The hard portion of the tooth surrounding the pulp, covered by enamel on the crown and cementum on the root, which is harder and denser than bone but softer than enamel, and is thus readily abraded when left unprotected. (From Jablonski, Dictionary of Dentistry, 1992)
The property of dentin that permits passage of light, heat, cold, and chemical substances. It does not include penetration by microorganisms.
Substances which reduce or eliminate dentinal sensitivity or the pain associated with a source of stimulus (such as touch, heat, or cold) at the orifice of exposed dentinal tubules causing the movement of tubular fluid that in turn stimulates tooth nerve receptors.
Cements that act through infiltration and polymerization within the dentinal matrix and are used for dental restoration. They can be adhesive resins themselves, adhesion-promoting monomers, or polymerization initiators that act in concert with other agents to form a dentin-bonding system.
Dentin formed by normal pulp after completion of root end formation.
An adhesion procedure for orthodontic attachments, such as plastic DENTAL CROWNS. This process usually includes the application of an adhesive material (DENTAL CEMENTS) and letting it harden in-place by light or chemical curing.
An apparently hereditary disorder of dentin formation, marked by a normal appearance of coronal dentin associated with pulpal obliteration, faulty root formation, and a tendency for peripheral lesions without obvious cause. (From Dorland, 27th ed)
Acrylic acids or acrylates which are substituted in the C-2 position with a methyl group.
Dental cements composed either of polymethyl methacrylate or dimethacrylate, produced by mixing an acrylic monomer liquid with acrylic polymers and mineral fillers. The cement is insoluble in water and is thus resistant to fluids in the mouth, but is also irritating to the dental pulp. It is used chiefly as a luting agent for fabricated and temporary restorations. (Jablonski's Dictionary of Dentistry, 1992, p159)
Preparation of TOOTH surfaces and DENTAL MATERIALS with etching agents, usually phosphoric acid, to roughen the surface to increase adhesion or osteointegration.
The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility.
Adherent debris produced when cutting the enamel or dentin in cavity preparation. It is about 1 micron thick and its composition reflects the underlying dentin, although different quantities and qualities of smear layer can be produced by the various instrumentation techniques. Its function is presumed to be protective, as it lowers dentin permeability. However, it masks the underlying dentin and interferes with attempts to bond dental material to the dentin.
Synthetic resins, containing an inert filler, that are widely used in dentistry.
The maximum stress a material subjected to a stretching load can withstand without tearing. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed, p2001)
The formation of dentin. Dentin first appears in the layer between the ameloblasts and odontoblasts and becomes calcified immediately. Formation progresses from the tip of the papilla over its slope to form a calcified cap becoming thicker by the apposition of new layers pulpward. A layer of uncalcified dentin intervenes between the calcified tissue and the odontoblast and its processes. (From Jablonski, Dictionary of Dentistry, 1992)
The susceptibility of the DENTIN to dissolution.
The process whereby calcium salts are deposited in the dental enamel. The process is normal in the development of bones and teeth. (Boucher's Clinical Dental Terminology, 4th ed, p43)
The description and measurement of the various factors that produce physical stress upon dental restorations, prostheses, or appliances, materials associated with them, or the natural oral structures.
Inorganic derivatives of phosphoric acid (H3PO4). Note that organic derivatives of phosphoric acids are listed under ORGANOPHOSPHATES.
A hard thin translucent layer of calcified substance which envelops and protects the dentin of the crown of the tooth. It is the hardest substance in the body and is almost entirely composed of calcium salts. Under the microscope, it is composed of thin rods (enamel prisms) held together by cementing substance, and surrounded by an enamel sheath. (From Jablonski, Dictionary of Dentistry, 1992, p286)
A tooth's loss of minerals, such as calcium in hydroxyapatite from the tooth matrix, caused by acidic exposure. An example of the occurrence of demineralization is in the formation of dental caries.
The reaction product of bisphenol A and glycidyl methacrylate that undergoes polymerization when exposed to ultraviolet light or mixed with a catalyst. It is used as a bond implant material and as the resin component of dental sealants and composite restorative materials.
A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)
An inner coating, as of varnish or other protective substance, to cover the dental cavity wall. It is usually a resinous film-forming agent dissolved in a volatile solvent, or a suspension of calcium hydroxide in a solution of a synthetic resin. The lining seals the dentinal tubules and protects the pulp before a restoration is inserted. (Jablonski, Illustrated Dictionary of Dentistry, 1982)
One of a set of bone-like structures in the mouth used for biting and chewing.
The part of a tooth from the neck to the apex, embedded in the alveolar process and covered with cementum. A root may be single or divided into several branches, usually identified by their relative position, e.g., lingual root or buccal root. Single-rooted teeth include mandibular first and second premolars and the maxillary second premolar teeth. The maxillary first premolar has two roots in most cases. Maxillary molars have three roots. (Jablonski, Dictionary of Dentistry, 1992, p690)
The most posterior teeth on either side of the jaw, totaling eight in the deciduous dentition (2 on each side, upper and lower), and usually 12 in the permanent dentition (three on each side, upper and lower). They are grinding teeth, having large crowns and broad chewing surfaces. (Jablonski, Dictionary of Dentistry, 1992, p821)
An operation in which carious material is removed from teeth and biomechanically correct forms are established in the teeth to receive and retain restorations. A constant requirement is provision for prevention of failure of the restoration through recurrence of decay or inadequate resistance to applied stresses. (Boucher's Clinical Dental Terminology, 4th ed, p239-40)
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
The lymph or fluid of dentin. It is a transudate of extracellular fluid, mainly cytoplasm of odontoblastic processes, from the dental pulp via the dentinal tubules. It is also called dental lymph. (From Stedman, 26th ed, p665)
Substances used to bond COMPOSITE RESINS to DENTAL ENAMEL and DENTIN. These bonding or luting agents are used in restorative dentistry, ROOT CANAL THERAPY; PROSTHODONTICS; and ORTHODONTICS.
Therapeutic technique for replacement of minerals in partially decalcified teeth.
Materials used in the production of dental bases, restorations, impressions, prostheses, etc.
The seepage of fluids, debris, and micro-organisms between the walls of a prepared dental cavity and the restoration.
The mechanical property of material that determines its resistance to force. HARDNESS TESTS measure this property.
The degree of approximation or fit of filling material or dental prosthetic to the tooth surface. A close marginal adaptation and seal at the interface is important for successful dental restorations.
A property of the surface of an object that makes it stick to another surface.
Characteristics or attributes of the outer boundaries of objects, including molecules.
Removal of minerals from bones during bone examination.
Localized destruction of the tooth surface initiated by decalcification of the enamel followed by enzymatic lysis of organic structures and leading to cavity formation. If left unchecked, the cavity may penetrate the enamel and dentin and reach the pulp.
Substances that cause the adherence of two surfaces. They include glues (properly collagen-derived adhesives), mucilages, sticky pastes, gums, resins, or latex.
Any of the eight frontal teeth (four maxillary and four mandibular) having a sharp incisal edge for cutting food and a single root, which occurs in man both as a deciduous and a permanent tooth. (Jablonski, Dictionary of Dentistry, 1992, p820)
Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.
The wearing away of a tooth as a result of tooth-to-tooth contact, as in mastication, occurring only on the occlusal, incisal, and proximal surfaces. It is chiefly associated with aging. It is differentiated from TOOTH ABRASION (the pathologic wearing away of the tooth substance by friction, as brushing, bruxism, clenching, and other mechanical causes) and from TOOTH EROSION (the loss of substance caused by chemical action without bacterial action). (Jablonski, Dictionary of Dentistry, 1992, p86)
Poly-2-methylpropenoic acids. Used in the manufacture of methacrylate resins and plastics in the form of pellets and granules, as absorbent for biological materials and as filters; also as biological membranes and as hydrogens. Synonyms: methylacrylate polymer; poly(methylacrylate); acrylic acid methyl ester polymer.
The teeth of the first dentition, which are shed and replaced by the permanent teeth.
The internal resistance of a material to moving some parts of it parallel to a fixed plane, in contrast to stretching (TENSILE STRENGTH) or compression (COMPRESSIVE STRENGTH). Ionic crystals are brittle because, when subjected to shear, ions of the same charge are brought next to each other, which causes repulsion.
A restoration designed to remain in service for not less than 20 to 30 years, usually made of gold casting, cohesive gold, or amalgam. (Jablonski, Dictionary of Dentistry, 1992)
Production of a radiographic image of a small or very thin object on fine-grained photographic film under conditions which permit subsequent microscopic examination or enlargement of the radiograph at linear magnifications of up to several hundred and with a resolution approaching the resolving power of the photographic emulsion (about 1000 lines per millimeter).
The space in a tooth bounded by the dentin and containing the dental pulp. The portion of the cavity within the crown of the tooth is the pulp chamber; the portion within the root is the pulp canal or root canal.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
The result of pathological changes in the hard tissue of a tooth caused by carious lesions, mechanical factors, or trauma, which render the pulp susceptible to bacterial invasion from the external environment.
The quality or state of being wettable or the degree to which something can be wet. This is also the ability of any solid surface to be wetted when in contact with a liquid whose surface tension is reduced so that the liquid spreads over the surface of the solid.
An autosomal dominant disorder of tooth development characterized by opalescent dentin resulting in discoloration of the teeth. The dentin develops poorly with low mineral content while the pulp canal is obliterated.
The constricted part of the tooth at the junction of the crown and root or roots. It is often referred to as the cementoenamel junction (CEJ), the line at which the cementum covering the root of a tooth and the enamel of the tooth meet. (Jablonski, Dictionary of Dentistry, 1992, p530, p433)
The aftermost permanent tooth on each side in the maxilla and mandible.
The upper part of the tooth, which joins the lower part of the tooth (TOOTH ROOT) at the cervix (TOOTH CERVIX) at a line called the cementoenamel junction. The entire surface of the crown is covered with enamel which is thicker at the extremity and becomes progressively thinner toward the cervix. (From Jablonski, Dictionary of Dentistry, 1992, p216)
It is used as an oxidizing and bleaching agent and as a disinfectant. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Inability or inadequacy of a dental restoration or prosthesis to perform as expected.
Dentifrices that are formulated into a paste form. They typically contain abrasives, HUMECTANTS; DETERGENTS; FLAVORING AGENTS; and CARIOSTATIC AGENTS.
Progressive loss of the hard substance of a tooth by chemical processes that do not involve bacterial action. (Jablonski, Dictionary of Dentistry, 1992, p296)
Chemicals used mainly to disinfect root canals after pulpectomy and before obturation. The major ones are camphorated monochlorophenol, EDTA, formocresol, hydrogen peroxide, metacresylacetate, and sodium hypochlorite. Root canal irrigants include also rinsing solutions of distilled water, sodium chloride, etc.
Application of a protective agent to an exposed pulp (direct capping) or the remaining thin layer of dentin over a nearly exposed pulp (indirect capping) in order to allow the pulp to recover and maintain its normal vitality and function.
The bonelike rigid connective tissue covering the root of a tooth from the cementoenamel junction to the apex and lining the apex of the root canal, also assisting in tooth support by serving as attachment structures for the periodontal ligament. (Jablonski, Dictionary of Dentistry, 1992)
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The pathologic wearing away of the tooth substance by brushing, bruxism, clenching, and other mechanical causes. It is differentiated from TOOTH ATTRITION in that this type of wearing away is the result of tooth-to-tooth contact, as in mastication, occurring only on the occlusal, incisal, and proximal surfaces. It differs also from TOOTH EROSION, the progressive loss of the hard substance of a tooth by chemical processes not involving bacterial action. (From Jablonski, Dictionary of Dentistry, 1992, p2)
One of the protein CROSS-LINKING REAGENTS that is used as a disinfectant for sterilization of heat-sensitive equipment and as a laboratory reagent, especially as a fixative.
Preparation of TOOTH surfaces, and of materials bonded to teeth or DENTAL IMPLANTS, with agents and methods which roughen the surface to facilitate adhesion. Agents include phosphoric or other acids (ACID ETCHING, DENTAL) and methods include LASERS.
A solution used for irrigating the mouth in xerostomia and as a substitute for saliva.
A disinfectant and topical anti-infective agent used also as mouthwash to prevent oral plaque.
The process of TOOTH formation. It is divided into several stages including: the dental lamina stage, the bud stage, the cap stage, and the bell stage. Odontogenesis includes the production of tooth enamel (AMELOGENESIS), dentin (DENTINOGENESIS), and dental cementum (CEMENTOGENESIS).
Erbium. An element of the rare earth family of metals. It has the atomic symbol Er, atomic number 68, and atomic weight 167.26.
A polymer obtained by reacting polyacrylic acid with a special anion-leachable glass (alumino-silicate). The resulting cement is more durable and tougher than others in that the materials comprising the polymer backbone do not leach out.
A test to determine the relative hardness of a metal, mineral, or other material according to one of several scales, such as Brinell, Mohs, Rockwell, Vickers, or Shore. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Break or rupture of a tooth or tooth root.
Procedures carried out with regard to the teeth or tooth structures preparatory to specified dental therapeutic and surgical measures.
Preparatory activities in ROOT CANAL THERAPY by partial or complete extirpation of diseased pulp, cleaning and sterilization of the empty canal, enlarging and shaping the canal to receive the sealing material. The cavity may be prepared by mechanical, sonic, chemical, or other means. (From Dorland, 28th ed, p1700)
The hardening or polymerization of bonding agents (DENTAL CEMENTS) via exposure to light.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
Substances that inhibit or arrest DENTAL CARIES formation. (Boucher's Clinical Dental Terminology, 4th ed)
Inflammation of the DENTAL PULP, usually due to bacterial infection in dental caries, tooth fracture, or other conditions causing exposure of the pulp to bacterial invasion. Chemical irritants, thermal factors, hyperemic changes, and other factors may also cause pulpitis.
Polymeric resins derived from OXIRANES and characterized by strength and thermosetting properties. Epoxy resins are often used as dental materials.
Cylindrical epithelial cells in the innermost layer of the ENAMEL ORGAN. Their functions include contribution to the development of the dentinoenamel junction by the deposition of a layer of the matrix, thus producing the foundation for the prisms (the structural units of the DENTAL ENAMEL), and production of the matrix for the enamel prisms and interprismatic substance. (From Jablonski's Dictionary of Dentistry, 1992)
A group of phosphate minerals that includes ten mineral species and has the general formula X5(YO4)3Z, where X is usually calcium or lead, Y is phosphorus or arsenic, and Z is chlorine, fluorine, or OH-. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Use of a metal casting, usually with a post in the pulp or root canal, designed to support and retain an artificial crown.
Mesodermal tissue enclosed in the invaginated portion of the epithelial enamel organ and giving rise to the dentin and pulp.
Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.
Dental caries involving the tooth root, cementum, or cervical area of the tooth.
Loss of the tooth substance by chemical or mechanical processes
The act of cleaning teeth with a brush to remove plaque and prevent tooth decay. (From Webster, 3d ed)
The collective tissues from which an entire tooth is formed, including the DENTAL SAC; ENAMEL ORGAN; and DENTAL PAPILLA. (From Jablonski, Dictionary of Dentistry, 1992)
A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable.
Exudate from seeds of the grape plant Vitis vinifera, composed of oils and secondary plant metabolites (BIOFLAVONOIDS and polyphenols) credited with important medicinal properties.
A highly glycosylated and sulfated phosphoprotein that is found almost exclusively in mineralized connective tissues. It is an extracellular matrix protein that binds to hydroxyapatite through polyglutamic acid sequences and mediates cell attachment through an RGD sequence.
Inorganic salts of hydrofluoric acid, HF, in which the fluorine atom is in the -1 oxidation state. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Sodium and stannous salts are commonly used in dentifrices.
Materials placed inside a root canal for the purpose of obturating or sealing it. The materials may be gutta-percha, silver cones, paste mixtures, or other substances. (Dorland, 28th ed, p631 & Boucher's Clinical Dental Terminology, 4th ed, p187)
A prosthesis or restoration placed for a limited period, from several days to several months, which is designed to seal the tooth and maintain its position until a permanent restoration (DENTAL RESTORATION, PERMANENT) will replace it. (From Jablonski, Dictionary of Dentistry, 1992)
Inorganic compounds that contain carbon as an integral part of the molecule but are not derived from hydrocarbons.
Inorganic compounds that contain calcium as an integral part of the molecule.
The generic term for salts derived from silica or the silicic acids. They contain silicon, oxygen, and one or more metals, and may contain hydrogen. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th Ed)
The tip or terminal end of the root of a tooth. (Jablonski, Dictionary of Dentistry, 1992, p62)
Any preparations used for cleansing teeth; they usually contain an abrasive, detergent, binder and flavoring agent and may exist in the form of liquid, paste or powder; may also contain medicaments and caries preventives.
Incomplete fracture of any part of a tooth, characterized by pain during mastication and sensitivity to heat, cold, sweet or sour tastes, and alcohol; it is often undiagnosed because the tooth is usually X-ray negative and normal to pulp vitality tests.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chemical reaction in which monomeric components are combined to form POLYMERS (e.g., POLYMETHYLMETHACRYLATE).

The root surface in human teeth: a microradiographic study. (1/1113)

In an attempt to clarify the nature of the human cemento-dentinal junction, ground sections of incompletely formed and fully formed extracted teeth were prepared and their histology compared with their microradiographic appearances. The results showed that incompletely formed teeth possess distinctive surface layers outside the granular layer of Tomes. The evidence indicates that these layers are of dentinal origin; their presence during development supports previous explanations by the author of the hyaline layer of Hopewell-Smith and of so-called intermediate cementum. The results also indicate that the granular layer of Tomes does not represent the outer limit of root dentine. The relationship of these surface layers to the definitive cementum which is present in fully formed teeth was studied in both young and older patients. From the results it was concluded that cementum formation begins in the more apical region of the teeth at a time when root formation is well advanced, and that it spreads towards the crown rather than in the generally accepted reverse direction.  (+info)

Predicting protein decomposition: the case of aspartic-acid racemization kinetics. (2/1113)

The increase in proportion of the non-biological (D-) isomer of aspartic acid (Asp) relative to the L-isomer has been widely used in archaeology and geochemistry as a tool for dating. the method has proved controversial, particularly when used for bones. The non-linear kinetics of Asp racemization have prompted a number of suggestions as to the underlying mechanism(s) and have led to the use of mathematical transformations which linearize the increase in D-Asp with respect to time. Using one example, a suggestion that the initial rapid phase of Asp racemization is due to a contribution from asparagine (Asn), we demonstrate how a simple model of the degradation and racemization of Asn can be used to predict the observed kinetics. A more complex model of peptide bound Asx (Asn + Asp) racemization, which occurs via the formation of a cyclic succinimide (Asu), can be used to correctly predict Asx racemization kinetics in proteins at high temperatures (95-140 degrees C). The model fails to predict racemization kinetics in dentine collagen at 37 degrees C. The reason for this is that Asu formation is highly conformation dependent and is predicted to occur extremely slowly in triple helical collagen. As conformation strongly influences the rate of Asu formation and hence Asx racemization, the use of extrapolation from high temperatures to estimate racemization kinetics of Asx in proteins below their denaturation temperature is called into question. In the case of archaeological bone, we argue that the D:L ratio of Asx reflects the proportion of non-helical to helical collagen, overlain by the effects of leaching of more soluble (and conformationally unconstrained) peptides. Thus, racemization kinetics in bone are potentially unpredictable, and the proposed use of Asx racemization to estimate the extent of DNA depurination in archaeological bones is challenged.  (+info)

Steric effects of N-acyl group in O-methacryloyl-N-acyl tyrosines on the adhesiveness of unetched human dentin. (3/1113)

We have prepared various O-methacryloyl-N-acyl tyrosines (MAATY) to reveal the relationship between molecular structure near carboxylic acid and adhesive strength of MAATY-HEMA type adhesive resin to unetched dentin. In this study, we attempted to change the steric hindrance effect without changing the HLB value, i.e., introducing an iso-acyl group instead of n-acyl group into MAATY. O-methacryloyl-N-ethylbutyryl tyrosine (MIHTY) showed significantly lower adhesive strength when compared with O-methacryloyl-N-hexanoyl tyrosine even though both MAATY have the same HLB value. The possible explanation of the significantly different adhesive strength was that the 2-ethylbutyryl group in MIHTY was bulky, resulting in inhibition of the hydrogen bonding of the carboxylic group. The HLB value is independent of the steric effect of molecular structure, and thus the steric factor should be taken into consideration for the explanation of different adhesive strengths within the adhesive monomers having the same HLB value but different molecular structures.  (+info)

Dentinal tubule occlusion with lanthanum fluoride and powdered apatite glass ceramics in vitro. (4/1113)

To simulate hypersensitive dentin, the smear layer and dentinal plugs of bovine root dentin specimens were removed by immersion in 10% phosphoric acid, polishing with hydroxyapatite particles, and ultrasonic cleansing. The fluoride-tannic acid-lanthanum-apatite (FTLA) group was treated with acidulated phosphate fluoride (APF) containing tannic acid followed by rubbing with a paste of lanthanum chloride (LaCl3) and powdered apatite glass ceramics. The treated specimens were immersed in a remineralizing solution that mimics saliva for 6 weeks. The SEM observations revealed that the treated surfaces of the FTLA group were completely covered with fine spherical compounds and the dentinal tubules were occluded with plugs to a depth of about 3 microns. Fluoride and lanthanum were detected to a depth of over 20 microns by EPMA observation. After the remineralization, the surface of FTLA-treated specimen did not have any opened tubules and showed a remarkable increase in the number of fine spherical deposits in the dentinal tubules. These results suggest that the reaction products produced by sequential treatment with acidic fluoride and LaCl3 and powdered apatite glass ceramics are able to effectively occlude dentinal tubules.  (+info)

Osteoclast differentiation factor acts as a multifunctional regulator in murine osteoclast differentiation and function. (5/1113)

Osteoclast differentiation factor (ODF), a novel member of the TNF ligand family, is expressed as a membrane-associated protein by osteoblasts/stromal cells. The soluble form of ODF (sODF) induces the differentiation of osteoclast precursors into osteoclasts in the presence of M-CSF. Here, the effects of sODF on the survival, multinucleation, and pit-forming activity of murine osteoclasts were examined in comparison with those of M-CSF and IL-1. Osteoclast-like cells (OCLs) formed in cocultures of murine osteoblasts and bone marrow cells expressed mRNA of RANK (receptor activator of NF-kappaB), a receptor of ODF. The survival of OCLs was enhanced by the addition of each of sODF, M-CSF, and IL-1. sODF, as well as IL-1, activated NF-kappaB and c-Jun N-terminal protein kinase (JNK) in OCLs. Like M-CSF and IL-1, sODF stimulated the survival and multinucleation of prefusion osteoclasts (pOCs) isolated from the coculture. When pOCs were cultured on dentine slices, resorption pits were formed on the slices in the presence of either sODF or IL-1 but not in that of M-CSF. A soluble form of RANK as well as osteoprotegerin/osteoclastogenesis inhibitory factor, a decoy receptor of ODF, blocked OCL formation and prevented the survival, multinucleation, and pit-forming activity of pOCs induced by sODF. These results suggest that ODF regulates not only osteoclast differentiation but also osteoclast function in mice through the receptor RANK.  (+info)

Deficiency of SHP-1 protein-tyrosine phosphatase activity results in heightened osteoclast function and decreased bone density. (6/1113)

Mice homozygous for the motheaten (Hcphme) or viable motheaten (Hcphme-v) mutations are deficient in functional SHP-1 protein-tyrosine phosphatase and show severe defects in hematopoiesis. Comparison of femurs from mev/mev mice revealed significant decreases in bone mineral density (0.33 +/- 0.03 mg/mm3 for mev/mevversus 0.41 +/- 0.01 mg/mm3 for controls) and mineral content (1.97 +/- 0.36 mg for mev/mevversus 10.64 +/- 0.67 for controls) compared with littermate controls. Viable motheaten mice also showed reduced amounts of trabecular bone and decreased cortical thickness. These bone abnormalities were associated with a 14% increase in numbers of multinucleated osteoclasts and an increase in osteoclast resorption activity. In co-cultures of normal osteoblasts with mutant or control bone marrow cells, numbers of osteoclasts developing from mutant mice were increased compared with littermate control mice. Although mev/mev osteoclasts develop in the absence of colony-stimulating factor (CSF)-1, nevertheless cultured osteoclasts show increased size in the presence of CSF-1. CSF-1-deficient osteopetrosis (op/op) mutant mice develop severe osteosclerosis. However, doubly homozygous mev/mevop/op mice show an expansion of bone marrow cavities and reduced trabecular bone mass compared with op/op mice. Western blot analysis showed that several proteins that were markedly hyperphosphorylated on tyrosine residues were detected in the motheaten osteoclasts, including a novel 126-kd phosphotyrosine protein. The marked hyperphosphorylation of a 126-kd protein in motheaten osteoclasts suggests that this protein depends on SHP-1 for dephosphorylation. These findings demonstrate that the decreased SHP-1 catalytic activity in me/me and mev/mev mice results in an increased population of activated osteoclasts and consequent reduction in bone density.  (+info)

Intermittent inhibition of dentin mineralization of rat incisors under continual infusion of 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) using a subcutaneous mini osmotic pump. (7/1113)

The inhibitory effect of the continual administration of 1-hydroxyethylidene-1, 1-bisphosphonate (HEBP) (8 mgP/kg/day) through a mini osmotic pump on dentin mineralization was examined in relation to the diurnal rhythm of the rat and compared with that of daily injections of same amounts of HEBP known to inhibit dentin mineralization. After daily injections of HEBP, a series of alternating rows of mineralized and non-mineralized dentin islands appeared in the newly formed portion of the crown-analogue of rat incisors. A similar phenomenon occurred under the continual administration of HEBP in rats raised either under regular environmental photofraction or constant lighting conditions. The average distance between the adjacent mineralized dentin islands was 521.0 +/- 51.3 microns in the injected rats. After continual HEBP administration, this was 426.0 +/- 13.2 microns and 416.5 +/- 19.4 microns under ordinary photofraction and constant light, respectively. Although the pattern of individual mineralized dentin islands tended to become irregular in nocturnal rats, no statistical difference was noted between the two values. Rows of mineralized and non-mineralized dentin islands also appeared in the root analogue dentin. No sign of the intermittent inhibition of mineralization was recognized in mesodermal hard tissues other than dentin in the HEBP-affected animals. These data implicate the presence of intrinsic cycles in dentin mineralization at the growing end of rat incisors independent of environmental photofraction as well as the ameloblast function.  (+info)

Interaction between vacuolar H(+)-ATPase and microfilaments during osteoclast activation. (8/1113)

Vacuolar H(+)-ATPases (V-ATPases) are multisubunit enzymes that acidify compartments of the vacuolar system of all eukaryotic cells. In osteoclasts, the cells that degrade bone, V-ATPases, are recruited from intracellular membrane compartments to the ruffled membrane, a specialized domain of the plasma membrane, where they are maintained at high densities, serving to acidify the resorption bay at the osteoclast attachment site on bone (Blair, H. C., Teitelbaum, S. L., Ghiselli, R., and Gluck, S. L. (1989) Science 249, 855-857). Here, we describe a new mechanism involved in controlling the activity of the bone-resorptive cell. V-ATPase in osteoclasts cultured in vitro was found to form a detergent-insoluble complex with actin and myosin II through direct binding of V-ATPase to actin filaments. Plating bone marrow cells onto dentine slices, a physiologic stimulus that activates osteoclast resorption, produced a profound change in the association of the V-ATPase with actin, assayed by coimmunoprecipitation and immunocytochemical colocalization of actin filaments and V-ATPase in osteoclasts. Mouse marrow and bovine kidney V-ATPase bound rabbit muscle F-actin directly with a maximum stoichiometry of 1 mol of V-ATPase per 8 mol of F-actin and an apparent affinity of 0.05 microM. Electron microscopy of negatively stained samples confirmed the binding interaction. These findings link transport of V-ATPase to reorganization of the actin cytoskeleton during osteoclast activation.  (+info)

TY - JOUR. T1 - Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization in vitro. AU - Kim, Jongryul. AU - Vaughn, Ryan M.. AU - Gu, Lisha. AU - Rockman, Roy A.. AU - Arola, Dwayne D.. AU - Schafer, Tara E.. AU - Choi, Kyoung Kyu. AU - Pashley, David H.. AU - Tay, Franklin R.. PY - 2010/6/15. Y1 - 2010/6/15. N2 - Degradation of hybrid layers created in primary dentin occurs as early as 6 months in vivo. Biomimetic remineralization utilizes bottom-up nanotechnology principles for interfibrillar and intrafibrillar remineralization of collagen matrices. This study examined whether imperfect hybrid layers created in primary dentin can be remineralized. Coronal dentin surfaces were prepared from extracted primary molars and bonded using Adper Prompt L-Pop and a composite. One-millimeter-thick specimen slabs of the resin-dentin interface were immersed in a Portland cement-based remineralization medium that contained ...
TY - JOUR. T1 - Site specific properties of carious dentin matrices biomodified with collagen cross-linkers. AU - Bedran-Russo, Ana K.. AU - Karol, Sachin. AU - Pashley, David H.. AU - Viana, Grace. N1 - Copyright: Copyright 2013 Elsevier B.V., All rights reserved.. PY - 2013/10. Y1 - 2013/10. N2 - Purpose: To assess in non-cavitated carious teeth the mechanical properties of dentin matrix by measuring its reduced modulus of elasticity and the effect of dentin biomodification strategies on three dentin matrix zones: caries-affected, apparently normal dentin below caries-affected zone and sound dentin far from carious site. Methods: Nano-indentations were performed on dentin matrices of carious molars before and after surface modification using known cross-linking agents (glutaraldehyde, proanthocyanidins from grape seed extract and carbodiimide). Results: Statistically significant differences were observed between dentin zones of demineralized dentin prior to surface biomodification (P, 0.05). ...
This study was conducted to compare the shear bond strengths of composite resin bonded to Er:YAG laser or bur-prepared dentin surfaces using three self-etching adhesive systems. The occlusal surfaces of 120 human third molars were ground flat to expose dentin. The dentin was prepared using either a carbide bur or an Er:YAG laser at 350 mJ/pulse and 10 Hz (fluence, 44.5 J/cm2). Three different self-etching adhesive systems were applied: iBond™, Xeno III™ and Clearfil SE Bond™. Rods of composite resin were bonded to dentin surfaces and shear bond tests were carried out. Both dentin surfaces after debonding and resin rods were observed using a scanning electron microscope. When the Xeno III™ was used, no difference was observed on shear bond strength values when bur and Er:YAG laser were compared. When using iBond™ and Clearfil SE Bond™, bond strength values measured on Er:YAG-laser-prepared surfaces were lower than those observed on bur-prepared surfaces. The absence of smear layer formation
TY - JOUR. T1 - Comparative post irrigation evaluation of calcium loss and its effect on microhardness of radicular dentin. AU - Khosla, Manak. AU - Mala, Kundabala. AU - Shenoy, Ramya. PY - 2017/10/1. Y1 - 2017/10/1. N2 - This study was conducted to compare calcium loss and microhardness reduction of radicular dentin following treatment with 17% EDTA, 10% citric acid, 5% maleic acid, and MTAD, by estimating calcium loss, and radicular dentin microhardness by using Atomic absorption spectrophotometry and by Vickers hardness tester. The results were statistically evaluated using one way ANOVA test and performed at 95% level of confidence and Spearmans correlation test was applied to determine correlation between calcium liberation and radicular dentin microhardness. All the experimental chelating agents bring about calcium loss and reduction in microhardness from radicular dentin.Study concluded that at 5 minutes, 5% maleic acid as a chelating agent causes the maximum calcium loss and ...
OBJECTIVES: The submicron hiatus represents a potential space between the base of the collagen network and the mineralized dentin when dentin is acid-etched for bonding. These spaces were observed in SEM studies after acid-etched dentin specimens were critical point dried or dehydrated in hexamethyldisilasane. However, they have never been identified in TEM studies of dentin hybrid layers. This study critically examined the cause of submicron hiati formation using a silver staining technique to measure nanoleakage. METHODS: Two multi-step, total-etch adhesives (One-Step, Bisco; Single Bond, 3M) and two single-step, self-etching adhesives (Prompt L-Pop, ESPE; One-Up Bond F, Tokuyama) were examined. Flat dentin surfaces were bonded with these adhesives and a lining composite. In each adhesive group, 0.8mm thick slabs from the same bonded tooth were coated with nail varnish applied 1mm from the bonded interfaces. The varnish was either left to dry completely for 10min before immersing in 50wt% ...
Purpose: To assess the effect of different peri- and intertubular dentin mineralization conditions and etching on shear bond strength in vitro. Materials and Methods: One hundred fifty crowns of extracted bovine incisors were embedded in resin and ground to expose the buccal coronal dentin. Sixty specimens were subjected to a demineralizing solution (DS) and another 60 teeth to a bacterial-based laboratory caries model (S. mutans, SM). Thirty specimens of each demineralization protocol (DS and SM) were randomly selected and remineralized (-R). Thirty sound dentin specimens served as control (C). Resin composite buildups (Tetric) were bonded after application of one of the following adhesives: a one-step self-etching adhesive (Xeno III), and a self-etching adhesive (Syntac Classic) without (three-step) and with prior additional 35% phosphoric acid etching (etch-and-rinse, four-step). Teeth were subjected to shear bond strength testing in a universal testing device at a crosshead speed of 0.5 ...
Introduction. The mechanism for adhesive bonding to dental substrates, especially to dentin, has been extensively studied in the last few decades. The establishment of effective interlocking occurs when the adhesive penetrates into the intratubular and intertubular dentin so that resin penetration into the conditioned dentin results in the formation of intratubular resin tags and a hybrid layer.1 Micromechanical attachment is the most important mechanism of resin adhesion to dentin. However, there are several factors that can affect hybrid layer and resin tag formation, such as acid-etching, the moisture conditions of the substrate, and adhesive and dentin depth.2. During dentin acid-etching, the mineral content of the dentin surface is removed, and the collagen fibrils remain supported by water.3 The moist bonding technique prevents the collagen fibers from collapsing and provides stability to the collagen, thus enabling greater infiltration of adhesive monomers.4 However, some studies have ...
Statement of the Problem: Low pH of self-etch adhesives might cause suboptimal polymerization. Purpose: This study aimed to evaluate the effect of dentin depth (deep and superficial) on polymerization efficacy of two self-etch adhesives, with different pH by means of Knoop hardness test. Materials and Method: In this in vitro study, sixty sound molars were used to prepare 30 superficial dentin and 30 deep dentin specimens. Dentin specimens of each depth were randomly distributed into two equal subgroups (N=15) and bonded by either Adper Prompt L-Pop (strong self-etch adhesive) or Adper Easy Bond (mild self-etch adhesive). Knoop hardness test was employed to evaluate degree of cross-linking of the adhesives. Data were analyzed with SPSS 16, using two-way ANOVA to compare mean hardness values of the study groups (p | 0.05). Results: There was no interaction effect between dentin depth and the type of adhesive (p = 0.36). Regardless of dentin depth, hardness of Adper Easy Bond was significantly higher than
TY - JOUR. T1 - The effect of flowable composite lining and dentin location on microtensile bond strength and internal fracture formation. AU - Kominami, Nao. AU - Shimada, Yasushi. AU - Hosaka, Keiichi. AU - Luong, Minh Nguyet. AU - Yoshiyama, Masahiro. AU - Sadr, Alireza. AU - Sumi, Yasunori. AU - Tagami, Junji. PY - 2019. Y1 - 2019. N2 - The objective of this study was to determine the effect of flowable composite lining and dentin location on internal dentin fracture formation in the microtensile bond strength (MTBS) test using swept-source optical coherence tomography (SS-OCT). MTBS test beams (1.0×1.0 mm) were prepared from human superficial and deep dentin, which was bonded with a self-etch adhesive (Clearfil SE Bond) and hybrid composite resin (Clearfil AP-X), with or without flowable lining (Clearfil Majesty ES-Flow). We tested 4 groups according to placement technique (with vs. without flowable liner) and dentin (superficial vs. deep) locations. Cross-sectional 2D images of the bonded ...
Microtensile bond strength of Resin composite to Normal dentin and Caries - affected dentin with total-etch and self-etch bonding adhesives before and after thermocycling
The influence of chlorhexidine on the remineralization of demineralized dentine Duck-Su Kim , Jongryul Kim , Kyoung-Kyu Choi and Sun-Young Kim Journal of Dentistry, 2011-12-01, Volume 39, Issue 12, Pages 855-862, Copyright © 2011 Elsevier Ltd Abstract Objectives To examine the differences in the amounts of bound chlorhexidine (CHX) on demineralized dentine blocks and to investigate the different aspects of remineralization of demineralized dentine according to different concentrations of CHX. Methods Dentine blocks (2 mm × 7 mm × 0.9 mm) were demineralized in 0.2 M formic acid solution. Amount of bound CHX on the dentine blocks was measured on a spectrophotometer after the dentine block was soaked in 0.02%, 0.2%, or 2% CHX solutions for 1 min. The change in elastic modulus of dentine block stored in simulated body fluids was measured at 0 (baseline), 2, 4, and 6 weeks after storage. The micromorphological aspects of the samples were observed using a field emission scanning electron microscope ...
Definition of primary dentin in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is primary dentin? Meaning of primary dentin as a legal term. What does primary dentin mean in law?
The aim of this study was to examine the morphology of the hybrid layer (HL) of bonded water-stored specimens after different chemical pre-treatments of dentin. Materials and methods. Twenty-seven recently extracted human molars were selected. Fifty-four dentin disks in middle/deep dentin were obtained with a slow speed saw with a diamond-impregnated disk under water lubrication. Smear layers were created with 180 grit silicon carbide under running water for 1 min. Different pre-treatments of dentin were: Group (1) no treatment; Group (2) 35% H3PO4 etch for 15 s followed by 10% glutaraldehyde for 120 s; Group (3) 37% H3PO4 etch for 15 s followed by 5% NaOCl for 120 s. Three dentin bonding agents (DBAs), Prime and Bond NT (P and B), AdmiraBond (AB), and Clearfil SE Bond (SEB) were applied in association with a resin composite following the manufacturers directions. Each specimen was then longitudinally sectioned and polished with wet SiC papers (up to #4000 grit). Impressions of the polished ...
The use of protein cross-linking agents during bonding procedures has been recently proposed to improve bond durability. This study aimed to use zymography and in situ zymography techniques to evaluate the ability of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) cross-linker to inhibit matrix metalloproteinase (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) EDC pre-treatment prevents this enzymatic activity. The zymographic assay was performed on protein extracts obtained from dentin powder treated with Optibond FL or Scotchbond 1XT with or without 0.3M EDC pre-treatment. For in situ zymography, adhesive/dentin interfaces were created with the same adhesives applied to acid-etched dentin slabs pre-treated or not with EDC conditioner. Zymograms revealed increased expression of dentin endogenous MMP-2 and -9 after adhesive application, while the use of EDC as a primer inactivated dentin gelatinases. Results of in situ zymograpy
TY - JOUR. T1 - Demonstration of type III collagen in the dentin of mice. AU - Nagata, Kengo. AU - Huang, Yu Hsin. AU - Ohsaki, Yasuyoshi. AU - Kukita, Toshio. AU - Nakata, Minoru. AU - Kurisu, Kojiro. PY - 1992/1/1. Y1 - 1992/1/1. N2 - It has been reported that, although type III collagen is present in human dentin where there is dentinogenesis imperfecta and in reparative dentin, it is absent in normal dentin. In a preliminary study, however, we observed evidence showing that small amounts of fibers showing positive labeling for type III collagen are present in the molars of normal mice. In the present study, in order to localize type III in normal dentin, immunofluorescent and immunoelectron microscopic examinations of the molars of normal mice were carried out using affinity-purified antibodies to mouse type III and type I collagen. The fibers positive for type III collagen were much more frequently observed in the root than in the crown. These fibers ran in peritubular dentin or near that ...
TY - JOUR. T1 - The effects of radicular dentine treated with double antibiotic paste and ethylenediaminetetraacetic acid on the attachment and proliferation of dental pulp stem cells. AU - Kim, Ki Wan. AU - Yassen, Ghaeth H.. AU - Ehrlich, Ygal. AU - Spolnik, Kenneth. AU - Platt, Jeffrey A.. AU - Windsor, L. Jack. PY - 2015/10/1. Y1 - 2015/10/1. N2 - This study explored the effects of dentine treated with two concentrations of double antibiotic paste (DAP) and ethylenediaminetetraacetic acid (EDTA) on the attachment and proliferation of dental pulp stem cells (DPSCs). Materials and Methods: Radicular dentine samples were prepared with identical dimensions and randomized into six groups (n = 4). Four groups were treated with double antibiotic paste (DAP) at concentrations of 500 mg ml-1 or 1 mg ml-1 with or without EDTA. The other two groups were treated with EDTA only or received no treatment. DPSCs were seeded on each dentine sample (10 000 cells per sample). Lactate dehydrogenase activity ...
In this study, a fluorescence technique was developed for visualization of dentin using confocal laser scanning microscopy (CLSM). Eighteen extracted human teeth were used: 13 showing no clinical signs of caries and 5 with visually apparent decay. Preliminary study: All teeth were horizontally sectioned to approx. 200 micrometers thickness and pre-treated as follows: no pretreatment; vacuum only; ultrasonication only; sodium hypochlorite (NaOCl) only; vacuum and NaOCl; ultrasonication and NaOCl; or vacuum, ultrasonication and NaOCl. Samples were stained with Rhodamine 123 fluorescent dye at a concentration of 10-5 M in phosphate buffer saline for 1 to 24 hours. Caries study: Dentin surfaces, some with pre-existing caries, were visualized using CLSM. Most dentin tubules in sound dentin appeared open using CLSM, but most dentin tubules in carious dentin appeared closed or narrowed. Surface images obtained using CLSM were similar to those seen by SEM, but additional subsurface imaging was possible ...
Introduction: Oxidant bleaching agents may induce several alterations on mineralized teeth tissues. Our aim is to study, at the ultrastructural level, mechanical modifications induced on dentin after exposure to different bleaching agents. Methods: Nanoindentation performed with atomic force microscopy was used to measure changes in dentin stiffness as well as the adhesion force between the tip and the tissue both in intertubular and peritubular dentin. For each specimen, dentin localization, and bleaching agent, 100 independent nanoindentations were performed. Carbamide peroxide (30%) and hydrogen peroxide (35%) were used as bleaching agents. Results: A significant reduction of both stiffness and adhesion force was found for both carbamide and hydrogen peroxide in peritubular and intertubular dentin. Conclusions: The use of bleaching agents led to a significant reduction in dentin local (at the nanoscale) mechanical properties. ...
Dentin is a calcified tissue of the body. Dentin, enamel, cementum and pulp, are the four major components that make up the teeth. It is often covered by enamel on the crown and cementum of the root and surrounds the tooths pulp. Dentin is comprised of 45% hydroxylapatite, 33% organic material and 22% water. Dentin is yellow in its appearance and greatly affects the tooths color because of the enamels translucency. Dentin is less mineralized and less brittle in comparison to enamel. It is also necessary for the support of the enamel. Dentin has a score of 3 on the Mohs scale of mineral hardness. Dentin forms throughout life and is very sensitive.. Dentinal sclerosis or transparent dentin-sclerosis of primary dentin is the regressive alteration in teeth caused by the calcification of the dentinal tubules. It can occur as a result of cavities, an abrasion or as part of the normal aging process. ...
The influence of dentin permeability on transdentinal LED light propagation should be evaluated since this kind of phototherapy may further be clinically used to stimulate the metabolism of pulp cells, improving the healing of damaged pulps. This study evaluated the influence of the dentin permeability on the transdentinal LED light (630 nm) transmission. Forty-five 0.5-mm-thick dentin disks were prepared from the coronal dentin of extracted sound human molars. An initial measurement of transdentinal LED light transmission was carried out by illuminating the discs in the occlusal-to-pulpal direction onto a light power sensor to determine light attenuation. The discs were treated with EDTA for smear layer removal, subjected to analysis of hydraulic conductance, and a new measurement of transdentinal LED light transmission was taken. Spearmans correlation coefficient was used for analysis of data and showed a weak correlation between dentin permeability and light attenuation (coefficient = 0.19). ...
Microtensile bond strength was evaluated in two separate experiments, performed in two different laboratories, following the same bonding procedures and using same materials. The teeth were sectioned under water cooling coronally to remove occlusal enamel and to expose flat dentin surface, and at the dentin-enamel junction. Exposed dentin surface was ground with 180-grit abrasive paper to create uniform smear layer, and the teeth were randomly divided into two groups. In the control specimens, the adhesives were used as recommended by the manufacturer. Briefly, the dentin surface was first acid-etched with 37% phosphoric acid for 15 s, rinsed, and gently dried with compressed air to achieve slightly dry surface. Then water was scrubbed into the surface with cotton pellet for 30 s and gently dried, leaving the surface slightly moist, and 1-step etch-and-rinse adhesive Scotchbond 1XT was used as per manufacturers recommendations, and polymerized with 20 s blue light irradiation (Elipar Freelight ...
Dentin hypersensitivity is a painful response to a non-noxious stimulus applied to exposed dentin. Two processes may expose dentin: loss of enamel and/or loss of cementum. Loss of enamel occurs by attrition associated with occlusal function, by abrasion from dietary components or incorrect toothbrushing, or by erosion associated with environmental or dietary components, particularly acids. Exposure of root dentin is also multifactorial. Periodontal disease with gingival recession, some forms of periodontal surgery, and overzealous brushing are important etiological factors that expose root dentin. In addition, in some individuals the cementum and enamel do not meet, exposing an area of dentin. The management of this condition requires a good understanding of the complexity of the problem as well as the variety of treatments available. Some authors report that lasers may provide reliable and reproducible treatment of dentin hypersensitivity. One concern for laser safety is that the heat produced ...
TY - JOUR. T1 - Influence of NaOCl deproteinization on shear bond strength in function of dentin depth. AU - Toledano, Manuel. AU - Perdigão, Jorge. AU - Osorio, Estrella. AU - Osorio, Raquel. PY - 2002/8/1. Y1 - 2002/8/1. N2 - Purpose: To determine the influence of NaOCl application on shear bond strengths (SBS) to superficial and deep dentin, using Prime & Bond 2.1. Materials and Methods: Superficial and deep dentin was exposed in 40 extracted third molars by sectioning the occlusal surface immediately under the enamel-dentin junction or close to the pulp chamber. After polishing the dentin disks (600-grit SiC), they were assigned to two groups: (1) 36% orthophosphoric acid for 15 seconds, or (2): 36% orthophosphoric acid for 15 seconds followed by 5% sodium hypochlorite (NaOCl) for 2 minutes. The dentin adhesive was applied as per manufacturers instructions, followed by TPH resin-based composite. The specimens were stored in water for 24 hours at 37°C and thermocycled x500 and SBS were ...
PURPOSE: To evaluate microtensile bond strength and interfacial nanoleakage expression of adhesive interfaces created by XP-Bond on human deproteinized dentin immediately after bonding and after 6 months of artificial aging. MATERIALS AND METHODS: Noncarious human molars were selected, middle/deep dentin substrates were exposed, and either assigned to group 1 (XP-Bond applied on collagen-depleted dentin) or to group 2 (XP-Bond applied in accordance with manufacturers instructions). In group 1, the etched dentin surface was treated with 10% NaOCl for 60 s to remove the exposed demineralized organic matrix before XP-Bond application. Composite/dentin beams were obtained in accordance with the microtensile nontrimming technique and either pulled to failure after 24 h or after 6 months artificial aging. Data were analyzed with two-way ANOVA and Tukeys post-hoc test (p , 0.05). Interfacial nanoleakage evaluation was performed on additional adhesive interfaces to quantify the amount of silver ...
Dentin (/ˈdɛntɪn/) (American English) or dentine (/ˈdɛnˌtiːn/ or /ˌdɛnˈtiːn/) (British English) (Latin: substantia eburnea) is a calcified tissue of the body and, along with enamel, cementum, and pulp, is one of the four major components of teeth. It is usually covered by enamel on the crown and cementum on the root and surrounds the entire pulp. By weight, 45% of dentin consists of the mineral hydroxylapatite, 33% is organic material, and 22% is water. Yellow in appearance, it greatly affects the color of a tooth due to the translucency of enamel. Dentin, which is less mineralized and less brittle than enamel, is necessary for the support of enamel. Dentin rates approximately 3 on the Mohs scale of mineral hardness. Dentinal sclerosis/transparent dentin-sclerosis of primary dentin is regressive alteration in tooth characterized by calcification of dentinal tubules. It can occur as a result of injury to dentin by caries or abrasion, or as part of the normal aging process. The ...
TY - JOUR. T1 - In vitro evaluation of dentin tubule occlusion for novel calcium lactate phosphate (CLP) paste. AU - Yang, Jen Chang. AU - Hu, Hsin Tai. AU - Lee, Sheng Yang. AU - Hsieh, Sung Chih. AU - Huang, Pei Chi. AU - Ma, Chen Feng. AU - Ji, Dian Yu. AU - Chang, Liang Yu. AU - Teng, Nai Chia. PY - 2017. Y1 - 2017. N2 - Introduction: The objective of this in vitro study is to evaluate the effective and long-term occlusion of dentinal tubules using a novel calcium lactate phosphate (CLP) based desensitizing agent. Methods: Dentin disks (n = 9) were pre-etched using 1 M lactic acid for 30 s and individually treated with Colgate® Pro-Relief™ paste, CLP paste, and double distilled water (ddH2O) by a rubber-cupped handpiece. Dentin disks were analyzed under optical micrographs for pre-treatment, directly after treatment, and 14 days post-treatment. One-way ANOVA and post-hoc Tukeys test were used to determine whether there were any statistically significant differences in dentinal tubule ...
PubMed journal article Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in viv were found in PRIME PubMed. Download Prime PubMed App to iPhone, iPad, or Android
The purpose of this study was to determine the uptake of fluoride by dentin after pulsed Nd:YAG laser irradiation. Under SEM examination dentin lased by pulsed Nd:YAG laser appeared to be melted; there were no changes of calcium and phosphorus levels. Dentin irradiated by pulsed Nd:YAG laser and treated with 38% Ag(NH3)2F showed greater uptake and deep penetration of fluoride than topical application of fluoride alone, SEM examination revealed that CaF2-like and Ag3PO4-like deposits were formed on the dentin surface. These results suggested that pulsed Nd:YAG laser irradition could improve the absorption of fluoride in dentin ...
Objectives. Contemporary adhesives lose their bond strength to dentin regardless of the bonding system used. This loss relates to the hydrolysis of collagen matrix of the hybrid layers. the preservation of the collagen matrix integrity is a key issue in the attempts to improve the dentin bonding durability.Methods. Dentin contains collagenolytic enzymes, matrix metalloproteinases (MMPs) and cysteine cathepsins, which are responsible for the hydrolytic degradation of collagen matrix in the bonded interface.Results. the identities, roles and function of collagenolytic enzymes in mineralized dentin has been gathered only within last 15 years, but they have already been demonstrated to have an important role in dental hard tissue pathologies, including the degradation of the hybrid layer. Identifying responsible enzymes facilitates the development of new, more efficient methods to improve the stability of dentin-adhesive bond and durability of bond strength.Significance. Understanding the nature and ...
Objectives: The purpose of this study was to evaluate the effects of load cycling on nanoleakage of occlusal flat surface or cervical restorations bonded with three self-etching priming systems and a single bottle system. Methods: The dentin bonding systems used in this study were Clearfil SE Bond, Unifil Bond, Prompt L-Pop and Single Bond. Flat occlusal dentin surfaces and cervical cavities from extracted human molars were bonded with one of the dentin bonding systems. After load cycling of 90N for 5000 cycles, samples were immersed in a 50% w/v solution of silver nitrate for 24h, and exposed to photodeveloping solution for 8h. Specimens were sectioned, mounted on stubs, carbon-coated and observed in a Field Emission-SEM using backscattered electron mode. One-way ANOVA was used for statistical analysis of the leakage scores. Results: FE-SEM images showed that samples subjected to load cycling had leakage patterns similar to non-load-cycled samples for all dentin bonding systems. Load cycling ...
Concentrations of calcium and phosphorus were measured in the neonatal dentine of 11 crabeater and 11 Weddell seal postcanine teeth with an energy-dispersive x-ray analyser. The extent of variation in elemental concentrations in different parts of the tooth, differences between species and individuals, and whether variation in elemental concentrations can provide information about dentine deposition mechanisms were assessed. No consistent patterns in elemental deposition in different parts of the tooth were found, but there were differences in concentrations between and within species. Post-natal dentine is composed of layers that appear alternately bright and dark in backscattered electron images. The elemental composition of neonatal dentine was closer to the dark bands than to those that appeared bright. It is suggested that the composition of neonatal dentine is more similar to the dark than the bright layers of dentine because of nutritional stresses that were occurring during mineral ...
Purpose: To examine the effects of collagenase and esterase activity on the microtensile bond strength and nanoleakage at the resin-dentin interfaces of two adhesive systems: a total-etch adhesive (Single Bond 2: SB) and a self-etch adhesive (Clearfil SE Bond: SE). Methods: Resin composites were bonded to the occlusal dentin surfaces of extracted human premolars with either SB or SE. The bonded teeth were sectioned into beams and assigned to one of four storage conditions: phosphate buffer solution (24 hours), phosphate buffer solution (4 weeks), collagenase solution (4 weeks), or esterase solution (4 weeks). Microtensile bond strength was evaluated and analyzed by two-way ANOVA. Failure mode was analyzed under SEM, and nanoleakage was examined with TEM. Results: The bond strength of SE was superior to that of SB after 4-week storage in three aqueous solutions. Collagenase and esterase solutions did not decrease the bond strength of SB any more than the phosphate buffer solution (P, 0.05). In ...
Aim: This study evaluated the influence of dentin wettability on the immediate and extended microtensile bond strength (mTBS) of a universal adhesive system used in the etch-and-rinse strategy. Methods: Twenty human third molars were selected and divided into four groups according to the adhesive system and dentin wettability. The mTBS values of each group were registered 24 h and one year after adhesive system application and resin composite block build-up (n=30). Data were analyzed by the t-test (p,0.05). Results: When both adhesive systems were compared, there was no statistically significant difference when they were applied following wet bonding (p,0.05). However, the dry bonding reduced μTBS values of the Adper Single Bond 2 adhesive (p,0.05). Regarding storage time, both groups presented similar μTBS values at 24 h and one year (p,0.05). Conclusions: Therefore, the Scotchbond Universal Adhesive can be applied to dry or wet dentin without compromising the etch-and-rinse bonding quality ...
TY - JOUR. T1 - Enamel and Dentin Bond Durability of Self-Adhesive Restorative Materials. AU - Latta, Mark A.. AU - Tsujimoto, Akimasa. AU - Takamizawa, Toshiki. AU - Barkmeier, Wayne W.. PY - 2020/1/1. Y1 - 2020/1/1. N2 - PURPOSE: To use shear bond strength (SBS) and shear fatigue strength (SFS) testing to determine the durability of adhesion of self-adhesive restorative materials compared to composite resin bonded with a universal adhesive. MATERIALS AND METHODS: A universal adhesive, Prime & Bond Active, was used in self-etch mode to bond Z-100 composite resin to enamel and dentin. Three commercially available restorative materials and one experimental material with self-adhesive properties, Activa (A), Fuji II LC(F), and Equia Forte (E) and ASAR-MP4 (S) were also bonded to enamel and dentin. The SBS and SFS were determined for all materials. A staircase method was used to determine the SFS with 10 Hz frequency for 50,000 cycles or until failure occurred. RESULTS: On enamel, S generated ...
This study compared the microtensile bond strength (BS) and the micromorphology of resin-dentin interface (MI) of a conventional adhesive to two-steps etch-and-rinse bonding agents after 1 year of water storage. Twenty-eight human third molars were used (n = 7). Teeth were divided into four groups (GCB: Gluma Comfort Bond; OPB: OptiBond FL; OCB: One Coat Bond SL; PUB: Peak Universal Bond). Specimens were tested in tension after 24 h or 1 year of water storage. Dentin BS strength data were analyzed by split-plot two-way ANOVA followed by Tukey-Kramer tests (α = 0.05). Water-storage for 1 year significantly decreased BS for OCB; however, no significant difference was noted between OCB and GCB and PUB adhesives after 1 year of water-storage. OPB showed the highest BS values at both storage times. All adhesives formed a hybrid layer with resin tags, and both interfacial structures were maintained after 1 year. However, degradation signals within interfacial structures were observed only for the adhesive
Objective. To determine the translucency of human and bovine enamel and dentin using reflection spectrophotometers. Material and Methods. The color of intact human and bovine enamel and dentin specimens was measured over a white and a black plasticine backing using two spectrophotometers with different aperture sizes; translucency parameter (TP) and contrast ratio (CR) were calculated. Plasticine was used as backing because the specimens were not uniform. The TP values of five composite resins measured over the backings of plasticine and reflectance standards were also determined. Results. There were no significant differences between the TP values of the composite resins measured over the backings of plasticine and reflectance standards (p,0.05). Mean TP values of 1 mm thick bovine enamel, bovine dentin, human enamel and human dentin were 14.7, 15.2, 18.7, and 16.4, respectively, based on the 3 mm round aperture. There were significant correlations between the TP values measured by the two ...
TY - JOUR. T1 - Shear bond strength of the Tenure Solution dentin bonding system.. AU - Barkmeier, Wayne W.. AU - Cooley, R. L.. PY - 1989/10. Y1 - 1989/10. N2 - A liquid solution of an oxalate bonding system containing NTG-GMA and PMDM has become commercially available. The bond strength of this oxalate adhesive (Tenure Solution) to dentin was determined by bonding composite resin cylinders to extracted teeth. The bond strengths obtained in this study are compared to the bond strengths obtained in earlier studies with the first and second generation oxalate adhesives whose components were supplied as powders and required mixing. The oxalate solutions developed significantly higher bond strengths than the original powder type systems.. AB - A liquid solution of an oxalate bonding system containing NTG-GMA and PMDM has become commercially available. The bond strength of this oxalate adhesive (Tenure Solution) to dentin was determined by bonding composite resin cylinders to extracted teeth. The ...
Objectives: To develop prototype rotary selective dentin caries excavators and to demonstrate their ability to remove only carious dentin in extracted teeth. Method and materials: Milled polymer prototype and formed wire loop prototype burs were made. They were tested on normal dentin with standardized force application and compared to carbide burs for ability to cut by weighing three extracted teeth at pre- and postcutting for each prototype version. They were tested on carious dentin of three teeth for each prototype version. The resulting excavated surfaces were analyzed with dentin caries dye, the teeth were decalcified and examined histologically, and the used prototypes were examined in light and scanning electron microscopes. Results: For both prototypes, noncarious teeth did not lose weight from prototype instrumentation, but each lost 9 or 10 mg after instrumentation with the control carbide bur. Both prototypes quickly removed carious dentin in each of the carious teeth until a ...
TY - JOUR. T1 - Micro-tensile bond strength of self-etching primer adhesive systems to human coronal carious dentin. AU - Doi, J.. AU - Itota, T.. AU - Torii, Y.. AU - Nakabo, S.. AU - Yoshiyama, M.. PY - 2004/10. Y1 - 2004/10. N2 - The aim of this study was to evaluate the micro-tensile bond strengths of three self-etching primer adhesive systems to normal dentin (ND), caries-affected dentin (CAD) and caries-infected dentin (CID). Human extracted molars with caries were used, and flat dentin surfaces ground by 600-grit SiC paper were prepared. The surfaces were dyed using Caries-Detector solution, treated with Clearfil SE Bond, Mac-Bond II and UniFil Bond, and then covered with resin composites according to manufacturers instructions. After immersion in 37°C water for 24 h, the teeth were serially sectioned into multiple slices. Each slice was distinguished into ND, CAD and CID groups by the degree of staining, and the bond strength was measured in a universal testing machine. Scanning ...
TY - JOUR. T1 - Effect of ammonium hexafluorosilicate on dentin tubule occlusion for the treatment of dentin hypersensitivity. AU - Suge, Toshiyuki. AU - Kawasaki, Akiko. AU - Ishikawa, Kunio. AU - Matsuo, Takashi. AU - Ebisu, Shigeyuki. PY - 2006/8. Y1 - 2006/8. N2 - Purpose: To evaluate the occluding ability of ammonium hexafluorosilicate (SiF). Methods: Dentin disks prepared from human extracted teeth were grouped as follows to prepare different situations of dentin hypersensitivity: (1) those sonicated for 20 minutes; (2) those treated with 0.5 mol/L EDTA for 2 minutes; (3) those treated with 6% citric acid for 2 minutes; (4) those treated with 50% citric acid for 2 minutes. Then, SiF or diamine silver fluoride (AgF) was applied to the dentin disks and the dentin tubule occlusion was observed with scanning electron microscopy (SEM). The percent of open tubules before and after SiF or AgF treatment were measured by NIH image using SEM photographs. Also, the dentin permeability was measured. ...
TY - JOUR. T1 - Microtensile bond strength between glass ionomer cement and silver diamine fluoride-treated carious primary dentin. AU - Puwanawiroj, Aksrapak. AU - Trairatvorakul, Chutima. AU - Dasanayake, Ananda P.. AU - Auychai, Prim. PY - 2018/7/1. Y1 - 2018/7/1. N2 - Purpose: The study objectives were to investigate the effect of silver diamine fluoride (SDF) on the microtensile bond strength between glass ionomer cement and carious primary dentin and evaluate the mode of restoration failure. Methods: Forty carious primary molars were sectioned in half through the middle of the carious lesion and randomly allocated to test and control groups. The test specimens were treated with 38 percent SDF, and the control, deionized water. The samples were stored in artificial saliva for 14 days at 37 degrees Celsius, and the dentin surfaces were conditioned and restored with Fuji IX GP Extra. After 24 hours in artificial saliva, the specimens were prepared for microtensile bond strength testing and ...
At-home treatments include desensitizing toothpastes or dentifrices, potassium salts, mouthwashes and chewing gums. A variety of toothpastes are marketed for dentin hypersensitivity, including compounds such as strontium chloride, strontium acetate, arginine, calcium carbonate, hydroxyapatite and calcium sodium phosphosilicate.[1] Desensitizing chewing gums[19] and mouthwashes are also marketed.[5] Potassium-containing toothpastes are common; however, the mechanism by which they may reduce hypersensitivity is unclear. Animal research has demonstrated that potassium ions placed in deep dentin cavities cause nerve depolarization and prevent re-polarization. It is not known if this effect would occur with the twice-daily, transient and small increase in potassium ions in saliva that brushing with potassium-containing toothpaste creates. In individuals with dentin hypersensitivity associated with exposed root surfaces, brushing twice daily with toothpaste containing 5% potassium nitrate for six to ...
The aim of this study is to investigate the morphological and chemical changes of demineralized dentin collagen-matrix and resin/dentin interface associated with chitosan/riboflavin modification. Dentin disc specimens were prepared from sound molars, acid-etched with 35% phosphoric acid and modified with either 0.1% riboflavin or chitosan/riboflavin (Ch/RF ratios 1:4 or 1:1) and photo-activated by UVA. Morphological and chemical changes associated with surface modification were characterized by SEM and micro-Raman spectroscopy. Dentin surfaces of sound molars were exposed, acid-etched, and modified as described before. Etch-and-rinse dentin adhesive was applied, light-cured, and layered with resin-restorative composite. The resin infiltration and resin/dentin interface were characterized by micro-Raman spectroscopy and SEM. An open-intact collagen network-structure, formation of uniform hybrid-layer and higher resin infiltration were found with 0.1%RF and Ch/RF 1:4 modifications. Raman analysis ...
TY - JOUR. T1 - The Ability of a Potassium Oxalate Gel Strip to Occlude Human Dentine Tubules; A Novel in vitro. T2 - in situ Study. AU - Chapman, Nicholas. AU - Jones, Siân Bodfel. AU - Bahal, Priya. AU - He, Tao. AU - Drake, Phil. AU - Zou, Yuanshu. AU - West, Nicola X. PY - 2020/9/1. Y1 - 2020/9/1. N2 - Abstract Objectives: To determine if an oxalate strip reduced fluid flow in dentine samples and whether this reduction was maintained following a 14 day intra-oral period. Methods: Dentine tubule fluid flow was measured by a modified Pashley cell in 40 acid-etched dentine discs 1mm thick, diameter ,10mm, with an acquired pellicle, pre-equilibrated with Hartmanns solution and conditioned by toothbrushing, pre and post treatment (10min) with an oxalate (3.14%) gel strip or no treatment. One control and one test sample were exposed in-situ for 14 days to the oral environment in 20 healthy adult volunteers, and fluid flow re-measured. The appliance containing the two samples was removed for ...
The aim of this study was to evaluate the microtensile bond strength of two all-in-one self-etch adhesives applied to sound and caries-affected dentin with four different application techniques. Forty extracted third molars with occlusal caries were randomly divided into four groups for G-Aenial Bond and S3 Bond: (1) according to manufacturer-s instructions; (2) with acid etching before applying adhesives; (3) doubling adhesive application time; and (4) doubling adhesive coating. Teeth were sectioned to obtain 1-mm-+/- 0.2-mm-thick dentin sticks and subjected to a tensile force. For G-Aenial Bond, doubling the time and application of two consecutive coats produced significantly higher strength than that obtained by following manufacturers- instructions and acid etching application to sound dentin. Prior acid etching and application of two consecutive coats to caries-affected dentin generated significantly higher bond strength than that using other techniques. For S3 Bond, there was no ...
The aim of this study was to evaluate the regional push-out bond strengths for two fiber-reinforced post types using three different dentin bonding agents Sixty single-rooted extracted human first premolar teeth were sectioned below the cemento-enamel junction, and the roots were endodontically treated Following standardized post space preparations, the roots were divided into two fiber-post groups (Glassix (R) and Carbopost (R)), and further divided into three subgroups of 10 specimens each for the bonding systems self-etching dentin bonding agents (Clearfil (TM) SE Bond and Optibond (R) all-in-one), and total-etching dentin bonding agent (XP Bond (TM)) A dual-cure resin luting cement (Maxcem (TM)) was then placed in the post spaces and posts were then seated into the root canals polymerized through the cervical portion The roots were then cut into 3-mm thick sections Push-out tests were performed at a crosshead speed of 0 5 mm/min. The data were analyzed with multivariate ANOVA (alpha = 0 05) ...
This study evaluated the MMP inhibition of the zinc oxide and copper nanoparticles (ZnO/CuNp), and the effects of their addition into adhesives on antimicrobial activity (AMA), ultimate tensile strength (UTS), in vitro degree of conversion (in vitro-DC), as well as, resin-dentin bond strength (μTBS), nanoleakage (NL) and in situ-DC on caries-affected dentin. Anti-MMP activity was evaluated for several MMPs. ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added into Prime&Bond Active (PBA) and Ambar Universal (AMB). The AMA was evaluated against Streptococcus mutans. UTS were tested after 24 h and 28d. After induced caries, adhesives and composite were applied to flat dentin surfaces, and specimens were sectioned to obtain resin-dentin sticks. μTBS, NL, in vitro-DC and in situ-DC were evaluated after 24 h. ANOVA and Tukeys test were applied (α = 0.05). ZnO/CuNp demonstrated anti-MMP activity (p < 0.05). The addition of ZnO/CuNp increased AMA and UTS (AMB; p < 0.05). UTS for PBA, in ...
This study aimed to characterize fluoride-induced alterations in dentin mineralization within a dentin-pulp organ culture system. Tooth sections derived from male Wistar rat incisors were cultured in Trowel-type culture for 14 days, in the presence of 0 mM, 1 mM, 3 mM and 6 mM sodium fluoride. Tooth sections were processed and analyzed for uptake of fluoride, its subsequent effect on dentin mineralization by tetracycline hydrochloride incorporation and mineral composition, expressed as calcium/phosphorous (Ca/P) ratios. Tetracycline hydrochloride incorporation was demonstrated to decrease with increased fluoride exposure, accompanied by significant increases in both Ca/P ratios and fluoride incorporation. These findings provide further evidence that the established alterations in dentin formation during fluorosis are a consequence of disruption to the mineralization process, and provide a model system with which to investigate further the potential role the extracellular matrix plays in inducing ...
High-temperature materials in service experience mechanical damage due to operating load and metallurgical damage due to operating temperature. When designing or assessing life of high-temperature components, must consider both factors. In this paper, the effect of tensile hold time on high-temperature fatigue crack growth and long-term prior thermal aging heat treatment on creep rupture behavior were investigated by using STS 316L and STS 316, austenitic stainless steels, which are widely used for automotive exhaust and piping of high-temperature component. STS 316L steel was tested for fatigue crack propagation test by applying 0 sec., 5 sec., 10 sec., 50 sec., and 100 sec. tensile hold times in trapezoidal waveform at 600°... and by using the prior aged STS 316, creep rupture tests were conducted under various stress conditions and at various temperatures. In high-temperature fatigue crack growth tests using STS 316L, as tensile hold time increased crack growth rate (da/dN) decreased in ...
Looking for online definition of irregular dentin in the Medical Dictionary? irregular dentin explanation free. What is irregular dentin? Meaning of irregular dentin medical term. What does irregular dentin mean?
0168]Continuous intrafibrillar and interfibrillar remineralization that occurred after 4 weeks are illustrated in FIG. 6. It was difficult to distinguish the remineralized dentin matrix from the underlying intact mineralized dentin using FESEM, except for the absence of peritubular dentin around the orifices of the dentinal tubules (FIG. 6A). FESEM analysis indicated that the remineralized dentin attained enough support to prevent it from collapse or shrinkage during high vacuum SEM examination. Peritubular dentin (open arrow) was absent from the superficial 5 μm of the dentinal tubules. At higher magnification, it was apparent that the majority of the collagen fibrils exhibited the corn-on-the-cob appearance (arrow), representative of interfibrillar mineralization, while other smaller diameter fibrils were devoid of interfibrillar minerals and exhibited only faint banding characteristics (open arrowhead) (FIG. 6B). Low magnification TEM showed that the partially remineralized dentin (open ...
Methodology Single-rooted human premolars were irrigated as follows (n = 12 per group): (1) 1% NaOCl during instrumentation, deionized water after instrumentation, (2) 1% NaOCl during, 17% EDTA after instrumentation, (3) a 1 : 1-mixture of 2% NaOCl and 18% EA during and after instrumentation, and (4) 1% NaOCl during, 2.25% PA after instrumentation. Irrigant volumes and contact times were 10 mL/15 min during and 5 mL/3 min after instrumentation. The evaluated outcomes were eluted calcium by atomic absorption spectroscopy, smear-covered areas by scanning electron microscopy in secondary electron mode and apparent canal wall decalcifications on root transsections in backscatter mode. For the smear layer analysis, sclerotic dentine was taken into consideration. Results were compared using appropriate parametric and nonparametric tests, alpha = 0.05 ...
MTA와 dentin 사이의 계면 결합력을 평가하기 위해 dentin 시편에 MTA를 충전시킨 후 전단강도를 측정하였으며 7개의 단근치와 5개의 다근치의 root 부위를 실험에 이용하였다. 지름 10mm의 플라스틱 mold에 치아 root를 2mm 길이로 절단하여 매몰하고 이를 직경 1.3mm의 diamond bur로 dentin의 canal을 따라 2mm의 depth로 삭제하였다. 원기둥형으로 치아삭제를 하여 20개의 시편을 제작하였고 이 중에서 실험에 적합한 18개의 시편을 선택하였다. 총 24개의 canal에 MTA(ProRoot MTA, Dentsply, Tulsadental, Tulsa, OK, USA)를 증류수, 1.0%, 2.0% chlorhexidine의 3개의 group으로 분류하여 충전하였다. LF-Plus digital testing machine (Lloyd instrument, Ametek)을 이용하여 1mm/min의 속도로 시편에 dislodgement force를 가하였고 MTA부분이 dentin으로부터 분리될 때의 load를 측정했다. SPSS ver. 19.0K를 이용했고, 유의수준(p-value) 0.05를 ...
TY - JOUR. T1 - Identification of the chondrogenic-inducing activity from bovine dentin (bCIA) as a low-molecular-mass amelogenin polypeptide. AU - Nebgen, D. R.. AU - Inoue, H.. AU - Sabsay, B.. AU - Wei, K.. AU - Ho, C. S.. AU - Veis, A.. PY - 1999. Y1 - 1999. N2 - Dentin extracellular matrix has been shown to contain components capable of inducing chondrogenesis and osteogenesis at ectopic sites when implanted in vivo, and chondrogenesis in cultures of embryonic muscle-derived fibroblasts (EMF) in vitro. The polypeptide responsible, called the chondrogenic-inducing agent (CIA), has been isolated from a 4.0-M guanidinium hydrochloride extract of demineralized bovine dentin matrix. Following Sephacryl S-100 chromatography, CIA activity was identified in fractions by assay for uptake of [35S]-SO4 into proteoglycan by the EMF after 24 hrs in culture. The active fraction induced the EMF to produce type II collagen mRNA and decrease production of type I collagen mRNA after 5 days in culture. The ...
Host-derived proteases have been reported to degrade the collagen matrix of incompletely-resinin-filtrated dentin. This study tested the hypothesis that interfacial degradation of resin-dentin bonds may be prevented or delayed by the application of chlorhexidine (CHX), a matrix metalloproteinase inhibitor, to dentin after phosphoric acid-etching. Contralateral pairs of resin-bonded Class I restorations in non-carious third molars were kept under intra-oral function for 14 months. Preservation of resin-dentin bonds was assessed by microtensile bond strength tests and TEM examination. In vivo bond strength remained stable in the CHX-treated specimens, while bond strength decreased significantly in control teeth. Resin-infiltrated dentin in CHX-treated specimens exhibited normal structural integrity of the collagen network. Conversely, progressive disintegration of the fibrillar network was identified in control specimens. Auto-degradation of collagen matrices can occur in resin-infiltrated dentin, ...
Dentinogenesis imperfecta (DI) type II, an inherited disorder affecting dentin, has been linked to mutations in the dentin sialophosphoprotein ( DSPP) gene on chromosome 4q21. The gene product is cleaved into two dentin-specific matrix proteins, dentin sialoprotein (DSP) and dentin phosphoprotein. The aim of this investigation was to study genotypes and phenotypes in two affected families with special reference to clinical, radiographic, and histopathologic manifestations. Seven affected members of Family A and five of Family B were documented clinically and radiographically; 14 and 10 teeth, respectively, were available for histopathologic investigation and prepared for ground sections, which were assessed semiquantitatively for dysplastic manifestations in the dentin according to the scoring system, dysplastic dentin score (DDS). Venous blood samples were collected from six affected and ten unaffected members of Family A, and from eight affected and six unaffected members of Family B. Genomic ...
ABSTRACT. Aim: To evaluate the comparative efficacy of Polymer bur (Smartprep) and Conventional burs (Carbide bur & Diamond Points) in selective Dentin caries removal. Materials and method: 15 freshly extracted carious human premolars and molars were selected for this study. These teeth were split in the center of carious lesion, in order to obtain two corresponding half. Thus 30 samples were prepared in this way. These were randomly divided in to 3 groups of 10 samples each. Group 1: The carious portion of these samples was excavated with the help of polymer bur Smartprep (SS White). Group 3: Prepared with Tungsten carbide round bur (No. 018). Group 3: Prepared with the help of Diamond points (No. 018). The period of time involved in this process is measured. After excavation of all carious lesions, a longitudinal section from mid most part of lesion was obtain with the help of diamond disc at slow speed. After staining with caries detector dye Seek, the sections were subjected to ...
Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. DMP1 is required for bone and dentin mineralization and is expressed in the cells of bone and teeth. It is thought to play a role in regulating expression of osteoblast-specific genes during osteoblast cell differentiation and is localized to the nucleus in osteoblast precursor cells. In mature osteoblasts, the DMP1 protein is phosphorylated and localized to the extracellular matrix, where it plays a role in forming mineralized matrix. While other constitutively expressed proteins in the extracellular matrix, such as osteopontin and osteocalcin, are expressed in osteoblast cells, DMP1 is expressed in osteocytes, making it a candidate biomarker for osteocyte activity.. ...
Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. DMP1 is required for bone and dentin mineralization and is expressed in the cells of bone and teeth. It is thought to play a role in regulating expression of osteoblast-specific genes during osteoblast cell differentiation and is localized to the nucleus in osteoblast precursor cells. In mature osteoblasts, the DMP1 protein is phosphorylated and localized to the extracellular matrix, where it plays a role in forming mineralized matrix. While other constitutively expressed proteins in the extracellular matrix, such as osteopontin and osteocalcin, are expressed in osteoblast cells, DMP1 is expressed in osteocytes, making it a candidate biomarker for osteocyte activity.. ...
Dentin Sensitivity; Dentin Hypersensitivity. On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.
BackgroundLight curing of resin composite is associated with a thermal rise that may have harmful effect on the health of the vital pulp. In addition, desirable polymerization is important to achieve mechanical properties and clinical function. The purpose of this in‑vitro study was to compare the thermal rise under normal dentin during photopolymerization and degree of conversion (DC) of bulk fill and conventional resin composite using continuous high‑ and soft‑start mode.Materials and MethodsIn this in‑vitro study, Cl I cavities with a dimension of 4 mm × 4 mm × 4 mm and remaining dentin thickness of 1 mm were prepared on 56 extracted human molars. The temperature rise during the light curing of conventional resin composite (Tetric N Ceram, Ivoclar Vivadent) by incremental filling technique and bulk‑fill resin composite (Tetric N Ceram Bulk Fill, Ivoclar Vivadent) by bulk‑filling technique were measured with a K‑type thermocouple wire. DC of both resin composites was measured
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The aim of the present study was to evaluate the influence different bleaching agents and concentrations on microtensile bond strength of different dentin adhesives in class V cavities. 135 freshly extracted third molars were included in this study. In all teeth cervical class V dentinal cavities were prepared allowing the simulation of dentin perfusion. The specimens were randomly assigned to nine experimental groups of fifteen each: S0: Syntac/ control; S17: Syntac/ Perfect Bleach 17%; S38: Syntac/ Opalescene Xtra Boost 38%; X0: XP Bond/ control; X17: XP Bond/ Perfect Bleach 17%; X38: XP Bond/ Opalescene Xtra Boost 38%; A0: AdheSE One/ control; A17: AdheSE One/ Perfect Bleach 17%; A38: AdheSE One/ Opalescene Xtra Boost 38%. Bleaching was performed 8 hours at 36°C for 7 days. Between the ranges all samples were stored in physiological saline for 16 hours. The dentin adhesives used as recommended by manufactures instruction were applied on a standardized dentin surface. Microtensile bond ...
Objective: The purpose of this in vitro study was to determine the microtensile bond strengths (µTBS) of 3 dental adhesives when applied to dentin decalcified with ethylenediaminetetraacetic acid (EDTA). The null hypothesis tested was that the removal of calcium from dentin would not influence the bond strengths. Method and materials: Eighteen extracted human molars were cut in 2 equal halves. One half served as the control, having no EDTA treatment, while the other half was assigned to 1 of 3 periods (1 hour, 24 hours, or 100 hours) of decalcificatioin with buffered 0.5 mol/L EDTA. Middle dentin was bonded with 1 of 3 dentin adhesive groups: a self-etching primer, Clearfil SE Bond (SE); an acetone-based total-etch adhesive, Prime&Bond NT (NT); and an ethanol- and water-based total-etch adhesive, Single Bond (SB). The specimens were restored with Z-250 resin composite and sectioned in 0.9 ± 0.2 mm2 sticks that were tested in tensile mode. Results: For each adhesive, the control group (not ...
Fig. (2d) SEM micrograph of the top surface of dentin disc in the BIF-CA Group: The dentin surface was covered with a semi-porous layer (2,000X). ...
Dentin is one of the most durable biological materials in the human body. Researchers from Charité - Universitätsmedizin Berlin were able to show that the reason for this can be traced to its nanostructures and specifically to the interactions between the organic and inorganic components. Measurements performed at BESSYII, the Helmholtz-Zentrum Berlins synchrotron radiation source, showed that it is the mechanical coupling between the collagen protein fibers and mineral nanoparticles which renders dentin capable of withstanding extreme forces. Results from this research have been recently published in the journal Chemistry of Materials*.. In humans, teeth come into contact almost 5,000 times per day under normal use. In spite of this, and although we often use large forces during mastication, it is surprisingly rare for healthy teeth to break. It is widely accepted that the design of teeth makes teeth tough, where an inner core, known as dentin, supports the outer hard enamel cap. The secret ...
TY - JOUR. T1 - Thermo-debonding mechanisms in dentin bonding systems using finite element analysis. AU - Lee, Sheng Yang. AU - Chiang, Hsin Chih. AU - Huang, Haw Ming. AU - Shih, Yung Hsun. AU - Chen, Hsin Chung. AU - Dong, De Rei. AU - Lin, Che Tong. N1 - 被引用次數:24 Export Date: 9 August 2016 CODEN: BIMAD 通訊地址: Lee, S.-Y.; Grad. Inst. Oral Rehabilitation Sci., Taipei Medical College, 250 Wu-Hsing Street, Taipei, Taiwan; 電子郵件: [email protected] 化學物質/CAS: Biocompatible Materials; Dentin-Bonding Agents; Optibond; Resin Cements; Scotchbond Multi-Purpose 商標: Clearfil Protect liner, Kuraray, Japan; Dentin; Hybrid layer; Optibond; Scotchbond MP; Tenure; Z100 製造商: Kuraray, Japan 參考文獻: Van Noort, R., Cardew, G.E., Howard, I.C., A study of the interfacial shear and tensile stresses in a restored molar tooth (1988) J Dent, 16, pp. 286-293; Van Noort, R., Noroozi, S., Howard, I.C., Cardew, G.E., A critique of bond strength measurements (1989) J ...
Tooth sensitivity or known as dentin hypersensitivity is short or transient sharp pain of a rapid onset that arises from exposed dentin layer of one or more teeth subsequent to loss of tooth structure (enamel layer of the crown or cementum layer of the root). It usually occurs in response to stimuli-typically cold, air pressure, drying, sugar, acids, chemicals or forces acting onto the tooth-and cannot be ascribed to any other dental defects or pathology. These stimuli are non-noxious, and are not generally expected to generate a pain response, except as seen in sensitive teeth. In contrast, a noxious stimulus would be the toxins of bacteria within a decay lesion leading to dentinal pain. Areas of exposed dentin at the junction between the crown and the root (cervical area) account for much of the observed tooth sensitivity. Continue reading →. ...
Inaugural-Dissertation (Dr. med. dent.). Marginale Adaptation & Verschleissfestigkeit experimenteller CEREC - Porzellaninlays in vitro (Arbeit unter der Leitung von PD Dr. med. dent. I. Krejci).. Publikationsübersicht. Marending M, Lutz F, Barbakow F. Scanning electron microscope appearances of Lightspeed instruments used clinically: a pilot study. International Endodontic Journal (1998) 31, 57-62.. Marending M, Peters OA, Zehnder M. Factors affecting the outcome of orthograde root canal therapy in a general dentistry hospital practice. Oral Surg Oral Med Oral Pathol Oral Radiol Endod (2005) 99, 119-24.. Marending M, Brunner T, Luder H, Stark WJ, Zehnder M. Effect of sodium hypochlorite on human root dentine - mechanical, chemical and morphological evaluation. International Endodontic Journal (2007) 40, 786-793.. Marending M, Paqué F, Fischer J, Zehnder M. Impact of irrigant sequence on mechanical properties of human root dentin. Journal of Endodontics (2007) 33, 1325-1328.. Marending M, ...
Objectives Contemporary methods of dentin bonding could create hybrid layers (HLs) containing voids and exposed demineralized collagen fibers. and without 5% PA were bonded to the acid etched dentin. Five-μm-thick sections cut from the bonded specimens were stained with Goldners trichrome. The specimens were then exposed to 0.1% collagenase solution for zero one or six days. Following collagenase treatment the specimens were analyzed with SEM/TEM. Results Staining did not SB-220453 reveal a difference in the HLs created with the two adhesives. SEM showed the presence of intact collagen fibrils in all collagenase treatment conditions for specimens bonded with adhesive containing PA. These integral collagen fibrils were not observed in the specimens bonded with adhesive without PA after the same collagenase treatment. TEM confirmed that the specimens containing PA still showed normal collagen fibril organization and dimensions after treatment with collagenase solution. In contrast disorganized ...

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  • the tooth crown is covered instead with vitrodentin , a compound related to dentin, which is harder than dentin but somewhat softer than enamel. (
  • The bulk of a tooth is made up of dentin. (
  • Dentinal sclerosis/transparent dentin-sclerosis of primary dentin is regressive alteration in tooth characterized by calcification of dentinal tubules. (
  • Dentin is derived from the dental papilla of the tooth germ. (
  • After growth of predentin and maturation into dentin, the cell bodies of the odontoblasts remain in the pulp inside the tooth, along its outer wall, and project into tiny tubules in the dentin. (
  • Dentin continues to form throughout life and can be initiated in response to stimuli, such as tooth decay or attrition. (
  • Because of dentinal tubules, dentin has a degree of permeability, which can increase the sensation of pain and the rate of tooth decay. (
  • These data support the previous suggestion that dentin sialoprotein and dentin phosphoprotein have distinct functions related to tooth formation, and that the dentino-enamel junction should be viewed as a unique transition zone between enamel and the underlying dentin. (
  • If you can keep that tooth clean and refreshen the dentin at cementation, you will be able to produce that exact bond. (
  • Wnt-Responsive Odontoblasts Secrete New Dentin after Superficial Tooth Injury. (
  • The objective of our experiments was to identify new therapeutic strategies to stimulate dentin formation in an adult tooth. (
  • GC Tooth Mousse, a complex containing amorphous calcium phosphate (ACP) and casein phosphopeptide (CPP), is recommended in dentin hypersensitivity reduction due to its ability to block opened dentinal tubules. (
  • The present in situ study investigated the influence of a preparation containing CPP/ACP (caseinphosphopeptide-amorphous calcium phosphate) (GC Tooth mousse) on initial bacterial colonization of enamel and dentin. (
  • Results from this research have been recently published in the journal Chemistry of Materials ( 'Water-Mediated Collagen and Mineral Nanoparticle Interactions Guide Functional Deformation of Human Tooth Dentin' ). (
  • Measuring the heat on the opposite side of the dentin slice -- the side that would touch pulp in a living tooth -- the researchers found that in group three, it reached almost 14 degrees Celsius. (
  • A slow but steady (mind) shift from full metal and metal-ceramic restorations that require conventional cementation and substantial tooth loss (Edelhoff en Sorensen, 2002) to less destructive partial all ceramic restorations that require adhesive cementation is seen in the clinical field. (
  • Immediate Dentin Sealing (IDS) is a technique that presumably improves adhesion of ceramic fixed prosthesis to tooth structure which results in a better marginal adaptation to dentin and less postoperative sensitivity compared to conventional adhesive cementation, also referred to as Delayed Dentin Sealing (DDS). (
  • Dentin is the most voluminous tissue of the tooth and its formation (dentinogenesis) is a highly regulated process displaying several similarities with osteogenesis. (
  • A tooth is a complex organ that is composed of calcified tissues (dentin, enamel, and cementum) and soft connective tissues (the dental pulp and the periodontal ligaments) in which blood vessels and nerves are protected. (
  • A method and system for attaching, for example, an artificial dental prosthetic device such as a crown, cap, or bridge to a prepared tooth structure utilizes a fluoride releasing dentin bonding system in combination with a cement layer. (
  • The dentin bonding system is first applied to the prepared tooth structure, the dentin bonding system including a primer layer and a VLC microfill composite resin layer in certain embodiments. (
  • Then the artificial prosthetic device (e.g. crown) is removably adhered to the prepared tooth structure and the dentin bonding system by way of the cement (e.g. zinc oxide based type). (
  • The cement layer sandwiched between the attached device and the dentin bonding system has a shear function less than that of the device and the dentin bonding system but sufficient to permanently bond the device to the tooth and dentin bonding system during masticatory functions and demands, thereby permitting the device to be removed or sheared from the tooth (and dentin bonding system) without destroying either. (
  • You can use it to bond direct and indirect restorations to all tooth surfaces, seal crown preps, assist with core build-ups, and act as a relief agent for patients with dentin hypersensitivity. (
  • The process whose specific outcome is the progression of a dentin-containing tooth over time, from its formation to the mature structure. (
  • Dentin region of a human tooth with canals or dentinal tubules (dental canaliculi), coloured scanning electron micrograph (SEM). (
  • Dentin is mineralized connective tissue below the tooth enamel surface. (
  • The visible part of a tooth (the crown) consists of enamel covered dentin. (
  • It is indicated for treatment of exposed dentin , for use after prophylaxis or scaling and root planing, and before and/or after tooth bleaching. (
  • Recession itself is a condition that predisposes a person to root caries--cavities--due to decay of the exposed dentin on the root of the tooth. (
  • Rather than simply filling a hole, the implants stimulate stem cells to encourage the growth of dentin - the bony material that makes up the majority of the tooth, Newsweek reported. (
  • A decline in pH and dissolution of the inorganic content of the dental tissues are followed by exposure of the organic portion of the tooth, which, in dentin, is largely composed of collagen fibres. (
  • Dentin dysplasia type II (DD-II) is a rare mild form of dentin dysplasia (DD, see this term) characterized by normal tooth roots but abnormal primary dentition. (
  • The similarity of bone to tooth structure in their organic and inorganic composition offers a good prospect of the application of CO 3 Ap for regeneration of dentin and pulp tissue repair. (
  • Therefore, we investigated the role of CRAMP and FPR2 in tooth development, reparative dentin formation, and defense response. (
  • The present study was performed to investigate the mineral density distribution in enamel and dentin for both permanent and primary teeth and to establish the standard density per tooth type using micro-computed tomography (CT). (
  • Cubic regression curves were used to determine the mineral density distribution in the enamel and dentin for each tooth type. (
  • The mean mineral density values for permanent enamel and dentin were significantly higher than those for their primary counterparts for each tooth type. (
  • In the present study, we demonstrated the distribution of mineral density in sound enamel and dentin and attempted to determine the standard mineral density for each tooth type using micro-CT. (
  • As the highly volatile liquids, ether and chloroform, evaporate a certain amount of heat is abstracted from the tooth-structure, and a coating of the zinc chloride, alkaloidal cocaine, or menthol, driven to all remedies will not completely desensitize all dentin, but their use will be a material aid. (
  • [5] Others suggest that due to the presence of patent dentinal tubules in areas of hypersensitive dentin, there may be increased irritation to the pulp, causing a degree of reversible inflammation. (
  • A few animals, such as flounder and cod , have vasodentin, in which tubules are lacking, and the dentin is nourished directly by capillaries. (
  • Dentin consists of microscopic channels, called dentinal tubules, which radiate outward through the dentin from the pulp to the exterior cementum or enamel border. (
  • From the outer surface of the dentin to the area nearest the pulp, these tubules follow an S-shaped path. (
  • The dentinal tubules contain the cytoplasmic extensions of odontoblasts that once formed the dentin and maintain it. (
  • When an injury is superficial, as in the case of a dentin injury model, then disturbances are largely confined to pulp tissue immediately underneath the damaged dentin tubules. (
  • Amplifying Wnt signaling in the pulp stimulates dentin secretion, and in the dentin injury model, we show that a liposomal formulation of human WNT3A protein passes through dentinal tubules and is capable of upregulating Wnt signaling in the pulp. (
  • The dentin tubules (seen in cross section) have been formed by cytoplasmic extensions of the odontoblasts (dentin-producing cells). (
  • Occluding agents act by physically covering or "plugging" the open, exposed dentin tubules, thus preventing the effect of thermal changes or physical stimuli caused by the movement of dentin fluid resulting from pressure changes. (
  • There has been recent concern about the inadvertent exposure of dentin with patent tubules as well as gingiva to bleaching systems containing 10-15% carbamide peroxide or 2-10% hydrogen peroxide for more than a few minutes. (
  • Most dentin tubules in sound dentin appeared open using CLSM, but most dentin tubules in carious dentin appeared closed or narrowed. (
  • In areas where both primary and secondary mineralization have occurred with complete crystalline fusion, these appear as lighter rounded areas on a stained section of dentin and are considered globular dentin. (
  • Overexpression of dentin sialoprotein results in an increased rate of enamel mineralization, however, the enamel morphology is not significantly altered. (
  • CD146 + cells may promote mineralization and generate dentin/pulp-like structures, suggesting a role in self-renewal of stem cells and dental pulp regenerative therapy. (
  • DMP1 is required for bone and dentin mineralization and is expressed in the cells of bone and teeth. (
  • Bevelander, G. and Nakahara, H. (1965), Correlation between tetracycline binding and mineralization in dentin and enamel. (
  • During dentin formation, there are three components that are necessary for proper mineralization, namely, collagen which forms a scaffold, noncollagenous proteins that can specifically bind to the collagen template and function as a mineral nucleator and crystalline calcium phosphate deposited in an ordered manner. (
  • Here we demonstrate by in situ hybridization techniques that the noncollagenous dentin matrix proteins 1, 2 (DMP1, 2) and dentin sialoprotein (DSP) have characteristic temporal and spatial expression patterns within odontogenic tissues during dentin mineralization. (
  • Expression of the SIBLINGs was originally thought to be limited to bone and teeth where they function to facilitate dentin and bone matrix mineralization [1] - [3] . (
  • The gene for dentin sialophosphoprotein produces a single protein that is post-translationally modified to generate two distinct extracellular proteins: dentin sialoprotein and dentin phosphoprotein. (
  • In teeth, dentin sialophosphoprotein is expressed primarily by odontoblast cells, but is also transiently expressed by presecretory ameloblasts. (
  • Because of this expression profile it appears that dentin sialophosphoprotein contributes to the early events of amelogenesis, and in particular to those events that result in the formation of the dentino-enamel junction and the adjacent "aprismatic" enamel. (
  • Immunohistochemical studies detected dentin matrix protein-1 (DMP1) and dentin sialophosphoprotein (DSPP), as well as human mitochondria, in transplanted DPSCs. (
  • DD-II is caused by mutations in the DSPP gene (4q21.3) coding for dentin sialophosphoprotein, a precursor for dentin sialoprotein and dentin phosphoprotein which are involved in dentinogenesis. (
  • We determined recently that dentin sialophosphoprotein (DSPP), a member of the SIBLING (Small integrin-binding ligand N-linked glycoproteins) family of phosphoglycoproteins, is highly upregulated in human oral squamous cell carcinomas (OSCCs) where upregulation is associated with tumor aggressiveness. (
  • Dentin sialophosphoprotein (DSPP) is a member of the SIBLING (Small Integrin-Binding LIgand N-linked Glycoprotein) family of extracellular matrix glycophosphoproteins [1] . (
  • Other members of the family are bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP), osteopontin (OPN), and matrix extracellular phosphoglycoprotein (MEPE) [1] . (
  • Non-dentin layer is covered by enamel or cementum and gingiva. (
  • Dentin (/ˈdɛntɪn/) (American English) or dentine (/ˈdɛnˌtiːn/ or /ˌdɛnˈtiːn/) (British English) (Latin: substantia eburnea) is a calcified tissue of the body and, along with enamel, cementum, and pulp, is one of the four major components of teeth. (
  • Dentin without the enamel or cementum that normally covers it. (
  • A degree of dentin sensitivity is normal, but pain is not usually experienced in everyday activities like drinking a cooled drink. (
  • Therefore, although the terms dentin sensitivity and sensitive dentin are used interchangeably to refer to dental hypersensitivity, [5] the latter term is the most accurate. (
  • After the teeth isolation with cotton rolls, changes in the dentin sensitivity to tactile (dental explorer), thermal stimuli (drops of melted ice) and air stimuli (blast from dental syringe) will be evaluated. (
  • It is presumed that IDS both reduces postoperative sensitivity and increases the bond strength to dentin as compared to DDS. (
  • The results of a PubMed search reveal that many more publications focus on the therapy of dentin (hyper)sensitivity than on diagnostic aspects. (
  • It is made up, by weight, of 72% inorganic materials (mainly hydroxylapatite and some non-crystalline amorphous calcium phosphate), 20% organic materials (90% of which is collagen type 1 and the remaining 10% ground substance, which includes dentin-specific proteins), and 8% water (which is adsorbed on the surface of the minerals or between the crystals). (
  • Measurements performed at BESSYII, the Helmholtz-Zentrum Berlin's synchrotron radiation source, showed that it is the mechanical coupling between the collagen protein fibers and mineral nanoparticles which renders dentin capable of withstanding extreme forces. (
  • Dentin is a bone-like substance, which is composed of mineral nanoparticles, collagen and water. (
  • Even at the lowest setting, the ablation may weaken bond strength by denaturing collagen in dentin, she said. (
  • Control beams were not exposed to PA but were incubated in simulated body fluid for 3 days to assay their total endogenous telopeptidase activity, by their ability to solubilize C-terminal crosslinked telopeptides ICTP and CTX from insoluble dentin collagen. (
  • Objective To evaluate the effect of dentin matrix metalloproteinase(MMP) on the degradation of root dentin collagen. (
  • CONCLSUION:The K + and acetic acid have synergetic effect on the solubility of root dentin collagen. (
  • Objective The purpose of this study was to evaluate the effect of degradation of human root dentin collagen treated with collagenase after demineralization. (
  • In control defects collagen matrix alone failed to form complete dentin bridges. (
  • Finally, if you seal the dentin before making the impression, the impression includes the contours of the adhesive. (
  • Developing an effective adhesive bond to dentin is more difficult due to the chemical and biological complexity o f dentin. (
  • Many techniques and means have been developed with the purpose to adhesive chemical bond o f composite resin to organic and anorganic components o f dentin. (
  • S. E. M. Saber and F. S. El-Askary, "The outcome of immediate or delayed application of a single-step self-etch adhesive to coronal dentin following the application of different endodontic irrigants," Eur J Dent , vol. 3, pp. 83-89, 2009. (
  • Effect of green tea extract on bonding durability of an etch-and-rinse adhesive system to caries-affected dentin," Journal of Applied Oral Science , vol. 24, no. 3, pp. 211-217, 2016. (
  • Immediate Dentin Sealing as an adjunct to adhesive cementation of all ceramic restorations is proposed and executed in several studies, but there is very limited evidence to support its effectiveness. (
  • Although several studies have evaluated the immediate bond strength of simplified adhesive systems to permanent and primary dentin , the results obtained are still controversial. (
  • Do the origins of primary teeth affect the bond strength of a self-etching adhesive system to dentin? (
  • We evaluated the influence of protease inhibitors on the bond strength of a self-etch adhesive system to caries-affected dentin. (
  • Bond strength test is crucial for evaluation of dentin-adhesive effectiveness and for informing the design of adhesive systems. (
  • For the dentin-adhesive interface, this type of test can be applied to not only access the tensile behavior but also behavior under complex combined normal and shear loading conditions. (
  • The new adhesive showed a degree of double bond conversion and mechanical properties comparable with control, with good penetration into the dentin surface and a uniform adhesive/dentin interface. (
  • Conclusions: NaF and KF treatment after acid-etching step of adhesive bonding procedures do not adversely influence the initial or long-term bond strength to dentin, and can improve the maintenance of bond strength durability. (
  • Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. (
  • Anti-Dentin Matrix Protein-I antibody is a rabbit polyclonal antibody raised against the KLH-conjugated peptide (90-111) derived from the N-terminus of rat DMP1. (
  • DMP1, DMP2 and DSP mRNA are expressed in the odontoblasts at specific and overlapping time points and are thus presumably used for different functions during dentin formation. (
  • Dentin matrix protein 1 (DMP1) has been known for a number of years to bind to CD44 and ArgGlyAsp sequence-dependent integrins. (
  • Dentin matrix protein 1 (DMP1) is an acidic phosphoglycoprotein and member of the integrin-binding SIBLING protein family ( 1 ). (
  • Dentin , also spelled dentine , in anatomy , the yellowish tissue that makes up the bulk of all teeth . (
  • It is widely accepted that the design of teeth makes teeth tough, where an inner core, known as dentin, supports the outer hard enamel cap. (
  • Standardized blocks of dentin obtained from sound permanent teeth extracted for orthodontic reasons were demineralized with Ethylenediaminetetraacetic acid (EDTA) and pretreated either with (A) GSE (0.2% w/v), (B) amine fluoride (AmF) (20% w/v), (C) a mouthrinse which contains both, (D) placebo, (E) sodium fluoride (0.15 −1 ), (F) PBS, (G) Chlorhexidine digluconate (CHX), or (H) zinc chloride (ZnCl 2 ). (
  • S. Hergüner Siso, K. Er, F. Hürmüzlü, A. Kustarci i K.E. Akpinar, "Fracture Resistance of Root-Filled Maxillary Premolar Teeth Restored with Current Dentin Bonding Adhesives", Acta stomatologica Croatica , vol.42, br. (
  • Objective: The aim was to study and compare the fracture resistance of root-filled premolar teeth restored with various dentin bonding adhesives (DBAs). (
  • 13 In endodontics, however, there is no standard solution for testing bioceramic materials, or any studies that determine what would be the best solution for in vitro simulation of natural dentin moisture in vivo for endodontically treated teeth. (
  • Materials and methods: Sixty dentin blocks, 2-3 mm thick and 4 mm in diameter, were prepared from developing teeth of young pigs. (
  • After the teeth were embedded and sectioned (400 m), the caries in the remaining dentin was assessed using a caries detector. (
  • The first group including 3 dogs 11 months old in which we fractured all third incisors, canines, fourth premolars and third upper molars (84 teeth together), showed by the F-test, significantly different results in pulp vitality resulting in dentin production compared with second group. (
  • Fractured teeth in the early stages of maturation, consisting of 40-50% hard tissue, have much higher ability to continue dentin deposition despite chronic pulp inflammation. (
  • By comparison, teeth in the second year of life consist of more then 60% hard tissue, have a lower ability to survive, and significantly decreased dentin production. (
  • Human teeth were embedded in acrylic resin and then polished until the dentin surface became exposed. (
  • Two layers of carious dentin from extracted human teeth were observed with an electron microscope. (
  • The enamel and dentin mineral densities in the extracted teeth were measured using micro-CT. (
  • In hOP-1 treated teeth substantial amounts of hard tissue formation (osteodentin and tubular dentin) had consistently led to a complete bridging of the defects. (
  • The problem of the desensitization of the dentin of teeth comes to the forefront from time to time. (
  • In cases where cavities have been prepared in vital teeth under conduction anesthesia, or for a jacket crown, the exposed dentin is generally hypersensitive after the anesthesia disappears. (
  • Materials and methods: Mid-coronal dentin surfaces of ninety-nine teeth were etched with 32%wt phosphoric acid and randomly assigned to pretreatment with NaF, KF or CaF2 solutions (6, 24 and 179 mM F content) for 30 s before the application of bonding agent (Scothbond Multi-Purpose, 3 M ESPE). (
  • secondly, dentin is sensitive and can become hypersensitive to changes in temperatur due to the sensory function of odontoblasts, especially when enamel recedes and dentin channels become exposed. (
  • The formation of dentin, known as dentinogenesis, begins prior to the formation of enamel and is initiated by the odontoblasts of the pulp. (
  • The cell bodies of the odontoblasts are aligned along the inner aspect of dentin against a layer of predentin where they also form the peripheral boundary of the dental pulp. (
  • R26R mTmG/+ reporter strain was then used to demonstrate that a population of long-lived Wnt-responsive odontoblasts, which secreted dentin throughout the life of the animal, were responsible for depositing new dentin in response to a superficial injury. (
  • Expression of the transgene transcript and beta-galactosidase activity were restricted to dentin and odontoblasts with spatial and temporal patterns comparable to those of the endogenous mouse Dsp transcript, although beta-galactosidase activity could not be detected visually during embryonal stages. (
  • Dentin-forming cells, odontoblasts, which originate from the ectomesenchyme, form a single layer of cells between the dentin and pulp. (
  • In the study, both DPSCs and BM-MSCs that were transplanted into immunocompromised mice successfully generated either dentin/pulp-like structures or bone tissue-like structures. (
  • TY - JOUR T1 - Differential expression patterns of the dentin matrix proteins during mineralized tissue formation. (
  • Dentin consists of inorganic hard tissue and organic dentin matrix components (DMCs). (
  • To understand the mechanism of radiotherapy-induced dental lesions characterized by shear fracture of enamel near the dentin-enamel junction (DEJ) that suggests decreased interface stability. (
  • What is enamel-dentin-pulp fracture and how is it treated? (
  • Fracture involves enamel and dentin and the pulp is exposed. (
  • By volume, 45% of dentin consists of the mineral hydroxyapatite, 33% is organic material, and 22% is water. (
  • While both enamel and dentine are composed of the same mineral called carbonated hydroxyapatite (cHAP), dentin represents a complex nanocomposite material. (
  • We synthetized dentin-like scaffolds using gelatin biomineralized with magnesium-doped hydroxyapatite and blended it with alginate. (
  • 2 These new composites generally contain bioactive glasses capable of releasing ionic species and forming a precipitate, hydroxyapatite, allowing for better sealing by inducing dentin remineralization, in addition to inhibiting microbial biofilm formation. (
  • This study evaluated the effect of mechanical cycling on the bond strength of zirconia posts to root dentin. (
  • It was concluded that the mechanical cycling damaged the bond strength of zirconia posts to root dentin. (
  • 4 However, no studies have evaluated the bond strength of ceramic posts to root dentin after mechanical cycling, simulating a masticatory load. (
  • Considering the present knowledge gap on the resistance to fatigue of ceramic posts adhesively cemented, the aim of this study was to evaluate the effect of mechanical cycling on the push-out bond strength between root dentin and zirconium post. (
  • To evaluate the influence of rewetting solutions on bond strength to root dentin of conventional gutta-percha (GP) or niobium phosphate glass-based gutta-percha (GNb) associated with a bioceramic sealer. (
  • Methods The root dentin samples were demineralized by incubating with pH5.0 lactate acid at 370C. (
  • Methods Root dentin powder was demineralized with acetic acid (pH4.0) at 4 ℃ for 14 d, then dialysed and centrifuged. (
  • The mechanical and optical properties of healthy and transparent root dentin are compared using atomic force microscopy (AFM), micro-Raman and emission spectroscopies and fluorescence microscopy. (
  • De-Deus, G., Paciornik, S., Pinho Mauricio, M. and Prioli, R. (2006) Real-time atomic force microscopy of root dentin during demineralization when subjected to chelating agents. (
  • Discussion: how can we improve diagnosis of dentin hypersensitivity in the dental office? (
  • Current diagnosis of dentin hypersensitivity in the dental office: an overview. (
  • OBJECTIVES: The aim of this overview is to consider the problems that may be associated with making a diagnosis of dentin hypersensitivity (DHS) and to provide a basis for clinicians to effectively diagnose and manage this troublesome clinical condition. (
  • Kowalczyk A, Botuliński B, Jaworska M, Kierklo A, Pawińska M, Dabrowska E. Evaluation of the product based on Recaldent technology in the treatment of dentin hypersensitivity. (
  • Objective: Dentin desensitizing agents are used in the treatment of dentin hypersensitivity, which is defined as a painful response in the exposed dentin to stimuli that are thermal, evaporative, tactile, osmotic, or chemical. (
  • Dentin sialoprotein and dentin phosphoprotein overexpression during amelogenesis. (
  • Our findings indicate that protein S100-A7 released from dentin by MMP20 might play a key role in dentin pulp regeneration. (
  • 6 There exists a rich cocktail of bioactive molecules in dentin and pulp capable of giving the signaling events involved in dentin and pulp regeneration. (
  • Using a transgenic animal approach we have extended dentin sialoprotein or dentin phosphoprotein expression throughout the developmental stages of amelogenesis. (
  • In contrast, the overexpression of dentin phosphoprotein creates "pitted" and "chalky" enamel of non-uniform thickness that is more prone to wear. (
  • Disruptions to the prismatic enamel structure are also a characteristic of the dentin phosphoprotein overexpressing animals. (
  • Objectives: This study was undertaken to examine the influence of partial demineralization of xenogenous dentin on bone formation in an osteoconductive environment. (
  • Conclusion: Partial demineralization of xenogenous dentin blocks may provide a method for optimizing the integration of dentin onlays in an osteoconductive environment, thus stabilizing the implant and slowing down replacement resorption. (
  • Purpose: To evaluate the effect of artificial demineralization upon color change of dentin in contact with dental amalgam. (
  • Clinical significance: Deposition of black corrosion products into dentin was strongly related to dentin demineralization. (
  • This study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. (
  • This is the peer reviewed version of the following article: Park, J.-G., Ye, Q., Topp, E. M. and Spencer, P. (2009), Enzyme-catalyzed hydrolysis of dentin adhesives containing a new urethane-based trimethacrylate monomer. (
  • A new trimethacrylate monomer with urethane-linked groups, 1,1,1-tri-[4-(methacryloxyethylamino-carbonyloxy)-phenyl]ethane (MPE), was synthesized, characterized, and used as a co-monomer in dentin adhesives. (
  • In his latest paper ( Journal of Prosthetic Dentistry , September 2007, Vol. 98:3, pp. 166-174), Dr. Magne and his colleagues prepared 30 extracted human molars and immediately bonded their dentin: 15 with OptiBond FL ( Kerr Dental ) and 15 with SE Bond ( Kuraray ). (
  • For comparison purposes, they placed Tempfil Inlays on 10 molars without bonding the dentin first, and soaked these in saline solution for two weeks before using either OptiBond FL or SE Bond to attach the Z100. (
  • Extracted human molars were bonded and prepared for microtensile dentin bond strength (microTBS) testing using Prompt L-Pop, EBS Multi, and Prime&Bond NT combined with Pertac II (composite) or Hytac Aplitip (compomer). (
  • Dentin bonding agents provide the most appropriate conditions for bonding o f composite resins to acid etched dentin surface and they have been developed through a few generations with continual improvements in bond strength and marginal sealing. (
  • We "acid-etched" experimental groups by exposing the demineralized dentin beams to 1, 10, or 37 mass% PA for 15 sec or 15 min. (
  • Dentin hypersensitivity is defined as a short, sharp pain arising from exposed dentin in response to stimuli, which cannot be ascribed to any other form of dental defect or pathology. (
  • To contradict this view, not all exposed dentin surfaces cause DH. (
  • In Group 1, the exposed dentin surfaces were conditioned with GC cavity conditioner for a duration of 10 s, washed and air dried but not desiccated. (
  • It is also worth considering that high failure rates of ceramic laminate veneers have been related to large exposed dentin surfaces [13,18]. (
  • The exposed dentin surfaces were sequentially polished with 240, 320, 450, and 600 grit sand paper. (
  • Caries study: Dentin surfaces, some with pre-existing caries, were visualized using CLSM. (
  • A dispersive high-Cu amalgam or conventional low-Cu amalgam was condensed onto dentin surfaces of all groups. (
  • Biodentine™ is a dentin substitute indicated for use in the crown for temporary enamel restorations, permanent dentin restorations, deep or large carious lesions, deep cervical or radicular lesions, pulp capping or pulpotomy. (
  • The aim of this study was to assess the effect of CPP-ACP combined with fluoride varnish in cervical dentin hypersensitivity treatment. (
  • Cervical dentin hypersensitivity (CDH) and noncarious cervical lesions (NCCLs) are common findings in modern clinical practice. (
  • Effect of phosphoric acid on the degradation of human dentin matrix. (
  • Unlike enamel, dentin may be demineralized and stained for histological study. (
  • Histological examinations revealed an increased area of dentin/pulp-like structures in transplanted CD146 + cells, compared with CD146 − and CD146 +/− cells. (
  • In this study, the CO 3 Ap cement is used to cover an exposed dental pulp in rats and histological evaluation was carried out to evaluate the formation of reparative dentin, which is one of the signs of positive pulp capping treatment outcome. (
  • The Evaluation of formation of reparative dentin was done in 3 weeks after application by histological observation. (
  • Reparative dentin was histologically visible for all samples in CO 3 Ap group, as well as in the Ca(OH) 2 groups. (
  • The formation of reparative dentin was observable in 3 weeks of evaluation. (
  • Next, we investigated the localization of CRAMP, FPR2, and CD68-positive macrophages by immunohistochemical analysis during pulp inflammation and reparative dentin formation after cavity preparation. (
  • At subsequent stages of reparative dentin formation, CRAMP was observed in odontoblast-like cells that contacted reparative dentin. (
  • Localization of CRAMP and its receptor FPR2-positive cells were observed during physiological and reparative dentin formation. (
  • CRAMP/LL-37 has a possibility that induce reparative dentin formation. (
  • Whether CPP-ACP can act synergistically with fluorine in the dentin hypersensitivity treatment is unknown. (
  • An in vitro model was used to examine the effects of radiotherapy, elapsed time following radiation, and occlusal function on the nano -mechanical properties of enamel and dentin near the DEJ. (
  • This preliminary evidence suggests that radiation, in conjunction with elapsed time and occlusal load, has a significant effect on the nano -mechanical properties of enamel and dentin near the DEJ. (
  • The mineral density distributions found in this study contribute to our understanding of the mechanical properties of enamel and dentin. (
  • Dr. Barros and her colleagues at the University of Michigan (where she was formerly based), used infrared thermography and microtensile bond strength (MTBS) analysis to study the potential effects of Er,Cr:YSGG lasers on dentin adhesion and pulp. (
  • The objective of this study was to evaluate the effect of fluoride pretreatment on the immediate and long-term microtensile bond strength (mu TBS) of resin-dentin interfaces. (
  • The clinical success of ceramic restorations relies heavily on the quality of their adhesion to dentin, which remains a clinical challenge to date. (
  • A noticeable finding was that two of the restorations (1 IBSE and 1 CLSE) had failed in the same patient, and in both cases, the restorations had fractured leaving a large piece of the composite still bonded to the dentin. (
  • The shade and translucency of the IPS Style Ceram Dentin materials have been adjusted to simulate natural dentine. (
  • DenMat offers traditional phosphoric acid formulas as well as phosphoric acid and aluminum oxalate combined so you can etch enamel and condition dentin at the same time. (
  • In contrast, the organic fibers found in dentin appear to exert exactly the right pressure on the mineral nanoparticles that is required to increase the material's repetitive, cyclic load-bearing capacity," argue the scientists. (
  • These results support the notion that the dentin proteins expressed by presecretory ameloblasts contribute to the unique properties of the dentino-enamel junction. (
  • The supernatants were analyzed by Western Blot for several dentin matrix proteins known to be MMP-3 substrate. (
  • The bioactive polymer used in UNO forms a rapid, protective, dentin integrated layer to prevent hypersensitivity", said Dr. Kishen. (
  • 12 Nevertheless, there are still no studies relating the combined use of bioceramic sealers and bioactive gutta-percha and their effects on the bond strength to dentin. (
  • This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146 + ) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146 − ) counterparts. (
  • Therefore, pellicle formation was performed in situ on bovine enamel and dentin specimens fixed to individual upper jaw splints worn by 8 subjects. (
  • It was the aim of the present study to investigate the induction of dentin formation by recombinant human osteogenic protein-1 (hOP-1). (
  • Less dentin formation was seen after calcium hydroxide application. (
  • Five different dual cure resin cements were used for cementing rods to bovine dentin. (
  • In the treatment of hypersensitive dentin Phenol Compound is highly efficacious. (
  • Comparision of different conditioners and its effect on human dentin and on Light Activated Glass Ionomer Cements. (
  • Secondary dentin, a less well-organized form of tubular dentin, is produced throughout life as a patching material where cavities have begun, where the overlying enamel has been worn away, and within the pulp chamber as part of the aging process. (
  • Though more efficient nutritionally, this type of dentin is softer and less resistant to disease than tubular dentin. (
  • To address this objective, we evaluated dentin production in 2 acute trauma models: one involving a pulp exposure and the other involving a superficial dentin injury. (
  • One reason IDS is stronger is that dentin bonds best to composite when the dentin is freshly cut, Dr. Magne said. (
  • Dr. Barros' group is doing further research to see if various etches remove the denatured layers of dentin, exposing a surface that can better bond to the composite resin. (
  • Data from literature demonstrates that adhesion force of composite resin systems, as applied to primary dentin , ranges from 5.53 to 70.1 MPa. (
  • Dentin rates approximately 3 on the Mohs scale of mineral hardness. (
  • In wild-type animals, the inclusion of dentin sialoprotein in the forming aprismatic enamel may account for its increased hardness properties, when compared with bulk enamel. (
  • Increases the hardness of the dentinal surface up to 10 times more than dentin coated with conventional bonding agents! (
  • Cures to a Barcol hardness of 72 - the typical hardness of dentin. (
  • That's why we created Absolute Dentin to cure to a Barcol hardness of 72, the typical hardness of dentin (hence the name). (
  • One experienced clinician performed all excavation procedures, monitoring the caries removal by checking the hardness of the dentin with a dental explorer. (
  • Dentin is a bone-like matrix that is porous and yellow-hued material. (
  • To evaluate debonding mechanism of zirconia and lithium disilicate cemented to dentin mimicking what could occur in a clinical setting. (
  • Since Matrix metalloproteinases (MMPs) have been suggested to contribute to dentin caries progression, the hypothesis that MMP inhibition would affect the progression of dentin caries is clinically relevant. (
  • To evaluate the capacity of a GSE mouthrinse to prevent the degradation of demineralized dentin matrix by MMP-3 (stromelysin-1). (
  • Western blot analysis of the supernatants revealed that MMP-3 released from the dentin matrix small proteoglycans (decorin and biglycan) and dentin sialoprotein (DSP) in the AmF, sodium fluoride, PBS and placebo pretreated groups, but not in the GSE and mouthrinse pretreated groups. (
  • This study shows that GSE either alone or combined with AmF as in the evaluated mouthrinse limits dentin matrix degradation. (
  • Indeed, cariogenic bacteria create acidic environment, exposing the dentin matrix. (
  • hence enabling further dentin matrix degradation. (
  • The use of sodium trimetaphosphate as a biomimetic analog of matrix phosphoproteins for remineralization of artificial caries-like dentin. (
  • This study examined the use of sodium trimetaphosphate (STMP) as a biomimetic analog of matrix phosphoproteins for remineralization of artificial carious-affected dentin. (