Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Sensitivity and Specificity
Reagent Kits, Diagnostic
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
DNA Probes, HLA
DNA probes specific for the human leukocyte antigen genes, which represent the major histocompatibility determinants in humans. The four known loci are designated as A, B, C, and D. Specific antigens are identified by a locus notation and number, e.g., HLA-A11. The inheritance of certain HLA alleles is associated with increased risk for certain diseases (e.g., insulin-dependent diabetes mellitus).
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Evaluation Studies as Topic
In Situ Hybridization, Fluorescence
Molecular Probe Techniques
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Polymorphism, Restriction Fragment Length
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
RNA, usually prepared by transcription from cloned DNA, which complements a specific mRNA or DNA and is generally used for studies of virus genes, distribution of specific RNA in tissues and cells, integration of viral DNA into genomes, transcription, etc. Whereas DNA PROBES are preferred for use at a more macroscopic level for detection of the presence of DNA/RNA from specific species or subspecies, RNA probes are preferred for genetic studies. Conventional labels for the RNA probe include radioisotope labels 32P and 125I and the chemical label biotin. RNA probes may be further divided by category into plus-sense RNA probes, minus-sense RNA probes, and antisense RNA probes.
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Diagnostic Techniques and Procedures
Nucleic Acid Amplification Techniques
Sequence Analysis, DNA
Clinical Laboratory Techniques
Oligonucleotide Array Sequence Analysis
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Nucleic Acid Probes
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
Amino Acid Sequence
Microfluidic Analytical Techniques
False Positive Reactions
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Electrophoresis, Agar Gel
Sequence Homology, Nucleic Acid
Peptide Nucleic Acids
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Predictive Value of Tests
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
In Situ Hybridization
RNA, Ribosomal, 16S
Bacterial Typing Techniques
Repetitive Sequences, Nucleic Acid
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Shiga Toxin 1
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
False Negative Reactions
Enzyme-Linked Immunosorbent Assay
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A technique for identifying individuals of a species that is based on the uniqueness of their DNA sequence. Uniqueness is determined by identifying which combination of allelic variations occur in the individual at a statistically relevant number of different loci. In forensic studies, RESTRICTION FRAGMENT LENGTH POLYMORPHISM of multiple, highly polymorphic VNTR LOCI or MICROSATELLITE REPEAT loci are analyzed. The number of loci used for the profile depends on the ALLELE FREQUENCY in the population.
Sex Chromosome Aberrations
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Gene Expression Profiling
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Tumor Markers, Biological
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Chromosomes, Human, Pair 1
Highly repetitive DNA sequences found in HETEROCHROMATIN, mainly near centromeres. They are composed of simple sequences (very short) (see MINISATELLITE REPEATS) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct BASE COMPOSITION.
Substances that are toxic to cells; they may be involved in immunity or may be contained in venoms. These are distinguished from CYTOSTATIC AGENTS in degree of effect. Some of them are used as CYTOTOXIC ANTIBIOTICS. The mechanism of action of many of these are as ALKYLATING AGENTS or MITOSIS MODULATORS.
A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
Diagnostic Tests, Routine
Nucleic Acid Denaturation
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Mycobacterium avium Complex
A complex that includes several strains of M. avium. M. intracellulare is not easily distinguished from M. avium and therefore is included in the complex. These organisms are most frequently found in pulmonary secretions from persons with a tuberculous-like mycobacteriosis. Strains of this complex have also been associated with childhood lymphadenitis and AIDS; M. avium alone causes tuberculosis in a variety of birds and other animals, including pigs.
Deoxyribonucleases, Type II Site-Specific
Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 126.96.36.199.
The use of light interaction (scattering, absorption, and fluorescence) with biological tissue to obtain morphologically based information. It includes measuring inherent tissue optical properties such as scattering, absorption, and autofluorescence; or optical properties of exogenous targeted fluorescent molecular probes such as those used in optical MOLECULAR IMAGING, or nontargeted optical CONTRAST AGENTS.
The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level.
Reverse Transcriptase Polymerase Chain Reaction
So-called atypical species of the genus MYCOBACTERIUM that do not cause tuberculosis. They are also called tuberculoid bacilli, i.e.: M. buruli, M. chelonae, M. duvalii, M. flavescens, M. fortuitum, M. gilvum, M. gordonae, M. intracellulare (see MYCOBACTERIUM AVIUM COMPLEX;), M. kansasii, M. marinum, M. obuense, M. scrofulaceum, M. szulgai, M. terrae, M. ulcerans, M. xenopi.
Staining and Labeling
The branch of medicine concerned with the application of NANOTECHNOLOGY to the prevention and treatment of disease. It involves the monitoring, repair, construction, and control of human biological systems at the molecular level, using engineered nanodevices and NANOSTRUCTURES. (From Freitas Jr., Nanomedicine, vol 1, 1999).
Ligase Chain Reaction
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
Protein Array Analysis
Indicators and Reagents
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
Limit of Detection
Medical Laboratory Science
Oligonucleotides and Taq DNA polymerases for use in Molecular Diagnostic applications. Manufacturing processes take place in ... Real-time qPCR Probes, assay services, and proteomics. The proteomics operations are primarily concerned with custom peptides ... The in vitro diagnostics business unit provides technical and project support for contract manufacturing of good manufacturing ... 2008: Eurogentec received ISO 13485 Certification for the production and sales of In Vitro Diagnostics (IVD) oligonucleotides ...
FARMS has been designed for preprocessing and summarizing high-density oligonucleotide DNA microarrays at probe level to ... For targeted next-generation-sequencing panels in clinical diagnostics, in particular for cancer, Hochreiter's group developed ... A DNA segment is identical by state (IBS) in two or more individuals if they have identical nucleotide sequences in this ... cn.MOPS estimates the local DNA copy number, is suited for both whole genome sequencing and exom sequencing, and can be applied ...
Karen Joy Shaw
... leading to the development of over 20 DNA diagnostic probes for evaluating aminoglycoside resistance in clinical isolates. ... Aminoglycoside resistance/diagnostics. As a leader in the field of aminoglycoside resistance Shaw was involved in the cloning ... Novel DNA Gyrase inhibitors. At Trius Pharmaceuticals, Shaw lead the microbiology efforts in designing a screening regimen for ... Morrow, Brian J.; Shaw, Karen Joy (2002), "DNA Microarray Expression Analysis in Antibacterial Drug Discovery", Pathogen ...
The HyX platform had five core technologies: Labeled DNA probes DNA arrays of samples and probes Software-driven modules like ... Hyseq's genomic platform was used to develop gene based therapeutics and diagnostics. At the rate of more than 1,000,000 human ... HyGnostics and HyChip Industrial robots to screen DNA probes Bioinformatics software for information management Applications ... Nuvelo was initially founded as Hyseq in August 1992 and based on DNA array technology. ...
Centrifugal micro-fluidic biochip
... the target molecules are labeled with fluorescent tags and this biotin-labeled target DNA is hybridized with the probe DNA ... His group members are working on projects including cell lysis, PCR card, DNA hybridization, anthrax diagnostics and ... the probe molecules (biotin, DNA, or human plasma IgG) are covalently attached to the polycarbon surface via amide coupling ( ... is used to amplify the target genetic marker with the DNA-binding fluorescence dye or a sequence-specific probe is applied for ...
... in the DNA. Some examples of molecular diagnostic tests include DNA sequencing and Southern blotting.. College (4 yrs) → ... Fluorescence in situ hybridization (FISH) involves fluorescent labeling of probes that bind to specific DNA sequences, used for ... DNA sequencing is used to directly analyze the genomic DNA sequence of a particular gene. In general, only the parts of the ... Diagnostic evaluation. Each patient will undergo a diagnostic evaluation tailored to their own particular presenting ...
Indian Institute of Horticultural Research
Nucleic acid probes for many viruses. DNA fingerprinting techniques for characterization and documentation of germplasm. ... Development of Diagnostics and to offer diagnostic services to SAUs, Farmers and Private seed and Tissue culture industries. ... Diagnostics : Diagnosis of diseases of various horticultural crops and advice on their control and management. Supply of Video ... Integrated disease management protocols and diagnostic kits for viruses. Post Harvest Technology : IIHR has contributed to the ...
Multiplex ligation-dependent probe amplification
Each probe consists of two oligonucleotides which recognize adjacent target sites on the DNA. One probe oligonucleotide ... Identification of deletions or duplications can indicate pathogenic mutations, thus MLPA is an important diagnostic tool used ... The signal strengths of the probes are compared with those obtained from a reference DNA sample known to have two copies of the ... If the probes were not split in this way, the primer sequences at either end would cause the probes to be amplified regardless ...
Dual Hybridization (LightCycler®) probes Scorpions® Probes LUX (Light Upon Extension) Probes DNA binding dye assays (e.g., SYBR ... nucleic acid detection Real-time PCR quantification Allelic discrimination and identification Multiplex PCR assays Diagnostic ... Molecular beacons, or molecular beacon probes, are oligonucleotide hybridization probes that can report the presence of ... Fluorogenic signaling oligonucleotide probes were reported for use to detect and isolate cells expressing one or more desired ...
... negatively charged DNA and molecular probes can be concentrated on energized electrodes for interaction Using photolithography ... Some of its major applications include genomics, proteomics, molecular diagnostics, point-of-care diagnostics, tissue ... The reaction involves thermal cycling of the DNA sequence and DNA polymerase through three different temperatures. Heating up ... Michigan probes have been used in large-scale recordings and network analysis of neuronal assemblies , and the Utah electrode ...
It consists of fluorescently labeled DNA probes to the pericentromeric regions of chromosomes 3 (red), 7 (green), and 17 (aqua ... Abbott Moledular: UroVysion Bladder Cancer Kit For In Vitro Diagnostic Use v t e v t e. ...
Comparative genomic hybridization
... with these fragments being used as probes on the DNA microarray. Now probes of various origins such as cDNA, genomic PCR ... making it far more adaptable to diagnostic uses. The DNA on the slide is a reference sample, and is thus obtained from a ... repetitive DNA regions like centromeres and telomeres need to be blocked with unlabeled repetitive DNA (e.g. Cot1 DNA) and/or ... DNA from the sample to be tested is labeled with a red fluorophore (Cyanine 5) and a reference DNA sample is labeled with green ...
DNA read errors
... complex simultaneous probe hybridization and direct mutation detection to help solve the dual problem of multiple sample ... developed a new approach that provided a breakthrough in dealing with bubbles using the multiplex allele-specific diagnostic ... In bioinformatics, a DNA read error occurs when a sequence assembler changes one DNA base for a different base. The reads from ... Mechanisms of DNA Recombination and Genome Rearrangements: Intersection Between Homologous Recombination, DNA Replication and ...
Sensors may pick up on abnormalities with DNA as well as the presence of antibodies. An ultrasonic probe can plug into a ... There are reports that medical tricorders may emerge from "diagnostic medical apps" via Tablet Computers and smartphones. Some ... This British biotech firm is developing a device described as a "handheld DNA lab" to analyze malaria; the firm is raising ... It developed a virus detection device called Q-POC which breaks open cells to analyze their DNA. Ibis Biosciences. This firm ...
Loop-mediated isothermal amplification
The use of a strand-displacing DNA polymerase in LAMP also precludes the use of hydrolysis probes, e.g. TaqMan probes, which ... In a diagnostic application, this must be balanced against the need to choose an appropriate target (e.g., a conserved site in ... gene using loop-mediated isothermal amplification combined with DNA functionalized gold nanoparticles as probes". Aquaculture. ... likely due to use of a different DNA polymerase (typically Bst - Bacillus stearothermophilus - DNA polymerase rather than Taq ...
... has been used to study DNA-protein interactions (using a terbium chelate complex) to measure distances in DNA ... Traditional virus diagnostic procedures are being replaced by sensitive immunoassays with lanthanides. The time resolved ... Lanthanide probes ligands must meet several chemical requirements for the probes to work properly. These qualities are: water ... Several systems have been proposed which combine MRI capability with lanthanides probes in dual assays. The luminescent probe ...
For the diagnostics of beta-thalassemia mutations, a simple chip was designed that contained six probes corresponding to ... By performing this reaction at a temperature above the melting temperature of the duplex between the DNA and immobilized probe ... A set of potential hybridization probes are created for each DNA sequence that form perfect duplexes with that sequence. The ... probes) onto these gel pads. Test sample is then added to this micromatrix containing immobilized probes in gel pads and ...
Laboratory diagnosis of viral infections
In the diagnostic laboratory virus infections can be confirmed by a multitude of methods. Diagnostic virology has changed ... Detection of viral RNA and DNA genomes can be performed using polymerase chain reaction. This technique makes many copies of ... the virus genome using virus-specific probes. Variations of PCR such as nested reverse transcriptase PCR and real time PCR can ... Serology Molecular diagnostics Leland, D. S.; Ginocchio, C. C. (2007). "Role of Cell Culture for Virus Detection in the Age of ...
DAPI is a dye that binds to DNA and fluoresces blue. For this reason, it is particularly useful at labeling nuclei. Antibody ... However, the increased rate of binucleation is usually not high enough to make it a conclusive diagnostic tool. The fate of ... probes can be used to label tubulin fluorescently. The immunofluorescence may then be observed with microscopy. Binucleated ...
... on which pieces of DNA (probes) are affixed in a microscopic array. Similar to a DNA microarray, a protein array is a miniature ... Yetisen A. K. (2013). "Paper-based microfluidic point-of-care diagnostic devices". Lab on a Chip. 13: 2210. doi:10.1039/ ... DNA chips (microarrays). Early biochips were based on the idea of a DNA microarray, e.g., the GeneChip DNAarray from ... Microfluidics emerged in the beginning of the 1980s and is used in the development of inkjet printheads, DNA chips, lab-on-a- ...
Hereditary fructose intolerance
In HFI, the diagnosis of homozygotes is difficult, requiring a genomic DNA screening with allele specific probes or an enzyme ... avoid testing via fructose challenge as the results are non-conclusive for individuals with HFI and even if the diagnostic ...
In situ hybridization - Specific DNA and RNA molecules can be identified on sections using this technique. When the probe is ... In others, they are in essence clinician group practices that employ pathologists to provide diagnostic services for the group ... all in addition to the practice of diagnostic anatomical pathology. Pathologists in academic settings often sub-specialize in a ... will control a partnership that employs junior pathologists and contracts independently with hospitals to provide diagnostic ...
Fluorescence in situ hybridization
Probe size is important because longer probes hybridize less specifically than shorter probes, so that short strands of DNA or ... it may lead to more portable diagnostic techniques. ... Probes - RNA and DNA. ViewRNA detection of miR-133(green ... Repetitive DNA sequences must be blocked by adding short fragments of DNA to the sample. The probe is then applied to the ... Preparing DNA probes for one species and performing FISH with this probe allows one to visualize the distribution of this ...
Early in the 1970s, they exploited Avidin as a probe and developed new methods and reagents to biotinylate antibodies and other ... Today, the system is applied in research and diagnostics as well as medical devices and pharmaceuticals. Examples include ... DNA, hormone, etc.) without disturbing the interaction with its target molecule; avidin is then used to "sandwich" between the ... Consequently, the avidin-biotin system can frequently replace radioactive probes. Together with Ed Bayer, Wilchek established ...
SNP stands for Single nucleotide polymorphism probes. These probes allow for increased precision and greater diagnostic yield. ... prenatal and pediatric diagnostics, offering DNA-based testing for the detection of genetic abnormalities beyond what can be ... and opened a diagnostics lab in Irvine, CA, which was originally led by former US Labs employees with the intent of entering ... The median spatial resolution between probes is 1 Kb within gene rich regions and 5 Kb outside of gene-rich regions. Lash, Alex ...
C. immitis and C. posadasii cannot be distinguished on cytology or by symptoms, but only by DNA PCR. An indirect demonstration ... Research demonstrates that patients from endemic areas who are aware of the disease are most likely to request diagnostic ... It can also be detected in culture by morphological identification or by using molecular probes that hybridize with C.immitis ... The fungal infection can be demonstrated by microscopic detection of diagnostic cells in body fluids, exudates, sputum and ...
Locked nucleic acid
Conversely, LNA-incorporated probes demonstrated increased hybridization efficiency in both DNA and RNA. The improved ... Due to its high affinity for mismatch discrimination, LNA has been studied for its applications in diagnostic tools. ... LNA nucleotides can be mixed with DNA or RNA residues in the oligonucleotide, in effect hybridizing with DNA or RNA according ... "Improved in situ hybridization efficiency with locked-nucleic-acid-incorporated DNA probes". Applied and Environmental ...
... a capability key to various diagnostic and therapeutic protocols. Breslauer has mapped the energetic consequences of DNA damage ... The database and its extensions also have been used to design probes and drugs with predictable hybridization and binding ... At Rutgers since 1974, he combines these fields by investigating protein-DNA, and drug-DNA interactions, particularly as they ... PREDICTING DNA DUPLEX STABILITY FROM THE BASE SEQUENCE, KJ Breslauer et al., PNAS Vol 83, pp 3746-3750, June 1986[permanent ...
Luc Van den hove
"Imec releases neuropixels neural probe to the global Neuroscience Community". www.imec-int.com. "Imec tool accelerates DNA ... "Imec awarded NASA grant to advance space health diagnostics". www.newelectronics.co.uk. "The programmable brain-on-a-chip". www ... In 2017, IMEC designed and fabricated the world's first miniature neural probe for simultaneous recording of multiple brain ... neural probes, lab-on-chip, organ-on-chip), wireless communication (e.g. 5G and wireless IoT communication), image sensors, and ...
In DNA-based diagnostic tests, complexes are used to determine whether electrons can flow across the DNA. If an electron could ... By using specially-designed chiral metal complexes as probes of DNA she has been able to study how DNA is damaged and repaired ... Her experiments reveal a strategy for how DNA repair proteins locate DNA lesions and demonstrate a biological role for DNA- ... This view of DNA is deeper and more dynamic than previous views of DNA as a static library. Understanding the mediation of ...
Molecular and Cellular Probes. 31: 22-27. doi:10.1016/j.mcp.2016.08.003. PMID 27523487. Archived (PDF) from the original on 20 ... Diagnostic method. Based on symptoms, tick exposure, blood tests. Prevention. Prevention of tick bites (clothing the limbs, ... The examination of preserved museum specimens has found Borrelia DNA in an infected Ixodes ricinus tick from Germany that dates ... Except for one study in Europe, much of the data implicating lizards is based on DNA detection of the spirochete and has ...
Oxygen radical absorbance capacity
Fluorescein is currently used most as a fluorescent probe. Equipment that can automatically measure and calculate the capacity ... and protection of DNA, proteins and lipids from oxidative damage pursuant to Article 13(1) of Regulation (EC) No 1924/20061". ... is commercially available (Biotek, Roche Diagnostics). The fluorescent intensity decreases as the oxidative degeneration ... "Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe ...
Epigenetics of neurodegenerative diseases
These results, found by probing with immunoassays, have been challenged by studies that interrogate DNA sequence by bisulfite ... This DNA-histone structure is called a nucleosome; the more tightly the DNA is bound by the nucleosome, and the more tightly a ... In mammals, methylation occurs on DNA and histone proteins. DNA methylation occurs on the cytosine of CpG dinucleotides in the ... DNA methylation. In Alzheimer's disease cases, global DNA hypomethylation and gene-specific hypermethylation has been observed ...
Surface-enhanced Raman spectroscopy
New Probe Detects Trace Pollutants in Groundwater". Oak Ridge National Laboratory Review. 26 (2).. ... SERS can be used to target specific DNA and RNA sequences using a combination of gold and silver nanoparticles and Raman-active ... fundamental aspects and diagnostic applications". Analytical and Bioanalytical Chemistry. 407 (27): 8265-8277. doi:10.1007/ ... The gold nanoparticles facilitate the formation of a silver coating on the dye-labeled regions of DNA or RNA, allowing SERS to ...
quantitative PCR does not require this, as the detection system uses fluorescence and probes to detect the DNA molecules as ... Diagnostic tests. Main article: Diagnostic microbiology. Identification of an infectious agent for a minor illness can be ... This would prove invaluable for diagnostic techniques. Commonly treated infectious diseases. Bacterial. * ... DNA sequencing, a method developed by Walter Gilbert and Frederick Sanger in 1977, caused a rapid change the development of ...
Using RT-PCR, diagnostic results can be available in one to two days. Serological diagnosis requires a larger amount of ... a nonstructural protein that degrades RBP1 and turns off the host cell's ability to transcribe DNA. NS2 interferes with the ... "Probing the attenuation and protective efficacy of a candidate chikungunya virus vaccine in mice with compromised interferon ( ...
Üksiku nukleotiidi polümorfism - Vikipeedia, vaba entsüklopeedia
"Standard Mutation Nomenclature in Molecular Diagnostics" The Journal of Molecular Diagnostics 9 (1), 2007 ... Kaasaegsete andmete kohaselt erineb kahe eri inimese DNA kuni 0,1% ehk vähem kui 3 miljonit DNA aluspaari. Erinevates ... "Genotyping of triallelic SNPs using TaqMan® PCR" Molecular and Cellular Probes 21 (3): 171-176. ... DNA molekul 1 erineb DNA molekulist 2 ühe aluspaari juures (C/T polümorfism). ...
Green signal from anti-tubulin antibody conjugated with Alexa Fluor 488) and nuclei (blue signal from DNA stained with DAPI) in ... CLSM has the advantage of not requiring a probe to be suspended nanometers from the surface, as in an AFM or STM, for example, ... Medical diagnostics. MeSH. D018613. OPS-301 code. 3-301. [edit on Wikidata] ...
"Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA". Electrophoresis. National Diagnostics. Retrieved 13 October 2016. ... Studies comparing different denaturation methods such as heating, beads mill of different bead sizes, probe sonification, and ... DNA repair or protein binding are set to occur. The area of partially separated DNA is known as the denaturation bubble, ... Melting curves of DNA". doi:10.2172/5693881.. *^ López-Alonso JP, Bruix M, Font J, Ribó M, Vilanova M, Jiménez MA, Santoro J, ...
... also called DNA probes, to reconstruct a target DNA sequence. Non-specific hybrids are removed by washing and the target DNA is ... "The Journal of Molecular Diagnostics. 10 (6): 484-92. doi:10.2353/jmoldx.2008.080027. PMC 2570630 . PMID 18832462.. ... large DNA fragments into shorter DNA fragments. The fragmented DNA may then be cloned into a DNA vector and amplified in a ... DNA nanoball sequencing. Main article: DNA nanoball sequencing. DNA nanoball sequencing is a type of high throughput ...
... cells grown in culture and stained with antibody to tubulin (green), antibody to Ki-67 (red) and the blue DNA binding dye ... Dual-color FISH with a c-MYC probe mapping to 8q24 revealed colocalization with HPV18 at all integration sites, indicating that ... heptamethine dyes IR-808 and other analogs which are currently being explored for their unique uses in medical diagnostics, the ... Human papillomaviruses (HPVs) are frequently integrated into the cellular DNA in cervical cancers. We mapped by FISH five HPV18 ...
The filter and colonies are prepared for hybridization and then labeled with a probe. The target DNA- insert of interest- can ... Such genome-wide assessments may lead to further diagnostic and drug therapies while also helping future teams focus on ... The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a ... Use the enzyme DNA ligase to seal the DNA fragments into the vector. This creates a large pool of recombinant molecules. These ...
Polzer et al, EMBO 2014, Molecular profiling of single Circulating Tumor cells with diagnostic intention Polzer Et all EMBO ... DNA, chromosomes, proteins and viruses. DEP can be used to separate particles with different sign polarizabilities as they move ... thus when probed with a light beam the well the light intensity increases through the well. The opposite is true for negative ... DEP is being applied in fields such as medical diagnostics, drug discovery, cell therapeutics, and particle filtration. ...
... thus allowing the use of small exogenous molecules as biomolecular probes. A fluorophore can be attached to one of these probes ... Rieder, Ulrike; Luedtke, Nathan W. (25 August 2014). "Alkene-tetrazine ligation for imaging cellular DNA". Angew Chem Int Ed ... Integrated Diagnostics, and the biotech company baseclick, a BASF spin-off created to sell products made using click ... Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called ...
... found in DNA upstream of each constant region gene (except in the δ-chain). The DNA strand is broken by the activity of a ... Goding J (1978). "Allotypes of IgM and IgD receptors in the mouse: a probe for lymphocyte differentiation". Contemp Top ... Detection of particular antibodies is a very common form of medical diagnostics, and applications such as serology depend on ... RAG proteins play an important role with V(D)J recombination in cutting DNA at a particular region. Without the presence of ...
ATP-binding cassette transporter
This diagnostic fold can also be found in the MalF and MalG TM subunits of MalFGK2 and the Met transporter MetI. In the MetI ... The third subgroup of ABC proteins do not function as transporters, but are rather involved in translation and DNA repair ... the modulation of the accumulation of a probe substrate (e.g. fluorescent dyes like rhodamine 123, or calcein) can be ... The name ATP-binding cassette is derived from the diagnostic arrangement of the folds or motifs of this class of proteins upon ...
One notable use is diagnostic X-ray imaging in medicine (a process known as radiography). X-rays are useful as probes in high- ... UV rays in the middle range can irreparably damage the complex DNA molecules in the cells producing thymine dimers making it a ... gamma rays are used for diagnostic imaging in nuclear medicine, an example being PET scans. The wavelength of gamma rays can be ...
Two probes must be used for each SNP position to detect both alleles; if only one probe were used, experimental failure would ... The basic principles of SNP array are the same as the DNA microarray. These are the convergence of DNA hybridization, ... Sheils, O; Finn, S; O'Leary, J (2003). "Nucleic acid microarrays: An overview". Current Diagnostic Pathology. 9 (3): 155-8. doi ... In molecular biology, SNP array is a type of DNA microarray which is used to detect polymorphisms within a population. A single ...
In May 2015, Pfizer and a Bar-Ilan University laboratory announced a partnership based on the development of medical DNA ... "Congress joins probe into Wyeth's Rapamune marketing". FiercePharma. Retrieved July 11, 2011 ... "Pfizer to collaborate on Bar-Ilan DNA robots". Globes. Retrieved June 6, 2015 ...
"Proteomics of mouse BRCA1-deficient mammary tumors identifies DNA repair proteins with potential diagnostic and prognostic ... Multiplex Ligation-dependent Probe Amplification (MLPA), and Quantitative Multiplex PCR of Short Fluorescent Fragments ( ... If DNA repair is deficient, DNA damage tends to accumulate. Such excess DNA damage may increase mutational errors during DNA ... BRCA1 directly binds to DNA, with higher affinity for branched DNA structures. This ability to bind to DNA contributes to its ...
మానవ పాపిల్లోమా వైరస్ - వికీపీడియా
"gen-probe.com. Archived from the original on 12 December 2012.. *↑ 107.0 107.1 Dunne EF, Nielson CM, Stone KM, Markowitz LE, ... HPV DNA హైబ్రిడైజింగ్ HPV DNA ను కిట్ నుండి పూర్తిస్థాయి RNA సన్నివేశాలతో క్లినికల్ నమూనాల నుండి గుర్తించడం కోసం ఇది ఒక ణొన్ ... "Chapter 5: Technological Accretion in Diagnostics: HPV Testing and Cytology in Cervical Cancer Screening". మెడికల్ ఇన్నోవేషన్ ... లో వైరస్ యొక్క ఒకటి లేదా అంతకంటే ఎక్కువ జాతుల DNA ను కనుగొన్నారు. ఏది ఏమయినప్పటికీ, రక్తములో HPV DNA యొక్క ఉనికిని ...
sensitive non-radioactive in situ hybridization probes to detect nucleic acids in plants, able to detect 1 µg of plasmid DNA.[6 ... 2002). DIG Application Manual for Nonradioactive in situ Hybridization (3rd ed.). Penzberg: Roche Diagnostics.. ...
Clostridioides difficile infection
Diagnostic method. Stool culture, testing for the bacteria's DNA or toxins. ... "Scientists probe whether C. difficile is linked to eating meat". CBC News. 4 October 2006. Archived from the original on 24 ... The appearance of "pseudomembranes" on the mucosa of the colon or rectum is highly suggestive, but not diagnostic of the ... Stool leukocyte measurements and stool lactoferrin levels also have been proposed as diagnostic tests, but may have limited ...
DNA sequencing theory. References. *^ a b c Staden, R (1979). "A strategy of DNA sequencing employing computer programs" ... The end of one of these clones can then be sequenced to yield a new probe and the process repeated in a method called ... 2009). "Next Generation Sequencing: From Basic Research to Diagnostics". Clinical Chemistry. 55 (4): 41-47. doi:10.1373/ ... In genetics, shotgun sequencing is a method used for sequencing long DNA strands. It is named by analogy with the rapidly ...
Polymerase chain reaction
Detection of DNA using these methods can only be seen after the hybridization of probes with its complementary DNA takes place ... The primers used must be specific to the targeted sequences in the DNA of a virus, and PCR can be used for diagnostic analyses ... including a DNA template that contains the DNA target region to amplify; a DNA polymerase; an enzyme that polymerizes new DNA ... Digital PCR (dPCR): used to measure the quantity of a target DNA sequence in a DNA sample. The DNA sample is highly diluted so ...
2008 Noida double murder case
... and director of the Centre for DNA Fingerprinting and Diagnostics, which conducted the DNA testing for the Aarushi case). ... "Deputation of CBI officer probing Noida case ends". The Times of India. 2 July 2008.. ... The CBI then sent the sample for further testing to the Centre for DNA Fingerprinting and Diagnostics (CDFD) in Hyderabad. The ... The kukri was then sent to the CFSL DNA expert BK Mahapatra, who said he could not extract any DNA from the weapon. ...
Burgeoning Molecular Diagnostics Market Propels the Global DNA Probes-Based Diagnostics Market, According to New Report by...
In the medical diagnostics laboratories of the 21st century, the ability to detect nucleic ... the potential of DNA probes-based diagnostics is huge. DNA probes also render themselves as a powerful tool for chromosomal ... Roche Diagnostics, Siemens Healthcare Diagnostics, among others.. The research report titled "DNA Probes-Based Diagnostics: A ... Burgeoning Molecular Diagnostics Market Propels the Global DNA Probes-Based Diagnostics Market, According to New Report by ...
Global DNA Probe-based Diagnostic Market 2017-2021
About DNA Probe-based Diagnostic. The DNA probe is a basic component in most molecular diagnostic techniques. The DNA probe is ... Global DNA probe-based diagnostics market by infectious disease testing. *Global DNA probe-based diagnostics market by cancer ... Exhibit 16: DNA probe-based diagnostics market in EMEA 2016-2021 ($ billions). Exhibit 17: DNA probe-based diagnostics market ... Exhibit 19: DNA probe-based diagnostics market in APAC 2016-2021 ($ billions). Exhibit 20: DNA probe-based diagnostics market ...
The 2013-2018 World Outlook for DNA Probes-Based Diagnostics
This econometric study covers the world outlook for DNA probes-based - Market research report and industry analysis - 7785572 ... The 2013-2018 World Outlook for DNA Probes-Based Diagnostics The 2013-2018 World Outlook for DNA Probes-Based Diagnostics ... The 2013-2018 World Outlook for DNA Probes-Based Diagnostics. This econometric study covers the world outlook for DNA probes- ... Life Sciences»Biotechnology»DNA Sequencing The 2013-2018 World Outlook for DNA Probes-Based Diagnostics. Lowest Prices ...
Rhodium-conjugate fluorescent probes for diagnostic of mismatched DNA - CaltechAUTHORS
Nano, Adela and Barton, Jacqueline (2016) Rhodium-conjugate fluorescent probes for diagnostic of mismatched DNA. In: 252nd ... probes capable of detecting and signaling DNA base mismatches directly would be a powerful tool to this end. Previous studies ... In healthy cells, DNA base pair mismatches, as well as some other base pair anomalies derived from DNA base damage, are cor. ... measurements in the presence of well matched and mismatched DNA support the notion that our systems are interesting probes for ...
Reportlinker Adds Molecular Diagnostics Market: DNA Probes and Biochips -- New Product Development Opportunities and Business...
Diagnostics,Market:,DNA,Probes,and,Biochips,--,New,Product,Development,Opportunities,and,Business,Expansion,Strategies,for, ... The molecular diagnostics market is unquestionably the most rapidly gr...,Reportlinker,Adds,Molecular, ... Blood Supply, Tissue Banking and Transplantation Industry: Molecular Diagnostics Market: DNA Probes and Biochips -- New Product ... Reportlinker Adds Molecular Diagnostics Market: DNA Probes and Biochips -- New Product Development Opportunities and Business ...
Diagnostic efficacy of a DNA probe in pneumonia caused by Legionella species
Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing Legionnaires disease in 167 ... Diagnostic efficacy of a DNA probe in pneumonia caused by Legionella species J Med Microbiol. 1993 Mar;38(3):183-6. doi: ... A commercial DNA probe kit (Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing ... The diagnostic performance of the DNA probe assay in this study was superior to that of the DFA test. The results indicate that ...
DNA Probe-based Diagnostic Market Growth, Key Futuristic, Future Opportunities and Forecast from 2017 to 2022 - openPR
DNA Probe-based Diagnostic Market Growth, Key Futuristic, Future Opportunities and Forecast from 2017 to 2022 - published on ... 1 Industry Overview of DNA Probe-based Diagnostic. 1.1 DNA Probe-based Diagnostic Market Overview. 1.1.1 DNA Probe-based ... 1.3 DNA Probe-based Diagnostic Market by Type. 1.3.1 DNA Probes-Based Products. 1.3.2 Direct Detection of Target Sequence. 1.3. ... Gene Detection Using DNA Chips. Gene Detection by DNA Sequencing. Market segment by Application, DNA Probe-based Diagnostic can ...
DNA Probe-Based Diagnostic Market Set for Rapid Growth 2018-2025 | Apr 16, 2018 - ReleaseWire
The latest market intelligence study on DNA Probe-based Diagnostic market relies on statistics derived from the application of ... opportunities and competitive landscape of DNA Probe-based Diagnostic market for the forecast period, 2018 - 2025. Importantly ... DNA Probe-Based Diagnostic Market Set for Rapid Growth 2018-2025. DNA Probe-based Diagnostic Market report helps to increase ... Scope of DNA Probe-based Diagnostic Market Research Report-. In addition, the research on the DNA Probe-based Diagnostic market ...
The 2016 Report on DNA Probes-Based Diagnostics: World Market Segmentation by City
World Market Segmentation by City The 2016 Report on DNA Probes-Based Diagnostics: World Market Segmentation by City This ... Life Sciences»Diagnostics»In Vitro Diagnostics»Molecular Diagnostics The 2016 Report on DNA Probes-Based Diagnostics: World ... The 2016 Report on DNA Probes-Based Diagnostics: World Market Segmentation by City. This report was created for global ... In what follows, I summarize the economic potential for the worlds major cities for "DNA probes-based diagnostics" for the ...
Content - DNA Probes-Based Diagnostics 2015 - 2022 | MCP-3405
This report analyzes the worldwide markets for DNA Probes-Based Diagnostics in US$ Million by the following Application Areas: ... What are DNA-Probes?. DNA-based Diagnostics - A Prelude. DNA Tests: Finding Use in Numerous Applications. DNA Probes: Robust ... DNA Chips. Chromosome Painting Probes. Diagnostic Applications Based on DNA Probes: An Overview. Infectious Diseases. ... 3.DNA PROBES-BASED DIAGNOSTICS - A PRIMER. Diagnostic Gene Detection Products. Molecular Diagnostics: A Powerful Disease ...
Global and Japan DNA Probe-based Diagnostic Market Insights, Forecast to 2026 : ReportsnReports
DNA Probe-based Diagnostic market is segmented by region (country),... ... 130 Pages Report] Check for Discount on Global and Japan DNA Probe-based Diagnostic Market Insights, Forecast to 2026 report by ... revenue generated in DNA Probe-based Diagnostic business, the date to enter into the DNA Probe-based Diagnostic market, DNA ... U.A.E DNA Probe-based Diagnostic Sales Growth Rate (2015-2020) (K Units). Figure 106. U.A.E DNA Probe-based Diagnostic Revenue ...
Roche NimbleGen Launches 2.1 Million-Probe Arrays for the Detection of Chromosomal Aberrations and DNA Copy Number Variation,...
Roche Diagnostics Deutschland GmbH, Roche NimbleGen, a company of Roche announced today the launch of comparative genomic ... Roche Diagnostics Deutschland GmbH * BoxId: 209139 - Roche NimbleGen Launches 2.1 Million-Probe Arrays for the Detection of ... Roche NimbleGen Launches 2.1 Million-Probe Arrays for the Detection of Chromosomal Aberrations and DNA Copy Number Variation. ... Recommend Roche NimbleGen Launches 2.1 Million-Probe Arrays for the Detection of Chromosomal Aberrations and DNA Copy Number ...
In situ hybridization with biotinylated DNA probes: a rapid diagnostic test for adenovirus upper respiratory infections. -...
Adenoviruses of subgroups B, C and E were detected equally well by probes prepared either from purified adenovirus type 5 or ... The use of stable non-radioactive probes makes in situ hybridization a feasible assay for use in clinical laboratories with ... Adenovirus DNA was detected in cells from nasopharyngeal secretions of children with acute respiratory infections by in situ ... hybridization with biotinylated probes. The technique was easy to perform, giving rapid results which were well correlated with ...
Worldwide Outlook on the Commercial Laundry Machinery Market to 2024: Washers, Commercial Dryers, Presses, and Dry Cleaning...
Patent US6315958 - Flow cell for synthesis of arrays of DNA probes and the like - Google Patents
A flow cell which can be used in the synthesis of DNA probes on an active surface of the substrate includes a base having a ... Methods for producing uniquely specific nucleic acid probes. WO2011128096A1. Apr 14, 2011. Oct 20, 2011. Roche Diagnostics Gmbh ... A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:. (a) a base having ... 1. A flow cell of the type that may be used in the synthesis of arrays of DNA probes and the like, comprising:. (a) a base ...
United States DNA/RNA Probe Diagnostic System Markets Market Research
... the Market for DNA/RNA Probe Diagnostic Systems Identification of Challenges Facing the Market for DNA/RNA Probe Diagnostic ... and Market SegmentationIntroduction to DNA/RNA Probe Diagnostic Systems MarketIntroduction to DNA/RNA Probe Diagnostic Systems ... United States DNA/RNA Probe Diagnostic System Markets 22-Feb-1999 / Research Code: 5847-01-00-00-00 / SKU: HC00280-NA-MR_03295 ... United States DNA/RNA Probe Diagnostic System Markets, Executive SummaryObjectives and PurposeObjectives and PurposeReport ...
Dermatology Deals & Contracts in Healthcare Sector Reviewed in New Topical Report Available at MarketPublishers.com
Global DNA Probes-Based Diagnostic Market Research Report Forecast 2017 to 2021 Research N Reports
Global DNA Probes-Based Diagnostic Market Research Report Forecast 2017 to 2021 includes market share, market research report, ... Chapter 1 DNA Probes-Based Diagnostic Market Overview.  1.1 Product Overview and Scope of DNA Probes-Based Diagnostic. & ... nbsp3.5.1 DNA Probes-Based Diagnostic Market Concentration Rate.  3.5.2 DNA Probes-Based Diagnostic Market Share of Top 3 ... nbsp6.3 Global DNA Probes-Based Diagnostic Price by Type (2012-2017).  6.4 Global DNA Probes-Based Diagnostic Production ...
DNA Probes for Identifying the Members of the Anopheles punctulatus Complex in Papua New Guinea | The American Journal of...
Specific DNA probes were developed for An. punctulatus, An. koliensis, and three sibling species, An. farauti No. 4, An. ... 6, by differentially screening total genomic DNA libraries of individual species and sibling species with homologous DNA ... Probes ranged from 273 to 630 bp. Identification of species can be made from squash or dot blots using only a segment of the ... Abstract Genomic DNA probes were made for five members of the Anopheles punctulatus complex of mosquitoes found in Papua New ...
Invader probes: harnessing the energy of intercalation to facilitate recognition of chromosomal DNA for diagnostic applications
Roche - Roche launches new VENTANA HER2 Dual ISH companion diagnostic test for breast and gastric cancer patients eligible for...
About the VENTANA HER2 Dual ISH DNA Probe Cocktail assay. Roche is the only provider of a fully automated brightfield Dual ISH ... Roche (SIX: RO, ROG; OTCQX: RHHBY) today announced the launch of the new VENTANA HER2 Dual ISH DNA Probe Cocktail assay for the ... The VENTANA HER2 Dual ISH DNA Probe Cocktail assay3 aids in identifying breast and gastric cancer patients eligible for the ... The VENTANA HER2 Dual ISH DNA Probe Cocktail assay is designed to be completed within the same day, providing clinicians the ...
Diagnostic efficacy of a DNA probe in pneumonia caused by Legionella species | Microbiology Society
The diagnostic performance of the DNA probe assay in this study was superior to that of the DFA test. The results indicate that ... Depending on the diagnostic criteria, the probe test had a sensitivity of 31-67%, a specificity of 99% and positive predictive ... The probe assay result was positive in six patients; five of them had other laboratory evidence of disease (positive cultures ... Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing Legionnaires' disease ...
Researchers find way to ID many pathogens with few DNA probes
... quickly and at low cost using a single set of random DNA probes. Rices universal microbial diagnostic, ... ... Universal microbial diagnostics using random DNA probes, Science Advances (2016). DOI: 10.1126/sciadv.1600025 ... If the DNA from a patients sample binds with the complementary DNA in a disease probe, the diagnosis is positive for that ... quickly and at low cost using a single set of random DNA probes. Rices "universal microbial diagnostic," or UMD, uses pieces ...
DNA Probes for the Identification of Pathogenic Foodborne Bacteria and Viruses | SpringerLink
Diagnostic deoxyribonucleic acid probes for infectious diseases. Clin. Microbiol. Rev. 1:82-101.PubMedPubMedCentralCrossRef ... Molecular cloning of a species-specific DNA probe for Campylobacter jejuni. Mol. Cell. Probes 1:245-259.PubMedCrossRefGoogle ... DNA probes assays for detection of Campylobacter and Salmonella. In Nucleic Acid and Monoclonal Antibody Probes, ed. B. ... Development of a Salmonella-specific biotinylated DNA probe for rapid routine identification of Salmonella. Mol. Cell. Probes 2 ...
Oligonucleotide Synthesis Market by Product (Primer, Probe, Linker, Adaptor, Custom, Reagent, Equipment), Application (PCR, DNA...
7.3 Diagnostics. 7.4 Therapeutics. 7.4.1 RNAi. 7.4.2 Nucleic Acid Aptamers. 7.4.3 DNA/Antisense Oligonucleotides. 7.4.4 ... Oligonucleotide Synthesis Market by Product (Primer, Probe, Linker, Adaptor, Custom, Reagent, Equipment), Application (PCR, DNA ... Oligonucleotide Synthesis Market by Product (Primer, Probe, Linker, Adaptor, Custom, Reagent, Equipment), Application (PCR, DNA ... Table 10 Market for Probes, By Region, 2015-2022 (USD Million). Table 11 North America: Market for Probes, By Country, 2015- ...
Identification of Nitrogen-Incorporating Bacteria in Petroleum-Contaminated Arctic Soils by Using [15N]DNA-Based Stable Isotope...
DNA buoyant density shifts during 15N-DNA stable isotope probing. Microbiol. Res. 162:328-334. ... Roche Diagnostics. 2009. Using multiplex identifier (MID) adaptors for the GS FLX titanium chemistry-extended MID set. ... DNA-based SIP (15N-SIP) is often complicated by a small degree of DNA separation between labeled and unlabeled DNA molecules ( ... DNA precipitation, and DNA quantitation were carried out as described previously (5, 6). In brief, equal amounts of DNA from ...
Patent US5679526 - Threshold ligand-receptor assay - Google Patents
Specific DNA probes in diagnostic microbiology. US4366241 *. 7 Aug 1980. 28 Dec 1982. Syva Company. Concentrating zone method ... Relia Diagnostic Systems, Llc. Lateral flow system and assay. US8221995. 27 Jun 2008. 17 Jul 2012. Seok-Won Lee. Methods and ... Diagnostic markers of acute coronary syndrome and methods of use thereof. US20030119064 *. 20 Aug 2002. 26 Jun 2003. Valkirs ... Relia Diagnostic Systems, Llc. Bidirectional lateral flow test strip and method. US7358055. 17 Aug 2005. 15 Apr 2008. Biosite, ...
Worldwide DNA Probes - Based Diagnostics Market - Global Industry Analysis, Size, Share, Growth, Trends, and Forecast, 2012 -...
DNA Probes-based diagnostics deals with the study to examine the chemical structure of DNA by undoing the presence of abnormal ... Also, U.S. and Europe together holds the majority of the global DNA Probes-based diagnostics market as about 80% to 90% of DNA ... The DNA Probes-based technology is extensively used in diagnostic and also in other fields such as instrumentation development ... Worldwide DNA Probes - Based Diagnostics Market - Global Industry Analysis, Size, Share, Growth, Trends, and Forecast, 2012 - ...
New Patent Granted to Nanomedical Diagnostics for Use of Graphene Biosensors with DNA Probes to Identify DNA Sequences for...
2017 Nanomedical Diagnostics, a life science company pioneering the use of label-free graphene biosensors in the healthcare ... New Patent Granted to Nanomedical Diagnostics for Use of Graphene Biosensors with DNA Probes to Identify DNA Sequences for ... New Patent Granted to Nanomedical Diagnostics for Use of Graphene Biosensors with DNA Probes to Identify DNA Sequences for ... The patent covers use of graphene biosensors with DNA probes to detect nucleic acids, and it builds on a previous comprehensive ...
DiagnosisMicrobiologyDetectionAssayMicroarraysSequencingRoche DiagnosticsHybridizationVitroSpecificityMicrobialAmplification2017-2021Random DNA probesOligonucleotideBacterialMonoclonalTransfusion diagnostics marketInfectious DiseasesBiochipsMarket is segmentedGeneticBacteriaForecastEstimates and projectionsGenomicPathogensBiosensorsBiochip technologiesComplementaryMolecular diagnosticImmunoassaysSensitivityNucleic acidsLaboratoryMethods2018Sequences1986TuberculosisGrowthSegmentPolymerase Chain ReSynthesis2022Specific DNATissueTaqManSputumTechnologyTherapeuticDetectGlobalClinical diagnosticsProducts
- The results indicate that the examination of respiratory tract secretions by the Gen-probe kit is a suitable screening test for the diagnosis of Legionnaires' disease. (nih.gov)
- If the DNA from a patient's sample binds with the complementary DNA in a disease probe, the diagnosis is positive for that disease. (phys.org)
- These probes have shown rapid and cost-effective microbial diagnosis, good sensitivity and specificity for some periodontal pathogens in cases of severe periodontitis. (thejcdp.com)
- The three types of bacteria can be detected rapidly with high sensitivity by using the DNA probe by general practitioners, and thus can help in the diagnosis process and the treatment. (thejcdp.com)
- The products under development include FISH Probes for the diagnosis of renal cancer, HPV-associated cancer test and Microarrays for the diagnosis of leukamia, ovarian cancer, cervical cancer, bladder cancer, prostate cancer amongst others. (sbwire.com)
- This comprehensive seven-country report will assist diagnostics industry executives, as well as companies planning to diversify into the dynamic and rapidly expanding microbiology market, in evaluating emerging opportunities and developing effective business strategies. (aarkstore.com)
- The spectroscopic measurements in the presence of well matched and mismatched DNA support the notion that our systems are interesting probes for mismatch detection and signaling. (caltech.edu)
- A commercial DNA probe kit (Gen-probe) for the detection of rRNA from legionellae was evaluated for its accuracy in diagnosing Legionnaires' disease in 167 patients with pneumonia. (nih.gov)
- OTCQX: RHHBY) today announced the launch of the new VENTANA HER2 Dual ISH DNA Probe Cocktail assay for the detection of the HER2 biomarker in breast and gastric cancer. (roche.com)
- 1989. Detection of rotavirus by hybridization with a nonradioactive synthetic DNA probe and comparison with commercial enzyme immunoassays and silver stained Polyacrylamide gels. (springer.com)
- Detection of enterotoxigenic Escherichia coli by dot blot hybridization with biotinylated DNA probes. (springer.com)
- The new patent enables the company's graphene biosensor technology to bring the benefits of lower costs, easier process, and higher throughput to the world of liquid biopsy and other DNA detection applications. (evonexus.org)
- Nanomedical Diagnostics ("Nanomed") is a life science company based in San Diego, CA. Nanomed has developed a breakthrough electrical assay based on proprietary Field Effect Biosensing (FEB) technology that delivers sensitive real-time direct detection of biomolecules. (evonexus.org)
- An extensive review of DNA probe and biochip technologies, test formats, detection methodologies, trends in testing automation and amplification methods. (mynewsdesk.com)
- Incorporating an immunomagnetic separation (IMS) step, followed by a secondary enrichment culturing step and DNA recovery with a QIAamp tissue kit (Qiagen), improved the detection threshold to ≥10 2 CFU/ml. (asm.org)
- With an optimal DNA extraction method, LiPA allows rapid detection of M. tuberculosis complex DNA and RMP resistance directly from sputum specimens. (asm.org)
- These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. (mdpi.com)
- Our latest RUO kit, the Luna ® SARS-CoV-2 RT-qPCR Multiplex Assay Kit , enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. (neb.com)
- Yahfoufi ZA, Hadchiti W, Berberi A. Deoxyribonucleic Acid Probes Analyses for the Detection of Periodontal Pathogens. (thejcdp.com)
- Deoxyribonucleic acid probes can detect bacteria at very low level about 10 3 which is below the detection level of culture methods. (thejcdp.com)
- DNA probe detection of periodontal pathogens. (thejcdp.com)
- Many diagnostic assays have been developed which are based on either immunochemical detection of proteins, e.g. (google.com)
- or the detection of nucleic acids, either RNA or DNA, present in or produced by target cells, e.g. (google.com)
- A chemical method for fast and sensitive detection of DNA synthesis in vivo. (abcam.com)
- Labelling and detection of probes. (muni.cz)
- The diagnostic performance of the DNA probe assay in this study was superior to that of the DFA test. (nih.gov)
- The VENTANA HER2 Dual ISH DNA Probe Cocktail assay 3 aids in identifying breast and gastric cancer patients eligible for the targeted Roche drug Herceptin (trastuzumab), providing fast results with widely available laboratory instruments. (roche.com)
- The VENTANA HER2 Dual ISH DNA Probe Cocktail assay is designed to be completed within the same day, providing clinicians the ability to get results back quicker than the most common methods of confirmatory testing for HER2. (roche.com)
- The new VENTANA HER2 Dual ISH assay advances Roche's commitment to personalized healthcare by delivering critical information on treatment options for breast and gastric cancer patients faster," said Michael Heuer, CEO Roche Diagnostics. (roche.com)
- For more information about the assay, please visit the Roche Tissue Diagnostics breast cancer IHC/ISH portfolio page or the Anatomic Pathology site. (roche.com)
- With the new VENTANA HER2 Dual ISH DNA Probe Cocktail assay, Roche provides an improved brightfield assay that is fully automated on the BenchMark IHC/ISH instruments. (roche.com)
- The patent covers use of graphene biosensors with DNA probes to detect nucleic acids, and it builds on a previous comprehensive patent for monitoring biomolecular interactions on which the company's flagship product, the Agile R100 personal assay system, is based. (evonexus.org)
- Hybridization assay employing labeled probe and anti-hybrid. (freepatentsonline.com)
- In this report, we describe the development and evaluation of the sensitivity and specificity of a PCR-based 5′ nuclease assay for presumptively detecting E. coli O157:H7 DNA. (asm.org)
- The APTIMA Trichomonas vaginalis Assay (Gen-Probe, San Diego, Calif) uses nucleic acid hybridization technology to detect the presence of T vaginalis . (medscape.com)
- The BD ProbeTec TV Qx Amplified DNA Assay is a DNA-amplification test developed for the BD Viper system, a proprietary automated platform capable of testing for T vaginalis , Chlamydia trachomatis , N gonorrhoeae , HSV-1, and HSV-2. (medscape.com)
- The life science business unit specializes in genomics, and involves the development of oligonucleotides, DNA polymerases, Real-time qPCR Probes, assay services, and proteomics. (wikipedia.org)
- Roche NimbleGen is a leading innovator, manufacturer, and supplier of a proprietary suite of DNA microarrays, consumables, instruments and services. (pressebox.com)
- The improved performance is made possible by Roche NimbleGen's proprietary Maskless Array Synthesis (MAS) technology, which uses digital light processing and rapid, high-yield photochemistry to synthesize long oligo, high-density DNA microarrays with extreme flexibility. (pressebox.com)
- Compressive sensing DNA microarrays. (nih.gov)
- The company offers products such as CGH Microarrays, Next-Generation Sequencing and DNA-FISH Probes. (mynewsdesk.com)
- Represented biotechnology company in patent litigation relating to methods for manufacturing DNA microarrays. (cov.com)
- More recent genomic identification methods that use polymerase chain reaction, or PCR, and genomic sequencing can be faster but require expensive equipment and training as well as a specific DNA probe for each pathogen to be tested. (phys.org)
- Sanger DNA Sequencing g. (aarkstore.com)
- Different methods of sequencing of nucleic acids and analysis of DNA sequences. (muni.cz)
- Recombinant human and murine IFN-γ were purchased from Biosource Europe and Roche Diagnostics, respectively. (rupress.org)
- Total RNA was extracted using a MagnaPure LC RNA Isolation Kit (Roche Diagnostics). (rupress.org)
- RT- and real-time PCR reactions were then performed using LightCycler-RNA Master Hybridization Probes (one-step procedure) on a Lightcycler ® apparatus (Roche Diagnostics). (rupress.org)
- Roche NimbleGen, a company of Roche announced today the launch of comparative genomic hybridization (CGH) arrays and services for the high-resolution mapping of genomic copy-number differences based on the company's new 2.1 million-probe HD2 platform. (pressebox.com)
- These new multiplex arrays allow the parallel hybridization of four samples to four separate 72,000 long oligo probe arrays on a single slide, providing a cost-effective, increased throughput option for CGH analysis. (pressebox.com)
- In situ hybridization with biotinylated DNA probes: a rapid diagnostic test for adenovirus upper respiratory infections. (semanticscholar.org)
- Adenovirus DNA was detected in cells from nasopharyngeal secretions of children with acute respiratory infections by in situ hybridization with biotinylated probes. (semanticscholar.org)
- Hybridization-capture polymerase chain reaction (HC-PCR), a nucleic acid sequence capture technique, was evaluated on faecal samples pooled from 50 sheep and individual faecal samples as a rapid diagnostic test for Mycobacterium avium subsp. (nih.gov)
- The lack of sensitivity of HC-PCR was neither due to location nor length of capture probe on IS 900 nor deterioration of the probe but was associated with inefficiencies in liquid phase hybridization and solid phase magnetic bead capture. (nih.gov)
- more particularly, the invention provides compositions for detecting activated T-cells by nucleic acid hybridization probes and/or immunochemical assays. (google.com)
- The molecular diagnostics market is unquestionably the most rapidly growing segment of the in vitro diagnostics industry. (bio-medicine.org)
- Roche is the world leader in in-vitro diagnostics and drugs for cancer and transplantation, and is a market leader in virology. (pressebox.com)
- The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally. (google.com)
- 2. Outlook for the In Vitro Diagnostics Market a. (reportsnreports.com)
- Manufacturers of in vitro diagnostics are increasingly calling on suppliers to assist them in meeting growing demands of industry regulators in both the US and Europe. (sigmaaldrich.com)
- Importantly, the regulations also apply to in vitro diagnostics, including the chemical and biological components they contain, so that any raw materials destined for a diagnostic or to be used in the manufacture of a diagnostic kit must meet specific requirements. (sigmaaldrich.com)
- 2007: In order to fully meet the Oligonucleotide in vitro diagnostics stringent manufacturing requirements, Eurogentec expanded its current GMP facility in Liège. (wikipedia.org)
- 2008: Eurogentec received ISO 13485 Certification for the production and sales of In Vitro Diagnostics (IVD) oligonucleotides in Liège. (wikipedia.org)
- Eurogentec has developed three interrelated business units, Life Science Research Products and Services, In Vitro Diagnostics Manufacturing Solutions, and GMP BioManufacturing. (wikipedia.org)
- The in vitro diagnostics business unit provides technical and project support for contract manufacturing of good manufacturing practice (GMP) Oligonucleotides and Taq DNA polymerases for use in Molecular Diagnostic applications. (wikipedia.org)
- Previous studies have shown that rhodium metalloinsertors bind mismatches in double-strained DNA with high specificity. (caltech.edu)
- Antibodies are highly sought after for diagnostic applications owing to its specificity and affinity towards a target antigen. (intechopen.com)
- Results using either method must be interpreted with considerable caution, because poor sensitivity and specificity of these diagnostic tests has been demonstrated in several studies. (medscape.com)
- Rice's "universal microbial diagnostic," or UMD, uses pieces of randomly assembled DNA and mathematical techniques that were originally pioneered for signal processors inside digital phones and cameras. (phys.org)
- With universal microbial diagnostic, we won't need new probes or to change any other parts of the sensing hardware," he said. (phys.org)
- Recently, Deoxyribonucleic acid (DNA)-based techniques have been introduced to detect human microbial pathogens in periodontal diseases. (thejcdp.com)
- The Global DNA Probes-Based Diagnostic Market Research Report Forecast 2017-2021 is a valuable source of insightful data for business strategists. (researchnreports.com)
Random DNA probes3
- Rice University researchers (clockwise from left) Richard Baraniuk, Amirali Aghazadeh and Rebekah Drezek have invented a technology that could potentially identify hundreds of bacterial pathogens simply, quickly and at low cost using a single set of random DNA probes. (phys.org)
- Rice University scientists have invented a technology that could potentially identify hundreds of bacterial pathogens simply, quickly and at low cost using a single set of random DNA probes. (phys.org)
- In a paper online this week in Science Advances, Rice's research team used lab tests to verify that UMD could identify 11 known strains of bacteria using the same five random DNA probes. (phys.org)
- Specific oligonucleotide probes were developed to identify medically important fungi that display yeast-like morphology in vivo. (asm.org)
- Specific oligonucleotide probes to identify these fungi, as well as a probe to detect all dimorphic, systemic pathogens, were developed. (asm.org)
- κB#1wt probe was generated by end labeling the double stranded oligonucleotide 5′-AGAGTCCCGGGAAAGTCCTGCCGCGCC-3′ (corresponding to nt −68 to −42), and electrophoretic mobility shift assays (EMSAs) were performed as described previously ( 9 ). (rupress.org)
- Eurogentec operates two manufacturing facilities in Belgium that provide custom biologics and oligonucleotide-based components for diagnostic and therapeutic/prophylactic applications. (wikipedia.org)
- Two years after EGT NA also received ISO 13485 certification for the Oligonucleotide diagnostics manufacturing. (wikipedia.org)
- Because the probes are not specific to a particular disease, the technology provides a genomic-based bacterial identification system that does not require a one-to-one ratio of DNA probes to pathogenic species. (phys.org)
- The lack of rapid bacterial diagnostics can promote antibiotic resistance. (phys.org)
- Several families of Proteobacteria and Actinobacteria that are directly involved in petroleum degradation incorporated the added nitrogen in contaminated soils, but it was the DNA of Sphingomonadaceae that was most enriched in 15 N. Bacterial growth in uncontaminated soils was not stimulated by nutrient amendment. (asm.org)
- As a global leader in breast cancer diagnostics, Roche provides a comprehensive menu of both diagnostic and predictive assays, including the VENTANA HER2/neu (4B5) Rabbit Monoclonal Primary Antibody that is indicated as an aid in the assessment of breast cancer patients for whom Herceptin treatment is considered. (roche.com)
- Monoclonal antibodies to pneumocystis proteins and sensitive DNA probes have been developed but have yet to reach the bedside. (bmj.com)
Transfusion diagnostics market2
- This comprehensive seven-country report is designed to help current suppliers and potential market entrants identify and evaluate emerging opportunities in the global transfusion diagnostics market during the next ten years, and assist industry executives in developing effective business, new product development and marketing strategies. (bio-medicine.org)
- Assessment of current and emerging technologies, and their potential applications for the transfusion diagnostics market. (bio-medicine.org)
- Infectious Diseases represents the key end-use market for DNA probes-based diagnostics worldwide. (prweb.com)
- The major application area for DNA Probes-based diagnostics market includes Infectious Diseases, genetic predispositions, Molecular HLA (Human Leukocyte Antigen) typing, Forensic, Cancver testing, and others. (blogspot.com)
- The infectious diseases segment exhibits the largest application segment for DNA Probes-based diagnostics market. (blogspot.com)
- Reportlinker Adds Molecular Diagnostics Market: DNA Probes and Biochips -- New Product Development Opportunities and Business Expansion Strategies for Instrument and Reagent. (bio-medicine.org)
Market is segmented2
- DNA Probe-based Diagnostic market is segmented by region (country), players, by Type, and by Application. (reportsnreports.com)
- Based on product, the phytopathological disease diagnostics market is segmented into kits, lateral flow devices, DNA/RNA probes, accessories, other consumables, and software and cloud databases. (pharmiweb.com)
- The U.S. holds a major share of the global DNA Probes-based diagnostics market as transformational technologies enabling the complex genetic tests to shift from research only laboratories to medical and clinical laboratories. (blogspot.com)
- The main goal of LightDyNAmics is to achieve a complete understanding of the ultrafast dynamical processes at the molecular scale induced by UV light absorption in DNA, and to unveil the mechanisms leading to photodamage of the genetic code. (europa.eu)
- This panel of DNA probes is designed to provide diagnostic genetic information for patients with myeloproliferative disorders. (legacyhealth.org)
- It offers cancer diagnostics and treatments tailoring the specific genetic profile of the individual. (sbwire.com)
- Genetic Variability in Nuclear DNA in Field Populations of Stagonospora nodorum . (apsnet.org)
- The course is destined for the students with interest in advanced molecular genetic and diagnostic methods which are used in analysis of microorganisms. (muni.cz)
- The new study includes several computer simulations, including one that shows how a random selection of five probes can identify 40 different strains of bacteria, and another that demonstrates how the system can accurately differentiate between 24 different species of Staphylococcus. (phys.org)
- Various procedures and probes are available for the analysis of bacteria isolated from shell disease lesions (Chistoserdov et al. (gc.ca)
- The innuSPEED Bacteria/Fungi DNA Kit has been specially devel-oped for isolating genomic DNA from gram+ bacteria, fungal spores and yeasts. (environmental-expert.com)
- Deoxyribonucleic acid probes can detect bacteria at a very low level if we compared with the culture methods. (thejcdp.com)
- The global market for DNA Probe-Based Diagnostics is forecast to reach US$30 billion by the year 2017, driven by the rapidly expanding molecular diagnostics market. (prweb.com)
- New York, NY -- ( SBWIRE ) -- 04/16/2018 -- The latest market intelligence study on DNA Probe-based Diagnostic market relies on statistics derived from the application of both primary and secondary research to present insights pertaining to the operational model, opportunities and competitive landscape of DNA Probe-based Diagnostic market for the forecast period, 2018 - 2025. (sbwire.com)
- The research also segments the DNA Probe-based Diagnostic market on the basis of the end-user, product type, application and demography for the forecast period 2018 - 2025. (sbwire.com)
- For more clarity on the real potential of the DNA Probe-based Diagnostic market for the forecast period of 2018 - 2025 the study provides vital intelligence on the major opportunities, threats and challenges posed by the industry. (sbwire.com)
- What is estimated growth rate and market size of the DNA Probe-based Diagnostic industry for the forecast period 2018 - 2025? (sbwire.com)
- Which market trends from the yesteryears and the future are likely to keep the prospect of the DNA Probe-based Diagnostic market high for the forecast period 2018 - 2025? (sbwire.com)
- This chapter explains the key trends that are expected to influence the growth of the phytopathological disease diagnostics market during the forecast period. (pharmiweb.com)
- This section explains the global market value analysis and forecast for the phytopathological disease diagnostics during the forecast period. (pharmiweb.com)
Estimates and projections1
- The report provides annual sales estimates and projections for DNA Probe-Based Diagnostics market for the years 2009 through 2017 for the following geographic markets - US, Canada, Japan, Europe, Asia-Pacific and Latin America. (prweb.com)
- Roche NimbleGen uniquely produces high-density arrays of long oligo probes that provide greater information content and higher data quality necessary for studying the full diversity of genomic and epigenomic variation. (pressebox.com)
- Genomic DNA probes were made for five members of the Anopheles punctulatus complex of mosquitoes found in Papua New Guinea. (ajtmh.org)
- farauti No. 6, by differentially screening total genomic DNA libraries of individual species and sibling species with homologous DNA against heterologous DNA labeled with 32 P. Probes ranged from 273 to 630 bp. (ajtmh.org)
- The innuSPEED Tissue DNA Kit has been specially designed for using homogenizers in the process of isolating genomic DNA from an exceptionally wide variety of starting materials. (environmental-expert.com)
- In conclusion, probes developed to yeast-like pathogens were found to be highly specific and should prove to be useful in differentiating these organisms in the clinical setting. (asm.org)
- This study shows a high frequency of putative periodontal pathogens by using DNA probe technology, which is semi-quantitative in this study. (thejcdp.com)
- Nanomedical Diagnostics , a life science company pioneering the use of label-free graphene biosensors in the healthcare industry, announces the grant of U.S. Patent No. 9,765,395 by the United States Patent and Trademark Office. (evonexus.org)
- The extraordinary sensitivity of graphene biosensors also creates the opportunity to detect very low concentrations of circulating tumor DNA, possibly improving patient outcomes. (evonexus.org)
- As the world's only mass-manufacturer of graphene biosensors, Nanomed's mission is to transform the healthcare industry with innovative new products that enable cutting-edge life science research, drug discovery applications, and diagnostic and health monitoring platforms. (evonexus.org)
- In order to successfully capitalize on the opportunities presented by the molecular diagnostics market, many companies are already exploiting new DNA probe and biochip technologies as corporate strategic assets, managed in support of business and marketing strategies. (bio-medicine.org)
- The probes are snippets of complementary DNA from a known disease. (phys.org)
- Rather than identifying a target strain based on a 100 percent match with a specific probe, Rice's system tests how well the target DNA binds with several different random segments of complementary DNA. (phys.org)
- This will be achieved by performing independent, yet interrelated and complementary research projects focussed on photoactivated dynamics of DNA, and by developing a variety of new spectroscopic and computational methods. (europa.eu)
- The latter assays use nucleic acid probes, usually fluorescently labeled or radioactively labeled DNAs or RNAs, which can be preferentially hybridized to complementary target nucleic acids in appropriately prepared samples or tissues. (google.com)
- Antibodies have been utilized successfully in various diagnostic platforms ranging from standard immunoassays to lateral-flow assays, nanoparticles, microfluidics, DNA‐integrated assays and others. (intechopen.com)
- this obviates the need for bronchoscopy but the diagnostic sensitivity is lower. (bmj.com)
- Follow us on LinkedIn - In the medical diagnostics laboratories of the 21st century, the ability to detect nucleic acids and proteins reign supreme. (prweb.com)
- Dr. Stole was a post-doctoral fellow at Johns Hopkins University (1992-1996) where he conducted research on enzymes involved in reactions with and repair of DNA and other nucleic acids. (cov.com)
- If a laboratory today wants to test for 200 known pathogenic species, they need 200 different tests, each with its own specific DNA probe that was designed specifically to bind with DNA from a particular pathogen," said study co-author Richard Baraniuk, the lead scientist on the new study. (phys.org)
- The report is based on combination of primary and secondary information sources, including interviews with blood bankers, laboratory directors, and executives of leading diagnostics companies in the U.S., five major European countries, and Japan. (bio-medicine.org)
- Major issues pertaining to the Japanese molecular diagnostics laboratory practice, as well as key economic, regulatory, demographic, social and technological trends with significant market impact during the next five years. (mynewsdesk.com)
- Azar MM, Hage CA. Laboratory diagnostics for histoplasmosis. (cdc.gov)
- Liquid biopsy has shown tremendous promise for cancer diagnostics, but current methods have cost and process challenges to overcome," says Nanomedical Diagnostics CEO, Ross Bundy. (evonexus.org)
- Required Textbook: Molecular Diagnostics: Fundamentals, Methods and Clinical Applications, 2nd edition. (austincc.edu)
- Moreover, the true burden of fungal disease is most certainly underestimated because of the insensitivity of present diagnostic methods ( 8 , 18 , 25 ). (asm.org)
- The invention relates to nucleic acid multiplexes, and more particularly to methods wherein quadruplexes are formed by specific binding between double-stranded nucleic acid probes and double-stranded nucleic acid target sequences. (google.com)
- Dur- ing the first week, the biology, molecular biology and pathophysiology of myco- plasmas, as well as all the main diagnostic methods were covered, including both conventional and the newer technologies. (springer.com)
- Dr. Stole's litigation and counseling experience has focused on matters involving pharmaceuticals, chemicals, chemical processes, and biotechnology such as genetically-engineered enzymes and DNA-based diagnostic methods. (cov.com)
- A recent market study published by Future Market Insights (FMI) on the phytopathological disease diagnostics including global industry analysis of 2014-2018 and opportunity assessment for 2019-2029, delivers a comprehensive assessment of the most important market dynamics. (pharmiweb.com)
- Testing DNA samples for particular nucleotide sequences. (freepatentsonline.com)
- 1986. Probing of Campylobacter jejuni with DNA coding for Escherichia coli heat-labile enterotoxin. (springer.com)
- To assess the utility of this test for detecting Mycobacterium tuberculosis complex DNA and rifampin (RMP) resistance, 420 sputum samples comprising specimens from untreated ( n = 160) and previously treated ( n = 260) patients from 11 countries in Asia, Africa, Europe, and Latin America were tested. (asm.org)
- The LiPA test was positive for M. tuberculosis complex DNA in 389 (92.9%) specimens, including 92.0% (286 of 311) ZN-positive and 94.5% (103 of 109) ZN-negative specimens. (asm.org)
- LiPA detected M. tuberculosis complex DNA in 92.4% (110 of 119) of the culture-positive and 100.0% (41 of 41) of the culture-negative specimens from untreated patients. (asm.org)
- Growth in the marketplace is also expected to stem from affluent population and widening health insurance as well as medical reimbursements, which are making expensive cutting edge diagnostic tools such as DNA-based diagnostics increasingly affordable. (prweb.com)
- In the US, the rapid growth of DNA-based diagnostics is leading to the development of new tests for the treatment of non-contagious diseases, such as diseases related to aging or chronic diseases. (prweb.com)
- The research report titled "DNA Probes-Based Diagnostics: A Global Strategic Business Report" announced by Global Industry Analysts Inc., provides a comprehensive review of the DNA probe-based diagnostics markets, current market trends, key growth drivers, recent product introductions, recent industry activity, and profiles of major/niche global as well as regional market participants. (prweb.com)
- In addition, the research on the DNA Probe-based Diagnostic market concentrates on extracting valuable data on swelling investment pockets, significant growth opportunities and major market vendors to help understand business owners what their competitors are doing best to stay ahead in the competition. (sbwire.com)
- It provides the DNA Probes-Based Diagnostic industry overview with growth analysis and historical & futuristic cost, revenue, demand and supply data (as applicable). (researchnreports.com)
- After conducting thorough research on the historical as well as current growth parameters of the phytopathological disease diagnostics market, growth prospects are obtained with maximum precision. (pharmiweb.com)
- com/research/rgm4tc/20152019_global) has announced the addition of the "2015-2019 Global Pseudomonas Diagnostic Testing Market: Emerging Opportunities and Growth Strategies" report to their offering. (thefreedictionary.com)
Polymerase Chain Re1
- Progress towards a rapid polymerase chain reaction diagnostic test for the identification of Mycobacterium avium subsp. (nih.gov)
- A flow cell which can be used in the synthesis of DNA probes on an active surface of the substrate includes a base having a central window opening and a registration surface against which the substrate may be mounted. (google.com)
- Alternative to BrdU (5-Bromo-2'-deoxyuridine) ( ab142567 ) for measuring de novo DNA synthesis during S-phase. (abcam.com)
- Used for probing short-term DNA synthesis in tissue culture and living organisms where prolonged cell survival is not required. (abcam.com)
- Represented biotechnology company in patent litigation involving synthesis and use of DNA libraries. (cov.com)
- 1999: Eurogentec acquired Oswel Research Product Ltd. a biotechnology company based in Southampton, Great Britain and specialized in the synthesis of chemically modified and complex oligonucleotides and their analogues (DNA, RNA, PNA). (wikipedia.org)
- DNA Probe-based Diagnostic Market 2017-2022, has been prepared based on an in-depth market analysis with inputs from industry experts. (openpr.com)
- Specific DNA probes were developed for An. (ajtmh.org)
- The innuPREP Virus DNA/RNA allows researchers to purify viral ssDNA or dsDNA and ssRNA simultaneously from serum, plasma or other cell-free bodily fluids, from tissue, paraffin or swab samples, or from cell cultures. (environmental-expert.com)
- Molecular diagnostics, such as PCR on tissue specimens, are increasingly available to support microscopic findings, although the performance of these tests may vary. (cdc.gov)
- TaqMan probes, hybridisation probes, molecular beacons. (muni.cz)
- DNA was extracted from sputum samples by using a modification of the Boom's method, while the rpoB core region was amplified by nested PCR. (asm.org)
- Integrating new technology planning with business and corporate strategies will be one of the most challenging tasks for diagnostics companies during the next ten years. (bio-medicine.org)
- The DNA Probes-based technology is extensively used in diagnostic and also in other fields such as instrumentation development, molecular biology, surface chemistry, microfabrication, biological sample preparation and data handling. (blogspot.com)
- This chapter provides details about the phytopathological disease diagnostics market based on test, and has been classified into molecular tests, serological tests, and test using electronic technology. (pharmiweb.com)
- Siemens Healthineers applied compressed sensing technology to help solve a clinical problem: how to reduce long scan times while maintaining high diagnostic quality . (phys.org)
- Previously, low diagnostic quality prevented these patient subsets from realizing any benefit from cardiac MRI, but the technology enables the entire cardiac cycle to be recorded in real time with only one breath-hold. (phys.org)
- The smart Blood DNA Midi prep (a) is based on the unique SmartExtraction technology and allows an easy automation of DNA extraction from up to 3 ml of whole blood. (environmental-expert.com)
- Technology of recombinant DNA and their importance for study of microorganisms. (muni.cz)
- The multiplex structure and the process of forming it have diagnostic, therapeutic, prophylactic and nanoengineering applications. (google.com)
- Light interaction with biomaterials is the driving mechanism of fundamental biological processes, from photosynthesis to DNA photodamage, and is a powerful tool in biomedicine for analytical, diagnostic and therapeutic purposes. (europa.eu)
- Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. (abcam.com)
- The future envisages a DNA probe, which offers the ability to detect complex interrelationships between multiple gene expression and environmental factors, at the molecular level. (prweb.com)
- Diagnostic tests are also taking advantage of the small sizes, using probes of unusually shaped DNA that can detect cancer with remarkable accuracy. (scientificamerican.com)
- Burgeoning Molecular Diagnostics Market Propels the Global DNA Probes-Based Diagnostics Market, According to New Report by Global Industry Analysts, Inc. (prweb.com)
- GIA announces the release of a comprehensive global report on DNA Probe-Based Diagnostics markets. (prweb.com)
- The report on "Global DNA Probe-based Diagnostic Market" is a professional report which provides thorough knowledge along with complete information pertaining to the DNA Probe-based Diagnostic industry a propos classifications, definitions, applications , industry chain summary, industry policies in addition to plans, product specifications, manufacturing processes, cost structures, etc. (openpr.com)
- The report cloaks the market analysis and projection of "DNA Probe-based Diagnostic Market" on a regional as well as global level. (openpr.com)
- The Global DNA Probe-based Diagnostic Market Research Report renders deep perception of the key regional market status of the DNA Probe-based Diagnostic Industry on a global level that primarily aims the core regions which comprises of continents like Europe, North America, and Asia and the key countries such as United States, Germany, China and Japan. (openpr.com)
- It is a methodical research depending on the market and examines the competitive framework of the global DNA Probe-based Diagnostic Market industry. (openpr.com)
- There are 14 Chapters to deeply display the global DNA Probe-based Diagnostic Profiler market. (sbwire.com)
- Players, stakeholders, and other participants in the global DNA Probe-based Diagnostic market will be able to gain the upper hand as they use the report as a powerful resource. (reportsnreports.com)
- Roche is a global pioneer in pharmaceuticals and diagnostics focused on advancing science to improve people's lives. (roche.com)
- Also, U.S. and Europe together holds the majority of the global DNA Probes-based diagnostics market as about 80% to 90% of DNA tests are performed in the developed regions such as North America and Europe. (blogspot.com)
- Global Phytopathological Disease Diagnostics Market on a. (pharmiweb.com)
- The global phytopathological disease diagnostics is segmented in detail to cover every aspect of the market and present a complete market intelligence approach to the reader. (pharmiweb.com)
- Dipsticks are one of the cheapest paper diagnostic products available in the global IVD market (parent market). (reportbuyer.com)
- Pharmaceutical and biotechnology companies are one of the major market contributors to the global CTC diagnostics market. (reportbuyer.com)
- Diba Industries creates and delivers precision fluid handling systems to global OEMs in diagnostics and medical devices. (jspargo.com)
- High Heska Heska sells advanced veterinary diagnostic and specialty products. (reportbuyer.com)
- Cancer Genetics Inc (CGI) is a pharmaceutical company that provides diagnostics products and services in oncology areas. (mynewsdesk.com)
- The diagnostic assays and products offered by the company are designed to increase treatment efficacy and reduce healthcare costs of the hematological, urogenital and HPV-associated cancers. (sbwire.com)