A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
A cyclin subtype that is transported into the CELL NUCLEUS at the end of the G2 PHASE. It stimulates the G2/M phase transition by activating CDC2 PROTEIN KINASE.
Cdh1 is an activator of the anaphase-promoting complex-cyclosome, and is involved in substrate recognition. It associates with the complex in late MITOSIS from anaphase through G1 to regulate activity of CYCLIN-DEPENDENT KINASES and to prevent premature DNA replication.
A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.
A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. They generally function as ENZYME ACTIVATORS that drive the CELL CYCLE through transitions between phases. A subset of cyclins may also function as transcriptional regulators.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
Complexes of enzymes that catalyze the covalent attachment of UBIQUITIN to other proteins by forming a peptide bond between the C-terminal GLYCINE of UBIQUITIN and the alpha-amino groups of LYSINE residues in the protein. The complexes play an important role in mediating the selective-degradation of short-lived and abnormal proteins. The complex of enzymes can be broken down into three components that involve activation of ubiquitin (UBIQUITIN-ACTIVATING ENZYMES), conjugation of ubiquitin to the ligase complex (UBIQUITIN-CONJUGATING ENZYMES), and ligation of ubiquitin to the substrate protein (UBIQUITIN-PROTEIN LIGASES).
An E3 ubiquitin ligase primarily involved in regulation of the metaphase-to-anaphase transition during MITOSIS through ubiquitination of specific CELL CYCLE PROTEINS. Enzyme activity is tightly regulated through subunits and cofactors, which modulate activation, inhibition, and substrate specificity. The anaphase-promoting complex, or APC-C, is also involved in tissue differentiation in the PLACENTA, CRYSTALLINE LENS, and SKELETAL MUSCLE, and in regulation of postmitotic NEURONAL PLASTICITY and excitability.
A cyclin G subtype that is constitutively expressed throughout the cell cycle. Cyclin G1 is considered a major transcriptional target of TUMOR SUPPRESSOR PROTEIN P53 and is highly induced in response to DNA damage.
A cyclin subtype that is found associated with CYCLIN-DEPENDENT KINASE 5; cyclin G associated kinase, and PROTEIN PHOSPHATASE 2.
Protein encoded by the bcl-1 gene which plays a critical role in regulating the cell cycle. Overexpression of cyclin D1 is the result of bcl-1 rearrangement, a t(11;14) translocation, and is implicated in various neoplasms.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
A cyclin subtype that has specificity for CDC2 PROTEIN KINASE and CYCLIN-DEPENDENT KINASE 2. It plays a role in progression of the CELL CYCLE through G1/S and G2/M phase transitions.
A lipid phosphatase that acts on phosphatidylinositol-3,4,5-trisphosphate to regulate various SIGNAL TRANSDUCTION PATHWAYS. It modulates CELL GROWTH PROCESSES; CELL MIGRATION; and APOPTOSIS. Mutations in PTEN are associated with COWDEN DISEASE and PROTEUS SYNDROME as well as NEOPLASTIC CELL TRANSFORMATION.
Tumors or cancer of ENDOMETRIUM, the mucous lining of the UTERUS. These neoplasms can be benign or malignant. Their classification and grading are based on the various cell types and the percent of undifferentiated cells.
A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.
A cell line derived from cultured tumor cells.
Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.
A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The interactions of particles responsible for their scattering and transformations (decays and reactions). Because of interactions, an isolated particle may decay into other particles. Two particles passing near each other may transform, perhaps into the same particles but with changed momenta (elastic scattering) or into other particles (inelastic scattering). Interactions fall into three groups: strong, electromagnetic, and weak. (From McGraw-Hill Encyclopedia of Science & Technology, 7th ed)
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.
A family of transcription factors that share a unique DNA-binding domain. The name derives from viral oncogene-derived protein oncogene protein v-ets of the AVIAN ERYTHROBLASTOSIS VIRUS.
A heterotetrameric transcription factor composed of two distinct proteins. Its name refers to the fact it binds to DNA sequences rich in GUANINE and ADENINE. GA-binding protein integrates a variety of SIGNAL TRANSDUCTION PATHWAYS and regulates expression of GENES involved in CELL CYCLE control, PROTEIN BIOSYNTHESIS, and cellular METABOLISM.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
An inorganic and water-soluble platinum complex. After undergoing hydrolysis, it reacts with DNA to produce both intra and interstrand crosslinks. These crosslinks appear to impair replication and transcription of DNA. The cytotoxicity of cisplatin correlates with cellular arrest in the G2 phase of the cell cycle.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
A group of 4-keto-FLAVONOIDS.

Control of cell cycle progression by c-Jun is p53 dependent. (1/94)

The c-jun proto-oncogene encodes a component of the mitogen-inducible immediate-early transcription factor AP-1 and has been implicated as a positive regulator of cell proliferation and G1-to-S-phase progression. Here we report that fibroblasts derived from c-jun-/- mouse fetuses exhibit a severe proliferation defect and undergo a prolonged crisis before spontaneous immortalization. The cyclin D1- and cyclin E-dependent kinases (CDKs) and transcription factor E2F are poorly activated, resulting in inefficient G1-to-S-phase progression. Furthermore, the absence of c-Jun results in elevated expression of the tumor suppressor gene p53 and its target gene, the CDK inhibitor p21, whereas overexpression of c-Jun represses p53 and p21 expression and accelerates cell proliferation. Surprisingly, protein stabilization, the common mechanism of p53 regulation, is not involved in up-regulation of p53 in c-jun-/- fibroblasts. Rather, c-Jun regulates transcription of p53 negatively by direct binding to a variant AP-1 site in the p53 promoter. Importantly, deletion of p53 abrogates all defects of cells lacking c-Jun in cell cycle progression, proliferation, immortalization, and activation of G1 CDKs and E2F. These results demonstrate that an essential, rate-limiting function of c-Jun in fibroblast proliferation is negative regulation of p53 expression, and establish a mechanistic link between c-Jun-dependent mitogenic signaling and cell-cycle regulation.  (+info)

Altered regulation of cyclin G in human breast cancer and its specific localization at replication foci in response to DNA damage in p53+/+ cells. (2/94)

Cyclin G, a recent addition to the cyclin family, was initially identified in screens for new src kinase family members and soon thereafter by differential screening for transcriptional targets of the tumor suppressor gene, p53. We have identified cyclin G as being overexpressed in breast and prostate cancer cells using differential display polymerase chain reaction screening. We demonstrate here that cyclin G is overexpressed in human breast and prostate cancer cells and in cancer cells in situ from tumor specimens. Cyclin G expression was tightly regulated throughout the cell cycle in normal breast cells, peaking at the S and G2/M phases of the cell cycle with lower levels in G1. The cell cycle-dependent expression was absent in breast cancer cells. Following DNA damage in normal p53+/+ cells, cyclin G is triggered to cluster in discrete nuclear DNA replication foci that contain replication-associated proteins such as proliferating cell nuclear antigen (PCNA). While p53-/- cells displayed a faint cyclin G nuclear staining pattern, there was no increased expression and no change in distribution of the staining pattern after DNA damage. The specific subcellular localization of cyclin G at DNA replication foci provides an additional link between p53-mediated growth arrest and cell cycle regulation and suggests that cyclin G may act as an effector of p53-mediated events by functional association with replication foci protein(s).  (+info)

G1 checkpoint protein and p53 abnormalities occur in most invasive transitional cell carcinomas of the urinary bladder. (3/94)

The G1 cell cycle checkpoint regulates entry into S phase for normal cells. Components of the G1 checkpoint, including retinoblastoma (Rb) protein, cyclin D1 and p16INK4a, are commonly altered in human malignancies, abrogating cell cycle control. Using immunohistochemistry, we examined 79 invasive transitional cell carcinomas of the urinary bladder treated by cystectomy, for loss of Rb or p16INK4a protein and for cyclin D1 overexpression. As p53 is also involved in cell cycle control, its expression was studied as well. Rb protein loss occurred in 23/79 cases (29%); it was inversely correlated with loss of p16INK4a, which occurred in 15/79 cases (19%). One biphenotypic case, with Rb+p16- and Rb-p16+ areas, was identified as well. Cyclin D1 was overexpressed in 21/79 carcinomas (27%), all of which retained Rb protein. Fifty of 79 tumours (63%) showed aberrant accumulation of p53 protein; p53 staining did not correlate with Rb, p16INK4a, or cyclin D1 status. Overall, 70% of bladder carcinomas showed abnormalities in one or more of the intrinsic proteins of the G1 checkpoint (Rb, p16INK4a and cyclin D1). Only 15% of all bladder carcinomas (12/79) showed a normal phenotype for all four proteins. In a multivariate survival analysis, cyclin D1 overexpression was linked to less aggressive disease and relatively favourable outcome. In our series, Rb, p16INK4a and p53 status did not reach statistical significance as prognostic factors. In conclusion, G1 restriction point defects can be identified in the majority of bladder carcinomas. Our findings support the hypothesis that cyclin D1 and p16INK4a can cooperate to dysregulate the cell cycle, but that loss of Rb protein abolishes the G1 checkpoint completely, removing any selective advantage for cells that alter additional cell cycle proteins.  (+info)

Ectopic expression of Cdc25A accelerates the G(1)/S transition and leads to premature activation of cyclin E- and cyclin A-dependent kinases. (4/94)

Human Cdc25 phosphatases play important roles in cell cycle regulation by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases. Three human Cdc25 isoforms, A, B, and C, have been discovered. Cdc25B and Cdc25C play crucial roles at the G(2)/M transition. In the present study, we have investigated the function of human Cdc25A phosphatase. Cell lines that express human Cdc25A in an inducible manner have been generated. Ectopic expression of Cdc25A accelerates the G(1)/S-phase transition, indicating that Cdc25A controls an event(s) that is rate limiting for entry into S phase. Furthermore, we carried out a detailed analysis of the expression and activation of human Cdc25A. Activation of endogenous Cdc25A occurs during late G(1) phase and increases in S and G(2) phases. We further demonstrate that Cdc25A is activated at the same time as cyclin E- and cyclin A-dependent kinases. In vitro, Cdc25A dephosphorylates and activates the cyclin-Cdk complexes that are active during G(1). Overexpression of Cdc25A in the inducible system, however, leads to a premature activation of both cyclin E-Cdk2 and cyclin A-Cdk2 complexes, while no effect of cyclin D-dependent kinases is observed. Furthermore, Cdc25A overexpression induces a tyrosine dephosphorylation of Cdk2. These results suggest that Cdc25A is an important regulator of the G(1)/S-phase transition and that cyclin E- and cyclin A-dependent kinases act as direct targets.  (+info)

A role of cyclin G in the process of apoptosis. (5/94)

Cyclin G was previously identified as a target gene of the p53 tumor suppresser protein, and levels of cyclin G are increased after induction of p53 by DNA damage. However, the function of cyclin G has not been established. To determine the effect of increased expression of cyclin G, retroviruses encoding cyclin G were constructed and used to infect three different murine cell lines. Cyclin G protein levels induced by the retroviruses were within the range seen after DNA damage induction of p53. In each case we observed that such over-expression of cyclin G augments the apoptotic process. TNF-alpha induction of apoptosis is increased by expression of cyclin G in NIH3T3 fibroblasts which express p53, as well as in 10.1 fibroblasts which contain no p53 allele. Additionally, we observed that while cyclin G expression is markedly reduced upon aggregate formation in embryonic carcinoma P19 cells, retrovirus-mediated over-expression of cyclin G enhances apoptotic cell death in aggregated P19 cells, and increases the extent of apoptosis caused by retinoic acid or serum starvation of these cells. These data demonstrate that cyclin G plays a facilitating role in modulating apoptosis induced by different stimuli. Moreover, we have discovered that cyclin G expression is rapidly induced in P19 cells after exposure to Bone Morphogenic Protein-4 (BMP-4), suggesting that cyclin G may mediate apoptotic signals generated by BMP-4.  (+info)

Interferon-alpha inhibits proliferation in human T lymphocytes by abrogation of interleukin 2-induced changes in cell cycle-regulatory proteins. (6/94)

IFN-alpha exerts prominent regulatory functions on the immune system. One such effect is the inhibition of proliferation of in vitro stimulated T lymphocytes. The exact physiological function of this activity is not known, but it has been implicated in the antiviral effects of IFN, its antitumor action in T-cell malignancies, and the regulation of the in vivo T-cell response. Here, we have investigated the mechanism underlying the IFN-alpha-mediated growth inhibition of normal human PHA- and IL-2-stimulated T lymphocytes by an analysis of how IFN-alpha treatment influences known molecular events that normally accompany the transition from quiescence to proliferation in these cells. IFN-alpha treatment was found to profoundly block S-phase entry of stimulated T lymphocytes. This correlated with a strong inhibition of IL-2-induced changes in G1-regulatory proteins, including the prevented up-regulation of G1 cyclins and cyclin-dependent kinases as well as an abrogation of mitogen-induced reduction of p27Kip1 levels. This latter effect was due to a maintained stability of the p27Kip1 protein in the IFN-alpha-treated cells. In line with these findings, phosphorylation of the pocket proteins was abrogated in IFN-alpha-treated cells. Furthermore, our data indicate that IFN-alpha has selective effects on the pathways that emerge from the IL-2 receptor because IFN-alpha treatment does not block IL-2-induced up-regulation of c-myc or Cdc25A.  (+info)

Role of cell cycle regulatory proteins in cerebellar granule neuron apoptosis. (7/94)

Cerebellar granule neurons (CGNs) undergo apoptosis when deprived of depolarizing concentrations of KCl, but the underlying molecular mechanisms are not yet clear. Although caspases have been postulated to be involved in CGN cell death, inhibitors of caspases failed to prevent apoptosis under our culture conditions, suggesting an involvement of other molecules and pathways. We find that inhibitors of cyclin-dependent kinases--flavopiridol, olomoucine, and roscovitine--protect CGNs from KCl withdrawal-induced apoptosis, suggesting that cell cycle components play a significant role in the death of these neurons. Analysis of the different cell cycle regulatory elements in this model revealed that apoptosis is preceded by an increase in the level of cyclin E protein, with elevated nuclear levels of cyclin D1 and with enhanced activity of the cyclin D1- and E- associated kinases. In addition, there was a significant decrease in the level of the cyclin-dependent kinase (cdk) inhibitor p27. In agreement with these changes, analysis of a major substrate of cyclin-activated cdks, retinoblastoma protein (Rb), showed an increase in the level of phosphorylated forms within 1 hr of KCl withdrawal. Moreover, the overall levels of Rb protein were significantly reduced within 6-12 hr of KCl withdrawal and did so by a caspase-independent mechanism. All of these responses were blocked by cdk inhibitors. These findings indicate that cdks act at an early step in the pathway by which KCl withdrawal induces apoptotic death of cerebellar granule cells and suggest that additional elements of the cell cycle machinery participate in this mechanism.  (+info)

Distinct roles for PP1 and PP2A in phosphorylation of the retinoblastoma protein. PP2a regulates the activities of G(1) cyclin-dependent kinases. (8/94)

The function of the retinoblastoma protein (pRB) in controlling the G(1) to S transition is regulated by phosphorylation and dephosphorylation on serine and threonine residues. While the roles of cyclin-dependent kinases in phosphorylating and inactivating pRB have been characterized in detail, the roles of protein phosphatases in regulating the G(1)/S transition are not as well understood. We used cell-permeable inhibitors of protein phosphatases 1 and 2A to assess the contributions of these phosphatases in regulating cyclin-dependent kinase activity and pRB phosphorylation. Treating asynchronously growing Balb/c 3T3 cells with PP2A-selective concentrations of either okadaic acid or calyculin A caused a time- and dose-dependent decrease in pRB phosphorylation. Okadaic acid and calyculin A had no effect on pRB phosphatase activity even though PP2A was completely inhibited. The decrease in pRB phosphorylation correlated with inhibitor-induced suppression of G(1) cyclin-dependent kinases including CDK2, CDK4, and CDK6. The inhibitors also caused decreases in the levels of cyclin D2 and cyclin E, and induction of the cyclin-dependent kinase inhibitors p21(Cip1) and p27(Kip1). The decrease in cyclin-dependent kinase activities were not dependent on induction of cyclin-dependent kinase inhibitors since CDK inhibition still occurred in the presence of actinomycin D or cycloheximide. In contrast, selective inhibition of protein phosphatase 1 with tautomycin inhibited pRB phosphatase activity and maintained pRB in a highly phosphorylated state. The results show that protein phosphatase 1 and protein phosphatase 2A, or 2A-like phosphatases, play distinct roles in regulating pRB function. Protein phosphatase 1 is associated with the direct dephosphorylation of pRB while protein phosphatase 2A is involved in pathways regulating G(1) cyclin-dependent kinase activity.  (+info)

The adaptive trial design of this advanced Phase II study incorporates (i) a dosing schedule based on the patients estimated tumor burden and not on standard dosing per kilogram body weight or body surface area, and (2) a tumor response evaluation process that is unique to the manner in which osteosarcoma responds favorably to therapy, i.e., with necrosis and increasing calcification in metastatic tumors and decreased glucose utilization using PET-CT imaging studies.. Twenty to thirty patients will receive Rexin-G at either Dose Level 1 or 2. Patients will be assigned a dose level based on the estimated tumor burden as measured by PET-CT imaging studies. Estimated tumor burden is measured by multiplying the sum of the longest diameters of target lesions in cm by 10e9 cancer cells. If the tumor burden is less than 10 billion cells, the patient will be assigned to Dose Level 1, if the tumor burden is greater than 10 billion cells, the patient will be assigned to Dose Level 2.. *Treatment Cycle ...
Cyclin G1, 0.1 mg. Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression.
Top performende anti-Schwein Cyclin G1 Antikörper für Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) vergleichen & kaufen.
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cyclin G associated kinase ENTREZID: 2580 | Type: NA | Map: 4p16.3 OMIM: 300335 Summary Entrez In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues.
SAN MARINO, Calif. and MANILA, Philippines - Nov. 6 (SEND2PRESS NEWSWIRE) -- Epeius Biotechnologies today announced the publication of clinical data from studies conducted at the University of the Philippines, Asian Hospital and Medical Center, Makati Medical Center, Manila, Philippines and Lutheran Medical Center, New York, USA, revealing the safety and single agent efficacy of Rexin-G(TM) for the treatment of a broad spectrum of chemotherapy-resistant cancers. - News from Epeius Biotechnologies Corporation, issued by Send2Press Newswire
The YDp plasmids (Yeast Disruption plasmids) are pUC9 vectors bearing a set of yeast gene disruption cassettes, all uniform in structure and differing only in the selectable marker used (HIS3, LEU2, LYS2, TRP1 or URA3). The markers, surrounded by translational termination codons, are embedded in the …
Summary of ANO7 (IPCA-5, NGEP, PCANAP5, PCANAP5L, TMEM16G) expression in human tissue. Cytoplasmic expression in prostate and stomach glands.
J:171486 Rutter M, Wang J, Huang Z, Kuliszewski M, Post M, Gli2 influences proliferation in the developing lung through regulation of cyclin expression. Am J Respir Cell Mol Biol. 2010 May;42(5):615-25 ...
This is in keeping with the theory that neuronal cell death associated with AD has, as its root cause, an ectopic re-entrance into the cell cycle (121), which results in the hyperphosphorylation of microtubule-associated tau proteins characteristic of AD neurofibrillary tangles. D1 and G1, and opposing tumor suppressor proteins, such as p53, pRb, p16INK4A and p21WAF1, which are commonly dysregulated in malignancy. While progress has been made in identifying several enzymes and molecular relationships associated with cell cycle checkpoint control, the designated complexity, particularly the functional redundancy, of these cell cycle control enzymes in mammalian systems, presents a major challenge CRT-0066101 in discerning an ideal locus for restorative treatment in the medical management of malignancy. Recent improvements in genetic engineering, practical genomics and medical oncology converged in identifying cyclin G1 (CCNG1 gene) like a pivotal component of a commanding cyclin G1/Mdm2/p53 axis ...
CCNG2 gene was initially identified in 1996 and encodes for a protein that belongs to a family of cyclins homologous to CCNG1 (7). Previous studies have reported that CCNG2 participates in carcinogenesis and is a known tumor suppressor gene (15-17,20-26). CCNG2 gene expression is downregulated in thyroid (20), oral (21), ovarian (22), breast (23,24), gastric (16), esophageal (17), prostate (25), kidney (26) and colorectal (15) cancer cells.. Several aspects of CCNG2 behavior are associated with antitumor effects. Antitumor agents induce CCNG2 expression, which results in the inhibition of cancer cell proliferation (8-10). In breast cancer, CCNG2 knockdown induces multidrug resistance (8). In colorectal cancer, CCNG2 expression correlates with the tumor stage, lymph node metastasis, clinical stage, histological grade and overall survival (15). In gastric cancer, CCNG2 expression correlates with the extent of differentiation: CCNG2 expression is high in well-differentiated adenocarcinomas and low ...
If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library, or send a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. ...
TGEN annouced that preliminary results from two Phase I trials of its adeno-associated virus vector bearing the CFTR gene show Read the full 203 word article
Untuk mengamankan file website dan database pada xampp gak harus menginstallnya di local disk D, menginstallnya di local disk C pun bisa
As of late, individuals are turning into increasingly health aware. Jadhav, U. & Jameson, J. L. Steroidogenic issue-1 (sf-1)-driven differentiation of murine embryonic stem (es) cells right into a gonadal lineage. Any steered medical therapies must be discussed with your physician. 1: Differentiation-induced pluripotent stem cells (iPSCs) into Leydig-like cells (iPSC-LCs) based on molecular compounds.. People on a ketogenic diet eat 50 grams or fewer of carbohydrates per day and as an alternative eat greater-than-regular amounts of fats and protein. Ge, R. S. & Hardy, M. P. Decreased cyclin a2 and increased cyclin g1 levels coincide with loss of proliferative capability in rat leydig cells throughout pubertal growth.. The main focus of public well being interventions is to forestall and manage ailments, accidents and different health conditions through surveillance of cases and the promotion of healthy conduct , communities , and (in points relevant to human well being) environments Its aim is ...
Speedy weight loss. With the help of right diet and a controlled diet, HGC allows you to lose weight at a much faster rate. HGC injections directly attack the unused fat deposits in your body and quickly burn it down in order to make you slim. Increased HGC level will primarily allow you to lose weight from arms, thighs, necks and other primary areas, making the whole process a lot faster.. Better metabolism. As HGC is responsible for controlling the metabolism of your body, the rise in its levels will definitely make your metabolism better. As HGC will start targeting the areas that are not often used by your body, you will get more energy which is directly related to increased metabolism.. Avoid hunger pains. A major difference between other diet and weight loss programs when compared to HGC plan is that there are no hunger pains. Despite the fact that your calorie intake will be cut down, it will be compensated by the fact that your body will extract that from unused fats that are stored in ...
Got blood test results back for the 3rd blood test I have had in about two weeks or so. First two where great with posotive reading & hormone level was good. Had to have 3rd blood test yesterday, got results back today. I cant work
Students choose 60 credits of modules: they do not have to take Dehonglir Gorffennol/Debating History but it does remain an option. Students must take at least one general module (code beginning HGH/HGC/HGW) over levels 5 and 6 as a whole ...
Remaja putri pelaku sex pranikah pasti bingung bin kelabakan kalo udah telat menstruasi, masalahnya selama ini telat menstruasi selalu identik dengan kehamilan, padahal belum tentu lho, apalagi kalo gak berhubungan sex sama sekali, masak gak ngapa-ngapain tiba-tiba hamil? (sumber: juraganmedis.com). Telat menstruasi atau bahasa kerennya amenorrhea sering terjadi pada banyak wanita, terutama pada remaja putri…
Bose P, Simmons GL, Grant S (2013). "Cyclin-dependent kinase inhibitor therapy for hematologic malignancies". Expert Opin ... Jain SK, Bharate SB, Vishwakarma RA (2012). "Cyclin-dependent kinase inhibition by flavoalkaloids". Mini Rev Med Chem. 12 (7): ...
Horne MC, Goolsby GL, Donaldson KL, Tran D, Neubauer M, Wahl AF (1996). "Cyclin G1 and cyclin G2 comprise a new family of ... CCNG1 cyclin G1". Zhao L, Samuels T, Winckler S, Korgaonkar C, Tompkins V, Horne MC, Quelle DE (Jan 2003). "Cyclin G1 has ... Cyclin-G1 is a protein that in humans is encoded by the CCNG1 gene. The eukaryotic cell cycle is governed by cyclin-dependent ... "Cyclin G1 overcomes radiation-induced G2 arrest and increases cell death through transcriptional activation of cyclin B1". Cell ...
G1/S-specific cyclin Cln3 is a protein that is encoded by the CLN3 gene. The Cln3 protein is a budding yeast G1 cyclin that ... This showed that the three G1 cyclins were responsible for controlling Start entry in budding yeast. The three G1 cyclins ... Although all three G1 cyclins are necessary for normal regulation of Start and the G1-S transition, Cln3 activity seems to be ... "Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins". The ...
It reduces the growth of new cells by inhibiting cyclin D1. As a result, cells arrest during G1 phase and enter apoptosis. ... "Endostatin causes G1 arrest of endothelial cells through inhibition of cyclin D1". J. Biol. Chem. 277 (19): 16464-9. doi: ...
For example, BCK2-null is synthetically lethal with G1 cyclin CLN3, and leads to a decrease in mRNA levels of another G1 cyclin ... A double deletion of BCK2 and G1 cyclin CLN3 results in inviability. or extremely slow growth. This was found to be a result of ... Di Como CJ, Chang H, Arndt KT (April 1995). "Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2". Molecular and ... The SBF and MBF complexes, which includes protein Swi6, regulate late G1 and G1/S transition genes. Overexpression of Bck2 in ...
Nature 332, 800-805 Deshaies, R.J., Chau, V., and Kirschner, M.W. (1995). Ubiquitination of the G1 cyclin Cln2p by a Cdc34p- ... Phosphorylation of Sic1p by G1 cyclin/Cdk is required for its degradation and entry into S phase. Science 278, 455-460 Seol, J. ... which he showed mediates conjugation of ubiquitin onto G1 cyclin proteins in yeast cells. Upon starting his laboratory at ... they established that an early step in the release of Cdc14 from Net1 is the phosphorylation of Net1 by the mitotic cyclin-Cdk ...
"Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1". Science. 284 (5414): 662-5. doi:10.1126 ... Maeda I, Ohta T, Koizumi H, Fukuda M (Apr 2001). "In vitro ubiquitination of cyclin D1 by ROC1-CUL1 and ROC1-CUL3". FEBS ...
Bose P, Simmons GL, Grant S (Jun 2013). "Cyclin-dependent kinase inhibitor therapy for hematologic malignancies". Expert ... CS1 maint: discouraged parameter (link) Martin MP, Olesen SH, Georg GI, Schönbrunn E (Nov 2013). "Cyclin-dependent kinase ... Fu W, Ma L, Chu B, Wang X, Bui MM, Gemmer J, Altiok S, Pledger WJ (Jun 2011). "The cyclin-dependent kinase inhibitor SCH 727965 ... Cyclin-dependent kinase inhibitor dinaciclib interacts with the acetyl-lysine recognition site of bromodomains. Dinaciclib ( ...
"CDK-Dependent Hsp70 Phosphorylation Controls G1 Cyclin Abundance and Cell-Cycle Progression". Cell. 151 (6): 1308-1318. doi: ... Subsequently, phosphorylation of chaperone protein HSP70 by a cyclin dependent kinase was shown to delay cell cycle progression ... in yeast and mammals by altering cyclin D1 stability (a key regulator of the cell cycle). Phosphorylation of HSP90 (another ...
December 2012). "CDK-dependent Hsp70 Phosphorylation controls G1 cyclin abundance and cell-cycle progression". Cell. 151 (6): ... Warrick JM, Chan HY, Gray-Board GL, Chai Y, Paulson HL, Bonini NM (December 1999). "Suppression of polyglutamine-mediated ...
The APC/CCdc20 does not recognize G1/S cyclins. Their concentration rises during G1, activating G1/S Cdks, which in turn ... The two main targets of the APC/C are the S/M cyclins and the protein securin. S/M cyclins activate cyclin-dependent kinases ( ... Multiple different mechanisms inhibit Cdks in G1: Cdk inhibitor proteins are expressed, and cyclin gene expression is down- ... It also targets S and M-phase (S/M) cyclins for destruction, which inactivates S/M cyclin-dependent kinases (Cdks) and allows ...
Xiao ZX, Ginsberg D, Ewen M, Livingston DM (1996). "Regulation of the retinoblastoma protein-related protein p107 by G1 cyclin- ... cyclins and cyclin dependent kinases". Oncogene. 15 (2): 143-57. doi:10.1038/sj.onc.1201252. PMID 9244350. Müller H, Moroni MC ...
"Estrogen and progesterone promote breast cancer cell proliferation by inducing cyclin G1 expression". Brazilian Journal of ... Blackburn GL, Wang KA (September 2007). "Dietary fat reduction and breast cancer outcome: results from the Women's Intervention ...
"Regulation of the retinoblastoma protein-related protein p107 by G1 cyclin-associated kinases". Proceedings of the National ... Retinoblastoma-like protein 1 has been shown to interact with: BEGAIN, BRCA1, BRF1, Cyclin A2, Cyclin-dependent kinase 2, E2F1 ... "Reversal of growth suppression by p107 via direct phosphorylation by cyclin D1/cyclin-dependent kinase 4". Molecular and ... Shanahan F, Seghezzi W, Parry D, Mahony D, Lees E (Feb 1999). "Cyclin E associates with BAF155 and BRG1, components of the ...
Sic1 releases SPF from inhibition after it is phosphorylated by G1-cyclin-CDK. The phosphorylation marking SPF for ... The S-phase-promoting factor (SPF) is a CDK-cyclin complex that induces the S-phase (synthesis phase) of the cell cycle. The S- ... Toward the end of the G1 phase of mitosis, an SPF is phosphorylated, causing a biochemical cascade that results in the ... a protein which also is a stoichiometric inhibitor of Cdk1-Clb and B-type cyclins). ...
Voit R, Hoffmann M, Grummt I (1999). "Phosphorylation by G1-specific cdk-cyclin complexes activates the nucleolar transcription ...
2002). "Endostatin causes G1 arrest of endothelial cells through inhibition of cyclin D1". J. Biol. Chem. 277 (19): 16464-9. ...
However, as Cyclin A accumulates and binds to Cdk2, they form a complex and inhibit p27. The G1 phase cyclin-dependent kinase ... yet the primary cyclin utilized is cyclin B. Cyclin B will serve as reference for discussion of the G2/M checkpoint transition ... During the beginning of the G1 phase, growth factors and DNA damage signal for the rise of cyclin D levels, which then binds to ... Skotheim JM, Di Talia S, Siggia ED, Cross FR (July 2008). "Positive feedback of G1 cyclins ensures coherent cell cycle entry". ...
Skotheim, Jan M.; Talia, Stefano Di; Siggia, Eric D.; Cross, Frederick R. (2008). "Positive feedback of G1 cyclins ensures ... "DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression". Nature ...
Also, the mitotic regulator for G1 progression, cyclin D1 was found to be activated. Although there was no increase in any ... It was indicated through Western blot that cyclin D1 was upregulated from day 1 to day 60 post-infection. It was also indicated ... through immunohistochemical analysis that the areas that produced the most cyclin D1 were the vasculature and interstitial ...
Through the repression of expression of cyclin E and cyclin A, Rb is able to inhibit the G1/S transition. There are at least ... Some genes activated during the G1/S transition such as cyclin E are repressed by HDAC during early to mid-G1 phase. This ... One such example of E2F-regulated genes repressed by Rb are cyclin E and cyclin A. Both of these cyclins are able to bind to ... When E2F is free it activates factors like cyclins (e.g. cyclin E and cyclin A), which push the cell through the cell cycle by ...
"The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases". Cell. 75 (4): 805-816. doi:10.1016/ ... Yue Xiong and David Beach as a cyclin-CDK-PCNA interacting protein (p21), and as a senescence derived inhibitor by Noda. ... "miR-6883 Family miRNAs Target CDK4/6 to Induce G1 Phase Cell-Cycle Arrest in Colon Cancer Cells". Cancer Research. 77 (24): ... "p21 is a universal inhibitor of cyclin kinases". Nature. 366 (6456): 701-704. Bibcode:1993Natur.366..701X. doi:10.1038/366701a0 ...
Cln3 (yeast): The Saccharomyces cerevisiae G1 cyclin Cln3, a primary regulator of cell cycle entry. This disambiguation page ...
Cyclin E dsRNA arrested the cell cycle at the G1 phase (before the S phase). Therefore, RNAi can target endogenous genes. In ... cyclin E dsRNA only diminished cyclin E RNA - a similar result was also shown using dsRNA corresponding to cyclin A which acts ... Cells transfected with cyclin E dsRNAs only showed degradation in cyclin E transcripts - the lacZ transcripts were stable. ... Sen GL, Blau HM (2005). "Argonaute2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies". Nature Cell ...
1993). "The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases". Cell. 75 (4): 805-16. doi: ... 2005). "Binding of HTm4 to cyclin-dependent kinase (Cdk)-associated phosphatase (KAP).Cdk2.cyclin A complex enhances the ... "The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases". Cell. 75 (4): 805-16. doi:10.1016/ ... "Chromosomal mapping of the genes for the human cell cycle proteins cyclin C (CCNC), cyclin E (CCNE), p21 (CDKN1) and KAP (CDKN3 ...
The activity of Cln1/2 is mediated by activation of a more upstream G1 cyclin, Cln3. Cln3, along with the cyclin-dependent ... G1/S genes include the cyclins Cln1 and Cln2, which can form active complexes with Cdk1. These activated Cln-Cdk complexes help ... proposed that the incomplete division was due to expression of genes in both the mating pathway and in the G1 cyclin-driven ... As mentioned above, the G1 cyclins, Cln1/2, are part of a positive feedback loop that promotes their own transcription and the ...
Cyclin dependent kinases (CDKs) are a group of several different kinases involved in regulation of the cell cycle. They ... Garrington, TP; Johnson, GL (Apr 1999). "Organization and regulation of mitogen-activated protein kinase signaling pathways". ... Lim, S.; Kaldis, P. (16 July 2013). "Cdks, cyclins and CKIs: roles beyond cell cycle regulation". Development. 140 (15): 3079- ... Different combinations of specific CDKs and cyclins mark different parts of the cell cycle. Additionally, the phosphorylation ...
1994). "p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest ... cyclin binding. • cyclin-dependent protein kinase activating kinase activity. • cyclin-dependent protein serine/threonine ... p21Cip1 (alternatively p21Waf1), also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a cyclin- ... CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, cyclin-dependent kinase inhibitor 1A, cyclin dependent kinase ...
Schwob, E; Böhm, T; Mendenhall, M. D.; Nasmyth, K (1994). "The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S ... "Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast". Cell. 81 (2): 269-278 ...
Li H, Okamoto K, Peart MJ, Prives C (February 2009). "Lysine-independent turnover of cyclin G1 can be stabilized by B'alpha ... This is achieved by continuous control of cyclins/CDKs levels through ubiquitination and degradation. When cyclin E is ... The level of cyclins, as the name suggests, are high only at certain time point during cell cycle. ... Witowsky JA, Johnson GL (January 2003). "Ubiquitylation of MEKK1 inhibits its phosphorylation of MKK1 and MKK4 and activation ...
NPAT activates histone gene expression only after it has been phosphorylated by the G1/S-Cdk cyclin E-Cdk2 in early S phase.[ ... NPAT is also a substrate of cyclin E-Cdk2, which is required for the transition between G1 phase and S phase. ... SLBP are marked for degradation by phosphorylation at two threonine residues by cyclin dependent kinases, possibly cyclin A/ ... This occurs when Whi5 is phosphorylated by Cdc8 which is a G1/S Cdk.[114] Suppression of histone gene expression outside of S ...
The cell cycle is divided into four distinct phases, G1, S, G2, and M. The G phases - which is the cell growth phase - makes up ... The cell cycle is regulated by a series of signalling factors and complexes such as cyclin-dependent kinases and p53, to name a ...
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. „Cell". 75 (4), s. 805-816, 1993. ... Regulation of cyclin A-Cdk2 by SCF component Skp1 and F-box protein Skp2. „Mol. Cell. Biol.". 19 (1), s. 635-45, 1999. PMID: ... Li Y, Jenkins CW, Nichols MA, Xiong Y. Cell cycle expression and p53 regulation of the cyclin-dependent kinase inhibitor p21. „ ... Watanabe H, Pan ZQ, Schreiber-Agus N, DePinho RA, Hurwitz J, Xiong Y. Suppression of cell transformation by the cyclin- ...
Cell cycle progression is controlled by ordered action of cyclin-dependent kinases (CDKs), activated by specific cyclins that ... Lambrou GI, Papadimitriou L, Chrousos GP, Vlahopoulos SA (April 2012). "Glucocorticoid and proteasome inhibitor impact on the ... After a CDK-cyclin complex has performed its function, the associated cyclin is polyubiquitinated and destroyed by the ... Mitotic cyclins, which persist in the cell for only a few minutes, have one of the shortest life spans of all intracellular ...
... the PDGFs allow a cell to skip the G1 checkpoints in order to divide.[21] It has been shown that in monocytes-macrophages and ...
G1/S transition of mitotic cell cycle. • negative regulation of cyclin-dependent protein serine/threonine kinase activity ... regulation of cyclin-dependent protein serine/threonine kinase activity. • negative regulation of G1/S transition of mitotic ... The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and ... cyclin-dependent protein serine/threonine kinase regulator activity. • protein binding. • ATP binding. • cyclin binding. • ...
Arif A, Jia J, Moodt RA, DiCorleto PE, Fox PL (January 2011). "Phosphorylation of glutamyl-prolyl tRNA synthetase by cyclin- ...
Cyclin. *Cyclin-dependent kinase inhibitor protein. *Cyclin-dependent kinase. *Cyclin. Lipid. *Phosphoinositide phospholipase C ...
... in the cyclin E-cdk2 complexes". Oncogene 20 (26): 3428-436. PMID 11423993. doi:10.1038/sj.onc.1204452.. ... Traído desde "https://gl.wikipedia.org/w/index.php?title=Licopeno&oldid=4861361" ... "Lycopene inhibition of cell cycle progression in breast and endometrial cancer cells is associated with reduction in cyclin D ...
All these phases in the cell cycle are highly regulated by cyclins, cyclin-dependent kinases, and other cell cycle proteins. ... Mitotic cells irradiated with X-rays in the G1 phase of the cell cycle repair recombinogenic DNA damages primarily by ...
regulation of cyclin-dependent protein serine/threonine kinase activity. • G1/S transition of mitotic cell cycle. • G2/M ... positive regulation of cyclin-dependent protein kinase activity. • negative regulation of G1/S transition of mitotic cell cycle ... cyclin-dependent protein kinase activating kinase activity. • cyclin binding. • ubiquitin protein ligase binding. • protein ... cyclin-dependent protein kinase holoenzyme complex. • nucleus. • nucleoplasm. • cytosol. • intracellular membrane-bounded ...
Dobrosotskaya IY, James GL (April 2000). "MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion ...
Codina J, Stengel D, Woo SL, Birnbaumer L (1986). "Beta-subunits of the human liver Gs/Gi signal-transducing proteins and those ... Cyclin. *Cyclin-dependent kinase inhibitor protein. *Cyclin-dependent kinase. *Cyclin. Lipid. *Phosphoinositide phospholipase C ... Buhl AM, Osawa S, Johnson GL (1995). "Mitogen-activated protein kinase activation requires two signal inputs from the human ... 1got: HETEROTRIMERIC COMPLEX OF A GT-ALPHA/GI-ALPHA CHIMERA AND THE GT-BETA-GAMMA SUBUNITS ...
It can be interpreted by counting, measuring, and analyzing the cells of the Sub/G1 cell population.[100] When HeLA cells are ... Cyclin. *A (A1, A2). *B (B1, B2, B3). *D (D1, D2, D3) ... Goldstein JC, Waterhouse NJ, Juin P, Evan GI, Green DR (March ... its activation coupled with G0/G1 phase cell cycle arrest" (PDF). Journal of Ethnopharmacology. 131 (3): 592-600. doi:10.1016/j ... p53 prevents the cell from replicating by stopping the cell cycle at G1, or interphase, to give the cell time to repair, ...
... has also been shown to be required for both G1-S phase progression and mitosis, and it also modulates the transcription ... Cyclin. *A (A1, A2). *B (B1, B2, B3). *D (D1, D2, D3) ...
Rakutsükkel M-mitoosifaas;raku jagunemine, G0-puhkefaas;rakk ei jagune, G1-valmistumine DNA sünteesiks,S-replikatsioonifaas, G2 ... Intracellular Control of Cell-Cycle Events: S-Phase Cyclin-Cdk Complexes (S-Cdks) Initiate DNA Replication Once Per Cycle. ... G1/S kontrollpunkt (restriktsiooni kontrollpunkt) reguleerib seda, kas eukarüootne rakk siseneb DNA replikatsiooni ja ...
ORF72 - vCyclin ORF73 - LANA, latency-associated nuclear antigen- tethers genome to chromosome during latency, also regulates ... ORF47 - gL - envelope glycoprotein involved in viral entry ORF49 - may be required for viral gene expression ... cyclin-D, a G protein-coupled receptor, interferon regulatory factor and Flice inhibitory protein (FLIP), as well as DNA ...
... cyclins and cyclin dependent kinases". Oncogene. 15 (2): 143-57. doi:10.1038/sj.onc.1201252. PMID 9244350.. ... negative regulation of G0 to G1 transition. • transcription from RNA polymerase II promoter. • signal transduction involved in ... "BRCA1 is phosphorylated at serine 1497 in vivo at a cyclin-dependent kinase 2 phosphorylation site". Mol. Cell. Biol. 19 (7): ...
Floyd SR, Porro EB, Slepnev VI, Ochoa GC, Tsai LH, De Camilli P (March 2001). "Amphiphysin 1 binds the cyclin-dependent kinase ... Leventis PA, Chow BM, Stewart BA, Iyengar B, Campos AR, Boulianne GL (November 2001). "Drosophila Amphiphysin is a post- ... Floyd SR, Porro EB, Slepnev VI, Ochoa GC, Tsai LH, De Camilli P (March 2001). "Amphiphysin 1 binds the cyclin-dependent kinase ...
Cyclin. *Cyclin-dependent kinase inhibitor protein. *Cyclin-dependent kinase. *Cyclin. Lipid. *Phosphoinositide phospholipase C ...
These transitions are controlled by the cyclin-dependent kinase Cdk1.[10] Though the proteins that control Cdk1 are well ... G1 phase), as well as increase in genetic material (G2 phase) following the replication during S phase.[1] This is not to be ... a cyclin-dependent kinase, on a tyrosine residue. Cdc2 drives entry into mitosis by phosphorylating a wide range of targets. ...
Klein RD, Sherman D, Ho WH, Stone D, Bennett GL, Moffat B, Vandlen R, Simmons L, Gu Q, Hongo JA, Devaux B, Poulsen K, Armanini ...
Cyclin. *Cyclin-dependent kinase inhibitor protein. *Cyclin-dependent kinase. *Cyclin. Lipid. *Phosphoinositide phospholipase C ...
negative regulation of cyclin-dependent protein serine/threonine kinase activity. • lung development. • cytokine-mediated ...
Bajaj M, Vadhera S, Brar AP, Soni GL (October 1997). "Role of oyster mushroom (Pleurotus florida) as hypocholesterolemic/ ... leading to an accumulation of cyclin-dependent kinase inhibitors p21 and p27, and to subsequent G1-phase arrest, as seen in ... "Lovastatin-mediated G1 arrest is through inhibition of the proteasome, independent of hydroxymethyl glutaryl-CoA reductase" ...
Cyclin D - Cdk 4/6 Mono-phosphorylates RbEdit. When a cell enters G1, Cyclin D- Cdk4/6 phosphorylates Rb at a single ... Some genes activated during the G1/S transition such as cyclin E are repressed by HDAC during early to mid-G1 phase. This ... Through the repression of expression of cyclin E and cyclin A, Rb is able to inhibit the G1/S transition. ... One such example of E2F-regulated genes repressed by Rb are cyclin E and cyclin A. Both of these cyclins are able to bind to ...
"Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1". Ann. N. Y. ... http://www.vivo.colostate.edu/hbooks/pathphys/endocrine/gi/secretin.html *↑ Osnes M, Hanssen LE, Flaten O, Myren J (1978). " ... Traído desde "https://gl.wikipedia.org/w/index.php?title=Secretina&oldid=4981645" ...
negative regulation of cyclin-dependent protein serine/threonine kinase activity. • G1/S transition of mitotic cell cycle. • ... CDKN2C, INK4C, p18, p18-INK4C, cyclin-dependent kinase inhibitor 2C, cyclin dependent kinase inhibitor 2C. ... CDKN2C‏ (Cyclin dependent kinase inhibitor 2C) هوَ بروتين يُشَفر بواسطة جين CDKN2C في الإنسان.[1][2][3] ... "Entrez Gene: CDKN2C cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)". مؤرشف من الأصل في 05 ديسمبر 2010.. الوسيط , ...
Most cyclists would like to cycle outside in summer and stay inside in winter. And a small number of cyclists would choose to cycle outside in freezing winter because they dont want to cut their exercise plan. If you are the one who has the determination to cycle in chilly winter, the most important thing for you is t
These observations show that cyclin levels can be rate-limiting for G1 progression in mammalian cells and suggest that cyclin ... a cyclin. Related kinases are also required for progression through the G1 phase in higher eukaryotes. The role of cyclins in ... Cyclin-dependent regulation of G1 in mammalian fibroblasts Message Subject. (Your Name) has forwarded a page to you from ... This was found to shorten the duration of G1, decrease cell size, and diminish the serum requirement for the transition from G1 ...
An essential G1 function for cyclin-like proteins in yeast.. Richardson HE1, Wittenberg C, Cross F, Reed SI. ... Cyclins were discovered in marine invertebrates based on their dramatic cell cycle periodicity. Recently, the products of three ... the essential function of Cln proteins was found to be limited to the G1 phase. Furthermore, the ability of the Cln proteins to ... shown to be essential for the G1 to S phase transition in S. cerevisiae. Because of the apparent functional redundancy of these ...
Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 ... The cyclin subunit imparts substrate specificity to the complex. Interacts with ATF5. Interacts with EIF3K. Component of the ... Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, ... Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) ...
Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 ... Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, ... Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) ... IPR013763. Cyclin-like. IPR036915. Cyclin-like_sf. IPR004367. Cyclin_C-dom. IPR015451. Cyclin_D. IPR006671. Cyclin_N. ...
However, we also report that Rho inhibits an alternative Rac/Cdc42-dependent pathway, which results in a strikingly early G1- ... The expression of cyclin D1 in mid-G1 phase is associated with sustained ERK activity, and we show here that Rho is required ... which results in a strikingly early G1-phase expression of cyclin D1. Thus, cyclin D1 is induced in mid-G1 phase because a Rho ... Timing of cyclin D1 expression within G1 phase is controlled by Rho Nat Cell Biol. 2001 Nov;3(11):950-7. doi: 10.1038/ncb1101- ...
... Nature. 2008 Jul 17;454(7202):291-6. doi: 10.1038/nature07118 ... positive feedback of the G1 cyclins Cln1 and Cln2 induces the near-simultaneous expression of the approximately 200-gene G1/S ... A similar G1/S regulatory network in mammalian cells, comprised of non-orthologous genes, suggests either conservation of ... incurring a large fluctuation-induced fitness penalty due to both the lack of cytoplasmic Cln2 and insufficient G1/S regulon ...
The cyclin-dependent kinase Cdk2 associates with cyclins A, D, and E and has been implicated in the control of the G1 to S ... cyclin E-Cdk2, cyclin D1-Cdk4, and cyclin D2-Cdk4 complexes. Cotransfection experiments indicate that CIP1 and SV40 T antigen ... CIP1 encodes a novel 21 kd protein that is found in cyclin A, cyclin D1, cyclin E, and Cdk2 immunoprecipitates. p21CIP1 is a ... The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.. Harper JW1, Adami GR, Wei N, ...
Cyclin E / metabolism. Cyclin-Dependent Kinase 2. Cyclin-Dependent Kinase 4. Cyclin-Dependent Kinase Inhibitor p16 / metabolism ... Cyclin-Dependent Kinases / antagonists & inhibitors*, metabolism. Cyclins / metabolism. Dose-Response Relationship, Drug. G1 ... 0/CDKN1A protein, human; 0/Cell Cycle Proteins; 0/Cyclin E; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase ... EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinase 4; EC 2.7.11.22/Cyclin-Dependent Kinases; EC 3.4. ...
The cyclin-dependent kinases 4 and 6 (Cdk4/6) that control the G1 phase of the cell cycle and their inhibitor, the p16INK4a ... The cyclin-dependent kinases 4 and 6 (Cdk4/6) that control the G1 phase of the cell cycle and their inhibitor, the p16INK4a ... MECHANISM OF G1 CYCLIN DEPENDENT KINASE INHIBITION FROM THE STRUCTURE OF THE CDK6-P16INK4A TUMOR SUPPRESSOR COMPLEX. *DOI: ... They prevent cyclin binding indirectly by causing structural changes that propagate to the cyclin-binding site. The INK4 ...
G1 cyclins Cln1-3; S phase B-type cyclins Clb5,6; and mitotic cyclins Clb1-4. These cyclins have sharply different biological ... 1996 The cyclin-dependent kinase inhibitor p40SIC1 imposes the requirement for Cln G1 cyclin function at Start. Proc. Natl. ... 1991 Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast. Cell 66: 1197-1206. ... 1991 An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins. Cell 66: 1207-1216. ...
Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the ... The G1 to S transition, however, appears to be controlled by the G1 cyclins. Cyclin D1 accumulates during G1 and associates ... amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 ... Cyclin E and Cdk2 interact during the G1 to S transition. Cyclin G contains a typical N terminal cyclin box and a carboxy ...
... cyclin G1 knockdown in HepG2 cells was obtained with two siRNAs (G1/238 and G1/832). Figure 2C shows a decrease in cyclin G1 ... A decrease of cyclin G1 mRNA was observed with both siRNAs. D, WB analysis of cyclin G1 and p53 expression in G1/832 siRNA- ... cyclin G1 siRNA (G1/832), or negative controls. Original magnification, ×10. B, cell cycle analysis of miR-122 and cyclin G1 ... MiR-122 and cyclin G1 modulate p53 expression. A, WB analysis of cyclin G1 and p53 levels in miR-122 transfected HepG2 cells. A ...
GFP vector and cyclin D3 (Cyclin D3), or PTEN and cyclin D3 (PTEN + Cyclin D3). Twenty-four h after transduction, the DNA ... 250 anti-cyclin D1 (Santa Cruz Biotechnology); 1:250 anti-cyclin D2 (Santa Cruz Biotechnology); or 1:250 anti-cyclin D3 (Santa ... Toyoshima H., Hunter T. p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21. Cell, 78: 67-74, ... Matsushime H., Roussel M. F., Ashmun R. A., Sherr C. J. Colony-stimulating factor 1 regulates novel cyclins during the G1 phase ...
Gene Set: REACTOME_CYCLIN_E_ASSOCIATED_EVENTS_DURING_G1_S_TRANSITION_. Standard name. REACTOME_CYCLIN_E_ASSOCIATED_EVENTS_ ...
... a G1 cyclin, positively regulates the expression of CLN1 and CLN2, two additional G1 cyclins whose expression during late G1 is ... Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2.. C J Di Como, H Chang, K T Arndt ... Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2. Message Subject (Your Name) has forwarded a page to you from ... In the absence of CLN3, bck2 mutations cause an extremely slow growth rate: the cells accumulate in late G1 with very low ...
Survey of the Human Pancreatic β-Cell G1/S Proteome Reveals a Potential Therapeutic Role for Cdk-6 and Cyclin D1 in Enhancing ... Survey of the Human Pancreatic β-Cell G1/S Proteome Reveals a Potential Therapeutic Role for Cdk-6 and Cyclin D1 in Enhancing ... Survey of the Human Pancreatic β-Cell G1/S Proteome Reveals a Potential Therapeutic Role for Cdk-6 and Cyclin D1 in Enhancing ... Survey of the Human Pancreatic β-Cell G1/S Proteome Reveals a Potential Therapeutic Role for Cdk-6 and Cyclin D1 in Enhancing ...
tr,A0A1J3JA08,A0A1J3JA08_NOCCA Cyclin-dependent kinase B1-2 (Fragment) OS=Noccaea caerulescens OX=107243 GN=MP_TR1527_c0_g1_i1_ ... Cyclin-dependent kinase B1-2Imported. ,p>Information which has been imported from another database using automatic procedures ... ORF Names:MP_TR1527_c0_g1_i1_g.3997Imported. ,p>Information which has been imported from another database using automatic ...
3B, cyclin D1 and cdk-6 alone were able to phosphorylate pRb as were the combinations of cyclin D1 plus cdk-4 or cyclin D1 plus ... Akt induces β-cell proliferation by regulating cyclin d1, cyclin d2, and p21 levels and cyclin-dependent kinase-4 activity. ... We find that in contrast to murine islets which contain little or no cdk-6 (17,23,35,36), each of the four G1/S cyclins and ... Survey of the Human Pancreatic β-Cell G1/S Proteome Reveals a Potential Therapeutic Role for Cdk-6 and Cyclin D1 in Enhancing ...
Effects of different carbon fluxes on G1 phase duration, cyclin expression, and reserve carbohydrate metabolism in ...
Cyclin G1 (CG1), an atypical cyclin, promoted G2-M arrest in PTCs and up-regulated TASCC formation. PTC TASCC formation was ... Cyclin G1 regulates G2-M arrest in proximal tubular cells, promoting a TASCC-induced secretory phenotype, fibrosis, and kidney ... Cyclin G1 regulates G2-M arrest in proximal tubular cells, promoting a TASCC-induced secretory phenotype, fibrosis, and kidney ... Cyclin G1 and TASCC regulate kidney epithelial cell G2-M arrest and fibrotic maladaptive repair ...
Cyclin E1 R ́ianti Cyclin E1 antibody j ́ACyclin E1 ^ p N ????o E T M , N [ i R ̂ł B c ... CDK2 ƕ ̂ ` āA זE G1/S ڍs 𒲐߂ T C N E ????oCyclin E1 R ... CCNE, CCNE1, cyclin E1, G1/S specific cyclin E1. GeneID. i ` q ... Cyclin E1 R ́ianti Cyclin E1 antibody j ́ACyclin E1 ^ p N ????o E T M , N [ i R ̂ł B ... Cyclin E1 Ƃ ?. T C N E1 icyclin E1 j ́ACCNE1 Ƃ Ă m ?? A x ɕۑ ꂽ T C N t @ ~ [ ɑ ^ p N ł B T C N t @ ~ [ ̃ o [ ́A זE ?ʂ ^ p N ʂ̋
G1 cyclins control the G1 to S phase transition in the budding yeast, Saccharomyces cerevisiae. Cyclin E was discovered in the ... Cyclin E-associated kinase activity was correlated with the appearance of complexes containing cyclin E and the cyclin- ... Thus, the cyclin E-Cdk2 complex may constitute a human G1-S phase-specific regulatory protein kinase. ... Association of human cyclin E with a periodic G1-S phase protein kinase ...
MEF-dependent transcription was suppressed by the expression of cyclin A but not by cyclin D or cyclin E. This effect was due ... Cyclin A-CDK2 phosphorylated MEF protein in vitro more efficiently than cyclin D-CDK4 or cyclin E-CDK2, and phosphorylation of ... Cyclin A / metabolism*. DNA Primers. DNA-Binding Proteins / chemistry, metabolism*, physiology. G1 Phase / physiology*. ... These amino acid substitutions also reduced the restriction of MEF activity to G1. Phosphorylation of MEF by the cyclin A-CDK2 ...
Cyclin D1 R ́ianti Cyclin D1 antibody j ́ACyclin D1 ^ p N ????o } E X m N [ i R ̂ł B c ... B cell lymphoma 1 protein, CCND1, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31. ... Cyclin D1 m N [ i R ́i i ԁF60186-1-Ig j. }1. Western blot of Cyclin D1 in HepG2, SW 1990 and NIH/3T3 cell lines with 60186-1-Ig ... זE G1/S ڍs CDK4/6 ƌ 钲 ߈ q A T C N D ????oCyclin D1 R ... Cyclin D1 R ́ianti Cyclin D1 antibody j ́ACyclin D1 ^ p N ????o ...
The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and ... Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenic and antimitogenic ... "Entrez Gene: CDK4 cyclin-dependent kinase 4". "CDK4 - Cyclin-dependent kinase 4 - Homo sapiens (Human) - CDK4 gene & protein". ... Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 ...
During G1 phase, the G1/S cyclin activity rises significantly near the end of the G1 phase. Complexes of cyclin that are active ... but not G1/S cyclins); and a high concentration of Cdk inhibitors is found during G1 phase. The restriction point (R) in the G1 ... At the G1/S checkpoint, formation of the G1/S cyclin with Cdk to form a complex commits the cell to a new division cycle. These ... The G1/S checkpoint is the point between G1 phase and the S phase in which the cell is cleared for progression into the S phase ...
  • Regulatory component of the cyclin D3- CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. (rcsb.org)
  • Cyclin D- CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. (rcsb.org)
  • Component of the ternary complex, cyclin D3/ CDK4 / CDKN1B , required for nuclear translocation and activity of the cyclin D- CDK4 complex. (rcsb.org)
  • p21CIP1 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk2, cyclin E-Cdk2, cyclin D1-Cdk4, and cyclin D2-Cdk4 complexes. (nih.gov)
  • The cyclin-dependent kinases 4 and 6 (Cdk4/6) that control the G1 phase of the cell cycle and their inhibitor, the p16INK4a tumour suppressor, have a central role in cell proliferation and in tumorigenesis. (rcsb.org)
  • The INK4 inhibitors also distort the kinase catalytic cleft and interfere with ATP binding, which explains how they can inhibit the preassembled Cdk4/6-cyclin D complexes as well. (rcsb.org)
  • Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5. (acris-antibodies.com)
  • Cyclin D associates with Cdk4/Cdk6 and the catalytic activities of the assembled holoenzymes are first manifested by mid-G 1 , increase to a maximum at the G 1 -S transition, and contribute to G 1 exit ( 5 - 8 ). (aacrjournals.org)
  • Cdk4/6-cyclin D, Cdk2-cyclin E, and the transcription complex that includes pRb and E2F are pivotal in controlling progression through the late G 1 restriction point ( 9 ). (aacrjournals.org)
  • Consistent with these findings, G 1 cyclin (D2 and D3) and CDK4 (cyclin-dependent kinase 4) levels and associated kinase activity were not affected. (portlandpress.com)
  • There was no apparent relationship, however, between cyclin D1 expression and the in vitro activity of its major kinase partner, Cdk4, although MDA-MB-134 cells displayed the highest level of both cyclin D1 expression and Cdk4 activity. (garvan.org.au)
  • These data suggest that properties of cyclins D1 and E in addition to their ability to activate Cdk4 and Cdk2 may contribute to the effects of overexpression on the breast cancer phenotype. (garvan.org.au)
  • CARI III inhibits the expression of cyclin D1, CDK4 and CDK2 and induces p21. (mdpi.com)
  • Cyclin-dependent kinase 4 also known as cell division protein kinase 4 is an enzyme that in humans is encoded by the CDK4 gene . (wikipedia.org)
  • CDK4 is a member of the cyclin-dependent kinase family. (wikipedia.org)
  • [5] Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. (wikipedia.org)
  • The expression of CDK4, cyclin D1, and phospho-Rb was markedly decreased in the A549-shTGIF cells compared with the A549-shcon cells, and p21 was markedly increased in the A549-shTGIF cells compared with the A549-shcon cells. (springer.com)
  • The G1 phase arrest is associated with down-regulation of Cyclin D1/Cdk4 and Cyclin B1/Cdc2 activities in cells after treatment with KG-135. (snu.ac.kr)
  • In addition, the decrease in the levels of Cyclin D1/Cdk4 and Cyclin B1, but not of Cdc2, is similarly prevented by co-treatment of cells with MG-132, a potent proteasome inhibitor. (snu.ac.kr)
  • Thus, the KG-135-induced arrest of the cell cycle at G1 phase in HeLa cells represents a novel mechanism that involves proteasome-mediated degradation of the Cyclins (Cyclin D1 and B 1) and Cdk4 proteins. (snu.ac.kr)
  • This article is from Oncology Letters, volume 7.AbstractPreclinical and clinical studies have demonstrated the anticancer activity of PD-0332991, a selective cyclin-dependent kinase 4-6 CDK4-6 inhibitor, in the treatment of various types of cancer in a retinoblastoma protein RB-dependent manner. (duhnnae.com)
  • Cdk4-cyclin E complexes form and initiate G1/Stransition. (mycareerpeer.com)
  • There was a concomitant inhibition of cyclin D1 expression and subsequent reduction in the formation of the cyclin D1-CDK4 complex. (aspetjournals.org)
  • Consistent with a decrease in the cyclin D1-CDK4 complex, NC381 treatment resulted in significant inhibition of pRb phosphorylation. (aspetjournals.org)
  • It is regulated by Cyclin D. Ribociclib are US FDA approved CDK4 and CDK6 inhibitors for the treatment of estrogen receptor positive/ HER2 negative advanced breast cancer. (wikipedia.org)
  • An essential G1 function for cyclin-like proteins in yeast. (nih.gov)
  • Using strains where CLN1 was expressed conditionally, the essential function of Cln proteins was found to be limited to the G1 phase. (nih.gov)
  • The data are consistent with the hypothesis that Cln proteins activate the Cdc28 protein kinase, shown to be essential for the G1 to S phase transition in S. cerevisiae. (nih.gov)
  • These findings demonstrate that lactoferrin induces growth arrest by modulating the expression and the activity of key G1 regulatory proteins. (biomedsearch.com)
  • OBJECTIVES To comprehensively inventory the proteins that control the G1/S cell cycle checkpoint in the human islet and compare them with those in the murine islet, to determine whether these might therapeutically enhance human β-cell replication, to determine whether human β-cell replication can be demonstrated in an in vivo model, and to enhance human β-cell function in vivo. (diabetesjournals.org)
  • RESEARCH DESIGN AND METHODS Thirty-four G1/S regulatory proteins were examined in human islets. (diabetesjournals.org)
  • Phosphorylation of MEF by the cyclin A-CDK2 complex controls its transcriptional activity during the cell cycle, establishing a novel link between the ETS family of proteins and the cell cycle machinery. (biomedsearch.com)
  • The Cdk2 then phosphorylates G1/S specific proteins, including proteins required for DNA replication initiation. (reactome.org)
  • Silencing the expression of cyclin G1 enhances the radiosensitivity of HCC cells in vitro and in vivo, and the mechanism is probably related to the regulation of apoptosis-related proteins. (researchsquare.com)
  • Cyclin is a family of proteins that control the progression of cells through the cell cycle by activating cyclin-dependent kinase (CDK) enzymes . (wikipedia.org)
  • For example, the amino-terminal regions of S and M cyclins contain short destruction-box motifs that target these proteins for proteolysis in mitosis. (wikipedia.org)
  • Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. (wikipedia.org)
  • This physical interaction is consistent with the genetic interaction between the CLN genes and CDC28 and suggests that Cln proteins are an essential component of the active protein kinase complex required for the G1 to S transition. (scripps.edu)
  • During G1 the cell grows, it increases its supply of proteins, organelles etc. (studystack.com)
  • The catalytic activity needed to modulate downstream events, but to be fully active these proteins need to combine with cyclins. (studystack.com)
  • They in turn promote the expression of *S cyclins* and proteins. (studystack.com)
  • The cyclin-dependent kinase Cdk2 associates with cyclins A, D, and E and has been implicated in the control of the G1 to S phase transition in mammals. (nih.gov)
  • CIP1 encodes a novel 21 kd protein that is found in cyclin A, cyclin D1, cyclin E, and Cdk2 immunoprecipitates. (nih.gov)
  • This G1 arrest is associated with a dramatic decrease in the protein levels of Cdk2 and cyclin E correlated with an inhibition of the Cdk2 kinase activity. (biomedsearch.com)
  • Cyclin E and Cdk2 interact during the G1 to S transition. (acris-antibodies.com)
  • However, PTEN induced a specific reduction of cyclin D3 levels and an associated increase in the amount of the inhibitor p27 KIP1 complexed with CDK2. (aacrjournals.org)
  • Cyclin E-associated kinase activity was correlated with the appearance of complexes containing cyclin E and the cyclin-dependent kinase Cdk2. (sciencemag.org)
  • Thus, the cyclin E-Cdk2 complex may constitute a human G1-S phase-specific regulatory protein kinase. (sciencemag.org)
  • Cdk2 associates with either cyclin E or cyclin A, and the resultant kinase activities increase at the G 1 -S transition or in the early S phase, respectively. (aacrjournals.org)
  • However, cyclin E-associated CDK2 activity, responsible for G 1 -to-S-phase progression, was inhibited. (portlandpress.com)
  • This decreased activity was accompanied by unchanged CDK2 protein levels and paradoxically elevated cyclin E and cyclin E-associated CDK2 levels, suggesting inhibition of the cyclin E-CDK2 complex. (portlandpress.com)
  • Androstenedione-induced G 1 exit correlated with increased cyclin E-associated kinase activity and increased CDK2 levels. (aacrjournals.org)
  • Letrozole treatment inhibited cyclin E-CDK2 kinase activity by preventing the androstenedione-induced increase in CDK2. (aacrjournals.org)
  • Similarly, there was no significant relationship between cyclin E expression and cyclin E-Cdk2 activity. (garvan.org.au)
  • 400 kDa, suggesting that formation of these complexes is a more important determinant of cyclin E-Cdk2 activity than cyclin E abundance. (garvan.org.au)
  • The transition from the G1 to S phase is controlled by the Cyclin E:Cdk2 complexes. (reactome.org)
  • As the Cyclin E:Cdk2 complexes are formed, the Cdk2 is phosphorylated by the Wee1 and Myt1 kinases. (reactome.org)
  • Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. (enquirebio.com)
  • mRNAs for cyclin E and Cdk2 have a role in the commitment to DNA replication in the cell cycle, and are induced in Rat-1A cells by serum stimulation. (elsevier.com)
  • Cyclin E and cdk2 genes are transcribed in quiescent cells, but their transcripts rapidly turn over and levels are kept low. (elsevier.com)
  • The rate of transcription of the cdk2 gene is slightly increased after serum stimulation, while that of cyclin E is fairly constant. (elsevier.com)
  • Artificial expression of an immediate-early protein ΔFosB results in proliferation of quiescent Rat-1A cells, and this is accompanied by an efficient induction of cyclin E and cdk2 mRNAs. (elsevier.com)
  • The expression of cyclin E and cdk2 genes is upregulated by stabilization of their transcripts, at least in part. (elsevier.com)
  • We propose that ΔFosB may have a role in regulation of progression of the cell cycle in serum-stimulated Rat-1A cells by triggering stabilization of mRNAs for cyclin E and Cdk2. (elsevier.com)
  • J , Nakabeppu, Y & Sekiguchi, M 1995, ' Stabilization of cyclin E and cdk2 mRNAs at G1/S transition in Rat-1A cells emerging from the G0 state ', Oncogene , vol. 10, no. 7, pp. 1343-1351. (elsevier.com)
  • Cyclin E2/CKD2 and cyclin E/CDK2 complexes phosphorylate histone H1 in vitro with similar specific activities and both are inhibited by p27(Kip1). (elsevier.com)
  • In cells, cyclin E2 is complexed with CDK2, p27 and p21. (elsevier.com)
  • Cyclin E, cyclin A and CDK2. (studystack.com)
  • In budding yeast, commitment occurs when the catalytic subunit of a protein kinase, encoded by the CDC28 gene (the homolog of the fission yeast cdc2+ gene), binds to a positively acting regulatory subunit, a cyclin. (sciencemag.org)
  • Consistent with the requirement for mitotic cyclin degradation for mitotic exit, precise genomic removal of the D box and KEN boxes from the budding yeast mitotic cyclin Clb2 caused a first-cycle block to mitotic exit ( Wäsch and Cross 2002 ). (genetics.org)
  • G1 cyclins control the G1 to S phase transition in the budding yeast, Saccharomyces cerevisiae. (sciencemag.org)
  • Cyclin E was discovered in the course of a screen for human complementary DNAs that rescue a deficiency of G1 cyclin function in budding yeast. (sciencemag.org)
  • In the budding yeast Saccharomyces cerevisiae the G(1) cyclins Cln1,2,3p control initiation of cell division. (mysciencework.com)
  • By such integration, we provide here a reliable transcriptional network at the G1-to-S transition in the budding yeast cell cycle. (biomedcentral.com)
  • Cell cycle progression in the budding yeast Saccharomyces cerevisiae is controlled by the Cdc28 protein kinase, which is sequentially activated by different sets of cyclins. (mysciencework.com)
  • The Whi3 protein is associated with the endoplasmic reticulum, interacts with Cdc28, the budding-yeast Cdk, binds the mRNA of cyclin CLN3 and prevents accumulation of the Cdc28-Cln3 in the nucleus until late G1. (udl.cat)
  • In budding yeast, G1 cyclins such as CLN1 and CLN2 are expressed in G1 and S phases, while mitotic cyclins such as CLB1 and CLB2 are expressed in G2 and M phases. (ox.ac.uk)
  • Related kinases are also required for progression through the G1 phase in higher eukaryotes. (sciencemag.org)
  • The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. (nih.gov)
  • Lactoferrin inhibits G1 cyclin-dependent kinases during growth arrest of human breast carcinoma cells. (biomedsearch.com)
  • THE eukaryotic cell cycle is regulated by cyclin-dependent kinases (CDKs) bound to cyclins ( Bloom and Cross 2007 ). (genetics.org)
  • Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression. (acris-antibodies.com)
  • P276-00, a flavone that inhibits cyclin-dependent kinases, has been identified by us recently as a novel antineoplastic agent. (aacrjournals.org)
  • This process is dependent on the activities of cyclin-dependent kinases (Cdk) that are sequentially regulated by cyclins D, E, and A ( 1 - 4 ). (aacrjournals.org)
  • The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. (lsbio.com)
  • G1/S-specific cyclin-D1 (CCND1) is a member of the Cyclin family which act as regulators of CDK kinases. (biosensis.com)
  • Cyclins, when bound with the dependent kinases , such as the p34 / cdc2 / cdk1 protein, form the maturation-promoting factor . (wikipedia.org)
  • Cyclins function as regulators of CDK kinases. (enquirebio.com)
  • Furthermore, down-regulation of G1 Cyclin-dependent kinase activities is kinetically well related to the decreased intracellular protein levels of these kinases. (snu.ac.kr)
  • Biochemical triggers known as cyclin-dependent kinases (Cdks) switch on cell cycles events at the corrected time and in the correct order to prevent any mistakes. (wikipedia.org)
  • Eukaryotic cells become committed to proliferate during the G1 phase of the cell cycle. (sciencemag.org)
  • The role of cyclins in controlling G1 progression in mammalian cells was tested by construction of fibroblasts that constitutively overexpress human cyclin E. This was found to shorten the duration of G1, decrease cell size, and diminish the serum requirement for the transition from G1 to S phase. (sciencemag.org)
  • The expression of cyclin D1 in mid-G1 phase is associated with sustained ERK activity, and we show here that Rho is required for the sustained ERK signal. (nih.gov)
  • However, we also report that Rho inhibits an alternative Rac/Cdc42-dependent pathway, which results in a strikingly early G1-phase expression of cyclin D1. (nih.gov)
  • Thus, cyclin D1 is induced in mid-G1 phase because a Rho switch couples its expression to sustained ERK activity rather than Rac and Cdc42. (nih.gov)
  • Our results show that Rho is crucial for maintaining the correct timing of cyclin D1 expression in G1 phase and describe a new role for cytoskeletal integrity in the regulation of cell cycle progression. (nih.gov)
  • The Cdc2- cyclin B complex controls the G2 to M transition whereas Cdc2-cyclin A regulates S phase progression. (acris-antibodies.com)
  • Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive. (acris-antibodies.com)
  • Cyclin A-dependent phosphorylation of the ETS-related protein, MEF, restricts its activity to the G1 phase of the cell cycle. (biomedsearch.com)
  • Using a green fluorescent protein gene reporter plasmid regulated by an MEF-responsive promoter, we determined that the transcriptional activity of MEF is largely restricted to the G1 phase of the cell cycle. (biomedsearch.com)
  • The G1 cyclins, cyclin D1 and E, are rate limiting for progression through G1 phase of the cell cycle in breast epithelial cells and are oncogenic when expressed in the mammary epithelium of transgenic mice. (garvan.org.au)
  • We have previously isolated two B-type cyclin genes, cycMs1 and cycMs2, from alfalfa that are primarily expressed during the G2-to-M phase transition and are most likely mitotic cyclin genes. (plantcell.org)
  • When differentiated G0-arrested cells were induced to reenter the cell cycle in the G1 phase and resume cell division by treatment with plant hormones, cycMs3 transcript levels increased long before the onset of DNA synthesis. (plantcell.org)
  • We propose that CycMs3 helps control reentry of quiescent G0-arrested cells into the G1 phase of the cell cycle. (plantcell.org)
  • The beginning of S-phase is marked by the first nucleotide being laid down on the primer during DNA replication at the early-firing origins.Failure to appropriately regulate cyclin E accumulation can lead to accelerated S phase entry, genetic instability, and tumorigenesis. (reactome.org)
  • In the model yeast Saccharomyces cerevisiae , the commitment to a new round of cell division takes place towards the end of the G1 phase of the cell cycle, a process called START [ 1 ]. (biomedcentral.com)
  • Negative regulation of G1 and G2 by S-phase cyclins of Sacchar. (mysciencework.com)
  • The consequences of this negative regulation were most apparent in clb6 mutants, which had a shorter pre-Start G1 phase as well as a shorter G2 phase than congenic wild-type cells. (mysciencework.com)
  • It was more difficult to assess the role of Clb5 in G1 and G2 by genetic analysis because of the extreme prolongation of S phase in clb5 mutants. (mysciencework.com)
  • We show that ectopic expression of FBXO31 acts through a proteasome-directed pathway to mediate the degradation of cyclin D1, an important regulator of progression from G1 to S phase, resulting in arrest in G1. (umassmed.edu)
  • G1/S Cyclins rise in late G1 and fall in early S phase. (wikipedia.org)
  • The Cdk- G1/S cyclin complex begins to induce the initial processes of DNA replication, primarily by arresting systems that prevent S phase Cdk activity in G1. (wikipedia.org)
  • The levels of S cyclins remain high, not only throughout S phase, but through G2 and early mitosis as well to promote early events in mitosis. (wikipedia.org)
  • [6] Expression of cyclins detected immunocytochemically in individual cells in relation to cellular DNA content (cell cycle phase), [7] or in relation to initiation and termination of DNA replication during S-phase, can be measured by flow cytometry . (wikipedia.org)
  • Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. (enquirebio.com)
  • enQuire Bio's product, Cyclin E (G1/S-Phase Cyclin) MonoSpecific Antibody, is available for Research Use Only (RUO-Only). (enquirebio.com)
  • Genetic analysis has revealed that G1-phase cyclins are involved in the relationship in terms of cell size and commitment to cell division. (asj-oa.am)
  • To identify the genetic pathways that may link G1-phase cyclins to cell size, we conducted a systematic genomic- wide genetic screen in yeast. (asj-oa.am)
  • The antiproliferative effects of TEA, FSK, DFSK, and veratridine were attributable to OP cell cycle arrest in G1 phase. (jneurosci.org)
  • It has been hypothesized that voltage-dependent K + channel activity could regulate mitogenesis in the nervous system by maintaining the membrane potential hyperpolarized, a condition necessary for progression through G1 phase restriction points ( Wonderlin and Strobl, 1996 ). (jneurosci.org)
  • and (2) cyclins and cyclin-dependent kinase inhibitors (cdkis) known to regulate cell cycle progression through this phase are affected by ion channel activity or changes in membrane potential. (jneurosci.org)
  • It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (wikipedia.org)
  • The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16 INK4a . (wikipedia.org)
  • Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. (wikipedia.org)
  • Hypophosphorylates RB1 in early G1 phase. (wikipedia.org)
  • The silencing of TGIF inhibited A549 cell proliferation, colony formation in vitro, growth of tumor xenograft in vivo, and arrested the cell cycle in the G1 phase. (springer.com)
  • Cell cycle synchronization clearly showed coexpression of cyclin G1 and cyclin B1 in G2/M phase. (elsevier.com)
  • KG-135 arrests cells at the G1 phase of the cell cycle with an IC(50) value of 69 mu g/ml. (snu.ac.kr)
  • In two malignantly transformed cell lines, cyclin E2 activity is sustained throughout S phase, while cyclin E activity has already declined and cyclin A activity is only beginning to rise. (elsevier.com)
  • Further, a transcriptional analysis (RNA-seq) revealed that THTMP targeted the p53 signaling pathway specific genes causing DNA damage and cell cycle arrest at G1/S phase explained by the decrease of cyclin-dependent kinase 1, cyclin A2, cyclin E1 and E2 in glioma cells. (tut.fi)
  • Human cyclin A2 is a key regulator of S phase progression and entry into mitosis. (duhnnae.com)
  • The S. cerevisiae CLN genes encode cyclin homologs essential for progression from G1 to S phase. (scripps.edu)
  • The abundance of the CLN1 and CLN2 transcripts increases greater than 5-fold during the G1 interval, decreasing dramatically as cells enter S phase. (scripps.edu)
  • 6-Gingerol induces cell-cycle G1-phase arrest through AKT-GSK 3β-cyclin D1 pathway in renal-cell carcinoma. (greenmedinfo.com)
  • G1, S and G2 phase. (studystack.com)
  • When does the G1 phase occur and what is it? (studystack.com)
  • The G1 (Gap 1) phase is the phase following mitosis. (studystack.com)
  • G0 phase is the 'quiescent' or resting phase, the cell enters this phase from G1 and may do so temporarily or permanently. (studystack.com)
  • In G1, during replication (S-phase) and after replication (in G2). (studystack.com)
  • The g1 phase, gap 1 phase, or growth 1 phase, is the first of four phases of the cell cycle that takes place in eukaryotic cell division. (wikipedia.org)
  • G1 phase ends when the cell moves into the S phase of interphase. (wikipedia.org)
  • G1 phase together with the S phase and G2 phase comprise the long growth period of the cell cycle cell division called interphase that takes place before cell division in mitosis (M phase). (wikipedia.org)
  • During G1 phase, the cell grows in size and synthesizes mRNA and protein that are required for DNA synthesis. (wikipedia.org)
  • The duration of each phase, including the G1 phase, is different in many different types of cells. (wikipedia.org)
  • In human somatic cells, the cell cycle lasts about 18 hours, and the G1 phase takes up about 1/3 of that time. (wikipedia.org)
  • However, in Xenopus embryos, sea urchin embryos, and Drosophila embryos, the G1 phase is barely existent and is defined as the gap, if one exists, between the end of mitosis and the S phase. (wikipedia.org)
  • G1 phase and the other subphases of the cell cycle may be affected by limiting growth factors such as nutrient supply, temperature, and room for growth. (wikipedia.org)
  • In humans, the normal physiological temperature is around 37 °C (98.6 °F). G1 phase is particularly important in the cell cycle because it determines whether a cell commits to division or to leaving the cell cycle. (wikipedia.org)
  • If a cell is signaled to remain undivided, instead of moving onto the S phase, it will leave the G1 phase and move into a state of dormancy called the G0 phase. (wikipedia.org)
  • During G1 phase, the G1/S cyclin activity rises significantly near the end of the G1 phase. (wikipedia.org)
  • and a high concentration of Cdk inhibitors is found during G1 phase. (wikipedia.org)
  • The restriction point (R) in the G1 phase is different from a checkpoint because it does not determine whether cell conditions are ideal to move on to the next phase, but it changes the course of the cell. (wikipedia.org)
  • After a vertebrate cell has been in the G1 phase for about three hours, the cell enters a restriction point in which it is decided whether the cell will move forward with the G1 phase or move into the dormant G0 phase. (wikipedia.org)
  • Between the beginning of the G1 phase (which is also after mitosis has occurred) and R, the cell is known as being in the G1-pm subphase, or the post-mitotic phase. (wikipedia.org)
  • After R and before S, the cell is known as being in G1-ps, or the pre S phase interval of the G1 phase. (wikipedia.org)
  • In order for the cell to continue through the G1-pm, there must be a high amount of growth factors and a steady rate of protein synthesis, otherwise the cell will move into G0 phase. (wikipedia.org)
  • Some authors will say that the restriction point and the G1/S checkpoint are one and the same, but more recent studies have argued that there are two different points in the G1 phase that check the progression of the cell. (wikipedia.org)
  • The G1/S checkpoint is the point between G1 phase and the S phase in which the cell is cleared for progression into the S phase. (wikipedia.org)
  • These observations show that cyclin levels can be rate-limiting for G1 progression in mammalian cells and suggest that cyclin synthesis may be the target of physiological signals that control cell proliferation. (sciencemag.org)
  • Recently, the products of three genes associated with cell cycle progression in S. cerevisiae were found to share limited homology with cyclins. (nih.gov)
  • The ability of mitotic B-type cyclins to both induce mitotic entry and block mitotic exit may tightly couple many aspects of cell cycle progression to once-per-CDK-cycle ( Nasmyth 1996 ). (genetics.org)
  • Cdk-6 and cyclin D 1 in vitro led to marked activation of retinoblastoma protein phosphorylation and cell cycle progression with no induction of cell death. (diabetesjournals.org)
  • G1 cyclins (D-type cyclins and E-type cyclins) promote G1/S progression and are aberrantly expressed in cancer . (dartmouth.edu)
  • TFIID comprises the TATA box-binding protein and a set of highly conserved associated factors (TAF(II)s). yTAF(II)145, the core subunit of the yeast TAF(II) complex, is dispensable for transcription of most yeast genes but specifically required for progression through G1/S. Here we show that transcription of G1 and certain B-type cyclin genes is dependent upon yTAF(II)145. (umassmed.edu)
  • While it is now well established that inhibition of cyclin/CDK complexes by p21 can result in G1 cell cycle arrest, the consequences of p21/PCNA interaction on cell cycle progression have not yet been determined. (mendeley.com)
  • Our results suggest that p21 might inhibit cell cycle progression by two independent mechanisms, inhibition of cyclin/CDK complexes, and inhibition of PCNA function resulting in both G1 and G2 arrest. (mendeley.com)
  • Alternative splice variants of the G1 and mitotic cyclins have been shown to interfere with full-length cyclin functions to modulate cell cycle progression and are therefore likely to play a role in differentiation or oncogenesis. (duhnnae.com)
  • The structures of Cdk6 bound to p16INK4a and to the related p19INK4d reveal that the INK4 inhibitors bind next to the ATP-binding site of the catalytic cleft, opposite where the activating cyclin subunit binds. (rcsb.org)
  • Fractionation of whole cell lysates by gel filtration chromatography revealed that approximately 90% of the cyclin E protein was present in inactive complexes containing the CDK inhibitors p21 and p27. (garvan.org.au)
  • Cyclin-Dependent Kinase Inhibitors as Marketed Anticancer Drugs: Where Are We Now? (mdpi.com)
  • A unique feature of p21 that distinguishes it from the other cyclin-dependent kinase (CDK) inhibitors is its ability to associate with the proliferating cell nuclear antigen (PCNA), an auxiliary factor for DNA polymerases delta and epsilon. (mendeley.com)
  • Blockage of K + channels and membrane depolarization also caused accumulation of the cyclin-dependent kinase inhibitors p27 Kip1 and p21 CIP1 in OP cells. (jneurosci.org)
  • The antiproliferative effects of K + channel blockers and veratridine were still present in OP cells isolated from INK4a −/− mice, lacking the cyclin-dependent kinase inhibitors p16 INK4a and p19 ARF . (jneurosci.org)
  • Like cyclin E, cyclin E2 is an unstable protein in vivo and is stabilized by proteasome inhibitors. (elsevier.com)
  • Cells with the CLN1 and CLN2 genes deleted frequently arrest as unbudded cells, incurring a large fluctuation-induced fitness penalty due to both the lack of cytoplasmic Cln2 and insufficient G1/S regulon expression. (nih.gov)
  • In this in vitro study, we demonstrate that treatment of breast carcinoma cells MDA-MB-231 with human lactoferrin induces growth arrest at the G1 to S transition of the cell cycle. (biomedsearch.com)
  • CLB3 ∆ db cells show no cell cycle arrest response to mating pheromone, and CLB3 ∆ db completely bypasses the requirement for CLN G 1 cyclins, even in the absence of the early expressed B-type cyclins CLB5 , 6 . (genetics.org)
  • Enforced expression of cyclin D3 abrogated the PTEN-induced cell cycle arrest. (aacrjournals.org)
  • Overexpression of low-molecular-weight (LMW) cyclin E can bypass this process and renders letrozole ineffective in mediating growth arrest. (aacrjournals.org)
  • Here, we report the isolation of a novel alfalfa cyclin gene, termed cycMs3 (for cyclin Medicago sativa), by selecting for mating type alpha-pheromone-induced cell cycle arrest suppression in yeast. (plantcell.org)
  • Detailed analysis of the molecular mechanism of ATRA-induced cell cycle arrest and differentiation showed that the onset of cell cycle arrest was associated with a decrease in c-Myc and cyclin E levels and upregulation of p27 Kip1 and p21 WAF1/CIP1 . (diva-portal.org)
  • The inhibition of ATRA-induced cell-cycle arrest by constitutive expression of Stat1Y701F or Stat1S727A was associated with impaired regulation of these cyclins and p27 Kip1 , positioning Stat1 activation upstream of these events. (diva-portal.org)
  • RNAi-mediated knockdown of FBXO31 prevents cells from undergoing efficient arrest in G1 after gamma-irradiation and markedly increases sensitivity to DNA damage. (umassmed.edu)
  • Our results reveal FBXO31 as a regulator of the G1/S transition that is specifically required for DNA damage-induced growth arrest. (umassmed.edu)
  • CARI III demonstrates anti-proliferative activity against B16F10 melanoma cells through inducing G0/G1 cell cycle arrest. (mdpi.com)
  • Here, we show, using a tetracycline-regulated system, that expression of wild-type p21 in p53-deficient DLD1 human colon cancer cells inhibits DNA synthesis and causes G1 and G2 cell cycle arrest. (mendeley.com)
  • Our results demonstrate that blockage of K + channels and cell depolarization induce G1 arrest in the OP cell cycle through a mechanism that may involve p27 Kip1 and p21 CIP1 and further support the conclusion that OP cell cycle arrest and differentiation are two uncoupled events. (jneurosci.org)
  • Therefore, our data suggest that cyclin G1 enhanced radiation sensitivity by overriding radiation-induced G2 arrest through transcriptional upregulation of cyclin B1. (elsevier.com)
  • THTMP treatment led to G1/S cell cycle arrest and apoptosis induction of glioma cell lines. (tut.fi)
  • We show that A2V6 induced a clear G1-S cell cycle arrest associated with a p21 and p27 upregulation and an inhibition of retinoblastoma protein phosphorylation. (duhnnae.com)
  • Vol 7: PD-0332991 induces G1 arrest of colorectal carcinoma cells through inhibition of the cyclin-dependent kinase-6 and retinoblastoma protein axis. (duhnnae.com)
  • PD-0332991 treatment blocked RB phosphorylation and inhibited cell growth through the induction of G1 arrest of colorectal carcinoma cells. (duhnnae.com)
  • In contrast to current models proposing a linear cascade of Start activation, transcriptional positive feedback of the G1 cyclins Cln1 and Cln2 induces the near-simultaneous expression of the approximately 200-gene G1/S regulon. (nih.gov)
  • Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2. (asm.org)
  • The Saccharomyces cerevisiae CLN3 protein, a G1 cyclin, positively regulates the expression of CLN1 and CLN2, two additional G1 cyclins whose expression during late G1 is activated, in part, by the transcription factors SWI4 and SWI6. (asm.org)
  • In the absence of CLN3, bck2 mutations cause an extremely slow growth rate: the cells accumulate in late G1 with very low levels of CLN1 and CLN2 RNA. (asm.org)
  • The results suggest that BCK2 and CLN3 provide parallel activation pathways for the expression of CLN1 and CLN2 during late G1. (asm.org)
  • They also require the CDC28 protein kinase and at least one of three G1-specific cyclins encoded by CLN1, 2, and 3. (ox.ac.uk)
  • Cell changes in the cell cycle like the assembly of mitotic spindles and alignment of sister-chromatids along the spindles are induced by M cyclin- Cdk complexes. (wikipedia.org)
  • Complexes of cyclin that are active during other phases of the cell cycle are kept inactivated to prevent any cell-cycle events from occurring out of order. (wikipedia.org)
  • abstract = "Although cyclin G1 has been implicated in certain p53-related biological phenomena, other aspects of its function remain unclear. (elsevier.com)
  • The G1 to S transition, however, appears to be controlled by the G1 cyclins. (acris-antibodies.com)
  • cycMs3, a novel B-type alfalfa cyclin gene, is induced in the G0-to-G1 transition of the cell cycle. (plantcell.org)
  • The G1-to-S transition of the cell cycle in the yeast Saccharomyces cerevisiae involves an extensive transcriptional program driven by transcription factors SBF (Swi4-Swi6) and MBF (Mbp1-Swi6). (biomedcentral.com)
  • 60% of TNBCs, drives a mitogen-independent G1/S cell cycle transition through cytoplasm localization. (nature.com)
  • CCND1 is essential for the control of the cell cycle at the G1/S (start) transition. (biosensis.com)
  • At the G1/S transition of serum-stimulated cells, transient stabilization of the two types of mRNAs occurs, an event which may lead to induction of each mRNA. (elsevier.com)
  • The slow growth rate and long G1 delay of bck2 cln3 mutants are cured by heterologous expression of CLN2. (asm.org)
  • Nevertheless, both Clb5 and Clb6 were shown to be responsible for down-regulation of the protein kinase activities associated with Cln2, a G1 cyclin, and Clb2, a mitotic cyclin, in vivo. (mysciencework.com)
  • The CLN2 gene encodes a 62 kd polypeptide that accumulates periodically, peaking during G1 and decreasing rapidly thereafter, and is rapidly lost following exposure of cells to mating pheromone. (scripps.edu)
  • Cln2 abundance can be explained by the G1-specific accumulation of the CLN2 transcript coupled with instability of the Cln2 protein. (scripps.edu)
  • The effect of overexpression of cdk-4, cdk-6, and cyclin D 1 on phosphorylation of the retinoblastoma protein, pRb. (diabetesjournals.org)
  • Cdk-6 overexpression, alone or in combination with cyclin D 1 , strikingly stimulates human β-cell replication, both in vitro as well as in vivo, without inducing cell death or loss of function. (diabetesjournals.org)
  • Most importantly, we show that combined overexpression of cdk-6 with cyclin D 1 also leads to human β-cell replication in vivo and results in enhanced human islet engraftment and function in an in vivo transplant diabetes model. (diabetesjournals.org)
  • In order to investigate the functional correlates of cyclin D1 and cyclin E overexpression we employed a panel of normal, immortalized and neoplastic breast epithelial cell lines to examine the relationships between cyclin gene expression, cyclin-CDK complex formation and CDK activity. (garvan.org.au)
  • Overexpression of cyclin E promoted anchorage-independent growth of mouse lung epithelial cells . (dartmouth.edu)
  • Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. (enquirebio.com)
  • Overexpression of cyclin G1 was observable in lung carcinoma tissues. (elsevier.com)
  • Irradiation of human lung cells with cyclin G1 overexpression resulted in increased cell death and γ-H2AX foci suggesting that cyclin G1 rendered the cells more susceptible to DNA damage. (elsevier.com)
  • A similar G1/S regulatory network in mammalian cells, comprised of non-orthologous genes, suggests either conservation of regulatory architecture or convergent evolution. (nih.gov)
  • On the other hand, deletion of many cyclin genes leads to, at most, minor defects. (genetics.org)
  • The yeast Saccharomyces cerevisiae has three G1 cyclin (CLN) genes with overlapping functions. (asm.org)
  • Yeast TAF(II)145 required for transcription of G1/S cyclin genes and r" by Scott S. Walker, Wu-Cheng Shen et al. (umassmed.edu)
  • Cyclins themselves have no enzymatic activity but have binding sites for some substrates and target the Cdks to specific subcellular locations. (wikipedia.org)
  • Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. (acris-antibodies.com)
  • Transduction of human islets with Ad.cdk-6, Ad.cyclin D 1 , or combinations of Ad.cdk-4 plus Ad.cyclin D 1 or Ad.cdk-6 plus Ad.cyclin D 1 leads to phosphorylation of endogenous pRb. (diabetesjournals.org)
  • mutation of three serine or threonine residues in this region significantly reduced phosphorylation of MEF by cyclin A and reduced cyclin A-mediated suppression of its transactivating activity. (biomedsearch.com)
  • Cyclin D1 degradation results from a direct interaction with FBXO31 and is dependent on the F-box motif of FBXO31 and phosphorylation of cyclin D1 at Thr 286, which is known to be required for cyclin D1 proteolysis. (umassmed.edu)
  • A cyclin forms a complex with Cdk, which begins to activate but the complete activation requires phosphorylation, as well. (wikipedia.org)
  • Cyclin G1 antibody LS-C415844 is an unconjugated rabbit polyclonal antibody to Cyclin G1 (CCNG1) from human, mouse and rat. (lsbio.com)
  • Cyclin G1 antibody LS-C481195 is an HRP-conjugated rabbit polyclonal antibody to Cyclin G1 (CCNG1) from human, bat, bovine and other species. (lsbio.com)
  • CCNG1 / Cyclin G antibody Western blot of MCF7 Cell lysate. (lsbio.com)
  • CCNG1 (Cyclin G1) is a Protein Coding gene. (genecards.org)
  • The C9orf72 deficiency also induced ccng1 (encodes Cyclin G1) mRNA expression, and injection of a dominant-negative Cyclin G1 construct rescued the axonal impairment, apoptosis, and motility defects in the C9orf72-deficient embryos. (elsevier.com)
  • upon B-type cyclin degradation, no further mitotic entry events occur, but mitotic exit is allowed ( Nasmyth 1996 ). (genetics.org)
  • Mice constitutively overexpressing wild-type or degradation-resistant mutant cyclin E in the lung under the control of human surfactant protein C (SP-C) promoter developed respiratory epithelium premaligancy and malignancy and showed evidence of local metastasis . (dartmouth.edu)
  • F-box protein FBXO31 mediates cyclin D1 degradation to induce G1 arres" by Manas K. Santra, Narendra Wajapeyee et al. (umassmed.edu)
  • Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (enquirebio.com)
  • The anaphase-promoting complex (APC) which promotes the degradation of mitotic cyclins. (studystack.com)
  • Thus, the in vitro cellular potency, together with in vivo antitumor activity, confirms the potential of P276-00, a cyclin-dependent kinase inhibitor as an anticancer molecule. (aacrjournals.org)
  • The antiproliferative effects of the AIs were examined in aromatase-overexpressing MCF-7/Ac1 cells in the presence or absence of full-length cyclin E and LMW-E. Inhibition of LMW cyclin E kinase activity by roscovitine [a cyclin-dependent kinase (CDK) inhibitor] was examined in letrozole-unresponsive MCF-7/Ac1 cells. (aacrjournals.org)
  • Inhibition of cyclin E/cyclin-dependent kinase 2 activity through increased binding of the cell cycle inhibitor p27 to the complex is a key mediator of the antiproliferative effects of letrozole. (aacrjournals.org)
  • Lastly, we show that breast cancer patients whose tumors overexpress LMW cyclin E are more likely to recur after AI treatment compared with those with low or no expression of LMW cyclin E. These data support clinical investigation of cyclin-dependent kinase 2 inhibitor therapy for postmenopausal women with estrogen receptor-positive, LMW cyclin E-expressing breast cancer. (aacrjournals.org)
  • We have previously shown that miR-122 can regulate the expression of cyclin G1, whose high levels have been reported in several human cancers. (aacrjournals.org)
  • In the present study, we show, in addition, that these cyclins negatively regulate G1- and G2-specific functions. (mysciencework.com)
  • We speculate that cyclin E2 is not simply redundant with cyclin E, but may regulate distinct rate-limiting pathway(s) in G1-S control. (elsevier.com)
  • In Saccharomyces cerevisiae , four B-type cyclins, Clb1 - 4 , carry out essential mitotic roles, with substantial but incomplete overlap of function among them. (genetics.org)
  • The G1 cyclin Cln3p controls vacuolar biogenesis in Saccharomyces cerevisiae. (mysciencework.com)
  • We proved that, by modulating cyclin G1, miR-122 influences p53 protein stability and transcriptional activity and reduces invasion capability of HCC-derived cell lines. (aacrjournals.org)
  • Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency. (acris-antibodies.com)
  • We evaluated the role of miR-122 and cyclin G1 expression in hepatocarcinogenesis and in response to treatment with doxorubicin and their relevance on survival and time to recurrence (TTR) of HCC patients. (aacrjournals.org)
  • In addition, in a therapeutic perspective, we assayed the effects of a restored miR-122 expression in triggering doxorubicin-induced apoptosis and we proved that miR-122, as well as cyclin G1 silencing, increases sensitivity to doxorubicin challenge. (aacrjournals.org)
  • In patients resected for HCC, lower miR-122 levels were associated with a shorter TTR, whereas higher cyclin G1 expression was related to a lower survival, suggesting that miR-122 might represent an effective molecular target for HCC. (aacrjournals.org)
  • Although PTEN signaling directly regulates p27 KIP1 levels in some settings, in endometrial carcinoma cells, PTEN expression indirectly regulated p27 KIP1 activity by modulating levels of cyclin D3. (aacrjournals.org)
  • In agreement with earlier studies cyclin D1 and E expression varied over an approximately tenfold range among the 18 cell lines studied. (garvan.org.au)
  • We have also seen an increase in the expression of proliferating cell nuclear antigen and G1/S cyclins during tumor development. (begellhouse.com)
  • Expression of cyclin D1 and cyclin D3 was deregulated in RA-resistant HBE cells , directly implicating these species in RA response . (dartmouth.edu)
  • Repression of each D-type cyclin expression independently suppressed HBE growth . (dartmouth.edu)
  • A synthetic triterponoid , 2-cyano-3,12-dioxooleana-1,9-then-28-oic acid (CDDO) , was found to repress effectively exogenous cyclin E expression . (dartmouth.edu)
  • The gain of cytosolic DEDD enhances cyclin D1 expression by interacting with heat shock 71 kDa protein 8 (HSC70). (nature.com)
  • The expression of cyclin G1 was silenced by transfection of cyclin G1-siRNA into HepG2 cells, and the expression of cyclin G1 mRNA and protein was measured by qRT-PCR and western blot analysis. (researchsquare.com)
  • The expression of cyclin G1 mRNA and protein in HepG2-cyclin G1-siRNA cells is significantly decreased compared with that in HepG2 cells. (researchsquare.com)
  • Silencing the expression of cyclin G1 inhibits the proliferation of HCC cells and enhances the radiosensitivity of HepG2 cells in vitro and in vivo. (researchsquare.com)
  • HepG2-cyclin G1-siRNA significantly decreases Bcl-2 expression and increases Bax expression in cells. (researchsquare.com)
  • Knockdown of cyclin G1 expression significantly decreased Bcl-2 expression, and increased Bax expression and caspase-3 activity in HCC cells. (allenpress.com)
  • Expression of human cyclins through the cell cycle . (wikipedia.org)
  • [5] ) The oscillations of the cyclins, namely fluctuations in cyclin gene expression and destruction by the ubiquitin mediated proteasome pathway, induce oscillations in Cdk activity to drive the cell cycle. (wikipedia.org)
  • The gene expression pattern of A2V6 mRNA in human tissues was noticeably different from that of wild-type cyclin A2 A2WT mRNA. (duhnnae.com)
  • Effects of adenoviruses expressing cdk-6, cdk-4, and cyclin D 1 on proliferation in human β-cells were studied in both invitro and in vivo models. (diabetesjournals.org)
  • Furthermore, we show that cdk-6 and a d -cyclin partner can be used to markedly accelerate replication in human β-cells in vitro. (diabetesjournals.org)
  • 1X10^6 MCF-7 cells were stained with 0.2ug Cyclin E antibody (11554-1-AP, red) and control antibody (blue). (cosmobio.co.jp)
  • WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells. (cosmobio.co.jp)
  • Low-molecular-weight cyclin E (LMW-E) in breast cancer cells induces genomic instability and resistance to inhibition by p21, p27 , and fulvestrant therapy. (aacrjournals.org)
  • Treatment of the cells with roscovitine overcomes the LMW cyclin E-mediated letrozole resistance. (aacrjournals.org)
  • Yeast cells become committed to the mitotic cell cycle at a stage during G1 called Start. (ox.ac.uk)
  • The aim of this study was to investigate the role of cyclin G1 in the radiotherapy of HCC cells. (researchsquare.com)
  • Cyclins can be divided into four classes based on their behavior in the cell cycle of vertebrate somatic cells and yeast cells: G1 cyclins, G1/S cyclins, S cyclins, and M cyclins. (wikipedia.org)
  • Depletion of cyclin G1 by interference RNA revealed that cyclin G1 regulated transcription of cyclin B1 in a p53-independent manner, and confirmed that the increased mitotic cells and cell death by cyclin G1 were dependent upon cyclin B1. (elsevier.com)
  • Cyclin E2 mRNA levels in human cells oscillate throughout the cell cycle and peak at the G1/S boundary, in parallel with the cyclin E mRNA. (elsevier.com)
  • The protein encoded by this gene is a member of the cyclin family and contains the cyclin box. (lsbio.com)
  • Cyclin G1 was a novel member of the cyclin family, and it is abnormally expressed in HCC. (researchsquare.com)
  • Genetic evidence suggested that the inhibition of mitotic cyclin-dependent kinase activities was dependent on and possibly mediated through the CDC6 gene product. (mysciencework.com)
  • Enhanced radiosensitivity by cyclin G1 was correlated with increased cyclin B1, CDC2/cyclin B1 complex, and MPM2. (elsevier.com)
  • Thus, regulated mitotic proteolysis of Clb3 is specifically required to make passage of Start in the succeeding cell cycle "memoryless"-dependent on conditions within that cycle, and independent of events such as B-type cyclin accumulation that occurred in the preceding cycle. (genetics.org)
  • It is thought that Start is triggered by the accumulation of G1 cyclins that bind to the CDC28 kinase and activate it. (ox.ac.uk)
  • So what determines the accumulation of G1 cyclins? (ox.ac.uk)
  • Contrary to full-length cyclin A2, which regulates cell proliferation, the A2V6 splice variant might play a role in regulating nondividing cell states such as terminal differentiation or senescence. (duhnnae.com)
  • The cyclin subunit imparts substrate specificity to the complex. (rcsb.org)
  • The catalytic subunit for cyclin A and B is Cdc2 p34 kinase. (acris-antibodies.com)
  • These data suggest that the role of cyclin CycMs3 differs from that of CycMs1 and CycMs2. (plantcell.org)
  • The role of cyclin E in lung carcinogenesis was explored in vitro and in vivo . (dartmouth.edu)
  • Human G1/S-specific cyclin-D1 has a predicted length of 295 residues and MW of 34 kDa. (biosensis.com)
  • Previous work in many organisms has indicated that B-type cyclin-dependent inhibition of mitotic exit imposes a requirement for mitotic destruction of B-type cyclins. (genetics.org)
  • Their activation at START depends primarily on the cyclin/cyclin-dependent kinase (CDK) complex Cln3-Cdc28. (biomedcentral.com)
  • Upon prolonged exposer to THTMP, all glioma cell lines undergo p53 and cyclin-dependent kinase mediated cell death with the IC50 concentration of 26.5 and 75.4 μM in LN229 and Snb19, respectively. (tut.fi)
  • G1 cyclins do not behave like the other cyclins, in that the concentrations increase gradually (with no oscillation), throughout the cell cycle based on cell growth and the external growth-regulatory signals. (wikipedia.org)
  • Abundant data in mice have demonstrated that the "G1/S checkpoint," or the "G1/S pathway" (supplemental Fig. 1, available in an online appendix at http://diabetes.diabetesjournals.org/cgi/content/full/db08-0631/DC1 ), in the cell cycle is critical in regulating rodent β-cell replication and physiological function (rev. in 17 - 26 ). (diabetesjournals.org)
  • The amount of cyclin E protein in the cell is controlled by ubiquitin-mediated proteolysis (see Woo, 2003).This pathway has not yet been annotated in Reactome. (reactome.org)
  • Future studies will further understand the signaling pathway involved in RA regulation of G1 cyclins . (dartmouth.edu)
  • M cyclin concentrations rise as the cell begins to enter mitosis and the concentrations peak at metaphase. (wikipedia.org)
  • The destruction of M cyclins during metaphase and anaphase, after the Spindle Assembly Checkpoint is satisfied, causes the exit of mitosis and cytokinesis. (wikipedia.org)
  • Cyclin E2-associated kinase activity rises in late G1 and peaks very close to cyclin E activity. (elsevier.com)
  • Our findings reveal a novel role for a eukaryotic G(1) cyclin in cytoplasmic organelle biogenesis and segregation. (mysciencework.com)
  • The central region of the predicted amino acid sequence of the cycMs3 gene is most similar to the cyclin box of yeast B-type and mammalian A- and B-type cyclins. (plantcell.org)
  • Mammalian G1 cyclins. (springer.com)
  • Kaposi sarcoma herpesvirus ( KSHV ) encodes a D-type cyclin (ORF72) that binds CDK6 and is likely to contribute to KSHV-related cancers [9] . (wikipedia.org)
  • Whi3 is an RNA-binding protein associated with the endoplasmic reticulum (ER) that binds the CLN3 mRNA and plays a key role in the efficient retention of cyclin Cln3 at the ER. (udl.cat)
  • Glycogen synthesis kinase 3 (GSK3) is critical for RA regulation of cyclin D1 but not cyclin D3 . (dartmouth.edu)
  • Control of cell cycle transcription during G1 and S phases. (springer.com)
  • Mutations in RAD27 define a potential link between G1 cyclins and DNA replication. (asm.org)
  • Previous genetic analysis has revealed that two B-type cyclins, Clb5 and Clb6, have a positive role in DNA replication. (mysciencework.com)
  • S cyclins bind to Cdk and the complex directly induces DNA replication. (wikipedia.org)
  • A protein that appears to hold the cell in G1 until any DNA damage is repaired, preventing replication of damaged DNA. (studystack.com)
  • Levels of cyclin E fall, whilst the levels of cyclin B begins to rise. (studystack.com)
  • Human islets transduced with cdk-6 and cyclin D 1 were transplanted into diabetic NOD-SCID mice and markedly outperformed native human islets in vivo, maintaining glucose control for the entire 6 weeks of the study. (diabetesjournals.org)
  • KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Cyclin G1. (antikoerper-online.de)
  • Immunoblots for the G1/S control molecules in the human islet. (diabetesjournals.org)
  • Miscellaneous other G1/S molecules (note that HDM2 is the human homolog of murine MDM2, the ubiquitin ligase for p53). (diabetesjournals.org)
  • cdk-4, cdk-6, and cyclin D 1 were overexpressed in human islets by their cognate virus but not in human islets transduced with Ad.lacZ or in normal human islets. (diabetesjournals.org)
  • Examples of isolated human islets stained for insulin (green) and BrdU (red) 3 days after transduction with adenoviruses encoding cdk-6 plus cyclin D 1 . (diabetesjournals.org)
  • CONCLUSIONS The human G1/S proteome is described for the first time. (diabetesjournals.org)
  • We therefore sought to catalog in the human islet the molecules that control the G1/S checkpoint of the cell cycle. (diabetesjournals.org)
  • Synthetic peptide from human Cyclin G. (lsbio.com)
  • Recombinant human Cyclin E (CCNE1) protein fragment (apprx. (enquirebio.com)
  • We report here the cloning and characterization of human and mouse cyclin E2, which define a new subfamily within the vertebrate E-type cyclins, while all previously identified family-members belong to the cyclin E1 subfamily. (elsevier.com)
  • The alternative splicing of human cyclin A2 has not yet been studied. (duhnnae.com)
  • Sequence-specific primers were designed to amplify various exon-intron regions of cyclin A2 mRNA in cell lines and human tissues. (duhnnae.com)
  • This study has revealed that some highly differentiated human tissues express an intron-retaining cyclin A2 mRNA that induced a G1-S block in vitro. (duhnnae.com)

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