Cystathionine beta-Synthase: A multifunctional pyridoxal phosphate enzyme. In the second stage of cysteine biosynthesis it catalyzes the reaction of homocysteine with serine to form cystathionine with the elimination of water. Deficiency of this enzyme leads to HYPERHOMOCYSTEINEMIA and HOMOCYSTINURIA. EC 4.2.1.22.Protein Synthesis Inhibitors: Compounds which inhibit the synthesis of proteins. They are usually ANTI-BACTERIAL AGENTS or toxins. Mechanism of the action of inhibition includes the interruption of peptide-chain elongation, the blocking the A site of ribosomes, the misreading of the genetic code or the prevention of the attachment of oligosaccharide side chains to glycoproteins.Dactinomycin: A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)Puromycin: A cinnamamido ADENOSINE found in STREPTOMYCES alboniger. It inhibits protein synthesis by binding to RNA. It is an antineoplastic and antitrypanosomal agent and is used in research as an inhibitor of protein synthesis.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.Social Validity, Research: Evaluation of the degree of acceptance for the immediate variables associated with a procedure or program designed to change behavior. This includes the social significance of the goals of treatment, the social appropriateness of the treatment procedures, and the social importance of the effects of treatments.Cellulitis: An acute, diffuse, and suppurative inflammation of loose connective tissue, particularly the deep subcutaneous tissues, and sometimes muscle, which is most commonly seen as a result of infection of a wound, ulcer, or other skin lesions.Emetine: The principal alkaloid of ipecac, from the ground roots of Uragoga (or Cephaelis) ipecacuanha or U. acuminata, of the Rubiaceae. It is used as an amebicide in many different preparations and may cause serious cardiac, hepatic, or renal damage and violent diarrhea and vomiting. Emetine inhibits protein synthesis in EUKARYOTIC CELLS but not PROKARYOTIC CELLS.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Kinetics: The rate dynamics in chemical or physical systems.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Leucine: An essential branched-chain amino acid important for hemoglobin formation.Enzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.Dexamethasone: An anti-inflammatory 9-fluoro-glucocorticoid.Anisomycin: An antibiotic isolated from various Streptomyces species. It interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system.Pactamycin: Antibiotic produced by Streptomyces pactum used as an antineoplastic agent. It is also used as a tool in biochemistry because it inhibits certain steps in protein synthesis.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Nucleic Acid Synthesis Inhibitors: Compounds that inhibit cell production of DNA or RNA.Depression, Chemical: The decrease in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.TritiumTumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.RNA: A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Chloramphenicol: An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)Tetradecanoylphorbol Acetate: A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.UridineFibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Apomorphine: A derivative of morphine that is a dopamine D2 agonist. It is a powerful emetic and has been used for that effect in acute poisoning. It has also been used in the diagnosis and treatment of parkinsonism, but its adverse effects limit its use.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Colchicine: A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE).Amanitins: Cyclic peptides extracted from carpophores of various mushroom species. They are potent inhibitors of RNA polymerases in most eukaryotic species, blocking the production of mRNA and protein synthesis. These peptides are important in the study of transcription. Alpha-amanitin is the main toxin from the species Amanitia phalloides, poisonous if ingested by humans or animals.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.DEAE-Dextran: Used as a support for ion-exchange chromatography.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Bucladesine: A cyclic nucleotide derivative that mimics the action of endogenous CYCLIC AMP and is capable of permeating the cell membrane. It has vasodilator properties and is used as a cardiac stimulant. (From Merck Index, 11th ed)Ornithine Decarboxylase: A pyridoxal-phosphate protein, believed to be the rate-limiting compound in the biosynthesis of polyamines. It catalyzes the decarboxylation of ornithine to form putrescine, which is then linked to a propylamine moiety of decarboxylated S-adenosylmethionine to form spermidine.Peptide Biosynthesis: The production of PEPTIDES or PROTEINS by the constituents of a living organism. The biosynthesis of proteins on RIBOSOMES following an RNA template is termed translation (TRANSLATION, GENETIC). There are other, non-ribosomal peptide biosynthesis (PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT) mechanisms carried out by PEPTIDE SYNTHASES and PEPTIDYLTRANSFERASES. Further modifications of peptide chains yield functional peptide and protein molecules.Journalism, Dental: Content, management, editing, policies, and printing of dental periodicals such as journals, newsletters, tabloids, and bulletins.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Carbon Isotopes: Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.Deuteroporphyrins: Porphyrins with four methyl and two propionic acid side chains attached to the pyrrole rings.Polyribosomes: A multiribosomal structure representing a linear array of RIBOSOMES held together by messenger RNA; (RNA, MESSENGER); They represent the active complexes in cellular protein synthesis and are able to incorporate amino acids into polypeptides both in vivo and in vitro. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)L Cells (Cell Line): A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.Cytochalasin B: A cytotoxic member of the CYTOCHALASINS.Stimulation, Chemical: The increase in a measurable parameter of a PHYSIOLOGICAL PROCESS, including cellular, microbial, and plant; immunological, cardiovascular, respiratory, reproductive, urinary, digestive, neural, musculoskeletal, ocular, and skin physiological processes; or METABOLIC PROCESS, including enzymatic and other pharmacological processes, by a drug or other chemical.Half-Life: The time it takes for a substance (drug, radioactive nuclide, or other) to lose half of its pharmacologic, physiologic, or radiologic activity.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Methionine: A sulfur-containing essential L-amino acid that is important in many body functions.Rats, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.Erbovirus: A genus in the family PICORNAVIRIDAE causing upper respiratory disease in horses.Receptors, Leukocyte-Adhesion: Family of proteins associated with the capacity of LEUKOCYTES to adhere to each other and to certain substrata, e.g., the C3bi component of complement. Members of this family are the LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN-1; (LFA-1), the MACROPHAGE-1 ANTIGEN; (Mac-1), and the INTEGRIN ALPHAXBETA2 or p150,95 leukocyte adhesion protein. They all share a common beta-subunit which is the CD18 antigen. All three of the above antigens are absent in inherited LEUKOCYTE-ADHESION DEFICIENCY SYNDROME, which is characterized by recurrent bacterial infections, impaired pus formation, and wound healing as well as abnormalities in a wide spectrum of adherence-dependent functions of granulocytes, monocytes, and lymphoid cells.Antimetabolites: Drugs that are chemically similar to naturally occurring metabolites, but differ enough to interfere with normal metabolic pathways. (From AMA Drug Evaluations Annual, 1994, p2033)Aminoisobutyric Acids: A group of compounds that are derivatives of the amino acid 2-amino-2-methylpropanoic acid.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.SKP Cullin F-Box Protein Ligases: A subset of ubiquitin protein ligases that are formed by the association of a SKP DOMAIN PROTEIN, a CULLIN DOMAIN PROTEIN and a F-BOX DOMAIN PROTEIN.Disulfoton: An organothiophosphate insecticide.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.

Bcl-2 and Bcl-XL serve an anti-inflammatory function in endothelial cells through inhibition of NF-kappaB. (1/5972)

To maintain the integrity of the vascular barrier, endothelial cells (EC) are resistant to cell death. The molecular basis of this resistance may be explained by the function of antiapoptotic genes such as bcl family members. Overexpression of Bcl-2 or Bcl-XL protects EC from tumor necrosis factor (TNF)-mediated apoptosis. In addition, Bcl-2 or Bcl-XL inhibits activation of NF-kappaB and thus upregulation of proinflammatory genes. Bcl-2-mediated inhibition of NF-kappaB in EC occurs upstream of IkappaBalpha degradation without affecting p65-mediated transactivation. Overexpression of bcl genes in EC does not affect other transcription factors. Using deletion mutants of Bcl-2, the NF-kappaB inhibitory function of Bcl-2 was mapped to bcl homology domains BH2 and BH4, whereas all BH domains were required for the antiapoptotic function. These data suggest that Bcl-2 and Bcl-XL belong to a cytoprotective response that counteracts proapoptotic and proinflammatory insults and restores the physiological anti-inflammatory phenotype to the EC. By inhibiting NF-kappaB without sensitizing the cells (as with IkappaBalpha) to TNF-mediated apoptosis, Bcl-2 and Bcl-XL are prime candidates for genetic engineering of EC in pathological conditions where EC loss and unfettered activation are undesirable.  (+info)

NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae. (2/5972)

A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. Molecular genetic analysis of NMD3 revealed that it is an essential gene required for stable 60S ribosomal subunits. Cells bearing a temperature-sensitive allele of NMD3 had decreased levels of 60S subunits at the nonpermissive temperature which resulted in the formation of half-mer polysomes. Pulse-chase analysis of rRNA biogenesis indicated that 25S rRNA was made and processed with kinetics similar to wild-type kinetics. However, the mature RNA was rapidly degraded, with a half-life of 4 min. Nmd3p fractionated as a cytoplasmic protein and sedimented in the position of free 60S subunits in sucrose gradients. These results suggest that Nmd3p is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. Putative orthologs of Nmd3p exist in Drosophila, in nematodes, and in archaebacteria but not in eubacteria. The Nmd3 protein sequence does not contain readily recognizable motifs of known function. However, these proteins all have an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization.  (+info)

delta-Aminolevulinate synthetases in the liver cytosol fraction and mitochondria of mice treated with allylisopropylacetamide and 3,5-dicarbethoxyl-1,4-dihydrocollidine. (3/5972)

Hepatic delta-aminolevulinate (ALA) synthetase was induced in mice by the administration of allylisopropylacetamide (AIA) and 3,5-dicarbethoxy-1,4-dihydrocollidine (DDC). In both cases, a significant amount of ALA synthetase accumulated in the liver cytosol fraction as well as in the mitochondria. The apparent molecular weight of the cytosol ALA synthetase was estimated to be 320,000 by gel filtration, but when the cytosol ALA synthetase was subjected to sucrose density gradient centrifugation, it showed a molecular weight of 110,000. In the mitochondria, there were two different sizes of ALA synthetase with molecular weights of 150,000 and 110,000, respectively; the larger enzyme was predominant in DDC-treated mice, whereas in AIA-treated mice and normal mice the enzyme existed mostly in the smaller form. When hemin was injected into mice pretreated with DDC, the molecular size of the mitochondrial ALA synthetase changed from 150,000 to 110,000. The half-life of ALA synthetase in the liver cytosol fraction was about 30 min in both the AIA-treated and DDC-treated mice. The half-life of the mitochondrial ALA synthetase in AIA-treated mice and normal mice was about 60 min, but in DDC-treated mice the half-life was as long as 150 min. The data suggest that the cytosol ALA synthetase of mouse liver is a protein complex with properties very similar to those of the cytosol ALA synthetase of rat liver, which has been shown to be composed of the enzyme active protein and two catalytically inactive binding proteins, and that ALA synthetase may be transferred from the liver cytosol fraction to the mitochondria with a size of about 150,000 daltons, followed by its conversion to enzyme with a molecular weight of 110,000 within the mitochondria. The process of intramitochondrial enzyme degradation seems to be affected in DDC-treated animals.  (+info)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (4/5972)

Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease.  (+info)

Expression of thrombospondin-1 in ischemia-induced retinal neovascularization. (5/5972)

Thrombospondin-1 is an extracellular matrix protein that inhibits endothelial cell proliferation, migration, and angiogenesis. This study was performed to investigate the role of thrombospondin-1 in ischemic retinal neovascularization. In a murine model of retinal neovascularization, thrombospondin-1 mRNA was increased from postnatal day 13 (P13), with a threefold peak response observed on P15, corresponding to the time of development of retinal neovascularization. Prominent expression of thrombospondin-1 was observed in neovascular cells, specifically, cells adjacent to the area of nonperfusion. It has been suggested that vascular endothelial growth factor (VEGF) plays a major role in ischemia-induced retinal neovascularization of this model, so we studied the effects of VEGF on thrombospondin-1 expression. In bovine retinal microcapillary endothelial cells, VEGF induced a biphasic response of thrombospondin-1 expression; VEGF decreased thrombospondin-1 mRNA 0.41-fold after 4 hours, whereas it increased, with a threefold peak response, after 24 hours. VEGF-induced endothelial cell proliferation was completely inhibited by exogenous thrombospondin-1 and increased by 37.5% with anti-thrombospondin-1 antibody. The present findings suggest that, in the ischemic retina, retinal neovascular cells increase thrombospondin-1 expression, and VEGF may stimulate endogenous thrombospondin-1 induction, which inhibits endothelial cell growth. VEGF-mediated thrombospondin-1 induction in ischemia-induced angiogenesis may be a negative feedback mechanism.  (+info)

5'-Nucleotidase activity of mouse peritoneal macrophages. II. Cellular distribution and effects of endocytosis. (6/5972)

The diazonium salt of sulfanilic acid (DASA) can inactivate about 80% of the total 5'-nucleotidase of viable macrophages. The remaining 20% can be inactivated if the cells are first lysed in detergent, and presumably represents an intracellular pool of 5'-nucleotidase. The bulk of this pool may represent cytoplasmic vesicles derived from plasma membrane by endocytosis. This internal compartment is expanded up to threefold immediately after the cells have ingested a large latex load. This is consistent with previous observations on the internalization of 5'-nucleotidase in latex phagosomes. In latex-filled cells this intracellular pool of enzyme is inactivated over a few hours, and the cells then slowly increase their enzyme activity to nearly normal levels. However, 24 h after latex ingestion the metabolism of 5'-nucleotidase in these recovered cells is abnormal, as the rate of enzyme degradation is about twice the normal rate, and the DASA-insensitive enzyme pool in these cells is strikingly diminished. This may reflect effects of the accumulated indigestible particles on the fate of incoming pinocytic vesicles or on newly synthesized plasma membrane precursor. Another endocytic stimulus, concanavalin A, also reduces the total cell 5'-nucleotidase activity. This effect, which is time and temperature dependent, can be prevented by the competitive sugar alpha-methyl mannose. The concanavalin A inhibition can be reversed in the absence of new protein synthesis or in cells cultivated in serum-free conditions. It is not known whether the effect of concanavalin A on 5'-nucleotidase depends upon the interiorizaiton of plasma membrane or is strictly associated with events at the cell surface.  (+info)

CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo. (7/5972)

cAMP activated insertion of the cystic fibrosis transmembrane conductance regulator (CFTR) channels from endosomes to the apical plasma membrane has been hypothesized to regulate surface expression and CFTR function although the physiologic relevance of this remains unclear. We previously identified a subpopulation of small intestinal villus epithelial cells or CFTR high expressor (CHE) cells possessing very high levels of apical membrane CFTR in association with a prominent subapical vesicular pool of CFTR. We have examined the subcellular redistribution of CFTR in duodenal CHE cells in vivo in response to the cAMP activated secretagogue vasoactive intestinal peptide (VIP). Using anti-CFTR antibodies against the C terminus of rodent CFTR and indirect immunofluorescence, we show by quantitative confocal microscopy that CFTR rapidly redistributes from the cytoplasm to the apical surface upon cAMP stimulation by VIP and returns to the cytoplasm upon removal of VIP stimulation of intracellular cAMP levels. Using ultrastructural and confocal immunofluorescence examination in the presence or absence of cycloheximide, we also show that redistribution was not dependent on new protein synthesis, changes in endocytosis, or rearrangement of the apical cytoskeleton. These observations suggest that physiologic cAMP activated apical membrane insertion and recycling of CFTR channels in normal CFTR expressing epithelia contributes to the in vivo regulation of CFTR mediated anion transport.  (+info)

Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide. (8/5972)

A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided.  (+info)

*Cycloheximide

it responds to cycloheximide, so, it should be cultured in a medium free of cycloheximide. Cycloheximide is a highly effective ... Cycloheximide is a eukaryote protein synthesis inhibitor, produced by the bacterium Streptomyces griseus. Cycloheximide exerts ... Cycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein. Treating cells ... Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. ...

*Chlamydosauromyces

It is also cycloheximide resistant. C. punctatus can reproduce both in sexual and asexual forms. The teleomorph phase is ...

*Scedosporium prolificans

This species is sensitive to cycloheximide. As this species may be slow to emerge from clinical materials, specimens in which ...

*Acetoxycycloheximide

Cycloheximide Mahy, Brian W J (2001). A dictionary of virology (3. ed.). San Diego, Calif. [u.a.]: Academic Press. p. 2. ISBN 0 ... It can be considered as the acetylated analogue of cycloheximide. It is a potent Protein synthesis inhibitor in animal cells ...

*Cryptococcus adeliensis

... also grows on 0.01% cycloheximide. Cryptococcus adeliensis sp. nov., a xylanase producing ...

*Streptomyces albulus

... produces acetoxycycloheximide, aciphenol, albanoursin and cycloheximide. Dodd, A.; Swanevelder, D.; ...

*Cyanidioschyzon merolae

Yagisawa F; Nishida K; Okano Y; Minoda A; Tanaka K; Kuroiwa T (2004). "Isolation of cycloheximide-resistant mutants of ...

*Geomyces pannorum

... is resistant to the antifungal agent cycloheximide. However the growth of this species is inhibited by ...

*Eukaryotic ribosome (80S)

Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin". Nat Chem Biol. 6 (3): 209-217. doi: ... One inhibitor of eukaryotic translation elongation is the glutarimide antibiotic cycloheximide (CHX), which was co-crystallized ...

*Lactimidomycin

Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nat Chem Biol. 2010 Mar;6(3):209-217. PMID ...

*Translation (biology)

These include anisomycin, cycloheximide, chloramphenicol, tetracycline, streptomycin, erythromycin, and puromycin. Prokaryotic ...

*Anisomycin

Cycloheximide Anisomycin Archived January 19, 2012, at the Wayback Machine. from Sigma Aldrich Grollman, Arthur P. (1967). " ...

*Alma Joslyn Whiffen-Barksdale

... (October 25, 1916 - July 5, 1981) was a U.S. mycologist who discovered cycloheximide. She was ... List of mycologists Cycloheximide Achlya bisexualis "Alma Whiffen Barksdale (1916-1981)". Smithsonian Institution Archives. ... While there, she discovered the chemical cycloheximide (trade name Actidione), an anti-fungal and anti-bacterial agent produced ...

*Mucor racemosus

It has been shown to adapt to famous antibiotics like cycloheximide, trichodermin and amphotericin B. Cells adapted to ... Shearer G, Jr; Sypherd, PS (March 1988). "Cycloheximide efflux in antibiotic-adapted cells of the fungus Mucor racemosus". ... cycloheximide particularly have been observed to be 40-times more resistant than non-adapted cells to the drug. These adapted ...

*Ribosome profiling

This is commonly performed with cycloheximide but other chemicals can be employed. It is also possible to forgo translation ... RNA-ribosome complexes Cycloheximide Nucleases Phenol/Chloroform Reverse transcriptase dNTPs Sequencing method-cDNA library. ... The other elongating regions can be detected by adding antibiotics like cycloheximide that inhibit translocation, ...

*Harold M. Weintraub

"Conservative segregation of parental histones during replication in the presence of cycloheximide". Proc Natl Acad Sci USA. 76 ...

*C-5 sterol desaturase

Abe Fumiyoshi; Hiraki Toshiki (2009). "Mechanistic role of ergosterol in membrane rigidity and cycloheximide resistance in ...

*Microsporum nanum

Like many other dermatophytes, M. nanum is tolerant of the antifungal agent cycloheximide. In addition, M. nanum also exhibits ...

*Parthenogenesis

Treatment with cycloheximide, a non-specific protein synthesis inhibitor, enhances parthenote development in swine presumably ... "Effects of Cycloheximide on Parthenogenetic Development of Pig Oocytes Activated by Ultrasound Treatment". Journal of ...

*Adrenal ferredoxin

1988). "Human adrenodoxin: cloning of three cDNAs and cycloheximide enhancement in JEG-3 cells". J. Biol. Chem. 263 (7): 3240-4 ...

*Glutarimide

It is a structural component of cycloheximide, a very potent inhibitor of protein synthesis. Cyclohexane Imide Ketone ...

*Cln3

Finally, it was also shown that this delay occurred even with short pulses of cycloheximide, confirming that an unstable ... It was later shown that treatment with the protein synthesis inhibitor cycloheximide delayed Start in yeast, indicating that ... "Inhibition of DNA synthesis in synchronized Chinese hamster cells treated in G1 with cycloheximide". Experimental Cell Research ...

*Bacterivore

By adding cycloheximide, the bacterivores will be inhibited and the bacteria will grow normally. For bacteria adsorbed into ... For experiments with spores (for example spores of C. perfringens), it is not necessary to add cycloheximide to the samples. ... sediment, 2g of cycloheximide per 100g of sediment needs to be added. ...

*Cyclin D1

Baliga BS, Pronczuk AW, Munro HN (Aug 1969). "Mechanism of cycloheximide inhibition of protein synthesis in a cell-free system ... Obrig TG, Culp WJ, McKeehan WL, Hardesty B (Jan 1971). "The mechanism by which cycloheximide and related glutarimide ...

*Trichophyton rubrum

... rubrum cultures can be isolated on both cycloheximide-containing media and cycloheximide-free media. The latter are ... In primary outgrowth on Sabouraud dextrose agar with cycloheximide and antibacterials, contaminating organisms may cause ...
Studies were performed to investigate whether electrically-induced long-term depression (LTD) within rat hippocampal slices in vitro shares any common cellular features with LTD in the intact animal, with particular emphasis being placed on mechanisms required for its late maintenance. Our initial studies have led to the development of stimulation protocols which are able to reliably produce different forms of LTD. Depending on the induction protocol applied, we are able to demonstrate a transient protein synthesis-independent early-LTD with a duration of up to 3-4 h, together with a de novo protein synthesis-dependent late-LTD lasting for at least 8 h. Furthermore, we are able to show input-specific LTD within the CA1 region, with expression shown only by those synapses specifically stimulated by a low-frequency protocol. These studies are important pre-requisites to investigate mechanisms of synaptic tagging and late-associativity during LTD ...
Expression of the HTLV receptor was strictly dependent on de novo protein synthesis. It has been shown that prior to T-lymphocyte proliferation, there is a 7- to 10-fold increase in protein synthesis and a 30- to 40-fold augmentation in mRNA synthesis.33This "pre-proliferation" characteristic appears to be a particularity of T lymphocytes and is not observed in other cell types. The required pre-proliferation burst in mRNA/protein synthesis likely results from the extremely low metabolic rate of resting G0T lymphocytes, which make up the vast majority of the circulating T-lymphocyte pool. Thus, it is intriguing to speculate that it is this low metabolic rate that accounts for the fact that T lymphocytes are the first and only cell type, identified to date, which do not constitutively express the HTLV receptor. Notably, Wodarz and Bangham have recently used a mathematical model to suggest that the rate of evolution of HTLV-1 is limited by the restricted availability of activated uninfected T ...
Medrano, E E. and Pardee, A B., "Prevalent deficiency in tumor cells of cycloheximide-induced cycle arrest." (1980). Subject Strain Bibliography 1980. 3388 ...
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We have investigated the effect of chemotherapeutic and DNA damaging agents on binding of the tumor suppressor phosphoprotein p53 to its consensus DNA sequence. Activation of p53-DNA binding was seen for treatment with radiation, hydrogen peroxide, actinomycin D, Adriamycin, etoposide, camptothecin, 5-fluorouracil, mitomycin C, and cisplatin. These results showed that DNA strand breaks were sufficient to lead to increased levels of p53. The protein synthesis inhibitor cycloheximide blocks the increase in p53 following DNA damage. The increase in p53 activation in camptothecin treated cells may result, at least in part, from an increased half-life of the protein and consequent increases in intracellular protein concentration.. ...
Weigh out the appropriate amount of cycloheximide and add it to the appropriate amount of DMSO to get a 50mg/ml solution. Use the Botstein labs balance as it is much more accurate than ours. It seems to make the most sense to aim for about 100mg (but if it is slightly over or under that is fine) and then put this in a 2ml eppendorf or similar tube (you can use a 15ml conical if you are going to make a larger volume) and add the appropriate amount of DMSO to this based on what you weighed out. Cycloheximide is bad for you (see below) so be careful with it! ...
Fuhr, J E.; Overton, M; and Leisy, M, "Protective effect of cycloheximide upon protein synthesis by l5178y cells exposed to hyperthermia." (1974). Subject Strain Bibliography 1974. 2356 ...
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Process of gene expression entails plasmid DNA or mRNA delivery to specific cells and is a technique routinely utilized in labs to study a gene of interest or a signaling pathway. Gene expression usually allows transient protein production, but in some cases stable integration into a genome and continuous protein production over time is required.
Reference: Polikarpova L.I., Effect of protein synthesis inhibitors on the hormonal induction of alanine and aspartate aminotransferases in the liver of sexually mature male rats, Voprosy meditsinskoi khimii, 1974, vol: 20(2), 215-217 ...
Fertilization of metaphase II-arrested mouse eggs results in resumption of meiosis and a decrease in both cdc2/cyclin B kinase and MAP kinase activities; the decrease in cdc2/cyclin B kinase activity precedes the decrease in MAP kinase activity. Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the fertilization-induced Ca2+ transient. However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase activities that are intimately associated with resumption of meiosis. We report that cycloheximide-treated mouse eggs manifest similar temporal changes in the decrease in both cdc2/cyclin B kinase and MAP kinase activities that occur following fertilization, although cortical granule exocytosis is not stimulated. The decrease in cdc2/cyclin B kinase activity, however, does not seem to be required for the decrease in MAP kinase activity, since the decrease in MAP kinase activity ...
Fig. 5. Identification of Pax3 binding sites on Pax3 promoters in vitro (A) Pax3, but not Zic1 alone, can trigger synthesis of the endogenous Pax3 transcript (primers in 3UTR, not amplifying the inducible form) either in absence of the translation inhibitor cycloheximide (induction is 5-fold compared to uninjected animal caps) or in the presence of cycloheximide (induction is 31-fold, compared to cycloheximide-treated uninjected animal caps). Endogenous pax3 expression level in a stage 11 whole embryo is set at a relative value of 1 unit. After co-injection of Pax3 and Zic1, endogenous pax3 was activated as an immediate-early target as well (77-fold compared to uninjected animal caps; and, in presence of cycloheximide, 16-fold when compared to cycloheximide-treated uninjected animal caps). (B) Location of the ECR containing Pax3 putative binding site in the genomic sequence upstream of the Pax3 TSS. (C) Pax3 binds specifically to the motif identified in the pax3 promoter ECR: an electrophoretic ...
In DG cultures exposed to 10 mM NaCN for 45 min, ≈90% of neurons (identified by immunoreactivity against NeuN, a neuronal marker) died 24 h after hypoxia, because they stained with propidium iodide. Survival of GFAP-immunoreactive glia was not affected (data not shown). Combining the same cyanide treatment with glucose deprivation ("ischemia") had very similar effects on the neurons. By EM, many hypoxic neurons showed early swelling of mitochondria and endoplasmic reticulum, in the presence of an intact nucleus (Fig. 1B), and evolved later toward necrosis with severe cytoplasmic swelling and membrane rupture (Fig. 1C). This finding was in sharp contrast to staurosporine-treated apoptotic neurons, which displayed nuclear breakdown into large, round masses of chromatin (Fig. 1D). Hypoxic necrosis evolved rapidly: we observed a progressive loss of the mitochondrial membrane potential Δψ within a few minutes of cyanide application (Fig. 2 A-D). This Δψ decay was always synchronous with release ...
Treatment with the protein synthesis inhibitor cycloheximide (CHX) or silencing of the core component of the NMD machinery, Upf-1 (also known as Rent1), are widely used to determine if transcripts are subject to NMD (Montfort et al., 2006). CHX is an indirect NMD inhibitor since NMD activation is posttranscriptional, but translation-dependent, and ribosomal association is required during the pioneer round of translation. Upf-1 silencing is believed to produce direct suppression of NMD. The RNA helicase, Upf-1, is a central effector of NMD that links the translation-termination event to the assembly of a surveillance complex. Upf2 and Upf3 are believed to recruit and activate Upf1 on NMD substrates (Lykke-Andersen et al., 2000). Although the discrimination of PTCs from normal termination codons and the molecular links that trigger NMD are still unclear, it is well established that the core components of the NMD machinery are the conserved proteins, Upf-1, Upf-2 and Upf-3. PTC-containing mRNAs ...
Cycloheximide is an antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. Cycloheximide acts by inhibiting elongation during protein synthesis.
A) Western blot demonstrating cycloheximide effects of Wnt-3A mediated accumulation of β-catenin. NCCIT cells were stimulated for 4 hours with CCM (C) or Wnt-3
TNFα, the founding member of the TNF-superfamily, mediates both necroptotic and apoptotic cell death (Walczak, 2011; Micheau and Tschopp, 2003; Andrew, 2004; Wang et al., 2008; Kroemer et al., 2007; Degterev and Yuan, 2008, He et al., 2009; Zhang et al., 2009). Although many investigations have been conducted over the last few years the detailed mechanisms of these death signaling pathways are still not completely known. This particularly concerns our understanding about where ROS are produced intracellularly, how ROS and LMP are mechanistically linked and how both processes contribute to TNFα-induced apoptosis (Boya and Kroemer, 2008; Shen and Pervaiz, 2006; Zdolsek et al., 1993; Brunk et al., 1995). Our study here bridges these missing gaps. We identified that TNFα/CHX-induced LMP is not an apoptosis initiating, but amplifying process downstream of MOMP, and it is triggered by mitochondrial ROS generated as the result of a caspase-3-mediated cleavage of the p75 NDUFS1 subunit of complex I. ...
106. Abstract Protoplasts of Boergesenia forbesii (Hanvey) were treated with inhibitors of protein synthesis in order to investigate their effects on cellulose synthesis. Cellulose synthesis was reversibly inhibited by 10 M cycloheximide as assayed by fluorescence microscopy of Tinopal binding to cellulose. Freeze fracture and image analysis of cycloheximide-treated cells indicated a reduction in the number of intramembrane particles; however, the terminal synthesizing complexes remained at all times. Treatment with 10 M actinomycin D, when applied during the first hour of protoplast formation, irreversibly inhibits cellulose synthesis and terminal complex formation. De novo protein synthesis is required for cell wall regeneration by protoplasts. The data suggest that the structural subunits visualized in the terminal complex do not undergo signifi-cant turnover, but that there may exist an essential proteinaceous component of cellulose synthesis which must be continually renewed ...
Memory can be divided according to different temporal phases: acquisition, consolidation, and retrieval. The consolidation phase is divided into molecular consolidation (range of hours after acquisition) and system consolidation (range of weeks and months after acquisition) (Dudai, 2004). Molecular consolidation is thought to be a protein synthesis-dependent process (Alberini, 2008; Costa-Mattioli et al., 2009). The clear effect of protein synthesis inhibitors on long-term memory was studied extensively mainly in the context of late-phase long-term potentiation (LTP) (Abraham and Williams, 2008). Similar molecular mechanisms were proposed for maintenance of long-term synaptic modifications and learning processes (Davis and Squire, 1984; Costa-Mattioli et al., 2009). However, long-term memory is not supported only by modulation of synaptic strength; modifications in intrinsic neuronal properties also subserve learning-related behavioral changes (Saar and Barkai, 2003; Zhang and Linden, 2003; ...
Summary Treatment of measles virus-infected cells with cycloheximide results in a three-fold increase of 3H-uridine incorporation into the 12 to 36S mRNA species and in the inhibition of genomic 50S RNA synthesis. Consistent with these observations was the finding of a build-up of polyribosomes but an absence of nucleocapsids in the infected cells. These results suggest that measles virus RNA replication, but not transcription, is dependent upon active protein synthesis.
For immunofluorescence, 13-mm-diameter glass coverslips were derivatized for 30 min with 1 mM sulpho-m-maleimidobenzoyl-N-hydrosuccinimide ester (Perbio Science). For biochemical assays, 15-cm tissue culture-treated plastic dishes were coated directly with ligand. Coverslips or dishes were coated for 2 h at room temperature with 10 μg/ml fibronectin polypeptides in Dulbeccos PBS containing calcium and magnesium (Biowhittaker UK) and blocked with 10 mg/ml of heat-denatured BSA for 30 min at room temperature. Equivalent ligand coating between glass and plastic was tested by ELISA using the antifibronectin mAb 333 (Bass et al., 2007). For experiments on defined ligands, cells were treated with 25 μg/ml cycloheximide (Sigma-Aldrich) for 2 h before detachment to prevent de novo matrix synthesis and were then detached with 0.5 mg/ml trypsin. Cells were resuspended in DME/25 mM Hepes and 25 μg/ml cycloheximide, plated at a density of 1.25 × 104 cells per coverslip or 4 × 106 cells per dish, and ...
SR9243 is a potent and selective LXR inverse agonist. SR9243 kills cancer cells by inhibiting lipid production and the Warburg effect. SR9243 induces cell death in multiple types of cancer and does not cause the side effects that have derailed previous attempts to target these processes. In cancer cells, SR9243 significantly inhibited the Warburg effect and lipogenesis by reducing glycolytic and lipogenic gene expression. SR9243 induced apoptosis in tumors without inducing weight loss, hepatotoxicity, or inflammation. Malignant cells exhibit aerobic glycolysis (the Warburg effect) and become dependent on de novo lipogenesis, which sustains rapid proliferation and resistance to cellular stress.
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Buy Anisomycin (CAS 22862-76-6), a protein synthesis inhibitor. Join researchers using high quality Anisomycin from Abcam and achieve your mission, faster.
Cerebellar granule cells are susceptible to the excitotoxin glutamate, which acts at N-methyl-D-aspartate (NMDA) receptors, as well as the neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+), the active cytotoxic metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Paradoxically, preincubation of cultured cerebellar granule cells with low concentrations of NMDA or glutamate markedly antagonizes the neurotoxicity resulting from subsequent exposure to toxic concentrations of either MPP+ or glutamate. The neuroprotective effects of NMDA and glutamate against MPP+ toxicity are observed at agonist concentrations as low as 1 microM, are blocked by specific NMDA receptor antagonists, and require at least 30 min to develop fully. Moreover, NMDA receptor-mediated neuroprotection is prevented by the RNA synthesis inhibitor actinomycin D or the protein synthesis inhibitor cycloheximide. Thus, in cerebellar granule cells activation of NMDA receptors by glutamate can result in either ...
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Rasch et al. recently published an article describing antimicrobial properties of cycloheximide derivatives with adamantyl moieties against Legionella pneumophila. Ten different cycloheximide derivatives were synthesized in this study, and five were found to inhibit L. pneumophila growth at a concentration of 30 uM or 40 uM. Interestingly, several other clinically significant Gram-positive and Gram-negative pathogens were not susceptible to these derivatives which suggests a very narrow spectrum of activity which may be limited specifically to L. pneumophila ...
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Bioprocess intensification can be achieved through high cell density perfusion cell culture with continuous protein capture integration. Protein passage and cell retention are commonly accomplished using tangential flow filtration systems consisting of microporous membranes. Significant challenges, including low efficiency and decaying product sieving over time, are commonly observed in these cell retention devices. Here, we demonstrate that a macroporous membrane overcomes the product sieving challenges when comparing to several other membrane chemistries and pore sizes within the microporous range. Learn More. ...
The mechanism of protein synthesis on 70S ribosomes includes the following stages: 1. The transcription: The process of protein synthesis is started by the uncoiling of strands of DNA molecule. One strand of DNA molecule acts as a template for the formation of mRNA. The mRNA is formed according to the triplet codes of DNA […]. ...
When DArcy Wentworth Thompsons On Growth and Form was published 100 years ago, it raised the question of how biological forms arise during development and across evolution. In light of the advances in molecular and cellular biology since then, a succinct modern view of the question states: how do genes encode geometry? Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales.. Read the Editorial by guest editors Thomas Lecuit and L. Mahadevan, as they provide a perspective on the influence of DArcy Thompsons work and an overview of the articles in this issue.. ...
Figure 5. A, HEC1A cells transfected with LacZ or p85α WT or E160* were treated with cycloheximide (CHX; left) for indicated duration or with MG132 (center) for 24 hours. Cells were then harvested for WB. PTEN levels were normalized to β-actin by densitometry (below). *, P , 0.05. Right, HEC1A cells were transfected with LacZ or WT or E160* in the absence or presence of ubiquitin (Ub) for 72 hours. Whole-cell lysates were collected for IP with anti-PTEN and then subjected to WB with anti-ubiquitin. PTEN protein levels were normalized prior to IP by using proportionally different amounts of lysates. B, left, HEC1A cells were transfected with LacZ or WT or its mutants for 72 hours and were collected for IP with PTEN and WB with anti-p85α. HEC1A cells were co-transfected with WT or increasing amount of E160*. Cell lysates were collected for IP (center) or WB (right top). Right bottom, transfected HEC1A cells were treated with CHX for the indicated time points and harvested for WB. C, cells were ...
This antibiotic originating from the Gram+ actinomycete Streptomyces venezulenza has about the same type of activity as cycloheximide, but than with an ...
Mild hyperthermia is known to enhance apoptosis in a range of normal and neoplastic cell populations. Studies of tumours previously shown to respond to heating in this manner might be expected to provide insights not only into the mechanism of hyperthermic cell killing, but also into the apoptotic process in general. In the present study, cell death induced by 43°C heating for 30 min in two human Burkitts lymphoma lines, BM 13674 and WW1, and in murine mastocytoma P‐815 × 2·1 was found to be exclusively apoptotic in type, identification being based on light and electron microscopic appearances and on the presence of internucleosomal cleavage of DNA into fragments that are multiples of 180-200 base pairs, which was demonstrated by agarose gel electrophoresis. The heat‐induced apoptosis was prevented by the presence of zinc sulphate, an inhibitor of the endonuclease considered to be responsible for the DNA cleavage, but was not suppressed by the protein synthesis inhibitor cycloheximide. ...
An in vitro ischemia model was established and the effect of the metabolic inhibitors cycloheximide (CHX) and actinomycin D (ActD) on apoptosis in astrocytes under ischemia studied. CHX decreased by 75% the number of cells dying after 6 hr of ischemia compared with control cultures. TdT-mediated dUTP nick end labelling (TUNEL) staining of comparable cultures was reduced by 40%. ActD decreased cell death by 60% compared with controls. The number of TUNEL-positive cells was reduced by 38%. The nuclear shrinkage in TUNEL-positive astrocytes in control cultures did not occur in ActD-treated astrocytes, indicating that nuclear shrinkage and DNA fragmentation during apoptosis are two unrelated processes. Expression of bcl-2 (alpha and beta), bax, and Ice in astrocytes under similar ischemic conditions, as measured by quantitative reverse transcription-polymerase chain reaction, indicated that ischemia down-regulated bcl-2 (alpha and beta) and bax. Ice was initially down-regulated from 0 to 4 hr, ...
We used the rat visual cortex as a model system to examine the changes in protein synthesis during experience-induced synaptic plasticity. Dark-rearing rats from birth results in a relatively immature visual cortex that maintains the high de- gree of synaptic plasticity characteristic of the critical period (Kirkwood et al., 1995). Exposure of dark-reared rats to light results in a rapid, robust and coordinated burst of experience- driven synaptic plasticity that can be readily monitored at the biochemical and electrophysiological level (Quinlan et al., 1999). In previous work, we showed that visual experience evokes the polyadenylation of ␣-CaMKII mRNA in visual cortex and the elevation of ␣-CaMKII protein in synaptic fractions from this brain region. Moreover, this increase was a direct result of new synthesis because it was sensitive to the translation inhibitor cycloheximide (Wu et al., 1998). Here we show that the experience-induced increase of ␣-CaMKII pro- tein does not require new ...
Thymidine, a pyrimidine deoxynucleoside, is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. - Mechanism of Action & Protocol.
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When a Walmart Supercenter opened in Winona, Minnesota in 2003, there was definitely a churning among local businesses. Eleven years later, what has been the net effect?
The term protein synthesis refers to the making of a protein. This process begins in the nucleus and then continues in the ribosomes. Protein synthesis is comprised of two parts: DNA transcription...
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BA is a novel antineoplastic agent with cytotoxicity against melanoma and neuroectodermal tumor cells. Here, we report that BA triggers an apoptotic cascade in human malignant glioma cells as well. BA-induced apoptosis of glioma cells involves sequentially new protein synthesis, formation of reactive oxygen species, and caspase processing. In detail, we show that BA toxicity depends on new protein but not mRNA synthesis, suggesting that translation of preexisting mRNA mediates BA toxicity. Caspase 8 and 3 activation are critical steps in the killing cascade triggered by BA because cell death ensues whenever caspase activation is detected. Furthermore, pharmacological (PBN, NAC, DEVD-CHO) or genetic (BCL-2) manipulations that inhibit apoptosis do so at the level of, or upstream of, caspase activation. Cytotoxic, cytokine-resistant glioma cells do not exhibit cross-resistance to BA, suggesting that caspase activation is independent of interactions of endogenous CD95/CD95L or related pairs of death ...
In the present study, we have shown that the PNC actively incorporates Br-UTP and FITC-CTP in a transcription assay using permeabilized cells. Many studies have used this assay to investigate transcription patterns in the cell nucleus (Hozak et al., 1994; Aoki et al., 1997; Fay et al., 1997; Neugebauer and Roth, 1997) since its initial establishment (Jackson et al., 1993; Wansink et al., 1993). Our observation that the PNC actively incorporates labeled nucleotides in a DNA-dependent manner after 5 min of pulse labeling suggests that the PNC is a site of transcription. Inhibition of RNA polymerase I either by addition of actinomycin D in the transcription cocktail or by pretreatment of cells with cycloheximide does not affect the Br-UTP incorporation, indicating that transcription of nascent RNA in the PNC is unlikely to involve RNA polymerase I. This finding is consistent with the report by Matera et al. (1995) that 28S rRNA was not detected in the PNC using in situ hybridization and our ...
I am interested in finding protocols for inhibiting protein and RNA synthesis in vivo in Arabidopsis. Specifically, I am interested in learning the appropritate concentrations of inhibitors such as, but not limited to, cyclohexamide and actinomycin D to use on intact plants, and the time it takes after treatment before one can expect to detect the inhibition. Also, I am interested in protocols that allow one to quantitate the effectiveness of the inhibitors, ie. how to use S35 Met and tritiated uracil to determine if the concentration of the inhibitors and the timing of the treatments were appropriate. Any information will be appreciated. Thanks. Dave Horvath MFT at CLVAX1.CL.MSU.EDU ...
The fundamental, continuing goal of the proposed research is to investigate the potential importance of Ca2+ as a physiologic regulator of protein synthesis in...
Protein synthesis is important because the proteins created during this process control the activities of the cells. Without these proteins, many of the processes in the body would fail or not work...
The mammary gland factor MGF has been described as a developmentally and environmentally regulated nuclear factor required for transcription of the milk protein gene beta-casein. In the current study the individual role of lactogenic hormones in the activation of MGF DNA binding and the functional relation of MGF to known transcription factors was investigated by electrophoretic mobility shift assays. DNA binding of MGF was rapidly induced by PRL in mammary epithelial cells. The activation was not inhibited by the protein synthesis inhibitor cycloheximide. The effect of PRL on MGF did not require costimulation of cells with the other lactogenic hormones, insulin, and glucocorticoids. Thus, MGF is the first example of a nuclear factor directly regulated by PRL. The MGF complexes formed upon initiation of lactation in the mammary gland and upon stimulation of mammary epithelial cells with PRL migrated at the same position in electrophoretic mobility shift assay, whereas the MGF complex found in mammary
The role of heme in the regulation of δ-aminolevulinic acid (ALA) synthetase was studied in fetal rat liver. The activity of ALA synthetase, the first and rate-limiting enzyme in the heme-biosynthetic pathway, is 10 times higher in fetal rat liver mitochondria than in adults. Hemin injection depresses mitochondrial ALA synthetase activity in the adult but not in the fetus. Cycloheximide, a selective inhibitor of protein synthesis in cytoplasmic ribosomes, but not in mitochondria, causes a rapid decrease in fetal mitochondrial ALA synthetase activity. When hemin is given prior to cycloheximide, a slower rate of turnover of mitochondrial ALA synthetase activity occurs than after cycloheximide alone. Treatment with 3-amino-1,2,4-triazole, an inhibitor of δ-aminolevulinic acid dehydratase, the second enzyme in heme biosynthesis, causes a decrease in fetal mitochondrial ALA synthetase activity but no decrease in the extramitochondrial enzyme levels. These studies indicate that heme may facilitate ...
The poliovirus-induced shut-off of cellular protein-synthesis persists in the presence of 3-methylquercetin, a flavonoid which blocks viral protein and RNA-synthesis ...
SGS de novo protein sequencing provides data on a peptides or polypeptides protein sequence when clients have no prior knowledge of it. Find out how it can help your drug discovery or manufacturing processes by contacting us today.
Here, we show that the BH3-only mimetic, ABT-737, has two properties: one, it can overcome resistance to immunotoxin-mediated apoptosis; and two, it can increase immunotoxin delivery from the ER to the cytosol resulting in enhanced killing by as much as 20-fold. Both activities were achieved using concentrations (3 or 10 μmol/L) of ABT-737 that were nontoxic when added alone. Combinations of immunotoxin and ABT-737 were effective at overcoming resistance because each agent targeted a distinct Bcl-2 family member. By inhibiting protein synthesis, immunotoxin treatment promotes a decline in Mcl-1 levels. This was reported previously for both cycloheximide (27) and PE immunotoxins (26), and was confirmed here. ABT-737 is a peptide mimetic modeled on a BH3-only domain and exhibits high binding affinity binding for Bcl-2, Bcl-xl, and Bcl-w (19). Binding of ABT-737 to either Bcl-2 or Bcl-xl neutralizes their prosurvival activity, allowing Bax or Bak to initiate the intrinsic arm of the apoptosis ...
... is the process by which a completed polypeptide is released from the ribosome after the coding information within a messenger ribonucleic acid (mRNA) has been successfully translated
Protein synthesis is one of the most fundamental biological processes to maintain cellular proteostasis. Azidohomoalaine (AHA) is a non-radioactive and
TY - JOUR. T1 - Retinoic acid-induced tissue transglutaminase and apoptosis in vascular smooth muscle cells. AU - Ou, Hesheng. AU - Haendeler, Judith. AU - Aebly, Michael R.. AU - Kelly, Louise A.. AU - Cholewa, Brian C.. AU - Koike, George. AU - Kwitek-Black, Anne. AU - Jacob, Howard J.. AU - Berk, Bradford C.. AU - Miano, Joseph M.. PY - 2000/11/10. Y1 - 2000/11/10. N2 - Retinoids exert antiproliferative and prodifferentiating effects in vascular smooth muscle cells (SMCs) and reduce neointimal mass in balloon-injured blood vessels. The mechanisms through which retinoids carry out these effects are unknown but likely involve retinoid receptor-mediated changes in gene expression. Here we report the cloning, chromosomal mapping, and biological activity of the retinoid-response gene rat tissue transglutaminase (tTG). Northern blotting studies showed that tTG is rapidly and dose-dependently induced in a protein synthesis-independent manner after stimulation with the natural retinoid all-trans ...
Bacterial and Cell Culture Escherichia coli strain DH5α was used for all cloning procedures. E. coli were grown in either LB broth or on LB agar plates supplemented with antibiotics (100 μg/ml ampicillin, 100 μg/ml spectinomycin, and/or 20 μg/ml chloramphenicol) at 30 °C. Mouse L2 fibroblasts were used for cell culture experiments and passage of C. trachomatis L2 434/Bu. Cells were grown in DMEM supplemented with 10 % FBS at 37 °C with 5 % CO2. For routine growth of C. trachomatis, cells were grown until confluent and then infected with EBs via centrifugation at 545g for 1 hour. Chlamydia-infected cells were grown at 37 °C with 5 % CO2 in DMEM, 10 % FBS, 0.2 μg/ml cycloheximide, and 1× non-essential amino acids. EB stocks were titered using the inclusion forming unit assay (IFU) and stored in sucrose phosphate-buffered glutamic acid (SPG, 0.19 mM KH2PO4, 0.36 mM K2HPO4, 0.245 mM l-glutamic acid, 10.9 mM sucrose) at −80 °C. Strains created in this study and specific growth conditions ...
PROTEIN SYNTHESIS Protein synthesis Aspects of protein synthesis Mechanism of protein synthesis (Prokaryotic) Initiation in eukaryotes Translational control and post ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3dec9c-YzFhZ
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That is absurd. Even evolutionists are now agreeing that what they once rejected as impossible, and then grudgingly admitted as "rare," now must be treated as a significant mode of the origins of new genes. As Light, Basile and Elofsson write in their 2014 review which I referenced, "It has even been proposed that the creation of novel genes, a continuous process where most de novo genes are short-lived, is as frequent as gene duplications." Likewise Neme and Tautz explain, "there is now rapidly increasing evidence that de novo evolution of transcripts and genes is not only a theoretical possibility, but might even have been a rather active process throughout evolution." And even Zimmer admitted in the NY Times piece, "Far from being a fluke, these studies suggest that de novo genes are abundant." Nick you have an ax to grind that wont let acknowledge the clear evidence ...
Synthesis of a specific secretory protein was followed in the presence of actinomycin D and cordycepin (3-deoxyadenosine). The apparent half-life of the messenger RNA for this protein is significantly longer in the presence of actinomycin D than in the presence of cordycepin. These results suggest that actinomycin D studies of half-life in specialized cells may be inaccurate. ...
BERKELEY, Calif. (MarketWatch) -- One of the weird ironies we are witnessing is the reaction to Yahoo Inc. turning over information about a user in China to...
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TY - GEN. T1 - Cycloheximide reduces retinal ganglion cell death induced by tectal ablation in neonatal rats. AU - Harvey, Alan. AU - Cui, Q.. AU - Robertson, Donald. PY - 1993. Y1 - 1993. M3 - Conference paper. VL - 4. SP - 36. EP - 36. BT - Australian Neuroscience Society Meeting. A2 - Powis, D.. PB - Australian Neuroscience Society. CY - Melbourne. ER - ...
We investigated the effect of systemic hypoxia (Krebs-Henseleit solution gassed with 5% CO2/95% N2) on an isolated, perfused rat lung. Hypoxia resulted in a slowly developing sustained increase in pulmonary perfusion pressure (PPP) accompanied by an increase in lung weight (LW). The endothelin (ET) receptor antagonists BQ123 (3 and 10 μM), BQ788 (3 μM) and bosentan (1.5 and 5 μM) all attenuated the hypoxia-induced increases in LW and PPP. In addition, phosphoramidon (1 μM), an ET-converting enzyme inhibitor, also significantly attenuated the hypoxia-induced increases in PPP and LW. The use of two agents that alter peptide secretion, phalloidin (10 and 50 nM) and colchicine (100 nM), and the peptide synthesis inhibitor cycloheximide (5 μM) all significantly attenuated the hypoxia-induced increases in PPP and LW. The increase in PPP and LW after the onset of hypoxia was accompanied by an increase in perfusate levels of ET-1 compared with normoxic time-matched controls. The results show that ...
AB - In the present work, a workflow on the development of a continuous protein crystallisation is introduced, with lysozyme as a model protein, from micro L screening experiments, to small scale batch crystallisation experiments in a shaking crystallisation platform, and to batch and continuous crystallisation experiments in an oscillatory flow platform. The lysozyme crystallisation investigated were for a concentration range from 30 to 100 mg/mL, shaking conditions from 100 to 200 rpm in the batch shaking crystallisation platform, and oscillatory conditions with amplitude (x) from 5 to 30 mm and frequency (f) from 0.1 to 1.0 Hz in the batch oscillatory flow crystallisation platform. We propose the use of the Reynolds number (Re) for scaling up of the process from the shaking batch to the continuous oscillatory flow platform. Additionally, it is shown that the nucleation rate increased with increase in concentration of initial lysozyme solution, or increase in shear rate, inducing smaller size ...
Actinomycin D, Calcium, Concentration, Ethanol, Microelectrodes, Actinomycin, Aldosterone, Blood, Capillaries, Capillary, Cycloheximide, Drugs, Gene, Glucocorticoid Receptor, Kidney, Luminal, Mineralocorticoid Receptor, Perfusion, Rat, Ru 486
Hello all. I do not post here regularly but I am interested in the same kind of questions Dave asks. How can homebrewers best start to understand brett strains as well as we do saccharomyces strains?. One simple way may be to get brett cultures not from Wyeast or White Labs but by culturing from bottles. If we can do this, then we also have the ability to select brett from beers we like, and to compare our results to the commercial versions and maybe learn something from whatever differences arise.. Those of us who dont get into agar plates and cyclohexamide and all of that, have some options. The best option might be to simply step up the dregs of a beer that is known to have only a single strain of brett, and no saccharomyces, in the bottle. Anyone know if this is true of Orval? Rainaert Flemish Wild Ale? The soon-to-be-released Boulevard Saison?. Almost as good would be to step up the dregs of beers that have only a single strain of brett and a single strain of saccharomyces. When such a ...
Photo sequence showing how a beetle (Pleocoma) rights itself. Sequence includes photo numbers: BK0711, BK0712, BK0713, BK0714, BK0715, and BK0716. - Stock Image C003/9974
Quinoa is high in protein. It also contains phytochemicals that increase protein synthesis, protect against cancer and lower blood glucose
Once the code has been completely read, a stop signal is given and protein synthesis is complete and the protein goes where it is ...
Genes must be expressed in precise levels and at the exact moment if the complex balance regulating cell activity is to be maintained. Messenger RNA (mRNA) conveys genetic information from DNA to ribosomes, where proteins ...
organism. One instance that exemplifies this argument is that cells can produce protein. It isnt as simple as just making protein, and it involves
Chronic lymphocytic leukemia (CLL) is the most prevalent lymphoid malignancy in the elderly of the Western world. Although treatment options have improved over the past two decades, 10-15% of patients still have a poor prognosis and are often resistant to therapy. Aberrations in the p53 pathway, such as a deleted (del17p13) or mutated p53 gene, are highly enriched in this class of patients. In an extensive screen for p53-independent apoptosis inducers, actinomycin D was identified from 1496 substances and shown to induce apoptosis in primary CLL cells derived from high-risk patients including those with aberrant p53, revealing a novel p53-independent mechanism of action. Both pro-survival genes BCL2 and MCL1 are targeted by actinomycin D, in contrast to fludarabine the backbone of current treatment schedules. In the well-established TCL1 transgenic mouse model for high-risk CLL, actinomycin D treatment was more effective in reducing tumor load than fludarabine, with no evidence of resistance after three
The rat liver nucleolus, after fragmentation induced by ethionine treatment, has been found to undergo complete reformation by adenine in the presence of a dose of cycloheximide sufficient to cause inhibition of protein synthesis by 90-95%. In contrast, actinomycin D given along with adenine was followed by the appearance of a small compact mass containing only the fibrillar component with no evident granules. This structure resembled pseudonucleoli seen in the anucleolate mutant of Xenopus laevis or in certain early stages of amphibian oocytes. Actinomycin D administered 2 hr after adenine induced a segregation of the fibrillar and granular components of nucleoli similar to that induced in the normal nucleolus. The implications of these findings in relation to nucleolar organization are briefly discussed.. ...
In phytopathogenic fungi, the expression of hundreds of small secreted protein (SSP)-encoding genes is induced upon primary infection of plants while no or a low level of expression is observed during vegetative growth. In some species such as Leptosphaeria maculans, this coordinated in-planta upregulation of SSP-encoding genes expression relies on an epigenetic control but the signals triggering gene expression in-planta are unknown. In the present study, biotic and abiotic factors that may relieve suppression of SSP-encoding gene expression during axenic growth of L. maculans were investigated. Some abiotic factors (temperature, pH) could have a limited effect on SSP gene expression. In contrast, two types of cellular stresses induced by antibiotics (cycloheximide, phleomycin) activated strongly the transcription of SSP genes. A transcriptomic analysis to cycloheximide exposure revealed that biological processes such as ribosome biosynthesis and rRNA processing were induced whereas important metabolic
Ford Mercury not turning over, not making any noises like clicking sounds or anything - I just bought a 2002 Ford Mercury about a month ago. I drove it abo...
Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protoco
I decided to get myself an electric car- the Nissan Leaf. Im happy to support the electric car technology and reduce my carbon footprint. I bought the leaf because its 100 mile range, all electric engine, and roomy backseat serve my needs best. I ordered one fully loaded, just as my Prius and ford fusion…
[Role of protein synthesis in the process of degradation of anomalous proteins in Escherichia coli cells].: The degradation of three types of anomalous proteins
Protein Synthesis What is protein synthesis? Is protein synthesis important? What is RNA? Where is RNA found? Is RNA similar to DNA? What is translation?

Cycloheximide - WikipediaCycloheximide - Wikipedia

"Cycloheximide - The Antimicrobial Index Knowledgebase - TOKU-E". antibiotics.toku-e.com.. *^ "Cycloheximide-Naramycin A; ... Cycloheximide is a eukaryote protein synthesis inhibitor, produced by the bacterium Streptomyces griseus. Cycloheximide exerts ... it responds to cycloheximide, so, it should be cultured in a medium free of cycloheximide. ... Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. ...
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cycloheximide (CHEBI:27641)cycloheximide (CHEBI:27641)

... is a piperidones (CHEBI:48589) cycloheximide (CHEBI:27641) is a secondary alcohol (CHEBI:35681) ... cycloheximide (CHEBI:27641) is a cyclic ketone (CHEBI:3992) cycloheximide (CHEBI:27641) is a dicarboximide (CHEBI:35356) ... cycloheximide (CHEBI:27641) has functional parent piperidine-2,6-dione (CHEBI:5435) cycloheximide (CHEBI:27641) has role ... cycloheximide (CHEBI:27641) has role protein synthesis inhibitor (CHEBI:48001) cycloheximide (CHEBI:27641) is a antibiotic ...
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Weigh out the appropriate amount of cycloheximide and add it to the appropriate amount of DMSO to get a 50mg/ml solution. Use ... Cycloheximide (MP Biomedicals, Cat #100183). *DMSO (Fluka 41639, Ultra for molecular biology; stored in the Flammables cabinet) ... Cycloheximide is bad for you (see below) so be careful with it! ... Megan N McClean: Cycloheximide is BAD for you. Check out the ... Each person in the lab makes up their own stock of cycloheximide and keeps it in their own -20°C box. ...
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Reduction of Rat Liver Microsomal Ribonuclease by Cycloheximide | Molecular PharmacologyReduction of Rat Liver Microsomal Ribonuclease by Cycloheximide | Molecular Pharmacology

Reduction of Rat Liver Microsomal Ribonuclease by Cycloheximide Message Subject (Your Name) has forwarded a page to you from ... The results indicate that the short onset of microsomal RNase reduction produced by cycloheximide may be due to a more rapid ... Reduction of Rat Liver Microsomal Ribonuclease by Cycloheximide. ROBERT T. LOUIS-FERDINAND ... Reduction of Rat Liver Microsomal Ribonuclease by Cycloheximide. ROBERT T. LOUIS-FERDINAND ...
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Cycloheximide, Crystalline | Krackeler Scientific, Inc.Cycloheximide, Crystalline | Krackeler Scientific, Inc.

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A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina | DevelopmentA propos d'un effet paradoxal de la cycloheximide sur l'œuf d'Artemia salina | Development

Cycloheximide, an inhibitor of protein synthesis at the translation level, has a very strong antimitotic activity upon the ... A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina ... A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina ... A propos dun effet paradoxal de la cycloheximide sur lœuf dArtemia salina ...
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Effects of cycloheximide on gene expression changes in  | Open-iEffects of cycloheximide on gene expression changes in | Open-i

Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were allowed to attach ... pone.0129578.g008: Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were ... pone.0129578.g008: Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were ... was blocked by cycloheximide (Fig 7). The expressions of Wnt target genes, such as LEF1 and Cyc-D1, and several growth factors ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC4462586_pone.0129578.g008&req=4

Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after...Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after...

Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after ... No significant reduction in the probability of the conditioned response in cycloheximide-treated bees was found when compared ... Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after ... Inhibition of brain protein synthesis by cycloheximide does not affect formation of long-term memory in honeybees after ...
more infohttp://www.jneurosci.org/content/13/4/1379

Cycloheximide prevents the  de novo  polypeptide synthesis required to recover from acetylene inhibition in  Nitrosopumilus...Cycloheximide prevents the de novo polypeptide synthesis required to recover from acetylene inhibition in Nitrosopumilus...

Cycloheximide prevents the de novo polypeptide synthesis required to recover from acetylene inhibition in Nitrosopumilus ... However, cycloheximide (CHX), a widely used eukaryotic protein synthesis inhibitor, but not bacteria-specific protein synthesis ...
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Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities | Journal of Cell ScienceCycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities | Journal of Cell Science

We report that cycloheximide-treated mouse eggs manifest similar temporal changes in the decrease in both cdc2/cyclin B kinase ... Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the ... However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase ... Cycloheximide-induced activation of mouse eggs: effects on cdc2/cyclin B and MAP kinase activities ...
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Learn more about cycloheximide. We enable science by offering product choice, services, process excellence and our people make ... Description: Cycloheximide is a glutarimide antibiotic derived from a microbial source. Cycloheximide is an antibiotic which is ... Description: CYCLOHEXIMIDE, also known as hizarocin, is used in biomedical research. It inhibits protein synthesis in ... Description: Cycloheximide, Purity: 90%, CAS Number 66-81-9, Formula: C15H23NO4, Synonyms: 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)- ...
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Cycloheximide acts by inhibiting elongation during protein synthesis. ... Cycloheximide is an antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. ... Cycloheximide is an antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. Cycloheximide ... Cycloheximide is a colorless crystals. Used as a fungicide and as a anticancer drug. ...
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The Effect of Cycloheximide on the Replication of Measles Virus | Microbiology SocietyThe Effect of Cycloheximide on the Replication of Measles Virus | Microbiology Society

Summary Treatment of measles virus-infected cells with cycloheximide results in a three-fold increase of 3H-uridine ... The Effect of Cycloheximide on the Replication of Measles Virus * W. W. Hall, D. Genius and V. ter Meulen ... Treatment of measles virus-infected cells with cycloheximide results in a three-fold increase of 3H-uridine incorporation into ...
more infohttps://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-35-3-579

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Novel cycloheximide derivatives with antimicrobial activity against Legionella pneumophilaNovel cycloheximide derivatives with antimicrobial activity against Legionella pneumophila

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A) Western blot demonstrating cycloheximide effects of  | Open-iA) Western blot demonstrating cycloheximide effects of | Open-i

Western blot demonstrating cycloheximide effects of Wnt-3A mediated accumulation of β-catenin. NCCIT cells were stimulated for ... of cycloheximide. Note that β-catenin does not accumulate in response to Wnt-3A CM when cycloheximide is present. B) Northern ... of cycloheximide. Note that β-catenin does not accumulate in response to Wnt-3A CM when cycloheximide is present. B) Northern ... or absence of cycloheximide. As expected, BMP-4 mediated induction of MSX2 is not affected by cycloheximide which contrasts ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC117803_1471-213X-2-8-4&req=4

Human adrenodoxin: Cloning of three cDNAs and cycloheximide enhancement in JEG-3 cells<...Human adrenodoxin: Cloning of three cDNAs and cycloheximide enhancement in JEG-3 cells<...

Addition of cycloheximide or puromycin to such cultures substantially reduced basal levels and markedly attenuated the cAMP- ... Addition of cycloheximide or puromycin to such cultures substantially reduced basal levels and markedly attenuated the cAMP- ... Addition of cycloheximide or puromycin to such cultures substantially reduced basal levels and markedly attenuated the cAMP- ... Addition of cycloheximide or puromycin to such cultures substantially reduced basal levels and markedly attenuated the cAMP- ...
more infohttps://kyushu-u.pure.elsevier.com/ja/publications/human-adrenodoxin-cloning-of-three-cdnas-and-cycloheximide-enhanc

Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtiiCycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii

... Stevens, DR ... Chlamydomonas, Cycloheximide resistance, Ribosomal protein L41, Intron National Category Medical Biotechnology (with a focus on ... Although most eukaryotic cells are sensitive to the 80S ribosome inhibitor cycloheximide (CYH), naturally occurring CYH ...
more infohttp://hh.diva-portal.org/smash/record.jsf?pid=diva2:239096

Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtiiCycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii

... Stevens, DR ... Chlamydomonas, Cycloheximide resistance, Ribosomal protein L41, Intron National Category Medical Biotechnology (with a focus on ... Although most eukaryotic cells are sensitive to the 80S ribosome inhibitor cycloheximide (CYH), naturally occurring CYH ...
more infohttp://hh.diva-portal.org/smash/record.jsf?faces-redirect=true&language=en&searchType=SIMPLE&query=&af=%5B%5D&aq=%5B%5B%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&pid=diva2%3A239096&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all
  • Effects of cycloheximide on gene expression changes in DPCs induced by oleuropein treatment.DPCs were allowed to attach overnight and then treated (60 min) with cycloheximide (10 μg/ml) and thereafter switched to medium with or without oleuroepin (20 μM) in the presence of cycloheximide (10 μg/ml) for 24 h. mRNA expression levels of LEF1, Cyc-D1, IGF-1, HGF, VEGF, and KGF were measured by RT-PCR. (nih.gov)
  • Treatment of measles virus-infected cells with cycloheximide results in a three-fold increase of 3 H-uridine incorporation into the 12 to 36S mRNA species and in the inhibition of genomic 50S RNA synthesis. (microbiologyresearch.org)
  • Addition of cycloheximide or puromycin to such cultures substantially reduced basal levels and markedly attenuated the cAMP-induced accumulation of cytochrome P450scc mRNA, but augmented the accumulation of adrenodoxin and fos mRNAs in additive and multiplicative fashions, respectively. (elsevier.com)
  • Cycloheximide (CH) was added to infected cultures to accumulate early viral mRNA relative to host cell mRNA. (ias.ac.in)
  • Weigh out the appropriate amount of cycloheximide and add it to the appropriate amount of DMSO to get a 50mg/ml solution. (openwetware.org)
  • The exposure of HeLa cells to interleukin-1 alpha (IL-1α) in the presence of cycloheximide (CHX) leads to the release of active tumor necrosis factor alpha (TNF-α), eliciting cytocidal effect on these cells. (edu.pl)
  • Prevalent deficiency in tumor cells of cycloheximide-induced cycle arrest. (jax.org)
  • Cycloheximide has also been reported to inhibit FKBP12 (peptidylprolylisomerase hFKBP12, PPIase hFKBP12) via competitive inhibition. (wikipedia.org)
  • As expected, BMP-4 mediated induction of MSX2 is not affected by cycloheximide which contrasts with the inhibition of Wnt-3A mediated induction of MSX2 in the presence of cycloheximide. (nih.gov)
  • Cycloheximide has also been used for its antifungal properties to make isolation of bacteria from mixed samples easier. (wikipedia.org)
  • Note that β-catenin does not accumulate in response to Wnt-3A CM when cycloheximide is present. (nih.gov)
  • The results indicate that the short onset of microsomal RNase reduction produced by cycloheximide may be due to a more rapid turnover of this enzyme in comparison with other microsomal proteins. (aspetjournals.org)
  • No significant reduction in the probability of the conditioned response in cycloheximide-treated bees was found when compared to the Ringer-injected controls in 4 series of experiments. (jneurosci.org)
  • The decrease in cdc2/cyclin B kinase activity, however, does not seem to be required for the decrease in MAP kinase activity, since the decrease in MAP kinase activity still occurs in cycloheximide-treated eggs that are also incubated in the presence of nocodazole, which inhibits cyclin B degradation and hence the decrease in cdc2/cyclin B kinase. (biologists.org)
  • NCCIT cells were stimulated for 4 hours with CCM (C) or Wnt-3A CM (Wnt) in the presence (20 microgram/ml) or absence (0) of cycloheximide. (nih.gov)
  • B) Northern analysis demonstrating that MSX2 induction by Wnt-3A is abolished in the presence of cycloheximide. (nih.gov)
  • Due to significant toxic side effects, including DNA damage , teratogenesis , and other reproductive effects (including birth defects and toxicity to sperm ), cycloheximide is generally used only in in vitro research applications, and is not suitable for human use as a therapeutic compound. (wikipedia.org)
  • A) Western blot demonstrating cycloheximide effects of Wnt-3A mediated accumulation of β-catenin. (nih.gov)
  • Graphical analysis of the time plot of ribonuclease activity decay following cycloheximide administration indicated that the half-life of the microsomal ribonuclease was 54 min. (aspetjournals.org)
  • InSolution™ Cycloheximide, CAS 66-81-9, is a 100 mg/ml, sterile-filtered solution of Cycloheximide (Cat. (emdmillipore.com)
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  • Using a chronically implanted cannula which will be described, a solution of cycloheximide (CHX) at a dose of 0.2 mg/kg was applied to the pla mater overlying the forelimb sensorimotor cortex. (sussex.ac.uk)
  • Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the fertilization-induced Ca2+ transient. (biologists.org)
  • However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase activities that are intimately associated with resumption of meiosis. (biologists.org)
  • Our results showed that the accumulation of β-catenin by oleuropein, as determined by immunocytochemistry, was blocked by cycloheximide (Fig 7). (nih.gov)
  • Translation is halted via the addition of cycloheximide, and the DNA/RNA in the cell is then nuclease treated. (wikipedia.org)
  • This is diluted to 100μg/mL to inhibit translation (though you should check this concentration does what you think it does whenever you develop a new protocol using cycloheximide). (openwetware.org)
  • it responds to cycloheximide, so, it should be cultured in a medium free of cycloheximide. (wikipedia.org)
  • Cycloheximide and actinomycin D delay death and affect bcl-2, bax, and ice gene expression in astrocytes under in vitro ischemia[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2003,74(2):318-325. (bjmu.edu.cn)
  • The synthesis of this factor could be extremely sensitive to cycloheximide action. (biologists.org)