T-cell enhancement of the B-cell response to thymic-dependent antigens.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
A method to identify and enumerate cells that are synthesizing ANTIBODIES against ANTIGENS or HAPTENS conjugated to sheep RED BLOOD CELLS. The sheep red blood cells surrounding cells secreting antibody are lysed by added COMPLEMENT producing a clear zone of HEMOLYSIS. (From Illustrated Dictionary of Immunology, 3rd ed)
A membrane-bound tumor necrosis family member that is expressed on activated antigen-presenting cells such as B-LYMPHOCYTES and MACROPHAGES. It signals T-LYMPHOCYTES by binding the OX40 RECEPTOR.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
Antigens which may directly stimulate B lymphocytes without the cooperation of T lymphocytes.
The altered state of immunologic responsiveness resulting from initial contact with antigen, which enables the individual to produce antibodies more rapidly and in greater quantity in response to secondary antigenic stimulus.
Multiple protein bands serving as markers of specific ANTIBODIES and detected by ELECTROPHORESIS of CEREBROSPINAL FLUID or serum. The bands are most often seen during inflammatory or immune processes and are found in most patients with MULTIPLE SCLEROSIS.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
DNA sequences encoding the beta chain of the T-cell receptor. The genomic organization of the TcR beta genes is essentially the same in all species and is similar to the organization of Ig genes.
Complex mental function having four distinct phases: (1) memorizing or learning, (2) retention, (3) recall, and (4) recognition. Clinically, it is usually subdivided into immediate, recent, and remote memory.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that causes rotting, particularly of storage tissues, of a wide variety of plants and causes a vascular disease in CARROTS; and POTATO plants.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
The phenomenon of antibody-mediated target cell destruction by non-sensitized effector cells. The identity of the target cell varies, but it must possess surface IMMUNOGLOBULIN G whose Fc portion is intact. The effector cell is a "killer" cell possessing Fc receptors. It may be a lymphocyte lacking conventional B- or T-cell markers, or a monocyte, macrophage, or polynuclear leukocyte, depending on the identity of the target cell. The reaction is complement-independent.
Enhancement of viral infectivity caused by non-neutralizing antibodies. There are at least two mechanisms known to account for this: mediation by Fc receptors (RECEPTORS, FC) or by complement receptors (RECEPTORS, COMPLEMENT). Either the virus is complexed with antiviral IMMUNOGLOBULIN G and binds to Fc receptors, or virus is coated with antiviral IMMUNOGLOBULIN M and binds to complement receptors.
The phenomenon of target cell destruction by immunologically active effector cells. It may be brought about directly by sensitized T-lymphocytes or by lymphoid or myeloid "killer" cells, or it may be mediated by cytotoxic antibody, cytotoxic factor released by lymphoid cells, or complement.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Unstable isotopes of chromium that decay or disintegrate emitting radiation. Cr atoms with atomic weights of 46-49, 51, 55, and 56 are radioactive chromium isotopes.
Antibodies produced by a single clone of cells.
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. It is the most common cancer in children and accounts for the vast majority of all childhood leukemias.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Resistance or diminished response of a neoplasm to an antineoplastic agent in humans, animals, or cell or tissue cultures.
A very toxic anthracycline aminoglycoside antineoplastic isolated from Streptomyces peucetius and others, used in treatment of LEUKEMIA and other NEOPLASMS.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)
Family of spider MITES, in the superfamily Tetranychoidea, suborder Trombidiformes.
Former state in north central Germany. Formally abolished March 1, 1947. Kingdom established 1701.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
A type of irritant dermatitis localized to the area in contact with a diaper and occurring most often as a reaction to prolonged contact with urine, feces, or retained soap or detergent.
The grafting of skin in humans or animals from one site to another to replace a lost portion of the body surface skin.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Serum containing GAMMA-GLOBULINS which are antibodies for lymphocyte ANTIGENS. It is used both as a test for HISTOCOMPATIBILITY and therapeutically in TRANSPLANTATION.
An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.
Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Elements of limited time intervals, contributing to particular results or situations.
Regular course of eating and drinking adopted by a person or animal.
Sequential operating programs and data which instruct the functioning of a digital computer.
A condition in which the FORAMEN OVALE in the ATRIAL SEPTUM fails to close shortly after birth. This results in abnormal communications between the two upper chambers of the heart. An isolated patent ovale foramen without other structural heart defects is usually of no hemodynamic significance.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE, infecting B-cells in humans. It is thought to be the causative agent of INFECTIOUS MONONUCLEOSIS and is strongly associated with oral hairy leukoplakia (LEUKOPLAKIA, HAIRY;), BURKITT LYMPHOMA; and other malignancies.
Tumors or cancer of the NASOPHARYNX.
Infection with human herpesvirus 4 (HERPESVIRUS 4, HUMAN); which may facilitate the development of various lymphoproliferative disorders. These include BURKITT LYMPHOMA (African type), INFECTIOUS MONONUCLEOSIS, and oral hairy leukoplakia (LEUKOPLAKIA, HAIRY).
Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. At least six nuclear antigens have been identified.
Proteins associated with the inner surface of the lipid bilayer of the viral envelope. These proteins have been implicated in control of viral transcription and may possibly serve as the "glue" that binds the nucleocapsid to the appropriate membrane site during viral budding from the host cell.
Substances elaborated by viruses that have antigenic activity.

Monocyte-mediated antibody-dependent cellular cytotoxicity: a clinical test of monocyte function. (1/2292)

The lack of a simple, rapid, and quantitative test of the functional activity of the monocyte has hampered studies of the contribution of this cell type to host defense and human disease. This report describes an assay of antibody-dependent cellular cytotoxicity, which depends exclusively upon the monocyte as the effector cell and therefore provides a convenient test of monocyte function. In this system, mononuclear leukocytes (MNL) obtained by Ficoll-Hypaque separation of whole blood are cytotoxic for 51Cr-labeled human erythrocyte targets coated with anti-blood group antibody. Removal of phagocytic monocytes from the MNL by iron ingestion, followed by exposure to a magnetic field, completely abolishes all cytotoxic activity from the remaining MNL population. Similarly, in severely mono-cytopenic patients with aplastic anemia, cytotoxic effector activity is absent. In normals and less severely monocytopenic aplastic anemia patients, cytotoxicity correlates significantly (p less than 0.001) with monocyte number. Application of this monocyte-mediated antibody-dependent cellular cytotoxicity assay to the study of patients with the Wiskott-Aldrich syndrome has revealed defective monocyte cytotoxic activity in spite of normal monocyte numbers, suggesting that this test may be useful for the assessment of monocyte function in a variety of clinical situations.  (+info)

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (2/2292)

Interleukin-12 (IL-12) is a potent antitumor cytokine, which induces and enhances the activity of natural killer (NK) cells, lymphokine activated killer (LAK) cells and cytotoxic T lymphocytes (CTL). IL-12 also stimulates IFN-gamma production from both T cells and NK cells. In this study, we transfected methylcholanthrene-induced fibrosarcoma (MCA-D) with TNF gene and investigated the therapeutic effect of TNF gene-transduced cancer vaccine and whether the vaccination effect is enhanced by systemic administration of recombinant IL-12 (rIL-12), in a murine model. TNF gene-transduced cancer vaccine or systemic administration of rIL-12 showed slight or moderate inhibition of pre-established tumor. However, simultaneous application of the vaccine and rIL-12 resulted in complete eradication. The cytotoxicity of CTL against parental tumor cells was enhanced with the combination of the vaccine and rIL-12, and IFN-gamma production from spleen cells also increased synergistically. Our findings show that synergistic enhancement of CTL activity and IFN-gamma production could play an important role in the antitumor effect of combination therapy using TNF gene-transduced cancer vaccine and rIL-12.  (+info)

Identification of a subpopulation of lymphocytes in human peripheral blood cytotoxic to autologous fibroblasts. (3/2292)

A naturally occurring subpopulation of human peripheral blood lymphocytes is cytotoxic to autologous and/or allogeneic fibroblasts. The autocytotoxic lymphocytes have a receptor for the third component of complement and for aggregated gamma globulin, do not form rosettes with sheep red blood cells, and are not removed by passage through nylon. The autocytotoxic subpopulation is not present in the thymus and tonsils of normal children or in the peripheral blood of individuals with X-linked agammaglobulinemia. Fibroblast absorption experiments demonstrate that the autocytotoxic cells are "sensitized" to antigens expressed on allogeneic fibroblasts in addition to the antigens expressed on autologous cells. Some normal individuals have a second subpopulation of lymphocytes that may "regulate" the autocytotoxic cells. The relevance of these observations to the murine autocytotoxic cells is discussed.  (+info)

Alloreactivity and association of human natural killer cells with the major histocompatibility complex. (4/2292)

All NK cells potentially lytic for autologous cells but not expressing self-major histocompatibility complex (MHC)-reactive receptors could be eliminated by a negative selection mechanism during ontogeny. This idea is based on the existence of a NK cell subset expressing a specific inhibitory receptor for allogeneic MHC alleles. As ancestral haplotypes of the MHC appear to define identical MHC haplotypes in unrelated individuals, unrelated individuals having the same ancestral haplotype should also have the same NK-defined allospecificities that have been shown to map to the human MHC. To test this prediction, multiple cell lines from unrelated individuals having the same ancestral haplotypes were tested for the NK-defined allospecificities. It was found that cells having the same ancestral haplotypes do have the same NK-defined specificities. Furthermore, the NK-defined phenotype of cells that possess two different ancestral haplotypes can be predicted from the NK-defined phenotypes of unrelated cells that are homozygous for the ancestral haplotypes concerned. Although the group 1 and 2 NK-defined allospecificities can be explained to some extent by HLA-C alleles, evidence is presented that additional genes may modify the phenotype conferred by HLA-C.  (+info)

Enhanced adjuvant effect of granulocyte-macrophage colony-stimulating factor plus interleukin-12 compared with either alone in vaccine-induced tumor immunity. (5/2292)

Using the poorly immunogenic D5 murine melanoma, we examined the adjuvant effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-12 (IL-12) secretion by gene-modified tumor cells inoculated as a vaccine to prime tumor-draining lymph nodes (TDLNs). D5 transfectants that secreted IL-12 or GM-CSF alone were compared with a double transfectant that secreted equivalent amounts of both cytokines. TDLN cells harvested 9-10 days after subcutaneous tumor inoculation were cultured sequentially in anti-CD3 and IL-2 and assessed for antitumor reactivity against wild-type D5 tumor. The double transfectant-induced TDLN effector cells had greater cytotoxicity in a long-term assay than TDLN cells primed by single transfectants. In adoptive immunotherapy, the TDLN cells primed by the double transfectant were significantly better at mediating the regression of established tumors compared with the TDLN cells elicited by the single transfectants. Both IL-12 and GM-CSF had adjuvant effects in promoting tumor-reactive TDLN cells, but the combination was better than either alone. These observations suggest that the immunomodulation roles of IL-12 and GM-CSF are different and complementary.  (+info)

An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence. (6/2292)

Pyolysin (PLO), the hemolytic exotoxin expressed by Arcanobacterium (Actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. Insertional inactivation of the plo gene results in loss of expression of PLO with a concomitant loss in hemolytic activity. The plo mutant, PLO-1, has an approximately 1. 8-log10 reduction in the 50% infectious dose compared to that for wild-type A. pyogenes in a mouse intraperitoneal infection model. Studies involving cochallenge of wild-type and PLO-1 bacteria resulted in recovery of similar numbers of both strains, suggesting that PLO production is required for survival in vivo. Recombinant, His-tagged PLO (His-PLO) is cytotoxic for mouse peritoneal macrophages and J774 cells in a dose-dependent manner. Protection against challenge with A. pyogenes could be afforded by vaccination with formalin-inactivated His-PLO, suggesting that PLO is a host-protective antigen, as well as a virulence determinant.  (+info)

Synovial fluid transforming growth factor beta inhibits dendritic cell-T lymphocyte interactions in patients with chronic arthritis. (7/2292)

OBJECTIVE: To examine whether rheumatoid synovial fluid (SF) inhibits dendritic cell (DC) expression of the CD80 and CD86 costimulator molecules and contributes to SF T lymphocyte hyporesponsiveness. METHODS: Cell-free rheumatoid SF was tested for its effect on DC-stimulated autologous/allogeneic mixed lymphocyte reactions and for its effect on DC surface antigen expression, as assessed by flow cytometry. Blocking monoclonal antibodies were used to identify the SF cytokines that inhibited DC-T lymphocyte interactions. RESULTS: Low concentrations of SF (2.5%) could inhibit DC-mediated autologous and allogeneic T lymphocyte proliferation. This inhibitory effect could be reversed by neutralizing transforming growth factor beta (TGFbeta) and interleukin-2 (IL-2), but not by IL-12, in the SF. Hyaluronic acid, IL-6, IL-10, and tumor necrosis factor alpha were not associated with SF inhibition. In vitro culture alone and crosslinking with the CD40 ligand up-regulated DC CD80/CD86 expression and costimulator function, and this was not affected by inclusion of SF. In the presence of SF, DC clustered with autologous T lymphocytes showed decreased CD80 and CD86 expression, and variable CD80/CD86 decreases were observed on DC clustered with allogeneic T lymphocytes. CONCLUSIONS: TGFbeta in SF appears to suppress T lymphocyte function, which may affect both signaling to DC and the induction of DC costimulator function.  (+info)

Skin reaction and antibody responses in guinea-pigs sensitized to human leukaemia cells or their nuclei in combination with Bacillus Calmette-Guerin. (8/2292)

Guinea-pigs sensitized by subcutaneous injection of chronic lymphatic leukaemia (CLL) cells combined with Bacillus Calmette-Guerin (BCG) displayed good skin reacitons 24 and 48 h after challenge with CLL cells. Equally good responses were also demonstrated using nuclei from the leukaemic cells in combination with BCG. These reactions were significantly greater than those produced in the same manner but without BCG. Sera form the animals were examined for the presence of antibodies against CLL cells by cytotoxicity and immunofluorescence techniques. Only samples from guinea-pigs innoculated with CLL cells were found to contain significant antibodies. Histological examination showed that whereas leukaemic cells persisted at the sensitizing injection site leukaemic cell nuclei could not be visualized. It is suggested that because leukaemic cell nuclei in combination with BCG are able to induce good skin reactivity without provoking a vigorous humoral antibody response they may have possible advantages over leukaemic cells when used for immunotherapy.  (+info)

Waterfield, J D. and Waterfield, E M., Lymphocyte-mediated cytotoxicity against tumor cells. II. Characteristics and tissue distribution of concanavalin a-activated cytotoxic effector cells. (1976). Subject Strain Bibliography 1976. 2858 ...
Lill, P H. and Fortner, G W., Identification and cytotoxic reactivity of inflammatory cells recovered from progressing or regressing syngeneic uv-induced murine tumors. (1978). Subject Strain Bibliography 1978. 991 ...
Gentaur molecular products has all kinds of products like :search , Neuromi \ Total Cytotoxicity Test Kit, 250 Tests. \ CT17004 for more molecular products just contact us
TY - JOUR. T1 - Dietary fat influences on murine melanoma growth and lymphocyte-mediated cytotoxicity. AU - Erickson, Kent L. PY - 1984. Y1 - 1984. N2 - The effects of fat concentration and saturation on the growth of a B16 melanoma and lymphocyte-mediated cytotoxicity against the cells were studied with the use of inbred C57BL/6J and C3H/HeJ mice subjected to dietary manipulation before and after tumor transplantation. The tumor latency for mice initially given injections of 5 x 106 syngeneic B16 melanoma cells was significantly less for those mice fed at 20% fat concentration than those fed only the essential fatty acid (EFA) diet. When mice were given injections of 106 melanoma cells, the initiation time required for visible tumor growth in mice receiving the polyunsaturated fat (PUF) diet was significantly less than that in mice receiving the saturated fat (SF) diet. Cytolysis mediated by lymphocytes from diet-manipulated mice toward allogeneic B16 melanoma cells was greater for those mice ...
Looking for antibody-dependent cell-mediated cytotoxicity? Find out information about antibody-dependent cell-mediated cytotoxicity. An immunologic response in which an immunologic effector cell binds to a target cell coated with antibodies, triggering a series of metabolic events that... Explanation of antibody-dependent cell-mediated cytotoxicity
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Authors: Matsuoka, Atsuko , Önfelt, Agneta , Matsuda, Yoshie , Nakaoka, Ryusuke , Haishima, Yuji , Yudasaka, Masako , Iijima, Sumio , Tsuchiya, Toshie Article Type: Research Article Abstract: To evaluate the role of particle size in cytotoxicity tests of nanomaterials (NMs), we exposed Chinese hamster cells to polystyrene (PS) spheres with defined diameters ranging from 0.1 to 9.2 μm. We found that the 4.45-μm PS particles were most cytotoxic while sizes 0.1 and 0.2 μm showed no cytotoxicity up to 1000 μg/ml. In the chromosome aberration test, the 4.45-μm PS particles induced polyploidy in a mass concentration-dependent manner in 24- and 48-h treatments. The 5.26-μm PS particles induced polyploidy only at 1000 μg/ml for 48 h. Next, we performed the cytotoxicity test with as-grown single walled carbon nanohorns …(NHas). These were suspended in DMSO and then transferred into the culture medium followed by sonication. Six suspensions differently sonicated showed the same apparent ...
Benefits of the system. One of the major benefits over the Ames test is that the entire DNA content of the cell functions as a target for the genotoxin to display its effect. In contrast, the Ames test monitors mutations only in genes related to histidine synthesis. This results in a significantly lower sample concentrations (up to 1000 fold) required for the Vitotox test.. Simultaneously cytotoxicity assay. An important control for the system is a cytotoxicity test performed together with each sample to ensure that a positive signal in the genotoxicity test is not due to a nonspecific enhancement in luminescence and that a negative response is not due to toxicity of the test compound resulting in cell death. Performing Vitotox assay. The test is very simple and cost efficient to perform. Compounds are pipetted into 96-well or 386-well microplates in a dilution series along with blanks, positive control samples, and with or without S9 metabolic extract. The cultured test bacteria are added and ...
Supplied as five vials (5 µL per vial, 1 mM in DMSO). Each vial sufficient for 100-200 live cell imaging cytotoxicity tests in 96-well format. Incucyte® Cytotox Reagents are highly sensitive cyanine nucleic acid dyes ideally suited to a simple mix-and-read, real-time quantification of cell death. Addition of the Incucy
Supplied as five vials (5 µL per vial, 1 mM in DMSO). Each vial sufficient for 100-200 live cell imaging cytotoxicity tests in 96-well format. Incucyte® Cytotox Reagents are highly sensitive cyanine nucleic acid dyes ideally suited to a simple mix-and-read, real-time quantification of cell death. Addition of the Incucy
Supplied as five vials (5 µL per vial, 1 mM in DMSO). Each vial sufficient for 100-200 live cell imaging cytotoxicity tests in 96-well format. Incucyte® Cytotox Reagents are highly sensitive cyanine nucleic acid dyes ideally suited to a simple mix-and-read, real-time quantification of cell death. Addition of the Incucy
Cytotoxicity assay on Müller cell. A: The percentage of trypan blue positive cells (y-axis) was plotted against the concentration of the test compounds used (x
This article shows that the CellTox™ Green Cytotoxicity Assay can be multiplexed with a luminescent, fluorescent or colorimetric cell viability assay, generating more data from each sample.
The MultiTox-Glo Multiplex Cytotoxicity Assay is a fluorescent and luminescent assay that measures the relative number of live and dead cells in a population.
اطلاعات مندرج در این پایگاه فقط جهت مطالعه کاربران با رعایت شرایط اعلام شده است. نسخه‌برداری و بازنشر اطلاعات به هر روش، در هر نوع رسانه و با هر هدفی ممنوع و پیگرد قانونی دارد. ...
In this MS thesis, cytotoxicity tests of various nanomaterials, which have commercial applications and involve a contact with human body, were performed at different conditions. The nanomaterials used for the experiments included pristine 100 ply carbon nanowire, graphene nanoflakes, multiwall carbon nanotubes, nanoclay and C60 (buckyball or fullerene). The nanomaterials cytotoxicities were calculated and compared to each other using human and mice fibroblast cancerous cells (3T3 and L929 resp.). The in vitro MTT Assay was used as the testing method because of its simplicity and reliability. The results were analyzed by means of a spectrophotometer at 590 nm wavelength. From the optical density studies, the viabilities were calculated and the toxicities of those nanomaterials were compared. Pristine 100 ply carbon nanowire was the most viable nanomaterial with the average viability value of 86.9%. The effect of dilution on carbon nanowire was negligible which may have be due to its single ...
Cytotoxicity tests for high-throughput drug discovery.: Despite theoretical obstacles associated with performing cell-based assays in high-density formats (micr
In the present study, an attempt was made to characterize the immunomodulating abilities of the cytostatic drugs cydophosphamide, ifosfamide, vinblastine, vincristine, procarbazine, dacarbazine, 6-mercaptopurine, methotrexate, 5-f/uor-uracil and adriamycine in a defined experimental model. Varying combinations of drug plus transplantation alloantigen, (C3H-lymphocytes) were injected into Balb/c mice at different time intervals in vivo. The resulting T-effector cell reactivity was determined in vitro with the microcytotoxicity assay on day + 5 for primary (r) and day + 7 for secondary (2°) sensitized mice. According to the type of drug (alkylating agent vs. vinca alkaloid vs. antimetabolite vs. cytostatic antibiotic), the dosage (20% LD50 vs. 60% LD50), the state of sensitization (r vs. 2° sensitized recipients), and the time of drug application in relation to the antigen treatment on day 0 (in varying steps from day -6 to day +4), so-called pharmaconantigen- variation-effects (PA VE) were ...
ABSTRACT The present study aims to investigate the cytotoxic effect of the major aflatoxins (B1, B2, G2 and G2) and also aflatoxin combination, using a simple, rapid and cheap cytotoxicity test like MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay in three in vitro models (human umbilical vein endothelial cells [HUVEC], human lung fibroblasts [HFL] and A2780 cell line) and to extrapolate the data to in vivo situation using a prediction model. A difference in cell sensitivity has been observed for B1 and B1 + B2, in the following order A2789 > HFL > HUVEC, while for B2, G1, G2, Mix (B1 + B2 + G1 + G2) the order was HFL > A2789 > HUVEC when comparing the IC50 (half maximal inhibitory concentration) values. We confirm that in vitro cytotoxicity test MTT assay is able to predict in vivo toxicity, at least for aflatoxins using the prediction model. The values of LD50 (lethal dose 50%) calculated from experiments are different for each cell line. This fact may indicate that ...
MSCR provides comprehensive in vitro toxicology and safety pharmacology services to assess safety profiles early in the discovery process. We offer reliable, high-throughput models of multiplexed toxicity testing to provide quick and cost-effective screening solutions that are suitable for early compound liability assessment.
Supplied as five vials (5 µL per vial, 1 mM in DMSO). Each vial sufficient for 100-200 live cell imaging cytotoxicity tests in 96-well format. Incucyte® Cytotox Reagents are highly sensitive cyanine nucleic acid dyes ideally suited to a simple mix-and-read, real-time quantification of cell death. Addition of the Incucy
Quickly and accurately calculate the cell cytotoxicity from absorbance data obtained in lab assays, improving overall lab performance.
TY - JOUR. T1 - Cellular immune defects in patients with melanoma involving interleukin-2-activated lymphocyte cytotoxicity and a serum suppressor factor. AU - Balch, C. M.. AU - Itoh, K.. AU - Tilden, A. B.. N1 - Copyright: Copyright 2004 Elsevier B.V., All rights reserved.. PY - 1985. Y1 - 1985. N2 - Interleukin-2 (IL-2) can stimulate human blood lymphocytes to acquire tumor cytotoxicity, designated as lymphokine-activated killer (LAK) cytotoxicity. In 29 of 33 patients with melanoma, LAK cytotoxicity could be induced with recombinant IL-2 against fresh (noncultured) autologous and/or allogeneic melanoma cells. There was an excellent correlation of LAK cytotoxicity with the clinical stage of disease when the cells were incubated with medium containing IL-2 plus 10% fetal calf serum (p = 0.003). The 14 patients with localized melanomas (group I) had the same level of specific lysis as did 16 normal controls (24% versus 25%). Eleven patients with resectable regional or distant metastases (group ...
Paul Ichiro Terasaki (Japanese: 寺崎一郎, September 10, 1929 - January 25, 2016) was an American scientist in the field of human organ transplant technology, and professor emeritus of surgery at UCLA School of Medicine. He spent three high school years during World War II interned with his family and other Japanese Americans in the Gila River War Relocation Center. Later he earned his bachelors, masters, and doctorate in zoology all from UCLA and was appointed to the medical school faculty. In 1964, Terasaki developed the microcytotoxicity test, a tissue-typing test for organ transplant donors and recipients that required only 1 microliter each of antisera used to identify human leukocyte antigens (HLA). The test was adopted as the international standard for tissue typing. He has focused on study of the humoral theory of transplant rejection, which states that antibodies cause allograft rejection. He and his corporation, One Lambda, have played a central role in the development of tissue ...
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Compared to GEA, AEA appears to preserve perioperative NKCC. This effect may be related to an attenuated stress hormone response associated with AEA. Cancer patients may have improved killing of embolized tumor cells during surgery performed under AEA.
Biological evaluation performed on the active substance confirmed no skin irritation or sensitization potential. A slight toxicity potential for the material extract has been revealed under the test conditions in cytotoxicity test; this potential does not manifest itself in the overall local skin reaction in vivo, and can in contrast act as an initiator of healing processes. Very low systemic toxicity (LD50 greater than 5 g per kg body weight for small animals) and no adverse effects at multiple overdosing are observed, as compared with typical dosage of less than 1 g per ...
Hi, I am looking for a non radioactive CTL assay. I am currently using a method to detect Serine Esterase which is released during CTL mediated lysis. I am facing a bit of a problem using this assay for Tcell clones and am thus interested in finding out a method that uses a cytoplasmic marker which will only be released from target cells on lysis. I have heard of a LDH release assay (Lactate Dehydrogenase) can any one help me with a protocol for this assay? Thanking you in advance. Shyam shyam at nii.ernet.in SRF, Nationl Institute of Immunology New Delhi India P.S. : Please send the reply directly to this Email address given above, The guest account at IMtech form where this message wasposted is several hundred KM away and can only be accessed irreat irregular intervals. Thanx again ...
Research have got proven that IL-15 and IL-2 showed contrasting assignments during CIK cells planning. on the reflection of a pool of growth cytotoxic genetics in CIK cells. The DEMs between CIKIL-15 and CIKIL-2 may contribute to enhanced tumor cytotoxic capacity of CIKIL-2. Significantly, we found that repressed miR-193a-5p might regulate the expressions of inhibitory receptor KLRD1. The total results of the validation assay possess shown that KLRD1 were upregulated in CIK cells. Our results have got supplied brand-new ideas into systems of CIK cells growth and creation cytotoxic function, and shed light on their basic safety for scientific trial. Amazing technological developments have got been converted into better methods to prevent, identify, diagnose and deal with tumor during the previous five years1. Today, people are making it through much longer after their malignancy offers been diagnosed credited to these impressive improvement. Several therapeutics against malignancy possess ...
Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118). 1000 tests. Quantify as few as 300 cells. Higher sensitivity than MTT and XTT. HTP ready.
MTT Cell Proliferation and Cytotoxicity Assay Kit, For quantitation of viable cell number in proliferation and cytotoxicity assays
Petricevic B, Laengle J, Singer J, Sachet M, Singer J, Steger G, Bartsch R, Jensen-Jarolim E, Bergmann M. 2013. Trastuzumab mediates antibody-dependent cell-mediated cytotoxicity and phagocytosis to the same extent in both adjuvant and metastatic HER2/neu breast cancer patients. Journal of Translational Medicine. 11, 307 ...
Petricevic B, Laengle J, Singer J, Sachet M, Singer J, Steger G, Bartsch R, Jensen-Jarolim E, Bergmann M. 2013. Trastuzumab mediates antibody-dependent cell-mediated cytotoxicity and phagocytosis to the same extent in both adjuvant and metastatic HER2/neu breast cancer patients. Journal of Translational Medicine. 11, 307 ...
Large variations in MTT - posted in MTT, Proliferation and Cytotoxicity Assay: Control (Sample 1) 0.331 , 0.333 (Sample 2)0.457, 0.454 (Sample 3)0.450, 0.451 Treatment 1 (Sample 1) 0.390 , 0.374 (Sample 2)0.439, 0.458 (Sample 3)0.441, 0.444 Treatment 2 (Sample 1) 0.418 , 0.424 (Sample 2)0.446, 0.471 (Sample 3)0.406, 0.436 + Control (Sample 1) 0.428 , 0.454 (Sample 2)0.429, 0.432 (Sample 3)0.441, 0.427 My cells are adherent cancer cells, seeded in 24-wells at 2500 cells/well and harvested...
The antibody-dependent cell-mediated cytotoxicity (ADCC), also referred to as antibody-dependent cellular cytotoxicity, is a mechanism of cell-mediated immune defense whereby an effector cell of the immune system actively lyses a target cell, whose membrane-surface antigens have been bound by specific antibodies. It is one of the mechanisms through which antibodies, as part of the humoral immune response, can act to limit and contain infection. ADCC is independent of the immune complement system that also lyses targets but does not require any other cell. ADCC requires an effector cell which classically is known to be natural killer (NK) cells that typically interact with IgG antibodies. However, macrophages, neutrophils and eosinophils can also mediate ADCC, such as eosinophils killing certain parasitic worms known as helminths via IgE antibodies. ADCC is part of the adaptive immune response due to its dependence on a prior antibody response. The coating of target cells with antibodies is ...
TY - JOUR. T1 - HIV-1 gp120-Specific Antibody-Dependent Cell-Mediated Cytotoxicity Correlates with Rate of Disease Progression. AU - Baum, Linda L.. AU - Cassutt, Kelly J.. AU - Knigge, Kevin. AU - Khattri, Roli. AU - Margolick, Joseph. AU - Rinaldo, Charles. AU - Kleeberger, Cynthia A.. AU - Nishanian, Parunag. AU - Henrard, Denis R.. AU - Phair, John. PY - 1996/9/1. Y1 - 1996/9/1. N2 - The Ab-dependent cell-mediated cytotoxicity (ADCC) activity of anti-gp120 Abs in serum from four groups of HIV-1-positive individuals in the Multicenter AIDS Cohort Study was evaluated at several time points over a 10-yr period. HIV-1-positive individuals who progressed to AIDS within 3 yr of seroconversion (rapid progressors) were compared with seroconverters who did not progress to AIDS within 6 yr (nonrapid progressors) and individuals who were seropositive when they entered the study and did not progress to AIDS within 9-10 yr (nonprogressors). At the visit closest to AIDS, rapid progressors had ...
With the aim of improved prediction of human skin irritation, cytotoxicity tests were conducted using a reconstituted human dermal model (Skin2TM cultured 3-Dimensional Skin Model, ZK1300: 3-D model). A range of water-insoluble substances, namely fatty acids, fatty alcohols and hydrocarbons, were tested with MTT(3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl tetrazolium bromide) reduction, LDH (lactate dehydrogenase) release and prostaglandin E2(PGE2) release using as cytotoxicity markers. Results for these parameters were compared with those obtained from a cytotoxicity test (MTT reduction assay) using cultured monolayers of human dermal fibroblasts (NB1RGB), from a rabbit primary skin irritation test and from human patch testing ...
Modern subunit vaccines stimulate the B cell immune system and induce neutralizing Abs. However, neutralizing Abs cannot be effective against pathogens that hide intracellularly (e.g., Plasmodium malariae), or that permanently change the antigenic nature of their surface structures (antigenic drift), as is the case with seasonal influenza or HIV. Therefore, there is a need for vaccines utilizing the second powerful arm of the adaptive immune system, the cytotoxic defense (1). In this type of immunity, CD8+ T cells specifically recognize peptide fragments derived from (often conserved) pathogen proteins that are presented on the surface of infected cells in the context of MHC I. As a result, the CD8+ T cells become activated, acquire cytotoxic capacity, and eliminate these infected cells in a highly specific fashion.. Apart from prophylactic vaccination, a therapeutic vaccination employing the cytotoxic potential of the immune system is also needed due to the limitations of current therapies ...
Consecutive serum samples were obtained from patients with syphilis before and on three occasions after treatment. The sera contained immunosuppresive factors associated with the immunoglobulin fraction, which could depress the natural killer cell activity of healthy controls. There was no evidence that allogeneic or lymphocytotoxic antibodies played a role in immuno-suppression, which could be reproduced with both soluble and insoluble antigen-IgG-antibody complexes.. ...
HIV-1-infected cells presenting envelope glycoproteins (Env) in the CD4-bound conformation on their surface are preferentially targeted by antibody-dependent cellular-mediated cytotoxicity (ADCC). HIV-1 has evolved sophisticated mechanisms to avoid the exposure of Env ADCC epitopes by downregulating CD4 and by limiting the overall amount of Env on the cell surface. In HIV-1, substitution of large residues such as histidine or tryptophan for serine 375 (S375H/W) in the gp120 Phe 43 cavity, where Phe 43 of CD4 contacts gp120, results in the spontaneous sampling of an Env conformation closer to the CD4-bound state. While residue S375 is well conserved in the majority of group M HIV-1 isolates, CRF01_AE strains have a naturally occurring histidine at this position (H375). Interestingly, CRF01_AE is the predominant circulating strain in Thailand, where the RV144 trial took place. In this trial, which resulted in a modest degree of protection, ADCC responses were identified as being part of the ...
Assay Kits , Cell Proliferation and Cytotoxicity Kits , Sensolyte Cell Cytotoxicity Assay Kit-Larger size; The damage of cell membrane leads to release of cytoplasmic enzymes. The measurement of released cytoplasmic lactate dehydrogenase (LDH) is a well-accepted assay to estimate cell membrane integrity and quantify cytotoxicity. The SensoLyte Cell Cytotoxicity Assay Kit uses resazurin as a sensitive fluorogenic indicator (Ex/Em=560 nm/590 nm upon conversion) to measure LDH activity. The assay can be performed in a mixed population of damaged and viable cells, but only measure the LDH released from damaged cells. The cytoplasmic LDH in living cells produces little signals under assay condition. There is no need of extra steps to separate living cells and supernatant. The fluorescent signal is proportional to the number of damaged cells (up to 2.5X104 cell, r2>0.95) with the detection limit reaching 100 dead cells. The kit is suitable for high throughput screening of
Chung Y.S., Spradbrow P.B. and Wilson B.E. (1977) Cytotoxic antibody in the serum of cattle with squamous cell carcinoma. Research in Veterinary Science, 22 2: 263-264. ...
p{text-align: justify;} What is activated autologous lymphocyte therapy? Activated autologous lymphocyte therapy was developed by Dr. Teruaki Sekine, Chairman of the Board of Directors of our company. This is immune cell therapy
Supplementary MaterialsSupplementary materials 1 (PDF 538?kb) 262_2017_2011_MOESM1_ESM. and M2?M?s became vunerable to V2+ T cell cytotoxicity. V2+ T cells portrayed and degranulated in response to ZA-treated M perforin? s seeing that shown by mobilisation of Compact disc107b and Compact disc107a ...
In this application note, we examine how Molecular Devices SpectraMax i3x Multi- Mode Microplate Reader, in combination with CellTiter-Glo luminescent assays for cell viability and cytotoxicity.
BioTek 应用手册, 12/13 2017, An Image-Based Method to Detect and Quantify T Cell Mediated Cytotoxicity of 2D and 3D Target Cell Models
BACKGROUND: The anti-HLA antibody can be detected using either a complement-dependent lymphocytotoxicity (CDC) assay or a flow cytometry crossmatch (FCXM) in renal transplantation. Discordant results are often obtained because the two methods detect different reaction phases between the donor lymphocytes and the recipient sera. This study was intended to confirm that antibody binding and cytotoxicity to the lymphocytes can be detected simultaneously in a single FCXM assay, cytotoxic FCXM.. METHODS: In the cytotoxic FCXM, the antibody binding to the lymphocytes was measured using anti-IgG-FITC, and the cytotoxicity using 7-aminoactinomycin D (7-AAD) after adding complement. For an evaluation of two test parameters, the cytotoxicity test moiety (dead-cell percentage) was compared with the anti-human globulin (AHG)-CDC, and the antibody-binding test moiety (sample/control fluorescence ratio) with the conventional FCXM in 77 positive and 30 negative crossmatches.. RESULTS: In the cytotoxic FCXM, ...
Large-scaled composite hydrogels (several. centimeters) have been prepared for use in biomedical applications such as cartilage and bone. However, few preparation methods of nanocomposites of PVA and Hap have been reported. In this study, the nano-, microparticles of PVA, HAp and DNA were obtained by using high hydrostatic pressure technology. It is thought that this is achieved by the pressure-induced Inhibitors,research,lifescience,medical quick formation of PVA particles that could incorporate secondary and third substrates, such as DNA and HAp, without phase separation [15, 31]. 3.2. Cytotoxicity Test Figure 4 shows the result of the cytotoxicity test of PVA/DNA and PVA/HAp/DNA complexes. Inhibitors,research,lifescience,medical The high viability of COS-7 cells incubated with them is shown, irrespective of the concentration of PVA and HAp. PVA and HAp are biocompatible materials [32, 33]. The PVA/DNA complex is nontoxic. because of the composite formation of PVA and DNA via hydrogen bonding ...
To establish if a correlation exists between the expansion of GP33-specific cells observed by flow cytometry and GP33-specific cytotoxicity, direct CTL assays were carried out using spleen cells from the mice described above. CTL activity mediated by antigen-specific CD8+ cells is usually difficult to measure at day 3 after infection since LCMV induces, at this early time point, strong NK cell activity that lyses both antigen-pulsed and unpulsed target cells. To circumvent this problem, direct CTL assays were carried out by adding a twofold excess of unlabeled NK-sensitive target cells (YAC-1 cells) to assay wells as cold-target competitors. Inclusion of cold YAC-1 cells abrogated lysis of chromium-labeled MC57 target cells by NK cells from the spleens of mice that had been injected 48 h previously with poly-IC (data not shown). No inhibition of antigen-specific CTLs was observed when YAC-1 cells were included as cold-target competitors in a direct CTL assay using spleen cells from mice that had ...
Author(s): Ebina, N; Gallardo, D; Shau, H; Golub, SH | Abstract: Interleukin 4 (IL-4) suppresses the interleukin 2 (IL-2) induced lymphokine-activated killer (LAK) cell development from human peripheral blood mononuclear cells (PBMC). Suppression is observed at high (1,000 U ml-1) as well as low (10 U ml-1) concentrations of IL-2. IL-4 needs to be present at the beginning of the IL-2 culture to exert the suppressive effect. IL-4 also inhibits the development of CD25 (Tac) antigen on the PBMC cultured in IL-2. Interleukin 1 (IL-1) can reverse the suppressive effect of IL-4 on LAK induction when added at the early phase of the IL-2 culture. IL-1 enhances IL-2 induced LAK development, which may partially explain the reversion of IL-4 inhibition by IL-1. IL-1 also reverses the inhibitory effect of IL-4 on the development of CD25 antigen expression, although IL-1 alone does not enhance the induction of CD25 expression in PBMC cultured by IL-2. Furthermore, IL-4 suppresses IL-2 induced IL-1 production in PBMC
Lymphocytes T Cytotoxiques 0 questions Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from natural killer cells (KILLER CELLS, NATURAL) and from KILLER CELLS mediating antibody-dependent cell cytotoxicity. There are two effector phenotypes: TC1 and TC2. ...
Abstract: Cancer is one of the leading causes of death worldwide, therefore struggles to find more effective treatment and prevention is needed. Several studies has been performed using natural ingredients, one of which is Temu Kunci (B. pandurata). Temu Kunci extract contains flavonoid pinostrobin that has been showed as having cytotoxicity effects. Cytotoxicity tests of pinostrobin have been performed on several tumor cell lines, but its cytotoxicity effect on HeLa cell line has never been reported. ...
These are all influencing factors of the interaction between the mAb and the antigen (or mAb and effector molecules) and thus should be evaluated at an early stage of biosimilar development prior to final lead molecule identification.. In particular, the carbohydrate profile is important for the evaluation of complex effector functions, including Antibody-dependent Cell-mediated Cytotoxicity (ADCC) and Complement-dependent cytotoxicity (CDC).. The glycosylation profile of a mAb can vary in terms of:. ...
Knowledge of the interactions between MHC-unrestricted cytotoxic effector cells and solid tumour cells is essential for introducing more effective NK cell-based immunotherapy protocols into clinical practise. Here, to begin to obtain an overview of the possible universe of molecules that could be in …
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of NTU Repository with Academic Hub to form NTU Scholars.. ...
BioTek Arquivos de Exemplos, 08-Mar-13, DELFIA ADCC Cytotoxicity Assay reading parameters and % Maximum Cytotoxicity calculations for EC50 value
A persons white blood cells. Lymphocytes have a number of roles in the immune system, including the production of antibodies and other substances that fight infection and disease ...
IFN-omega1 (Interferon omega) exhibits antiproliferative effects and stimulates Natural Killer cell activity. It is 75% homologous to IFN-alpha.
This vaccine is currently being tested in animals and hopefully will be an effective contraceptive for humans. Normally, ... Infertility after anti-sperm antibody binding can be caused by autoagglutination, sperm cytotoxicity, blockage of sperm-ovum ... They are protected by other means like immunologic tolerance and immunomodulation. ... Experiments are undergoing to test the effectiveness of an immunocontraceptive vaccine that inhibits the fusing of spermatozoa ...
When potentially suitable couple is found, crossmatch test is done to exclude that patient produces donor-specific anti-HLA ... 2010 Primer on Allergic and Immunologic Diseases. 125 (2, Supplement 2): S41-S52. doi:10.1016/j.jaci.2009.09.046. ISSN 0091- ... Complement-dependent cytotoxicity (CDC) is an effector function of IgG and IgM antibodies. When they are bound to surface ... CDC assay is usually used for performing crossmatch test. The basic version involves incubation of patient's serum with donor's ...
... cytotoxicity tests, immunologic MeSH E01.450.495.160.155 - complement hemolytic activity assay MeSH E01.450.495.225 - ... patch tests MeSH E01.450.495.750.800 - tuberculin test MeSH E01.450.620.620 - pregnancy tests, immunologic MeSH E01.450.865.100 ... placental function tests MeSH E01.370.378.620 - pregnancy tests MeSH E01.370.378.620.620 - pregnancy tests, immunologic MeSH ... immunologic MeSH E01.450.495.620 - pregnancy tests, immunologic MeSH E01.450.495.735 - serologic tests MeSH E01.450.495.735.050 ...
The degree of cytotoxicity is expressed as percentage PRA (panel reactive antibody). It is a tool that can be employed to ... Mechanisms of immunologic enhancement. Transplant Proc. 1970; 2(1):68-75. Kerman RH, Kimball PM, Van Buren CT, Lewis RM, DeVera ... Basic histocompatibility testing methods. In:Chandraker A, editor. Core concepts in renal transplantation. New York:Springer ... Significance of the positive crossmatch test in kidney transplantation. N Engl J Med. 1969; 280(14):735-9. Stiller CR, Sinclair ...
MAbs facilitate destruction of tumor cells by complement-dependent cytotoxicity (CDC) and cell-mediated cytotoxicity (ADCC). In ... IFNs have been tested as therapies for many types of cancers and have demonstrated therapeutic benefits. IL-2 has been approved ... Drugs used for immunotherapy can act either passively by enhancing the immunologic response to MET-expressing tumor cells, or ... Neutralizing anti-HGF antibodies were initially tested in combination, and it was shown that at least three antibodies, acting ...
The liver function tests are usually elevated. A low level of the protein albumin in the blood is common.[citation needed] The ... Impaired NK-cell cytotoxicity is the hallmark of HLH. All genetic defects for familial HLH are related to granule-dependent ... immunologic abnormalities, and lymphohistiocytosis. Most cases have been diagnosed between 4 months and 7 years of age, with a ... Molecular genetic testing for four of the causative genes, PRF1 (FHL2), UNC13D (FHL3), STX11 (FHL4), and STXBP2 (FHL5), is ...
Laboratory tests that might show abnormalities include blood count, kidney function, electrolyte, and liver enzyme tests. ... In trials these have been a type of T-cell capable of cytotoxicity. Inserting the DNA into the effector cell can be ... This effect has been attributed to making an immunologic space within which the cells populate. The process as a whole results ... Immunohistochemical testing may reveal TdT or CALLA antigens on the surface of leukemic cells. TdT is a protein expressed early ...
This process is known as antibody-dependent cell-mediated cytotoxicity (ADCC). FcγRIII on NK cells can also associate with ... Sun PD (2003). "Structure and function of natural-killer-cell receptors". Immunologic Research. 27 (2-3): 539-48. doi:10.1385/ ... the current paradigm that T cells do not express FcRs and these findings were never challenged and experimentally tested. ... Another process involving Fc receptors is called antibody-dependent cell-mediated cytotoxicity (ADCC). During ADCC, FcγRIII ...
Immunoglobulin light chain Immunoglobulin M Immunoglobulin Y Immunohaematology Immunoisolate Immunologic activation Immunologic ... CXCL16 CXCL17 CXCL2 CXCL3 CXCL5 CXCL6 CXCL7 CXCL9 CXCR4 CXCR6 CXCR7 Cytokine Cytokine redundancy Cytokine storm Cytotoxicity ... caseosa Viral phylodynamics Virus quantification Voltage-gated proton channel Wheat germ agglutinin White blood cell Widal test ... Anti-cholesterol Anti-gliadin antibodies Antibody Antibody opsonization Antibody-dependent cell-mediated cytotoxicity Antibody- ...
Allen, JG: Tests of Liver Function. Kansas City Academy, Kansas City. 57. Finkel, M. P., & Hirsch, G. M. (1950). URANIUM ... Reilly, C. A., Pritchard, D. J., Biskis, B. O., & Finkel, M. P. (1972). Immunologic evidence suggesting a viral etiology of ... USA). Pritchard, D. J., Reilly, C. A., Finkel, M. P., & Ivins, John C. (1974). Cytotoxicity of human osteosarcoma sera to ...
Animal toxicity studies: Acute toxicity testing, Repeat-dose toxicity testing, Long-term toxicity testing Pharmacokinetics and ... Rus H, Cudrici C, Niculescu F (2005). "The role of the complement system in innate immunity". Immunologic Research. 33 (2): 103 ... The "knobs-into-holes" shape facilitates antibody dependent cell mediated cytotoxicity. Single chain variable fragments (scFv) ... In vitro and in vivo testing for adventitious viruses, Murine retrovirus testing... Product safety data needed before the ...
The A4 study on older individuals who are positive for indicators of AD but are negative for genetic risk factors will test ... The four known IgG subclasses are involved in antibody-dependent cellular cytotoxicity.Antibodies are a key component of the ... murine molecules were engineered to remove immunogenic content and to increase immunologic efficiency. This was initially ... Major problems associated with murine antibodies included reduced stimulation of cytotoxicity and the formation complexes after ...
A cross-reaction test between potential donor cells and recipient serum seeks to detect presence of preformed anti-HLA ... the epitope can be recognized by immunologic structures like T-cell receptors (TCRs). The molecular region which binds to the ... causing damage via mechanisms such as direct cytotoxicity from CD8 cells. Acute humoral rejection and chronic disfunction ...
... to be administered to patients after testing for the BRAF genetic mutation. Tests of this sort are being widely advertised for ... and CR3-dependent cellular cytotoxicity. Complement-dependent cytotoxicity occurs when antibodies bind to the cancer cell ... T cells and immune system cytokines and have been investigated in clinical trials as immunologic adjuvants. Many tumors express ... In on-going trials the combination of CTLA-4 blockade with PD-1 or PD-L1 inhibitors is tested on different types of cancer. ...
This aided in the proper identification of transplant matches prior to the era of PCR-gene testing. Prior to refined typing for ... Czaja AJ, Carpenter HA, Santrach PJ, Moore SB (January 1995). "Immunologic features and HLA associations in chronic viral ... whereas class I mediated cytotoxicity is not. MICA and MICB are intestinally expressed. There are many genes that lie on either ... make it useful for making cell lines that can be used to test serotyping antibodies. As a result, HLA-A1 and B8 produce some of ...
Culture tests are rarely used as diagnostic tools; rather immunoblotting, immunofluorescent staining, hemadsorption tests, ... August 2004). "Impact of cethromycin (ABT-773) therapy on microbiological, histologic, immunologic, and respiratory indices in ... which would prevent initiation of cytotoxicity and subsequent symptoms. To date, vaccines targeted at the P1 adhesin have shown ... Causative diagnosis is dependent upon laboratory testing, however these methods are more practical in epidemiological studies ...
The CAMP test is also another important test for identification of GBS. The CAMP factor produced by GBS acts synergistically ... depending on the immunologic reactivity of their polysaccharide capsule.[1][4][5] This is why the plural term group B ... of Streptococcus urinary tract infection depends on bacterial strain and β-hemolysin/cytolysin that mediates cytotoxicity, ... Positive CAMP test indicated by the formation of an arrowhead where Streptococcus agalactiae meets the Staphylococcus aureus ( ...
Gaffen SL, Jain R, Garg AV, Cua DJ (September 2014). "The IL-23-IL-17 immune axis: from mechanisms to therapeutic testing". ... Strober W (November 2010). "The LTi cell, an immunologic chameleon". Immunity. 33 (5): 650-2. doi:10.1016/j.immuni.2010.11.016 ... their cytotoxicity, and their resident marker expression. NK cells are cytotoxic cells, circulating in the bloodstream, killing ...
Kinchen, Jason M.; Ravichandran, Kodi S. (October 2008). "Phagosome maturation: going through the acid test". Nature Reviews. ... They also suggested that the presence of neutralizing antibody or antibody-dependent cellular cytotoxicity-mediating antibodies ... March 2005). "Correlation between immunologic responses to a recombinant glycoprotein 120 vaccine and incidence of HIV-1 ... most relevant to in vitro tests but known to have some in vivo relevances Tirado SM, Yoon KJ (2003). "Antibody-dependent ...
Laboratory tests that might show abnormalities include blood count, kidney function, electrolyte, and liver enzyme tests.[17] ... This effect has been attributed to making an immunologic space within which the cells populate.[52] The process as a whole ... In trials these have been a type of T-cell capable of cytotoxicity.[53] ... Blood tests and bone marrow examination[3]. Differential diagnosis. Infectious mononucleosis, acute myeloid leukemia, ...
Even with standard blood compatibility testing, there is a risk of reaction against human blood group systems other than ABO ... 84(10):1215-9 - according to Sánchez-Fueyo A, Strom TB (2011), Immunologic basis of graft rejection and tolerance following ... Increased natural cytotoxicity receptor expression and relevant IL-10 production in NK cells from chronically infected viremic ... Immunologic basis of graft rejection and tolerance following transplantation of liver or other solid organs. Gastroenterology ...
However, these same events can lead to an inflammatory process in genetically predisposed individuals when the immunologic ... Avenins cytotoxicity in celiac people depends on the oat cultivar consumed because of prolamin genes, protein amino acid ... without having previously tested for celiac disease or doctor's recommendations.[54] People with NCGS and gastrointestinal ...
Sun PD (2003). "Structure and function of natural-killer-cell receptors". Immunologic Research. 27 (2-3): 539-48. doi:10.1385/ ... This process is known as antibody-dependent cell-mediated cytotoxicity (ADCC). FcγRIII on NK cells can also associate with ... the current paradigm that T cells do not express FcRs and these findings were never challenged and experimentally tested.[45] ... Induction of antibody-dependent cell-mediated cytotoxicity (ADCC). Induction of cytokine release by macrophages ...
Cytotoxicity Tests, Immunologic * Dose-Response Relationship, Drug * Electrophoresis, Polyacrylamide Gel * Enzyme-Linked ...
... cell tumor cytotoxicity in vitro was studied in elderly women after participation in a program of physical exercise training ( ... Cytotoxicity Tests, Immunologic * Cytotoxicity, Immunologic / immunology* * Exercise / physiology* * Exercise Test * Female * ... Physical exercise increases natural cellular-mediated tumor cytotoxicity in elderly women Gerontology. 1989;35(2-3):66-71. doi ... The influence of acute treadmill exercise on natural killer (NK) cell tumor cytotoxicity in vitro was studied in elderly women ...
Cytotoxicity Tests, Immunologic. The demonstration of the cytotoxic effect on a target cell of a lymphocyte, a mediator ... This protocol tests whether changes in BLyS or IFN can be detected in a normal immune response to a vaccine and, if so, whether ... This study will test the safety of the drug in people with IgA deficiency. ... Candidates will be screened with a physical examination, blood and urine tests, electrocardiogram (EKG), chest X-ray and a ...
Cytotoxicity Tests, Immunologic. Erythrocytes / immunology. Female. Hemolytic Plaque Technique. Immunoglobulin M. Mice. Mice, ... The effect of anti-C3 antibody was not due to cytotoxicity. These data collectively indicate that the effect of CoV on T- ...
Cytotoxicity Tests, Immunologic. Cytotoxicity, Immunologic*. Humans. Immunosuppressive Agents / therapeutic use. Isoantigens / ... Plasma from most SLE patients tested did not have an effect on CML and MLR responses of normal MNC; the plasma of only one ... Some of the patients exhibited parallel decreases in both CML and MLR tests, while others showed decreased CML responses but ...
Cytotoxicity Tests, Immunologic. *Epitopes/immunology. *Flow Cytometry. *Immunization. *Mice. *Mice, Inbred C57BL ...
Cytotoxicity Tests, Immunologic/methods. *Cytotoxicity, Immunologic/immunology. *Down-Regulation. *Glycosylation. * ...
This vaccine is currently being tested in animals and hopefully will be an effective contraceptive for humans. Normally, ... Infertility after anti-sperm antibody binding can be caused by autoagglutination, sperm cytotoxicity, blockage of sperm-ovum ... They are protected by other means like immunologic tolerance and immunomodulation. ... Experiments are undergoing to test the effectiveness of an immunocontraceptive vaccine that inhibits the fusing of spermatozoa ...
Immunologic techniques‎ (2 C, 146 F). *. ► Immunologic tests‎ (8 C, 8 F) ... el); gren af biomedicin (da) Immunologico (it); Immunologique, Immunobiologie (fr); Inmunologia (eu); Immunològica, ...
When potentially suitable couple is found, crossmatch test is done to exclude that patient produces donor-specific anti-HLA ... 2010 Primer on Allergic and Immunologic Diseases. 125 (2, Supplement 2): S41-S52. doi:10.1016/j.jaci.2009.09.046. ISSN 0091- ... Complement-dependent cytotoxicity (CDC) is an effector function of IgG and IgM antibodies. When they are bound to surface ... CDC assay is usually used for performing crossmatch test. The basic version involves incubation of patients serum with donors ...
... suggesting a higher sensitivity of cytotoxicity assays than that seen with the Elek test (15). Because DT production by ... but not by immunologic precipitation for this patient in vivo. Furthermore, as described first for C. diphtheriae, also ... ulcerans-negative using a modified Elek test, but positive in a cytotoxicity assay using Vero cells. Similar findings have been ... Antimicrobial susceptibility of the isolates was tested on Mueller-Hinton blood agar (supplemented with 5% sheep blood) after ...
Immunologic techniques‎ (2 t.l., 146 t.t.). *. ► Immunologic tests‎ (6 t.l., 5 t.t.) ...
Cytotoxicity Tests, Immunologic. Mielcarek M, Storb R, Georges GE, Golubev L, Nikitine A, Hwang B, Nash RA, Torok-Storb B. 2011 ... Cytotoxicity, Immunologic. Chattopadhyay PK, Betts MR, Price DA, Gostick E, Horton H, Roederer M, De Rosa SC. 2009. The ...
... is an immunologic cytotoxic effector mechanism that is dependent on the cooperative interaction of humoral and cellular ... This unit describes an assay of ADCC activity that can be used as a test for immunocompetence in effector cells or to test the ... Antibody‐dependent cell‐mediated cytotoxicity (ADCC) is an immunologic cytotoxic effector mechanism that is dependent on the ... Monocyte‐mediated antibody‐dependent cellular cytotoxicity: A clinical test of monocyte function. Blood 48:809‐816. ...
... cells and experiments for screening and validating novel biologically-based therapeutic targets by standard cytotoxicity tests ... The development of novel immunologic techniques and applying novel technologies will be included in this effort in addition to ...
Cytotoxicity Tests, Immunologic. Cytotoxicity, Immunologic / drug effects. Cytotoxicity, Immunologic / immunology. Epitopes / ... Immunologic Factor. A1.4.1.1.3.6. Receptor. A1.4.1.1.4. Indicator, Reagent, or Diagnostic Aid. A1.4.1.1.5. Hazardous or ... All data were analysed by paired Students t-test.. * A p-value of less than 0.05 was considered to indicate statistical ... A2.2.1. Laboratory or Test Result. A2.2.2. Sign or Symptom. A2.3. Organism Attribute. A2.3.1. Clinical Attribute. A2.4. ...
Cytotoxicity Tests, Immunologic. Female. Flow Cytometry. Fluorescein-5-isothiocyanate / metabolism. Fluorescent Dyes / ... Predictive Value of Tests. Prospective Studies. Reproducibility of Results. Signal Transduction / drug effects. Single-Cell ... Cellular ex vivo drug resistance was tested by means of the MTT assay. ...
A simple allogeneic test system for the study of antibody-dependent cellular cytotoxicity (ADCC) in man.. Láng, I., Fekete, B. ... Antibody dependent cellular cytotoxicity of lymphocytes in patients with renal diseases of immunopathological origin. Láng, I. ... Activity tests on concanavalin A-induced suppressor cells in autologous and allogenous systems. Gergely, P., Gonzalez Cabello, ... Action of cyclic nucleotides in antibody dependent cellular cytotoxicity reactions. Láng, I., Kalmar, L. & Gergely, P., 1978, ...
Delayed-type hypersensitivity reaction. To test the cell-mediated cytotoxicity response, 0.05 μg purified tuberculin and 10 μg ... The safety and immunologic responses of the study were discussed. Here, we describe the clinical evaluation of 24 malignant ... The log-rank test revealed that the survival curves for the two groups were significantly different (P = 0.010; Fig. 4). In the ... The log-rank test was used to assess the strength of the association between survival time and single variables corresponding ...
Immunologic Cytotoxicity Tests Transplantation Immunology Skin Transplantation Inbred Strains Mice Nucleoproteins ...
... where it mediates immunologic responses to pathogens. It interacts with IgA-opsonized targets and triggers several immunologic ... Other applications have not been tested. , Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage ... defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of ...
Selecting the nutrition for an animal or animal group comprises performing a diagnostic test to obtain first data. A data base ... IFA and FA testing; ELISA testing, cell cytotoxicity testing, cytokine testing (see Test 3, other cytotoxic cell and ... Test 3: Diagnostic Test Panels for Immunologic Function Patient phenotypic descriptors and genotypic descriptors/background, ... Test Panels The following are some specific diagnostic test panels and specialized diagnostic tests and test groups used to ...
IFA and FA testing; ELISA testing, cell cytotoxicity testing, cytokine testing (see Test 3, other cytotoxic cell and ... The tests above, which relate to at least one of endocrine function, immunologic function, gastrointestinal function and ... Clostridium perfringens enterotoxin test; cryptosporidiosis test (FA); toxoplasmosis test; bile acids test (resting and post- ... dilute whole blood lysis test, euglobulin lysis test); anti-thrombin III test; circulating anticoagulant tests; platelet ...
Statistics. We used the Mann-Whitney test to compare quantitative variables across groups, Kruskal-Wallis test for quantitative ... One limitation of our study is that our overall picture of immunologic memory is that of the blood; it does not reflect tissue- ... Of note, mouse CD4+ effectors confer protection from lethal influenza through perforin-mediated cytotoxicity (30). ... Statistical analyses used the Mann-Whitney U test and statistical significance is indicated. **P , 0.01. (B and D) Radar charts ...
Cytotoxicity Assay and Class I MHC Blocking.. Cytotoxicity was measured in a standard 4-h 51Cr release assay. In brief, T2 ... we used nonparametric tests based on u-scores, i.e., Spearman correlation and Mann-Whitney test, as indicated. To score ... Immunologic and therapeutic evaluation of a synthetic peptide vaccine for the treatment of patients with metastatic melanoma. ... c) Killing of melanoma peptide-pulsed T2 cells (a mix of four melanoma peptides) by cultured CD8+ T cells from all tested ...
Why, then, has so much attention been given to the study of immunologic changes in elderly humans and... ... Immunologic function declines with age. Indeed, most physiologic functions decline with age. ... Stead WW, To T. The significance of the tuberculin skin test in elderly persons. Ann Intern Med 1987; 107: 837-42.PubMedGoogle ... A tube of venous blood provides the immunologist with millions of cells with which to study antibody production, cytotoxicity, ...
Keywords : glass ionomer cements; cytotoxicity tests; immunologic; cell culture techniques. · abstract in Portuguese · text in ... Conclusion: All glass ionomer cements tested were toxic prior to the setting reaction.However after setting reaction, allowed ...
... cytotoxicity, immunologic; enzyme-linked immunosorbent assay; antigen binding; humoral immunity; antibody formation; hla-a2 ... antigen; immunity, cellular; histocompatibility testing; humans; priority journal; article Journal Title:. Proceedings of the ... cd8 antigen; cd8-positive t-lymphocytes; reproducibility; proteins; cells, cultured; melanoma; membrane proteins; cytotoxicity ... and reproducibility have been developed and found to correlate with cytotoxicity assays. CD8+ T cell responses to HLA-A2- ...
Cytotoxicity Tests, Immunologic 3. Estudio comparaiivo de los métodos de resazurin y mtt en estudios de citotoxiccidad en ... Assessing two staining methods for cytotoxicity tests on tumor cell lines Escobar, M. Linamaría; Alfonso R., Paola A; ... A correlation test using the data obtained from the assays showed that there was no significant difference between the methods ... Performing cloning efficiency tests, plating efficiency and growth curves of cell lines and fibroblasts, showed that the ...
Antibody-Dependent Cell Cytotoxicity/genetics*. *Antigens, CD20/immunology. *Cytotoxicity Tests, Immunologic*. *Feasibility ... Thus, the use of an ADCC assay as a lot release test or a stability test for clinical trial batches of mAbs has been a ... We tested the biological relevance of this assay using an anti-CD20 based model and demonstrated that this ADCC-reporter assay ... Development and validation of an antibody-dependent cell-mediated cytotoxicity-reporter gene assay.. Parekh BS1, Berger E, ...
  • Cross-match testing with cytotoxic analysis has long been supplemented by flow cytometry, but development of solid-phase single-bead antigen testing of solubilized human leukocyte antigens (HLA) to detect donor-specific antibodies (DSA) permits a far more nuanced stratification of immunological risk status, including the different classes and intensities of HLA antibodies Class I and/or II, including HLA-DSA. (uni-frankfurt.de)
  • When this was removed by absorption, the sera showed activity against three of the four HLA-A and -B antigens in the immunogen when tested on a panel of peripheral blood lymphocytes and T cells. (ox.ac.uk)
  • Antibodies recognizing HLA-DR antigens were detected by testing platelet absorbed sera on a panel of typed lymphoblastoid cell lines. (ox.ac.uk)
  • This work involves skills that include sterile tissue culture, preparation of cells and experiments for screening and validating novel biologically-based therapeutic targets by standard cytotoxicity tests, flow cytometry, cytof, and immunoblottings. (postdocjobs.com)
  • This vaccine is currently being tested in animals and hopefully will be an effective contraceptive for humans. (wikipedia.org)
  • Why, then, has so much attention been given to the study of immunologic changes in elderly humans and laboratory animals? (springer.com)
  • These uses have been tested in humans or animals. (naturesmarketholland.com)
  • They often have not been thoroughly tested in humans, and safety and effectiveness have not always been proven. (naturesmarketholland.com)
  • Overall, there is a shift towards planning the immunosuppressive regimen based on pre-transplant immunology testing although certain conventional risk factors retain their importance. (uni-frankfurt.de)
  • This requires an understanding of basic immunology, the mechanism of action and side effects of immunosuppressive medications, and a thorough immunologic risk assessment. (oncologynurseadvisor.com)
  • The methyl thiazolyl tetrazolium (MTT) assay was used to assess the cytotoxicity of these materials for human gingival fibroblasts (HGFs). (fluoridealert.org)
  • CDC assay is usually used for performing crossmatch test. (wikipedia.org)
  • This unit describes an assay of ADCC activity that can be used as a test for immunocompetence in effector cells or to test the activity of a monoclonal antibody to mediate ADCC. (currentprotocols.com)
  • This prospective cooperative study was initiated in 1978 to determine the value of serologic tests related to Epstein-Barr virus (EBV), including the antibody response to the EBV membrane antigen as measured by the antibody-dependent cellular cytotoxicity (ADCC) assay, in North American patients with different histopathologic types of nasopharyngeal carcinoma (NPC). (springer.com)
  • Development and validation of an antibody-dependent cell-mediated cytotoxicity-reporter gene assay. (nih.gov)
  • Thus, the use of an ADCC assay as a lot release test or a stability test for clinical trial batches of mAbs has been a substantial challenge to install in quality control laboratories. (nih.gov)
  • This data demonstrates that the ADCC-reporter gene assay has performance characteristics (accuracy, precision and robustness) to be used not only as a potency assay for lot release and stability testing for antibody therapeutics, but also as a key assay for the characterization and process development of therapeutic molecules. (nih.gov)
  • However, performance of an enzyme immunoassay is the usual method by which CDI is confirmed, but this test appears to be relatively insensitive, compared with the cell cytotoxicity assay and stool culture for toxigenic C. difficile on selective medium. (nih.gov)
  • Immunologic risk evaluation is now often based on a combination of these tests, but other assessments are becoming more widely introduced, such as measurement of non-HLA antibodies against angiotensin type 1 (AT1) receptors or T-cell ELISPOT assay of alloantigen-specific donor. (uni-frankfurt.de)
  • Sera and colonic tissue-bound immunoglobulin extracts from patients with ulcerative colitis and disease controls were examined immunohistochemically and by killer cell cytotoxicity assay for the presence of anticolonic epithelial autoantibodies. (ox.ac.uk)
  • 400 different antibody combinations were tested in a standard viability assay in vitro . (aacrjournals.org)
  • Complement-dependent cytotoxicity (CDC) is an effector function of IgG and IgM antibodies. (wikipedia.org)
  • When potentially suitable couple is found, crossmatch test is done to exclude that patient produces donor-specific anti-HLA antibodies, which could cause graft rejection. (wikipedia.org)
  • There are modifications available to increase test sensitivity including extension of minimal incubation time, adding antihuman globulin (AHG), removing unbound antibodies before adding complement, separation of T cell and B cell subset. (wikipedia.org)
  • In this form of cytotoxicity, effector cells with receptors for the Fc portion of immunoglobulin produce target cell lysis by attachment to the Fc portion of antibodies that are bound to target cells via their antigen‐combining sites. (currentprotocols.com)
  • Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important biological function attributed to the mechanism of action of several therapeutic antibodies, particularly oncology targeting mAbs. (nih.gov)
  • To further assess the state of viral activity in latent infection we have tested the activity of monoclonal antibodies against putative latent viral proteins. (duke.edu)
  • 22 Additionally, titers of antibodies that mediate antibody-dependent cellular cytotoxicity inversely correlate with severity of neonatal infection. (menopausealliance.org)
  • An immunologic response in which an immunologic effector cell binds to a target cell coated with antibodies, triggering a series of metabolic events that leads to lysis of the target cell. (thefreedictionary.com)
  • Using Xencor's XmAb Fc enhancement technology, MOR208 has been engineered to possess significantly enhanced antibody-dependent cell-mediated cytotoxicity (ADCC), thus improving a key mechanism for tumour cell killing and offering potential for enhanced efficacy compared to traditional antibodies for the treatment of cancer. (thefreedictionary.com)
  • The influence of acute treadmill exercise on natural killer (NK) cell tumor cytotoxicity in vitro was studied in elderly women after participation in a program of physical exercise training (PET) (n = 7) or after participation in a paralleling nonexercise control (NEC) condition (n = 7). (nih.gov)
  • We conclude that natural cellular-mediated tumor cytotoxicity is increased in response to acute exercise and long-term PET in elderly women. (nih.gov)
  • Among the new treatments currently being investigated for malignant glioma, immunotherapy is theoretically very attractive because it offers the potential for high tumor-specific cytotoxicity ( 3 - 7 ). (aacrjournals.org)
  • NCI conducted tests of the constituents for antitumor activity in a mouse tumor model (the Sarcoma 37 test), and found that all were highly active in that test system (384). (quackwatch.org)
  • mAb-mediated engagement of immunologic effector cells by FcγR binding resulting in antibody-dependent cellular cytotoxicity toward the tumor is another proposed mechanism of cetuximab ( 8 ). (aacrjournals.org)
  • Three lead candidate mixtures with high efficacy in vitro were selected and tested for their ability to inhibit tumor growth in vivo . (aacrjournals.org)
  • Avelumab has also been shown to induce NK cell-mediated direct tumor cell lysis via antibody-dependent cell-mediated cytotoxicity in vitro. (thefreedictionary.com)
  • The effect of anti-C3 antibody was not due to cytotoxicity. (biomedsearch.com)
  • Infertility after anti-sperm antibody binding can be caused by autoagglutination, sperm cytotoxicity, blockage of sperm-ovum interaction, and inadequate motility. (wikipedia.org)
  • Contrast with antibody-dependent cellular cytotoxicity (ADCC) Schroeder, Harry W. (wikipedia.org)
  • Zhou 2008 Complement dependent cytotoxicity activity of therapeutic antibody fragments is acquired by immunogenic glycan coupling. (wikipedia.org)
  • Antibody‐dependent cell‐mediated cytotoxicity (ADCC) is an immunologic cytotoxic effector mechanism that is dependent on the cooperative interaction of humoral and cellular effector elements. (currentprotocols.com)
  • A tube of venous blood provides the immunologist with millions of cells with which to study antibody production, cytotoxicity, proliferation, migration, and other characteristics that are necessary for the continued health and survival of an organism. (springer.com)
  • It interacts with IgA-opsonized targets and triggers several immunologic defense processes, including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and stimulation of the release of inflammatory mediators. (biomol.com)
  • Polymorphonuclear leukocytes in antibody-dependent cellular cytotoxicity. (naver.com)
  • Serial immunologic testing included assessment of natural killer (NK) cell activity, antibody-dependent cytotoxicity, delayed hypersensitivity, and in vitro mitogen responses. (faintpower.gq)
  • By retaining a native Fc-region, avelumab is thought to potentially engage the innate immune system and induce antibody-dependent cell-mediated cytotoxicity (ADCC). (thefreedictionary.com)
  • Berne, " Antibody-dependent cell-mediated cytotoxicity tests for immunosuppression monitoring of transplant patients," Transplantation, vol. (thefreedictionary.com)
  • Wick, "Endothelial cell apoptosis in systemic sclerosis is induced by antibody-dependent cell-mediated cytotoxicity via CD95," Arthritis & Rheumatism, vol. (thefreedictionary.com)
  • Recognition of known epitopes within longer synthesized peptides by peripheral blood mononuclear cells or CD8 T cell lines was tested using interferon-gamma Elispot at various peptide concentrations. (ox.ac.uk)
  • Plasma and, to a lesser extent, peripheral lymphocytes from 2 affected members who were tested were cytotoxic to allogeneic colonic epithelial cells. (unboundmedicine.com)
  • Their Fab fragments blocked the cytotoxicity of the allogeneic antisera for B lymphocytes and were potent inhibitors of the mixed lymphocyte reaction. (ox.ac.uk)
  • Choosing the proper immunosuppression for a patient involves balancing the risk of immunologic graft injury against the risks of infection and malignancy. (oncologynurseadvisor.com)
  • The receptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils, monocytes, macrophages, and eosinophils, where it mediates immunologic responses to pathogens. (biomol.com)
  • Performing cloning efficiency tests, plating efficiency and growth curves of cell lines and fibroblasts, showed that the Colombian serum can be used with a good performance, in continuous culture of cell lines A549, PC-3, HT-29, MDA-MB231, in fibroblasts LSPG8G cells, MDBK and hybridomes can be used in a minimum concentration of 10% and medium every 48 hours. (bvsalud.org)
  • Next, we compared survival results by using reduction method MTT and staining method SRB defined as end-point tests, and resazurin method described as slightly toxic to cells. (bvsalud.org)
  • Specifically, we show that human natural killer cells have the ability to produce IGF-1 and that differential endogenous IGF-1 expression leads to disparate cytotoxicity in human primary natural killer cells. (isharonline.org)
  • The cytotoxic activity of Immu-21 was tested by measuring the splenic leukocyte natural killer (NK) cell activity against K 562 cells. (isharonline.org)
  • The aim of this study was to test whether the negative selection of T cells will produce T cells that are resistant to inhibition by extracellular adenosine. (isharonline.org)
  • CD66a interactions between human melanoma and NK cells: a novel class I MHC-independent inhibitory mechanism of cytotoxicity. (ox.ac.uk)
  • 49 , 45 , 50 , 51 , 52 , 53 Purified lectins- ML-I (D-galactose specific), ML-II and III (N-acetyl-D-galactosamine-specific) proteins demonstrated cytotoxicity in killing cells, which was mediated by induction of apoptosis as measured by appearance of hypodiploid nuclei (DNA peak) in flow cytometery. (sigmaaldrich.com)
  • By contrast, adaptive immune cells display immunologic memory that has 2 basic characteristics, antigen specificity and an amplified response upon subsequent exposure. (pnas.org)
  • CD8 T cells bind to class I MHC molecules and generally mediate cytotoxicity. (oncologynurseadvisor.com)
  • Targeted densensitization protocols can improve immunological risk, notably for DSA-positive patients with negative cytotoxicity and flow cross-match. (uni-frankfurt.de)
  • Immunological risk is estimated from the patient's medical history, immunogenetic testing, characteristics of the donor organ (living versus deceased donor), and time elapsed since transplant. (oncologynurseadvisor.com)
  • Assessment of in vitro cytotoxicity as a model to explore biological activity of compounds isolated from different sources is the first approached to search molecules with potential anticancer activity. (bvsalud.org)
  • Achieving Biocompatible SABRE: An in vitro Cytotoxicity Study. (harvard.edu)
  • Specific immunologic factors responsible for immunity to herpes simplex virus are not completely understood. (menopausealliance.org)
  • Std test nearby Mansfield, LA . Herpes simplex virus is also believed to suppress innate immunity by suppressing the production of interferon-alfa and interferon-beta. (menopausealliance.org)
  • To investigate the safety and the immunologic and clinical responses of dendritic cell therapy for patients with recurrent malignant glioma. (aacrjournals.org)
  • A large body of clinical evidence, histopathologic data, and more recently immunologic studies supports the concept that carcinomas of the nasopharynx constitute two distinct diseases. (springer.com)
  • These include tests on clinical drugs, foods, and environmental pollutants. (harvard.edu)
  • With dedicated space in the clinical laboratories and Center for Advanced Molecular Diagnostics (CAMD) at Brigham & Women's Hospital, the unit offers CLIA-level (Clinical Laboratory) handling for clinical trials and for diagnostic testing. (partners.org)
  • Assays offered in the BWH Clinical Laboratories are also available through the CLIA Testing Unit . (partners.org)
  • Testing includes standard hematology, clinical chemistry and immunologic testing. (partners.org)
  • At the time of KT and at pre-specified intervals, clinical information, laboratory test results, and functional and imaging studies on cardiovascular disease and metabolic complications will be recorded. (biomedcentral.com)
  • 1. Mastery of all theoretic aspects of the allergic and clinical immunologic evaluation including prevention, diagnosis and treatment, and systemic manifestations of allergic and clinical immunologic disease. (dypatil.edu)
  • The purpose of this document is to suggest to the device manufacturer or investigation sponsor important preclinical and clinical tests that should be performed to generate data that will provide reasonable assurance of the safety and effectiveness of these devices for their intended purposes. (fda.gov)
  • In May of 1986, DSRD completed a first draft of the document containing preclinical and clinical testing recommendations and suggestions for the preparation of IDEs and PMAs. (fda.gov)
  • Serum samples from about 10 males and 10 females from each of 16 genetically defined strains of rabbits and from one hybrid were tested as sources of complement for the microtiter lymphocytotoxicity test. (jax.org)
  • NY-ESO-1 ELISPOT and tetramer assays with excellent sensitivity, specificity, and reproducibility have been developed and found to correlate with cytotoxicity assays. (mskcc.org)
  • Some of the patients exhibited parallel decreases in both CML and MLR tests, while others showed decreased CML responses but normal MLR responses. (biomedsearch.com)
  • The safety and immunologic responses of the study were discussed. (aacrjournals.org)
  • Cytotoxicity may also occur as a result of lectins damaging cell membranes with an influx of Ca 2+ and cell shrinkage. (sigmaaldrich.com)
  • 2010 Primer on Allergic and Immunologic Diseases. (wikipedia.org)
  • These findings suggest the existence of a novel class I MHC-independent inhibitory mechanism of human NK cell cytotoxicity. (ox.ac.uk)
  • The cytotoxicity of azathioprine is due, in part, to the incorporation of 6-TGN into DNA. (nih.gov)
  • The effect of azathioprine on these variables has not been tested in controlled trials. (rxlist.com)
  • An in vitro test used in the diagnosis of allergies including drug hypersensitivity. (umassmed.edu)
  • Cytotoxicity of sodium fluoride on human oral mucosal fibroblasts and its mechanisms. (fluoridealert.org)
  • Experiments are undergoing to test the effectiveness of an immunocontraceptive vaccine that inhibits the fusing of spermatozoa to the zona pellucida. (wikipedia.org)
  • Chemical constituents with antitumor activity were isolated from the plant and tested for antitumor effects in animal systems. (quackwatch.org)
  • Higher fungi, including both edible and inedible mushrooms, are some of the major sources of polysaccharides and other substances that have been studied for antitumor and immunologic activity and as potential sources of new anticancer drugs. (quackwatch.org)
  • Effect of rabbit strain on activity level and cytotoxicity of serum c" by R R. Fox, M Cherry et al. (jax.org)
  • Effect of rabbit strain on activity level and cytotoxicity of serum complement. (jax.org)
  • We discovered that 45 FDA-approved drugs (4% of all drugs tested) in our FDA-approved libraries enhanced DNMTi and HDACi activity, mainly belonging to anticancer and antiarrhythmic drug classes. (aacrjournals.org)
  • Testing of stool for the presence of C. difficile toxin confirms the diagnosis of CDI. (nih.gov)