Cycloheximide
Protein Synthesis Inhibitors
Compounds which inhibit the synthesis of proteins. They are usually ANTI-BACTERIAL AGENTS or toxins. Mechanism of the action of inhibition includes the interruption of peptide-chain elongation, the blocking the A site of ribosomes, the misreading of the genetic code or the prevention of the attachment of oligosaccharide side chains to glycoproteins.
Dactinomycin
A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)
Puromycin
Protein Biosynthesis
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells, Cultured
Emetine
The principal alkaloid of ipecac, from the ground roots of Uragoga (or Cephaelis) ipecacuanha or U. acuminata, of the Rubiaceae. It is used as an amebicide in many different preparations and may cause serious cardiac, hepatic, or renal damage and violent diarrhea and vomiting. Emetine inhibits protein synthesis in EUKARYOTIC CELLS but not PROKARYOTIC CELLS.
Transcription, Genetic
Enzyme Induction
Anisomycin
Pactamycin
Gene Expression Regulation
Depression, Chemical
The decrease in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
Tumor Cells, Cultured
Blotting, Northern
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Chloramphenicol
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Tetradecanoylphorbol Acetate
Fibroblasts
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Dose-Response Relationship, Drug
Colchicine
Amanitins
Cyclic peptides extracted from carpophores of various mushroom species. They are potent inhibitors of RNA polymerases in most eukaryotic species, blocking the production of mRNA and protein synthesis. These peptides are important in the study of transcription. Alpha-amanitin is the main toxin from the species Amanitia phalloides, poisonous if ingested by humans or animals.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
Liver
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Bucladesine
Ornithine Decarboxylase
Peptide Biosynthesis
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Interleukin-1
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Biological Transport
Carbon Isotopes
Tumor Necrosis Factor-alpha
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Cyclic AMP
Deoxyadenosines
Polyribosomes
A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
L Cells (Cell Line)
Stimulation, Chemical
The increase in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
Half-Life
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Rats, Inbred Strains
Enzyme Activation
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Antimetabolites
Aminoisobutyric Acids
Gene Expression
Enzyme Inhibitors
Ribosomes
Culture Techniques
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Bcl-2 and Bcl-XL serve an anti-inflammatory function in endothelial cells through inhibition of NF-kappaB. (1/5972)
To maintain the integrity of the vascular barrier, endothelial cells (EC) are resistant to cell death. The molecular basis of this resistance may be explained by the function of antiapoptotic genes such as bcl family members. Overexpression of Bcl-2 or Bcl-XL protects EC from tumor necrosis factor (TNF)-mediated apoptosis. In addition, Bcl-2 or Bcl-XL inhibits activation of NF-kappaB and thus upregulation of proinflammatory genes. Bcl-2-mediated inhibition of NF-kappaB in EC occurs upstream of IkappaBalpha degradation without affecting p65-mediated transactivation. Overexpression of bcl genes in EC does not affect other transcription factors. Using deletion mutants of Bcl-2, the NF-kappaB inhibitory function of Bcl-2 was mapped to bcl homology domains BH2 and BH4, whereas all BH domains were required for the antiapoptotic function. These data suggest that Bcl-2 and Bcl-XL belong to a cytoprotective response that counteracts proapoptotic and proinflammatory insults and restores the physiological anti-inflammatory phenotype to the EC. By inhibiting NF-kappaB without sensitizing the cells (as with IkappaBalpha) to TNF-mediated apoptosis, Bcl-2 and Bcl-XL are prime candidates for genetic engineering of EC in pathological conditions where EC loss and unfettered activation are undesirable. (+info)NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae. (2/5972)
A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. Molecular genetic analysis of NMD3 revealed that it is an essential gene required for stable 60S ribosomal subunits. Cells bearing a temperature-sensitive allele of NMD3 had decreased levels of 60S subunits at the nonpermissive temperature which resulted in the formation of half-mer polysomes. Pulse-chase analysis of rRNA biogenesis indicated that 25S rRNA was made and processed with kinetics similar to wild-type kinetics. However, the mature RNA was rapidly degraded, with a half-life of 4 min. Nmd3p fractionated as a cytoplasmic protein and sedimented in the position of free 60S subunits in sucrose gradients. These results suggest that Nmd3p is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. Putative orthologs of Nmd3p exist in Drosophila, in nematodes, and in archaebacteria but not in eubacteria. The Nmd3 protein sequence does not contain readily recognizable motifs of known function. However, these proteins all have an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization. (+info)delta-Aminolevulinate synthetases in the liver cytosol fraction and mitochondria of mice treated with allylisopropylacetamide and 3,5-dicarbethoxyl-1,4-dihydrocollidine. (3/5972)
Hepatic delta-aminolevulinate (ALA) synthetase was induced in mice by the administration of allylisopropylacetamide (AIA) and 3,5-dicarbethoxy-1,4-dihydrocollidine (DDC). In both cases, a significant amount of ALA synthetase accumulated in the liver cytosol fraction as well as in the mitochondria. The apparent molecular weight of the cytosol ALA synthetase was estimated to be 320,000 by gel filtration, but when the cytosol ALA synthetase was subjected to sucrose density gradient centrifugation, it showed a molecular weight of 110,000. In the mitochondria, there were two different sizes of ALA synthetase with molecular weights of 150,000 and 110,000, respectively; the larger enzyme was predominant in DDC-treated mice, whereas in AIA-treated mice and normal mice the enzyme existed mostly in the smaller form. When hemin was injected into mice pretreated with DDC, the molecular size of the mitochondrial ALA synthetase changed from 150,000 to 110,000. The half-life of ALA synthetase in the liver cytosol fraction was about 30 min in both the AIA-treated and DDC-treated mice. The half-life of the mitochondrial ALA synthetase in AIA-treated mice and normal mice was about 60 min, but in DDC-treated mice the half-life was as long as 150 min. The data suggest that the cytosol ALA synthetase of mouse liver is a protein complex with properties very similar to those of the cytosol ALA synthetase of rat liver, which has been shown to be composed of the enzyme active protein and two catalytically inactive binding proteins, and that ALA synthetase may be transferred from the liver cytosol fraction to the mitochondria with a size of about 150,000 daltons, followed by its conversion to enzyme with a molecular weight of 110,000 within the mitochondria. The process of intramitochondrial enzyme degradation seems to be affected in DDC-treated animals. (+info)Microvessels from Alzheimer's disease brains kill neurons in vitro. (4/5972)
Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease. (+info)Expression of thrombospondin-1 in ischemia-induced retinal neovascularization. (5/5972)
Thrombospondin-1 is an extracellular matrix protein that inhibits endothelial cell proliferation, migration, and angiogenesis. This study was performed to investigate the role of thrombospondin-1 in ischemic retinal neovascularization. In a murine model of retinal neovascularization, thrombospondin-1 mRNA was increased from postnatal day 13 (P13), with a threefold peak response observed on P15, corresponding to the time of development of retinal neovascularization. Prominent expression of thrombospondin-1 was observed in neovascular cells, specifically, cells adjacent to the area of nonperfusion. It has been suggested that vascular endothelial growth factor (VEGF) plays a major role in ischemia-induced retinal neovascularization of this model, so we studied the effects of VEGF on thrombospondin-1 expression. In bovine retinal microcapillary endothelial cells, VEGF induced a biphasic response of thrombospondin-1 expression; VEGF decreased thrombospondin-1 mRNA 0.41-fold after 4 hours, whereas it increased, with a threefold peak response, after 24 hours. VEGF-induced endothelial cell proliferation was completely inhibited by exogenous thrombospondin-1 and increased by 37.5% with anti-thrombospondin-1 antibody. The present findings suggest that, in the ischemic retina, retinal neovascular cells increase thrombospondin-1 expression, and VEGF may stimulate endogenous thrombospondin-1 induction, which inhibits endothelial cell growth. VEGF-mediated thrombospondin-1 induction in ischemia-induced angiogenesis may be a negative feedback mechanism. (+info)5'-Nucleotidase activity of mouse peritoneal macrophages. II. Cellular distribution and effects of endocytosis. (6/5972)
The diazonium salt of sulfanilic acid (DASA) can inactivate about 80% of the total 5'-nucleotidase of viable macrophages. The remaining 20% can be inactivated if the cells are first lysed in detergent, and presumably represents an intracellular pool of 5'-nucleotidase. The bulk of this pool may represent cytoplasmic vesicles derived from plasma membrane by endocytosis. This internal compartment is expanded up to threefold immediately after the cells have ingested a large latex load. This is consistent with previous observations on the internalization of 5'-nucleotidase in latex phagosomes. In latex-filled cells this intracellular pool of enzyme is inactivated over a few hours, and the cells then slowly increase their enzyme activity to nearly normal levels. However, 24 h after latex ingestion the metabolism of 5'-nucleotidase in these recovered cells is abnormal, as the rate of enzyme degradation is about twice the normal rate, and the DASA-insensitive enzyme pool in these cells is strikingly diminished. This may reflect effects of the accumulated indigestible particles on the fate of incoming pinocytic vesicles or on newly synthesized plasma membrane precursor. Another endocytic stimulus, concanavalin A, also reduces the total cell 5'-nucleotidase activity. This effect, which is time and temperature dependent, can be prevented by the competitive sugar alpha-methyl mannose. The concanavalin A inhibition can be reversed in the absence of new protein synthesis or in cells cultivated in serum-free conditions. It is not known whether the effect of concanavalin A on 5'-nucleotidase depends upon the interiorizaiton of plasma membrane or is strictly associated with events at the cell surface. (+info)CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo. (7/5972)
cAMP activated insertion of the cystic fibrosis transmembrane conductance regulator (CFTR) channels from endosomes to the apical plasma membrane has been hypothesized to regulate surface expression and CFTR function although the physiologic relevance of this remains unclear. We previously identified a subpopulation of small intestinal villus epithelial cells or CFTR high expressor (CHE) cells possessing very high levels of apical membrane CFTR in association with a prominent subapical vesicular pool of CFTR. We have examined the subcellular redistribution of CFTR in duodenal CHE cells in vivo in response to the cAMP activated secretagogue vasoactive intestinal peptide (VIP). Using anti-CFTR antibodies against the C terminus of rodent CFTR and indirect immunofluorescence, we show by quantitative confocal microscopy that CFTR rapidly redistributes from the cytoplasm to the apical surface upon cAMP stimulation by VIP and returns to the cytoplasm upon removal of VIP stimulation of intracellular cAMP levels. Using ultrastructural and confocal immunofluorescence examination in the presence or absence of cycloheximide, we also show that redistribution was not dependent on new protein synthesis, changes in endocytosis, or rearrangement of the apical cytoskeleton. These observations suggest that physiologic cAMP activated apical membrane insertion and recycling of CFTR channels in normal CFTR expressing epithelia contributes to the in vivo regulation of CFTR mediated anion transport. (+info)Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (8/5972)
A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided. (+info)
The effects of anisomycin (a protein synthesis inhibitor) on spatial
learning and memory in CA1 region of rats hippocampus
HTLV receptor is an early T-cell activation marker whose expression requires de novo protein synthesis | Blood | American...
Prevalent deficiency in tumor cells of cycloheximide-induced cycle arr by E E. Medrano and A B. Pardee
Export Data and Price of Cycloheximide | www.eximpulse.com
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McClean: Cycloheximide Stock Solution - OpenWetWare
Protective effect of cycloheximide upon protein synthesis by l5178y ce by J E. Fuhr, M Overton et al.
cycloheximide | VWR
Gene expression
Effect of protein synthesis inhibitors on the hormonal induction of alanine and aspartate aminotransferases in the liver of...
Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities | Journal of Cell Science
Plouhinec JL et al. (2014),
Pax3 and Zic1 trigger the early neural crest ge... -
Xenbase Paper
Hypoxic neuronal necrosis: Protein synthesis-independent activation of a cell death program | Proceedings of the National...
Search Results for oxytocin | joe
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Search Results | Journal of Cell Biology | Rockefeller University Press
Cycloheximide - Creative Peptides
A) Western blot demonstrating cycloheximide effects of | Open-i
Protein Synthesis Inhibitors | Flashcards
TNFα-induced lysosomal membrane permeability is downstream of MOMP and triggered by caspase-mediated NDUFS1 cleavage and ROS...
SynPharm: A New Annexe to the Guide to PHARMACOLOGY | guidetopharmacology blog
Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation...
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Cycloheximide - Wikipedia
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The Effect of Cycloheximide on the Replication of Measles Virus | Microbiology Society
Constitutive Nuclear Factor κB Activity Is Required for Survival of Activated B Cell-like Diffuse Large B Cell Lymphoma Cells ...
Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response...
SR9243 | CAS#1613028-81-1 | LXR inverse agonist | MedKoo
Cycloheximide, Crystalline | Krackeler Scientific, Inc.
Anisomycin | Abcam
Anisomycin | Purity |98% | CAS 22862-76-6 | Alomone Labs
Personal induction planner
N-methyl-D-aspartate receptor-mediated neuroprotection in cerebellar granule cells requires new RNA and protein synthesis | PNAS
Download Free Protein Synthesis Like A Banana Split Answer Key, High quality vector files
Protein Synthesis Worksheet. Worksheets. Doriandnimo Thousands of Printable Activities
Novel cycloheximide derivatives with antimicrobial activity against Legionella pneumophila
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Impact of Micro and Macroporous TFF Membranes on Product Sieving and Chromatography Loading for Perfusion Cell Culture | Aspen...
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A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina | Development
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chloramphenicol (thing) by witnie - Everything2.com
Transcriptional regulation of prolactin receptor gene expression by sodium butyrate in MCF-7 human breast cancer cells | Garvan...
Biochemical characterization of spiromesifen and spirotetramat as lipid synthesis inhibitors on cotton leaf worm, Spodoptera...
Apoptosis induced by mild hyperthermia in human and murine tumour cell lines: A study using electron microscopy and DNA gel...
北京大学医学部机构知识库([email protected]): Cycloheximide and actinomycin D delay death and affect bcl-2, bax, and ice gene expression in...
kbrin.a-bldg.louisville.edu
A Physical Model Illustrating Protein Synthesis on the Ribosome | The American Biology Teacher | University of California Press
Thymidine (DThyd) | DNA/RNA Synthesis Inhibitor | MedChemExpress
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Protein synthesis: steps and process
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Cycloheximide
... was reported in 1946 by Alma Joslyn Whiffen-Barksdale at the Upjohn Company. Cycloheximide can be used as an ... Cycloheximide has also been used to make isolation of bacteria from environmental samples easier. Cycloheximide has been used ... Cycloheximide is a naturally occurring fungicide produced by the bacterium Streptomyces griseus. Cycloheximide exerts its ... Treating cells with cycloheximide in a time-course experiment followed by western blotting of the cell lysates for the protein ...
Cycloheximide chase
Disadvantages Some disadvantages to conducting cycloheximide chase assays include the toxic nature of cycloheximide. When used ... Cycloheximide chases can be used with a wide variety of model systems and can be implemented to study almost any protein ... For this reason, cycloheximide chases do not typically last for more than 12 hours. This presents a limitation if the turnover ... Cycloheximide is relatively inexpensive compound compared to other drugs and it is effective when used in low doses for short ...
Glutarimide
Sisler, Hugh D.; Siegel, Malcolm R. (1967). "Cycloheximide and Other Glutarimide Antibiotics". Mechanism of Action. pp. 283-307 ... and cycloheximide, a potent inhibitor of protein synthesis. Glutarimide - Sigma-Aldrich Paris, G.; Berlinguet, L.; Gaudry, R.; ...
Chlamydosauromyces
It is also cycloheximide resistant. C. punctatus can reproduce both in sexual and asexual forms. The teleomorph phase is ...
Lomentospora prolificans
This species is sensitive to cycloheximide. As this species may be slow to emerge from clinical materials, specimens in which ...
Acetoxycycloheximide
Cycloheximide Mahy, Brian W J (2001). A dictionary of virology (3. ed.). San Diego, Calif. [u.a.]: Academic Press. pp. 2. ISBN ... It can be considered as the acetylated analogue of cycloheximide. It is a potent protein synthesis inhibitor in animal cells ...
Naganishia adeliensis
... also grows on 0.01% cycloheximide. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV, Lumbsch HT, ...
Streptomyces albulus
... produces acetoxycycloheximide, aciphenol, albanoursin and cycloheximide. List of Streptomyces species LPSN ...
Cyanidioschyzon
Yagisawa F; Nishida K; Okano Y; Minoda A; Tanaka K; Kuroiwa T (2004). "Isolation of cycloheximide-resistant mutants of ...
Geomyces pannorum
... is resistant to the antifungal agent cycloheximide. However the growth of this species is inhibited by ...
Eukaryotic ribosome
Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin". Nat Chem Biol. 6 (3): 209-217. doi: ... One toxic inhibitor of eukaryotic translation elongation is the glutarimide antibiotic cycloheximide (CHX), which has been co- ...
Lactimidomycin
March 2010). "Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin". Nature Chemical Biology. 6 ...
Translation (biology)
These include anisomycin, cycloheximide, chloramphenicol, tetracycline, streptomycin, erythromycin, and puromycin. Prokaryotic ...
Bacterivore
Adding cycloheximide can inhibit the growth of bacterivores without affecting some bacterial species, but it has also been ... Tremaine, Sarah C. Mills, Aaron L. (1987). "Inadequacy of the Eucaryote Inhibitor Cycloheximide in Studies of Protozoan Grazing ... "Cycloheximide: Aspects of Inhibition of Protein Synthesis in Mammalian Cells". Science. 146 (3650): 1474-1476. Bibcode:1964Sci ...
Alma Joslyn Whiffen-Barksdale
List of mycologists Cycloheximide Achlya bisexualis "Alma Whiffen Barksdale (1916-1981)". Smithsonian Institution Archives. ... While there, she discovered the chemical cycloheximide (trade name Actidione), an anti-fungal and anti-bacterial agent produced ... Alma Joslyn Whiffen-Barksdale (October 25, 1916 - July 5, 1981) was an American mycologist who discovered cycloheximide. She ...
Mucor racemosus
It has been shown to adapt to famous antibiotics like cycloheximide, trichodermin and amphotericin B. Cells adapted to ... Shearer G, Jr; Sypherd, PS (March 1988). "Cycloheximide efflux in antibiotic-adapted cells of the fungus Mucor racemosus". ... cycloheximide particularly have been observed to be 40-times more resistant than non-adapted cells to the drug. These adapted ...
Ribosome profiling
This is commonly performed with cycloheximide but other chemicals can be employed. It is also possible to forgo translation ... RNA-ribosome complexes Cycloheximide Nucleases Phenol/Chloroform Reverse transcriptase dNTPs Sequencing method-cDNA library. ... The other elongating regions can be detected by adding antibiotics like cycloheximide that inhibit translocation, ...
Harold M. Weintraub
"Conservative segregation of parental histones during replication in the presence of cycloheximide". Proc Natl Acad Sci USA. 76 ...
C-5 sterol desaturase
Abe Fumiyoshi; Hiraki Toshiki (2009). "Mechanistic role of ergosterol in membrane rigidity and cycloheximide resistance in ...
Microsporum nanum
Like many other dermatophytes, M. nanum is tolerant of the antifungal agent cycloheximide. In addition, M. nanum also exhibits ...
Parthenogenesis
Treatment with cycloheximide, a non-specific protein synthesis inhibitor, enhances parthenote development in swine presumably ... "Effects of Cycloheximide on Parthenogenetic Development of Pig Oocytes Activated by Ultrasound Treatment". Journal of ...
Adrenal ferredoxin
1988). "Human adrenodoxin: cloning of three cDNAs and cycloheximide enhancement in JEG-3 cells". J. Biol. Chem. 263 (7): 3240-4 ...
Cln3
Finally, it was also shown that this delay occurred even with short pulses of cycloheximide, confirming that an unstable ... It was later shown that treatment with the protein synthesis inhibitor cycloheximide delayed Start in yeast, indicating that ... "Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide". Experimental Cell Research ...
Cyclin D1
Baliga BS, Pronczuk AW, Munro HN (August 1969). "Mechanism of cycloheximide inhibition of protein synthesis in a cell-free ... Obrig TG, Culp WJ, McKeehan WL, Hardesty B (January 1971). "The mechanism by which cycloheximide and related glutarimide ...
Trichophyton rubrum
... rubrum cultures can be isolated on both cycloheximide-containing media and cycloheximide-free media. The latter are ... In primary outgrowth on Sabouraud dextrose agar with cycloheximide and antibacterials, contaminating organisms may cause ...
Trichophyton tonsurans
CLS growth is essentially unaffected by antibiotic treatment with chloramphenicol, as well as by cycloheximide. After 5 days of ...
Cunninghamella bertholletiae
Unlike in the case of dimorphic pathogenic fungi, growth of C. bertholletiae is inhibited by cycloheximide. As a member of the ...
Trichosporon asteroides
... grows in a range of media including in the presence of the antifungal agent, cycloheximide. Colonies of ...
Neothramycin
MC916-C4, a cycloheximide-producing strain, by Umezawa et al. Subsequent testing has shown its capabilities as an anticancer ...
Anisomycin
Cycloheximide Anisomycin Archived January 19, 2012, at the Wayback Machine from Sigma Aldrich Grollman, Arthur P. (1967). " ...
Roscovitine a particular inhibitor of cyclin dependent kinases and cycloheximide a protein synthesis
Author: 'Hou, N. N.' / Subject: Glycine max / Subject term: cycloheximide - PubAg Search Results
IMSEAR at SEARO: Effect of cycloheximide on intestinal transport of methionine & glycine.
Sabouraud Dextrose Agar w Chloramphenicol w Cycloheximide - ALPHACHEM
Cycloheximide (100 mg/ml, DMSO) - 5x1ml - LabCloud Plus+
Legionnaires Disease: Laboratory Guidance for Processing Samples | CDC
Results for 'Diabetes' | Abcam: antibodies, proteins, kits...
Arsenic activates EGFR pathway signaling in the lung
Cycloheximide and disulfoton are positive in the photoclastogencity assay but do not absorb UV irradiation: another example of...
Cycloheximide and disulfoton are positive in the photoclastogencity assay but do not absorb UV irradiation: another example of ... Follow-up testing showed that neither cycloheximide nor disulfoton was positive in the 3T3-NRU assay, the standard in vitro ... The results showed that cycloheximide, disulfoton and tetracycline were clastogenic in CHO cells following UVR exposure (solar- ... These data suggest that both cycloheximide and disulfoton are pseudophotoclastogens, like zinc oxide. Together, these data ...
PP3150 - Dermatophyte Test Medium (DTM) with Chloramphenicol & Cyclohexamide (Actidione) - E & O Laboratories Ltd
PP3150 - Dermatophyte Test Medium (DTM) with Chloramphenicol & Cyclohexamide (Actidione). Home/Pre Poured Petri Dishes/Clinical ... Cyclohexamide (Actidione) is also added to suppress the growth of most yeasts and saprophytic fungi. Dermatophytes appear as ... Dermatophyte Test Medium with Chloramphenicol & Cyclohexamide (Actidione). This is a selective medium for the isolation of ... Send Enquiry for PP3150 - Dermatophyte Test Medium (DTM) with Chloramphenicol & Cyclohexamide (Actidione) ...
Pharmacological induction of Hsp70 protects apoptosis-prone cells from doxorubicin: comparison with caspase-inhibitor- and...
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...
Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening...
Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening ... Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening ... Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening ... 53Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening ...
CYCLOHEXIMIDE MODIFICATION OF THE EFFECTS OF INSULIN ON CALCIUM TRANSPORT IN CARDIAC SARCOLEMMA - статья | ИСТИНА -...
CYCLOHEXIMIDE MODIFICATION OF THE EFFECTS OF INSULIN ON CALCIUM TRANSPORT IN CARDIAC SARCOLEMMAстатья ... Possible mechanisms of modifying effect of cycloheximide on the function of insulin-dependent regulatory system in the ... Pretreatment of myocardial preparation with cycloheximide in low concentrations completely blocks the inhibitory insulin effect ...
British Library EThOS: Investigating temperature signalling pathways in Arabidopsis thaliana using small molecules
In addition, the translation inhibitor cycloheximide was identified as a repressor of heat-inducible genes and an activator of ... heat stress ; HSP70 ; chemical genomics ; Syngenta ; cold shock ; calcium signalling ; translation inhibitor ; cycloheximide ; ... or cycloheximide-induced gene expression, thus implicating translational machinery in the regulation of temperature signalling ...
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Onychomycosis: Practice Essentials, Background, Pathophysiology
Axonal Protein Synthesis and Degradation Are Necessary for Efficient Growth Cone Regeneration | Journal of Neuroscience
... cycloheximide, 91 ± 6%; lactacystin, 89 ± 5%) and retinal (cycloheximide, 88 ± 7%; lactacystin, 90 ± 4%) cultures, indicating ... a, Time-lapse images showing the typical responses of axons to axotomy and the effects of cycloheximide and lactacystin. Axons ... Effects of protein synthesis inhibitor cycloheximide (25 μm) and proteasome-dependent degradative inhibitor lactacystin (10 μm ... Cultures were treated with cycloheximide and lactacystin, and axonal elongation was monitored. The proportion of axons whose ...
Pellino 1 Communicates Intercellular Signaling in Chronic Skin Inflammatory Microenvironment | bioRxiv
G. Representative cycloheximide chase assay of IFR4 turnover in HaCaT cells transfected with control vector (pMyc) or a vector ... MG132 and cycloheximide, A.G Scientific. All antibodies used in this study are listed in Appendix Table S1. ... Whole cell lysates were harvested at various time points after exposure to cycloheximide and IRF4 levels were determined by ... we transfected HaCaT cells with plasmid encoding Myc-Peli1 in the presence of protein synthesis inhibitor cycloheximide (CHX) ( ...
JCI -
Production of Antibodies Specific for Fc, Fab′, and Streptokinase-Streptodornase In Vitro by Peripheral Blood Cells from...
Recommendations for the Prevention and Management of Chlamydia trachomatis Infections, 1993
Phosphorylated ATF1 at Thr184 promotes metastasis and regulates MMP2 expression in gastric cancer | Journal of Translational...
... luciferase reporter assay and cycloheximide experiment. The Cancer Genome Atlas (TCGA) data were used to analyze the expression ... Cycloheximide treatment. 50 μg/ml cycloheximide was applied to the MGC803 cells transfected with Vector, ATF1-WT, ATF1-T184D ... D Cycloheximide experiment showed effect of p-ATF1-T184 on the stability of ATF1 protein in MGC803 ... luciferase reporter assay and cycloheximide experiment. The Cancer Genome Atlas (TCGA) data were used to analyze the expression ...
The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane | eLife
Oxoid - Product Detail
STAA Selective Supplement SR0151 contains cycloheximide and thallous acatate and is toxic if swallowed, inhaled or if in ... STAA Medium is made selective by the inclusion of streptomycin sulphate, thallous acetate and actidione (cycloheximide). STA ... The incorporation of cycloheximide serves to further inhibit yeasts and filamentous fungi. ... Medium is modified from the original formulation by removal of the toxic selective agent cycloheximide as recommended by the ...
Cooling shock induction of protein synthesis by larvae of Galleria mellonella (L.)(Insecta: Lepidoptera) | Masarykova univerzita
NIOSHTIC-2 Search Results - Basic View
ChloramphenicolActidioneActinomycinTranslation inhibitorProtein synthesisGlycinePolymyxinAssaySelectiveReceptorCellsGeneRegulationDrugsMediumEffectCultureSuggestMethodResistance to cycloheximideInhibitionInhibitor of protein synthesisTranslation elongationMcCoyProteinsInhibitsAntifungalChloramphenicolNeuronsSabouraudStabilizationAntibioticCellsBacterialStimulationAccumulationGeneSupplementInfectionTreatmentCandidaEffectsInjectionPresenceResponsesMediumAdministrationPlasmaMethodTime
Chloramphenicol2
- We isolated P. orbiculare on a peptone-glucose-yeast extract medium containing chloramphenicol and cycloheximide, and overlaid with olive oil. (who.int)
- Culturing is performed routinely with Sabouraud agar and the addition of cycloheximide and chloramphenicol. (medscape.com)
Actidione2
- Cyclohexamide (Actidione) is also added to suppress the growth of most yeasts and saprophytic fungi. (eolabs.com)
- STAA Medium is made selective by the inclusion of streptomycin sulphate, thallous acetate and actidione (cycloheximide). (oxoid.com)
Actinomycin2
- The selection of apoptosis inducers include actinomycin D , camptothecin , cycloheximide , dexamethasone , doxorubicin and etoposide . (gbiosciences.com)
- Elevations of δ-aminolaevulic acid synthetase activity caused by dehydroepiandrosterone could be abolished by the antibiotics cycloheximide and actinomycin D. (portlandpress.com)
Translation inhibitor2
- In addition, the translation inhibitor cycloheximide was identified as a repressor of heat-inducible genes and an activator of early cold-inducible genes. (bl.uk)
- By using a proteasome inhibitor MG132 and a translation inhibitor cyclohexamide, we showed that CDCA reduced HIF-1α protein by decreasing its translation but not by enhancing its degradation. (elsevier.com)
Protein synthesis1
- Application of protein synthesis inhibitors (cycloheximide and anisomycin) to cut axons, including axons whose cell bodies were removed, or proteasome inhibitors (lactacystin and N -acetyl-Nor-Leu-Leu-Al) all result in a reduction in the proportion of transected axons able to reform growth cones. (jneurosci.org)
Glycine1
- IMSEAR at SEARO: Effect of cycloheximide on intestinal transport of methionine & glycine. (who.int)
Polymyxin1
- from clinical samples by using buffered charcoal yeast extract (BCYE) medium, with and without antimicrobial drugs, including polymyxin B, cycloheximide, and vancomycin. (cdc.gov)
Assay4
- Cycloheximide and disulfoton are positive in the photoclastogencity assay but do not absorb UV irradiation: another example of pseudophotoclastogenicity? (bvsalud.org)
- To explore this further, four coded compounds ( aminotriazole , propantheline bromide , cycloheximide and disulfoton ) were evaluated for their potential response in a photoclastogenicity assay in cultured Chinese hamster ovary (CHO) cells . (bvsalud.org)
- Follow-up testing showed that neither cycloheximide nor disulfoton was positive in the 3T3-NRU assay, the standard in vitro regulatory test for phototoxicity , a result consistent with their inability to absorb UVR. (bvsalud.org)
- The regulation of MMP2 by p-ATF1-T184 was investigated by a series of experiments including quantitative RT-PCR, western blot, gelatin zymography assay, Chromatin immunoprecipitation (ChIP), luciferase reporter assay and cycloheximide experiment. (biomedcentral.com)
Selective2
Receptor3
- Regulation of TCDD-induced degradation of Ah receptor by cycloheximide. (cdc.gov)
- The changes in Fc receptor affinity and maximum binding of the PMN were reversible in short-term culture, an effect which was prevented by cycloheximide. (elsevier.com)
- cycloheximide prevented enhancement of phagocytosis, Fc receptor capacity, and colony formation. (aai.org)
Cells4
- The results showed that cycloheximide , disulfoton and tetracycline were clastogenic in CHO cells following UVR exposure (solar-simulated light at 700 mJ/cm(2)) but not in the absence of UVR. (bvsalud.org)
- Cycloheximide is a drug that inhibits protein synthesis by cells. (qualityassignments.net)
- https://qualityassignments.net/wp-content/uploads/2020/07/QAlogo3.png 0 0 Paul https://qualityassignments.net/wp-content/uploads/2020/07/QAlogo3.png Paul 2022-03-24 04:58:53 2022-03-24 04:58:53 Cycloheximide is a drug that inhibits protein synthesis by cells. (qualityassignments.net)
- Culture requires growth in cycloheximide-treated McCoy or HeLa cells, and even under these conditions, yields of only 30-50% are reported. (medscape.com)
Gene1
- Furthermore, it was demonstrated that cold shock causes rapid translation repression in A. thaliana seedlings and that the elongation factor LOS1 is involved in cold- or cycloheximide-induced gene expression, thus implicating translational machinery in the regulation of temperature signalling in plants. (bl.uk)
Regulation1
- Moreover, treatment with cycloheximide inhibited the compensatory regulation. (tubitak.gov.tr)
Drugs1
- The scientists built upon previous research, which tested microfluidic screening processes for chemicals, such as potassium cyanide, cycloheximide and chemotherapy drugs. (newswise.com)
Medium1
- cycloheximide 0.5 g/L were added to the Also, Sharquie, Al-Rubyae and Al-Rawi medium. (who.int)
Effect2
- Pretreatment of myocardial preparation with cycloheximide in low concentrations completely blocks the inhibitory insulin effect on Ca2+-current due, probably, to a decrease in peptide formation. (ac.ru)
- Possible mechanisms of modifying effect of cycloheximide on the function of insulin-dependent regulatory system in the myocardium, are discussed. (ac.ru)
Culture1
- Diagnosis is made by microscopic examination followed by fungal culture using cycloheximide-free media. (logicalimages.com)
Suggest1
- These data suggest that both cycloheximide and disulfoton are pseudophotoclastogens, like zinc oxide . (bvsalud.org)
Method1
- Components: Cycloheximide (100 mg/ml) - Method: Prepared in DMSO. (labcloudinc.com)
Resistance to cycloheximide1
- Confers resistance to cycloheximide. (uniprot.org)
Inhibition5
- This suggests that rapidly transported DBH, depleted from the nerve by cycloheximide‐induced inhibition of protein synthesis, is more highly associated with intraneuronal particles than is slowly transported or stationary DBH. (elsevier.com)
- 15. Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme. (nih.gov)
- Conversely, cycloheximide significantly (P=0.0001) inhibited biofilm formation in all C. albicans strains(126) and its effect was even 3 fold more pronounced than EDTA inhibition, probably due to its attenuation of proteins (enzymes) and/or complex molecules necessary for biofilm formation. (bioinformation.net)
- Cycloheximide inhibition decreased NH₄ ⁺ -¹⁵N enrichment and reduced N₂O fluxes while reducing the contribution of codenitrification to total N₂O fluxes by ≥ 66 and ≥ 42%, respectively. (lincoln.ac.nz)
- Model Structure * * ABSTRACT: Inhibition of protein synthesis by cycloheximide blocks * subsequent division of a mammalian cell, but only if the cell * is exposed to the drug before the restriction point (i.e. within * the first several hours after birth). (nih.gov)
Inhibitor of protein synthesis1
- When the HepG2 cells were treated with cycloheximide, a general inhibitor of protein synthesis, the loss of CYP3A4 protein was accelerated by cotreatment with either proteasome or NF-kappaB inhibitors. (nih.gov)
Translation elongation2
- They tested two translation inhibitors -- cycloheximide (inhibitor of translation elongation) and emetine (inhibits 40S ribosomal protein S14) for their ability to inhibit SARS-CoV-2 replication. (scienceboard.net)
- The third Ribo-seq library was prepared from cells treated with the translation elongation inhibitor cycloheximide (CHX), and gives a snap-shot of actively translating ribosomes across the body of the translated ORF. (ucsc.edu)
McCoy3
- A through I. In a study of 194 cervical and urethral specimens from women, cycloheximide treatment of McCoy cells was found to be more efficient than 5-iodo-2-deoxyuridine treatment for the isolation of C. trachomatis. (nih.gov)
- We have compared the two monoclonal antibodies with our routine isolation method, which utilises Giemsa staining of cycloheximide treated McCoy cell cultures. (bmj.com)
- It can be grown on any of these cell lines treated with cycloheximide: McCoy, HEp-2, HeLa, or buffalo green monkey cells. (medscape.com)
Proteins1
- Cycloheximide inhibited the accumulation of ubiquitinylated proteins and hampered A0-induced cell death process. (gla.ac.uk)
Inhibits2
- Administration of cycloheximide inhibits the rise in N-acetyltransferase and N-acetylserotonin, but not melatonin. (nih.gov)
- Cycloheximide, 50ml (1mg/ml) Solution inhibits brewers yeast growth and is used as an ingredient in media plate preparation. (brewculture.com)
Antifungal2
- The aim of this study was to determine in vitro effects of EDTA, cycloheximide, and heparin-benzyl alcohol preservative on C. albicans (126) and non-albicans (31)vaginal yeast isolates biofilm formations and their susceptibility against three antifungal Etest strips. (bioinformation.net)
- To improve the ability of the yeast to remove the metals, it was mutated by Nitrosoguanidine, and among different mutants, it was found that three of them designated as Sc6-1, Sc6-2 and Sc6-3, were more resistant to the antifungal cycloheximide in a concentration of 5 and 10 µg/ ml. (edu.iq)
Chloramphenicol1
- We isolated P. orbiculare on a peptone-glucose-yeast extract medium containing chloramphenicol and cycloheximide, and overlaid with olive oil. (who.int)
Neurons1
- When neurons are treated with cycloheximide to examine for newly synthesized Nrf-2, we found that the Nrf-2 stability is largely decreased. (nih.gov)
Sabouraud1
- Sur 35 échantillons de boue cultivés sur de la gélose de Sabouraud avec du cycloheximide et du chloramphénicol, 326 colonies fongiques appartenant à sept espèces ont été isolées. (who.int)
Stabilization2
- Superinducibility by cycloheximide is due to two effects: prolonged transcription and stabilization of mRNAs. (nih.gov)
- Translation ribosome profiling (TRP) recognizes RNA fragments covered with the ribosome equipment after stabilization with cycloheximide (18). (stopvivisection.info)
Antibiotic1
- The glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. (nih.gov)
Cells6
- Cycloheximide was used at concentrations that depressed, but did not completely inhibit, the metabolism of the eucaryotic host cells. (nih.gov)
- In studies on different immunotypes of C. trachomatis cultured in the yolk sac of embryonated hen eggs, the cycloheximide technique was compared with a method using pretreatment of cells with 5-iodo-2-deoxyuridine. (nih.gov)
- Among such cells, a subpopulation responsive to the bitter taste stimuli quinine and cycloheximide, and aversive concentrations of sodium, cofired to agonists of nocisensitive TRP channels, including capsaicin, mustard oil, and noxious heat. (jneurosci.org)
- Multiple green fluorescent punctae representing the US27 protein were present in WT- and US27synthesis, we treated cells with cycloheximide overnight to block protein synthesis prior to computer virus contamination. (casein-kinases.com)
- If cycloheximide is later removed from the culture medium, treated * cells will return to the division cycle, showing a complex pattern * of division times post-treatment, as first measured by Zetterberg * and colleagues. (nih.gov)
- HeLa cells were infected with (A-B) or serovar E (C-D) and exposed to the DAMPs cAMP (1 mM), ATP (1 mM), or ADO (50 M, plus 25 M EHNA) in incubation medium, in the presence (A, C) or absence (B, D) of 1 1 g/ml cycloheximide, immediately after infection. (bioednet.org)
Bacterial1
- An overview of Genetic Toxicology Bacterial Mutagenicity study conclusions related to Cycloheximide (66-81-9). (nih.gov)
Stimulation1
- Cycloheximide attenuated Ang-(1-7) stimulation of NET expression suggesting that Ang-(1-7) stimulates NET synthesis. (conicet.gov.ar)
Accumulation2
- 4. A single local injection of cycloheximide (10 micrograms/site) did not inhibit neutrophil accumulation or oedema formation produced by 100% Arthus anti-sera. (ox.ac.uk)
- Cycloheximide (10 micrograms/site) did not significantly inhibit the neutrophil accumulation induced by LPS (0.1 microgram/site). (ox.ac.uk)
Gene2
- 2001) Synthesis of several light-harvesting complex I polypeptides is blocked by cycloheximide in symbiotic chloroplasts in the sea slug, Elysia chlorotica (Gould): A case for horizontal gene transfer between alga and animal? (seaslugforum.net)
- This increase in FQR1 mRNA abundance is not diminished by the protein synthesis inhibitor cycloheximide, demonstrating thatFQR1 is a primary auxin-response gene. (ipb-halle.de)
Supplement1
- Resumen en inglés ABSTRACT Influence of long-term palm and corn oil diet supplement administration on rabbit aortic rings vasoconstriction and vasodilatation. (worldwidescience.org)
Infection1
- They were produced at early (3h) and immediate-early (2h) times post-infection respectively as determined by cycloheximide and phosphonoacetic acid treatment but were continuously expressed up to at least 24h post-infection. (bl.uk)
Treatment3
- Unexpectedly, 5-methoxytryptophol increases after cycloheximide treatment. (nih.gov)
- Cycloheximide treatment did not block the ICI effect. (nih.gov)
- Recovery of enzymatic activity in vivo, which occurred within 24 h of low-dose MDMA treatment, appeared not to involve synthesis of new enzyme protein, because the return of enzymatic activity was not prevented by prior cycloheximide. (erowid.org)
Candida1
- El Candida score resultó de 3 en el 77.7% de los casos. (bvsalud.org)
Effects4
- Effects of cycloheximide and aminophylline on 5-methoxytryptophol and melatonin contents in the chick pineal gland. (nih.gov)
- These effects were inhibited by cycloheximide. (gla.ac.uk)
- Resumen en inglés Purpose: To study the effects of mitomycin-C (MMC) on the corneal endothelium after pterygium surgery. (worldwidescience.org)
- 1. In this study, the effects of a protein synthesis inhibitor, cycloheximide, and a soluble tumour necrosis factor (TNF) binding/IgG fusion protein, p55-sf2, on the priming and challenge stages of the local Shwartzman reaction (LSR) were assessed and compared with their effects on the acute inflammatory response induced by recombinant human tumour necrosis factor-alpha (rhTNF), lipopolysaccharide (LPS) and a reversed passive Arthus (RPA) reaction in rabbit skin. (ox.ac.uk)
Injection1
- In the LSR, priming i.d. injections of LPS caused a dose-dependent increase in haemorrhage and plasma leakage at skin sites after challenge with LPS (two injections of 100 micrograms, i.v.). Co-injection of a single dose of cycloheximide (10 micrograms/site) with LPS (30 micrograms/site) caused a marked reduction in the amount of haemorrhage. (ox.ac.uk)
Presence4
- This decrease in HDAC2 protein expression was reversed by curcumin even in the presence of cycloheximide, a protein synthesis inhibitor. (nih.gov)
- All 10 RNAs were superinduced in the presence of cycloheximide. (nih.gov)
- The presence of keratinophilic fungi occur abundantly in the keratinophilic fungi was confirmed by sludge environment and the influence of en lowpower microscopic examination. (who.int)
- presence of the paternal pronucleus assists embryo in overcoming cycloheximide induced abnormalities in zygotic mitosis. (cameras2019.info)
Responses1
- Local cycloheximide (10 micrograms/site) administered immediately before LSR challenge did not affect the responses produced in the LSR. (ox.ac.uk)
Medium2
- Tissue culture medium and serum were from Invitrogen Life Technologies (Carlsbad, CA). Forskolin, cycloheximide, indomethacin, phorbol 12-myristate 13-acetate (PMA), 5-bromo-2'-deoxyuridine (BrdU), protease inhibitor mixture (P-2714), 3-isobutyl-1-methylxanthine (IBMX), and phosphatase inhibitor cocktail I were from Sigma (St. Louis, MO). (medscape.com)
- cycloheximide 0.5 g/L were added to the Also, Sharquie, Al-Rubyae and Al-Rawi medium. (who.int)
Administration1
- Administration of cycloheximide, 10 mg/kg s.c. led within 4 h to an approx 30% reduction of dopamine‐β‐hydroxylase (DBH) activity in the abdominal portion of rat sciatic nerves. (elsevier.com)
Plasma1
- Resumen en inglés Using endothelium-denuded rat aortic rings incubated in Ca2+-free solution, we assessed the ability of testosterone to influence the contractile effect of phenylephrine, and the increase in resting tone (IRT) associated with Ca2+ ability to cross the plasma membrane. (worldwidescience.org)
Method1
- The cycloheximide method gave greater numbers of inclusion-forming units per cover slip for all the immunotypes of trachoma-inclusion conjunctivitis agents tested, i.e. (nih.gov)
Time2
- Over a 3-h time course, nevertheless, alpha-thrombin-induced secretion of PDGFc was not prevented by cycloheximide. (aidstar-one.com)
- parental or Wnt3a+) treated with 25?g/ml cycloheximide (CHX) or dimethyl sulfoxide (DMSO) for the time indicated (0C24?h). b Assessment of complementary DNA (cDNA) sequence in control liver (CTRL) and TKO HCC. (yearofevolution.org)