Cysteine: A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.Cysteine-Rich Protein 61: A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It may play an important role in the development of branched CAPILLARIES during EMBRYOGENESIS.beta-Keratins: Keratins that form into a beta-pleated sheet structure. They are principle constituents of the corneous material of the carapace and plastron of turtles, the epidermis of snakes and the feathers of birds.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cornified Envelope Proline-Rich Proteins: A family of low molcular-weight proteins that contain PROLINE-RICH PROTEIN DOMAINS. Members of this family play a role in the formation of an insoluble cornified envelope beneath the plasma membrane of stratified squamous epithelial cells.LIM Domain Proteins: A large class of structurally-related proteins that contain one or more LIM zinc finger domains. Many of the proteins in this class are involved in intracellular signaling processes and mediate their effects via LIM domain protein-protein interactions. The name LIM is derived from the first three proteins in which the motif was found: LIN-11, Isl1 and Mec-3.Antigens, Protozoan: Any part or derivative of any protozoan that elicits immunity; malaria (Plasmodium) and trypanosome antigens are presently the most frequently encountered.Malaria, Falciparum: Malaria caused by PLASMODIUM FALCIPARUM. This is the severest form of malaria and is associated with the highest levels of parasites in the blood. This disease is characterized by irregularly recurring febrile paroxysms that in extreme cases occur with acute cerebral, renal, or gastrointestinal manifestations.Protozoan Proteins: Proteins found in any species of protozoan.Plasmodium falciparum: A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics.Cysteine Endopeptidases: ENDOPEPTIDASES which have a cysteine involved in the catalytic process. This group of enzymes is inactivated by CYSTEINE PROTEINASE INHIBITORS such as CYSTATINS and SULFHYDRYL REAGENTS.Cysteine Proteases: A subclass of peptide hydrolases that depend on a CYSTEINE residue for their activity.Cysteine Dioxygenase: An enzyme that catalyzes the conversion of L-CYSTEINE to 3-sulfinoalanine (3-sulfino-L-alanine) in the CYSTEINE metabolism and TAURINE and hypotaurine metabolic pathways.Cysteine Proteinase Inhibitors: Exogenous and endogenous compounds which inhibit CYSTEINE ENDOPEPTIDASES.Cysteine Synthase: An enzyme that catalyzes the biosynthesis of cysteine in microorganisms and plants from O-acetyl-L-serine and hydrogen sulfide. This enzyme was formerly listed as EC 4.2.99.8.Disulfides: Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties.Sulfhydryl Compounds: Compounds containing the -SH radical.Cystine: A covalently linked dimeric nonessential amino acid formed by the oxidation of CYSTEINE. Two molecules of cysteine are joined together by a disulfide bridge to form cystine.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Cathepsins: A group of lysosomal proteinases or endopeptidases found in aqueous extracts of a variety of animal tissues. They function optimally within an acidic pH range. The cathepsins occur as a variety of enzyme subtypes including SERINE PROTEASES; ASPARTIC PROTEINASES; and CYSTEINE PROTEASES.Cystatins: A homologous group of endogenous CYSTEINE PROTEINASE INHIBITORS. The cystatins inhibit most CYSTEINE ENDOPEPTIDASES such as PAPAIN, and other peptidases which have a sulfhydryl group at the active site.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Cathepsin L: A ubiquitously-expressed cysteine protease that plays an enzymatic role in POST-TRANSLATIONAL PROTEIN PROCESSING of proteins within SECRETORY GRANULES.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Papain: A proteolytic enzyme obtained from Carica papaya. It is also the name used for a purified mixture of papain and CHYMOPAPAIN that is used as a topical enzymatic debriding agent. EC 3.4.22.2.Glutathione: A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.Sulfhydryl Reagents: Chemical agents that react with SH groups. This is a chemically diverse group that is used for a variety of purposes. Among these are enzyme inhibition, enzyme reactivation or protection, and labelling.Cathepsin B: A lysosomal cysteine proteinase with a specificity similar to that of PAPAIN. The enzyme is present in a variety of tissues and is important in many physiological and pathological processes. In pathology, cathepsin B has been found to be involved in DEMYELINATION; EMPHYSEMA; RHEUMATOID ARTHRITIS, and NEOPLASM INVASIVENESS.Kinetics: The rate dynamics in chemical or physical systems.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Carbon-Sulfur Lyases: Enzymes that catalyze the cleavage of a carbon-sulfur bond by means other than hydrolysis or oxidation. EC 4.4.Osteonectin: Non-collagenous, calcium-binding glycoprotein of developing bone. It links collagen to mineral in the bone matrix. In the synonym SPARC glycoprotein, the acronym stands for Secreted Protein, Acidic and Rich in Cysteine.Sulfur: An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.Ethylmaleimide: A sulfhydryl reagent that is widely used in experimental biochemical studies.Dithiothreitol: A reagent commonly used in biochemical studies as a protective agent to prevent the oxidation of SH (thiol) groups and for reducing disulphides to dithiols.Serine O-Acetyltransferase: An enzyme that catalyzes the conversion of L-SERINE to COENZYME A and O-acetyl-L-serine, using ACETYL-COA as a donor.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Amino Acid Substitution: The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.Mesylates: Organic salts or esters of methanesulfonic acid.Dithionitrobenzoic Acid: A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate.Sequence Alignment: The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.Structure-Activity Relationship: The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Iodoacetamide: An alkylating sulfhydryl reagent. Its actions are similar to those of iodoacetate.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Reducing Agents: Materials that add an electron to an element or compound, that is, decrease the positiveness of its valence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Protein Structure, Secondary: The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.Methionine: A sulfur-containing essential L-amino acid that is important in many body functions.Bacterial Proteins: Proteins found in any species of bacterium.Sulfinic Acids: Any of the monobasic inorganic or organic acids of sulfur with the general formula RSO(OH). (From McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Iodoacetic Acid: A derivative of ACETIC ACID that contains one IODINE atom attached to its methyl group.Cystathionine gamma-Lyase: A multifunctional pyridoxal phosphate enzyme. In the final step in the biosynthesis of cysteine it catalyzes the cleavage of cystathionine to yield cysteine, ammonia, and 2-ketobutyrate. EC 4.4.1.1.

Activation-dependent adhesion of human platelets to Cyr61 and Fisp12/mouse connective tissue growth factor is mediated through integrin alpha(IIb)beta(3). (1/188)

Cyr61 and connective tissue growth factor (CTGF), members of a newly identified family of extracellular matrix-associated signaling molecules, are found to mediate cell adhesion, promote cell migration and enhance growth factor-induced cell proliferation in vitro, and induce angiogenesis in vivo. We previously showed that vascular endothelial cell adhesion and migration to Cyr61 and Fisp12 (mouse CTGF) are mediated through integrin alpha(v)beta(3). Both Cyr61 and Fisp12/mCTGF are present in normal blood vessel walls, and it has been demonstrated that CTGF is overexpressed in advanced atherosclerotic lesions. In the present study, we examined whether Cyr61 and Fisp12/mCTGF could serve as substrates for platelet adhesion. Agonist (ADP, thrombin, or U46619)-stimulated but not resting platelets adhered to both Cyr61 and Fisp12/mCTGF, and this process was completely inhibited by prostaglandin I(2), which prevents platelet activation. The specificity of Cyr61- and Fisp12/mCTGF-mediated platelet adhesion was demonstrated by specific inhibition of this process with polyclonal anti-Cyr61 and anti-Fisp12/mCTGF antibodies, respectively. The adhesion of ADP-activated platelets to both proteins was divalent cation-dependent and was blocked by RGDS, HHLGGAKQAGDV, or echistatin, but not by RGES. Furthermore, this process was specifically inhibited by the monoclonal antibody AP-2 (anti-alpha(IIb)beta(3)), but not by LM609 (anti-alpha(v)beta(3)), indicating that the interaction is mediated through integrin alpha(IIb)beta(3). In a solid phase binding assay, activated alpha(IIb)beta(3), purified by RGD affinity chromatography, bound to immobilized Cyr61 and Fisp12/mCTGF in a dose-dependent and RGD-inhibitable manner. In contrast, unactivated alpha(IIb)beta(3) failed to bind to either protein. Collectively, these findings identify Cyr61 and Fisp12/mCTGF as two novel activation-dependent adhesive ligands for the integrin alpha(IIb)beta(3) on human platelets, and implicate a functional role for these proteins in hemostasis and thrombosis.  (+info)

Identification of eukaryotic mRNAs that are translated at reduced cap binding complex eIF4F concentrations using a cDNA microarray. (2/188)

Although most eukaryotic mRNAs need a functional cap binding complex eIF4F for efficient 5' end- dependent scanning to initiate translation, picornaviral, hepatitis C viral, and a few cellular RNAs have been shown to be translated by internal ribosome entry, a mechanism that can operate in the presence of low levels of functional eIF4F. To identify cellular mRNAs that can be translated when eIF4F is depleted or in low abundance and that, therefore, may contain internal ribosome entry sites, mRNAs that remained associated with polysomes were isolated from human cells after infection with poliovirus and were identified by using a cDNA microarray. Approximately 200 of the 7000 mRNAs analyzed remained associated with polysomes under these conditions. Among the gene products encoded by these polysome-associated mRNAs were immediate-early transcription factors, kinases, and phosphatases of the mitogen-activated protein kinase pathways and several protooncogenes, including c-myc and Pim-1. In addition, the mRNA encoding Cyr61, a secreted factor that can promote angiogenesis and tumor growth, was selectively mobilized into polysomes when eIF4F concentrations were reduced, although its overall abundance changed only slightly. Subsequent tests confirmed the presence of internal ribosome entry sites in the 5' noncoding regions of both Cyr61 and Pim-1 mRNAs. Overall, this study suggests that diverse mRNAs whose gene products have been implicated in a variety of stress responses, including inflammation, angiogenesis, and the response to serum, can use translational initiation mechanisms that require little or no intact cap binding protein complex eIF4F.  (+info)

Factor VIIa and thrombin induce the expression of Cyr61 and connective tissue growth factor, extracellular matrix signaling proteins that could act as possible downstream mediators in factor VIIa x tissue factor-induced signal transduction. (3/188)

Extracellular interactions of plasma clotting factor VIIa (FVIIa) with tissue factor (TF) on cell surfaces trigger the intracellular signaling events. At present, it is unclear how these signals influence phenotype. To elucidate this, we have used cDNA microarray technology to examine changes in transcriptional program in human fibroblasts in response to exposure to FVIIa. cDNA microarrays revealed that FVIIa binding to TF up-regulated the expression of Cyr61 and CTGF (connective tissue growth factor), the genes that encode extracellular matrix signaling proteins Cyr61 and CTGF, respectively. Northern blot analysis confirmed that FVIIa binding to TF markedly increased the expression of Cyr61 and CTGF in a time- and dose-dependent manner. FVIIa catalytic activity is required for the gene induction. In addition to FVIIa, thrombin also induced the expression of Cyr61 and CTGF. Hirudin abolished the thrombin-induced expression of these mRNAs but not the FVIIa-induced expression. FVIIa-induced expression of Cyr61 appears not to involve the currently known protease-activated receptors (PARs), whereas thrombin-induced expression involves the activation of PAR1 and possibly an additional PAR. Various intracellular signaling pathway inhibitors exhibited different inhibitory pattern on FVIIa and thrombin-induced up-regulation of Cyr61. Cyr61 and CTGF could act as downstream mediators of FVIIa x TF in affecting various biological processes.  (+info)

Adhesion of human skin fibroblasts to Cyr61 is mediated through integrin alpha 6beta 1 and cell surface heparan sulfate proteoglycans. (4/188)

The angiogenic inducer Cyr61 is an extracellular matrix-associated heparin-binding protein that can mediate cell adhesion, stimulate cell migration, and enhance growth factor-stimulated DNA synthesis in both fibroblasts and endothelial cells in culture. In vivo, Cyr61 induces neovascularization and promotes tumor growth. Cyr61 is a prototypic member of a highly conserved family of secreted proteins that includes connective tissue growth factor, nephroblastoma overexpressed, Elm-1/WISP-1, Cop-1/WISP-2, and WISP-3. Encoded by an immediate early gene, Cyr61 synthesis is induced by serum growth factors in cultured fibroblasts and in dermal fibroblasts during cutaneous wound healing. We previously demonstrated that Cyr61 mediates adhesion of vascular endothelial cells and activation-dependent adhesion of blood platelets through direct interaction with integrins alpha(V)beta(3) and alpha(IIb)beta(3), respectively. In this study, we show that the adhesion of primary human skin fibroblasts to Cyr61 is mediated through integrin alpha(6)beta(1) and cell surface heparan sulfate proteoglycans (HSPGs), which most likely serve as co-receptors. Either destruction of cell surface HSPGs or prior occupancy of the Cyr61 heparin-binding site completely blocked cell adhesion to Cyr61. A heparin-binding defective mutant of Cyr61 was unable to mediate fibroblast adhesion through integrin alpha(6)beta(1) but still mediated endothelial cell adhesion through integrin alpha(V)beta(3), indicating that endothelial cell adhesion through integrin alpha(V)beta(3) is independent of the heparin-binding activity of Cyr61. These results identify Cyr61 as a novel adhesive substrate for integrin alpha(6)beta(1) and provide the first demonstration of the requirement for HSPGs in integrin-mediated cell attachment. In addition, these findings suggest that Cyr61 might elicit disparate biological effects in different cell types through interaction with distinct integrin receptors.  (+info)

Muscarinic acetylcholine receptors induce the expression of the immediate early growth regulatory gene CYR61. (5/188)

In brain, muscarinic acetylcholine receptors (mAChRs) modulate neuronal functions including long term potentiation and synaptic plasticity in neuronal circuits that are involved in learning and memory formation. To identify mAChR-inducible genes, we used a differential display approach and found that mAChRs rapidly induced transcription of the immediate early gene CYR61 in HEK 293 cells with a maximum expression after 1 h of receptor stimulation. CYR61 is a member of the emerging CCN gene family that includes CYR61/CEF10, CTGF/FISP-12, and NOV; these encode secretory growth regulatory proteins with distinct functions in cell proliferation, migration, adhesion, and survival. We found that CYR61, CTGF, and NOV were expressed throughout the human central nervous system. Stimulation of mAChRs induced CYR61 expression in primary neurons and rat brain where CYR61 mRNA was detected in cortical layers V and VI and in thalamic nuclei. In contrast, CTGF and NOV expression was not altered by mAChRs neither in neuronal tissue culture nor rat brain. Receptor subtype analyses demonstrated that m1 and m3 mAChR subtypes strongly induced CYR61 expression, whereas m2 and m4 mAChRs had only subtle effects. Increased CYR61 expression was coupled to mAChRs by both protein kinase C and elevations of intracellular Ca(2+). Our results establish that CYR61 expression in mammalian brain is under the control of cholinergic neurotransmission; it may thus be involved in cholinergic regulation of synaptic plasticity.  (+info)

Expression and function of CYR61, an angiogenic factor, in breast cancer cell lines and tumor biopsies. (6/188)

We have previously shown that expression of heregulin (HRG) is closely correlated with breast cancer progression. We have subsequently isolated Cyr61, a ligand for the alpha(v)beta3 integrin that is differentially expressed in HRG-positive cells, and have shown that it is expressed in all of the invasive and metastatic breast cancer cell lines tested. Preliminary evaluation of Cyr61 expression in breast tumor biopsies revealed expression of Cyr61 in about 30% of invasive breast carcinomas. Significantly, we demonstrated that Cyr61 is a downstream effector of HRG action, because a Cyr61-neutralizing antibody abolished the ability of HRG-expressing cells to migrate in vitro. Furthermore, we have shown that HRG-expressing cells denote higher levels of alpha(v)beta3 expression, and we have established that Cyr61 action is mediated, at least in part, through its receptor alpha(v)beta3, because a functional blocking antibody of the alpha(v)beta3 blocked the Matrigel outgrowth of HRG-expressing cells. These results strongly suggest that Cyr61 is necessary for HRG-mediated chemomigration and that Cyr61 plays a functional role in breast cancer progression, possibly through its interactions with the alpha(v)beta3 receptor.  (+info)

The angiogenic factors Cyr61 and connective tissue growth factor induce adhesive signaling in primary human skin fibroblasts. (7/188)

The angiogenic inducers cysteine-rich angiogenic protein 61 (Cyr61) and connective tissue growth factor (CTGF) are structurally related, extracellular matrix-associated heparin-binding proteins. Both can stimulate chemotaxis and promote proliferation in endothelial cells and fibroblasts in culture and induce neovascularization in vivo. Encoded by inducible immediate early genes, Cyr61 and CTGF are synthesized upon growth factor stimulation in cultured fibroblasts and during cutaneous wound healing in dermal fibroblasts. Recently, we have shown that adhesion of primary human fibroblasts to immobilized Cyr61 is mediated through integrin alpha(6)beta(1) and cell surface heparan sulfate proteoglycans (HSPGs) (Chen, N., Chen, C.-C., and Lau, L.F. (2000) J. Biol. Chem. 275, 24953-24961), providing the first demonstration of an absolute requirement for HSPGs in integrin-mediated cell attachment. We show in this study that CTGF also mediates fibroblast adhesion through the same mechanism and demonstrate that fibroblasts adhesion to immobilized Cyr61 or CTGF induces distinct adhesive signaling responses consistent with their biological activities. Compared with fibroblast adhesion to fibronectin, laminin, or type I collagen, cell adhesion to Cyr61 or CTGF induces 1) more extensive and prolonged formation of filopodia and lamellipodia, concomitant with formation of integrin alpha(6)beta(1)-containing focal complexes localized at leading edges of pseudopods; 2) activation of intracellular signaling molecules including focal adhesion kinase, paxillin, and Rac with similar rapid kinetics; 3) sustained activation of p42/p44 MAPKs lasting for at least 9 h; and 4) prolonged gene expression changes including up-regulation of MMP-1 (collagenase-1) and MMP-3 (stromelysin-1) mRNAs and proteins sustained for at least 24 h. Together, these results establish Cyr61 and CTGF as bona fide adhesive substrates with specific signaling capabilities, provide a molecular basis for their activities in fibroblasts through integrin alpha(6)beta(1) and HSPG-mediated signaling during attachment and indicate that these proteins may function in matrix remodeling through the activation of metalloproteinases during angiogenesis and wound healing.  (+info)

CYR61 stimulates human skin fibroblast migration through Integrin alpha vbeta 5 and enhances mitogenesis through integrin alpha vbeta 3, independent of its carboxyl-terminal domain. (8/188)

CYR61, an angiogenic factor and a member of the CCN protein family, is an extracellular matrix-associated, heparin-binding protein that mediates cell adhesion, promotes cell migration, and enhances growth factor-stimulated cell proliferation. CYR61 induces angiogenesis and promotes tumor growth in vivo and is expressed in dermal fibroblasts during cutaneous wound healing. It has been demonstrated recently that adhesion of primary skin fibroblasts to CYR61 is mediated through integrin alpha(6)beta(1) and cell surface heparan sulfate proteoglycans, resulting in adhesive signaling and up-regulation of matrix metalloproteinases 1 and 3. CYR61 is composed of four discrete structural domains that bear sequence similarities to the insulin-like growth factor-binding proteins, von Willebrand factor type C repeat, thrombospondin type 1 repeat, and a carboxyl-terminal (CT) domain that resembles cysteine knots found in some growth factors. In this study, we show that a CYR61 mutant (CYR61DeltaCT) that has the CT domain deleted is unable to support adhesion of primary human skin fibroblasts but is still able to stimulate chemotaxis and enhance basic fibroblast growth factor-induced mitogenesis similar to wild type. In addition, fibroblast migration to CYR61 is mediated through integrin alpha(v)beta(5) but not integrins alpha(6)beta(1) or alpha(v)beta(3). Furthermore, we show that CYR61 binds directly to purified integrin alpha(v)beta(5) in vitro. By contrast, CYR61 enhancement of basic fibroblast growth factor-induced DNA synthesis is mediated through integrin alpha(v)beta(3), a known receptor for CYR61 that mediates CYR61-dependent cell adhesion and chemotaxis in vascular endothelial cells. Thus, CYR61 promotes primary human fibroblast adhesion, migration, and mitogenesis through integrins alpha(6)beta(1), alpha(v)beta(5), and alpha(v)beta(3), respectively. Together, these findings establish CYR61 as a novel ligand for integrin alpha(v)beta(5) and show that CYR61 interacts with distinct integrins to mediate disparate activities in a cell type-specific manner.  (+info)

*CYR61

"The matricellular protein cysteine-rich protein 61 (CCN1/Cyr61) enhances physiological adaptation of retinal vessels and ... "Silencing cAMP-response element binding protein (CREB) identifies cysteine-rich protein 61 (CYR61) as a tumor suppressor gene ... CCN proteins function as matricellular proteins, which are extracellular matrix proteins that play regulatory roles, ... Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded ...

*CCN protein

The CCN protein family includes the following six proteins: CCN1: CYR61 (cysteine-rich angiogenic protein 61) CCN2: CTGF ( ... Members of the CCN protein family are characterized by having four conserved cysteine-rich domains, which include the insulin- ... cysteine-rich angiogenic protein 61 or CCN1), CTGF (connective tissue growth factor or CCN2), and NOV (nephroblastoma ... CCN proteins are a family of extracellular matrix (ECM)-associated proteins involved in intercellular signaling. Due to their ...

*RNF31

... a new class of proteins with a novel cysteine-rich signature". Protein Sci. 8 (7): 1557-61. doi:10.1110/ps.8.7.1557. PMC ... a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein ... RING finger protein 31 is a protein that in humans is encoded by the RNF31 gene. The protein encoded by this gene contains a ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ...

*ARIH2

... a new class of proteins with a novel cysteine-rich signature". Protein Sci. 8 (7): 1557-61. doi:10.1110/ps.8.7.1557. PMC ... Protein ariadne-2 homolog is a protein that in humans is encoded by the ARIH2 gene. GRCm38: Ensembl release 89: ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ...

*RNF216

... a new class of proteins with a novel cysteine-rich signature". Protein Sci. 8 (7): 1557-61. doi:10.1110/ps.8.7.1557. PMC ... TRIAD3 TRIAD3 protein". Chuang, Tsung-Hsien; Ulevitch Richard J (May 2004). "Triad3A, an E3 ubiquitin-protein ligase regulating ... The encoded protein may also function as an E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating ... Chen D, Li X, Zhai Z, Shu HB (2002). "A novel zinc finger protein interacts with receptor-interacting protein (RIP) and ...

*SMCP

Sperm mitochondrial-associated cysteine-rich protein is a protein that in humans is encoded by the SMCP gene. Sperm ... "Entrez Gene: SMCP sperm mitochondria-associated cysteine-rich protein". Behne D, Kyriakopoulos A (2001). "Mammalian selenium- ... and selenium content of the cysteine-rich protein associated with the mitochondrial capsules of mouse sperm". Molecular ... "Sperm mitochondria-associated cysteine-rich protein (SMCP) is an autoantigen in Lewis rats". Biology of Reproduction. 61 (2): ...

*Triflin

... is a cysteine-rich secretory protein, which means it belongs to the CRISP family. This is a group of single chain ... Triflin is a cysteine-rich secretory protein (CRISP), which is excreted by the venom gland of the Habu snake (Trimeresurus ... two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels". Acta Crystallogr F. 61 (Pt ... Other snake venom proteins in the CRISP family: Piscivorin from the Eastern Cottonmouth Latisemin from the Erabu snake Ablomin ...

*WNT1-inducible-signaling pathway protein 3

The CCN acronym is derived from the first three members of the family identified, namely CYR61 (cysteine-rich angiogenic ... WNT1-inducible-signaling pathway protein 3 (WISP3, also named CCN6) is a matricellular protein that in humans is encoded by the ... and a cysteine knot motif within the C-terminal (CT) domain. The CCN family of proteins regulates diverse cellular functions, ... These proteins, together with WISP1 (CCN4), and WISP2 (CCN5) comprise the six-member CCN family in vertebrates. CCN proteins ...

*S100A3

2003). "Characterization of the cysteine-rich calcium-binding S100A3 protein from human hair cuticles". Biochem. Biophys. Res. ... This protein has the highest content of cysteines of all S100 proteins, has a high affinity for Zinc, and is highly expressed ... S100 calcium-binding protein A3 (S100A3) is a protein that in humans is encoded by the S100A3 gene. The protein encoded by this ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038 ...

*NOV (gene)

... cysteine-rich angiogenic inducer 61, or CCN1), CTGF (connective tissue growth factor, or CCN2), and NOV. These proteins, ... The human NOV protein contains 357 amino acids with an N-terminal secretory signal peptide followed by four structurally ... Perbal B, Martinerie C, Sainson R, Werner M, He B, Roizman B (Feb 1999). "The C-terminal domain of the regulatory protein NOVH ... NOV (nephroblastoma overexpressed) also known as CCN3 is a matricellular protein that in humans is encoded by the NOV gene. NOV ...

*Thyroglobulin

... a cysteine-rich module found in proteins from different families". Eur. J. Biochem. 240 (1): 125-33. doi:10.1111/j.1432- ... Thyroglobulin protein accounts for approximately half of the protein content of the thyroid gland. Human TG (HTG) is a ... Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein". J. Biol. ... a short signal peptide may be removed from the N-terminus in the mature protein). The protein is a precursor of the thyroid ...

*Von Willebrand factor type C domain

BMP binding endothelial regulator (BMPER) Cysteine-rich motor neuron 1 protein (CRIM1) Extracellular matrix protein 2 (ECM2) ... Hunt LT, Barker WC (1987). "von Willebrand factor shares a distinctive cysteine-rich domain with thrombospondin and procollagen ... Von Willebrand factor, type C (VWFC or VWC)is a protein domain is found in various blood plasma proteins: complement factors B ... Cysteine-rich angiogenic inducer 61 (CYR61) CCN2: Connective tissue growth factor (CTGF) CCN3: Nephroblastoma overexpressed ( ...

*ADAM23

... cysteine-rich proteins MDC2 and MDC3: novel human cellular disintegrins highly expressed in the brain". The Biochemical Journal ... a novel metalloprotease disintegrin cysteine-rich protein family member expressed by human lymphocytes". The Journal of ... Disintegrin and metalloproteinase domain-containing protein 23 is an enzyme that in humans is encoded by the ADAM23 gene. This ... Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been ...

*CRISP3

Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. GRCh38: ... "Identification of human cysteine-rich secretory protein 3 (CRISP-3) as a matrix protein in a subset of peroxidase-negative ... "Entrez Gene: CRISP3 cysteine-rich secretory protein 3". Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace ... 2004). "Cysteine-rich secretory protein 3 is a ligand of alpha1B-glycoprotein in human plasma". Biochemistry. 43 (40): 12877-86 ...

*ADAM22

... cysteine-rich proteins MDC2 and MDC3: novel human cellular disintegrins highly expressed in the brain". The Biochemical Journal ... specific expression of three novel splice variant forms of human metalloprotease-like disintegrin-like cysteine-rich protein 2 ... Disintegrin and metalloproteinase domain-containing protein 22 also known as ADAM22 is an enzyme that in humans is encoded by ... Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been ...

*C-Raf

... rich in cysteines and stabilized by two zinc ions. It is similar to the diacylglycerol-binding C1 domains of protein kinase C ( ... Li W, Han M, Guan KL (April 2000). "The leucine-rich repeat protein SUR-8 enhances MAP kinase activation and forms a complex ... Mott HR, Carpenter JW, Zhong S, Ghosh S, Bell RM, Campbell SL (August 1996). "The solution structure of the Raf-1 cysteine-rich ... Daub M, Jöckel J, Quack T, Weber CK, Schmitz F, Rapp UR, Wittinghofer A, Block C (November 1998). "The RafC1 cysteine-rich ...

*LDL receptor

... a cysteine-rich protein with multiple Alu sequences in its mRNA". Cell. 39 (1): 27-38. doi:10.1016/0092-8674(84)90188-0. PMID ... A third domain of the protein is rich in O-linked oligosaccharides but appears to show little function. Knockout experiments ... This protein belongs to the LDLR family and is made up of a number of functionally distinct domains, including 3 EGF-like ... e.g. a truncation of the receptor protein at residue number 660 leads to domains 3,4 and 5 of the EGF precursor domain being ...

*LRP6

2002). "Second cysteine-rich domain of Dickkopf-2 activates canonical Wnt signaling pathway via LRP-6 independently of ... Low-density lipoprotein receptor-related protein 6 is a protein that in humans is encoded by the LRP6 gene. LRP6 is a key ... LRP6 acts as a co-receptor with LRP5 and the Frizzled protein family members for transducing signals by Wnt proteins through ... 2001). "LDL-receptor-related protein 6 is a receptor for Dickkopf proteins". Nature. 411 (6835): 321-5. doi:10.1038/35077108. ...

*Resistin

... or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich adipose-derived peptide ... a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family". EMBO J. 19 (15): ... Each protein subunit comprises a carboxy-terminal disulfide-rich beta sandwich "head" domain and an amino-terminal alpha- ... Therefore, the most highly uncovered disulfide bonds found for intact proteins are resistin's disulfides in high-resolution. A ...

*RECK

The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose ... Reversion-inducing-cysteine-rich protein with kazal motifs, also known as RECK, is a human gene, thought to be a metastasis ... 2007). "The reversion-inducing cysteine-rich protein with Kazal motifs (RECK) interacts with membrane type 1 matrix ... RECK reversion-inducing-cysteine-rich protein with kazal motifs". Takahashi C, Sheng Z, Horan TP, et al. (1998). "Regulation of ...

*Kinesin family member 11

"Identification of the protein binding region of S-trityl-L-cysteine, a new potent inhibitor of the mitotic kinesin Eg5". ... Kim ED, Buckley R, Learman S, Richard J, Parke C, Worthylake DK, Wojcik EJ, Walker RA, Kim S (2010). "Allosteric drug ... Kinesin-5 is a molecular motor protein that is essential in mitosis. Kinesin-5 proteins are members of kinesin superfamily, ... The unique assembly of Kinesin-5 proteins not only organizes the protein complex for a different cellular function ( ...

*DMBT1

The DMBT1 protein is a glycoprotein containing multiple scavenger receptor cysteine-rich (SRCR) domains separated by SRCR- ... is the lung scavenger receptor cysteine-rich protein gp-340". J. Biol. Chem. 275 (51): 39860-6. doi:10.1074/jbc.M006928200. ... Deleted in malignant brain tumors 1 protein is a protein that in humans is encoded by the DMBT1 gene. Loss of sequences from ... a member of the scavenger receptor cysteine-rich superfamily". J. Biol. Chem. 277 (35): 32109-15. doi:10.1074/jbc.M203788200. ...

*Caspase 10

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of ... 1997). "A serine/arginine-rich nuclear matrix cyclophilin interacts with the C-terminal domain of RNA polymerase II". Nucleic ... This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene ... Vincenz, C; Dixit V M (March 1997). "Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE ...

*DEFB126

Beta-defensin 126 is a protein that in humans is encoded by the DEFB126 gene. Defensins are cysteine-rich cationic polypeptides ... The protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found ... The encoded protein is highly similar to an epididymal-specific secretory protein (ESP13.2) from cynomolgus monkey. It has been ... Perry AC, Jones R, Moisyadi S, Coadwell J, Hall L (Oct 1999). "The novel epididymal secretory protein ESP13.2 in Macaca ...

*Urease

... they are cysteine-rich enzymes, resulting in the enzyme molar masses between 190 and 300kDa. An exceptional enzyme is the ... Sumner's work was the first demonstration that a pure protein can function as an enzyme, and led eventually to the recognition ... Cysteine residues are common in the flap region of the enzymes, which have been determined not to be essential in catalysis, ... The breakdown of this intermediate is then helped by a sulfhydryl group of a cysteine located near the active site. A hydrogen ...

*Phage display

... is a laboratory technique for the study of protein-protein, protein-peptide, and protein-DNA interactions that ... pIII is composed of three domains (N1, N2 and CT) connected by glycine-rich linkers. The N2 domain binds to the F pilus during ... when using the BamHI site located at position 198 one must be careful of the unpaired Cysteine residue (C201) that could cause ... a gene encoding a protein of interest is inserted into a phage coat protein gene, causing the phage to "display" the protein on ...
A set of immediate-early genes that are rapidly activated by serum or purified platelet-derived growth factor in mouse 3T3 fibroblasts has been previously identified. Among these genes, several are related to known or putative transcription factors and growth factors, supporting the notion that some of these genes encode regulatory molecules important to cell growth. We show here that a member of this set of genes, cyr61 (originally identified by its cDNA 3CH61), encodes a 379-amino-acid polypeptide rich in cysteine residues. cyr61 can be induced through protein kinase C-dependent and -independent pathways. Unlike many immediate-early genes that are transiently expressed, the cyr61 mRNA is accumulated from the G0/G1 transition through mid-G1. This expression pattern is due to persistent transcription, while the mRNA is rapidly turned over during the G0/G1 transition and in mid-G1 at the same rate. In logarithmically growing cells, the cyr61 mRNA level is constant throughout the cell cycle.
Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. CYR61 is a secreted, extracellular matrix (ECM)-associated signaling protein of the CCN family (CCN intercellular signaling protein). CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers. CYR61 was first identified as a protein encoded by a ...
Chao Zhang, Dustin van der Voort, Hong Shi, Rongli Zhang, Yulan Qing, Shuichi Hiraoka, Minoru Takemoto, Koutaro Yokote, Joseph V. Moxon, Paul Norman, Laure Rittié, Helena Kuivaniemi, G. Brandon Atkins, Stanton L. Gerson, Guo-Ping Shi, Jonathan Golledge, Nianguo Dong, Bernard Perbal, Domenick A. Prosdocimo, Zhiyong Lin. ...
Detect and quantitate human cysteine rich angiogenic inducer 61 (CYR61) in serum and cell culture medium using a homogeneous AlphaLISA no-wash assay.
Rabbit Polyclonal Anti-Cyr61/CCN1 Antibody. Validated: WB, Simple Western, B/N, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Rabbit. 100% Guaranteed.
CYR61 is a growth factor-inducible, immediate-early gene that has multifaceted activities in various cancers.CYR61 is a secreted, cysteine-rich, heparin-binding protein.
TY - JOUR. T1 - Interleukin-13 modulates collagen homeostasis in human skin and keloid fibroblasts. AU - Oriente, Alfonso. AU - Fedarko, Neal S.. AU - Pacocha, Sarah E.. AU - Huang, Shau Ku. AU - Lichtenstein, Lawrence M.. AU - Essayan, David M.. PY - 2000/3/1. Y1 - 2000/3/1. N2 - Interleukin (IL)-13 has been implicated in the pathogenesis of various diseases characterized by fibrosis. We describe the effects of IL-13 on collagen homeostasis from normal (NF) and keloid (KF) fibroblasts and compare these effects with those of IL-4 and transforming growth factor (TGF)-β1. Total collagen generation was up-regulated in NF after 48 h of stimulation by IL-13; in KF, IL-13 stimulated a more rapid collagen response. The kinetics and magnitude of collagen generation induced by IL-13 were equivalent to those induced by similar concentrations of IL-4 and TGF-β1. Collagen type I production paralleled total collagen generation from both NF and KF; however, IL-4-induced collagen type I and total collagen ...
Rabbit Polyclonal Anti-Cyr61/CCN1 Antibody [Biotin]. Validated: WB, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Rabbit. 100% Guaranteed.
CRIP1 antibody (cysteine-rich protein 1 (intestinal)) for IP, WB. Anti-CRIP1 pAb (GTX131195) is tested in Human samples. 100% Ab-Assurance.
Hypoxia has a profound influence on progression and metastasis of malignant tumors. In the present report, we used the oligonucleotide microarray technique to identify new hypoxia-inducible genes in malignant melanoma with a special emphasis on angiogenesis factors. A commercially available Affymetrix gene chip system was used to analyze five melanoma cell lines of different aggressiveness. A total of 160 hypoxia-inducible genes were identified, clustering in four different functional clusters. In search of putative angiogenesis and tumor progression factors within these clusters, Cyr61, a recently discovered angiogenesis factor, was identified. Cyr61 was hypoxia-inducible in low aggressive melanoma cells; however, it showed constitutive high expression in highly aggressive melanoma cells. Further analyses of transcriptional mechanisms underlying Cyr61 gene expression under hypoxia demonstrated that an AP-1 binding motif within the Cyr61 promoter plays a central role in the hypoxic regulation of ...
Cysteine-rich protein 61 (Cyr61) is a secreted matrix-associated protein that regulates a broad spectrum of biological and cellular activities. This study aimed to investigate the role of Cyr61 in progressive kidney fibrosis induced by unilateral ureteral obstruction (UUO) surgery in mice. The expression of Cyr61 transcripts and proteins in the obstructed kidneys were increased from day 1 and remained high until day 10 after surgery. Immunohistochemistry indicated that Cyr61 was expressed mainly in renal tubular epithelial cells. The upregulated Cyr61 in UUO kidneys was reduced in mice treated with pan-transforming growth factor-β (TGF-β) antibody. The role of TGF-β in tubular Cyr61 upregulation after obstructive kidney injury was further supported by experiments showing that TGF-β1 stimulated Cyr61 expression in cultured tubular epithelial cells. Notably, the upregulation of Cyr61 in UUO kidneys was followed by a marked increase in monocyte chemoattractant protein 1 (MCP-1) ...
The CCN proteins are key signalling and regulatory molecules involved in many vital biological functions, including cell proliferation, angiogenesis, tumourigenesis and wound healing. How these proteins influence such a range of functions remains incompletely understood but is probably related to their discrete modular nature and a complex array of intra- and inter-molecular interactions with a variety of regulatory proteins and ligands. Although certain aspects of their biology can be attributed to the four individual modules that constitute the CCN proteins, recent results suggest that some of their biological functions require cooperation between modules. Indeed, the modular structure of CCN proteins provides important insight into their structure-function relationships.
Cysteine-rich 61 (Cyr61), a member of the CCN protein family, possesses diverse functionality in cellular processes such as adhesion, migration, proliferation, and survival. Cyr61 can also function as an oncogene or a tumour suppressor, depending on the origin of the cancer. Only a few studies have reported Cyr61 expression in colorectal cancer. In this study, we assessed the Cyr61 expression in 251 colorectal cancers with clinical follow up. We examined Cyr61 expression in 6 colorectal cancer cell lines (HT29, Colo205, Lovo, HCT116, SW480, SW620) and 20 sets of paired normal and colorectal cancer tissues by western blot. To validate the association of Cyr61 expression with clinicopathological parameters, we assessed Cyr61 expression using tissue microarray analysis of primary colorectal cancer by immunohistochemical analysis. We verified that all of the cancer cell lines expressed Cyr61; 2 cell lines (HT29 and Colo205) demonstrated Cyr61 expression to a slight extent, while 4 cell lines (Lovo, HCT116,
Dr. Villa-Diazs research focuses on understanding and elucidating the self-renewal properties of hPSCs, which are influenced by the microenvironment in which they are cultured, in particular the extracellular matrix (ECM). The ECM is a multifunctional regulator of cellular behavior serving functions beyond those as a scaffold for the organization of cells into tissues. ECM proteins modulate the activity and bioavailability of growth factors, cytokines, and chemokines via direct binding and signaling via cell-surface receptors including integrins and matricellular proteins, to regulate cellular functions ...
CYR61/CCN1 Antibody 26689-1-AP has been identified with ELISA, WB, IF. 26689-1-AP detected 42 kDa band in A431 cells with 1:500-1:3000 dilution...
Aged, Aging, Cell, Collagen, Cysteine, Cysteine-rich Protein 61, Extracellular Matrix, Fibroblasts, Homeostasis, Human, Il-1, Interleukin, Interleukin 1, Matrix Metalloproteinase-1, Premature Aging, Production, Report, Skin, Skin Aging, Sun
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Looking for online definition of Nerve growth factor-inducible protein PC4 in the Medical Dictionary? Nerve growth factor-inducible protein PC4 explanation free. What is Nerve growth factor-inducible protein PC4? Meaning of Nerve growth factor-inducible protein PC4 medical term. What does Nerve growth factor-inducible protein PC4 mean?
Purpose: : Proliferative vitreoretinopathy (PVR) is a well known complication of ocular trauma and rhegmatogenous retinal detachment leading to progressive deterioration of vision and potential blindness. Although several cell types and cytokines have been detected in PVR membranes their definite pathogenic mechanisms are still unknown. Members of the CCN family of matricellular proteins are multifunctional growth factors representing a novel class of extracellular matrix-associated signalling molecules. Several studies demonstrated that members of the CCN family are involved in the control of cell adhesion, chemotaxis, cell migration and cell proliferation. In particular, it was demonstrated that CTGF, CYR61 and NOV possess fibrotic and angiogenic activity being involved in wound healing and several fibrotic diseases. The aim of the present study was to investigate whether CCN proteins are expressed and increased in epiretinal membranes to shed more light on the pathogenesis of PVR Methods: : ...
Dr. Steven J. Cyr M.D., F.A.A.O.S Owner of The Orthopaedic & Spine Institute One of my God-given strengths is empathy. When I talk to someone who is hurting especially people who have had failed surgery... Read More... ...
TY - JOUR. T1 - Involvement of cysteine-rich protein 61 in the epidermal growth factor-induced migration of human anaplastic thyroid cancer cells. AU - Chin, Li Han. AU - Hsu, Sung Po. AU - Zhong, Wen-Bin. AU - Liang, Yu Chih. PY - 2016. Y1 - 2016. N2 - Anaplastic thyroid cancer (ATC) is among the most aggressive types of malignant cancer. Epidermal growth factor (EGF) plays a crucial role in the pathogenesis of ATC, and patients with thyroid carcinoma typically exhibit increased cysteine-rich protein 61 (Cyr61). In this study, we found that EGF treatment induced cell migration, stress fiber formation, Cyr61 mRNA and protein expressions, and Cyr61 protein secretion in ATC cells. The recombinant Cyr61 protein significantly induced cell migration; however, inhibition of Cyr61 activity by a Cyr61-specific antibody abrogated EGF-induced cell migration. EGF treatment also affected epithelial-to-mesenchymal transition (EMT)-related marker protein expression, as evidenced by an increase in vimentin and ...
Cysteine-rich proteins (CRPs) encoded by some plant viruses in diverse genera function as RNA silencing suppressors. Within the N-terminal portion of CRPs encoded by furoviruses, there are six conserved cysteine residues and a Cys-Gly-X-X-His motif (
The proper execution of gastrulation requires that cell signalling, cell adhesion and cell migration are all precisely coordinated and integrated. In their investigations into how this might occur, Latinkic et al. studied Cyr61, a CCN-family, secreted, extracellular matrix (ECM)- associated protein. CCN proteins are very versatile - they can mediate cell adhesion and migration, for example, and can induce signalling events - all expected features of candidate gastrulation regulators. The authors now report, on p. 2429, that Cyr61 is an important regulator of gastrulation movements: both its overexpression and inhibition disrupt gastrulation in frog embryos, perhaps because Cyr61 is required for the assembly of a fibronectin-rich ECM and because it regulates cell- cell and cell-matrix adhesion. Intriguingly, Xcyr61 also appears to both stimulate and inhibit Wnt signalling in a context-dependent manner. Future studies into the different domains of Cyr61 should reveal where its many activities ...
The extracellular matrix plays critical roles in regulating diverse biological and pathological processes, including developmental morphogenesis, tissue repair and remodeling, inflammation and host defense, and progression of diseases such as cancer. Our laboratory is interested in the biological functions of CCNs, a family of cysteine-rich, dynamically expressed matricellular proteins that regulates a broad range of cellular activities through direct binding to integrin receptors and heparan sulfate proteoglycans. Ccn1- and Ccn2-null mice are embryonic and perinatal lethal due to cardiovascular and skeletal defects, respectively, underscoring the importance of these matrix proteins. In addition, aberrant expression of Ccn1 and Ccn2 is associated with pathological conditions including wound healing, atherosclerosis, restenosis, rheumatoid arthritis, and cancer.. Our current studies aim to understand how CCN proteins function through integrin-mediated signaling to regulate diverse processes, ...
From NCBI Gene:. This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]. From UniProt: ...
Affiliation (Current):金沢大学,附属病院,助教, Research Field:Radiation science,Basic Section 52040:Radiological sciences-related, Keywords:神経芽腫,内照射療法,内用療法,放射線管理,matricellular protein,annexin V,α線,tenascin C,消化器癌,虚血性心疾患, # of Research Projects:7, # of Research Products:41, Ongoing Project:Development of new therapeutoc strategy for high-risk chemotherapy-refractory neuroblastoma
Pseudoaneurysms result from disruption of all three layers of the arterial wall with the formation of a bloodfilled cavity lined by endothelium and supported by granulation, adventitial and periadventitial tissues [15]. True aortic aneurysms are expansions of the entire vessel wall, caused by segmental weakening of the wall due to a primary or secondary defect in the matrix structures. Loss of elastin has long been considered the hallmark of aneurysm formation, but it is now accepted that impaired collagen homeostasis is the main cause [16]. Aortic aneurysms are usually seen abdominally in older people. Hypertension, smoking and hypercholesterolemia are predisposing factors [17]. Thoracic aneurysms are mainly found in young people however and generally occur due to an inherited connective tissue disorder [17, 18]. Even apart from genetic disorders of collagen and elastin, a familial predisposition has been shown as well [19]. Rupture of the thoracic aorta in Friesian horses is not associated ...
My research focuses on elucidating the mechanism(s) of cell death and survival of primary alveolar epithelial cells and lung fibroblasts in response to the extracellular matrix associated signaling molecule, CCN1/Cyr61. CCN1/Cyr61 is an extracellular matrix (ECM)-associated signaling molecule that functions to promote cell adhesion, migration, survival and differentiation in the context of vascular development. Most recently we have shown that CCN1/Cyr61 can also modulate cell death in certain cells and can promote cell death in response to TNFa. In the context of the lung, I have found that CCN1/Cyr61 together with TNFa causes apoptosis of alveolar epithelial cells and lung fibroblasts. Because CCN1/Cyr61 has been shown to be induced in the lungs of patients with COPD and ARDS, I hypothesize that CCN1/Cyr61, together with inflammatory mediators such as TNFa causes cell death of primary lung cells in vivo, thus contributing to lung injury. I have also found that CCN1/Cyr61 functional knock-out ...
CCN3 antibody (nephroblastoma overexpressed gene) for ICC/IF, IHC-P, WB. Anti-CCN3 pAb (GTX103377) is tested in Human, Mouse samples. 100% Ab-Assurance.
Twisted gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning in Drosophila and Xenopus by regulating…
Complete information for CRIPAK gene (Protein Coding), Cysteine Rich PAK1 Inhibitor, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Periostin has since been demonstrated to be important in heart valve development and myocardial infarction because it promotes collagen fibrogenesis, inhibits differentiation of progenitor cells into cardiomyocytes and is essential in maintaining the biomechanical properties of the adult myocardium. This antibody detects a 93-kDa periostin doublet, which corresponds to the two largest forms of the alternatively spliced region of periostin with a distinctive ...
To examine the function of CRP2 in vivo, we generated Csrp2−/− mice by targeted mutation. The LIM domains of CRP2 mediate interactions with its binding partners including zyxin, α-actinin, and CRP2BP.12,19,30 Thus, we targeted the first LIM domain by disrupting exon 3, the largest coding exon. No message was detected in Csrp2−/− mouse RNA when exon 3 was used as a probe, although a smaller transcript was detected by RT-PCR. Nevertheless, no CRP2 was detected in protein isolated from Csrp2−/− mouse aorta by Western blot analysis using CRP2(91-98) antiserum. Furthermore, immunostaining of aortic sections with CRP2(93-108) antiserum did not detect CRP2 expression in Csrp2−/− mice. We cannot exclude the possibility that a truncated protein could be generated because the two antisera used in this study were against epitopes C-terminal to those encoded by exon 2. However, even if the truncated transcript were translated, it would not encode either LIM domain due to a frame shift. ...
Neurons depend upon neurotransmitter release through regulated exocytosis to accomplish the immense processing performed within the central nervous system. The SNARE hypothesis points to a family of proteins that are thought to enable the membrane fusion that leads to exocytosis. The secondary structure of SNAP-25 is unique among SNARE proteins in that it has two alpha helical SNARE motifs and a cysteine rich (C85, C88, C90, C92) membrane interacting region but notransmembrane domain. The cysteines may be modified by palmitoylation or oxidation but the role of these modifications in vivo is not well understood. Our goal is to elucidate possible regulatory roles of SNAP-25 that relate to its unique structure and these reversible modifications. However, the study of SNAP-25 in reconstituted systems is hampered by a lack of readily available palmitoylated SNAP-25. A method for in vitro palmitoylation of SNAP-25 by HIP14, a neuronal acyltransferase, is described along with the application of a biotinylation
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
The sex-inducer of the spherical green alga Volvox carteri is one of the most potent biological effector molecules known: it is released into the medium by sexual males and triggers the switch to the sexual cleavage program in the reproductive cells of vegetatively grown males and females even at concentrations as low as 10 (-16) M. In an adult Volvox alga, all cells are embedded in an extensive extracellular matrix (ECM), which constitutes >99% of the volume of the spheroid. There exist no cytoplasmic connections between the cells in an adult alga, so any signal transduction between different cells or from the organisms environment to a reproductive cell must involve the ECM. Recently, a small cysteine-rich extracellular protein, VCRP, was identified in Volvox and shown to be quickly synthesized by somatic cells in response to the sex-inducer. Due to its characteristics, VCRP was speculated to be an extracellular second messenger from somatic cells to reproductive cells. Here a related ...
Complete information for RUBCN gene (Protein Coding), RUN And Cysteine Rich Domain Containing Beclin 1 Interacting Protein, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Buy our Recombinant Human CCN1 protein (Fc Chimera). Ab191971 is a full length protein produced in HEK 293 cells and has been validated in SDS-PAGE, HPLC…
Neurons depend upon neurotransmitter release through regulated exocytosis to accomplish the immense processing performed within the central nervous system. The SNARE hypothesis points to a family of proteins that are thought to enable the membrane fusion that leads to exocytosis. The secondary structure of SNAP-25 is unique among SNARE proteins in that it has two alpha helical SNARE motifs and a cysteine rich (C85, C88, C90, C92) membrane interacting region but notransmembrane domain. The cysteines may be modified by palmitoylation or oxidation but the role of these modifications in vivo is not well understood. Our goal is to elucidate possible regulatory roles of SNAP-25 that relate to its unique structure and these reversible modifications. However, the study of SNAP-25 in reconstituted systems is hampered by a lack of readily available palmitoylated SNAP-25. A method for in vitro palmitoylation of SNAP-25 by HIP14, a neuronal acyltransferase, is described along with the application of a ...
How is SWI/SNF Related, Matrix-Associated, Actin-Dependent, Regulator of Chromatin abbreviated? SMARC stands for SWI/SNF Related, Matrix-Associated, Actin-Dependent, Regulator of Chromatin. SMARC is defined as SWI/SNF Related, Matrix-Associated, Actin-Dependent, Regulator of Chromatin somewhat frequently.
Collagens are substantial constituents of the extracellular matrix (ECM) of every organ system, and virtually every cell in a tissue has the opportunity to interact with fibrillar or basement membrane collagens in the context of tissue development, remodeling, or homeostasis. Tissue architecture is governed by the structure imparted by the ECM; maintenance of this architecture is a barrier to malignant transformation. However, documentation that dysfunctional or inappropriate ECM contributes directly to tumor development is uncommon.. Collagen secretion, fibrillogenesis, and deposition are the result of a precise series of biochemical events that are facilitated by extracellular adaptor proteins, including proteogylcans (e.g., fibromodulin, lumican, and decorin) and matricellular proteins such as secreted protein acidic and rich in cysteine (SPARC; ref. 1). Of these extracellular adaptor proteins, SPARC has received considerable attention in the context of tumor development and progression. ...

CYR61 - WikipediaCYR61 - Wikipedia

"The matricellular protein cysteine-rich protein 61 (CCN1/Cyr61) enhances physiological adaptation of retinal vessels and ... "Silencing cAMP-response element binding protein (CREB) identifies cysteine-rich protein 61 (CYR61) as a tumor suppressor gene ... CCN proteins function as matricellular proteins, which are extracellular matrix proteins that play regulatory roles, ... Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded ...
more infohttps://en.wikipedia.org/wiki/CYR61

Cysteine-Rich Protein 61 Plays a Proinflammatory Role in Obstructive Kidney FibrosisCysteine-Rich Protein 61 Plays a Proinflammatory Role in Obstructive Kidney Fibrosis

Cysteine-rich protein 61 (Cyr61) is a secreted matrix-associated protein that regulates a broad spectrum of biological and ... Home » Cysteine-Rich Protein 61 Plays a Proinflammatory Role in Obstructive Kidney Fibrosis ... Cysteine-Rich Protein 61 Plays a Proinflammatory Role in Obstructive Kidney Fibrosis. ... The expression of Cyr61 transcripts and proteins in the obstructed kidneys were increased from day 1 and remained high until ...
more infohttp://connection.ebscohost.com/c/articles/87624650/cysteine-rich-protein-61-plays-proinflammatory-role-obstructive-kidney-fibrosis

Combined Treatment with Troglitazone and Lovastatin Inhibited Epidermal Growth Factor-Induced Migration through the...Combined Treatment with Troglitazone and Lovastatin Inhibited Epidermal Growth Factor-Induced Migration through the...

... and significantly inhibited EGF-induced Cyr61 mRNA and protein expression as well as Cyr61 secretion. Moreover, the ... the cAMP response element-binding protein (CREB) and extracellular signal-regulated kinase (ERK), were decreased in cells ... can induce cell migration through the induction of cysteine-rich protein 61 (Cyr61) in human anaplastic thyroid cancer (ATC) ... and Lovastatin Inhibited Epidermal Growth Factor-Induced Migration through the Downregulation of Cysteine-Rich Protein 61 in ...
more infohttps://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0118674

Cysteine-rich heparin-binding protein 61 | definition of cysteine-rich heparin-binding protein 61 by Medical dictionaryCysteine-rich heparin-binding protein 61 | definition of cysteine-rich heparin-binding protein 61 by Medical dictionary

What is cysteine-rich heparin-binding protein 61? Meaning of cysteine-rich heparin-binding protein 61 medical term. What does ... Looking for online definition of cysteine-rich heparin-binding protein 61 in the Medical Dictionary? cysteine-rich heparin- ... cysteine-rich domain associated with ring and TRAF domain. *Cysteine-rich domain associated with RING and Traf domains protein ... redirected from cysteine-rich heparin-binding protein 61) CYR61. A gene on chromosome 1p22.3 that encodes a growth factor- ...
more infohttps://medical-dictionary.thefreedictionary.com/cysteine-rich+heparin-binding+protein+61

Cyr 61 elisa kit | Rat CYR61 Cysteine-rich protein 61 (Cyr 61) ELISA KitCyr 61 elisa kit | Rat CYR61 Cysteine-rich protein 61 (Cyr 61) ELISA Kit

Rat CYR61 Cysteine-rich protein 61 (Cyr 61) ELISA Kit (MBS9314809) product datasheet at MyBioSource, ELISA Kits ... CYR61 Cysteine-rich protein 61 (Cyr 61), ELISA Kit. ★Popular Item★ Also Known As Rat CYR61 Cysteine-rich protein 61 (Cyr 61) ... Avidin (AVD) Protein. • Insulin-1 (Ins1) Recombinant Protein. • SNAI2 cDNA Clone. • Histone-Binding Protein RBBP4 (RBBP4) ELISA ... Kit for analyzing the presence of the CYR61 Cysteine-rich protein 61 (Cyr 61) ELISA Kit target analytes in biological samples. ...
more infohttps://www.mybiosource.com/prods/ELISA-Kit/Rat/CYR61-Cysteine-rich-protein-61-Cyr-61/Cyr-61/datasheet.php?products_id=9314809

Involvement of cysteine-rich protein 61 in the epidermal growth factor-induced migration of human anaplastic thyroid cancer...Involvement of cysteine-rich protein 61 in the epidermal growth factor-induced migration of human anaplastic thyroid cancer...

Chin, L. H., Hsu, S. P., Zhong, W-B., & Liang, Y. C. (2016). Involvement of cysteine-rich protein 61 in the epidermal growth ... Chin, LH, Hsu, SP, Zhong, W-B & Liang, YC 2016, Involvement of cysteine-rich protein 61 in the epidermal growth factor-induced ... Involvement of cysteine-rich protein 61 in the epidermal growth factor-induced migration of human anaplastic thyroid cancer ... Involvement of cysteine-rich protein 61 in the epidermal growth factor-induced migration of human anaplastic thyroid cancer ...
more infohttps://tmu.pure.elsevier.com/en/publications/involvement-of-cysteine-rich-protein-61-in-the-epidermal-growth-f

Porcine Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit Manufacturers in DelhiPorcine Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit Manufacturers in Delhi

Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit OSCAR DIAGNOSTIC SERVICES PVT. LTD.is an India based Company in Delhi. ... Porcine Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit. *Porcine GDF7 (Growth Differentiation Factor 7) ELISA ... Porcine Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit » Porcine Cyr61 (Cysteine Rich Protein, Angiogenic ... Porcine Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit. Porcine Cyr61 (Cysteine Rich Protein, Angiogenic ...
more infohttps://www.odspl.com/sub-product/Porcine+Cyr61+%28Cysteine+Rich+Protein%2C+Angiogenic+Inducer+61%29+ELISA+Kit.php

Ezrin regulates skin fibroblast size/mechanical properties and YAP-dependent proliferation | SpringerLinkEzrin regulates skin fibroblast size/mechanical properties and YAP-dependent proliferation | SpringerLink

Quan T, Qin Z, Voorhees JJ, Fisher GJ (2012) Cysteine-rich protein 61 (CCN1) mediates replicative senescence-associated ... CCN1 and CCN2 protein levels were determined by Western blots. Protein levels were normalized by β-actin as a loading control. ... Clucas J, Valderrama F (2014) ERM proteins in cancer progression. J Cell Sci 127:267-275CrossRefPubMedGoogle Scholar ... Hsu YY, Shi GY, Kuo CH, Liu SL, Wu CM, Ma CY, Lin FY, Yang HY, Wu HL (2012) Thrombomodulin is an ezrin-interacting protein that ...
more infohttps://link.springer.com/article/10.1007%2Fs12079-017-0406-6

GO Gene ListGO Gene List

Cartilage oligomeric matrix protein. NM_016685. Gene Info. Crispld2. Cysteine-rich secretory protein LCCL domain containing 2. ... Cysteine rich protein 61. NM_010516. Gene Info. Dhtkd1. Dehydrogenase E1 and transketolase domain containing 1. NM_001081131. ... Amyloid beta (A4) precursor-like protein 1. NM_007467. Gene Info. Aplp2. Amyloid beta (A4) precursor-like protein 2. NM_ ... Low density lipoprotein receptor-related protein associated protein 1. NM_013587. Gene Info. ...
more infohttps://cgap.nci.nih.gov/Genes/GoGeneQuery?PAGE=1&ORG=Mm&GOID=1901681

GO Gene ListGO Gene List

Cysteine rich protein 61. NM_010516. Gene Info. Dab2. Disabled 2, mitogen-responsive phosphoprotein. NM_023118. NM_001037905. ... Rho GTPase activating protein 6. NM_009707. NM_178754. Gene Info. Arpc2. Actin related protein 2/3 complex, subunit 2. NM_ ... Dentin matrix protein 1. NM_016779. Gene Info. Ecm2. Extracellular matrix protein 2, female organ and adipocyte specific. NM_ ... Adaptor-related protein complex 1 associated regulatory protein. NM_145964. Gene Info. ...
more infohttps://cgap.nci.nih.gov/Genes/GoGeneQuery?PAGE=1&ORG=Mm&GOID=0010810

Dr. Susheela  Tridandapani - Cancer Researcher at OSUCCC - JamesDr. Susheela Tridandapani - Cancer Researcher at OSUCCC - James

Role of cysteine-rich 61 protein (CCN1) in macrophage-mediated oncolytic herpes simplex virus clearance.. Thorne AH, Meisen WH ... Toll-like Receptor 4 Ligands Down-regulate Fc? Receptor IIb (Fc?RIIb) via MARCH3 Protein-mediated Ubiquitination.. Fatehchand K ... Targeting myeloid-derived suppressor cells using a novel adenosine monophosphate-activated protein kinase (AMPK) activator.. ... Glycovariant anti-CD37 monospecific protein therapeutic exhibits enhanced effector cell-mediated cytotoxicity against chronic ...
more infohttps://cancer.osu.edu/research-and-education/find-a-researcher/search-researcher-directory/susheela-tridandapani

Dr. Jianhua  Yu - Cancer Researcher at OSUCCC - JamesDr. Jianhua Yu - Cancer Researcher at OSUCCC - James

Role of cysteine-rich 61 protein (CCN1) in macrophage-mediated oncolytic herpes simplex virus clearance.. Thorne AH, Meisen WH ... Bortezomib-induced unfolded protein response increases oncolytic HSV-1 replication resulting in synergistic antitumor effects. ... Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC - James) ... Russell L, Yoo JY, Bolyard CM, Lathia JD, Rich J, Puduvalli VK, Mao H, Yu J, Caligiuri MA, Tridandapani S, Kaur B ...
more infohttps://cancer.osu.edu/research-and-education/find-a-researcher/search-researcher-directory/jianhua-yu

In vivo - in vitro toxicogenomic comparison of TCDD-elicited gene expression in Hepa1c1c7 mouse hepatoma cells and C57BL/6...In vivo - in vitro toxicogenomic comparison of TCDD-elicited gene expression in Hepa1c1c7 mouse hepatoma cells and C57BL/6...

cysteine rich protein 61 Cyr61 16007 -1.6 2f 0.07 1.6 8, 48f ND ... small proline-rich protein 2D Sprr2d 20758 -1.6 18f, 72 1.97 ... secretory granule neuroendocrine protein 1, 7B2 protein Sgne1 20394 3.3 168f 0.74 -1.5 48f 175.00 ... mitochondrial ribosomal protein L37 Mrpl37 56280 8.3 2, 4, 8f, 12, 18, 24, 72, 168 8.77 2.7 2, 4f, 8, 12, 24, 48 49.59 ... whey acidic protein Wap 22373 5.9 2, 4, 8, 12, 18, 24, 72, 168f 0.12 4.2 2, 4, 8, 12, 24, 48f 17.44 ...
more infohttps://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-7-80/tables/1

Gene expression in murine mammary epithelial stem cell-like cells shows similarities to human breast cancer gene expression |...Gene expression in murine mammary epithelial stem cell-like cells shows similarities to human breast cancer gene expression |...

Cysteine-rich protein 61. -2.89. Regulation of cell growth/cell adhesion. Rrm1 ... proteolysis proteins (such as cathepsin D and cathepsin A) and transcription-related proteins (Ehf, confirmed by real-time PCR ... The protein concentration was determined with Bradford reagent (BioRad, Hercules, CA, USA). Whole-cell extracts (40 μg protein ... Confirmations at the protein level are depicted in Figures 2 to 5.. ...
more infohttps://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr2256

Che L[au] - PubMed - NCBIChe L[au] - PubMed - NCBI

Critical Role of Cysteine-Rich Protein 61 in Mediating the Activation of Renal Fibroblasts. ... PPR Protein BFA2 Is Essential for the Accumulation of the atpH/F Transcript in Chloroplasts. ... Axis inhibition protein 1 (Axin1) Deletion-Induced Hepatocarcinogenesis Requires Intact β-Catenin but Not Notch Cascade in Mice ... Mammary Protein Synthesis upon Long-Term Nutritional Restriction Was Attenuated by Oxidative-Stress-Induced Inhibition of ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Che+L%5Bau%5D&dispmax=50

Plus itPlus it

cysteine-rich angiogenic protein 61. FAAH. fatty acid amide hydrolase. FP. prostaglandin F2α. MAGL. monoacylglycerol lipase. PG ... a mitogen-activated protein kinase inhibitor can inhibit the antiadipogenic effect of both bimatoprost and PGF2α (Silvestri et ... γ and CCAAT enhancer-binding protein α in mouse as well as differentiating human adipocytes (Silvestri et al., 2013). In ... addition, late markers of adipocyte maturation such as fatty acid-binding protein 4 and adiponectin, as well as lipid ...
more infohttp://pharmrev.aspetjournals.org/content/65/4/1135

Iwasaki H[au] - PubMed - NCBIIwasaki H[au] - PubMed - NCBI

Urinary Cysteine-Rich Protein 61 and Trefoil Factor 3 as Diagnostic Biomarkers for Colorectal Cancer. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Iwasaki+H%5Bau%5D&dispmax=50

Association between genetic variants of reported candidate genes or regions and risk of cleft lip with or without cleft palate...Association between genetic variants of reported candidate genes or regions and risk of cleft lip with or without cleft palate...

Next article in issue: Embryonic expression of the cysteine rich protein 61 (CYR61) gene: A candidate for the development of ... Next article in issue: Embryonic expression of the cysteine rich protein 61 (CYR61) gene: A candidate for the development of ...
more infohttp://onlinelibrary.wiley.com/doi/10.1002/bdra.20687/abstract

The Molecular Basis for the Aging of Human Skin | Nova Science PublishersThe Molecular Basis for the Aging of Human Skin | Nova Science Publishers

Elevated cysteine-rich protein 61 (CCN1) promotes skin aging via upregulation of IL-1β in chronically sun-exposed human skin. ... Elevated cysteine-rich protein 61 (CCN1) promotes skin aging via upregulation of IL-1β in chronically sun-exposed human skin. ... Elevated cysteine-rich protein 61 (CCN1) promotes skin aging via upregulation of IL-1beta in chronically sun-exposed human skin ... Elevated cysteine-rich protein 61 (CCN1) promotes skin aging via upregulation of IL-1beta in chronically sun-exposed human skin ...
more infohttps://novapublishers.com/shop/the-molecular-basis-for-the-aging-of-human-skin/?add_to_wishlist=13678

Interleukin-1β, -6 and -8, EGR1, CYR61 and CTGF mRNA l | Open-iInterleukin-1β, -6 and -8, EGR1, CYR61 and CTGF mRNA l | Open-i

Cysteine-Rich Protein 61/genetics. *Disease Models, Animal. *Early Growth Response Protein 1/genetics ... We aimed to determine if the early response genes; connective tissue growth factor (CTGF), cysteine rich-61 (CYR61) and early ... We aimed to determine if the early response genes; connective tissue growth factor (CTGF), cysteine rich-61 (CYR61) and early ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2662809_1465-9921-10-19-7&req=4

Albert Hsiao - Google Scholar CitationsAlbert Hsiao - Google Scholar Citations

Thrombin receptor and RhoA mediate cell proliferation through integrins and cysteine-rich protein 61. CT Walsh, J Radeff-Huang ...
more infohttps://scholar.google.ca/citations?user=EJw4XO4AAAAJ&hl=en&oe=ASCII

Anti-CCN1/Cyr61 antibody (ab14237) | AbcamAnti-CCN1/Cyr61 antibody (ab14237) | Abcam

Cysteine rich heparin binding protein 61 antibody. *Cysteine-rich angiogenic inducer 61 antibody ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ... E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1/2000, Goat anti-IgY-HRP dilution: 1/1000. ... The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. 38:D142-D148 (2010) . ...
more infohttp://www.abcam.com/ccn1-antibody-ab14237.html

Anti-CCN1/Cyr61 antibody (ab10760) | AbcamAnti-CCN1/Cyr61 antibody (ab10760) | Abcam

Cysteine rich heparin binding protein 61 antibody. *Cysteine-rich angiogenic inducer 61 antibody ... Lysates/proteins at 10 µg per lane.. Secondary. All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 ... Lysates/proteins at 20 µg per lane.. Secondary. All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/5000 dilution. ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
more infohttp://www.abcam.com/ccn1-antibody-ab10760.html?productwalltab=abreviews

Håvard Attramadal
       - Institutt for klinisk medisinHåvard Attramadal - Institutt for klinisk medisin

... cysteine-rich protein 61 is released from activated platelets and increased in the circulation during experimentally induced ... The matri-cellular proteins cysteine-rich, angiogenic-inducer, 61 and connective tissue growth factor are regulated in ... The G-protein-coupled lactate receptor, GPR81 (HCA1), is known to promote lipid storage in adipocytes by downregulating cAMP ... G-protein-coupled receptor 81/hydroxycarboxylic acid receptor 1: Expression and action in brain. Journal of Neuroscience ...
more infohttps://www.med.uio.no/klinmed/personer/vit/havarda/index.html

Mutagenetix > Incidental...Mutagenetix > Incidental...

cysteine rich protein 61. Synonyms. CCN1. MMRRC Submission. Accession Numbers. Is this an essential gene?. Possibly non ... The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in ... This summary is for the human ortholog.] The secreted protein encoded by this gene is growth factor-inducible and promotes the ...
more infohttp://mutagenetix.utsouthwestern.edu/incidental/incidental_rec.cfm?mid=552631
  • These proteins, together with WISP1 (CCN4), WISP2 (CCN5), and WISP3 (CCN6) comprise the six members of the family in vertebrates and have been renamed CCN1-6 in order of their discovery by international consensus. (wikipedia.org)
  • The 5th exon also contains the 3'-UTR sequences, which has 5 copies of AU-rich elements that confers a short mRNA half life, and a mir-155 target site. (wikipedia.org)
  • Chin, LH , Hsu, SP , Zhong, W-B & Liang, YC 2016, ' Involvement of cysteine-rich protein 61 in the epidermal growth factor-induced migration of human anaplastic thyroid cancer cells ', Molecular Carcinogenesis . (elsevier.com)
  • Activation of cell survival pathways such as phosphoinositide-3-kinase/AKT/IKK or RAS/mitogen-activated protein kinase phosphorylates FOXOs at different sites which regulate FOXOs nuclear localization or degradation. (hindawi.com)
  • siRNA-mediated Ezrin knockdown decreased fibroblast size and mechanical properties, and thus impaired the nuclear translocation of YAP, a protein commonly response to cell size and mechanical force. (springer.com)
  • The concentration gradients of the kit standards or positive controls render a theoretical kit detection range of 3.12ng/ml-100ng/ml in biological research samples containing Cyr 61, with an estimated sensitivity of 1.0ng/ml. (mybiosource.com)
  • The transport of FOXO proteins through the nuclear pore is dependent on active-transport mechanisms. (hindawi.com)
  • Any process that stops, prevents, or reduces the frequency, rate or extent of cysteine-type endopeptidase activity. (princeton.edu)
  • CCN proteins likely constitute a hub for the coordination of cell signaling and communication. (wikipedia.org)
  • "Comparative genomic analysis of equilibrative nucleoside transporters suggests conserved protein structure despite limited sequence identity. (tcdb.org)