Cyclin g. Medical search

A cyclin G subtype that is constitutively expressed throughout the cell cycle. Cyclin G1 is considered a major transcriptional target of TUMOR SUPPRESSOR PROTEIN P53 and is highly induced in response to DNA damage.

A cyclin subtype that is found associated with CYCLIN-DEPENDENT KINASE 5; cyclin G associated kinase, and PROTEIN PHOSPHATASE 2.

Protein encoded by the bcl-1 gene which plays a critical role in regulating the cell cycle. Overexpression of cyclin D1 is the result of bcl-1 rearrangement, a t(11;14) translocation, and is implicated in various neoplasms.

A cyclin subtype that has specificity for CDC2 PROTEIN KINASE and CYCLIN-DEPENDENT KINASE 2. It plays a role in progression of the CELL CYCLE through G1/S and G2/M phase transitions.

A 50-kDa protein that complexes with CYCLIN-DEPENDENT KINASE 2 in the late G1 phase of the cell cycle.

A cyclin subtype that is transported into the CELL NUCLEUS at the end of the G2 PHASE. It stimulates the G2/M phase transition by activating CDC2 PROTEIN KINASE.

A cyclin B subtype that colocalizes with MICROTUBULES during INTERPHASE and is transported into the CELL NUCLEUS at the end of the G2 PHASE.

A cyclin D subtype which is regulated by GATA4 TRANSCRIPTION FACTOR. Experiments using KNOCKOUT MICE suggest a role for cyclin D2 in granulosa cell proliferation and gonadal development.

A broadly expressed type D cyclin. Experiments using KNOCKOUT MICE suggest a role for cyclin D3 in LYMPHOCYTE development.

A cyclin A subtype primarily found in male GERM CELLS. It may play a role in the passage of SPERMATOCYTES into meiosis I.

A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. They generally function as ENZYME ACTIVATORS that drive the CELL CYCLE through transitions between phases. A subset of cyclins may also function as transcriptional regulators.

A widely-expressed cyclin A subtype that functions during the G1/S and G2/M transitions of the CELL CYCLE.

A cyclin subtype that is specific for CYCLIN-DEPENDENT KINASE 4 and CYCLIN-DEPENDENT KINASE 6. Unlike most cyclins, cyclin D expression is not cyclical, but rather it is expressed in response to proliferative signals. Cyclin D may therefore play a role in cellular responses to mitogenic signals.

An unusual cyclin subtype that is found highly expressed in terminally differentiated cells. Unlike conventional cyclins increased expression of cyclin G2 is believed to cause a withdrawal of cells from the CELL CYCLE.

A cyclin subtype that binds to the CYCLIN-DEPENDENT KINASE 3 and CYCLIN-DEPENDENT KINASE 8. Cyclin C plays a dual role as a transcriptional regulator and a G1 phase CELL CYCLE regulator.

The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.

Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events.

A cyclin B subtype that colocalizes with GOLGI APPARATUS during INTERPHASE and is transported into the CELL NUCLEUS at the end of the G2 PHASE.

A key regulator of CELL CYCLE progression. It partners with CYCLIN E to regulate entry into S PHASE and also interacts with CYCLIN A to phosphorylate RETINOBLASTOMA PROTEIN. Its activity is inhibited by CYCLIN-DEPENDENT KINASE INHIBITOR P27 and CYCLIN-DEPENDENT KINASE INHIBITOR P21.

A cyclin subtype that is found associated with CYCLIN-DEPENDENT KINASE 9. Unlike traditional cyclins, which regulate the CELL CYCLE, type T cyclins appear to regulate transcription and are components of positive transcriptional elongation factor B.

A cyclin subtype that is found as a component of a heterotrimeric complex containing cyclin-dependent kinase 7 and CDK-activating kinase assembly factor. The complex plays a role in cellular proliferation by phosphorylating several CYCLIN DEPENDENT KINASES at specific regulatory threonine sites.

The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors.

Cyclin-dependent kinase 4 is a key regulator of G1 PHASE of the CELL CYCLE. It partners with CYCLIN D to phosphorylate RETINOBLASTOMA PROTEIN. CDK4 activity is inhibited by CYCLIN-DEPENDENT KINASE INHIBITOR P16.

A family of cell cycle-dependent kinases that are related in structure to CDC28 PROTEIN KINASE; S CEREVISIAE; and the CDC2 PROTEIN KINASE found in mammalian species.

Phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the CELL CYCLE. It is the catalytic subunit of the MATURATION-PROMOTING FACTOR and complexes with both CYCLIN A and CYCLIN B in mammalian cells. The maximal activity of cyclin-dependent kinase 1 is achieved when it is fully dephosphorylated.

Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.

Product of the retinoblastoma tumor suppressor gene. It is a nuclear phosphoprotein hypothesized to normally act as an inhibitor of cell proliferation. Rb protein is absent in retinoblastoma cell lines. It also has been shown to form complexes with the adenovirus E1A protein, the SV40 T antigen, and the human papilloma virus E7 protein.

A phosphoprotein phosphatase subtype that is comprised of a catalytic subunit and two different regulatory subunits. At least two genes encode isoforms of the protein phosphatase catalytic subunit, while several isoforms of regulatory subunits exist due to the presence of multiple genes and the alternative splicing of their mRNAs. Protein phosphatase 2 acts on a broad variety of cellular proteins and may play a role as a regulator of intracellular signaling processes.

Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.

The period of the CELL CYCLE following DNA synthesis (S PHASE) and preceding M PHASE (cell division phase). The CHROMOSOMES are tetraploid in this point.

Proteins that control the CELL DIVISION CYCLE. This family of proteins includes a wide variety of classes, including CYCLIN-DEPENDENT KINASES, mitogen-activated kinases, CYCLINS, and PHOSPHOPROTEIN PHOSPHATASES as well as their putative substrates such as chromatin-associated proteins, CYTOSKELETAL PROTEINS, and TRANSCRIPTION FACTORS.

A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.

A cyclin-dependent kinase inhibitor that coordinates the activation of CYCLIN and CYCLIN-DEPENDENT KINASES during the CELL CYCLE. It interacts with active CYCLIN D complexed to CYCLIN-DEPENDENT KINASE 4 in proliferating cells, while in arrested cells it binds and inhibits CYCLIN E complexed to CYCLIN-DEPENDENT KINASE 2.

A cyclin subtype that is found abundantly in post-mitotic tissues. In contrast to the classical cyclins, its level does not fluctuate during the cell cycle.

A cyclin-dependent kinase inhibitor that mediates TUMOR SUPPRESSOR PROTEIN P53-dependent CELL CYCLE arrest. p21 interacts with a range of CYCLIN-DEPENDENT KINASES and associates with PROLIFERATING CELL NUCLEAR ANTIGEN and CASPASE 3.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

A cell line derived from cultured tumor cells.

The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.

A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)

Proteins coded by oncogenes. They include proteins resulting from the fusion of an oncogene and another gene (ONCOGENE PROTEINS, FUSION).

The B-cell leukemia/lymphoma-1 genes, associated with various neoplasms when overexpressed. Overexpression results from the t(11;14) translocation, which is characteristic of mantle zone-derived B-cell lymphomas. The human c-bcl-1 gene is located at 11q13 on the long arm of chromosome 11.

A subunit of the interleukin-12 receptor. It plays a role in receptor signaling by associating with JANUS KINASE 2.

An E3 UBIQUITIN LIGASE that interacts with and inhibits TUMOR SUPPRESSOR PROTEIN P53. Its ability to ubiquitinate p53 is regulated by TUMOR SUPPRESSOR PROTEIN P14ARF.

All of the processes involved in increasing CELL NUMBER including CELL DIVISION.

Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.

Cyclin-dependent kinase 6 associates with CYCLIN D and phosphorylates RETINOBLASTOMA PROTEIN during G1 PHASE of the CELL CYCLE. It helps regulate the transition to S PHASE and its kinase activity is inhibited by CYCLIN-DEPENDENT KINASE INHIBITOR P18.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

An adenocarcinoma containing finger-like processes of vascular connective tissue covered by neoplastic epithelium, projecting into cysts or the cavity of glands or follicles. It occurs most frequently in the ovary and thyroid gland. (Stedman, 25th ed)

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Established cell cultures that have the potential to propagate indefinitely.

A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.

A family of structurally-related proteins that were originally identified by their ability to complex with cyclin proteins (CYCLINS). They share a common domain that binds specifically to F-BOX MOTIFS. They take part in SKP CULLIN F-BOX PROTEIN LIGASES, where they can bind to a variety of F-BOX PROTEINS.

Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.

Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.

Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)

Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.

A structurally-diverse family of intracellular-signaling adaptor proteins that selectively tether specific protein kinase A subtypes to distinct subcellular sites. They play a role in focusing the PROTEIN KINASE A activity toward relevant substrates. Over fifty members of this family exist, most of which bind specifically to regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASE TYPE II such as CAMP PROTEIN KINASE RIIALPHA or CAMP PROTEIN KINASE RIIBETA.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.

Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.

Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.

Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.

Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.

A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.

Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.

Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.

Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).

The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.

Control of cell cycle progression by c-Jun is p53 dependent. (1/114)

The c-jun proto-oncogene encodes a component of the mitogen-inducible immediate-early transcription factor AP-1 and has been implicated as a positive regulator of cell proliferation and G1-to-S-phase progression. Here we report that fibroblasts derived from c-jun-/- mouse fetuses exhibit a severe proliferation defect and undergo a prolonged crisis before spontaneous immortalization. The cyclin D1- and cyclin E-dependent kinases (CDKs) and transcription factor E2F are poorly activated, resulting in inefficient G1-to-S-phase progression. Furthermore, the absence of c-Jun results in elevated expression of the tumor suppressor gene p53 and its target gene, the CDK inhibitor p21, whereas overexpression of c-Jun represses p53 and p21 expression and accelerates cell proliferation. Surprisingly, protein stabilization, the common mechanism of p53 regulation, is not involved in up-regulation of p53 in c-jun-/- fibroblasts. Rather, c-Jun regulates transcription of p53 negatively by direct binding to a variant AP-1 site in the p53 promoter. Importantly, deletion of p53 abrogates all defects of cells lacking c-Jun in cell cycle progression, proliferation, immortalization, and activation of G1 CDKs and E2F. These results demonstrate that an essential, rate-limiting function of c-Jun in fibroblast proliferation is negative regulation of p53 expression, and establish a mechanistic link between c-Jun-dependent mitogenic signaling and cell-cycle regulation. (+info)

Altered regulation of cyclin G in human breast cancer and its specific localization at replication foci in response to DNA damage in p53+/+ cells. (2/114)

Cyclin G, a recent addition to the cyclin family, was initially identified in screens for new src kinase family members and soon thereafter by differential screening for transcriptional targets of the tumor suppressor gene, p53. We have identified cyclin G as being overexpressed in breast and prostate cancer cells using differential display polymerase chain reaction screening. We demonstrate here that cyclin G is overexpressed in human breast and prostate cancer cells and in cancer cells in situ from tumor specimens. Cyclin G expression was tightly regulated throughout the cell cycle in normal breast cells, peaking at the S and G2/M phases of the cell cycle with lower levels in G1. The cell cycle-dependent expression was absent in breast cancer cells. Following DNA damage in normal p53+/+ cells, cyclin G is triggered to cluster in discrete nuclear DNA replication foci that contain replication-associated proteins such as proliferating cell nuclear antigen (PCNA). While p53-/- cells displayed a faint cyclin G nuclear staining pattern, there was no increased expression and no change in distribution of the staining pattern after DNA damage. The specific subcellular localization of cyclin G at DNA replication foci provides an additional link between p53-mediated growth arrest and cell cycle regulation and suggests that cyclin G may act as an effector of p53-mediated events by functional association with replication foci protein(s). (+info)

A G1 cyclin is necessary for maintenance of filamentous growth in Candida albicans. (3/114)

Candida albicans undergoes a dramatic morphological transition in response to various growth conditions. This ability to switch from a yeast form to a hyphal form is required for its pathogenicity. The intractability of Candida to traditional genetic approaches has hampered the study of the molecular mechanism governing this developmental switch. Our approach is to use the more genetically tractable yeast Saccharomyces cerevisiae to yield clues about the molecular control of filamentation for further studies in Candida. G1 cyclins Cln1 and Cln2 have been implicated in the control of morphogenesis in S. cerevisiae. We show that C. albicans CLN1 (CaCLN1) has the same cell cycle-specific expression pattern as CLN1 and CLN2 of S. cerevisiae. To investigate whether G1 cyclins are similarly involved in the regulation of cell morphogenesis during the yeast-to-hypha transition of C. albicans, we mutated CaCLN1. Cacln1/Cacln1 cells were found to be slower than wild-type cells in cell cycle progression. The Cacln1/Cacln1 mutants were also defective in hyphal colony formation on several solid media. Furthermore, while mutant strains developed germ tubes under several hypha-inducing conditions, they were unable to maintain the hyphal growth mode in a synthetic hypha-inducing liquid medium and were deficient in the expression of hypha-specific genes in this medium. Our results suggest that CaCln1 may coordinately regulate hyphal development with signal transduction pathways in response to various environmental cues. (+info)

G1 checkpoint protein and p53 abnormalities occur in most invasive transitional cell carcinomas of the urinary bladder. (4/114)

The G1 cell cycle checkpoint regulates entry into S phase for normal cells. Components of the G1 checkpoint, including retinoblastoma (Rb) protein, cyclin D1 and p16INK4a, are commonly altered in human malignancies, abrogating cell cycle control. Using immunohistochemistry, we examined 79 invasive transitional cell carcinomas of the urinary bladder treated by cystectomy, for loss of Rb or p16INK4a protein and for cyclin D1 overexpression. As p53 is also involved in cell cycle control, its expression was studied as well. Rb protein loss occurred in 23/79 cases (29%); it was inversely correlated with loss of p16INK4a, which occurred in 15/79 cases (19%). One biphenotypic case, with Rb+p16- and Rb-p16+ areas, was identified as well. Cyclin D1 was overexpressed in 21/79 carcinomas (27%), all of which retained Rb protein. Fifty of 79 tumours (63%) showed aberrant accumulation of p53 protein; p53 staining did not correlate with Rb, p16INK4a, or cyclin D1 status. Overall, 70% of bladder carcinomas showed abnormalities in one or more of the intrinsic proteins of the G1 checkpoint (Rb, p16INK4a and cyclin D1). Only 15% of all bladder carcinomas (12/79) showed a normal phenotype for all four proteins. In a multivariate survival analysis, cyclin D1 overexpression was linked to less aggressive disease and relatively favourable outcome. In our series, Rb, p16INK4a and p53 status did not reach statistical significance as prognostic factors. In conclusion, G1 restriction point defects can be identified in the majority of bladder carcinomas. Our findings support the hypothesis that cyclin D1 and p16INK4a can cooperate to dysregulate the cell cycle, but that loss of Rb protein abolishes the G1 checkpoint completely, removing any selective advantage for cells that alter additional cell cycle proteins. (+info)

Recovery of the yeast cell cycle from heat shock-induced G(1) arrest involves a positive regulation of G(1) cyclin expression by the S phase cyclin Clb5. (5/114)

In the yeast Saccharomyces cerevisiae, heat shock stress induces a variety of cellular responses including a transient cell cycle arrest before G(1)/S transition. Previous studies have suggested that this G(1) delay is probably attributable to a reduced level of the G(1) cyclin gene (CLN1 and CLN2) transcripts. Here we report our finding that the G(1) cyclin Cln3 and the S cyclin Clb5 are the key factors required for recovery from heat shock-induced G(1) arrest. Heat shock treatment of G(1) cells lacking either CLN3 or CLB5/CLB6 functions leads to prolonged cell cycle arrest before the initiation of DNA synthesis, concomitant with a severe deficiency in bud formation. The inability of the clb5 clb6 mutant to resume normal budding after heat shock treatment is unanticipated, since the S phase cyclins are generally thought to be required mainly for initiation of DNA synthesis and have no significant roles in bud formation in the presence of functional G(1) cyclins. Further studies reveal that the accumulation of G(1) cyclin transcripts is markedly delayed in the clb5 clb6 mutant following heat shock treatment, indicating that the CLN gene expression may require Clb5/Clb6 to attain a threshold level for driving the cell cycle through G(1)/S transition. Consistent with this assumption, overproduction of Clb5 greatly enhances the transcription of at least two G(1) cyclin genes (CLN1 and CLN2) in heat-shocked G(1) cells. These results suggest that Clb5 may positively regulate the expression of G(1) cyclins during cellular recovery from heat shock-induced G(1) arrest. Additional evidence is presented to support a role for Clb5 in maintaining the synchrony between budding and DNA synthesis during normal cell division as well. (+info)

Ectopic expression of Cdc25A accelerates the G(1)/S transition and leads to premature activation of cyclin E- and cyclin A-dependent kinases. (6/114)

Human Cdc25 phosphatases play important roles in cell cycle regulation by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases. Three human Cdc25 isoforms, A, B, and C, have been discovered. Cdc25B and Cdc25C play crucial roles at the G(2)/M transition. In the present study, we have investigated the function of human Cdc25A phosphatase. Cell lines that express human Cdc25A in an inducible manner have been generated. Ectopic expression of Cdc25A accelerates the G(1)/S-phase transition, indicating that Cdc25A controls an event(s) that is rate limiting for entry into S phase. Furthermore, we carried out a detailed analysis of the expression and activation of human Cdc25A. Activation of endogenous Cdc25A occurs during late G(1) phase and increases in S and G(2) phases. We further demonstrate that Cdc25A is activated at the same time as cyclin E- and cyclin A-dependent kinases. In vitro, Cdc25A dephosphorylates and activates the cyclin-Cdk complexes that are active during G(1). Overexpression of Cdc25A in the inducible system, however, leads to a premature activation of both cyclin E-Cdk2 and cyclin A-Cdk2 complexes, while no effect of cyclin D-dependent kinases is observed. Furthermore, Cdc25A overexpression induces a tyrosine dephosphorylation of Cdk2. These results suggest that Cdc25A is an important regulator of the G(1)/S-phase transition and that cyclin E- and cyclin A-dependent kinases act as direct targets. (+info)

A role of cyclin G in the process of apoptosis. (7/114)

Cyclin G was previously identified as a target gene of the p53 tumor suppresser protein, and levels of cyclin G are increased after induction of p53 by DNA damage. However, the function of cyclin G has not been established. To determine the effect of increased expression of cyclin G, retroviruses encoding cyclin G were constructed and used to infect three different murine cell lines. Cyclin G protein levels induced by the retroviruses were within the range seen after DNA damage induction of p53. In each case we observed that such over-expression of cyclin G augments the apoptotic process. TNF-alpha induction of apoptosis is increased by expression of cyclin G in NIH3T3 fibroblasts which express p53, as well as in 10.1 fibroblasts which contain no p53 allele. Additionally, we observed that while cyclin G expression is markedly reduced upon aggregate formation in embryonic carcinoma P19 cells, retrovirus-mediated over-expression of cyclin G enhances apoptotic cell death in aggregated P19 cells, and increases the extent of apoptosis caused by retinoic acid or serum starvation of these cells. These data demonstrate that cyclin G plays a facilitating role in modulating apoptosis induced by different stimuli. Moreover, we have discovered that cyclin G expression is rapidly induced in P19 cells after exposure to Bone Morphogenic Protein-4 (BMP-4), suggesting that cyclin G may mediate apoptotic signals generated by BMP-4. (+info)

Interferon-alpha inhibits proliferation in human T lymphocytes by abrogation of interleukin 2-induced changes in cell cycle-regulatory proteins. (8/114)

IFN-alpha exerts prominent regulatory functions on the immune system. One such effect is the inhibition of proliferation of in vitro stimulated T lymphocytes. The exact physiological function of this activity is not known, but it has been implicated in the antiviral effects of IFN, its antitumor action in T-cell malignancies, and the regulation of the in vivo T-cell response. Here, we have investigated the mechanism underlying the IFN-alpha-mediated growth inhibition of normal human PHA- and IL-2-stimulated T lymphocytes by an analysis of how IFN-alpha treatment influences known molecular events that normally accompany the transition from quiescence to proliferation in these cells. IFN-alpha treatment was found to profoundly block S-phase entry of stimulated T lymphocytes. This correlated with a strong inhibition of IL-2-induced changes in G1-regulatory proteins, including the prevented up-regulation of G1 cyclins and cyclin-dependent kinases as well as an abrogation of mitogen-induced reduction of p27Kip1 levels. This latter effect was due to a maintained stability of the p27Kip1 protein in the IFN-alpha-treated cells. In line with these findings, phosphorylation of the pocket proteins was abrogated in IFN-alpha-treated cells. Furthermore, our data indicate that IFN-alpha has selective effects on the pathways that emerge from the IL-2 receptor because IFN-alpha treatment does not block IL-2-induced up-regulation of c-myc or Cdc25A. (+info)

... D / CDK4, Cyclin D / CDK6, and Cyclin E / CDK2 - regulates transition from G1 to S phase. G2/M cyclins - essential for ... The rise in presence of G1/S cyclins is paralleled by a rise in S cyclins. G1 cyclins do not behave like the other cyclins, in ... G1 cyclins, G1/S cyclins, S cyclins, and M cyclins. This division is useful when talking about most cell cycles, but it is not ... Note that the cyclins are now classified according to their conserved cyclin box structure, and not all these cyclins alter in ...

https://en.wikipedia.org/wiki/Cyclin

Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which ... Cyclin-T2 is a protein that in humans is encoded by the CCNT2 gene. The protein encoded by this gene belongs to the highly ... This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb ... "Entrez Gene: CCNT2 cyclin T2". Simone C, Bagella L, Bellan C, Giordano A (Jun 2002). "Physical interaction between pRb and cdk9 ...

https://en.wikipedia.org/wiki/Cyclin_T2

Cyclin-O is a protein that in humans is encoded by the CCNO gene. Cyclin O has been shown to interact with RPA2 and PCNA. ... "Entrez Gene: CCNO cyclin O". Otterlei M, Warbrick E, Nagelhus TA, Haug T, Slupphaug G, Akbari M, Aas PA, Steinsbekk K, Bakke O ... Hirst R, Gosden R, Miller D (June 2006). "The cyclin-like uracil DNA glycosylase (UDG) of murine oocytes and its relationship ... Muller SJ, Caradonna S (January 1993). "Cell cycle regulation of a human cyclin-like gene encoding uracil-DNA glycosylase". The ...

https://en.wikipedia.org/wiki/Cyclin_O

Cyclins function as activating subunits of enzymatic complex together with cyclin-dependent kinases (CDKs). Different cyclins ... Cyclin-A1 interacts with: CDC20, Cyclin-dependent kinase 2, E2F1, GNB2L1, GPS2, MYBL2, and Retinoblastoma protein. GRCh38: ... "Cyclin A1 directly interacts with B-myb and cyclin A1/cdk2 phosphorylate B-myb at functionally important serine and threonine ... "Cyclin A1 directly interacts with B-myb and cyclin A1/cdk2 phosphorylate B-myb at functionally important serine and threonine ...

https://en.wikipedia.org/wiki/Cyclin_A1

... is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with ... Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds ... Cyclin B2 has been shown to interact with TGF beta receptor 2. Cyclin B GRCh38: Ensembl release 89: ENSG00000157456 - Ensembl, ... "Cyclin B2-null mice develop normally and are fertile whereas cyclin B1-null mice die in utero". Proc. Natl. Acad. Sci. U.S.A. ...

https://en.wikipedia.org/wiki/Cyclin_B2

... is a member of the cyclin family. Cyclin B is a mitotic cyclin. The amount of cyclin B (which binds to Cdk1) and the ... Because cyclin B is necessary for cells to enter mitosis and therefore necessary for cell division, cyclin B levels are often ... The fact that cyclin B is often disregulated in cancer cells makes cyclin B an attractive biomarker. Many studies have been ... Cyclin+B at the US National Library of Medicine Medical Subject Headings (MeSH) Drosophila Cyclin B - The Interactive Fly (All ...

https://en.wikipedia.org/wiki/Cyclin_B

"Entrez Gene: CCNE1 cyclin E1". Shanahan F, Seghezzi W, Parry D, Mahony D, Lees E (February 1999). "Cyclin E associates with ... Mumberg D, Wick M, Bürger C, Haas K, Funk M, Müller R (1997). "Cyclin ET, a new splice variant of human cyclin E with a unique ... Cyclin E1 has been shown to interact with: CDC25A, CDKN1B, CUL3 Cdk1, Cyclin-dependent kinase 2, HERC5, P21, Retinoblastoma- ... Lew DJ, Dulić V, Reed SI (October 1991). "Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast ...

https://en.wikipedia.org/wiki/Cyclin_E1

Other than Rb, viral cyclin D-Cdk6 complex also targets p27Kip, a Cdk inhibitor of cyclin E and A. In addition, viral cyclin D- ... The phosphorylation of Rb by cyclin A-Cdk2, cyclin B-Cdk1, and cyclin E-Cdk2 are unaffected. The C terminus has a stretch of 21 ... In mice and humans, two more cyclin D proteins have been identified. The three homologues, called cyclin D1, cyclin D2, and ... among which is cyclin D. In this way, cyclin D is synthesized as long as the growth factor is present. Cyclin D levels in ...

https://en.wikipedia.org/wiki/Cyclin_D

Cyclins function as regulators of cyclin-dependent kinases. Different cyclins exhibit distinct expression and degradation ... Brooks AR, Shiffman D, Chan CS, Brooks EE, Milner PG (Apr 1996). "Functional analysis of the human cyclin D2 and cyclin D3 ... "The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2". The ... G1/S-specific cyclin-D2 is a protein that in humans is encoded by the CCND2 gene. The protein encoded by this gene belongs to ...

https://en.wikipedia.org/wiki/Cyclin_D2

Cyclins function as regulators of CDKs (Cyclin-dependent kinase). Different cyclins exhibit distinct expression and degradation ... cyclin D1 is translocated to the IgH promoter leading to cyclin D1 overexpression. Chromosomal translocation of the cyclin D1 ... Cyclin D1 is a protein that in humans is encoded by the CCND1 gene. The CCND1 gene encodes the cyclin D1 protein. The human ... Cyclin D1 and the mechanisms it regulates have the potential to be a therapeutic target for cancer drugs: Cyclin D1 has been ...

https://en.wikipedia.org/wiki/Cyclin_D1

"Cyclin K inhibits HIV-1 gene expression and replication by interfering with cyclin-dependent kinase 9 (CDK9)-cyclin T1 ... "Entrez Gene: CCNK cyclin K". Baek K, Brown RS, Birrane G, Ladias JA (February 2007). "Crystal structure of human cyclin K, a ... Cyclin K also interacts with HIV nef protein. In the presence of overexpressed Nef protein, Cyclin k and CDK9 binding is ... Cyclin K is indispensable for Leukemia growth. SETD1A, is also known to bind Cyclin K through its FLOS domain. The interaction ...

https://en.wikipedia.org/wiki/Cyclin_K

"Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction". EMBO J. 19 (6): 1378-88. doi: ... G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. Cyclin B1 is a regulatory protein ... Like all cyclins, levels of cyclin B1 oscillate over the course of the cell cycle. Just prior to mitosis, a large amount of ... Cyclin B1 can reside in the nucleus or the cytoplasm which can have an effect on the malignant potential of cyclin B1 when ...

https://en.wikipedia.org/wiki/Cyclin_B1

... is a protein that in humans is encoded by the CCNE2 gene. It is a G1 cyclin that binds Cdk2 and is inhibited by p27( ... Gudas JM, Payton M, Thukral S, Chen E, Bass M, Robinson MO, Coats S (January 1999). "Cyclin E2, a novel G1 cyclin that binds ... Zariwala, M.; Liu, J.; Xiong, Y. (1998-11-26). "Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is ...

https://en.wikipedia.org/wiki/Cyclin_E2

Like all cyclin family members, cyclin E forms a complex with cyclin-dependent kinase (CDK2). Cyclin E/CDK2 regulates multiple ... Cyclin E is a member of the cyclin family. Cyclin E binds to G1 phase Cdk2, which is required for the transition from G1 to S ... Cyclin E/CDK2 plays a critical role in the G1 phase and in the G1-S phase transition. Cyclin E/CDK2 phosphorylates ... Dysregulation of cyclin E occurs in 18-22% of the breast cancers. Cyclin E is a prognostic marker in breast cancer, its altered ...

https://en.wikipedia.org/wiki/Cyclin_E

Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns that ... Cyclin-T1 is a protein that in humans is encoded by the CCNT1 gene. The protein encoded by this gene belongs to the highly ... This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p- ... This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal ...

https://en.wikipedia.org/wiki/Cyclin_T1

Cyclin-A2 is a protein that in humans is encoded by the CCNA2 gene. It is one of the two types of cyclin A: cyclin A1 is ... Cyclin A2 belongs to the cyclin family, whose members regulate cell cycle progression by interacting with CDK kinases. Cyclin ... The cyclin A2-CDK2 complex eventually phosphorylates E2F, turning off cyclin A2 transcription. E2F promotes cyclin A2 ... Cyclin A2 is synthesized at the onset of S phase and localizes to the nucleus, where the cyclin A2-CDK2 complex is implicated ...

https://en.wikipedia.org/wiki/Cyclin_A2

... has been shown to interact with P53, Cyclin-dependent kinase 7 and MNAT1. GRCh38: Ensembl release 89: ENSG00000134480 ... Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which ... Cyclin-H is a protein that in humans is encoded by the CCNH gene. The protein encoded by this gene belongs to the highly ... This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and ...

https://en.wikipedia.org/wiki/Cyclin_H

... remains associated with CDK1 from late S into late G2 phase when it is replaced by cyclin B. Cyclin A/CDK1 is thought ... Cyclin A is the only cyclin that regulates multiple steps of the cell cycle. Cyclin A can regulate multiple cell cycle steps ... Cyclin A2 is expressed in dividing somatic cells. Cyclin A, along with the other members of the cyclin family, regulates cell ... P21 is a CDK inhibitor that binds to several cyclin/CDK complexes, including cyclin A-CDK2/1 and cyclin D/CDK4, and blocks the ...

https://en.wikipedia.org/wiki/Cyclin_A

Cyclin-dependent kinase 4, Cyclin-dependent kinase 6, EIF3K, and Retinoic acid receptor alpha. Cyclin Cyclin D GRCh38: Ensembl ... Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which ... Brooks AR, Shiffman D, Chan CS, Brooks EE, Milner PG (1996). "Functional analysis of the human cyclin D2 and cyclin D3 ... G1/S-specific cyclin-D3 is a protein that in humans is encoded by the CCND3 gene. The protein encoded by this gene belongs to ...

https://en.wikipedia.org/wiki/Cyclin_D3

CDK6; cyclin D1, cyclin D2, cyclin D3 CDK7; cyclin H CDK8; cyclin C CDK9; cyclin T1, cyclin T2a, cyclin T2b, cyclin K CDK10 ... cyclin A, cyclin B CDK2; cyclin A, cyclin E CDK3; cyclin C CDK4; cyclin D1, cyclin D2, cyclin D3 CDK5; CDK5R1, CDK5R2. See also ... Furthermore, cyclin binding determines the specificity of the cyclin-CDK complex for particular substrates. Cyclins can ... Viruses can encode proteins with sequence homology to cyclins. One much-studied example is K-cyclin (or v-cyclin) from Kaposi ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase

E2F.2FpRb complexes Hyperphosphorylation cdc25 Maturation promoting factor CDK cyclin A cyclin B cyclin D cyclin E Wee (cell ... "Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction". EMBO J. 19 (6): 1378-1388. doi: ... Cyclin binding alters access to the active site of Cdk1, allowing for Cdk1 activity; furthermore, cyclins impart specificity to ... Furthermore, cyclins can target Cdk1 to particular subcellular locations. In addition to regulation by cyclins, Cdk1 is ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_1

"Entrez Gene: CDK10 cyclin-dependent kinase (CDC2-like) 10". Kasten M, Giordano A (Apr 2001). "Cdk10, a Cdc2-related kinase, ... Cyclin-dependent kinase 10 has been shown to interact with ETS2. GRCh38: Ensembl release 89: ENSG00000185324 - Ensembl, May ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_10

A Cyclin-dependent kinase 6 interacts with: CDKN2C, Cyclin D1, Cyclin D3, P16, PPM1B, and PPP2CA. Cell cycle Cyclin-dependent ... It is regulated by cyclins, more specifically by Cyclin D proteins and Cyclin-dependent kinase inhibitor proteins. The protein ... 1996). "Inhibition of Cyclin D-CDK4/CDK6 Activity Is Associated with an E2F-Mediated Induction of Cyclin Kinase Inhibitor ... 2003). "Expression of Cyclin-Dependent Kinase 6, but Not Cyclin-Dependent Kinase 4, Alters Morphology of Cultured Mouse ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_6

... has been shown to interact with: BRCA1, CDK2AP1, CDKN1B CDKN3, CEBPA, Cyclin A1, Cyclin E1, Flap ... "Entrez Gene: CDK2 cyclin-dependent kinase 2". Echalier A, Endicott JA, Noble ME (March 2010). "Recent developments in cyclin- ... This protein associates with and is regulated by the regulatory subunits of the complex including cyclin E or A. Cyclin E binds ... Lacy S, Whyte P (May 1997). "Identification of a p130 domain mediating interactions with cyclin A/cdk 2 and cyclin E/cdk 2 ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_2

... has been shown to interact with: Androgen receptor, Cyclin H, GTF2H1, MNAT1, P53, SUPT5H, and XPB. ... Cyclin-dependent kinase 7, or cell division protein kinase 7, is an enzyme that in humans is encoded by the CDK7 gene. The ... The growth suppressor p53 has been shown to interact with cyclin H both in vitro and in vivo. Addition of wild type p53 was ... "Entrez Gene: CDK7 cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase)". Patel H, Abduljabbar R, Lai ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_7

"OMIM Entry - * 123831 - CYCLIN-DEPENDENT KINASE 5; CDK5". omim.org. Retrieved 2020-11-02. Tsai, Li-Huei. Cyclin Dependent ... Cyclin-dependent kinase 5 is a protein, and more specifically an enzyme, that is encoded by the Cdk5 gene. It was discovered 15 ... Cyclin Dependent Kinase 5. Springer. 19 August 2008. ISBN 978-0-387-78886-9. Patrick GN, Zukerberg L, Nikolic M, de la Monte S ... Cyclin-Dependent+Kinase+5 at the US National Library of Medicine Medical Subject Headings (MeSH) CDK5 human gene location in ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_5

During G2 phase, cyclin A is degraded, while cyclin B is synthesized and cyclin B-Cdk1 complexes form. Not only are cyclin B- ... cyclin-dependent kinase (CDK), with a regulatory subunit, cyclin. Once cyclin-dependent kinases bind to cyclin, the formed ... Cyclin Cyclin-dependent kinase Malumbres M, Barbacid M. Mammalian cyclin-dependent kinases. Trends Biochem. Sci. 2005 Nov;30(11 ... cyclin D1-Cdk4 and cyclin D1-Cdk6 phosphorylate pRB, followed by additional phosphorylation from the cyclin E-Cdk2 CDKC. Once ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_complex

"Entrez Gene: CDK4 cyclin-dependent kinase 4". "CDK4 - Cyclin-dependent kinase 4 - Homo sapiens (Human) - CDK4 gene & protein". ... Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 ... 1993). "Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent ... 1995). "Identification of human cyclin-dependent kinase 8, a putative protein kinase partner for cyclin C". Proc. Natl. Acad. ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_4

... or CDK9 is a cyclin-dependent kinase associated with P-TEFb. The protein encoded by this gene is a ... This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found ... Cyclin-Dependent+Kinase+9 at the US National Library of Medicine Medical Subject Headings (MeSH) Drosophila Cyclin dependent ... "Entrez Gene: CDK9 cyclin-dependent kinase 9 (CDC2-related kinase)". MacLachlan TK, Sang N, De Luca A, Puri PL, Levrero M, ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_9

"Entrez Gene: CDK3 cyclin-dependent kinase 3". Bullrich F, MacLachlan TK, Sang N, et al. (1995). "Chromosomal mapping of members ... 2002). "ik3-1/Cables is a substrate for cyclin-dependent kinase 3 (cdk 3)". Eur. J. Biochem. 268 (23): 6076-82. doi:10.1046/j. ... Ren S, Rollins BJ (2004). "Cyclin C/cdk3 promotes Rb-dependent G0 exit". Cell. 117 (2): 239-51. doi:10.1016/S0092-8674(04)00300 ... CDK3 can phosphorylate histone H1 and interacts with an unknown type of cyclin. GRCh38: Ensembl release 89: ENSG00000250506 - ...

https://en.wikipedia.org/wiki/Cyclin-dependent_kinase_3

Activation of cyclin D1 and D2 promoters by human T-cell leukemia virus type I tax protein is associated with IL-2-independent ... Our data suggest that induction of cyclins D1 and D2 by Tax is involved in IL-2-independent cell-cycle progression as well as ... A Tax mutant that did not activate NF-{kappa}B failed to activate cyclin D1 and D2 promoters. Inhibitors of NF-{kappa}B ( ... Our findings link HTLV-I infection to changes in cellular D-type cyclin gene expression, transformation of T cells and ...

https://www.mdc-berlin.de/de/research/publications/activation-cyclin-d1-and-d2-promoters-human-t-cell-leukemia-virus-type-i-tax

Rabbit polyclonal Cyclin A1 antibody. Validated in WB, ELISA, IHC, ICC/IF and tested in Human. Cited in 24 publication(s). ... I attach you a picture of wb in which the anti-cyclin A E23.1 ab38 is a good antibody to recognize the cyclin A2 on wb but the ... Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) *Research with confidence - consistent and reproducible results with ... and I have been to order the antibody anti-cyclin A1 ab53699 and anti-cyclin A E23.1 ab38 to abcam. I have a technical problem ...

https://www.abcam.com/cyclin-a1-antibody-ab53699.html

cyclin dependent kinase 5 regulatory subunit 2provided by HGNC. Primary source. HGNC:HGNC:1776 See related. Ensembl: ... enables cyclin-dependent protein serine/threonine kinase activator activity IBA Inferred from Biological aspect of Ancestor. ... CDK5R2 cyclin dependent kinase 5 regulatory subunit 2 [ Homo sapiens (human) ] Gene ID: 8941, updated on 22-Sep-2022 ... enables cyclin-dependent protein serine/threonine kinase activator activity ISS Inferred from Sequence or Structural Similarity ...

https://www.ncbi.nlm.nih.gov/gene/8941

J:306141 Franken GAC, et al., Cyclin M2 (CNNM2) knockout mice show mild hypomagnesaemia and developmental defects. Sci Rep. ...

http://www.informatics.jax.org/marker/MGI:2151060

Crystal structure of the Cyclin A-CDK2-ORC1 complex ... The complex of Cyclin A with cyclin-dependent kinase 2 (CDK2) ... The complex of Cyclin A with cyclin-dependent kinase 2 (CDK2) controls the DNA replication activity through phosphorylation of ... The structure revealed that the ORC1 peptide interacts with a hydrophobic groove, termed cyclin binding groove (CBG), of Cyclin ... Progression of cell cycle is regulated by sequential expression of cyclins, which associate with distinct cyclin kinases to ...

https://www.rcsb.org/structure/6P3W

Highly specific and rigorously validated in-house, Cyclin D2 (D52F9) Rabbit Monoclonal Antibody (CST #3741) is ready to ship. ... Monoclonal Antibody for studying Cyclin D2. Cited in 111 publications. Validated for Western Blotting, Immunoprecipitation. ... Cyclin D2 (D52F9) Rabbit mAb detects endogenous levels of total cyclin D2 protein. It does not cross-react with other family ... Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin ...

https://www.cellsignal.com/products/primary-antibodies/cyclin-d2-d52f9-rabbit-mab/3741

CDK4 functions as a cell-cycle initiator protein. This protein is coexpressed and copurified with CyclinD1.

https://www.thermofisher.com/order/catalog/product/PV4400

View Mouse Monoclonal anti-Cyclin D1 Antibody (SPM587) (NBP2-32840). Validated Applications: WB, Flow, ICC/IF, IHC, IHC-P. ... Cyclin D1 Antibody (SPM587) Summary. Immunogen. Human recombinant full length cyclin D1 protein (Uniprot: P24385) ... Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - analysis of Cyclin D1 in human breast cancer cell line MDA-MB-231 cell ... Diseases for Cyclin D1 Antibody (NBP2-32840). Discover more about diseases related to Cyclin D1 Antibody (NBP2-32840). ...

https://www.novusbio.com/products/cyclin-d1-antibody-spm587_nbp2-32840

... Published: 08th May 2020 ...

http://www.articlealley.com/roscovitine-a-particular-inhibitor-of-cyclin-dependent-kinases-and-cycloheximide-a-protein-synthesis-2444087.html

The cyclin-dependent kinase inhibitory domain of the yeast Sic1 protein is contained within the C-terminal 70 amino acids. ... BACKGROUND: The p13suc1 gene product is a member of the cks (cyclin-dependent protein kinase subunit) protein family and has ...

https://www.wikigenes.org/e/gene/e/2540239.html

Use of Cyclin-Dependent Kinase 4/6 Inhibitor with Hormonal Therapy in Metastatic Breast Cancer-Efficacy in Patient Subgroups * ... Use of Cyclin-Dependent Kinase 4/6 Inhibitor with Hormonal Therapy in Metastatic Breast Cancer-Efficacy in Patient Subgroups ... Cyclin-dependent kinases (CDKs) are enzymes that play an important role in cell division, making them attractive therapeutic ... Abbreviations: CDKI, cyclin-dependent kinase inhibitor; CI, confidence interval; MNE, median not estimable; NE, not estimable; ...

https://www.fda.gov/drugs/regulatory-science-research-and-education/use-cyclin-dependent-kinase-46-inhibitor-hormonal-therapy-metastatic-breast-cancer-efficacy-patient

... human and yeast cyclins, thus identifying p35 as a cyclin-like regulatory subunit. The greatest sequence similarity of human ... is itself a Cdc2-related cyclin-dependent protein kinase that associates with cyclin H. The present study utilized specific ... The activation of cyclin-dependent protein kinases (Cdks) is dependent upon site-specific phosphorylation and dephosphorylation ... cyclin H), and a 35-kDa protein that was further characterized herein. Microsequence analysis obtained after limited ...

https://www.semanticscholar.org/paper/Biochemical-Characterization-of-the-Human-Protein-Yee-Wu/38f78ad6da05dbe51ab7dc76766a699eee0c663c

IER3, BCL2A1 and cyclin D2 mRNA expression were detected using RT-qPCR, 48 h after transfection with miR-30a. As demonstrated ... Wang T, Li F and Tang S: MiR-30a upregulates BCL2A1, IER3 and cyclin D2 expression by targeting FOXL2. Oncol Lett 9: 967-971, ... miR-30a promotes BCL2A1, IER3 and cyclin D2 gene expression by suppressing FOXL2. The consequences of miR-30a knockdown and ... Wang, T., Li, F., Tang, S.MiR-30a upregulates BCL2A1, IER3 and cyclin D2 expression by targeting FOXL2. Oncology Letters 9.2 ...

https://www.spandidos-publications.com/10.3892/ol.2014.2723

... CCND2 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT ... Cyclins serve as regulators of CDK kinases. Various cyclins demonstrate distinct expression and degradation patterns which ... CCND2 is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein ...

https://www.neuromics.com/PR27281

CyclinA2-Cyclin-dependent Kinase Regulates SAMHD1 Protein Phosphohydrolase Domain.. Yan J, Hao C, DeLucia M, Swanson S, Florens ...

https://www.stowers.org/research/publications/libpb-774

Herein, we show that cyclin D1 enhanced H3K9 dimethylation though direct association with G9a. Endogenous cyclin D1 was ... The finding that cyclin D1 is required for recruitment of G9a to target genes in chromatin and for H3K9 dimethylation, ... The cyclin D1 gene product encodes the regulatory subunit of the holoenzyme that phosphorylates pRB and NRF1 thereby governing ... Herein, we show that cyclin D1 enhanced H3K9 dimethylation though direct association with G9a. Endogenous cyclin D1 was ...

https://jdc.jefferson.edu/cbfp/148/

3-[(6,7-Dimethoxy-4-quinazolinyl)amino]-phenol (Janex 3; WHI-P180) Cyclin-dependent Kinase (CDK) Inhibitor

https://otavachemicals.com/products/biochemicals/171-cyclin-dependentkinasecdkinhibitor

Suppression of cell transformation by the cyclin-dependent kinase inhibitor p57KIP2 requires binding to proliferating cell ...

https://www.addgene.org/browse/article/1921/

Proteintechs Mouse Monoclonal Cyclin B2 antibody is validated in IF and shows reactivity with Human, mouse samples. ... Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and ... Cyclin B2 serves a key role in progression of G2/M transition. Cyclin B2 has been found to be up-regulated in a variety of ... CoraLite®488-conjugated Cyclin B2 Monoclonal antibody. Cyclin B2 Monoclonal Antibody for IF. ...

https://www.ptglab.com/products/Cyclin-B2-Antibody-CL488-67726.htm

The level of cyclin-dependent kinase inhibitor p27 Kip1 after DNA damage is retained in chronic lymphocytic leukemia cells ...

https://www.muni.cz/uk/vyzkum/publikace/1399398

... cyclin D1 and c-myc, and the similar eye phenotypes of cyclin D1 and meis1 morphants, prompted us to ask whether cyclin D1 and ... meis1 morphants show a dramatic reduction in cyclin D1 and c-myc in the eye (red arrowheads). cyclin D1 and c-myc are still ... meis1 morphants show a dramatic reduction in cyclin D1 and c-myc in the eye (red arrowheads). cyclin D1 and c-myc are still ... Co-injection of meis1 mRNA rescues the loss of cyclin D1 expression in meis1-morphant embryos.cyclin D1 in situ hybridization ...

https://journals.biologists.com/dev/article/135/5/799/65019/meis1-regulates-cyclin-D1-and-c-mycexpression-and

... such as MYC and cyclin D1 (Shtutman et al., 1999; Tetsu and McCormick, 1999). The cyclin D1 protein is a major player in the ... 1999) Cyclins and cell cycle checkpoints Annual Review of Pharmacology and Toxicology 39:295-312. ... 2008) Transcriptional regulation of the cyclin D1 gene at a glance Journal of Cell Science 121:3853-3857. ... 2000) Beta-catenin, a novel prognostic marker for breast cancer: its roles in cyclin D1 expression and cancer progression PNAS ...

https://elifesciences.org/articles/16748

Kumagai, Akiko and Dunphy, William G. (1995) Control of the Cdc2/cyclin B complex in Xenopus egg extracts arrested at a G2/M ... Control of the Cdc2/cyclin B complex in Xenopus egg extracts arrested at a G2/M checkpoint with DNA synthesis inhibitors ... However, at threshold concentrations, a Cdc2/cyclin B complex containing a mutant Cdc2 subunit that cannot be phosphorylated on ... We provide evidence that the checkpoint-dependent suppression of the Cdc2/cyclin B complex involves a titratable inhibitor that ...

https://authors.library.caltech.edu/3895/

This was associated with a fall in cyclin D1 levels, a reduction in the half-life of cyclin D1 protein and a decline in cyclin ... and cyclin E-cyclin-dependent kinase (Cdk) 2 activity demonstrated that overexpression of cyclin D1 decreased sensitivity to ... and the maintenance of cyclin E-p27 association in the cyclin E-overexpressing cells. These data confirm that cyclin D1 ... The potential roles of cyclin D1 and cyclin E expression as markers of therapeutic responsiveness to the pure steroidal ...

https://www.garvan.org.au/research/publications/1565

Effect of betulinic acid on the regulation of Hiwi and cyclin B1 in human gastric adenocarcinoma AGS cells ... Effect of betulinic acid on the regulation of Hiwi and cyclin B1 in human gastric adenocarcinoma AGS cells Li-jing Yang, Yan ... The expression of Hiwi and Cyclin B1 was down-regulated in BA-treated AGS cells in a dose-dependent manner.. Conclusion: BA ... Both FCM and reverse transcription-PCR (RT-PCR) technologies were applied to detect the expression of Hiwi and Cyclin B1.. ...

http://www.chinaphar.com/article/view/4509

Saccharomyces Cerevisiae G1 Cyclins Are Differentially Involved in Invasive and Pseudohyphal Growth Independent of the ... Saccharomyces Cerevisiae G1 Cyclins Are Differentially Involved in Invasive and Pseudohyphal Growth Independent of the ... Resources relating to Saccharomyces Cerevisiae G1 Cyclins Are Differentially Involved in Invasive and Pseudohyphal Growth ...

https://www.dechert.com/knowledge/publication/1999/1/saccharomyces-cerevisiae-g1-cyclins-are-differentially-involved.html

... those synthesizing the delayed response genes and cyclin D/Cdk4 in response to growth signals; (c) the E2F-dependent cyclin E/ ... those synthesizing the delayed response genes and cyclin D/Cdk4 in response to growth signals; (c) the E2F-dependent cyclin E/ ... those synthesizing the delayed response genes and cyclin D/Cdk4 in response to growth signals; (c) the E2F-dependent cyclin E/ ... those synthesizing the delayed response genes and cyclin D/Cdk4 in response to growth signals; (c) the E2F-dependent cyclin E/ ...

https://research.vu.nl/en/publications/restriction-point-control-of-the-mammalian-cell-cycle-via-the-cyc

Cks proteins have been implicated in entry into and exit from mitosis, by promoting Cyclin-dependent kinase (Cdk) activity on ... Cks30A mutants are compromised for Cyclin A destruction, resulting in an arrest or delay at the metaphase/anaphase transition, ... Cks30A appears to regulate Cyclin A levels through the activity of a female germline-specific anaphase-promoting complex, CDC20 ... Cks proteins have been implicated in entry into and exit from mitosis, by promoting Cyclin-dependent kinase (Cdk) activity on ...

https://scholar.uwindsor.ca/biologypub/1063/

The indirubins are known inhibitors of cyclin dependent kinases (CDKs), including CRK3 from the protozoan parasite Leishmania ... Indirubin inhibitors of Leishmania mexicana CRK3 Cyclin Dependent Kinase Poster Published: March 7, 2007 ... The indirubins are known inhibitors of cyclin dependent kinases (CDKs), including CRK3 from the protozoan parasite Leishmania ... and 5-positions exhibit significant selectivity as inhibitors of CRK3 compared with CDK1/cyclin B in vitro. ...

https://www.technologynetworks.com/tn/posters/indirubin-inhibitors-of-leishmania-mexicana-crk3-cyclin-dependent-kinase-230511

The p21WAF1 cyclin-dependent kinase inhibitor plays a key role in senescence and in cell cycle arrest after DNA damage. Here, ... Tbx2 Directly Represses the Expression of the p21WAF1 Cyclin-Dependent Kinase Inhibitor Share Share Share ... The p21WAF1 cyclin-dependent kinase inhibitor plays a key role in senescence and in cell cycle arrest after DNA damage. Here, ... Tbx2 Directly Represses the Expression of the p21WAF1 Cyclin-Dependent Kinase Inhibitor ...

https://www.ndm.ox.ac.uk/publications/92996

Progression of cell cycle is regulated by sequential expression of cyclins, which associate with distinct cyclin kinases to drive the transition between different cell cycle phases. (rcsb.org)
Cyclin-dependent kinases (CDKs) are enzymes that play an important role in cell division, making them attractive therapeutic targets for certain cancers, including some types of breast cancer. (fda.gov)
The activation of cyclin-dependent protein kinases (Cdks) is dependent upon site-specific phosphorylation and dephosphorylation reactions, as well as positive and negative regulatory subunits. (semanticscholar.org)
Cyclin-dependent kinases: initial approaches to exploit a novel therapeutic target. (semanticscholar.org)
Cyclins serve as regulators of CDK kinases. (neuromics.com)
Cyclin B2 (CCNB2) is a member of cyclin family proteins, which regulate the activities of cyclin dependent kinases (CDKs) and different cyclins function spatially and temporally in specific phases of the cell cycle. (ptglab.com)
The indirubins are known inhibitors of cyclin dependent kinases (CDKs), including CRK3 from the protozoan parasite Leishmania mexicana, which is essential for proliferation of the disease Leishmaniasis. (technologynetworks.com)
Cyclin F, unlike canonical and transcriptional cyclins, does not bind or activate any cyclin-dependent kinases. (aging-us.com)
Orderly progression through the cell cycle is driven by the periodic oscillations in the activity of cyclin-dependent kinases (CDKs) [ 1 ]. (aging-us.com)
The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. (fishersci.no)
Cell cycle progression is regulated by cyclin-dependent protein kinases at both the G1/S and the G2/M transitions. (reactome.org)
In light of investigations documenting a central role for cyclin-dependent kinases in controlling the cell cycle, we addressed the hypothesis that P. carinii inhibits epithelial cell growth by interfering with host epithelial cyclin-dependent kinase (cdk) activity. (elsevier.com)
In this paper, we show that substrate specificity is primarily conferred on human mitotic cyclin-dependent kinases (CDKs) by their subcellular localization. (elsevier.com)
Cyclin-dependent kinases 4 and 6 (CDK4/6) are key drivers from the cell routine and are necessary for the initiation and development of varied malignancies1,2. (woofahs.com)
Binding of cyclin D1 to its kinase partners, the cyclin dependent kinases 4 and 6 (CDK4\6) results in the formation of active complexes that phosphorylate the Retinoblastoma tumor suppressor protein (RB). (biomedcentral.com)
A large family of regulatory proteins that function as accessory subunits to a variety of CYCLIN-DEPENDENT KINASES. (bvsalud.org)
Cyclin-dependent kinases. (who.int)
PFTAIRE-2 shares sequence similarity with Cyclin-Dependent Kinases (CDKs), which belong to a large family of STKs that are regulated by their cognate cyclins. (umbc.edu)

The activity of CDKs, in turn, is controlled by their binding to allosteric regulatory proteins called cyclins. (aging-us.com)
However, unlike cyclin A and many other cyclins, cyclin F does not bind or activate CDKs [ 3 ]. (aging-us.com)
The difference in localization of the B-type cyclin-CDKs underlies the ability of cyclin B1-CDK1 to cause chromosome condensation, reorganization of the microtubules, and disassembly of the nuclear lamina and of the Golgi apparatus, while it restricts cyclin B2-CDK1 to disassembly of the Golgi apparatus. (elsevier.com)
Together, CDKs and cyclins are involved in the control of cell-cycle progression, transcription, and neuronal function. (umbc.edu)

CDER researchers analyzed data from patients with hormone receptor-positive, human epidermal growth factor receptor 2-negative advanced or metastatic breast cancer to determine whether any patient or tumor characteristics were associated with more (or less) benefit from adding a cyclin-dependent kinase inhibitor to a hormonal agent. (fda.gov)
All patient subgroups benefited from the addition of a cyclin-dependent kinase inhibitor to hormonal therapy. (fda.gov)
Addgene: Suppression of cell transformation by the cyclin-dependent kinase inhibitor p57KIP2 requires binding to proliferating cell nuclear antigen. (addgene.org)
The p21WAF1 cyclin-dependent kinase inhibitor plays a key role in senescence and in cell cycle arrest after DNA damage. (ox.ac.uk)

Using cell-free extracts from Xenopus eggs, we have investigated the mechanisms underlying the inability of a recombinant Cdc2/cyclin B complex to induce mitosis in the presence of incompletely replicated DNA. (caltech.edu)
However, at threshold concentrations, a Cdc2/cyclin B complex containing a mutant Cdc2 subunit that cannot be phosphorylated on either tyrosine 15 or threonine 14 displays a markedly reduced capacity to induce mitosis in the presence of aphidicolin. (caltech.edu)
Cks proteins have been implicated in entry into and exit from mitosis, by promoting Cyclin-dependent kinase (Cdk) activity on mitotic substrates. (uwindsor.ca)
2007). Cyclin B1 is primarily cytoplasmic during interphase and translocates into the nucleus at the onset of mitosis (Jackman et al. (reactome.org)
1999). Cyclin B2 colocalizes with the Golgi apparatus and contributes to its fragmentation during mitosis (Jackman et al. (reactome.org)
The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. (anticorps-enligne.fr)
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. (bvsalud.org)
Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes , but their specialised functions in mitosis are incompletely understood. (bvsalud.org)
Cells lacking cyclin B can enter mitosis and phosphorylate most mitotic proteins , because of parallel PP2AB55 phosphatase inactivation by Greatwall kinase . (bvsalud.org)

In ICI 182780-treated cyclin D1-overexpressing cells, sufficient Cdk activity was retained to allow retinoblastoma protein phosphorylation and cell proliferation, despite an increase in the association of p21 and p27 with cyclin D1-Cdk4/6 and cyclin E-Cdk2 complexes. (garvan.org.au)
Furthermore, evaluation of The Cancers Genome Atlas (TCGA) data7 uncovered that breasts malignancies harboring cyclin D1 amplification (i.e., improved CDK4/6 activity) screen significantly lower appearance of and than non-amplified tumors (Prolonged Data Fig. 2d). (woofahs.com)
This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. (anticorps-enligne.fr)
Kisqali is a cyclin-dependent kinase 4/6 (CDK4/6) inhibitor. (vsyl.net)

Various cyclins demonstrate distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. (neuromics.com)
Furthermore, we show that the disassembly of the Golgi apparatus initiated by either mitotic cyclin-CDK complex does not require mitogen-activated protein kinase kinase (MEK) activity. (elsevier.com)
Shape 2 LMW-E overexpression results in mitotic defects Cyclin E expression cooperates with p53 loss in causing mitotic defects and chromosome missegregation Presence of the tumor suppressor p53 is known to Clofibrate be a crucial component of a checkpoint that limits the accumulation of cells with supernumerary centrosomes (24). (immune-source.com)
To examine whether p53 loss cooperates with cyclin E overexpression (EL or LMW-E) to induce mitotic defects we introduced EL and LMW-E by adenoviral contamination into human mammary epithelial 76NF2V and 76NE6 cells (Fig. 3A). (immune-source.com)
Loss of cyclin A in G2-phase prevents mitotic entry. (bvsalud.org)
The final barrier to mitotic establishment corresponds to nuclear envelope breakdown, which requires a decisive shift in the balance of cyclin-dependent kinase Cdk1 and PP2AB55 activity. (bvsalud.org)
Beyond this point, cyclin B /Cdk1 is essential for phosphorylation of a distinct subset of mitotic Cdk1 substrates that are essential to complete cell division . (bvsalud.org)
Our results identify how cyclin A , cyclin B and Greatwall kinase coordinate mitotic progression by increasing levels of Cdk1-dependent substrate phosphorylation . (bvsalud.org)

Our findings link HTLV-I infection to changes in cellular D-type cyclin gene expression, transformation of T cells and subsequent development of T-cell leukemia. (mdc-berlin.de)
The cyclin D1 gene product encodes the regulatory subunit of the holoenzyme that phosphorylates pRB and NRF1 thereby governing cell-cycle progression and mitochondrial metabolism. (jefferson.edu)
have now examined how the activities of β-catenin and the cyclin D1 gene change in living human cells. (elifesciences.org)
These analyses were initially performed in a population of cells, and confirmed that β-catenin rapidly accumulates after a Wnt signal and that the cyclin D1 gene becomes activated. (elifesciences.org)
The protein encoded by this gene is a member of the cyclin family and contains the cyclin box. (fishersci.no)
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (anticorps-enligne.fr)
The CCND2 gene provides instructions for making a protein called cyclin D2. (medlineplus.gov)
The molecular consequence of translocation is overexpression of the protein cyclin D1 (coded by the PRAD1 gene located close to the breakpoint). (medscape.com)
2008. Rac-dependent cyclin D1 gene expression regulated by cadherin- and integrin-mediated adhesion. . (ucsf.edu)

Endogenous cyclin D1 was required for the recruitment of G9a to target genes in chromatin, for G9a-induced H3K9me2 of histones, and for NL-LAD interaction. (jefferson.edu)
The finding that cyclin D1 is required for recruitment of G9a to target genes in chromatin and for H3K9 dimethylation, identifies a novel mechanism coordinating protein methylation. (jefferson.edu)
One of these genes, called cyclin D1 , controls cell division. (elifesciences.org)
Experimental Design: To test this hypothesis, we used ovarian cancer effusion as an example and applied a quantitative real-time PCR tomeasure the relative copy number and strand length of DNA fragments from one of the most frequently amplified genes, cyclin E, in ovarian serous carcinomas. (elsevier.com)

It has been shown that the direct interaction between the Cyclin A-CDK2 complex and origin recognition complex subunit 1 (ORC1) mediates the localization of ORC1 to centrosomes, where ORC1 inhibits cyclin E-mediated centrosome reduplication. (rcsb.org)
Here we report the crystal structure of Cyclin A-CDK2 complex bound to a peptide derived from ORC1 at 2.54 å resolution. (rcsb.org)
This was associated with a fall in cyclin D1 levels, a reduction in the half-life of cyclin D1 protein and a decline in cyclin E-Cdk2 activity in cyclin D1-overexpressing cells, and the maintenance of cyclin E-p27 association in the cyclin E-overexpressing cells. (garvan.org.au)
Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex. (vu.nl)
Dive into the research topics of 'Restriction point control of the mammalian cell cycle via the cyclin E/Cdk2:p27 complex. (vu.nl)
Sensitivity and co-control analysis indicated that the strongest control of the RP is mediated via the cyclin E/Cdk2:p27 complex concentration. (vu.nl)
apparent effects of other molecular species were indirect and always worked through cyclin E/Cdk2:p27, indicating a causal relationship between this complex and the positioning of the RP. (vu.nl)
A second set (p27, cyclin B and cdk2) are initially expressed at low levels but ascend to peak levels only to decline again. (elsevier.com)
In phase 1 cyclin E and p21 levels of mRNA expression are high, while those of mRNAs of p27, cdk2 and cyclin B are low. (elsevier.com)
In the study, inhibiting cyclin-dependent kinase 2 (CDK2) was found to protect rodents from noise and drug related hearing loss by preventing the death of inner ear cells. (ctnursinghomes.com)

This protein and neuron-specific CDK5 activator CDK5R1/p39NCK5A both share limited similarity to cyclins, and thus may define a distinct family of cyclin-dependent kinase activating proteins. (nih.gov)
Instead, it harbors an F-box motif and primarily functions as the substrate recognition subunit of the Skp1-Cul1-F-box E3 ubiquitin ligase complex, SCF Cyclin F . By targeting specific proteins for ubiquitin-mediated proteasomal degradation, cyclin F plays a critical role in the regulation of centrosomal duplication, DNA replication and repair, and maintenance of genomic stability. (aging-us.com)
Instead, cyclin F is the founding member of the F-box family of proteins, whose 69 members share a conserved F-box domain [ 4 ]. (aging-us.com)
Within this complex, cyclin F functions as the substrate-recognition subunit and targets specific proteins for ubiquitylation, and subsequent degradation [ 5 ]. (aging-us.com)
In phase 2 levels of expression of cyclin E and p21 fall to asymptote while levels of expression of mRNA of the other three proteins reach their peaks. (elsevier.com)
In phase 3 levels of expression of cyclin E and p21 mRNAs remain low and those of the mRNAs of the other three proteins fall. (elsevier.com)
Cyclins are a family of proteins that control how cells proceed through the multi-step cycle of cell division. (medlineplus.gov)

Red: PE-labeled Cyclin D1 Monoclonal Antibody (SPM587). (novusbio.com)

Correlating β-catenin nuclear dynamics to cyclin D1 transcriptional activation showed that the nuclear accumulation rate of change of the signaling factor, and not actual protein levels, correlated with the transcriptional output of the pathway. (elifesciences.org)
A subset of cyclins may also function as transcriptional regulators. (bvsalud.org)

The nuclear export of cyclin D1 has been shown to require prior phosphorylation on Thr-286 by glycogen synthase kinase 3β (GSK3β) [ 3 ]. (biomedcentral.com)
This phosphorylation of cyclin D1 was initially thought to regulate its ubiquitin-dependent degradation. (biomedcentral.com)
Nevertheless, it is still generally believed that cyclin D1 accumulates within the nucleus during G1, and at the G1-S-phase transition, GSK3β accumulates in the nucleus and mediates phosphorylation, nuclear export and subsequent ubiquitin-dependent degradation of cyclin D1 in the cytoplasm. (biomedcentral.com)
Vanadate also increased p21 and Chk1 levels and reduced Cdc25C expression, leading to phosphorylation of Cdc2 and a slight increase in cyclin B1 expression as analyzed by Western blot. (cdc.gov)
Catalase, a specific antioxidant for H2O2, decreased vanadate-induced expression of p21 and Chk1, reduced phosphorylation of Cdc2Tyr15, and decreased cyclin B1 levels. (cdc.gov)
Several regulatory pathways are involved: (1) activation of p21, (2) an increase of Chk1 expression and inhibition of Cdc25C, which results in phosphorylation of Cdc2 and possible inactivation of cyclin B1/Cdc2 complex. (cdc.gov)
2005. Phosphorylation of spinophilin by ERK and cyclin-dependent PK 5 (Cdk5). . (cornell.edu)

We provide evidence that the checkpoint-dependent suppression of the Cdc2/cyclin B complex involves a titratable inhibitor that is regulated by the presence of unreplicated DNA. (caltech.edu)

Our data suggest that induction of cyclins D1 and D2 by Tax is involved in IL-2-independent cell-cycle progression as well as IL-2-independent transformation of primary human T cells by HTLV-I. High expression levels of cyclin D1 and D2 mRNAs were also detected in some patients with ATL. (mdc-berlin.de)
Cyclin B2 serves a key role in progression of G2/M transition. (ptglab.com)
Among the cyclins that play a crucial role in cell-cycle progression, is cyclin F. It is most similar to cyclin A, both in terms of amino acid sequence and the cyclic pattern of expression during the cell cycle [ 3 ]. (aging-us.com)
During cell cycle progression, protein levels of the cyclin begin to rise early in G1, prior to its rapid nuclear export and degradation within the cytoplasm. (biomedcentral.com)

nevertheless the level to which these tumor-specific forms trigger genomic instability differs from that of full-length cyclin E (Un) as well as the root mechanism(s) have however to become elucidated. (immune-source.com)

We identify the region of cyclin B2 responsible for its localization and show that this will direct cyclin B1 to the Golgi apparatus and confer upon it the more limited properties of cyclin B2. (elsevier.com)
Inhibition of GSK3β or CRM1-dependent nuclear export resulted in only modest nuclear accumulation, suggesting that the cytoplasmic localization of cyclin D1 results from the inhibition of its nuclear import. (biomedcentral.com)
In these cells, the enforced nuclear localization of cyclin D1 induced apoptosis. (biomedcentral.com)
The subcellular localization of cyclin D1 may thus play a role in regulating cellular survival. (biomedcentral.com)

Assignment of CDK5R2 coding for the cyclin-dependent kinase 5, regulatory subunit 2 (NCK5AI protein) to human chromosome band 2q35 by fluorescent in situ hybridization. (nih.gov)

It is not understood however, how cells integrate the CDK-dependent and independent activities of cyclin D1. (biomedcentral.com)

Drosophila Cks30A interacts with Cdk1 to target Cyclin A for destructi" by Andrew Swan, Gail Barcelo et al. (uwindsor.ca)
In our hands, indirubins substituted at both the 3' and 5-positions exhibit significant selectivity as inhibitors of CRK3 compared with CDK1/cyclin B in vitro. (technologynetworks.com)
The two B-type cyclins localize to different regions within the cell and are thought to have specific roles as CDK1-activating subunits (see Bellanger et al. (reactome.org)

Our aim was to examine the involvement of G1 cell-cycle regulators in cell growth dysregulation induced by HTLV-I. Compared to uninfected cells, higher expression levels of cyclin D1 and D2 mRNA were detected in HTLV-I-infected T-cell lines, which were at least in part mediated by the viral transforming protein Tax since Tax activated both cyclin D1 and D2 promoters in the human T-cell line Jurkat. (mdc-berlin.de)
We have measured the levels of cyclin mRNAs and polypeptides during oogenesis, progesterone-induced oocyte maturation, and immediately after egg activation in the frog, Xenopus laevis. (rupress.org)

Observations and the sequence similarity to the kinase/cyclin pair Srb10/Srb11 in S. cerevisiae suggest that cyclin C and Cdk8 control RNA polymerase II function. (semanticscholar.org)
These data confirm that cyclin D1 expression and cyclin E-p27 association play important roles in antiestrogen action, and suggest that cyclin D1 or cyclin E overexpression has subtle effects on antiestrogen sensitivity. (garvan.org.au)

Epstein-Barr virus latent membrane protein expression and cyclin DI cell cyase protein expression in malignant and normal oesophageal tissues to see whether any variation in their expression in these tissues could be of diagnostic or prognostic value. (bvsalud.org)
EBV-LMPI protein expression and cyclin DI expression were studied immunohisto chemically in these tissue sections. (bvsalud.org)

Sharma SS , Pledger WJ , Kondaiah P , . The deubiquitylase USP7 is a novel cyclin F-interacting protein and regulates cyclin F protein stability. (aging-us.com)

This study provides a structural basis of the specific ORC1-cyclins recognition, with implication in development of novel inhibitors against the cyclin/CDK complexes. (rcsb.org)

It is shown that cyclin H and Cdk7 are present and during meiosis, form active complexes in testicular cells and are strong candidates for the activating kinase for cyclin A1-associated kinase. (semanticscholar.org)

Furthermore, miR‑30a overexpression upregulates BCL2A1, IER3 and cyclin D2 expression by inhibiting FOXL2. (spandidos-publications.com)
Overexpression of cyclin E produced a less pronounced early cell cycle effect indicating only partial resistance to antiestrogen inhibition in the short-term. (garvan.org.au)
Cell viability and colony formation assays revealed that overexpression of miR-34a in BC cells enhances the chemosensitivity of cisplatin, doxorubicin, epirubicin and mitomycin C. Furthermore, miR-34a inhibited cell proliferation and triggered G0/G1 cell cycle arrest by inhibiting cyclin D1 and cyclin E2 protein expression. (bvsalud.org)

Pharmacological inhibition of the deubiquitylase activity of USP7 resulted in downregulation of cyclin F mRNA. (aging-us.com)
0.01) downregulation of p53, Ki67, and cyclin D1 expression at 3 months as compared to baseline in the combination group. (kanker-actueel.nl)

Cks30A appears to regulate Cyclin A levels through the activity of a female germline-specific anaphase-promoting complex, CDC20-Cortex. (uwindsor.ca)
Cyclin F functionally interacts with these substrates and interaction partners to chiefly regulate genomic and chromosomal stability. (aging-us.com)
Our results suggest that cytoplasmic sequestration may additionally serve to regulate cyclin D1 activity in mammalian cancer cells. (biomedcentral.com)
Mirk activity is restricted to the G0-/early G1-phase of the cell cycle and may not regulate cyclin D1 in actively cycling cells [ 6 ]. (biomedcentral.com)
Cyclin D2 helps to regulate a step in the cycle called the G1-S transition, in which the cell moves from the G1 phase, when cell growth occurs, to the S phase, when the cell's DNA is copied (replicated) in preparation for cell division. (medlineplus.gov)

It is indicated for seizures associated with cyclin-dependent kinase-like 5 (CDKL5) deficiency disorder (CDD) in patients aged 2 years and older. (medscape.com)

Flow Cytometry: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Flow Cytometric analysis of human Cyclin D1 on MCF-7 cells. (novusbio.com)
Flow Cytometry: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Flow Cytometric analysis of human Cyclin D1 on Jurkat cells. (novusbio.com)

P-TEFb-associated cyclin-dependent protein. (wikigenes.org)
The cyclin-dependent kinase inhibitory domain of the yeast Sic1 protein is contained within the C-terminal 70 amino acids. (wikigenes.org)
Molecular cloning of CDK7-associated human MAT1, a cyclin-dependent kinase-activating kinase (CAK) assembly factor. (semanticscholar.org)
CyclinA2-Cyclin-dependent Kinase Regulates SAMHD1 Protein Phosphohydrolase Domain. (stowers.org)
The expression of Hiwi and Cyclin B1 was down-regulated in BA-treated AGS cells in a dose-dependent manner. (chinaphar.com)
The inhibitory effect on cyclin-dependent kinase activity was mediated by the trophozoite form of P. carinii, in that highly purified trophozoites exerted marked inhibition of p34(cdc2) activity. (elsevier.com)
Cyclin-dependent kinase 5 is associated with risk for Alzheimer's disease in a Dutch population-based study. (ox.ac.uk)
We have demonstrated previously, that TSA induces the ubiquitin-dependent degradation of cyclin D1 in MCF-7 breast cancer cells. (biomedcentral.com)
A new drug that that inhibits an enzyme known as cyclin-dependent kinase 2 could potentially save the hearing of millions of people. (ctnursinghomes.com)
Ashok B. Kulkarni, Ph.D., has spent years studying a protein called cyclin-dependent kinase 5 (Cdk5). (medlineplus.gov)

Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) Rabbit mAb. (cellsignal.com)
Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587). (novusbio.com)
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - analysis of Cyclin D1 in human breast cancer cell line MDA-MB-231 cell lysate using anti-Cyclin D1. (novusbio.com)
Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. (novusbio.com)
This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). (novusbio.com)
Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1. (novusbio.com)
CCND2 is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (neuromics.com)
The aberrant expression of Cyclin B2 deregulates spindle checkpoints in the cell cycle and results in chromosomal instability (CIN), one of the signature phenotypes of most cancers. (ptglab.com)
This role in cell cycle control is mediated by meis1 regulating cyclin D1 and c-myc transcription. (biologists.com)
BA exerted potent effect on growth inhibition, G 2 /M cell cycle arrest and induction of apoptosis in AGS cells in vitro , possibly associated with the down-regulation of Hiwi and its downstream target Cyclin B1 expression. (chinaphar.com)
Cyclin F abundance and activity are tightly regulated throughout the cell cycle. (aging-us.com)
Further studies revealed that the compounds were able to induce cancer cell differentiation and concomitantly downregulate cyclin D1 expression with upregulation of p27 levels, consistent with cell cycle arrest at the G1 phase. (strath.ac.uk)
The cyclin D1 proto-oncogene is an important regulator of G1 to S-phase transition and an important cofactor for several transcription factors in numerous cell types. (biomedcentral.com)
Our studies revealed cyclin D1 to be localized predominantly within the cytoplasmic fraction of all cell lines tested. (biomedcentral.com)
We have shown by several different experimental approaches, that cyclin D1 is in fact a predominantly cytoplasmic protein in mammalian cancer cell lines. (biomedcentral.com)
Cyclin D1 is a protein playing important role during the G1→S phase transition in the cell cycle. (who.int)
Cyclin D2's role in the cell division cycle makes it a key controller of the rate of cell growth and division (proliferation) in the body. (medlineplus.gov)
The resulting buildup of cyclin D2 in cells triggers them to continue dividing when they otherwise would not have, leading to abnormal cell proliferation. (medlineplus.gov)
It is less clear how a buildup of cyclin D2 contributes to polydactyly, although the extra digits are probably related to abnormal cell proliferation in the developing hands and feet. (medlineplus.gov)

Cks30A mutants are compromised for Cyclin A destruction, resulting in an arrest or delay at the metaphase/anaphase transition, both in female meiosis and in the early syncytial embryo. (uwindsor.ca)
The character of these phases can be understood in part as consequences of the reciprocal regulatory influence of p27 and cyclin E and of the rate limiting functions of p27 at the restriction point and of cyclin E at the G1 to S transition. (elsevier.com)

abstract = "Background Mutation in S-phase cyclin A-associated protein rin the endoplasmic reticulum (SCAPER) have been found across ethnicities and have been shown to cause variable penetrance of an array of pathological traits, including intellectual disability, retinitis pigmentosa and ciliopathies. (bgu.ac.il)

Additionally, our data suggest that USP7 is also involved in the regulation of cyclin F mRNA. (aging-us.com)
Additional studies were initiated in order to further investigate the effect of TSA on cyclin D1 regulation using sub-cellular fractionation techniques. (biomedcentral.com)

However, the molecular basis underlying the specific recognition between ORC1 and cyclins remains elusive. (rcsb.org)
However, the molecular mechanisms regulating cyclin F are scantily understood. (aging-us.com)

Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - HELLS expression and protein levels are modulated with YAP1/TEAD inhibition downstream of SHH signaling. (novusbio.com)

More recently, the serine/threonine kinase Mirk/Dyrk1B was shown to enhance cyclin D1 degradation by phosphorylating Thr288. (biomedcentral.com)

Moreover, serum circulating Cyclin B2 mRNA expression has been found increased in cancer patients and associated with cancer stage and metastasis status. (ptglab.com)

Using its F-box, cyclin F interacts with Skp1, which simultaneously recruits Cul1 (and RBX1 with Cul1): together they assemble into a functional SCF Cyclin F (Skp1-Cul1-F box) E3 ubiquitin ligase complex [ 4 , 5 ]. (aging-us.com)
We evaluated and compared the expression of Cyclin D1 in 56 endometrial samples including 24 cases of simple hyperplasia, 12 cases of complex hyperplasia and 10 cases each of secretory and proliferative endometrium.Results: A substantial increase in expression of Cyclin D1 was seen in hyperplastic endometrium compared to normal endometrium. (who.int)
Moreover, complex hyperplasia showed the maximum positivity for Cyclin D1.Conclusions: Cyclin D1 may play a stimulatory role in the proliferation of endometrial glands and hence may be involved in endometrial tumorigenesis. (who.int)

The disease is confirmed by immunohistochemistry staining for CD20, CD5, and cyclin D1. (medscape.com)

De novo CCND2 mutations leading to stabilization of cyclin D2 cause megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome. (medlineplus.gov)

q32) translocation resulting in over-expression of cyclin D1. (novusbio.com)

The structure revealed that the ORC1 peptide interacts with a hydrophobic groove, termed cyclin binding groove (CBG), of Cyclin A via a KXL motif. (rcsb.org)

Histopathological examination indicated the presence of a moderate epithelial dysplasia while immunohistochemistry for Ki-67 and cyclin D1 revealed the presence of cellular proliferation in suprabasal layers. (bvsalud.org)
Immunomarking with Ki-67 and cyclin D1 revealed expressive presence of cellular proliferation in suprabasal layers ( Figure 3 ). (bvsalud.org)

Equally, directing cyclin B2 to the cytoplasm with the NH 2 terminus of cyclin B1 confers the broader properties of cyclin B1. (elsevier.com)

One set of mRNAs (cyclin E and p21) are initially expressed at high levels but expression then falls to a low asymptote. (elsevier.com)

Instead, cells appeared to sense how quickly the amount of β-catenin in the nucleus changes over time, and this rate influences the activation of cyclin D1 . (elifesciences.org)
7 days) treatment, antiestrogens inhibited colony growth in cyclin D1- or cyclin E-overexpressing breast cancer cells, but with an approximately 2-2.5-fold decrease in dose sensitivity. (garvan.org.au)
Recent studies have shown that the cytoplasmic sequestration of cyclin D1 prevents apoptosis in neuronal cells. (biomedcentral.com)
Tsunekawa Y, Kikkawa T, Osumi N. Asymmetric inheritance of Cyclin D2 maintains proliferative neural stem/progenitor cells: a critical event in brain development and evolution. (medlineplus.gov)

The cyclin C/Cdk8 kinase. (semanticscholar.org)

Distinct from other identified CBG-binding sequences, an arginine residue flanking the KXL motif of ORC1 inserts into a neighboring acidic pocket, contributing to the strong ORC1-Cyclin A association. (rcsb.org)

No . relation was observed between cyclin/PCNA and axillary lymphonodal envolvement, histologic and nuclear grades and estrogen receptor. (ndltd.org)
Bad prognosis was observed in cases with cyclin/PCNA grade III-IV, nuclear grade 1 and negative estrogen receptor in both negative and positive axilla cases. (ndltd.org)

This antibody neutralizes the activity of cyclin D1 in vivo. (novusbio.com)
saw that the absolute number of β-catenin molecules in the nucleus did not affect the activity of cyclin D1 . (elifesciences.org)
We observe that USP7 stabilizes cyclin F protein and that this function is independent of the deubiquitylase activity of USP7. (aging-us.com)
Studies on neonatal cardiomyocytes and postmitotic neurons indicate that the activity of cyclin D1 may be regulated through its cytoplasmic sequestration. (biomedcentral.com)

Anatomy8

Organisms1

Diseases1

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes20

Technology, Industry, Agriculture1

Information Science3

Control of cell cycle progression by c-Jun is p53 dependent. (1/114)

Altered regulation of cyclin G in human breast cancer and its specific localization at replication foci in response to DNA damage in p53+/+ cells. (2/114)

A G1 cyclin is necessary for maintenance of filamentous growth in Candida albicans. (3/114)

G1 checkpoint protein and p53 abnormalities occur in most invasive transitional cell carcinomas of the urinary bladder. (4/114)

Recovery of the yeast cell cycle from heat shock-induced G(1) arrest involves a positive regulation of G(1) cyclin expression by the S phase cyclin Clb5. (5/114)

Ectopic expression of Cdc25A accelerates the G(1)/S transition and leads to premature activation of cyclin E- and cyclin A-dependent kinases. (6/114)

A role of cyclin G in the process of apoptosis. (7/114)

Interferon-alpha inhibits proliferation in human T lymphocytes by abrogation of interleukin 2-induced changes in cell cycle-regulatory proteins. (8/114)

Cyclin G1

Cyclin G

Cyclin D1

Cyclin A

Cyclin E

Cyclin B

Cyclin B1

Cyclin D2

Cyclin D3

Cyclin A1

Cyclins

Cyclin A2

Cyclin D

Cyclin G2

Cyclin C

Cell Cycle

Cyclin-Dependent Kinases

Cyclin B2

Cyclin-Dependent Kinase 2

Cyclin T

Cyclin H

G1 Phase

Cyclin-Dependent Kinase 4

CDC2-CDC28 Kinases

CDC2 Protein Kinase

S Phase

Retinoblastoma Protein

Protein Phosphatase 2

Tumor Suppressor Protein p53

G2 Phase

Cell Cycle Proteins

Mitosis

Cyclin-Dependent Kinase Inhibitor p27

Cyclin I

Cyclin-Dependent Kinase Inhibitor p21

Phosphorylation

Cell Line, Tumor

Cell Division

NIH 3T3 Cells

Oncogene Proteins

Genes, bcl-1

Interleukin-12 Receptor beta 2 Subunit

Proto-Oncogene Proteins c-mdm2

Cell Proliferation

Cell Nucleus

Protein-Serine-Threonine Kinases

Cyclin-Dependent Kinase 6

Molecular Sequence Data

Down-Regulation

Transfection

RNA, Messenger

Adenocarcinoma, Papillary

Protein Binding

Cell Line

Proteasome Endopeptidase Complex

Gene Expression Regulation, Neoplastic

S-Phase Kinase-Associated Proteins

Tumor Cells, Cultured

Proto-Oncogene Proteins

DNA Damage

3T3 Cells

Amino Acid Sequence

Phosphoprotein Phosphatases

Nuclear Proteins

Recombinant Fusion Proteins

Base Sequence

Apoptosis

A Kinase Anchor Proteins

Gene Expression Regulation

Tumor Suppressor Proteins

Detergents

Transcriptional Activation

Transcription Factors

Up-Regulation

Fibroblasts

Enzyme Inhibitors

Retroviridae

HeLa Cells

Control of cell cycle progression by c-Jun is p53 dependent. (1/114)

Altered regulation of cyclin G in human breast cancer and its specific localization at replication foci in response to DNA damage in p53+/+ cells. (2/114)