Dextrans: A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes.Dextran Sulfate: Long-chain polymer of glucose containing 17-20% sulfur. It has been used as an anticoagulant and also has been shown to inhibit the binding of HIV-1 to CD4-POSITIVE T-LYMPHOCYTES. It is commonly used as both an experimental and clinical laboratory reagent and has been investigated for use as an antiviral agent, in the treatment of hypolipidemia, and for the prevention of free radical damage, among other applications.DEAE-Dextran: Used as a support for ion-exchange chromatography.Chromatography, DEAE-Cellulose: A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)DEAE-Cellulose: Cellulose derivative used in chromatography, as ion-exchange material, and for various industrial applications.Chromatography, Ion Exchange: Separation technique in which the stationary phase consists of ion exchange resins. The resins contain loosely held small ions that easily exchange places with other small ions of like charge present in solutions washed over the resins.Molecular Weight: The sum of the weight of all the atoms in a molecule.Chromatography: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Ion Exchange Resins: High molecular weight, insoluble polymers which contain functional groups that are capable of undergoing exchange reactions (ION EXCHANGE) with either cations or anions.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Iron-Dextran Complex: A complex of ferric oxyhydroxide with dextrans of 5000 to 7000 daltons in a viscous solution containing 50 mg/ml of iron. It is supplied as a parenteral preparation and is used as a hematinic. (Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1292)RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Colitis: Inflammation of the COLON section of the large intestine (INTESTINE, LARGE), usually with symptoms such as DIARRHEA (often with blood and mucus), ABDOMINAL PAIN, and FEVER.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Composition: The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Fruiting Bodies, Fungal: The fruiting 'heads' or 'caps' of FUNGI, which as a food item are familiarly known as MUSHROOMS, that contain the FUNGAL SPORES.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)DextranaseFluorescein-5-isothiocyanate: Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Immunoelectrophoresis: A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Kinetics: The rate dynamics in chemical or physical systems.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Ammonium Sulfate: Sulfuric acid diammonium salt. It is used in CHEMICAL FRACTIONATION of proteins.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.PolysaccharidesTemperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Logical Observation Identifiers Names and Codes: A vocabulary database of universal identifiers for laboratory and clinical test results. Its purpose is to facilitate the exchange and pooling of results for clinical care, outcomes management, and research. It is produced by the Regenstrief Institute. (LOINC and RELMA [Internet]. Indianapolis: The Regenstrief Institute; c1995-2001 [cited 2002 Apr 2]. Available from http://www.regenstrief.org/loinc)Actinomycetales: An order of gram-positive, primarily aerobic BACTERIA that tend to form branching filaments.Colon: The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Isoelectric Focusing: Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Immunodiffusion: Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.Electrophoresis: An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Centrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Seawater: The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Korea: Former kingdom, located on Korea Peninsula between Sea of Japan and Yellow Sea on east coast of Asia. In 1948, the kingdom ceased and two independent countries were formed, divided by the 38th parallel.Sodium Chloride: A ubiquitous sodium salt that is commonly used to season food.Quinones: Hydrocarbon rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Geologic Sediments: A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)Cell Line: Established cell cultures that have the potential to propagate indefinitely.Leuconostoc: A genus of gram-positive, facultatively anaerobic bacteria whose growth is dependent on the presence of a fermentable carbohydrate. It is nonpathogenic to plants and animals, including humans.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Vitamin K 2: A group of substances similar to VITAMIN K 1 which contains a ring of 2-methyl-1,4-naphthoquinione and an isoprenoid side chain of varying number of isoprene units. In vitamin K 2, each isoprene unit contains a double bond. They are produced by bacteria including the normal intestinal flora.Dictionaries as Topic: Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.Famous PersonsIsoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Intestinal Mucosa: Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.Enzyme Stability: The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Capillary Permeability: The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.Pigments, Biological: Any normal or abnormal coloring matter in PLANTS; ANIMALS or micro-organisms.Plasma Substitutes: Any liquid used to replace blood plasma, usually a saline solution, often with serum albumins, dextrans or other preparations. These substances do not enhance the oxygen- carrying capacity of blood, but merely replace the volume. They are also used to treat dehydration.Laryngeal Edema: Abnormal accumulation of fluid in tissues of any part of the LARYNX, commonly associated with laryngeal injuries and allergic reactions.Classification: The systematic arrangement of entities in any field into categories classes based on common characteristics such as properties, morphology, subject matter, etc.Permeability: Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.Laryngismus: A disorder in which the adductor muscles of the VOCAL CORDS exhibit increased activity leading to laryngeal spasm. Laryngismus causes closure of the VOCAL FOLDS and airflow obstruction during inspiration.Patient Identification Systems: Organized procedures for establishing patient identity, including use of bracelets, etc.Hemodilution: Reduction of blood viscosity usually by the addition of cell free solutions. Used clinically (1) in states of impaired microcirculation, (2) for replacement of intraoperative blood loss without homologous blood transfusion, and (3) in cardiopulmonary bypass and hypothermia.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Aerobiosis: Life or metabolic reactions occurring in an environment containing oxygen.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Mice, Inbred C57BLErythrocyte Aggregation: The formation of clumps of RED BLOOD CELLS under low or non-flow conditions, resulting from the attraction forces between the red blood cells. The cells adhere to each other in rouleaux aggregates. Slight mechanical force, such as occurs in the circulation, is enough to disperse these aggregates. Stronger or weaker than normal aggregation may result from a variety of effects in the ERYTHROCYTE MEMBRANE or in BLOOD PLASMA. The degree of aggregation is affected by ERYTHROCYTE DEFORMABILITY, erythrocyte membrane sialylation, masking of negative surface charge by plasma proteins, etc. BLOOD VISCOSITY and the ERYTHROCYTE SEDIMENTATION RATE are affected by the amount of erythrocyte aggregation and are parameters used to measure the aggregation.Fluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Ficoll: A sucrose polymer of high molecular weight.
Another method is the use of cationic polymers such as DEAE-dextran or polyethylenimine (PEI). The negatively charged DNA binds ... This transfection technology performs the same tasks as other biochemical procedures utilizing polymers, DEAE-dextran, calcium ...
DEAE-Dextran (DEAE-D) is a positively charged dextran derivative that can be used for vaccine production, gene therapy, protein ... DEAE-Sepharose, DEAE-650 and DEAE-Sephadex are commonly used in chromatography. DEAE-C is a weak anion exchanger. This exchange ... "DEAE and CM Bio-Gel ® A Ion Exchange Gels Instruction Manual" (PDF). Bio-Rad. Retrieved 11 May 2016. "DEAE-Dextran" (PDF). GE ... Gulick, Tod (2003). "Transfection Using DEAE-Dextran". doi:10.1002/0471143030.cb2004s19. Ninfa, Alexander (2010). Fundamental ...
DEAE is an anion exchange matrix that is produced from a positive side group of diethylaminoethyl bound to cellulose or ... Agarose gel based medium contain large pores as well but their substitution ability is lower in comparison to dextrans. The ... Mini columns were filled with DEAE-Sephadex A-50 and further eluted with tris- buffer sodium chloride at various concentrations ... Examples of functional groups of Weak ion exchange resins include diethylaminoethyl (DEAE), which is an anion exchanger, and ...
Methods of artificial transfection include (a) chemical methods, including calcium phosphate precipitation, DEAE-dextran ...
... s can be made from a number of different materials including DEAE-dextran, glass, polystyrene plastic, acrylamide, ... Several types of microcarriers are available commercially including alginate-based (GEM, Global Cell Solutions), dextran-based ...
... deae-dextran MeSH D09.698.365.272.300 --- dextran sulfate MeSH D09.698.365.272.400 --- iron-dextran complex MeSH D09.698. ... deae-cellulose MeSH D09.698.365.180.515 --- lignin MeSH D09.698.365.180.663 --- methylcellulose MeSH D09.698.365.180.663.329 ... carboxymethylcellulose MeSH D09.698.365.272 --- dextrans MeSH D09.698.365.272.250 --- ...
DEAE-Sepharose CL-6B treatment, ammonium sulfate fractionation, dextran sulfate-agarose chromatography, gel filtration on ACA- ... 44, and DEAE-Sephacel chromatography. The structure (primary structure) of protein C inhibitor was deduced from its cDNA ...
DEAE is an anion exchange matrix that is produced from a positive side group of diethylaminoethyl bound to cellulose or ... Agarose gel based medium contain large pores as well but their substitution ability is lower in comparison to dextrans. The ... Examples of functional groups of Weak ion exchange resins include diethylaminoethyl (DEAE, -C2H4N(CH2H5)2), which is an anion ... Mini columns were filled with DEAE-Sephadex A-50 and further eluted with tris- buffer sodium chloride at various concentrations ...
Hi, , , does anyone have any experience with transient transfection of Cos-7 , cells? I have attempted a DEAE-dextran/ ... Transient transfection of Cos-7 (electroporation v DEAE dextran). Rick Nicholson R.Nicholson at cfi.unsw.edu.au Tue Feb 18 22: ... Next message: Transient transfection of Cos-7 (electroporation v DEAE dextran) * Messages sorted by: [ date ] [ thread ] [ ... Next message: Transient transfection of Cos-7 (electroporation v DEAE dextran) * Messages sorted by: [ date ] [ thread ] [ ...
... dextran tightly associates with the backbone of the nucleic acid allowing it to adhere to the cell membrane and enter the ... Diethylaminoethyl (DEAE)-dextran is a polycationic derivative of the carbohydrate polymer dextran, and it is one of the first ... Mix nucleic acid with DEAE-dextran solution in transfection medium or phosphate-buffered saline solution. Nucleic acid-DEAE- ... Mix nucleic acid with DEAE-dextran solution in transfection medium or phosphate-buffered saline solution. Nucleic acid-DEAE- ...
Use of bacterial and firefly luciferases as reporter genes in DEAE-dextran-mediated transfection of mammalian cells.. Pazzagli ...
A DEAE-dextran-MMA copolymer (DDMC)-paclitaxel complex was generated using paclitaxel as the guest and DDMC as the host. The ... Onishi Y, Eshita Y, Mizuno M (2010) DEAE-Dextran and DEAE-Dextran-MMA graft copolymer for nanomedicine.Polymers Research ... 2 g of 2-diethylaminoethyl (DEAE)-dextran hydrochloride (nitrogen content 3%) derived from dextran (Mw 500,000) was dissolved ... A DEAE-dextran-MMA copolymer (DDMC)-paclitaxel complex was generated using paclitaxel as the guest and DDMC as the host. The ...
... (Protocol summary only for purposes of this preview site). ... Transfection mediated by DEAE-dextran differs from calcium phosphate coprecipitation in three important respects. First, it is ... Panel: DEAE-Dextran Transfection1201 *Panel: Electroporation1203 *Chapter 16: Introducing Genes into Mammalian Cells: Viral ... Protocol 4: Transfection Mediated by DEAE-Dextran: High-Efficiency Method *Protocol 5: DNA Transfection by Electroporation * ...
DEAE-dextran, Calcium Phosphate, Activated Dendrimers, Magnetic Beads, Liposomes, Microinjection, Electroporation, Particle ... DEAE-dextran. 5.9.1. DEAE-dextran Market Estimates and Forecasts, 2012 - 2022 (USD Million). 5.10. Magnetic Beads. 5.10.1. ... DEAE-dextran market, 2012 - 2022 (USD Million). 20. Magnetic beads market, 2012 - 2022 (USD Million). 21. Activated dendrimers ... DEAE-dextran. Magnetic beads. Activated Dendrimers. Laserfection. Global Transfection reagents & equipment Application Outlook ...
DEAE-Dextran (Diethylaminoethyl-Dextran) is a polycatonic derivative of Dextran. DEAE-Dextran is used for a number of ... www.dextran.com/products/dextran-derivatives/deae-dextran-500. Additional information:. Anne Marie Oerkild. International Sales ... "Our DEAE-Dextran is graced with the same high quality as our other dextran derivatives, and we believe that we by introducing ... About Deae Dextran. Dextran is used in a number of areas, among other ophthalmic, oral application/tablets, vaccines, ...
... CAS No. 9015-73-0. DEAE-substituents have been introduced into a range of dextran fractions. The chemical ... DEAE-dextrans are supplied as a white hygroscopic powder which are readily soluble in water and salt solutions. The powder is ...
DEAE-Dextran 500 is a of with CAS Registry No: 9015-73-0 and is used for research and analytical purpose.CLEARSYNTH delivers ... Page Keywords:DEAE-Dextran 500, DEAE-Dextran 500,cas#=9015-73-0, COA of DEAE-Dextran 500, MSDS of DEAE-Dextran 500. We deliver ... DEAE-Dextran 500. DEAE-Dextran 500 has CAS Registry No: 9015-73-0. Availability: For Exact delivery date, please contact [email protected] ... Have questions about DEAE-Dextran 500 or Require Pricing?. Let us help you.. Our automated system will send you a quote for ...
Influence of DEAE-dextran for the enhancement of IBR virus infectivity in different cell culture systems, 25 microg DEAE- ... 2- In the roller system, DEAE-dextran enhanced the growth of virus by about 0.9, 0.7 log[10] more than growth of untreated ... 1- Infectivity titre of IBR virus was markedly increased under the effect of both serial passages and DEAE-dextran in MDBK and ... 3- The highest titre obtained when using DEAE-dextran MDBK roller system was log[10] 9.2 TCID[50]/ml ...
Adjuvant effect of DEAE-dextran and tetanus toxoid on whole cell heat inactivated phenol preserved typhoid vaccine. Indian ... Adjuvant effect of DEAE-dextran and tetanus toxoid on whole cell heat inactivated phenol preserved typhoid vaccine. ... or DEAE-Dextran (DD). Immunization of mice with whole cell typhoid vaccine showed enhanced potency either when administered in ...
DEAE Dextran Cationic. 0.80M NaNO3. Polyvinylamine Cationic. 0.80M NaNO3. ...
FITC-DEAE-Dextran, average mol wt 40,000 MilliporeSigma. Synonyms: FITC-DEAE-Dextran 40; Fluorescein isothiocyanate- ...
Plaques with DEAE dextran or protamine sulfate 7.7 (a) Vero (CL) SM 2 3 4+ 3 2-3 mm 9.3 ...
COS cells were transfected using DEAE-dextran (12). For luciferase assays, U138 cells were transfected with 2 μg of luciferase ...
... cationized dextran. Tissue Eng. 13:313-22.CrossRefPubMedGoogle Scholar ...
DEAE-Dextran / pharmacology. Electron Spin Resonance Spectroscopy. Manganese / pharmacokinetics*. Saccharomyces cerevisiae / ...
173:137 (1991)) by the DEAE-dextran method. After 24 h, the cells were trypsinized and transferred to 35 mm dishes (Falcon- ...
Binding of the polycation DEAE-dextran to the cell surface of HA-1 CHO cells caused a marked increase in45Ca2+exchange influx. ... The profound effect of DEAE-dextran on cellular Ca2+uptake and the rapid reversal of the effect by dextran sulfate might make ... DEAE-dextran inhibited the capping of concanavalin-A acceptor proteins. Inhibition of capping occurred over the same DEAE- ... Binding of polycation DEAE-dextran to chinese hamster ovary cells induces reversible Ca2+influx and inhibits capping of ...
Polysacchardies serve as adjuvants (DEAE Dextran, etc.) and immunomodulators. They also can function as unique T cell- ... This was different from dextran phosphate (DP-40) [Suzuki et al., Proceedings of the Society for Experimental Biology and ... Cancer, 16:682-685 (1960); Suemasu et al., Gann, 61(2):125-130 (1970)], sulfated laminaran and dextran [Jolles et al., British ...
DEAE-dextran treatment (Gopal, Mol. Cell Biol., 5:1188-1190(1985)), and particle bombardment (Yang et al., Proc. Natl. Acad. ...
Polyethylenimine and DEAE-dextran are examples of such polymers.. Polycationic small synthetic organic amines that are suitable ...
Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. J Infect Dis 1972;125:665-8. ...
Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. J Infect Dis 1972;125:665-8. ...
Introduction of the construct into the host cell can be effected by calcium phosphate transfection, DEAE-Dextran ... 7 (E. J. Murray, ed., Humana Press) (1991)); DEAE-dextran; electroporation; cationic liposome-mediated transfection; and ...
  • DEAE-substituents have been introduced into a range of dextran fractions. (tdbcons.com)
  • Dextran fractions do not appear to be included in the lists of permitted additives (ingredients) for pharmaceutical formulations such as ointments and creams for topical use and tablets and capsules for oral use. (lookchem.com)
  • 1- Infectivity titre of IBR virus was markedly increased under the effect of both serial passages and DEAE-dextran in MDBK and VERO cells which showed an increase of log 1.2 and log 1.0, respectively more than their respective non treated passages. (bvsalud.org)
  • 2- In the roller system, DEAE-dextran enhanced the growth of virus by about 0.9, 0.7 log more than growth of untreated virus in both MDBK and VERO cell lines, respectively. (bvsalud.org)
  • The API was and is still available in powder and/or bulk solutions for the manufacturing of veterinary iron dextran injection. (pharmacosmos.com)
  • The company is the only dextran provider approved by the US FDA and EDQM in Europe, and Pharmacosmos operates on the basis of an innovative and unique manufacturing process without the use of organic solvents. (pharmacosmos.com)