Dansyl Compounds: Compounds that contain a 1-dimethylaminonaphthalene-5-sulfonyl group.Spectrometry, Fluorescence: Measurement of the intensity and quality of fluorescence.Uranium Compounds: Inorganic compounds that contain uranium as an integral part of the molecule.Fluorescence Polarization: Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.Cadaverine: A foul-smelling diamine formed by bacterial decarboxylation of lysine.Fluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.Energy Transfer: The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Phosphatidylcholines: Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.Nucleic Acids: High molecular weight polymers containing a mixture of purine and pyrimidine nucleotides chained together by ribose or deoxyribose linkages.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Peptide Nucleic Acids: DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Microfluidic Analytical Techniques: Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.Microfluidics: The study of fluid channels and chambers of tiny dimensions of tens to hundreds of micrometers and volumes of nanoliters or picoliters. This is of interest in biological MICROCIRCULATION and used in MICROCHEMISTRY and INVESTIGATIVE TECHNIQUES.Dimethylpolysiloxanes: Silicone polymers which consist of silicon atoms substituted with methyl groups and linked by oxygen atoms. They comprise a series of biocompatible materials used as liquids, gels or solids; as film for artificial membranes, gels for implants, and liquids for drug vehicles; and as antifoaming agents.Lab-On-A-Chip Devices: Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.Equipment Design: Methods of creating machines and devices.Microchip Analytical Procedures: The preparation and analysis of samples on miniaturized devices.PrintingNeurosciences: The scientific disciplines concerned with the embryology, anatomy, physiology, biochemistry, pharmacology, etc., of the nervous system.Amyloid: A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Amyloid beta-Peptides: Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.Psychoanalysis: The separation or resolution of the psyche into its constituent elements. The term has two separate meanings: 1. a procedure devised by Sigmund Freud, for investigating mental processes by means of free association, dream interpretation and interpretation of resistance and transference manifestations; and 2. a theory of psychology developed by Freud from his clinical experience with hysterical patients. (From Campbell, Psychiatric Dictionary, 1996).Neurosurgery: A surgical specialty concerned with the treatment of diseases and disorders of the brain, spinal cord, and peripheral and sympathetic nervous system.Nucleic Acid Probes: Nucleic acid which complements a specific mRNA or DNA molecule, or fragment thereof; used for hybridization studies in order to identify microorganisms and for genetic studies.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Coccidiosis: Protozoan infection found in animals and man. It is caused by several different genera of COCCIDIA.Neospora: A genus of protozoan parasites of the subclass COCCIDIA. Its species are parasitic in dogs, cattle, goats, and sheep, among others. N. caninum, a species that mainly infects dogs, is intracellular in neural and other cells of the body, multiplies by endodyogeny, has no parasitophorous vacuole, and has numerous rhoptries. It is known to cause lesions in many tissues, especially the brain and spinal cord as well as abortion in the expectant mother.Abortion, Veterinary: Premature expulsion of the FETUS in animals.Cattle Diseases: Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.Antibodies, Protozoan: Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Folic Acid: A member of the vitamin B family that stimulates the hematopoietic system. It is present in the liver and kidney and is found in mushrooms, spinach, yeast, green leaves, and grasses (POACEAE). Folic acid is used in the treatment and prevention of folate deficiencies and megaloblastic anemia.Mass Spectrometry: An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.Lung Neoplasms: Tumors or cancer of the LUNG.Carcinoma, Non-Small-Cell Lung: A heterogeneous aggregate of at least three distinct histological types of lung cancer, including SQUAMOUS CELL CARCINOMA; ADENOCARCINOMA; and LARGE CELL CARCINOMA. They are dealt with collectively because of their shared treatment strategy.Chromatography, Liquid: Chromatographic techniques in which the mobile phase is a liquid.Spectrometry, Mass, Electrospray Ionization: A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.Silanes: Compounds similar to hydrocarbons in which a tetravalent silicon atom replaces the carbon atom. They are very reactive, ignite in air, and form useful derivatives.Fluorescence Resonance Energy Transfer: A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.Tandem Mass Spectrometry: A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.Polycyclic Hydrocarbons, Aromatic: A major group of unsaturated cyclic hydrocarbons containing two or more rings. The vast number of compounds of this important group, derived chiefly from petroleum and coal tar, are rather highly reactive and chemically versatile. The name is due to the strong and not unpleasant odor characteristic of most substances of this nature. (From Hawley's Condensed Chemical Dictionary, 12th ed, p96)Complex Mixtures: Mixtures of many components in inexact proportions, usually natural, such as PLANT EXTRACTS; VENOMS; and MANURE. These are distinguished from DRUG COMBINATIONS which have only a few components in definite proportions.Chromatography, Gas: Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.

Turn scanning by site-directed mutagenesis: application to the protein folding problem using the intestinal fatty acid binding protein. (1/423)

We have systematically mutated residues located in turns between beta-strands of the intestinal fatty acid binding protein (IFABP), and a glycine in a half turn, to valine and have examined the stability, refolding rate constants and ligand dissociation constants for each mutant protein. IFABP is an almost all beta-sheet protein exhibiting a topology comprised of two five-stranded sheets surrounding a large cavity into which the fatty acid ligand binds. A glycine residue is located in seven of the eight turns between the antiparallel beta-strands and another in a half turn of a strand connecting the front and back sheets. Mutations in any of the three turns connecting the last four C-terminal strands slow the folding and decrease stability with the mutation between the last two strands slowing folding dramatically. These data suggest that interactions between the last four C-terminal strands are highly cooperative, perhaps triggered by an initial hydrophobic collapse. We suggest that this trigger is collapse of the highly hydrophobic cluster of amino acids in the D and E strands, a region previously shown to also affect the last stage of the folding process (Kim et al., 1997). Changing the glycine in the strand between the front and back sheets also results in a unstable, slow folding protein perhaps disrupting the D-E strand interactions. For most of the other turn mutations there was no apparent correlation between stability and refolding rate constants. In some turns, the interaction between strands, rather than the turn type, appears to be critical for folding while in others, turn formation itself appears to be a rate limiting step. Although there is no simple correlation between turn formation and folding kinetics, we propose that turn scanning by mutagenesis will be a useful tool for issues related to protein folding.  (+info)

N-dansyl-S-nitrosohomocysteine a fluorescent probe for intracellular thiols and S-nitrosothiols. (2/423)

The fluorescence emission spectrum of N-dansyl-S-nitrosohomocysteine was enhanced approximately 8-fold upon removal of the NO group either by photolysis or by transnitrosation with free thiols like glutathione. The fluorescence enhancement was reversible in that it could be quenched in the presence of excess S-nitrosoglutathione. Attempts were then made to utilize N-dansyl-S-nitrosohomocysteine as an intracellular probe of thiols/S-nitrosothiols. Fluorescence microscopy of fibroblasts in culture indicated that intracellular N-dansyl-S-nitrosohomocysteine levels reached a maximum within 5 min. N-Dansyl-S-nitrosohomocysteine fluorescence was directly proportional to intracellular GSH levels, directly determined with HPLC. N-Dansyl-S-nitrosohomocysteine preloaded cells were also sensitive to S-nitrosoglutathione uptake as the intracellular fluorescence decreased as a function of time upon exposure to extracellular S-nitrosoglutathione.  (+info)

Ascaridia galli fatty acid-binding protein, a member of the nematode polyprotein allergens family. (3/423)

A fatty acid-binding protein from the nematode Ascaridia galli was characterized. The gene was isolated and recombinantly expressed in Escherichia coli. According to the deduced amino acid sequence A. galli fatty acid-binding protein (AgFABP) belongs to the family of nematode polyprotein allergens, as shown by Western blotting and PCR analysis with genomic DNA and cDNA. Both native and recombinant proteins bind fatty acids and retinoids with high affinity. The fluorescent fatty acid analogue 11-[(5-dimethylaminonaphthalene-1-sulfonyl)amino] undecanoic acid (DAUDA) shows substantial changes in its emission spectrum when bound to AgFABP; this binding is reversed by fatty acids such as oleate. Moreover, changes of the intrinsic fluorescence of retinol and retinoic acid confirm retinoid binding activity of AgFABP. Fluorescence titration experiments with DAUDA indicate stoichiometric binding to a single binding site per monomer unit with affinities (Kd) of 1.6 and 1.8 x 10(-7) m for native and the recombinant protein, respectively. The apparent binding affinities of the nonfluorescent ligands were calculated in displacement experiments with DAUDA and values in the same range were obtained for myristic, palmitic, oleic, linoleic, arachidonic and retinoic acid. Additionally, the binding affinity of AgFABP for oleate and palmitate was determined by direct and indirect radiochemical analysis and the values obtained were similar to those from the fluorescent experiments. Both proteins show a preference for the binding of long-chain saturated and unsaturated fatty acids, but not for short chain (C3-C12) and branched fatty acids, cholesterol and tryptophan.  (+info)

Divalent cation-, nucleotide-, and polymerization-dependent changes in the conformation of subdomain 2 of actin. (4/423)

Conformational changes in subdomain 2 of actin were investigated using fluorescence probes dansyl cadaverine (DC) or dansyl ethylenediamine (DED) covalently attached to Gln41. Examination of changes in the fluorescence emission spectra as a function of time during Ca2+/Mg2+ and ATP/ADP exchange at the high-affinity site for divalent cation-nucleotide complex in G-actin confirmed a profound influence of the type of nucleotide but failed to detect a significant cation-dependent difference in the environment of Gln41. No significant difference between Ca- and Mg-actin was also seen in the magnitude of the fluorescence changes resulting from the polymerization of these two actin forms. Evidence is presented that earlier reported cation-dependent differences in the conformation of the loop 38-52 may be related to time-dependent changes in the conformation of subdomain 2 in DED- or DC-labeled G-actin, accelerated by substitution of Mg2+ for Ca2+ in CaATP-G-actin and, in particular, by conversion of MgATP- into MgADP-G-actin. These spontaneous changes are associated with a denaturation-driven release of the bound nucleotide that is promoted by two effects of DED or DC labeling: lowered affinity of actin for nucleotide and acceleration of ATP hydrolysis on MgATP-G-actin that converts it into a less stable MgADP form. Evidence is presented that the changes in the environment of Gln41 accompanying actin polymerization result in part from the release of Pi after the hydrolysis of ATP on the polymer. A similarity of this change to that accompanying replacement of the bound ATP with ADP in G-actin is discussed.  (+info)

Interactive binding to the two principal ligand binding sites of human serum albumin: effect of the neutral-to-base transition. (5/423)

The relationship between the two principal ligand binding sites, sites I and II, on human serum albumin (HSA) was quantitatively and qualitatively examined by equilibrium dialysis and fluorescence spectroscopy. Among the three subsite markers to site I, only the binding of dansyl-L-asparagine (DNSA), which is a subsite Ib marker (K. Yamasaki et al., Biochim. Biophys. Acta 1295 (1996) 147), was inhibited by the simultaneous binding of a site II ligand, such as ibuprofen and diazepam. This indicates that, in contrast to subsite Ib, subsites Ia and Ic do not strongly interact with site II. The thermodynamic characteristics for the coupling reaction between DNSA and ibuprofen and between DNSA and diazepam, which gave positive coupling free energies and negative values for both coupling enthalpy and entropy, indicated that the reaction process was entropically driven. Increase of pH from 6.5 to 8.2 caused an increase in coupling constant and entropy for the mutual antagonism between DNSA and the site II ligands on binding to HSA. The site II ligand-induced red-shift of lambda(max) and solvent accessibility of DNSA in subsite Ib were decreased when the albumin molecule was isomerized from the neutral (N) to the base (B) conformation in the physiological pH region. Based on these findings, we conclude that a 'competitive' like strong allosteric regulation exists for the binding of these two ligands to the N conformer, whereas for the B conformer this interaction can be classified as nearly 'independent'. Since the distance between Trp-214, which resides within the site I subdomain, and Tyr-411, which is involved in site II, is increased by 6 A during the N-B transition (N.G. Hagag et al., Fed. Proc. 41 (1982) 1189), we propose a mechanism for the pH-dependent antagonistic binding between subsite Ib and site II, which involves the transmission of ligand-induced allosteric effects from one site to another site, modified by changes in the spatial relationship of sites I and II caused by the N-B transition.  (+info)

A fluorescence energy transfer study of lecithin-cholesterol vesicles in the presence of phospholipase C. (6/423)

We demonstrate Forster resonance energy transfer from dehydroergosterol to dansylated lecithin in lecithin-cholesterol vesicles and characterize the vesicles in the presence of the pro-nucleating enzyme, phospholipase C (PLC). Exposure to phospholipase C causes a temporary decrease in the dehydroergosterol to dansyl fluorescence ratio followed by an increase to and above the initial value. The temporary decrease in the fluorescence ratio results from an increase in the dansylated lecithin intensity that coincides with a dansyl blue shift. The extent of the blue shift correlates with the level of diacylglycerol generated in situ by PLC, suggesting an increased association between dansylated lecithin and cholesterol as membrane fluidity increases and membrane polarity decreases. The subsequent increase in the fluorescence ratio results from both an increase in the dehydroergsterol intensity and a concomitant decrease in the dansylated lecithin intensity of equal magnitude. This signifies a reduction in energy transfer from dehydroergosterol to dansylated lecithin and indicates an increased separation between the two fluorophores. The increase in the fluorescence ratio persists beyond the time scales for vesicle aggregation and fusion, as measured by turbidity, and precedes the onset of macroscopic cholesterol crystals observed with an optical microscope. Thus, the increased separation between dehydroergosterol and dansylated lecithin is consistent with a mechanism of cholesterol nucleation from the vesicles. Moreover, the onset and rate of increase in the fluorescence ratio correlate with the cholesterol:lecithin mole ratio of the vesicles. Fluorescence energy transfer from dehydroergosterol to dansylated lecithin therefore shows potential as a methodology for measuring cholesterol nucleation in model bile.  (+info)

Enantioselective binding sites on bovine serum albumin to dansyl amino acids. (7/423)

The enantioselective binding sites on bovine serum albumin were examined by HPLC using 19 racemic 5-N, N-dimethylamino-1-naphthalenesulfonyl derivatives of alpha-amino acids (dansyl amino acids) as chiral probes. On a bovine serum albumin bonded chiral stationary phase, seven L-forms eluted faster than their D-forms, while ten D-forms eluted before their L-forms. It was speculated that either two classes or two different binding sites exist on bovine serum albumin which can be distinguished by N-dansyl-L-proline and N-dansyl-D-norvaline. This was confirmed by fluorometric experiments where non-fluorescent 1-naphthalenesulfonyl derivatives were synthesized and competitive adsorption experiments were performed.  (+info)

Characterization of the ternary complex between Rab7, REP-1 and Rab geranylgeranyl transferase. (8/423)

Geranylgeranylation is a post-translational modification of Rab GTPases that enables them to associate reversibly with intracellular membranes. Geranylgeranylation of Rab proteins is critical for their activity in controlling intracellular membrane transport. According to the currently accepted model for their action, newly synthesized Rab proteins are recruited by Rab escort protein (REP) and are presented to the Rab geranylgeranyl transferase (RabGGTase) which covalentely modifies the Rab protein with two geranylgeranyl moieties. After prenylation, the Rab protein remains in complex with REP and is delivered to the target membrane by the latter. In this work, we show that RabGGTase can form a stable complex with Rab7-REP in the absence of its lipid substrate geranylgeranyl pyrophosphate. In order to characterize this interaction, we developed three fluorescence assays reporting on the interaction of RabGGTase with the Rab7-REP complex. For this interaction we determined a Kd value of about 120 nM. Association of RabGGTase with the Rab7-REP complex occurs with a rate constant of approximately 108 M-1 x s-1. We demonstrate that the state of the nucleotide bound to Rab7 does not influence the affinity of RabGGTase for the Rab7-REP-1 complex. Finally, we address the issue of substrate specificity of RabGGTase. Titration experiments demonstrate that, in contrast with farnesyl transferase, RabGGTase does not recognize a defined C-terminal sequence motif. Experiments using Rab7 mutants in which the last 16 amino acids were either mutated or truncated revealed that the distal part of the C-terminus makes only a limited contribution to the binding affinity between RabGGTase and the Rab7-REP-1 complex. This demonstrates the functional dissimilarity between RabGGTase and geranylgeranyl transferase I and farnesyl transferase, which interact specifically with the C-terminus of their substrates. Based on these experiments, we propose that RabGGTase recognizes the overall structure arising from the association of Rab and REP and then 'scans' the flexible C-terminus to position the proximal cysteines into the active site.  (+info)

Dansylated analogues of the potent and selective μ opioid peptide agonist [Dmt1]DALDA (H-Dmt-D-Arg-Phe-Lys-NH2; Dmt = 2',6'-dimethyltyrosine) were prepared either by substitution of Nβ-dansyl-α,β-diaminopropionic acid or Nε-dansyllysine for Lys4, or by attachment of a dansyl group to the C-terminal carboxamide function via a linker. All three analogues displayed high μ agonist potency in vitro and the C-terminally dansylated one retained significant μ receptor selectivity. The three analogues showed interesting differences in their fluorescence emission maxima and quantum yields, indicating that the dansyl group in two of them was engaged in intramolecular hydrophobic interactions. These dansylated [Dmt1]DALDA analogues represent valuable tools for binding studies, cellular uptake and intracellular distribution studies, and tissue distribution studies ...
P-Nitro Benzene Sulfonyl Chloride manufacturers - Shree Shraddha Chemicals Pvt .Ltd. exporters & suppliers of P-Nitro Benzene Sulfonyl Chloride, P-Nitro Benzene Sulfonyl Chloride, P-Nitro Benzene Sulfonyl Chloride manufacturer, P-Nitro Benzene Sulfonyl Chloride exporter from Ankleshwar.
Name: 4-Aminobenzene-1-sulfonyl chloride CA Name: Benzenesulfonyl chloride,4-amino- Molecular Structure: 4-Aminobenzene-1-sulfonyl chloride,Benzenesulfonyl chloride,4-amino-,CAS 24939-24-0,191.64,C6H6ClNO2S 4-Aminobenzene-1-sulfonyl chloride,Benzenesulfonyl chloride,4-amino-,CAS 24939-24-0,191.64,C6H6ClNO2S Molecular Formula:C6H6ClNO2S Molecular Weight: 191.64 CAS Registry Number: 24939-24-0
Name: 3-Amino-4-methylbenzene-1-sulfonyl chloride CA Name: Molecular Structure: 3-Amino-4-methylbenzene-1-sulfonyl chloride,205.66,C7H8ClNO2S 3-Amino-4-methylbenzene-1-sulfonyl chloride,205.66,C7H8ClNO2S Molecular Formula:C7H8ClNO2S Molecular Weight: 205.66 CAS Registry Number:
Isoquinoline-5-sulfonyl chloride hydrochloride chemical properties, What are the chemical properties of Isoquinoline-5-sulfonyl chloride hydrochloride 105627-79-0, What are the physical properties of Isoquinoline-5-sulfonyl chloride hydrochloride ect.
Shop a large selection of Sulfonyl halides products and learn more about 3,5-Dichlorobiphenyl-4-sulfonyl chloride, 97%, ACROS Organics™ 1g 3,5-Dichlorobiphenyl-4-sulfonyl
What this means: A protein can consist of 2 or more polypeptide chains that are linked together by disulfide bonds (see graphic below). For sequencing to properly work, you must break all protein subunits into individual strands.. N-terminal analysis of your protein provides information on the different types of subunits.. Since each polypeptide chain has an N-terminus (also known as the "end group" of a protein) it is possible to use a fluorescent compound such as 5-dimethylamino-1-naphthalenesulfonyl chloride (aka "dansyl chloride") to cleave to the primary amine (N-terminal of a protein is the amine group). Quick Recap: N-terminal vs C-terminal of amino acids ...
market. The report also provides the global market segmentation based on applications, end-users, technology, and geography.. The report includes rigorous data, in-depth analysis in two ways, namely, quantitative and qualitative, industry professionals inputs and data given by the industry members and industry analysts involved in the complete value cycle. The report features thorough study of important market and their current trends, coupled with respective market segments. Information about the numerous factors and their influence on the global market and its segments is also mentioned in the report. Check Free Sample Research Report @ http://www.mrsresearchgroup.com/report/97201#request-sample. The report emphasizes on the regional market, the keyplayers in the market, and the various market segments with an in-depth analysis on different divisions and their applications. The report offers inclusive data on the respective segments of the Global Benzene Sulfonyl Chloride (CAS 98-09-9 ...
7-Azabicyclo[4.2.0]octa-1,5-diene-7-sulfonyl chloride, 2-hydroxy-8-oxo- | C7H6ClNO4S | CID 71391929 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
Order 7-Bromo-2,1,3-benzothiadiazole-4-sulfonyl chloride, CAS 41512-06-5, EC-000.1630, MW C6H2BrClN2O2S2 from direct supplier at the best price
Both complement and Fc regions of IgA 1 have been shown to enhance the uptake of antigens by antigen presenting cells (Fang, et. aI., 1998, Foged, et. aI., 2005, Perrin-Cocon, et. aI., 2004). However, the utilization of complement and IgA1 antibodies in enhancing the uptake of antigens by dendritic cells has not been examined to date. The aims of this research included attaching complement to antidansyl IgA 1 antibodies followed by induction, activation, and examination of dendritic cells incubated with the antigen-antibody immune complexes to determine potential interactive relationships. Complement was linked to the Fc region of anti-dansyl IgA 1 antibodies using a procedure to activate complement while the IgA 1 was bound to a dansylated Sepharose 4B affinity column. The eluted complement-coated IgA 1 antibodies were mixed with dansylated albumin to form the complement-coated IgA1-immune complexes. Then, the immune complexes were incubated with PBMCderived dendritic cells to induce maturation ...
A facile one-step Cu(I)-catalyzed "click" reaction, between a dansyl-azide and a propargyl-substituted rhodamine B hydrazide, is employed to fabricate a novel FRET ratiometric "off-on" fluorescent probe. The sensitive emission of the donor, a dansyl group, overlaps perfectly with the absorption of the acceptor, xanthene in the open-ring rhodamine. The proposed probe shows high selectivity towards Cu2+. The ratio of emission intensities at 568 and 540 nm (I-568/I-540) exhibits a drastic 28-fold enhancement upon addition of Cu2+. The probe shows an excellent linear relationship between emission ratios and the concentrations of Cu2+ from 10 to 50 mu M, with a detection limit (S/N = 3) of 0.12 mu M. The preliminary cellular studies demonstrated that the probe is cell membrane permeable and could be applied for ratiometric fluorescence imaging of intracellular Cu2+ with almost no cytotoxicity. The ingenuity of the probe design is to construct a FRET donor-acceptor interconnector and a selective ...
Lookchem Provide Cas No.84255-31-2 Basic information: Properties,Safety Data,Sds and Other Datebase. We also Provide Trading Suppliers & Manufacture for 84255-31-2 N-[[5-(dimethylamino)-1-naphthyl]sulphonyl]-DL-leucine, compound with piperidine (1:1).
The chloride-chloride exchange reaction in arenesulfonyl chlorides was investigated experimentally and theoretically by density functional theory (DFT) calculations. The second order rate constants and activation parameters of this identity reaction were determined for 22 variously substituted arenesulfonyl chlorides using radio-labeled Et4N36Cl. The chloride exchange rates of 11 sulfonyl chlorides bearing para-and meta-substituents (σ constants from −0.66 to +0.43) in the aromatic ring followed the Hammett equation with a ρ-value of +2.02. The mono- and di-ortho-alkyl substituted sulfonyl chlorides exhibit an enhanced reactivity although both inductive and steric effects lower the reaction rate. The DFT calculations of their structures together with X-ray data showed that an increased reactivity is mainly due to a peculiar, rigid, strongly compressed and sterically congested structure. The DFT studies of the title reaction revealed that it proceeds via a single transition state
Novel compounds, e.g. 3-cyano-5,7-dimethyl-N-(2,6-dichlorophenyl)pyrazolo[1,5-a]pyrimidine-2-sul fonamide and their use as herbicides and/or for the suppression of nitrification of ammonium nitrogen i
Causes serious eye damage.. P260-P264b-P271-P280-P301+P330+P331-P303+P361+P353-P304+P340-P305+P351+P338-P310-P363-P501c. H314-H335. DOTInformation : Transport Hazard Class: 8; Packing Group: III; Proper Shipping Name: CORROSIVE LIQUID, ACIDIC, ORGANIC, N.O.S.. TSCA : No. Recommended Storage : Ambient temperatures. ...
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The National Institute of Standards and Technology (NIST) uses its best efforts to deliver a high quality copy of the Database and to verify that the data contained therein have been selected on the basis of sound scientific judgment. However, NIST makes no warranties to that effect, and NIST shall not be liable for any damage that may result from errors or omissions in the Database ...
library 4: ~145,508 Sulfonyl Chloride + Boc-diamine + acrylaic acid library filter4-1677.sulfonyl_chlorides.smi the most common 1677 Enamine sulfonyl_chlorides fro Enamine 83-Boc_diamines.smi the most common 83 Boc from Enamine two step synthesis python SN1-diamines-CO2H.py filter4-1677.sulfonyl_chlorides.smi 83-Boc_diamines.smi python step2-reaction-acrylic-acid.py in.smi pyton step3-remove_doubles.py in2.smi final file : /nfs/home/xiaobo/UCSF_scripts/2018-7-17-covalent_cys_wiki-tutorial/1-Custom-Ligand-Library-Generation/sulfonyl_chloride_Boc-acrylic-acid/final-sulfonyl_chloride_Boc-acrylic-acid.smi db2 file in /mnt/nfs/ex7/work/xiaobo/2017-6-30-sulfonyl_chloride_Boc-acrylic-acid/suc1 /mnt/nfs/ex7/work/xiaobo/2017-6-30-sulfonyl_chloride_Boc-acrylic-acid/suc2 ...
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2,5-Dichlorobenzenesulfonyl chloride, 98%, ACROS Organics 25g; Glass bottle Chemicals:Organic Compounds:Organohalogen compounds:Sulfonyl halides:Sulfonyl chlorides
China Sulfo Series catalog of P-Toluene Sulfonyl Chloride (PTSC), O/P-Toluene Sulfonamide (OPTSA) provided by China manufacturer - Heze Kingvolt Chemical Co., Ltd., page1.
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You are viewing an interactive 3D depiction of the molecule 2-deoxy-2-({[5-(dimethylamino)-1-naphthyl]sulfonyl}amino)-d-glucose (C18H24N2O7S) from the PQR.
Fumonisins, carcinogenic mycotoxins produced by various Fusarium species, occur naturally in maize and maize-based food products. They are hazards for animal and human health as they cause cancer in rodents and have been associated with oesophageal cancer and neural tube defects in humans. The most abundant naturally occurring fumonisins analogues in maize are fumonisin B1, B2 and B3 (FB1, FB2 and FB3). For analytical determination, they mostly require suitable extraction, clean-up and pre or post-column derivatization together with reversed-phase HPLC separation. o- Phthaldialdehyde (OPA) had been adopted as the most widely used derivatization reagent for fumonisins as they lack useful chromophores or fluorophores. Alternative derivatization reagents, naphthalene-2,3- dicarboxaldehyde (NDA) and dansyl chloride (DnS-Cl), were investigated in this study ...
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a) hydroxygallium naphthalocyaninetetrasulfonyl chloride (ClSO2)4NcGaOH 4Hydroxygallium naphthalocyaninetetrasulfonic acid 2 (374 mg; 0.334 mmol) from Example 1 was suspended in thionyl chloride (3 mL) containing a couple of drops of DMF. The reaction mixture was heated at 60-70° C. (bath) for 2 h under a slow stream of nitrogen, after which time the mixture became homogeneous and dark green in colour. After cooling to room temperature the resulting solution was diluted with chloroform (5 mL) and ether (50 mL) to precipitate the product. The supernatant liquid was decanted and the solid was suspended in ether (50 mL) and filtered off, washing with ether (3×10 mL). After drying under high vacuum the sulfonyl chloride 4 was obtained as a black solid (360 mg; 90%), that was insoluble in water, λmax (DMSO) 803 nm. The absorption spectrum of the sulfonyl chloride 4 is shown in FIG. 29.b) formation of the sulfonamide/ammonium sulfonic acid salt 5 from 2-(2-aminoethoxy)ethanolThe sulfonyl chloride 4 ...
3-Chloro-2-hydroxypropanesulfonic acid, sodium sa、Bis(benzene sulphonyl)imide、Bis-(sodium sulfopropyl)-disulfide、Diaminoarea Polymer
Copyright © 2013-2020 Hunan HuaTeng Pharmaceutical Co., Ltd. All right reserved Tel: +86 0731 89916275, +1 857-928-2050 E-mail: [email protected] Address: Lugu Business Plaza E1, Yuelu District, Changsha City, Hunan Province, P.R. China. ZIP Code: 410006. ...
0113] The selectivity of the anti-inflammatory effect is preferably achieved through the control of the counterion(s), if any, present on the sulphonyl groups or present on the multiple sulphonyl and carboxylic groups and the nature of the sulphonyl group. Generally speaking, it is believed that selection of, say, sodium counterions on --SO3- groups (i.e. a sulpho group in salt form) will favour concentration of sodium salts (e.g. sodium chloride) in the wound bed, whereas selection of, say, potassium counterions on --SO3- groups will favour concentration of potassium salts (e.g. potassium chloride) in the wound bed whereas selection of, say, calcium counterions on --SO3- groups will favour concentration of calcium salts (e.g. calcium chloride) in the wound bed. For example, we believe that it will be advantageous for the molar ratio of sodium ions to potassium ions associated in the hydrogel composition (or sodium ions to other more weakly hydrated cations according to the Hofmeister series of ...
The probes in this section bear some structural resemblance to natural lipids. Included in this section are our fluorescent fatty acid analogs, as well as phospholipids wherein one or both fatty acid esters are replaced by fluorescent fatty acid esters.
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BMS-833923, also known as XL-139, is an orally bioavailable small-molecule SMO (Smoothened) inhibitor with potential antineoplastic activity. SMO antagonist BMS-833923 inhibits the sonic hedgehog (SHH) pathway protein SMO, which may result in a suppression of the SHH signaling pathway. SMO is a G-protein coupled receptor that lies just downstream of the SHH ligand cell surface receptor Patched-1 in the SHH pathway; in the absence of ligand Patched-1 inhibits SMO and ligand binding to Patched-1 results in increased levels of SMO. The SHH signaling pathway plays an important role in cellular growth, differentiation and repair; constitutive activation of this pathway is associated with uncontrolled cellular proliferation and has been observed in a variety of cancers
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CIMmultus™ stands for: Convective mass transfer, reduced backpressure, and minimal sample clogging Fast product isolation and concentration of diluted
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... dansyl compounds MeSH D04.615.638.400 --- lovastatin MeSH D04.615.638.400.900 --- simvastatin MeSH D04.615.638.472 --- ...
This compound may be prepared by reacting the corresponding sulfonic acid with excess phosphorus oxychloride (POCl3) at room ... Dansyl chloride may also be denoted DNSC. Likewise, a similar derivative, dansyl amide is known as DNSA. In addition, these ... I. Enhancement of the fluorescence intensity of dansyl amino acids and dansyl proteins in aqueous media and its application to ... Exotic derivatives may have very different extinction coefficients, but others, such as dansyl amide, are similar to dansyl ...
Zinpyr-1 (ZP1), a compound containing a dichlorofluorescein fluorescent compound bound to two 2-picolamine (DPA) species that ... When the Dansyl group DNS binds to a metal, it loses a sulfonamide hydrogen, causing fluorescence quenching via a PET or ... It consists of a Dansyl group bound to styrylquinoline and operates by the disruption of intra-molecular charge transfer upon ... Small molecule sensors for it include: ZX1, a compound comprizing a dipicolylamine (DPA) Zinc binding subunit that has greater ...
... dansyl compounds MeSH D02.886.590.263 --- dapsone MeSH D02.886.590.263.050 --- acedapsone MeSH D02.886.590.625 --- ... trialkyltin compounds MeSH D02.691.850.900.910 --- triethyltin compounds MeSH D02.691.850.900.950 --- trimethyltin compounds ... mustard compounds MeSH D02.455.526.728.468 --- mustard gas MeSH D02.455.526.728.650 --- nitrogen mustard compounds MeSH D02.455 ... bephenium compounds MeSH D02.092.877.096.333 --- bretylium compounds MeSH D02.092.877.096.333.150 --- bretylium tosylate MeSH ...
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon ... dansyl and prodan derivatives) Coumarin derivatives oxadiazole derivatives: pyridyloxazole, nitrobenzoxadiazole and ... small organic compounds, synthetic oligomers and polymers, and multi-component systems. fluorescent proteins GFP (green), YFP ( ...
Chemically, N-methylphenyethanolamine is an aromatic compound, an amine, and an alcohol. The amino-group makes this compound a ... chromatographic and high resolution mass spectrometric determination of β-hydroxyphenylethylamines in tissues as dansyl-acetyl ... Subsequently, this compound (still in the form of its racemate) was studied more thoroughly by Chen and co-workers, who ... A. M. Lands (1952). "The cardiovascular actions of 1-(3-aminophenyl)-2-aminoethanol and related compounds." J. Pharmacol. Exp. ...
Billroth Gottlieb, Hans (1936). "The Replacement of Chlorine by Fluorine in Organic Compounds". J. Am. Chem. Soc. 58 (3): 532- ... dansyl chloride and later aminopeptidases and carboxypeptidases) and other general methods for sequence determination (e.g., ... edition, CRC Press, Boca Raton, Florida, 2009, ISBN 978-1-4200-9084-0, Section 3, Physical Constants of Organic Compounds, p. 3 ...
At that time, compounds resembling the aromatic amino acids had been shown to possess appreciable fluorescence in the near ... His list of achievements includes: the synthesis and use of dansyl chloride as a probe of protein hydrodynamics; the extension ... The result of two years of effort was the still popular probe dimethylaminonaphthalene sulfonyl chloride or dansyl chloride. ...
DNA detection: the compound ethidium bromide, when free to change its conformation in solution, has very little fluorescence. ... examples include: Indole, Cascade Yellow, prodan, Dansyl, Dapoxyl, NBD, PyMPO, Pyrene and diethylaminocumarin. This change is ... Ethidium bromide's fluorescence is greatly enhanced when it binds to DNA, so this compound is very useful in visualising the ...
The compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The ... labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a ... Nonlimiting examples of such compounds include the following; dansyl chloride; fluoresceins, such as 3,6-dihydroxy-9- ... Nucleic Acid Labeling Compounds. The nucleic acid labeling compounds of the present invention are of the following structure; A ...
0 (Dansyl Compounds); 0 (Ligands); 0 (Micelles); 0 (Serum Albumin); 1093-96-5 (dansylsarcosine); H806S4B3NS (Oxyphenbutazone); ... A computation of the first hyperpolarizability of the compound indicates that the compound may be a good candidate as a NLO ... The secreted compound is most likely structurally different from 4OH-OPB, as in PBMC and WB cultures, the inhibition of ... We devised a high-throughput screen for compounds that kill Mtb when its replication has been halted by reactive nitrogen ...
Dansyl fluoride , C12H12FNO2S , CID 93175 - structure, chemical names, physical and chemical properties, classification, ...
Dansyl-L-methionine , C17H22N2O4S2 , CID 152202 - structure, chemical names, physical and chemical properties, classification, ...
Fluorescent compounds include fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, etc. ... a fluorescent compound, or a chemiluminescent compound. A number of ligands and anti-ligands are optionally used. Where a ... System and method for electrochemical detection of biological compounds. US8133671. 14 Jul 2008. 13 Mar 2012. Handylab, Inc.. ... Alternatively, any haptogenic or antigenic compound is used in combination with an antibody (see, e.g., Coligan (1991) Current ...
Fluorescent compounds include fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, etc. ... One family of compounds is 2,3-dihydro-1,4-phthalazinedione. The most popular compound is luminol, which is a 5-amino compound ... a fluorescent compound, or a chemiluminescent compound. A number of ligands and anti-ligands can be used. Where a ligand has a ... reaction of amine-containing compounds with ninhydrin) or non-covalently (e.g., reaction of a compound with a labeled antibody ...
d) Splitting-off of the Dansyl Compound. Having concluded the quality check, the dansyl compound can be abstracted again by ... dansyl: 350 nm). The emission of the fluorescence (dansyl: 510 nm) is then read by a suitablereader (fluorescence microscope or ... In the case of the above-mentioned dansyl dye andusing 1 M DBU in acetonitrile, this result is already obtained after 1 to 2 ... Fluorescent compounds are preferred, such as dansylethanol, fluorescein or pyrene. Examples of further reporter groups are ...
Among the compounds are those of formulae I and II: ##STR1## ... Methods of use of compounds and compounds for the treatment of ... N-Dansyl-L-Leu-L-Leu-DL-norleucinal; N-Ac-L-Phe-L-Leu-DL-norleucinal; (2SR)-N-Cbz-L-Leu-L-Phe-DL-cyclohexylalaninal; and (2SR)- ... Compounds are also provided herein. These compounds may be used in the methods. The compounds have formulae I or II as defined ... Derivatives of the compounds, such as salts of the compounds or prodrugs of the compounds may also be used in formulating ...
... dansyl acid); dansyl acid Get C12 H13 N O3 S = dsy = 5-(dimethylamino)-2-naphthalenesulfonic acid Get C12 H14 N O5 S 1- = fro ... Hetero compounds with composition C H N O S. Get C H5 N O S = osm = 1-(oxidosulfanyl)methanamine Get C10 H10 N2 O2 S = qms = n ... dansyl-l-lysine Get C18 H25 N3 O7 S = gso = l-gamma-glutamyl-s-((2s)-2-hydroxy-2-phenylethyl)-l- cysteinylglycine Get C18 H26 ... compound 4-d Get C27 H29 N O5 S = inu = n-(3-cyclopropyl(5,6,7,8,9,10-hexahydro-2-oxo-2h- cycloocta(b)pyran-3-yl)methyl) ...
The most popular compound is luminol, which is the 5-amino compound. Other members of the family include the 5-amino-6,7,8- ... dansyl phosphatidylethanolamine; N,N′-dioctadecyl oxacarbocyanine; N,N′-dihexyl oxacarbocyanine; merocyanine, 4-(3′pyrenyl) ... These compounds can be made to luminesce with alkaline hydrogen peroxide or calcium hypochlorite and base. Another family of ... Individual fluorescent compounds which have functionalities for linking or which can be modified to incorporate such ...
Fluorescent compounds include fluorescein and its derivatives, rhodamine and itsderivatives, dansyl, umbelliferone, etc. ... a fluorescent compound, or a chemiluminescent compound. A number of ligands and anti-ligands can be used. Where a ligand has a ... These new chemical compounds can be identified by their abilities to inhibit the proteolytic activity of NC-p65 and arrest the ... The polypeptides can also be modified by extending or decreasing the compounds amino acid sequence, e.g., by the addition or ...
Several best fit compounds were synthesized and evaluated by their inhibition of MLCP/32P-MLC dephosphorylation, which resulted ... Several best fit compounds were synthesized and evaluated by their inhibition of MLCP/32P-MLC dephosphorylation, which resulted ... The first series of compounds synthesized were hydrophobic modifications of the ester linkage (compounds 10a,b, 13a,b,d,f,I and ... Dansyl chloride (750 mg, 2.78 mmol) was added to a solution of 4-hydroxyacetophenone (379 mg, 2.78 mmol), TEA (1 mL) and DMAP ( ...
Fluorescent compounds include fluorescein and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, etc. ... a fluorescent compound, or a chemiluminescent compound. A number of ligands and anti-ligands can be used. Where a ligand has a ... The manner of linking a wide variety of compounds to various surfaces is well known and is amply illustrated in the literature. ... If covalent bonding between a compound and the surface is desired, the surface will usually be polyfunctional or be capable of ...
Reactive DESI-MS Imaging of Biological Tissues with Dicationic Ion-Pairing Compounds: Anal Chem Anal Chem. 87(6), 3286-93 ... Luminescent dansyl-based ionic liquids from amino acids and methylcarbonate onium salt precursors: synthesis and photobehaviour ... The use of dicationic ion-pairing compounds to enhance the ambient detection of surface lipids in positive ionization mode ...
Therefore, we studied the ability of compound 14 to modify lysozyme. As depicted in Scheme 5, the compound lacking the boronic ... Dansyl and nitrobenzofurazan fluorescent motifs were modified with ethylenediamine and ethanolamine, respectively, and each ... compounds 5 and 6 were immediately tested for their ability to conjugate with lysozyme. Gratifyingly, both compounds retained ... Reaction of EDA-FA (100 μM) with compound 5 (100 μM) in acetate buffer (50 mM, pH 7) to obtain 8 confirmed by HRMS. HEK (A) and ...
Dansyl-PEG-phenylboronic acid , Protein labeling reagent , CAS [N.A.] , Axon 2257 , Axon Ligand™ with >98% purity available ... Compound for bioluminescence imaging (BLI) €95.00 The purity of Axon Ligands™ is confirmed by HPLC-MS, 1H-NMR and/or ...
Fluorescent compounds include fluorescent and its derivatives, rhodamine and its derivatives, dansyl, umbelliferone, and the ... Exemplary fluorescent compounds include fluorescent, Texas Red, rhodamine and the like. Dipstick format diagnostics of the ... Any haptenic or antigenic compound can be used in combination with a suitably labelled antibody for this purpose. Exemplary ... The fluorescence can be intrinsic or inherent to the material composing the solid support, or fluorescent compounds can be ...
By employing a plurality of the compounds, where the compounds share a common energy absorber/donor component and common ... A wide variety of energy absorbers may be employed, such as fluorescein, BODIPY, DAPI, Indo-1, coumarin, dansyl, cyanine dyes, ... Fluorescent dye compounds, conjugates and uses thereof. WO1999039203A1 *. Feb 2, 1999. Aug 5, 1999. Amersham Pharmacia Biotech ... A compound according to claim 14, wherein said energy donor component is covalently bonded at the 5 backbone terminus and said ...
This compound may be prepared by reacting the corresponding sulfonic acid with excess phosphorus oxychloride (POCl3) at room ... Dansyl chloride may also be denoted DNSC. Likewise, a similar derivative, dansyl amide is known as DNSA. In addition, these ... I. Enhancement of the fluorescence intensity of dansyl amino acids and dansyl proteins in aqueous media and its application to ... Exotic derivatives may have very different extinction coefficients, but others, such as dansyl amide, are similar to dansyl ...
If compounds are not derivatized, then a choice must be made as to what ionization mode should be used for LC-MS work-positive ... For instance, dansyl chloride is well known as a fluorescent reagent that reacts with amines and phenols. However, this moiety ... However, if unknown compounds are involved, then not only the high-resolution MS spectra but also UV visible and NMR spectra ... Comparison of the LC-MS data by LC-MS using MZmine suggested a compound with MH+ 259. Extracts of the P450 154A1 bacteria (in ...
Fluorescent compounds include fluorescein and its derivatives, rhodamine and its derivatives, dansyl, u-mbelliferone, and so ... Compounds and methods for modulating expression ApoB US9107933B2 (en) 2009-03-16. 2015-08-18. Isis Pharmaceuticals, Inc.. ... Compounds and methods for modulating expression ApoB US9347061B2 (en) 2007-03-24. 2016-05-24. Genzyme Corporation. ... The agent for polymerization may be any compound or system which will function to accomplish the synthesis of primer extension ...
To meet the demands of todays research, its Compound Management Group (CMG) in Basel, Switzerland, has embarked on a Compound ... The ligand binding assay uses a dansyl glycine probe and BSA to introduce fluorescence measurement. If the dansyl glycine is ... Frequently, the compounds involved in our screening trials are in limited supply, and we could clearly see the potential of ... The first few weeks are spent performing a natural product extraction, which is followed by the synthesis of a compound library ...
... a compound having a thiol group such as cystamine, a compound having biotin such as N-biotinylaminoalkylamine, a compound ... dansyl, fluorescamine, coumalin, naphthylamine, or their derivatives; rare earth fluorescent dyes [a combination of a rare ... a compound having an amino group such as N-trifluoroacetylaminoalkylcarboxylic acid, a compound having biotin such as N- ... Specifically, such a material includes, for example, silica compounds such as glass, quartz, silicone, etc., and synthetic ...
1. A compound of formula (I) ##STR7## where R1 is alkyl, alkenyl, or alkynyl containing 1 to 6 carbon atoms; or an aromatic ... of dansyl chloride and stirred at room temperature for 24 hours. The reaction mixture was diluted at 0 C. with 30 mL of 2N HCl ... Compounds and methods for treatment and prevention of diseases. USRE40596 *. 6. Febr. 2007. 2. Dez. 2008. Novartis Ag. ... 5. A compound of claim 1 wherein R1 is 4-[[4-(dimethylamino)phenyl]aza]phenyl or a pharmaceutically acceptable salt thereof. ...
2A, the left mouse was dosed with compound 3 and the right mouse with compound 1. Compound 3 yielded minimal fluorescence in ... Indomethacin conjugates to dansyl, dabsyl, coumarin, fluorescein, and rhodamine-derived fluorophores exhibited promising COX-2 ... A single fluorescent compound was detected in all the extracts, which coeluted with a standard of compound 2 in multiple HPLC ... This compound displayed an identical mass spectrum to the unmetabolized parent molecule, compound 2 (Fig. 4A). The time courses ...
  • Synthesis and characterization of all compounds is described in Supplementary Data. (aacrjournals.org)
  • Microscopic analysis of subcellular localization of a small library of fluorescently labelled SS-like peptides identified dansyl (DNS) as the lead fluorophore for the subsequent synthesis of chimaeric iron chelator-peptides of either catechol (compounds 10 and 11) or hydroxypyridinone (compounds 13 and 14) type. (biochemj.org)
  • Specifically, a dietary compound that conjugates with GSH can induce GSH synthesis, increase GSH concentration, and improve protection by GSH-dependent detoxification pathways in hRPE. (arvojournals.org)
  • A broad class of naturally occurring and synthetic compounds is known to induce synthesis of GSH and/or GSH-dependent detoxification systems in mammalian cells. (arvojournals.org)
  • Nowadays, to practice the mixture-based library strategy, mixtures of multiple candidate ligands are routinely prepared via the pooling of individual compounds, parallel combinatorial synthesis (PCS) or the fractionation of crude extracts of natural products. (biomedcentral.com)
  • To examine the interactions of asymmetric analogs of the OP compounds within the steric confines of AChEs, the synthesis of phosphonothiolate enantiomers as anti-AChEs was conducted using a chiral auxiliary for separation. (umt.edu)
  • Several best fit compounds were synthesized and evaluated by their inhibition of MLCP/ 32 P-MLC dephosphorylation, which resulted in the identification of novel MLCP inhibitors. (frontiersin.org)
  • We designed and synthesized a number of thiazole compounds targeting the protein phosphatase 1C (PP1C) portion of MLCP and examined inhibition of MLCP using the enzyme prepared from pig aorta. (frontiersin.org)
  • Many compounds were synthesized and screened for COX-2 inhibition in vitro and in intact cells. (aacrjournals.org)
  • By performing inhibition experiments of brief duration at 4 degrees C, it was established that the inhibition of the anti-DNP response by nonimmunogenic compounds was due to competitive blockade of receptors, and not tolerance or receptor modulation. (ox.ac.uk)
  • The inhibition rates of chromophore-FPs against recombinant mouse AChE (rMAChE) and electric eel AChE (EEAChE) were determined via colorimetric assay and the dansyl containing FPs were demonstrated to be the most potent. (umt.edu)
  • Understanding of how short cationic antimicrobial peptides interact with human serum albumin is of importance for developing such compounds into the clinics. (biomedcentral.com)
  • It was thought that the lipophilic nature of the polycyclic compounds would increase the activity of the fluorescent moieties by facilitating increased blood brain barrier permeability and penetration through cell membranes. (nwu.ac.za)
  • Although none of the novel fluorescent polycyclic compounds were found to be more potent than 7-NI (IC50 = 0.11 11M), the indazole pentacyclorindecane (5), the coumarin-adamantane (7), the dansyl-adamantane (8), and the cyanoisoindole-adamantane (11) conjugates, exhibited IC5o values below 1 µM. (nwu.ac.za)
  • The pharmacological properties of halostachine have some similarity to those of these structurally-related compounds, and Halostachys caspica extracts have been included as a constituent of certain OTC dietary supplements, but halostachine has never been developed as a prescription drug. (wikipedia.org)
  • The selectivity and recognition mechanism of the imprinted polymer film were studied with compounds that are structurally related to the template. (nus.edu.sg)
  • Las fenetilamidas y las indolalquilaminas O-metiladas fueron visualizadas en placas de fase reversa de alta resolución (HP-TLC), por cromatografía líquida de alta resolución (HPLC) y por cromatografía gas-líquido (CGL) y, además, fueron identificadas por cromatografía gas-líquido acoplada con espectrometría de masas (CGL-EM). (bvsalud.org)
  • The optimum derivatisation conditions for various compounds were determined by HPLC. (lboro.ac.uk)
  • Gratifyingly, both compounds retained their ability to functionalize the protein, yielding the expected constructs in ammonium acetate buffer (50 mM, pH 7.0) at room temperature as confirmed by ESI-FTICR-MS ( Schemes 3 and 4 ). (rsc.org)
  • The results suggest that, as compared to the synthetic compounds, the biological apatite has considerable surface heterogeneity, either chemical (impurities) or structural suggesting a scaffold surface that is more conducive of protein adhesion and cell attachment. (chemweb.com)
  • Although selective uptake into macrophages or tumor cells expressing COX-2 has been shown in vitro for some compounds, such selectivity has not been rigorously shown in vivo and significant nonspecific binding has been observed ( 18 ). (aacrjournals.org)
  • The presence of the hydroxy-group on the benzylic C of the N-methylphenylethanolamine molecule creates a chiral center, so the compound exists in the form of two enantiomers, d- and l-N-methylphenylethanolamine, or as the racemic mixture, d,l- N-methylphenylethanolamine. (wikipedia.org)
  • The polymeric recognition thin film, imprinted with chiral dansyl-L-phenylalanine, was immobilised on a gold electrode modified with a photoactive precursor monolayer via a self-assembly process using photopolymerisation. (nus.edu.sg)
  • Experimental and epidemiological evidence on non-organ specific cancer preventive effect of Korean ginseng and identification of active compounds. (sinbio.net)
  • Removal of the pinacol group using a boronic acid resin under acidic conditions afforded the final fluorescent compounds 5 and 6 in moderate yields ( Scheme 2 ). (rsc.org)
  • In the biological studies it was observed that four adamantane fluorescent compounds (7, 8, 10, 11) exhibited a high degree of inhibitory activity against the NOS enzyme and NMDA receptor and blocked VGCC. (nwu.ac.za)
  • The fluorescent compounds were further able to scavange detrimental neurodegenerative free radicals. (nwu.ac.za)
  • However, it is important to state that tea contains less studied bioactive compounds, such as biogenic amines (BAs) which are nonvolatile amines formed by decarboxylation of amino acids. (hindawi.com)
  • The invention provides platinum-based probe compounds having the structure: ##STR1## wherein: Pt is a platinum atom, PROBE is a probe biomolecule for associating to a target biomolecule, M is a detectable marker moiety, and X and Y are stabilizing substituents. (rpxcorp.com)
  • Also provided are platinum-based labeling compounds having the structure: ##STR2## wherein: Pt is a platinum atom, M is a detectable marker moiety, A is a displaceable leaving group, and X and Y are stabilizing substituents. (rpxcorp.com)
  • Theoretical studies are performed on compounds 5 and 6 to understand the structural changes that take place at the imidazole moiety upon deselenation. (chemweb.com)
  • The polyamine/NMDA antagonist N1-dansyl-spermine has been shown to be neuroprotective in a global ischaemia model. (tcd.ie)
  • A variety of methods and apparatus for the detection of an analyte of interest in a fluid sample is provided which relies upon the interaction of a fluorophore and a chromophoric light absorbing compound for qualitative and quantitative results. (google.es)
  • Reaching such low levels of concentration of estrogenic compounds is a challenge, even using state-of-the-art analytical methods. (ac.be)
  • In analytical chemistry, ultraviolet-absorption spectroscopy (UV spectroscopy) is applied for determining the concentration of a UV-active compound in the gas phase or in solution. (mdpi.com)
  • Compounds exhibiting kinetics described by a one-compartment model have a log concentration-time profile that is monophasic in nature (see Fig. 1.5). (pharmacologicalsciences.us)
  • General principles of redox chemistry and previous studies showing that the compounds are more oxidized than predicted from their standard reduction potentials are reviewed. (emory.edu)
  • We devised a high-throughput screen for compounds that kill Mtb when its replication has been halted by reactive nitrogen intermediates (RNIs), acid, hypoxia, and a fatty acid carbon source. (bireme.br)
  • These include: igher levels of reproducibility igher levels of extract cleanliness Reduced sample and solvent requirements Increased sensitivity SOLA has significant advantages for the analyst when processing compounds in complex matrices, particularly in high-throughput bioanalytical and clinical laboratories where reduced failure rate, higher analysis speed, and lower sample/solvent requirements are critical. (docplayer.net)
  • These compounds could possibly be used as molecular probes in the development of high-throughput screening or competitive NOS displacement assays. (nwu.ac.za)
  • Subsequently, this compound (still in the form of its racemate) was studied more thoroughly by Chen and co-workers, who confirmed its pressor activity, but observed that it was about one-half as potent as phenylethanolamine after i.v. administration in a cat preparation: a total dose of 5 x 10−6 M (or ~ 1 mg of the HCl salt) caused a maximum rise in blood pressure of 26 mm Hg. (wikipedia.org)
  • This was made possible through a deliberate choice of fluorophores (4-amino-1,8-naphthalimide, dansyl, and perylene) that produce zero spectral overlap with the spiro form and considerable overlap for the merocyanine forms. (chalmers.se)
  • The preparation of mixtures of multiple candidate ligands via the pooling of individual compounds suffers from the cost and time required for synthesizing individual compounds, but the composition ratios of candidate ligands in these mixtures can be rigorously controlled. (biomedcentral.com)
  • An intermediate in the oxidation of I3P to 1 is 3-hydroxy-2,4-di(1 H -indol-3-yl)butanal (HRMS established the presence of a compound with the formula C 20 H 19 N 2 O 2 ). (pubmedcentralcanada.ca)
  • However, the compound described in example 2 of U.S. Patent 5,503,770 (having formula (6)) is substantially insoluble in water, and as a practical matter requires the presence of an organic solvent such as methanol in order to work in a liquid environment. (allindianpatents.com)
  • The same patterns of histofluorescence are observed in rat brain slices after the intravenous or intracerebroventricular injection of both dansyl drugs. (elsevier.com)
  • The same patterns are also observed in dansyl-propranolol and 9-aminoacridine-propranolol (9-AAPN) treated animals as well as in untreated control rats. (elsevier.com)
  • Mass Frontier 5.0135 (HighChem, Ltd.) software was used to confirm the identity and structure of each 136 compound based on observed fragmentation patterns. (highchem.com)