A family of peptidyl-prolyl cis-trans isomerases that bind to CYCLOSPORINS and regulate the IMMUNE SYSTEM. EC 5.2.1.-
An enzyme that catalyzes the isomerization of proline residues within proteins. EC 5.2.1.8.
A 17-KDa cytoplasmic PEPTIDYLPROLYL ISOMERASE involved in immunoregulation. It is a member of the cyclophilin family of proteins that binds to CYCLOSPORINE.
Enzymes that catalyze either the racemization or epimerization of chiral centers within amino acids or derivatives. EC 5.1.1.
A cyclic undecapeptide from an extract of soil fungi. It is a powerful immunosupressant with a specific action on T-lymphocytes. It is used for the prophylaxis of graft rejection in organ and tissue transplantation. (From Martindale, The Extra Pharmacopoeia, 30th ed).
Members of a family of highly conserved proteins which are all cis-trans peptidyl-prolyl isomerases (PEPTIDYLPROLYL ISOMERASE). They bind the immunosuppressant drugs CYCLOSPORINE; TACROLIMUS and SIROLIMUS. They possess rotamase activity, which is inhibited by the immunosuppressant drugs that bind to them.
A widely distributed cell surface transmembrane glycoprotein that stimulates the synthesis of MATRIX METALLOPROTEINASES. It is found at high levels on the surface of malignant NEOPLASMS and may play a role as a mediator of malignant cell behavior.
A family of immunophilin proteins that bind to the immunosuppressive drugs TACROLIMUS (also known as FK506) and SIROLIMUS. EC 5.2.1.-
A group of closely related cyclic undecapeptides from the fungi Trichoderma polysporum and Cylindocarpon lucidum. They have some antineoplastic and antifungal action and significant immunosuppressive effects. Cyclosporins have been proposed as adjuvants in tissue and organ transplantation to suppress graft rejection.
An actinomycete from which the antibiotic OLEANDOMYCIN is obtained.
Biological properties, processes, and activities of VIRUSES.
Transport proteins that carry specific substances in the blood or across cell membranes.
A CALCIUM and CALMODULIN-dependent serine/threonine protein phosphatase that is composed of the calcineurin A catalytic subunit and the calcineurin B regulatory subunit. Calcineurin has been shown to dephosphorylate a number of phosphoproteins including HISTONES; MYOSIN LIGHT CHAIN; and the regulatory subunits of CAMP-DEPENDENT PROTEIN KINASES. It is involved in the regulation of signal transduction and is the target of an important class of immunophilin-immunosuppressive drug complexes.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A macrolide isolated from the culture broth of a strain of Streptomyces tsukubaensis that has strong immunosuppressive activity in vivo and prevents the activation of T-lymphocytes in response to antigenic or mitogenic stimulation in vitro.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Proteins prepared by recombinant DNA technology.
The interactions between a host and a pathogen, usually resulting in disease.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
A constellation of responses that occur when an organism is exposed to excessive heat. Responses include synthesis of new proteins and regulation of others.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Agents that suppress immune function by one of several mechanisms of action. Classical cytotoxic immunosuppressants act by inhibiting DNA synthesis. Others may act through activation of T-CELLS or by inhibiting the activation of HELPER CELLS. While immunosuppression has been brought about in the past primarily to prevent rejection of transplanted organs, new applications involving mediation of the effects of INTERLEUKINS and other CYTOKINES are emerging.
A genus of FLAVIVIRIDAE causing parenterally-transmitted HEPATITIS C which is associated with transfusions and drug abuse. Hepatitis C virus is the type species.
That part of the genome that corresponds to the complete complement of EXONS of an organism or cell.
A technique in which electric pulses of intensity in kilovolts per centimeter and of microsecond-to-millisecond duration cause a temporary loss of the semipermeability of CELL MEMBRANES, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Proteins that form the structure of the NUCLEAR PORE. They are involved in active, facilitated and passive transport of molecules in and out of the CELL NUCLEUS.
An opening through the NUCLEAR ENVELOPE formed by the nuclear pore complex which transports nuclear proteins or RNA into or out of the CELL NUCLEUS and which, under some conditions, acts as an ion channel.
Nucleocytoplasmic transport molecules that bind to ALPHA KARYOPHERINS in the CYTOSOL and are involved in transport of molecules through the NUCLEAR PORE COMPLEX. Once inside the CELL NUCLEUS beta karyopherins interact with RAN GTP-BINDING PROTEIN and dissociate from alpha karyopherins. Beta karyopherins bound to RAN GTP-BINDING PROTEIN are then re-transported to the cytoplasm where hydrolysis of the GTP of RAN GTP-BINDING PROTEIN causes release of karyopherin beta.
Nucleocytoplasmic transport molecules that bind to the NUCLEAR LOCALIZATION SIGNALS of cytoplasmic molecules destined to be imported into the CELL NUCLEUS. Once attached to their cargo they bind to BETA KARYOPHERINS and are transported through the NUCLEAR PORE COMPLEX. Inside the CELL NUCLEUS alpha karyopherins dissociate from beta karypherins and their cargo. They then form a complex with CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN and RAN GTP-BINDING PROTEIN which is exported to the CYTOPLASM.
Gated transport mechanisms by which proteins or RNA are moved across the NUCLEAR MEMBRANE.
A family of proteins involved in NUCLEOCYTOPLASMIC TRANSPORT. Karyopherins are heteromeric molecules composed two major types of components, ALPHA KARYOPHERINS and BETA KARYOPHERINS, that function together to transport molecules through the NUCLEAR PORE COMPLEX. Several other proteins such as RAN GTP BINDING PROTEIN and CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN bind to karyopherins and participate in the transport process.
A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)
Semiautonomous, self-reproducing organelles that occur in the cytoplasm of all cells of most, but not all, eukaryotes. Each mitochondrion is surrounded by a double limiting membrane. The inner membrane is highly invaginated, and its projections are called cristae. Mitochondria are the sites of the reactions of oxidative phosphorylation, which result in the formation of ATP. They contain distinctive RIBOSOMES, transfer RNAs (RNA, TRANSFER); AMINO ACYL T RNA SYNTHETASES; and elongation and termination factors. Mitochondria depend upon genes within the nucleus of the cells in which they reside for many essential messenger RNAs (RNA, MESSENGER). Mitochondria are believed to have arisen from aerobic bacteria that established a symbiotic relationship with primitive protoeukaryotes. (King & Stansfield, A Dictionary of Genetics, 4th ed)
The process whereby calcium salts are deposited in the dental enamel. The process is normal in the development of bones and teeth. (Boucher's Clinical Dental Terminology, 4th ed, p43)
Dentin formed by normal pulp after completion of root end formation.
The type species of the genus INFLUENZAVIRUS A that causes influenza and other diseases in humans and animals. Antigenic variation occurs frequently between strains, allowing classification into subtypes and variants. Transmission is usually by aerosol (human and most non-aquatic hosts) or waterborne (ducks). Infected birds shed the virus in their saliva, nasal secretions, and feces.
An acute viral infection in humans involving the respiratory tract. It is marked by inflammation of the NASAL MUCOSA; the PHARYNX; and conjunctiva, and by headache and severe, often generalized, myalgia.
A subtype of INFLUENZA A VIRUS with the surface proteins hemagglutinin 1 and neuraminidase 1. The H1N1 subtype was responsible for the Spanish flu pandemic of 1918.
Proteins involved in the transport of specific substances across the membranes of the MITOCHONDRIA.
Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.
The mitochondria of the myocardium.
An increase in MITOCHONDRIAL VOLUME due to an influx of fluid; it occurs in hypotonic solutions due to osmotic pressure and in isotonic solutions as a result of altered permeability of the membranes of respiring mitochondria.
Damage to the MYOCARDIUM resulting from MYOCARDIAL REPERFUSION (restoration of blood flow to ischemic areas of the HEART.) Reperfusion takes place when there is spontaneous thrombolysis, THROMBOLYTIC THERAPY, collateral flow from other coronary vascular beds, or reversal of vasospasm.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
A disorder of cardiac function caused by insufficient blood flow to the muscle tissue of the heart. The decreased blood flow may be due to narrowing of the coronary arteries (CORONARY ARTERY DISEASE), to obstruction by a thrombus (CORONARY THROMBOSIS), or less commonly, to diffuse narrowing of arterioles and other small vessels within the heart. Severe interruption of the blood supply to the myocardial tissue may result in necrosis of cardiac muscle (MYOCARDIAL INFARCTION).
Striated muscle cells found in the heart. They are derived from cardiac myoblasts (MYOBLASTS, CARDIAC).
The units based on political theory and chosen by countries under which their governmental power is organized and administered to their citizens.
A class of MOLECULAR CHAPERONES found in both prokaryotes and in several compartments of eukaryotic cells. These proteins can interact with polypeptides during a variety of assembly processes in such a way as to prevent the formation of nonfunctional structures.

Two distinct regions of cyclophilin B are involved in the recognition of a functional receptor and of glycosaminoglycans on T lymphocytes. (1/501)

Cyclophilin B is a cyclosporin A-binding protein exhibiting peptidyl-prolyl cis/trans isomerase activity. We have previously shown that it interacts with two types of binding sites on T lymphocytes. The type I sites correspond to specific functional receptors and the type II sites to sulfated glycosaminoglycans. The interactions of cyclophilin B with type I and type II sites are reduced in the presence of cyclosporin A and of a synthetic peptide mimicking the N-terminal part of cyclophilin B, respectively, suggesting that the protein possesses two distinct binding regions. In this study, we intended to characterize the areas of cyclophilin B involved in the interactions with binding sites present on Jurkat cells. The use of cyclophilin B mutants modified in the N-terminal region demonstrated that the 3Lys-Lys-Lys5 and 14Tyr-Phe-Asp16 clusters are probably solely required for the interactions with the type II sites. We further engineered mutants of the conserved central core of cyclophilin B, which bears the catalytic and the cyclosporin A binding sites as an approach to localize the binding regions for the type I sites. The enzymatic activity of cyclophilin B was dramatically reduced after substitution of the Arg62 and Phe67 residues, whereas the cyclosporin A binding activity was destroyed by mutation of the Trp128 residue and strongly decreased after modification of the Phe67 residue. Only the substitution of the Trp128 residue reduced the binding of the resulting cyclophilin B mutant to type I binding sites. The catalytic site of cyclophilin B therefore did not seem to be essential for cellular binding and the cyclosporin A binding site appeared to be partially involved in the binding to type I sites.  (+info)

Evidence that cyclophilin-A protects cells against oxidative stress. (2/501)

Cyclophilin-A is the cytosolic isoform of a family of peptidylproline cis-trans-isomerases that bind cyclosporin A. This study investigates the role of cyclophilin-A in necrotic cell death, induced by 'chemical ischaemia' and by t-butylhydroperoxide. An 18-mer antisense phosphorothioate oligodeoxynucleotide was used to target a translated region of cyclophilin-A mRNA in rat neonatal cardiomyocytes. After a 24 h exposure to the oligonucleotide, the amount of cyclophilin-A in the cells was decreased by at least 93% as judged by immunological and enzymic criteria. For the enzyme assays, peptidyl proline cis-trans-isomerase activity was measured fluorimetrically in small (10 microl) volumes of cell extract. Immunoblots were developed with a polyclonal anti-cyclophilin-A antibody after sample isoelectric focusing and SDS/PAGE. Cyclophilin-A suppression had no effect on cyanide-plus-2-deoxyglucose-induced cell death. However, cyclophilin-A-suppressed cells were markedly more sensitive to t-butylhydroperoxide. Cyclosporin A conferred some resistance to the peroxide in both types of cell, but protection was greater in cyclophilin-A-suppressed cells, where cyclosporin A increased the survival time 2-fold. It is concluded that two cyclophilin isoforms are involved, in quite different ways, in peroxide-induced cell death. Cyclophilin-A has a protective role. Another isoform, possibly mitochondrial cyclophilin-D, has a deleterious role, such that blockade by cyclosporin A leads to protection.  (+info)

Polypeptide flux through bacterial Hsp70: DnaK cooperates with trigger factor in chaperoning nascent chains. (3/501)

A role for DnaK, the major E. coli Hsp70, in chaperoning de novo protein folding has remained elusive. Here we show that under nonstress conditions DnaK transiently associates with a wide variety of nascent and newly synthesized polypeptides, with a preference for chains larger than 30 kDa. Deletion of the nonessential gene encoding trigger factor, a ribosome-associated chaperone, results in a doubling of the fraction of nascent polypeptides interacting with DnaK. Combined deletion of the trigger factor and DnaK genes is lethal under normal growth conditions. These findings indicate important, partially overlapping functions of DnaK and trigger factor in de novo protein folding and explain why the loss of either chaperone can be tolerated by E. coli.  (+info)

The mitochondrial permeability transition pore and its role in cell death. (4/501)

This article reviews the involvement of the mitochondrial permeability transition pore in necrotic and apoptotic cell death. The pore is formed from a complex of the voltage-dependent anion channel (VDAC), the adenine nucleotide translocase and cyclophilin-D (CyP-D) at contact sites between the mitochondrial outer and inner membranes. In vitro, under pseudopathological conditions of oxidative stress, relatively high Ca2+ and low ATP, the complex flickers into an open-pore state allowing free diffusion of low-Mr solutes across the inner membrane. These conditions correspond to those that unfold during tissue ischaemia and reperfusion, suggesting that pore opening may be an important factor in the pathogenesis of necrotic cell death following ischaemia/reperfusion. Evidence that the pore does open during ischaemia/reperfusion is discussed. There are also strong indications that the VDAC-adenine nucleotide translocase-CyP-D complex can recruit a number of other proteins, including Bax, and that the complex is utilized in some capacity during apoptosis. The apoptotic pathway is amplified by the release of apoptogenic proteins from the mitochondrial intermembrane space, including cytochrome c, apoptosis-inducing factor and some procaspases. Current evidence that the pore complex is involved in outer-membrane rupture and release of these proteins during programmed cell death is reviewed, along with indications that transient pore opening may provoke 'accidental' apoptosis.  (+info)

Import and processing of heart mitochondrial cyclophilin D. (5/501)

Cyclophilins are a family of cyclosporin-A-binding proteins which catalyse rotation about prolyl peptide bonds. A mitochondrial isoform in mammalian cells, cyclophilin D, is a component of the permeability transition pore that is formed by the adenine nucleotide translocase and the voltage-dependent anion channel at contact sites between the inner and outer membrane. This study investigated the submitochondrial location of cyclophilin D by following the fate of radiolabelled protein following import. Precursor [(35)S]cyclophilin D was expressed in vitro from a PCR-generated cDNA. The precursor was imported by rat heart mitochondria and processed in a single step to a 21-kDa protein that was identical (SDS/PAGE) to an in vitro expressed mature protein and a cyclophilin D purified from rat heart mitochondria. No further modification of the mature protein could be demonstrated. Fractionation of mitochondria following import established that cyclophilin D locates only to the matrix. It is concluded that cyclophilin D binding to the permeability transition pore must occur at the inner face of the mitochondrial inner membrane.  (+info)

Cyclophilin B binding to platelets supports calcium-dependent adhesion to collagen. (6/501)

We have recently reported that cyclophilin B (CyPB), a secreted cyclosporine-binding protein, could bind to T lymphocytes through interactions with two types of binding sites. The first ones, referred to as type I, involve interactions with the conserved domain of CyPB and promote the endocytosis of surface-bound ligand, while the second type of binding sites, termed type II, are represented by glycosaminoglycans (GAG). Here, we further investigated the interactions of CyPB with blood cell populations. In addition to lymphocytes, CyPB was found to interact mainly with platelets. The binding is specific, with a dissociation constant (kd) of 9 +/- 3 nmol/L and the number of sites estimated at 960 +/- 60 per cell. Platelet glycosaminoglycans are not required for the interactions, but the binding is dramatically reduced by active cyclosporine derivatives. We then analyzed the biologic effects of CyPB and found a significant increase in platelet adhesion to collagen. Concurrently, CyPB initiates a transmembranous influx of Ca(2+) and induces the phosphorylation of the P-20 light chains of myosin. Taken together, the present results demonstrate for the first time that extracellular CyPB specifically interacts with platelets through a functional receptor related to the lymphocyte type I binding sites and might act by regulating the activity of a receptor-operated membrane Ca(2+) channel.  (+info)

A cyclophilin B gene encodes antigenic epitopes recognized by HLA-A24-restricted and tumor-specific CTLs. (7/501)

We have studied Ags recognized by HLA class I-restricted CTLs established from tumor site to better understand the molecular basis of tumor immunology. HLA-A24-restricted and tumor-specific CTLs established from T cells infiltrating into lung adenocarcinoma recognized the two antigenic peptides encoded by a cyclophilin B gene, a family of genes for cyclophilins involved in T cell activation. These two cyclophilin B peptides at positions 84-92 and 91-99 induced HLA-A24-restricted CTL activity against tumor cells in PBMCs of leukemia patients, but not in epithelial cancer patients or in healthy donors. In contrast, the modified peptides at position 2 from phenylalanine to tyrosine, which had more than 10 times higher binding affinities to HLA-A24 molecules, could induce HLA-A24-restricted CTL activity against tumor cells in PBMCs from leukemia patients, epithelial cancer patients, or healthy donors. PHA-activated normal T cells were resistant to lysis by the CTL line or by these peptide-induced CTLs. These results indicate that a cyclophilin B gene encodes antigenic epitopes recognized by CTLs at the tumor site, although T cells in peripheral blood (except for those from leukemia patients) are immunologically tolerant to the cyclophilin B. These peptides might be applicable for use in specific immunotherapy of leukemia patients or that of epithelial cancer patients.  (+info)

Adenine nucleotide translocase-1, a component of the permeability transition pore, can dominantly induce apoptosis. (8/501)

Here, we describe the isolation of adenine nucleotide translocase-1 (ANT-1) in a screen for dominant, apoptosis-inducing genes. ANT-1 is a component of the mitochondrial permeability transition complex, a protein aggregate connecting the inner with the outer mitochondrial membrane that has recently been implicated in apoptosis. ANT-1 expression led to all features of apoptosis, such as phenotypic alterations, collapse of the mitochondrial membrane potential, cytochrome c release, caspase activation, and DNA degradation. Both point mutations that impair ANT-1 in its known activity to transport ADP and ATP as well as the NH(2)-terminal half of the protein could still induce apoptosis. Interestingly, ANT-2, a highly homologous protein could not lead to cell death, demonstrating the specificity of the signal for apoptosis induction. In contrast to Bax, a proapoptotic Bcl-2 gene, ANT-1 was unable to elicit a form of cell death in yeast. This and the observed repression of apoptosis by the ANT-1-interacting protein cyclophilin D suggest that the suicidal effect of ANT-1 is mediated by specific protein-protein interactions within the permeability transition pore.  (+info)

SR GROUP - Exporter, Importer, Manufacturer, Distributor, Supplier, Trading Company of Rat CYPB(Cyclophilin B) ELISA Kit based in Delhi, India
TY - JOUR. T1 - Cyclophilin B expression is associated with in vitro radioresistance and clinical outcome after radiotherapy. AU - Williams, Paul D.. AU - Owens, Charles R.. AU - Dziegielewski, Jaroslaw. AU - Moskaluk, Christopher A.. AU - Read, Paul W.. AU - Larner, James M.. AU - Story, Michael D.. AU - Brock, William A.. AU - Amundson, Sally A.. AU - Lee, Jae K.. AU - Theodorescu, Dan. N1 - Funding Information: Abbreviations: PPIB, cyclophilin B; COXEN, Coexpression Extrapolation; GEM, gene expression model Address all correspondence to: Dan Theodorescu, MD, PhD, Departments of Surgery and Pharmacology and Comprehensive Cancer Center, University of Colorado, 13001 E 17th Pl MS #F-434, Aurora, CO 80045. E-mail: [email protected] 1This work was supported in part by National Institutes of Health grants DK069264 to P.D.W., CA06294 to M.D.S. and W.A.B., HL081690 to J.K.L., CA075115 to D.T., and U19AI067773 and US Department of Energy DE-FG02-07ER46336 to S.A.A. 2This article refers to ...
Hepatic fibrosis can result as a pathological response to nonalcoholic steatohepatitis (NASH). Cirrhosis, the late stage of fibrosis, has been linked to poor survival and an increased risk of developing hepatocellular carcinoma, with limited treatment options available. Therefore, there is an unmet …
A critical role of Cyclophilins, mostly Cyclophilin A (CyPA), in the replication of HCV is supported by a growing body of in vitro and in vivo evidence. CyPA probably interacts directly with nonstructural protein 5A to exert its effect, through its peptidyl-prolyl isomerase activity, on maintaining the proper structure and function of the HCV replicase. The major proline substrates are located in domain II of NS5A, centered around a
The aim of the research was to determine the influence of the substrate and different drainage materials on retention capacity and runoff water quality from three green roof containers. Phosphates were chosen as the water quality indicator based on their potential adverse impact on water quality in …
Restoration of blood flow after myocardial infarction (MI), surgery or fibrinolytic therapy is necessary, but can lead to cardiomyocyte dysfunction within a generalised condition commonly known as reperfusion injury. The role of cyclophilins, heat shock proteins (HSP), and the mitochondrial chaperone complex (MCC), was studied in this pathological condition. In vitro and in vivo models were used to replicate conditions of ischaemia/reperfusion (IR) injury. H9c2 and COS-7 cell lines were employed in nitric oxide (NO) donor and transfection applications. Experimental protocols were used to determine mitochondrial membrane potential (MMP), mitochondrial morphology, protein expression, enzyme activity and cell damage in these models. No difference was observed in activity or expression in cyclophilin or expression of the MCC in any of the models. It was noted that in the in vitro model, cell death was predominantly necrotic with only a minority of cells undergoing apoptosis, and as the degree of ...
TY - JOUR. T1 - Novel approach to inhibit asthma-mediated lung inflammation using Anti-CD147 intervention. AU - Gwinn, William M.. AU - Damsker, Jesse M.. AU - Falahati, Rustom. AU - Okwumabua, Ifeanyi. AU - Kelly-Welch, Ann. AU - Keegan, Achsah D.. AU - Vanpouille, Christophe. AU - Lee, James J.. AU - Dent, Lindsay A.. AU - Leitenberg, David. AU - Bukrinsky, Michael I.. AU - Constant, Stephanie L.. PY - 2006/10/1. Y1 - 2006/10/1. N2 - Extracellular cyclophilins have been well described as chemotactic factors for various leukocyte subsets. This chemotactic capacity is dependent upon interaction of cyclophilins with the cell surface signaling receptor CD147. Elevated levels of extracellular cyclophilins have been documented in several inflammatory diseases. We propose that extracellular cyclophilins, via interaction with CD147, may contribute to the recruitment of leukocytes from the periphery into tissues during inflammatory responses. In this study, we examined whether extracellular ...
A validated positive silencing control targeting the Cyclophilin B (PPIB) gene in human, mouse, or rat cell lines. Useful for determination of optimal RNAi experimental conditions
The mitochondrial permeability transition pore (PTP) has been established as an important mediator of ischemia-reperfusion-induced cell death. The matrix protein cyclophilin D (CypD) is the best known regulator of PTP opening. Therefore, the authors hypothesized that isoflurane, by inhibiting the re...
Cyclophilin B, 0.1 ml. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Research and training opportunities are available in a laboratory of the National Research Council of Italy for a postgraduate or postdoctoral student in the field of molecular parasitology. The laboratory is about to be relocated near Rome, in a multidisciplinary research complex comprising a European facility for the study of mouse mutants and four research groups of the EMBL. The proposed project is based on the study of cyclophilins (cyclosporin binding proteins) of the human parasites belonging to the genus Schistosoma. Molecular cloning and biochemical studies of schistosome cyclophilins are already under way in the host laboratory. An interest in cyclophilins is due to their likely role in mediating the antiparasitic effects of cyclosporin A and its derivatives. Supervision and research facilities will be provided to students who have been successful in applying to the Training and Mobility of Researchers Programme of the European Community (see the call for proposals under ...
Retraction of: J. Cell Sci. 123, 4117-4127. This article has been retracted at the request of the corresponding author, John G. Pastorino.. This notice updates and replaces a recent Expression of Concern, published on 15 February 2016.. Journal of Cell Science was alerted to potential blot duplication and reuse in the following five papers published in Journal of Cell Science by John G. Pastorino:. Sirtuin-3 deacetylation of cyclophilin D induces dissociation of hexokinase II from the mitochondria Nataly Shulga, Robin Wilson-Smith and John G. Pastorino J. Cell. Sci. (2010) 123, 894-902. Ethanol sensitizes mitochondria to the permeability transition by inhibiting deacetylation of cyclophilin-D mediated by sirtuin-3 Nataly Shulga and John G. Pastorino J. Cell. Sci. (2010) 123, 4117-4127. GRIM-19-mediated translocation of STAT3 to mitochondria is necessary for TNF-induced necroptosis Nataly Shulga and John G. Pastorino J. Cell. Sci. (2012) 125, 2995-3003. Sirtuin-3 modulates Bak- and Bax-dependent ...
Polyclonal antibody for Cyclophilin B/PPIB detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Cyclophilin B/PPIB information: Molecular Weight: 23743 MW; Subcellular Localization: Endoplasmic reticulum lumen. Me
Although members of the Flaviviridae display high incidence, morbidity, and mortality rates, the development of specific antiviral drugs for each virus is
Cyclophilin-D Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 178 aa having a molecular mass of 18.9kDa.
3RCL: Fragment-based discovery of a new family of non-peptidic small-molecule cyclophilin inhibitors with potent antiviral activities.
2004). Patz EF Jr: Translating biomarkers into clinical practice: prognostic implications of cyclophilin A and macrophage migratory inhibitory factor identified from protein expression profiles in non-small cell lung cancer. Lung Cancer ...
ProSpecs Cyclophilins include: Cyclophilin-A Human Recombinant, Cyclophilin-B Human Recombinant, Cyclophilin-D Human Recombinant
The spliceosome is a complex and dynamic collection of RNA and proteins that removes introns from precursor mRNA transcripts. Alterations in the splicing machin...
SWISS-MODEL Template Library (SMTL) entry for 5ta4.1. Discovery of a Potent Cyclophilin Inhibitor (Compound 8) based on Structural Simplification of Sanglifehrin A
Cyclophilin C-associated protein (CyCAP) has been proposed as the endogenous equivalent of the immunosuppressant drug, cyclosporin A. It competes with cyclosporin A for binding to cyclophilin C. It is also known as lectin, galactoside-binding, soluble, 3 binding protein (Lgals3bp); Cyp-C-associated protein, 90K, Ppicap, and MAC-2BP. CyCAP expression has been reported in brain, kidney, macrophage cells, dermal fibroblasts, and keratinocytes. Roles for CyCAP have been reported in wound healing and macrophage activation via association with nuclear factor of activated T-cells (NFAT).. ...
Cyclophilin C-associated protein (CyCAP) has been proposed as the endogenous equivalent of the immunosuppressant drug, cyclosporin A. It competes with cyclosporin A for binding to cyclophilin C. It is also known as lectin, galactoside-binding, soluble, 3 binding protein (Lgals3bp); Cyp-C-associated protein, 90K, Ppicap, and MAC-2BP. CyCAP expression has been reported in brain, kidney, macrophage cells, dermal fibroblasts, and keratinocytes. Roles for CyCAP have been reported in wound healing and macrophage activation via association with nuclear factor of activated T-cells (NFAT).. ...
Debio: 1347-201: A phase 2 basket study of the oral selective pan-FGFR inhibitor Debio 1347 in subjects with solid tumors harboring a fusion of FGFR1, FGFR2 or FGFR3 The FUZE Clinical Trial
Mölnlycke Exufiber gelling fibre dressing with Hydrolock technology and advanced fluid retention capacity for treating highly exuding and cavity wounds 
SEBake PF can be used to strengthen the dough, improve dough mixing tolerance & machinability, as well as to enhance the gas retention capacity of the dough.. ...
A stable, fluorescent positive control suitable for RNAi experiments in human, mouse, or rat cells Silences the Cyclophilin B gene and is labeled with DY-547
OriGene Anti-PPIF Monoclonal (OTI1G8), TrueMAB™, Catalog # CF809070. Tested in Western Blot (WB) and Immunohistochemistry (Paraffin) (IHC (P)) applications. This antibody reacts with Human samples. Supplied as 100 µg purified antibody.
Author Summary Cyclophilins are proteins that catalyze the isomerization of prolines, interconverting this structurally important amino acid between cis and trans isomers. Although there are 17 cyclophilins in the human genome, the function of most cyclophilin isoforms is unknown. At least some members of this protein family are of interest for clinically relevant drug design, as they are targets of the drug cyclosporin, which is used as an immunosuppressant to treat patients following organ transplantation. The absence of a comprehensive picture of the similarities and differences between the different members of this protein family precludes effective and specific drug design, however. In the current study we undertake such a global structure∶function analysis. Using biochemical, structural, and computational methods we characterize the human cyclophilin family in detail and suggest that there is a previously overlooked region of these enzymes that contributes significantly to isoform diversity. We
Author Summary Cyclophilins are proteins that catalyze the isomerization of prolines, interconverting this structurally important amino acid between cis and trans isomers. Although there are 17 cyclophilins in the human genome, the function of most cyclophilin isoforms is unknown. At least some members of this protein family are of interest for clinically relevant drug design, as they are targets of the drug cyclosporin, which is used as an immunosuppressant to treat patients following organ transplantation. The absence of a comprehensive picture of the similarities and differences between the different members of this protein family precludes effective and specific drug design, however. In the current study we undertake such a global structure∶function analysis. Using biochemical, structural, and computational methods we characterize the human cyclophilin family in detail and suggest that there is a previously overlooked region of these enzymes that contributes significantly to isoform diversity. We
The mitochondrial permeability transition pore (mPTP) plays a critical role in the pathogenesis of cardiovascular diseases, including ischemia/reperfusion injury. Although the pore structure is still unresolved, the mechanism through which cyclophilin D (CypD) regulates mPTP opening is the subject of intensive studies. While post-translational modifications of CypD have been shown to modulate pore opening, specific phosphorylation sites of CypD have not yet been identified. We hypothesized here that phosphorylation of CypD on a serine residue controls mPTP opening and subsequent cell death at reperfusion. We combined in silico analysis with in vitro and genetic manipulations to determine potential CypD phosphorylation sites and their effect on mitochondrial function and cell death. Importantly, we developed an in vivo intramyocardial adenoviral strategy to assess the effect of the CypD phosphorylation event on infarct size. Our results show that although CypD can potentially be phosphorylated at
eng] Cyclophilin-D (CyP-D) is a peptidyl prolyl cis/trans isomerase located in the mitochondrial matrix of mammalian cells. The subcellular localization of the protein is determined by the presence of a mitochondrial targeting presequence. In the first part of this work, we characterized human CyP-D presequence allowing the protein translocation into mitochondria. We showed that the 16 first amino acid of the presequence are necessary and sufficient to form a functional presequence and to address hCyP-D into mitochondria. One of the main physiological roles of CyP-D is to activate the mitochondrial permeability transition pore (mPTP) opening. The mPTP is a protein complex formed during oxidative stress and leading to cell necrosis. Thus, CyP-D may be considered as a necrosis inductor. Nevertheless, several studies have also shown that CyP-D exhibits a protective role toward apoptosis induced by oxidative stress. However, the mechanism implicated in the cellular protection conferred by CyP-D is ...
Numerous mechanisms have been suggested for how bacterial toxins kill susceptible mammalian cells. Several recent studies demonstrated the importance of mitochondrial targeting of toxins produced by H. pylori, C. difficile, and S. aureus to mitochondria (56). In these cases toxin-mediated cell death was caspase independent and did not result in typical PTPs in the MOM (14, 18).. Previously, we reported that M. haemolytica LKT induces apoptosis of BL-3 cells in a caspase-9-dependent manner and that in mitochondria isolated from LKT-intoxicated BL-3 cells there was gross damage to the MOM (4). Based on these observations, we hypothesized that LKT is transported into the cell and binds directly to mitochondria. In the present study, we first demonstrated that full-length LKT protein could be identified in purified mitochondrial lysates from LKT-treated BL-3 cells (Fig. 1A). Transfection of anti-LKT antibodies into BL-3 cells prevented binding of LKT to mitochondria. Confocal microscopy and flow ...
Mitochondrial permeability transition pore component cyclophilin D distinguishes nigrostriatal dopaminergic death paradigms in the MPTP mouse model of Parkinsons disease Academic Article ...
PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.
Recombinant Peptidylprolyl Isomerase B (Cyclophilin B) (PPIB) Peptide. Species: Human. Source: Escherichia coli (E. coli). Order product ABIN934947.
An absorbent structure made at least in part from a superabsorbent material having a retention capacity (CRC) as determined by a Centrifuge Retention Capacity Test of at least about 25 g/g and a free swell gel bed permeability (GBP) as determined by a Free Swell Gel Bed Permeability Test of at least 575 10−9 cm2. In another embodiment, the absorbent structure is made at least in part from a superabsorbent material having a retention capacity (CRC) as determined by a Centrifuge Retention Capacity Test of at least about 25 g/g, an absorbency under load (AUL) at 0.9 psi as determined by an Absorbency Under Load Test of at least 18 and a free swell gel bed permeability (GBP) as determined by a Free Swell Gel Bed Permeability Test of at least about 350 10−9 cm2.
article{203b97eb-96fd-4fd8-a31f-dd57ba747ae5, abstract = {,p,Mitochondria control bioenergetics and cell fate decisions, but how they influence nuclear gene expression is understood poorly. Here, we show that deletion or reduction in the levels of cyclophilin D (CypD, also called Ppif), a mitochondrial matrix peptidyl prolyl isomerase and apoptosis regulator, results in increased cell proliferation and enhanced cell migration and invasion. These responses are associated with extensive transcriptional changes, modulation of a chemokine/chemokine receptor gene signature, and activation of the pleiotropic inflammatory mediator, STAT3. In the absence of CypD, active STAT3 enhances cell proliferation via accelerated entry into S-phase and stimulates autocrine/paracrine cell motility through Cxcl12-Cxcr4-directed chemotaxis. Therefore, CypD directs mitochondria-to-nuclei inflammatory gene expression in normal and tumor cells. This pathway may contribute to malignant traits under conditions of CypD ...
The mechanism by which cyclosporin A (CsA) inhibits vaccinia virus (VV) replication is still unclear. The present study addresses the question of whether CsA-binding proteins named cyclophilins (Cyps) are involved in the anti-VV activity of CsA. Six CsA analogues were analysed, and their affinity for Cyps in VV-infected BSC-40 cells and their potency as inhibitors of VV replication were evaluated. It was demonstrated that analogues with strong Cyp-binding activity, such as CsC, CsG and [MeAla6]CsA, also exhibit a strong antiviral effect. In contrast, drugs with low ([MeBm2t1]CsA and CsH) or no ([MeLeu11]CsA) affinity for Cyps show poor or no antiviral activity. The data obtained suggest a correlation between the ability of CsA to block VV replication and Cyp binding activity, and indicate the involvement of Cyps in the VV replicative cycle. They also suggest that the anti-VV action of CsA may occur by a pathway distinct from that involved in the immunosuppressive effect of the drug.
Drug-induced mitochondrial dysfunction has been implicated in many types of organ toxicity, including liver and intestine. The induction of the mitochondrial permeability transition (mPT) has been seen as a mechanism of this toxicity. The mitochondrial matrix protein cyclophilin D (CypD) is a key regulator of the mPT, lending itself as a potential target for therapeutic intervention. The overall aim of this research project is to explore the mPT as a potential mediator of drug-induced mitochondrial toxicity in intestine and liver. Small intestinal ulceration is a frequent and serious adverse effect associated with the use of non-steroidal anti-inflammatory drugs. Mitochondria have been implicated in ulcer development. We have shown that inhibition of the mPT pore by pharmacologic blockade of CypD resulted in significant protection from diclofenac injury in cultured enterocytes and a 70% reduction in intestinal ulcers in mice. Furthermore, Ppif-/- (the gene encoding CypD) mice show 80% ulcer reduction
This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, members of which play an important role in protein folding, immunosuppression by cyclosporin A, and infection of HIV-1 virions. [provided by RefSeq, Jul 2008 ...
Redesigning an FKBP-ligand interface to generate chemical dimerizers with novel specificity. Proc Natl Acad Sci U S A (1998) 3.88 The immunophilin FK506-binding protein modulates Ca2+ release channel closure in rat heart. J Physiol (1997) 2.01 Inhibition of human immunodeficiency virus type 1 replication in human cells by Debio-025, a novel cyclophilin binding agent. Antimicrob Agents Chemother (2008) 1.46 Hair growth modulation by topical immunophilin ligands: induction of anagen, inhibition of massive catagen development, and relative protection from chemotherapy-induced alopecia. Am J Pathol (1997) 1.17 Rectification of skeletal muscle ryanodine receptor mediated by FK506 binding protein. Biophys J (1995) 1.10 A role for FKBP52 in Tau protein function. Proc Natl Acad Sci U S A (2010) 1.07 FKBP12 is a critical regulator of the heart rhythm and the cardiac voltage-gated sodium current in mice. Circ Res (2011) 0.99 From nature to the laboratory and into the clinic. Bioorg Med Chem (2008) 0.95 ...
Astrocytes extend highly branched processes that form functionally isolated microdomains, facilitating local homeostasis by redistributing ions, removing neurotransmitters, and releasing factors to influence blood flow and neuronal activity. Microdomains exhibit spontaneous increases in calcium (Ca2+), but the mechanisms and functional significance of this localized signaling are unknown. By developing conditional, membrane-anchored GCaMP3 mice, we found that microdomain activity that occurs in the absence of inositol triphosphate (IP3)-dependent release from endoplasmic reticulum arises through Ca2+ efflux from mitochondria during brief openings of the mitochondrial permeability transition pore. These microdomain Ca2+ transients were facilitated by the production of reactive oxygen species during oxidative phosphorylation and were enhanced by expression of a mutant form of superoxide dismutase 1 (SOD1 G93A) that causes astrocyte dysfunction and neurodegeneration in amyotrophic lateral sclerosis ...
The participation of mitochondria in cellular and neuronal Ca2+ homeostatic networks is now well accepted. Yet, critical tests of specific mitochondrial pathways in neuronal Ca2+ responses have been hampered because the identity of mitochondrial proteins that must be integrated within this dynamic system remain uncertain. One putative pathway for Ca2+ efflux from mitochondria exists through the formation of the permeability transition pore (PTP) that is often associated with cellular and neuronal death. Here, we have evaluated neuronal Ca2+ dynamics and the PTP in single adult neurons in wild-type mice and those missing cyclophilin D (CyPD), a key regulator of the PTP. Using high-resolution time-lapse imaging, we demonstrate that PTP opening only follows simultaneous activation with two physiological stimuli that generate critical threshold levels of cytosolic and mitochondrial Ca2+. Our results are the first to demonstrate CyPD-dependent PTP opening in normal neuronal Ca2+ homeostatic ...
Calcium modulating ligand (CAMLG or CAML), also known as calcium-modulating cyclophilin ligand, is a signalling protein recognized by the TNF receptor TACI. The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin. CAMLG has been shown to interact with TNFRSF13B. GRCh38: Ensembl release 89: ENSG00000164615 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000021501 - Ensembl, May 2017 Human PubMed Reference:. Mouse ...
To characterize the cellular action mechanism of Debio 0507, we compared the major DNA adducts formed by Debio 0507- and oxaliplatin-treated HCT116 human colon carcinoma cells by a combination of inductively coupled plasma mass spectrometry (ICP-MS) and ultraperformance liquid chromatography mass spectrometry (UPLC-MS/MS). HCT116 cells were treated with IC50 doses of Debio 0507 or oxaliplatin for 3 days. Total cellular Pt-DNA adducts were determined by ICP-MS. The DNA was digested, and the major Pt-DNA adducts formed by both drugs were characterized by UPLC/MS/MS essentially as described previously for cisplatin (Baskerville-Abraham et al. in Chem Res Toxicol 22:905-912, 2009). The Pt level/deoxynucleotide was 7.4/104 for DNA from Debio 0507-treated cells and 5.5/104 for oxaliplatin-treated cells following a 3-day treatment at the IC50 for each drug. UPLC-MS/MS in the positive ion mode confirmed the major Pt-DNA adducts formed by both drugs were dach-Pt-d(GpG) (904.2 m/z → 610 m/z and 904.2 m/z →
Recombinant Human Cyclophilin E protein (His tag) is an Escherichia coli Full length protein 2 to 301 aa range, | 95% purity, | 1.000 Eu/µg endotoxin level and validated in SDS-PAGE, MS.
References for Abcams Recombinant human Cyclophilin 40 protein (ab78815). Please let us know if you have used this product in your publication
Rescue and Role of Complex I in Myocardial Ischemic Injury: Members of the Bcl-2 family function at the mitochondrial outer membrane to regulate programmed cell death, or apoptosis. Bax and Bak are multi-domain pro-apoptotic members of the family, but their activity is directly or indirectly regulated by proteins of the BH3-only subfamily (Bcl-2 relatives that only share homology in domain 3). We are interested in Bid, a BH3-only protein that is proteolytically activated during ischemia/reperfusion and targets the mitochondria to initiate apoptosis. Mitochondria can promote necrotic cell death through a second pathway involving a mysterious entity known as the Mitochondrial Permeability Transition Pore, which includes cyclophilin D and other components that are the subject of intense investigation. We hypothesize that the electron transfer Complex I may be a part of the pore and may be regulated by Complex I. We have developed a cell-permeable therapeutic protein that can protect the heart ...
This project will analyze the effects of life-long physical activity on brain function in Alzheimer disease (AD) and aging with special reference to mitochondrial-mediating mechanisms. Behavioral tests, mitochondrial bioenergetics endpoints (oxygen consumption, transmembrane potential) and calcium movements will be performed. Markers of oxidative damage, mitochondrial dynamics, apoptosis and antioxidants, including Bax/Bcl2, SIRT3, p66Shc, MnSOD, aconitase, carbonyls, PGC-1a, Mfn, Drp 1, Fis-1, caspase 3/9 activities, and the mitochondrial permeability transition pore regulators F(1)F(0) ATP-synthase c subunit, ANT and cyclophilin D will be measured. With this project funded by FCT (PTDC/DTP/DES/1246/2012), important contributions to understand the mitochondrial mechanisms associated with the role of exercise to mitigate age- and AD-related brain dysfunction will be provided. Depending on the data obtained, it may be suggested that an active life-style during life course reduces brain ...
70147 (prev NZ4213969); b Timaru 14 May 23; Wanganui Collegiate; farmer - E A Hall, Bellwood, Timaru. NZ Army/TF 13½ mths; RNZAF Levin as Aircrafthand (ADU) 31 Oct 42, Taieri 4 Dec 42, Ashburton 9 Dec 42, Milson 2 Apr 43, remust as Aircrew u/t & ITW 29 Apr 43, remust as Airman Pilot u/t 24 Jun 43, 2EFTS 26 Jun 43, 1SFTS 28 Aug 43, Pilots Badge [wef 1.11.43] & Comm 23 Dec 43, OSI 31 Dec 43, CFS 15 Jan 44, 2EFTS (Tiger Moth) as FI 14 Mar 44, 3EFTS (Tiger Moth) as FI 19 Aug 44 [att CFS for Harvard conv 21 Oct-13 Nov], 2OTU (P-40) 25 Nov 44, CCU (Corsair) 5 Feb 45, 16 Sqn (Corsair) 12 Mar 45, with Sqn to Pacific 1 Apr 45, rtd with Sqn to NZ 23 Jun 45, with Sqn to Pacific 13 Aug 45, rtd with Sqn to NZ 4 Nov 45, 14 Sqn (Corsair) 1 Dec 45, emb with Sqn on light fleet HMS Glory for Japan 8 Mar 46, arr 26 Mar 46, SSComm 1 Apr 46, rtd to NZ 21 Apr 47, SSComm 1 Apr 47, 41 Sqn (Dakota) 30 May 47, Whenuapai for admin duties 6 Jun 47 [Adj from 27 Jun, att Army Sch Trentham 4-10 Feb 48], CFS (various a/c ...
Background:. Since the introduction of cisplatin, carboplatin and oxaliplatin, several new generations of platinum analogues have been developed as candidates for chemotherapy. DEBIO 0507/NC-4016 is a DACH-Platin Polymeric Micelle. The main objectives of this project were to assess pharmacokinetic (PK) profile of DEBIO 0507 and evaluate its efficacy in preclinical studies against a broad spectrum of experimental tumors including syngenic, xenogenic tumor models and a cisplatin-resistant cell line.. Methods:. PK and biodistribution studies were conducted in multiple matrices (blood, tumor, liver, spleen, kidneys and pancreas) collected in mice bearing C38 mouse colon carcinoma and treated with a single IV injection of DEBIO 0507 at 2 mg Pt/kg.. Antitumor activity of DEBIO 0507 intravenously injected every 4, 7 or 14 days at different doses was investigated in C38 mouse colon carcinoma bearing C57BL/6 mice. Antitumor effect of DEBIO 0507 was also assessed in a panel of human tumor xenografted in ...
Activated carbons were characterized texturally and chemically before and after treatment, using surface area determination in the BET model, Boehm titration, TPR, DRX and immersion calorimetry. The adsorption capacity and the kinetics of sulphur compound removal were determined by gas chromatography. It was established that the propanethiol retention capacity is dependent on the number of oxygenated groups generated on the activated carbon surface and that activated carbon modified with CuO at 0.25 M shows the highest retention of propanethiol. Additionally is proposed a mechanism of decomposition of propenothiol with carbon-copper system.
|p|Mextra|sup|®|/sup| Superabsorbent addresses three of the important features of a superabsorbent dressing. High absorption capacity|sup|1,2,|/sup| high retention capacity|sup|1|/sup| and excellent conformability|sup|3|/sup|.  Mex
Mast cells were purified from human skin of healthy donors according to our routine protocol (e.g. PMID: 14634065, 15191551, 15666093, 15675967, 20545757, 24671954, 25725371, 26706922, 28264498, 28845295, 28859248), reaching 98-100% purity, and transfected with siRNA as specified by the Dharmacon; all siRNAs were used at 1 µM. (A) Cellular uptake of siRNA, as measured by fluorescence microscopy using PE-labeled (non-targeting) siRNA (24 h after transfection). (B) and (C) relative gene expression in cells transfected with non-targeting siRNA versus siRNA targeting either GAPDH or Cyclophilin B, measured by RT-qPCR (48 h post-transfection) and normalized to control expression given as 1. Mean ± SEM of n = 6-9 individual experiments. Note that the knockdown was both highly efficient and specific, as only the targeted RNA was downregulated, while the Cyclophilin B-specific siRNA had no impact on GAPDH expression and vice versa. ** p , 0.01; *** p , 0.01; ****p , 0.0001 by Kruskal-Wallis test with ...
NOVEL RNAi THERAPEUTIC FOR TREATMENT OF HEPATITIS C INFECTION - Small interfering RNAs (siRNAs) or small hairpin RNA (shRNAs) and compositions comprising same are provided that specifically target human cyclophilin A (CyPA) to effectively inhibit Hepatitis C(HCV) infection in a cell. Such siRNA and shRNAs may have a length of from about 19 to about 29 contiguous nucleotides corresponding to a specific region of human cyclophilin A (CyPA) cDNA of from about nucleotide 155 to about nucleotide 183 having particular potency against CyPA and HCV. Such siRNA and shRNAs may be formulated as naked compositions or as pharmaceutical compositions. DNA polynucleotides, plasmids, and viral or non-viral vectors are also provided that encode siRNA or shRNA molecules, which may be delivered directly to cells or in combination with known delivery agents, such as lipids, polymers, encapsulated lipid particles, such as liposomes. Methods for treating, managing inhibiting, preventing, etc., HCV infection using such ...
Lausanne, Switzerland (ots/PRNewswire) - - Debio 1450, oral/IV FabI inhibitor active against all Staphylococcus species has received Fast Track designation for ABSSSI (acute...
This work has been made available to the staff and students of the University of Sydney for the purposes of research and study only. It constitutes material that is held by the University for the purposes of reporting for HERDC and the ERA. This work may not be downloaded, copied and distributed to any third party ...
[liste de diffusion pappso-tools] (/mailinglist)Réseaux nationaux en protéomique [Réseau des Plateformes Protéomiques dIle de France] (http://pappso.inra.fr/ppif) [Réseau MassProt’ INRA : huit plateaux de spectrométrie de masse en France] (http://massprot.
ウサギ・ポリクローナル抗体 ab3562 交差種: Ms,Rat,Rb,Chk,Cow,Hu,NHuPrm 適用: WB,IP,IHC-P,IHC-Fr,ICC,Flow Cyt,ICC/IF…Cyclophilin…
As a cyclophilin, PPIB binds the immunosuppressive drug CsA to form a CsA-cyclophilin complex, which then targets calcineurin ... Price ER, Zydowsky LD, Jin MJ, Baker CH, McKeon FD, Walsh CT (Apr 1991). "Human cyclophilin B: a second cyclophilin gene ... Mikol V, Kallen J, Walkinshaw MD (1994). "X-ray structure of a cyclophilin B/cyclosporin complex: comparison with cyclophilin A ... As a cyclophilin, PPIB binds cyclosporin A (CsA) and can be found within in the cell or secreted by the cell. PPIB is the ...
As a cyclophilin, PPIC binds the immunosuppressive drug CsA to form a CsA-cyclophilin complex, which then targets calcineurin ... Depletion of these two cyclophilins lead to hyperoxidation of the ER. In the brain, PPIC complexes with cyclophilin C- ... Like other cyclophilins, PPIC forms a β-barrel structure with a hydrophobic core. This β-barrel is composed of eight anti- ... Yao Q, Li M, Yang H, Chai H, Fisher W, Chen C (Mar 2005). "Roles of cyclophilins in cancers and other organ systems". World ...
As a cyclophilin, PPIF binds the immunosuppressive drug CsA to form a CsA-cyclophilin complex, which then targets calcineurin ... Thus, cyclophilins may function in cardioprotection during ischemia-reperfusion injury. Currently, cyclophilin expression is ... Like other cyclophilins, PPIF forms a β-barrel structure with a hydrophobic core. This β-barrel is composed of eight anti- ... It has also been referred to as, but should not be confused with, cyclophilin D (CypD), which is encoded by the PPID gene. As a ...
As a cyclophilin, PPID binds the immunosuppressive drug CsA to form a CsA-cyclophilin complex, which then targets calcineurin ... Thus, cyclophilins may function in cardioprotection during ischemia-reperfusion injury. Currently, cyclophilin expression is ... Peptidylprolyl isomerase D (cyclophilin D), also known as PPID, is an enzyme which in humans is encoded by the PPID gene on ... Like other cyclophilins, PPID forms a β-barrel structure with a hydrophobic core. This β-barrel is composed of eight anti- ...
As a cyclophilin, PPIE also binds the immunosuppressive drug CsA to form a CsA-cyclophilin complex, which then targets ... Thus, cyclophilins may function in cardioprotection during ischemia-reperfusion injury. Currently, cyclophilin expression is ... Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on ... Wang Z, Liu X, Zhao Z, Xu C, Zhang K, Chen C, Sun L, Gao GF, Ye X, Liu W (2011). "Cyclophilin E functions as a negative ...
Like other cyclophilins, PPIA forms a β-barrel structure with a hydrophobic core. This β-barrel is composed of eight anti- ... Yao Q, Li M, Yang H, Chai H, Fisher W, Chen C (Mar 2005). "Roles of cyclophilins in cancers and other organ systems". World ... Peptidylprolyl isomerase A (PPIA), also known as cyclophilin A (CypA) or rotamase A is an enzyme that in humans is encoded by ... Wei Y, Jinchuan Y, Yi L, Jun W, Zhongqun W, Cuiping W (Jun 2013). "Antiapoptotic and proapoptotic signaling of cyclophilin A in ...
... is a type I integral membrane receptor that has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B ... Yurchenko V, O'Connor M, Dai W, Guo H, Toole B, Sherry B, Bukrinsky M (2001). "CD147 is a signaling receptor for cyclophilin B ... 2002). "Active site residues of cyclophilin A are crucial for its signaling activity via CD147". J. Biol. Chem. 277 (25): 22959 ... 2001). "CD147 is a signaling receptor for cyclophilin B". Biochem. Biophys. Res. Commun. 288 (4): 786-8. doi:10.1006/bbrc. ...
Nestel FP, Colwill K, Harper S, Pawson T, Anderson SK (1997). "RS cyclophilins: identification of an NK-TR1-related cyclophilin ...
Lin CL, Leu S, Lu MC, Ouyang P (2004). "Over-expression of SR-cyclophilin, an interaction partner of nuclear pinin, releases SR ... Nestel FP, Colwill K, Harper S, Pawson T, Anderson SK (Jan 1997). "RS cyclophilins: identification of an NK-TR1-related ... "Entrez Gene: PPIG peptidylprolyl isomerase G (cyclophilin G)". Lin, Chun Lun; Leu Steve; Lu Ming Chu; Ouyang Pin (Aug 2004). " ... 2004). "The human nuclear SRcyp is a cell cycle-regulated cyclophilin". J. Biol. Chem. 279 (21): 22322-30. doi:10.1074/jbc. ...
Horowitz DS, Kobayashi R, Krainer AR (Dec 1997). "A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a ... Horowitz DS, Lee EJ, Mabon SA, Misteli T (Feb 2002). "A cyclophilin functions in pre-mRNA splicing". The EMBO Journal. 21 (3): ... a nuclear cyclophilin" (PDF). The Journal of Biological Chemistry. 275 (11): 7439-42. doi:10.1074/jbc.275.11.7439. PMID ... "Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide". Journal of Molecular ...
FKBP-12 and cyclophilins both share common peptide-prolyl isomerase activity. While the majority of the Peptide bonds within ... These two families are: "cyclosporin-binding cyclophilins (CyPs)" and "FK506-binding proteins (FKBPs)". Recently, a novel group ...
Ferreira PA, Nakayama TA, Pak WL, Travis GH (Oct 1996). "Cyclophilin-related protein RanBP2 acts as chaperone for red/green ... Ferreira PA, Yunfei C, Schick D, Roepman R (Sep 1998). "The cyclophilin-like domain mediates the association of Ran-binding ... Yi H, Friedman JL, Ferreira PA (Nov 2007). "The cyclophilin-like domain of Ran-binding protein-2 modulates selectively the ... Ferreira PA, Hom JT, Pak WL (Sep 1995). "Retina-specifically expressed novel subtypes of bovine cyclophilin". The Journal of ...
This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ... 2005). "Cell surface expression of CD147/EMMPRIN is regulated by cyclophilin 60". J. Biol. Chem. 280 (30): 27866-71. doi: ... "Entrez Gene: PPIL2 peptidylprolyl isomerase (cyclophilin)-like 2". Wang BB, Hayenga KJ, Payan DG, Fisher JM (1996). " ... 2010). "Structural and biochemical characterization of the human cyclophilin family of peptidyl-prolyl isomerases". PLoS Biol. ...
This gene encodes a member of the cyclophilin family. Cyclophilins catalyze the cis-trans isomerization of peptidylprolyl imide ... Huang LL, Zhao XM, Huang CQ, Yu L, Xia ZX (Mar 2005). "Structure of recombinant human cyclophilin J, a novel member of the ... "Entrez Gene: PPIL3 peptidylprolyl isomerase (cyclophilin)-like 3". Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high ... cyclophilin)-like gene (PPIL3) from human fetal brain". Cytogenetics and Cell Genetics. 92 (3-4): 231-6. doi:10.1159/000056909 ...
This gene is a member of the cyclophilin family of peptidylprolyl isomerases (PPIases). The cyclophilins are a highly conserved ... "Entrez Gene: PPIL1 peptidylprolyl isomerase (cyclophilin)-like 1". Tripodis N, Mason R, Humphray SJ, et al. (1999). "Physical ... expression and chromosomal mapping of a novel cyclophilin-related gene (PPIL1) from human fetal brain". Cytogenet Cell Genet. ...
Examples include photoisomerase and immunophilins such as cyclophilin. cis-trans-Isomerases at the US National Library of ...
This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved family, ... "Entrez Gene: PPIL4 peptidylprolyl isomerase (cyclophilin)-like 4". Zeng L, Zhou Z, Xu J, et al. (2002). "Molecular cloning, ... structure and expression of a novel nuclear RNA-binding cyclophilin-like gene (PPIL4) from human fetal brain". Cytogenet. Cell ...
It inhibits cyclophilin A. Alisporivir is not immunosuppressive. It is being researched for potential use in the treatment of ... Alisporivir (INN), or Debio 025, DEB025, (or UNIL-025) is a cyclophilin inhibitor. Its structure is reminiscent of, and ... April 2008). "Inhibition of human immunodeficiency virus type 1 replication in human cells by Debio-025, a novel cyclophilin ... December 2008). "Debio 025, a cyclophilin binding molecule, is highly efficient in clearing HCV replicon containing cells, ...
... which binds cyclophilin. Both the FKBP-tacrolimus complex and the cyclosporin-cyclophilin complex inhibit a phosphatase called ... Along with cyclophilin, FKBPs belong to the immunophilin family. FKBP12 is notable in humans for binding the immunosuppressant ... FKBP, or FK506 binding protein, is a family of proteins that have prolyl isomerase activity and are related to the cyclophilins ... Liu J, Farmer JD, Lane WS, Friedman J, Weissman I, Schreiber SL (August 1991). "Calcineurin is a common target of cyclophilin- ...
When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein ... Bram RJ, Crabtree GR (1994). "Calcium signalling in T cells stimulated by a cyclophilin B-binding protein". Nature. 371 (6495 ... Tovey SC, Bootman MD, Lipp P, Berridge MJ, Bram RJ (2000). "Calcium-modulating cyclophilin ligand desensitizes hormone-evoked ... Guo S, Lopez-Ilasaca M, Dzau VJ (2005). "Identification of calcium-modulating cyclophilin ligand (CAML) as transducer of ...
The Role of Cyclophilin D in learning and memory. Brush, F. Robert (2003). "Selection for Differences in avoidance Learning: ...
Cyp33 is a cyclophilin that has the ability to isomerize H3 proline residues at P16 and P30 positions. Histones H2A and H2B ... Cyclophilin A uses an "electrostatic handle" to pull proline into cis and trans formations. Most of these biological functions ... PPIases are present in three types: cyclophilins, FK507-binding proteins, and the parvulins. PPIase enzymes catalyze the ...
To the end of this, necrosis occurs by physical interaction with the PTP regulator cyclophilin D (CypD). The mitochondrial p53- ... Alam, M.R.; D. Baetz; M. Ovize (2015). "Cyclophilin D and myocardial ischemia-reperfusion injury: a fresh perspective". J Mol ...
CsA binds to cyclophilin D to block the opening of MPTP, and thus decreases the release of protein cytochrome C, which can ... It does this by forming a complex with cyclophilin to block the phosphatase activity of calcineurin, which in turn decreases ... This cyclosporin-cyclophilin complex inhibits calcineurin, which is normally responsible for activating the transcription of ... Handschumacher RE, Harding MW, Rice J, Drugge RJ, Speicher DW (November 1984). "Cyclophilin: a specific cytosolic binding ...
October 2011). "The SARS-coronavirus-host interactome: identification of cyclophilins as target for pan-coronavirus inhibitors ...
Identification of Cyclophilins as Target for Pan-Coronavirus Inhibitors". PLOS Pathogens. 7 (10): e1002331. doi:10.1371/journal ...
... cyclophilin mutant. Cyclosporin A is a chemical inducer of dimerization (CID) of cyclophilin. This first bump-and-hole pair was ... The bumped cyclosporin A was found to interact efficiently with the hole-modified cyclophilin mutant, but not endogenous ... cyclophilin. The orthogonal CID pair was used to inhibit calcineurin-mediated dephosphorylation of nuclear factor of activated ... engineered to improve the binding efficiency between wild type cyclosporin A and cyclophilin, thereby giving more efficient CID ...
Ferreira PA, Nakayama TA, Pak WL, Travis GH (1996). "Cyclophilin-related protein RanBP2 acts as chaperone for red/green opsin ...
Examples comprise fibronectin/long chain cytokines, NEF/SH2, cyclophilin/capsid proteins. Ecology portal McGhee, G.R. (2011) ...
Rotamase The first cyclophilin (Cyp/PPIases: EC 5.2.1.8) was isolated more than three decades ago as an... ... Cyclophilin-like domain (CLD); Immunophilin; Peptidyl prolyl cis trans isomerase; PPIase; ... The single domain cyclophilin has a single cyclophilin-like domain (CLD), whereas multidomain cyclophilins also possess ... Cyclophilin D interacts with Bcl2 and exerts an anti-apoptotic effect. J Biol Chem. 2009;284:9692-9.CrossRefPubMedPubMedCentral ...
Other cyclophilins have similar structures to cyclophilin A. The cyclosporin-cyclophilin A complex inhibits a calcium/ ... Cyclophilin A is a cytosolic and highly abundant protein. The protein belongs to a family of isozymes, including cyclophilins B ... Cyclophilin D (PPIF, note that literature is confusing, the mitochondrial cyclophilin is encoded by the PPIF gene), which is ... Cyclophilin inhibitors, such as cyclosporin, are being developed to treat neurodegenerative diseases. Cyclophilin inhibition ...
The authors suggest that cyclophilin A (CypA), a component of the APOE4-activated pathway, is a potential target for treating ... Using different APOE transgenic mice, including mice with ablation and/or inhibition of cyclophilin A (CypA), here we show that ... Cyclophilin A is a proinflammatory cytokine that activates endothelial cells. Arterioscler. Thromb. Vasc. Biol. 24, 1186-1191 ( ... Cyclophilin A enhances vascular oxidative stress and the development of angiotensin II-induced aortic aneurysms. Nature Med. 15 ...
4 May 2017, by Kathrin Oppermann. The department of Molecular Plant Genetics has published a new research article: "Bioinformatic and expression analysis of the Brassica napus L. cyclophilins" in "Scientific Reports".. The full text article can be found here. ...
PEPTIDYL-PROLYL CIS-TRANS ISOMERASE F, MITOCHONDRIAL1-(4-Aminobenzyl)-3-(2-{(2r)-2-[2-(Methylsulfanyl)phenyl]pyrrolidin-1-Yl}-2-Oxoethyl)urea
cyclophilin-like protein. A. 196. Plasmodium yoelii. Mutation(s): 0 EC: 5.2.1.8. ...
cyclophilin. A. 229. Plasmodium yoelii yoelii. Mutation(s): 0 Gene Names: PY00382. EC: 5.2.1.8. ...
Mouse monoclonal Cyclophilin A antibody. Validated in WB, IP, Flow Cyt, ICC/IF and tested in Human. Cited in 14 publication(s ... Cyclophilin A was immunoprecipitated using 0.5mg Hela whole cell extract, 10µg of Mouse monoclonal to Cyclophilin A and 50µl of ... Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.. Contains 1 PPIase cyclophilin-type domain. ... Primary - Mouse Anti-Cyclophilin A antibody (ab58144) WB, ICC/IF, IP, Flow Cyt ...
Rabbit polyclonal Cyclophilin B antibody. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Rat, Horse, Chicken, Dog, Human ... Human Cyclophilin B peptide (ab16277) at 1 µg/ml. Lane 7 : HeLa whole cell lysate with Human Cyclophilin B peptide (ab16277) at ... Human Cyclophilin B peptide (ab16277) at 1 µg/ml. Lane 9 : Jurkat whole cell lysate with Human Cyclophilin B peptide (ab16277) ... Anti-Cyclophilin B antibody (ab16045) at 0.5 µg/ml + Recombinant Human Cyclophilin B protein (ab88801) at 0.01 µg. Secondary. ...
There are no specific protocols for Recombinant human Cyclophilin F protein (ab79186). Please download our general protocols ...
Cyclophilin-A Human Recombinant, Cyclophilin-B Human Recombinant, Cyclophilin-D Human Recombinant ... Humans are thought to have around 16 of these cyclophilins.. Cyclophilin Function. The Cyclosporin Cyclophilin A complex ... About Cyclophilin:. Cyclophilins are known for their ability to bind ciclosporin, which is used to suppress rejection after a ... Cyclophilin Structure. Cyclophilin A has a beta barrel structure (two alpha helices and a beta sheet). There is usually a ...
... mostly Cyclophilin A (CyPA), in the replication of HCV is supported by a growing body of in vitro and in vivo evidence. CyPA ...
Browse our Cyclophilin C Antibody catalog backed by our Guarantee+. ... Our Cyclophilin C Antibodies can be used in a variety of model species: Human. Use the list below to choose the Cyclophilin C ... Alternate Names for Cyclophilin C Antibodies. anti-Cyclophilin C antibody, anti-PPIC antibody, anti-CYPCMGC3673 antibody, anti- ... Cyclophilin C Antibodies. We offer Cyclophilin C Antibodies for use in common research applications: ELISA, ...
... cyclophilin a include A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype ... Cyclophilin A: A 17-KDa cytoplasmic Peptidylprolyl isomerase involved in immunoregulation. It is a member of the cyclophilin ...
Generation of Rabbit Anti-Cyclophilin and Anti-AGE-Modified Cyclophilin Antisera.. Female New Zealand white rabbits were ... cyclophilin A;. CsA,. cyclosporin A;. PIC,. pre-integration complex;. CA,. capsid antigen;. AGE,. advanced glycosylation ... and 100 nmol of 125I-cyclophilin (either alone, or in combination with a 100-fold excess of cold cyclophilin) was added. After ... A receptor for another member of the cyclophilin family, cyclophilin B (CyPB), which is highly homologous to CyPA, has been ...
The Cyclophilin A-CD147 complex promotes the proliferation and homing of multiple myeloma cells.. Zhu D1, Wang Z2, Zhao JJ1, ... The Cyclophilin A-CD147 complex promotes bone marrow colonization of B-cell malignancies: implications for therapy ... The Cyclophilin A-CD147 complex promotes bone marrow colonization of B-cell malignancies: implications for therapy ... The Cyclophilin A-CD147 complex promotes bone marrow colonization of B-cell malignancies: implications for therapy ...
HIV-1 cyclophilin-binding mutants CA G89V and P90A favored integration in genomic regions with a higher density of ... HIV-1 capsid-cyclophilin interactions determine nuclear import pathway, integration targeting and replication efficiency PLoS ... Here we report that HIV-1 capsid (CA) binds directly to the cyclophilin domain of Nup358/RanBP2. Fusion of the Nup358/RanBP2 ... Our findings link HIV-1 engagement of cyclophilins with both integration targeting and replication efficiency and provide ...
Cyclophilin A is a peptidyl-prolyl isomerase and is widely expressed in a multitude of tissues, but is present in highest ... Cyclophilin A is a small, soluble protein which binds the immunosuppressive drug cyclosporin A. ... The pattern of cyclophilin. A expression is consistent with a role of cyclophilin A in neuronal protein maturation and folding ... Cyclophilin A is a small, soluble protein which binds the immunosuppressive drug cyclosporin A. Cyclophilin A is a peptidyl- ...
13 antibodies to Cyclophilin A and validated for use in 6 applications (Immunohistochemistry,Flow Cytometry,Western Blot, ... cyclophilin; Cyclophilin A; Cyclosporin A-binding protein; CyP A; EC 5.2.1.8; epididymis secretory sperm binding protein Li 69p ... cyclophilin A); PPIase A; rotamase; Rotamase A; SP18; T cell cyclophilin ... Cyclophilin A Antibodies Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major ...
Validated positive control siRNA targeting the Cyclophilin B (PPIB) housekeeping gene in human, mouse, or rat Accell siRNA ... Accell Cyclophilin B Control siRNA Accell Cyclophilin B Control siRNA. Validated Accell positive controls provide a reliable ... Accell Cyclophilin B Control siRNA is modified with patent-pending Accell modification pattern to enable uptake by difficult-to ... Accell Cyclophilin B Control siRNA is validated, highly reliable positive control for delivery and RNAi efficiency. ...
Cyclophilin D (CypD), as the only crucial protein for mitochondrial permeability transition pore (mPTP) induction, facilitates ... Blockade of Cyclophilin D Attenuates Oxidative Stress-Induced Cell Death in Human Dental Pulp Cells,. Oxidative Medicine and ... C. P. Baines, R. A. Kaiser, N. H. Purcell et al., "Loss of cyclophilin D reveals a critical role for mitochondrial permeability ... Cyclophilin D (CypD), as the only crucial protein for mitochondrial permeability transition pore (mPTP) induction, facilitates ...
Cyclophilin A (CypA) is the main member of the immunophilin superfamily that has peptidyl-prolyl cis-trans isomerase activity. ... Keywords: influenza virus; Cyclophilin A; Cyclosporin A; virus-host interaction influenza virus; Cyclophilin A; Cyclosporin A; ... This article belongs to the Special Issue Cyclophilins and Viruses) View Full-Text , Download PDF [274 KB, uploaded 12 May 2015 ... Cyclophilin A (CypA) is the main member of the immunophilin superfamily that has peptidyl-prolyl cis-trans isomerase activity. ...
This review focuses on the role of cyclophilin D (CypD) as a prominent mediator of the mitochondrial permeability transition ...
Although the pore structure is still unresolved, the mechanism through which cyclophilin D (CypD) regulates mPTP opening is the ... Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore. J. ... Cyclophilin D modulates mitochondrial F0F1-ATP synthase by interacting with the lateral stalk of the complex. J. Biol. Chem. ... The prolyl isomerase cyclophilin D (CypD) in the matrix is an important regulator of mPTP opening10,11. CypD was identified as ...
A Cyclophilin Function in Hsp90-Dependent Signal Transduction. By Andrea A. Duina, Hui-Chen Jane Chang, James A. Marsh, Susan ... A Cyclophilin Function in Hsp90-Dependent Signal Transduction. By Andrea A. Duina, Hui-Chen Jane Chang, James A. Marsh, Susan ... A Cyclophilin Function in Hsp90-Dependent Signal Transduction Message Subject. (Your Name) has forwarded a page to you from ... Cpr6 and Cpr7, the Saccharomyces cerevisiae homologs of cyclophilin-40 (CyP-40), were shown to form complexes with Hsp90, a ...
Cyclophilin: distribution and variant properties in normal and neoplastic tissues.. A J Koletsky, M W Harding, R E ... Cyclophilin: distribution and variant properties in normal and neoplastic tissues.. A J Koletsky, M W Harding, R E ... Cyclophilin: distribution and variant properties in normal and neoplastic tissues.. A J Koletsky, M W Harding and R E ... Cyclophilin: distribution and variant properties in normal and neoplastic tissues. Message Subject (Your Name) has forwarded a ...
Transfection of cyclophilin-D (CyP-D, CyP3) into COS-7 cell lines demonstrated no changes in any tested parameters. Both ... No difference was observed in activity or expression in cyclophilin or expression of the MCC in any of the models. It was noted ... Role of cyclophilins and heat shock proteins in myocardial ischaemia/reperfusion injury ... The role of cyclophilins, heat shock proteins (HSP), and the mitochondrial chaperone complex (MCC), was studied in this ...
Activation of a Phytopathogenic Bacterial Effector Protein by a Eukaryotic Cyclophilin. By Gitta Coaker, Arnold Falick, Brian ... Activation of a Phytopathogenic Bacterial Effector Protein by a Eukaryotic Cyclophilin. By Gitta Coaker, Arnold Falick, Brian ... AvrRpt2 is delivered into the plant cell, where it is activated by a eukaryotic factor that we identify as cyclophilin. This ... Activation of a Phytopathogenic Bacterial Effector Protein by a Eukaryotic Cyclophilin Message Subject. (Your Name) has ...
Cyclophilin (CyP) is one of the enzymes that act as peptidylprolyl cis-trans isomerases (EC 5.2.1.8). The cDNA and an ... Cyclophilin (CyP) is one of the enzymes that act as peptidylprolyl cis-trans isomerases (EC 5.2.1.8). The cDNA and an ...
These findings establish cyclophilin C as an ER cyclophilin, demonstrate the novel involvement of cyclophilins B and C in ER ... cyclophilin B , peptidylprolyl isomerase B , peptidylprolyl isomerase B (cyclophilin B) , PPIase B , peptidyl-prolyl cis-trans ... anti-Peptidylprolyl Isomerase A (Cyclophilin A)-Like 4G Antikörper * anti-Peptidylprolyl Isomerase A (Cyclophilin A)-Like 4A ... Zusätzlich bieten wir Ihnen Peptidylprolyl Isomerase B (Cyclophilin B) Kits (62) und Peptidylprolyl Isomerase B (Cyclophilin B ...
  • Using different APOE transgenic mice, including mice with ablation and/or inhibition of cyclophilin A (CypA), here we show that expression of APOE4 and lack of murine Apoe, but not APOE2 and APOE3, leads to BBB breakdown by activating a proinflammatory CypA-nuclear factor-κB-matrix-metalloproteinase-9 pathway in pericytes. (nature.com)
  • More recently, host-derived cyclophilin A (CyPA) has been shown to be incorporated into HIV-1 virions and its incorporation essential for viral infectivity. (pnas.org)
  • The finding that cyclophilin A (CyPA), a cellular protein that binds cyclosporin A (CsA) and possesses peptidyl-prolyl cis-trans isomerase activity ( 1 , 2 ), is specifically incorporated into HIV-1 virions through interactions with the Gag polyprotein ( 3 , 4 ) and is required for effective infection ( 5 - 8 ) raised a number of interesting questions about the biology of this phenomenon. (pnas.org)
  • Because cyclophilins can control protein folding by catalyzing peptidyl-prolyl isomerization ( 9 , 10 ), it has been proposed that CyPA may modify the conformation of capsid antigen (CA), a Gag polyprotein cleavage product that binds CyPA ( 11 ), thus promoting uncoating of the virion and formation of the pre-integration complex (PIC) ( 6 ). (pnas.org)
  • Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. (thermofisher.com)
  • CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells. (thermofisher.com)
  • Cyclophilin A (CypA) is the main member of the immunophilin superfamily that has peptidyl-prolyl cis-trans isomerase activity. (mdpi.com)
  • New World monkeys of the genus Aotus synthesize a fusion protein (AoT5Cyp) containing tripartite motif-containing 5 (TRIM5) and cyclophilin A (CypA) that potently blocks HIV-1 infection. (sigmaaldrich.com)
  • Cyclophilin A (CyPA, encoded by Ppia) is a ubiquitously expressed protein secreted in response to pro-inflammatory stimuli. (ahajournals.org)
  • Moreover, en face staining of aortic tissues showed that VCAM-1 expression was significantly reduced in mice lacking CyPA. (ahajournals.org)
  • Cyclophilin A (CYPA) is the first member of the cyclophilins to be identified in mammals. (biovendor.com)
  • CypA (cyclophilin A) is a ubiquitous and highly conserved protein with peptidyl prolyl isomerase activity. (ahajournals.org)
  • Viral incorporation of cyclophilin A (CyPA) during the assembly of human immunodeficiency virus type-1 (HIV-1) is crucial for efficient viral replication. (uzh.ch)
  • Peptides corresponding to these regions showed higher affinities (Kd approximately 0.3 microM) for both CyPA and cyclophilin B than the best peptide derived from the Gly-Pro90 site ( approximately 8 microM) and thus revealed new sequence motifs flanking Gly-Pro that are important for tight interaction of peptide ligands with cyclophilins. (uzh.ch)
  • Consequently, CyPA and cyclophilin B bound with much higher affinities ( approximately 0.6 and 0.25 microM) to the new, i.e. maturation-generated sites. (uzh.ch)
  • Cyclophilin A (CypA) is a peptidyl-prolyl isomerase that binds to the capsid protein (CA) of human immunodeficiency virus type 1 (HIV-1) and by doing so facilitates HIV-1 replication. (pubmedcentralcanada.ca)
  • Cyclophilin A (CypA) is the most abundant member of a ubiquitous family of peptidyl-prolyl isomerases and binds to the capsid (CA) domain of human immunodeficiency virus type 1 (HIV-1) and SIVcpz Gag proteins ( 26 ). (pubmedcentralcanada.ca)
  • OBJECTIVE Cyclophilin A (CyPA) is an abundant intracellular protein that is considered to be the main target of the immunosuppressive drug cyclosporine A. We and others showed that CyPA is secreted from smooth muscle cells and macrophages in response to oxidative stress and lipopolysaccharide, suggesting a role for CyPA in inflammation. (semanticscholar.org)
  • The capsid (CA) protein of HIV-1 binds with high affinity to the host protein cyclophilin A (CypA). (biomedcentral.com)
  • This binding positively affects some early stage of the viral life-cycle because prevention of binding either by drugs that occupy that active site of cyclophilin A, by mutation in HIV-1 CA, or RNAi that knocks down intracellular CypA level diminishes viral infectivity. (biomedcentral.com)
  • Cyclophilin A (CyPA) is a potent chemokine, which can directly induce leukocyte chemotaxis and contribute to the pathogenesis of inflammation-mediated diseases. (readbyqxmd.com)
  • 2005). Furthermore, it has been shown that severe acute respiratory syndrome (SARS)-N protein interacts with cellular proteins, including cyclophilin A (CypA), heterogenous nuclear ribonucleoprotein (hnRNP) A1, human ubiquitin-conjugating enzyme, cyclin dependent kinase (CDK)-cyclin complex protein, Ikappaßalpha (IkBα), cytochrome (Cyt) P450 etc. (uwc.ac.za)
  • Peptidyl-prolyl cis-trans isomerase A, also known as PPIase A, Rotamase A, Cyclophilin A, Cyclosporin A-binding protein, PPIA and CYPA, is a cytoplasm protein which belongs to the cyclophilin-type PPIase family and PPIase A subfamily. (sinobiological.com)
  • In this study, we found that expression of cyclophilin A (CypA) was significantly upregulated in Spodoptera litura hemocytes at 6‐day post‐parasitization. (usda.gov)
  • The structure of the ternary complex between the drug cyclosporin A (CsA), human cyclophilin A (CypA) and human calcineurin [37]. (biomedcentral.com)
  • The H87Q mutation is located in the cyclophilin A (CypA) binding loop and has a prevalence of 21% in HIV-1 sequences registered in HIV database. (biomedcentral.com)
  • BACKGROUND: The human peptidyl-prolyl isomerase Cyclophilin A (CypA) binds HIV-1 capsid (CA) and influences early steps in the HIV-1 replication cycle. (umassmed.edu)
  • Three independent studies recently demonstrated that HCV highly relies on cyclophilin A (CypA) to replicate in human hepatocytes. (grantome.com)
  • PPIB is the second of 18 cyclophilins to be identified in humans, after CypA. (wikipedia.org)
  • Cyclophilin: a specific cytosolic binding protein for cyclosporin A. Science. (springer.com)
  • The cytosolic concentration, Mr, and isoforms of cyclophilin (CyP), a specific cytosolic binding protein for cyclosporin A (CsA), were determined in normal and neoplastic human tissues as well as tissues from species of diverse phylogeny. (jimmunol.org)
  • Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. (prospecbio.com)
  • Two other novel findings arose in the course of the study, namely a 54 kD proline-rich cyclophilin D binding protein (identified by pull-downs and mass spectrometry) and phosphorylation of a (non- identified) 17 kD protein in cyclophilin D overexpressing mitochondria. (ucl.ac.uk)
  • Cyclophilin B (CyPB) is a cyclosporin A (CsA)binding protein mainly located in intracellular vesicles and secreted in biological fluids. (cnrs-orleans.fr)
  • The two classical immunophilin families, found essentially in all living cells, are: cyclophilin (CYN) and FK506-binding protein (FKBP). (usda.gov)
  • The PPIase family is further divided into three structurally distinct subfamilies: cyclophilin (CyP), FK506-binding protein (FKBP), and parvulin (Pvn). (wikipedia.org)
  • Cyclophilins comprise a ubiquitous set of proteins which is present across diverse genera of organisms. (springer.com)
  • Cyclophilins (CYPs) are a family of proteins named after their ability to bind to ciclosporin (cyclosporin A), an immunosuppressant which is usually used to suppress rejection after internal organ transplants. (wikipedia.org)
  • Cyclophilins have peptidyl-prolyl cis-trans isomerase activity, and these proteins are collectively known as immunophilins. (prospecbio.com)
  • It is a member of the cyclophilin family of proteins that binds to Cyclosporine . (jove.com)
  • Cyclophilins are a family of proteins that bind cyclosporin A (CsA) and possess peptidyl-prolyl cis-trans isomerase activity. (pnas.org)
  • Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). (thermofisher.com)
  • The role of cyclophilins, heat shock proteins (HSP), and the mitochondrial chaperone complex (MCC), was studied in this pathological condition. (bl.uk)
  • Additionally we are shipping Peptidylprolyl Isomerase B (Cyclophilin B) Antibodies (152) and Peptidylprolyl Isomerase B (Cyclophilin B) Proteins (31) and many more products for this protein. (antibodies-online.com)
  • Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins engineered from human components. (sigmaaldrich.com)
  • and structural proteins ( cyclophilin , actin, and chitinase). (thefreedictionary.com)
  • Human have a total of 16 cyclophilin proteins. (biovendor.com)
  • It was first identified in a yeast two-hybrid screen for cyclophilin B-interacting proteins ( 9 ). (jimmunol.org)
  • Cyclophilins are ubiquitous cellular proteins with peptidyl-prolyl cis-trans isomerase enzymatic activity, which are involved in a variety of functions related to cell metabolism, energy homeostasis, and exhibit enhanced expression in inflammation or malignancy. (biovendor.com)
  • The three PPIase subfamilies, cyclophilins, FKBPs (FK506 binding proteins) and parvulins form a group by their enzymatic activity despite lack of sequence similarity between the subfamilies. (diva-portal.org)
  • Cyclophilins play roles in folding and chaperoning of cellular proteins and are the major receptors for the immunosuppressive drug cyclosporin A (CsA). (tcd.ie)
  • We, and others, have previously characterized two CsA-binding cyclophilins (PfCYP19A and PfCYP19B) but a family of genes encoding uncharacterized cyclophilins/cyclophilin-like proteins is also seen in the P. falciparum genome (Figure 1). (tcd.ie)
  • Cyclophilins (CyPs) are a family of proteins found in organisms ranging from prokaryotes to humans. (sinobiological.com)
  • PPIA / Cyclophilin A accelerate the folding of proteins. (sinobiological.com)
  • PPIA / Cyclophilin A can interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. (sinobiological.com)
  • Proteins with prolyl isomerase activity include cyclophilins, FKBPs, and parvulin. (cambridgemedchemconsulting.com)
  • GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Mouse Anti Human Cyclophilin-B Cyclophilin-B. (antibody-antibodies.com)
  • Cyclophilins are highly conserved proteins and the target of the drug cyclosporin but their physiological functions remain enigmatic. (grantome.com)
  • Additionally, the sheer number of the cyclophilin family members found within the spliceosomal machinery and their unique distribution throughout splicing complexes indicate that these proteins are likely to be crucial for proper splicing activity. (grantome.com)
  • In cardiac myogenic cells, cyclophilins have been observed to be activated by heat shock and hypoxia-reoxygenation as well as complex with heat shock proteins. (wikipedia.org)
  • Cyclophilin D (CypD), as the only crucial protein for mitochondrial permeability transition pore (mPTP) induction, facilitates the opening of mPTP and causes mitochondrial dysfunction, leading to cell death. (hindawi.com)
  • This review focuses on the role of cyclophilin D (CypD) as a prominent mediator of the mitochondrial permeability transition pore (MPTP) and subsequent effects on cardiovascular physiology and pathology. (go.jp)
  • Although the pore structure is still unresolved, the mechanism through which cyclophilin D (CypD) regulates mPTP opening is the subject of intensive studies. (nature.com)
  • Cyclophilin D (CypD) is a mitochondrial matrix protein implicated in cell death, but a potential role in bioenergetics is not understood. (lu.se)
  • Opening of the pore is regulated by the mitochondrial peptidyl prolyl-cis, trans-isomerase cyclophilin D (CYPD). (frontiersin.org)
  • Here, we show that deletion or reduction in the levels of cyclophilin D (CypD, also called Ppif), a mitochondrial matrix peptidyl prolyl isomerase and apoptosis regulator, results in increased cell proliferation and enhanced cell migration and invasion. (lu.se)
  • The matrix protein cyclophilin D (CypD) is the best known regulator of PTP opening. (ovid.com)
  • Cyclosporin A (CsA) inhibits the opening of the mitochondrial permeability transition pore (MPTP) by interacting with cyclophilin D (CypD) and ameliorates neuronal cell death in the central nervous system against ischemic injury. (arvojournals.org)
  • Under these conditions, mitochondrially-localized STAT3 also became competent to bind to cyclophilin D (CypD). (nottingham.ac.uk)
  • RATIONALE: Mice lacking cyclophilin D (CypD), a mitochondrial chaperone protein, have altered cardiac metabolism. (elsevier.com)
  • Cyclophilin D (CypD) is an important component in mitochondrial-dependent tubular cell death in acute kidney injury. (monash.edu)
  • Each Cyclophilin C Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Choose from our Cyclophilin C polyclonal antibodies and browse our Cyclophilin C monoclonal antibody catalog. (novusbio.com)
  • Fluorescent double-labelling experiments on hippocampal cell cultures revealed that all cells labelled with the cyclophilin A antibody also showed staining for the neuron-specific marker for microtubule-associated protein (MAP)2, and could, therefore, be identified as neurons. (nih.gov)
  • View detailed Cyclophilin G antibody specifications by linking to the specific product blocks. (scbt.com)
  • There are currently no images for Cyclophilin C Antibody (NBP1-97946AF700). (novusbio.com)
  • Western Blot (WB) analysis of 1) Jurkat, 2)293T, 3)Rat Liver Tissue, 4)3T3 with Cyclophilin B Mouse Monoclonal Antibody diluted at 1:2000. (genetex.com)
  • Cyclophilin D antibody LS-C163901 is an unconjugated rabbit polyclonal antibody to Cyclophilin D (PPID) (aa85-115) from human. (lsbio.com)
  • Mouse Anti Human Cyclophilin-B Cyclophilin-B antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Mouse Anti Human Cyclophilin-B Cyclophilin-B. (antibody-antibodies.com)
  • Validated positive control siRNA targeting the Cyclophilin B (PPIB) housekeeping gene in human, mouse, or rat. (horizondiscovery.com)
  • Also known as peptidylprolyl isomerase B (PPIB), cyclophilin B is abundantly expressed in most mammalian cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability. (horizondiscovery.com)
  • A missense mutation in cyclophilin B (PPIB) is reported as a novel, causal candidate gene for hereditary equine regional dermal asthenia (HERDA). (antikoerper-online.de)
  • LS-F43019 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Chicken PPIB / Cyclophilin B. It is based upon a Custom assay principle and can be used to detect levels of PPIB / Cyclophilin B as low as 18.75 nanograms per millilter. (lsbio.com)
  • The Staphylococcus aureus cyclophilin PpiB is an intracellular peptidyl prolyl cis/trans isomerase (PPIase) that has previously been shown to contribute to secreted nuclease and hemolytic activity. (asm.org)
  • PpiB is a functional PPIase belonging to the cyclophilin family, and it assists in the refolding of Nuc in vitro . (asm.org)
  • A validated positive silencing control targeting the Cyclophilin B (PPIB) gene in human, mouse, or rat cell lines. (gelifesciences.com)
  • Also known as peptidylprolyl isomerase B (PPIB), cyclophilin B is associated with the secretory pathway. (gelifesciences.com)
  • Gentaur Molecular :Fivephoton \ Mouse Cyclophilin B (CYPB, PPIB, Peptidylprolyl Isomerase B) ELISA KITS. (antibody-antibodies.com)
  • Product Detail : mCYPB-ELISA(2X96T) Mouse Cyclophilin B (CYPB, PPIB, Peptidylprolyl Isomerase B) ELISA KITS. (antibody-antibodies.com)
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  • Like other cyclophilins, PPIB forms a β-barrel structure with a hydrophobic core. (wikipedia.org)
  • As a cyclophilin, PPIB binds cyclosporin A (CsA) and can be found within in the cell or secreted by the cell. (wikipedia.org)
  • As a cyclophilin, PPIB binds the immunosuppressive drug CsA to form a CsA-cyclophilin complex, which then targets calcineurin to inhibit the signaling pathway for T-cell activation. (wikipedia.org)
  • Recombinant full length protein corresponding to Human Cyclophilin A aa 1-165. (abcam.com)
  • Cyclophilin B Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (34-216 a.a) and having a molecular mass of 22.7kDa. (prospecbio.com)
  • Full length, recombinant human Cyclophilin 40 expressed in E. coli. (genetex.com)
  • Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques. (antibodies-online.com)
  • However neither cyclophilin D (overexpression) nor cyclophilin A (addition of recombinant enzyme) stimulated cytochrome c release. (ucl.ac.uk)
  • Other cyclophilins have similar structures to cyclophilin A. The cyclosporin-cyclophilin A complex inhibits a calcium/calmodulin-dependent phosphatase, calcineurin, the inhibition of which is thought to suppress organ rejection by halting the production of the pro-inflammatory molecules TNF alpha and interleukin 2. (wikipedia.org)
  • Cyclophilin D is thought to regulate the opening of the pore because cyclosporin A, which binds to CyP-D, inhibits the pore opening. (wikipedia.org)
  • Cyclophilin inhibitors, such as cyclosporin, are being developed to treat neurodegenerative diseases. (wikipedia.org)
  • Cyclophilin A is a small, soluble protein which binds the immunosuppressive drug cyclosporin A. Cyclophilin A is a peptidyl-prolyl isomerase and is widely expressed in a multitude of tissues, but is present in highest concentration in the brain. (nih.gov)
  • TLP20 was inhibited by cyclosporin A and mass spectrometric sequencing of tryptic peptides confirmed its classification as a cyclophilin. (diva-portal.org)
  • Cyclophilin binds to the immunosuppressant Cyclosporin A (Cat. (emdmillipore.com)
  • Cyclophilin is characterized by its binding to the immunosuppressant cyclosporin A. This complex then binds calcineurin (PP2B) and inhibits its phosphatase activity. (emdmillipore.com)
  • Cyclophilin (CyP) is one of the enzymes that act as peptidylprolyl cis-trans isomerases (EC 5.2.1.8). (apsnet.org)
  • Zusätzlich bieten wir Ihnen Peptidylprolyl Isomerase B (Cyclophilin B) Kits (62) und Peptidylprolyl Isomerase B (Cyclophilin B) Proteine (31) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • Cyclophilin is the trivial name for peptidylprolyl isomerase, EC 5.2.1.8 (peptidylproline cis-trans-isomerase). (thefreedictionary.com)
  • Full length Clone DNA of Rat peptidylprolyl isomerase A (cyclophilin A) with N terminal HA tag. (sinobiological.com)
  • Cyclophilin D (CYP D), is a member of a family of highly homologous peptidylprolyl cis-trans isomerases (PPIases) that interconvert the cis and trans isomers of peptide bonds with the amino acid proline. (cambridgemedchemconsulting.com)
  • De plus, nous expédions Peptidylprolyl Isomerase E (Cyclophilin E) Protéines (24) et beaucoup plus de produits pour cette protéine. (anticorps-enligne.fr)
  • This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. (acris-antibodies.com)
  • We offer Cyclophilin C Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. (novusbio.com)
  • Our Cyclophilin C Antibodies can be used in a variety of model species: Human. (novusbio.com)
  • Santa Cruz Biotechnology, Inc. offers a broad range of Cyclophilin G antibodies. (scbt.com)
  • Select Cyclophilin G antibodies from monoclonal antibodies listed below. (scbt.com)
  • Select appropriate Cyclophilin G antibodies for your research by isotype, epitope, applications and species reactivity. (scbt.com)
  • Simon Kerr, chief executive officer, Cypralis, said, "Cyprolides are a new class of fully synthetic cyclophilin inhibitors that are potent inhibitors of cyclophilin D and protect against opening of the mitochondrial permeability transition pore. (thefreedictionary.com)
  • Cyclophilin D inhibitors for treating human conditions such as ischemia/reperfusion injury, trauma and neurodegenerative diseases. (thefreedictionary.com)
  • This study shows that the company's cyclophilin inhibitors can be further developed towards the goal of offering mitochondrial myopathy patients a novel treatment option. (thefreedictionary.com)
  • Inhibitors of Cyclophilin D have been postulated as potential drugs for a variety of therapeutic targets including anti-viral activity DOI , neurodegenration DOI , Cancer DOI etc. (cambridgemedchemconsulting.com)
  • At the recent Macrocycles 2018 meeting Vicky Steadman described the identification and optimisation of potent and orally available selective Cyclophilin inhibitors, more details have just been published J Med Chem paper DOI . (cambridgemedchemconsulting.com)
  • A growing body of evidence suggests that cyclophilins (Cyps) are important for the HCV life cycle, suggesting that Cyp inhibitors (CsA, Debio 025, NIM811 or SCY- 635), by acting on intracellular Cyps, block HCV replication. (grantome.com)
  • By demonstrating that cyclophilin (Cyp) inhibitors have potent efficacy against HCV, our recent clinical study opens a new line of opportunity to eradicate this prime human threat. (grantome.com)
  • Fusion of the Nup358/RanBP2 cyclophilin (Cyp) domain to the tripartite motif of TRIM5 created a novel inhibitor of HIV-1 replication, consistent with an interaction in vivo. (nih.gov)
  • Nevertheless, CSA, a potent inhibitor of cyclophilins and the permeability transition, did inhibit cytochrome c release. (ucl.ac.uk)
  • The Cyclophilin A Inhibitor, also referenced under CAS 1186372-20-2, controls the biological activity of Cyclophilin A. This small molecule/inhibitor is primarily used for Inflammation/Immunology applications. (emdmillipore.com)
  • Cyclophilin inhibitor 1 is a potent and orally bioavailable cyclophilin A inhibitor, with a K d of 5 nM, shows effective anti- HCV activity, with an EC 50 of 98 nM for HCV 2a. (medchemexpress.com)
  • Cyclophilin inhibitor 1 (Compound 3) inhibits HCV 2a activity, with an EC 50 of 98 nM [1] . (medchemexpress.com)
  • Discovery of a Potent and Orally Bioavailable Cyclophilin Inhibitor Derived from the Sanglifehrin Macrocycle. (medchemexpress.com)
  • Regulation of the tyrosine kinase Itk by the peptidyl-prolyl isomerase cyclophilin A. Proc Natl Acad Sci USA. (springer.com)
  • The cellular peptidyl-prolyl isomerase cyclophilin A is incorporated into human immunodeficiency virus type 1 virions via contacts with the proline-rich domain of the Gag polyprotein. (asm.org)
  • Cyclophilins (Cyp) are a family of cellular enzymes possessing peptidyl-prolyl isomerase activity which catalyze the cis-trans interconversion of proline-containing peptide bonds. (auburn.edu)
  • 2008). CV: Prolyl isomerase cyclophilin A regulation of Janus-activated kinase 2 and the progression of human breast cancer. (core.ac.uk)
  • Belongs to the cyclophilin-type PPIase family. (abcam.com)
  • Contains 1 PPIase cyclophilin-type domain. (abcam.com)
  • The most active PPIase in the spinach lumen was identified as the cyclophilin TLP20. (diva-portal.org)
  • In addition to PPIase and protein chaperone activities, cyclophilins function in mitochondrial metabolism, apoptosis, immunological response, inflammation, and cell growth and proliferation. (wikipedia.org)
  • Cyclophilin inhibition may also be a therapy for liver diseases. (wikipedia.org)
  • The Cyclophilin A-CD147 complex promotes the proliferation and homing of multiple myeloma cells. (nih.gov)
  • The first cyclophilin (Cyp/PPIases: EC 5.2.1.8) was isolated more than three decades ago as an intracellular receptor of the immunosuppressive drug cyclosporine A (CsA) from the bovine thymocyctes (Handschumacher et al. (springer.com)
  • Cyclophilin A, in particular, is the major intracellular receptor for CsA. (biovendor.com)
  • Coimmunoprecipitation and intracellular binding studies suggested that LKT binds to the mitochondrial matrix protein cyclophilin D. We also demonstrated that LKT mobilizes the vesicle scission protein dynamin-2 from mitochondria to the cell membrane. (asm.org)
  • 2001). Human cyclophilin 40 is a heat shock protein that exhibits altered intracellular localization following heat shock. (core.ac.uk)
  • 2006). Knuckey NW: Evidence that intracellular cyclophilin A and cyclophilin A/CD147 receptormediated ERK1/2 signalling can protect neurons against in vitro oxidative and ischemic injury. (core.ac.uk)
  • Genes encoding similar putative thylakoid cyclophilins with a unique insert of three amino acids NPV in their N-termini were found in chromosome 5 of both Arabidopsis and rice. (diva-portal.org)
  • 2009). CV: Expression of cyclophilin B is associated progression and regulation with malignant of genes implicated in pathogenesis of breast cancer. (core.ac.uk)
  • Cyclophilin A is a cytosolic and highly abundant protein. (wikipedia.org)
  • As potential targets of CSA, the isolated mitochondria contained both cyclophilin D (mitochondrial matrix) and residual cyclophilin A (a cytosolic enzyme). (ucl.ac.uk)
  • An additional function of the rough endoplasmic reticulum protein complex prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B: the CXXXC motif reveals disulfide isomerase activity in vitro. (antikoerper-online.de)
  • Notably, treatment with antioxidants rescues mitochondrial damage markers in fibroblasts from patients with X-linked adrenoleukodystrophy, including cyclophilin D oxidative modifications, and reverses cyclophilin D induction in vitro and in vivo. (sigmaaldrich.com)
  • Ray, Rialon-Guevara, Veras, Sullenger, White: Comparing human pancreatic cell secretomes by in vitro aptamer selection identifies cyclophilin B as a candidate pancreatic cancer biomarker. (antibodies-online.com)
  • Neither mutation alters Gag's cyclophilin A-binding properties in vitro, and cyclophilin A incorporation into drug-resistant virions is effectively disrupted by cyclosporine A, indicating that the drug-resistant mutants do not require virion-associated cyclophilin A to initiate infection. (asm.org)
  • Our findings link HIV-1 engagement of cyclophilins with both integration targeting and replication efficiency and provide insight into the conservation of viral cyclophilin recruitment. (nih.gov)
  • Cyclophilin A: A Key Factor in Virus Replication and Potential Target for Anti-viral Therapy. (semanticscholar.org)
  • De Iaco, Alberto and Luban, Jeremy, "Cyclophilin A promotes HIV-1 reverse transcription but its effect on transduction correlates best with its effect on nuclear entry of viral cDNA" (2014). (umassmed.edu)
  • The isomerase active site of cyclophilin a is critical for hepatitis C virus replication. (thefreedictionary.com)
  • Structurally, CypB shows a high degree of homology with other members of the cyclophilin family in its core β-barrel/isomerase region, which contains a surface hydrophobic pocket that constitutes the proline binding motif. (biovendor.com)
  • Data indicate that the mutation in cyclophilin B (CypB) locally rearranges the flexible N-terminal end of the polypeptide chain and also makes it more rigid. (antikoerper-online.de)
  • Both N- and C- termini of CypB differ significantly from other cyclophilin family members. (biovendor.com)
  • Contrasting effects of electroconvulsive shock on mRNAs encoding the high affinity kainate receptor subunits (KA1 and KA2) and cyclophilin in the rat. (ox.ac.uk)
  • Accell Cyclophilin B Control siRNA is validated, highly reliable positive control for delivery and RNAi efficiency. (horizondiscovery.com)
  • Accell Cyclophilin B Control siRNA is modified with patent-pending Accell modification pattern to enable uptake by difficult-to-transfect cells. (horizondiscovery.com)
  • siGENOME Cyclophilin B Control siRNA is a validated positive control, guaranteed to silence cyclophilin B in human, mouse, or rat cells. (gelifesciences.com)
  • siSTABLE Non-Targeting siRNA #1 (D-001700-01, Lanes 1 and 4), siGENOME Cyclophilin B siRNA (D-001136-01, Lanes 2 and 5), and siSTABLE Cyclophilin B siRNA (D-001710-02, Lanes 3 and 6). (horizondiscovery.com)
  • Cyclophilin-A HumanRecombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 185 amino acids (1-165 a.a.) and having a molecular mass of 20 kDa. (neobiolab.com)
  • Recombiant, human cyclophilin A expressed in E. coli . (emdmillipore.com)
  • I have already begun to use this assay to test for splicing activity in the presence of the cyclophilin PPIE, and can show that indeed this protein is necessary for proper splicing function. (grantome.com)
  • Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Cyclophilin B. (abcam.com)
  • Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).Can be blocked with Human Cyclophilin B peptide (ab16277) . (abcam.com)
  • Cyclophilins are known for their ability to bind ciclosporin, which is used to suppress rejection after a human has an internal organ transplant. (prospecbio.com)
  • Cyclosporine A-resistant human immunodeficiency virus type 1 mutants demonstrate that Gag encodes the functional target of cyclophilin A. (asm.org)
  • 1987), Complementary DNA for human T-cell cyclophilin. (sinobiological.com)
  • 1990), Characterization of the human cyclophilin gene and of related processed pseudogenes.Eur. (sinobiological.com)
  • Mouse Anti Human Cyclophilin-B Cyclophilin-B Human samples 80 % of the research is conducted on human samples. (antibody-antibodies.com)
  • A synthetic peptide corresponding to a sequence at the C-terminus of human Cyclophilin B(176-196aa MEVVRKVESTKTDSRDKPLKD), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. (bosterbio.com)
  • 2006). Cyclophilin A is overexpressed in human pancreatic cancer cells and stimulates cell proliferation through CD147. (core.ac.uk)
  • 2005). Effect of cyclophilin A on gene expression in human pancreatic cancer cells. (core.ac.uk)
  • Based on the finding that several nuclear cyclophilins are enriched in purified human splicing complexes, it is likely that the targets of spliceosomal cyclophilins will provide a great deal of information concerning cyclophilin:substrate specificity. (grantome.com)
  • A new family of cyclophilins with an RNA recognition motif that interact with members of the trx (Montrer GSR Anticorps )/ MLL (Montrer MLL Anticorps ) protein family in Drosophila and human cells. (anticorps-enligne.fr)
  • Detects cyclophilin 40 (CyP 40) from Human and Rat tissues and cells. (abcam.co.jp)
  • Synthetic peptide corresponding to Human Cyclophilin 40 aa 356-370. (abcam.co.jp)
  • Cyclophilins also have varying degrees of affinity for the immunosuppressive drug Cyclosporine A (CsA), a cyclic 11-amino-acid peptide produced by fungus Tolypocladium infantum. (biovendor.com)
  • Cyclophilins and FKBPs, collectively called immunophilins, were originally discovered as the targets of the immunosuppressive drugs cyclosporine A and FK506, respectively. (diva-portal.org)
  • That Gag's functional dependence on cyclophilin A can be differentiated genetically from its ability to bind cyclophilin A is further demonstrated by the rescue of a mutation precluding cyclophilin A packaging by a mutation conferring cyclosporine A resistance. (asm.org)
  • Loss of cyclophilin D reveals a critical role for mitochondrial permeability transition in cell death. (springer.com)
  • Cyclophilin D (PPIF, note that literature is confusing, the mitochondrial cyclophilin is encoded by the PPIF gene), which is located in the matrix of mitochondria, is only a modulatory, but may or may not be a structural component of the mitochondrial permeability transition pore. (wikipedia.org)
  • However, mitochondria obtained from the cysts of Artemia franciscana, do not exhibit the mitochondrial permeability transition pore Overexpression of Cyclophilin A has been linked to poor response to inflammatory diseases, the progression or metastasis of cancer, and aging. (wikipedia.org)
  • Properties of the permeability transition pore in mitochondria devoid of Cyclophilin D". J. Biol. (wikipedia.org)
  • Among the mitochondrial permeability transition pore components, cyclophilin D is the most studied and has been found increased under pathological conditions. (sigmaaldrich.com)
  • However, using mitochondrially entrapped fluorophores and agents that promote the transition (Ca2+, overexpression of mitochondrial cyclophilin D), BID-induced cytochrome c release was in fact found to be independent of permeability transition. (ucl.ac.uk)
  • 2007). PC: Thrombin-cleaved COOH-terminal osteopontin peptide binds with cyclophilin C to CD147 in murine breast cancer cells. (core.ac.uk)
  • Cyclophilin A is also known to be recruited by the Gag polyprotein during HIV-1 virus infection, and its incorporation into new virus particles is essential for HIV-1 infectivity. (wikipedia.org)
  • in parallel with the disruption of cyclophilin A incorporation into virions, there is a linear reduction in the initiation of reverse transcription after infection of a T cell. (asm.org)
  • Cyclophilins are distributed widely among different organisms and are proposed drug targets for a number of diseases including HIV and hepatitis C infection and ischemia. (tcd.ie)
  • donovanichaperone cyclophilin 40 (LdCyP40) for infection. (ovid.com)
  • A expression is consistent with a role of cyclophilin A in neuronal protein maturation and folding in vivo. (nih.gov)
  • Moreover, we show increased expression levels of cyclophilin D in the affected zones of brains in patients with adrenomyeloneuropathy, in spinal cord of a mouse model of X-linked adrenoleukodystrophy (Abcd1-null mice) and in fibroblasts from patients with X-linked adrenoleukodystrophy. (sigmaaldrich.com)
  • We also measured the expression of cyclophilin mRNA, and found it to be reduced in all hippocampal subfields, and in the parietal cortex, after a single ECS. (ox.ac.uk)
  • 1999). Expression of the estrogen receptor-associated immunophilins, cyclophilin 40 and FKBP52, in breast cancer. (core.ac.uk)
  • Allain F, Vanpouille C, Carpentier M, Slomianny MC, Durieux S, Spik G. Interaction with glycosaminoglycans is required for cyclophilin B to trigger integrin-mediated adhesion of peripheral blood T lymphocytes to extracellular matrix. (springer.com)
  • Moreover, DB25, a CSA analogue that inhibits the catalytic activity of both cyclophilins, but does not allow interaction with downstream targets such as calcineurin, was ineffective, and instead had a stimulatory effect on cytochrome c release. (ucl.ac.uk)
  • Here we report two new interaction sites between cyclophilins and p24. (uzh.ch)
  • Interaction with glycosaminoglycans is required for cyclophilin B to trigger integrin-mediated adhesion of peripheral blood T lymphocytes to extracellular matrix. (semanticscholar.org)
  • Cyclophilin eluted as a sharp peak with an apparent molecular mass of 19 kDa. (pnas.org)
  • 2003). Protein expression profiling identifies macrophage migration inhibitory factor and cyclophilin a as potential molecular targets in non-small cell lung cancer. (core.ac.uk)
  • 1999). Itoh K: A cyclophilin B gene encodes antigenic epitopes recognized by HLA-A24-restricted and tumorspecific CTLs. (core.ac.uk)
  • Oxidative stress modulates mitochondrial failure and cyclophilin D function in X-linked adrenoleukodystrophy. (sigmaaldrich.com)
  • These experiments demonstrate that, in addition to its ability to package cyclophilin A into virions, gag encodes the functional target of cyclophilin A. (asm.org)
  • 1Z81: Crystal Structure of cyclophilin from Plasmodium yoelii. (rcsb.org)
  • Cyclophilins contribute to Stat3 signaling and survival of multiple myeloma cells. (springer.com)
  • Cyclophilin B activity regulated secretion and activity of ADAMTS13 (show ADAMTS13 ELISA Kits ). (antibodies-online.com)
  • PPIA / Cyclophilin A is secreted by vascular smooth muscle cells in response to inflammatory stimuli, and could thus contribute to atherosclerosis. (sinobiological.com)
  • Cyclophilins are involved in a myriad of physiological cellular processes, such as protein trafficking and maturation, receptor complex stabilization, apoptosis, receptor. (springer.com)
  • Cyclophilin A as a downstream effector of PI3K/Akt signalling pathway in multiple myeloma cells. (springer.com)
  • The results of these structure/function studies of spliceosomal cyclophilins will expand our understanding of splicing mechanism to include the roles of cyclophilin specific protein:protein interactions and proline isomerization within the spliceosome. (grantome.com)