Cytokinins
Zeatin
Isopentenyladenosine
Plant Growth Regulators
Plant Roots
Plant Leaves
Gene Expression Regulation, Plant
Helleborus
Arabidopsis
Indoleacetic Acids
Plants, Genetically Modified
Arabidopsis Proteins
Meristem
Xylem
Alkyl and Aryl Transferases
Crassulaceae
Plant Proteins
Tobacco
Abscisic Acid
Coleus
Plant Stems
Naphthaleneacetic Acids
Photoreceptors, Plant
Plant Development
Bryopsida
A class of plants within the Bryophyta comprising the mosses, which are found in both damp (including freshwater) and drier situations. Mosses possess erect or prostrate leafless stems, which give rise to leafless stalks bearing capsules. Spores formed in the capsules are released and grow to produce new plants. (Concise Dictionary of Biology, 1990). Many small plants bearing the name moss are in fact not mosses. The "moss" found on the north side of trees is actually a green alga (CHLOROPHYTA). Irish moss is really a red alga (RHODOPHYTA). Beard lichen (beard moss), Iceland moss, oak moss, and reindeer moss are actually LICHENS. Spanish moss is a common name for both LICHENS and an air plant (TILLANDSIA usneoides) of the pineapple family. Club moss is an evergreen herb of the family LYCOPODIACEAE.
Zea mays
Oxidoreductases
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Seeds
Plant Tumors
RNA, Plant
Plant Immunity
Hypocotyl
The region of the stem beneath the stalks of the seed leaves (cotyledons) and directly above the young root of the embryo plant. It grows rapidly in seedlings showing epigeal germination and lifts the cotyledons above the soil surface. In this region (the transition zone) the arrangement of vascular bundles in the root changes to that of the stem. (From Concise Dictionary of Biology, 1990)
beta-Fructofuranosidase
Plants
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Ethylenes
Gibberellins
Chlorophyll
PubMed
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Cytokinin activation of Arabidopsis cell division through a D-type cyclin. (1/583)
Cytokinins are plant hormones that regulate plant cell division. The D-type cyclin CycD3 was found to be elevated in a mutant of Arabidopsis with a high level of cytokinin and to be rapidly induced by cytokinin application in both cell cultures and whole plants. Constitutive expression of CycD3 in transgenic plants allowed induction and maintenance of cell division in the absence of exogenous cytokinin. Results suggest that cytokinin activates Arabidopsis cell division through induction of CycD3 at the G1-S cell cycle phase transition. (+info)Detection of membrane-bound cytokinin-binding proteins in Arabidopsis thaliana cells. (2/583)
In order to isolate cytokinin-binding proteins (CBPs), we have developed new affinity probes constituted of a cytokinin such as zeatin riboside ([9R]Z) conjugated to a carrier protein. These probes were used for detecting CBPs in an ELISA procedure. The efficiency of the cytokinin conjugate in detecting CBPs was controlled with protein model: proteins having an affinity for cytokinin such as the monoclonal anti-[9R]Z antibodies did bind the cytokinin conjugate whereas proteins unable to bind cytokinin such as bovine serum albumin did not. Using these new affinity probes, we showed that CBPs are present in the membrane fraction of in vitro cultured Arabidopsis thaliana cells. The nature of the protein at the detected binding sites was demonstrated by submitting the microsomal proteins to a proteolytic treatment, which was found to eradicate the binding. Free biologically active cytokinins or monoclonal anti-[9R]Z antibodies inhibited the binding, thus showing the specificity of the interaction. The detected CBPs were partially solubilized from the membranes with potassium chloride, indicating their peripheral membrane location. The separation by anion exchange chromatography of solubilized microsomal proteins revealed the existence of two different CBPs. They were present at higher levels in cells during the exponential growth phase. (+info)A gene encoding the cytokinin enzyme zeatin O-xylosyltransferase of Phaseolus vulgaris. (3/583)
Zeatin is the most active and ubiquitous form of the naturally occurring cytokinins. Glycosyl conjugates of zeatin are found in many plant tissues and are considered important for storage and protection against degradative enzymes. Two enzymes catalyzing the formation of O-glycosyl derivatives of zeatin have been characterized, O-glucosyltransferase and O-xylosyltransferase, occurring in seeds of lima bean (Phaseolus lunatus) and bean (Phaseolus vulgaris), respectively. Recently, the ZOG1 gene (zeatin O-glucosyltansferase) was isolated from P. lunatis (). Based on the ZOG1 sequence, the ZOX1 gene (zeatin O-xylosyltransferase) was cloned from P. vulgaris. ZOX1 contains an open reading frame of 1362 bp that codes for a 454-amino acid peptide of 51 kD. The recombinant protein has properties identical to the native enzyme: it catalyzes O-xylosylzeatin formation with UDP-Xyl as a glycosyl donor but does not recognize UDP-Glucose as a substrate. The ZOX1 and ZOG1 genes exhibit 93% identity at the nucleotide level and 90% similarity at the amino acid level. Neither gene contains introns. These zeatin-specific genes and their promoters will be useful for studies of the regulation of active versus storage forms of cytokinins. Comparison of sequences encoding similar enzymes with distinct substrate specificity may lead to identification of epitopes specific to cytokinin and glycosyl donor molecules. (+info)Leaf senescence is delayed in tobacco plants expressing the maize homeobox gene knotted1 under the control of a senescence-activated promoter. (4/583)
Leaf senescence is an active process involving remobilization of nutrients from senescing leaves to other parts of the plant. Whereas senescence is accompanied by a decline in leaf cytokinin content, supplemental cytokinin delays senescence. Plants that overexpress isopentenyl transferase (ipt), a cytokinin-producing gene, or knotted1 (kn1), a homeobox gene, have many phenotypes in common. Many of these phenotypes are characteristic of altered cytokinin physiology. The effect of kn1 on leaf senescence was tested by driving its expression using the promoter of the senescence-associated gene SAG12. SAG:kn1 tobacco plants showed a marked delay in leaf senescence but otherwise developed normally. The delay in senescence was revealed by an increase in chlorophyll content in SAG:kn1 leaves relative to leaves of the control plants and by a decrease in the number of dead leaves. Senescence was also delayed in detached leaves of SAG:kn1 plants. Delayed senescence was accompanied by increased leaf cytokinin content in older leaves expressing kn1. These experiments extend the current understanding of kn1 function and suggest that in addition to mediating meristem maintenance, kn1 is capable of regulating the onset of senescence in leaves. (+info)Multiubiquitin chain binding subunit MCB1 (RPN10) of the 26S proteasome is essential for developmental progression in Physcomitrella patens. (5/583)
The 26S proteasome, a multisubunit complex, is the primary protease of the ubiquitin-mediated proteolytic system in eukaryotes. We have recently characterized MCB1 (RPN10), a subunit of the 26S complex that has affinity for multiubiquitin chains in vitro and as a result may function as a receptor for ubiquitinated substrates. To define the role of MCB1 further, we analyzed its function in Physcomitrella patens by generating MCB1 gene disruptions using homologous recombination. PpMCB1, which is 50 to 75% similar to orthologs from other eukaryotes, is present in the 26S proteasome complex and has a similar affinity for multiubiquitin chains, using a conserved hydrophobic domain within the C-terminal half of the polypeptide. Unlike yeast Deltamcb1 strains, which grow normally, P. patens Deltamcb1 strains are viable but are under developmental arrest, generating abnormal caulonema that are unable to form buds and gametophores. Treatment with auxin and cytokinin restored bud formation and subsequent partial development of gametophores. Complementation of a Deltamcb1 strain with mutated versions of PpMCB1 revealed that the multiubiquitin chain binding site is not essential for the wild-type phenotype. These results show that MCB1 has an important function in the 26S proteasome of higher order eukaryotes in addition to its ability to bind multiubiquitin chains, and they provide further support for a role of the ubiquitin/26S proteasome proteolytic pathway in plant developmental processes triggered by hormones. (+info)Cytokinins in tobacco and wheat chloroplasts. Occurrence and changes due to light/dark treatment. (6/583)
Although cytokinins (CKs) affect a number of processes connected with chloroplasts, it has never been rigorously proven that chloroplasts contain CKs. We isolated intact chloroplasts from tobacco (Nicotiana tabacum L. cv SR1) and wheat (Triticum aestivum L. cv Ritmo) leaves and determined their CKs by liquid chromatography/tandem mass spectroscopy. Chloroplasts from both species contained a whole spectrum of CKs, including free bases (zeatin and isopentenyladenine), ribosides (zeatin riboside, and isopentenyladenosine), ribotides (isopentenyladenosine-5'-monophosphate, zeatin riboside-5'-monophosphate, and dihydrozeatin riboside-5'-monophosphate), and N-glucosides (zeatin-N(9)-glucoside, dihydrozeatin-N(9)-glucoside, zeatin-N(7)-glucoside, and isopentenyladenine-N-glucosides). In chloroplasts there was a moderately higher relative amount of bases, ribosides, and ribotides than in leaves, and a significantly increased level of N(9)-glucosides of zeatin and dihydrozeatin. Tobacco and wheat chloroplasts were prepared from leaves at the end of either a dark or light period. After a dark period, chloroplasts accumulated more CKs than after a light period. The differences were moderate for free bases and ribosides, but highly significant for glucosides. Tobacco chloroplasts from dark-treated leaves contained zeatin riboside-O-glucoside and dihydrozeatin riboside-O-glucoside, as well as a relatively high CK oxidase activity. These data show that chloroplasts contain a whole spectrum of CKs and the enzymatic activity necessary for their metabolism. (+info)Auxin and cytokinin have opposite effects on amyloplast development and the expression of starch synthesis genes in cultured bright yellow-2 tobacco cells. (7/583)
In cultured Bright Yellow-2 (BY-2) tobacco (Nicotiana tabacum) cells, the depletion of auxin (2,4-dichlorophenoxyacetic acid) in the culture medium induces the accumulation of starch. This is accelerated by the addition of cytokinin (benzyladenine). Light and electron microscopic observations revealed that this amyloplast formation involves drastic changes in plastid morphology. The effects of auxin and cytokinin on amyloplast development were investigated by adding auxin or cytokinin to cells grown in a hormone-free culture. Auxin repressed amyloplast development, whereas cytokinin accelerated starch accumulation regardless of the timing of hormone addition. RNA gel-blot analysis revealed that the accumulation of the ADP-glucose pyrophosphorylase small subunit gene (AgpS), granule-bound starch synthase, and starch branching enzyme transcripts were also affected by hormonal conditions. High levels of AgpS, granule-bound starch synthase, and starch branching enzyme transcripts accumulated in amyloplast-developing cells grown in auxin-depleted conditions. Furthermore, the addition of auxin to the cells cultured in hormone-free medium reduced the level of AgpS transcripts, whereas the addition of cytokinin increased it, irrespective of the timing of hormone addition. These results suggest that auxin and cytokinin exert opposite effects on amyloplast development by regulating the expression of the genes required for starch biosynthesis. (+info)Cell-division factors from Vinca rosca L. crown gall tumor tissue. (8/583)
A cell-division factor has been precipitated from extracts of cultured Vinca rosea L. crown gall tumor tissue by using the mercuric acetate procedure previously employed by Wood and colleagues to obtain their "cytokinesin I." On the basis of its mass spectrum, ultraviolet light absorbancy spectra, solubilities, chromatographic migration values, and growth activity, the factor is ribosyl-trans-zeatin, that is, 6-(4-hydroxy-3-methyl-trans-2-butenylamino)-9-beta-D-ribofuranosylpurine. Ribosylzeatin has now been isolated from tumor tissue by four experimental techniques; any possibility that it is an artifact seems to have been eliminated. Contrary to the report by Wood and colleagues, synthetic ribosylzeatin is precipitated from an aqueous solution by mercuric acetate, provided the complete precipitation procedure is utilized. These facts and others discussed strongly support our suggestion that ribosylzeatin was present in the preparation ("cytokinesin I") examined by Wood and colleagues in several biological assays. The reasons advanced by Wood and others for rejecting this suggestion have been found either not to be pertinent to the question or to have insufficient experimental bases. (+info)
CKI1, a Histidine Kinase Homolog Implicated in Cytokinin Signal Transduction | Science
The Arabidopsis Histidine Phosphotransfer Proteins Are Redundant Positive Regulators of Cytokinin Signaling | Plant Cell
Cytokinin Regulates Type-A Arabidopsis Response Regulator Activity and Protein Stability via Two-Component Phosphorelay | Plant...
6-benzylaminopurine and its glycosides as naturally occurring cytokinins<...
Ectopic expression of KNOTTED1-like homeobox protein induces expression of cytokinin biosynthesis genes in rice<...
Endogenous auxin and cytokinin contents associated with shoot formation in leaves of pineapple cultured in vitro
Cytokinin and the transcriptional regulation of chloroplast development in Arabidopsis. :: Dartmouth Dissertations
Identification of Cytokinin-Responsive Genes Using Microarray Meta-Analysis and RNA-Seq in Arabidopsis | Plant Physiology
Investigation of cytokinin receptor from maize
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Trade-offs between plant defense and growth: hormonal regulation and molecular switches - Department of Biology | CSU
Use of a probabilistic motif search to identify histidine phosphotransfer domain-containing proteins
Cytokinin oxidase/dehydrogenase genes in barley and wheat: cloning and heterologous expression. | Sigma-Aldrich
CPIB » Plant Development: How Long Is a Root?
CPIB » Cytokinin signalling inhibitory fields provide robustness to phyllotaxis
Influence of cytokinins on the expression of phosphate starvation responsive genes in Arabidopsis. - Semantic Scholar
Cytokinins Secreted by Agrobacterium Promote Transformation by Repress by Nagesh Sardesai, Lanying Lee et al.
Professor Yrjö Helariuttas team recruited to Cambridge - GARNet
Metabolism and Molecular Activities of Cytokinins: Proceedings of the International Colloquium of the Centre National de La...
CiNii 論文 -
Overexpression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin...
Use of a Probabilistic Motif Search to Identify Histidine Phosphotransfer Domain-Containing Proteins
Whole Grains: More Productive Rice, Oat Genome Mapping - North Carolina Research Campus
Arabidopsis Cytokinin Signaling Pathway | Science Signaling
Studies of the role of tRNA-isopentenyl-transferases in the formation of cytokinins - Epsilon Archive for Student Projects
Cytokinins
Frontiers | Improved Shoot Regeneration, Salinity Tolerance and Reduced Fungal Susceptibility in Transgenic Tobacco...
Characterization of the cytokinin-responsive transcriptome in rice
Farnesylation directs AtIPT3 subcellular localization and modulates cytokinin biosynthesis in Arabidopsis - Research Collection
Patent US20090317863 - Use of active cytokinin synthase gene - Google Patents
cytokinin antibodies
Plant Physiology
Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize :...
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Molecular characterization of His-Asp phosphorelay signaling factors in maize leaves: implications of the signal divergence by...
Structure Cluster
- 1I5N: Crystal structure of the P1 domain of CheA from Salmonella typhimurium 3D Similarity Report...
RCSB PDB
- 1I5N: Crystal structure of the P1 domain of CheA from Salmonella typhimurium Macromolecule Annotations Page
E-MTAB-4298 - Transcription profiling by high throughput sequencing of Arabidopsis shoots and roots in response to cytokinin -...
IMPRS 2003 | Max Planck Institute for Plant Breeding Research
Light-Regulated Alternative Splicing of Pseudo-Histidine Phosphotransfer Protein 3 in Oryza sativa | SpringerLink
Perception and signal transduction of cytokinins. - PubMed - NCBI
Network Portal - Function cytokinin metabolic process
Biological Foundations 112, Lecture 18
Solved] Which one of the following is a plant hormone?
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Cytokinins stimulate cell division
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trans-Zeatin | BDOSHOP.com | Various Chemical Solutions | CHEM-LAB-TECH Supply
Greater understanding of plant hormone results in stem cells that grow shoots - Scienmag: Latest Science and Health News
trans-Zeatin glucoside - Alfa Chemistry
CAS 525-79-1 Cytokinin Plant Growth Regulator 6 - Furfurylaminopurine 98% TC
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Real-time PCR Detection of <i>Rhodococcus fascians</i> and Discovery of New Plants Associated with <i>R. fascians...
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The Discovery of Cytokinin. - The Quiet Branches
Plant hormones with overlapping signaling pathways play distinct roles in controlling growth
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Plant hormone
Cytokinins or CKs are a group of chemicals that influence cell division and shoot formation. They were called kinins in the ... Cytokinins and auxins often work together, and the ratios of these two groups of plant hormones affect most major growth ... Sipes DL, Einset JW (August 1983). "Cytokinin stimulation of abscission in lemon pistil explants". J Plant Growth Regul. 2 (1-3 ... Cytokinins counter the apical dominance induced by auxins; they in conjunction with ethylene promote abscission of leaves, ...
Sporulation in Bacillus subtilis
Similarly, in Dictyostelium discoideum (slime mold), SDF-2 and cytokinins are secreted during late development to trigger ... Anjard, C.; Loomis, W. F. (2008). "Cytokinins induce sporulation in Dictyostelium". Development. 135 (5): 819-27. doi:10.1242/ ...
Coconut milk
Although there are many factors that attribute coconut milk to plant growth, the main cause is the existence of a cytokinin ... Mok, David W. S.; Mok, Machteld C. (1994). Cytokinins: Chemistry, Activity, and Function. CRC Press. ISBN 0-8493-6252-0. Bajaj ...
Ethylene as a plant hormone
Auxins Cytokinins IAA Growth substances, Ethylene. 7: 231-248. Yang SF, Hoffman NE (1984). "Ethylene biosynthesis and its ... ACC synthesis increases with high levels of auxins, especially indole acetic acid (IAA) and cytokinins. Ethylene is perceived ...
Kinetin
1994). Cytokinins: chemistry, activity and function. Boca Raton, Florida: CRC Press. ISBN 978-0-8493-6252-1. Barciszewski, J.; ... Kinetin (/'kaɪnɪtɪn/) is a type of cytokinin, a class of plant hormone that promotes cell division. Kinetin was originally ...
Peter K. Hepler
and cytokinin action. Hepler's research is currently aimed at finding the ionic and molecular components that make up the ... Saunders, M. J.; P. K. Hepler (1982). "Calcium ionophore A23187 stimulates cytokinin-like mitosis in Funaria". Science. 217 ( ... Saunders, M. J.; P. K. Hepler (1981). "Localization of membrane-associated calcium following cytokinin treatment in Funaria ...
Zeatin 9-aminocarboxyethyltransferase
Other names in common use include beta-(9-cytokinin)-alanine synthase, beta-(9-cytokinin)alanine synthase, O-acetyl-L-serine ... doi:10.1016/0031-9422(83)83008-8. Mok DWS; Mok MC (2001). "Cytokinin metabolism and action". Annu. Rev. Plant Physiol. Plant ... Entsch B, Parker CW, Letham DS (1983). "An enzyme from lupin seeds forming alanine derivatives of cytokinins". Phytochemistry. ...
Gynura procumbens
... for cytokinin-like compounds". Bioinformation. 5 (5): 191-197. doi:10.6026/97320630005191. ISSN 0973-2063. PMC 3040498. PMID ...
Plant hormone
Cytokinins or CKs are a group of chemicals that influence cell division and shoot formation. They also help delay senescence of ... Cytokinins and auxins often work together, and the ratios of these two groups of plant hormones affect most major growth ... Cytokinins counter the apical dominance induced by auxins; in conjunction with ethylene, they promote abscission of leaves, ... They also promote the production of other hormones and, in conjunction with cytokinins, control the growth of stems, roots, and ...
Symbiotic bacteria
In this connection, cytokinins have been found to play a role in the development of root fixing nodules. It appears that not ... Frugier F, Kosuta S, Murray JD, Crespi M, Szczyglowski K (March 2008). "Cytokinin: secret agent of symbiosis". Trends in Plant ... only must the plant have a need for nitrogen fixing bacteria, but they must also be able to synthesize cytokinins which promote ...
Agrobacterium tumefaciens
Genes for the production of cytokinins are also expressed. This stimulates cell proliferation and gall formation. The T-DNA ...
Gaseous signaling molecules
Auxins Cytokinins IAA Growth substances, Ethylene Yang, S. F.; Hoffman N. E. (1984). "Ethylene biosynthesis and its regulation ... ACC synthesis increases with high levels of auxins, especially indole acetic acid (IAA) and cytokinins. Ethylene is perceived ...
Auxin
When auxin and cytokinin are applied to callus, rooting can be generated with higher auxin to cytokinin ratios, shoot growth is ... For example, the ratio of auxin to cytokinin in certain plant tissues determines initiation of root versus shoot buds. On the ... ALONI, R.; ALONI, E.; LANGHANS, M.; ULLRICH, C. I. (May 2006). "Role of Cytokinin and Auxin in Shaping Root Architecture: ... Auxin also stimulates cell division if cytokinins are present. ... such as strigolactones or cytokinins) in the process on various ...
Ectomycorrhiza
2013). "Cytokinins as key regulators in plant-microbe-insect interactions: connecting plant growth and defence" (PDF). ... They can also increase root branching by inducing cytokinins in the plant. These branching patterns can become so extensive ... These include flavonoids, diterpenes, cytokinins, hormones and other nutrients. Some host-released metabolites have been shown ...
Biological functions of nitric oxide
Liu, W. Z.; Kong, D. D.; Gu, X. X.; Gao, H. B.; Wang, J. Z.; Xia, M.; He, Y. K. (2013). "Cytokinins can act as suppressors of ... Abscisic acid and cytokinin. Atmospheric nitric oxide can enter the stomates of most vascular species, and can have effects ...
Hydrotropism
Furthermore, we have gathered that cytokinins also play a crucial role. This is interesting because cytokinin works ... The cytokinins cause the degradation of the auxin transporting PIN1 proteins, which prevents auxin from accumulating in the ... The nhr1 plants would begin to show hydrotropic response only in the presence of kinetin, which is a type of cytokinin. This ... mutant of Arabidopsis recovers root hydrotropism with cytokinin". Journal of Experimental Botany. 63 (10): 3587-3601. doi: ...
Meristem
Cytokinin signaling is positively reinforced by WUS to prevent the inhibition of cytokinin signaling, while WUS promotes its ... The function of WUS in the shoot apical meristem is linked to the phytohormone cytokinin. Cytokinin activates histidine kinases ... Type-B ARRs work as transcription factors to activate genes downstream of cytokinin, including A-ARRs. A-ARRs are similar to B- ... As a result, B-ARRs are no longer inhibited, causing sustained cytokinin signaling in the center of the shoot apical meristem. ...
Taphrina caerulescens
Taphrina species have been shown to produce cytokinin. A layer of asci emerge within the lesions on the bottom of the leaf. ...
Methylobacterium extorquens
LPSN lpsn.dsmz.de Lidstrom, Mary E.; Chistoserdova, Ludmila (2002-04-01). "Plants in the Pink: Cytokinin Production by ...
Auxin
When auxin and cytokinin are applied to callus, rooting can be generated with higher auxin to cytokinin ratios, shoot growth is ... Crown galls are caused by Agrobacterium tumefaciens bacteria; they produce and excrete auxin and cytokinin, which interfere ... For example, the ratio of auxin to cytokinin in certain plant tissues determines initiation of root versus shoot buds. ... Auxin also stimulates cell division if cytokinins are present. ... such as strigolactones or cytokinins) in the process on various ...
Plant senescence
Cytokinins help to maintain the plant cell and expression of cytokinin biosynthesis genes late in development prevents leaf ... A withdrawal of or inability of the cell to perceive cytokinin may cause it to undergo apoptosis or senescence. In addition, ... Ivanova M., and Rost T. L., (1998). "Cytokinins and the plant cell cycle: Problems and pitfalls of proving their function". ... Parallels to cell division - the theory, perhaps even more controversially, asserts that just as both auxin and cytokinin seem ...
Hormone
Examples include abscisic acid, auxin, cytokinin, ethylene, and gibberellin. Most hormones initiate a cellular response by ...
Chemoreceptor
These include auxin, abscisic acid, gibberellin, cytokinin, and ethylene. Once bound, hormones can induce, inhibit, or maintain ...
Phloroglucinol
It demonstrates both cytokinin-like and auxin-like activity. Phloroglucinol increases shoot formation and somatic embryogenesis ...
1,3-Diphenylurea
DPU is a cytokinin, a type of plant hormone that induces flower development. It occurs in coconut milk. The cytokinin effect of ... Effect of cytokinin-active phenylurea derivatives on shoot multiplication. T. Genkov and I. Ivanova, Bulg. J. Plant Physiol., ... DPU is relatively low, but other more potent phenylurea-type cytokinins have been reported. Shantz, E. M.; Steward, F. C. (1955 ...
Agar
Birgit Hadeler; Sirkka Scholz; Ralf Reski (1995). "Gelrite and agar differently influence cytokinin-sensitivity of a moss". ...
Flower induction
Biochemical changes at the apex, particularly those caused by cytokinins, accompany this process. Usually flower induction is ...
Callus (cell biology)
Specific auxin to cytokinin ratios in plant tissue culture medium give rise to an unorganized growing and dividing mass of ... Beutelmann, P.; Bauer, L. (1 January 1977). "Purification and identification of a cytokinin from moss callus cells". Planta. ... cytokinin, and gibberellin, to initiate callus formation or somatic embryogenesis. Callus initiation has been described for all ...
Growth medium
ISBN 0-13-144329-1. Birgit Hadeler, Sirkka Scholz, Ralf Reski (1995). "Gelrite and agar differently influence cytokinin- ...
Root
Cytokinins - Cytokinins regulate root apical meristem size and promote lateral root elongation. ... In response to the concentration of nutrients, roots also synthesise cytokinin, which acts as a signal as to how fast the ...
Cytokinin -- Britannica Online Encyclopedia
Cytokinins
... are N (6) substituted adenine derivatives that affect many aspects of plant growth and development, including cell ... and how other signaling pathways influence cytokinin levels and sensitivity though modulation of the expression of cytokinin ... Cytokinins Arabidopsis Book. 2014 Jan 2;12:e0168. doi: 10.1199/tab.0168. ... Cytokinins are N (6) substituted adenine derivatives that affect many aspects of plant growth and development, including cell ...
Perception and signal transduction of cytokinins. - PubMed - NCBI
... and key genes for the metabolism and actions of cytokinins have recently been identified. Cytokinins are perceived by three ... Perception and signal transduction of cytokinins.. Kakimoto T1.. Author information. 1. Department of Biology, Graduate School ... Cytokinins are plant hormones implicated in diverse and essential processes in plant growth and development, ... On the other hand, the type-A ARRs act as repressors of cytokinin-activated transcription. How phosphorelay regulate response ...
Cytokinin - Wikipedia
The ratio of auxin to cytokinin plays an important role in the effect of cytokinin on plant growth. Cytokinin alone has no ... Cytokinin signaling in plants is mediated by a two-component phosphorelay. This pathway is initiated by cytokinin binding to a ... Cytokinins have recently been found to play a role in plant pathogenesis. For example, cytokinins have been described to induce ... When cytokinin is added, the cells expand and differentiate. When cytokinin and auxin are present in equal levels, the ...
Cytokinins | SpringerLink
The first cytokinin was discovered from Herring (an oily fish from genus Clupea) sperm DNA by Miller et al. in... ... Cytokinins (CK) are a class of plant growth substances which promote cell division. ... Cytokinins (CK) are a class of plant growth substances which promote cell division. The first cytokinin was discovered from ... Osugi A, Sakakibara H (2015) Q&A: how do plants respond to cytokinins and what is their importance? BMC Biol 13:102CrossRef ...
cytokinin antibodies
... Stuart Baum sfbaum at UCDAVIS.EDU Wed Apr 5 14:32:32 EST 1995 *Previous message: Rattling the weed (with ... Also, I would be interested to hear any comments concerning the use of antibodies to locate cytokinins in plant tissues. Thanks ... Does anyone have information regarding acquisition of antibodies against cytokinins? These will be used for immunolocalization ...
Cytokinin dehydrogenase - Wikipedia
Galuszka P, Frébort I, Šebela M, Sauer P, Jacobsen S, Peč P (2001). "Cytokinin oxidase or dehydrogenase? Mechanism of cytokinin ... In enzymology, a cytokinin dehydrogenase (EC 1.5.99.12) is an enzyme that catalyzes the chemical reaction N6- ... and cytokinin oxidase/dehydrogenase abbreviated as CKX. As of late 2007, 6 structures have been solved for this class of ... reduced acceptor The 3 substrates of this enzyme are cytokinin (here represented by N6-dimethylallyladenine), electron acceptor ...
The role of cytokinins in clubroot disease | SpringerLink
The activities of cytokinin glucosides and alanine conjugates in cytokinin bioassays. Journal of Plant Growth Regulation, 2(1-4 ... The cytokinin content of control and clubroot-infected galls. The complex nature of cytokinin biosynthesis, conjugation and ... The cytokinin content of control and clubroot-infected Arabidopsis plants. Active, precursor and conjugated cytokinin content ... The impact of pathogen-derived cytokinins on the total cytokinin content of infected tissue and host gene expression was ...
Patent US20090317863 - Use of active cytokinin synthase gene - Google Patents
... regulating the amount of active cytokinins synthesized and to provide a transformed plant with a regulated amount of cytokinins ... 15 and encodes a protein having activity of catalyzing reactions that synthesize active cytokinins from nucleotide cytokinins ... amino acid residues and has activity of catalyzing reactions that synthesize active cytokinins from nucleotide cytokinins. ... has had a function to catalyze reactions that synthesize active cytokinins from nucleotide cytokinins (a cytokinin activation ...
Arabidopsis Cytokinin Signaling Pathway | Science Signaling
Elucidating the Cytokinin Pathway | Science Signaling
... with and without added cytokinin and measured the expression of a cytokinin-responsive reporter gene. The CKI1 sensor is ... The ability of CKI1 and ARR2 to promote cytokinin-mediated phenotypes was confirmed in transfected plants. The cytokinin ... I. Hwang, J. Sheen, Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature 413, 383-389 (2001). [Online ... Expression of the A-type ARR proteins resulted in inhibition of the cytokinin-stimulated reporter gene activity, and expression ...
Investigation of cytokinin receptor from maize
Our research is open for cooperation and joint studies of the mechanisms of cytokinin reception and cytokinin signal ... Investigation of cytokinin receptor from maize. Feodor A. Brovko brovko at fibkh.serpukhov.su Wed Jul 1 22:17:50 EST 1998 * ... Dear Colleagues: Will you be so kind to help us with pursuing the research of the cytokinin receptor from maize? Previously, we ... We think that elucidation of the correlation between the content of cytokinins in tissue (or in the cell) and the content, ...
Cytokinins: metabolism and function in plant adaptation to environmental stresses. - PubMed - NCBI
Cytokinin production by species of the fungus Taphrina | Open Library
Cytokinin Receptor Antagonists and Compositions Containing these Derivatives
... purin derivatives as cytokinin re-ceptor antagonists to provide cytokinin analogons for growth regulation in plants and to ... Cytokinins are plant hormones involved in cell division, shoot meristem and leaf formation, chloroplast biogenesis, and ... The potent naturally occurring cytokinin N6-isopentenyladenine served as the basis for initial structure-activity studies. ,/p ... offer the possibility for a specific selectivity for cytokinin receptors without being toxic for animal cells. The derivatives ...
Cytokinin signaling in plant development | Development
Cytokinin transport. It was originally thought that cytokinins were synthesized in the roots and then transported to the shoots ... Cytokinin functions in plant development. Cytokinins play many roles in plant development, often acting in concert with other ... 2016). Cytokinin response factors integrate auxin and cytokinin pathways for female reproductive organ development. Development ... Cytokinin levels in plants are regulated by biosynthesis and inactivation pathways. Cytokinins are perceived by membrane- ...
Integration of epigenetic and genetic controls of seed size by cytokinin in Arabidopsis | PNAS
Here, we identify a target of the IKU pathway, the cytokinin oxidase CKX2, that affects cytokinin signaling. CKX2 expression is ... To monitor the level of cytokinin activity in vivo, we used a cytokinin reporter construct that consists of an artificial ... a member of a family of enzymes involved in catabolism of the plant hormone cytokinin (29). Cytokinin controls growth and cell ... The high level of cytokinin activity found in endosperm suggested that the plant hormone cytokinin regulates growth of seed ...
Cytokinin production by Agrobacterium tumefaciens
The first step of purification utilized the adsorption of cytokinins onto C18 silica by a process known as trace enrichment. ... In view of the fragmentary and presumptive evidence available on the quantitative and qualitative levels of cytokinins produced ... Possible mechanisms for the production of these cytokinins were discussed. Preliminary evidence on three strains of A. ... Further purification was effected by high performance liquid chromatography and cytokinin-active fractions were located by ...
Cytokinin for relief from food crises, moss for environmental remediation
"Cytokinin oxidase 2 is expressed in the flower buds and degrades cytokinin. We found that the expression level of cytokinin ... Controlling cytokinin. If cytokinin biosynthesis or activation is artificially adjustable, it might also be possible to improve ... "Little had been known about cytokinin activation. Although science textbooks taught that cytokinin becomes activated in two ... Is it therefore possible to control the biosynthesis, activation, and degradation of cytokinin? "Cytokinin is structurally a ...
ARR1, a Transcription Factor for Genes Immediately Responsive to Cytokinins | Science
These cytokinin responses in Arabidopsis are at least partly triggered through the recognition of cytokinins by the sensor ... Residual cytokinin responses observed with the arr1-1 mutant may have been provided by ARR2. In addition to ARR6, other type A ... Cytokinins are a class of phytohormones involved in various physiological events of plants. The Arabidopsis sensor histidine ... In Arabidopsis, cytokinin receptors, ethylene receptors, and, possibly, osmosensors are histidine kinases (3, 4, 23-25). ...
Cytokinin Signaling in Mycobacterium tuberculosis | mBio
Cytokinin Signaling in Mycobacterium tuberculosis. Marie I. Samanovic, Hao-Chi Hsu, Marcus B. Jones, Victoria Jones, Michael R. ... Cytokinin Signaling in Mycobacterium tuberculosis. Marie I. Samanovic, Hao-Chi Hsu, Marcus B. Jones, Victoria Jones, Michael R. ... Cytokinin Signaling in Mycobacterium tuberculosis. Marie I. Samanovic, Hao-Chi Hsu, Marcus B. Jones, Victoria Jones, Michael R ... Here, we show cytokinins induced the strong expression of the M. tuberculosis gene Rv0077c. We found that Rv0077c expression is ...
CKI1, a Histidine Kinase Homolog Implicated in Cytokinin Signal Transduction | Science
Although cytokinin plays a central role in plant development, little is known about cytokinin signal transduction. Five ... These results suggest that CKI1 is involved in cytokinin signal transduction, possibly as a cytokinin receptor. ... CKI1, a Histidine Kinase Homolog Implicated in Cytokinin Signal Transduction Message Subject. (Your Name) has forwarded a page ... A gene, CKI1, which was tagged in four of the five mutants and induced typical cytokinin responses after introduction and ...
Mass Spectrometric Analysis of Cytokinins in Plant Tissues | Plant Physiology
Mass Spectrometric Analysis of Cytokinins in Plant Tissues. V. Identification of the Cytokinin Complex of Datura Innoxia Crown ... The cytokinin complex of Datura innoxia Mill. crown gall tissue was purified by ion exchange, Sephadex LH-20 chromatography and ... Mass Spectrometric Analysis of Cytokinins in Plant Tissues Message Subject (Your Name) has sent you a message from Plant ... By gas chromatography-mass spectrometry using 2H-labeled compounds, the following cytokinins were identified in the basic ...
Plant Growth Regulators - Auxins, Gibberellins, Cytokinins, Kinetin, Abscisic & Gibberellic Acid
Crossing paths: cytokinin signalling and crosstalk | Development
Cytokinin and its biosynthesis. (A) Common cytokinins found in plants include the isoprenoid cytokinins trans-zeatin (tz) and ... The effect of cytokinin on PIN1 was reduced in the cre1 and cre1 ahk2 cytokinin receptor mutants, confirming that this ... Histidine kinases: the cytokinin receptors. The first indication of a link between cytokinin and the bacterial TCS was the ... Reduction of cytokinin signalling in the cytokinin receptor mutants or by induction of CKI expression in the vascular domain ...
Cytokinin Targets Auxin Transport to Promote Shoot Branching | Plant Physiology
Cytokinin Targets Auxin Transport to Promote Shoot Branching Message Subject (Your Name) has sent you a message from Plant ... Cytokinin promotes the accumulation of PIN3-GFP, PIN4-GFP, and PIN7-GFP on the basal plasma membrane of xylem parenchyma cells ... arr1 buds have wild-type responses to auxin and cytokinin but are resistant to strigolactone. Wild-type (A and C) or arr1 (B ... Cytokinin Targets Auxin Transport to Promote Shoot Branching. Tanya Waldie, Ottoline Leyser ...
Cytokinin inhibits a subset of diageotropica-dependent primary auxin responses in tomato | RTI
To test whether cytokinin controls plant development through inhibiting an early step in the auxin response pathway, we ... Many aspects of plant development are regulated by antagonistic interactions between the plant hormones auxin and cytokinin, ... compared the effects of cytokinin with those of the dgt ... However, cytokinin failed to inhibit auxin-induced LeSAUR gene ... Thus, cytokinin inhibited a subset of the auxin responses impaired in dgt hypocotyls, suggesting that cytokinin blocks at least ...
Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells
Abscisic Acid-Cytokinin Antagonism Modulates Resistance Against Pseudomonas syringae in Tobacco
... Dominik K. Großkinsky, Eric ... Although interactions of cytokinins with salicylic acid and auxin have been indicated, the complete network of cytokinin ... Abscisic Acid-Cytokinin Antagonism Modulates Resistance Against Pseudomonas syringae in Tobacco. December 2014 , Volume 104 , ... Only recently, a function of the classic phytohormone cytokinin in plant immunity has been described in Arabidopsis, rice, and ...
BiosynthesisKinetinMetabolismZeatinAdenineOxidaseGenesAbstractEthyleneSynthetic cytokininHormonesReceptorPhytohormonesGeneAbscisic acidRegulateBiosyntheticPathwaysTissuesIsoprenoidResponsesRole of cytokininAuxins and CytokininsAnalysis of CytokininsSignalingTranscriptionalEndogenous cytokininsRegulatesEnzymesMutantsSignal transduction in plantReceptorsRegulatorsCRE1Effect of cytokininGibberellinSynthesisDegradationBudsLateralInhibitHistidine kinasesLevels of cytokininsMolecularSenescenceRootsTotal cytokininInteractionsXylemInduceAntibodiesARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEINMechanismCrosstalkType of cytokininShown that cytokininEffects of cytokininContent of cytokinins
Biosynthesis22
- Here, we review our current understanding of cytokinin biosynthesis and signaling in Arabidopsis, the latter of which is similar to bacterial two-component phosphorelays. (nih.gov)
- is the major site of cytokinin biosynthesis. (springer.com)
- Adenosine phosphate-isopentenyltransferase (IPT) catalyses the first reaction in the biosynthesis of isoprene cytokinins. (wikipedia.org)
- This reaction is the rate-limiting step in cytokinin biosynthesis. (wikipedia.org)
- DMADP and HMBDP used in cytokinin biosynthesis are produced by the methylerythritol phosphate pathway (MEP). (wikipedia.org)
- Auxin is known to regulate the biosynthesis of cytokinin. (wikipedia.org)
- Expression of host genes associated with cytokinin biosynthesis, signalling, degradation and conjugation was strongly repressed. (springer.com)
- Two isopentenyltransferase genes associated with cytokinin biosynthesis are present in the P. brassicae genome and are expressed throughout gall formation. (springer.com)
- Cytokinin levels in plants are regulated by biosynthesis and inactivation pathways. (biologists.org)
- The biosynthesis of cytokinins begins with the addition of a prenyl group derived from dimethylallyl diphosphate to the N 6 position of ADP/ATP. (biologists.org)
- The Biodynamics Research Team at RIKEN leads the world in cytokinin research and are working to control the biosynthesis and activation of cytokinin with the aim of increasing the yields of rice, wheat, corn, and other crops. (innovations-report.com)
- Cytokinin and its biosynthesis. (biologists.org)
- B ) The cytokinin biosynthesis pathway, highlighting the key enzymes involved. (biologists.org)
- Cytokinin biosynthesis is a multi-step process requiring the activity of several different genes. (biologists.org)
- In Arabidopsis thaliana , the model species for plant research, cytokinin biosynthesis is controlled by the ATP/ADP isopentenyltransferase (IPT) genes, which encode rate-limiting enzymes in cytokinin biosynthesis ( Fig. 1B ). (biologists.org)
- Chen CM (1981) Biosynthesis and enzymic regulation of the interconversion of cytokinin. (springer.com)
- trans -Zeatin cytokinin biosynthesis and the CRE1/AHK2 receptor combination are crucial for P. indica --mediated growth stimulation, while mutants lacking cis -zeatin, impaired in other cytokinin receptor combinations, or containing reduced cytokinin levels respond to the fungus. (apsnet.org)
- however, it may not be involved in the biosynthesis of this minor cytokinin in vivo. (uniprot.org)
- Cytokinin metabolism is composed of biosynthesis, degradation and modification processes, requiring the activity of several key enzymes. (biomedcentral.com)
- For instance, Isopentenyltransferase (IPT) catalyses the first reaction in the biosynthesis of isoprene cytokinins, isopentenyladenine-5′-monophosphate [ 13 ]. (biomedcentral.com)
- The bacterium does not produce these phytohor-mones itself, but transfers the genes for the biosynthesis of cytokinin and auxin from its T i plasmid to the plant genome. (brainkart.com)
- Here, to gain insight into this elaborate signaling system, we summarize current models of biosynthesis, trans-membrane transport, and long-distance translocation of cytokinins in higher plants. (usda.gov)
Kinetin9
- There are two types of cytokinins: adenine-type cytokinins represented by kinetin, zeatin, and 6-benzylaminopurine, and phenylurea-type cytokinins like diphenylurea and thidiazuron (TDZ). (wikipedia.org)
- Other cytokinins, including kinetin and zeatin when applied at 10-4M would also induce adventitious bud formation, while N6-isopentenylpurine (2iP) even when applied at 10-3M did not. (oregonstate.edu)
- Other cytokinins including kinetin, zeatin and 6-benzyl-9-tetrahydropyrone adenine (SD 8339) were all able to induce adventitious bud formation when applied at 1O-5M, however, 2iP induced bud formation only when applied at 10-4M. (oregonstate.edu)
- Cytokinins are plant growth factors that regulate cell growth and differentiation in plants.1 Kinetin (N6-furfuryladenine) is a cytokinin growth factor that has been shown to (1) delay age-related changes in morphological and biochemical characteristics of human skin fibroblasts 2 and keratinocytes, 3 (2) inhibit oxidative and glycoxidative damage to proteins in vitro, 4 (3) protect DNA against oxidative damage, 5 and (4) scavenge free radicals. (jddonline.com)
- Adenine type of cytokinin includes zeatin, kinetin and 6-benzylaminopurine while phenylurea type of cytokinin includes thidiazuron and diphenylurea. (researchnester.com)
- Cytokinin hormones, such as kinetin, is used commercially to reduce yellowing in vegetables and maintain long-term storage. (doraagri.com)
- One particular cytokinin, called kinetin, had anti-aging effects on human skin cells and increased the life span of fruit flies. (livestrong.com)
- This year is the 60th anniversary of the discovery of the first cytokinin, kinetin. (thequietbranches.com)
- The first cytokinin, Kinetin. (thequietbranches.com)
Metabolism13
- The identification and genetic manipulation of the genes involved in cytokinin metabolism and signaling have helped illuminate the roles of cytokinins in Arabidopsis. (nih.gov)
- Cytokinins are plant hormones implicated in diverse and essential processes in plant growth and development, and key genes for the metabolism and actions of cytokinins have recently been identified. (nih.gov)
- This paper uses transcriptomics and metabolomics to investigate changes in cytokinin metabolism during gall formation of clubroot-infected Arabidopsis thaliana . (springer.com)
- RNASeq analysis of infected tissue showed that host cytokinin metabolism was strongly down-regulated both at the onset and late stages of gall formation. (springer.com)
- Cytokinins: metabolism and function in plant adaptation to environmental stresses. (nih.gov)
- Here, and in the accompanying poster, we summarize the current understanding of cytokinin metabolism, transport and signaling, and discuss how this phytohormone regulates changes in gene expression to mediate its pleiotropic effects. (biologists.org)
- In: Guern J, Péaud-Lenoël C (eds) Metabolism and molecular activities of cytokinins. (springer.com)
- Chism GW III, Long AR, Rolle R (1984) Assay of enzymes involved in cytokinin metabolism by means of reversed-phase high-performance liquid chromatography. (springer.com)
- Laloue M, Pethe C (1982) Dynamics of cytokinin metabolism in tobacco cells. (springer.com)
- Vitti A, Nuzzaci M, Scopa A, Tataranni G, Remans T, Vangronsveld J, Sofo A. Auxin and Cytokinin Metabolism and Root Morphological Modifications in Arabidopsis thaliana Seedlings Infected with Cucumber mosaic virus (CMV) or Exposed to Cadmium. (mdpi.com)
- Our results suggest that DELLA proteins may regulate cytokinin metabolism upon nodulation. (edu.au)
- Cytokinins: Metabolism and Biological Activity of N6-(Δ2-Isope. (mysciencework.com)
- This cytokinin-dependent communication system consists of orchestrated regulation of the metabolism, translocation, and signal transduction of this phytohormone class. (usda.gov)
Zeatin14
- Zeatin was also the first natural cytokinin reported from unripe maize kernels by Miller and Letham in 1963. (springer.com)
- 2. Zeatin-binding proteins participating in cytokinin-dependent activation of transcripton. (bio.net)
- 1955 ), and subsequent studies identified the cytokinin zeatin as an endogenous plant growth regulator ( Letham, 1973 ). (biologists.org)
- In higher plants, the most prevalent cytokinin is zeatin, which can occur in both trans and cis configurations. (biologists.org)
- By gas chromatography-mass spectrometry using 2 H-labeled compounds, the following cytokinins were identified in the basic fraction eluting from a cation exchange column: zeatin, zeatin riboside, dihydrozeatin, dihydrozeatin riboside, their corresponding O -glucosides, 7- and 9-glucosides of zeatin, 9-glucoside of dihydrozeatin, isopentenyladenine, and isopentenyladenosine. (plantphysiol.org)
- Zeatin riboside 5′-monophosphate was the major cytokinin nucleotide in the tissue. (plantphysiol.org)
- this was followed by the identification of trans-zeatin ( Miller, 1961 ) and many other cytokinins (reviewed in Mok and Mok, 2001 ). (biologists.org)
- A ) Common cytokinins found in plants include the isoprenoid cytokinins trans-zeatin (tz) and isopentenyl-adenine (iP). (biologists.org)
- A set of 11 relevant isoprenoid cytokinins, including zeatin isomers, was separated by ultra performance liquid chromatography in less than 6 min. (diva-portal.org)
- The contents of endogenous indoleacetic acid (IAA) and five cytokinins (Cks), N 6 (2-isopentenyl)adenine (iP), N 6 (2-isopentenyl)adenosine (iPR), zeatin (Z), zeatin riboside (ZR) and N 6 -benzyladenine (BA), present in the basal portion of those leaves, were correlated to the organogenic response that occurs over 15 days of culture. (scielo.br)
- In zeatin, which is the most common cytokinin, the amino group of adenine is linked with the hydroxylated isoprene residue in the trans-position. (brainkart.com)
- Cytokinin - The cytokinin zeatin is named after the genus of corn, Zea, in which it was discovered. (enacademic.com)
- C ytokinins (CK) belong to a class of plant growth substances (plant hormones) which promote cell deivision by means of local and long distance signalling.Zeatin is an adenine-type cytokinin synthesised in stems, leaves and roots. (agrisera.com)
- Zeatin is the natural cytokinin which was first isolated from Maize and it is the most common cytokinin. (lifeeasy.org)
Adenine16
- Cytokinin , any of a number of plant growth substances that are usually derived from adenine. (britannica.com)
- Most adenine-type cytokinins are synthesized in roots. (wikipedia.org)
- In enzymology, a cytokinin dehydrogenase (EC 1.5.99.12) is an enzyme that catalyzes the chemical reaction N6-dimethylallyladenine + electron acceptor + H2O ⇌ {\displaystyle \rightleftharpoons } adenine + 3-methylbut-2-enal + reduced acceptor The 3 substrates of this enzyme are cytokinin (here represented by N6-dimethylallyladenine), electron acceptor, and H2O, whereas its 3 products are adenine, 3-methylbut-2-enal (or other aldehyde in case of different substrate), and reduced acceptor. (wikipedia.org)
- Naturally occurring cytokinins are adenine derivatives with isoprenoid side chains attached to the N 6 position of the adenine ring. (biologists.org)
- While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life. (asm.org)
- Cytokinins, which were named for their ability to promote cytokinesis, are a class of phytohormone derived from adenine. (biologists.org)
- CKX (cytokinin dehydrogenase) is a flavoprotein that cleaves cytokinins to adenine and the corresponding side-chain aldehyde using a quinone-type electron acceptor. (portlandpress.com)
- The imine product only very slowly hydrolyses to adenine and an aldehyde derived from the cytokinin N 6 side-chain. (portlandpress.com)
- The cytokinin, N6-(Δ2-isopentenyl)adenine, is found to be at least 3.3 times as active as N6-(Δ2-isopentenyl)adenosine in promoting the growth of cytokinin-requiring tobacco (Nicotiana tabacum) callus. (mysciencework.com)
- Thus, it is suggested that N6-(Δ2-isopentenyl)adenine-7-glucoside is a protected or storage form of the cytokinin which could account for the higher biological activity of N6-(Δ2-isopentenyl)adenine than of N6-(Δ2-isopentenyl)adenosine. (mysciencework.com)
- Cytokinins are prenylated derivatives of adenine. (brainkart.com)
- In other cytokinins benzyl derivatives, sugars or sugar phosphates are attached to the adenine. (brainkart.com)
- The chemical reactions and pathways involving cytokinins, a class of adenine-derived compounds that can function in plants as growth regulators. (systemsbiology.net)
- The hypothesis arose from the observation that a compound used to identify adenine binding RNA sequences is chemically similar to the classic plant hormone, or growth regulator, cytokinin. (biomedcentral.com)
- The hypothesis is plausible, as demonstrated by a cursory review of the transcriptome and genome of the model plant Arabidopsis thaliana for transcripts that i) contain an adenine aptamer motif, and ii) are also predicted to be cytokinin-regulated. (biomedcentral.com)
- Here, one gene, CRK10 (for Cysteine-rich Receptor-like Kinase 10 , At4g23180), contains an adenine aptamer-related sequence and is down-regulated by cytokinin approximately three-fold in public gene expression data. (biomedcentral.com)
Oxidase9
- For example, plants constitutively over-expressing cytokinin oxidase show reduced gall formation (Siemens et al. (springer.com)
- Other names in common use include N6-dimethylallyladenine:(acceptor) oxidoreductase, 6-N-dimethylallyladenine:acceptor oxidoreductase, and cytokinin oxidase/dehydrogenase abbreviated as CKX. (wikipedia.org)
- Cytokinin oxidase or dehydrogenase? (wikipedia.org)
- Here, we identify a target of the IKU pathway, the cytokinin oxidase CKX2, that affects cytokinin signaling. (pnas.org)
- Cytokinin oxidase/dehydrogenase genes in barley and wheat: cloning and heterologous expression. (sigmaaldrich.com)
- The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described in this work. (sigmaaldrich.com)
- The catalytic reaction of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) was studied in detail using the recombinant flavoenzyme from maize. (portlandpress.com)
- Restriction map and gene structure of maizeckx1 and gene structures of seven Arabidopsis cytokinin oxidase homologs. (mysciencework.com)
- Expression of recombinant maize cytokinin oxidase. (mysciencework.com)
Genes11
- We discuss these diverse roles, and how other signaling pathways influence cytokinin levels and sensitivity though modulation of the expression of cytokinin signaling and metabolic genes. (nih.gov)
- At later stages of gall formation the expression of host cytokinin biosynthetic genes is repressed, but so too is the expression of host cytokinin oxidases and dehydrogenases (Devos et al. (springer.com)
- The remainder (type A) possess no apparent functional unit other than a signal receiver domain containing two aspartate and one lysine residues (DDK) at invariant positions, and their genes are transcriptionally induced by cytokinins without de novo protein synthesis ( 7 , 9 , 10 ). (sciencemag.org)
- In addition, cytokinin treatment inhibited the auxin induction of only one of two 1-aminocyclopropane-1-carboxylic acid synthase genes that exhibited impaired auxin inducibility in dgt hypocotyls. (rti.org)
- Transformation of both genes into Arabidopsis and tobacco showed that at least one of the genes codes for a functional enzyme, as its expression caused a cytokinin-deficient phenotype in the heterologous host plants. (sigmaaldrich.com)
- Overexpression of Cytokinin Dehydrogenase Genes in Barley (Hordeum vulgare cv. (plos.org)
- Here, we show in Pisum sativum L. that the DELLA proteins can activate the expression of KNOX and BELL transcription factors involved in regulation of cytokinin metabolic and response genes. (edu.au)
- Consistently, pea la cry-s (della1 della2) mutant showed reduced ability to upregulate expression of some cytokinin metabolic genes during nodulation. (edu.au)
- In this study, via analyzing the expression of cyclin genes during lateral root (LR) formation, we report that cytokinins (CTKs) inhibit the initiation of LR through blocking the pericycle founder cells cycling at the G 2 to M transition phase, while the promotion by CTK of LR elongation is due to the stimulation of the G 1 to S transition. (oup.com)
- In particular, we demonstrate that DELLA proteins are recruited by type-B ARABIDOPSIS RESPONSE REGULATORS (ARR) to the promoters of cytokinin-regulated genes, where they act as transcriptional co-activators. (upv.es)
- LUC fusion genes showed that both the ARR15 and the ARR16 gene products are accumulated upon cytokinin treatment in roots. (oup.com)
Abstract1
- abstract = "Primary crown gall tumours induced on stems on tomato plants by Agrobacterium tumefaciens yielded extracts with pronounced cytokinin activity. (edu.au)
Ethylene7
- Cytokinin treatment also inhibited rapid auxin responses in hypocotyl segments: auxin-stimulated elongation, H(+) secretion, and ethylene synthesis were all inhibited by cytokinin in wild-type hypocotyl segments, and thus mimicked the impaired auxin responsiveness found in dgt hypocotyls. (rti.org)
- Finally, putative direct and indirect targets of the constitutively active PtRR13 transcription factor imply that cross-talk between cytokinin, auxin and ethylene are important during adventitious rooting in cuttings. (ufl.edu)
- Ethylene production was higher in cultures supplemented with cytokinins (especially with meta-topolin), with high sprouting percentages, and lenticel hypertrophy. (brillonline.com)
- Here we define key mechanistic elements in a regulatory network by which cytokinin inhibits root cell elongation in concert with the hormones auxin and ethylene. (nottingham.ac.uk)
- Cytokinin regulates root cell elongation through ethylene-dependent and independent mechanisms, both hormonal signals converging on AUX1 as a regulatory hub. (nottingham.ac.uk)
- Taken together, our results uncover several regulatory circuits controlling interactions of cytokinin with auxin and ethylene, and support a model in which cytokinin regulates shootward auxin transport to control cell elongation and root growth. (nottingham.ac.uk)
- This review reports the effect of auxins, abscisic acid, cytokinins, gibberellins, and ethylene on microalgal growth and metabolites, as well as the crosstalk of different phytohormones. (mdpi.com)
Synthetic cytokinin3
- It is, therefore, considered a "synthetic cytokinin" with reference to plants. (springer.com)
- CytoFlor is the newest member of the Mycorrhizal Applications/Valent BioSciences plant growth regulator portfolio, which contains 6-benzyladenine (6-BA) a synthetic cytokinin, as the active ingredient. (mycorrhizae.com)
- The plant growth regulator forchlorfenuron, a synthetic cytokinin known to inhibit septin dynamics, likely represents an informative probe for septin function. (edu.au)
Hormones27
- While cytokinin action in vascular plants is described as pleiotropic, this class of plant hormones specifically induces the transition from apical growth to growth via a three-faced apical cell in moss protonema. (wikipedia.org)
- Cytokinins are plant hormones involved in cell division, shoot meristem and leaf formation, chloroplast biogenesis, and senescence. (innovations-report.de)
- Here, we provide a brief overview of the metabolic pathways that regulate the levels of this group of plant hormones and highlight recent advances in the understanding of cytokinin transport. (biologists.org)
- It was recently reported that the human-exclusive pathogen Mycobacterium tuberculosis secretes cytokinins, which had only been known as plant hormones. (asm.org)
- Cytokinins are a major class of plant hormones that are involved in various aspects of plant development, ranging from organ formation and apical dominance to leaf senescence. (biologists.org)
- Cytokinin and auxin have long been known to interact antagonistically, and more recent studies have shown that cytokinins also interact with other plant hormones to regulate plant development. (biologists.org)
- Many aspects of plant development are regulated by antagonistic interactions between the plant hormones auxin and cytokinin, but the molecular mechanisms of this interaction are not understood. (rti.org)
- Koshimizu K, Iwamura H (1986) Cytokinins In Takahashi N (ed) Chemistry of plant hormones. (springer.com)
- Cytokinins, like other plant hormones, affect a diverse array of plant growth and development processes and responses to the environment. (pnas.org)
- Cytokinins (CKs) are pivotal plant hormones that have crucial roles in plant growth and development. (cas.cz)
- Cytokinins are important plant hormones that influence a diverse array of physiological and developmental processes such as root and shoot morphogenesis. (ufl.edu)
- The measurement of the IAA and cytokinin contents showed accumulation of these hormones in the root of experimental plants as compared to the main root of intact plants. (deepdyve.com)
- Also known as 'endophytic' animals, these insects can even manipulate the signaling system in a plant, such as a class of plant hormones called cytokinins, which help plants to grow and to develop seeds and nutrient-storing fruits or young leaves. (elifesciences.org)
- then developed a method to clearly distinguish cytokinins present in the insects from those produced by the plants to test whether T. notatus can transfer these plant hormones during feeding. (elifesciences.org)
- We discovered a feedback loop between the hormones, in which cytokinin activates auxin exporters, while auxin represses cytokinin signalling. (helsinki.fi)
- Cytokinins are essential hormones for plant growth and development and are involved in many physiological functions including the release of lateral buds from apical dominance and the promotion of cell division and differentiation from undifferentiated tissues. (mycorrhizae.com)
- The Nod factor signaling pathway interacts with plant hormones, including cytokinins and gibberellins. (edu.au)
- The second project aimed to study the role of IPTs from the moss Physcomitrella patens in the production of cytokinin hormones. (slu.se)
- Cytokinins are plant hormones that help in growth promoting cell division in plant shoots and roots. (researchnester.com)
- An autoregulatory circuit is identified involving the control of ARR10 and AUX1 expression by cytokinin and auxin, this circuit potentially functioning as an oscillator to integrate the effects of these two hormones. (nottingham.ac.uk)
- While the interaction between different plant hormones such as auxin, cytokinins (CKs), and gibberellins on bud outgrowth (bud release and/or subsequent growth) have been widely studied, recent work has suggested that interactions between SLs and CKs may also be very important [ 3 , 4 ]. (scitechnol.com)
- When a plant is under stress at any given stage of growth, reduced levels of cytokinin growth hormones are produced. (agriorganics.com)
- Plants under stress are unable to produce sufficient cytokinins, the natural plant growth hormones which are necessary for plant growth, nutrient mobilization and distribution, germination, cell division, root development, flowering and seed formation. (agriorganics.com)
- When a plant is under stress at any given stage of growth, it will produce less cytokinin growth hormones. (agriorganics.com)
- The hormones that help plants grow, also known as cytokinins, are also found in coconut water. (medicalnewstoday.com)
- These plant hormones (also cytokinins) are found in higher plants, but also in algae , bacteria and fungi . (wein.plus)
- Cytokinins are a class of plant hormones that promote cell division in plant roots and shoots. (doraagri.com)
Receptor18
- This pathway is initiated by cytokinin binding to a histidine kinase receptor in the endoplasmic reticulum membrane. (wikipedia.org)
- They expressed proteins belonging to the putative receptor proteins (histidine kinase sensors), the candidate histidine phosphotransmitter proteins (AHPs), and the candidate response regulators (ARRs) with and without added cytokinin and measured the expression of a cytokinin-responsive reporter gene. (sciencemag.org)
- The cytokinin pathway appears to involve receptor sensing and to require histidine kinase activity based on the analysis of mutants of the CKI1 and CRE1. (sciencemag.org)
- Dear Colleagues: Will you be so kind to help us with pursuing the research of the cytokinin receptor from maize? (bio.net)
- Previously, we have isolated and characterized a cytokinin-binding protein with the molecular mass of 70 kDa (CBP-70) and showed its receptor properties [1,2]. (bio.net)
- Several antibodies were able to regulate the basic function of the receptor: activation of cytokinin-dependent RNA synthesis [4]. (bio.net)
- We think that elucidation of the correlation between the content of cytokinins in tissue (or in the cell) and the content, synthesis and activity of the receptor is of great scientific and practical interest. (bio.net)
- The Arabidopsis sensor histidine kinase CRE1 was recently reported to be a cytokinin receptor. (sciencemag.org)
- These results suggest that CKI1 is involved in cytokinin signal transduction, possibly as a cytokinin receptor. (sciencemag.org)
- In this study we demonstrate via mathematical modeling that a combination of an inhibitor of the Cytokinin (CK) receptor, Arabidopsis histidine kinase 4 (AHK4) and two morphogens originating from the top cell layer, can plausibly account for the cell lineageindependent centering of WUS expression within SAM. (wur.nl)
- In plants the hormone cytokinin is perceived by members of a small cytokinin receptor family, which are hybrid sensor histidine kinases. (biomedcentral.com)
- Using a live cell hormone-binding assay, we show that cytokinin is bound by a receptor domain predicted to be extracellular, the so called CHASE (cyclases, histidine kinase associated sensory extracellular) domain. (biomedcentral.com)
- The binding of cytokinin to the receptor is thought to cause a conformational change leading to the autophosphorylation of a conserved histidine residue in the cytosolic part of the receptor. (biomedcentral.com)
- Although the CHASE domain is suspected of being the ligand binding domain, no systematic approach has been made with any cytokinin receptor to identify the binding domain unequivocally. (biomedcentral.com)
- The cytokinin receptor AHK4 histidine kinase, identified in Arabidopsis thaliana , presumably acts in concert with downstream components, such as histidine-containing phosphotransfer (HPt) factors (AHPs) and response regulators (ARRs). (oup.com)
- GENTAUR antibody-antibodies.com The Marketplace for Antibodies : CREam of cytokinin signalling: receptor identified. (antibody-antibodies.com)
- CREam of cytokinin signalling: receptor identified. (antibody-antibodies.com)
- The recent identification of CRE1 (a histidine kinase identical to AHK4 and WOL) as the cytokinin receptor of Arabidopsis thaliana is a landmark in cytokinin research. (antibody-antibodies.com)
Phytohormones7
- Cytokinins (CK) are a class of plant growth substances (phytohormones) that promote cell division, or cytokinesis, in plant roots and shoots. (wikipedia.org)
- In plants, the cytokinin (CK) phytohormones regulate numerous biological processes, including responses to environmental stresses, via a complex network of CK signaling. (nih.gov)
- Cytokinins are a class of phytohormones involved in various physiological events of plants. (sciencemag.org)
- Although interactions of cytokinins with salicylic acid and auxin have been indicated, the complete network of cytokinin interactions with other immunity-relevant phytohormones is not yet understood. (apsnet.org)
- The formation of LR primordia is regulated antagonistically by the phytohormones cytokinin and auxin. (cpib.ac.uk)
- Cytokinins are phytohormones essential for cytokinesis and many other physiological and developmental processes in planta. (usda.gov)
- Among the recognized signaling molecules, a class of phytohormones known as the cytokinins functions as both local and long-distance regulatory signals for the coordination of plant development. (usda.gov)
Gene16
- The impact of pathogen-derived cytokinins on the total cytokinin content of infected tissue and host gene expression was minimal in wild type plants. (springer.com)
- It is an object of the present invention to clarify an enzyme gene capable of directly regulating the amount of active cytokinins synthesized and to provide a transformed plant with a regulated amount of cytokinins using the aforementioned gene. (google.com)
- d) a gene encoding a protein which consists of an amino acid sequence derived from the amino acid sequence as shown in any one of SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14 and 16 by deletion, substitution or addition of one or several amino acid residues and has activity of catalyzing reactions that synthesize active cytokinins from nucleotide cytokinins. (google.com)
- Hwang and Sheen established an assay that involved transfection of mesophyll protoplasts of Arabidopsis leaves to monitor the activation of a cytokinin response gene. (sciencemag.org)
- Expression of AHP1 and AHP2 activated the reporter gene, and these two proteins exhibited cytokinin-dependent nuclear translocation. (sciencemag.org)
- Expression of the A-type ARR proteins resulted in inhibition of the cytokinin-stimulated reporter gene activity, and expression of A-type ARR proteins activated the reporter gene even in the absence of added cytokinin (ARR2 being the strongest activator). (sciencemag.org)
- We used a steroid-inducible system to show that the transcription factor-type response regulator ARR1 directs transcriptional activation of the ARR6 gene, which responds to cytokinins without de novo protein synthesis. (sciencemag.org)
- Here, we show cytokinins induced the strong expression of the M. tuberculosis gene Rv0077c. (asm.org)
- A gene, CKI1 , which was tagged in four of the five mutants and induced typical cytokinin responses after introduction and overexpression in plants, was cloned. (sciencemag.org)
- Finally, enzymes encoded by the LOG gene family catalyse the conversion of tzRMP into an active cytokinin form, in this case tz. (biologists.org)
- In addition, the LONELY GUY (LOG) gene family encodes enzymes that convert cytokinin from an inactive to an active form. (biologists.org)
- However, cytokinin failed to inhibit auxin-induced LeSAUR gene expression, an auxin response that is affected by the dgt mutation. (rti.org)
- An integrated set of approaches was used to define the targets of the type-B response regulators, a key set of transcription factors that control cytokinin-dependent gene expression. (pnas.org)
- In the screen for upstream regulators we identified a LONG PALE HYPOCOTYL (LPH) gene whose activity is indispensable for spatiotemporally correct expression of CYTOKININ INDEPENDENT-1 (CKI1), encoding the constitutively active sensor histidine kinase that activates MSP signaling. (muni.cz)
- Because the phytohormone cytokinins play an essential role in flower development, particularly pistil development, in this study, we elevated the cytokinin levels in flowers through transgenic expression of a cytokinin biosynthetic gene () from Arabidopsis under the control of a orthologue of () promoter that is predominantly active in flowers. (physiciansweekly.com)
- the AHP6 gene inhibits cytokinin signalling at the protoxylem position during normal vascular development. (helsinki.fi)
Abscisic acid2
- Based on these results, we conclude that reduction of abscisic acid levels by enhanced abscisic acid catabolism strongly contributes to cytokinin-mediated resistance effects. (apsnet.org)
- Thus, the identified cytokinin-abscisic acid antagonism is a novel regulatory mechanism in plant immunity. (apsnet.org)
Regulate3
- It was hypothesized that cytokinin may affect enzymes that regulate protein synthesis and degradation. (wikipedia.org)
- Because of their ability to activate transcription and regulate protein activity, RRs have been proposed to coordinate most of physiological processes regulated by cytokinin. (ufl.edu)
- Natural cytokinins such as those found in Nitrozyme will help control and regulate: germination - root development - nutrition uptake - plant tissue composition - tillering - flowering -seed and fruit set. (agriorganics.com)
Biosynthetic3
- Chen CM, Ertl JR, Leisner SM, Chang CC (1985) Localization of cytokinin biosynthetic sites in pea plants and carrot roots. (springer.com)
- An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins. (diva-portal.org)
- An improved method for determining the relative biosynthetic rate of isoprenoid cytokinins has been developed. (diva-portal.org)
Pathways4
- Unlike other two-component pathways that converge on mitogen-activated protein kinase pathways (see Santos and Shiozaki ), cytokinin signaling stimulates the nuclear translocation of a histidine phosphotransmitter protein (AHP), which leads to the activation of the response regulators, which are themselves transcription factors. (sciencemag.org)
- However, the interaction between the cytokinin and gibberellin signaling pathways is not fully understood. (edu.au)
- EN] The ability of plants to provide a plastic response to environmental cues relies on the connectivity between signaling pathways. (upv.es)
- Pleiotropic effects have made studies of this hormone difficult, and cytokinin signalling pathways have long remained elusive. (antibody-antibodies.com)
Tissues9
- Cambium and other actively dividing tissues also synthesize cytokinins. (wikipedia.org)
- Cytokinins have been shown to slow aging of plant organs by preventing protein breakdown, activating protein synthesis, and assembling nutrients from nearby tissues. (wikipedia.org)
- Also, I would be interested to hear any comments concerning the use of antibodies to locate cytokinins in plant tissues. (bio.net)
- Different substrate specificities, and the pH profiles of cytokinin-degrading enzymes extracted from different barley tissues, are also presented. (sigmaaldrich.com)
- Parallel determination of auxin and cytokinin levels within plant organs and tissues represents an invaluable tool for studies of their physiological effects and mutual interactions. (cas.cz)
- Here we describe a method that is based on single step purification protocol followed by LC-MS separation and detection for parallel analysis of auxins, cytokinins and their metabolites in various plant tissues and cell cultures. (cas.cz)
- Endophytic insects provide the textbook examples of herbivores that manipulate their host plant's physiology, putatively altering source/sink relationships by transferring cytokinins (CK) to create 'green islands' that increase the nutritional value of infested tissues. (elifesciences.org)
- While cytokinin is frequently thought to form gradients guiding developmental processes in the Arabidopsis shoot and root, we showed that an informative cytokinin gradient cannot form on the scale of these tissues via diffusion. (helsinki.fi)
- Cytokinin synthesis takes place primarily in the meristematic tissues. (brainkart.com)
Isoprenoid2
- THe proposed FAB method was used to identify and quantify 10 isoprenoid cytokinins in Arabidopsis thaliana, including free bases, nucleosides, nucleotides and glucosides. (nih.gov)
- The reduction rate was high for isoprenoid cytokinins that showed formation of a charge-transfer complex of reduced enzyme with bound cytokinin imine. (portlandpress.com)
Responses10
- The type-B Arabidopsis response regulators (ARRs) are DNA-binding transcriptional activators that are required for cytokinin responses. (nih.gov)
- Analysis of cytokinin precursors, active components and conjugates by microanalytical techniques was consistent with these transcriptional responses. (springer.com)
- The phytohormone cytokinin plays diverse roles in plant development, influencing many agriculturally important processes, including growth, nutrient responses and the response to biotic and abiotic stresses. (biologists.org)
- These cytokinin responses in Arabidopsis are at least partly triggered through the recognition of cytokinins by the sensor protein CRE1, which is a member of the protein histidine kinase family ( 3 , 4 ). (sciencemag.org)
- Five Arabidopsis thaliana mutants that exhibit typical cytokinin responses, including rapid cell division and shoot formation in tissue culture in the absence of exogenous cytokinin, were isolated by activation transferred DNA tagging. (sciencemag.org)
- arr1 buds have wild-type responses to auxin and cytokinin but are resistant to strigolactone. (plantphysiol.org)
- Results shed light on the physiological role of the type-B ARRs in regulating the cytokinin response, mechanism of type-B ARR activation, and basis by which cytokinin regulates diverse aspects of growth and development as well as responses to biotic and abiotic factors. (pnas.org)
- The plant hormone cytokinin affects a diverse array of growth and development processes and responses to the environment. (pnas.org)
- Scalp Induction Rate Responses to Cytokinins on Proliferating Shoot-Tips of Banana Cultivars (Musa spp. (thescipub.com)
- The auxin importer AUX1 functions as a positive regulator of cytokinin responses in the root, AUX1 mutants specifically affecting the ability of cytokinin to inhibit cell elongation but not cell proliferation. (nottingham.ac.uk)
Role of cytokinin3
- I confirmed the negative role of cytokinin action in root developmental processes previously hypothesized in other plant systems, and obtained direct evidence that links a specific type RR (PtRR13) with inhibition of adventitious root formation. (ufl.edu)
- Adventitious shoot regeneration from vegetative shoot apices in pear and putative role of cytokinin accumulation in the morphogenetic process. (usda.gov)
- Supplement of cytokinin restores the phenotypes of rax ( Regulator of Axillary Meristems ) mutant which displays a reduced number of axillary meristems due to the initiation defects [ 12 ], implying a predominant role of cytokinin during axillary meristem formation. (biomedcentral.com)
Auxins and Cytokinins2
Analysis of Cytokinins1
- For example, the use of HPLC for the analysis of cytokinins and their derivatives is superior to the classical technique of gas chromatography, since the liquid mobile phase allows the separation and recovery of cytokinins and their derivatives which are not readily volatilized. (springer.com)
Signaling10
- Cytokinin moves from the roots into the shoots, eventually signaling lateral bud growth. (wikipedia.org)
- Cytokinin signaling in plants is mediated by a two-component phosphorelay. (wikipedia.org)
- However, only in plants and the protist Dictyostelium have cytokinins been definitively shown to act in cellular signaling. (biologists.org)
- Collectively, these findings show cytokinins signal transcriptional changes that can affect M. tuberculosis acid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae. (asm.org)
- However, the molecular mechanism underlying the interaction between light and cytokinin signaling remains elusive. (muni.cz)
- Changes in CKI1 expression observed in lph/hy1-7 and phy mutants correlate with misregulation of MSP signaling, changed cytokinin sensitivity and developmental aberrations, previously shown to be associated with cytokinin and/or CKI1 action. (muni.cz)
- A better understanding of the molecular mechanisms by which cytokinin signaling is connected to specific traits can provide the genetic tools to improve agronomic characteristics (e.g, stem wood production per unit root biomass). (ufl.edu)
- Cytokinin signaling resembles two-component systems from bacteria and yeast in which an external signal is sensed by a histidine kinase (HK) and then transferred to a response regulator (RR). (ufl.edu)
- Activation of cytokinin signaling through the homeodomain-containing transcription factors KNOX is essential for nodule formation. (edu.au)
- In summary, we show that the inhibition of cytokinin signaling through AHP6 is required to establish the correct pattern during LR initiation. (cpib.ac.uk)
Transcriptional1
- Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. (asm.org)
Endogenous cytokinins1
- ALTHOUGH the occurrence of endogenous cytokinins has been studied extensively in higher plants, reports for the algae are restricted to marine planktonic forms 1 . (nature.com)
Regulates1
- The mutual inhibition between auxin and cytokinin regulates the extent of their domains during vascular patterning. (helsinki.fi)
Enzymes1
- To name a few - Cytokinins - Auxins - Enzymes - Giberellins - Ethylenes - plus many Micro-nutrients. (agriorganics.com)
Mutants4
- Intriguingly, plasmodial development was slowed and spore viability reduced in these mutants indicating a potential role for cytokinins in plasmodial development. (springer.com)
- Since root colonization is not affected in the cytokinin mutants, we propose that cytokinins are required for P. indica --induced growth promotion. (apsnet.org)
- The cytokinin module ARABIDOPSIS HISTIDINE KINASE3 (AHK3)/ARABIDOPSIS RESPONSE REGULATOR1 (ARR1)/ARR12 was found to interact with the GR24-dependent reduction in LR development, because mutants in this pathway rendered LR development insensitive to GR24. (ugent.be)
- We used a combination of mutants and pharmacological treatments to investigate how mutually exclusive auxin and cytokinin signalling domains are maintained in the Arabidopsis stele. (helsinki.fi)
Signal transduction in plant1
- Our research is open for cooperation and joint studies of the mechanisms of cytokinin reception and cytokinin signal transduction in plant. (bio.net)
Receptors6
- We discuss the perception of cytokinin by the ER-localized histidine kinase receptors, the role of the AHPs in mediating the transfer of the phosphoryl group from the receptors to the response regulators (ARRs), and finally the role of the large ARR family in cytokinin function. (nih.gov)
- We offer substituted 6-(alkylbenzylamino)-purin derivatives as cytokinin re-ceptor antagonists to provide cytokinin analogons for growth regulation in plants and to offer the possibility for a specific selectivity for cytokinin receptors without being toxic for animal cells. (innovations-report.de)
- Cytokinins are perceived by membrane-localized histidine-kinase receptors and are transduced through a His-Asp phosphorelay to activate a family of transcription factors in the nucleus. (biologists.org)
- The CHASE domain occurs not only in plant cytokinin receptors but also in numerous orphan receptors in lower eukaryotes and bacteria. (biomedcentral.com)
- Taking advantage of this fact, we used an evolutionary proteomics approach to identify amino acids important for cytokinin binding by looking for residues conserved in cytokinin receptors, but not in other receptors. (biomedcentral.com)
- Among higher eukaryotes the domain is found only in plants as part of specific sensor histidine kinases, the cytokinin receptors. (biomedcentral.com)
Regulators2
- Of the commercially available plant growth regulators, cytokinins is one of the most important groups. (mycorrhizae.com)
- Keywords: Arabidopsis - Cytokinin - Histidine kinase - Phosphorelay -Response regulators - Signal transduction. (oup.com)
CRE14
- Cytokinins are perceived by three histidine kinases--CRE1/WOL/AHK4, AHK2, and AHK3--which initiate intracellular phosphotransfer. (nih.gov)
- The CKI1 sensor is constitutively active in this system, but the CRE1 sensor requires added cytokinins for activity. (sciencemag.org)
- This fact, together with characteristics of ARR1 -overexpressing plants and arr1 mutant plants, indicates that the phosphorelay to ARR1, probably from CRE1, constitutes an intracellular signal transduction occurring immediately after cytokinin perception. (sciencemag.org)
- Mutations have been identified in CRE1, and the phenotype of loss-of-function mutations sheds new light on the role of cytokinins in plant development. (antibody-antibodies.com)
Effect of cytokinin3
- The ratio of auxin to cytokinin plays an important role in the effect of cytokinin on plant growth. (wikipedia.org)
- This bud induction can be pinpointed to differentiation of a specific single cell, and thus is a very specific effect of cytokinin. (wikipedia.org)
- This study aimed to evaluate the effect of cytokinin and gibberelin on the agronomic and physicochemical characteristics of banana fruits cv. (scielo.br)
Gibberellin1
- Cytokinin and gibberellin, alone or associated, regardless of formation period and position, did not affect the size and physicochemical characteristics of fruits, only delayed the bunch harvest. (scielo.br)
Synthesis3
- Catalyzes the hydrolytic removal of ribose 5'-monophosphate from nitrogen N6-modified adenosines, the final step of bioactive cytokinin synthesis. (uniprot.org)
- Is involved in the synthesis of isopentenyladenine (iP) and 2-methylthio-iP (2MeS-iP), the most abundant cytokinins detected in M.tuberculosis lysates and supernatants. (uniprot.org)
- Plant tRNA isopentenyltransferases (tRNA-IPT, EC 2.5.1.8) have two roles, modification of tRNA and contribution to cytokinin synthesis. (slu.se)
Degradation1
- tRNAs with anticodons that start with a uridine and carrying an already-prenylated adenosine adjacent to the anticodon release on degradation the adenosine as a cytokinin. (wikipedia.org)
Buds3
- More cytokinin induces growth of shoot buds, while more auxin induces root formation. (wikipedia.org)
- Decapitation and pharmacological experiments on dormant buds showed that the reduction of auxin accumulation triggers the regeneration of vegetative shoots in dormant buds, and exogenous cytokinin application triggers cell fate turnover and generation of reproductive shoots. (biomedcentral.com)
- Cytokinins enhance plant growth by stimulating cell division and increase the sprouting of lateral buds. (brainkart.com)
Lateral2
- Finally, we discovered a potential link between the auxin-cytokinin feedback loop in the root tip and the initiation of lateral roots. (helsinki.fi)
- AUX1 is required for cytokinin-dependent changes of auxin activity in the lateral root cap associated with the control of cell elongation. (nottingham.ac.uk)
Inhibit1
- In Arabidopsis, cytokinins inhibit root growth through effects on cell proliferation and cell elongation. (nottingham.ac.uk)
Histidine kinases1
- Upon binding of cytokinin, the two histidine kinases phosphorylate their histidine residues reciprocally ( autophosphorylation ). (brainkart.com)
Levels of cytokinins3
- Certain plant-associated prokaryotes such as Corynebacteriurn fascians and Agrobacterium tumefaciens are known to produce substantial levels of cytokinins. (oregonstate.edu)
- In view of the fragmentary and presumptive evidence available on the quantitative and qualitative levels of cytokinins produced by A. tumefaciens, it was decided to conduct a study of the cytokinins produced by A. tumefaciens. (oregonstate.edu)
- The fungus produces relatively high levels of cytokinins, and the cytokinin levels are higher in colonized roots compared with the uncolonized controls. (apsnet.org)
Molecular3
- M. Febs Letters (423)2 (1998) pp. 239-242 Thank you in advance, Sincerely yours, Feodor A. Brovko, Head of research team, Ph.D.(Biology), Senior researcher P.S. My team studies the molecular mechanisms of cytokinin signal reception in plant using biochemical and immunochemical methods, and methods of genetic engineering. (bio.net)
- However, the molecular players involved in cytokinin repression are still unknown. (cpib.ac.uk)
- Molecular and Biochemical Characterization of a Cytokinin Oxid. (mysciencework.com)
Senescence6
- Our previous successful attempts to delay premature senescence and growth inhibition under abiotic stress conditions by autoregulation of cytokinins (CKs) levels constitute a generic technology toward the development of highly productive plants. (frontiersin.org)
- Cytokinins impact the apical dominance, leaf senescence, and axillary bud growth as well in plants. (researchnester.com)
- On the back of multiplying cytokinins uses in growing end-use industries for applications such as breaking of seed dormancy, counteraction of apical dominance, cell division and delay in senescence, Asia Pacific is budding as a regional Cytokinins and its derivatives consumption market. (researchnester.com)
- They excrete cytokinin with their saliva and thus prevent senescence of the leaves on which they are feeding. (brainkart.com)
- Transgenic tobacco plants in which the activity of cytokinin syn-thase in the leaves is increased have a much longer lifespan than normal plants, since their senescence is suppressed by the enhanced production of cytokinin. (brainkart.com)
- Cytokinin promotes cell division (cytokinesis), cell growth, differentiation and affect apical dominance, axillary bud growth, leaf senescence and so on. (aribidopsis.com)
Roots4
- Synthesized in roots, cytokinins move upward in the xylem (woody tissue) and pass into the leaves and fruits, where they are required for normal growth, for cell differentiation, and, in conjunction with auxin (another plant hormone), to promote cell division. (britannica.com)
- These will be used for immunolocalization of cytokinins in roots. (bio.net)
- In a similar manner to protoxylem formation in Arabidopsis roots, in which AHP6 (ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6) acts as a cytokinin inhibitor, we reveal that AHP6 also functions as a cytokinin repressor during early stages of LR development. (cpib.ac.uk)
- Cytokinins are synthesized in the roots, they will move upward and enter the leaves and fruits of the plants, to maintain normal growth and cell differentiation of them. (doraagri.com)
Total cytokinin1
- Deineko, E. 2009-11-06 00:00:00 A comparative analysis of IAA and total cytokinin contents at various developmental stages of floral morphogenesis (I-XII) in transgenic tobacco (Nicotiana tabacum L.) plants with mutant phenotypes was performed. (deepdyve.com)
Interactions1
- Altogether, my thesis reveals a key role for mutually inhibitory auxin-cytokinin interactions in vascular development and links these findings to other developmental contexts. (helsinki.fi)
Xylem2
- Typically, cytokinins are transported in the xylem. (wikipedia.org)
- This process is mediated by the non-cell-autonomous repression of cytokinin signalling in the root meristem, and leads to distinct phloem- and xylem-pole-associated endodermal cells. (wur.nl)
Induce4
- For example, cytokinins have been described to induce resistance against Pseudomonas syringae in Arabidopsis thaliana and Nicotiana tabacum. (wikipedia.org)
- Cytokinins induce a variety of physiological events, including cell division, chloroplast development, and shoot formation ( 1 , 2 ). (sciencemag.org)
- Only a two to three week culture period on the high cytokinin medium was necessary to induce adventitious bud formation. (oregonstate.edu)
- In nature some plant associated bacteria and fungi produce auxin and cytokinin to induce unlimited cell division, which results in tumor growth of the plant. (brainkart.com)
Antibodies1
- Does anyone have information regarding acquisition of antibodies against cytokinins? (bio.net)
ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN1
- Here we show that another type of hormone-based inhibitory fields, generated directly downstream of auxin by intercellular movement of the cytokinin signalling inhibitor ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6 (AHP6), is involved in regulating phyllotactic patterns. (cpib.ac.uk)
Mechanism5
- Cytokinins participate in local and long-distance signalling, with the same transport mechanism as purines and nucleosides. (wikipedia.org)
- Nevertheless, our findings highlight the need for a cytokinin patterning mechanism, such as directed cytokinin transport or patterning of the cytokinin perception machinery, since diffusion cannot form the observed cytokinin patterns. (helsinki.fi)
- The mechanism of cytokinin-induced cell expansion in cotyledons excised from dark-grown seedlings of radish (Raphanus sativus L.) and cucumber (Cucumus sativus L.) was studied. (semanticscholar.org)
- Here, we uncover a mechanism by which auxin and cytokinin coordinate the dormancy and outgrowth of axillary bud in strawberry runner. (biomedcentral.com)
- The specific illustration presented, CRK10 , implies a new mechanism for the perception of cytokinin, a classic plant hormone. (biomedcentral.com)
Crosstalk1
- The crosstalk between cytokinin response and light is known for a long time. (muni.cz)
Type of cytokinin1
- The experiments revealed that the insects' bodies contained large quantities of a type of cytokinin, even when insects were raised on artificial diets. (elifesciences.org)
Shown that cytokinin2
- At the Biodynamics Research Team in the Plant Productivity Systems Research Group at the RIKEN Plant Science Center, investigations have shown that cytokinin, a plant hormone that activates cell division, is closely associated with rice yields. (innovations-report.com)
- It has previously been shown that cytokinin has an inhibitory effect on the patterning events occurring during LR formation. (cpib.ac.uk)
Effects of cytokinin1
- To test whether cytokinin controls plant development through inhibiting an early step in the auxin response pathway, we compared the effects of cytokinin with those of the dgt (diageotropica) mutation, which is known to block rapid auxin reactions of tomato (Lycopersicon esculentum) hypocotyls. (rti.org)
Content of cytokinins1
- In plants with the phenotype "wavy corolla", total content of cytokinins was lower than in control at all developmental stages (except for stage I), whereas their dynamics coincided with that in control flowers. (deepdyve.com)