Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
A group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in MOLECULAR GENETICS to detect the presence of a complementary sequence by NUCLEIC ACID HYBRIDIZATION.
A subspecialty of pathology concerned with the molecular basis (e.g., mutations) of various diseases.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
MOLECULAR BIOLOGY techniques used in the diagnosis of disease.
DNA probes specific for the human leukocyte antigen genes, which represent the major histocompatibility determinants in humans. The four known loci are designated as A, B, C, and D. Specific antigens are identified by a locus notation and number, e.g., HLA-A11. The inheritance of certain HLA alleles is associated with increased risk for certain diseases (e.g., insulin-dependent diabetes mellitus).
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
3 beta,12 beta,14-Trihydroxy-5 beta-card-20(22)-enolide. A cardenolide which is the aglycon of digoxin. Can be obtained by hydrolysis of digoxin or from Digitalis orientalis L. and Digitalis lanata Ehrh.
The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
Laboratory and other services provided to patients at the bedside. These include diagnostic and laboratory testing using automated information entry.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.
Techniques used in studying bacteria.
Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Methods, procedures, and tests performed to diagnose disease, disordered function, or disability.
DNA probes specific for the identification of human papilloma virus.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
The determination of the nature of a disease or condition, or the distinguishing of one disease or condition from another. Assessment may be made through physical examination, laboratory tests, or the likes. Computerized programs may be used to enhance the decision-making process.
The functional hereditary units of BACTERIA.
Any method used for determining the location of and relative distances between genes on a chromosome.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Techniques used to carry out clinical investigative procedures in the diagnosis and therapy of disease.
Deoxyribonucleic acid that makes up the genetic material of viruses.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
A tick-borne septicemic disease of domestic and wild ruminants caused by EHRLICHIA RUMINANTIUM.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.
Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
Therapeutic approach tailoring therapy for genetically defined subgroups of patients.
A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.
Incorrect diagnoses after clinical examination or technical diagnostic procedures.
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
A species of gram-negative bacteria in the family ANAPLASMATACEAE, that causes HEARTWATER DISEASE in ruminants.
The study of the structure, growth, function, genetics, and reproduction of viruses, and VIRUS DISEASES.
Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The preparation and analysis of samples on miniaturized devices.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
The male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans and in some other male-heterogametic species in which the homologue of the X chromosome has been retained.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
The specialty of ANALYTIC CHEMISTRY applied to assays of physiologically important substances found in blood, urine, tissues, and other biological fluids for the purpose of aiding the physician in making a diagnosis or following therapy.
Any technique by which an unknown color is evaluated in terms of standard colors. The technique may be visual, photoelectric, or indirect by means of spectrophotometry. It is used in chemistry and physics. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
Measurement of the intensity and quality of fluorescence.
Mapping of the KARYOTYPE of a cell.
A 60-kDa extracellular protein of Streptomyces avidinii with four high-affinity biotin binding sites. Unlike AVIDIN, streptavidin has a near neutral isoelectric point and is free of carbohydrate side chains.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Detection of a MUTATION; GENOTYPE; KARYOTYPE; or specific ALLELES associated with genetic traits, heritable diseases, or predisposition to a disease, or that may lead to the disease in descendants. It includes prenatal genetic testing.
A toxin produced by certain pathogenic strains of ESCHERICHIA COLI such as ESCHERICHIA COLI O157. It is closely related to SHIGA TOXIN produced by SHIGELLA DYSENTERIAE.
An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.
Compounds that contain three methine groups. They are frequently used as cationic dyes used for differential staining of biological materials.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
The design or construction of objects greatly reduced in scale.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Negative test results in subjects who possess the attribute for which the test is conducted. The labeling of diseased persons as healthy when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
Elements of limited time intervals, contributing to particular results or situations.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
Actual loss of portion of a chromosome.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Nanometer sized fragments of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY TECHNIQUES.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Any visual display of structural or functional patterns of organs or tissues for diagnostic evaluation. It includes measuring physiologic and metabolic responses to physical and chemical stimuli, as well as ultramicroscopy.
Proteins found in any species of bacterium.
A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed)
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.
Established cell cultures that have the potential to propagate indefinitely.
Abnormal number or structure of the SEX CHROMOSOMES. Some sex chromosome aberrations are associated with SEX CHROMOSOME DISORDERS and SEX CHROMOSOME DISORDERS OF SEX DEVELOPMENT.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
A species of gram-negative, aerobic bacteria primarily found in purulent venereal discharges. It is the causative agent of GONORRHEA.
The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition.
Nonexpendable items used in examination.
A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.
Ribonucleic acid that makes up the genetic material of viruses.
Acute infectious disease characterized by primary invasion of the urogenital tract. The etiologic agent, NEISSERIA GONORRHOEAE, was isolated by Neisser in 1879.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.
Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION.
A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.
Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.
A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).
The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.
Methods of creating machines and devices.
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
Diagnostic procedures, such as laboratory tests and x-rays, routinely performed on all individuals or specified categories of individuals in a specified situation, e.g., patients being admitted to the hospital. These include routine tests administered to neonates.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Antibodies that are chemically bound to a substrate material which renders their location fixed.
A genus of bacteria found in the reproductive organs, intestinal tract, and oral cavity of animals and man. Some species are pathogenic.
The study of parasites and PARASITIC DISEASES.
Techniques used in microbiology.
A complex that includes several strains of M. avium. M. intracellulare is not easily distinguished from M. avium and therefore is included in the complex. These organisms are most frequently found in pulmonary secretions from persons with a tuberculous-like mycobacteriosis. Strains of this complex have also been associated with childhood lymphadenitis and AIDS; M. avium alone causes tuberculosis in a variety of birds and other animals, including pigs.
DNA present in neoplastic tissue.
Diagnostic procedures involving immunoglobulin reactions.
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Determination of the nature of a pathological condition or disease in the postimplantation EMBRYO; FETUS; or pregnant female before birth.
Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC
The use of light interaction (scattering, absorption, and fluorescence) with biological tissue to obtain morphologically based information. It includes measuring inherent tissue optical properties such as scattering, absorption, and autofluorescence; or optical properties of exogenous targeted fluorescent molecular probes such as those used in optical MOLECULAR IMAGING, or nontargeted optical CONTRAST AGENTS.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
Uracil nucleotides which contain deoxyribose as the sugar moiety.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
The infiltrating of tissue specimens with paraffin, as a supporting substance, to prepare for sectioning with a microtome.
The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.
The relationships of groups of organisms as reflected by their genetic makeup.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues.
Determination of the quantity of a material present in a mixture by measurement of its effect on the electrical conductivity of the mixture. (Webster, 3d ed)
Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.
Facilities equipped to carry out investigative procedures.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A species of gram-negative bacteria in the genus PORPHYROMONAS, family Porphyromonadaceae. It is a key pathogen in endodontic infections.
So-called atypical species of the genus MYCOBACTERIUM that do not cause tuberculosis. They are also called tuberculoid bacilli, i.e.: M. buruli, M. chelonae, M. duvalii, M. flavescens, M. fortuitum, M. gilvum, M. gordonae, M. intracellulare (see MYCOBACTERIUM AVIUM COMPLEX;), M. kansasii, M. marinum, M. obuense, M. scrofulaceum, M. szulgai, M. terrae, M. ulcerans, M. xenopi.
Biochemical identification of mutational changes in a nucleotide sequence.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The clear, viscous fluid secreted by the SALIVARY GLANDS and mucous glands of the mouth. It contains MUCINS, water, organic salts, and ptylin.
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
The branch of medicine concerned with the application of NANOTECHNOLOGY to the prevention and treatment of disease. It involves the monitoring, repair, construction, and control of human biological systems at the molecular level, using engineered nanodevices and NANOSTRUCTURES. (From Freitas Jr., Nanomedicine, vol 1, 1999).
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
The rate dynamics in chemical or physical systems.
A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
A foul-smelling accumulation of SEBUM and desquaminated epidermal cells, especially the cheesy substance found under the foreskin of the penis and at the base of the labia minor near the clitoris.
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
One of the Type II site-specific deoxyribonucleases (EC It recognizes and cleaves the sequence G/AATTC at the slash. EcoRI is from E coliRY13. Several isoschizomers have been identified. EC 3.1.21.-.
Infections with bacteria of the genus CHLAMYDIA.
Infections with bacteria of the species ESCHERICHIA COLI.
Examination of the mouth and teeth toward the identification and diagnosis of intraoral disease or manifestation of non-oral conditions.
Agents which affect CELL DIVISION and the MITOTIC SPINDLE APPARATUS resulting in the loss or gain of whole CHROMOSOMES, thereby inducing an ANEUPLOIDY.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
Deoxyribonucleic acid that makes up the genetic material of protozoa.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Products or parts of products used to detect, manipulate, or analyze light, such as LENSES, refractors, mirrors, filters, prisms, and OPTICAL FIBERS.
Nucleotide sequences, generated by iterative rounds of SELEX APTAMER TECHNIQUE, that bind to a target molecule specifically and with high affinity.
The systematic study of the complete complement of proteins (PROTEOME) of organisms.
Controlled operation of an apparatus, process, or system by mechanical or electronic devices that take the place of human organs of observation, effort, and decision. (From Webster's Collegiate Dictionary, 1993)
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
Chromosomal, biochemical, intracellular, and other methods used in the study of genetics.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
Controlled operations of analytic or diagnostic processes, or systems by mechanical or electronic devices.
Substances elaborated by bacteria that have antigenic activity.
Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.
The specialty related to the performance of techniques in clinical pathology such as those in hematology, microbiology, and other general clinical laboratory applications.
The temperature at which a substance changes from one state or conformation of matter to another.
Oligonucleotides and Taq DNA polymerases for use in Molecular Diagnostic applications. Manufacturing processes take place in ... Real-time qPCR Probes, assay services, and proteomics. The proteomics operations are primarily concerned with custom peptides ... The in vitro diagnostics business unit provides technical and project support for contract manufacturing of good manufacturing ... 2008: Eurogentec received ISO 13485 Certification for the production and sales of In Vitro Diagnostics (IVD) oligonucleotides ...
FARMS has been designed for preprocessing and summarizing high-density oligonucleotide DNA microarrays at probe level to ... For targeted next-generation-sequencing panels in clinical diagnostics, in particular for cancer, Hochreiter's group developed ... A DNA segment is identical by state (IBS) in two or more individuals if they have identical nucleotide sequences in this ... cn.MOPS estimates the local DNA copy number, is suited for both whole genome sequencing and exom sequencing, and can be applied ...
... leading to the development of over 20 DNA diagnostic probes for evaluating aminoglycoside resistance in clinical isolates. ... Aminoglycoside resistance/diagnostics. As a leader in the field of aminoglycoside resistance Shaw was involved in the cloning ... Novel DNA Gyrase inhibitors. At Trius Pharmaceuticals, Shaw lead the microbiology efforts in designing a screening regimen for ... Morrow, Brian J.; Shaw, Karen Joy (2002), "DNA Microarray Expression Analysis in Antibacterial Drug Discovery", Pathogen ...
The HyX platform had five core technologies: Labeled DNA probes DNA arrays of samples and probes Software-driven modules like ... Hyseq's genomic platform was used to develop gene based therapeutics and diagnostics. At the rate of more than 1,000,000 human ... HyGnostics and HyChip Industrial robots to screen DNA probes Bioinformatics software for information management Applications ... Nuvelo was initially founded as Hyseq in August 1992 and based on DNA array technology. ...
... the target molecules are labeled with fluorescent tags and this biotin-labeled target DNA is hybridized with the probe DNA ... His group members are working on projects including cell lysis, PCR card, DNA hybridization, anthrax diagnostics and ... the probe molecules (biotin, DNA, or human plasma IgG) are covalently attached to the polycarbon surface via amide coupling ( ... is used to amplify the target genetic marker with the DNA-binding fluorescence dye or a sequence-specific probe is applied for ...
... in the DNA. Some examples of molecular diagnostic tests include DNA sequencing and Southern blotting.. College (4 yrs) → ... Fluorescence in situ hybridization (FISH) involves fluorescent labeling of probes that bind to specific DNA sequences, used for ... DNA sequencing is used to directly analyze the genomic DNA sequence of a particular gene. In general, only the parts of the ... Diagnostic evaluation[edit]. Each patient will undergo a diagnostic evaluation tailored to their own particular presenting ...
Nucleic acid probes for many viruses. DNA fingerprinting techniques for characterization and documentation of germplasm. ... Development of Diagnostics and to offer diagnostic services to SAUs, Farmers and Private seed and Tissue culture industries. ... Diagnostics : Diagnosis of diseases of various horticultural crops and advice on their control and management. Supply of Video ... Integrated disease management protocols and diagnostic kits for viruses. Post Harvest Technology : IIHR has contributed to the ...
Each probe consists of two oligonucleotides which recognize adjacent target sites on the DNA. One probe oligonucleotide ... Identification of deletions or duplications can indicate pathogenic mutations, thus MLPA is an important diagnostic tool used ... The signal strengths of the probes are compared with those obtained from a reference DNA sample known to have two copies of the ... If the probes were not split in this way, the primer sequences at either end would cause the probes to be amplified regardless ...
Dual Hybridization (LightCycler®) probes Scorpions® Probes LUX (Light Upon Extension) Probes DNA binding dye assays (e.g., SYBR ... nucleic acid detection Real-time PCR quantification Allelic discrimination and identification Multiplex PCR assays Diagnostic ... Molecular beacons, or molecular beacon probes, are oligonucleotide hybridization probes that can report the presence of ... Fluorogenic signaling oligonucleotide probes were reported for use to detect and isolate cells expressing one or more desired ...
... negatively charged DNA and molecular probes can be concentrated on energized electrodes for interaction Using photolithography ... Some of its major applications include genomics, proteomics, molecular diagnostics, point-of-care diagnostics, tissue ... The reaction involves thermal cycling of the DNA sequence and DNA polymerase through three different temperatures. Heating up ... Michigan probes have been used in large-scale recordings and network analysis of neuronal assemblies , and the Utah electrode ...
It consists of fluorescently labeled DNA probes to the pericentromeric regions of chromosomes 3 (red), 7 (green), and 17 (aqua ... Abbott Moledular: UroVysion Bladder Cancer Kit For In Vitro Diagnostic Use v t e v t e. ...
... with these fragments being used as probes on the DNA microarray. Now probes of various origins such as cDNA, genomic PCR ... making it far more adaptable to diagnostic uses. The DNA on the slide is a reference sample, and is thus obtained from a ... repetitive DNA regions like centromeres and telomeres need to be blocked with unlabeled repetitive DNA (e.g. Cot1 DNA) and/or ... DNA from the sample to be tested is labeled with a red fluorophore (Cyanine 5) and a reference DNA sample is labeled with green ...
... complex simultaneous probe hybridization and direct mutation detection to help solve the dual problem of multiple sample ... developed a new approach that provided a breakthrough in dealing with bubbles using the multiplex allele-specific diagnostic ... In bioinformatics, a DNA read error occurs when a sequence assembler changes one DNA base for a different base. The reads from ... Mechanisms of DNA Recombination and Genome Rearrangements: Intersection Between Homologous Recombination, DNA Replication and ...
Sensors may pick up on abnormalities with DNA as well as the presence of antibodies. An ultrasonic probe can plug into a ... There are reports that medical tricorders may emerge from "diagnostic medical apps" via Tablet Computers and smartphones. Some ... This British biotech firm is developing a device described as a "handheld DNA lab" to analyze malaria; the firm is raising ... It developed a virus detection device called Q-POC which breaks open cells to analyze their DNA. Ibis Biosciences. This firm ...
The use of a strand-displacing DNA polymerase in LAMP also precludes the use of hydrolysis probes, e.g. TaqMan probes, which ... In a diagnostic application, this must be balanced against the need to choose an appropriate target (e.g., a conserved site in ... gene using loop-mediated isothermal amplification combined with DNA functionalized gold nanoparticles as probes". Aquaculture. ... likely due to use of a different DNA polymerase (typically Bst - Bacillus stearothermophilus - DNA polymerase rather than Taq ...
... has been used to study DNA-protein interactions (using a terbium chelate complex) to measure distances in DNA ... Traditional virus diagnostic procedures are being replaced by sensitive immunoassays with lanthanides. The time resolved ... Lanthanide probes ligands must meet several chemical requirements for the probes to work properly. These qualities are: water ... Several systems have been proposed which combine MRI capability with lanthanides probes in dual assays. The luminescent probe ...
For the diagnostics of beta-thalassemia mutations, a simple chip was designed that contained six probes corresponding to ... By performing this reaction at a temperature above the melting temperature of the duplex between the DNA and immobilized probe ... A set of potential hybridization probes are created for each DNA sequence that form perfect duplexes with that sequence. The ... probes) onto these gel pads. Test sample is then added to this micromatrix containing immobilized probes in gel pads and ...
In the diagnostic laboratory virus infections can be confirmed by a multitude of methods. Diagnostic virology has changed ... Detection of viral RNA and DNA genomes can be performed using polymerase chain reaction. This technique makes many copies of ... the virus genome using virus-specific probes. Variations of PCR such as nested reverse transcriptase PCR and real time PCR can ... Serology Molecular diagnostics Leland, D. S.; Ginocchio, C. C. (2007). "Role of Cell Culture for Virus Detection in the Age of ...
DAPI is a dye that binds to DNA and fluoresces blue. For this reason, it is particularly useful at labeling nuclei. Antibody ... However, the increased rate of binucleation is usually not high enough to make it a conclusive diagnostic tool. The fate of ... probes can be used to label tubulin fluorescently. The immunofluorescence may then be observed with microscopy. Binucleated ...
... on which pieces of DNA (probes) are affixed in a microscopic array. Similar to a DNA microarray, a protein array is a miniature ... Yetisen A. K. (2013). "Paper-based microfluidic point-of-care diagnostic devices". Lab on a Chip. 13: 2210. doi:10.1039/ ... DNA chips (microarrays)[edit]. Early biochips were based on the idea of a DNA microarray, e.g., the GeneChip DNAarray from ... Microfluidics emerged in the beginning of the 1980s and is used in the development of inkjet printheads, DNA chips, lab-on-a- ...
In HFI, the diagnosis of homozygotes is difficult, requiring a genomic DNA screening with allele specific probes or an enzyme ... avoid testing via fructose challenge as the results are non-conclusive for individuals with HFI and even if the diagnostic ...
In situ hybridization - Specific DNA and RNA molecules can be identified on sections using this technique. When the probe is ... In others, they are in essence clinician group practices that employ pathologists to provide diagnostic services for the group ... all in addition to the practice of diagnostic anatomical pathology. Pathologists in academic settings often sub-specialize in a ... will control a partnership that employs junior pathologists and contracts independently with hospitals to provide diagnostic ...
Probe size is important because longer probes hybridize less specifically than shorter probes, so that short strands of DNA or ... it may lead to more portable diagnostic techniques.[18][19] ... Probes - RNA and DNA[edit]. ViewRNA detection of miR-133(green ... Repetitive DNA sequences must be blocked by adding short fragments of DNA to the sample. The probe is then applied to the ... Preparing DNA probes for one species and performing FISH with this probe allows one to visualize the distribution of this ...
Early in the 1970s, they exploited Avidin as a probe and developed new methods and reagents to biotinylate antibodies and other ... Today, the system is applied in research and diagnostics as well as medical devices and pharmaceuticals. Examples include ... DNA, hormone, etc.) without disturbing the interaction with its target molecule; avidin is then used to "sandwich" between the ... Consequently, the avidin-biotin system can frequently replace radioactive probes. Together with Ed Bayer, Wilchek established ...
SNP stands for Single nucleotide polymorphism probes. These probes allow for increased precision and greater diagnostic yield. ... prenatal and pediatric diagnostics, offering DNA-based testing for the detection of genetic abnormalities beyond what can be ... and opened a diagnostics lab in Irvine, CA, which was originally led by former US Labs employees with the intent of entering ... The median spatial resolution between probes is 1 Kb within gene rich regions and 5 Kb outside of gene-rich regions. Lash, Alex ...
C. immitis and C. posadasii cannot be distinguished on cytology or by symptoms, but only by DNA PCR. An indirect demonstration ... Research demonstrates that patients from endemic areas who are aware of the disease are most likely to request diagnostic ... It can also be detected in culture by morphological identification or by using molecular probes that hybridize with C.immitis ... The fungal infection can be demonstrated by microscopic detection of diagnostic cells in body fluids, exudates, sputum and ...
Conversely, LNA-incorporated probes demonstrated increased hybridization efficiency in both DNA and RNA. The improved ... Due to its high affinity for mismatch discrimination, LNA has been studied for its applications in diagnostic tools. ... LNA nucleotides can be mixed with DNA or RNA residues in the oligonucleotide, in effect hybridizing with DNA or RNA according ... "Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes". Applied and Environmental ...
... a capability key to various diagnostic and therapeutic protocols. Breslauer has mapped the energetic consequences of DNA damage ... The database and its extensions also have been used to design probes and drugs with predictable hybridization and binding ... At Rutgers since 1974, he combines these fields by investigating protein-DNA, and drug-DNA interactions, particularly as they ... PREDICTING DNA DUPLEX STABILITY FROM THE BASE SEQUENCE, KJ Breslauer et al., PNAS Vol 83, pp 3746-3750, June 1986[permanent ...
"Imec releases neuropixels neural probe to the global Neuroscience Community". www.imec-int.com. "Imec tool accelerates DNA ... "Imec awarded NASA grant to advance space health diagnostics". www.newelectronics.co.uk. "The programmable brain-on-a-chip". www ... In 2017, IMEC designed and fabricated the world's first miniature neural probe for simultaneous recording of multiple brain ... neural probes, lab-on-chip, organ-on-chip), wireless communication (e.g. 5G and wireless IoT communication), image sensors, and ...
In DNA-based diagnostic tests, complexes are used to determine whether electrons can flow across the DNA. If an electron could ... By using specially-designed chiral metal complexes as probes of DNA she has been able to study how DNA is damaged and repaired ... Her experiments reveal a strategy for how DNA repair proteins locate DNA lesions and demonstrate a biological role for DNA- ... This view of DNA is deeper and more dynamic than previous views of DNA as a static library. Understanding the mediation of ...
Molecular and Cellular Probes. 31: 22-27. doi:10.1016/j.mcp.2016.08.003. PMID 27523487. Archived (PDF) from the original on 20 ... Diagnostic method. Based on symptoms, tick exposure, blood tests[3]. Prevention. Prevention of tick bites (clothing the limbs, ... The examination of preserved museum specimens has found Borrelia DNA in an infected Ixodes ricinus tick from Germany that dates ... Except for one study in Europe,[229] much of the data implicating lizards is based on DNA detection of the spirochete and has ...
Fluorescein is currently used most as a fluorescent probe. Equipment that can automatically measure and calculate the capacity ... and protection of DNA, proteins and lipids from oxidative damage pursuant to Article 13(1) of Regulation (EC) No 1924/20061". ... is commercially available (Biotek, Roche Diagnostics). The fluorescent intensity decreases as the oxidative degeneration ... "Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe ...
These results, found by probing with immunoassays, have been challenged by studies that interrogate DNA sequence by bisulfite ... This DNA-histone structure is called a nucleosome; the more tightly the DNA is bound by the nucleosome, and the more tightly a ... In mammals, methylation occurs on DNA and histone proteins. DNA methylation occurs on the cytosine of CpG dinucleotides in the ... DNA methylation. In Alzheimer's disease cases, global DNA hypomethylation and gene-specific hypermethylation has been observed ...
New Probe Detects Trace Pollutants in Groundwater". Oak Ridge National Laboratory Review. 26 (2).. ... SERS can be used to target specific DNA and RNA sequences using a combination of gold and silver nanoparticles and Raman-active ... fundamental aspects and diagnostic applications". Analytical and Bioanalytical Chemistry. 407 (27): 8265-8277. doi:10.1007/ ... The gold nanoparticles facilitate the formation of a silver coating on the dye-labeled regions of DNA or RNA, allowing SERS to ...
quantitative PCR does not require this, as the detection system uses fluorescence and probes to detect the DNA molecules as ... Diagnostic tests[edit]. Main article: Diagnostic microbiology. Identification of an infectious agent for a minor illness can be ... This would prove invaluable for diagnostic techniques.[7] Commonly treated infectious diseases[edit]. Bacterial[edit]. * ... DNA sequencing, a method developed by Walter Gilbert and Frederick Sanger in 1977,[5] caused a rapid change the development of ...
Using RT-PCR, diagnostic results can be available in one to two days.[56] Serological diagnosis requires a larger amount of ... a nonstructural protein that degrades RBP1 and turns off the host cell's ability to transcribe DNA.[51] NS2 interferes with the ... "Probing the attenuation and protective efficacy of a candidate chikungunya virus vaccine in mice with compromised interferon ( ...
"Standard Mutation Nomenclature in Molecular Diagnostics" The Journal of Molecular Diagnostics 9 (1), 2007 ... Kaasaegsete andmete kohaselt erineb kahe eri inimese DNA kuni 0,1% ehk vähem kui 3 miljonit DNA aluspaari. Erinevates ... "Genotyping of triallelic SNPs using TaqMan® PCR" Molecular and Cellular Probes 21 (3): 171-176. ... DNA molekul 1 erineb DNA molekulist 2 ühe aluspaari juures (C/T polümorfism). ...
Green signal from anti-tubulin antibody conjugated with Alexa Fluor 488) and nuclei (blue signal from DNA stained with DAPI) in ... CLSM has the advantage of not requiring a probe to be suspended nanometers from the surface, as in an AFM or STM, for example, ... Medical diagnostics. MeSH. D018613. OPS-301 code. 3-301. [edit on Wikidata] ...
"Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA". Electrophoresis. National Diagnostics. Retrieved 13 October 2016. ... Studies comparing different denaturation methods such as heating, beads mill of different bead sizes, probe sonification, and ... DNA repair or protein binding are set to occur.[17] The area of partially separated DNA is known as the denaturation bubble, ... Melting curves of DNA". doi:10.2172/5693881.. *^ López-Alonso JP, Bruix M, Font J, Ribó M, Vilanova M, Jiménez MA, Santoro J, ...
... also called DNA probes, to reconstruct a target DNA sequence. Non-specific hybrids are removed by washing and the target DNA is ... "The Journal of Molecular Diagnostics. 10 (6): 484-92. doi:10.2353/jmoldx.2008.080027. PMC 2570630 . PMID 18832462.. ... large DNA fragments into shorter DNA fragments. The fragmented DNA may then be cloned into a DNA vector and amplified in a ... DNA nanoball sequencing[edit]. Main article: DNA nanoball sequencing. DNA nanoball sequencing is a type of high throughput ...
... cells grown in culture and stained with antibody to tubulin (green), antibody to Ki-67 (red) and the blue DNA binding dye ... Dual-color FISH with a c-MYC probe mapping to 8q24 revealed colocalization with HPV18 at all integration sites, indicating that ... heptamethine dyes IR-808 and other analogs which are currently being explored for their unique uses in medical diagnostics, the ... Human papillomaviruses (HPVs) are frequently integrated into the cellular DNA in cervical cancers. We mapped by FISH five HPV18 ...
The filter and colonies are prepared for hybridization and then labeled with a probe. The target DNA- insert of interest- can ... Such genome-wide assessments may lead to further diagnostic and drug therapies while also helping future teams focus on ... The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a ... Use the enzyme DNA ligase to seal the DNA fragments into the vector. This creates a large pool of recombinant molecules. These ...
Polzer et al, EMBO 2014, Molecular profiling of single Circulating Tumor cells with diagnostic intention Polzer Et all EMBO ... DNA, chromosomes, proteins and viruses. DEP can be used to separate particles with different sign polarizabilities as they move ... thus when probed with a light beam the well the light intensity increases through the well. The opposite is true for negative ... DEP is being applied in fields such as medical diagnostics, drug discovery, cell therapeutics, and particle filtration. ...
... thus allowing the use of small exogenous molecules as biomolecular probes. A fluorophore can be attached to one of these probes ... Rieder, Ulrike; Luedtke, Nathan W. (25 August 2014). "Alkene-tetrazine ligation for imaging cellular DNA". Angew Chem Int Ed ... Integrated Diagnostics,[58] and the biotech company baseclick, a BASF spin-off created to sell products made using click ... Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called ...
... found in DNA upstream of each constant region gene (except in the δ-chain). The DNA strand is broken by the activity of a ... Goding J (1978). "Allotypes of IgM and IgD receptors in the mouse: a probe for lymphocyte differentiation". Contemp Top ... Detection of particular antibodies is a very common form of medical diagnostics, and applications such as serology depend on ... RAG proteins play an important role with V(D)J recombination in cutting DNA at a particular region.[39] Without the presence of ...
This diagnostic fold can also be found in the MalF and MalG TM subunits of MalFGK2 and the Met transporter MetI. In the MetI ... The third subgroup of ABC proteins do not function as transporters, but are rather involved in translation and DNA repair ... the modulation of the accumulation of a probe substrate (e.g. fluorescent dyes like rhodamine 123, or calcein) can be ... The name ATP-binding cassette is derived from the diagnostic arrangement of the folds or motifs of this class of proteins upon ...
One notable use is diagnostic X-ray imaging in medicine (a process known as radiography). X-rays are useful as probes in high- ... UV rays in the middle range can irreparably damage the complex DNA molecules in the cells producing thymine dimers making it a ... gamma rays are used for diagnostic imaging in nuclear medicine, an example being PET scans. The wavelength of gamma rays can be ...
Two probes must be used for each SNP position to detect both alleles; if only one probe were used, experimental failure would ... The basic principles of SNP array are the same as the DNA microarray. These are the convergence of DNA hybridization, ... Sheils, O; Finn, S; O'Leary, J (2003). "Nucleic acid microarrays: An overview". Current Diagnostic Pathology. 9 (3): 155-8. doi ... In molecular biology, SNP array is a type of DNA microarray which is used to detect polymorphisms within a population. A single ...
In May 2015, Pfizer and a Bar-Ilan University laboratory announced a partnership based on the development of medical DNA ... "Congress joins probe into Wyeth's Rapamune marketing". FiercePharma. Retrieved July 11, 2011 ... "Pfizer to collaborate on Bar-Ilan DNA robots". Globes. Retrieved June 6, 2015 ...
"Proteomics of mouse BRCA1-deficient mammary tumors identifies DNA repair proteins with potential diagnostic and prognostic ... Multiplex Ligation-dependent Probe Amplification (MLPA),[55] and Quantitative Multiplex PCR of Short Fluorescent Fragments ( ... If DNA repair is deficient, DNA damage tends to accumulate. Such excess DNA damage may increase mutational errors during DNA ... BRCA1 directly binds to DNA, with higher affinity for branched DNA structures. This ability to bind to DNA contributes to its ...
"gen-probe.com. Archived from the original on 12 December 2012.. *↑ 107.0 107.1 Dunne EF, Nielson CM, Stone KM, Markowitz LE, ... HPV DNA హైబ్రిడైజింగ్ HPV DNA ను కిట్ నుండి పూర్తిస్థాయి RNA సన్నివేశాలతో క్లినికల్ నమూనాల నుండి గుర్తించడం కోసం ఇది ఒక ణొన్ ... "Chapter 5: Technological Accretion in Diagnostics: HPV Testing and Cytology in Cervical Cancer Screening". మెడికల్ ఇన్నోవేషన్ ... లో వైరస్ యొక్క ఒకటి లేదా అంతకంటే ఎక్కువ జాతుల DNA ను కనుగొన్నారు.[76] ఏది ఏమయినప్పటికీ, రక్తములో HPV DNA యొక్క ఉనికిని ...
sensitive non-radioactive in situ hybridization probes to detect nucleic acids in plants, able to detect 1 µg of plasmid DNA.[6 ... 2002). DIG Application Manual for Nonradioactive in situ Hybridization (3rd ed.). Penzberg: Roche Diagnostics.. ...
Diagnostic method. Stool culture, testing for the bacteria's DNA or toxins[1]. ... "Scientists probe whether C. difficile is linked to eating meat". CBC News. 4 October 2006. Archived from the original on 24 ... The appearance of "pseudomembranes" on the mucosa of the colon or rectum is highly suggestive, but not diagnostic of the ... Stool leukocyte measurements and stool lactoferrin levels also have been proposed as diagnostic tests, but may have limited ...
DNA sequencing theory. References[edit]. *^ a b c Staden, R (1979). "A strategy of DNA sequencing employing computer programs" ... The end of one of these clones can then be sequenced to yield a new probe and the process repeated in a method called ... 2009). "Next Generation Sequencing: From Basic Research to Diagnostics". Clinical Chemistry. 55 (4): 41-47. doi:10.1373/ ... In genetics, shotgun sequencing is a method used for sequencing long DNA strands. It is named by analogy with the rapidly ...
Detection of DNA using these methods can only be seen after the hybridization of probes with its complementary DNA takes place ... The primers used must be specific to the targeted sequences in the DNA of a virus, and PCR can be used for diagnostic analyses ... including a DNA template that contains the DNA target region to amplify; a DNA polymerase; an enzyme that polymerizes new DNA ... Digital PCR (dPCR): used to measure the quantity of a target DNA sequence in a DNA sample. The DNA sample is highly diluted so ...
... and director of the Centre for DNA Fingerprinting and Diagnostics, which conducted the DNA testing for the Aarushi case). ... "Deputation of CBI officer probing Noida case ends". The Times of India. 2 July 2008.. ... The CBI then sent the sample for further testing to the Centre for DNA Fingerprinting and Diagnostics (CDFD) in Hyderabad. The ... The kukri was then sent to the CFSL DNA expert BK Mahapatra, who said he could not extract any DNA from the weapon.[120] ...
In the medical diagnostics laboratories of the 21st century, the ability to detect nucleic ... the potential of DNA probes-based diagnostics is huge. DNA probes also render themselves as a powerful tool for chromosomal ... Roche Diagnostics, Siemens Healthcare Diagnostics, among others.. The research report titled "DNA Probes-Based Diagnostics: A ... Burgeoning Molecular Diagnostics Market Propels the Global DNA Probes-Based Diagnostics Market, According to New Report by ...
About DNA Probe-based Diagnostic. The DNA probe is a basic component in most molecular diagnostic techniques. The DNA probe is ... Global DNA probe-based diagnostics market by infectious disease testing. *Global DNA probe-based diagnostics market by cancer ... Exhibit 16: DNA probe-based diagnostics market in EMEA 2016-2021 ($ billions). Exhibit 17: DNA probe-based diagnostics market ... Exhibit 19: DNA probe-based diagnostics market in APAC 2016-2021 ($ billions). Exhibit 20: DNA probe-based diagnostics market ...
This econometric study covers the world outlook for DNA probes-based - Market research report and industry analysis - 7785572 ... The 2013-2018 World Outlook for DNA Probes-Based Diagnostics The 2013-2018 World Outlook for DNA Probes-Based Diagnostics ... The 2013-2018 World Outlook for DNA Probes-Based Diagnostics. This econometric study covers the world outlook for DNA probes- ... Life Sciences»Biotechnology»DNA Sequencing The 2013-2018 World Outlook for DNA Probes-Based Diagnostics. Lowest Prices ...
Nano, Adela and Barton, Jacqueline (2016) Rhodium-conjugate fluorescent probes for diagnostic of mismatched DNA. In: 252nd ... probes capable of detecting and signaling DNA base mismatches directly would be a powerful tool to this end. Previous studies ... In healthy cells, DNA base pair mismatches, as well as some other base pair anomalies derived from DNA base damage, are cor. ... measurements in the presence of well matched and mismatched DNA support the notion that our systems are interesting probes for ...
Diagnostics,Market:,DNA,Probes,and,Biochips,--,New,Product,Development,Opportunities,and,Business,Expansion,Strategies,for, ... The molecular diagnostics market is unquestionably the most rapidly gr...,Reportlinker,Adds,Molecular, ... Blood Supply, Tissue Banking and Transplantation Industry: Molecular Diagnostics Market: DNA Probes and Biochips -- New Product ... Reportlinker Adds Molecular Diagnostics Market: DNA Probes and Biochips -- New Product Development Opportunities and Business ...
Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing Legionnaires disease in 167 ... Diagnostic efficacy of a DNA probe in pneumonia caused by Legionella species J Med Microbiol. 1993 Mar;38(3):183-6. doi: ... A commercial DNA probe kit (Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing ... The diagnostic performance of the DNA probe assay in this study was superior to that of the DFA test. The results indicate that ...
DNA Probe-based Diagnostic Market Growth, Key Futuristic, Future Opportunities and Forecast from 2017 to 2022 - published on ... 1 Industry Overview of DNA Probe-based Diagnostic. 1.1 DNA Probe-based Diagnostic Market Overview. 1.1.1 DNA Probe-based ... 1.3 DNA Probe-based Diagnostic Market by Type. 1.3.1 DNA Probes-Based Products. 1.3.2 Direct Detection of Target Sequence. 1.3. ... Gene Detection Using DNA Chips. Gene Detection by DNA Sequencing. Market segment by Application, DNA Probe-based Diagnostic can ...
The latest market intelligence study on DNA Probe-based Diagnostic market relies on statistics derived from the application of ... opportunities and competitive landscape of DNA Probe-based Diagnostic market for the forecast period, 2018 - 2025. Importantly ... DNA Probe-Based Diagnostic Market Set for Rapid Growth 2018-2025. DNA Probe-based Diagnostic Market report helps to increase ... Scope of DNA Probe-based Diagnostic Market Research Report-. In addition, the research on the DNA Probe-based Diagnostic market ...
World Market Segmentation by City The 2016 Report on DNA Probes-Based Diagnostics: World Market Segmentation by City This ... Life Sciences»Diagnostics»In Vitro Diagnostics»Molecular Diagnostics The 2016 Report on DNA Probes-Based Diagnostics: World ... The 2016 Report on DNA Probes-Based Diagnostics: World Market Segmentation by City. This report was created for global ... In what follows, I summarize the economic potential for the worlds major cities for "DNA probes-based diagnostics" for the ...
This report analyzes the worldwide markets for DNA Probes-Based Diagnostics in US$ Million by the following Application Areas: ... What are DNA-Probes?. DNA-based Diagnostics - A Prelude. DNA Tests: Finding Use in Numerous Applications. DNA Probes: Robust ... DNA Chips. Chromosome Painting Probes. Diagnostic Applications Based on DNA Probes: An Overview. Infectious Diseases. ... 3.DNA PROBES-BASED DIAGNOSTICS - A PRIMER. Diagnostic Gene Detection Products. Molecular Diagnostics: A Powerful Disease ...
DNA Probe-based Diagnostic market is segmented by region (country),... ... 130 Pages Report] Check for Discount on Global and Japan DNA Probe-based Diagnostic Market Insights, Forecast to 2026 report by ... revenue generated in DNA Probe-based Diagnostic business, the date to enter into the DNA Probe-based Diagnostic market, DNA ... U.A.E DNA Probe-based Diagnostic Sales Growth Rate (2015-2020) (K Units). Figure 106. U.A.E DNA Probe-based Diagnostic Revenue ...
Roche Diagnostics Deutschland GmbH, Roche NimbleGen, a company of Roche announced today the launch of comparative genomic ... Roche Diagnostics Deutschland GmbH * BoxId: 209139 - Roche NimbleGen Launches 2.1 Million-Probe Arrays for the Detection of ... Roche NimbleGen Launches 2.1 Million-Probe Arrays for the Detection of Chromosomal Aberrations and DNA Copy Number Variation. ... Recommend Roche NimbleGen Launches 2.1 Million-Probe Arrays for the Detection of Chromosomal Aberrations and DNA Copy Number ...
Adenoviruses of subgroups B, C and E were detected equally well by probes prepared either from purified adenovirus type 5 or ... The use of stable non-radioactive probes makes in situ hybridization a feasible assay for use in clinical laboratories with ... Adenovirus DNA was detected in cells from nasopharyngeal secretions of children with acute respiratory infections by in situ ... hybridization with biotinylated probes. The technique was easy to perform, giving rapid results which were well correlated with ...
A flow cell which can be used in the synthesis of DNA probes on an active surface of the substrate includes a base having a ... Methods for producing uniquely specific nucleic acid probes. WO2011128096A1. Apr 14, 2011. Oct 20, 2011. Roche Diagnostics Gmbh ... A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:. (a) a base having ... 1. A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:. (a) a base ...
... the Market for DNA/RNA Probe Diagnostic Systems Identification of Challenges Facing the Market for DNA/RNA Probe Diagnostic ... and Market SegmentationIntroduction to DNA/RNA Probe Diagnostic Systems MarketIntroduction to DNA/RNA Probe Diagnostic Systems ... United States DNA/RNA Probe Diagnostic System Markets 22-Feb-1999 / Research Code: 5847-01-00-00-00 / SKU: HC00280-NA-MR_03295 ... United States DNA/RNA Probe Diagnostic System Markets, Executive SummaryObjectives and PurposeObjectives and PurposeReport ...
Foresight Diagnostics Commercializing PhasED-seq Liquid Biopsy Tech for Improved MRD Detection Premium ...
Diagnostics DNA probes Drug delivery Enabling technology Equipment Genomics Natural product Oligonucleotide Orphan drug ... Recombinant DNA Regenerative medicine Research services Research supplies Screening Small molecules Stem cells ...
Global DNA Probes-Based Diagnostic Market Research Report Forecast 2017 to 2021 includes market share, market research report, ... Chapter 1 DNA Probes-Based Diagnostic Market Overview.  1.1 Product Overview and Scope of DNA Probes-Based Diagnostic. & ... nbsp3.5.1 DNA Probes-Based Diagnostic Market Concentration Rate.  3.5.2 DNA Probes-Based Diagnostic Market Share of Top 3 ... nbsp6.3 Global DNA Probes-Based Diagnostic Price by Type (2012-2017).  6.4 Global DNA Probes-Based Diagnostic Production ...
Specific DNA probes were developed for An. punctulatus, An. koliensis, and three sibling species, An. farauti No. 4, An. ... 6, by differentially screening total genomic DNA libraries of individual species and sibling species with homologous DNA ... Probes ranged from 273 to 630 bp. Identification of species can be made from squash or dot blots using only a segment of the ... Abstract Genomic DNA probes were made for five members of the Anopheles punctulatus complex of mosquitoes found in Papua New ...
University of Idaho VIVO is a discovery tool that enables anyone to find experts, research, and associated activities at the University of Idaho.
About the VENTANA HER2 Dual ISH DNA Probe Cocktail assay. Roche is the only provider of a fully automated brightfield Dual ISH ... Roche (SIX: RO, ROG; OTCQX: RHHBY) today announced the launch of the new VENTANA HER2 Dual ISH DNA Probe Cocktail assay for the ... The VENTANA HER2 Dual ISH DNA Probe Cocktail assay3 aids in identifying breast and gastric cancer patients eligible for the ... The VENTANA HER2 Dual ISH DNA Probe Cocktail assay is designed to be completed within the same day, providing clinicians the ...
The diagnostic performance of the DNA probe assay in this study was superior to that of the DFA test. The results indicate that ... Depending on the diagnostic criteria, the probe test had a sensitivity of 31-67%, a specificity of 99% and positive predictive ... The probe assay result was positive in six patients; five of them had other laboratory evidence of disease (positive cultures ... Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing Legionnaires' disease ...
... quickly and at low cost using a single set of random DNA probes. Rices universal microbial diagnostic, ... ... Universal microbial diagnostics using random DNA probes, Science Advances (2016). DOI: 10.1126/sciadv.1600025 ... If the DNA from a patients sample binds with the complementary DNA in a disease probe, the diagnosis is positive for that ... quickly and at low cost using a single set of random DNA probes. Rices "universal microbial diagnostic," or UMD, uses pieces ...
Diagnostic deoxyribonucleic acid probes for infectious diseases. Clin. Microbiol. Rev. 1:82-101.PubMedPubMedCentralCrossRef ... Molecular cloning of a species-specific DNA probe for Campylobacter jejuni. Mol. Cell. Probes 1:245-259.PubMedCrossRefGoogle ... DNA probes assays for detection of Campylobacter and Salmonella. In Nucleic Acid and Monoclonal Antibody Probes, ed. B. ... Development of a Salmonella-specific biotinylated DNA probe for rapid routine identification of Salmonella. Mol. Cell. Probes 2 ...
7.3 Diagnostics. 7.4 Therapeutics. 7.4.1 RNAi. 7.4.2 Nucleic Acid Aptamers. 7.4.3 DNA/Antisense Oligonucleotides. 7.4.4 ... Oligonucleotide Synthesis Market by Product (Primer, Probe, Linker, Adaptor, Custom, Reagent, Equipment), Application (PCR, DNA ... Oligonucleotide Synthesis Market by Product (Primer, Probe, Linker, Adaptor, Custom, Reagent, Equipment), Application (PCR, DNA ... Table 10 Market for Probes, By Region, 2015-2022 (USD Million). Table 11 North America: Market for Probes, By Country, 2015- ...
DNA buoyant density shifts during 15N-DNA stable isotope probing. Microbiol. Res. 162:328-334. ... Roche Diagnostics. 2009. Using multiplex identifier (MID) adaptors for the GS FLX titanium chemistry-extended MID set. ... DNA-based SIP (15N-SIP) is often complicated by a small degree of DNA separation between labeled and unlabeled DNA molecules ( ... DNA precipitation, and DNA quantitation were carried out as described previously (5, 6). In brief, equal amounts of DNA from ...
Specific DNA probes in diagnostic microbiology. US4366241 *. 7 Aug 1980. 28 Dec 1982. Syva Company. Concentrating zone method ... Relia Diagnostic Systems, Llc. Lateral flow system and assay. US8221995. 27 Jun 2008. 17 Jul 2012. Seok-Won Lee. Methods and ... Diagnostic markers of acute coronary syndrome and methods of use thereof. US20030119064 *. 20 Aug 2002. 26 Jun 2003. Valkirs ... Relia Diagnostic Systems, Llc. Bidirectional lateral flow test strip and method. US7358055. 17 Aug 2005. 15 Apr 2008. Biosite, ...
DNA Probes-based diagnostics deals with the study to examine the chemical structure of DNA by undoing the presence of abnormal ... Also, U.S. and Europe together holds the majority of the global DNA Probes-based diagnostics market as about 80% to 90% of DNA ... The DNA Probes-based technology is extensively used in diagnostic and also in other fields such as instrumentation development ... Worldwide DNA Probes - Based Diagnostics Market - Global Industry Analysis, Size, Share, Growth, Trends, and Forecast, 2012 - ...
2017 Nanomedical Diagnostics, a life science company pioneering the use of label-free graphene biosensors in the healthcare ... New Patent Granted to Nanomedical Diagnostics for Use of Graphene Biosensors with DNA Probes to Identify DNA Sequences for ... New Patent Granted to Nanomedical Diagnostics for Use of Graphene Biosensors with DNA Probes to Identify DNA Sequences for ... The patent covers use of graphene biosensors with DNA probes to detect nucleic acids, and it builds on a previous comprehensive ...
  • The results indicate that the examination of respiratory tract secretions by the Gen-probe kit is a suitable screening test for the diagnosis of Legionnaires' disease. (nih.gov)
  • If the DNA from a patient's sample binds with the complementary DNA in a disease probe, the diagnosis is positive for that disease. (phys.org)
  • These probes have shown rapid and cost-effective microbial diagnosis, good sensitivity and specificity for some periodontal pathogens in cases of severe periodontitis. (thejcdp.com)
  • The three types of bacteria can be detected rapidly with high sensitivity by using the DNA probe by general practitioners, and thus can help in the diagnosis process and the treatment. (thejcdp.com)
  • The products under development include FISH Probes for the diagnosis of renal cancer, HPV-associated cancer test and Microarrays for the diagnosis of leukamia, ovarian cancer, cervical cancer, bladder cancer, prostate cancer amongst others. (sbwire.com)
  • This comprehensive seven-country report will assist diagnostics industry executives, as well as companies planning to diversify into the dynamic and rapidly expanding microbiology market, in evaluating emerging opportunities and developing effective business strategies. (aarkstore.com)
  • The spectroscopic measurements in the presence of well matched and mismatched DNA support the notion that our systems are interesting probes for mismatch detection and signaling. (caltech.edu)
  • A commercial DNA probe kit (Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing Legionnaires' disease in 167 patients with pneumonia. (nih.gov)
  • OTCQX: RHHBY) today announced the launch of the new VENTANA HER2 Dual ISH DNA Probe Cocktail assay for the detection of the HER2 biomarker in breast and gastric cancer. (roche.com)
  • 1989. Detection of rotavirus by hybridization with a nonradioactive synthetic DNA probe and comparison with commercial enzyme immunoassays and silver stained Polyacrylamide gels. (springer.com)
  • Detection of enterotoxigenic Escherichia coli by dot blot hybridization with biotinylated DNA probes. (springer.com)
  • The new patent enables the company's graphene biosensor technology to bring the benefits of lower costs, easier process, and higher throughput to the world of liquid biopsy and other DNA detection applications. (evonexus.org)
  • Nanomedical Diagnostics ("Nanomed") is a life science company based in San Diego, CA. Nanomed has developed a breakthrough electrical assay based on proprietary Field Effect Biosensing (FEB) technology that delivers sensitive real-time direct detection of biomolecules. (evonexus.org)
  • An extensive review of DNA probe and biochip technologies, test formats, detection methodologies, trends in testing automation and amplification methods. (mynewsdesk.com)
  • Incorporating an immunomagnetic separation (IMS) step, followed by a secondary enrichment culturing step and DNA recovery with a QIAamp tissue kit (Qiagen), improved the detection threshold to ≥10 2 CFU/ml. (asm.org)
  • With an optimal DNA extraction method, LiPA allows rapid detection of M. tuberculosis complex DNA and RMP resistance directly from sputum specimens. (asm.org)
  • These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. (mdpi.com)
  • Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit , enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. (neb.com)
  • Yahfoufi ZA, Hadchiti W, Berberi A. Deoxyribonucleic Acid Probes Analyses for the Detection of Periodontal Pathogens. (thejcdp.com)
  • Deoxyribonucleic acid probes can detect bacteria at very low level about 10 3 which is below the detection level of culture methods. (thejcdp.com)
  • DNA probe detection of periodontal pathogens. (thejcdp.com)
  • Many diagnostic assays have been developed which are based on either immunochemical detection of proteins, e.g. (google.com)
  • or the detection of nucleic acids, either RNA or DNA, present in or produced by target cells, e.g. (google.com)
  • A chemical method for fast and sensitive detection of DNA synthesis in vivo. (abcam.com)
  • Labelling and detection of probes. (muni.cz)
  • The diagnostic performance of the DNA probe assay in this study was superior to that of the DFA test. (nih.gov)
  • The VENTANA HER2 Dual ISH DNA Probe Cocktail assay 3 aids in identifying breast and gastric cancer patients eligible for the targeted Roche drug Herceptin (trastuzumab), providing fast results with widely available laboratory instruments. (roche.com)
  • The VENTANA HER2 Dual ISH DNA Probe Cocktail assay is designed to be completed within the same day, providing clinicians the ability to get results back quicker than the most common methods of confirmatory testing for HER2. (roche.com)
  • The new VENTANA HER2 Dual ISH assay advances Roche's commitment to personalized healthcare by delivering critical information on treatment options for breast and gastric cancer patients faster," said Michael Heuer, CEO Roche Diagnostics. (roche.com)
  • For more information about the assay, please visit the Roche Tissue Diagnostics breast cancer IHC/ISH portfolio page or the Anatomic Pathology site. (roche.com)
  • With the new VENTANA HER2 Dual ISH DNA Probe Cocktail assay, Roche provides an improved brightfield assay that is fully automated on the BenchMark IHC/ISH instruments. (roche.com)
  • The patent covers use of graphene biosensors with DNA probes to detect nucleic acids, and it builds on a previous comprehensive patent for monitoring biomolecular interactions on which the company's flagship product, the Agile R100 personal assay system, is based. (evonexus.org)
  • Hybridization assay employing labeled probe and anti-hybrid. (freepatentsonline.com)
  • In this report, we describe the development and evaluation of the sensitivity and specificity of a PCR-based 5′ nuclease assay for presumptively detecting E. coli O157:H7 DNA. (asm.org)
  • The APTIMA Trichomonas vaginalis Assay (Gen-Probe, San Diego, Calif) uses nucleic acid hybridization technology to detect the presence of T vaginalis . (medscape.com)
  • The BD ProbeTec TV Qx Amplified DNA Assay is a DNA-amplification test developed for the BD Viper system, a proprietary automated platform capable of testing for T vaginalis , Chlamydia trachomatis , N gonorrhoeae , HSV-1, and HSV-2. (medscape.com)
  • The life science business unit specializes in genomics, and involves the development of oligonucleotides, DNA polymerases, Real-time qPCR Probes, assay services, and proteomics. (wikipedia.org)
  • Roche NimbleGen is a leading innovator, manufacturer, and supplier of a proprietary suite of DNA microarrays, consumables, instruments and services. (pressebox.com)
  • The improved performance is made possible by Roche NimbleGen's proprietary Maskless Array Synthesis (MAS) technology, which uses digital light processing and rapid, high-yield photochemistry to synthesize long oligo, high-density DNA microarrays with extreme flexibility. (pressebox.com)
  • Compressive sensing DNA microarrays. (nih.gov)
  • The company offers products such as CGH Microarrays, Next-Generation Sequencing and DNA-FISH Probes. (mynewsdesk.com)
  • Represented biotechnology company in patent litigation relating to methods for manufacturing DNA microarrays. (cov.com)
  • More recent genomic identification methods that use polymerase chain reaction, or PCR, and genomic sequencing can be faster but require expensive equipment and training as well as a specific DNA probe for each pathogen to be tested. (phys.org)
  • Sanger DNA Sequencing g. (aarkstore.com)
  • Different methods of sequencing of nucleic acids and analysis of DNA sequences. (muni.cz)
  • Recombinant human and murine IFN-γ were purchased from Biosource Europe and Roche Diagnostics, respectively. (rupress.org)
  • Total RNA was extracted using a MagnaPure LC RNA Isolation Kit (Roche Diagnostics). (rupress.org)
  • RT- and real-time PCR reactions were then performed using LightCycler-RNA Master Hybridization Probes (one-step procedure) on a Lightcycler ® apparatus (Roche Diagnostics). (rupress.org)
  • Roche NimbleGen, a company of Roche announced today the launch of comparative genomic hybridization (CGH) arrays and services for the high-resolution mapping of genomic copy-number differences based on the company's new 2.1 million-probe HD2 platform. (pressebox.com)
  • These new multiplex arrays allow the parallel hybridization of four samples to four separate 72,000 long oligo probe arrays on a single slide, providing a cost-effective, increased throughput option for CGH analysis. (pressebox.com)
  • In situ hybridization with biotinylated DNA probes: a rapid diagnostic test for adenovirus upper respiratory infections. (semanticscholar.org)
  • Adenovirus DNA was detected in cells from nasopharyngeal secretions of children with acute respiratory infections by in situ hybridization with biotinylated probes. (semanticscholar.org)
  • Hybridization-capture polymerase chain reaction (HC-PCR), a nucleic acid sequence capture technique, was evaluated on faecal samples pooled from 50 sheep and individual faecal samples as a rapid diagnostic test for Mycobacterium avium subsp. (nih.gov)
  • The lack of sensitivity of HC-PCR was neither due to location nor length of capture probe on IS 900 nor deterioration of the probe but was associated with inefficiencies in liquid phase hybridization and solid phase magnetic bead capture. (nih.gov)
  • more particularly, the invention provides compositions for detecting activated T-cells by nucleic acid hybridization probes and/or immunochemical assays. (google.com)
  • The molecular diagnostics market is unquestionably the most rapidly growing segment of the in vitro diagnostics industry. (bio-medicine.org)
  • Roche is the world leader in in-vitro diagnostics and drugs for cancer and transplantation, and is a market leader in virology. (pressebox.com)
  • The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally. (google.com)
  • 2. Outlook for the In Vitro Diagnostics Market a. (reportsnreports.com)
  • Manufacturers of in vitro diagnostics are increasingly calling on suppliers to assist them in meeting growing demands of industry regulators in both the US and Europe. (sigmaaldrich.com)
  • Importantly, the regulations also apply to in vitro diagnostics, including the chemical and biological components they contain, so that any raw materials destined for a diagnostic or to be used in the manufacture of a diagnostic kit must meet specific requirements. (sigmaaldrich.com)
  • 2007: In order to fully meet the Oligonucleotide in vitro diagnostics stringent manufacturing requirements, Eurogentec expanded its current GMP facility in Liège. (wikipedia.org)
  • 2008: Eurogentec received ISO 13485 Certification for the production and sales of In Vitro Diagnostics (IVD) oligonucleotides in Liège. (wikipedia.org)
  • Eurogentec has developed three interrelated business units, Life Science Research Products and Services, In Vitro Diagnostics Manufacturing Solutions, and GMP BioManufacturing. (wikipedia.org)
  • The in vitro diagnostics business unit provides technical and project support for contract manufacturing of good manufacturing practice (GMP) Oligonucleotides and Taq DNA polymerases for use in Molecular Diagnostic applications. (wikipedia.org)
  • Previous studies have shown that rhodium metalloinsertors bind mismatches in double-strained DNA with high specificity. (caltech.edu)
  • Antibodies are highly sought after for diagnostic applications owing to its specificity and affinity towards a target antigen. (intechopen.com)
  • Results using either method must be interpreted with considerable caution, because poor sensitivity and specificity of these diagnostic tests has been demonstrated in several studies. (medscape.com)
  • Rice's "universal microbial diagnostic," or UMD, uses pieces of randomly assembled DNA and mathematical techniques that were originally pioneered for signal processors inside digital phones and cameras. (phys.org)
  • With universal microbial diagnostic, we won't need new probes or to change any other parts of the sensing hardware," he said. (phys.org)
  • Recently, Deoxyribonucleic acid (DNA)-based techniques have been introduced to detect human microbial pathogens in periodontal diseases. (thejcdp.com)
  • Controls were included for all reactions to exclude amplification of contaminating genomic DNA. (rupress.org)
  • Amplification methods in molecular diagnostics of microorganisms. (muni.cz)
  • Amplification of target DNA sequence, amplification of probe, anplification of signal carried on a probe. (muni.cz)
  • The Global DNA Probes-Based Diagnostic Market Research Report Forecast 2017-2021 is a valuable source of insightful data for business strategists. (researchnreports.com)
  • Rice University researchers (clockwise from left) Richard Baraniuk, Amirali Aghazadeh and Rebekah Drezek have invented a technology that could potentially identify hundreds of bacterial pathogens simply, quickly and at low cost using a single set of random DNA probes. (phys.org)
  • Rice University scientists have invented a technology that could potentially identify hundreds of bacterial pathogens simply, quickly and at low cost using a single set of random DNA probes. (phys.org)
  • In a paper online this week in Science Advances, Rice's research team used lab tests to verify that UMD could identify 11 known strains of bacteria using the same five random DNA probes. (phys.org)
  • Specific oligonucleotide probes were developed to identify medically important fungi that display yeast-like morphology in vivo. (asm.org)
  • Specific oligonucleotide probes to identify these fungi, as well as a probe to detect all dimorphic, systemic pathogens, were developed. (asm.org)
  • κB#1wt probe was generated by end labeling the double stranded oligonucleotide 5′-AGAGTCCCGGGAAAGTCCTGCCGCGCC-3′ (corresponding to nt −68 to −42), and electrophoretic mobility shift assays (EMSAs) were performed as described previously ( 9 ). (rupress.org)
  • Eurogentec operates two manufacturing facilities in Belgium that provide custom biologics and oligonucleotide-based components for diagnostic and therapeutic/prophylactic applications. (wikipedia.org)
  • Two years after EGT NA also received ISO 13485 certification for the Oligonucleotide diagnostics manufacturing. (wikipedia.org)
  • Because the probes are not specific to a particular disease, the technology provides a genomic-based bacterial identification system that does not require a one-to-one ratio of DNA probes to pathogenic species. (phys.org)
  • The lack of rapid bacterial diagnostics can promote antibiotic resistance. (phys.org)
  • Several families of Proteobacteria and Actinobacteria that are directly involved in petroleum degradation incorporated the added nitrogen in contaminated soils, but it was the DNA of Sphingomonadaceae that was most enriched in 15 N. Bacterial growth in uncontaminated soils was not stimulated by nutrient amendment. (asm.org)
  • As a global leader in breast cancer diagnostics, Roche provides a comprehensive menu of both diagnostic and predictive assays, including the VENTANA HER2/neu (4B5) Rabbit Monoclonal Primary Antibody that is indicated as an aid in the assessment of breast cancer patients for whom Herceptin treatment is considered. (roche.com)
  • Monoclonal antibodies to pneumocystis proteins and sensitive DNA probes have been developed but have yet to reach the bedside. (bmj.com)
  • This comprehensive seven-country report is designed to help current suppliers and potential market entrants identify and evaluate emerging opportunities in the global transfusion diagnostics market during the next ten years, and assist industry executives in developing effective business, new product development and marketing strategies. (bio-medicine.org)
  • Assessment of current and emerging technologies, and their potential applications for the transfusion diagnostics market. (bio-medicine.org)
  • Infectious Diseases represents the key end-use market for DNA probes-based diagnostics worldwide. (prweb.com)
  • The major application area for DNA Probes-based diagnostics market includes Infectious Diseases, genetic predispositions, Molecular HLA (Human Leukocyte Antigen) typing, Forensic, Cancver testing, and others. (blogspot.com)
  • The infectious diseases segment exhibits the largest application segment for DNA Probes-based diagnostics market. (blogspot.com)
  • Reportlinker Adds Molecular Diagnostics Market: DNA Probes and Biochips -- New Product Development Opportunities and Business Expansion Strategies for Instrument and Reagent. (bio-medicine.org)
  • DNA Probe-based Diagnostic market is segmented by region (country), players, by Type, and by Application. (reportsnreports.com)
  • Based on product, the phytopathological disease diagnostics market is segmented into kits, lateral flow devices, DNA/RNA probes, accessories, other consumables, and software and cloud databases. (pharmiweb.com)
  • The U.S. holds a major share of the global DNA Probes-based diagnostics market as transformational technologies enabling the complex genetic tests to shift from research only laboratories to medical and clinical laboratories. (blogspot.com)
  • The main goal of LightDyNAmics is to achieve a complete understanding of the ultrafast dynamical processes at the molecular scale induced by UV light absorption in DNA, and to unveil the mechanisms leading to photodamage of the genetic code. (europa.eu)
  • This panel of DNA probes is designed to provide diagnostic genetic information for patients with myeloproliferative disorders. (legacyhealth.org)
  • It offers cancer diagnostics and treatments tailoring the specific genetic profile of the individual. (sbwire.com)
  • Genetic Variability in Nuclear DNA in Field Populations of Stagonospora nodorum . (apsnet.org)
  • The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. (muni.cz)
  • The new study includes several computer simulations, including one that shows how a random selection of five probes can identify 40 different strains of bacteria, and another that demonstrates how the system can accurately differentiate between 24 different species of Staphylococcus. (phys.org)
  • Various procedures and probes are available for the analysis of bacteria isolated from shell disease lesions (Chistoserdov et al. (gc.ca)
  • The innuSPEED Bacteria/Fungi DNA Kit has been specially devel-oped for isolating genomic DNA from gram+ bacteria, fungal spores and yeasts. (environmental-expert.com)
  • Deoxyribonucleic acid probes can detect bacteria at a very low level if we compared with the culture methods. (thejcdp.com)
  • The global market for DNA Probe-Based Diagnostics is forecast to reach US$30 billion by the year 2017, driven by the rapidly expanding molecular diagnostics market. (prweb.com)
  • New York, NY -- ( SBWIRE ) -- 04/16/2018 -- The latest market intelligence study on DNA Probe-based Diagnostic market relies on statistics derived from the application of both primary and secondary research to present insights pertaining to the operational model, opportunities and competitive landscape of DNA Probe-based Diagnostic market for the forecast period, 2018 - 2025. (sbwire.com)
  • The research also segments the DNA Probe-based Diagnostic market on the basis of the end-user, product type, application and demography for the forecast period 2018 - 2025. (sbwire.com)
  • For more clarity on the real potential of the DNA Probe-based Diagnostic market for the forecast period of 2018 - 2025 the study provides vital intelligence on the major opportunities, threats and challenges posed by the industry. (sbwire.com)
  • What is estimated growth rate and market size of the DNA Probe-based Diagnostic industry for the forecast period 2018 - 2025? (sbwire.com)
  • Which market trends from the yesteryears and the future are likely to keep the prospect of the DNA Probe-based Diagnostic market high for the forecast period 2018 - 2025? (sbwire.com)
  • This chapter explains the key trends that are expected to influence the growth of the phytopathological disease diagnostics market during the forecast period. (pharmiweb.com)
  • This section explains the global market value analysis and forecast for the phytopathological disease diagnostics during the forecast period. (pharmiweb.com)
  • The report provides annual sales estimates and projections for DNA Probe-Based Diagnostics market for the years 2009 through 2017 for the following geographic markets - US, Canada, Japan, Europe, Asia-Pacific and Latin America. (prweb.com)
  • Roche NimbleGen uniquely produces high-density arrays of long oligo probes that provide greater information content and higher data quality necessary for studying the full diversity of genomic and epigenomic variation. (pressebox.com)
  • Genomic DNA probes were made for five members of the Anopheles punctulatus complex of mosquitoes found in Papua New Guinea. (ajtmh.org)
  • farauti No. 6, by differentially screening total genomic DNA libraries of individual species and sibling species with homologous DNA against heterologous DNA labeled with 32 P. Probes ranged from 273 to 630 bp. (ajtmh.org)
  • The innuSPEED Tissue DNA Kit has been specially designed for using homogenizers in the process of isolating genomic DNA from an exceptionally wide variety of starting materials. (environmental-expert.com)
  • In conclusion, probes developed to yeast-like pathogens were found to be highly specific and should prove to be useful in differentiating these organisms in the clinical setting. (asm.org)
  • This study shows a high frequency of putative periodontal pathogens by using DNA probe technology, which is semi-quantitative in this study. (thejcdp.com)
  • Nanomedical Diagnostics , a life science company pioneering the use of label-free graphene biosensors in the healthcare industry, announces the grant of U.S. Patent No. 9,765,395 by the United States Patent and Trademark Office. (evonexus.org)
  • The extraordinary sensitivity of graphene biosensors also creates the opportunity to detect very low concentrations of circulating tumor DNA, possibly improving patient outcomes. (evonexus.org)
  • As the world's only mass-manufacturer of graphene biosensors, Nanomed's mission is to transform the healthcare industry with innovative new products that enable cutting-edge life science research, drug discovery applications, and diagnostic and health monitoring platforms. (evonexus.org)
  • In order to successfully capitalize on the opportunities presented by the molecular diagnostics market, many companies are already exploiting new DNA probe and biochip technologies as corporate strategic assets, managed in support of business and marketing strategies. (bio-medicine.org)
  • The probes are snippets of complementary DNA from a known disease. (phys.org)
  • Rather than identifying a target strain based on a 100 percent match with a specific probe, Rice's system tests how well the target DNA binds with several different random segments of complementary DNA. (phys.org)
  • This will be achieved by performing independent, yet interrelated and complementary research projects focussed on photoactivated dynamics of DNA, and by developing a variety of new spectroscopic and computational methods. (europa.eu)
  • The latter assays use nucleic acid probes, usually fluorescently labeled or radioactively labeled DNAs or RNAs, which can be preferentially hybridized to complementary target nucleic acids in appropriately prepared samples or tissues. (google.com)
  • Over 60 specific opportunities for molecular diagnostic instruments, test systems, IT and auxiliary products. (mynewsdesk.com)
  • The CDC now recommends molecular diagnostic tests, when available, to evaluate patients at risk for trichomoniasis. (medscape.com)
  • Antibodies have been utilized successfully in various diagnostic platforms ranging from standard immunoassays to lateral-flow assays, nanoparticles, microfluidics, DNA‐integrated assays and others. (intechopen.com)
  • this obviates the need for bronchoscopy but the diagnostic sensitivity is lower. (bmj.com)
  • Follow us on LinkedIn - In the medical diagnostics laboratories of the 21st century, the ability to detect nucleic acids and proteins reign supreme. (prweb.com)
  • Dr. Stole was a post-doctoral fellow at Johns Hopkins University (1992-1996) where he conducted research on enzymes involved in reactions with and repair of DNA and other nucleic acids. (cov.com)
  • If a laboratory today wants to test for 200 known pathogenic species, they need 200 different tests, each with its own specific DNA probe that was designed specifically to bind with DNA from a particular pathogen," said study co-author Richard Baraniuk, the lead scientist on the new study. (phys.org)
  • The report is based on combination of primary and secondary information sources, including interviews with blood bankers, laboratory directors, and executives of leading diagnostics companies in the U.S., five major European countries, and Japan. (bio-medicine.org)
  • Major issues pertaining to the Japanese molecular diagnostics laboratory practice, as well as key economic, regulatory, demographic, social and technological trends with significant market impact during the next five years. (mynewsdesk.com)
  • Azar MM, Hage CA. Laboratory diagnostics for histoplasmosis. (cdc.gov)
  • Liquid biopsy has shown tremendous promise for cancer diagnostics, but current methods have cost and process challenges to overcome," says Nanomedical Diagnostics CEO, Ross Bundy. (evonexus.org)
  • Required Textbook: Molecular Diagnostics: Fundamentals, Methods and Clinical Applications, 2nd edition. (austincc.edu)
  • Moreover, the true burden of fungal disease is most certainly underestimated because of the insensitivity of present diagnostic methods ( 8 , 18 , 25 ). (asm.org)
  • The invention relates to nucleic acid multiplexes, and more particularly to methods wherein quadruplexes are formed by specific binding between double-stranded nucleic acid probes and double-stranded nucleic acid target sequences. (google.com)
  • Dur- ing the first week, the biology, molecular biology and pathophysiology of myco- plasmas, as well as all the main diagnostic methods were covered, including both conventional and the newer technologies. (springer.com)
  • Dr. Stole's litigation and counseling experience has focused on matters involving pharmaceuticals, chemicals, chemical processes, and biotechnology such as genetically-engineered enzymes and DNA-based diagnostic methods. (cov.com)
  • A recent market study published by Future Market Insights (FMI) on the phytopathological disease diagnostics including global industry analysis of 2014-2018 and opportunity assessment for 2019-2029, delivers a comprehensive assessment of the most important market dynamics. (pharmiweb.com)
  • 1986. Probing of Campylobacter jejuni with DNA coding for Escherichia coli heat-labile enterotoxin. (springer.com)
  • To assess the utility of this test for detecting Mycobacterium tuberculosis complex DNA and rifampin (RMP) resistance, 420 sputum samples comprising specimens from untreated ( n = 160) and previously treated ( n = 260) patients from 11 countries in Asia, Africa, Europe, and Latin America were tested. (asm.org)
  • The LiPA test was positive for M. tuberculosis complex DNA in 389 (92.9%) specimens, including 92.0% (286 of 311) ZN-positive and 94.5% (103 of 109) ZN-negative specimens. (asm.org)
  • LiPA detected M. tuberculosis complex DNA in 92.4% (110 of 119) of the culture-positive and 100.0% (41 of 41) of the culture-negative specimens from untreated patients. (asm.org)
  • Growth in the marketplace is also expected to stem from affluent population and widening health insurance as well as medical reimbursements, which are making expensive cutting edge diagnostic tools such as DNA-based diagnostics increasingly affordable. (prweb.com)
  • In the US, the rapid growth of DNA-based diagnostics is leading to the development of new tests for the treatment of non-contagious diseases, such as diseases related to aging or chronic diseases. (prweb.com)
  • The research report titled "DNA Probes-Based Diagnostics: A Global Strategic Business Report" announced by Global Industry Analysts Inc., provides a comprehensive review of the DNA probe-based diagnostics markets, current market trends, key growth drivers, recent product introductions, recent industry activity, and profiles of major/niche global as well as regional market participants. (prweb.com)
  • In addition, the research on the DNA Probe-based Diagnostic market concentrates on extracting valuable data on swelling investment pockets, significant growth opportunities and major market vendors to help understand business owners what their competitors are doing best to stay ahead in the competition. (sbwire.com)
  • It provides the DNA Probes-Based Diagnostic industry overview with growth analysis and historical & futuristic cost, revenue, demand and supply data (as applicable). (researchnreports.com)
  • After conducting thorough research on the historical as well as current growth parameters of the phytopathological disease diagnostics market, growth prospects are obtained with maximum precision. (pharmiweb.com)
  • com/research/rgm4tc/20152019_global) has announced the addition of the "2015-2019 Global Pseudomonas Diagnostic Testing Market: Emerging Opportunities and Growth Strategies" report to their offering. (thefreedictionary.com)
  • Also, the boom in the molecular diagnostics market is beginning to echo downstream into the DNA-probe based diagnostics segment. (prweb.com)
  • It also includes market size and revenue distribution of market segment of the phytopathological disease diagnostics market. (pharmiweb.com)
  • Progress towards a rapid polymerase chain reaction diagnostic test for the identification of Mycobacterium avium subsp. (nih.gov)
  • A flow cell which can be used in the synthesis of DNA probes on an active surface of the substrate includes a base having a central window opening and a registration surface against which the substrate may be mounted. (google.com)
  • Alternative to BrdU (5-Bromo-2'-deoxyuridine) ( ab142567 ) for measuring de novo DNA synthesis during S-phase. (abcam.com)
  • Used for probing short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required. (abcam.com)
  • Represented biotechnology company in patent litigation involving synthesis and use of DNA libraries. (cov.com)
  • 1999: Eurogentec acquired Oswel Research Product Ltd. a biotechnology company based in Southampton, Great Britain and specialized in the synthesis of chemically modified and complex oligonucleotides and their analogues (DNA, RNA, PNA). (wikipedia.org)
  • DNA Probe-based Diagnostic Market 2017-2022, has been prepared based on an in-depth market analysis with inputs from industry experts. (openpr.com)
  • Specific DNA probes were developed for An. (ajtmh.org)
  • The innuPREP Virus DNA/RNA allows researchers to purify viral ssDNA or dsDNA and ssRNA simultaneously from serum, plasma or other cell-free bodily fluids, from tissue, paraffin or swab samples, or from cell cultures. (environmental-expert.com)
  • Molecular diagnostics, such as PCR on tissue specimens, are increasingly available to support microscopic findings, although the performance of these tests may vary. (cdc.gov)
  • TaqMan probes, hybridisation probes, molecular beacons. (muni.cz)
  • DNA was extracted from sputum samples by using a modification of the Boom's method, while the rpoB core region was amplified by nested PCR. (asm.org)
  • Integrating new technology planning with business and corporate strategies will be one of the most challenging tasks for diagnostics companies during the next ten years. (bio-medicine.org)
  • The DNA Probes-based technology is extensively used in diagnostic and also in other fields such as instrumentation development, molecular biology, surface chemistry, microfabrication, biological sample preparation and data handling. (blogspot.com)
  • This chapter provides details about the phytopathological disease diagnostics market based on test, and has been classified into molecular tests, serological tests, and test using electronic technology. (pharmiweb.com)
  • Siemens Healthineers applied compressed sensing technology to help solve a clinical problem: how to reduce long scan times while maintaining high diagnostic quality . (phys.org)
  • Previously, low diagnostic quality prevented these patient subsets from realizing any benefit from cardiac MRI, but the technology enables the entire cardiac cycle to be recorded in real time with only one breath-hold. (phys.org)
  • The smart Blood DNA Midi prep (a) is based on the unique SmartExtraction technology and allows an easy automation of DNA extraction from up to 3 ml of whole blood. (environmental-expert.com)
  • Technology of recombinant DNA and their importance for study of microorganisms. (muni.cz)
  • The multiplex structure and the process of forming it have diagnostic, therapeutic, prophylactic and nanoengineering applications. (google.com)
  • Light interaction with biomaterials is the driving mechanism of fundamental biological processes, from photosynthesis to DNA photodamage, and is a powerful tool in biomedicine for analytical, diagnostic and therapeutic purposes. (europa.eu)
  • Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. (abcam.com)
  • The future envisages a DNA probe, which offers the ability to detect complex interrelationships between multiple gene expression and environmental factors, at the molecular level. (prweb.com)
  • Diagnostic tests are also taking advantage of the small sizes, using probes of unusually shaped DNA that can detect cancer with remarkable accuracy. (scientificamerican.com)
  • Burgeoning Molecular Diagnostics Market Propels the Global DNA Probes-Based Diagnostics Market, According to New Report by Global Industry Analysts, Inc. (prweb.com)
  • GIA announces the release of a comprehensive global report on DNA Probe-Based Diagnostics markets. (prweb.com)
  • The report on "Global DNA Probe-based Diagnostic Market" is a professional report which provides thorough knowledge along with complete information pertaining to the DNA Probe-based Diagnostic industry a propos classifications, definitions, applications , industry chain summary, industry policies in addition to plans, product specifications, manufacturing processes, cost structures, etc. (openpr.com)
  • The report cloaks the market analysis and projection of "DNA Probe-based Diagnostic Market" on a regional as well as global level. (openpr.com)
  • The Global DNA Probe-based Diagnostic Market Research Report renders deep perception of the key regional market status of the DNA Probe-based Diagnostic Industry on a global level that primarily aims the core regions which comprises of continents like Europe, North America, and Asia and the key countries such as United States, Germany, China and Japan. (openpr.com)
  • It is a methodical research depending on the market and examines the competitive framework of the global DNA Probe-based Diagnostic Market industry. (openpr.com)
  • There are 14 Chapters to deeply display the global DNA Probe-based Diagnostic Profiler market. (sbwire.com)
  • Players, stakeholders, and other participants in the global DNA Probe-based Diagnostic market will be able to gain the upper hand as they use the report as a powerful resource. (reportsnreports.com)
  • Roche is a global pioneer in pharmaceuticals and diagnostics focused on advancing science to improve people's lives. (roche.com)
  • Also, U.S. and Europe together holds the majority of the global DNA Probes-based diagnostics market as about 80% to 90% of DNA tests are performed in the developed regions such as North America and Europe. (blogspot.com)
  • Global Phytopathological Disease Diagnostics Market on a. (pharmiweb.com)
  • The global phytopathological disease diagnostics is segmented in detail to cover every aspect of the market and present a complete market intelligence approach to the reader. (pharmiweb.com)
  • Dipsticks are one of the cheapest paper diagnostic products available in the global IVD market (parent market). (reportbuyer.com)
  • Pharmaceutical and biotechnology companies are one of the major market contributors to the global CTC diagnostics market. (reportbuyer.com)
  • Diba Industries creates and delivers precision fluid handling systems to global OEMs in diagnostics and medical devices. (jspargo.com)
  • The culminated effect of all of these variables will help in determining the efficacy of the technology's application in clinical diagnostics, in the years to come. (prweb.com)
  • POC test is one of the clinical diagnostics trending high in the H. pylori diagnostics and IVD markets. (reportbuyer.com)
  • High Heska Heska sells advanced veterinary diagnostic and specialty products. (reportbuyer.com)
  • Cancer Genetics Inc (CGI) is a pharmaceutical company that provides diagnostics products and services in oncology areas. (mynewsdesk.com)
  • The diagnostic assays and products offered by the company are designed to increase treatment efficacy and reduce healthcare costs of the hematological, urogenital and HPV-associated cancers. (sbwire.com)