3 beta,12 beta,14-Trihydroxy-5 beta-card-20(22)-enolide. A cardenolide which is the aglycon of digoxin. Can be obtained by hydrolysis of digoxin or from Digitalis orientalis L. and Digitalis lanata Ehrh.
DNA probes specific for the identification of human papilloma virus.
A cardiotonic glycoside obtained mainly from Digitalis lanata; it consists of three sugars and the aglycone DIGOXIGENIN. Digoxin has positive inotropic and negative chronotropic activity. It is used to control ventricular rate in ATRIAL FIBRILLATION and in the management of congestive heart failure with atrial fibrillation. Its use in congestive heart failure and sinus rhythm is less certain. The margin between toxic and therapeutic doses is small. (From Martindale, The Extra Pharmacopoeia, 30th ed, p666)
Cyclopentanophenanthrenes with a 5- or 6-membered lactone ring attached at the 17-position and SUGARS attached at the 3-position. Plants they come from have long been used in congestive heart failure. They increase the force of cardiac contraction without significantly affecting other parameters, but are very toxic at larger doses. Their mechanism of action usually involves inhibition of the NA(+)-K(+)-EXCHANGING ATPASE and they are often used in cell biological studies for that purpose.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Tumor or cancer of the MALE GENITALIA.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.

In situ hybridization for the detection and localization of swine Chlamydia trachomatis. (1/205)

Gnotobiotic piglets were inoculated intralaryngeally with swine Chlamydia trachomatis strain R33 or orally with swine C. trachmatis strain R27. Archived formalin-fixed, paraffin-embedded tissues from piglets euthanatized 4-7 days postinoculation were examined by in situ hybridization for C. trachomatis nucleic acid using a nonradioactive digoxigenin-labeled DNA probes that targeted specific ribosomal RNA or omp1 mRNA molecules of the swine C. trachomatis strains. Positive hybridization signals were detected in bronchial epithelial cells, bronchiolar epithelial cells, pneumocytes, alveolar and interstitial macrophages, and jejunal and ileal enterocytes. Chlamydia-infected cells had a strong signal that was confined to the intracytoplasmic inclusions. Positive hybridization signals were not detected in tissue sections from an uninfected control piglet or in C. psittaci-infected sheep placenta. The morphology of host cells was preserved despite the relatively high temperature required in parts of the incubation procedure. The data indicate that in situ hybridization can be used to detect swine C. trachomatis in formalin-fixed, paraffin-embedded tissue specimens.  (+info)

Analysis of hepatitis G virus (HGV) RNA, antibody to HGV envelope protein, and risk factors for blood donors coinfected with HGV and hepatitis C virus. (2/205)

Serologic, biochemical, and molecular analyses were used to study hepatitis G virus (HGV), antibody to the HGV envelope protein (anti-E2), risk factors, clinical significance, and the impact of HGV on coexistent hepatitis C virus (HCV). Among 329 donors with confirmed HCV infection, 12% were HGV RNA-positive and 44% were anti-E2-positive (total exposure, 56%). HGV RNA and anti-E2 were mutually exclusive except in 9 donors (1.5%); 8 of 9 subsequently lost HGV RNA but anti-E2 persisted. HGV had little impact on alanine aminotransferase, aspartate aminotransferase, or gamma-glutamyl transpeptidase in donors with HGV infection alone or those coinfected with HCV. A multivariate analysis showed that intravenous drug abuse was the leading risk factor for HGV transmission, followed by blood transfusion, snorting cocaine, imprisonment, and a history of sexually transmitted diseases. In summary, HGV and HCV infections were frequently associated and shared common parenteral risk factors; HGV did not appear to cause hepatitis or to worsen the course of coexistent hepatitis C.  (+info)

In vitro scanning saturation mutagenesis of all the specificity determining residues in an antibody binding site. (3/205)

For the first time, each specificity determining residue (SDR) in the binding site of an antibody has been replaced with every other possible single amino acid substitution, and the resulting mutants analyzed for binding affinity and specificity. The studies were conducted on a variant of the 26-10 antidigoxin single chain Fv (scFv) using in vitro scanning saturation mutagenesis, a new process that allows the high throughput production and characterization of antibody mutants [Burks,E.A., Chen,G., Georgiou,G. and Iverson,B.L. (1997) Proc. Natl Acad. Sci. USA, 94, 412-417]. Single amino acid mutants of 26-10 scFv were identified that modulated specificity in dramatic fashion. The overall plasticity of the antibody binding site with respect to amino acid replacement was also evaluated, revealing that 86% of all mutants retained measurable binding activity. Finally, by analyzing the physical properties of amino acid substitutions with respect to their effect on hapten binding, conclusions were drawn regarding the functional role played by the wild-type residue at each SDR position. The reported results highlight the value of in vitro scanning saturation mutagenesis for engineering antibody binding specificity, for evaluating the plasticity of proteins, and for comprehensive structure-function studies and analysis.  (+info)

Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients. (4/205)

The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products was confirmed by Southern blot hybridisation to a digoxigenin-labelled H. pylori probe. Further confirmation of product identity was obtained by DNA sequencing of a proportion of the amplified products. The assay was demonstrated to be specific for H. pylori with a lower limit of detection of 100 fg of bacterial genomic DNA. In all, 73 samples from 29 patients were analysed, of which 24 (33%) were H. pylori-positive by PCR; the proportion of patients carrying H. pylori in at least one sampled site was 38% (11 of 29). This is the first study to demonstrate the presence of H. pylori in the subgingival plaque of patients with adult periodontitis and indicates that, in this patient group at least, subgingival plaque may be a reservoir for H. pylori infection.  (+info)

Evidence for ovarian granulosa stem cells: telomerase activity and localization of the telomerase ribonucleic acid component in bovine ovarian follicles. (5/205)

We have previously postulated that granulosa cells of developing follicles arise from a population of stem cells. Stem cells and cancer cells can divide indefinitely partly because they express telomerase. Telomerase is a ribonucleoprotein enzyme that repairs the ends of telomeres that otherwise shorten progressively upon each successive cell division. In this study we carried out cell cycle analyses and examined telomerase expression to examine our hypothesis. Preantral (60-100 microm) and small (1 mm) follicles, as well as granulosa cells from medium-sized (3 mm) and large (6-8 mm) follicles, were isolated. Cell cycle analyses and expression of Ki-67, a cell cycle-related protein, were undertaken on follicles of each size (n = 3) by flow cytometry; 12% to 16% of granulosa cells in all follicles were in the S phase, and less than 2% were in the G(2)/M phase. Telomerase activity (n = 3) was highest in the small preantral follicles, declining at the 1-mm stage and even further at the 3-mm stage. In situ hybridization histochemistry was carried out on bovine ovaries, and telomerase RNA was detected in the granulosa cells of growing follicles but not primordial follicles. Two major patterns of staining were observed in the membrana granulosa of antral follicles: staining in the middle and antral layers, and staining in the middle and basal layers. No staining was detected in oocytes. Our results strongly support our hypothesis that granulosa cells arise from a population of stem cells.  (+info)

Detection and localization of Mycoplasma hyopneumoniae DNA in lungs from naturally infected pigs by in situ hybridization using a digoxigenin-labeled probe. (6/205)

Mycoplasma hyopneumoniae DNA was detected in 20 naturally infected pigs by in situ hybridization using a nonradioactive digoxigenin-labeled DNA probe. A 520-base-pair DNA probe targeting a reiterative sequence of the M. hyopneumoniae genome was generated by the polymerase chain reaction. All 20 pigs infected with M. hyopneumoniae had distinct and positive hybridization signals without background staining. A strong hybridization signal was detected mainly in the luminal surface of bronchial and bronchiolar lining epithelial cells, whereas no hybridization signal was seen in the cytoplasm of bronchial and bronchiolar lining epithelial cells. When hybridization signal was detected in the luminal surface of bronchial and bronchiolar lining epithelial cells, a given bronchus or bronchiole had peribronchiolar lymphoid hyperplastic tissues. Hybridization signals were not seen in the peribronchiolar lymphoid hyperplastic tissues. A less intense signal was detected in the interstitial and alveolar macrophages randomly scattered in the thickened alveolar septa and spaces. Hybridization signal was rarely detected in the type I pneumocytes. The in situ hybridization technique developed in this study was useful for detection of M. hyopneumoniae nucleic acids in tissues taken from naturally infected piglets and may be a valuable technique for studying the pathogenesis of M. hyopneumoniae infection.  (+info)

Nonisotopic detection of Loma salmonae (microspora) in rainbow trout (Oncorhynchus mykiss) gills by in situ hybridization. (7/205)

Loma salmonae, a microsporidian parasite of salmonids of the genus Oncorhynchus, is a significant cause of economic loss in pen-reared chinook salmon (O. tschawytscha). Final stages of L. salmonae infections are easily recognized by the xenomas that form in the gills during sporogony. However, early prexenoma stages of infection (3 weeks or less after infection) are difficult to detect on histologic slides. An L. salmonae-specific single-stranded DNA probe labeled with digoxigenin was used to detect these prexenoma stages of L salmonae by in situ hybridization in experimentally infected rainbow trout. This method allows detection of the parasite in the gills only 2 weeks after infection, providing a sensitive and specific way of detecting L. salmonae during the early stages of infection.  (+info)

In situ distribution of hepatitis C virus replicative-intermediate RNA in hepatic tissue and its correlation with liver disease. (8/205)

Liver failure from chronic hepatitis C is the leading indication for liver transplantation in the United States. However, the pathogenesis of liver injury resulting from chronic hepatitis C virus (HCV) infection is not well understood. To examine the relationship between HCV replication in liver tissue and hepatocellular injury, a strand-specific in situ hybridization procedure was developed. The sensitivity and specificity of digoxigenin-labeled riboprobes were optimized by analyzing Northern blots and cell lines expressing HCV RNAs. For the current study, both genomic (sense) and replicative-intermediate (antisense) HCV RNAs were detected and quantified in 8 of 8 liver tissue specimens from infected patients versus 0 of 11 liver tissue specimens from noninfected controls. The distribution pattern for HCV replicative-intermediate RNA in liver was different from that for HCV genomic RNA. HCV genomic RNA was variably distributed throughout infected livers and was located primarily in the cytoplasm of hepatocytes, with some signal in fibroblasts and/or macrophages in the surrounding fibroconnective tissue. However, HCV replicative-intermediate RNA showed a more focal pattern of distribution and was exclusively localized in the cytoplasm of hepatocytes. There was no significant relationship between the distribution pattern for HCV genomic RNA and any indices of hepatocellular injury. However, a highly significant correlation was observed between the percentage of cells staining positive for replicative-intermediate RNA and the degree of hepatic inflammatory activity (P, < 0.0001). Furthermore, the ratio of cells staining positive for HCV replicative-intermediate versus genomic RNA correlated with the histological severity of liver injury (P, 0. 0065), supporting the hypothesis that active replication of HCV in liver tissue may be a significant determinant of hepatocellular injury.  (+info)

Digoxigenin is a steroidal glycoside compound that is derived from the digitalis plant, which includes foxglove species. This compound is known for its cardiotonic properties and has been used in the treatment of various heart conditions, such as congestive heart failure and atrial arrhythmias.

In a medical or scientific context, digoxigenin is often used in research and diagnostic applications due to its ability to bind to specific antibodies or other molecules. This binding property makes it useful for techniques like immunohistochemistry, where it can be used to label and visualize specific proteins or structures within cells or tissues.

It's important to note that digoxigenin itself is not a medication or treatment, but rather a component derived from a plant that has been used in the development of certain medications and research tools.

DNA probes for HPV (Human Papillomavirus) are specific DNA sequences that are used in diagnostic tests to detect and identify the presence of HPV DNA in a sample. HPV is a viral infection that can cause various types of cancer, including cervical, anal, and oropharyngeal cancers.

DNA probes for HPV work by binding to complementary sequences of HPV DNA in the sample. This binding can be detected and measured using various methods, such as hybridization, amplification, or labeling techniques. The use of DNA probes for HPV can help identify the specific type of HPV that is present in a sample, which can inform clinical management and treatment decisions.

It's important to note that not all HPV infections lead to cancer, and most HPV infections resolve on their own without causing any harm. However, certain high-risk types of HPV are more strongly associated with an increased risk of developing cancer, so identifying the presence and type of HPV infection can be useful for monitoring and managing patients who may be at higher risk.

Digoxin is a medication that belongs to a class of drugs called cardiac glycosides. It is used to treat various heart conditions, such as heart failure and atrial fibrillation, by helping the heart beat stronger and more regularly. Digoxin works by inhibiting the sodium-potassium pump in heart muscle cells, which leads to an increase in intracellular calcium and a strengthening of heart contractions. It is important to monitor digoxin levels closely, as too much can lead to toxicity and serious side effects.

Cardiac glycosides are a group of naturally occurring compounds that have a toxic effect on the heart. They are found in certain plants, including foxglove and lily of the valley, as well as in some toads and beetles. The most well-known cardiac glycoside is digoxin, which is derived from the foxglove plant and is used as a medication to treat heart failure and atrial arrhythmias.

Cardiac glycosides work by inhibiting the sodium-potassium pump in heart muscle cells, leading to an increase in intracellular calcium levels. This increases the force of heart contractions, which can be beneficial in treating heart failure. However, if the dose is too high, cardiac glycosides can also cause dangerous arrhythmias and even death.

It's important for healthcare professionals to carefully monitor patients taking cardiac glycosides, as the therapeutic and toxic doses are very close together. Additionally, certain medications and medical conditions can interact with cardiac glycosides and increase the risk of toxicity.

Biotin is a water-soluble vitamin, also known as Vitamin B7 or Vitamin H. It is a cofactor for several enzymes involved in metabolism, particularly in the synthesis and breakdown of fatty acids, amino acids, and carbohydrates. Biotin plays a crucial role in maintaining healthy skin, hair, nails, nerves, and liver function. It is found in various foods such as nuts, seeds, whole grains, milk, and vegetables. Biotin deficiency is rare but can occur in people with malnutrition, alcoholism, pregnancy, or certain genetic disorders.

A DNA probe is a single-stranded DNA molecule that contains a specific sequence of nucleotides, and is labeled with a detectable marker such as a radioisotope or a fluorescent dye. It is used in molecular biology to identify and locate a complementary sequence within a sample of DNA. The probe hybridizes (forms a stable double-stranded structure) with its complementary sequence through base pairing, allowing for the detection and analysis of the target DNA. This technique is widely used in various applications such as genetic testing, diagnosis of infectious diseases, and forensic science.

Genital neoplasms in males refer to abnormal growths or tumors that develop in the male reproductive organs. These can be benign (non-cancerous) or malignant (cancerous).

Malignant genital neoplasms are often referred to as genital cancers. The most common types of male genital cancers include:

1. Penile Cancer: This occurs when cancer cells form in the tissues of the penis.
2. Testicular Cancer: This forms in the testicles (testes), which are located inside the scrotum.
3. Prostate Cancer: This is a common cancer in men, forming in the prostate gland, which is part of the male reproductive system that helps make semen.
4. Scrotal Cancer: This is a rare form of cancer that forms in the skin or tissue of the scrotum.
5. Penile Intraepithelial Neoplasia (PeIN): This is not cancer, but it is considered a pre-cancerous condition of the penis.

Early detection and treatment of genital neoplasms can significantly improve the prognosis. Regular self-examinations and medical check-ups are recommended, especially for individuals with risk factors such as smoking, HIV infection, or a family history of these cancers.

Nucleic acid hybridization is a process in molecular biology where two single-stranded nucleic acids (DNA, RNA) with complementary sequences pair together to form a double-stranded molecule through hydrogen bonding. The strands can be from the same type of nucleic acid or different types (i.e., DNA-RNA or DNA-cDNA). This process is commonly used in various laboratory techniques, such as Southern blotting, Northern blotting, polymerase chain reaction (PCR), and microarray analysis, to detect, isolate, and analyze specific nucleic acid sequences. The hybridization temperature and conditions are critical to ensure the specificity of the interaction between the two strands.

An oligonucleotide probe is a short, single-stranded DNA or RNA molecule that contains a specific sequence of nucleotides designed to hybridize with a complementary sequence in a target nucleic acid (DNA or RNA). These probes are typically 15-50 nucleotides long and are used in various molecular biology techniques, such as polymerase chain reaction (PCR), DNA sequencing, microarray analysis, and blotting methods.

Oligonucleotide probes can be labeled with various reporter molecules, like fluorescent dyes or radioactive isotopes, to enable the detection of hybridized targets. The high specificity of oligonucleotide probes allows for the precise identification and quantification of target nucleic acids in complex biological samples, making them valuable tools in diagnostic, research, and forensic applications.

In situ hybridization (ISH) is a molecular biology technique used to detect and localize specific nucleic acid sequences, such as DNA or RNA, within cells or tissues. This technique involves the use of a labeled probe that is complementary to the target nucleic acid sequence. The probe can be labeled with various types of markers, including radioisotopes, fluorescent dyes, or enzymes.

During the ISH procedure, the labeled probe is hybridized to the target nucleic acid sequence in situ, meaning that the hybridization occurs within the intact cells or tissues. After washing away unbound probe, the location of the labeled probe can be visualized using various methods depending on the type of label used.

In situ hybridization has a wide range of applications in both research and diagnostic settings, including the detection of gene expression patterns, identification of viral infections, and diagnosis of genetic disorders.

RNA probes are specialized biomolecules used in molecular biology to detect and localize specific RNA sequences within cells or tissues. They are typically single-stranded RNA molecules that have been synthesized with a modified nucleotide, such as digoxigenin or biotin, which can be detected using antibodies or streptavidin conjugates.

RNA probes are used in techniques such as in situ hybridization (ISH) and Northern blotting to identify the spatial distribution of RNA transcripts within cells or tissues, or to quantify the amount of specific RNA present in a sample. The probe is designed to be complementary to the target RNA sequence, allowing it to bind specifically to its target through base-pairing interactions.

RNA probes can be labeled with various reporter molecules, such as radioactive isotopes or fluorescent dyes, which enable their detection and visualization using techniques such as autoradiography or microscopy. The use of RNA probes has proven to be a valuable tool in the study of gene expression, regulation, and localization in various biological systems.

... may be conjugated to sugars to study glycosylation events, even in biological systems. Immunostaining "Digoxigenin ... Kd of the digoxigenin-antibody interaction has been estimated at ~12 nM (compare to Kd~0.1pM for the biotin-streptavidin ... Anti-digoxigenin antibodies with high affinities and specificity are used in a variety of biological immuno-assays (e.g. ELISA ... Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis ...
Panoskaltsis-Mortari, A.; Bucy, R. P. (February 1995). "In situ hybridization with digoxigenin-labeled RNA probes: facts and ... digoxigenin) is localized and quantified in the tissue using either autoradiography, fluorescence microscopy, or ...
digoxigenin ANS porphyrin BODIPY Cyanine Hybridization probe "Cancerweb Molecular Probe Definition". Sirbu, D.; Zeng, L.; ...
Another labelling protein, digoxigenin, is attached to the reference DNA sample. The labelled DNA samples are co-hybridized to ...
The hairpin is attached to the glass surface via a digoxigenin-antidigoxigenin bond. The magnetic bead is attached to the ... labeled with biotin and digoxigenin at the two ends. 2) For RNA-seq, the mRNA can be trapped on a poly-T-coated bead, where ...
... such as anti-digoxigenin) that will bind to an antigen (digoxigenin) at one end of the polymer. After an incubation of about ... One end has a biotin tail and the other has a digoxigenin tail. After incubation, unbound polymer has to be washed out from the ...
Specific Detection of Cherry Mottle Leaf Virus Using Digoxigenin-Labeled cDNA Probes and RT-PCR. Plant Disease, 83(3), 235-239 ...
ISBN 3-13-368515-5. Lanatoside C (isolanid, Cedilanid - four glycoside analog), Digoxigenin (aglycone analog) Goldberger ZD, ...
... and an aglycon named digoxigenin. Lypka A, Lazowski J (September 1979). "[Comparative study of blood serum concentrations of ...
If digoxigenin was used as a probe label, an anti-digoxigenin fluorescein primary antibody followed by a HRP-conjugated anti- ... Probe labelling can be carried out by using either random priming or nick translation to incorporate biotin or digoxigenin. For ... whereas CISH probes are labelled with biotin or digoxigenin and can be detected using a bright-field microscope after other ... labelling-fluorochromes are attached to the probes-or indirect labelling-the probes are labelled with biotin or digoxigenin ...
Digoxigenin can be used as a molecular probe to detect mRNA in situ and label DNA, RNA, and oligonucleotides. It can easily be ... Digoxigenin (DIG) is a steroid found in the flowers and leaves of Digitalis species, and is extracted from D. lanata. ... digoxigenin, diginatigenin and gitaloxigenin in the leaves act as a protection against herbivores. Yield and concentration of ...
Digoxigenin can be used as a molecular probe to detect mRNA in situ and label DNA, RNA, and oligonucleotides. It can easily be ... Digoxigenin (DIG) is a steroid found in the flowers and leaves of Digitalis species, and is extracted from D. lanata. ...
The latter is a common alternative to digoxigenin, and the two are used together for labelling two genes in one sample. ...
Some examples are the use of, digoxigenin- or fluorophore-conjugated oligo- nucleotide probes, for the detection of localized ... mRNAs in dendrites, spines, axons, and growth cones of cultured neurons; or digoxigenin-labeled RNA probes and fluorescence ...
Researchers have also recently created species-specific oligonucleotide primers and digoxigenin-labeled probes in hopes of ...
Members include: acetyldigitoxins acetyldigoxins cymarine digitoxin digitoxigenin digoxigenin digoxin medigoxin neoconvalloside ...
Albeit generic, the ligation probe is similar to a detection probe in that it is labelled with, for example, digoxigenin for ...
An enzyme-linked immunosorbent assay (ELISA) with digoxigenin-labeled antibodies can also be used to detect the toxin, and ... and F Clostridium botulinum neurotoxins in foods by using an amplified enzyme-linked immunosorbent assay with digoxigenin- ...
... such as digoxigenin and biotin chemistries, have been developed. Perceived disadvantages of Northern blotting are that large ...
... digoxigenin, a non-radioactive, antibody-based marker, biotin or fluorescein. DNA sequences or RNA transcripts that have ...
The molecular formula C23H34O5 (molar mass: 390.51 g/mol, exact mass: 390.2406 u) may refer to: Digoxigenin (DIG) Mevastatin, ...
England Digoxigenin or dig, a plant-produced steroid used for biochemical "tagging" Dig (restaurant), an American fast-casual ...
... digoxigenin MeSH D04.808.155.160.349.500 - medigoxin MeSH D04.808.155.160.861 - strophanthins MeSH D04.808.155.160.861.250 - ...
These common glycosides include digitoxigenin, gitoxigenin, digoxigenin, gitaloxigenin, and diginatigenin. The seeds of D. ...
Digoxigenin may be conjugated to sugars to study glycosylation events, even in biological systems. Immunostaining "Digoxigenin ... Kd of the digoxigenin-antibody interaction has been estimated at ~12 nM (compare to Kd~0.1pM for the biotin-streptavidin ... Anti-digoxigenin antibodies with high affinities and specificity are used in a variety of biological immuno-assays (e.g. ELISA ... Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis ...
View our 8 Digoxigenin Primary Antibodies for your research. ... Digoxigenin: Products. Digoxygenin (DIG) is a steroid that is ...
Digoxigenin-UTP, alkali-stable. Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of RNA probes ... Digoxigenin-UTP can replace UTP in reactions catalyzed by T3, T7 or SP6 RNA polymerases. The digoxigenin-labeled RNA ... Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of DNA probes. ≥93% (HPLC), ISH (in situ hybridization), ... The hybridized digoxigenin-labeled DNA probes can be detected by their interaction with antibodies coupled to fluorescent dyes ...
Home / AHR / Total RNA (10 g) was size-fractionated, blotted, and hybridized with digoxigenin-labelled IL-11 cDNA as described ... Total RNA (10 g) was size-fractionated, blotted, and hybridized with digoxigenin-labelled IL-11 cDNA as described in Strategies ... Total RNA (10 g) was size-fractionated, blotted, and hybridized with digoxigenin-labelled IL-11 cDNA as described in Strategies ... Total RNA (10 g) was size-fractionated, blotted, and hybridized with digoxigenin-labelled IL-11 cDNA as described in Strategies ...
Digoxigenin PubMed MeSh Term ©2023 Regents of the University of Colorado , Terms of Use , Powered by VIVO Data updated last 12/ ...
Anti-Digoxigenin Antibody. Catalogue Numbre: CDIG-65P, Supplied by Immunology Consultatnt Laboratory& Distributed by Gentaur in ...
Hit enter to search or ESC to close ...
Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 488. Catalog #: DI-7488-.5 ... Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 594. Catalog #: DI-7594-.5 ...
Chicken Anti-Keyhole Limpet Hemocyamin Manufactured by Gentaur. Gentaur is the biggest antibody manufacturer worldwide.
Modified NTPs: Biotin-, Fluorescein-, Digoxigenin-, Aminoallyl- Labeling: [α-32P] labeled ribonucleotide (800-6,000 Ci/mmol), ...
... digoxigenin, FITC, green). (f) N02Y5149: Th. intermedium (digoxigenin, FITC shown in red) and 45S rDNA (biotin, Alex594 shown ... intermedium DNA (biotin, Alexa594, red) and 120 bp repeat pSc119.2 (digoxigenin, FITC, green). (b) N02Y5018: genomic Th. ... intermedium DNA (biotin, Alexa594, red) and 5S rDNA (digoxigenin, FITC, green). (c) N02Y5003: genomic rye DNA (biotin, Alexa594 ... For labelling, biotin-16-dUTP and digoxigenin-11-dUTP (Roche Diagnostics, Basel, Switzerland) were incorporated in separate ...
Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for ... Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for ... Home / Products / Antibodies / Antibodies to Tags & Labels / Goat Anti-Digoxigenin/Digoxin (DIG) Antibody, DyLight™ 488. ...
Modified NTPs: Biotin-, Fluorescein-, Digoxigenin-, Aminoallyl- Labeling: [α-32P] labeled ribonucleotide (800-6,000 Ci/mmol), ...
Fluorescein-12-dUTP ≥85% (HPLC), suitable for ELISA, suitable for hybridization, solution; Synonyms: Fluorescein-12-dUTP,dUTP; find Roche-11373242910 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
Antisense labelled with digoxigenin RNA Visualized with: Alkaline phosphatase Assay notes: Cryosections of fresh frozen ...
... digoxigenin bisdigitoxoside; HPLC, high-performance liquid chromatography; AUC, area under the curve; MDR1, multidrug ...
Sheep IgG anti-digoxigenin Fab fragments, conjugated with horseradish peroxidase, 1207733 Roche; dilution: 1:500 for ... anti-digoxigenin-POD, sheep Fab fragments) was in 2% BSA in PBST at 4 °C for 48 h, followed by three washes with PBST. FISH ...
Digoxigenin-labeled antisense riboprobes were synthesized using standard protocols (Stern, 1998). The following probes were ...
Digoxigenin NHS-ester. CAS Number. 129273-26-3. Molecular Formula. C₃₅H₅₀N₂O₁₀. Appearance. White to Light Brown Solid. ...
In the following step, an antibody to digoxigenin conjugated to peroxidase (anti-DIG-POD) is added, then bound to digoxigenin. ... Components: hGH (human growth hormone); Anti-hGH-digoxigenin (anti-hGH-DIG) antibody, lyophilizate; Anti-Digoxigenin-POD ... Next, a digoxigenin-labeled antibody to hGH (anti-hGH-DIG) is added, then bound to hGH. ...
... digoxigenin-dUTP, and dATP or PBS in controls. Sections were then incubated with peroxidase-conjugated anti-digoxigenin ...
Incorporation of digoxigenin-11-dUTP during amplification allowed direct detection and quantitation of mRNA levels by ...
The HER-2/neu gene was localized using the Oncor (Gaithersburg, MD) digoxigenin-labeled unique sequence probe. Amplified PCs ...
Mix-n-Stain™ Digoxigenin. Antibody Labeling Kits. Digoxygenin (DIG). Mix-n-Stain™ DNP. Antibody Labeling Kits. DNP. ...
M. Tada and N. Ueno). For two-color in situ hybridization, RNA probes were labeled with digoxigenin for gsc and with ... Whole-mount in situ hybridization on zebrafish embryos using a mixture of digoxigenin- and fluorescein-labelled probes. Trends ...
A Digoxigenin-labeled probe derived from EHV 1 was used for hybridization. This probe hybridized specifically to sequences of ...
Digoxigenin (DIG)-labeled RNA sense and antisense probes were made from the linearized plasmids according to the manufacturers ...
... digoxigenin labeled UTP (Roche, Nutley, NJ), and a template amplified from cDNA by PCR. The template was a 624-bp region in the ... and anti-sense probes were digoxigenin-labeled RNA probes. The RNA probes were synthesized by in vitro transcription (Riboprobe ... 500 alkaline phosphatase-conjugated anti-digoxigenin antibody at room temperature. The slides were washed and incubated further ...
  • Kd of the digoxigenin-antibody interaction has been estimated at ~12 nM (compare to Kd~0.1pM for the biotin-streptavidin interaction). (wikipedia.org)
  • After incubation with an anti-digoxigenin antibody Xanthone (Genicide) conjugated with alkaline phosphatase (Boehringer Mannheim), membranes had been immersed in the chemiluminescent substrate, Xanthone (Genicide) CSPD (Tropix, Bedford, MA), and subjected to Fuji brand-new RX x-ray film (Fuji Image Film, Kanagawa, Japan). (mingsheng88.org)
  • The probe was labeled with digoxigenin, and hybridized fragments were detected with anti-digoxigenin antibody. (upenn.edu)
  • Anti-digoxigenin antibodies with high affinities and specificity are used in a variety of biological immuno-assays (e.g. (wikipedia.org)
  • The hybridized digoxigenin-labeled DNA probes can be detected by their interaction with antibodies coupled to fluorescent dyes or color-producing enzymes. (enzolifesciences.com)
  • Lanatoside C). Digoxigenin is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications similarly to other popular haptens such as 2,4-Dinitrophenol, biotin, and fluorescein. (wikipedia.org)
  • The HER-2/neu gene was localized using the Oncor (Gaithersburg, MD) digoxigenin-labeled unique sequence probe. (nih.gov)
  • A Digoxigenin-labeled probe derived from EHV 1 was used for hybridization. (uni-muenchen.de)
  • The specificity of the product was confirmed by hybridization to a digoxigenin-labeled probe according to the method described in Boehringer Mannhein's Genius System User's Guide for Filter Hybridization. (cdc.gov)
  • NGRL has been granted the license by "Roche Diagnostics GmbH" with regard to synthesis and sale and offering for sale of Digoxigenin labeling oligonucleotide(s) since 8th Dec. 1995. (ngrl.co.jp)
  • Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves), where it is attached to sugars, to form the glycosides (e.g. (wikipedia.org)
  • Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana" (pdf). (wikipedia.org)
  • Digoxigenin (DIG) is a small plant-derived molecule not found in animals. (vectorlabs.com)
  • The most commonly used labels for DNA probes include radioisotopes like Phosphorus 32, fluorophores like fluorescein, or small molecule binding partners such as biotin and digoxigenin. (jove.com)
  • Intracellular localization of parvovirus B19 nucleic acid at the ultrastructural level by in situ hybridization with digoxigenin-labelled probes. (ox.ac.uk)
  • Conditions suitable for immunogold detection of digoxigenin-labelled DNA probes hybridized to parvovirus B19-infected erythroid cells embedded in Lowicryl K4M and LR White acrylic resins were established at the electron microscope level. (ox.ac.uk)
  • We describe a method for direct QD labeling of modified oligonucleotide probes through streptavidin-biotin and antibody-mediated interactions (anti-FITC and anti-digoxigenin). (hindawi.com)
  • We target the gene of interest with dsDNA polynucleotide probes labeled multiple digoxigenins to which horse-raddish-peroxidase labeled antibodies bind. (mpi-bremen.de)
  • One of the probes is immobilized in microtiter wells, via the digoxigenin/anti-digoxigenin interaction, and the other probe is biotinylated. (uncg.edu)
  • Detection reagents are free for digoxigenin labeled probes. (pantomics.com)
  • In this article we describe a way for colorimetric recognition of miRNA in the kidney through hybridization with digoxigenin tagged microRNA probes. (immune-source.com)
  • Though we have found the 5' digoxigenin conjugated probes to provide us with sufficient detection sensitivity for our applications in kidney. (immune-source.com)
  • Anti-digoxigenin antibodies with high affinities and specificity are used in a variety of biological immuno-assays (e.g. (wikipedia.org)
  • Digoxigenin is thus an all-purpose immuno-tag, and in particular a standard immunohistochemical marker for in situ hybridization. (wikipedia.org)
  • L'étude menée en Iraq a utilisé la méthode d'hybridation in situ pour déterminer la fréquence du papillomavirus humain et pour son génotypage dans les échantillons de tissus prélevés auprès de 129 patientes ayant reçu un diagnostic de cancer du sein malin, de 24 patientes porteuses d'une tumeur du sein bénigne et de 20 femmes témoins en bonne santé. (who.int)
  • Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana" (pdf). (wikipedia.org)
  • Incorporation of digoxigenin-11-dUTP during amplification allowed direct detection and quantitation of mRNA levels by chemiluminescence. (jci.org)
  • Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. (wikipedia.org)
  • Knockdown of NEIL1 in LN428 glioblastoma cells resulted in enhanced recruitment of XPC, a more rapid removal of digoxigenin-tagged psoralen adducts, and decreased cellular sensitivity to trioxsalen, implying that NEIL1 and BER may interfere the processing of ICLs. (nih.gov)