Cycloheximide
Protein Synthesis Inhibitors
Compounds which inhibit the synthesis of proteins. They are usually ANTI-BACTERIAL AGENTS or toxins. Mechanism of the action of inhibition includes the interruption of peptide-chain elongation, the blocking the A site of ribosomes, the misreading of the genetic code or the prevention of the attachment of oligosaccharide side chains to glycoproteins.
Dactinomycin
A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)
Puromycin
Protein Biosynthesis
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells, Cultured
Emetine
The principal alkaloid of ipecac, from the ground roots of Uragoga (or Cephaelis) ipecacuanha or U. acuminata, of the Rubiaceae. It is used as an amebicide in many different preparations and may cause serious cardiac, hepatic, or renal damage and violent diarrhea and vomiting. Emetine inhibits protein synthesis in EUKARYOTIC CELLS but not PROKARYOTIC CELLS.
Transcription, Genetic
Enzyme Induction
Anisomycin
Pactamycin
Gene Expression Regulation
Depression, Chemical
The decrease in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
Tumor Cells, Cultured
Blotting, Northern
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Chloramphenicol
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Tetradecanoylphorbol Acetate
Fibroblasts
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Dose-Response Relationship, Drug
Colchicine
Amanitins
Cyclic peptides extracted from carpophores of various mushroom species. They are potent inhibitors of RNA polymerases in most eukaryotic species, blocking the production of mRNA and protein synthesis. These peptides are important in the study of transcription. Alpha-amanitin is the main toxin from the species Amanitia phalloides, poisonous if ingested by humans or animals.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
Liver
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Bucladesine
Ornithine Decarboxylase
Peptide Biosynthesis
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Interleukin-1
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Biological Transport
Carbon Isotopes
Tumor Necrosis Factor-alpha
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Cyclic AMP
Deoxyadenosines
Polyribosomes
A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
L Cells (Cell Line)
Stimulation, Chemical
The increase in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
Half-Life
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Rats, Inbred Strains
Enzyme Activation
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Antimetabolites
Aminoisobutyric Acids
Gene Expression
Enzyme Inhibitors
Ribosomes
Culture Techniques
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Bcl-2 and Bcl-XL serve an anti-inflammatory function in endothelial cells through inhibition of NF-kappaB. (1/5972)
To maintain the integrity of the vascular barrier, endothelial cells (EC) are resistant to cell death. The molecular basis of this resistance may be explained by the function of antiapoptotic genes such as bcl family members. Overexpression of Bcl-2 or Bcl-XL protects EC from tumor necrosis factor (TNF)-mediated apoptosis. In addition, Bcl-2 or Bcl-XL inhibits activation of NF-kappaB and thus upregulation of proinflammatory genes. Bcl-2-mediated inhibition of NF-kappaB in EC occurs upstream of IkappaBalpha degradation without affecting p65-mediated transactivation. Overexpression of bcl genes in EC does not affect other transcription factors. Using deletion mutants of Bcl-2, the NF-kappaB inhibitory function of Bcl-2 was mapped to bcl homology domains BH2 and BH4, whereas all BH domains were required for the antiapoptotic function. These data suggest that Bcl-2 and Bcl-XL belong to a cytoprotective response that counteracts proapoptotic and proinflammatory insults and restores the physiological anti-inflammatory phenotype to the EC. By inhibiting NF-kappaB without sensitizing the cells (as with IkappaBalpha) to TNF-mediated apoptosis, Bcl-2 and Bcl-XL are prime candidates for genetic engineering of EC in pathological conditions where EC loss and unfettered activation are undesirable. (+info)NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae. (2/5972)
A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. Molecular genetic analysis of NMD3 revealed that it is an essential gene required for stable 60S ribosomal subunits. Cells bearing a temperature-sensitive allele of NMD3 had decreased levels of 60S subunits at the nonpermissive temperature which resulted in the formation of half-mer polysomes. Pulse-chase analysis of rRNA biogenesis indicated that 25S rRNA was made and processed with kinetics similar to wild-type kinetics. However, the mature RNA was rapidly degraded, with a half-life of 4 min. Nmd3p fractionated as a cytoplasmic protein and sedimented in the position of free 60S subunits in sucrose gradients. These results suggest that Nmd3p is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. Putative orthologs of Nmd3p exist in Drosophila, in nematodes, and in archaebacteria but not in eubacteria. The Nmd3 protein sequence does not contain readily recognizable motifs of known function. However, these proteins all have an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization. (+info)delta-Aminolevulinate synthetases in the liver cytosol fraction and mitochondria of mice treated with allylisopropylacetamide and 3,5-dicarbethoxyl-1,4-dihydrocollidine. (3/5972)
Hepatic delta-aminolevulinate (ALA) synthetase was induced in mice by the administration of allylisopropylacetamide (AIA) and 3,5-dicarbethoxy-1,4-dihydrocollidine (DDC). In both cases, a significant amount of ALA synthetase accumulated in the liver cytosol fraction as well as in the mitochondria. The apparent molecular weight of the cytosol ALA synthetase was estimated to be 320,000 by gel filtration, but when the cytosol ALA synthetase was subjected to sucrose density gradient centrifugation, it showed a molecular weight of 110,000. In the mitochondria, there were two different sizes of ALA synthetase with molecular weights of 150,000 and 110,000, respectively; the larger enzyme was predominant in DDC-treated mice, whereas in AIA-treated mice and normal mice the enzyme existed mostly in the smaller form. When hemin was injected into mice pretreated with DDC, the molecular size of the mitochondrial ALA synthetase changed from 150,000 to 110,000. The half-life of ALA synthetase in the liver cytosol fraction was about 30 min in both the AIA-treated and DDC-treated mice. The half-life of the mitochondrial ALA synthetase in AIA-treated mice and normal mice was about 60 min, but in DDC-treated mice the half-life was as long as 150 min. The data suggest that the cytosol ALA synthetase of mouse liver is a protein complex with properties very similar to those of the cytosol ALA synthetase of rat liver, which has been shown to be composed of the enzyme active protein and two catalytically inactive binding proteins, and that ALA synthetase may be transferred from the liver cytosol fraction to the mitochondria with a size of about 150,000 daltons, followed by its conversion to enzyme with a molecular weight of 110,000 within the mitochondria. The process of intramitochondrial enzyme degradation seems to be affected in DDC-treated animals. (+info)Microvessels from Alzheimer's disease brains kill neurons in vitro. (4/5972)
Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease. (+info)Expression of thrombospondin-1 in ischemia-induced retinal neovascularization. (5/5972)
Thrombospondin-1 is an extracellular matrix protein that inhibits endothelial cell proliferation, migration, and angiogenesis. This study was performed to investigate the role of thrombospondin-1 in ischemic retinal neovascularization. In a murine model of retinal neovascularization, thrombospondin-1 mRNA was increased from postnatal day 13 (P13), with a threefold peak response observed on P15, corresponding to the time of development of retinal neovascularization. Prominent expression of thrombospondin-1 was observed in neovascular cells, specifically, cells adjacent to the area of nonperfusion. It has been suggested that vascular endothelial growth factor (VEGF) plays a major role in ischemia-induced retinal neovascularization of this model, so we studied the effects of VEGF on thrombospondin-1 expression. In bovine retinal microcapillary endothelial cells, VEGF induced a biphasic response of thrombospondin-1 expression; VEGF decreased thrombospondin-1 mRNA 0.41-fold after 4 hours, whereas it increased, with a threefold peak response, after 24 hours. VEGF-induced endothelial cell proliferation was completely inhibited by exogenous thrombospondin-1 and increased by 37.5% with anti-thrombospondin-1 antibody. The present findings suggest that, in the ischemic retina, retinal neovascular cells increase thrombospondin-1 expression, and VEGF may stimulate endogenous thrombospondin-1 induction, which inhibits endothelial cell growth. VEGF-mediated thrombospondin-1 induction in ischemia-induced angiogenesis may be a negative feedback mechanism. (+info)5'-Nucleotidase activity of mouse peritoneal macrophages. II. Cellular distribution and effects of endocytosis. (6/5972)
The diazonium salt of sulfanilic acid (DASA) can inactivate about 80% of the total 5'-nucleotidase of viable macrophages. The remaining 20% can be inactivated if the cells are first lysed in detergent, and presumably represents an intracellular pool of 5'-nucleotidase. The bulk of this pool may represent cytoplasmic vesicles derived from plasma membrane by endocytosis. This internal compartment is expanded up to threefold immediately after the cells have ingested a large latex load. This is consistent with previous observations on the internalization of 5'-nucleotidase in latex phagosomes. In latex-filled cells this intracellular pool of enzyme is inactivated over a few hours, and the cells then slowly increase their enzyme activity to nearly normal levels. However, 24 h after latex ingestion the metabolism of 5'-nucleotidase in these recovered cells is abnormal, as the rate of enzyme degradation is about twice the normal rate, and the DASA-insensitive enzyme pool in these cells is strikingly diminished. This may reflect effects of the accumulated indigestible particles on the fate of incoming pinocytic vesicles or on newly synthesized plasma membrane precursor. Another endocytic stimulus, concanavalin A, also reduces the total cell 5'-nucleotidase activity. This effect, which is time and temperature dependent, can be prevented by the competitive sugar alpha-methyl mannose. The concanavalin A inhibition can be reversed in the absence of new protein synthesis or in cells cultivated in serum-free conditions. It is not known whether the effect of concanavalin A on 5'-nucleotidase depends upon the interiorizaiton of plasma membrane or is strictly associated with events at the cell surface. (+info)CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo. (7/5972)
cAMP activated insertion of the cystic fibrosis transmembrane conductance regulator (CFTR) channels from endosomes to the apical plasma membrane has been hypothesized to regulate surface expression and CFTR function although the physiologic relevance of this remains unclear. We previously identified a subpopulation of small intestinal villus epithelial cells or CFTR high expressor (CHE) cells possessing very high levels of apical membrane CFTR in association with a prominent subapical vesicular pool of CFTR. We have examined the subcellular redistribution of CFTR in duodenal CHE cells in vivo in response to the cAMP activated secretagogue vasoactive intestinal peptide (VIP). Using anti-CFTR antibodies against the C terminus of rodent CFTR and indirect immunofluorescence, we show by quantitative confocal microscopy that CFTR rapidly redistributes from the cytoplasm to the apical surface upon cAMP stimulation by VIP and returns to the cytoplasm upon removal of VIP stimulation of intracellular cAMP levels. Using ultrastructural and confocal immunofluorescence examination in the presence or absence of cycloheximide, we also show that redistribution was not dependent on new protein synthesis, changes in endocytosis, or rearrangement of the apical cytoskeleton. These observations suggest that physiologic cAMP activated apical membrane insertion and recycling of CFTR channels in normal CFTR expressing epithelia contributes to the in vivo regulation of CFTR mediated anion transport. (+info)Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (8/5972)
A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided. (+info)
The effects of anisomycin (a protein synthesis inhibitor) on spatial
learning and memory in CA1 region of rats hippocampus
HTLV receptor is an early T-cell activation marker whose expression requires de novo protein synthesis | Blood | American...
Prevalent deficiency in tumor cells of cycloheximide-induced cycle arr by E E. Medrano and A B. Pardee
Export Data and Price of Cycloheximide | www.eximpulse.com
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McClean: Cycloheximide Stock Solution - OpenWetWare
Protective effect of cycloheximide upon protein synthesis by l5178y ce by J E. Fuhr, M Overton et al.
cycloheximide | VWR
Gene expression
Effect of protein synthesis inhibitors on the hormonal induction of alanine and aspartate aminotransferases in the liver of...
Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities | Journal of Cell Science
Plouhinec JL et al. (2014),
Pax3 and Zic1 trigger the early neural crest ge... -
Xenbase Paper
Hypoxic neuronal necrosis: Protein synthesis-independent activation of a cell death program | Proceedings of the National...
Search Results for oxytocin | joe
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Search Results | Journal of Cell Biology | Rockefeller University Press
Cycloheximide - Creative Peptides
A) Western blot demonstrating cycloheximide effects of | Open-i
Protein Synthesis Inhibitors | Flashcards
TNFα-induced lysosomal membrane permeability is downstream of MOMP and triggered by caspase-mediated NDUFS1 cleavage and ROS...
SynPharm: A New Annexe to the Guide to PHARMACOLOGY | guidetopharmacology blog
Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation...
Put Your Title Here
Cycloheximide - Wikipedia
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The Effect of Cycloheximide on the Replication of Measles Virus | Microbiology Society
Constitutive Nuclear Factor κB Activity Is Required for Survival of Activated B Cell-like Diffuse Large B Cell Lymphoma Cells ...
Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response...
SR9243 | CAS#1613028-81-1 | LXR inverse agonist | MedKoo
Cycloheximide, Crystalline | Krackeler Scientific, Inc.
Anisomycin | Abcam
Anisomycin | Purity |98% | CAS 22862-76-6 | Alomone Labs
N-methyl-D-aspartate receptor-mediated neuroprotection in cerebellar granule cells requires new RNA and protein synthesis | PNAS
Download Free Protein Synthesis Like A Banana Split Answer Key, High quality vector files
Protein Synthesis Worksheet. Worksheets. Doriandnimo Thousands of Printable Activities
Novel cycloheximide derivatives with antimicrobial activity against Legionella pneumophila
S propranolol synthesis - How to take dapoxetine 60
Impact of Micro and Macroporous TFF Membranes on Product Sieving and Chromatography Loading for Perfusion Cell Culture | Aspen...
Protein Synthesis | Your Article Library
A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina | Development
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chloramphenicol (thing) by witnie - Everything2.com
Transcriptional regulation of prolactin receptor gene expression by sodium butyrate in MCF-7 human breast cancer cells | Garvan...
Biochemical characterization of spiromesifen and spirotetramat as lipid synthesis inhibitors on cotton leaf worm, Spodoptera...
Apoptosis induced by mild hyperthermia in human and murine tumour cell lines: A study using electron microscopy and DNA gel...
北京大学医学部机构知识库([email protected]): Cycloheximide and actinomycin D delay death and affect bcl-2, bax, and ice gene expression in...
kbrin.a-bldg.louisville.edu
A Physical Model Illustrating Protein Synthesis on the Ribosome | The American Biology Teacher | University of California Press
Thymidine (DThyd) | DNA/RNA Synthesis Inhibitor | MedChemExpress
Despite intensive analysis zero therapies targeted contrary to the oncogenic EGFRvIII | Imaging Proteolysis by Living Human...
Protein synthesis: steps and process
science protein synthesis Flashcards - Cram.com
Turning over the keys to rural businesses : Community Features : UMN Extension
What are the steps involved in protein synthesis when you start with the DNA code in the nucleus and end up with a polypeptide...
What Does the Term protein Synthesis Mean? | Reference.com
CiNii Articles -
Mycotrienin II, a translation inhibitor that prevents ICAM-1 expression induced by pro-inflammatory...
CHX10抗体- ChIP Grade|Abcam中国|Anti-CHX10抗体- ChIP Grade(ab16141)
Glutarimide
It is the core of a variety of drugs, including barbituric acid and cycloheximide, a potent inhibitor of protein synthesis. ... Hugh D. Sisler, Malcolm R. Siegel; Cycloheximide and Other Glutarimide Antibiotics; Mechanism of Action pp 283-307 v t e. ...
Chlamydosauromyces
It is also cycloheximide resistant. C. punctatus can reproduce both in sexual and asexual forms. The teleomorph phase is ...
Lomentospora prolificans
This species is sensitive to cycloheximide. As this species may be slow to emerge from clinical materials, specimens in which ...
Acetoxycycloheximide
Cycloheximide Mahy, Brian W J (2001). A dictionary of virology (3. ed.). San Diego, Calif. [u.a.]: Academic Press. pp. 2. ISBN ... It can be considered as the acetylated analogue of cycloheximide. It is a potent protein synthesis inhibitor in animal cells ...
Cryptococcus adeliensis
... also grows on 0.01% cycloheximide. Cryptococcus adeliensis sp. nov., a xylanase producing ...
Streptomyces albulus
... produces acetoxycycloheximide, aciphenol, albanoursin and cycloheximide. List of Streptomyces species LPSN ...
Cyanidioschyzon merolae
Yagisawa F; Nishida K; Okano Y; Minoda A; Tanaka K; Kuroiwa T (2004). "Isolation of cycloheximide-resistant mutants of ...
Geomyces pannorum
... is resistant to the antifungal agent cycloheximide. However the growth of this species is inhibited by ...
Eukaryotic ribosome
Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin". Nat Chem Biol. 6 (3): 209-217. doi: ... One toxic inhibitor of eukaryotic translation elongation is the glutarimide antibiotic cycloheximide (CHX), which has been co- ...
Lactimidomycin
March 2010). "Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin". Nature Chemical Biology. 6 ...
Hammonton, New Jersey
Alma Joslyn Whiffen-Barksdale (1916-1981), mycologist who discovered cycloheximide. Gary Wolfe (born 1967), professional ...
Bacterivore
Adding cycloheximide can inhibit the growth of bacterivores without affecting some bacterial species but it was shown to ... Tremaine, Sarah C. Mills, Aaron L. (1987). "Inadequacy of the Eucaryote Inhibitor Cycloheximide in Studies of Protozoan Grazing ... "Cycloheximide: Aspects of Inhibition of Protein Synthesis in Mammalian Cells". Science. 146 (3650): 1474-1476. Bibcode:1964Sci ...
Alma Joslyn Whiffen-Barksdale
... (October 25, 1916 - July 5, 1981) was a U.S. mycologist who discovered cycloheximide. She was ... List of mycologists Cycloheximide Achlya bisexualis "Alma Whiffen Barksdale (1916-1981)". Smithsonian Institution Archives. ... While there, she discovered the chemical cycloheximide (trade name Actidione), an anti-fungal and anti-bacterial agent produced ...
Mucor racemosus
It has been shown to adapt to famous antibiotics like cycloheximide, trichodermin and amphotericin B. Cells adapted to ... Shearer G, Jr; Sypherd, PS (March 1988). "Cycloheximide efflux in antibiotic-adapted cells of the fungus Mucor racemosus". ... cycloheximide particularly have been observed to be 40-times more resistant than non-adapted cells to the drug. These adapted ...
Ribosome profiling
This is commonly performed with cycloheximide but other chemicals can be employed. It is also possible to forgo translation ... RNA-ribosome complexes Cycloheximide Nucleases Phenol/Chloroform Reverse transcriptase dNTPs Sequencing method-cDNA library. ... The other elongating regions can be detected by adding antibiotics like cycloheximide that inhibit translocation, ...
Harold M. Weintraub
"Conservative segregation of parental histones during replication in the presence of cycloheximide". Proc Natl Acad Sci USA. 76 ...
C-5 sterol desaturase
Abe Fumiyoshi; Hiraki Toshiki (2009). "Mechanistic role of ergosterol in membrane rigidity and cycloheximide resistance in ...
Microsporum nanum
Like many other dermatophytes, M. nanum is tolerant of the antifungal agent cycloheximide. In addition, M. nanum also exhibits ...
Parthenogenesis
Treatment with cycloheximide, a non-specific protein synthesis inhibitor, enhances parthenote development in swine presumably ... "Effects of Cycloheximide on Parthenogenetic Development of Pig Oocytes Activated by Ultrasound Treatment". Journal of ...
Adrenal ferredoxin
1988). "Human adrenodoxin: cloning of three cDNAs and cycloheximide enhancement in JEG-3 cells". J. Biol. Chem. 263 (7): 3240-4 ...
Cln3
Finally, it was also shown that this delay occurred even with short pulses of cycloheximide, confirming that an unstable ... It was later shown that treatment with the protein synthesis inhibitor cycloheximide delayed Start in yeast, indicating that ... "Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide". Experimental Cell Research ...
Cyclin D1
Baliga BS, Pronczuk AW, Munro HN (August 1969). "Mechanism of cycloheximide inhibition of protein synthesis in a cell-free ... Obrig TG, Culp WJ, McKeehan WL, Hardesty B (January 1971). "The mechanism by which cycloheximide and related glutarimide ...
Trichophyton rubrum
... rubrum cultures can be isolated on both cycloheximide-containing media and cycloheximide-free media. The latter are ... In primary outgrowth on Sabouraud dextrose agar with cycloheximide and antibacterials, contaminating organisms may cause ...
Trichophyton tonsurans
CLS growth is essentially unaffected by antibiotic treatment with chloramphenicol, as well as by cycloheximide. After 5 days of ...
Cunninghamella bertholletiae
Unlike in the case of dimorphic pathogenic fungi, growth of C. bertholletiae is inhibited by cycloheximide. As a member of the ...
Trichosporon asteroides
T. asteroides grows in a range of media including in the presence of the antifungal agent, cycloheximide. Colonies of T. ...
Neothramycin
MC916-C4, a cycloheximide-producing strain, by Umezawa et al. Subsequent testing has shown its capabilities as an anticancer ...
Anisomycin
Cycloheximide Anisomycin Archived January 19, 2012, at the Wayback Machine from Sigma Aldrich Grollman, Arthur P. (1967). " ...
Cryptococcus consortionis
This species required thiamine for proper growth, and its growth is slowed by small amounts of cycloheximide. C. consortionis ...
Exophiala dermatitidis
The fungus has been selectively isolated using high temperature incubation (at 40 °C) on media containing cycloheximide. ...
Cycloheximide - Wikipedia
"Cycloheximide - The Antimicrobial Index Knowledgebase - TOKU-E". antibiotics.toku-e.com.. *^ "Cycloheximide-Naramycin A; ... Cycloheximide is a eukaryote protein synthesis inhibitor, produced by the bacterium Streptomyces griseus. Cycloheximide exerts ... it responds to cycloheximide, so, it should be cultured in a medium free of cycloheximide. ... Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. ...
cycloheximide (CHEBI:27641)
... is a piperidones (CHEBI:48589) cycloheximide (CHEBI:27641) is a secondary alcohol (CHEBI:35681) ... cycloheximide (CHEBI:27641) is a cyclic ketone (CHEBI:3992) cycloheximide (CHEBI:27641) is a dicarboximide (CHEBI:35356) ... cycloheximide (CHEBI:27641) has functional parent piperidine-2,6-dione (CHEBI:5435) cycloheximide (CHEBI:27641) has role ... cycloheximide (CHEBI:27641) has role protein synthesis inhibitor (CHEBI:48001) cycloheximide (CHEBI:27641) is a antibiotic ...
Cycloheximide - definition of cycloheximide by The Free Dictionary
cycloheximide synonyms, cycloheximide pronunciation, cycloheximide translation, English dictionary definition of cycloheximide ... Cycloheximide - definition of cycloheximide by The Free Dictionary https://www.thefreedictionary.com/cycloheximide ... cycloheximide. Also found in: Medical, Acronyms, Encyclopedia, Wikipedia. cy·clo·hex·i·mide. (sī′klō-hĕk′sə-mīd′, -mĭd). n.. A ... cycloheximide. (ˌsaɪkləʊˈhɛksəˌmaɪd) n. a toxic antibiotic that inhibits protein synthesis, used as a fungicide in agriculture ...
Cycloheximide from microbial, ≥94% (TLC) | 66-81-9
Cycloheximide from microbial, ≥94% (TLC); CAS Number: 66-81-9; EC Number: 200-636-0; Synonyms: Actidione,3-[2-(3,5-Dimethyl-2- ... In yeast strains, cycloheximide has been used as a protein synthesis inhibitor in the cycloheximide chase experiment. ... Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in ...
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McClean: Cycloheximide Stock Solution - OpenWetWare
Weigh out the appropriate amount of cycloheximide and add it to the appropriate amount of DMSO to get a 50mg/ml solution. Use ... Cycloheximide (MP Biomedicals, Cat #100183). *DMSO (Fluka 41639, Ultra for molecular biology; stored in the Flammables cabinet) ... Cycloheximide is bad for you (see below) so be careful with it! ... Megan N McClean: Cycloheximide is BAD for you. Check out the ... Each person in the lab makes up their own stock of cycloheximide and keeps it in their own -20°C box. ...
A Cycloheximide-Sensitive Step in Transverse Microtubule Array Patterning | Plant Physiology
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Reduction of Rat Liver Microsomal Ribonuclease by Cycloheximide | Molecular Pharmacology
Reduction of Rat Liver Microsomal Ribonuclease by Cycloheximide Message Subject (Your Name) has forwarded a page to you from ... The results indicate that the short onset of microsomal RNase reduction produced by cycloheximide may be due to a more rapid ... Reduction of Rat Liver Microsomal Ribonuclease by Cycloheximide. ROBERT T. LOUIS-FERDINAND ... Reduction of Rat Liver Microsomal Ribonuclease by Cycloheximide. ROBERT T. LOUIS-FERDINAND ...
Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after...
Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after ... No significant reduction in the probability of the conditioned response in cycloheximide-treated bees was found when compared ... Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after ... Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after ...
A propos d'un effet paradoxal de la cycloheximide sur l'œuf d'Artemia salina | Development
Cycloheximide, an inhibitor of protein synthesis at the translation level, has a very strong antimitotic activity upon the ... A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina ... A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina ... A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina ...
SID.ir | GENETIC IDENTIFICATION OF CYCLOHEXIMIDE-RESISTANT YEASTS ISOLATED FROM TRADITIONAL DAIRY PRODUCTS
Download Free Full-Text of an article GENETIC IDENTIFICATION OF CYCLOHEXIMIDE-RESISTANT YEASTS ISOLATED FROM TRADITIONAL DAIRY ... The common method to detect LAB is to culture the products on media containing cycloheximide (CHX) to prevent yeast growth by ... Keyword(s): TRADITIONAL DAIRY PRODUCTS, CYCLOHEXIMIDE-RESISTANT YEASTS, GENETIC IDENTIFICATION, KLUYVEROMYCES MARXIANUS. ...
Effect of Cycloheximide on RNA Metabolism Early in Productive Infection with Adenovirus 2 | Journal of Virology
... and cycloheximide-treated cultures demonstrated that the cultures treated with cycloheximide did not have an increased content ... Effect of Cycloheximide on RNA Metabolism Early in Productive Infection with Adenovirus 2. Elizabeth A. Craig, Heschel J. ... The presence of cycloheximide during the early phase of adenovirus 2 replication causes an increase in the virus-specific ... For both classes, RNA from cultures treated with cycloheximide hybridized 5- to 10-fold more than RNA from control-infected ...
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Cycloheximide prevents the de novo polypeptide synthesis required to recover from acetylene inhibition in Nitrosopumilus...
Effects of cycloheximide on gene expression changes in | Open-i
Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were allowed to attach ... pone.0129578.g008: Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were ... pone.0129578.g008: Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were ... was blocked by cycloheximide (Fig 7). The expressions of Wnt target genes, such as LEF1 and Cyc-D1, and several growth factors ...
Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation...
hsp 70 mRNA levels decreased within 1 h after removal of cycloheximide but then appeared to stabilize during the next 2 h (3 h ... Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation ... Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation ... Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation ...
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Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities | Journal of Cell Science
We report that cycloheximide-treated mouse eggs manifest similar temporal changes in the decrease in both cdc2/cyclin B kinase ... Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the ... However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase ... Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities ...
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... sterile-filtered solution of Cycloheximide (Cat. No. 239763) in DMSO. An antifungal antibiotic that inhibits protein synthesis ... InSolution™ Cycloheximide, CAS 66-81-9, is a 100 mg/ml, sterile-filtered solution of Cycloheximide (Cat. No. 239763) in DMSO. ... InSolution™ Cycloheximide, CAS 66-81-9, is a 100 mg/ml, sterile-filtered solution of Cycloheximide (Cat. No. 239763) in DMSO. ... More,, InSolution™ Cycloheximide, CAS 66-81-9, is a 100 mg/ml, sterile-filtered solution of Cycloheximide (Cat. No. 239763) in ...
Effects of cycloheximide and aminophylline on 5-methoxytryptophol and melatonin contents in the chick pineal gland - Surrey...
A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain | Nature Communications
cycloheximide | VWR
Learn more about cycloheximide. We enable science by offering product choice, services, process excellence and our people make ... Description: Cycloheximide is a glutarimide antibiotic derived from a microbial source. Cycloheximide is an antibiotic which is ... Description: CYCLOHEXIMIDE, also known as hizarocin, is used in biomedical research. It inhibits protein synthesis in ... Description: Cycloheximide, Purity: 90%, CAS Number 66-81-9, Formula: C15H23NO4, Synonyms: 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)- ...
Cycloheximide | Austratec
Cycloheximide - Creative Peptides
Cycloheximide acts by inhibiting elongation during protein synthesis. ... Cycloheximide is an antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. ... Cycloheximide is an antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. Cycloheximide ... Cycloheximide is a colorless crystals. Used as a fungicide and as a anticancer drug. ...
mTORC1 couples cyst(e)ine availability with GPX4 protein synthesis and ferroptosis regulation | Nature Communications
Cycloheximide chase analysis. To determine GPX4 protein stability, UMRC6 cells were cultured in media with or without cystine ... In addition, cycloheximide chase analysis showed that cystine starvation did not decrease GPX4 protein half-life (Supplementary ... Sucrose solutions were prepared in polysome buffer (10 mM HEPES, pH 7.4, 100 mM KCl, 5 mM MgCl2 and 100 mg/ml cycloheximide). A ... for 24 h. Then, cycloheximide (50 μg/ml) were added to media and cells were collected at different time points on ice. Protein ...
Presence of cycloheximideApoptosisProtein synthesis inhibitor cycFingerprintSynthesisInhibitionGriseusAddition of cycloheximideProperties of cycloheximideStrainsYeastMRNAFungalDerivativesWidelyAntimicrobialChloramphenicolEffectCellsInfectionIsolateAgarDMSOCultures treatedAntifungal propertiesDegradationIschemiaConcentrationBiomedical researchTreatmentRatsChaseResistantProteinsApoptotic
Presence of cycloheximide7
- The presence of cycloheximide during the early phase of adenovirus 2 replication causes an increase in the virus-specific content of newly synthesized mRNA. (asm.org)
- Oligo(dT)-cellulose chromatography was used to purify the portion of cytoplasmic RNA containing poly(A). The poly(A)-containing RNA from cultures labeled in the presence of cycloheximide hybridized to viral DNA 32% as compared to 2.2% for RNA from control cultures. (asm.org)
- Therefore, the increased hybridization of cytoplasmic RNA synthesized in the presence of cycloheximide is caused either by reduced transcription of the cellular genome or by greatly increased instability of cellular heterogeneous nuclear RNA. (asm.org)
- Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were allowed to attach overnight and then treated (60 min) with cycloheximide (10 μg/ml) and thereafter switched to medium with or without oleuroepin (20 μM) in the presence of cycloheximide (10 μg/ml) for 24 h. mRNA expression levels of LEF1, Cyc-D1, IGF-1, HGF, VEGF, and KGF were measured by RT-PCR. (nih.gov)
- B) Northern analysis demonstrating that MSX2 induction by Wnt-3A is abolished in the presence of cycloheximide. (nih.gov)
- As expected, BMP-4 mediated induction of MSX2 is not affected by cycloheximide which contrasts with the inhibition of Wnt-3A mediated induction of MSX2 in the presence of cycloheximide. (nih.gov)
- The exposure of HeLa cells to interleukin-1 alpha (IL-1α) in the presence of cycloheximide (CHX) leads to the release of active tumor necrosis factor alpha (TNF-α), eliciting cytocidal effect on these cells. (edu.pl)
Apoptosis3
- Cycloheximide is widely used for selection of cycloheximide-resistant strains of yeast and fungi, for inhibition of eukaryotic contaminants in bacterial microbiology, for controlled inhibition of protein synthesis in cell culture and for detection of short-lived proteins, and for apoptosis induction or facilitation of apoptosis induction by death receptors. (carlroth.com)
- In the present studies, we have evaluated internucleosomal DNA fragmentation and apoptosis within the pancreas and the effects of inhibiting protein synthesis by cycloheximide (CHX) on these phenomena as well as on the severity of pancreatitis. (elsevier.com)
- In summary, our data suggest that apoptosis plays an important role in the development of delayed infarction , and inhibition of apoptosis with cycloheximide significantly reduces the ensuing cerebral infarction in a newborn rat pup model of cerebral hypoxia-ischemia . (bvsalud.org)
Protein synthesis inhibitor cyc3
- The protein synthesis inhibitor cycloheximide was injected via the median ocellus directly into the brain. (jneurosci.org)
- The protein synthesis inhibitor cycloheximide blocks induction by Wnt, consistent with a requirement for newly synthesized beta-catenin protein prior to target gene activation. (nih.gov)
- The protein synthesis inhibitor, cycloheximide was used to study the development of the events leading to pupal ecdysis in Manduca. (elsevier.com)
Fingerprint1
- Fingerprint Dive into the research topics of 'Anti-apoptotic actions of cycloheximide: Blockade of programmed cell death or induction of programmed cell life? (elsevier.com)
Synthesis20
- Cycloheximide is a eukaryote protein synthesis inhibitor , produced by the bacterium Streptomyces griseus . (wikipedia.org)
- Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome ), thus blocking translational elongation . (wikipedia.org)
- Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro ( i.e. outside of organisms). (wikipedia.org)
- In yeast strains, cycloheximide has been used as a protein synthesis inhibitor in the cycloheximide chase experiment. (sigmaaldrich.com)
- Cycloheximide, an inhibitor of protein synthesis at the 'translation' level, has a very strong antimitotic activity upon the egg of Artemia salina , during segmentation. (biologists.org)
- The synthesis of this factor could be extremely sensitive to cycloheximide action. (biologists.org)
- The common method to detect LAB is to culture the products on media containing cycloheximide (CHX) to prevent yeast growth by interfering with protein synthesis. (sid.ir)
- However, cycloheximide (CHX), a widely used eukaryotic protein synthesis inhibitor, but not bacteria-specific protein synthesis inhibitors (kanamycin and gentamycin), inhibited the recovery of NH3-oxidizing activity in N. maritimus. (ovid.com)
- Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation in rat fibroblasts. (asm.org)
- Treatment of rat embryonic fibroblasts with cycloheximide for 6 h after a 20-min interval at 45 degrees C inhibits protein synthesis, including heat shock protein (hsp) synthesis, and results in an accumulation of hsp 70 mRNA, but has no effect on subsequent survival responses to 45 degrees C hyperthermia. (asm.org)
- No increased hsp synthesis was observed in cultures treated with cycloheximide, even though hsp 70 mRNA levels appeared elevated. (asm.org)
- Cycloheximide acts by inhibiting elongation during protein synthesis. (creative-peptides.com)
- Treatment of measles virus-infected cells with cycloheximide results in a three-fold increase of 3 H-uridine incorporation into the 12 to 36S mRNA species and in the inhibition of genomic 50S RNA synthesis. (microbiologyresearch.org)
- These data indicate that the cAMP-induced synthesis of the steroidogenic machinery is not wholly dependent on cycloheximide-sensitive protein mediators. (elsevier.com)
- Correlated changes in energy and protein metabolism were investigated by determining the rates of oxygen consumption at various levels of P CO 2 , of intra- and extracellular pH, and after inhibition of protein synthesis by cycloheximide. (biologists.org)
- Low concentrations of cycloheximide completely blocked the synthesis of B protein but did not inhibit the synthesis of viral structural proteins. (elsevier.com)
- Protective effect of cycloheximide upon protein synthesis by l5178y ce" by J E. Fuhr, M Overton et al. (jax.org)
- Protective effect of cycloheximide upon protein synthesis by l5178y cells exposed to hyperthermia. (jax.org)
- however, the inhibition of PA synthesis by cycloheximide does not prevent the morphological changes or the increased tight junctional permeability, suggesting that PA is not involved in these early TPA-induced events. (nih.gov)
- It is the core of a variety of drugs, including barbituric acid and cycloheximide, a potent inhibitor of protein synthesis. (wikipedia.org)
Inhibition1
- Hybridization-inhibition experiments between RNAs from control- and cycloheximide-treated cultures demonstrated that the cultures treated with cycloheximide did not have an increased content of viral RNA or a new class of viral RNA sequences. (asm.org)
Griseus3
- Cycloheximide is a naturally occurring fungicide produced by the bacterium Streptomyces griseus. (wikipedia.org)
- Cycloheximide (CHX) is an antibiotic produced by S. griseus . (sigmaaldrich.com)
- Cycloheximide is an antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. (creative-peptides.com)
Addition of cycloheximide3
- Translation is halted via the addition of cycloheximide, and the DNA/RNA in the cell is then nuclease treated. (wikipedia.org)
- Addition of cycloheximide or puromycin to such cultures substantially reduced basal levels and markedly attenuated the cAMP-induced accumulation of cytochrome P450scc mRNA, but augmented the accumulation of adrenodoxin and fos mRNAs in additive and multiplicative fashions, respectively. (elsevier.com)
- This study aimed to evaluate the efficacy and safety of the addition of cycloheximide in Optisol-GS media in decreasing the growth of the main Candida spp. (yeast-infection-help.com)
Properties of cycloheximide2
- The translational elongation freezing properties of cycloheximide are also used for ribosome profiling / translational profiling. (wikipedia.org)
- recently published an article describing antimicrobial properties of cycloheximide derivatives with adamantyl moieties against Legionella pneumophila . (toku-e.com)
Strains1
- 9 , 10 Cycloheximide is efficient for mutagenic strains resistant to multiple drugs, 11 and also has the advantage of having a low cost. (yeast-infection-help.com)
Yeast3
- Cycloheximide is a highly effective antibiotic with activity against mold, yeast, and phytopathogenic fungi, with lower activity against bacteria. (wikipedia.org)
- Afterward, 1 mL of the suspension was added to a liquid medium with yeast extract (1%), peptone (4%), glucose (6%), calcium carbonate (1%), 100 mg/L of cycloheximide and 150 mg/L of penicillin at a pH 6.6. (thefreedictionary.com)
- Although most eukaryotic cells are sensitive to the 80S ribosome inhibitor cycloheximide (CYH), naturally occurring CYH resistance is widespread amongst yeast species. (diva-portal.org)
MRNA4
- An inhibitor of mRNA translation, cycloheximide, reduced RNA degradation by hepatic microsomal ribonuclease in a dose-related manner. (aspetjournals.org)
- hsp 70 mRNA levels decreased within 1 h after removal of cycloheximide but then appeared to stabilize during the next 2 h (3 h after drug removal and 9 h after heat shock). (asm.org)
- hsp 70 mRNA accumulation could be further increased by a second heat shock at 45 degrees C for 20 min 6 h after the first hyperthermic exposure in cycloheximide-treated cells. (asm.org)
- Cycloheximide (CH) was added to infected cultures to accumulate early viral mRNA relative to host cell mRNA. (ias.ac.in)
Fungal1
- Nutrient agar with cycloheximide (0.5 mg/ml to suppress fungal growth) [8] and Martin's Rose Bengal agar medium strips were used for the isolation of bacteria and fungi, respectively. (thefreedictionary.com)
Derivatives1
- Ten different cycloheximide derivatives were synthesized in this study, and five were found to inhibit L. pneumophila growth at a concentration of 30 uM or 40 uM. (toku-e.com)
Widely1
- In addition to presenting a broad spectrum of antimicrobial action, cycloheximide is widely used as a preservative agent in culture media to inhibit fungi and bacteria. (yeast-infection-help.com)
Antimicrobial1
- Applied Microbiology, 1970, Vol 20 No. 3, p513-514 "Cycloheximide - The Antimicrobial Index Knowledgebase - TOKU-E". antibiotics.toku-e.com. (wikipedia.org)
Chloramphenicol3
- seaweed) penicillin + streptomycin Mytilus edulis (mollusk) penicillin + streptomycin + chloramphenicol Tetraselmis maculata (phytoplankton) + Tween-80 cycloheximide Crassostrea virginica (mollusk) penicillin + streptomycin Euterpina acutifrons, Temora turbinata, Oncaea sp. (thefreedictionary.com)
- Plasmodia of Physarura polycephalum were exposed to 137cs gamma radiation or certain chemical compounds (chloramphenicol, cycloheximide, and ethidium bromide) and the mitochondria and other organelles of the plasmodia were observed for any ultrastructural changes. (niu.edu)
- When exposure to radiation was combined with either ethidium bromide, chloramphenicol, or cycloheximide no additional ultra- structural changes were observed. (niu.edu)
Effect5
- Mann, NS 1980, ' Enterokinase induced changes in pancreatic protein content: Effect of intraductal azacytidine and cycloheximide ', Gastroenterology , vol. 78, no. 5 II, pp. 1216. (elsevier.com)
- Duda, E 1975, ' Effect of cycloheximide on viral precursor protein B in Sindbis virus infected BHK cells ', Medical biology , vol. 53, no. 5, pp. 368-371. (elsevier.com)
- Effect of cycloheximide on cell division & macromolecular systhesis in root-tip cells of Vicia faba L. (bvsalud.org)
- This study was done to determine the neuroprotective effect of cycloheximide on neonatal hypoxic-ischemic brain injury . (bvsalud.org)
- Morton, DB & Truman, JW 1995, ' Effect of cycloheximide on eclosion hormone sensitivity and the developmental appearance of the eclosion hormone and cGMP regulated phosphoproteins in the CNS of the tobacco hornworm, manduca sexta ', Journal of Receptors and Signal Transduction , vol. 15, no. 5, pp. 773-786. (elsevier.com)
Cells7
- Treating cells with cycloheximide in a time-course experiment followed by Western blotting of the cell lysates for the protein of interest can show differences in protein half-life. (wikipedia.org)
- NCCIT cells were stimulated for 4 hours with CCM (C) or Wnt-3A CM (Wnt) in the presence (20 microgram/ml) or absence (0) of cycloheximide. (nih.gov)
- Cycloheximide and MG115 Treatment of Cells. (aacrjournals.org)
- Cells were seeded at 2 × 10 6 cells/ml in their respective culture medium containing 75 μg/ml of cycloheximide or 10 μ m MG115. (aacrjournals.org)
- Cells were harvested at different time points after cycloheximide treatment and pelleted by centrifugation. (aacrjournals.org)
- Nivalenol, T-2 toxin, and verrucarin A cause rapid and almost quantitative breakdown of polyribosomes in H-HeLa cells, a process which is inhibited by anisomycin, cycloheximide, or trichodermin. (mendeley.com)
- Prevalent deficiency in tumor cells of cycloheximide-induced cycle arrest. (jax.org)
Infection1
- The following represents susceptibility data for a few commonly targeted fungi: Candida albicans: 12.5 μg/ml Mycosphaerella graminicola: 47.2 μg/ml - 85.4 μg/ml Saccharomyces cerevisiae: 0.05 μg/ml - 1.6 μg/ml Neoscytalidium dimidiatum is an Athlete's foot like infection resistant to most antifungals but is rather sensitive to cycloheximide, so, it should be cultured in a medium free of cycloheximide. (wikipedia.org)
Isolate1
- Cycloheximide has been used to isolate dermatophytes and inhibit the growth of fungi in brewing test media. (wikipedia.org)
Agar1
- The purpose of this article is to provide an insight into the share of dermatophytes, yeasts and non-der-matophyte molds as potential pathogens in OM, grown on Sabouraud agar without cycloheximide , as well as the prevalence of positive and negative results of DME, and grown isolates, according to patient sex and age. (thefreedictionary.com)
DMSO1
- Weigh out the appropriate amount of cycloheximide and add it to the appropriate amount of DMSO to get a 50mg/ml solution. (openwetware.org)
Cultures treated1
- For both classes, RNA from cultures treated with cycloheximide hybridized 5- to 10-fold more than RNA from control-infected cultures. (asm.org)
Antifungal properties2
- Cycloheximide has also been used for its antifungal properties to make isolation of bacteria from mixed samples easier. (wikipedia.org)
- Cycloheximide is an antibiotic with significant antifungal properties. (yeast-infection-help.com)
Degradation1
- The decrease in cdc2/cyclin B kinase activity, however, does not seem to be required for the decrease in MAP kinase activity, since the decrease in MAP kinase activity still occurs in cycloheximide-treated eggs that are also incubated in the presence of nocodazole, which inhibits cyclin B degradation and hence the decrease in cdc2/cyclin B kinase. (biologists.org)
Ischemia2
Concentration1
- This is diluted to 100μg/mL to inhibit translation (though you should check this concentration does what you think it does whenever you develop a new protocol using cycloheximide). (openwetware.org)
Biomedical research1
- CYCLOHEXIMIDE , also known as hizarocin, is used in biomedical research. (vwr.com)
Treatment4
- Cycloheximide treatment provides the ability to observe the half-life of a protein without confounding contributions from transcription or translation. (wikipedia.org)
- Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the fertilization-induced Ca2+ transient. (biologists.org)
- However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase activities that are intimately associated with resumption of meiosis. (biologists.org)
- Cycloheximide treatment (250 ug/ml) of plasmodia produced invaginations of mitochondrial membranes and some circularization of cristae. (niu.edu)
Rats2
- To investigate how GAP43 expression (GAP43-ir) correlates with MFS, we assessed the intensity (densitometry) and extension (width) of GAP43-ir in the inner molecular layer of the dentate gyrus (IML) of rats subject to status epilepticus induced by pilocarpine (Pilo), previously injected or not with cycloheximide (CHX), which has been shown to inhibit MFS. (elsevier.com)
- Longo, B , Vezzani, A & Mello, LE 2005, ' Growth-associated protein 43 expression in hippocampal molecular layer of chronic epileptic rats treated with cycloheximide ', Epilepsia , vol. 46, no. (elsevier.com)
Chase1
- The measurement of half-life of the protein was done using the cycloheximide chase assay based on the method described earlier [37]. (thefreedictionary.com)
Resistant1
- These effects are resistant to cycloheximide but are inhibited by a dominant-negative ISRE-binding protein, indicating that vIRF acts together with a cellular cofactor at the PRF element to directly transactivate MYC . (pnas.org)
Proteins1
- The results indicate that the short onset of microsomal RNase reduction produced by cycloheximide may be due to a more rapid turnover of this enzyme in comparison with other microsomal proteins. (aspetjournals.org)
Apoptotic3
- Cycloheximide inhibits binding of Aminoacyl-tRNA to the ribosomes, Peptidyltransferase, detachment of deacylated tRNA, and translocation of Aminoacyl-tRNA, resulting in cell growth arrest and apoptotic cell death. (carlroth.com)
- Purpose: Mitomycin C (MMC) and cycloheximide (CHX) are known for their apoptotic and antitumor activity. (ac.rs)
- Anti-apoptotic actions of cycloheximide: Blockade of programmed cell death or induction of programmed cell life? (elsevier.com)