DNA contamination, we instead ... E) Diagram of the ancient DNA experiment. Woolly mammoth DNA was used as donor DNA for natural transformation of the hisC::′ ... To confirm that the results with modern fragmented and damaged DNA also apply to ancient DNA, we recovered 43,000-y-old DNA. ...
Bacterial protein mimics DNA to sabotage cells defenses. American Society for Biochemistry and Molecular Biology ... Bacterial protein mimics DNA to sabotage cells defenses Study reveals details of Salmonella infections ... "These (bacterial proteins) function as a molecular pair of scissors, cutting up NF-kappaB transcription factors and thereby ... "So what I think this selectivity means is that (the bacterial proteins) are able to affect a particular arm of the immune ...
... the famous double helix together in a unique fashion which foils the standard repair mechanisms cells use to protect their DNA. ... Vanderbilt researchers unravel how bacterial toxin prevents DNA replication. *Download PDF Copy ... Tags: Antifungal, Bacteria, Cancer, Cell, Chemotherapy, Compound, DNA, DNA Replication, Enzyme, Gene, Helix, Inflammation, ... As a result, it stabilizes the DNA instead of destabilizing it, and it does so without distorting the DNA structure so NER ...
If you want to teach your old bacteria new tricks, youll have to do it directly by changing its DNA. Heres Tuur van Balen at ... If you want to teach your old bacteria new tricks, youll have to do it directly by changing its DNA. ...
Extracellular DNA Required for Bacterial Biofilm Formation. By Cynthia B. Whitchurch, Tim Tolker-Nielsen, Paula C. Ragas, John ... Extracellular DNA Required for Bacterial Biofilm Formation. By Cynthia B. Whitchurch, Tim Tolker-Nielsen, Paula C. Ragas, John ... Extracellular DNA Required for Bacterial Biofilm Formation Message Subject. (Your Name) has forwarded a page to you from ... which holds bacterial biofilms together, is a complex mixture of macromolecules including exopolysaccharides, proteins, and DNA ...
Demonstrates the DNA extraction process of freeze-dried E. coli cells. Cell walls are broken with a detergent, and the DNA is ... Bacterial DNA Extraction Kit: E. coli Class Kit Refill Item #154697 $44.95 ... Bacterial DNA Extraction Kit: E. coli. 2 Items *bvseo_sdk, java_sdk, bvseo-4.0.0 ... Bacterial DNA Extraction Kit: E. coli Class Kit Refill Item #154697 $44.95 ...
DNA/RNA tunnel of bacterial DNA dependent RNA polymerase (IPR021975). Short name: RNApol_Rpb2_rif ... This domain is part of the beta subunit of bacterial DNA dependent RNA polymerase. This domain is the binding site for the ... antibacterial drug rifampin (and its analogues) which blocks the DNA/RNA tunnel and prevents initiation of transcription. ...
Biospecimen Retention: Samples With DNA. Sample with bacterial DNA (and no human DNA will be studied) ... Bacterial Infection Diagnosis Using Blood DNA. The safety and scientific validity of this study is the responsibility of the ... The goal of the current study is to evaluate the diagnostic value of plasma detection of bacterial DNA in ICU patients with a ... Bacterial DNA Detection as a Diagnostic Tool of Infection in Critical Ill Patients With SIRS. ...
Buy and find information on the Bacterial Genomic DNA Miniprep Kit with 350 purifications for bacterial genomic DNA ... GenElute™ Bacterial Genomic DNA Kits sufficient for 350 purifications Synonym: Bacterial Genomic DNA, Gen Elute ... GenElute Bacterial Genomic DNA Kit Protocol Our GenElute™ Bacterial Genomic DNA Kit provides a simple and convenient way to ... The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative ...
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 468, 67 (2010). doi:10.1038/nature09523 ... Mechanism of foreign DNA selection in a bacterial adaptive immune system. Mol. Cell 46, 606 (2012). doi:10.1016/j.molcel. ... A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity Message Subject. (Your Name) has forwarded a ... A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. By Martin Jinek, Krzysztof Chylinski, Ines ...
They found that bacterial DNA was more likely to integrate in the genome in tumor samples than in normal, healthy somatic cells ... Bacterial DNA may integrate into the human genome more readily in tumors than in normal human tissue, scientists have found. ... Bacterial DNA May Integrate Into Human Genome More Readily in Tumor Tissue. ... The 3-D structure of the human genome, shown in a stretch of DNA inside a fractal globule.. Credit and Larger Version ...
Bacterial Genomic DNA Extraction - (Feb/16/2007 ). How do we make sure that it is only Genomic DNA and no PLASMID DNA that ... Excise the genomic DNA from the gel, extract the DNA by one of the old gel extraction protocol (do not use column, as the will ... A DNA prep which allows you to spool or hook the genomic DNA on a glass rod will allow you to wash small plasmid fragments, ... A different idea would be to gel purify your DNA. Assuming your plasmid is small (,7kb), you can gel purify your DNA on a low ...
The GenElute Bacterial Genomic Kit provides a simple and convenient technique to isolate high quality DNA from both Gram(-) and ... Figure 3. Purified genomic DNA was isolated from various bacterial species using the GenElute Bacterial Genomic DNA kit. A 1 µg ... Typical DNA Yields with the GenElute Bacterial Genomic DNA Kit.. Source. Type of Media. Amount of Overnight Culture. OD600 per ... The GenElute Bacterial Genomic DNA Kit contains all of the reagents needed to purify genomic DNA from Gram negative bacteria ( ...
However, our study also suggested that arsenated DNA (As-DNA) is still less stable than normal DNA when hydrolysis is ... Normal DNA has a backbone made of sugar and phosphate groups joined by phosphodiester linkages. Arsenic replaces the ... Now, scientists from the US and China say that arsenic substituted DNA may be more stable than first thought. ... Could Hydrolysis of Arsenic Substituted DNA be Prevented?: Protection Arises from Stacking Interactions. Jing Wang, Jiande Gu ...
For the extraction of protein and nucleic acids from bacterial cell cultures ... Easy-to-use, the AllPrep Bacterial DNA/RNA/Protein Kit isolates total nucleic acids and cellular proteins from bacterial ... Home − Shop − Sample Technologies − RNA − Total RNA − Allprep Bacterial DNA/RNA/Protein Kit ... The Allprep Bacterial DNA/RNA/Protein Kit is intended for molecular biology applications. This product is not intended for the ...
... substantial research efforts have been directed at developing bacterial sensing methods that are sensitive, specific, ... Bacterial detection plays an important role in protecting public health and safety, and thus, ... A Sensitive DNA Enzyme-Based Fluorescent Assay for Bacterial Detection. Sergio D. Aguirre 1,†. ... Aguirre, S.D.; Ali, M.M.; Salena, B.J.; Li, Y. A Sensitive DNA Enzyme-Based Fluorescent Assay for Bacterial Detection. ...
The common bacterial base modification N6-methyladenine (m6A) is involved in many pathways related to an organisms ability to ... Nanopore detection of bacterial DNA base modifications 13th April 2017 - BioRxiv. The common bacterial base modification N6- ... the ability to reliably characterize m6A presents an opportunity to further examine the role of methylation in bacterial ...
Computer artwork of rings of double-stranded DNA (deoxyribonucleic acid). Bacterial DNA is typically found in rings like this, ... Similar rings of DNA are also used in cloning techniques. - Stock Image C011/3887 ... Caption: Bacterial DNA. Computer artwork of rings of double-stranded DNA (deoxyribonucleic acid). Bacterial DNA is typically ... Similar rings of DNA are also used in cloning techniques.. Release details: Model release not required. Property release not ...
... are lethal events in organisms carrying DNA as their genome, which include bacteria ... DNA) double‐strand breaks,which are caused by many factors such as chemical treatments, radiations and, often, biological ... DNA without a χ sequence would be destroyed by extensive DNA degradation (h); DNA with χ would be repaired (j). Single‐strand ... Bacterial cells dying from chromosomal DNA double‐strand breaks made by a restriction enzyme. (a) Escherichia coli. cells ...
Bacteria also contain smaller circular DNA molecules called plasmids. ... Bacterial DNA - the role of plasmids. Like other organisms, bacteria use double-stranded DNA as their genetic material. However ... bacteria organise their DNA differently to more complex organisms. Bacterial DNA - a circular chromosome ... ... Find out how and why we use bacteria to improve our lives, and discover how the DNA revolution has led to new uses for bacteria ...
... substantial research efforts have been directed at developing bacterial sensing methods that are sensitive, specific, ... Bacterial detection plays an important role in protecting public health and safety, and thus, ...
Programmable DNA scissors: A double-RNA structure in the bacterial immune system has been discovered that directs Cas9 enzymes ... Programmable DNA scissors found for bacterial immune system. by Lynn Yarris, Lawrence Berkeley National Laboratory ... "Weve discovered the mechanism behind the RNA-guided cleavage of double-stranded DNA that is central to the bacterial acquired ... phys.org/news/2012-06-programmable-dna-scissors-bacterial-immune.html This document is subject to copyright. Apart from any ...
  • In the new work, researchers have essentially caught one step of this arms race in action, and they have shed light on the molecular mechanisms employed by a bacterial pathogen to survive in the face of its host plant's defenses. (bio-medicine.org)
  • These findings offer a molecular explanation for how exposure to plant resistance mechanisms can directly drive the evolution of new virulent forms of a bacterial pathogen. (bio-medicine.org)
  • Molzym announces CE IVD marking of their robotic microbial DNA isolation and direct PCR test, Micro-Dx™, for routine pathogen diagnosis. (bionity.com)
  • In this article, we reviewed strategies that have been used to improve and modulate the immune response induced by DNA vaccines, using as a model the intracellular bacterial pathogen Brucella abortus. (eurekaselect.com)
  • Sophie Leclercq, Jerome S. Harms and Sergio Costa Oliveira, " Enhanced Efficacy of DNA Vaccines Against an Intracellular Bacterial Pathogen by Genetic Adjuvants", Current Pharmaceutical Biotechnology (2003) 4: 99. (eurekaselect.com)
  • This novel fast broad-range 16S rDNA PCR/sequencing test had superior sensitivity compared to tissue Gram stain and culture for identifying underlying bacterial pathogen in both native and prosthetic valve endocarditis. (biomedcentral.com)
  • We are comparing commensal (innocuous) E. coli strains with the pathogen E. coli O157:H7, for which we have fabricated DNA microarrays, to identify factors that allow the pathogen to infect normally colonized hosts. (ou.edu)
  • ATCC offers genomic DNA from a variety of fungi, including Geomyces destructans , the pathogen responsible for White Nose Syndrome in bats. (atcc.org)
  • Genomes of various lytic and lysogenic phages have been shown to encode multi- and mono-specific orphan MTases that have the ability to confer protection from restriction endonucleases of their bacterial host(s). (asm.org)
  • In a paper published in Autoimmunity Reviews , the ARF team, under the guidance of Professor Trevor Marshall of Murdoch University, Western Australia, has explained how Homo sapiens must now be viewed as a superorganism in which a plethora of bacterial genomes-a metagenome-work in concert with our own. (mpkb.org)
  • The opportunity to use online computational tools in the context of bacterial genomes will also be of interest to teachers and their 16-18-year-old science and computing students. (wellcomegenomecampus.org)
  • The study attempted to assess the profile of elevated arsenic (As), cadmium (Cd) and mercury (Hg)-resistant bacterial community structures of sludge (S1, India), sludge and sediment (S2 and S3, Japan) and sediment (S4, Vietnam) samples by metagenomic-DNA fingerprinting using polymerase chain reaction-denaturing gradient gel electrophoresis ( PCR-DGGE) for monitoring and bioremediation of hazardous metal(loid) contamination in environment. (springer.com)
  • Complexity and heterogeneity of soil samples have often implied the inclusion of purification steps in conventional DNA extraction for polymerase chain reaction (PCR) assays. (e-eht.org)
  • With recent demonstrations of nanopore sequencing in Antarctica and onboard the International Space Station, the ability to reliably characterize m6A presents an opportunity to further examine the role of methylation in bacterial adaptation to extreme environments. (nanoporetech.com)
  • Methylation of DNA at CpG dinucleotides or inversion of the motif abolished this protection. (jimmunol.org)
  • Typical type I R-M systems consist of three subunits, the S (specificity subunit), M (methyltransferase), and R (restriction endonuclease) subunits, where the S subunit determines the target recognition specificity of the system, while the M and R subunits are required for methylation activity and DNA restriction, respectively ( 7 ). (asm.org)
  • DNA methylation provides a mechanism by which additional information is imparted to DNA, and such epigenetic information can alter the timing and targeting of cellular events ( 47 ). (asm.org)
  • In this review, we focus our attention on the roles of DNA methylation in regulating bacterial gene expression and virulence. (asm.org)
  • Although some background information about DNA methylation is presented, we refer the reader to excellent reviews on the subject ( 5 , 15 , 28 , 47 , 64 ). (asm.org)
  • DNA methylation occurs at the C-5 or N-4 positions of cytosine and at the N-6 position of adenine and is catalyzed by enzymes known as DNA methyltransferases (MTases) ( 57 , 59 ). (asm.org)
  • DNA methylation has historically been associated with DNA restriction-modification systems thought to be important in protecting cells from foreign DNAs such as transposons and viral DNAs ( 35 , 50 , 69 ). (asm.org)
  • A) Structural features of DNA adducts and detection by neutral loss monitoring. (nih.gov)
  • Bacterial detection plays an important role in protecting public health and safety, and thus, substantial research efforts have been directed at developing bacterial sensing methods that are sensitive, specific, inexpensive, and easy to use. (mdpi.com)
  • The optimized assay can achieve a detection limit of 1000 colony-forming units (CFU) without a culturing step and is able to detect 1 CFU following as short as 4 h of bacterial culturing in a growth medium. (mdpi.com)
  • Overall, our effort has led to the development of a highly sensitive and easy-to-use fluorescent bacterial detection assay that employs a catalytic DNA. (mdpi.com)
  • The demand for rapid and sensitive bacterial detection is continuously increasing due to the significant requirements of various applications. (rsc.org)
  • In this study, a terahertz (THz) biosensor based on rolling circle amplification (RCA) was developed for the isothermal detection of bacterial DNA. (rsc.org)
  • The proposed strategy not only represents a new method for the isothermal detection of the target bacterial DNA but also provides a general methodology for sensitive and specific DNA biosensing using THz spectroscopy. (rsc.org)
  • Depending on the methodology of DNA extraction, this phenomenon causes a shift in detection of microbial taxa in ecosystems and a possible misinterpretation of microbial interactions. (diva-portal.org)
  • The removal of human DNA and free-circulating microbial DNA ensures high detection specificity of exclusively DNA of intact cells. (bionity.com)
  • We have recently reported the detection of bacterial DNA in blood and ascitic fluid from patients with advanced cirrhosis, what we consider as molecular evidence of bacterial translocation. (bmj.com)
  • It is well-established that CRISPR systems can be transplanted into heterologous bacterial strains," she says. (phys.org)
  • We investigated the usefulness of a novel DNA fingerprinting technique, AFLP, which is based on the selective amplification of genomic restriction fragments by PCR, to differentiate bacterial strains at the subgeneric level. (microbiologyresearch.org)
  • In total, 147 bacterial strains were subjected to AFLP fingerprinting: 36 Xanthomonas strains, including 23 pathovars of Xanthomonas axonopodis and six pathovars of Xanthomonas vasicola, one strain of Stenotrophomonas, 90 genotypically characterized strains comprising all 14 hybridization groups currently described in the genus Aeromonas, and four strains of each of the genera Clostridium, Bacillus, Acinetobacter, Pseudomonas and Vibrio. (microbiologyresearch.org)
  • Genetic sequencing data is already available for vesicles from several bacterial strains, but it is not yet clear how the genetic makeup of vesicles differ from that of their parent cells, and which properties may characterize enriched genetic material. (frontiersin.org)
  • One of the most potent toxins known acts by welding the two strands of the famous double helix together in a unique fashion which foils the standard repair mechanisms cells use to protect their DNA. (news-medical.net)
  • In addition to these endogenous sources, ssDNA is also produced by several mechanisms of exogenous DNA uptake involved in lateral gene transfer, namely by conjugation, transformation and occasionally transduction. (prolekare.cz)
  • Several bacterial mechanisms have been proven to be associated with the secretion of EVs, such as biofilm formation, nutrient acquisition and secretion of virulence determinants into host cells ( Kulp and Kuehn, 2010 ). (frontiersin.org)
  • Our study, together with the reported involvement of the mammalian PolIV homolog, Polκ, in similar activity, indicates that Y-family DNA polymerases from the DinB branch can be added to the list of evolutionarily conserved molecular mechanisms that counteract cytotoxic effects of DNA alkylation. (genetics.org)
  • Here we combine an untargeted DNA adductomics approach with chemical synthesis to identify and characterize a covalent DNA modification from human cell lines treated with colibactin-producing E. coli Our data establish that colibactin alkylates DNA with an unusual electrophilic cyclopropane. (nih.gov)
  • We show that this metabolite is formed in mice colonized by colibactin-producing E. coli and is likely derived from an initially formed, unstable colibactin-DNA adduct. (nih.gov)
  • pks + E. coli synthesize cyclopropane-containing metabolites that may alkylate DNA. (nih.gov)
  • High-resolution accurate mass (HRAM) LC-MS 3 DNA adductomic analysis identifies DNA adducts in HeLa cells and mice exposed to pks + E. coli . (nih.gov)
  • B) Full scan extracted ion chromatogram (EIC) of DNA adducts 1 and 2 ( m/z 540.1772) in HeLa cells exposed to colibactin-producing E. coli and negative controls (HeLa cells exposed to non-colibactin producing pBeloBAC E. coli, HeLa cells alone, or when no DNA was present). (nih.gov)
  • Multiple routes in the processing of DNA double‐strand breaks in E. coli . (els.net)
  • In vitro studies of reconstituted E. coli replisomes have attributed this remarkable processivity to the high stability of the replisome once assembled on DNA. (elifesciences.org)
  • By examining replisomes in live E. coli with fluorescence microscopy, we found that the Pol III* subassembly frequently disengages from the replisome during DNA synthesis and exchanges with free copies from solution. (elifesciences.org)
  • At the core of the E. coli replisome is the replicative helicase, DnaB, which encircles the lagging strand template and unwinds parental DNA. (elifesciences.org)
  • In this study we have assessed the structural differences between E. coli DNA and PO-ODN, which may explain the high activity of bacterial DNA on macrophages. (semanticscholar.org)
  • Soft X-ray tomography was used to visualize bacterial chromatin (in yellow) in wild type and invasive E. coli cells, shown in the bottom row. (scienceconnected.org)
  • After a DNA fragment coding for the production of msDNA in E. coli was discovered, it was conjectured that bacteriophages might have been responsible for the introduction of the RT gene into E. coli. (wikipedia.org)
  • Among these architectural elements is the structure of DNA itself, its variable nature at a topological rather than just at a base-sequence level and its ability to play an active (as well as a passive) part in the gene regulation process. (clinsci.org)
  • Ratios for each bacterial phylum (target gene) relative to all 16S rDNA (reference gene) were calculated in reference to a stool sample that was immediately frozen at −80°C (reference sample). (bmj.com)
  • The presence of anti-SOS factors in some conjugative plasmids, such as the psiB gene of R64 drd and R100-1 , suggests that conjugative DNA transfer can induce SOS. (prolekare.cz)
  • Non-viral gene transfer using plasmid DNA (pDNA) is generally acknowledged as safe and non-immunogenic compared with the use of viral vectors. (cfgenetherapy.org.uk)
  • Using pyrosequencing of the 16S rRNA gene and ITS2 region amplicons, we analysed the bacterial and fungal gut communities of the honey bee as affected by the host social status. (nature.com)
  • PICRUSt analysis revealed significant differences in enriched gene clusters of the bacterial gut communities of the nurse and foraging bees, suggesting that different host social status might induce changes in the gut microbiota, and, that consequently, gut microbial community shifts to adapt to the gut environment. (nature.com)
  • For 16S rRNA gene amplicon-based community profiling, DNA was extracted from the sediment and filters and the bacterial V3-V4 regions were amplified and sequenced using a MiSeq instrument (Illumina). (nature.com)
  • However, past research has shown that DNA supercoiling can have a profound effect on gene expression and regulation. (scienceconnected.org)
  • Charged residues in the H-NS linker drive DNA binding and gene silencing in single cells. (mechanobio.info)
  • H-NS binding to DNA leads to gene silencing. (mechanobio.info)
  • When the H-NS linker is removed or mutated, DNA binding is reduced and its gene silencing function is lost. (mechanobio.info)
  • Is there any specific component in the protocol that does this job of alleviating Plasmid DNA during Genomic DNA extraction? (protocol-online.org)
  • These kits do not utilize toxic agents, such as phenol or chloroform for genomic DNA extraction. (gbiosciences.com)
  • A DNA extraction that comprises the DNA of all available taxa in an ecosystem is an essential step in population analysis, especially for next generation sequencing applications. (diva-portal.org)
  • Many nanoparticles as well as naturally occurring clay minerals contain charged surfaces or edges that capture negatively charged DNA molecules after cell lysis within DNA extraction. (diva-portal.org)
  • Altogether, 13 different methods of commercially available DNA extraction kits provided by seven companies as well as the classical phenol-chloroform DNA extraction were compared. (diva-portal.org)
  • In this paper, we describe how to combine several DNA extraction methods for the investigation of microbial community structures in clay. (diva-portal.org)
  • SepsiTest-UMD CE IVD includes all reagents and consumables necessary for DNA extraction and broad range PCR or Real-Time PCR analysis as well as primers for Sanger-sequencing. (bionity.com)
  • 1 gFOBT cards were found to be an easy to use option for stool collection and gained results comparable to fresh stool, even when cards were stored for up to 3 years at ambient temperature before DNA extraction. (bmj.com)
  • To develop and test new DNA extraction procedures, it would be helpful to determine their efficiencies. (biomedcentral.com)
  • The acid/HPLC method is a new tool to determine DNA extraction efficiencies and should aid in the development of improved protocols for releasing DNA from a broad range of microorganisms. (biomedcentral.com)
  • To calculate the efficiency of extraction, the amount of DNA recovered and the total amount of DNA in the original sample must both be known. (biomedcentral.com)
  • Two 30-40 cm cores were used for bacterial community analysis and one for porewater extraction and analysis. (nature.com)
  • Bead beating and centrifugation was used as the baseline (without purification) method for DNA extraction. (e-eht.org)
  • Its performance was compared with that of conventional DNA extraction kit (with purification). (e-eht.org)
  • Using lysis conditions at 3000 rpm for 3 minutes with 0.1 mm glass beads, centrifugation time of 10 minutes and 1:10 dilution ratio, the baseline method outperformed conventional DNA extraction on cell seeded sand samples. (e-eht.org)
  • It was concluded that DNA extraction method without purification is suitable for soil samples that have less than 10 μg/g of humic acids, less than 70% clay content and less than 0.01% Mg ion content. (e-eht.org)
  • These substances can remain in genomic DNA preparations via coprecipitation during DNA extraction. (e-eht.org)
  • Today's DNA extraction kit for soil consists of multiple process steps as well as the use of multiple reagents and is suitable even for the most delicate sample. (e-eht.org)
  • In this regard, the DNA purification step during DNA extraction has begun to manifest itself as a painful bottleneck. (e-eht.org)
  • A large body of data has also emerged implicating bacterial translocation in the pathogenesis of AH. (bmj.com)
  • Bacterial DNA containing unmethylated CpG motifs activates mammalian lymphocytes and macrophages to produce cytokines and polyclonal Ig. (jimmunol.org)
  • Here, we show that bacterial DNA, as well as synthetic oligonucleotides containing CpG motifs, induce protection against large lethal doses of Francisella tularensis live vaccine strain (LVS) and Listeria monocytogenes . (jimmunol.org)
  • Recent studies indicate that the mammalian immune system is stimulated by DNA containing six base pair motifs consisting of an unmethylated CpG dinucleotide flanked by two 5′-purines and two 3′-pyrimidines (reviewed in Refs. (jimmunol.org)
  • Recognizing that the cell types and cytokines important in an early protective immune response to LVS and L. monocytogenes were the same as those stimulated by bacterial DNA, we hypothesized that host recognition of bacterial DNA containing CpG motifs contributes significantly to the stimulation of innate protective immunity. (jimmunol.org)
  • CpG motifs that frequently appear in bacterial DNA are recognized by mammalian immune system as danger signal to the host and induce immune responses. (nii.ac.jp)
  • Bacterial DNA or oligonucleotides containing unmethylated CpG motifs can minimize lipopolysaccharide-induced inflammation in the lower respiratory tract through an IL-12-dependent pathway. (semanticscholar.org)
  • To determine whether the systemic immune activation by CpG DNA could alter airway inflammation, we pretreated mice with either i.v. bacterial DNA (bDNA) or oligonucleotides with or without CpG motifs, exposed these mice to LPS by inhalation, and measured the inflammatory response systemically and in the lung immediately following LPS inhalation. (semanticscholar.org)
  • Cyclosporin A enhances IL-12 production by CpG motifs in bacterial DNA and synthetic oligodeoxynucleotides. (semanticscholar.org)
  • Equal proportions of DNA were resolved on a 1%, 1X TBE agarose gel. (sigmaaldrich.com)
  • A 1 µg aliquot of DNA from each respective bacterial sample was resolved on a 1% agarose gel in 0.5X TBE at 150 volts for 16 hours using a BioRad CHEF DRII system. (sigmaaldrich.com)
  • Agarose electrophoresis can be used to visualize the genomic DNA on an agarose gel. (gbiosciences.com)
  • Genomic DNA (5% of total purified DNA) was analyzed on a 1% agarose gel to demonstrate yield and quality of the DNA. (omegabiotek.com)
  • A) One per cent agarose gel showing DNA fragment sizes of DNA isolated from faecal immunochemical test (FIT) tubes (OC-sensor) and guaiac faecal occult blood test (gFOBT) cards (Hemoccult II). (bmj.com)
  • The source of this DNA is unclear, but it is presumably derived from membrane vesicles rather than cell lysis as we saw no evidence of the latter during biofilm formation. (sciencemag.org)
  • However, under extreme circumstances exhaustive exercise in such environments can result in severe endotoxaemia (the translocation of bacterial lipopolysaccharide (LPS) into the central circulation) [ 8 , 9 ], through intestinal injury and changes in permeability, which is thought to be associated with acute inflammation, sepsis, shock and organ failure that in rare cases may be fatal. (springer.com)
  • Thus, vertebrate immune systems appear to have evolved a specific Toll-like receptor that distinguishes bacterial DNA from self-DNA. (nih.gov)
  • Formation of these sessile communities and their inherent resistance to antibiotics and host immune attack are at the root of many persistent and chronic bacterial infections ( 1 ), including those caused by Pseudomonas aeruginosa , which has been intensively studied as a model for biofilm formation ( 2 , 3 ). (sciencemag.org)
  • Much of the tissue damage associated with P. aeruginosa infections of the cystic fibrosis (CF) lung epithelia is due to inflammatory responses of the host immune system, which may include responses to bacterial DNA ( 9 ). (sciencemag.org)
  • However, the bacterial determinants that stimulate either inflammatory or lymphocyte-dependent innate immune responses are poorly understood. (jimmunol.org)
  • Several studies have shown the immunogenic role of bacterial DNA in vitro, and we hypothesised that the presence of bacterial DNA could activate the type I immune response in peritoneal macrophages from these patients, leading to greater cytokine synthesis (interleukin (IL)-2 and IL-12, tumour necrosis factor α, and interferon γ) and effector molecules such as nitric oxide. (bmj.com)
  • Mammalian DNA suppresses CpG-induced immune responses. (nii.ac.jp)
  • CpG DNA, a novel immune enhancer for systemic and mucosal immunization with influenza virus. (semanticscholar.org)
  • I wonder which protocol should I follow, since I am not working with bacterial suspensions proper, and in the other hand I do not want to miss gram positives due to lysis failure. (protocol-online.org)
  • Purified DNA can be directly used in downstream applications without the need for further purification. (omegabiotek.com)
  • Procedures for the Purification of Bacterial Artificial Chromosome (BAC) DNA. (prinsep.com)
  • We have developed a spin column miniprep protocol for the purification of BAC DNA. (prinsep.com)
  • Therefore the necessity of DNA purification was re-visited and investigated for a variety of environmental soil samples that contained various amounts of PCR inhibitors. (e-eht.org)
  • The necessity criteria for DNA purification were established with environmental soil samples. (e-eht.org)
  • In order to circumvent potential inhibition by removing the above-mentioned substances from soil samples, subsequent DNA purification became necessary [ 10 , 13 - 17 ]. (e-eht.org)
  • Unfortunately the DNA purification process is extremely time-consuming (i.e., hours-days) and labor-intensive. (e-eht.org)
  • In addition, a significant portion of DNA is usually lost during the DNA purification process. (e-eht.org)
  • In other words, lower recovery yield, degradation, and damage of DNA are common with the extensive DNA purification processes [ 6 , 15 ]. (e-eht.org)
  • The results are discussed in light of what is known about the preservation of microbial DNA at the Iceman's site and of previous parasitological studies performed on the Iceman himself and on human coprolites. (calpoly.edu)
  • Here we describe an improved method for obtaining both phage and microbial DNA from a single skin or wound swab, characterize the yield of DNA in model samples, and demonstrate the utility of this approach with samples collected from a wound clinic. (springer.com)
  • DNA-based testing is becoming the preferred method both for identifying microorganisms and for characterizing microbial communities. (biomedcentral.com)
  • The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. (sigmaaldrich.com)
  • Can arsenic bind to bacterial DNA? (rsc.org)
  • The helicase then begins running along the strand and breaking the hydrogen bounds that bind it to the second strand, allowing each to become a substrate that can replicate a complete DNA molecule. (cuny.edu)
  • Moreover, the free C-terminal domain can rapidly decondense ParB networks independently of its ability to bind DNA. (csic.es)
  • DNA-NPs are able to bind to the eye and can be used as carrier platforms for the enhanced delivery of eye medication. (arvojournals.org)
  • It is hypothesized that DNA stretching by DnaA bound to the origin promotes strand separation which allows more DnaA to bind to the unwound region. (wikipedia.org)
  • As a result, it stabilizes the DNA instead of destabilizing it, and it does so without distorting the DNA structure so NER enzymes can't find it. (news-medical.net)
  • Type II DNA methyltransferases (MTases) are enzymes found ubiquitously in the prokaryotic world, where they play important roles in several cellular processes, such as host protection and epigenetic regulation. (asm.org)
  • Here we show that cellular response to CpG DNA is mediated by a Toll-like receptor, TLR9. (nih.gov)
  • These methylases participate in cellular regulatory events, including those that control bacterial virulence, which are the primary focus of this review. (asm.org)
  • This study reveals extensive evidence for the applicability of AFLP in bacterial taxonomy through comparison of the newly obtained data with results previously obtained by well-established genotypic and chemotaxonomic methods such as DNA-DNA hybridization and cellular fatty acid analysis. (microbiologyresearch.org)
  • Indirect methods for estimating cellular DNA content are based on microscopic cell counts or colony counts. (biomedcentral.com)
  • Bacterial DNA is typically found in rings like this, which are known as plasmids. (sciencephoto.com)
  • Bacterial and archaeon microbes face a never-ending onslaught from viruses and invading circles of nucleic acid known as plasmids. (phys.org)
  • TLR9-/- mice showed resistance to the lethal effect of CpG DNA without any elevation of serum pro-inflammatory cytokine levels. (nih.gov)
  • Inducible nitric oxide synthase, nitric oxide levels, and cytokine production were measured by immunoenzymometric assays in basal and harvested conditions according to the presence/absence of bacterial DNA. (bmj.com)
  • The presence of bacterial DNA in patients with decompensated cirrhosis is associated with marked activation of peritoneal macrophages, as evidenced by nitric oxide synthesising ability, together with enhanced cytokine production. (bmj.com)
  • Cytokine induction by a bacterial DNA-specific modified base. (semanticscholar.org)
  • CpG DNA induces a strong T-helper-1-like inflammatory response. (nih.gov)
  • In this manuscript, we show that conjugative transfer of ssDNA induces the bacterial SOS stress response, unless an anti-SOS factor is present to alleviate this response. (prolekare.cz)
  • Bifidobacteria DNA induces murine macrophages activation in vitro. (semanticscholar.org)
  • CpG DNA induces sustained IL-12 expression in vivo and resistance to Listeria monocytogenes challenge. (semanticscholar.org)
  • The Repli-g Advanced DNA Single Cell Kit is a perfect choice especially for small amounts of bacterial starting material because the reagents are decontaminated for any residual DNA, reducing possible artefacts. (qiagen.com)
  • Normally, the DNA strands that we used in our experiments separate when they are heated to about 40 degrees [Celsius] but, with YTM added, they don't come apart until 85 degrees. (news-medical.net)
  • This work has produced DNA strands containing regions of base pair mismatching and with terminal three-way junctions. (bl.uk)
  • A method to create longer DNA strands with three-way junctions at the termini has also been developed. (bl.uk)
  • Single strands of DNA were produced that could be annealed to produce terminal three-way junctions. (bl.uk)
  • DNA is made up of two strands that twist together to form a double helix. (elifesciences.org)
  • These strands need to be separated so they can be used as templates to make new DNA strands. (elifesciences.org)
  • An enzyme called DNA helicase is responsible for separating the two DNA strands and another enzyme makes the new DNA. (elifesciences.org)
  • Opening of the helicase enables entry of one of the DNA molecule's two strands. (cuny.edu)
  • As bacterial homologous recombination DNA degradation by RecBCD exonuclease/helicase from the double‐strand break (g) is attenuated at χ sequence. (els.net)
  • The potential of DNA structures in the field of materials science is hampered by current approaches to augmentation. (bl.uk)
  • This thesis will discuss the patterning of DNA structures with RecA. (bl.uk)
  • Two further DNA structures were produced on which patterning did not prove possible. (bl.uk)
  • These phages can transfer DNA from one bacterium to another through a process known as genetic transduction. (sciencenewsdaily.org)
  • During this process the original cell must copy its DNA so each new cell inherits a full set of genetic material. (elifesciences.org)
  • The era of molecular genetic has given a new breath for vaccine development with the achievement of the "Third Generation of Vaccines": the DNA vaccine. (eurekaselect.com)
  • Central to many bacterial WGS bioinformatic pipelines is the identification of genetic variants. (biomedcentral.com)
  • We found that PolIV performs an error-free bypass of DNA damage that accumulates in the alkA tag genetic background. (genetics.org)
  • When the amount of cytotoxic alkylating DNA lesions is increased by the treatment with chemical alkylating agents, PolIV is required for survival in an alkA tag -proficient genetic background as well. (genetics.org)
  • During studies of alginate biosynthesis in P. aeruginosa , we discovered that the majority of the extracellular material that reacted in the carbazole colorimetric assay was not exopolysaccharide but DNA [as determined by its peak absorbance at 260 nm, by electrophoretic display, and by its deoxyribonuclease (DNase) but not ribonuclease sensitivity] and therefore hypothesized that this DNA may play a functional role in P. aeruginosa biofilms. (sciencemag.org)
  • Using a tube ring assay ( 2 ), we found that addition of DNase I to the culture medium strongly inhibited biofilm formation (Web fig. 1A) ( 7 ), although not bacterial growth per se. (sciencemag.org)
  • Our results showed that 0.12 fmol of synthetic bacterial DNA and 0.05 ng μL −1 of genomic DNA could be effectively detected using this assay. (rsc.org)
  • 5 Supporting this hypothesis, in a previous work we detected the presence of bacterial DNA (bactDNA) fragments simultaneously in blood and AF in as many as 32% of patients with advanced cirrhosis and sterile non-neutrocytic AF, 6 a fact that we interpret as molecular evidence of BT. (bmj.com)
  • The presence of bacterial β-glucuronidase is also suggested. (hku.hk)
  • From the 12 gFOBT cards, seven had considerable medium to good quality DNA (fragment sizes between 2 and 12 kbp). (bmj.com)
  • Doudna is one of two corresponding authors of a paper in the journal Science describing this work titled "A programmable dual RNA-guided DNA endonuclease in adaptive bacterial immunity. (phys.org)
  • CpG DNA is a potent enhancer of specific immunity in mice immunized with recombinant hepatitis B surface antigen. (semanticscholar.org)