AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
CycloheximideProtein Synthesis InhibitorsDactinomycinPuromycinProtein BiosynthesisRNA, MessengerCells, CulturedEmetineCell LineKineticsTranscription, GeneticLeucineEnzyme InductionDexamethasoneAnisomycinPactamycinTime FactorsGene Expression RegulationNucleic Acid Synthesis InhibitorsDepression, ChemicalTritiumTumor Cells, CulturedBlotting, NorthernRNAChloramphenicolTetradecanoylphorbol AcetateUridineFibroblastsCell NucleusApoptosisDose-Response Relationship, DrugColchicineAmanitinsMolecular Sequence DataBase SequenceLiverDEAE-DextranCulture MediaBucladesineOrnithine DecarboxylasePeptide BiosynthesisInterleukin-1Biological TransportCarbon IsotopesTumor Necrosis Factor-alphaCyclic AMPDeoxyadenosinesPolyribosomesL Cells (Cell Line)Cytochalasin BStimulation, ChemicalHalf-LifeDNAMethionineRats, Inbred StrainsEnzyme ActivationProteinsAntimetabolitesAminoisobutyric AcidsGene ExpressionEnzyme InhibitorsRibosomesDisulfotonCulture TechniquesCricetinae
Cycloheximide
Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
Protein Synthesis Inhibitors
Compounds which inhibit the synthesis of proteins. They are usually ANTI-BACTERIAL AGENTS or toxins. Mechanism of the action of inhibition includes the interruption of peptide-chain elongation, the blocking the A site of ribosomes, the misreading of the genetic code or the prevention of the attachment of oligosaccharide side chains to glycoproteins.
Dactinomycin
A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)
Puromycin
A cinnamamido ADENOSINE found in STREPTOMYCES alboniger. It inhibits protein synthesis by binding to RNA. It is an antineoplastic and antitrypanosomal agent and is used in research as an inhibitor of protein synthesis.
Protein Biosynthesis
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Emetine
The principal alkaloid of ipecac, from the ground roots of Uragoga (or Cephaelis) ipecacuanha or U. acuminata, of the Rubiaceae. It is used as an amebicide in many different preparations and may cause serious cardiac, hepatic, or renal damage and violent diarrhea and vomiting. Emetine inhibits protein synthesis in EUKARYOTIC CELLS but not PROKARYOTIC CELLS.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Kinetics
The rate dynamics in chemical or physical systems.
Transcription, Genetic
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Leucine
An essential branched-chain amino acid important for hemoglobin formation.
Enzyme Induction
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
Dexamethasone
An anti-inflammatory 9-fluoro-glucocorticoid.
Anisomycin
An antibiotic isolated from various Streptomyces species. It interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system.
Pactamycin
Antibiotic produced by Streptomyces pactum used as an antineoplastic agent. It is also used as a tool in biochemistry because it inhibits certain steps in protein synthesis.
Time Factors
Elements of limited time intervals, contributing to particular results or situations.
Gene Expression Regulation
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Nucleic Acid Synthesis Inhibitors
Compounds that inhibit cell production of DNA or RNA.
Depression, Chemical
The decrease in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
Tritium
Tumor Cells, Cultured
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Blotting, Northern
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Chloramphenicol
An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)
Tetradecanoylphorbol Acetate
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Uridine
Fibroblasts
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Dose-Response Relationship, Drug
The relationship between the dose of an administered drug and the response of the organism to the drug.
Colchicine
A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE).
Amanitins
Cyclic peptides extracted from carpophores of various mushroom species. They are potent inhibitors of RNA polymerases in most eukaryotic species, blocking the production of mRNA and protein synthesis. These peptides are important in the study of transcription. Alpha-amanitin is the main toxin from the species Amanitia phalloides, poisonous if ingested by humans or animals.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Liver
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
DEAE-Dextran
Used as a support for ion-exchange chromatography.
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Bucladesine
A cyclic nucleotide derivative that mimics the action of endogenous CYCLIC AMP and is capable of permeating the cell membrane. It has vasodilator properties and is used as a cardiac stimulant. (From Merck Index, 11th ed)
Ornithine Decarboxylase
A pyridoxal-phosphate protein, believed to be the rate-limiting compound in the biosynthesis of polyamines. It catalyzes the decarboxylation of ornithine to form putrescine, which is then linked to a propylamine moiety of decarboxylated S-adenosylmethionine to form spermidine.
Peptide Biosynthesis
The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.
Interleukin-1
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
Biological Transport
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Carbon Isotopes
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
Tumor Necrosis Factor-alpha
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Cyclic AMP
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Deoxyadenosines
Adenosine molecules which can be substituted in any position, but are lacking one hydroxyl group in the ribose part of the molecule.
Polyribosomes
A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
L Cells (Cell Line)
A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.
Cytochalasin B
A cytotoxic member of the CYTOCHALASINS.
Stimulation, Chemical
The increase in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.
Half-Life
The time it takes for a substance (drug, radioactive nuclide, or other) to lose half of its pharmacologic, physiologic, or radiologic activity.
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Methionine
A sulfur-containing essential L-amino acid that is important in many body functions.
Rats, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Enzyme Activation
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Antimetabolites
Drugs that are chemically similar to naturally occurring metabolites, but differ enough to interfere with normal metabolic pathways. (From AMA Drug Evaluations Annual, 1994, p2033)
Aminoisobutyric Acids
A group of compounds that are derivatives of the amino acid 2-amino-2-methylpropanoic acid.
Gene Expression
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Enzyme Inhibitors
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
Ribosomes
Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.
Disulfoton
An organothiophosphate insecticide.
Culture Techniques
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Cricetinae
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.

Bcl-2 and Bcl-XL serve an anti-inflammatory function in endothelial cells through inhibition of NF-kappaB. (1/5972)

To maintain the integrity of the vascular barrier, endothelial cells (EC) are resistant to cell death. The molecular basis of this resistance may be explained by the function of antiapoptotic genes such as bcl family members. Overexpression of Bcl-2 or Bcl-XL protects EC from tumor necrosis factor (TNF)-mediated apoptosis. In addition, Bcl-2 or Bcl-XL inhibits activation of NF-kappaB and thus upregulation of proinflammatory genes. Bcl-2-mediated inhibition of NF-kappaB in EC occurs upstream of IkappaBalpha degradation without affecting p65-mediated transactivation. Overexpression of bcl genes in EC does not affect other transcription factors. Using deletion mutants of Bcl-2, the NF-kappaB inhibitory function of Bcl-2 was mapped to bcl homology domains BH2 and BH4, whereas all BH domains were required for the antiapoptotic function. These data suggest that Bcl-2 and Bcl-XL belong to a cytoprotective response that counteracts proapoptotic and proinflammatory insults and restores the physiological anti-inflammatory phenotype to the EC. By inhibiting NF-kappaB without sensitizing the cells (as with IkappaBalpha) to TNF-mediated apoptosis, Bcl-2 and Bcl-XL are prime candidates for genetic engineering of EC in pathological conditions where EC loss and unfettered activation are undesirable.  (+info)

NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae. (2/5972)

A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. Molecular genetic analysis of NMD3 revealed that it is an essential gene required for stable 60S ribosomal subunits. Cells bearing a temperature-sensitive allele of NMD3 had decreased levels of 60S subunits at the nonpermissive temperature which resulted in the formation of half-mer polysomes. Pulse-chase analysis of rRNA biogenesis indicated that 25S rRNA was made and processed with kinetics similar to wild-type kinetics. However, the mature RNA was rapidly degraded, with a half-life of 4 min. Nmd3p fractionated as a cytoplasmic protein and sedimented in the position of free 60S subunits in sucrose gradients. These results suggest that Nmd3p is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. Putative orthologs of Nmd3p exist in Drosophila, in nematodes, and in archaebacteria but not in eubacteria. The Nmd3 protein sequence does not contain readily recognizable motifs of known function. However, these proteins all have an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization.  (+info)

delta-Aminolevulinate synthetases in the liver cytosol fraction and mitochondria of mice treated with allylisopropylacetamide and 3,5-dicarbethoxyl-1,4-dihydrocollidine. (3/5972)

Hepatic delta-aminolevulinate (ALA) synthetase was induced in mice by the administration of allylisopropylacetamide (AIA) and 3,5-dicarbethoxy-1,4-dihydrocollidine (DDC). In both cases, a significant amount of ALA synthetase accumulated in the liver cytosol fraction as well as in the mitochondria. The apparent molecular weight of the cytosol ALA synthetase was estimated to be 320,000 by gel filtration, but when the cytosol ALA synthetase was subjected to sucrose density gradient centrifugation, it showed a molecular weight of 110,000. In the mitochondria, there were two different sizes of ALA synthetase with molecular weights of 150,000 and 110,000, respectively; the larger enzyme was predominant in DDC-treated mice, whereas in AIA-treated mice and normal mice the enzyme existed mostly in the smaller form. When hemin was injected into mice pretreated with DDC, the molecular size of the mitochondrial ALA synthetase changed from 150,000 to 110,000. The half-life of ALA synthetase in the liver cytosol fraction was about 30 min in both the AIA-treated and DDC-treated mice. The half-life of the mitochondrial ALA synthetase in AIA-treated mice and normal mice was about 60 min, but in DDC-treated mice the half-life was as long as 150 min. The data suggest that the cytosol ALA synthetase of mouse liver is a protein complex with properties very similar to those of the cytosol ALA synthetase of rat liver, which has been shown to be composed of the enzyme active protein and two catalytically inactive binding proteins, and that ALA synthetase may be transferred from the liver cytosol fraction to the mitochondria with a size of about 150,000 daltons, followed by its conversion to enzyme with a molecular weight of 110,000 within the mitochondria. The process of intramitochondrial enzyme degradation seems to be affected in DDC-treated animals.  (+info)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (4/5972)

Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease.  (+info)

Expression of thrombospondin-1 in ischemia-induced retinal neovascularization. (5/5972)

Thrombospondin-1 is an extracellular matrix protein that inhibits endothelial cell proliferation, migration, and angiogenesis. This study was performed to investigate the role of thrombospondin-1 in ischemic retinal neovascularization. In a murine model of retinal neovascularization, thrombospondin-1 mRNA was increased from postnatal day 13 (P13), with a threefold peak response observed on P15, corresponding to the time of development of retinal neovascularization. Prominent expression of thrombospondin-1 was observed in neovascular cells, specifically, cells adjacent to the area of nonperfusion. It has been suggested that vascular endothelial growth factor (VEGF) plays a major role in ischemia-induced retinal neovascularization of this model, so we studied the effects of VEGF on thrombospondin-1 expression. In bovine retinal microcapillary endothelial cells, VEGF induced a biphasic response of thrombospondin-1 expression; VEGF decreased thrombospondin-1 mRNA 0.41-fold after 4 hours, whereas it increased, with a threefold peak response, after 24 hours. VEGF-induced endothelial cell proliferation was completely inhibited by exogenous thrombospondin-1 and increased by 37.5% with anti-thrombospondin-1 antibody. The present findings suggest that, in the ischemic retina, retinal neovascular cells increase thrombospondin-1 expression, and VEGF may stimulate endogenous thrombospondin-1 induction, which inhibits endothelial cell growth. VEGF-mediated thrombospondin-1 induction in ischemia-induced angiogenesis may be a negative feedback mechanism.  (+info)

5'-Nucleotidase activity of mouse peritoneal macrophages. II. Cellular distribution and effects of endocytosis. (6/5972)

The diazonium salt of sulfanilic acid (DASA) can inactivate about 80% of the total 5'-nucleotidase of viable macrophages. The remaining 20% can be inactivated if the cells are first lysed in detergent, and presumably represents an intracellular pool of 5'-nucleotidase. The bulk of this pool may represent cytoplasmic vesicles derived from plasma membrane by endocytosis. This internal compartment is expanded up to threefold immediately after the cells have ingested a large latex load. This is consistent with previous observations on the internalization of 5'-nucleotidase in latex phagosomes. In latex-filled cells this intracellular pool of enzyme is inactivated over a few hours, and the cells then slowly increase their enzyme activity to nearly normal levels. However, 24 h after latex ingestion the metabolism of 5'-nucleotidase in these recovered cells is abnormal, as the rate of enzyme degradation is about twice the normal rate, and the DASA-insensitive enzyme pool in these cells is strikingly diminished. This may reflect effects of the accumulated indigestible particles on the fate of incoming pinocytic vesicles or on newly synthesized plasma membrane precursor. Another endocytic stimulus, concanavalin A, also reduces the total cell 5'-nucleotidase activity. This effect, which is time and temperature dependent, can be prevented by the competitive sugar alpha-methyl mannose. The concanavalin A inhibition can be reversed in the absence of new protein synthesis or in cells cultivated in serum-free conditions. It is not known whether the effect of concanavalin A on 5'-nucleotidase depends upon the interiorizaiton of plasma membrane or is strictly associated with events at the cell surface.  (+info)

CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo. (7/5972)

cAMP activated insertion of the cystic fibrosis transmembrane conductance regulator (CFTR) channels from endosomes to the apical plasma membrane has been hypothesized to regulate surface expression and CFTR function although the physiologic relevance of this remains unclear. We previously identified a subpopulation of small intestinal villus epithelial cells or CFTR high expressor (CHE) cells possessing very high levels of apical membrane CFTR in association with a prominent subapical vesicular pool of CFTR. We have examined the subcellular redistribution of CFTR in duodenal CHE cells in vivo in response to the cAMP activated secretagogue vasoactive intestinal peptide (VIP). Using anti-CFTR antibodies against the C terminus of rodent CFTR and indirect immunofluorescence, we show by quantitative confocal microscopy that CFTR rapidly redistributes from the cytoplasm to the apical surface upon cAMP stimulation by VIP and returns to the cytoplasm upon removal of VIP stimulation of intracellular cAMP levels. Using ultrastructural and confocal immunofluorescence examination in the presence or absence of cycloheximide, we also show that redistribution was not dependent on new protein synthesis, changes in endocytosis, or rearrangement of the apical cytoskeleton. These observations suggest that physiologic cAMP activated apical membrane insertion and recycling of CFTR channels in normal CFTR expressing epithelia contributes to the in vivo regulation of CFTR mediated anion transport.  (+info)

Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (8/5972)

A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided.  (+info)

The effects of anisomycin (a protein synthesis inhibitor) on spatial learning and memory in CA1 region of rats hippocampusThe effects of anisomycin (a protein synthesis inhibitor) on spatial learning and memory in CA1 region of rats hippocampus
Studies were performed to investigate whether electrically-induced long-term depression (LTD) within rat hippocampal slices in vitro shares any common cellular features with LTD in the intact animal, with particular emphasis being placed on mechanisms required for its late maintenance. Our initial studies have led to the development of stimulation protocols which are able to reliably produce different forms of LTD. Depending on the induction protocol applied, we are able to demonstrate a transient protein synthesis-independent early-LTD with a duration of up to 3-4 h, together with a de novo protein synthesis-dependent late-LTD lasting for at least 8 h. Furthermore, we are able to show input-specific LTD within the CA1 region, with expression shown only by those synapses specifically stimulated by a low-frequency protocol. These studies are important pre-requisites to investigate mechanisms of synaptic tagging and late-associativity during LTD ...
http://anisomycin.noneto.com/a3.htm
HTLV receptor is an early T-cell activation marker whose expression requires de novo protein synthesis | Blood | American...HTLV receptor is an early T-cell activation marker whose expression requires de novo protein synthesis | Blood | American...
Expression of the HTLV receptor was strictly dependent on de novo protein synthesis. It has been shown that prior to T-lymphocyte proliferation, there is a 7- to 10-fold increase in protein synthesis and a 30- to 40-fold augmentation in mRNA synthesis.33This pre-proliferation characteristic appears to be a particularity of T lymphocytes and is not observed in other cell types. The required pre-proliferation burst in mRNA/protein synthesis likely results from the extremely low metabolic rate of resting G0T lymphocytes, which make up the vast majority of the circulating T-lymphocyte pool. Thus, it is intriguing to speculate that it is this low metabolic rate that accounts for the fact that T lymphocytes are the first and only cell type, identified to date, which do not constitutively express the HTLV receptor. Notably, Wodarz and Bangham have recently used a mathematical model to suggest that the rate of evolution of HTLV-1 is limited by the restricted availability of activated uninfected T ...
https://ashpublications.org/blood/article/101/5/1913/106598/The-HTLV-receptor-is-an-early-T-cell-activation
Prevalent deficiency in tumor cells of cycloheximide-induced cycle arr by E E. Medrano and A B. PardeePrevalent deficiency in tumor cells of cycloheximide-induced cycle arr by E E. Medrano and A B. Pardee
Medrano, E E. and Pardee, A B., Prevalent deficiency in tumor cells of cycloheximide-induced cycle arrest. (1980). Subject Strain Bibliography 1980. 3388 ...
https://mouseion.jax.org/ssbb1980/3388/
Export Data and Price of Cycloheximide | www.eximpulse.comExport Data and Price of Cycloheximide | www.eximpulse.com
Export Data And Price Of Cycloheximide , www.eximpulse.com Eximpulse Services is the place where you can find the recent and updated Trade intelligence report of Cycloheximide Export Data. Whole information is based on updated Export shipment data of Indian Customs. All the compilation is done on the basis of All India ports data and has been done on daily basis. This helps you to get all India Cycloheximide Export data. You can find previous two days Cycloheximide Export data on Eximpulse Services. Cycloheximide Export data can be useful in different kind of analysis such as: Export price, Quantity, market scenarios, Price trends, Duty optimization and many more. Some Sample Shipment records for Cycloheximide Export Data of India are mentioned above. Further for Free sample and pricing of detailed reports contact on [email protected] Data post 2012 as per Notification No.18/2012 - Customs(N.T.) and does not have names of Indian companies and Foreign Companies.. ...
https://www.eximpulse.com/export-product-Cycloheximide.htm
Plus itPlus it
We have investigated the effect of chemotherapeutic and DNA damaging agents on binding of the tumor suppressor phosphoprotein p53 to its consensus DNA sequence. Activation of p53-DNA binding was seen for treatment with radiation, hydrogen peroxide, actinomycin D, Adriamycin, etoposide, camptothecin, 5-fluorouracil, mitomycin C, and cisplatin. These results showed that DNA strand breaks were sufficient to lead to increased levels of p53. The protein synthesis inhibitor cycloheximide blocks the increase in p53 following DNA damage. The increase in p53 activation in camptothecin treated cells may result, at least in part, from an increased half-life of the protein and consequent increases in intracellular protein concentration.. ...
http://cancerres.aacrjournals.org/content/53/10/2212
McClean: Cycloheximide Stock Solution - OpenWetWareMcClean: Cycloheximide Stock Solution - OpenWetWare
Weigh out the appropriate amount of cycloheximide and add it to the appropriate amount of DMSO to get a 50mg/ml solution. Use the Botstein labs balance as it is much more accurate than ours. It seems to make the most sense to aim for about 100mg (but if it is slightly over or under that is fine) and then put this in a 2ml eppendorf or similar tube (you can use a 15ml conical if you are going to make a larger volume) and add the appropriate amount of DMSO to this based on what you weighed out. Cycloheximide is bad for you (see below) so be careful with it! ...
https://openwetware.org/wiki/McClean:_Cycloheximide_Stock_Solution
Protective effect of cycloheximide upon protein synthesis by l5178y ce by J E. Fuhr, M Overton et al.Protective effect of cycloheximide upon protein synthesis by l5178y ce by J E. Fuhr, M Overton et al.
Fuhr, J E.; Overton, M; and Leisy, M, Protective effect of cycloheximide upon protein synthesis by l5178y cells exposed to hyperthermia. (1974). Subject Strain Bibliography 1974. 2356 ...
https://mouseion.jax.org/ssbb1974/2356/
cycloheximide | VWRcycloheximide | VWR
Learn more about cycloheximide. We enable science by offering product choice, services, process excellence and our people make it happen.
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Gene expressionGene expression
Process of gene expression entails plasmid DNA or mRNA delivery to specific cells and is a technique routinely utilized in labs to study a gene of interest or a signaling pathway. Gene expression usually allows transient protein production, but in some cases stable integration into a genome and continuous protein production over time is required.
https://www.polyplus-transfection.com/application/gene-expression/
Effect of protein synthesis inhibitors on the hormonal induction of alanine and aspartate aminotransferases in the liver of...Effect of protein synthesis inhibitors on the hormonal induction of alanine and aspartate aminotransferases in the liver of...
Reference: Polikarpova L.I., Effect of protein synthesis inhibitors on the hormonal induction of alanine and aspartate aminotransferases in the liver of sexually mature male rats, Voprosy meditsinskoi khimii, 1974, vol: 20(2), 215-217 ...
http://pbmc.ibmc.msk.ru/index.php/en/article/PBMC-1974-20-2-215-en
Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities | Journal of Cell ScienceCycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities | Journal of Cell Science
Fertilization of metaphase II-arrested mouse eggs results in resumption of meiosis and a decrease in both cdc2/cyclin B kinase and MAP kinase activities; the decrease in cdc2/cyclin B kinase activity precedes the decrease in MAP kinase activity. Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the fertilization-induced Ca2+ transient. However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase activities that are intimately associated with resumption of meiosis. We report that cycloheximide-treated mouse eggs manifest similar temporal changes in the decrease in both cdc2/cyclin B kinase and MAP kinase activities that occur following fertilization, although cortical granule exocytosis is not stimulated. The decrease in cdc2/cyclin B kinase activity, however, does not seem to be required for the decrease in MAP kinase activity, since the decrease in MAP kinase activity ...
http://jcs.biologists.org/content/109/4/739
Plouhinec JL et al. (2014), Pax3 and Zic1 trigger the early neural crest ge... - Xenbase PaperPlouhinec JL et al. (2014), Pax3 and Zic1 trigger the early neural crest ge... - Xenbase Paper
Fig. 5. Identification of Pax3 binding sites on Pax3 promoters in vitro (A) Pax3, but not Zic1 alone, can trigger synthesis of the endogenous Pax3 transcript (primers in 3UTR, not amplifying the inducible form) either in absence of the translation inhibitor cycloheximide (induction is 5-fold compared to uninjected animal caps) or in the presence of cycloheximide (induction is 31-fold, compared to cycloheximide-treated uninjected animal caps). Endogenous pax3 expression level in a stage 11 whole embryo is set at a relative value of 1 unit. After co-injection of Pax3 and Zic1, endogenous pax3 was activated as an immediate-early target as well (77-fold compared to uninjected animal caps; and, in presence of cycloheximide, 16-fold when compared to cycloheximide-treated uninjected animal caps). (B) Location of the ECR containing Pax3 putative binding site in the genomic sequence upstream of the Pax3 TSS. (C) Pax3 binds specifically to the motif identified in the pax3 promoter ECR: an electrophoretic ...
http://www.xenbase.org/literature/article.do?method=display&articleId=48413
Hypoxic neuronal necrosis: Protein synthesis-independent activation of a cell death program | Proceedings of the National...Hypoxic neuronal necrosis: Protein synthesis-independent activation of a cell death program | Proceedings of the National...
In DG cultures exposed to 10 mM NaCN for 45 min, ≈90% of neurons (identified by immunoreactivity against NeuN, a neuronal marker) died 24 h after hypoxia, because they stained with propidium iodide. Survival of GFAP-immunoreactive glia was not affected (data not shown). Combining the same cyanide treatment with glucose deprivation (ischemia) had very similar effects on the neurons. By EM, many hypoxic neurons showed early swelling of mitochondria and endoplasmic reticulum, in the presence of an intact nucleus (Fig. 1B), and evolved later toward necrosis with severe cytoplasmic swelling and membrane rupture (Fig. 1C). This finding was in sharp contrast to staurosporine-treated apoptotic neurons, which displayed nuclear breakdown into large, round masses of chromatin (Fig. 1D). Hypoxic necrosis evolved rapidly: we observed a progressive loss of the mitochondrial membrane potential Δψ within a few minutes of cyanide application (Fig. 2 A-D). This Δψ decay was always synchronous with release ...
http://www.pnas.org/content/100/5/2825
Search Results for oxytocin | joeSearch Results for oxytocin | joe
Gonadotrophin-releasing activity of oxytocin has previously been demonstrated in vitro and in vivo. This study investigated whether oxytocin is also able to induce LH accumulation in pituitary cells. Following trypsin digestion and mechanical dispersion, pituitary cells from female rats were incubated with oxytocin (100 nmol/l) for 24 h. LH release stimulated by oxytocin increased (P , 0·001) progressively during the incubation indicating a different secretory pattern from the more rapid but less sustained secretion stimulated by gonadotrophin-releasing hormone. Oxytocin also enhanced (P , 0·01) total LH accumulation in the incubation system (released plus cell contents) which was apparent after 7-11 h of stimulation. The release of LH stimulated by oxytocin was reduced by the protein synthesis inhibitor cycloheximide (10 μmol/l). However, cycloheximide did not completely block oxytocin-stimulated LH release; there remained some LH release above that seen in non-stimulated controls (P , ...
https://joe.bioscientifica.com/search?access=all&page=7&pageSize=10&q=%22oxytocin%22&sort=relevance
Plus itPlus it
Treatment with the protein synthesis inhibitor cycloheximide (CHX) or silencing of the core component of the NMD machinery, Upf-1 (also known as Rent1), are widely used to determine if transcripts are subject to NMD (Montfort et al., 2006). CHX is an indirect NMD inhibitor since NMD activation is posttranscriptional, but translation-dependent, and ribosomal association is required during the pioneer round of translation. Upf-1 silencing is believed to produce direct suppression of NMD. The RNA helicase, Upf-1, is a central effector of NMD that links the translation-termination event to the assembly of a surveillance complex. Upf2 and Upf3 are believed to recruit and activate Upf1 on NMD substrates (Lykke-Andersen et al., 2000). Although the discrimination of PTCs from normal termination codons and the molecular links that trigger NMD are still unclear, it is well established that the core components of the NMD machinery are the conserved proteins, Upf-1, Upf-2 and Upf-3. PTC-containing mRNAs ...
http://www.jneurosci.org/content/29/9/2833
Search Results | Journal of Cell Biology | Rockefeller University PressSearch Results | Journal of Cell Biology | Rockefeller University Press
When cultivated on substrates that prevent cell adhesion (the polymer polyhydroxyethylmethacrylate, bovine serum albumin, and Teflon), human endothelial cells (EC) rapidly lost viability with a half-life of approximately 10 h. Dying EC showed the morphological and biochemical characteristics of apoptosis. The apoptotic process of suspended EC was delayed by the protein synthesis inhibitor cycloheximide. To obtain information as to the mechanism involved in the apoptosis of suspended EC, we investigated whether adhesion to matrix proteins or integrin occupancy in EC retaining a round shape may affect EC suicide. EC bound to low coating concentration of either fibronectin or vitronectin, retaining a round shape and failing to organize actin microfilaments, underwent to rapid cell death; by contrast, cells on high substrate concentrations became flattened, showed actin microfilament organization, and retained viability. Addition of saturating amounts of soluble vitronectin to suspended round-shaped ...
https://rupress.org/jcb/search-results?f_Authors=M+Sironi
Cycloheximide - Creative PeptidesCycloheximide - Creative Peptides
Cycloheximide is an antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. Cycloheximide acts by inhibiting elongation during protein synthesis.
https://www.creative-peptides.com/product/cycloheximide-item-z10-101-163-34595.html
A) Western blot demonstrating cycloheximide effects of | Open-iA) Western blot demonstrating cycloheximide effects of | Open-i
A) Western blot demonstrating cycloheximide effects of Wnt-3A mediated accumulation of β-catenin. NCCIT cells were stimulated for 4 hours with CCM (C) or Wnt-3
https://openi.nlm.nih.gov/detailedresult.php?img=PMC117803_1471-213X-2-8-4&req=4
Protein Synthesis Inhibitors | FlashcardsProtein Synthesis Inhibitors | Flashcards
Take a look at our interactive learning Flashcards about Protein Synthesis Inhibitors, or create your own Flashcards using our free cloud based Flashcard maker.
https://www.goconqr.com/flashcard/89322/protein-synthesis-inhibitors
TNFα-induced lysosomal membrane permeability is downstream of MOMP and triggered by caspase-mediated NDUFS1 cleavage and ROS...TNFα-induced lysosomal membrane permeability is downstream of MOMP and triggered by caspase-mediated NDUFS1 cleavage and ROS...
TNFα, the founding member of the TNF-superfamily, mediates both necroptotic and apoptotic cell death (Walczak, 2011; Micheau and Tschopp, 2003; Andrew, 2004; Wang et al., 2008; Kroemer et al., 2007; Degterev and Yuan, 2008, He et al., 2009; Zhang et al., 2009). Although many investigations have been conducted over the last few years the detailed mechanisms of these death signaling pathways are still not completely known. This particularly concerns our understanding about where ROS are produced intracellularly, how ROS and LMP are mechanistically linked and how both processes contribute to TNFα-induced apoptosis (Boya and Kroemer, 2008; Shen and Pervaiz, 2006; Zdolsek et al., 1993; Brunk et al., 1995). Our study here bridges these missing gaps. We identified that TNFα/CHX-induced LMP is not an apoptosis initiating, but amplifying process downstream of MOMP, and it is triggered by mitochondrial ROS generated as the result of a caspase-3-mediated cleavage of the p75 NDUFS1 subunit of complex I. ...
http://jcs.biologists.org/content/126/17/4015
SynPharm: A New Annexe to the Guide to PHARMACOLOGY | guidetopharmacology blogSynPharm: A New Annexe to the Guide to PHARMACOLOGY | guidetopharmacology blog
We have created a new sister database to the main Guide to PHARMACOLOGY (GtoPdb) - SynPharm, a database of drug-responsive protein sequences. Each sequence in SynPharm is derived from a GtoPdb interaction. In each case we have identified the continuous protein sequence within the receptor chain that facilitates that interaction, and provided structural, visual, spatial…
https://blog.guidetopharmacology.org/2016/10/04/synpharm-a-new-annexe-to-the-guide-to-pharmacology/
Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation...Effects of cycloheximide on thermotolerance expression, heat shock protein synthesis, and heat shock protein mRNA accumulation...
A single hyperthermic exposure can render cells transiently resistant to subsequent high temperature stresses. Treatment of rat embryonic fibroblasts with cycloheximide for 6 h after a 20-min interval at 45 degrees C inhibits protein synthesis, including heat shock protein (hsp) synthesis, and results in an accumulation of hsp 70 mRNA, but has no effect on subsequent survival responses to 45 degrees C hyperthermia. hsp 70 mRNA levels decreased within 1 h after removal of cycloheximide but then appeared to stabilize during the next 2 h (3 h after drug removal and 9 h after heat shock). hsp 70 mRNA accumulation could be further increased by a second heat shock at 45 degrees C for 20 min 6 h after the first hyperthermic exposure in cycloheximide-treated cells. Both normal protein and hsp synthesis appeared increased during the 6-h interval after hyperthermia in cultures which received two exposures to 45 degrees C for 20 min compared with those which received only one treatment. No increased hsp ...
https://mcb.asm.org/content/6/4/1088
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106. Abstract Protoplasts of Boergesenia forbesii (Hanvey) were treated with inhibitors of protein synthesis in order to investigate their effects on cellulose synthesis. Cellulose synthesis was reversibly inhibited by 10 M cycloheximide as assayed by fluorescence microscopy of Tinopal binding to cellulose. Freeze fracture and image analysis of cycloheximide-treated cells indicated a reduction in the number of intramembrane particles; however, the terminal synthesizing complexes remained at all times. Treatment with 10 M actinomycin D, when applied during the first hour of protoplast formation, irreversibly inhibits cellulose synthesis and terminal complex formation. De novo protein synthesis is required for cell wall regeneration by protoplasts. The data suggest that the structural subunits visualized in the terminal complex do not undergo signifi-cant turnover, but that there may exist an essential proteinaceous component of cellulose synthesis which must be continually renewed ...
http://www.botany.utexas.edu/facstaff/facpages/mbrown/papers/106.htm
Cycloheximide - WikipediaCycloheximide - Wikipedia
Cycloheximide is a eukaryote protein synthesis inhibitor, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome), thus blocking translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.[1] Due to significant toxic side effects, including DNA damage, teratogenesis, and other reproductive effects (including birth defects and toxicity to sperm[2]), cycloheximide is generally used only in in vitro research applications, and is not suitable for human use as a therapeutic compound. Although it has been used as a fungicide in agricultural applications, this application is now decreasing as the health risks have become ...
https://en.wikipedia.org/wiki/Cycloheximide
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Memory can be divided according to different temporal phases: acquisition, consolidation, and retrieval. The consolidation phase is divided into molecular consolidation (range of hours after acquisition) and system consolidation (range of weeks and months after acquisition) (Dudai, 2004). Molecular consolidation is thought to be a protein synthesis-dependent process (Alberini, 2008; Costa-Mattioli et al., 2009). The clear effect of protein synthesis inhibitors on long-term memory was studied extensively mainly in the context of late-phase long-term potentiation (LTP) (Abraham and Williams, 2008). Similar molecular mechanisms were proposed for maintenance of long-term synaptic modifications and learning processes (Davis and Squire, 1984; Costa-Mattioli et al., 2009). However, long-term memory is not supported only by modulation of synaptic strength; modifications in intrinsic neuronal properties also subserve learning-related behavioral changes (Saar and Barkai, 2003; Zhang and Linden, 2003; ...
http://www.jneurosci.org/content/30/12/4338
The Effect of Cycloheximide on the Replication of Measles Virus | Microbiology SocietyThe Effect of Cycloheximide on the Replication of Measles Virus | Microbiology Society
Summary Treatment of measles virus-infected cells with cycloheximide results in a three-fold increase of 3H-uridine incorporation into the 12 to 36S mRNA species and in the inhibition of genomic 50S RNA synthesis. Consistent with these observations was the finding of a build-up of polyribosomes but an absence of nucleocapsids in the infected cells. These results suggest that measles virus RNA replication, but not transcription, is dependent upon active protein synthesis.
https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-35-3-579
Constitutive Nuclear Factor κB Activity Is Required for Survival of Activated B Cell-like Diffuse Large B Cell Lymphoma Cells ...Constitutive Nuclear Factor κB Activity Is Required for Survival of Activated B Cell-like Diffuse Large B Cell Lymphoma Cells ...
This conclusion was supported by studies of IκBα protein turnover in the DLBCL cell lines. Because IκBα transcription is itself positively regulated by NF-κB activity (37, 38), the steady-state level of IκBα protein does not necessarily reflect the rate of phosphorylation by IKK and subsequent degradation. Indeed, full-length IκBα protein is found at comparable levels in our untreated cell lines (Fig. 3 A). However, when ABC DLBCL cell lines were treated with cycloheximide (CHX) to block new protein synthesis, the level of IκBα declined rapidly through phosphorylation and degradation (Fig. 3 A). By contrast, the IκBα level in SUDHL-6 was significantly more stable upon CHX treatment unless the cells were also stimulated by BCR cross-linking or by PMA and ionomycin treatment (Fig. 3 A). Lactacystin, a drug that inhibits degradation of ubiquitinated IκBα by proteasomes, prevented the CHX-induced loss of IκBα in resting ABC DLBCL cell lines and in stimulated SUDHL-6 cells (Fig. 3 ...
https://rupress.org/jem/article/194/12/1861/39409/Constitutive-Nuclear-Factor-B-Activity-Is-Required
Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response...Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response...
TY - JOUR. T1 - Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo. AU - Hess, Patrick. AU - Meenakshi, T.. AU - Chan, Guy Chiu Kai. AU - Carlstedt-Duke, Jan. AU - Gustafsson, Jan Åke. AU - Payvar, Farhang. PY - 1990/4. Y1 - 1990/4. N2 - We have identified and characterized a 206-base-pair region downstream from rat α2u-globulin promoter that specifically mediates a delayed secondary response to glucocorticoids. Unlike positive primary glucocorticoid response elements (GREs), this regulatory element, termed delayed sGRE, dictates an inductive process preceded by a time lag of several hours and blocked by the protein synthesis inhibitor cycloheximide. Reminiscent of GREs and negative GREs (nGREs), a delayed sGRE confers hormonal regulation upon a linked heterologous promoter from a downstream position with respect to transcription start site and, remarkably, also interacts selectively with ...
https://scholars.houstonmethodist.org/en/publications/purified-glucocorticoid-receptors-bind-selectively-in-vitro-to-a--2
SR9243 | CAS#1613028-81-1 | LXR inverse agonist | MedKooSR9243 | CAS#1613028-81-1 | LXR inverse agonist | MedKoo
SR9243 is a potent and selective LXR inverse agonist. SR9243 kills cancer cells by inhibiting lipid production and the Warburg effect. SR9243 induces cell death in multiple types of cancer and does not cause the side effects that have derailed previous attempts to target these processes. In cancer cells, SR9243 significantly inhibited the Warburg effect and lipogenesis by reducing glycolytic and lipogenic gene expression. SR9243 induced apoptosis in tumors without inducing weight loss, hepatotoxicity, or inflammation. Malignant cells exhibit aerobic glycolysis (the Warburg effect) and become dependent on de novo lipogenesis, which sustains rapid proliferation and resistance to cellular stress.
http://www.medkoo.com/products/6856
Cycloheximide, Crystalline | Krackeler Scientific, Inc.Cycloheximide, Crystalline | Krackeler Scientific, Inc.
We are currently working diligently to update our website pricing information for the New Year. If you place an order, you will be acknowledged with any corrected pricing. If youd like the most current information sooner, please dont hesitate to drop us an email or give us a call and wed be happy to assist. Thank you for your patience while we are updating.. 800-334- ...
https://www.krackeler.com/catalog/product/1163/Cycloheximide-Crystalline
Anisomycin | AbcamAnisomycin | Abcam
Buy Anisomycin (CAS 22862-76-6), a protein synthesis inhibitor. Join researchers using high quality Anisomycin from Abcam and achieve your mission, faster.
http://www.abcam.com/anisomycin-ab120495.html
Anisomycin | Purity |98% | CAS 22862-76-6 | Alomone LabsAnisomycin | Purity |98% | CAS 22862-76-6 | Alomone Labs
High purity Anisomycin (#A-520) is a protein synthesis inhibitor from Alomone Labs. A natural & biologically active compound. New lots are biologically tested. Lyophilized. Free samples available. Global shipping at room temperature. Join the thousands of researchers using our products.
https://www.alomone.com/p/anisomycin/A-520?b=170
Personal induction planner
Record your induction journey here - identify your ongoing and emerging needs, and reflect on your induction processes as a beginning teacher.
https://education.nsw.gov.au/teaching-and-learning/professional-learning/teacher-quality-and-accreditation/strong-start-great-teachers/orientation/personal-induction-planner
N-methyl-D-aspartate receptor-mediated neuroprotection in cerebellar granule cells requires new RNA and protein synthesis | PNASN-methyl-D-aspartate receptor-mediated neuroprotection in cerebellar granule cells requires new RNA and protein synthesis | PNAS
Cerebellar granule cells are susceptible to the excitotoxin glutamate, which acts at N-methyl-D-aspartate (NMDA) receptors, as well as the neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+), the active cytotoxic metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Paradoxically, preincubation of cultured cerebellar granule cells with low concentrations of NMDA or glutamate markedly antagonizes the neurotoxicity resulting from subsequent exposure to toxic concentrations of either MPP+ or glutamate. The neuroprotective effects of NMDA and glutamate against MPP+ toxicity are observed at agonist concentrations as low as 1 microM, are blocked by specific NMDA receptor antagonists, and require at least 30 min to develop fully. Moreover, NMDA receptor-mediated neuroprotection is prevented by the RNA synthesis inhibitor actinomycin D or the protein synthesis inhibitor cycloheximide. Thus, in cerebellar granule cells activation of NMDA receptors by glutamate can result in either ...
https://www.pnas.org/content/89/14/6555?ijkey=f7b49354bd599adac24a2b8de34700a1869c12cd&keytype2=tf_ipsecsha
Download Free Protein Synthesis Like A Banana Split Answer Key, High quality vector files
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https://www.findanswerkey.com/answer/protein-synthesis-like-a-banana-split-answer-key/
Protein Synthesis Worksheet. Worksheets. Doriandnimo Thousands of Printable Activities
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Novel cycloheximide derivatives with antimicrobial activity against Legionella pneumophilaNovel cycloheximide derivatives with antimicrobial activity against Legionella pneumophila
Rasch et al. recently published an article describing antimicrobial properties of cycloheximide derivatives with adamantyl moieties against Legionella pneumophila. Ten different cycloheximide derivatives were synthesized in this study, and five were found to inhibit L. pneumophila growth at a concentration of 30 uM or 40 uM. Interestingly, several other clinically significant Gram-positive and Gram-negative pathogens were not susceptible to these derivatives which suggests a very narrow spectrum of activity which may be limited specifically to L. pneumophila ...
https://www.toku-e.com/Novel-cycloheximide-derivatives-with-antimicrobial-activity-against-Legionella-pneumophila.aspx
S propranolol synthesis - How to take dapoxetine 60
SABONNER; Plus de 88 millions dutilisateurs sur POF se réunissent pour se connecter, flirter, et partager avec chacun, ce qui donne plus de 10 millions de.The page you are looking for is no longer available: Comparative Study of Propranolol hydrochloride Release from Matrix Tablets with Kollidon®SR or Hydroxy Propyl.Propranolol has been used for MYOCARDIAL INFARCTION; ARRHYTHMIA; ANGINA PECTORIS; HYPERTENSION;. ATC code(s) propranolol and other combinations [ATC Code].mechanism(s). Materials and. oxide and cyclic guanosine monophosphate synthesis inhibitors on the extract effects were evaluated. Propranolol and N.Il sagit de choix des ouvrages, choix des genres, choix des textes, choix dauteurs en fonction du contexte socioculturel et politique de lapprenant.Propranolol Tablets Ip 40 Mg, Propranolol Generic And Trade Name. over the counter propranolol, propranolol urine drug test.. Titre du document / Document title Synthesis and chromatographic separation of the glucuronides of (R)- and ...
http://www.huntingnamibia.net/goxuz/s-propranolol-synthesis-629.php
Impact of Micro and Macroporous TFF Membranes on Product Sieving and Chromatography Loading for Perfusion Cell Culture | Aspen...
Bioprocess intensification can be achieved through high cell density perfusion cell culture with continuous protein capture integration. Protein passage and cell retention are commonly accomplished using tangential flow filtration systems consisting of microporous membranes. Significant challenges, including low efficiency and decaying product sieving over time, are commonly observed in these cell retention devices. Here, we demonstrate that a macroporous membrane overcomes the product sieving challenges when comparing to several other membrane chemistries and pore sizes within the microporous range. Learn More. ...
https://aspenxchange.com/2020/01/10/impact-of-micro-and-macroporous-tff-membranes-on-product-sieving-and-chromatography-loading-for-perfusion-cell-culture/
Protein Synthesis | Your Article Library
The mechanism of protein synthesis on 70S ribosomes includes the following stages: 1. The transcription: The process of protein synthesis is started by the uncoiling of strands of DNA molecule. One strand of DNA molecule acts as a template for the formation of mRNA. The mRNA is formed according to the triplet codes of DNA […]. ...
http://www.yourarticlelibrary.com/tag/articles-on-protein-synthesis
A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina | DevelopmentA propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina | Development
When DArcy Wentworth Thompsons On Growth and Form was published 100 years ago, it raised the question of how biological forms arise during development and across evolution. In light of the advances in molecular and cellular biology since then, a succinct modern view of the question states: how do genes encode geometry? Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales.. Read the Editorial by guest editors Thomas Lecuit and L. Mahadevan, as they provide a perspective on the influence of DArcy Thompsons work and an overview of the articles in this issue.. ...
http://dev.biologists.org/content/31/1/27
Turning Over a New Leaf. (I hope.) | Confessions of a Serial SwooperTurning Over a New Leaf. (I hope.) | Confessions of a Serial Swooper
Turning over a new leaf. Enough is enough. Since my wedding day, I have literally gained the amount of weight equal to the total weight of my now six-year-old son. As in, his weight today. Not the day he was born. Gross. So, Im back in the saddle. Two things need to happen, right?…
https://serialswooper.com/2011/09/23/turning-over-a-new-leaf-i-hope/?replytocom=1504
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Figure 5. A, HEC1A cells transfected with LacZ or p85α WT or E160* were treated with cycloheximide (CHX; left) for indicated duration or with MG132 (center) for 24 hours. Cells were then harvested for WB. PTEN levels were normalized to β-actin by densitometry (below). *, P , 0.05. Right, HEC1A cells were transfected with LacZ or WT or E160* in the absence or presence of ubiquitin (Ub) for 72 hours. Whole-cell lysates were collected for IP with anti-PTEN and then subjected to WB with anti-ubiquitin. PTEN protein levels were normalized prior to IP by using proportionally different amounts of lysates. B, left, HEC1A cells were transfected with LacZ or WT or its mutants for 72 hours and were collected for IP with PTEN and WB with anti-p85α. HEC1A cells were co-transfected with WT or increasing amount of E160*. Cell lysates were collected for IP (center) or WB (right top). Right bottom, transfected HEC1A cells were treated with CHX for the indicated time points and harvested for WB. C, cells were ...
http://cancerdiscovery.aacrjournals.org/content/2/8/750
chloramphenicol (thing) by witnie - Everything2.comchloramphenicol (thing) by witnie - Everything2.com
This antibiotic originating from the Gram+ actinomycete Streptomyces venezulenza has about the same type of activity as cycloheximide, but than with an ...
https://everything2.com/user/witnie/writeups/chloramphenicol
Transcriptional regulation of prolactin receptor gene expression by sodium butyrate in MCF-7 human breast cancer cells | Garvan...Transcriptional regulation of prolactin receptor gene expression by sodium butyrate in MCF-7 human breast cancer cells | Garvan...
The prolactin receptor (PRLR) mediates the diverse effects of prolactin, which in the mammary gland include the development of lobuloalveolar structures and increased tumor cell proliferation. Treatment of mammary carcinoma cells with the differentiating agent sodium butyrate (NaB) is known to reduce PRLR binding activity and PRLR gene expression, however the mechanism which mediates these changes is unknown, prompting this investigation. Using MCF-7 human breast cancer cells, assay of the rate of PRLR gene transcription by the nuclear run-on technique indicated that 3 mM NaB reduced PRLR gene transcription by 50% after 3 h of treatment and that this effect was maintained for at least 24 h. The protein synthesis inhibitor cycloheximide failed to abrogate this effect, which indicated that NaB did not require continuing protein synthesis to reduce the rate of PRLR transcription. Measurement of PRLR mRNA stability, using Northern blot analysis at various times after the inhibition of transcription with
https://www.garvan.org.au/research/publications/723
Biochemical characterization of spiromesifen and spirotetramat as lipid synthesis inhibitors on cotton leaf worm, Spodoptera...Biochemical characterization of spiromesifen and spirotetramat as lipid synthesis inhibitors on cotton leaf worm, Spodoptera...
Two lipid synthesis inhibitors compounds (spiromesifen SPM and spirotetramat SPT) were used against the second instar larvae of cotton leaf worm, Spodoptera littoralis under laboratory condition. Toxicity of both compounds was tested. Effect of these insecticides on total lipid also was determined. The obtained results showed that both compounds were moderately toxic against the second instar larvae of S. littoralis. The mortality percentages caused by the field rate of spiromesifen and spirotetramat were 51.7 and 60.0%, respectively. The lethal concentrations for 50% of tested insect population (LC50) were 170.1 and 42.2 ppm, respectively. The total lipid content was determined in treated larvae and compared with the untreated. The percentage of total lipid in spiromesifen- and spirotetramat-treated larvae was 2.17 and 2.21%, respectively, compared with 2.42% in untreated larvae. GC/MS also was used in identification of fatty acids composition of S. littoralis larvae. The results cleared that 13 fatty
https://bnrc.springeropen.com/articles/10.1186/s42269-019-0107-9
Apoptosis induced by mild hyperthermia in human and murine tumour cell lines: A study using electron microscopy and DNA gel...
Mild hyperthermia is known to enhance apoptosis in a range of normal and neoplastic cell populations. Studies of tumours previously shown to respond to heating in this manner might be expected to provide insights not only into the mechanism of hyperthermic cell killing, but also into the apoptotic process in general. In the present study, cell death induced by 43°C heating for 30 min in two human Burkitts lymphoma lines, BM 13674 and WW1, and in murine mastocytoma P‐815 × 2·1 was found to be exclusively apoptotic in type, identification being based on light and electron microscopic appearances and on the presence of internucleosomal cleavage of DNA into fragments that are multiples of 180-200 base pairs, which was demonstrated by agarose gel electrophoresis. The heat‐induced apoptosis was prevented by the presence of zinc sulphate, an inhibitor of the endonuclease considered to be responsible for the DNA cleavage, but was not suppressed by the protein synthesis inhibitor cycloheximide. ...
https://espace.library.uq.edu.au/view/UQ:403689
北京大学医学部机构知识库([email protected]): Cycloheximide and actinomycin D delay death and affect bcl-2, bax, and ice gene expression in...
An in vitro ischemia model was established and the effect of the metabolic inhibitors cycloheximide (CHX) and actinomycin D (ActD) on apoptosis in astrocytes under ischemia studied. CHX decreased by 75% the number of cells dying after 6 hr of ischemia compared with control cultures. TdT-mediated dUTP nick end labelling (TUNEL) staining of comparable cultures was reduced by 40%. ActD decreased cell death by 60% compared with controls. The number of TUNEL-positive cells was reduced by 38%. The nuclear shrinkage in TUNEL-positive astrocytes in control cultures did not occur in ActD-treated astrocytes, indicating that nuclear shrinkage and DNA fragmentation during apoptosis are two unrelated processes. Expression of bcl-2 (alpha and beta), bax, and Ice in astrocytes under similar ischemic conditions, as measured by quantitative reverse transcription-polymerase chain reaction, indicated that ischemia down-regulated bcl-2 (alpha and beta) and bax. Ice was initially down-regulated from 0 to 4 hr, ...
http://ir.bjmu.edu.cn/handle/400002259/64569
kbrin.a-bldg.louisville.edu
We used the rat visual cortex as a model system to examine the changes in protein synthesis during experience-induced synaptic plasticity. Dark-rearing rats from birth results in a relatively immature visual cortex that maintains the high de- gree of synaptic plasticity characteristic of the critical period (Kirkwood et al., 1995). Exposure of dark-reared rats to light results in a rapid, robust and coordinated burst of experience- driven synaptic plasticity that can be readily monitored at the biochemical and electrophysiological level (Quinlan et al., 1999). In previous work, we showed that visual experience evokes the polyadenylation of ␣-CaMKII mRNA in visual cortex and the elevation of ␣-CaMKII protein in synaptic fractions from this brain region. Moreover, this increase was a direct result of new synthesis because it was sensitive to the translation inhibitor cycloheximide (Wu et al., 1998). Here we show that the experience-induced increase of ␣-CaMKII pro- tein does not require new ...
http://hmamedicalclinic.com/k/kbrin.a-bldg.louisville.edu1.html
A Physical Model Illustrating Protein Synthesis on the Ribosome | The American Biology Teacher | University of California PressA Physical Model Illustrating Protein Synthesis on the Ribosome | The American Biology Teacher | University of California Press
Allen C. Rogerson, Richard W. Cheney Jr.; A Physical Model Illustrating Protein Synthesis on the Ribosome. The American Biology Teacher 1 January 1989; 51 (1): 29-31. doi: https://doi.org/10.2307/4448834. Download citation file:. ...
https://online.ucpress.edu/abt/article/51/1/29/3016/A-Physical-Model-Illustrating-Protein-Synthesis-on
Thymidine (DThyd) | DNA/RNA Synthesis Inhibitor | MedChemExpressThymidine (DThyd) | DNA/RNA Synthesis Inhibitor | MedChemExpress
Thymidine, a pyrimidine deoxynucleoside, is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. - Mechanism of Action & Protocol.
https://www.medchemexpress.com/Thymidine.html
Despite intensive analysis zero therapies targeted contrary to the oncogenic EGFRvIII | Imaging Proteolysis by Living Human...Despite intensive analysis zero therapies targeted contrary to the oncogenic EGFRvIII | Imaging Proteolysis by Living Human...
Despite intensive analysis zero therapies targeted contrary to the oncogenic EGFRvIII can be found in the medical clinic. PTP inhibitors triggered a prominent upsurge in phosphorylation degrees of EGFRvIII on all residues analyzed, including Tyr1045. Oddly enough, hypophosphorylation of the tyrosine continues to be previously reported on several occasions and from the raised stability from the proteins [7, 8]. Certainly, we have not really noticed any degradation from the truncated receptor also in the current presence of cycloheximide to avoid de novo proteins synthesis (Supplementary Amount 2). Statistical evaluation indicated which the change in phosphorylation of specific tyrosines had not been always significant in case there is the wild-type receptor, that will be due to a combined mix of limited awareness of the technique and a comparatively few reproductions (= 3). To check this hypothesis, weve centered on NaOVa, a much less powerful PTP inhibitor of both, and examined its influence on ...
http://healthychoicess.net/despite-intensive-analysis-zero-therapies-targeted-contrary-to-the-oncogenic-egfrviii/
Protein synthesis: steps and processProtein synthesis: steps and process
Protein synthesis or translation is the mechanism by which the triplet base sequence of an mRNA guides the linking of a specific sequence of amino acids to form a polypeptide (protein) or ribosomes. Protein synthesis is the process by which biological cells generate new proteins. ...
https://sciencetechstudy.com/protein-synthesis-steps/
science protein synthesis Flashcards - Cram.comscience protein synthesis Flashcards - Cram.com
Study Flashcards On science protein synthesis at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
http://www.cram.com/flashcards/science-protein-synthesis-317139
Turning over the keys to rural businesses : Community Features : UMN ExtensionTurning over the keys to rural businesses : Community Features : UMN Extension
When a Walmart Supercenter opened in Winona, Minnesota in 2003, there was definitely a churning among local businesses. Eleven years later, what has been the net effect?
http://www.extension.umn.edu/community/news/turning-over-the-keys-to-rural-businesses/
What are the steps involved in protein synthesis when you start with the DNA code in the nucleus and end up with a polypeptide...What are the steps involved in protein synthesis when you start with the DNA code in the nucleus and end up with a polypeptide...
What are the steps involved in protein synthesis when you start with the DNA code in the nucleus and end up with a polypeptide in simple terms please? Find answers now! No. 1 Questions & Answers Place.
https://www.weknowtheanswer.com/q/what-are-the-steps-involved-in-protein-synthesis-when-you-start-with-the-dna-code-in-the-nucleus-and-end-up-with-a-polypeptide-in-simple-terms-please
What Does the Term protein Synthesis Mean? | Reference.comWhat Does the Term protein Synthesis Mean? | Reference.com
The term protein synthesis refers to the making of a protein. This process begins in the nucleus and then continues in the ribosomes. Protein synthesis is comprised of two parts: DNA transcription...
https://www.reference.com/science/term-protein-synthesis-mean-fa7ea0055954d7f6
CiNii Articles - Mycotrienin II, a translation inhibitor that prevents ICAM-1 expression induced by pro-inflammatory...
TASHIRO Etsu , HIRONIWA Naoka , KITAGAWA Mitsuhiro , FUTAMURA Yushi , SUZUKI Shin-ichi , NISHIO Maki , IMOTO Masaya J. Antibiot. (Tokyo) 60(9), 547-553, 2007-09-25 Ichushi Web References (28) Cited by (4) ...
https://ci.nii.ac.jp/naid/10030638820/en/
CODE MORSE-TEXTES EN CLAIRCODE MORSE-TEXTES EN CLAIR
Vous ny trouverez que des informations concernant MA façon de pratiquer le radioamateurisme (tout en respectant les règles en vigueur). Après le QSO etléchange des QSLs, cest une autre façon de continuer
http://f5jbr.free.fr/ameliorer_vitesse_EN.html
CHX10抗体- ChIP Grade|Abcam中国|Anti-CHX10抗体- ChIP Grade(ab16141)CHX10抗体- ChIP Grade|Abcam中国|Anti-CHX10抗体- ChIP Grade(ab16141)
CHX10羊多克隆抗体(ab16141)可与小鼠, 大鼠, 牛, 人样本反应并经WB, IHC, ChIP实验严格验证，被7篇文献引用并得到1个独立的用户反馈。所有产品提供质保服务，中国75%以上现货。
http://www.abcam.cn/chx10-antibody-chip-grade-ab16141.html
Early Intervention Directory - Search Results | Havering DirectoryEarly Intervention Directory - Search Results | Havering Directory
Please do not change the value of the following field. It is used to detect spam and changing its value may result in your submission being ...
https://familyserviceshub.havering.gov.uk/kb5/havering/directory/results.action?sorttype=field&nh=10&practitionerchannel=0&sr=250&sortfield=title&slaction=ADD&itemid=so4sd2xcgg4Wq9YKNAIloKP1g0LpS0Z1LuP13S1K
CycloheximideCycloheximide
... was reported in 1946 by Alma Joslyn Whiffen-Barksdale at the Upjohn Company. Cycloheximide can be used as an ... Cycloheximide has also been used to make isolation of bacteria from environmental samples easier. Cycloheximide has been used ... Cycloheximide is a naturally occurring fungicide produced by the bacterium Streptomyces griseus. Cycloheximide exerts its ... Treating cells with cycloheximide in a time-course experiment followed by western blotting of the cell lysates for the protein ...
https://en.wikipedia.org/wiki/Cycloheximide
Cycloheximide chaseCycloheximide chase
Disadvantages Some disadvantages to conducting cycloheximide chase assays include the toxic nature of cycloheximide. When used ... Cycloheximide chases can be used with a wide variety of model systems and can be implemented to study almost any protein ... For this reason, cycloheximide chases do not typically last for more than 12 hours. This presents a limitation if the turnover ... Cycloheximide is relatively inexpensive compound compared to other drugs and it is effective when used in low doses for short ...
https://en.wikipedia.org/wiki/Cycloheximide_chase
GlutarimideGlutarimide
Sisler, Hugh D.; Siegel, Malcolm R. (1967). "Cycloheximide and Other Glutarimide Antibiotics". Mechanism of Action. pp. 283-307 ... and cycloheximide, a potent inhibitor of protein synthesis. Glutarimide - Sigma-Aldrich Paris, G.; Berlinguet, L.; Gaudry, R.; ...
https://en.wikipedia.org/wiki/Glutarimide
ChlamydosauromycesChlamydosauromyces
It is also cycloheximide resistant. C. punctatus can reproduce both in sexual and asexual forms. The teleomorph phase is ...
https://en.wikipedia.org/wiki/Chlamydosauromyces
Lomentospora prolificansLomentospora prolificans
This species is sensitive to cycloheximide. As this species may be slow to emerge from clinical materials, specimens in which ...
https://en.wikipedia.org/wiki/Lomentospora_prolificans
AcetoxycycloheximideAcetoxycycloheximide
Cycloheximide Mahy, Brian W J (2001). A dictionary of virology (3. ed.). San Diego, Calif. [u.a.]: Academic Press. pp. 2. ISBN ... It can be considered as the acetylated analogue of cycloheximide. It is a potent protein synthesis inhibitor in animal cells ...
https://en.wikipedia.org/wiki/Acetoxycycloheximide
Naganishia adeliensisNaganishia adeliensis
... also grows on 0.01% cycloheximide. Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV, Lumbsch HT, ...
https://en.wikipedia.org/wiki/Naganishia_adeliensis
Streptomyces albulusStreptomyces albulus
... produces acetoxycycloheximide, aciphenol, albanoursin and cycloheximide. List of Streptomyces species LPSN ...
https://en.wikipedia.org/wiki/Streptomyces_albulus
CyanidioschyzonCyanidioschyzon
Yagisawa F; Nishida K; Okano Y; Minoda A; Tanaka K; Kuroiwa T (2004). "Isolation of cycloheximide-resistant mutants of ...
https://en.wikipedia.org/wiki/Cyanidioschyzon
Geomyces pannorumGeomyces pannorum
... is resistant to the antifungal agent cycloheximide. However the growth of this species is inhibited by ...
https://en.wikipedia.org/wiki/Geomyces_pannorum
Eukaryotic ribosomeEukaryotic ribosome
Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin". Nat Chem Biol. 6 (3): 209-217. doi: ... One toxic inhibitor of eukaryotic translation elongation is the glutarimide antibiotic cycloheximide (CHX), which has been co- ...
https://en.wikipedia.org/wiki/Eukaryotic_ribosome
LactimidomycinLactimidomycin
March 2010). "Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin". Nature Chemical Biology. 6 ...
https://en.wikipedia.org/wiki/Lactimidomycin
Translation (biology)Translation (biology)
These include anisomycin, cycloheximide, chloramphenicol, tetracycline, streptomycin, erythromycin, and puromycin. Prokaryotic ...
https://en.wikipedia.org/wiki/Translation_(biology)
BacterivoreBacterivore
Adding cycloheximide can inhibit the growth of bacterivores without affecting some bacterial species, but it has also been ... Tremaine, Sarah C. Mills, Aaron L. (1987). "Inadequacy of the Eucaryote Inhibitor Cycloheximide in Studies of Protozoan Grazing ... "Cycloheximide: Aspects of Inhibition of Protein Synthesis in Mammalian Cells". Science. 146 (3650): 1474-1476. Bibcode:1964Sci ...
https://en.wikipedia.org/wiki/Bacterivore
Alma Joslyn Whiffen-BarksdaleAlma Joslyn Whiffen-Barksdale
List of mycologists Cycloheximide Achlya bisexualis "Alma Whiffen Barksdale (1916-1981)". Smithsonian Institution Archives. ... While there, she discovered the chemical cycloheximide (trade name Actidione), an anti-fungal and anti-bacterial agent produced ... Alma Joslyn Whiffen-Barksdale (October 25, 1916 - July 5, 1981) was an American mycologist who discovered cycloheximide. She ...
https://en.wikipedia.org/wiki/Alma_Joslyn_Whiffen-Barksdale
Mucor racemosusMucor racemosus
It has been shown to adapt to famous antibiotics like cycloheximide, trichodermin and amphotericin B. Cells adapted to ... Shearer G, Jr; Sypherd, PS (March 1988). "Cycloheximide efflux in antibiotic-adapted cells of the fungus Mucor racemosus". ... cycloheximide particularly have been observed to be 40-times more resistant than non-adapted cells to the drug. These adapted ...
https://en.wikipedia.org/wiki/Mucor_racemosus
Ribosome profilingRibosome profiling
This is commonly performed with cycloheximide but other chemicals can be employed. It is also possible to forgo translation ... RNA-ribosome complexes Cycloheximide Nucleases Phenol/Chloroform Reverse transcriptase dNTPs Sequencing method-cDNA library. ... The other elongating regions can be detected by adding antibiotics like cycloheximide that inhibit translocation, ...
https://en.wikipedia.org/wiki/Ribosome_profiling
Harold M. WeintraubHarold M. Weintraub
"Conservative segregation of parental histones during replication in the presence of cycloheximide". Proc Natl Acad Sci USA. 76 ...
https://en.wikipedia.org/wiki/Harold_M._Weintraub
C-5 sterol desaturaseC-5 sterol desaturase
Abe Fumiyoshi; Hiraki Toshiki (2009). "Mechanistic role of ergosterol in membrane rigidity and cycloheximide resistance in ...
https://en.wikipedia.org/wiki/C-5_sterol_desaturase
Microsporum nanumMicrosporum nanum
Like many other dermatophytes, M. nanum is tolerant of the antifungal agent cycloheximide. In addition, M. nanum also exhibits ...
https://en.wikipedia.org/wiki/Microsporum_nanum
ParthenogenesisParthenogenesis
Treatment with cycloheximide, a non-specific protein synthesis inhibitor, enhances parthenote development in swine presumably ... "Effects of Cycloheximide on Parthenogenetic Development of Pig Oocytes Activated by Ultrasound Treatment". Journal of ...
https://en.wikipedia.org/wiki/Parthenogenesis
Adrenal ferredoxinAdrenal ferredoxin
1988). "Human adrenodoxin: cloning of three cDNAs and cycloheximide enhancement in JEG-3 cells". J. Biol. Chem. 263 (7): 3240-4 ...
https://en.wikipedia.org/wiki/Adrenal_ferredoxin
Cln3Cln3
Finally, it was also shown that this delay occurred even with short pulses of cycloheximide, confirming that an unstable ... It was later shown that treatment with the protein synthesis inhibitor cycloheximide delayed Start in yeast, indicating that ... "Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide". Experimental Cell Research ...
https://en.wikipedia.org/wiki/Cln3
Cyclin D1Cyclin D1
Baliga BS, Pronczuk AW, Munro HN (August 1969). "Mechanism of cycloheximide inhibition of protein synthesis in a cell-free ... Obrig TG, Culp WJ, McKeehan WL, Hardesty B (January 1971). "The mechanism by which cycloheximide and related glutarimide ...
https://en.wikipedia.org/wiki/Cyclin_D1
Trichophyton rubrumTrichophyton rubrum
... rubrum cultures can be isolated on both cycloheximide-containing media and cycloheximide-free media. The latter are ... In primary outgrowth on Sabouraud dextrose agar with cycloheximide and antibacterials, contaminating organisms may cause ...
https://en.wikipedia.org/wiki/Trichophyton_rubrum
Trichophyton tonsuransTrichophyton tonsurans
CLS growth is essentially unaffected by antibiotic treatment with chloramphenicol, as well as by cycloheximide. After 5 days of ...
https://en.wikipedia.org/wiki/Trichophyton_tonsurans
Cunninghamella bertholletiaeCunninghamella bertholletiae
Unlike in the case of dimorphic pathogenic fungi, growth of C. bertholletiae is inhibited by cycloheximide. As a member of the ...
https://en.wikipedia.org/wiki/Cunninghamella_bertholletiae
Trichosporon asteroidesTrichosporon asteroides
... grows in a range of media including in the presence of the antifungal agent, cycloheximide. Colonies of ...
https://en.wikipedia.org/wiki/Trichosporon_asteroides
NeothramycinNeothramycin
MC916-C4, a cycloheximide-producing strain, by Umezawa et al. Subsequent testing has shown its capabilities as an anticancer ...
https://en.wikipedia.org/wiki/Neothramycin
AnisomycinAnisomycin
Cycloheximide Anisomycin Archived January 19, 2012, at the Wayback Machine from Sigma Aldrich Grollman, Arthur P. (1967). " ...
https://en.wikipedia.org/wiki/Anisomycin
Roscovitine a particular inhibitor of cyclin dependent kinases and cycloheximide a protein synthesisRoscovitine a particular inhibitor of cyclin dependent kinases and cycloheximide a protein synthesis
Author: Hou, N. N. / Subject: Glycine max / Subject term: cycloheximide - PubAg Search ResultsAuthor: 'Hou, N. N.' / Subject: Glycine max / Subject term: cycloheximide - PubAg Search Results
... cycloheximide Remove constraint Subject term: cycloheximide Start Over ... Glycine max; aluminum; citrates; cultivars; cycloheximide; exudation; mitochondrial membrane; plasma membrane; protein ...
https://pubag.nal.usda.gov/?f%5Bsubject_facet%5D%5B%5D=Glycine+max&f%5Bsubject_term%5D%5B%5D=cycloheximide&per_page=100&q=%22Hou%2C+N.+N.%22&search_field=author&sort=date-asc
IMSEAR at SEARO: Effect of cycloheximide on intestinal transport of methionine & glycine.
Mahmood A. Effect of cycloheximide on intestinal transport of methionine & glycine. Indian Journal of Experimental Biology. ...
https://imsear.searo.who.int/handle/123456789/58239
Sabouraud Dextrose Agar w Chloramphenicol w Cycloheximide - ALPHACHEMSabouraud Dextrose Agar w Chloramphenicol w Cycloheximide - ALPHACHEM
Trụ sở chính: Số 4, đường số 2, KTĐC Sông Phú Định, Phường 16, Quận 8, Thành phố Hồ Chí ...
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Cycloheximide (100 mg/ml, DMSO) - 5x1ml - LabCloud Plus+
Cycloheximide (100 mg/ml) - Method: Prepared in DMSO. - Storage: -20° C ... Cycloheximide (100 mg/ml, DMSO) - 5x1ml. * $12000 $120.00 Unit price/ per ... Components: Cycloheximide (100 mg/ml) - Method: Prepared in DMSO. - Storage: -20° C ...
https://plus.labcloudinc.com/products/abt-245-5x1ml
Legionnaires Disease: Laboratory Guidance for Processing Samples | CDCLegionnaires Disease: Laboratory Guidance for Processing Samples | CDC
The first, designated PCV, contains polymyxin B, cycloheximide, and vancomycin. The second, GPCV, is identical to PCV except ...
https://www.cdc.gov/legionella/labs/procedures-manual.html
Results for Diabetes | Abcam: antibodies, proteins, kits...Results for 'Diabetes' | Abcam: antibodies, proteins, kits...
Cycloheximide, Protein synthesis inhibitor (ab120093) Specific References (22) Description:. Protein synthesis inhibitor ...
https://www.abcam.com/products?selected.researchAreas=Biochemicals--Research+Area--Diabetes
Arsenic activates EGFR pathway signaling in the lungArsenic activates EGFR pathway signaling in the lung
Cycloheximide / pharmacology * EGF Family of Proteins * Epidermal Growth Factor / metabolism * Epithelial Cells / metabolism ...
https://pubmed.ncbi.nlm.nih.gov/19168569/
Cycloheximide and disulfoton are positive in the photoclastogencity assay but do not absorb UV irradiation: another example of...Cycloheximide and disulfoton are positive in the photoclastogencity assay but do not absorb UV irradiation: another example of...
Cycloheximide and disulfoton are positive in the photoclastogencity assay but do not absorb UV irradiation: another example of ... Follow-up testing showed that neither cycloheximide nor disulfoton was positive in the 3T3-NRU assay, the standard in vitro ... The results showed that cycloheximide, disulfoton and tetracycline were clastogenic in CHO cells following UVR exposure (solar- ... These data suggest that both cycloheximide and disulfoton are pseudophotoclastogens, like zinc oxide. Together, these data ...
https://pesquisa.bvsalud.org/saudepublica/resource/pt/mdl-18227081
PP3150 - Dermatophyte Test Medium (DTM) with Chloramphenicol & Cyclohexamide (Actidione) - E & O Laboratories Ltd
PP3150 - Dermatophyte Test Medium (DTM) with Chloramphenicol & Cyclohexamide (Actidione). Home/Pre Poured Petri Dishes/Clinical ... Cyclohexamide (Actidione) is also added to suppress the growth of most yeasts and saprophytic fungi. Dermatophytes appear as ... Dermatophyte Test Medium with Chloramphenicol & Cyclohexamide (Actidione). This is a selective medium for the isolation of ... Send Enquiry for PP3150 - Dermatophyte Test Medium (DTM) with Chloramphenicol & Cyclohexamide (Actidione) ...
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Pharmacological induction of Hsp70 protects apoptosis-prone cells from doxorubicin: comparison with caspase-inhibitor- and...Pharmacological induction of Hsp70 protects apoptosis-prone cells from doxorubicin: comparison with caspase-inhibitor- and...
... cycloheximide 10 μg/ml); ActD: 2 μg/ml actinomycin D; CAM: 500 nM camptothecin; FL: 500 nM flavopiridol. MTT assay was ... cycloheximide (CHX), etoposide and 4,5 dimethyl-2-yl 2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma Chemical ...
https://www.nature.com/articles/4401812?error=cookies_not_supported&code=00e8cd1f-a723-4840-a08b-7cd678fbd046
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...
C,D) In the absence and presence of cycloheximide (CHX), whole cells lysates were prepared and immunoblotting was performed by ... We then examined the effects of coffee extract on LPS-induced IκBα degradation in the absence and presence of cycloheximide ( ...
https://www.nature.com/articles/s41598-020-59380-x?error=cookies_not_supported&code=970d1222-7b9c-4f10-a5de-fa36a01afc50
Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening...
Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening ... Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening ... Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening ... 53Cycloheximide is a drug that inhibits protein synthesis by cells. Predict what effect cyclohximide would have on the greening ...
https://qualityassignments.net/2022/03/24/cycloheximide-is-a-drug-that-inhibits-protein-synthesis-by-cells-predict-what-effect-cyclohximide-would-have-on-the-greening-response/
CYCLOHEXIMIDE MODIFICATION OF THE EFFECTS OF INSULIN ON CALCIUM TRANSPORT IN CARDIAC SARCOLEMMA - статья | ИСТИНА -...CYCLOHEXIMIDE MODIFICATION OF THE EFFECTS OF INSULIN ON CALCIUM TRANSPORT IN CARDIAC SARCOLEMMA - статья | ИСТИНА -...
CYCLOHEXIMIDE MODIFICATION OF THE EFFECTS OF INSULIN ON CALCIUM TRANSPORT IN CARDIAC SARCOLEMMAстатья ... Possible mechanisms of modifying effect of cycloheximide on the function of insulin-dependent regulatory system in the ... Pretreatment of myocardial preparation with cycloheximide in low concentrations completely blocks the inhibitory insulin effect ...
https://istina.ips.ac.ru/publications/article/79298299/
British Library EThOS: Investigating temperature signalling pathways in Arabidopsis thaliana using small moleculesBritish Library EThOS: Investigating temperature signalling pathways in Arabidopsis thaliana using small molecules
In addition, the translation inhibitor cycloheximide was identified as a repressor of heat-inducible genes and an activator of ... heat stress ; HSP70 ; chemical genomics ; Syngenta ; cold shock ; calcium signalling ; translation inhibitor ; cycloheximide ; ... or cycloheximide-induced gene expression, thus implicating translational machinery in the regulation of temperature signalling ...
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.763754
Advanced Search Results - Public Health Image Library(PHIL)Advanced Search Results - Public Health Image Library(PHIL)
Categories: Cycloheximide Image Types: Photo, Illustrations, Video, Color, Black&White, PublicDomain, CopyrightRestricted 4 ...
https://phil.cdc.gov/AdvancedSearchResults.aspx?Search=Cycloheximide&parentid=20321&catid=7469
Onychomycosis: Practice Essentials, Background, PathophysiologyOnychomycosis: Practice Essentials, Background, Pathophysiology
Medium without cycloheximide (Sabouraud glucose agar, Littman oxgall medium, or inhibitory mold agar) isolates yeasts and ... Medium with cycloheximide (dermatophyte test medium [DTM], Mycosel, or Mycobiotic) selects for dermatophytes ...
https://www.medscape.com/answers/1105828-22687/how-is-a-poor-response-to-treatment-demonstrated-in-onychomycosis
Axonal Protein Synthesis and Degradation Are Necessary for Efficient Growth Cone Regeneration | Journal of NeuroscienceAxonal Protein Synthesis and Degradation Are Necessary for Efficient Growth Cone Regeneration | Journal of Neuroscience
... cycloheximide, 91 ± 6%; lactacystin, 89 ± 5%) and retinal (cycloheximide, 88 ± 7%; lactacystin, 90 ± 4%) cultures, indicating ... a, Time-lapse images showing the typical responses of axons to axotomy and the effects of cycloheximide and lactacystin. Axons ... Effects of protein synthesis inhibitor cycloheximide (25 μm) and proteasome-dependent degradative inhibitor lactacystin (10 μm ... Cultures were treated with cycloheximide and lactacystin, and axonal elongation was monitored. The proportion of axons whose ...
https://www.jneurosci.org/content/25/2/331?ijkey=3b90f96fa750574132e699e49f4456d45a6f8b11&keytype2=tf_ipsecsha
Pellino 1 Communicates Intercellular Signaling in Chronic Skin Inflammatory Microenvironment | bioRxivPellino 1 Communicates Intercellular Signaling in Chronic Skin Inflammatory Microenvironment | bioRxiv
G. Representative cycloheximide chase assay of IFR4 turnover in HaCaT cells transfected with control vector (pMyc) or a vector ... MG132 and cycloheximide, A.G Scientific. All antibodies used in this study are listed in Appendix Table S1. ... Whole cell lysates were harvested at various time points after exposure to cycloheximide and IRF4 levels were determined by ... we transfected HaCaT cells with plasmid encoding Myc-Peli1 in the presence of protein synthesis inhibitor cycloheximide (CHX) ( ...
https://www.biorxiv.org/content/10.1101/334433v1.full
JCI - Production of Antibodies Specific for Fc, Fab′, and Streptokinase-Streptodornase In Vitro by Peripheral Blood Cells from...JCI - Production of Antibodies Specific for Fc, Fab′, and Streptokinase-Streptodornase In Vitro by Peripheral Blood Cells from...
This early release of Ab was blocked by cycloheximide. With or without PWM, PBL from normal donors did not release IgM anti-Fc ...
https://www.jci.org/articles/view/110443/scanned-page/83
Recommendations for the Prevention and Management of Chlamydia trachomatis Infections, 1993Recommendations for the Prevention and Management of Chlamydia trachomatis Infections, 1993
Cycloheximide-treated McCoy cells are commonly used to culture chlamydia. Different McCoy cell lines exist and some have ...
https://wonder.cdc.gov/wonder/prevguid/p0000222/p0000222.asp
Phosphorylated ATF1 at Thr184 promotes metastasis and regulates MMP2 expression in gastric cancer | Journal of Translational...Phosphorylated ATF1 at Thr184 promotes metastasis and regulates MMP2 expression in gastric cancer | Journal of Translational...
... luciferase reporter assay and cycloheximide experiment. The Cancer Genome Atlas (TCGA) data were used to analyze the expression ... Cycloheximide treatment. 50 μg/ml cycloheximide was applied to the MGC803 cells transfected with Vector, ATF1-WT, ATF1-T184D ... D Cycloheximide experiment showed effect of p-ATF1-T184 on the stability of ATF1 protein in MGC803 ... luciferase reporter assay and cycloheximide experiment. The Cancer Genome Atlas (TCGA) data were used to analyze the expression ...
https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-022-03361-3
The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane | eLifeThe AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane | eLife
Louis, MO)) and treated with 3-indoleacetic acid (auxin, 500 μM) (Sigma), cycloheximide (100 μg/mL) (Sigma) or DMSO vehicle as ...
https://elifesciences.org/articles/28507
Oxoid - Product DetailOxoid - Product Detail
STAA Selective Supplement SR0151 contains cycloheximide and thallous acatate and is toxic if swallowed, inhaled or if in ... STAA Medium is made selective by the inclusion of streptomycin sulphate, thallous acetate and actidione (cycloheximide). STA ... The incorporation of cycloheximide serves to further inhibit yeasts and filamentous fungi. ... Medium is modified from the original formulation by removal of the toxic selective agent cycloheximide as recommended by the ...
http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=SR0151&cat=&c=UK&lang=EN
Cooling shock induction of protein synthesis by larvae of Galleria mellonella (L.)(Insecta: Lepidoptera) | Masarykova univerzitaCooling shock induction of protein synthesis by larvae of Galleria mellonella (L.)(Insecta: Lepidoptera) | Masarykova univerzita
Galleria mellonella; cooling shock; electrophoresis; actinomycin D; cyclohexamide; haemolymph proteins; osmotic pressure; ...
https://www.muni.cz/vyzkum/publikace/384971
NIOSHTIC-2 Search Results - Basic View
Regulation of TCDD-induced degradation of Ah receptor by cycloheximide. Authors. Ma Q; Baldwin KT; Renzelli AJ; Antonini JM ...
http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=asthma+OR+%27allerg%2A%27+OR+%27immune%2A%27&f1=%2A&Startyear=&Adv=0&terms=1&EndYear=&Limit=10000&sort=&D1=10&PageNo=2993&RecNo=2993&View=b&
Cellulose synthase interactive1- and microtubule-dependent cell wall architecture is required for acid growth in Arabidopsis...Cellulose synthase interactive1- and microtubule-dependent cell wall architecture is required for acid growth in Arabidopsis...
Cycloheximide inhibits growth and causes the tissues to remove protons from the medium. Neutral buffers (pH 7.0) inhibit auxin- ...
https://www.osti.gov/pages/biblio/1631346-cellulose-synthase-interactive1-microtubule-dependent-cell-wall-architecture-required-acid-growth-arabidopsis-hypocotyls
ChloramphenicolActidioneActinomycinTranslation inhibitorProtein synthesisGlycinePolymyxinAssaySelectiveReceptorCellsGeneRegulationDrugsMediumEffectCultureSuggestMethodResistance to cycloheximideInhibitionInhibitor of protein synthesisTranslation elongationMcCoyProteinsInhibitsAntifungalChloramphenicolNeuronsSabouraudStabilizationAntibioticCellsBacterialStimulationAccumulationGeneSupplementInfectionTreatmentCandidaEffectsInjectionPresenceResponsesMediumAdministrationPlasmaMethodTime
Chloramphenicol2
  • We isolated P. orbiculare on a peptone-glucose-yeast extract medium containing chloramphenicol and cycloheximide, and overlaid with olive oil. (who.int)
  • Culturing is performed routinely with Sabouraud agar and the addition of cycloheximide and chloramphenicol. (medscape.com)
Actidione2
  • Cyclohexamide (Actidione) is also added to suppress the growth of most yeasts and saprophytic fungi. (eolabs.com)
  • STAA Medium is made selective by the inclusion of streptomycin sulphate, thallous acetate and actidione (cycloheximide). (oxoid.com)
Actinomycin2
  • The selection of apoptosis inducers include actinomycin D , camptothecin , cycloheximide , dexamethasone , doxorubicin and etoposide . (gbiosciences.com)
  • Elevations of δ-aminolaevulic acid synthetase activity caused by dehydroepiandrosterone could be abolished by the antibiotics cycloheximide and actinomycin D. (portlandpress.com)
Translation inhibitor2
  • In addition, the translation inhibitor cycloheximide was identified as a repressor of heat-inducible genes and an activator of early cold-inducible genes. (bl.uk)
  • By using a proteasome inhibitor MG132 and a translation inhibitor cyclohexamide, we showed that CDCA reduced HIF-1α protein by decreasing its translation but not by enhancing its degradation. (elsevier.com)
Protein synthesis1
  • Application of protein synthesis inhibitors (cycloheximide and anisomycin) to cut axons, including axons whose cell bodies were removed, or proteasome inhibitors (lactacystin and N -acetyl-Nor-Leu-Leu-Al) all result in a reduction in the proportion of transected axons able to reform growth cones. (jneurosci.org)
Glycine1
  • IMSEAR at SEARO: Effect of cycloheximide on intestinal transport of methionine & glycine. (who.int)
Polymyxin1
  • from clinical samples by using buffered charcoal yeast extract (BCYE) medium, with and without antimicrobial drugs, including polymyxin B, cycloheximide, and vancomycin. (cdc.gov)
Assay4
  • Cycloheximide and disulfoton are positive in the photoclastogencity assay but do not absorb UV irradiation: another example of pseudophotoclastogenicity? (bvsalud.org)
  • To explore this further, four coded compounds ( aminotriazole , propantheline bromide , cycloheximide and disulfoton ) were evaluated for their potential response in a photoclastogenicity assay in cultured Chinese hamster ovary (CHO) cells . (bvsalud.org)
  • Follow-up testing showed that neither cycloheximide nor disulfoton was positive in the 3T3-NRU assay, the standard in vitro regulatory test for phototoxicity , a result consistent with their inability to absorb UVR. (bvsalud.org)
  • The regulation of MMP2 by p-ATF1-T184 was investigated by a series of experiments including quantitative RT-PCR, western blot, gelatin zymography assay, Chromatin immunoprecipitation (ChIP), luciferase reporter assay and cycloheximide experiment. (biomedcentral.com)
Selective2
  • STA Medium is modified from the original formulation by removal of the toxic selective agent cycloheximide as recommended by the NMKL. (oxoid.com)
  • STAA Selective Supplement SR0151 contains cycloheximide and thallous acatate and is toxic if swallowed, inhaled or if in contact with skin. (oxoid.com)
Receptor3
  • Regulation of TCDD-induced degradation of Ah receptor by cycloheximide. (cdc.gov)
  • The changes in Fc receptor affinity and maximum binding of the PMN were reversible in short-term culture, an effect which was prevented by cycloheximide. (elsevier.com)
  • cycloheximide prevented enhancement of phagocytosis, Fc receptor capacity, and colony formation. (aai.org)
Cells4
  • The results showed that cycloheximide , disulfoton and tetracycline were clastogenic in CHO cells following UVR exposure (solar-simulated light at 700 mJ/cm(2)) but not in the absence of UVR. (bvsalud.org)
  • Cycloheximide is a drug that inhibits protein synthesis by cells. (qualityassignments.net)
  • https://qualityassignments.net/wp-content/uploads/2020/07/QAlogo3.png 0 0 Paul https://qualityassignments.net/wp-content/uploads/2020/07/QAlogo3.png Paul 2022-03-24 04:58:53 2022-03-24 04:58:53 Cycloheximide is a drug that inhibits protein synthesis by cells. (qualityassignments.net)
  • Culture requires growth in cycloheximide-treated McCoy or HeLa cells, and even under these conditions, yields of only 30-50% are reported. (medscape.com)
Gene1
  • Furthermore, it was demonstrated that cold shock causes rapid translation repression in A. thaliana seedlings and that the elongation factor LOS1 is involved in cold- or cycloheximide-induced gene expression, thus implicating translational machinery in the regulation of temperature signalling in plants. (bl.uk)
Regulation1
  • Moreover, treatment with cycloheximide inhibited the compensatory regulation. (tubitak.gov.tr)
Drugs1
  • The scientists built upon previous research, which tested microfluidic screening processes for chemicals, such as potassium cyanide, cycloheximide and chemotherapy drugs. (newswise.com)
Medium1
  • cycloheximide 0.5 g/L were added to the Also, Sharquie, Al-Rubyae and Al-Rawi medium. (who.int)
Effect2
  • Pretreatment of myocardial preparation with cycloheximide in low concentrations completely blocks the inhibitory insulin effect on Ca2+-current due, probably, to a decrease in peptide formation. (ac.ru)
  • Possible mechanisms of modifying effect of cycloheximide on the function of insulin-dependent regulatory system in the myocardium, are discussed. (ac.ru)
Culture1
  • Diagnosis is made by microscopic examination followed by fungal culture using cycloheximide-free media. (logicalimages.com)
Suggest1
  • These data suggest that both cycloheximide and disulfoton are pseudophotoclastogens, like zinc oxide . (bvsalud.org)
Method1
  • Components: Cycloheximide (100 mg/ml) - Method: Prepared in DMSO. (labcloudinc.com)
Resistance to cycloheximide1
Inhibition5
  • This suggests that rapidly transported DBH, depleted from the nerve by cycloheximide‐induced inhibition of protein synthesis, is more highly associated with intraneuronal particles than is slowly transported or stationary DBH. (elsevier.com)
  • 15. Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme. (nih.gov)
  • Conversely, cycloheximide significantly (P=0.0001) inhibited biofilm formation in all C. albicans strains(126) and its effect was even 3 fold more pronounced than EDTA inhibition, probably due to its attenuation of proteins (enzymes) and/or complex molecules necessary for biofilm formation. (bioinformation.net)
  • Cycloheximide inhibition decreased NH₄ ⁺ -¹⁵N enrichment and reduced N₂O fluxes while reducing the contribution of codenitrification to total N₂O fluxes by ≥ 66 and ≥ 42%, respectively. (lincoln.ac.nz)
  • Model Structure * * ABSTRACT: Inhibition of protein synthesis by cycloheximide blocks * subsequent division of a mammalian cell, but only if the cell * is exposed to the drug before the restriction point (i.e. within * the first several hours after birth). (nih.gov)
Inhibitor of protein synthesis1
  • When the HepG2 cells were treated with cycloheximide, a general inhibitor of protein synthesis, the loss of CYP3A4 protein was accelerated by cotreatment with either proteasome or NF-kappaB inhibitors. (nih.gov)
Translation elongation2
  • They tested two translation inhibitors -- cycloheximide (inhibitor of translation elongation) and emetine (inhibits 40S ribosomal protein S14) for their ability to inhibit SARS-CoV-2 replication. (scienceboard.net)
  • The third Ribo-seq library was prepared from cells treated with the translation elongation inhibitor cycloheximide (CHX), and gives a snap-shot of actively translating ribosomes across the body of the translated ORF. (ucsc.edu)
McCoy3
  • A through I. In a study of 194 cervical and urethral specimens from women, cycloheximide treatment of McCoy cells was found to be more efficient than 5-iodo-2-deoxyuridine treatment for the isolation of C. trachomatis. (nih.gov)
  • We have compared the two monoclonal antibodies with our routine isolation method, which utilises Giemsa staining of cycloheximide treated McCoy cell cultures. (bmj.com)
  • It can be grown on any of these cell lines treated with cycloheximide: McCoy, HEp-2, HeLa, or buffalo green monkey cells. (medscape.com)
Proteins1
  • Cycloheximide inhibited the accumulation of ubiquitinylated proteins and hampered A0-induced cell death process. (gla.ac.uk)
Inhibits2
  • Administration of cycloheximide inhibits the rise in N-acetyltransferase and N-acetylserotonin, but not melatonin. (nih.gov)
  • Cycloheximide, 50ml (1mg/ml) Solution inhibits brewers yeast growth and is used as an ingredient in media plate preparation. (brewculture.com)
Antifungal2
  • The aim of this study was to determine in vitro effects of EDTA, cycloheximide, and heparin-benzyl alcohol preservative on C. albicans (126) and non-albicans (31)vaginal yeast isolates biofilm formations and their susceptibility against three antifungal Etest strips. (bioinformation.net)
  • To improve the ability of the yeast to remove the metals, it was mutated by Nitrosoguanidine, and among different mutants, it was found that three of them designated as Sc6-1, Sc6-2 and Sc6-3, were more resistant to the antifungal cycloheximide in a concentration of 5 and 10 µg/ ml. (edu.iq)
Chloramphenicol1
  • We isolated P. orbiculare on a peptone-glucose-yeast extract medium containing chloramphenicol and cycloheximide, and overlaid with olive oil. (who.int)
Neurons1
  • When neurons are treated with cycloheximide to examine for newly synthesized Nrf-2, we found that the Nrf-2 stability is largely decreased. (nih.gov)
Sabouraud1
  • Sur 35 échantillons de boue cultivés sur de la gélose de Sabouraud avec du cycloheximide et du chloramphénicol, 326 colonies fongiques appartenant à sept espèces ont été isolées. (who.int)
Stabilization2
  • Superinducibility by cycloheximide is due to two effects: prolonged transcription and stabilization of mRNAs. (nih.gov)
  • Translation ribosome profiling (TRP) recognizes RNA fragments covered with the ribosome equipment after stabilization with cycloheximide (18). (stopvivisection.info)
Antibiotic1
  • The glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. (nih.gov)
Cells6
  • Cycloheximide was used at concentrations that depressed, but did not completely inhibit, the metabolism of the eucaryotic host cells. (nih.gov)
  • In studies on different immunotypes of C. trachomatis cultured in the yolk sac of embryonated hen eggs, the cycloheximide technique was compared with a method using pretreatment of cells with 5-iodo-2-deoxyuridine. (nih.gov)
  • Among such cells, a subpopulation responsive to the bitter taste stimuli quinine and cycloheximide, and aversive concentrations of sodium, cofired to agonists of nocisensitive TRP channels, including capsaicin, mustard oil, and noxious heat. (jneurosci.org)
  • Multiple green fluorescent punctae representing the US27 protein were present in WT- and US27synthesis, we treated cells with cycloheximide overnight to block protein synthesis prior to computer virus contamination. (casein-kinases.com)
  • If cycloheximide is later removed from the culture medium, treated * cells will return to the division cycle, showing a complex pattern * of division times post-treatment, as first measured by Zetterberg * and colleagues. (nih.gov)
  • HeLa cells were infected with (A-B) or serovar E (C-D) and exposed to the DAMPs cAMP (1 mM), ATP (1 mM), or ADO (50 M, plus 25 M EHNA) in incubation medium, in the presence (A, C) or absence (B, D) of 1 1 g/ml cycloheximide, immediately after infection. (bioednet.org)
Bacterial1
  • An overview of Genetic Toxicology Bacterial Mutagenicity study conclusions related to Cycloheximide (66-81-9). (nih.gov)
Stimulation1
  • Cycloheximide attenuated Ang-(1-7) stimulation of NET expression suggesting that Ang-(1-7) stimulates NET synthesis. (conicet.gov.ar)
Accumulation2
  • 4. A single local injection of cycloheximide (10 micrograms/site) did not inhibit neutrophil accumulation or oedema formation produced by 100% Arthus anti-sera. (ox.ac.uk)
  • Cycloheximide (10 micrograms/site) did not significantly inhibit the neutrophil accumulation induced by LPS (0.1 microgram/site). (ox.ac.uk)
Gene2
  • 2001) Synthesis of several light-harvesting complex I polypeptides is blocked by cycloheximide in symbiotic chloroplasts in the sea slug, Elysia chlorotica (Gould): A case for horizontal gene transfer between alga and animal? (seaslugforum.net)
  • This increase in FQR1 mRNA abundance is not diminished by the protein synthesis inhibitor cycloheximide, demonstrating thatFQR1 is a primary auxin-response gene. (ipb-halle.de)
Supplement1
  • Resumen en inglés ABSTRACT Influence of long-term palm and corn oil diet supplement administration on rabbit aortic rings vasoconstriction and vasodilatation. (worldwidescience.org)
Infection1
  • They were produced at early (3h) and immediate-early (2h) times post-infection respectively as determined by cycloheximide and phosphonoacetic acid treatment but were continuously expressed up to at least 24h post-infection. (bl.uk)
Treatment3
  • Unexpectedly, 5-methoxytryptophol increases after cycloheximide treatment. (nih.gov)
  • Cycloheximide treatment did not block the ICI effect. (nih.gov)
  • Recovery of enzymatic activity in vivo, which occurred within 24 h of low-dose MDMA treatment, appeared not to involve synthesis of new enzyme protein, because the return of enzymatic activity was not prevented by prior cycloheximide. (erowid.org)
Candida1
  • El Candida score resultó de 3 en el 77.7% de los casos. (bvsalud.org)
Effects4
  • Effects of cycloheximide and aminophylline on 5-methoxytryptophol and melatonin contents in the chick pineal gland. (nih.gov)
  • These effects were inhibited by cycloheximide. (gla.ac.uk)
  • Resumen en inglés Purpose: To study the effects of mitomycin-C (MMC) on the corneal endothelium after pterygium surgery. (worldwidescience.org)
  • 1. In this study, the effects of a protein synthesis inhibitor, cycloheximide, and a soluble tumour necrosis factor (TNF) binding/IgG fusion protein, p55-sf2, on the priming and challenge stages of the local Shwartzman reaction (LSR) were assessed and compared with their effects on the acute inflammatory response induced by recombinant human tumour necrosis factor-alpha (rhTNF), lipopolysaccharide (LPS) and a reversed passive Arthus (RPA) reaction in rabbit skin. (ox.ac.uk)
Injection1
  • In the LSR, priming i.d. injections of LPS caused a dose-dependent increase in haemorrhage and plasma leakage at skin sites after challenge with LPS (two injections of 100 micrograms, i.v.). Co-injection of a single dose of cycloheximide (10 micrograms/site) with LPS (30 micrograms/site) caused a marked reduction in the amount of haemorrhage. (ox.ac.uk)
Presence4
  • This decrease in HDAC2 protein expression was reversed by curcumin even in the presence of cycloheximide, a protein synthesis inhibitor. (nih.gov)
  • All 10 RNAs were superinduced in the presence of cycloheximide. (nih.gov)
  • The presence of keratinophilic fungi occur abundantly in the keratinophilic fungi was confirmed by sludge environment and the influence of en lowpower microscopic examination. (who.int)
  • presence of the paternal pronucleus assists embryo in overcoming cycloheximide induced abnormalities in zygotic mitosis. (cameras2019.info)
Responses1
  • Local cycloheximide (10 micrograms/site) administered immediately before LSR challenge did not affect the responses produced in the LSR. (ox.ac.uk)
Medium2
  • Tissue culture medium and serum were from Invitrogen Life Technologies (Carlsbad, CA). Forskolin, cycloheximide, indomethacin, phorbol 12-myristate 13-acetate (PMA), 5-bromo-2'-deoxyuridine (BrdU), protease inhibitor mixture (P-2714), 3-isobutyl-1-methylxanthine (IBMX), and phosphatase inhibitor cocktail I were from Sigma (St. Louis, MO). (medscape.com)
  • cycloheximide 0.5 g/L were added to the Also, Sharquie, Al-Rubyae and Al-Rawi medium. (who.int)
Administration1
  • Administration of cycloheximide, 10 mg/kg s.c. led within 4 h to an approx 30% reduction of dopamine‐β‐hydroxylase (DBH) activity in the abdominal portion of rat sciatic nerves. (elsevier.com)
Plasma1
  • Resumen en inglés Using endothelium-denuded rat aortic rings incubated in Ca2+-free solution, we assessed the ability of testosterone to influence the contractile effect of phenylephrine, and the increase in resting tone (IRT) associated with Ca2+ ability to cross the plasma membrane. (worldwidescience.org)
Method1
  • The cycloheximide method gave greater numbers of inclusion-forming units per cover slip for all the immunotypes of trachoma-inclusion conjunctivitis agents tested, i.e. (nih.gov)
Time2
  • Over a 3-h time course, nevertheless, alpha-thrombin-induced secretion of PDGFc was not prevented by cycloheximide. (aidstar-one.com)
  • parental or Wnt3a+) treated with 25?g/ml cycloheximide (CHX) or dimethyl sulfoxide (DMSO) for the time indicated (0C24?h). b Assessment of complementary DNA (cDNA) sequence in control liver (CTRL) and TKO HCC. (yearofevolution.org)
Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories
Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories (cdc.gov)
Legionnaires Disease: Laboratory Guidance for Processing Samples | CDC
Legionnaires Disease: Laboratory Guidance for Processing Samples | CDC (cdc.gov)
Results for 'Diabetes' | Abcam: antibodies, proteins, kits...
Results for 'Diabetes' | Abcam: antibodies, proteins, kits... (abcam.com)
Discovering and Differentiating New and Emerging Clonal Populations of Chlamydia trachomatis with a Novel Shotgun Cell Culture...
Discovering and Differentiating New and Emerging Clonal Populations of Chlamydia trachomatis with a Novel Shotgun Cell Culture... (wwwnc.cdc.gov)
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |...
Pyrocatechol, a component of coffee, suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2 |... (nature.com)
Onychomycosis: Practice Essentials, Background, Pathophysiology
Onychomycosis: Practice Essentials, Background, Pathophysiology (medscape.com)
Tjp1 (tight junction protein 1) - Rat Genome Database
Tjp1 (tight junction protein 1) - Rat Genome Database (rgd.mcw.edu)
Cooling shock induction of protein synthesis by larvae of Galleria mellonella (L.)(Insecta: Lepidoptera) | Masarykova univerzita
Cooling shock induction of protein synthesis by larvae of Galleria mellonella (L.)(Insecta: Lepidoptera) | Masarykova univerzita (muni.cz)
Pellino 1 Communicates Intercellular Signaling in Chronic Skin Inflammatory Microenvironment | bioRxiv
Pellino 1 Communicates Intercellular Signaling in Chronic Skin Inflammatory Microenvironment | bioRxiv (biorxiv.org)
JCI - Production of Antibodies Specific for Fc, Fab′, and Streptokinase-Streptodornase In Vitro by Peripheral Blood Cells from...
JCI - Production of Antibodies Specific for Fc, Fab′, and Streptokinase-Streptodornase In Vitro by Peripheral Blood Cells from... (jci.org)
RCP induces FAK phosphorylation and ovarian cancer cell invasion with inhibition by curcumin | Experimental & Molecular Medicine
RCP induces FAK phosphorylation and ovarian cancer cell invasion with inhibition by curcumin | Experimental & Molecular Medicine (nature.com)
Phosphorylated ATF1 at Thr184 promotes metastasis and regulates MMP2 expression in gastric cancer | Journal of Translational...
Phosphorylated ATF1 at Thr184 promotes metastasis and regulates MMP2 expression in gastric cancer | Journal of Translational... (translational-medicine.biomedcentral.com)
The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane | eLife
The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane | eLife (elifesciences.org)
Antibiotics | SCBT - Santa Cruz Biotechnology
Antibiotics | SCBT - Santa Cruz Biotechnology (scbt.com)
Mycotic Brain Abscess Caused by Opportunistic Reptile Pathogen - Volume 11, Number 2-February 2005 - Emerging Infectious...
Mycotic Brain Abscess Caused by Opportunistic Reptile Pathogen - Volume 11, Number 2-February 2005 - Emerging Infectious... (wwwnc.cdc.gov)
Marizomib sensitizes primary glioma cells to apoptosis induced by a latest-generation TRAIL receptor agonist | Cell Death &...
Marizomib sensitizes primary glioma cells to apoptosis induced by a latest-generation TRAIL receptor agonist | Cell Death &... (nature.com)
(researchgate.net)
Hyperacidification of Vacuoles by the Combined Action of Two Different P-ATPases in the Tonoplast Determines Flower Color: Cell...
Hyperacidification of Vacuoles by the Combined Action of Two Different P-ATPases in the Tonoplast Determines Flower Color: Cell... (cell.com)
Mice Create What-Where-When Hippocampus-Dependent Memories of Unique Experiences | Journal of Neuroscience
Mice Create What-Where-When Hippocampus-Dependent Memories of Unique Experiences | Journal of Neuroscience (jneurosci.org)
Toll-like receptor 4 mediates cross-talk between peroxisome proliferator-activated receptor γ and nuclear factor-κB in...
Toll-like receptor 4 mediates cross-talk between peroxisome proliferator-activated receptor γ and nuclear factor-κB in... (mayoclinic.pure.elsevier.com)
Compensatory expression regulation of highly homologous proteins HNRNP by YAN CHANG, XIAO FENG LU et al.
"Compensatory expression regulation of highly homologous proteins HNRNP" by YAN CHANG, XIAO FENG LU et al. (journals.tubitak.gov.tr)
Proposition 65 List of Elabscience Chemicals
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Histone methyltransferase DOT1L coordinates AR and MYC stability in prostate cancer | Nature Communications
Histone methyltransferase DOT1L coordinates AR and MYC stability in prostate cancer | Nature Communications (nature.com)
Ophthalmologic Manifestations of Escherichia Coli Workup: Laboratory Studies, Imaging Studies, Procedures
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Trusted Partner for Biochemicals and Antibiotics - AG Scientific
Trusted Partner for Biochemicals and Antibiotics - AG Scientific (agscientific.com)