An enzyme that catalyzes the biosynthesis of cysteine in microorganisms and plants from O-acetyl-L-serine and hydrogen sulfide. This enzyme was formerly listed as EC 4.2.99.8.
An enzyme that catalyzes the conversion of L-SERINE to COENZYME A and O-acetyl-L-serine, using ACETYL-COA as a donor.
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
A species of gram-negative, aerobic bacteria that is a fast-growing and soybean-nodulating innoculant.
A class of enzymes that catalyze the cleavage of C-C, C-O, and C-N, and other bonds by other means than by hydrolysis or oxidation. (Enzyme Nomenclature, 1992) EC 4.
A widely cultivated plant, native to Asia, having succulent, edible leaves eaten as a vegetable. (From American Heritage Dictionary, 1982)
An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.
Enzymes that catalyze the cleavage of a carbon-oxygen bond by means other than hydrolysis or oxidation. EC 4.2.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
This is the active form of VITAMIN B 6 serving as a coenzyme for synthesis of amino acids, neurotransmitters (serotonin, norepinephrine), sphingolipids, aminolevulinic acid. During transamination of amino acids, pyridoxal phosphate is transiently converted into pyridoxamine phosphate (PYRIDOXAMINE).
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Plant cell inclusion bodies that contain the photosynthetic pigment CHLOROPHYLL, which is associated with the membrane of THYLAKOIDS. Chloroplasts occur in cells of leaves and young stems of plants. They are also found in some forms of PHYTOPLANKTON such as HAPTOPHYTA; DINOFLAGELLATES; DIATOMS; and CRYPTOPHYTA.
ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The rate dynamics in chemical or physical systems.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.
A subclass of peptide hydrolases that depend on a CYSTEINE residue for their activity.
Enzyme that catalyzes the first step of the tricarboxylic acid cycle (CITRIC ACID CYCLE). It catalyzes the reaction of oxaloacetate and acetyl CoA to form citrate and coenzyme A. This enzyme was formerly listed as EC 4.1.3.7.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Arrest of cell locomotion or cell division when two cells come into contact.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
A phenol obtained from thyme oil or other volatile oils used as a stabilizer in pharmaceutical preparations, and as an antiseptic (antibacterial or antifungal) agent. It was formerly used as a vermifuge.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.
Compounds with a core of 10 carbons generally formed via the mevalonate pathway from the combination of 3,3-dimethylallyl pyrophosphate and isopentenyl pyrophosphate. They are cyclized and oxidized in a variety of ways. Due to the low molecular weight many of them exist in the form of essential oils (OILS, VOLATILE).
Oils which evaporate readily. The volatile oils occur in aromatic plants, to which they give odor and other characteristics. Most volatile oils consist of a mixture of two or more TERPENES or of a mixture of an eleoptene (the more volatile constituent of a volatile oil) with a stearopten (the more solid constituent). The synonym essential oils refers to the essence of a plant, as its perfume or scent, and not to its indispensability.
The mechanical process of cooling.
A genus of bacteria which may be found in the feces of animals and man, on vegetation, and in silage. Its species are parasitic on cold-blooded and warm-blooded animals, including man.
The apparent tendency of certain diseases to appear at earlier AGE OF ONSET and with increasing severity in successive generations. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.
Exposure of the male parent, human or animal, to potentially harmful chemical, physical, or biological agents in the environment or to environmental factors that may include ionizing radiation, pathogenic organisms, or toxic chemicals that may affect offspring.
A species of nematode that is widely used in biological, biochemical, and genetic studies.
A flammable, poisonous gas with a characteristic odor of rotten eggs. It is used in the manufacture of chemicals, in metallurgy, and as an analytical reagent. (From Merck Index, 11th ed)
Proteins from the nematode species CAENORHABDITIS ELEGANS. The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS.
Fatty acid biopolymers that are biosynthesized by microbial polyhydroxyalkanoate synthase enzymes. They are being investigated for use as biodegradable polyesters.
A gram-negative, facultatively chemoautotrophic bacterium, formerly called Wautersia eutropha, found in water and soil.
Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.
A species of gram-negative bacteria in the genus PSEUDOMONAS. It is isolated from oil-water emulsions used as lubricants and cooling agents in the cutting and grinding of materials.
Enzymes from the transferase class that catalyze the transfer of acyl groups from donor to acceptor, forming either esters or amides. (From Enzyme Nomenclature 1992) EC 2.3.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
Impaired ability in numerical concepts. These inabilities arise as a result of primary neurological lesion, are syndromic (e.g., GERSTMANN SYNDROME ) or acquired due to brain damage.
Educational institutions.
Branch of medicine concerned with the prevention and control of disease and disability, and the promotion of physical and mental health of the population on the international, national, state, or municipal level.
An activating KIR receptor that contains D0, D1, and D2 extracellular immunoglobulin-like domains and a short cytoplasmic tail.
The activities and endeavors of the public health services in a community on any level.
Management of public health organizations or agencies.

Purification and characterization of serine acetyltransferase from Escherichia coli partially truncated at the C-terminal region. (1/159)

Incubation of serine acetyltransferase (SAT) from Escherichia coli at 25 degrees C in the absence of protease inhibitors yielded a truncated SAT. The truncated SAT was much less sensitive to feedback inhibition than the wild-type SAT. Analyses of the N- and C-terminal amino acid sequences found that the truncated SAT designated as SAT delta C20 was a resultant form of the wild-type SAT cleaved between Ser 253 and Met 254, deleting 20 amino acid residues from the C-terminus. Based on these findings, we constructed a plasmid containing an altered cysE gene encoding the truncated SAT. SAT delta C20 was produced using the cells of E. coli JM70 transformed with the plasmid and purified to be homogeneous on an SDS-polyacrylamide gel. Properties of the purified SAT delta C20 were investigated in comparison with those of the wild-type SAT and Met-256-Ile mutant SAT, which was isolated by Denk and Bock but not purified (J. Gen. Microbiol., 133, 515-525 (1987)). SAT delta C20 was composed of four identical subunits like the wild-type SAT and Met-256-Ile mutant SAT. Specific activity, optimum pH for reaction, thermal stability, and stability to reagents for SAT delta C20 were similar those for the wild-type SAT and Met-256-Ile mutant SAT. However, SAT delta C20 did not form a complex with O-acetylserine sulfhydrylase-A (OASS-A), a counterpart of the cysteine synthetase and did not reduce OASS activity in contrast to the wild-type SAT and Met-256-Ile mutant SAT.  (+info)

Pathways of assimilative sulfur metabolism in Pseudomonas putida. (2/159)

Cysteine and methionine biosynthesis was studied in Pseudomonas putida S-313 and Pseudomonas aeruginosa PAO1. Both these organisms used direct sulfhydrylation of O-succinylhomoserine for the synthesis of methionine but also contained substantial levels of O-acetylserine sulfhydrylase (cysteine synthase) activity. The enzymes of the transsulfuration pathway (cystathionine gamma-synthase and cystathionine beta-lyase) were expressed at low levels in both pseudomonads but were strongly upregulated during growth with cysteine as the sole sulfur source. In P. aeruginosa, the reverse transsulfuration pathway between homocysteine and cysteine, with cystathionine as the intermediate, allows P. aeruginosa to grow rapidly with methionine as the sole sulfur source. P. putida S-313 also grew well with methionine as the sulfur source, but no cystathionine gamma-lyase, the key enzyme of the reverse transsulfuration pathway, was found in this species. In the absence of the reverse transsulfuration pathway, P. putida desulfurized methionine by the conversion of methionine to methanethiol, catalyzed by methionine gamma-lyase, which was upregulated under these conditions. A transposon mutant of P. putida that was defective in the alkanesulfonatase locus (ssuD) was unable to grow with either methanesulfonate or methionine as the sulfur source. We therefore propose that in P. putida methionine is converted to methanethiol and then oxidized to methanesulfonate. The sulfonate is then desulfonated by alkanesulfonatase to release sulfite for reassimilation into cysteine.  (+info)

Cysteine biosynthesis in Chlamydomonas reinhardtii. Molecular cloning and regulation of O-acetylserine(thiol)lyase. (3/159)

A cDNA, Cys1ACr, encoding an isoform of O-acetylserine(thiol) lyase has been isolated from Chlamydomonas reinhardtii, using a PCR-based approach. The inclusion of dimethylsulfoxide in the PCR reaction has been demonstrated to be essential for the correct amplification of C. reinhardtii templates with complex secondary structures caused by a high G + C content. The deduced amino acid sequence exhibited highest similarity with plant O-acetylserine(thiol)lyase isoforms, indicating that the C. reinhardtii enzyme was structurally more similar to higher plant O-acetylserine(thiol)lyase than to the corresponding prokaryotic enzymes. The N-terminal extension present in Cys1ACr showed several characteristics of an organellar transit peptide, with a length typical for C. reinhardtii. Southern blot analysis suggested that the C. reinhardtii genome may contain a single copy of the organellar O-acetylserine(thiol)lyase gene. O-acetylserine(thiol)lyase activity was strongly induced by sulfur-deficient conditions (up to sevenfold the level observed in a sulfur-repleted cell culture) and required the presence of a nitrogen source. Northern blot analysis showed a different pattern of regulation of Cys1ACr to that observed at the activity level. To obtain an increase of transcript abundance a longer period of sulfur limitation was required, reaching a maximum level of approximately threefold Cys1ACr mRNA when compared with the level of a sulfate-grown culture.  (+info)

O-Acetylserine sulfhydrylase from Methanosarcina thermophila. (4/159)

Cysteine is the major source of fixed sulfur for the synthesis of sulfur-containing compounds in organisms of the Bacteria and Eucarya domains. Though pathways for cysteine biosynthesis have been established for both of these domains, it is unknown how the Archaea fix sulfur or synthesize cysteine. None of the four archaeal genomes sequenced to date contain open reading frames with identities to either O-acetyl-L-serine sulfhydrylase (OASS) or homocysteine synthase, the only sulfur-fixing enzymes known in nature. We report the purification and characterization of OASS from acetate-grown Methanosarcina thermophila, a moderately thermophilic methanoarchaeon. The purified OASS contained pyridoxal 5'-phosphate and catalyzed the formation of L-cysteine and acetate from O-acetyl-L-serine and sulfide. The N-terminal amino acid sequence has high sequence similarity with other known OASS enzymes from the Eucarya and Bacteria domains. The purified OASS had a specific activity of 129 micromol of cysteine/min/mg, with a K(m) of 500 +/- 80 microM for sulfide, and exhibited positive cooperativity and substrate inhibition with O-acetyl-L-serine. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a single band at 36 kDa, and native gel filtration chromatography indicated a molecular mass of 93 kDa, suggesting that the purified OASS is either a homodimer or a homotrimer. The optimum temperature for activity was between 40 and 60 degrees C, consistent with the optimum growth temperature for M. thermophila. The results of this study provide the first evidence for a sulfur-fixing enzyme in the Archaea domain. The results also provide the first biochemical evidence for an enzyme with the potential for involvement in cysteine biosynthesis in the Archaea.  (+info)

Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes? (5/159)

The pathway of cysteine biosynthesis in archaea is still unexplored. Complementation of a cysteine auxotrophic Escherichia coli strain NK3 led to the isolation of the Methanosarcina barkeri cysK gene [encoding O-acetylserine (thiol)-lyase-A], which displays great similarity to bacterial cysK genes. Adjacent to cysK is an open reading frame orthologous to bacterial cysE (serine transacetylase) genes. These two genes could account for cysteine biosynthesis in this archaeon. Analysis of recent genome data revealed the presence of bacteria-like cysM genes [encoding O-acetylserine (thiol)-lyase-B] in Pyrococcus spp., Sulfolobus solfataricus, and Thermoplasma acidophilum. However, no orthologs for these genes can be found in Methanococcus jannaschii, Methanobacterium thermoautotrophicum, and Archaeoglobus fulgidus, implying the existence of unrecognizable genes for the same function or a different cysteine biosynthesis pathway.  (+info)

Regulation of sulfate assimilation by nitrogen in Arabidopsis. (6/159)

Using Arabidopsis, we analyzed the effect of omission of a nitrogen source and of the addition of different nitrogen-containing compounds on the extractable activity and the enzyme and mRNA accumulation of adenosine 5'-phosphosulfate reductase (APR). During 72 h without a nitrogen source, the APR activity decreased to 70% and 50% of controls in leaves and roots, respectively, while cysteine (Cys) and glutathione contents were not affected. Northern and western analysis revealed that the decrease of APR activity was correlated with decreased mRNA and enzyme levels. The reduced APR activity in roots could be fully restored within 24 h by the addition of 4 mM each of NO(3)(-), NH(4)(+), or glutamine (Gln), or 1 mM O-acetylserine (OAS). (35)SO(4)(2-) feeding showed that after addition of NH(4)(+), Gln, or OAS to nitrogen-starved plants, incorporation of (35)S into proteins significantly increased in roots; however, glutathione and Cys labeling was higher only with Gln and OAS or with OAS alone, respectively. OAS strongly increased mRNA levels of all three APR isoforms in roots and also those of sulfite reductase, Cys synthase, and serine acetyltransferase. Our data demonstrate that sulfate reduction is regulated by nitrogen nutrition at the transcriptional level and that OAS plays a major role in this regulation.  (+info)

beta-Cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis. (7/159)

beta-Cyano-alanine synthase (CAS; EC 4.4.1.9) plays an important role in cyanide metabolism in plants. Although the enzymatic activity of beta-cyano-Ala synthase has been detected in a variety of plants, no cDNA or gene has been identified so far. We hypothesized that the mitochondrial cysteine synthase (CS; EC 4.2.99. 8) isoform, Bsas3, could actually be identical to CAS in spinach (Spinacia oleracea) and Arabidopsis. An Arabidopsis expressed sequence tag database was searched for putative Bsas3 homologs and four new CS-like isoforms, ARAth;Bsas1;1, ARAth;Bsas3;1, ARAth;Bsas4;1, and ARAth;Bsas4;2, were identified in the process. ARAth;Bsas3;1 protein was homologous to the mitochondrial SPIol;Bsas3;1 isoform from spinach, whereas ARAth;Bsas4;1 and ARAth;Bsas4;2 proteins defined a new class within the CS-like proteins family. In contrast to spinach SPIol;Bsas1;1 and SPIol;Bsas2;1 recombinant proteins, spinach SPIol;Bsas3;1 and Arabidopsis ARAth;Bsas3;1 recombinant proteins exhibited preferred substrate specificities for the CAS reaction rather than for the CS reaction, which identified these Bsas3 isoforms as CAS. Immunoblot studies supported this conclusion. This is the first report of the identification of CAS synthase-encoding cDNAs in a living organism. A new nomenclature for CS-like proteins in plants is also proposed.  (+info)

Cysteine synthase (O-acetylserine (thiol) lyase) substrate specificities classify the mitochondrial isoform as a cyanoalanine synthase. (8/159)

A cyanoalanine synthase and two isoforms (A, cytosolic and B, chloroplastic) of cysteine synthase (O:-acetylserine (thiol) lyase) were isolated from spinach. N-terminal amino acid sequence analysis of the cyanoalanine synthase gave 100% homology for the determined 12 residues with a published sequence for the mitochondrial cysteine synthase isoform. All three enzymes catalysed both the cysteine synthesis and cyanoalanine synthesis reactions, although with different efficiencies. Michaelis-Menten kinetics were observed for all three enzymes when substrate saturation experiments were performed varying O:-acetylserine, chloroalanine and cysteine. Negative co-operative kinetics were observed for cysteine synthases A and B when substrate saturation experiments were performed varying sulphide and cyanide, compared with the Michaelis-Menten kinetics observed for cyanoalanine synthase. The exception was negative co-operativity observed towards sulphide for cyanoalanine synthase with O:-acetylserine as co-substrate. The optimum sulphide concentration was dependent on the alanyl co-substrate used. The amino acid sequence similarity places these three enzymes in the same gene family, and whilst the close kinetic similarities support this, they also indicate distinct roles for the isoforms.  (+info)

Author: Hesse, H. et al.; Genre: Journal Article; Published in Print: 1999; Keywords: amino acids|br/|targeting|br/|mitochondria|br/|chloroplasts|br/|cysteine synthase|br/|transit peptide|br/|transgenic plants|br/|processing|br/|acetylserine thiol lyase|br/|transgenic tobacco|br/|spinacia-oleracea|br/|cdna cloning|br/|precursor proteins|br/|escherichia-coli|br/|datura-innoxia|br/|plant-tissues|br/|chloroplasts|br/|localization; Title: Molecular cloning and expression analyses of mitochondrial and plastidic isoforms of cysteine synthase (O-acetylserine(thiol)lyase) from Arabidopsis thaliana
Cysteine synthase C1; Acts as a major beta-cyanoalanine synthase. The cyanoalanine synthesis reaction is more efficient than the cysteine synthase activity. Probably unable to interact with SAT and to form the decameric Cys synthase complex (CSC) and is therefore not an enzymatically true OASTL protein. Probably involved in the detoxification of cyanide that arises from ethylene biosynthesis. Maintains a low level of cyanide for proper root hair development (368 aa ...
1FCJ: Identification of an allosteric anion-binding site on O-acetylserine sulfhydrylase: structure of the enzyme with chloride bound.
1FCJ: Identification of an allosteric anion-binding site on O-acetylserine sulfhydrylase: structure of the enzyme with chloride bound.
Parental exposure to pathogens can prime offspring immunity in diverse organisms. The mechanisms by which this heritable priming occurs are largely unknown. Here we report that the soil bacteria Pseudomonas vranovensis is a natural pathogen of the nematode Caenorhabditis elegans and that parental exposure of animals to P. vranovensis promotes offspring resistance to infection. Furthermore, we demonstrate a multigenerational enhancement of progeny survival when three consecutive generations of animals are exposed to P. vranovensis. By investigating the mechanisms by which animals heritably adapt to P. vranovensis infection, we found that parental infection by P. vranovensis results in increased expression of the cysteine synthases cysl-1 and cysl-2 and the regulator of hypoxia inducible factor rhy-1 in progeny, and that these three genes are required for adaptation to P. vranovensis. These observations establish a CYSL-1, CYSL-2, and RHY-1 dependent mechanism by which animals heritably adapt to ...
The cysB gene of Klebsiella aerogenes has been cloned, sequenced and shown to complement the cysteine auxotrophic phenotype of Escherichia coli cysB mutants. The K. aerogenes cysB gene is predicted to encode a protein of 324 amino acid residues that shares approx. 95% sequence similarity with the Salmonella typhimurium and E. coli CysB proteins. Gel-retardation assays demonstrate that the purified protein binds to DNA fragments containing either the K. aerogenes cysb promoter or the S. typhimurium cysJIH promoter. Acetylserine enhances CysB binding to the cysJIH promoter fragment while diminishing its binding to the cysB promoter fragment. Fluorescence-emission-spectroscopy measurements suggest strongly that N-acetylserine binds to CysB apoprotein but that O-acetylserine does not, and support the notion that N-acetylserine is the physiological inducer of cysteine biosynthesis.. ...
Residues 187-311 are 76% similar to a (CYSTEINE SYNTHASE O-ACETYLSERINE LYASE SULFHYDRYLASE) protein domain (PD001075) which is seen in Q9CKD0_PASMU ...
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PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the formation of a CymR-CysK complex and transcriptional repression of the CymR regulon occurs. In the absence of cysteine, the OAS pool is high and the CymR-CysK complex is mostly dissociated, leading to a faster dissociation of CymR from its DNA targets and the lifting of CymR-dependent repression ...
ITC analysis of the interaction between substrate, OAS (O-acetylserine) and OASS. Data is plotted as heat signal (μJ/sec) versus time (min) in the upper panel
Grundy FJ, Henkin TM (1998) The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria. Mol Microbiol 30:737-49.[PMID:10094622 ...
Grundy FJ, Henkin TM (1998) The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria. Mol Microbiol 30:737-49.[PMID:10094622 ...
(Microbial infection) In addition to its role in cysteine synthesis, stimulates the tRNase activity of CdiA-CT from E.coli strain 536 / UPEC; stimulation does not require O-acetylserine sulfhydrylase activity. CdiA is the toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion (experiments done in strains BW25113 and X90, both K12 derivatives). This protein is not required for CDI of strain EC93, whose toxin may function by forming inner cell membrane pores (PubMed:22333533). CysK stabilizes CdiA-CT, allowing it to bind tRNA substrate; neither CdiA-CT nor CysK bind tRNA alone in vitro (PubMed:27531961).
TY - JOUR. T1 - Two pathways for cysteine biosynthesis in Leishmania major. AU - Williams, Roderick A. M.. AU - Westrop, Gareth D.. AU - Coombs, Graham H.. PY - 2009/6/15. Y1 - 2009/6/15. N2 - Genome mining and biochemical analyses have shown that Leishmania major possesses two pathways for cysteine synthesis - the de novo biosynthesis pathway comprising SAT (serine acetyltransferase) and CS (cysteine synthase) and the RTS (reverse trans-sulfuration) pathway comprising CBS (cystathionine beta-synthase) and CGL (cystathionine gamma-lyase). The LmjCS (L. major CS) is similar to the type A CSs of bacteria and catalyses the synthesis of cysteine using O-acetylserine and Sulfide with K(m)s of 17.5 and 0.13 mm respectively. LmjCS can use sulfide provided by the action of MST (mercaptopyruvate Sulfurtransferase) oil 3-MP (3-mercaptopyruvate). LmJCS forms a bi-enzyme complex with Leishmania SAT (and Arabidopsis SAT), with residues LYs(222), His(226) and Lys(227) of LmjCS being involved in the complex ...
O-acetylhomoserine O-acetylserine sulfhydrylase; involved in sulfur amino acid biosynthesis; immunogenic; Hog1p; biofilm; Predicted ORF from Assembly 19; Gcn4p-regulatedProtein abundance is affected by URA3 expression in the CAI-4 strain background; alkaline upregulated; Gcn4p-regulatedProtein described as aspartate aminotransferase; soluble protein in hyphae; alkaline upregulated; amphotericin B repressed Malic enzyme, mitochondrial protein; transcription regulated by Mig1p and Tup1p; shows colony morphology-related gene Predicted ORF from Assembly 19; Gcn4p-regulated Large subunit of heterodimeric alpha-aminoadipate reductase; enzyme of lysine biosynthesis; contains predicted binding sites for AMP and alpha-aminoadipate; feedback inhibited by lysine or Putative NAD-specific glutamate dehydrogenase; fungal-specific (no human or murine homolog); transcription is regulated by Nrg1p, Mig1p, Tup1p, and Gcn4pSimilar to an aldose 1-epimerase-related protein; antigenic during murine systemic ...
Accepted name: cysteate synthase. Reaction: O-phospho-L-serine + sulfite = L-cysteate + phosphate. Other name(s): sulfite:O-phospho-L-serine sulfotransferase (phosphate-hydrolysing, L-cysteate-forming).. Systematic name: sulfite:O-phospho-L-serine sulfonotransferase (phosphate-hydrolysing, L-cysteate-forming).. Comments: A pyridoxal-phosphate protein. It is highly specific for O-phospho-L-serine and sulfite. The reaction proceeds through a dehydroalanine (2-aminoacrylic acid) intermediate. The enzyme from Methanosarcina acetivorans is evolutionarily related to threonine synthase (EC 4.2.3.1), but the reaction is more similar to that of O-phosphoserine sulfhydrylase (EC 2.5.1.65).. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: References:. 1. Graham, D.E., Taylor, S.M., Wolf, R.Z. and Namboori, S.C. Convergent evolution of coenzyme M biosynthesis in the Methanosarcinales: cysteate synthase evolved from an ancestral threonine synthase. Biochem. J. 424 (2009) ...
Autor: Krueger, S. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2009; Keywords: non-aqueous fractionation|br/|organelles|br/|regulation|br/|subcellular|br/|sulphur metabolism|br/|acetylserine thiol lyase|br/|synthase protein complex|br/|amino-acids|br/|gene family|br/|spinach leaves|br/|potato-tubers|br/|higher-plants|br/|salmonella-typhimurium|br/|sulfate assimilation|br/|hydrogen-sulfide; Titel: Analysis of cytosolic and plastidic serine acetyltransferase mutants and subcellular metabolite distributions suggests interplay of the cellular compartments for cysteine biosynthesis in Arabidopsis
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Although inflammation and protease/antiprotease imbalance have been postulated to be critical in cigarette smokeCinduced (CS-induced) emphysema, oxidative stress has been suspected to play an important role in chronic obstructive pulmonary diseases. transcription factor which, upon activation in response to oxidative or electrophilic stress, detaches from its cytosolic inhibitor, Keap1, translocates to the nucleus, and binds to the antioxidant response element (ARE) in the promoter of target genes, leading to their transcriptional induction (13). Though little is known about Nrf2-regulated genes in the lungs, the recognized members of this group include several critical antioxidant genes, such as heme oxygenase-1 (HO-1), Cglutamyl cysteine synthase (-GCS), and several members of the glutathione S-transferase (GST) family (13). We have postulated that Nrf2 is a critical transcription factor that determines susceptibility to lung inflammation, oxidative stress, and alveolar cell apoptosis caused ...
Este trabalho objetivou avaliar o uso combinado de sensores Lidar e RGB em ARP para quantificar as perturbações decorrentes da extração seletiva em área de manejo florestal. O estudo foi feito em uma área de exploração na Floresta Estadual do Antimary (FEA), no Estado do Acre. Foram utilizadas 10 parcelas de 1ha, para estimar biomassa seca acima do solo (BSAS) para toda área a partir de métricas extraídas do voo Lidar realizado antes da exploração. O voo ARP ocorreu após a exploração para classificação e quantificação das áreas impactadas por clareiras, estradas e pátios. A área de impacto total representou 13 % da área do estudo e pela perda de BSAS 31,5 Mg.ha-1, destes, as clareiras corresponderam a 82% da área total impactada e por 26,1 Mg.ha-1 da BSAS ...
Hopkins, L.; Parmar, S.; Baszczyk, A.; Hesse, H.; Hoefgen, R.; Hawkesford, M. J.: O-acetylserine and the regulation of expression of genes encoding components for sulfate uptake and assimilation in potato. Plant Physiology 138 (1), pp. 433 - 440 (2005 ...
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The coding sequence of the wild-type, cys-sensitive, cysE gene from Escherichia coli, which encodes an enzyme of the cysteine biosynthetic pathway, namely serine acetyltransferase (SAT, EC 2.3.1. 30), was introduced into the genome of potato plants under the control of the cauliflower mosaic virus 3 …
Cysteine occupies a central position in plant metabolism due to its biochemical functions. Arabidopsis thaliana cells contain different O-acetylserine(thiol)lyase (OASTL) enzymes that catalyze the biosynthesis of cysteine. Because they are localized in the cytosol, plastids and mitochondria, this results in multiple subcellular cysteine pools. Much progress has been made on the most abundant OASTL enzymes; however, information on the less abundant OASTL-like proteins has been scarce. To unequivocally establish the enzymatic reaction catalyzed by the minor cytosolic OASTL isoform CS-LIKE (AT5G28030), we expressed this enzyme in bacteria and characterized the purified recombinant protein. Our results demonstrate that CS-LIKE catalyzes the desulfuration of L-cysteine to sulfide plus ammonia and pyruvate. Thus, CS-LIKE is a novel L-cysteine desulfhydrase (EC 4.4.1.1), and we propose to designate it DES1. The impact and functionality of DES1 in cysteine metabolism was revealed by the phenotype of the T-DNA
Lithgow et al. showed that inactivation of the cysteine synthase CysM in S. aureus SH1000 results in decreased resistance against oxidative stress (48). Furthermore, they detected a depleted cysteine pool in the cysM-lacking mutant. The diminished oxidative stress resistance is probably linked to this depletion. Here we provide evidence that cysteine participates in the formation of S thiolations in S. aureus, as revealed by the [35S]cysteine assay and the GeLC-MS approach. Thus, it is likely that cysteine functions as thiol redox buffer in S. aureus and B. subtilis. BSH, another abundant cysteine-derived thiol in both organisms, is most likely also a key player in this buffer (52). CoA may also contribute to this buffer, which is an abundant thiol in S. aureus (49). Moreover, S. aureus possesses a CoA-disulfide reductase which strengthens a possible role of CoA as thiol redox-buffer (14, 15).. Using the diagonal gel electrophoresis, we confirmed that redox-active enzymes, such as AhpC and Tpx, ...
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O-phospho-L-serine---tRNA ligase ( EC 6.1.1.27 , O-phosphoseryl-tRNA ligase, non-canonical O-phosphoseryl-tRNA synthetase, SepRS) is an enzyme with systematic name O-phospho-L-serine:tRNACys ligase (AMP-forming). This enzyme catalyses the following chemical reaction : In organisms like Archaeoglobus fulgidus , this enzyme ligates O-phosphoserine to tRNACys. References Fukunaga, R.; Yokoyama, S. (2007).
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L-Cysteine is a crystalline free-form amino acid that is active in many body processes. L-Cysteine works in the liver to protect the body from harmful substance
دیابت ملیتوس (DM) از بیماری-های متابولیک بوده که با عوارض متعدد خونی و بافتی همراه است. هدف از مطالعه حاضر بررسی تغییرات پلاسمایی سطح تروپونین قلبی I (cTnI)، هموسیستئین (Hcy)، سیستاتین C (Cys-C) و فعالیت آدنوزین دِآمیناز (ADA) در دیابت ملیتوس القاء شده با آلوکسان در سگ می-باشد. در این مطالعه، پس از القاء دیابت ملیتوس در گروه تیمار و پس از سه ماه نگه-داری، سطح سرمی cTnI، Cys-C و Hcy و فعالیت ADA با استفاده از تکنیک الایزا و الکتروکمی لومینسانس (ECL) در پلاسما اندازه-گیری و با گروه سالم مقایسه گردید. در پایان، افزایش معنی-دار (01/0p|) در سطوح پلاسمایی cTnI، Cys-C و Hcy همراه با کاهش معنی--دار (01/0p|)
L-cysteine Hydrochloride Review, Benefits, Side Effects and Uses. Anhydrous and Monohydrate formulas for solubility plus dosages and how to take.
No, I have not got an HHO generator installed yet, but of course I am interested in the possibility of doing so and I am going to buy one, I am planning to buy one or I am considering buying one ...
A 0·5 kb fragment of Streptomyces venezuelae ISP5230 genomic DNA was amplified by PCR using primers based on consensus sequences of cysteine synthase isozyme A from bacteria. The deduced amino acid sequence of the PCR product resembled not only cysteine synthase sequences from prokaryotes and eukaryotes but also eukaryotic cystathionine β-synthase sequences. Probing an Str. venezuelae genomic library with the PCR product located a hybridizing colony from which pJV207 was isolated. Sequencing and analysis of the Str. venezuelae DNA insert in pJV207 detected two ORFs. The deduced amino acid sequence of ORF1 matched both cysteine synthase and cystathionine β-synthase sequences in GenBank, but its size favoured assignment as a cystathionine β-synthase. ORF2 in the pJV207 insert was unrelated in function to ORF1; in its sequence the deduced product resembled acetyl-CoA transferases, but disruption of the ORF did not cause a detectable phenotypic change. Disruption of ORF1 failed to elicit cysteine
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NAC is a more stable form of L-Cysteine because it has an acetyl group (CH3CO) attached. NAC has all the properties of L-Cysteine but is more water soluble and said to be more bioavailable than L-Cysteine. NAC may be the most cost effective way to boost Glutathione levels in the body. Jo Mars NAC is 100% pure U.S.P. g
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This study investigated the effect of Midostaurin on ascites and on other symptoms in patients with Aggressive Systemic Mastocytosis.
Autor: Riemenschneider, A. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2005; Keywords: arabidopsis thaliana|br/|cysteine|br/|desulfhydrase|br/|h2s|br/|o-acetyl-l-serine|br/|acetylserine thiol lyase|br/|arabidopsis-thaliana|br/|brassica-oleracea|br/|atmospheric h2s|br/|sulfur source|br/|protein|br/|plants|br/|mitochondrial|br/|sulfide|br/|biosynthesis; Titel: Impact of elevated H2S on metabolite levels, activity of enzymes and expression of genes involved in cysteine metabolism
The 5′-triphosphorylated, 2′-5′-linked oligoadenylate polyribonucleotides (2-5As) are central to the interferon-induced antiviral 2-5A system. The 2-5As bind and activate the RNase L, an endoRNase degrading viral and cellular RNA leading to inhibition of viral replication. The 2-5A system is tightly controlled by synthesis and degradation of 2-5As. Whereas synthesis is mediated by the 2′-5′ oligoadenylate synthetase family of enzymes, degradation seems to be orchestrated by multiple enzyme nucleases including phosphodiesterase 12, the ectonucleotide pyrophosphatase/phosphodiesterase 1 and the A-kinase anchoring protein 7. Here we present assay tools for identification and characterization of the enzymes regulating cellular 2-5A levels. A procedure is described for the production of 2′-5′ oligoadenylates, which are then used as substrates for development and demonstration of enzyme assays measuring synthetase and nuclease activities, respectively. The synthetase assays produce only a single
Genotype networks, innovation, and robustness in sulfur metabolism - Quantitative Biology > Molecular Networks. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Siderophores are essential factors for iron (Fe) acquisition in bacteria during colonization and infection of eukaryotic hosts, which restrain iron access through iron-binding protein, such as lactoferrin and transferrin. The synthesis of siderophores by Escherichia coli is considered to be fully regulated at the transcriptional level by the Fe-responsive transcriptional repressor Fur. Here we characterized two different pathways that promote the production of the siderophore enterobactin via the action of the small RNA RyhB. First, RyhB is required for normal expression of an important enterobactin biosynthesis polycistron, entCEBAH. Second, RyhB directly represses the translation of cysE, which encodes a serine acetyltransferase that uses serine as a substrate for cysteine biosynthesis. Reduction of CysE activity by RyhB allows serine to be used as building blocks for enterobactin synthesis through the nonribosomal peptide synthesis pathway. Thus, RyhB plays an essential role in siderophore ...
2) 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. For diagram, click here or here or here for mechanism click here.. Other name(s): PSAT; phosphoserine aminotransferase; 3-phosphoserine aminotransferase; hydroxypyruvic phosphate-glutamic transaminase; L-phosphoserine aminotransferase; phosphohydroxypyruvate transaminase; phosphohydroxypyruvic-glutamic transaminase; phosphoserine aminotransferase; 3-O-phospho-L-serine:2-oxoglutarate aminotransferase; SerC; PdxC; 3PHP transaminase. Systematic name: O-phospho-L-serine:2-oxoglutarate aminotransferase. Comments: A pyridoxal-phosphate protein. This enzyme catalyses the second step in the phosphorylated pathway of serine biosynthesis in Escherichia coli [2,3]. It also catalyses the third step in the biosynthesis of the coenzyme pyridoxal 5-phosphate in Escherichia coli (using Reaction 2 above) [3]. In Escherichia coli, pyridoxal 5-phosphate is synthesized de novo by a pathway that involves EC ...
This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name of this enzyme class is O-phosphoserine phosphohydrolase. This enzyme participates in glycine, serine and threonine metabolism. ...
L-Cysteine is a crystalline free-form amino acid that is active in many body processes. L-Cysteine works in the liver to protect the body from harmful substances and may help to prevent oxidation of tissues. L-Cysteine, along with L-Glutamic Acid and Glycine, is the rate-limiting precursor in the synthesis of glutathio
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This graph shows the total number of publications written about Cysteine by people in this website by year, and whether Cysteine was a major or minor topic of these publications ...
This conversion requires the enzyme glutamate-cysteine ligase (GCL, glutamate cysteine synthase). This reaction is the rate- ... It plays a role in the storage of cysteine. Glutathione enhances the function of citrulline as part of the nitric oxide cycle. ... It is a tripeptide with a gamma peptide linkage between the carboxyl group of the glutamate side chain and cysteine. The ... Ha SB, Smith AP, Howden R, Dietrich WM, Bugg S, O'Connell MJ, Goldsbrough PB, Cobbett CS (June 1999). "Phytochelatin synthase ...
This is a byproduct of the metabolism of cysteine by cystathionine-b-synthase. Like with NO, inhalation of H2S has been found ... Nitric Oxide (NO) is a byproduct of the metabolism of arginine to citrulline by NO synthase. This gas is available in all cells ...
Cysteine synthase, for example, catalyzes the formation of acetic acids and cysteine from O3-acetyl-L-serine and hydrogen ... elegans aging is modulated by hydrogen sulfide and the sulfhydrylase/cysteine synthase cysl-2". PLOS ONE. 8 (11): e80135. ... Selenocysteine synthase, which was first added to the classification system in 1999, converts seryl-tRNA(Sec UCA) into ... An example of a prominent glycosyltransferase is lactose synthase which is a dimer possessing two protein subunits. Its primary ...
Hondorp, Elise R.; Matthews, Rowena G. (May 2009). "Oxidation of cysteine 645 of cobalamin-independent methionine synthase ... Drennan, C. L.; Matthews, R. G.; Ludwig, M. L. (December 1994). "Cobalamin-dependent methionine synthase: the structure of a ... methionine synthase, a B-12 dependent enzyme in humans, and methylenetetrahydrofolate reductase. Her collaboration with ... cobalamin-dependent methionine synthase. A love affair with vitamins (2009) Cobalamin- and corrinoid-dependent enzymes ...
"Organisation of the yeast ATP synthase F(0):a study based on cysteine mutants, thiol modification and cross-linking reagents". ... "ATP synthase protein 8". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB). "MT-ATP8 mitochondrially encoded ATP synthase ... "The molecular neighborhood of subunit 8 of yeast mitochondrial F1F0-ATP synthase probed by cysteine scanning mutagenesis and ... a type of naturally-derived antibiotic that is able to inhibit the Fo unit of ATP synthase. The Fo region of ATP synthase is a ...
Codexis). Another example is the possibility of using the promiscuous activities of cysteine synthase (cysM) towards ... promiscuity can be decreased as was the case of γ-humulene synthase (a sesquiterpene synthase) from Abies grandis that is known ... including overexpression of the large component of a synthase in the absence of the amine transferase subunit), pathway bypass ... "Semisynthetic production of unnatural L-alpha-amino acids by metabolic engineering of the cysteine-biosynthetic pathway". ...
Transfer of a cysteine synthase from a bacterium into phytophagous mites and Lepidoptera allowing the detoxification of ...
... a putative cysteine synthase and sul, a putative dihydropteroate synthase. In Listeria monocytogenes four additional copies of ...
It is synthesized by the addition and oxidation of cysteine to histidine by 5-histidylcysteine sulfoxide synthase, followed by ...
... encoded cysteine synthase. and the metZ or metY -encoded homocysteine synthase, Weekley, C. M. and Harris, H. H. (2013). "Which ... Cystathionine γ-synthase (metB) which joins an activated homoserine ether (acetyl or succinyl) with cysteine to form ... with homocysteine to form cystathionine Not Cystathionine β-synthase which is a PLP enzyme type II cysteine biosynthesis from ... In Klebsiella pneumoniae the cystathionine β-synthase is encoded by mtcB, while the γ-lyase is encoded by mtcC. Humans are ...
... and pyridoxal phosphate utilizing enzymes such as cysteine synthase. Upon nucleotide hydrolysis the loop does not significantly ... The conserved sequence of this motif is C-x(5)-R-[ST], where C and R denote cysteine and arginine residues respectively. The A- ... These include ATP synthase (α and β subunits), myosin, transducin, helicases, kinases, AAA proteins, G-proteins, RecA, protein ... and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold". EMBO ...
Small amounts of biosynthetic enzymes L-cysteine synthase and acetyltransferase were detected in L. jordanis and L. pneumophila ...
"Mechanisms of mitochondrial holocytochrome c synthase and the key roles played by cysteines and histidine of the heme ... 2006). "Mutations of the mitochondrial holocytochrome c-type synthase in X-linked dominant microphthalmia with linear skin ... "Mutations of the mitochondrial holocytochrome c-type synthase in X-linked dominant microphthalmia with linear skin defects ... "Conserved residues of the human mitochondrial holocytochrome c synthase mediate interactions with heme". Biochemistry. 53 (32 ...
Mammals biosynthesize the amino acid cysteine via homocysteine. Cystathionine β-synthase catalyses the condensation of ... In the body, homocysteine can be recycled into methionine or converted into cysteine with the aid of certain B-vitamins. A high ... It is a homologue of the amino acid cysteine, differing by an additional methylene bridge (-CH2-). It is biosynthesized from ... More detail on these enzymes can be found in the article for methionine synthase. Homocysteine can cyclize to give homocysteine ...
The enzyme cysteine synthase, using sulfide sources, converts this ester into cysteine, releasing acetate. The cysteine ... l-Cysteine is also used as a processing aid for baking. In the field of personal care, cysteine is used for permanent-wave ... N-Acetyl-l-cysteine is a derivative of cysteine wherein an acetyl group is attached to the nitrogen atom. This compound is sold ... Again, the cysteine is used for breaking up the disulfide bonds in the hair's keratin. Cysteine is a very popular target for ...
"Solvent-Accessible Cysteines in Human Cystathionine β-Synthase: Crucial Role of Cysteine 431 inS-Adenosyl-l-methionine Binding ... An example of such a conformational disease is ALAD porphyria, which results from a mutation of porphobilinogen synthase that ... The one protein that is established to function as a morpheein is porphobilinogen synthase, though there are suggestions ... An inhibitor of porphobilinogen synthase with this mechanism of action has been documented. The morpheein model of allosteric ...
"Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum". Phytochemistry 26: 2699-2704. ... "Enzymatic-synthesis of the neuroexcitatory amino-acid quisqualic by cysteine synthase". Phytochemistry 25: 2759-2763. ...
The N-terminal domain is a cysteine, histidine-dependent aminohydrolase amidase. Structurally the synthetase and amidase ... Targeting trypanothione synthase could be a novel way of preventing and curing these diseases through disruption of the ... Evidence for this regulation is that the residues which allow the synthase domain to block the amidase active site are highly ... The main function of trypanothione synthase is to use the free energy generated from ATP hydrolysis to conjugate glutathione ...
"Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum". Phytochemistry. 26 (10): 2699-2704. doi: ... In enzymology, a cysteine synthase (EC 2.5.1.47) is an enzyme that catalyzes the chemical reaction O3-acetyl-L-serine + ... cysteine synthetase, S-sulfocysteine synthase, 3-O-acetyl-L-serine:hydrogen-sulfide, and 2-amino-2-carboxyethyltransferase. ... "Enzymatic-synthesis of the neuroexcitatory amino-acid quisqualic by cysteine synthase". Phytochemistry. 25 (12): 2759-2763. doi ...
The characteristic reaction of β-ketoacyl-ACP synthase III is malonyl-ACP + acetyl-CoA => acetoacyl-ACP + CO2 + CoA. Cysteine, ... the two most common 3-ketoacyl-ACP synthase I and synthase II. 3-Ketoacyl-ACP synthase I (E.C. 2.3.1.41) is involved in the ... The dominant enzyme in the KS1 family is 3-ketoacyl-ACP synthase III (KAS III), also known as 3-oxoacyl-ACP synthase III and β- ... Chalcone synthase (E.C. 2.3.1.74), also known as naringenin-chalcone synthase, is responsible for the reaction: 3 malonyl-CoA ...
"Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum". Phytochemistry. 26 (10): 2699-2704. doi: ... In enzymology, an uracilylalanine synthase (EC 2.5.1.53) is an enzyme that catalyzes the chemical reaction O3-acetyl-L-serine ... Ahmmad MAS; Maskall CS; Brown EG (1984). "Partial-purification and properties of willardiine and synthase activity from Pisum ... Other names in common use include O3-acetyl-L-serine acetate-lyase (adding uracil), isowillardiine synthase, willardiine ...
... cystathionine synthase, cystathionine synthetase, homoserine transsuccinylase, 4-O-succinyl-L-homoserine:L-cysteine, and S-(3- ... Cystathionine gamma-synthase from plants uses L-homoserine phosphate instead. This enzyme belongs to the family of transferases ... The systematic name of this enzyme class is O4-succinyl-L-homoserine:L-cysteine S-(3-amino-3-carboxypropyl)transferase. Other ... In enzymology, a cystathionine gamma-synthase (EC 2.5.1.48) is an enzyme that catalyzes the formation of cystathionine from ...
... adding thioglycolate S-carboxymethyl-L-cysteine-forming). This enzyme is also called S-carboxymethyl-L-cysteine synthase. It ... In enzymology, a S-carboxymethylcysteine synthase (EC 4.5.1.5) is an enzyme that catalyzes the chemical reaction 3-chloro-L- ... Kumagai H, Suzuki H, Shigematsu H, Tuchikura T (1989). "S-Carboxymethylcysteine synthase from Escherichia coli". Agric. Biol. ... whereas its two products are S-carboxymethyl-L-cysteine and chloride. This enzyme belongs to the family of lyases, specifically ...
"Evidence for identity of beta-pyrazolealanine synthase with cysteine synthase in watermelon: formation of beta-pyrazole-alanine ... Other names in common use include beta-(1-pyrazolyl)alanine synthase, beta-pyrazolealanine synthase, beta-pyrazolylalanine ... synthase (acetylserine), O3-acetyl-L-serine acetate-lyase (adding pyrazole), BPA-synthase, pyrazolealanine synthase, ... by cloned cysteine synthase in vitro and in vivo". Biochem. Biophys. Res. Commun. 197 (3): 1111-7. doi:10.1006/bbrc.1993.2592. ...
... cysteine synthase, L-serine hydro-lyase (adding homocysteine), methylcysteine synthase, serine sulfhydrase, and serine ... The lack of CBS in these tissues implies that these tissues are unable to synthesize cysteine and that cysteine must be ... Cystathionine-β-synthase, also known as CBS, is an enzyme (EC 4.2.1.22) that in humans is encoded by the CBS gene. It catalyzes ... Methylcysteine synthase was assigned the EC number EC 4.2.1.23 in 1961. A side-reaction of CBS caused this. The EC number EC ...
Ramamurthy V, Swann SL, Spedaliere CJ, Mueller EG (October 1999). "Role of cysteine residues in pseudouridine synthases of ... TRNA pseudouridine32 synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic ... TRNA+pseudouridine32+synthase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J (June 1995). "A dual-specificity pseudouridine synthase: an Escherichia ...
... cysteine desulfurase]-S-sulfanyl-L-cysteine ⇌ {\displaystyle \rightleftharpoons } AMP + [molybdopterin-synthase sulfur-carrier ... molybdopterin synthase sulfurylase) is an enzyme with systematic name persulfurated L-cysteine desulfurase:(molybdopterin- ... Molybdopterin+synthase+sulfurtransferase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal ... Leimkühler S, Rajagopalan KV (June 2001). "A sulfurtransferase is required in the transfer of cysteine sulfur in the in vitro ...
The gas is produced from cysteine by the enzymes cystathionine beta-synthase and cystathionine gamma-lyase. It acts as a ... Alp, Nicholas; Channon (2003). "Regulation of endothelial nitric oxide synthase by tetrahydrobiopterin in vascular disease". ... "Hydrogen sulfide cytoprotective signaling is endothelial nitric oxide synthase-nitric oxide dependent". PNAS. 111 (Early ... accumulation occurs due to increased electron transport uncoupling at the active site of endothelial nitric oxide synthase ( ...
The gas is produced from cysteine by the enzymes cystathionine beta-synthase and cystathionine gamma-lyase. It acts as a ... In plants, nitric oxide can be produced by any of four routes: (i) L-arginine-dependent nitric oxide synthase, (although the ... Phagocytes are armed with inducible nitric oxide synthase (iNOS), which is activated by interferon-gamma (IFN-γ) as a single ... SAM is then converted to 1-aminocyclopropane-1-carboxylic acid (ACC) by the enzyme ACC synthase (ACS). The activity of ACS ...
The generation of animals that lack both Endothelium Nitric Oxide Synthase (eNOS) and COX-1 (Cyclooxygenase-1, a protein that ... it was suggested that EDHF is a sulfur signal that results in activation of K channels via sulfhydration of a cysteine residue ... formation of a cysteine persulfide) (https://doi.org/10.1161/CIRCRESAHA.111.240242) ...
GMP synthase. *Asparagine synthetase. *Carbamoyl phosphate synthetase *I. *II. *Glutamate-cysteine ligase ...
All cytochrome c proteins contain a characteristic CXXCH (cysteine-any-any-cysteine-histidine) amino acid motif that binds heme ... activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c. • cellular respiration. • ... This release of cytochrome c in turn activates caspase 9, a cysteine protease. Caspase 9 can then go on to activate caspase 3 ... the heme group of cytochrome c makes thioether bonds with two cysteine side chains of the protein.[15] One of the main ...
... or by either LTC4 synthase or microsomal glutathione S-transferase 2 (MGST2), which bind the sulfur of cysteine's thio (i.e. SH ... also termed LTA4 synthase) activity which converts 5S-HpETE to its epoxide, 5S,6S-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid ( ... by LTC4 synthase (LTC4S); LTC4 exits its cells of origin through the MRP1 transporter (ABCC1) and is rapidly converted to LTD4 ... residue in the tripeptide glutamate-cysteine-glycine to carbon 6 of LTA4 thereby forming LTC4 (i.e. 5S-hydroxy,6R-(S- ...
In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) ...
This entry also includes folylpolyglutamate synthase that transfers glutamate to folylpolyglutamate and cyanophycin synthetase ... GMP synthase. *Asparagine synthetase. *Carbamoyl phosphate synthetase *I. *II. *Glutamate-cysteine ligase ...
Recent advances in buffering technology alleviate this problem by resolving the proteins at a pH well below the pKa of cysteine ... "FRET reveals changes in the F1-stator stalk interaction during activity of F1F0-ATP synthase". Biochim Biophys Acta. 1607 (2-3 ... A drawback of this system is that these pH values may promote disulfide bond formation between cysteine residues in the ... proteins because the pKa of cysteine ranges from 8-9 and because reducing agent present in the loading buffer doesn't co- ...
positive regulation of nitric-oxide synthase biosynthetic process. • MyD88-dependent toll-like receptor signaling pathway. • ... and tri-acylated cysteine-containing lipopeptides. It does this by forming dimeric complexes with either TLR 1 or TLR6 on the ...
It is interesting to note that, when Esa1 is assembled in the piccolo NuA4 complex, it loses its dependence on the cysteine ... Aminolevulinic acid synthase. *Beta-ketoacyl-ACP synthase. *Glyceronephosphate O-acyltransferase. *Lecithin-cholesterol ... Then, a glutamate residue acts as a general base to facilitate transfer of the acetyl group from the cysteine to the histone ... Many MYST proteins also contain a cysteine-rich, zinc-binding domain within the HAT region in addition to an N-terminal ...
PLP aids in the synthesis of hemoglobin, by serving as a coenzyme for the enzyme ALA synthase.[6] It also binds to two sites on ... Vitamin B6 deficiency can also result in impaired transsulfuration of methionine to cysteine. The PLP-dependent transaminases ... PLP is a coenzyme needed for the proper function of the enzymes cystathionine synthase and cystathionase. These enzymes ... Part of this pathway (the reaction catalyzed by cystathionase) also produces cysteine. ...
... synthesis: Cystathionine beta synthase catalyzes the upper reaction and cystathionine gamma-lyase catalyzes the lower ... "cysteine - Definition of cysteine in English by Oxford Dictionaries". Oxford Dictionaries - English. Retrieved 15 April 2018.. ... Cysteine (symbol Cys or C;[3] /ˈsɪstiiːn/)[4] is a semiessential[5] proteinogenic amino acid with the formula HO2CCH(NH2)CH2SH ... For example, the reaction of cysteine with sugars in a Maillard reaction yields meat flavors.[26] L-Cysteine is also used as a ...
... triterpenoid synthases, epoxide hydrolases, cytochrome P450s, and UDP-glucosyltransferases.[6] The metabolic pathway for ... and cysteine.[8] ...
Youle, RJ; Huang, AHC (1981). "Occurrence of low molecular weight and high cysteine containing albumin storage proteins in ... synthase. The substituted version is not sensitive to glyphosate.[118] ...
These include inhibitors of oxidosqualene cyclase and squalene synthase,[68][69] cysteine protease inhibitors,[68][70] ... "Squalene synthase as a chemotherapeutic target in Trypanosoma cruzi and Leishmania mexicana". Mol Biochem Parasitol. 125 (1-2 ... "Cysteine Protease Inhibitors Cure an Experimental Trypanosoma cruzi Infection". J Exp Med. 188 (4): 725-34. doi:10.1084/jem. ...
conversion to cysteine: Cystathionine. *α-Ketobutyric acid+Cysteine. threonine→. *α-Ketobutyric acid ...
In DAHP synthase, it has been shown that binding site contains patterns of cysteine and histidine residues bound to metal ions ... DAH7-P synthase, DAHP synthase, DS-Co, DS-Mn, KDPH synthase, KDPH synthetase, deoxy-D-arabino-heptulosonate-7-phosphate ... The quaternary structure of DAHP synthase consists of two tightly bound dimers, which means that DAHP synthase is a tetramer.[5 ... To the right is an image of DAHP synthase that shows the quaternary structure of DAHP synthase. This image shows that DAHP ...
ATP synthase (complex V)Edit. Further information: ATP synthase. ATP synthase, also called complex V, is the final enzyme in ... Each iron atom in these clusters is coordinated by an additional amino acid, usually by the sulfur atom of cysteine. Metal ion ... as well as Efraim Racker on the ATP synthase.[104] A critical step towards solving the mechanism of the ATP synthase was ... through a large enzyme called ATP synthase; this process is known as chemiosmosis. The ATP synthase uses the energy to ...
The cysteine residue at 302 in ALDH1 and 200 in ALDH2 is implicated as a disulfiram binding site on the enzyme and serves as a ... Cysteine-302 is one of three consecutive Cys residues and is crucial to the enzyme's catalytic function. The residue is ... disfulfiram sensitive thiol site.[12] Covalent binding of disulfiram to the thiol blocks the binding of one of the cysteine ...
conversion to cysteine: Cystathionine beta synthase. *Cystathionine gamma-lyase. THREONINE→. *Threonine aldolase ...
Citrate synthase Aldol condensation This is the same as step 0 and restarts the cycle. The reaction is irreversible and extends ... De-aminated alanine, cysteine, glycine, serine, and threonine are converted to pyruvate and can consequently either enter the ... When tested in vitro with TCA enzymes, ATP inhibits citrate synthase and α-ketoglutarate dehydrogenase; however, ATP levels do ... Citrate synthase Aldol condensation irreversible, extends the 4C oxaloacetate to a 6C molecule ...
Carbamoyl phosphate synthase (CPSase) is a heterodimeric enzyme composed of a small and a large subunit (with the exception of ... Carbamoyl phosphate synthase has three main steps in its mechanism and is, in essence, irreversible.[4] ... "Inhibition of carbamoyl-phosphate synthase (ammonia) by Tris and Hepes. Effect on Ka for N-acetylglutamate" (PDF). Biochem. J ... GMP synthase. *Asparagine synthetase. *Carbamoyl phosphate synthetase *I. *II. *Glutamate-cysteine ligase ...
In the liver of vertebrates, glycine synthesis is catalyzed by glycine synthase (also called glycine cleavage enzyme). This ... conversion to cysteine: Cystathionine. *α-Ketobutyric acid+Cysteine. threonine→. *α-Ketobutyric acid ... The predominant pathway in animals and plants is the reverse of the glycine synthase pathway mentioned above. In this context, ... is biosynthesized from glycine and succinyl-CoA by the enzyme ALA synthase. Glycine provides the central C2N subunit of all ...
Moreover, the surface-exposed reduced cysteines could participate in disulfide crosslinking and, thus, aggregation.[7] ... Rotilio G, Aquilano K, Ciriolo MR (2004). "Interplay of Cu,Zn superoxide dismutase and nitric oxide synthase in ...
Cysteine HS-CH2- Serine → Cystathionine → α-ketobutyrate → Cysteine Asparagine H2N-CO-CH2- Aspartic Acid → Asparagine ( ... Homocysteine → Methionine (methionine synthase) Proline -(CH2)3- Glutamic Acid → Glutamate-5-semialdehyde → Proline (γ-glutamyl ... Disulfide bond formation is the creation of disulfide bridges (covalent bonds) between two cysteine amino acids in a chain ...
Villar-Palasí, C.; Guinovart, J. J. (1 June 1997). "The role of glucose 6-phosphate in the control of glycogen synthase". The ... cysteine, arginine and lysine in HeLa cell extracts [16]. However, due to the chemical lability of these phosphorylated ... The role of glucose 6-phosphate in glycogen synthase: High blood glucose concentration causes an increase in intracellular ... Allosteric activation by glucose 6 phosphate, which acts as an effector, stimulates glycogen synthase, and glucose 6 phosphate ...
Notable ones include thromboxane synthase (CYP5), prostacyclin synthase (CYP8), and CYP74A (allene oxide synthase). ... The iron is tethered to the protein via a cysteine thiolate ligand. This cysteine and several flanking residues are highly ... Examples include CYP5A1, thromboxane A2 synthase, abbreviated to TBXAS1 (ThromBoXane A2 Synthase 1), and CYP51A1, lanosterol 14 ... thromboxane A2 synthase. 1 subfamily, 1 gene. CYP5A1 CYP7. bile acid biosynthesis 7-alpha hydroxylase of steroid nucleus. 2 ...
SW, Ryter; J, Alam (April 2006). "Heme oxygenase-1/carbon monoxide: from basic science to therapeutic applications". Physiol Rev. Department of Medicine, Division of Pulmonary, Allergy, and Critical Care Medicine, The University of Pittsburgh School of Medicine. 86: 583-650. doi:10.1152/physrev.00011.2005. PMID 16601269 ...
Such incorporation requires reduction of cysteine residues within active site of SDHB. Both reduced cysteine residues and ... Secologanin synthase. *Tryptophan alpha,beta-oxidase. *Pyrroloquinoline-quinone synthase. *L-galactonolactone oxidase ...
"Difference between uracilylalanine synthases and cysteine synthases in Pisum sativum". Phytochemistry. 26 (10): 2699-2704. doi: ... In enzymology, a cysteine synthase (EC 2.5.1.47) is an enzyme that catalyzes the chemical reaction O3-acetyl-L-serine + ... cysteine synthetase, S-sulfocysteine synthase, 3-O-acetyl-L-serine:hydrogen-sulfide, and 2-amino-2-carboxyethyltransferase. ... "Enzymatic-synthesis of the neuroexcitatory amino-acid quisqualic by cysteine synthase". Phytochemistry. 25 (12): 2759-2763. doi ...
Cysteine synthesis is catalyzed by serine acetyltransferase (SAT) and O-acetylserine (thiol) lyase (OAS-TL) in the cytosol, ... Mitochondrial cysteine synthase complex regulates O-acetylserine biosynthesis in plants. *Wirtz M ... The presence of cysteine resulted in reduced OAS export in mitochondria of oastl-C mutants but not in WT mitochondria. This is ... Cysteine synthesis is catalyzed by serine acetyltransferase (SAT) and O-acetylserine (thiol) lyase (OAS-TL) in the cytosol, ...
Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is ... In the absence of cysteine, the OAS pool is high and the CymR-CysK complex is mostly dissociated, leading to a faster ... Catalyzes the conversion of O-acetylserine to cysteine. ... Belongs to the cysteine synthase/cystathionine beta-synthase ... Probable cysteine synthase (ytkP), Cysteine synthase (cysK). This subpathway is part of the pathway L-cysteine biosynthesis, ...
Microbial infection) In addition to its role in cysteine synthesis, stimulates the tRNase activity of CdiA-CT from E.coli ... Cysteine synthase B (cysM), Cysteine synthase (cysK), Cysteine synthase (cysM), Cysteine synthase A (cysK) ... cysteine synthase complex Source: EcoCycInferred from direct assayi*. "Effects of bienzyme complex formation of cysteine ... Belongs to the cysteine synthase/cystathionine beta-synthase family.Curated. Phylogenomic databases. evolutionary genealogy of ...
... Davis, Tatum V. ... A Mutator insertion was isolated in the cysteine synthase gene. An F2 population segregating for an albino phenotype and the ... cysteine synthase and chorismate mutase. One approach to define the importance of a gene is to isolate a mutation and examine ...
Modulation of cysteine biosynthesis in chloroplasts of transgenic tobacco overexpressing cysteine synthase. O-Acetylserine( ... Wirtz, M., Berkowitz, O., Droux, M., and Hell, R. (2001). The cysteine synthase complex from plants. Mitochondrial serine ... Noji, M., and Saito, K. (2002). Molecular and biochemical analysis of serine acetyltransferase and cysteine synthase towards ... Wirtz, M., and Hell, R. (2006). Functional analysis of the cysteine synthase protein complex from plants: Structural, ...
l-cysteine desulfhydrase Cysteine synthase Hydrogen sulfide Cadmium Glutathione Jie Shen, Ye Su, Can Zhou and Feng Zhang are ... A putative rice l-cysteine desulfhydrase encodes a true l-cysteine synthase that regulates plant cadmium tolerance. ... Beta-cyanoalanine synthase is a mitochondrial cysteine synthase-like protein in spinach and Arabidopsis. Plant Physiol 123:1163 ... Three Arabidopsis genes encoding proteins with differential activities for cysteine synthase and beta-cyanoalanine synthase. ...
Recombinant Protein and Bifunctional cystathionine gamma-lyase/cysteine synthase Antibody at MyBioSource. Custom ELISA Kit, ... Shop Bifunctional cystathionine gamma-lyase/cysteine synthase ELISA Kit, ... Bifunctional cystathionine gamma-lyase/cysteine synthase. Bifunctional cystathionine gamma-lyase/cysteine synthase ELISA Kit. ... Bifunctional cystathionine gamma-lyase/cysteine synthase Recombinant. Bifunctional cystathionine gamma-lyase/cysteine synthase ...
Hydrogen sulfide + O-acetyl-L-serine ↔ L-Cysteine + Acetic acid + hydron Hydrogen sulfide + O-acetyl-L-serine ↔ L-Cysteine + ...
Identification of the cysteine nitrosylation sites in human endothelial nitric oxide synthase. DNA and cell biology. 2008 Jan 1 ... Identification of the cysteine nitrosylation sites in human endothelial nitric oxide synthase. / Tummala, Monorama; Ryzhov, ... Identification of the cysteine nitrosylation sites in human endothelial nitric oxide synthase. ... We identified cysteines 660, 801, and 1113 as capable of undergoing S-nitrosylation. These cysteines are located within regions ...
... in the largest biology dictionary online. Free learning resources for students covering all major areas of ... Cysteine synthase. (Science: enzyme) An enzyme that catalyses the biosynthesis of cysteine in microorganisms and plants from o- ...
Kaushik, Abhishek (2017) Dissecting Regulatory Network in Cysteine Synthase Complex. PhD thesis, CSIR-IMTECH,Chandigarh/ ...
Structure of Leishmania major cysteine synthase. P. K. Fyfe, G. D. Westrop, T. Ramos, S. Müller, G. H. Coombs and W. N. Hunter ... Cover illustration: Structure of Leishmania major cysteine synthase (Fyfe et al., p. 738). ... A crystallographic and biochemical study of L. major cysteine synthase, which is a pyridoxyl phosphate-dependent enzyme, is ...
Cysteine synthases CYSL-1 and CYSL-2 mediate C. elegans heritable adaptation to P. vranovensis infection Caenorhabditis elegans ... a process mediated by the cysteine synthases CYSL-1 and CYSL-2. ...
... Show simple item ... Cysteine synthases CYSL-1 and CYSL-2 mediate C. elegans heritable adaptation to P. vranovensis infection.. ... vranovensis results in increased expression of the cysteine synthases cysl-1 and cysl-2 and the regulator of hypoxia inducible ...
Cysteine synthase. gi/16802269. 191. Metabolic processes. 38. ATP-dependent Clp protease proteolytic subunit. gi/16804506. 187 ... Other authors found that proteins like glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and cysteine synthase were induced in ... cysteine synthase, ATP-dependent Clp protease proteolytic subunit, transcription elongation factor GreA, hypothetical protein ...
Homocysteine and cysteine loads in patients with homocystinuria due to cystathionine synthase deficiency: effects of vitamin B- ... papers/homocysteine-and-cysteine-loads-in-patients-with/890952. ... of patients with homocystinuria due to cystabhionine synthase ... Cystathionine beta-synthase deficiency: a qualitative abnormality of the deficient enzyme modified by vitamin B6 therapy. ... Homocystinuria due to cystathionine synthase deficiency: enzymatic and ultrastructural studies. Mar 1, 1974·The Journal of ...
... aminocyclopropane-1-carboxylic acid synthase) were upregulated in fruits concomitantly with their downstream metabolites (VSCs ... cysteine synthase), involved in maintaining sulfur levels; the THIK (3-ketoacyl-COA thiolase) family, involved in the ... b) The VSC pathway, which breaks down cysteine and methionine into methanethiol and other VSCs, via the MGL enzyme. (c) ... beginning with transfer of the adenosyl group in ATP to L-methionine by SAM synthase, followed by conversion of L-methionine to ...
... cysteine synthase (CS, E.C. 4.2.99.8). Studies were performed on two weeds, i.e., hemp sesbania [Sesbania exaltata (Raf.) Rybd ... Incorporation of inorganic sulfur into cysteine via cysteine synthase (CS, E.C. 4.2.99.8) (also called O-acetylserine ... Cysteine Synthase Is Essential for Plant Growth and Can Be Used for the Identification of Herbicidal Inhibitors of Cysteine ... Since cysteine synthase was inhibitedin vivo and in vitro by AOA and Boc-AOA, and because there was no major difference in ...
View more ,The class II PHA (polyhydroxyalkanoate) synthases [PHAMCL synthases (medium-chain-length PHA synthases)] are mainly ... The class II PHA (polyhydroxyalkanoate) synthases [PHAMCL synthases (medium-chain-length PHA synthases)] are mainly found in ... Replacement of the catalytic nucleophile cysteine-296 by serine in class II polyhydroxyalkanoate synthase from Pseudomonas ... In contrast to class I and III PHA synthases, the replacement of His-480, which aligns with the conserved base catalyst of the ...
... cysteine synthase,br/,transit peptide,br/,transgenic plants,br/,processing,br/,acetylserine thiol lyase,br/,transgenic tobacco, ... Molecular cloning and expression analyses of mitochondrial and plastidic isoforms of cysteine synthase (O-acetylserine(thiol) ... Molecular cloning and expression analyses of mitochondrial and plastidic isoforms of cysteine synthase (O-acetylserine(thiol) ... Free keywords: amino acids targeting mitochondria chloroplasts cysteine synthase transit peptide transgenic plants processing ...
Cysteine Synthase * MET17 protein, S cerevisiae * O-acetylhomoserine (thiol)-lyase * Carbon-Oxygen Lyases ...
... biochemical studies of serine acetyltransferase reveal why the parasite Entamoeba histolytica cannot form a cysteine synthase ...
Role of a cysteine synthase in Staphylococcus aureus.. Lithgow JK, Hayhurst EJ, Cohen G, Aharonowitz Y, Foster SJ. ...
Ramamurthy V, Swann SL, Spedaliere CJ, Mueller EG (October 1999). "Role of cysteine residues in pseudouridine synthases of ... TRNA pseudouridine32 synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic ... TRNA+pseudouridine32+synthase at the US National Library of Medicine Medical Subject Headings (MeSH) Biology portal. ... Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J (June 1995). "A dual-specificity pseudouridine synthase: an Escherichia ...
... γ-glutamyl-cysteine synthase), which are involved in glutathione reduction and biosynthesis, respectively, in 9 day old cpr1-4 ... γ-glutamyl-cysteine synthase; ETI, effector triggered immunity; GC-MS, gas chromatography coupled to mass spectrometry; GR, ... Baier, M., Noctor, G., Foyer, C. H., and Dietz, K. J. (2000). Antisense suppression of 2-cysteine peroxiredoxin in Arabidopsis ... 2013). Isochorismate synthase 1 is required for thylakoid organization, optimal plastoquinone redox status, and state ...
cysM; cysteine synthase B (O-acetylserine sulfhydrolase B) [KO:K12339] [EC:2.5.1.47] ...
cysteine synthase [KO:K01738] [EC:2.5.1.47]. EUBELI_00752 cysteine synthase [KO:K01738] [EC:2.5.1.47] ...
Mitochondrial cysteine synthase complex regulates O-acetylserine biosynthesis in plants.. Wirtz M, Beard KF, Lee CP, Boltz A, ...
Role of cysteine residues in pseudouridine synthases of different families.. Biochemistry 38 13106-11 1999 ... Pseudouridine synthase TruD, which has a natural circular permutation in the catalytic domain, as well as an insertion of a ... Pseudouridine synthases catalyse the isomerisation of uridine to pseudouridine (Psi) in a variety of RNA molecules, and may ... Pseudouridine synthase RsuA (ribosomal small subunit) and RluC/RluD (ribosomal large subunits), both of which contain an ...
  • In enzymology, a cysteine synthase (EC 2.5.1.47) is an enzyme that catalyzes the chemical reaction O3-acetyl-L-serine + hydrogen sulfide ⇌ {\displaystyle \rightleftharpoons } L-cysteine + acetate Thus, the two substrates of this enzyme are O3-acetyl-L-serine and hydrogen sulfide, whereas its two products are L-cysteine and acetate. (wikipedia.org)
  • This enzyme participates in 3 metabolic pathways: cysteine metabolism, selenoamino acid metabolism, and sulfur metabolism. (wikipedia.org)
  • Overall, the above findings provide evidence that OsCS1 is a potentially important enzyme responsible for the synthesis of cysteine, which could provide a new information about the metabolism of H 2 S in plants. (springer.com)
  • enzyme ) An enzyme that catalyses the biosynthesis of cysteine in microorganisms and plants from o-acetyl-l-serine and hydrogen sulfide . (biologyonline.com)
  • A crystallographic and biochemical study of L. major cysteine synthase, which is a pyridoxyl phosphate-dependent enzyme, is reported. (iucr.org)
  • We examined AOA and its analog, N-t-butoxycarbonyl-AOA (Boc-AOA) for phytotoxicity, interactions with weed pathogens (bioherbicides), and effects on an important pyridoxal requiring enzyme, cysteine synthase (CS, E.C. 4.2.99.8). (scirp.org)
  • In contrast to class I and III PHA synthases, the replacement of His-480, which aligns with the conserved base catalyst of the α/β-hydrolases, with Gln did not affect in vivo enzyme activity and only slightly in vitro enzyme activity. (edu.au)
  • The second conserved histidine His-453 was then replaced by Gln, and the modified enzyme showed only 24% of wild-type in vivo activity, which indicated that His-453 might functionally replace His-480 in class II PHA synthases. (edu.au)
  • TRNA pseudouridine32 synthase (EC 5.4.99.28, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine32 uracil mutase. (wikipedia.org)
  • Cystathionine-β-synthase , also known as CBS , is an enzyme ( EC 4.2.1.22 ) that in humans is encoded by the CBS gene . (wikidoc.org)
  • The human enzyme cystathionine β-synthase is a tetramer and comprises 551 amino acids with a subunit molecular weight of 61 kDa. (wikidoc.org)
  • However, the Anopheles gambiae sequence has a longer N-terminal extension than the human enzyme and contains the conserved histidine and cysteine heme ligand residues like the human heme . (wikidoc.org)
  • Patients with homocystinuria lack the enzyme cystathionine β-synthase (CBS), and many of these individuals do not respond to current treatment protocols. (jci.org)
  • When the term homocystinuria is used, it normally refers to classical homocystinuria caused by deficiency of the enzyme cystathionine beta synthase (CBS). (medindia.net)
  • The enzyme cystathionine beta synthase (CBS) is necessary for conversion of homocysteine to cysteine. (medindia.net)
  • The CdiA-CT(EC536) deployed by uropathogenic Escherichia coli 536 (EC536) is a bacterial toxin 28 (Ntox28) domain that only exhibits ribonuclease activity when bound to the cysteine biosynthetic enzyme O-acetylserine sulfhydrylase A (CysK). (rcsb.org)
  • Steady‐state PC synthesis by eukaryotic PC synthases is by a ping‐pong (substituted enzyme) mechanism. (els.net)
  • The final step of cysteine biosynthesis is catalyzed by the O-acetylserine(thiol)lyase enzyme (OASTL, E.C. 4.2.99). (unboundmedicine.com)
  • The gene ontology annotations also revealed that all sorghum OASTL genes have KOG1252 (Cystathionine beta-synthase and related enzyme) and K01738 (cysteine synthase A) activities. (unboundmedicine.com)
  • Unlike several other corals, Acropora digitifera was found to be lacking cystathionine ß-synthase, an enzyme essential for cysteine biosynthesis. (oist.jp)
  • The enzyme participates in an alternative pathway for L-cysteine biosynthesis that involves a protein-bound thiocarboxylate as the sulfide donor. (expasy.org)
  • Below are the list of possible Bifunctional cystathionine gamma-lyase/cysteine synthase products. (mybiosource.com)
  • Also known as Bifunctional cystathionine gamma-lyase/cysteine synthase (Beta-substituted Ala synthase 4;3) (ARAth-Bsas4;3) (L-cysteine desulfhydrase 1) (DES1) (O-acetylserine (thiol)-lyase) (OASTL) (Protein CS-LIKE). (mybiosource.com)
  • Cystathionine beta synthase catalyzes the upper reaction and cystathionine gamma-lyase catalyzes the lower reaction. (wikidoc.org)
  • H2S is produced from cysteine, involving the enzymes cystathionine beta-synthase and cystathionine gamma-lyase (CSE). (uzh.ch)
  • The catalyzing enzymes serine acetyltransferase (SAT) and O -acetylserine (OAS) thiol lyase (OAS-TL) reversibly form the heterooligomeric Cys synthase complex (CSC). (plantcell.org)
  • Overall, these data suggested that in class II PHA synthases the conserved histidine which was found as general base catalyst in the catalytic triad of enzymes related to the α/β-hydrolase superfamily, was functionally replaced by His-453 which is conserved among all PHA synthases. (edu.au)
  • Genomic and functional characterization of the oas gene family encoding O-acetylserine (thiol) lyases , enzymes catalyzing the final step in cysteine biosynthesis in Arabidopsis thaliana. (thefreedictionary.com)
  • Suh, Dae-Yeon 2009-10-31 00:00:00 Enzymes of the chalcone synthase (CHS) superfamily catalyze the production of a variety of secondary metabolites in bacteria, fungi and plants. (deepdyve.com)
  • H 2 S production has been attributed to two key enzymes in the cysteine biosynthesis pathway, cystathionine β-synthase (CBS) and cystathionine γ-lyase (CGL). (pnas.org)
  • We cloned and characterised genes coding for a cystathionine β-synthase (CβS) and cysteine synthase (CS), key enzymes of the transsulfuration and assimilatory pathways, respectively, from the hemoflagellate protozoan parasite Trypanosoma rangeli . (biomedcentral.com)
  • These reactions are catalysed by two enzymes, CβS (cystathionine β-synthase), which synthesizes cystathionine from homocysteine and serine, and CGL (cystathionine γ-lyase), which forms cysteine from cystathionine [ 6 ]. (biomedcentral.com)
  • Because T. rangeli is required for a response to a variety of oxidative stresses in both mammalian and invertebrate hosts, the present study characterised genes encoding key enzymes of cysteine biosynthesis, a crucial precursor of trypanothione. (biomedcentral.com)
  • Phytochelatin synthases (PC synthases) are soluble enzymes that catalyse the synthesis of heavy metal‐binding peptides termed phytochelatins (PCs) from the tripeptide glutathione (GSH) and related peptides. (els.net)
  • The full extent and mechanistic basis of the roles played by PC synthases as multitasking enzymes remain to be determined, but what is apparent is that the combined protease and peptide polymerase activities of the eukaryotic enzymes versus the limited protease activities of their bacterial counterparts confer a metabolic versatility on the former that the latter lack. (els.net)
  • The β -substituted alanine (Ala) synthase (Bsas) family in the large superfamily of pyridoxal 5′-phosphate-dependent enzymes comprises cysteine (Cys) synthase (CSase) [ O -acetyl-serine (thiol) lyase] and β -cyano-Ala synthase (CASase) in plants. (plantphysiol.org)
  • Structural and Functional studies of crucial pathway enzymes / proteins from E. histolytica & H. pylori i) Cysteine Biosynthetic pathway, sulfur metabolism in E. histolytica ii) Crucial signaling and cytoskeletal proteins involved in Phagocytic cup formation in E. histolytica iii) Replication initiation proteins from H. pylori . (jnu.ac.in)
  • Cysteine synthesis is catalyzed by serine acetyltransferase (SAT) and O-acetylserine (thiol) lyase (OAS-TL) in the cytosol, plastids, and mitochondria of plants. (mendeley.com)
  • However, the relevance of CSC formation in each compartment for flux control of cysteine synthesis remains controversial. (mendeley.com)
  • Here, we demonstrate the interaction between mitochondrial SAT3 and OAS-TL C in planta by FRET and establish the role of the mitochondrial CSC in the regulation of cysteine synthesis. (mendeley.com)
  • Prior research by the Laboratory has identified a series of selected genes, including two key genes for the synthesis of amino acids, cysteine synthase and chorismate mutase. (umsystem.edu)
  • Surprisingly, the biochemical characterization of the recombinant OsLCD2 protein suggests that this protein predominantly possesses cysteine synthesis activity rather than the degradation of cysteine to produce H 2 S, and thus, the gene has been renamed OsCS1 . (springer.com)
  • Burandt P, Schmidt A, Papenbrock J (2001) Cysteine synthesis and cysteine desulfuration in Arabidopsis plants at different developmental stages and light conditions. (springer.com)
  • The class II PHA (polyhydroxyalkanoate) synthases [PHAMCL synthases (medium-chain-length PHA synthases)] are mainly found in pseudomonads and catalyse synthesis of PHAMCLs using CoA thioesters of medium-chain-length 3-hydroxyfatty acids (C6-C14) as a substrate. (edu.au)
  • Attention is also given to the synthesis of glutathione and phytochelatins from cysteine and to the production of DMSP from methionine. (springer.com)
  • Enzymatic synthesis of two isoxazolylalanine isomers by cysteine synthases in Lathyrus species. (ugent.be)
  • Cysteine metabolism. (wikidoc.org)
  • Sulfur and Cysteine Metabolism. (mpg.de)
  • Cysteine (Cys) is the first identified molecule in plant metabolism which includes both sulfur and nitrogen. (unboundmedicine.com)
  • Serine acetyltransferase (SAT) is assumed to consist of a dimer of homotrimers that only forms O -acetylserine (OAS) from Ser and acetyl-CoA with highest efficiency if it is associated with two homodimers of O -acetylserine (thiol) lyase (OAS-TL) in the Cys synthase complex (CSC). (plantcell.org)
  • Álvarez C, Calo L, Romero LC, García I, Gotor C (2010) An O-acetytlserine(thiol)lyase homolog with l -cysteine desulfhydrase activity regulates cysteine homeostasis in Arabidopsis. (springer.com)
  • Transsulfuration, catalyzed by CBS, converts homocysteine to cystathionine , which cystathione gamma lyase converts to cysteine . (wikidoc.org)
  • Cysteine synthase(CSase)(O-acetylserine(thiol)-lyase, EC 4.2.99.8)catalyzes the formation of L-cysteine, the key step in sulfur assimilation in plants, from O-acetyl-L-serine and hydrogen sulfide. (nii.ac.jp)
  • When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the formation of a CymR-CysK complex and transcriptional repression of the CymR regulon occurs. (uniprot.org)
  • In the absence of cysteine, the OAS pool is high and the CymR-CysK complex is mostly dissociated, leading to a faster dissociation of CymR from its DNA targets and the lifting of CymR-dependent repression. (uniprot.org)
  • Remarkably, E. coli serine O-acetyltransferase uses a similar Gly-Asp-Gly-Ile motif to form the "cysteine synthase" complex with CysK. (rcsb.org)
  • Probably unable to interact with SAT and to form the decameric Cys synthase complex (CSC) and is therefore not an enzymatically true OASTL protein. (mybiosource.com)
  • All these cysteine residues identified were found to be located on the surface of the protein according to the available X-ray structure of the oxygenase domain of eNOS. (elsevier.com)
  • The C-terminal domain of cystathionine β-synthase regulates its activity via both intrasteric and allosteric effects and is important for maintaining the tetrameric state of the protein. (wikidoc.org)
  • S is present in numerous pivotal structural and functional compounds such as the amino acids cysteine and methionine, non-protein thiols (glutathione), sulpholipids, vitamins and cofactors, cell wall constituents. (springer.com)
  • The cysteine synthase complex is found throughout bacteria, protozoa, and plants, indicating that CdiA-CT(EC536) exploits a highly conserved protein-protein interaction to promote its toxicity. (rcsb.org)
  • Cysteine, a sulfur-containing amino acid, plays an important role in a variety of cellular functions such as protein biosynthesis, methylation, and polyamine and glutathione syntheses. (biomedcentral.com)
  • The protein kinase B (AKT)/glycogen synthase kinase (GSK) pathways were analyzed using western blot analysis in HepG2 cells and primary mouse hepatocytes treated with high glucose and/or EGCG. (spandidos-publications.com)
  • CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase. (expasy.org)
  • S-nitrosylation, or the replacement of the hydrogen atom in the thiol group of cysteine residues by a -NO moiety, is a physiologically important posttranslational modification. (elsevier.com)
  • However, human eNOS contains 28 other cysteine residues whose potential to undergo S-nitrosylation has not been determined. (elsevier.com)
  • Thus, the goal of this study was to identify the cysteine residues within eNOS that are susceptible to S-nitrosylation in vitro. (elsevier.com)
  • Our data indicate that multiple cysteine residues are capable of undergoing S-nitrosylation in the presence of an excess of a nitrosylating agent. (elsevier.com)
  • In addition, cysteine residues within the reductase domain were identified as undergoing S-nitrosylation. (elsevier.com)
  • D. melanogaster and D. discoides have truncated N-terminal extensions and therefore prevent the conserved histidine and cysteine heme ligand residues . (wikidoc.org)
  • The N-terminal region of phytochelatin synthase contains the active site, as well as four highly conserved cysteine residues that appear to play an important role in heavy-metal-induced phytochelatin catalysis. (ebi.ac.uk)
  • The C-terminal region is rich in cysteines, and may act as a metal sensor, whereby the Cys residues bind cadmium ions to bring them into closer proximity and transferring them to the activation site in the N-terminal catalytic domain [ PMID: 18270423 ]. (ebi.ac.uk)
  • These residues, under oxidative stress, are prone to oxidation in sulfenic acid, which efficiently reacts with GSH leading to a glutathionylated-cysteine derivative (GSSR). (frontiersin.org)
  • Differential expression of two genes for sucrose-phosphate synthase in sugarcane:molecular cloning of the cDNAs and comparative analysis of gene expression'Plant Cell Physiol. (nii.ac.jp)
  • A Mutator insertion was isolated in the cysteine synthase gene. (umsystem.edu)
  • MGL and the ethylene-related gene ACS (aminocyclopropane-1-carboxylic acid synthase) were upregulated in fruits concomitantly with their downstream metabolites (VSCs and ethylene), suggesting a potential association between ethylene biosynthesis and methionine regeneration via the Yang cycle. (nature.com)
  • Animal models of mild hyperhomocysteinemia, induced by vitamin deficiency, 13 heterozygous disruption of the cystathionine β-synthase (CBS) gene, 14 or the combination of both, 15 show an impairment of endothelium-dependent vasoreactivity and regulation of blood flow. (ahajournals.org)
  • The results exclusively showed that OASTL A1-2 gene was 1.12 fold up-regulated in roots, whereas cysteine synthase 26 was 2.25 fold down-regulated in leaves. (unboundmedicine.com)
  • Bonner ER, Cahoon RE, Knapke SM, Jez JM (2005) Molecular basis of cysteine biosynthesis in plants: structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana . (springer.com)
  • 1. S Dharavath, I Raj, S Gourinath (2017) Structure-based mutational studies of O-Acetylserine Sulfhydrylase reveal the reason for the loss of cysteine synthase complex formation in Brucella abortus. (jnu.ac.in)
  • Bertrand EM, Moran DM, McIlvin MR, Hoffman JM, Allen AE, Saito MA (2013) Methionine synthase interreplacement in diatom cultures and communities: implications for the persistence of B 12 use by eukaryotic phytoplankton. (springer.com)
  • In Arabidopsis thaliana , DES1 is the only identified l -Cysteine desulfhydrase located in the cytosol, and it is involved in the degradation of cysteine and the concomitant production of H 2 S in this cell compartment. (plantcell.org)
  • Molecular-Cloning of a Cysteine Synthase Cdna from Arabidopsis-Thaliana. (mpg.de)
  • We report here isolation and characterization of cDNA clones encoding cysteine synthase from spinach(Spinacia oleracea L.). Internal peptide sequences were obtained from V8 protease-digested fragments of purified CSase. (nii.ac.jp)
  • In this article, you will discover the latest research on N-acetyl cysteine (NAC), a readily available, inexpensive amino-acid derivative with four decades of scientific validation. (lifeextension.com)
  • Long relegated to infrequent use in unusual circumstances, the amino acid-derived compound N-acetyl cysteine (NAC) has drawn increased scientific attention. (lifeextension.com)
  • NAC is a slightly modified version of the sulfur-containing amino acid cysteine. (lifeextension.com)
  • L-cysteine, a sulfur-containing amino acid, is indispensable for the survival of virtually all living organisms, from bacteria to higher eukaryotes. (biomedcentral.com)
  • Eukaryotic PC synthases are dimeric proteins made up of two identical 50-55 kDa subunits, each of which consists of a sequence‐conserved 220-240 amino acid residue N ‐terminal domain and a sequence‐variable 200-270 amino acid residue C ‐terminal domain. (els.net)
  • To elucidate the role of intracellular redox status on homocysteine-induced endothelial dysfunction and oxidant stress, heterozygous cystathionine β-synthase-deficient (CBS −/+ ) and wild-type (CBS +/+ ) mice were treated with the cysteine donor l -2-oxothiazolidine-4-carboxylic acid (OTC). (ahajournals.org)
  • Pseudouridine synthases catalyse the isomerisation of uridine to pseudouridine (Psi) in a variety of RNA molecules, and may function as RNA chaperones. (ebi.ac.uk)
  • There are four distinct families of pseudouridine synthases that share no global sequence similarity, but which do share the same fold of their catalytic domain(s) and uracil-binding site and are descended from a common molecular ancestor. (ebi.ac.uk)
  • The catalytic domain consists of two subdomains, each of which has an alpha+beta structure that has some similarity to the ferredoxin-like fold (note: some pseudouridine synthases contain additional domains). (ebi.ac.uk)
  • The de novo pathway is also catalysed by two steps starting with serine acetyltransferase (SAT) to form O-acetylserine (OAS) from L-serine and acetyl-coenzyme A. Subsequently, OAS reacts with sulfide to produce cysteine in an alanyl-transfer reaction by cysteine synthase (CS) [ 7 ]. (biomedcentral.com)
  • The PC synthetic reaction catalysed by eukaryotic PC synthases is a γEC dipeptidyl transferase reaction consisting of two main phases: the initial cleavage (deglycination) of GSH or related γEC‐containing peptides to yield γEC followed by transfer of the γEC unit to the N ‐terminus of another GSH molecule or a preexistent PC. (els.net)
  • Isolation of cDNAs encoding cytosolic and plastidic cysteine synthase isoforms from Solanum tuberosum. (mpg.de)
  • These results suggest that specific SAT isoforms have a role in increasing cysteine production under conditions of heavy-metal stress when increased biosynthesis of glutathione and phytochelatins is required for detoxification purposes. (deepdyve.com)
  • By analogy with the catalytic mechanism of thymidylate synthase ThyA, a conserved cysteine located near the FAD isoalloxazine ring was proposed to act as a nucleophile during catalysis. (archives-ouvertes.fr)
  • Chelation of cadmium ions by phytochelatin synthase: role of the cysteine-rich C-terminal. (ebi.ac.uk)
  • Biochemical analyses of recombinant plant SAT and OAS-TL indicate that the reversible association of the proteins in the cysteine synthase complex (CSC) controls cellular sulfur homeostasis. (mendeley.com)
  • These processes are linked together through 2 inducible proteins that play a major role in the response to oxidative stress: heme oxygenase-1(HO-1) and inducible nitric oxide synthase(INOS). (hackyourgut.com)
  • Expression of a Flax Allene Oxide Synthase Cdna Leads to Increased Endogenous Jasmonic Acid (Ja) Levels in Transgenic Potato Plants but Not to a Corresponding Activation of Ja-Responding Genes. (mpg.de)
  • while among the molecular ones, we highlight squalene synthase, C14α-sterol demethylase, and cysteine proteases. (intechopen.com)
  • Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. (uniprot.org)
  • These findings demonstrate that the RTS pathway is active in T. rangeli , suggesting that this may be the only pathway for cysteine biosynthesis in this parasite. (biomedcentral.com)
  • On the basis of these results, we propose a new model in which the mitochondrial CSC acts as a sensor that regulates the level of SAT activity in response to sulfur supply and cysteine demand. (mendeley.com)
  • Insights into folate/FAD-dependent tRNA methyltransferase mechanism: role of two highly conserved cysteines in catalysis. (archives-ouvertes.fr)
  • Mitochondrial Citrate Synthase from Potato - Predominant Expression in Mature Leaves and Young Flower Buds. (mpg.de)
  • Inhibition of Flower Formation by Antisense Repression of Mitochondrial Citrate Synthase in Transgenic Potato Plants Leads to a Specific Disintegration of the Ovary Tissues of Flowers. (mpg.de)
  • A threading model of the P. aeruginosa PHAMCL synthase PhaC1 was developed based on the homology to the epoxide hydrolase (1ek1) from mouse which belongs to the α/β-hydrolase superfamily. (edu.au)
  • Genome-wide analysis of the chalcone synthase superfamily genes of Physcomitrella patens Koduri, P. (deepdyve.com)
  • By investigating the mechanisms by which animals heritably adapt to P. vranovensis infection, we found that parental infection by P. vranovensis results in increased expression of the cysteine synthases cysl-1 and cysl-2 and the regulator of hypoxia inducible factor rhy-1 in progeny, and that these three genes are required for adaptation to P. vranovensis. (cam.ac.uk)
  • Purification and Characterization of 2-Deoxy-scyllo-inosose Synthase Derived from Bacillus circulans. (go.jp)
  • Structural and biochemical studies of serine acetyltransferase reveal why the parasite Entamoeba histolytica cannot form a cysteine synthase complex. (harvard.edu)
  • Due to the ability of its thiol group to undergo redox reactions, L-cysteine forms the basic building block of all thiol antioxidants, acting as a direct antioxidant and also as a precursor for the biosynthesis of glutathione, trypanothione, or ovothiol [ 2 ]. (biomedcentral.com)
  • Here, we use quantitative molecular-modeling methods to derive a structure of the a - c complex that is not only objectively consistent with the cryo-EM data, but also with correlated mutation analyses of both subunits and with prior cross-linking and cysteine accessibility measurements. (rupress.org)
  • Pseudouridine synthase TruD, which has a natural circular permutation in the catalytic domain, as well as an insertion of a family-specific alpha+beta subdomain. (ebi.ac.uk)
  • The presence of cysteine resulted in reduced OAS export in mitochondria of oastl-C mutants but not in WT mitochondria. (mendeley.com)
  • This is in agreement with the stronger in vitro feedback inhibition of free SAT by cysteine compared with CSC-bound SAT and explains the high OAS export rate of WT mitochondria in the presence of cysteine. (mendeley.com)
  • Involved in maintaining Cys homeostasis through the desulfuration of L-cysteine. (mybiosource.com)
  • AU - Akbudak,M Aydın, AU - Filiz,Ertugrul, AU - Uylas,Senem, Y1 - 2018/11/15/ PY - 2018/07/26/received PY - 2018/11/07/accepted PY - 2018/11/18/pubmed PY - 2019/7/2/medline PY - 2018/11/17/entrez KW - Bioinformatics KW - Cadmium KW - Co-expression KW - Cysteine KW - Sorghum KW - Sulfur SP - 343 EP - 354 JF - Molecular biology reports JO - Mol. (unboundmedicine.com)
  • The structural basis for tRNA recognition and pseudouridine formation by pseudouridine synthase I. (ebi.ac.uk)
  • Pseudouridine synthase RsuA (ribosomal small subunit) and RluC/RluD (ribosomal large subunits), both of which contain an additional N-terminal alpha-L RNA-binding motif. (ebi.ac.uk)
  • Two subunits within the transmembrane domain of the ATP synthase-the c -ring and subunit a -energize the production of 90% of cellular ATP by transducing an electrochemical gradient of H + or Na + into rotational motion. (rupress.org)