A nucleoside consisting of the base guanine and the sugar deoxyribose.
Guanine nucleotides which contain deoxyribose as the sugar moiety.
A purine or pyrimidine base bonded to DEOXYRIBOSE.
Adenosine molecules which can be substituted in any position, but are lacking one hydroxyl group in the ribose part of the molecule.
A purine nucleoside that has guanine linked by its N9 nitrogen to the C1 carbon of ribose. It is a component of ribonucleic acid and its nucleotides play important roles in metabolism. (From Dorland, 28th ed)
The products of chemical reactions that result in the addition of extraneous chemical groups to DNA.
An enzyme that catalyzes the reaction between a purine nucleoside and orthophosphate to form a free purine plus ribose-5-phosphate. EC 2.4.2.1.
Purines with a RIBOSE attached that can be phosphorylated to PURINE NUCLEOTIDES.
A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.
An enzyme that catalyzes reversibly the phosphorylation of deoxycytidine with the formation of a nucleoside diphosphate and deoxycytidine monophosphate. Cytosine arabinoside can also act as an acceptor. All natural nucleoside triphosphates, except deoxycytidine triphosphate, can act as donors. The enzyme is induced by some viruses, particularly the herpes simplex virus (HERPESVIRUS HOMINIS). EC 2.7.1.74.
A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A class of chemicals that contain an anthracene ring with a naphthalene ring attached to it.
Organic compounds containing a carbonyl group in the form -CHO.
Nucleosides containing arabinose as their sugar moiety.
Enzymes of the transferase class that catalyze the transfer of a pentose group from one compound to another.
Adenine nucleotides which contain deoxyribose as the sugar moiety.
Used in the form of its salts as a dye and as an intermediate in manufacture of Acid Yellow, diazo dyes, and indulines.
Purine or pyrimidine bases attached to a ribose or deoxyribose. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
7,8,8a,9a-Tetrahydrobenzo(10,11)chryseno (3,4-b)oxirene-7,8-diol. A benzopyrene derivative with carcinogenic and mutagenic activity.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A potent mutagen and carcinogen. It is a public health concern because of its possible effects on industrial workers, as an environmental pollutant, an as a component of tobacco smoke.
A group of compounds that are derivatives of methoxybenzene and contain the general formula R-C7H7O.
Common name for one of five species of small PARROTS, containing long tails.
Drugs that inhibit ADENOSINE DEAMINASE activity.
A member of the BENZODIOXOLES that is a constituent of several VOLATILE OILS, notably SASSAFRAS oil. It is a precursor in the synthesis of the insecticide PIPERONYL BUTOXIDE and the drug N-methyl-3,4-methylenedioxyamphetamine (MDMA).
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
A species of gram-positive, rod-shaped bacteria isolated from the intestinal tract of humans and animals, the human mouth, and vagina. This organism produces the fermented product, acidophilus milk.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.
A species of toxic plants of the Compositae. The poisonous compounds are alkaloids which cause cattle diseases, neoplasms, and liver damage and are used to produce cancers in experimental animals.
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
Purines attached to a RIBOSE and a phosphate that can polymerize to form DNA and RNA.
The monomeric units from which DNA or RNA polymers are constructed. They consist of a purine or pyrimidine base, a pentose sugar, and a phosphate group. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A purine nucleoside that has hypoxanthine linked by the N9 nitrogen to the C1 carbon of ribose. It is an intermediate in the degradation of purines and purine nucleosides to uric acid and in pathways of purine salvage. It also occurs in the anticodon of certain transfer RNA molecules. (Dorland, 28th ed)
The rate dynamics in chemical or physical systems.
Benzopyrenes saturated in any two adjacent positions and substituted with two hydroxyl groups in any position. The majority of these compounds have carcinogenic or mutagenic activity.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
An enzyme that catalyzes the hydrolysis of ADENOSINE to INOSINE with the elimination of AMMONIA.
A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).
Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.
Nucleotides in which the purine or pyrimidine base is combined with ribose. (Dorland, 28th ed)
Syndromes in which there is a deficiency or defect in the mechanisms of immunity, either cellular or humoral.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Nucleotides containing arabinose as their sugar moiety.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
Organic compounds that include a cyclic ether with three ring atoms in their structure. They are commonly used as precursors for POLYMERS such as EPOXY RESINS.
Diseases caused by abnormal function of the MITOCHONDRIA. They may be caused by mutations, acquired or inherited, in mitochondrial DNA or in nuclear genes that code for mitochondrial components. They may also be the result of acquired mitochondria dysfunction due to adverse effects of drugs, infections, or other environmental causes.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
Nucleosides in which the purine or pyrimidine base is combined with ribose. (Dorland, 28th ed)
A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.
Unstable isotopes of phosphorus that decay or disintegrate emitting radiation. P atoms with atomic weights 28-34 except 31 are radioactive phosphorus isotopes.
DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.
A glycoprotein enzyme present in various organs and in many cells. The enzyme catalyzes the hydrolysis of a 5'-ribonucleotide to a ribonucleoside and orthophosphate in the presence of water. It is cation-dependent and exists in a membrane-bound and soluble form. EC 3.1.3.5.
A purine base and a fundamental unit of ADENINE NUCLEOTIDES.
The covalent bonding of an alkyl group to an organic compound. It can occur by a simple addition reaction or by substitution of another functional group.
Double-stranded DNA of MITOCHONDRIA. In eukaryotes, the mitochondrial GENOME is circular and codes for ribosomal RNAs, transfer RNAs, and about 10 proteins.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
The process by which a DNA molecule is duplicated.
Phosphate esters of THYMIDINE in N-glycosidic linkage with ribose or deoxyribose, as occurs in nucleic acids. (From Dorland, 28th ed, p1154)
The dialdehyde of malonic acid.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
An enzyme that catalyzes the conversion of 5-phosphoribosyl-1-pyrophosphate and hypoxanthine, guanine, or 6-mercaptopurine to the corresponding 5'-mononucleotides and pyrophosphate. The enzyme is important in purine biosynthesis as well as central nervous system functions. Complete lack of enzyme activity is associated with the LESCH-NYHAN SYNDROME, while partial deficiency results in overproduction of uric acid. EC 2.4.2.8.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC 2.7.1.21.
Established cell cultures that have the potential to propagate indefinitely.
Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
Organic compounds that contain phosphorus as an integral part of the molecule. Included under this heading is broad array of synthetic compounds that are used as PESTICIDES and DRUGS.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
A DNA repair enzyme that catalyzes DNA synthesis during base excision DNA repair. EC 2.7.7.7.
Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.

Exposure to indoor background radiation and urinary concentrations of 8-hydroxydeoxyguanosine, a marker of oxidative DNA damage. (1/1399)

We investigated whether exposure to indoor [gamma]-radiation and radon might be associated with enough free radical formation to increase urinary concentrations of 8-hydroxydeoxyguanosine (8-OHdG), a sensitive marker of DNA damage, due to a hydroxyl radical attack at the C8 of guanine. Indoor radon and [gamma]-radiation levels were measured in 32 dwellings for 6 months by solid-state nuclear track detectors and thermoluminescent dosimeters, respectively. Urine samples for 8-OHdG determinations were obtained from 63 healthy adult subjects living in the measured dwellings. An overall tendency toward increasing levels of 8-OHdG with increasing levels of radon and [gamma]-radiation was seen in the females, presumably due to their estimated longer occupancy in the dwellings measured. Different models were considered for females, with the steepest slopes obtained for [gamma]-radiation with a coefficient of 0.500 (log nmol/l of 8-OHdG for each unit increase of [gamma]-radiation on a log scale) (p<0.01), and increasing to 0.632 (p = 0.035), but with larger variance, when radon was included in the model. In conclusion, there seems to be an effect of indoor radioactivity on the urinary excretion of 8-OHdG for females, who are estimated to have a higher occupancy in the dwellings measured than for males, for whom occupational and other agents may also influence 8-OHdG excretion. ree radicals; [gamma]-radiation; radon.  (+info)

RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease. (2/1399)

In this study we used an in situ approach to identify the oxidized nucleosides 8-hydroxydeoxyguanosine (8OHdG) and 8-hydroxyguanosine (8OHG), markers of oxidative damage to DNA and RNA, respectively, in cases of Alzheimer's disease (AD). The goal was to determine whether nuclear and mitochondrial DNA as well as RNA is damaged in AD. Immunoreactivity with monoclonal antibodies 1F7 or 15A3 recognizing both 8OHdG and 8OHG was prominent in the cytoplasm and to a lesser extent in the nucleolus and nuclear envelope in neurons within the hippocampus, subiculum, and entorhinal cortex as well as frontal, temporal, and occipital neocortex in cases of AD, whereas similar structures were immunolabeled only faintly in controls. Relative density measurement showed that there was a significant increase (p < 0.0001) in 8OHdG and 8OHG immunoreactivity with 1F7 in cases of AD (n = 22) as compared with senile (n = 13), presenile (n = 10), or young controls (n = 4). Surprisingly, the oxidized nucleoside was associated predominantly with RNA because immunoreaction was diminished greatly by preincubation in RNase but only slightly by DNase. This is the first evidence of increased RNA oxidation restricted to vulnerable neurons in AD. The subcellular localization of damaged RNA showing cytoplasmic predominance is consistent with the hypothesis that mitochondria may be a major source of reactive oxygen species that cause oxidative damage in AD.  (+info)

In vitro reactions of butadiene monoxide with single- and double-stranded DNA: characterization and quantitation of several purine and pyrimidine adducts. (3/1399)

We have previously shown that butadiene monoxide (BM), the primary metabolite of 1,3-butadiene, reacted with nucleosides to form alkylation products that exhibited different rates of formation and different stabilities under in vitro physiological conditions. In the present study, BM was reacted with single-stranded (ss) and double-stranded (ds) calf thymus DNA and the alkylation products were characterized after enzymatic hydrolysis of the DNA. The primary products were regioisomeric N-7-guanine adducts. N-3-(2-hydroxy-3-buten-1-yl)adenine and N-3-(1-hydroxy-3-buten-2-yl)adenine, which were depurinated from the DNA more rapidly than the N-7-guanine adducts, were also formed. In addition, N6-(2-hydroxy-3-buten-1-yl)deoxyadenosine and N6-(1-hydroxy-3-buten-2-yl)deoxyadenosine were detected and evidence was obtained that these adducts were formed by Dimroth rearrangement of the corresponding N-1-deoxyadenosine adducts, not while in the DNA, but following the release of the N-1-alkylated nucleosides by enzymatic hydrolysis. N-3-(2-hydroxy-3-buten-1-yl)deoxyuridine adducts, which were apparently formed subsequent to deamination reactions of the corresponding deoxycytidine adducts, were also detected and were stable in the DNA. Adduct formation was linearly dependent upon BM concentration (10-1000 mM), with adduct ratios being similar at the various BM concentrations. At a high BM concentration (750 mM), the adducts were formed in a linear fashion for up to 8 h in both ssDNA and dsDNA. However, the rates of formation of the N-3-deoxyuridine and N6-deoxyadenosine adducts increased 10- to 20-fold in ssDNA versus dsDNA, whereas the N-7-guanine adducts increased only slightly, presumably due to differences in hydrogen bonding in ssDNA versus dsDNA. These results may contribute to a better understanding of the molecular mechanisms of mutagenesis and carcinogenesis of both BM and its parent compound, 1,3-butadiene.  (+info)

Cytotoxic effect of paraquat on rat C6 glioma cells: evidence for the possibility of non-oxidative damage to the cells. (4/1399)

Although paraquat has been shown to cause oxidative damage to neuronal cells, little is known about its effect on glial cells. Thus the effect of paraquat on glial cells was examined using rat C6 glioma cells as a model system. Paraquat reduced cell viability in a concentration- and time-dependent manner, and this toxic effect was not significantly attenuated by various kinds of antioxidants. Furthermore, paraquat failed to increase 8-hydroxy-deoxyguanosine formation in the cells. These results indicate that paraquat can be toxic to glial cells and suggest that this cytotoxic effect may not be associated with the oxidative damage to the cells.  (+info)

Age-associated increase in 8-oxo-deoxyguanosine glycosylase/AP lyase activity in rat mitochondria. (5/1399)

The mitochondrial theory of aging postulates that organisms age due to the accumulation of DNA damage and mutations in the multiple mitochondrial genomes, leading to mitochondrial dysfunction. Among the wide variety of DNA damage, 8-oxo-deoxyguanosine (8-oxo-dG) has received the most attention due to its mutagenicity and because of the possible correlation between its accumulation and pathological processes like cancer, degenerative diseases and aging. Although still controversial, many studies show that 8-oxo-dG accumulates with age in the mitochondrial (mt) DNA. However, little is known about the processing of this lesion and no study has yet examined whether mtDNA repair changes with age. Here, we report the first study on age-related changes in mtDNA repair, accomplished by assessing the cleavage activity of mitochondrial extracts towards an 8-oxo-dG-containing substrate. In this study, mitochondria obtained from rat heart and liver were used. We find that this enzymatic activity is higher in 12 and 23 month-old rats than in 6 month-old rats, in both liver and heart extracts. These mitochondrial extracts also cleave oligonucleotides containing a U:A mismatch, at the uracil position, reflecting the combined action of mitochondrial uracil DNA glycosylase (mtUDG) and mitochondrial apurinic/apyrimidinic (AP) endonucleases. The mtUDG activity did not change with age in liver mitochondria, but there was a small increase in activity from 6 to 23 months in rat heart extracts, after normalization to citrate synthase activity. Endonuclease G activity, measured by a plasmid relaxation assay, did not show any age-associated change in liver, but there was a significant decrease from 6 to 23 months in heart mitochondria. Our results suggest that the mitochondrial capacity to repair 8-oxo-dG, the main oxidative base damage suggested to accumulate with age in mtDNA, does not decrease, but rather increases with age. The specific increase in 8-oxo-dG endonuclease activity, rather than a general up-regulation of DNA repair in mitochondria, suggests an induction of the 8-oxo-dG-specific repair pathway with age.  (+info)

Hyperglycemia causes oxidative stress in pancreatic beta-cells of GK rats, a model of type 2 diabetes. (6/1399)

Reactive oxygen species are involved in a diversity of biological phenomena such as inflammation, carcinogenesis, aging, and atherosclerosis. We and other investigators have shown that the level of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a marker for oxidative stress, is increased in either the urine or the mononuclear cells of the blood of type 2 diabetic patients. However, the association between type 2 diabetes and oxidative stress in the pancreatic beta-cells has not been previously described. We measured the levels of 8-OHdG and 4-hydroxy-2-nonenal (HNE)-modified proteins in the pancreatic beta-cells of GK rats, a model of nonobese type 2 diabetes. Quantitative immunohistochemical analyses with specific antibodies revealed higher levels of 8-OHdG and HNE-modified proteins in the pancreatic beta-cells of GK rats than in the control Wistar rats, with the levels increasing proportionally with age and fibrosis of the pancreatic islets. We further investigated whether the levels of 8-OHdG and HNE-modified proteins would be modified in the pancreatic beta-cells of GK rats fed with 30% sucrose solution or 50 ppm of voglibose (alpha-glucosidase inhibitor). In the GK rats, the levels of 8-OHdG and HNE-modified proteins, as well as islet fibrosis, were increased by sucrose treatment but reduced by voglibose treatment. These results indicate that the pancreatic beta-cells of GK rats are oxidatively stressed, and that chronic hyperglycemia might be responsible for the oxidative stress observed in the pancreatic beta-cells.  (+info)

Glutathione and ascorbate are negatively correlated with oxidative DNA damage in human lymphocytes. (7/1399)

Intracellular antioxidants, glutathione and ascorbate, and two molecular markers of oxidative DNA damage, 5-hydroxy-2'-deoxycytidine (5-OH-dCyd) and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo), were measured in lymphocytes from 105 healthy volunteers. The analysis of 5-OH-dCyd and 8-oxo-dGuo was carried out by HPLC with electrochemical detection such that both compounds were detected on the same chromatography run. There was no significant difference in oxidative DNA damage when the extraction of DNA from cells using phenol was carried out under anaerobic conditions or in the presence of metal ion chelators. This indicates that auto-oxidation of DNA during sample preparation was minimal. Using the above methods, the average level of oxidative DNA damage in lymphocytes was 2.9 +/- 1.4 for 5-OH-dCyd and 4.5 +/- 1.8 for 8-oxo-dGuo lesions per 10(6) dGuo (n = 105). It is unlikely that artifactual oxidation contributed to the observed damage because the level of 5-OH-dCyd was comparable with that of 8-oxo-dGuo in lymphocyte DNA, whereas 8-oxo-dGuo outnumbers 5-OH-dCyd by a ratio of >5:1 when DNA is exposed to various oxidants, including ionizing radiation or Fenton reagents. Rather, the nearly equal levels of 5-OH-dCyd and 8-oxo-dGuo in cellular DNA implies that 8-oxo-dGuo may be more efficiently removed by DNA repair. Finally, and most importantly, the correlation of our endpoints revealed that the naturally occurring level of intracellular antioxidants was negatively correlated to the level of oxidative DNA damage with the strongest correlation observed for glutathione and 8-oxo-dGuo (r = -0.36; P < 0.001). These results strongly suggest that intracellular glutathione and ascorbate protect human lymphocytes against oxidative DNA damage.  (+info)

Mutagenic properties of the 8-amino-2'-deoxyguanosine DNA adduct in mammalian cells. (8/1399)

The DNA adduct 8-amino-2'-deoxyguanosine (8-amino-dG) is found in liver DNA of rats treated with the hepatocarcinogen 2-nitropropane. Site-specifically modified oligodeoxynucleotides were used to explore the mutagenic potential of 8-amino-dG in simian kidney (COS-7) cells. Oligodeoxynucleotides (5'-TCCTCCTX1G2CCTCTC and 5'-TCCTCCTG1X2CCTCTC, X = dG or 8-amino-dG) with the lesion positioned at codon 60 or 61 of the non-coding strand of the human c-Ha- ras1 gene were inserted into single-stranded phagemid vectors and transfected into COS-7 cells. The progeny plasmid obtained was used to transform Escherichia coli DH10B. Transformants were analyzed by oligodeoxynucleotide hybridization and DNA sequencing to establish the mutation frequency and spectrum produced by the modified base. The correct base, dCMP, was incorporated preferentially opposite 8-amino-dG at X1and X2. When 8-amino-dG was at X1, targeted GNH2-->T transversions were detected, along with smaller numbers of GNH2-->A transitions and GNH2-->C transversions. When the adduct was at X2, only GNH2-->T transversions were observed. The frequencies of targeted mutation at X1and X2were 2.7 and 1.7%, respectively. Mutation frequency and mutagenic spectrum were sequence context dependent. In addition, non-targeted G-->T transversions, accompanied by some G-->A transitions, were detected 5' to 8-amino-dG when the lesion was at X2. We conclude that 8-amino-dG is a mutagenic lesion, generating G-->T and G-->C transversions and G-->A transitions in mammalian cells.  (+info)

TY - JOUR. T1 - Effects of genetic polymorphisms in metabolic enzymes on the relationships between 8-hydroxydeoxyguanosine levels in human leukocytes and urinary 1-hydroxypyrene and 2-naphthol concentrations. AU - Kim, Yong Dae. AU - Lee, Chul Ho. AU - Nan, Hong Mei. AU - Kang, Jong Won. AU - Kim, Heon. PY - 2003/5/1. Y1 - 2003/5/1. N2 - This study was designed to investigate the relationship between environmental exposure to polycyclic aromatic hydrocarbons (PAHs) and oxidative stress, and to evaluate the effects of cigarette smoking and the genetic polymorphisms of CYP1A1, CYP2E1, GSTM1, NAT2 and UGT1A6 on the relationship. The subjects of this study were 105 healthy Korean males without occupational exposure to PAHs. The 8-hydroxydeoxyguanosine (8-OHdG) level in leukocytes, and urinary 1-hydroxypyrene (1-OHP) and 2-naphthol concentrations, were measured by high-performance liquid chromatography. Genetic polymorphisms of CYP1A1, CYP2E1, GSTM1, NAT2 and UGT1A6 were identified by PCR and ...
Deoxyguanosine kinase (ATP: deoxyguanosine 5-phosphotransferase) activity has been identified in neonatal mouse skin tissue. This activity which has a molecular weight of 44 000 was shown to be highly specific for deoxyguanosine as a substrate. Unique to deoxynucleoside kinases was the observation that this enzyme possessed a pH optimum of 5.2. In dilute solutions catalytic activity is lost, however the enzymatic activity can be stabilized by the addition of 20 micrometer MgATP or ATP. Kinetic analysis gave an apparent Km for deoxyguanosine of 7 micrometer. Double-reciprocal plots of activity vs. MgATP concentration produced a broken line with the break occurring at 0.5 mM MgATP. Below this concentration an apparent Km for MgATP of 23 micrometer was measured; above 0.5 mM MgATP an apparent Km of 265 micrometer was calculated. Mouse skin deoxyguanosine kinase was strongly inhibited by dGTP, dGDP and UDp. dGTP was a competitive inhibitor of deoxyguanisine with an apparent Ki of 1.9 micrometer. UPD (K1
2-Hexenal is an α,β-unsaturated carbonyl compound which is mutagenic, genotoxic and forms cyclic 1,N2-propanodeoxyguanosine adducts like similar propenals for which carcinogenicity was shown, e.g. acrolein or crotonaldehyde. Since humans have a permanent intake of 2-hexenal via vegetarian food this genotoxic compound is considered to play a role in human carcinogenicity. The data base is, however, presently not sufficient for a cancer risk assessment. To date no long term carcinogenicity study on 2-hexenal has been published. Detection of respective DNA adducts of this substance in animals or humans could allow cancer risk assessment. Therefore, we have developed a 32P-post-labeling technique based on nuclease P1 enrichment and TLC separation of the labeled adducts. The respective adducts are stable over a wide pH range from pH 4 to pH 11 and relatively stable against nuclease P1. The detection limit was 0.03 adducts per 106 nucleotides and the recovery was 10%. With this method we have shown ...
There is extensive experimental evidence that oxidative damage permanently occurs to lipids of cellular membranes, proteins, and DNA. In nuclear and mitochondrial DNA, 8-hydroxy-2 -deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2 -deoxyguanosine (8-oxodG) is one of the predominant forms of free radical-induced oxidative lesions, and has therefore been widely used as a biomarker for oxidative stress and carcinogenesis. Studies showed that urinary 8-OHdG is a good biomarker for risk assessment of various cancers and degenerative diseases. The most widely used method of quantitative analysis is high-performance liquid chromatography (HPLC) with electrochemical detection (EC), gas chromatography-mass spectrometry (GC-MS), and HPLC tandem mass spectrometry. In order to resolve the methodological problems encountered in measuring quantitatively 8-OHdG, the European Standards Committee for Oxidative DNA Damage was set up in 1997 to resolve the artifactual oxidation problems during the
Cisplatin is reported to increase intracellular ROS and 8-oxodG levels (27). As Fpg and α-OGG1 overexpression has been shown to lower 8-oxodG and oxypurine-clustered DNA damage levels, and OGG1-deficient mice exhibit tissue-specific increases in 8-oxodG accumulation following ROS-inducing xenobiotic exposure (12-16, 55), we measured nuclear 8-oxodG/dG ratios in untreated and drug-treated cell clones (Fig. 5E and F). Ectopic expression of Fpg (clone fpg 6a) or α-OGG1 (clone OGG1 1d) significantly lowered endogenous nuclear 8-oxodG levels compared with the pC 1c clone control. In addition, stable transgene expression markedly inhibited an immediate increase in 8-oxodG generation following cisplatin treatment (30 μmol/L; Fig. 5E). A maximal 1.5-fold increase in 8-oxodG/dG occurred 30 minutes after treatment compared with untreated sample and was diminished by 2 hours after treatment. Oxaliplatin exposure produced a slightly lower increase in 8-oxodG/dG that also was inhibited in fpg and α-OGG1 ...
Oxidative damage of DNA has been implicated as a fundamental cause of the physiologic changes and degenerative diseases associated with aging. When DNA is impacted by oxidative stress, the chemical 8-Oxo-2′-deoxyguanosine (8-oxo-dG) is produced as a byproduct.. Becasue 8-oxo-dG is a major product of DNA oxidation, concentrations of 8-oxo-dG within a cell is a ubiquitous marker and measurement of oxidative stress. 8-oxo-dG increases with age in DNA of mammalian tissues. 1 8-oxo-dG increases in both mitochonndrial DNA and nuclear DNA with age. 2 DNA is probably the most biologically significant target of oxidative attack and may be implicated in aging, carcinogenesis and other degenerative diseases. Environmental factors, lifestyle choices such as smoking and recreational drugs, and some pharmaceuticals have also been associated with elevated urine levels of 8-oxo-dG. For example, according to multiple regression analysis, smokers excreted 50% (31-69%; 95% confidence interval) more 8-oxo-dG than ...
Anticancer therapy with cisplatin and oxaliplatin is limited by toxicity and onset of tumor resistance. Both drugs form platinum-DNA cross-linked adducts, and cisplatin causes oxidative DNA damage including the 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxodG) lesion. To assess oxidative DNA damage as a mechanism of cisplatin and oxaliplatin cytotoxicity, 8-oxodG-directed base excision repair was stably enhanced in human embryonic kidney cells by FLAG-tagged expression of human oxoguanine glycosylase 1 (α-OGG1) or its functional homologue, Escherichia coli formamidopyrimidine glycosylase (fpg). Both drugs increased reactive oxygen species and 8-oxodG levels, and cytotoxicity was decreased by antioxidant pretreatment. Ectopic expression of α-OGG1 or fpg in cell clones increased nuclear and mitochondrial 8-oxodG repair, and reduced death by reactive oxygen species initiators (H2O2, menadione) and both platinum drugs. Exposure to oxaliplatin caused a more marked and sustained block of cell ...
8-Hydroxydeoxyguanosine (8-OHdG) is a major oxidative DNA adduct playing roles in senescence, carcinogenesis and various disease processes. High-performance liquid chromatography with an electrochemical detection (HPLC-ECD) method has been widely used to assess organ levels of 8-OHdG, and a recently introduced immunohistochemical approach has made it possible to clarify intra-organ localization. In the present study, these methods were employed to reveal age-dependent changes in nuclear 8-OHdG within various tissues of male Fischer 344 rats between 18 fetal days and 104 weeks of age. 8-OHdG was detected in the nuclei of cerebellar small granule and small cortical cells, cerebral nerve cells, and choroid plexus epithelia of the brain and ependymal cells of the spinal cord; parenchymal cells in the anterior lobe of the pituitary and adrenal glands (mainly cortex); bronchial epithelium of the lung; intra-hepatic bile duct, pancreatic duct, glandular gastric and intestinal epithelial cells; renal tubular
Global Deoxyguanosine Market - Global & Regional Industry Coverage from 2020 to 2027 Summary of the Report Growth of the Deoxyguanosine market
Free 8-hydroxydeoxyguanosine (oh(8)dG), a nucleoside of 8-hydroxyguanine (oh(8)Gua), present in cytosol is not incorporated into DNA. However, nothing is known about its biological function when it presents in cytosol as a free form. We demonstrate here for the first time that oh(8)dG inhibits lipopolysaccharide (LPS)-induced nitric oxide (NO) production and cyclooxygenase-2 (COX-2) activity, and both gene transcriptions in microglia. Furthermore, oh(8)dG reduced mRNA levels of pro-inflammatory cytokine, such as IL-1beta, IL-6, and TNF-alpha, in activated BV2 cells. We also found that oh(8)dG suppressed reactive oxygen species (ROS) production through reduction of NADPH oxidase activity and blocked Rac1/STATs signal cascade. Finally, oh(8)dG suppressed recruitment of STATs and p300 to the iNOS and COX-2 promoters, and inhibited H3 histone acetylation. Taken together, these results provide new aspects of oh(8)dG as an anti-inflammatory agent ...
Reactive oxygen species (ROS) induced damage to DNA plays a major role in carcinogenesis. In order to estimate the level of oxidative damage and its role in breast cancer, 8-hydroxy-2-deoxyguanosine (8-OHdG) was determined in DNA isolated from human breast tissue. Furthermore, we investigated wheth …
Evans, M.D., Cooke, M.S., Olinski, R., Gackowski, D., Rozalski, R., Siomek, A., Loft, S., Rossner, Jr. P., Sram, R., Henriksen, T., Poulsen, H.E., Weimann, A., Barbieri, A., Sabatini, L., Violante, F., Kino, S., Ochi, T., Sakai, K., Takeuchi, M., Kasai, H., Meerman, J.H.N., Halliwell, B., Jenner, A.M., Wang, H., Cerda, C., Saez, G., Haghdoost, S., Svoboda, P., Hu, C.-W., Chao, M.-R., Peng, K.-Y., Shih, W.-C., Wu, K.-Y., Orhan, H., Istanbullu, N.S., Mistry, V., Farmer, P.B., Sandhu, J., Singh, R., Cortez, C., Su, Y., Santella, R.M., Lambert, P., Smith, R. (2010). Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2′- deoxyguanosine as a noninvasive biomarker of oxidative stress. FASEB Journal 24 (4) : 1249-1260. [email protected] Repository. https://doi.org/10.1096/fj.09- ...
In the present study, we compared levels of oxidative stress using urinary levels of 15-F2t-IsoP and 8-oxodG among breast cancer cases and controls. We found significantly elevated ORs for breast cancer risk when 15-F2t-IsoP levels were categorized based on quartiles in controls (Table 1). This relationship was found even when those women who had received radiation therapy, which was found to significantly increase 15-F2t-IsoP levels, were removed from the analysis. Radiation treatment has been shown previously to result in increased levels of urinary 8-oxodG (19, 20). There was no significant relationship with 8-oxodG levels when all cases were included in the analysis. However, removal of women with radiation therapy from the analysis resulted in a significant trend of decreased risk with higher levels of urinary 8-oxodG. Although the assay variability was 25%, we still had excellent power to detect case control differences of an OR ≤0.6 or ,1.8.. Several studies have provided evidence that ...
Background: Cigarette smoking and tobacco chewing are common modes of consuming tobacco all overthe world. Parents need to be aware that germ cell integrity is vital for birth of healthy offspring as biologicalparenting begins much before birth of a child and even before conception. The present study was conducted todetermine the etiology of non-familial sporadic heritable retinoblastoma (NFSHRb), by evaluating oxidativesperm DNA damage in fathers due to use of tobacco (smoking and chewing). Materials and Methods: We recruited145 fathers of NFSHRb children and 53 fathers of healthy children (controls) in the study. Tobacco history wasobtained by personal interview. Seminal reactive oxygen species (ROS) in semen, sperm DNA fragmentationindex (DFI) and 8 hydroxy 2 deoxyguanosine (8-OHdG) levels in sperm were evaluated. The RB1 gene wasscreened in genomic blood DNA of parents of children with NFSHRb and controls. Odds ratios (ORs) derivedfrom conditional logistic regression models. Results: There was
Deoxyguanosine (dG) nucleoside molecule. DNA (deoxyribonucleic acid) building block. Atoms are represented as spheres with conventional colour coding: hydrogen (white), carbon (grey), nitrogen (blue), oxygen (red). - Stock Image F010/6822
Global Deoxyguanosine Market by Manufacturers, Countries, Type and Application, Forecast to 2022 published in Aug 2017 spread across 122 pages, providing competitive landscape analysis, market share info and more is now available for US $3480 at Market Research Reports.
The lung has been shown to be a target organ for the effects of Benzo[a]pyrene (B[a]P). 8-hydroxy-2-deoxyguanosine (oxo8dG) is a mutagenic oxidized base formed in DNA during the metabolism of B[a]P by the peroxidase and prostaglandin H synthetase systems. The objective of this study was to determine the capacity to cleave oxo8dG in the lung, liver, and kidney by measuring the activity of 8-oxogua
Chemical Names: 2-Deoxyguanosine Catalog Number: 07-4000 CAS Number: 961-07-9 Molecular Formula: 285.20 Molecular Weight: C10H13N5O4 H2O
You are viewing an interactive 3D depiction of the molecule 8-(4-amino-4-biphenylyl)-2-deoxyguanosine (C22H23N6O4) from the PQR.
[65 Pages Report] Check for Discount on N2-Isobutyryl-2-Fluoro-2-deoxyguanosine Global Market and Forecast Research report by ChemReport. DescriptionWe provide independent and unbiased information on manufacturers, prices, production...
Gentaur molecular products has all kinds of products like :search , Trevigen \ 8-oxo-dG Elisa Kit \ 4380-096-K for more molecular products just contact us
Administration of 1′-[2′,3′-3H]hydroxysafrole to adult female mice resulted in the formation of DNA-, ribosomal RNA-, and protein-bound adducts in the liver that reached maximum levels within 24 hr. The levels of all three macromolecule-bound adducts decreased rapidly between 1 and 3 days after injection, at which time the amounts of the DNA-bound adducts essentially plateaued at approximately 15% of the maximum level. The amounts of the protein and ribosomal RNA adducts were very low by 20 days.. Comparison by high-performance liquid chromatography of the deoxyribonucleoside adducts obtained from the hepatic DNA with those formed by reaction of deoxyguanosine and deoxyadenosine with 1′-acetoxysafrole, 1′-hydroxysafrole-2′,3′-oxide, and 1′-oxosafrole indicated that the four in vivo adducts studied were derived from an ester of 1′-hydroxysafrole. Three of the four in vivo adducts comigrated with adducts formed by reaction of 1′-acetoxysafrole with deoxyguanosine; the fourth ...
You can download our 2017 product catalog in various formats, request to have a hard copy sent to you, or view our products online. ...
In this study, the ELISA estimate was similar to the HPLC estimate on purified 8-OH-dG fractions from 23 urine samples. A good correlation (r = 0.833; P , 0.0001) was observed between the two estimates. It has been reported that the mean value of 8-0H-dG in human urine samples determined by HPLC-ECD (this study), HPLC-ECD (another group; Ref. 17 ), gas chromatography with mass spectrometric detection (11) , and HPLC-ELISA (this study) was 5.47 ± 2.97 (mean ± SD), 3.68-3.96, 3.33-3.95, and 5.50 ± 2.36 (mean ± SD) μg/g creatinine, respectively. These results indicate that the monoclonal antibody N45.1 used for a commercial ELISA kit is quite specific for 8-OH-dG.. However, the ELISA estimate on 120 original urine samples (9.33 ± 3.23 μg/g creatinine) was ∼2-fold higher than the HPLC estimate (4.46 ± 2.03 μg/g creatinine). A lower correlation was observed between the two estimates compared with the values on the purified 8-OH-dG fractions. Ten % of urine samples showed a high ratio of ...
Affiliation:大分大学,教育福祉科学部,准教授, Research Field:Public health/Health science,Public health/Health science,Applied health science,Applied health science, Keywords:尿中8-Hydroxydeoxyguanosine,Reactive Oxygen Metabolites,生体内酸化ストレス,尿中7-Methyleguanine,Biological Antioxygen Potentials,分子疫学,8-OH-dG,生活習慣,思春期,学校保健, # of Research Projects:5, # of Research Products:35, Ongoing Project:前駆脂肪細胞分化誘導能レベルによる思春期の健康評価と予後推定
This modification is available as part of a custom oligonucleotide configuration. Please use OLIGObuilder™ to generate pricing and order or contact us to speak with a customer service representative. ...
Your browser does not support JavaScript or is for some reason turned off! Please note that this website will not display correctly ...
* found in: NADP Disodium Salt Trihydrate, DNTP Set, 2-Deoxyguanosine Monohydrate, Adenosine-5-Triphosphate (ATP), Uracil, DATP 100 mm Solution pH 7.0,..
HU-1C, 2B, 3A, 3C, 3G, 4A, 4B, 6A, 7A, 8A, 8B, 8C, 9A, 9C, 10A, 10B, 10C, 11A, 11B, 11D, 12A, 12B, 12C, 13A, 14A, 14C, 15A, 15D, 15E, 16A, 16B, 17A, 18A, 18C,19F, 20A, 24A, 24B, 24C, 25A, 25C, 26A, 26B, 27A, 28A, 29A, 30A, 32A, 32B, 33C, 34A, 34B, 35C, 36B, 37A, 37B, 37C, 38A, 39B, 40A, 40B, 40C, 40D, 42A, 43A, 44A, 45A, 46A, 47A, 48A, 50A, 51A, 51B, 52A, 52C, 53A, 54A, 54B, 54E, 54F, 57B, 57C, 58A, 58B, 58C, 59A, 60A, 61A, 62A, 62B, 63A, 63B, 63C, 64A, 64C, 64D, 65B, 65C, 66A, 67A, 67B, 68A, 69A, 70A, 70B ...
Oxidative stress is associated with the development of various diseases including cancer, arteriosclerosis, diabetes mellitus, hypertension and metabolic syndrome. However, little is known about the involvement of 8-hydroxydeoxyguanosine (8-OHdG) dur
Research has indicated that oxidative stress is the cause of many serious diseases such as cancer, Alzheimers, arteriosclerosis and diabetes.
2-Deoxyguanosine hydrate, 99+%, ACROS Organics™ 1g; Glass bottle 2-Deoxyguanosine hydrate, 99+%, ACROS Organics™ Carbonyl Compounds
We characterized the oxidative stress (OS) status by the levels of reduced/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA) and the mutagenic base 8-oxo-78-dihydro-2-deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same patients. We also analyzed 8-oxo-dG in peripheral mononuclear cells (PMNC) and urine from healthy control subjects and in affected patients in the basal state and one, three, six, nine and twelve months after tumor resection. The levels of DNA repair enzyme mRNA expression (hOGG1, RAD51, MUYTH and MTH1) were determined in tumor specimens and compared with normal mucosa. Tumor specimens exhibited increased levels of MDA and 8-oxo-dG compared with normal gastric tissue. GSH levels were also increased, while GSSG levels remained stable. DNA repair enzyme mRNA expression was induced in the tumor tissues. Levels of 8-oxo-dG were significantly elevated in both urine and PMNC of gastric cancer patients compared with
There is increasing experimental evidence to indicate that O6-methyldeoxyguanosine (O6-MedG) formation in DNA is a critical cytotoxic event following exposure to certain antitumor alkylating agents and that the DNA repair protein O6-alkylguanine-DNA-alkyltransferase (ATase) can confer resistance to these agents. We recently demonstrated a wide interindividual variation in the depletion and subsequent regeneration of ATase in peripheral blood lymphocytes of patients treated with 24-h continuous infusion of 1-p-carboxyl-3,3-dimethylphenyltriazene (CB10-277) for metastatic melanoma. We have now measured the formation of O6-MedG in the DNA of peripheral leukocytes of nine patients receiving this treatment regimen. This lesion could be detected in DNA within 1 h and a progressive increase in adduct levels occurred during the CB10-277 infusion and for 24 h after completion. Considerable interindividual variation was observed in the peak O6-MedG levels, with values ranging from 3.0 to 23.8 µmol ...
TY - JOUR. T1 - Methylation of 2′-deoxyguanosine by a free radical mechanism. AU - Crean, Conor. AU - Geacintov, Nicholas. AU - Shafirovich, Vladimir. PY - 2009/9/24. Y1 - 2009/9/24. N2 - The mechanistic aspects of the methylation of guanine in DNA initiated by methyl radicals that are derived from the metabolic oxidation of some chemical carcinogens remain poorly understood. In this work, we investigated the kinetics and the formation of methylated guanine products by two methods: (i) the combination of •CH3 radicals and guanine neutral radicals, G(-H)•, and (ii) the direct addition of •CH3 radicals to guanine bases. The simultaneous generation of •CH3 and dG(-H)• radicals was triggered by the competitive oneelectron oxidation of dimethyl sulfoxide (DMSO) and 2-deoxyguanosine (dG) by photochemically generated sulfate radicals in deoxygenated aqueous buffer solutions (pH 7.5). The photolysis of methylcob(III)alamin to form •CH3 radicals was used to investigate the direct addition ...
TY - JOUR. T1 - Comparison of urinary levels of 8-hydroxy-2-deoxyguanosine between young females with and without depressive symptoms during diferent menstrual phases. AU - Iida, Tadayuki. AU - Inoue, Ken. AU - Ito, Yasuhiro. AU - Ishikawa, Hiroaki. AU - Kagiono, Miwa. AU - Teradaira, Ryoji. AU - Chikamura, Chiho. AU - Harada, Toshihide. AU - Ezoe, Satoko. AU - Yatsuya, Hiroshi. PY - 2015. Y1 - 2015. N2 - This study aimed to clarify the association between depressive symptoms and a marker of oxidative stress-induced DNA damage in young females. Since the menstrual cycle may confound or modify this association, depressive symptoms and urinary levels of 8-hydroxy-2 deoxyguanosine (8-OHdG) were evaluated during each menstrual phase. A total of 57 female fourth-year students (aged 21.6 ± 0.8) from a Japanese health science university were studied. The menstrual cycle was divided into 3 phases: menstrual (days 1 to 3 after the onset of menses); proliferative (days 13 to 15); and secretory (days 24 ...
8-hydroxy-2-deoxyguanosine ELISA kit is a sandwich enzyme immunoassay for the quantitative measurement of 8-hydroxy-2-deoxyguanosine. (KA0444) - Products - Abnova
Objective: To investigate potential mechanisms of oxidative DNA damage in a rat model of type I diabetes and in murine proximal tubular epithelial cells (MCT) and primary culture of rat proximal tubular epithelial cells (RPTE).. Research Design and Methods: Phosphorylation of Akt and tuberin, 8-oxodG levels and OGG1 expression were measured in kidney cortical tissue of control and type I diabetic animals as well as in proximal tubular cells incubated with normal or high glucose.. Results: In the renal cortex of diabetic rats, the increase in Akt phosphorylation is associated with enhanced phosphorylation of tuberin, decreased OGG1 protein expression and 8-oxodG accumulation. Exposure of proximal tubular epithelial cells to high glucose (HG) causes a rapid increase in ROS generation that correlates with the increase in Akt and tuberin phosphorylation. HG also resulted in downregulation of OGG1 protein expression, paralleling its effect on Akt and tuberin. Inhibition of PI-3K/Akt significantly ...
Sigma-Aldrich offers Sigma-I6508, N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine 3′-O-succinic acid for your research needs. Find product specific information including CAS, MSDS, protocols and references.
Our results indicate that serum 8-OHdG is increased already in prediabetes suggesting oxidative DNA damage to be present with minor elevation of blood glucose levels (BGLs). The statistically significant positive correlation between serum 8-OHdG and body mass index in the diabetic group indicates that obesity has an additive effect to increased BGL contributing to oxidative DNA damage ...
List of 10 disease causes of Intermittent high cholesterol, patient stories, diagnostic guides. Diagnostic checklist, medical tests, doctor questions, and related signs or symptoms for Intermittent high cholesterol.
The amount of work dedicated to the study of DNA oxidative damage is large enough tosuccessfully compete with the studies on lipid peroxidation (Chapters 25 and
1LAS: Structure of an oligodeoxynucleotide containing a 1,N(2)-propanodeoxyguanosine adduct positioned in a palindrome derived from the Salmonella typhimurium hisD3052 gene: Hoogsteen pairing at pH 5.2.
1LAE: Structure of the 1,N(2)-propanodeoxyguanosine adduct in a three-base DNA hairpin loop derived from a palindrome in the Salmonella typhimurium hisD3052 gene.
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
The IUPHAR/BPS Guide to Pharmacology. DG-051 ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.
In this study, WANG Yanlis lab in IBP and collaborators from Netherlands demonstrated that TtAgo can independently generate and selectively load functional DNA guides. They found that the guide-free TtAgo is able to degrade unstable double-stranded DNA (dsDNA) targets, generating short DNA products, which are selectively loaded onto TtAgo and guide subsequent DNA target cleavage. They also showed that TtAgo loads dsDNA molecules with a preference toward a deoxyguanosine on the passenger strand at the position opposite to the 5′ end of the guide strand. This explains why in vivo TtAgo is preferentially loaded with guides with a 5′ end deoxycytidine. ...
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
Deoxyguanosine triphosphate, labeled on the alpha phosphate group with 32P. For applications such as DNA labeling, DNA sequencing, random priming, nick translation, and labeling.
Ime (up to 72 h) and analyzed by 223488-57-1 custom synthesis HPLC-ECD and LC/MS. Figure 7 shows the representative HPLC chromatograms of TFDG incubated with
Learn more about 3-methyl-2-oxo-2-3-dihydro-1h-imidazole-4-carboxylic-acid. We enable science by offering product choice, services, process excellence and our people make it happen.
Learn more about 2-oxo-2-3-trifluoromethyl-benzyl-amino-ethyl-phenyl-amino-acetic-acid. We enable science by offering product choice, services, process excellence and our people make it happen.
... deoxyguanosine kinase". Johansson M, Karlsson A (1996). "Cloning and expression of human deoxyguanosine kinase cDNA". ... Deoxyguanosine kinase, mitochondrial is an enzyme that in humans is encoded by the DGUOK gene. In mammalian cells, the ... Taanman JW, Kateeb I, Muntau AC, Jaksch M, Cohen N, Mandel H (2002). "A novel mutation in the deoxyguanosine kinase gene ... Lotfi K, Månsson E, Peterson C, Eriksson S, Albertioni F (2002). "Low level of mitochondrial deoxyguanosine kinase is the ...
Hurley MC, Palella TD, Fox IH (December 1983). "Human placental deoxyadenosine and deoxyguanosine phosphorylating activity". ... and deoxyguanosine (dG), and convert them into their monophosphate forms. There has been recent biomedical research interest in ...
8-Oxo-2'-deoxyguanosine Cattley RC, Glover SE (1993). "Elevated 8-hydroxydeoxyguanosine in hepatic DNA of rats following ...
Purification and properties of deoxyguanosine monophosphokinase of calf thymus". Eur. J. Biochem. 19 (2): 256-63. doi:10.1111/j ...
... is a synthetic analogue of 2′-deoxy-guanosine. It is first phosphorylated to ganciclovir monophosphate by a viral ... Ganciclovir triphosphate is a competitive inhibitor of deoxyguanosine triphosphate (dGTP) incorporation into DNA and ...
10-epoxide deoxyguanosine adducts". J. Biol. Chem. 277 (14): 11765-71. doi:10.1074/jbc.M112139200. PMID 11821420. Kusumoto R, ... "Mammalian translesion DNA synthesis across an acrolein-derived deoxyguanosine adduct. Participation of DNA polymerase eta in ...
... deoxyguanosine". Proc. Natl. Acad. Sci. U.S.A. 104 (41): 16092-7. doi:10.1073/pnas.0705884104. PMC 1999398 . PMID 17911257. ...
Other names in common use include deoxy-GTPase, deoxyguanosine 5-triphosphate triphosphohydrolase, deoxyguanosine ... "Enzymatic cleavage of deoxyguanosine triphosphate to deoxyguanosine and tripolyphosphate". J. Biol. Chem. 233 (1): 159-62. PMID ... SETO D, BHATNAGAR SK, BESSMAN MJ (1988). "The purification and properties of deoxyguanosine triphosphate triphosphohydrolase ... deoxyguanosine + triphosphate Thus, the two substrates of this enzyme are dGTP and H2O, whereas its two products are ...
8-Oxo-2'-deoxyguanosine 8-hydroxyguanine - Compound Summary, PubChem Kanvah, S.; et al. (2010). ", Oxidation of DNA: Damage to ... Kasai, H (December 1997). "Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular ... deoxyguanosine: an isotope based method to evaluate artefactual oxidation of DNA during its extraction and subsequent work-up ...
... as well as for deoxyguanosine). The thermodynamic stability of a wobble base pair is comparable to that of a Watson-Crick base ...
In a DNA double helix, it will base pair with deoxyguanosine monophosphate.. ...
Schwalbe, H; Wacker A; Buck J; Richter C; Wohnert J (2011). "Structure and dynamics of the deoxyguanosine-sensing riboswitch ...
... deoxyguanosine 5'-triphosphate (dGTP) induces reduction of adenosine 5'-diphosphate (ADP); and 2'-deoxythymidine 5'- ...
... deoxyguanosine in human cells". J. Am. Chem. Soc. 133 (24): 9140-3. doi:10.1021/ja2004686. PMID 21604744. Tompkins EM, McLuckie ...
Levels of 8-oxo-2'-deoxyguanosine (8-OHdG), one of the predominant forms of free radical-induced oxidative DNA damages, are ... Valavanidis A, Vlachogianni T, Fiotakis C (2009). "8-hydroxy-2' -deoxyguanosine (8-OHdG): A critical biomarker of oxidative ...
The N(2)-Furfuryl-deoxyguanosine Adduct Does Not Alter the Structure of B-DNA. J Org Chem. 2016 Jan 15;81(2):502-11. doi: ... Nair DT, Kottur J, Sharma R. A rescue act: Translesion DNA synthesis past N(2) -deoxyguanosine adducts. IUBMB Life. 2015 Jul;67 ...
Reardon, JE (1989). "Herpes simplex virus type 1 and human DNA polymerase interactions with 2'-deoxyguanosine 5'-triphosphate ...
This DNA damage includes the oxidized nucleoside 8-oxo-2'-deoxyguanosine (8-oxo-dG), single- and double-strand breaks, DNA- ... January 1998). "DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine". ...
... deoxyguanosine levels after PAH exposure". Journal of Occupational and Environmental Medicine. 53 (7): 812-9. doi:10.1097/JOM. ...
Kaneko T, Tahara S, Matsuo M (May 1996). "Non-linear accumulation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidized DNA ...
Oxidative damage to DNA during aging: 8-hydroxy-2'-deoxyguanosine in rat organ DNA and urine. Proc Natl Acad Sci U S A 1990;87( ... 1998) DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proc Natl Acad ... 1998) DNA oxidation matters: the HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proc Natl Acad ... deoxyguanosine DNA lesions in mammalian cells. Carcinogenesis 20(12):2287-2292. PMID 10590221 Hamilton ML, Guo Z, Fuller CD, ...
Homologous types of purine riboswitches bind deoxyguanosine, but have more significant differences than a single nucleotide ...
The DGUOK gene encodes for mitochondrial deoxyguanosine kinase (dGK), which catalyzes the phosphorylation of ... "Molecular insight into mitochondrial DNA depletion syndrome in two patients with novel mutations in the deoxyguanosine kinase ... "Abnormal neurological features predict poor survival and should preclude liver transplantation in patients with deoxyguanosine ...
examined the fate of 8-oxo-dG when this oxidized derivative of deoxyguanosine was inserted into a specific gene in 800 cells in ... OGG1 is the primary enzyme responsible for the excision of 8-oxo-2'-deoxyguanosine (8-oxo-dG). Even when OGG1 expression is ...
Kakuma, T.; Nishida, J.; Tsuzuki, T.; Sekiguchi, M. (1995). "Mouse MTH1 protein with 8-oxo-7,8-dihydro-2′-deoxyguanosine 5′- ...
... deoxyguanosine (8-oxodG) concentration in peripheral blood lymphocytes is an index of nuclear DNA damage. 8-oxodG damages are ...
Pischetsrieder, Monika; Seidel, Wolfgang; Münch, Gerald; Schinzel, Reinhard (1999). "N2-(1-Carboxyethyl)deoxyguanosine, a ...
... reacts with deoxyadenosine and deoxyguanosine in DNA, forming DNA adducts, the primary one being M1G, which is ...
... deoxyguanosine analog with potent and selective anti-hepatitis B virus activity in vitro". Bioorganic & Medicinal Chemistry ...
The DNA is composed of four nucleotides (deoxyadenosine, deoxycytidine, deoxythymidine and deoxyguanosine), to the exclusion of ...
Deoxyguanosine (dG) nucleoside molecule. DNA (deoxyribonucleic acid) building block. Atoms are represented as spheres with ... Caption: Deoxyguanosine (dG) nucleoside molecule. DNA (deoxyribonucleic acid) building block. Atoms are represented as spheres ... deoxyguanosine, deoxyribonucleic, deoxyribonucleic acid, deoxyribose, dg, digitally generated, dna, formula, guanine, guanosine ...
... deoxyguanosine (oxo8dG) is a mutagenic oxidized base formed in DNA during the metabolism of B[a]P by the peroxidase and ... The lung has been shown to be a target organ for the effects of Benzo[a]pyrene (B[a]P). 8-hydroxy-2-deoxyguanosine (oxo8dG) is ... Selective accumulation of 8-hydroxy-2-deoxyguanosine in the lung following acute treatment with benzo(a)pyrene.. ...
This modification is available as part of a custom oligonucleotide configuration. Please use OLIGObuilder™ to generate pricing and order or contact us to speak with a customer service representative. ...
In order to estimate the level of oxidative damage and its role in breast cancer, 8-hydroxy-2-deoxyguanosine (8-OHdG) was ... In order to estimate the level of oxidative damage and its role in breast cancer, 8-hydroxy-2-deoxyguanosine (8-OHdG) was ... Increased formation of oxidative DNA damage, 8-hydroxy-2-deoxyguanosine, in human breast cancer tissue and its relationship to ...
8-Hydroxy-2′-deoxyguanosine (8-OH-dG), which has been regarded as a potential marker of oxidative DNA damage induced by ... Shigenaga M. K., Gimeno C. J., Ames B. N. Urinary 8-hydroxy-2′-deoxyguanosine as a biological marker of in vivo oxidative DNA ... Shigenaga M. K., Aboujaoude E. N., Chen Q., Ames B. N. Assays of oxidative DNA damage biomarkers 8-oxo-2′-deoxyguanosine and 8- ... Kasai H. Analysis of a form of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, as a marker of cellular oxidative stress ...
Altered p50 binding affinity and repair shielding by 7,8-dihydro-8-oxo-2′-deoxyguanosine lesions in the NF-κB promoter element ... Cellular background level of 8-oxo-7,8-dihydro-2′-deoxyguanosine: an isotope based method to evaluate artefactual oxidation of ... Base excision repair of reactive oxygen species-initiated 7,8-dihydro-8-oxo-2′-deoxyguanosine inhibits the cytotoxicity of ... Base excision repair of reactive oxygen species-initiated 7,8-dihydro-8-oxo-2′-deoxyguanosine inhibits the cytotoxicity of ...
Base excision repair of reactive oxygen species-initiated 7,8-dihydro-8-oxo-2′-deoxyguanosine inhibits the cytotoxicity of ... Base excision repair of reactive oxygen species-initiated 7,8-dihydro-8-oxo-2′-deoxyguanosine inhibits the cytotoxicity of ... Base excision repair of reactive oxygen species-initiated 7,8-dihydro-8-oxo-2′-deoxyguanosine inhibits the cytotoxicity of ... Base excision repair of reactive oxygen species-initiated 7,8-dihydro-8-oxo-2′-deoxyguanosine inhibits the cytotoxicity of ...
Derivatives of 2 -Deoxyguanosine. 4 Results Catalog #. Product Name. CAS. Structure. Y-1131. N2-Isobutyryl-2-deoxyguanosine. ... N2-Isobutyryl-3-O-(4,4-dimethoxytrityl)-2-deoxyguanosine. Y-1137. N2-Isobutyryl)-5-O-[(N,N-diisopropylamino)-(2-cyanoethoxy ... N2-Isobutyryl-5-O-tert-butyldimethylsilyl-2-deoxyguanosine. Y-1135. ...
Toward consensus in the analysis of urinary 8-oxo-7,8-dihydro-2′- deoxyguanosine as a noninvasive biomarker of oxidative stress ... deoxyguanosine as a noninvasive biomarker of oxidative stress. FASEB Journal 24 (4) : 1249-1260. [email protected] Repository. ...
In nuclear and mitochondrial DNA, 8-hydroxy-2 -deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2 -deoxyguanosine (8-oxodG) is ... In nuclear and mitochondrial DNA, 8-hydroxy-2 -deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2 -deoxyguanosine (8-oxodG) is ... Effect of a single bout of exercise and beta-carotene supplementation on the urinary excretion of 8-hydroxy-deoxyguanosine in ... 8-hydroxy-2 -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis.. ...
Deoxyguanosine Catalog Number: 07-4000 CAS Number: 961-07-9 Molecular Formula: 285.20 Molecular Weight: C10H13N5O4 H2O ...
Global Deoxyguanosine Market by Manufacturers, Countries, Type and Application, Forecast to 2022 published in Aug 2017 spread ... Deoxyguanosine Anhydrate. *Deoxyguanosine Monohydrate. Market Segment by Applications, can be divided into*Intermediate. * ... Chapter 2, to analyze the top manufacturers of Deoxyguanosine, with sales, revenue, and price of Deoxyguanosine, in 2016 and ... There are 15 Chapters to deeply display the global Deoxyguanosine market.. Chapter 1, to describe Deoxyguanosine Introduction, ...
You are viewing an interactive 3D depiction of the molecule 8-(4-amino-4-biphenylyl)-2-deoxyguanosine (C22H23N6O4) from the PQR.
This entry was posted in Antioxidants, Cell Membrane, Inflammation and tagged 8-Oxo-2-deoxyguanosine, 8-oxo-dG, antioxidants, ... Oxidative DNA Damage Assessed by Concentrations of 8-Oxo-2′-deoxyguanosine in the Cell. ... When DNA is impacted by oxidative stress, the chemical 8-Oxo-2′-deoxyguanosine (8-oxo-dG) is produced as a byproduct. ...
found in: NADP Disodium Salt Trihydrate, DNTP Set, 2-Deoxyguanosine Monohydrate, Adenosine-5-Triphosphate (ATP), Uracil, DATP ... 2-Deoxyguanosine Monohydrate Amresco. Grade: Ultra Pure Molecular Weight: 285.26. Compare this item. ...
Regional Industry Coverage from 2020 to 2027 Summary of the Report Growth of the Deoxyguanosine market ... Deoxyguanosine Global Market Deoxyguanosine Market Deoxyguanosine Market Analysis Deoxyguanosine Market 2021 " Deoxyguanosine ... Market Growth Deoxyguanosine Market Price Deoxyguanosine Market Research Deoxyguanosine Market Scope Deoxyguanosine Market ... Segmentation and Scope of the Deoxyguanosine Market. The Deoxyguanosine market is bi-furcated into different segments based on ...
The nuclear 8-OHdG levels were high (more than two lesions per 106 deoxyguanosines) from 7 days to 104 weeks of age in the ... 8-Hydroxy-2′-deoxyguanosine in rat organ DNA and urine. Proc Natl Acad Sci USA 87:4533-4537. ... The HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine. Proc Natl Acad Sci USA 95:288-293. ... The nuclear 8-OHdG levels were high (more than two lesions per 106 deoxyguanosines) from 7 days to 104 weeks of age in the ...
... it becomes deoxyguanosine monophosphate. 8-Oxo-2-deoxyguanosine "2-deoxyguanosine". PubChem Compound Database. National ... Deoxyguanosine is composed of the purine nucleobase guanine linked by its N9 nitrogen to the C1 carbon of deoxyribose. It is ...
Deoxyguanosine monophosphate (dGMP), also known as deoxyguanylic acid or deoxyguanylate in its conjugate acid and conjugate ...
Deoxyguanosine kinase deficiency is an inherited disorder that can cause liver disease and neurological problems. Explore ... Deoxyguanosine kinase deficiency is inherited in an autosomal recessive pattern. , which means both copies of the gene in each ... Deoxyguanosine Kinase Deficiency. 2009 Jun 18 [updated 2016 Dec 22]. In: Adam MP, Ardinger HH, Pagon RA, Wallace SE, Bean LJH, ... The DGUOK gene provides instructions for making the enzyme deoxyguanosine kinase. This enzyme plays a critical role in ...
The two forms of deoxyguanosine kinase (DGUOK) deficiency are a neonatal multisystem disorder and an isolated hepatic disorder ... Normal gene product. Deoxyguanosine kinase (DGUOK) is a mitochondrial protein that has a molecular weight of 28 kd with 277 ... Recessive deoxyguanosine kinase deficiency causes juvenile onset mitochondrial myopathy. Mol Genet Metab. 2012;107:92-4. [ ... Deoxyguanosine Kinase Deficiency. 2009 Jun 18 [Updated 2016 Dec 22]. In: Adam MP, Ardinger HH, Pagon RA, et al., editors. ...
Buy our Recombinant Human Deoxyguanosine kinase protein. Ab158280 is a full length protein produced in Wheat germ and has been ... Recombinant Human Deoxyguanosine kinase protein. See all Deoxyguanosine kinase proteins and peptides. ... Mitochondrial deoxyguanosine kinase (DGUOK) is required for the phosphorylation of several deoxyribonucleosides and certain ... and mitochondrial deoxyguanosine kinase (DGUOK also known as DGK). DGUOK expression is ubiquitous, with highest levels in ...
2-deoxyguanosine-5-triphosphate-dgtp-trisodium-salt-aqueous-purine-nucleoside-triphosphate-ab146559.pdf ... 2-Deoxyguanosine 5-triphosphate [dGTP] trisodium salt (aqueous), purine nucleoside triphosphate (ab146559). ...
Phosphodiester Group Attached to Deoxyguanosine does not Accelerate the Hydrolysis of cis-. [. PtCl. (. NH. 3. ). 2. (. dGuo. ) ...
Protein target information for Deoxyguanosine kinase (Staphylococcus aureus). Find diseases associated with this biological ...
Oxidative damage to DNA during aging: 8-hydroxy-2-deoxyguanosine in rat organ DNA and urine.. C G Fraga, M K Shigenaga, J W ... Steady-state levels of oh8dG ranged from 8 to 73 residues per 10(6) deoxyguanosine residues or 0.2-2.0 x 10(5) residues per ... The oxidized nucleoside, 8-hydroxy-2-deoxyguanosine (oh8dG), one of the approximately 20 known oxidative DNA damage products, ... Oxidative damage to DNA during aging: 8-hydroxy-2-deoxyguanosine in rat organ DNA and urine. ...
8-Hydroxy-2-deoxyguanosine (8-OHdG), a marker of oxidative stress, has been recently rediscovered to inhibit Rac1 in ... 8-Hydroxy-2-deoxyguanosine prevents plaque formation and inhibits vascular smooth muscle cell activation through Rac1 ... 8-Hydroxy-2-deoxyguanosine prevents plaque formation and inhibits vascular smooth muscle cell activation through Rac1 ... 8-Hydroxy-2-deoxyguanosine prevents plaque formation and inhibits vascular smooth muscle cell activation through Rac1 ...
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
... deoxyguanosine 5-monophosphate; which is mediated by the enzyme deoxyguanosine kinase. In humans, deoxyguanosine is involved ... Deoxyguanosine can be converted to 8-hydroxy-deoxyguanosine (8-OHdG) due to hydroxyl radical attack at the C8 of guanine. It is ... Deoxyguanosine. Description. Deoxyguanosine, also known as dG, belongs to the class of organic compounds known as purine 2- ... Deoxyguanosine is an extremely weak basic (essentially neutral) compound (based on its pKa). Deoxyguanosine exists in all ...
Deoxyguanosine. Description. Deoxyguanosine is a nucleoside consisting of the base guanine and the sugar deoxyribose. It is ... Deoxyguanosine can be converted to 8-hydroxy-deoxyguanosine (8-OHdG) due to hydroxyl radical attack at the C8 of guanine. 8- ... Showing metabocard for Deoxyguanosine (HMDB0000085). Jump To Section: IdentificationTaxonomyOntologyPhysical propertiesSpectra ... 6. Deoxyguanosine kinase, mitochondrial. General function:. Involved in ATP binding. Specific function:. Required for the ...
2′-Deoxyguanosine methylation at the N7 position (MdG) is a major mechanism of action of some DNA alkylating agents. We ... The predominant consequence of N7-methyl-2′-deoxyguanosine (MdG) is widely believed to be abasic site (AP) formation via ... Histone tails decrease N7-methyl-2′-deoxyguanosine depurination and yield DNA-protein cross-links in nucleosome core particles ... Histone tails decrease N7-methyl-2′-deoxyguanosine depurination and yield DNA-protein cross-links in nucleosome core particles ...
... deoxyguanosine 3′-O-succinic acid for your research needs. Find product specific information including CAS, MSDS, protocols and ... N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine 3′-O-succinic acid ~95% ... I6508 - N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine 3′-O-succinic acid ...
Among the oxidative damages, base modifications, such as oxidation of deoxyguanosine to 8-hydroxy-2′-deoxyguanosine (8-OHdG) ... At 8 weeks after the onset of diabetes, levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is a marker of oxidative DNA ... Fraga CG, Shigenaga MK, Park JW, Degan P, Ames BN: Oxidative damage to DNA during aging: 8-hydroxy-2′-deoxyguanosine in rat ... Shigenaga MK, Gimeno CJ, Ames BN: Urinary 8-hydroxy-2′-deoxyguanosine as a biological marker of in vivo oxidative DNA damage. ...
2-DEOXYGUANOSINE-5-MONOPHOSPHATE. C10 H14 N5 O7 P. LTFMZDNNPPEQNG-KVQBGUIXSA-N. Ligand Interaction. ... A new crystal form of bovine pancreatic RNase A in complex with 2-deoxyguanosine-5-monophosphate.. Larson, S.B., Day, J.S., ... A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2-Deoxyguanosine-5-monophosphate. *DOI: 10.2210/pdb2QCA/pdb ... The structure of bovine pancreatic RNase A has been determined in complex with 2-deoxyguanosine-5-monophosphate (dGMP) at ...
8-Hydroxy-deoxyguanosine. Description. 8-hydroxy-deoxyguanosine (8-OHdG) is a sensitive marker of the DNA damage due to ... Tarng DC, Huang TP, Wei YH, Liu TY, Chen HW, Wen Chen T, Yang WC: 8-hydroxy-2-deoxyguanosine of leukocyte DNA as a marker of ... Tarng DC, Huang TP, Liu TY, Chen HW, Sung YJ, Wei YH: Effect of vitamin E-bonded membrane on the 8-hydroxy 2-deoxyguanosine ... Sabatini L, Barbieri A, Tosi M, Roda A, Violante FS: A method for routine quantitation of urinary 8-hydroxy-2-deoxyguanosine ...
Deoxyguanosine monohydrate (cas 142340-99-6) prices, manufacturers, suppliers, exporters start with Guidechem!Global mass 2- ... Name: 2-Deoxyguanosine monohydrate. *Synonyms: CA0117; AN-6719; Preveon; HY-B0255; DR003071; TC-070886; LS-173141; youheding; ... There are 423 suppliers who can provide 2-Deoxyguanosine monohydrate with CAS No. 142340-99-6 ...
... deoxyguanosine by a perimidinone-derived synthetic nucleoside: a DNA interstrand stacking interaction. ... Structure of O6-Benzyl-2-deoxyguanosine opposite perimidinone-derived synthetic nucleoside in DNA duplex. ...
... deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same ... Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-78-dihydro-2-deoxyguanosine (8-oxo-dG) as a Possible Tumor ... "Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-78-dihydro-2-deoxyguanosine (8-oxo-dG) as a Possible Tumor ... deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same ...
... deoxyguanosine ELISA kit is a sandwich enzyme immunoassay for the quantitative measurement of 8-hydroxy-2-deoxyguanosine. ( ... 8-hydroxy-2-deoxyguanosine ELISA kit is a sandwich enzyme immunoassay for the quantitative measurement of 8-hydroxy-2- ... Oxidative damage to DNA during aging: 8-hydroxy-2-deoxyguanosine in rat organ DNA and urine.. Fraga CG, Shigenaga MK, Park JW ...
Abstract 10269: Urinary 8-hydroxy-2′-deoxyguanosine as a Novel Biomarker of Inflammatory Activity in Patients with Cardiac ... Abstract 10269: Urinary 8-hydroxy-2′-deoxyguanosine as a Novel Biomarker of Inflammatory Activity in Patients with Cardiac ... Abstract 10269: Urinary 8-hydroxy-2′-deoxyguanosine as a Novel Biomarker of Inflammatory Activity in Patients with Cardiac ... Abstract 10269: Urinary 8-hydroxy-2′-deoxyguanosine as a Novel Biomarker of Inflammatory Activity in Patients with Cardiac ...
... Fyrberg, Anna ... Deoxyguanosine kinase, melanoma, leukemia, nucleoside analog, RNAi HSV kategori Identifikatorer. URN: urn:nbn:se:liu:diva-64066 ... and deoxyguanosine kinase (dGK) and some of the deactivating enzymes called 5nucleotidases (5-NTs). We have measured mRNA ...
  • Effect of a single bout of exercise and beta-carotene supplementation on the urinary excretion of 8-hydroxy-deoxyguanosine in humans. (scienceopen.com)
  • 8-hydroxy-2' -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. (scienceopen.com)
  • In nuclear and mitochondrial DNA, 8-hydroxy-2' -deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2' -deoxyguanosine (8-oxodG) is one of the predominant forms of free radical-induced oxidative lesions, and has therefore been widely used as a biomarker for oxidative stress and carcinogenesis. (scienceopen.com)
  • When DNA is impacted by oxidative stress, the chemical 8-Oxo-2′-deoxyguanosine (8-oxo-dG) is produced as a byproduct. (biofoundations.org)
  • In order to estimate the level of oxidative damage and its role in breast cancer, 8-hydroxy-2'-deoxyguanosine (8-OHdG) was determined in DNA isolated from human breast tissue. (nih.gov)
  • Temporal variation of hydroxyl radical generation and 8-hydroxy-2'-deoxyguanosine formation by coarse and fine particulate matter. (scienceopen.com)
  • The Deoxyguanosine market is bi-furcated into different segments based on their type, component, application and geography. (mccourier.com)
  • The nuclear 8-OHdG levels were high (more than two lesions per 10 6 deoxyguanosines) from 7 days to 104 weeks of age in the brain, 3 to 6 weeks in the adrenal gland, 6 to 104 weeks in the lung, and 3 to 52 weeks in the testis. (nature.com)
  • Selective accumulation of 8-hydroxy-2'-deoxyguanosine in the lung following acute treatment with benzo(a)pyrene. (cdc.gov)
  • Growth of the Deoxyguanosine market is due to the increasing consumer's awareness, high demand and ongoing research and development. (mccourier.com)
  • The oxidized nucleoside, 8-hydroxy-2'-deoxyguanosine (oh8dG), one of the approximately 20 known oxidative DNA damage products, has been measured in DNA isolated from various organs of Fischer 344 rats of different ages. (pnas.org)
  • Deoxyguanosine is a nucleoside consisting of the base guanine and the sugar deoxyribose. (hmdb.ca)
  • In humans, deoxyguanosine is involved in the metabolic disorder called the purine nucleoside phosphorylase deficiency pathway. (hmdb.ca)
  • Deoxyguanosine is found to be associated with purine nucleoside phosphorylase (PNP) deficiency, which is an inborn error of metabolism. (hmdb.ca)
  • We have used human leukemic cell lines, and isolated cells from patients with leukemia, to investigate the nucleoside analog activating enzymes deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK) and some of the deactivating enzymes called 5'nucleotidases (5'-NTs). (diva-portal.org)
  • Here, we report that the nucleoside analogue 6-thio-2′-deoxyguanosine (6-thio-dG) is recognized by telomerase and is incorporated into de novo -synthesized telomeres. (aacrjournals.org)
  • Cytosolic nucleoside phosphorylase (NP) trimer catalyzes the reversible reaction of guanosine or deoxyguanosine with orthophosphate to form guanine and ribose 1-phosphate or deoxyribose 1-phosphate (Ealick et al. (reactome.org)
  • Here, we investigated if cell membrane transporters are involved in the cellular uptake of the nucleoside analogue DNA damage mediator 6-thio-2′-deoxyguanosine (6-thio-dG). (elsevier.com)
  • The DGUOK gene provides instructions for making the enzyme deoxyguanosine kinase. (medlineplus.gov)
  • Mutations in the DGUOK gene reduce or eliminate the activity of the deoxyguanosine kinase enzyme. (medlineplus.gov)
  • The two forms of deoxyguanosine kinase (DGUOK) deficiency are a neonatal multisystem disorder and an isolated hepatic disorder that presents later in infancy or childhood. (nih.gov)
  • The diagnosis of deoxyguanosine kinase (DGUOK) deficiency ( DGUOK -related hepatocerebral mitochondrial DNA depletion syndrome) should be suspected in individuals with a combination of the following clinical, supportive laboratory, and liver biopsy findings. (nih.gov)
  • Mitochondrial deoxyguanosine kinase (DGUOK) is required for the phosphorylation of several deoxyribonucleosides and certain purine deoxykribonucleoside analogs widely employed as antiviral and chemotherapeutic agents. (abcam.com)
  • In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by 2 deoxyribonucleoside kinases: cytosolic deoxycytidine kinase (DCK) and mitochondrial deoxyguanosine kinase (DGUOK also known as DGK). (abcam.com)
  • A, p.E227K) in the deoxyguanosine gene ( DGUOK ). (bmj.com)
  • The mitochondrial deoxynucleotide triphosphate (dNTP) is maintained by the mitochondrial deoxynucleoside salvage pathway and dedicated for the mtDNA homeostasis, and the mitochondrial deoxyguanosine kinase (DGUOK) is a rate-limiting enzyme in this pathway. (unmc.edu)
  • Both drugs form platinum-DNA cross-linked adducts, and cisplatin causes oxidative DNA damage including the 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxodG) lesion. (aacrjournals.org)
  • structure see text] After bioactivation, the potent dietary mutagen 2-amino-3-methylimidazo[4,5-f]-quinoline (IQ) reacts with DNA to give two regioisomeric adducts of deoxyguanosine. (scienceexchange.com)
  • These intermediates, including ROS, induce lipid peroxidation, and DNA damage including DNA strand breakage, oxidation to 8-oxo-2'-deoxyguanosine, and DNA-adducts. (nih.gov)
  • Deoxyguanosine kinase deficiency is an inherited disorder that can cause liver disease and neurological problems. (medlineplus.gov)
  • Newborns with the hepatocerebral form of deoxyguanosine kinase deficiency may have a buildup of lactic acid in the body (lactic acidosis) within the first few days after birth. (medlineplus.gov)
  • Some individuals with deoxyguanosine kinase deficiency have a milder form of the disorder without severe neurological problems. (medlineplus.gov)
  • The prevalence of deoxyguanosine kinase deficiency is unknown. (medlineplus.gov)
  • These problems lead to the neurological and liver dysfunction associated with deoxyguanosine kinase deficiency. (medlineplus.gov)
  • Dimmock DP, Dunn JK, Feigenbaum A, Rupar A, Horvath R, Freisinger P, Mousson de Camaret B, Wong LJ, Scaglia F. Abnormal neurological features predict poor survival and should preclude liver transplantation in patients with deoxyguanosine kinase deficiency. (medlineplus.gov)
  • Moreover, TCDD (0.1nM) increased the levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), typical biomarker of oxidative stress, with time dependency in HepG2 cell, but just transiently increased the levels of 8-OHdG after 2 hours in WRL68 cell. (aacrjournals.org)
  • 8-hydroxy-2' -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. (scienceopen.com)
  • This study has analysed the generation of 3-(2-deoxy-β-D-erythro- pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine adduct [M1dG], a biomarker of oxidative stress and lipid peroxidation, in breast fine-needle aspirate samples of 22 patients with breast cancer, at different clinical stages, in respect to 13 controls. (elsevier.com)
  • Beginning with 3-cyclopenten-1-ylamine hydrochloride, the 5'-nor derivatives of carbocyclic 2'-deoxyguanosine (2), 2'-deoxyadenosine (3), and 2,6-diaminopurine 2'-deoxyribofuranoside (4) have been prepared. (kuleuven.be)
  • Deoxyguanosine exists in all living species, ranging from bacteria to humans. (hmdb.ca)
  • Within humans, deoxyguanosine participates in a number of enzymatic reactions. (hmdb.ca)
  • Effect of a single bout of exercise and beta-carotene supplementation on the urinary excretion of 8-hydroxy-deoxyguanosine in humans. (scienceopen.com)
  • Oxidative damage to DNA during aging: 8-hydroxy-2'-deoxyguanosine in rat organ DNA and urine. (pnas.org)
  • At 8 weeks after the onset of diabetes, levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG), which is a marker of oxidative DNA damage, were significantly increased in mtDNA from kidney of diabetic rats but not in nuclear DNA, suggesting the predominant damage of mtDNA. (diabetesjournals.org)
  • 8-hydroxy-deoxyguanosine (8-OHdG) is a sensitive marker of the DNA damage due to hydroxyl radical attack at the C8 of guanine. (hmdb.ca)
  • In order to estimate the level of oxidative damage and its role in breast cancer, 8-hydroxy-2'-deoxyguanosine (8-OHdG) was determined in DNA isolated from human breast tissue. (nih.gov)
  • 8-Hydroxy-2′-deoxyguanosine (8-OH-dG), which has been regarded as a potential marker of oxidative DNA damage induced by reactive oxygen species, was measured in human urine by a commercial ELISA using a monoclonal antibody N45.1 and by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector (HPLC-ECD) to evaluate whether the ELISA system is applicable to human monitoring studies. (aacrjournals.org)
  • We investigated whether urinary(U) 8-hydroxy-2′-deoxyguanosine (8-OHdG), a marker of oxidative DNA damage, correlates with inflammatory activity in SAR. (ahajournals.org)
  • The rate of oxidative DNA damage was estimated from 24 h urinary excretion of 8-oxo-2'-deoxyguanosine (8-oxodG), a repair product of the highly mutagenic oxidation of guanine in DNA or the cellular pool of GTP. (ku.dk)
  • There was an increase in parameters of DNA (8-oxo-7,8-dihydro-2'- deoxyguanosine ) and lipid (15-F2t- isoprostanes levels) oxidative damage in CBS-deficient patients when compared to controls. (bvsalud.org)
  • Significance: These findings identify a biomarker of resistance to the telomeric DNA damage mediator 6-thio-2′-deoxyguanosine. (elsevier.com)
  • Deoxyguanosine is composed of the purine nucleobase guanine linked by its N9 nitrogen to the C1 carbon of deoxyribose. (wikipedia.org)
  • Deoxyguanosine, also known as dG, belongs to the class of organic compounds known as purine 2'-deoxyribonucleosides. (hmdb.ca)
  • Synthesis of the N2-deoxyguanosine adduct of the potent dietary mutagen IQ. (scienceexchange.com)
  • If a phosphate group is attached at the 5' position, it becomes deoxyguanosine monophosphate. (wikipedia.org)
  • Deoxyguanosine monophosphate (dGMP), also known as deoxyguanylic acid or deoxyguanylate in its conjugate acid and conjugate base forms, respectively, is a derivative of the common nucleic acid guanosine triphosphate (GTP), in which the -OH (hydroxyl) group on the 2' carbon on the nucleotide's pentose has been reduced to just a hydrogen atom (hence the "deoxy-" part of the name). (wikipedia.org)
  • The structure of bovine pancreatic RNase A has been determined in complex with 2'-deoxyguanosine-5'-monophosphate (dGMP) at 1.33 A resolution at room temperature in a previously unreported unit cell belonging to space group P3(1). (rcsb.org)
  • which is mediated by the enzyme deoxyguanosine kinase. (hmdb.ca)
  • 8-hydroxy-2'-deoxyguanosine ELISA kit is a sandwich enzyme immunoassay for the quantitative measurement of 8-hydroxy-2'-deoxyguanosine. (abnova.com)
  • when cytosine arabinoside or deoxyguanosine was used as the substrate, the kinase activity in the extracts from the dGuo-R cells was only 10% of the enzyme activity in the extracts from the wild-type cells. (ecu.edu)
  • The role of deficiency of oxoguanine glycosylase 1 ( Ogg1 ) Mmh homolog, a repair enzyme of the 8-hydroxy-2'-deoxyguanosine (8-OHdG) residue in DNA, was investigated using the multiorgan carcinogenesis bioassay in mice. (mdpi.com)
  • Induction and excretion of UV-induced 8-oxo-2'-deoxyguanosine and thymine dimers in vivo: implications for PUVA. (dmu.ac.uk)
  • 8-Hydroxy-2'-deoxyguanosine (8OHdG) conjugated keyhole limpet hemocyanin. (adipogen.com)
  • 2′-Deoxyguanosine methylation at the N7 position (MdG) is a major mechanism of action of some DNA alkylating agents. (pnas.org)
  • The relationship between 8-oxo-7,8-dihydro-2'-deoxyguanosine level and extent of cytosine methylation in leukocytes DNA of healthy subjects and in patients with colon adenomas and carcinomas. (openrepository.com)
  • Among the oxidative damages, base modifications, such as oxidation of deoxyguanosine to 8-hydroxy-2′-deoxyguanosine (8-OHdG) and subsequent mutations of mitochondrial DNA (mtDNA), have received increasing attention in recent years. (diabetesjournals.org)
  • With baseline data collected from 2004 to 2008, the authors examined whether urinary 8-hydroxy-2-deoxyguanosine (8-OHdG), a biomarker of global DNA oxidation, was associated with cognitive function in a sample of 1,003 Puerto Rican adults, aged 45-75 years, living in Boston, Massachusetts, and the surrounding area. (semanticscholar.org)
  • radicals was triggered by the competitive oneelectron oxidation of dimethyl sulfoxide (DMSO) and 2'-deoxyguanosine (dG) by photochemically generated sulfate radicals in deoxygenated aqueous buffer solutions (pH 7.5). (elsevier.com)
  • This study aimed to elucidate the relationship of serum BDNF , malondialdehyde (MDA), and 8-Hydroxy 2- Deoxyguanosine (8-OhdG) levels in acute stroke cases with one-month post- stroke depression . (bvsalud.org)
  • Temporal variation of hydroxyl radical generation and 8-hydroxy-2'-deoxyguanosine formation by coarse and fine particulate matter. (scienceopen.com)
  • Thus, the manipulation of the interferon system by immunomodulatory compounds, such as deoxycytidyl-deoxyguanosine oligonucleotides (CpG ODN), has potential utility as a therapeutic intervention aimed at the prevention and control of viral infections ( 36 ). (asm.org)
  • These biological properties may be due to the inability of 2-4 to be phosphorylated to the requisite nucleotide level that is likely to be necessary for biological activity by correlation to carbocyclic 2'-deoxyguanosine (1), which possesses significant antiviral properties as a result of conversion to its 5'-triphosphate derivative. (kuleuven.be)
  • Solutions of nitrosated glycine reacted with 2'-deoxyguanosine and calf thymus DNA to give O6-carboxymethyl-2'-deoxyguanosine and, at high concentrations of glycine and nitric oxide, O6-methyl-2'-deoxyguanosine. (open.ac.uk)
  • To determine if deoxycytidyl-deoxyguanosine oligonucleotides (CpG ODN) can be used effectively as nonspecific inducers of innate immune defenses for preventative or therapeutic interventions in infectious disease models for nonhuman primates, the present study evaluated the response of rhesus monkey peripheral blood mononuclear cells to three different synthetic CpG ODN classes by defining the cytokine gene expression patterns and by characterizing IFN-α/β responses. (asm.org)
  • Gentaur Molecular :Gene Link \ O6 methyl deoxyguanosine 1 umol scale. (antibody-antibodies.com)
  • Detection of acetaldehyde derived N(2)-ethyl-2'-deoxyguanosine in human leukocyte DNA following alcohol consumption. (openrepository.com)
  • Selective accumulation of 8-hydroxy-2'-deoxyguanosine in the lung following acute treatment with benzo(a)pyrene. (cdc.gov)
  • 1980). While NP is active with either nuckeotide in vitro, levels of deoxyguanosine are normally low in vivo, limiting the extent of this reaction. (reactome.org)
  • Monofunctional alkylating agents preferentially react at the N7 position of 2′-deoxyguanosine in duplex DNA. (pnas.org)
  • Steady-state levels of oh8dG ranged from 8 to 73 residues per 10(6) deoxyguanosine residues or 0.2-2.0 x 10(5) residues per cell. (pnas.org)
  • The effect of alcohol consumption on the levels and persistence of N(2)-ethylidene-2'-deoxyguanosine (N(2)-ethylidene-dG) formed by acetaldehyde, the oxidative metabolite of ethanol, in human leukocyte DNA was investigated. (openrepository.com)
  • The levels of N(2)-ethyl-2'-deoxyguanosine (N(2)-ethyl-dG) in the DNA were determined following reduction of N(2)-ethylidene-dG with sodium cyanoborohydride using a liquid chromatography-tandem mass spectrometry selected reaction monitoring method. (openrepository.com)
  • Since the menstrual cycle may confound or modify this association, depressive symptoms and urinary levels of 8-hydroxy-2' deoxyguanosine (8-OHdG) were evaluated during each menstrual phase. (fujita-hu.ac.jp)
  • This study aimed to investigate the effect of high intensity interval training on 8-oxoguanine DNA glycosylase (OGG1) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in the brain and liver of rats. (who.int)
  • These include the (5′R) and (5′S) diastereomers of 8,5′-cyclo-2′-deoxyadenosine (cdA) and 8,5′-cyclo-2′- deoxyguanosine (cdG), which were also found to be present at appreciable levels in DNA isolated from mammalian cells and tissues. (umn.edu)
  • A mouse leukemia L1210 cell line was selected for resistance to deoxyguanosine. (ecu.edu)
  • Cory, Ann H. and Shibley, Ivan A. and Chalovich, Joseph M. and Cory, Joseph G., "Deoxyguanosine-resistant Leukemia L1210 Cells: Loss of Specific Deoxyribonecleoside Kinase Activity," Journal of Biological Chemistry 268, no. 1 (January 1993), http://hdl.handle.net/10342/3245 (accessed September 20, 2018). (ecu.edu)
  • Therefore, the aim of the present study was to assess a possible link between 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) background level and 5-methylcytosine content in DNA from human leukocytes of healthy subjects (n=105) as well as in patients with colon adenomas (n=39) and carcinomas (n=50). (openrepository.com)
  • IMSEAR at SEARO: Effect of high intensity interval training on 8-oxoguanine DNA glycosylase and 8-hydroxy-2'-deoxyguanosine contents in the brain and liver of rats. (who.int)
  • Ravanat JL, Cadet J, Araki K, Toma HE, Medeiros MHG de, Di Mascio P. Supramolecular cationic tetraruthenated porphyrin and light-induced decomposition of 2'-deoxyguanosine predominantly via a singlet oxygen-mediated mechanism. (usp.br)