That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
The largest of polypeptide chains comprising immunoglobulins. They contain 450 to 600 amino acid residues per chain, and have molecular weights of 51-72 kDa.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Precise and detailed plans for the study of a medical or biomedical problem and/or plans for a regimen of therapy.
Antibodies produced by a single clone of cells.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.
A cyclin B subtype that colocalizes with MICROTUBULES during INTERPHASE and is transported into the CELL NUCLEUS at the end of the G2 PHASE.
A cyclin subtype that is transported into the CELL NUCLEUS at the end of the G2 PHASE. It stimulates the G2/M phase transition by activating CDC2 PROTEIN KINASE.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Protein encoded by the bcl-1 gene which plays a critical role in regulating the cell cycle. Overexpression of cyclin D1 is the result of bcl-1 rearrangement, a t(11;14) translocation, and is implicated in various neoplasms.
The mulberry plant family of the order Urticales, subclass Hamamelidae, class Magnoliopsida. They have milky latex and small, petalless male or female flowers.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A cyclin subtype that has specificity for CDC2 PROTEIN KINASE and CYCLIN-DEPENDENT KINASE 2. It plays a role in progression of the CELL CYCLE through G1/S and G2/M phase transitions.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A cyclin B subtype that colocalizes with GOLGI APPARATUS during INTERPHASE and is transported into the CELL NUCLEUS at the end of the G2 PHASE.
Proteins prepared by recombinant DNA technology.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)
A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.

The mitogen-activated protein kinase signaling pathway stimulates mos mRNA cytoplasmic polyadenylation during Xenopus oocyte maturation. (1/760)

The Mos protein kinase is a key regulator of vertebrate oocyte maturation. Oocyte-specific Mos protein expression is subject to translational control. In the frog Xenopus, the translation of Mos protein requires the progesterone-induced polyadenylation of the maternal Mos mRNA, which is present in the oocyte cytoplasm. Both the Xenopus p42 mitogen-activated protein kinase (MAPK) and maturation-promoting factor (MPF) signaling pathways have been proposed to mediate progesterone-stimulated oocyte maturation. In this study, we have determined the relative contributions of the MAPK and MPF signaling pathways to Mos mRNA polyadenylation. We report that progesterone-induced Mos mRNA polyadenylation was attenuated in oocytes expressing the MAPK phosphatase rVH6. Moreover, inhibition of MAPK signaling blocked progesterone-induced Mos protein accumulation. Activation of the MAPK pathway by injection of RNA encoding Mos was sufficient to induce both the polyadenylation of synthetic Mos mRNA substrates and the accumulation of endogenous Mos protein in the absence of MPF signaling. Activation of MPF, by injection of cyclin B1 RNA or purified cyclin B1 protein, also induced both Mos protein accumulation and Mos mRNA polyadenylation. However, this action of MPF required MAPK activity. By contrast, the cytoplasmic polyadenylation of maternal cyclin B1 mRNA was stimulated by MPF in a MAPK-independent manner, thus revealing a differential regulation of maternal mRNA polyadenylation by the MAPK and MPF signaling pathways. We propose that MAPK-stimulated Mos mRNA cytoplasmic polyadenylation is a key component of the positive-feedback loop, which contributes to the all-or-none process of oocyte maturation.  (+info)

p53 regulates a G2 checkpoint through cyclin B1. (2/760)

The p53 tumor suppressor controls multiple cell cycle checkpoints regulating the mammalian response to DNA damage. To identify the mechanism by which p53 regulates G2, we have derived a human ovarian cell that undergoes p53-dependent G2 arrest at 32 degrees C. We have found that p53 prevents G2/M transition by decreasing intracellular levels of cyclin B1 protein and attenuating the activity of the cyclin B1 promoter. Cyclin B1 is the regulatory subunit of the cdc2 kinase and is a protein required for mitotic initiation. The ability of p53 to control mitotic initiation by regulating intracellular cyclin B1 levels suggests that the cyclin B-dependent G2 checkpoint has a role in preventing neoplastic transformation.  (+info)

Activation of integrin and ceramide signalling pathways can inhibit the mitogenic effect of insulin-like growth factor I (IGF-I) in human breast cancer cell lines. (3/760)

Cell counting, cell cycle analysis and Western immunoblotting were used to examine the effects of non-apoptotic doses of a ceramide analogue, C2, and a synthetic arginine-glycine-aspartic acid (RGD)-containing peptide, RGD, in MCF-7 and T47D cells to determine whether activation of these signalling pathways could alter the mitogenic potential of insulin-like growth factor I (IGF-I). IGF-I alone increased total cell number in both cell lines, associated with a rise in the percentage of cells in the S-phase of the cell cycle and a co-incident increase in cyclin A production. Treatments alone had no effects on cell number or cyclin A production relative to controls. C2 inhibited IGF-I-induced mitogenesis in both lines, whereas RGD was only effective in the T47D line. Despite inhibition of cell proliferation, IGF-I stimulation of cells in S-phase and of cyclin A levels were unaffected; however, an IGF-I-induced increase in cyclin B1 levels was inhibited by 30%. Low-dose induction of integrin and ceramide signalling pathways causes cells to be blocked in S-phase, thereby inhibiting the normal cycle of events associated with the IGF-I-induced mitotic signal. Activating these pathways may not only restrict tumour growth by induction of apoptosis but they may also directly inhibit IGF-I-induced cell proliferation.  (+info)

Posttranslational regulation of the retinoblastoma gene family member p107 by calpain protease. (4/760)

The retinoblastoma protein plays a critical role in regulating the G1/S transition. Less is known about the function and regulation of the homologous pocket protein p107. Here we present evidence for the posttranslational regulation of p107 by the Ca2+-activated protease calpain. Three negative growth regulators, the HMG-CoA reductase inhibitor lovastatin, the antimetabolite 5-fluorouracil, and the cyclic nucleotide dibutyryl cAMP were found to induce cell type-specific loss of p107 protein which was reversible by the calpain inhibitor leucyl-leucyl-norleucinal but not by the serine protease inhibitor phenylmethylsulfonylfluoride, caspase inhibitors, or lactacystin, a specific inhibitor of the 26S proteasome. Purified calpain induced Ca2+-dependent p107 degradation in cell lysates. Transient expression of the specific calpain inhibitor calpastatin blocked the loss of p107 protein in lovastatin-treated cells, and the half-life of p107 was markedly lengthened in lovastatian-treated cells stably transfected with a calpastatin expression vector versus cells transfected with vector alone. The data presented here demonstrate down-regulation of p107 protein in response to various antiproliferative signals, and implicate calpain in p107 posttranslational regulation.  (+info)

Alteration in p53 pathway and defect in apoptosis contribute independently to cisplatin-resistance. (5/760)

The accumulation of molecular genetic defects selected during the adaptation process in the development of cisplatin-resistance was studied using progressive cisplatin-resistant variants (L1210/DDP2, L1210/DDP5, L1210/DDP10) derived from a murine leukemia cell line (L1210/0). Of these cell lines, only the most resistant L1210/DDP10 was cross-resistant to etoposide and deficient in apoptosis induced by these two drugs, indicating that resistance to DNA-damaging agents correlates with a defect in apoptosis. This defect was tightly associated with the loss of a Ca2+/Mg2+-dependent nuclear endonuclease activity present in the less cisplatin-resistant cells. Evidence is presented that p53-dependent function (a) is lost not only in the apoptosis defective L1210/DDP10 cells, but also in the apoptosis susceptible L1210/DDP5 cells; (b) is unrelated to drug-induced cell cycle perturbations. These results suggest that deficiency in the p53 pathway and resistance to DNA-damaging agents due to a defect in apoptosis are independent events.  (+info)

Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA. (6/760)

During oocyte maturation, cyclin B1 mRNA is translationally activated by cytoplasmic polyadenylation. This process is dependent on cytoplasmic polyadenylation elements (CPEs) in the 3' untranslated region (UTR) of the mRNA. To determine whether a titratable factor might be involved in the initial translational repression (masking) of this mRNA, high levels of cyclin B1 3' UTR were injected into oocytes. While this treatment had no effect on the poly(A) tail length of endogenous cyclin B1 mRNA, it induced cyclin B1 synthesis. A mutational analysis revealed that the most efficient unmasking element in the cyclin 3' UTR was the CPE. However, other U-rich sequences that resemble the CPE in structure, but which do not bind the CPE-binding polyadenylation factor CPEB, failed to induce unmasking. When fused to the chloramphenical acetyl transferase (CAT) coding region, the cyclin B1 3' UTR inhibited CAT translation in injected oocytes. In addition, a synthetic 3' UTR containing multiple copies of the CPE also inhibited translation, and did so in a dose-dependent manner. Furthermore, efficient CPE-mediated masking required cap-dependent translation. During the normal course of progesterone-induced maturation, cytoplasmic polyadenylation was necessary for mRNA unmasking. A model to explain how cyclin B1 mRNA masking and unmasking could be regulated by the CPE is presented.  (+info)

Cyclic AMP delays G2 progression and prevents efficient accumulation of cyclin B1 proteins in mouse macrophage cells. (7/760)

In mouse macrophage cells, the increase of the intracellular cAMP level activates protein kinase A (PKA) and results in inhibition of cell cycle progression in both G1 and G2/M phases. G1 arrest is mediated by a cdk inhibitor, p27Kip1, which prevents G1 cyclin/cdk complexes from being activated in response to colony stimulating factor-1, whereas inhibition of G2/M progression has not been fully elucidated. In this report we analyzed the effect of cAMP on G2/M progression in a mouse macrophage cell line, BAC1.2F5A. Flow cytometric analysis and mitotic index measurement using both synchronized and asynchronized cells revealed that addition of cAMP-elevating agents (8-bromoadenosine 3':5'-cyclic monophosphate and 3-isobutyl-methyl-xanthine), although they did not affect S phase progression or M/G1 transition, temporarily arrested cells in G2 but eventually the cells proceeded to M phase, resulting in about 4 hours delay of G2 progression. Timing of cyclin B1/Cdc2 kinase activation was also retarded by about 4 hours, which was accompanied by inhibition of efficient accumulation of cyclin B1 proteins. Initial induction and accumulation of cyclin B1 mRNA were not hampered, but the half life of cyclin B1 proteins was significantly shorter during G2 phase in the presence of cAMP-elevating agents compared with that of the cells blocked from progressing through M phase by nocodazole. These results imply that the cAMP/PKA pathway regulates G2 phase progression by altering the stability of a crucial cell cycle regulator.  (+info)

Cytoplasmic localization of human cdc25C during interphase requires an intact 14-3-3 binding site. (8/760)

cdc25C induces mitosis by activating the cdc2-cyclin B complex. The intracellular localization of cyclin B1 is regulated in a cell cycle-specific manner, and its entry into the nucleus may be required for the initiation of mitosis. To determine the cellular localization of cdc25C, monoclonal antibodies specific for cdc25C were developed and used to demonstrate that in human cells, cdc25C is retained in the cytoplasm during interphase. A deletion analysis identified a 58-amino-acid region (amino acids 201 to 258) in cdc25C that was required for the cytoplasmic localization of cdc25C. This region contained a specific binding site for 14-3-3 proteins, and mutations in cdc25C that disrupted 14-3-3 binding also disrupted the cytoplasmic localization of cdc25C during interphase. cdc25C proteins that do not contain a binding site for 14-3-3 proteins showed a pancellular localization and an increased ability to induce premature chromosome condensation. The cytoplasmic localization of cdc25C was not altered by gamma irradiation or treatment with the nuclear export inhibitor leptomycin B. These results suggest that 14-3-3 proteins may negatively regulate cdc25C function by sequestering cdc25C in the cytoplasm.  (+info)

TY - JOUR. T1 - Cyclin B in fish oocytes. T2 - Its cDNA and amino acid sequences, appearance during maturation, and induction of p34cdc2 activation. AU - Hirai, T.. AU - Yamashita, M.. AU - Yoshikuni, M.. AU - Lou, Y. ‐H. AU - Nagahama, Y.. PY - 1992/10. Y1 - 1992/10. N2 - Under the influence of maturation‐inducing hormone (MIH) secreted from follicle cells, oocyte maturation is finally triggered by maturation‐promoting factor (MPF), which consists of a homolog of the cdc2+ gene product of fission yeast (p34cdc2) and cyclin B. Two species of cyclin B clones were isolated from a cDNA library constructed from mature goldfish oocytes. Sequence comparisons revealed that these two clones are highly homologous (95%) and were found to be similar to Xenopus cyclin B1. Using monocional antibodies against Escherichia coli produced goldfish cyclin B and the PSTAIR sequence of p34cdc2, we examined the levels of cyclin B and p34cdc2 proteins during goldfish oocyte maturation induced in vitro by ...
In the present study, we identified three proteins (cyclin B1, TfR1, and fibronectin) that were highly expressed in metastatic ACC in the TCPA database. With a median follow-up of 3.1 years, high expression of each of these three proteins was associated with a poor survival rate. Subjects with high expression levels of a combination of two DEPs were at a higher risk for mortality than those with a high expression levels of only one DEP. Cyclin B1, TfR1, and all combinations of the three DEPs showed meaningful prognostic performance independent of age and staging. Moreover, among non-metastatic patients, combinations of these three DEPs showed significant or near-significant associations with mortality. The reason for the non-significance of fibronectin alone needs to be elucidated, but the small number of patients may have contributed to this finding. In addition, the C-index and cfNRI values of high cyclin B1/high TfR1/high fibronectin were the same as those of high cyclin B1/high fibronectin. ...
ViraQuest Inc. , Uncategorized , Estrogen receptor ? causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2 ...
10 products from 6 suppliers. Compare and order Cyclin B1 ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. Recommended products for the most popular species. Our scientists will help you find the right ELISA kit for your needs.
Achetez les anticorps cyclin b1 de Santa Cruz Biotechnology, Inc. Des anticorps monoclonaux sont disponibles pour la plupart des immunogènes protéiques.
Wee1 acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. The activity of wee1 increases during the S and G2 phases, and decreases in M phase when it is hyperphosphorylated. A correlated decrease in wee1 protein level occurs at M/G1 phase, probably due to its degradation. Wee1 specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. The phosphorylation of cyclin B1-CDK1 occurs exclusively on Tyr-15 and phosphorylation of monomeric CDK1 does not occur ...
CCNB1 Human Recombinant produced in E. coli is a single polypeptide chain containing 457 amino acids (1-433) and having a molecular mass of 50.9 kDa.
CCNB2 Human Recombinant produced E. coli is a single polypeptide chain containing 422 amino acids (1-398) and having a molecular mass of 47.9 kDa.
Complete information for CCNB2 gene (Protein Coding), Cyclin B2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
NU6102, CDK1/cyclin B and CDK2/cyclin A3 inhibitor (CAS 444722-95-6), with |98% purity. Join researchers using our high quality biochemicals.
We investigated the occurrence of transcription during mitosis on an RNA pol II‐transcribed gene. We have found that the human cyclin B1 gene is actively transcribed at the mitotic stage. This result is surprising, since it is widely accepted that transcription is repressed during mitosis in higher eukaryotes. Interestingly, in fission yeast the rate of RNA synthesis is maintained during passage through mitosis (Baum et al., 1998). In mammalian cells, until now, no RNA pol II‐dependent transcription has been reported in mitotic cells, although there is evidence showing that 10-20% of the TFIID population remains associated with the condensed mitotic chromatin (Segil et al., 1996). Whether the transcription of the cyclin B1 gene occurs during all the four mitosis phases remains to be elucidated. The cyclin B1 protein is quickly degraded at the metaphase. Whenever a spindle checkpoint is imposed during metaphase, there is a reappearance of cyclin B1 protein due to a loss of cyclin B1 ...
Cyclin B1 (G2- & M-phase Cyclin) Antibody - Without BSA and Azide, Mouse Monoclonal Antibody [Clone V92.1 ] validated in IF, FC, IP (AH10827-100), Abgent
Manni I., Tunici P., Cirenei N., Albarosa R., Colombo B.M., Roz L., Sacchi A., Piaggio G., Finocchiaro G.. Mxi1 is a Mad family member that plays a role in cell proliferation and differentiation. To test the role of Mxi1 on tumorigenesis of glioma cells we transfected a CMV-driven MXI1 cDNA in U87 human glioblastoma cells. Two clones were isolated expressing MXI1 levels 18- and 3.5-fold higher than wild-type U87 cells (clone U87.Mxi1.14 and U87.Mxi1.22, respectively). In vivo, U87.Mxi1.14 cells were not tumorigenic in nude mice and delayed development of tumours was observed with U87.Mxi1.22 cells. In vitro, the proliferation rate was partially and strongly inhibited in U87.Mxi1.22 and U87.Mxi1.14 cells respectively. The cell cycle analysis revealed a relevant accumulation of U87.Mxi1.14 cells in the G(2)/M phase. Interestingly, the expression of cyclin B1 was inhibited to about 60% in U87.Mxi1.14 cells. This inhibition occurs at the transcriptional level and depends, at least in part, on the ...
Abstract. γ-Radiation is a potent inducer of apoptosis. There are multiple pathways regulating DNA damage-induced apoptosis, and we set out to identify novel me
Metafase di anafase transizione viene attivato attraverso la promozione anafase-complesso (APC / C)-dipendente ubiquitinazione e la...
Cell Cycle, Genes, Tumor, Cell Cycle Genes, Human, Repression, Gene, Dream, Proteins, Regulation, Cell, Cyclin, Cyclin B2, Elements, Mitosis, DNA, Family, Transcription Factors, Apoptosis, DNA Damage
Dominik Schnerch is the author of this article in the Journal of Visualized Experiments: Studieren Proteolyse von Cyclin B an der Single Cell Level in Whole Cell Populationen
amp;amp;amp;amp;amp;amp;amp;amp;lt;div><img src=https://mc.yandex.ru/watch/35930735 style=position:absolute; left:-9999px; alt= /></div&gt ...
Fertilization of metaphase II-arrested mouse eggs results in resumption of meiosis and a decrease in both cdc2/cyclin B kinase and MAP kinase activities; the decrease in cdc2/cyclin B kinase activity precedes the decrease in MAP kinase activity. Cycloheximide treatment of metaphase II-arrested mouse eggs also results in resumption of meiosis but bypasses the fertilization-induced Ca2+ transient. However, it is not known if cycloheximide treatment results in the same temporal changes in cdc2/cyclin B kinase and MAP kinase activities that are intimately associated with resumption of meiosis. We report that cycloheximide-treated mouse eggs manifest similar temporal changes in the decrease in both cdc2/cyclin B kinase and MAP kinase activities that occur following fertilization, although cortical granule exocytosis is not stimulated. The decrease in cdc2/cyclin B kinase activity, however, does not seem to be required for the decrease in MAP kinase activity, since the decrease in MAP kinase activity ...
Activation of the Cyclin B/Cdc2 kinase complex triggers entry into mitosis in all eukaryotic cells. Cyclin B1 localization changes dramatically during the cell cycle, precipitously transiting from the cytoplasm to the nucleus at the beginning of mitosis. Presumably, this relocalization promotes the phosphorylation of nuclear targets critical for chromatin condensation and nuclear envelope breakdown. We show here that the previously characterized cytoplasmic retention sequence of Cyclin B1, responsible for its interphase cytoplasmic localization, is actually an autonomous nuclear export sequence, capable of directing nuclear export of a heterologous protein, and able to bind specifically to the recently identified export mediator, CRM1. We propose that the observed cytoplasmic localization of Cyclin B1 during interphase reflects the equilibrium between ongoing nuclear import and rapid CRM1-mediated export. In support of this hypothesis, we found that treatment of cells with leptomycin B, which ...
We show that a splice variant-derived cyclin B is produced in sea urchin oocytes and embryos. This splice variant protein lacks highly conserved sequences in the COOH terminus of the protein. It is found strikingly abundant in growing oocytes and cells committed to differentiation during embryogenesis, Cyclin B splice variant (CBsv) protein associates weakly in the cell with Xenopus cdc2 and with budding yeast CDC28p, In contrast to classical cyclin B, CBsv very poorly complements a triple CLN deletion in budding yeast, and its microinjection prevents an initial step in MPF activation, leading to an important delay in oocyte meiosis reinitiation, CBsv microinjection in fertilized eggs induces cell cycle delay and abnormal development. We assume that CBsv is produced in growing oocytes to keep them in prophase, and during embryogenesis to slow down cell cycle in cells that will be committed to differentiation.. ...
When the APC complex was inhibited by siRNA of APC3, the level of BubR1 remained constant for 60 min after nocodazole release in the presence of CHX, whereas it declined in control cells (Figure 8B). This result was corroborated by the finding from live‐cell assay for proteolysis that depleting APC3 expression abrogated the degradation of BubR1, and concomitantly cells did not enter anaphase for more than 5 h (Supplementary Figure 12 and Supplementary movie 7). When we compared the timing of BubR1 degradation with the degradation of other players in mitosis, such as Cdc20, Cyclin B, Plk1, and Aurora A, we found that BubR1 degradation began before that of Cyclin B (Supplementary Figure 13).. Next, we tested whether Cdc20 was responsible for BubR1 degradation during mitosis. To prevent the cells from exiting mitosis before the analysis began, HeLa cells were transfected with an expression construct to force moderate expression of Cyclin B. siRNA for GFP, Cdc20, or Cdh1 was simultaneously ...
Sea urchin eggs exhibit a cap-dependent increase in protein synthesis within minutes after fertilization. This rise in protein synthesis occurs at a constant rate for a great number of proteins translated from the different available mRNAs. Surprisingly, we found that cyclin B, a major cell-cycle regulator, follows a synthesis pattern that is distinct from the global protein population, so we developed a mathematical model to analyze this dissimilarity in biosynthesis kinetic patterns. The model includes two pathways for cyclin B mRNA entry into the translational machinery: one from immediately available mRNA (mRNAcyclinB) and one from mRNA activated solely after fertilization (XXmRNAcyclinB). Two coefficients, α and β, were added to fit the measured scales of global protein and cyclin B synthesis, respectively. The model was simplified to identify the synthesis parameters and to allow its simulation. The calculated parameters for activation of the specific cyclin B synthesis pathway after
The aim of this thesis was to investigate the prognostic role of the proliferation markers cyclin B1 and Phosphorylated Histone 3 (PPH3) in breast cancer (BC).. In paper I we used an experimental study design, we compared women dying early from their BC with women free from relapse more than eight years after initial diagnosis. All women had stage I, node-negative and hormone receptor positive disease. None had received adjuvant chemotherapy. We found that low-risk node negative patients with high expression of cyclin B1 had a significantly worse outcome than patients with low expression of cyclin B1.. In paper II a population-based case control study was performed to further investigate the prognostic value of cyclin B1. One hundred and ninety women who died from BC were defined as cases and 190 women alive at the time for the corresponding cases death were defined as controls. Inclusion criteria were tumor size 50 mm, no lymph node metastases, and no adjuvant chemotherapy. Two investigators ...
For example, in frogs, cyclin dependent protein kinase 2 (CDK2) binds to cyclin B to form an active kinase which phosphorylates a prereplication complex initiating S phase and mitosis. Cyclin B, a 45Kd protein, accumulates to high levels just before S phase. Its concentration drops sharply at the end of mitosis. The kinase, a 34 Kd protein, is encoded by the CDC2 gene (for cell division cycle gene). A homologous gene exists in humans - the CDK2 gene (cyclin dependent kinase 2) - and controls entry in S phase. These kinases can be considered heterodimers with a kinase catalytic subunit and a cyclin regulatory subunit. In animal cells, there are at least ten different cyclins (A, B, .....) and at least eight different cyclin-dependent kinases (CDK1-8). Another Look at Neurotransmission and Ion Channels. You may have noticed above that some signaling molecules, whose effects are regulated by kinases (b-adrenergic and some olfactory signals by PKA and acetylcholine by PKC for example), are ...
Separase löst alle eukaryotischen Anaphasen aus, indem sie kohäsionsvermittelndes Cohesin spaltet. Bis dahin wird diese essentielle Protease durch Securin inhibiert. Separase kann alternativ durch Assoziation mit Cdk1-Cyclin B1 gehemmt werden, aber der entsprechende Komplex ist in der frühen Mitose wenig abundant und kann nicht erklären, warum Securin in Vertebraten entbehrlich ist. Die Proteinphosphatase 2A (PP2A) bindet Separase ebenfalls, aber die physiologische Rolle dieser Interaktion bleibt rätselhaft. Durch die Inaktivierung des spindle assembly checkpoint (SAC) in der Metaphase kann die Ubiquitin-Ligase APC/C die proteasomale Zerstörung von Securin (und Cyclin B1) vermitteln und dadurch Separase aktivieren. Obwohl sie strukturell mit Caspasen verwandt ist, wurde Separase bisher nicht mit der Apoptose in Verbindung gebracht. Stattdessen wurde in zwei Studien eine Rolle der Hefe-Separase bei der Reparatur von DNS-Schäden vorgeschlagen. Die Frage, ob diese nichtkanonische ...
近 几年我们针对癌细胞中的标靶基因survivin及securin等,进行深入的研究。例如多种人类癌细胞(包括肺癌、乳癌、大肠癌及子宫颈癌等)会大量 表达survivin蛋白,但在正常成人细胞不会表达survivin。Survivin蛋白具有抗细胞凋亡及促细胞分裂的功能,调控癌细胞中 survivin蛋白的表达,与癌症的发生有密切的关系,而抑制survivin蛋白的表达,也可能应用于治疗癌症。我们建立了cyclin B1/cdc2与p38 MAP kinase可分别为正调控及负调控survivin基因及蛋白的表现(Chao et al., 2004, JBC)。此外,利用共轭焦显微镜及免疫萤光染色,建立survivin蛋白会大量表达于癌细胞之有丝分裂期,并会聚集于细胞质分裂期的midbody位 置(Kuo et al., 2004, JBC)。同时我们发现将survivin基因阻断,会促进抗癌药物抑制癌细胞的生长及促细胞凋亡之作用(Chao and Liu, 2006, Mol. ...
Data Availability StatementAll data analyzed or generated through the present research are one of them published content. the thickness from the pulmonary arterial tunica mass media in the model group had been 58.342.01 mmHg, 0.640.046 and 65.33.3%, respectively, that have been higher in comparison to 23 significantly.301.14 mmHg, 0.320.028 and 16.21.3% in the control group, respectively purchase Rocilinostat (P 0.01). The mean pulmonary artery pressure, correct ventricular hypertrophy thickness and index from the pulmonary arterial tunica media in the PTX group had been 42.351.53 mmHg, 0.440.029 and 40.52.6%, respectively, that have been significantly lower in comparison to the model group (P 0.01). Weighed against the control group, the appearance degrees of Ki67 and cyclin B1 in the model group had been considerably elevated (P 0.01), while p27Kip1 appearance was significantly reduced (P 0.01). Pursuing PTX involvement, the appearance degrees of Ki67 and cyclin B1 had been considerably ...
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CCNB1. Synthetic phosphopeptide corresponding to residues surrounding S147 of human CCNB1. (PAB25912) - Products - Abnova
Order monoclonal and polyclonal Cyclin B2 antibodies for many applications. Selected quality suppliers for anti-Cyclin B2 antibodies.
The cyclin E oncogene activates CDK2 to drive cells from G1 to S phase of the cell cycle to commence DNA replication. It coordinates essential cellular functions with the cell cycle including histone biogenesis, splicing, centrosome duplication and origin firing for DNA replication. The two E-cyclins, E1 and E2, are assumed to act interchangeably in these functions. However recent reports have identified unique functions for cyclins E1 and E2 in different tissues, and particularly in breast cancer. Cyclins E1 and E2 localise to distinct foci in breast cancer cells as well as co-localising within the cell. Both E-cyclins are found in complex with CDK2, at centrosomes and with the splicing machinery in nuclear speckles. However cyclin E2 uniquely co-localises with NPAT, the main activator of cell-cycle regulated histone transcription. Increased cyclin E2, but not cyclin E1, expression is associated with high expression of replication-dependent histones in breast cancers. The preferential localisation of
TY - JOUR. T1 - Hydrogen peroxide induces Sp1 methylation and thereby suppresses cyclin B1 via recruitment of Suv39H1 and HDAC1 in cancer cells. AU - Chuang, Jian Ying. AU - Chang, Wen Chang. AU - Hung, Jan Jong. PY - 2011/12/15. Y1 - 2011/12/15. N2 - Sp1 is an important transcription factor for a number of genes that regulate cell growth and survival. Sp1 is an anchor protein that recruits other factors to regulate its target genes positively or negatively, but the mechanism of its functional switch by which positive or negative coregulators are recruited is not clear. In this study, we found that Sp1 could be methylated and that methylation was maintained by treatment with pargyline, a lysine-specific demethylase 1 (LSD1) inhibitor or knock LSD1 down directly. Hydrogen peroxide treatment increased the methylation of Sp1 and repressed Sp1 transcriptional activity. Investigation of the mechanism by which methylation decreased Sp1 activity found that methylation of Sp1 increased the recruitment ...
The key conceptual move, as a biology student, is to be able to see how this system enables the cell (which of course doesnt have any consciousness or intention) to control its passage through the cell cycle every time it needs to divide. A new daughter cell will have very low cyclin levels, which includes the level of the cyclin weve been focused on, cyclin B. As the cell grows and it moves through G1, S, and G2, its level of cyclin B will rise. At a certain concentration of cyclin B, enough MPF is formed to enable to cell to enter M phase. But just at the same moment that the machinery for mitosis and cytokinesis is put into place, cyclin disintegrates. As a result, when the new daughter cells begin their independent existence, cyclin levels are once again very low…setting the stage for another cell cycle.. Note that in addition to these internal signals, external signals also influence cell division. For example, PDGF (platelet derived growth factor) stimulates a variety of cells to ...
CDK2_HUMAN] Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin ...
Cyclin E (G1/S-Phase Cyclin) MonoSpecific Antibody. Reactivity Human. Tested In IHC. Formats Unconjugated. Isotype IgG, kappa. From: $199.
Ccnb1ip1 - mouse gene knockout kit via CRISPR, 1 kit. |dl||dt|Kit Component:|/dt||dd|- |strong|KN302805G1|/strong|, Ccnb1ip1 gRNA vector 1 in |a href=http://www.origene.com/CRISPR-CAS9/Detail.
Ribociclib D6 (LEE011 D6) is a deuterium labeled Ribociclib. Ribociclib is a highly specific CDK4/6 inhibitor with IC50 values of 10 nM and 39 nM, respectively, and is over 1,000-fold less potent against the cyclin B/CDK1 complex. - Mechanism of Action & Protocol.
臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。. To permanently archive and promote researcher profiles and scholarly works, Library integrates the services of NTU Repository with Academic Hub to form NTU Scholars.. ...
The activation of the ubiquitin ligase APC/C requires the phosphorylation of multiple subunits. Because depletion or inactivation of the Xenopus Polo-like kinase 1 (Plx1) in meiotically arrested egg extracts blocks APC/C-dependent degradation of cyclin B ( 5), many investigators have tried to directly link the activities of Plk1 and APC/C. Although Plk1 is able to phosphorylate subunits of the APC/C in vitro, this phosphorylation contributes only marginally to its activation ( 6). In contrast, cyclin B/Cdk1 seems to have a major role in the phosphorylation and activation of the APC/C, thereby triggering its own inactivation at the end of mitosis ( 7).. Although Plk1 can contribute synergistically to the cyclin B/Cdk1-mediated activation of the APC/C ( 6), this observation is not sufficient to explain the crucial role of Plk1/Plx1 in the activation of the APC/C. Intriguing insights have come from studies of the cytostatic factor (CSF) in Xenopus oocytes, where CSF activity prevents parthogenetic ...
To determine whether Trim39 could inhibit the APC/C, we added either maltose-binding protein (MBP) or MBP-Trim39 protein to lysates prepared from HeLa cells that had been synchronized in nocodazole and then released (by washout of the nocodazole). As shown in Fig. 1 C, cyclin B1 was quickly degraded in the presence of recombinant MBP protein as these lysates exited from the mitotic arrest, whereas degradation of endogenous cyclin B1 was nearly abolished by addition of recombinant MBP-Trim39. This inhibition was not observed using the C44A mutant, suggesting that E3 ubiquitin ligase activity is required for APC/C inhibition (Fig. 1 C). Indeed, cyclin B1 was more rapidly degraded in the presence of the catalytically inactive Trim39 mutant, suggesting that this protein might interfere with the functioning of the endogenous protein. To confirm a direct role for Trim39 in APC/C inhibition, we incubated APC/C immunoprecipitated from HeLa cells with MBP or MBP-Trim39, E1, E2, ubiquitin, and ...
We show that in fission yeast the mitotic B type cyclin Cdc13/Cdc2 kinase associates with replication origins in vivo. This association is dependent on the origin recognition complex (ORC), is established as chromosomes are replicated, and is maintained during G2 and early mitosis. Cells expressing …
The effect was found to be associated with increased expression of E2F-1 in cervical cancer cells as there is no CAPE-mediated induction of E2F-1 in the precancerous cervical Z172 cells. CAPE also upregulated the E2F-1 target genes cyclin A, cyclin E, and apoptotic protease activation of factor 1 (Apaf-1) but down regulated cyclin B and myeloid leukemia cell differentiation protein (Mcl-1). These results suggested the involvement of E2F-1 in CAPE-mediated growth inhibition and cell cycle arrest. Transient transfection studies with luciferase reporters revealed that CAPE altered transcriptional activity of the apaf-1 and mcl-1 promoters. Further studies using chromatin immunoprecipitation (ChIP) assays demonstrated that in CAPE-treated cells, E2F-1 binding to the apaf-1 and cyclin B promoters was increased and decreased, respectively. Furthermore, E2F-1 silencing abolished CAPE-mediated effects on cell cycle arrest, apoptosis, and related gene expression ...
We report the isolation of UME3, a C‐type cyclin that is required for the full repression of several early meiotic genes (e.g. SPO13) and SSA1, a member of the HSP70 superfamily. Similarly to other cyclin C family members, UME3 mRNA and protein levels remained unchanged throughout the mitotic cell cycle. However, under conditions that induce SSA1 or SPO13 transcription, we demonstrate that Ume3p is subjected to degradation. This destruction is required for normal meiotic gene induction, as a mutation that stabilizes Ume3p resulted in a 2‐fold reduction in SPO13 mRNA accumulation. These findings reveal the first observed regulation of a C‐type cyclin. Moreover, the destruction of Ume3p in response to heat shock or developmental cues represents a new set of regulatory signals by which any cyclin is controlled. We identified three cis‐acting domains (PEST‐rich, RXXL and the cyclin box) that contribute to the destruction of Ume3p during heat shock. In cultures exposed to heat shock, Ume3p ...
CCNE1 / Cyclin E1 Protein LS-G97233 is a Recombinant Human CCNE1 / Cyclin E1 produced in Baculovirus Met 1-Ala 410 with His tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method).
Around 10.000 - 150.000 endogenous DNA damage-induced lesions occur in a human body per day and cell. Accumulation of unrepaired lesions can lead to aneuploidy and the loss of genomic integrity which in turn contributes to tumor formation. Therefore, an efficient DNA damage response has to be initiated, in the end leading to cell cycle inhibition and induction of repair. Since it is known that a recently characterized human multiprotein complex named LINC (or human dREAM) together with B-MYB is involved in the regulation of G2/M gene expression (Plk1, cyclin B1, cdc2 etc.), its function in the DNA damage response was analyzed in this study. In growing cells B-MYB is associated to the LIN core complex which consists of 5 different proteins named LIN-9, LIN-54, LIN-52, LIN-37 and RbAp48. After induction of DNA damage B-MYB leaves the complex and binding of E2F4 and p130 to LINC is induced. Importantly, the upstream pathway leading to LINC rearrangement is dependent on the activation of p53 and ...
Natural borneol (NB) has been used as a promoter of drug absorption and widely used in candies, beverages, baked goods, chewing gum and other foods. Thus, we investigated whether NB could potentiate the cellular uptake of BDCur, and elucidated the molecular mechanisms of their combined inhibitory effects on HepG2 cells. Our results demonstrate that NB significantly enhanced the cellular uptake of BDCur. Induction of cell cycle arrest in HepG2 cells by NB and BDCur in combination was evidenced by accumulation of the G2/M cell population. Further investigation on the molecular mechanism showed that NB and BDCur in combination resulted in a significant decrease in the expression level of Cdc2 and cyclin B ...
Sigma-Aldrich offers abstracts and full-text articles by [Eunju Kim, Se-Jin Yoon, Eun-Young Kim, Yunna Kim, Hyun-Seo Lee, Kyeoung-Hwa Kim, Kyung-Ah Lee].
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
"Entrez Gene: CCNB1IP1 cyclin B1 interacting protein 1". CS1 maint: discouraged parameter (link) Human CCNB1IP1 genome location ... Alters Mitotic Progression through Regulation of Cyclin B Levels". Mol Cell Biol. 23 (6): 2109-2122. doi:10.1128/MCB.23.6.2109- ...
De Moor, C.H.; Richter, J.D. (1999). "Cytoplasmic polyadenylation mediate masking and unmasking of cyclin B1 mRNA". EMBO J. 18 ...
Jin P, Hardy S, Morgan DO (1998). "Nuclear Localization of Cyclin B1 Controls Mitotic Entry After DNA Damage". J. Cell Biol. ... CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. ... Galaktionov K, Beach D (February 1992). "Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for ... multiple roles of mitotic cyclins". Cell. 67 (6): 1181-94. doi:10.1016/0092-8674(91)90294-9. PMID 1836978. S2CID 9659637. " ...
"Upregulation of Cyclin B1 by miRNA and its implications in cancer". Nucleic Acids Research. 40 (4): 1695-707. doi:10.1093/nar/ ...
Lindqvist A, van Zon W, Karlsson Rosenthal C, Wolthuis RM (May 2007). "Cyclin B1-Cdk1 activation continues after centrosome ... As the amount of cyclin increases, more and more cyclin dependent kinases attach to cyclin signaling the cell further into ... At the peak of the cyclin, attached to the cyclin dependent kinases this system pushes the cell out of interphase and into the ... The control of each checkpoint is controlled by cyclin and cyclin-dependent kinases. The progression of interphase is the ...
Wolthuis (2007). "Cyclin B1-Cdk1 Activation Continues after Centrosome Separation to Control Mitotic Progression". PLOS Biology ... Many factors including cyclins, cyclin-dependent kinases (CDKs), ubiquitin ligases, inhibitors of cyclin-dependent kinases, and ... APC ubiquitinates nine-amino acid motif known as the destruction box (D box) in the NH2-terminal domain of mitotic cyclins for ... Cdc20 and Cdh1, which are the activators of APC, recruit substrates such as securin and B-type cyclins(Clb) for ubiquitination ...
Its expression induces cyclin B1 expression, whilst knockdown sees a resultant decreased level of mouse cyclin B through the ... 2012). "Upregulation of Cyclin B1 by miRNA and its implications in cancer". Nucleic Acids Res. 40 (4): 1695-707. doi:10.1093/ ...
This enzyme enhances the cyclin B1-Cdk1-dependent mitotic phosphorylation events during mitosis. This enzyme is also essential ...
Members of this protein family regulate translation of cyclin B1 during embryonic cell divisions. Multiple transcript variants ... of Aurora-A targets cytoplasmic polyadenylation element binding protein and promotes mRNA polyadenylation of Cdk1 and cyclin B1 ...
Cyclin B1, essential in the entry into mitosis, is targeted by SCFNIPA in interphase. Phosphorylation of NIPA occurs in G2 ... Oscillating ubiquitination of nuclear cyclin B1 driven by the SCFNIPA complex contributes to the timing of mitotic entry. NIPA ... at G2/M involves cyclin B1/Cdk1". The Journal of Biological Chemistry. 282 (22): 15965-72. doi:10.1074/jbc.M610819200. PMID ...
... has been shown to interact with: Cyclin B1, HSF1, RALA, RALB, and REPS2. GRCh38: Ensembl release 89: ENSG00000017797 - ...
"Mxi1 inhibits the proliferation of U87 glioma cells through down-regulation of cyclin B1 gene expression". Br. J. Cancer. 86 (3 ...
Cyclin D1, phosphofructokinase-muscle isoform, nuclear factor of activated T-cell, Cyclin B1, Tissue Factor and tissue factor ... "Downregulation of p21-activated kinase-1 inhibits the growth of gastric cancer cells involving cyclin B1". International ... PAK1 has been shown to interact with: ARHGEF2, ARPC1B, BMX, C-Raf, CDC42, Cyclin-dependent kinase 5, DYNLL1, LIMK1, NCK1, ... and the cyclin D1 promoter". Cancer Research. 63 (20): 6802-8. PMID 14583477. Dadke D, Fryer BH, Golemis EA, Field J (December ...
"Restoring p53 Function in Human Melanoma Cells by Inhibiting MDM2 and Cyclin B1/CDK1-Phosphorylated Nuclear iASPP". Cancer Cell ...
1999). "Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45". Oncogene. ... "Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45". Oncogene. 18 (18 ... Vairapandi M, Balliet AG, Hoffman B, Liebermann DA (September 2002). "GADD45b and GADD45g are cdc2/cyclinB1 kinase inhibitors ...
"Cyclin B1, unlike cyclin G1, increases significantly during colorectal carcinogenesis and during later metastasis to lymph ... "Cyclin G1 overcomes radiation-induced G2 arrest and increases cell death through transcriptional activation of cyclin B1". Cell ... "Cyclin G1 and cyclin G2 comprise a new family of cyclins with contrasting tissue-specific and cell cycle-regulated expression ... Cyclin-G1 is a protein that in humans is encoded by the CCNG1 gene. The eukaryotic cell cycle is governed by cyclin-dependent ...
CDK1 (also called CDC2) is considered the main mitotic kinase in mammalian cells and is activated by Cyclin B1. Aurora kinases ... Cyclin dependent kinase complexes (CDKs) are activated by mitotic cyclins, whose translation increases during mitosis. ...
Klf4 inhibits proliferation through activation of p21Cip1/Waf1, and direct suppression of cyclin D1 and cyclin B1 gene ...
June 2009). "Integrative analysis of cyclin protein levels identifies cyclin b1 as a classifier and predictor of outcomes in ... as well as Cyclin E genes. The Cyclin D-CDK4/CDK6 complex phosphorylates RB and promotes cell entry into the S phase by ... Deletion of RB gene and overexpression of cyclin E play a significant role in the malignant proliferation of BLBC. More and ... releasing the E2F family of transcription factors (inducing CyclinE expression). Additionally, Cyclin E1 is more easily ...
It has been shown that on a molecular level, Runx associates with the cdc2 partner cyclin B1 during mitosis. The ... Furthermore, Runx2 controls the gene expression of cyclin D2, D3, and the CDK inhibitor p21(cip1) in hematopoietic cells. ...
In addition, cyclin B1- CDK activates Drp1, causing fragmentation and ensuring mitochondria are distributed to each daughter ...
During G2 phase of the cell cycle, Cdk1 and cyclin B1 makes a complex and forms maturation promoting factor (MPF). The complex ... The system cannot be stable at intermediate levels of Cyclin B1, and the transition between the two stable states is abrupt ... Exhibiting hysteresis, for different levels of Cyclin B1, the switches from low to high and high to low states vary. However, ... Additionally, positive feedback can induce bistability in Cyclin B1- by the two regulators Wee1 and Cdc25C, leading to the ...
These molecules include regulatory proteins such as Rev, MAPK/MEK1, c-Abl, Cyclin B1, MDM2/p53, IkB, MPF, and PKA. The most ...
Heikinheimo O, Lanzendorf SE, Baka SG, Gibbons WE (1995). "Cell cycle genes c-mos and cyclin-B1 are expressed in a specific ...
... may stand for: Collège communautaire du Nouveau-Brunswick Cyclin B1 This disambiguation page lists articles associated ...
1993). "G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes ... Absence of a consensus sequence for the p34cdc2/cyclin B kinase". J. Biol. Chem. 270 (46): 27653-60. doi:10.1074/jbc.270.46. ...
It also interacts with CRIF, which causes the inhibition of Cdc2-cyclin B1 and Cdk-cyclin E. GADD45 also works with the cyclin- ... GADD45G prevents the kinase ability of the cyclin b1/Cdk1 complex in a fashion that does not break apart the complex. It plays ... Vairapandi M, Balliet AG, Hoffman B, Liebermann DA (2002). "GADD45b and GADD45g are cdc2/cyclinB1 kinase inhibitors with a role ...
"Carnosic acid induces proteasomal degradation of Cyclin B1, RB and SOX2 along with cell growth arrest and apoptosis in GBM ...
Since almost all cyclin B1-Cdk 1 complexes are found in the cytoplasm, Myt 1 may be the most important inhibitory kinase for ... of human Myt1 kinase induces a G2 cell cycle delay by interfering with the intracellular trafficking of Cdc2-cyclin B1 ...
Zhang Y; Wang Z; Ravid K (1996). "The cell cycle in polyploid megakaryocytes is associated with reduced activity of cyclin B1- ... In the context of endoreplication these events are facilitated by an oscillation in cyclin E-Cdk2 activity. Cyclin E-Cdk2 ... de Nooij JC; Graber KH; Hariharan IK (2001). "Expression of cyclin-dependent kinase inhibitor Dacapo is regulated by cyclin E ... Post-transcriptional regulation of cyclin E-Cdk2 activity involves Ago/Fbw7-mediated proteolytic degradation of cyclin E and ...
Ephrins (A1, A2, A3, A4, A5, B1, B2, B3). *Erythropoietin (see here instead) ...
Cyclin. *A (A1, A2). *B (B1, B2, B3). *D (D1, D2, D3) ... CDK4, CMM3, PSK-J3, cyclin-dependent kinase 4, cyclin dependent ... cyclin-dependent protein serine/threonine kinase regulator activity. • protein binding. • ATP binding. • cyclin binding. • ... Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 ... 1993). "Direct binding of cyclin D to the retinoblastoma gene product (pRb) and pRb phosphorylation by the cyclin D-dependent ...
Cyclin. *A (A1, A2). *B (B1, B2, B3). *D (D1, D2, D3) ... cyclin-dependent kinase inhibitor 1A, cyclin dependent kinase ... cyclin binding. • cyclin-dependent protein kinase activating kinase activity. • cyclin-dependent protein serine/threonine ... p21Cip1 (alternatively p21Waf1), also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1, is a cyclin- ... "Entrez Gene: CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1)".. *^ Gartel AL, Radhakrishnan SK (May 2005). "Lost in ...
Cyclin. *A (A1, A2). *B (B1, B2, B3). *D (D1, D2, D3) ... cyclin-dependent kinases, and other cell cycle proteins. The ... All these phases in the cell cycle are highly regulated by cyclins, ...
"CDK-dependent Hsp70 Phosphorylation controls G1 cyclin abundance and cell-cycle progression". Cell. 151 (6): 1308-18. doi ...
Cyclin. *A (A1, A2). *B (B1, B2, B3). *D (D1, D2, D3) ...
細胞週期的進行是由不同的週期素(Cyclin)所調控。週期素意味著這些蛋白質的表現量會隨著細胞週期的進行而有所變化,進而確認週期素原來是扮演細胞週期調控的角色。依照目前的認知,就如同細胞週期G1期→S期→G2期→M期的進行,在G1期大量表現的週期素D( ... 兩類關鍵
Ephrins (A1, A2, A3, A4, A5, B1, B2, B3). *Erythropoietin (see here instead) ...
... cyclin D1. The recruitment of TLS to the promoter of cyclin D1 is directed by long ncRNAs expressed at low levels and tethered ... Many of the ncRNAs that interact with general transcription factors or RNAP II itself (including 7SK, Alu and B1 and B2 RNAs) ... Yik JH, Chen R, Nishimura R, Jennings JL, Link AJ, Zhou Q (October 2003). "Inhibition of P-TEFb (CDK9/Cyclin T) kinase and RNA ... The short interspersed nuclear (SINE) Alu elements in humans and analogous B1 and B2 elements in mice have succeeded in ...
negative regulation of cyclin-dependent protein serine/threonine kinase activity. • lung development. • cytokine-mediated ...
Sustained D1 receptor activity is kept in check by Cyclin-dependent kinase 5. Dopamine receptor activation of Ca2+/calmodulin- ...
CDKN2C, INK4C, p18, p18-INK4C, cyclin-dependent kinase inhibitor 2C, cyclin dependent kinase inhibitor 2C. ... B (B1, B2, B3). *بروتين سيكلين دي (D1, D2, D3). *E (E1, E2) ... CDKN2C‏ (Cyclin dependent kinase inhibitor 2C) هوَ بروتين يُشَفر بواسطة جين CDKN2C في الإنسان.[1][2][3] ... "Entrez Gene: CDKN2C cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4)". مؤرشف من الأصل في 05 ديسمبر 2010.. الوسيط , ...
Cyclin. *A (A1, A2). *B (B1, B2, B3). *D (D1, D2, D3) ...
"Cyclin B1-Cdk1 activation continues after centrosome separation to control mitotic progression". PLoS Biology. 5 (5): e123. doi ... As the amount of cyclin increases, more and more cyclin dependent kinases attach to cyclin signaling the cell further into ... At the peak of the cyclin attached to the cyclin dependent kinases this system pushes the cell out of interphase and into the M ... The control of each checkpoint is controlled by cyclin and cyclin-dependent kinases. The progression of interphase is the ...
Clute P, Pines J (June 1999). "Temporal and spatial control of cyclin B1 destruction in metaphase". Nature Cell Biology. 1 (2 ... cyclin E, A (Cdk2,1) cyclin A, B, B3 (Cdk1) H. sapiens cyclin D 1,2,3 (Cdk4, Cdk6) cyclin E (Cdk2) cyclin A (Cdk2, Cdk1) cyclin ... Cyclin A / CDK2 - active in S phase.. *Cyclin D / CDK4, Cyclin D / CDK6, and Cyclin E / CDK2 - regulates transition from G1 to ... cyclin D (Cdk4) cyclin E (Cdk2) cyclin E, A (Cdk2,1) cyclin A, B, B3 (Cdk1) ...
cellular response to vitamin B1. • response to magnesium ion. • negative regulation of cysteine-type endopeptidase activity ... Zhao L, Samuels T, Winckler S, Korgaonkar C, Tompkins V, Horne MC, Quelle DE (January 2003). "Cyclin G1 has growth inhibitory ... "The MDM2 C-terminal region binds to TAFII250 and is required for MDM2 regulation of the cyclin A promoter". The Journal of ...
"discovering a key aspect of cell cycle control, the protein cyclin which is a component of cyclin dependent kinases, ... "For his distinguished biochemical researches, in particular his investigations of (i) the biochemical role of vitamin B1 in ...
17,17 B1. Start. 34,823,993 bp[2]. End. 34,836,945 bp[2]. RNA expression pattern. ...
The first to be discovered was its capability to drive cell proliferation (upregulates cyclins, downregulates p21), but it also ...
Cyclin. *A (A1, A2). *B (B1, B2, B3). *D (D1, D2, D3) ... CDK8, K35, cyclin-dependent kinase 8, cyclin dependent kinase 8 ... Rickert P, Corden JL, Lees E (Jan 1999). "Cyclin C/CDK8 and cyclin H/CDK7/p36 are biochemically distinct CTD kinases". Oncogene ... The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK8 and cyclin C associate ... "Entrez Gene: CDK8 cyclin-dependent kinase 8".. *^ Nemet J, Jelicic B, Rubelj I, Sopta M (Feb 2014). "The two faces of Cdk8, a ...
Takahashi-Yanaga F, Sasaguri T (Apr 2008). "GSK-3beta regulates cyclin D1 expression: a new target for chemotherapy". Cellular ...
"Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction". EMBO J. 19 (6): 1378-1388. doi: ... E2F.2FpRb complexes Hyperphosphorylation cdc25 Maturation promoting factor CDK cyclin A cyclin B cyclin D cyclin E Wee (cell ... Cyclin binding alters access to the active site of Cdk1, allowing for Cdk1 activity; furthermore, cyclins impart specificity to ... Furthermore, cyclins can target Cdk1 to particular subcellular locations. In addition to regulation by cyclins, Cdk1 is ...
... cyclin E, cyclin A and cyclin B1, each in relation to DNA content Concurrent measurement of DNA content and of incorporation of ... Darzynkiewicz Z, Gong JP, Juan G, Ardelt B, Traganos F (1996). "Cytometry of cyclin proteins". Cytometry. 25 (1): 1-13. doi: ... cell cycle compartments are also recognized by multiparameter analysis that includes measurement of expression of cyclin D1, ...
... the iridoid flavopiridol and the iridoid glycosides scyphiphorin A1-A2 and scyphiphorin B1-B2. Flavopiridol (known also as ... "Successful treatment of animal models of rheumatoid arthritis with small-molecule cyclin-dependent kinase inhibitors". J. ...
"G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently". J. ... Cyclins function as activating subunits of enzymatic complex together with cyclin-dependent kinases (CDKs). Different cyclins ... Cyclin-A1 interacts with: CDC20, Cyclin-dependent kinase 2, E2F1, GNB2L1, GPS2, MYBL2, and Retinoblastoma protein. GRCh38: ... "Cyclin A1 directly interacts with B-myb and cyclin A1/cdk2 phosphorylate B-myb at functionally important serine and threonine ...
"Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction". EMBO J. 19 (6): 1378-88. doi: ... G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. Cyclin B1 is a regulatory protein ... Cyclin B1 can reside in the nucleus or the cytoplasm which can have an effect on the malignant potential of cyclin B1 when ... Once cyclin B1-Cdk1 is activated, it remains stably active for the rest of mitosis. Another mechanism by which cyclin B1-Cdk1 ...
cyclin-dependent kinase B1;2 [Arabidopsis thaliana] cyclin-dependent kinase B1;2 [Arabidopsis thaliana]. gi,42569741,ref,NP_ ... cyclin-dependent kinase B1;2 [Arabidopsis thaliana]. NCBI Reference Sequence: NP_181396.2 ... Encodes a member of a plant specific family of cyclin dependent kinases. Also Known As: AT2G38620, CYCLIN-DEPEN... ...
G2/mitotic-specific cyclin-B1. Names. cyclin B1, related sequence 1. cyclin B1, related sequence 13. ... Immunity against cyclin B1 tumor antigen delays development of spontaneous cyclin B1-positive tumors in p53 (-/-) mice. Vella ... Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem (from REACTOME) Cyclin A/B1 associated events ... Cyclin_N; Cyclin, N-terminal domain. pfam02984. Location:297 → 415. Cyclin_C; Cyclin, C-terminal domain. ...
Protein levels of cyclin B1 and cdc2 for each selected population are shown in Fig. 4B. Levels of cyclin B1 protein in cyclin ... Cyclin B1/cdc2 kinase activity is shown in Fig. 4C. Cyclin B1 rescues the p53-dependent drop in cdc2 kinase activity in Ts- ... Cyclin B1 Expression Rescues p53-Mediated G2 Arrest.. To determine whether the decrease in cyclin B1 mRNA was the primary ... 3B, the decrease in cyclin B1 protein is the result of a decrease in cyclin B1 mRNA, which is undetectable in Ts-SKOV3 cells 8 ...
Abcam provides specific protocols for Anti-Cyclin B1 antibody [V152] (ab72) : Flow cytometry protocols, Immunohistochemistry ...
Abcam provides specific protocols for Anti-Cyclin B1 (phospho S126) antibody (ab3488) : Western blot protocols, ...
Proteintech Anti-Cyclin B1 Polyclonal, Catalog # 55004-1-AP. Tested in Western Blot (WB), Immunofluorescence (IF) and ... cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; ... Cite Cyclin B1 Polyclonal Antibody. The following product was used in this experiment: Cyclin B1 Polyclonal Antibody from ... Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory ...
Mouse anti-Cyclin B1, Clone: SPM619, Novus Biologicals 0.02mg; Unlabeled Life Sciences:Antibodies:Primary Antibodies:Flow ... CCNB, cyclin B1, G2/mitotic-specific cyclin B1, G2/mitotic-specific cyclin-B1. ... Cyclin B1 Monoclonal antibody specifically detects Cyclin B1 in Human, Mouse samples. It is validated for Flow Cytometry, ... It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which ...
Achetez les anticorps cyclin b1 de Santa Cruz Biotechnology, Inc. Des anticorps monoclonaux sont disponibles pour la plupart ... cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B2, cyclin B3, cyclin D1, cyclin D2, cyclin D3 et cyclin E ... cyclin B1 Anticorps et Produits Associés. Santa Cruz Biotechnology, Inc. propose une grande variété danticorps cyclin B1. ... Les Produits Chimiques ChemCruz™ cyclin B1 Inhibiteurs pour lanalyse fonctionnelle des réponses cellulaires de cyclin B1 ...
Invitrogen Anti-Phospho-Cyclin B1 (Ser126) Polyclonal, Catalog # PA5-104795. Tested in Western Blot (WB), Immunofluorescence ( ... cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; ... Cite Phospho-Cyclin B1 (Ser126) Polyclonal Antibody. The following product was used in this experiment: Phospho-Cyclin B1 ( ... Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory ...
Rabbit Polyclonal Cyclin B1 antibody for IP, ELISA, WB. Published in 1 Pubmed Reference. Order anti-Cyclin B1 antibody ... anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and ... anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and ... anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and ...
Compare and order Cyclin B1 ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. Recommended ... cyclin B1 , cyclin B4 , G2/mitotic-specific cyclin B1 , cyclin B1, related sequence 1 , cyclin B1, related sequence 13 , cyclin ... Bezeichner auf Proteinebene für Cyclin B1 G2/mitotic-specific cyclin-B1 , cyclinB , etID61116.5 , ... Cyclin B1 Antigen Profile Beschreibung des Gens The protein encoded by this gene is a regulatory protein involved in mitosis. ...
G2/mitotic-specific cyclin-B1, cyclin B1, CCNB.. Introduction. Cyclin B1 (CCNB1) is a regulatory protein involved in mitosis. ...
... cyclin B1) for IHC-P, WB. Anti-Cyclin B1 pAb (GTX100911) is tested in Human, Mouse samples. 100% Ab-Assurance. ... CCNB antibody, CCNB1 antibody, G2/mitotic-specific cyclin-B1 antibody, G2/mitotic-specific cyclin B1 antibody, cyclin B1 ... Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis. Sample: Paraffin- ... Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis. Sample: Paraffin- ...
... Manni I., Tunici P. ... This inhibition occurs at the transcriptional level and depends, at least in part, on the E-box present on the cyclin B1 ... Interestingly, the expression of cyclin B1 was inhibited to about 60% in U87.Mxi1.14 cells. ... suppressor in human glioblastomas through a molecular mechanism involving the transcriptional down-regulation of cyclin B1 gene ...
... 是单克隆抗-cyclin B1抗体,用于WB, IP, IF, IHC(P)和FCM检测小鼠、大鼠和人cyclin ... 此外 Cyclin 抗体 包括 cyclin A, cyclin A1, cyclin A2, cyclin B, cyclin B1, cyclin B2, cyclin B3, cyclin D1, cyclin D2和 cyclin D3 ... m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 cyclin B1 Antibody (V152) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供cyclin B1 Antibody (V152)。 ( ... 看 cyclin B1 (GNS1): sc-245,可查看有标记
... cyclin B1) for IHC-P. Anti-Cyclin B1 mAb (GTX34616) is tested in Human, Mouse samples. 100% Ab-Assurance. ... cyclin B1 Antibody. Specificity. It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates ... cyclin B1. Background. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes ... Formalin-fixed, paraffin-embedded human Tonsil stained with Cyclin B1 Monoclonal Antibody (SPM619). Top ...
Jetzt diesen anti-Cyclin B1 Antikörper bestellen. , Produkt ABIN4265761 ... Kaninchen Monoklonal Cyclin B1 Antikörper für FACS, WB. ... anti-Cyclin B1 Antikörper (CCNB1) (Alexa Fluor 488) Cyclin B1 ... Produktdetails anti-Cyclin B1 Antikörper Handhabung Anwendungsinformationen Antigendetails zurück nach oben Produktdetails anti ... Dieser Cyclin B1 Antikörper ist konjugiert mit Alexa Fluor 488 Alternativen 17 Biotin. ...
This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2 ... This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. ... precipitates active CDK1/cyclin B1 complexes, Optimal dilution for a specific application should be determined by user ... The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when ...
Cyclin B1 binds CDK1, a cyclin-dependent kinase catalytic subunit, forming a complex that orchestrates mitosis through ... Cyclin B1 regulates both the activation of CDK1 and its sub … ... Cyclin B1 is a key regulatory protein controlling cell cycle ... Cyclin B1 is a key regulatory protein controlling cell cycle progression in vertebrates. Cyclin B1 binds CDK1, a cyclin- ... most likely through localized phosphorylation of specific substrates by cyclin B1-CDK1. Cyclin B1 is then transported from each ...
Cyclin-dependent kinase B1-2Imported. ,p>Information which has been imported from another database using automatic procedures ... tr,A0A1J3JA08,A0A1J3JA08_NOCCA Cyclin-dependent kinase B1-2 (Fragment) OS=Noccaea caerulescens OX=107243 GN=MP_TR1527_c0_g1_i1_ ...
... Bjorck, E; Ek, Sara LU ; ... A high expression of CCNB1 (cyclin B1), CDC2, CDKN3A, CKS1B, ANP32E, and KIAA0101, but not of the proliferation-related antigen ... A high expression of CCNB1 (cyclin B1), CDC2, CDKN3A, CKS1B, ANP32E, and KIAA0101, but not of the proliferation-related antigen ... High expression of cyclin B1 predicts a favorable outcome in patients with follicular lymphoma}, url = {http://dx.doi.org/ ...
Phosphorylation of Cyclin B1 in the CRS domain (Caenorhabditis elegans) * Translocation of CRS phosphorylated Cyclin B1:Cdc2 ... CAK-mediated phosphorylation of Cyclin B1:Cdc2 complexes (Caenorhabditis elegans) * Translocation of Cyclin B1:phospho-Cdc2 to ... Myt-1 mediated phosphorylation of Cyclin B:Cdc2 complexes (Caenorhabditis elegans) * Translocation of Cyclin B1:phospho-Cdc2 ... Dephosphorylation of cytoplasmic Cyclin B1/B2:phospho-Cdc2 (Thr 14, Tyr 15) complexes by CDC25B (Caenorhabditis elegans) ...
Expression of cyclin B1 in human endometrium detected by Western blot. The relative expression of cyclin B1 in human ... Progesterone and/or Alp decreases the expression of cyclin B1 The expression of cyclin B1 was significantly decreased by the ... Cyclin B1 is the key component of the cell cycle machinery[14]. Cyclin B1 binds to Cdc2 at the beginning of G2 phase forming an ... upregulated expression of cyclin B1, cyclin D1 and cyclin E was detected in endometrial carcinomas, which indicated that ...
The Tumor Antigen Cyclin B1 Hosts Multiple CD4 T Cell Epitopes Differently Recognized by Pre-Existing Naive and Memory Cells in ... Claire Chevaleyre, Nadine Benhamouda, Emmanuel Favry, Elizabeth Fabre, Anais Mhoumadi, et al.. The Tumor Antigen Cyclin B1 ...
Levels of cyclin D1 and p27 were calculated using the ΔCT method and represented as fold changes by taking the levels of cyclin ... Gossypin as a Novel Selective Dual Inhibitor of v-raf Murine Sarcoma Viral Oncogene Homolog B1 and Cyclin-Dependent Kinase 4 ... Gossypin as a Novel Selective Dual Inhibitor of v-raf Murine Sarcoma Viral Oncogene Homolog B1 and Cyclin-Dependent Kinase 4 ... Gossypin as a Novel Selective Dual Inhibitor of v-raf Murine Sarcoma Viral Oncogene Homolog B1 and Cyclin-Dependent Kinase 4 ...
ABSTRACTCell cycle progression requires precise expression and activation of several cyclins and cyclin-dependent kinases. ... Cdc2 and cyclin B1 were ubiquitinated by GA. MG132 abrogated the GA-induced decrease of Cdc2 and cyclin B1 indicating that ... In conclusion, GA controls the stability of Cdc2 and cyclin B1 in glioblastomas cell species-dependently. Cdc2 and cyclin B1 ... GA decreased the expression of Cdc2 and cyclin B1 in U87MG cells. And phosphorylated Cdc2 decreased along with Cdc2 in the GA- ...
a) Antibody titers to cyclin B1 in ELISA were expressed as optical density (OD) units. The mean + 3SD of normal human sera are ... to cyclin B1 before and after absorption in nitrocellulose membrane strips blotted with purified recombinant cyclin B1 protein ... to cyclin B1 before and after absorption in nitrocellulose membrane strips blotted with purified recombinant cyclin B1 protein ... Autoantibodies to cyclin B1 were detected in 31.0% of sera from randomly selected patients with PCa versus 4.8% in sera with ...
ATP-Binding Cassette B1 Transports Seliciclib (R-Roscovitine), a Cyclin-Dependent Kinase Inhibitor. Zsuzsanna Rajnai, Dóra Méhn ... ATP-Binding Cassette B1 Transports Seliciclib (R-Roscovitine), a Cyclin-Dependent Kinase Inhibitor. Zsuzsanna Rajnai, Dóra Méhn ... ATP-Binding Cassette B1 Transports Seliciclib (R-Roscovitine), a Cyclin-Dependent Kinase Inhibitor. Zsuzsanna Rajnai, Dóra Méhn ... ATP-Binding Cassette B1 Transports Seliciclib (R-Roscovitine), a Cyclin-Dependent Kinase Inhibitor ...
CCNB1; cyclin B1; CCNB; G2/mitotic-specific cyclin-B1; OTTHUMP00000123478; OTTHUMP00000221981; OTTHUMP00000223023; G2/mitotic- ... Recombinant Human Cyclin B1, His-tagged. E. coli. Human. His. +Inquiry. CCNB1-2983M. Recombinant Mouse CCNB1 Protein. Mammalian ...
  • For example, an increase in expression of cyclin B1/cdc2 is significantly higher in breast tumor tissue and shown to increase lymph node metastasis in breast cancer. (wikipedia.org)
  • Results provide evidence that cdc2/cyclin B1 kinase activation was synchronous with the initial appearance of cytoskeletal lesions in mouse with Niemann-Pick disease type C. (nih.gov)
  • Title: Aberrant activation of Cdc2/cyclin B1 is involved in initiation of cytoskeletal pathology in murine Niemann-Pick disease type C. (nih.gov)
  • Cyclin B1 is the regulatory subunit of the cdc2 kinase and is a protein required for mitotic initiation. (pnas.org)
  • Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). (thermofisher.com)
  • This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). (scbt.com)
  • Evidence has established that B type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. (scbt.com)
  • A high expression of CCNB1 (cyclin B1), CDC2, CDKN3A, CKS1B, ANP32E, and KIAA0101, but not of the proliferation-related antigen Ki-67, was associated with better survival rate in a univariate analysis. (lu.se)
  • To determine the effects of progesterone on the proliferation, cell cycle progression and apoptosis of hECs and to test if cyclin B1 is involved in these effects, progesterone and/or Alsterpaullone (Alp, a specific inhibitor of Cyclin B1/Cdc2) were added to primary hECs. (biomedsearch.com)
  • GA decreased the expression of Cdc2 and cyclin B1 in U87MG cells. (ovid.com)
  • In U251MG cells, the cell cycle was arrested at M phase in addition to G2 by GA. Next, we analyzed the mechanism of the GA-induced regulation of Cdc2 and cyclin B1 in U87MG cells. (ovid.com)
  • Cdc2 and cyclin B1 were ubiquitinated by GA. MG132 abrogated the GA-induced decrease of Cdc2 and cyclin B1 indicating that these proteins were degraded by proteasomes. (ovid.com)
  • In conclusion, GA controls the stability of Cdc2 and cyclin B1 in glioblastomas cell species-dependently. (ovid.com)
  • Cdc2 and cyclin B1 might be responsible for the different responses of glioblastoma cell lines to GA. (ovid.com)
  • Activation of the Cyclin B/Cdc2 kinase complex triggers entry into mitosis in all eukaryotic cells. (duke.edu)
  • There was no change in the protein expression of cyclins A or E. Therefore, the effect of huanglian on inhibiting tumor growth seems to be mediated by the selective suppression of cyclin B1, which results in the inhibition of cdc2 kinase activity. (aspetjournals.org)
  • The initial activation of the CCNB:CDK1 (cyclin B1:Cdc2 and cyclin-B2:Cdc2) complexes occurs in the cytoplasm in prophase (Jackman et al. (reactome.org)
  • Cyclin B1 is the regulatory subunit of the cdc2 serine/threonine kinase, and accumulation of cyclin B1 in late G2 phase of the cell cycle is a prerequisite for mitotic initiation in mammalian cells. (ashpublications.org)
  • The cyclin B protein and its binding partner, the cdc2 serine/threonine kinase, regulate mitotic initiation in vertebrate cells. (ashpublications.org)
  • 1-3 The cyclin B/cdc2 heterodimer induces mitosis by phosphorylating and activating enzymes regulating chromatin condensation, nuclear membrane breakdown, and mitosis-specific microtubule reorganization. (ashpublications.org)
  • 4 Activation of cdc2 requires changes in cdc2 phosphorylation 5 as well as association with cyclin B. 2 There are two human and mouse B-type cyclins, B1 and B2. (ashpublications.org)
  • Cyclin B1 and cdc2 are also apoptotic regulators. (ashpublications.org)
  • 12 , 13 The apoptosis induced by granzyme B, taxol, Fas, camptothecin, Nerve Growth Factor (NGF) withdrawal, human immunodeficiency virus infection, and T-cell activation are all associated with cdc2 activation and/or accumulation of cyclin B1 protein. (ashpublications.org)
  • 14-20 Thus, cdc2 and cyclin B1 are likely to be involved in multiple apoptotic pathways. (ashpublications.org)
  • Mitosis is triggered in vertebrate cells from the cyclin B1-Cdc2 organic. (biomarketx.net)
  • All purified cyclin B1 protein were fully useful as activators of Cdc2 cyclin B1 (11 34 We ready mutant variations of individual cyclin B1 where these residues had been transformed either to VX-689 Glu (cyclin VX-689 B1E) or Ala (cyclin B1A). (biomarketx.net)
  • We also discovered that a 16-flip more than untagged cyclin B1 (4 VX-689 μM) totally inhibited the nuclear deposition of Myc-epitope-tagged cyclin B1-Cdc2 complexes (data not really shown). (biomarketx.net)
  • We therefore explored the possibility that the cytosol component required for cyclin B1-Cdc2 import in our system is importin β. (biomarketx.net)
  • and cyclin B1-Cdc2 complexes (Fig. (biomarketx.net)
  • Cyclins, when bound with the dependent kinases , such as the p34 / cdc2 / cdk1 protein, form the maturation-promoting factor . (wikipedia.org)
  • Enhanced radiosensitivity by cyclin G1 was correlated with increased cyclin B1, CDC2/cyclin B1 complex, and MPM2. (elsevier.com)
  • In order to determine the regulatory mechanism and effect of different HPV subtypes, we performed functional assays on cdc2, cyclinB1 and HuR in human uterine cervical samples. (elsevier.com)
  • western blotting for cyclin B1, cdc2 and phospho-cdc2 (Y15 and T161), immunoprecipitation for cdc2, a nuclear extraction fractional assay, and RT-PCR for cyclin B1. (elsevier.com)
  • The expression of cyclin B1 was found to be dependent on HPV type, and was particularly overexpressed in high-risk types, whereas cdc2 was ubiquitously expressed irrespective of HPV type. (elsevier.com)
  • Phospho-cdc2 and cyclin B1, however, were most intense in HPV18 infected cervical samples. (elsevier.com)
  • This study demonstrates that HPV-18 decreases the fidelity of mitotic checkpoints and increases cdc2-associated histone H1 kinase activity relative to control populations, and further shows that the G2 checkpoint is aberrant by virtue of the stabilization of cyclin B1 mRNA through the upregulation of HuR protein and the functional form of cdc2, especially in cases with HPV 18 infected cervical lesions. (elsevier.com)
  • To examine the therapeutic utility of this method, we transferred antisense phosphorothioate oligos against cyclin B1- and CDC2 kinase-encoding genes into balloon-injured rat carotid artery as a potential therapy for experimental restenosis. (duke.edu)
  • Two weeks after transfection, antisense oligo treatment directed against either CDC2 kinase or cyclin B1 resulted in a partial, but significant, inhibition in neointima formation. (duke.edu)
  • Interestingly, co-transfection of antisense oligos against CDC2 kinase and cyclin B resulted in further inhibition of neointima formation, as compared to blockade of either gene target alone. (duke.edu)
  • and (ii) HVJ-mediated administration of antisense CDC2 kinase and cyclin B1 oligos produces a sustained inhibition of neointima formation after balloon angioplasty. (duke.edu)
  • Here we show that the functional human ortholog of Greatwall protein kinase (Gwl) is the microtubule-associated serine/threonine kinase-like protein, MAST-L. This kinase promotes mitotic entry and maintenance in human cells by inhibiting protein phosphatase 2A (PP2A), a phosphatase that dephosphorylates cyclin B-Cdc2 substrates. (pnas.org)
  • More complete depletion correlates with the premature dephosphorylation of cyclin B-Cdc2 substrates, inactivation of the SAC, and subsequent exit from mitosis with severe cytokinesis defects. (pnas.org)
  • These results suggest that the balance between cyclin B-Cdc2 and PP2A must be tightly regulated for correct mitotic entry and exit and that Gwl is crucial for mediating this regulation in somatic human cells. (pnas.org)
  • In eukaryotic cells, the mitotic state is maintained by the mitotic kinase cyclin B-Cdc2. (pnas.org)
  • Historically, mitotic entry and exit was thought to be the direct consequence of cyclin B-Cdc2 activation and inactivation, respectively ( 1 ). (pnas.org)
  • Specifically, recent evidence indicates that protein phosphatase 2A (PP2A) is responsible for dephosphorylation of cyclin B-Cdc2 substrates and that the regulation of this dephosphorylation is required in mitotic entry and exit ( 3 ). (pnas.org)
  • This finding suggests a previously unexplored model in which the balance between cyclin B-Cdc2 and PP2A controls mitotic entry and exit. (pnas.org)
  • Thus, during G2 PP2A activity is high and cyclin B-Cdc2 activity low, thereby preventing phosphorylation of mitotic substrates, whereas at mitotic entry the balance flips, allowing entry into mitosis. (pnas.org)
  • The mechanisms controlling cyclin B-Cdc2 activity have been largely described ( 4 ). (pnas.org)
  • Briefly, cyclin B-Cdc2 is inhibited during G2 by inhibitory phosphorylations on threonine 14 and tyrosine 15 by Wee1 and Myt1 kinases. (pnas.org)
  • Finally, at mitotic exit cyclin B-Cdc2 is inhibited by the ubiquitin-dependent degradation of its regulatory subunit cyclin B ( 5 ). (pnas.org)
  • Unlike cyclin B-Cdc2 regulation, very little is known about the mechanisms controlling PP2A activity during mitosis, and therefore our understanding of G2 and mitosis is incomplete. (pnas.org)
  • Work done in Xenopus egg extracts suggested that Gwl promoted mitotic entry by controlling the auto-amplification loop of cyclin B/Cdc2 ( 8 , 9 ). (pnas.org)
  • However, it has been recently demonstrated that the main role of this kinase is not the regulation of cyclin B-Cdc2 activity but the inhibition of PP2A, the phosphatase that de-phosphorylates cyclin B-Cdc2 substrates ( 10 , 11 ). (pnas.org)
  • This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. (novusbio.com)
  • Vanadate also increased p21 and Chk1 levels and reduced Cdc25C expression, leading to phosphorylation of Cdc2 and a slight increase in cyclin B1 expression as analyzed by Western blot. (cdc.gov)
  • Several regulatory pathways are involved: (1) activation of p21, (2) an increase of Chk1 expression and inhibition of Cdc25C, which results in phosphorylation of Cdc2 and possible inactivation of cyclin B1/Cdc2 complex. (cdc.gov)
  • Cyclin A interacts with CDK2 for S phase progression, and cyclin-A/B associates with CDC2 for entry into M phase [ 5 , 6 ]. (omicsonline.org)
  • It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. (genecards.org)
  • The following product was used in this experiment: Cyclin B1 Polyclonal Antibody from Thermo Fisher Scientific, catalog # 55004-1-AP. (thermofisher.com)
  • Cyclin B1 Monoclonal antibody specifically detects Cyclin B1 in Human, Mouse samples. (fishersci.com)
  • The following product was used in this experiment: Phospho-Cyclin B1 (Ser126) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-104795, RRID AB_2816268. (thermofisher.com)
  • Western blot analysis using Anti-Cyclin B1 antibody shows detection of human Cyclin B1 present in asynchronous HN30 cell lysates. (antibodies-online.com)
  • anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. (antibodies-online.com)
  • Cyclin B1 antibody detects Cyclin B1 protein at cytoplasm on mouse ovary by immunohistochemical analysis. (genetex.com)
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 cyclin B1 Antibody (V152) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装 (scbt.com)
  • Can cyclin B1 (V152): sc-53236 monoclonal antibody be used in IF or IHC with mouse tissue or cells? (scbt.com)
  • This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. (biotium.com)
  • a) Antibody titers to cyclin B1 in ELISA were expressed as optical density (OD) units. (nih.gov)
  • b) Western blotting analysis shows antibody reactivity to cyclin B1 before and after absorption in nitrocellulose membrane strips blotted with purified recombinant cyclin B1 protein. (nih.gov)
  • Knockout Tested Rabbit recombinant monoclonal Cyclin B1 antibody [Y106]. (lyceesdefumel.com)
  • Results and Conclusions: Cyclin B1 elicits helper T cell-dependent antibody responses in vivo. (lyceesdefumel.com)
  • Tumors with higher level of cyclin B1 expression elicit higher anti-cyclin B1 antibody levels. (lyceesdefumel.com)
  • Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E) contains anti-Cyclin E1 antibody,anti-Cyclin B1 antibody [V152],anti-Histone H3 phospho (Ser10) antibody. (lyceesdefumel.com)
  • Monoclonal Antibody for studying Cyclin B1 in the Cell Cycle / Checkpoint research area. (lyceesdefumel.com)
  • There are currently no images for Cyclin C Antibody (NB120-2950). (novusbio.com)
  • Western Blot: Cyclin A1 Antibody [NBP1-02902] - Cyclin A1 western with U2OS cells. (novusbio.com)
  • Immunohistochemistry-Paraffin: Cyclin A1 Antibody [NBP1-02902] - Human testis after heat-induced antigen retrieval. (novusbio.com)
  • Cyclin B1 is a regulatory protein involved in mitosis. (wikipedia.org)
  • Cyclin B1 contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. (wikipedia.org)
  • Cyclin B1-Cdk1 is involved in the early events of mitosis, such as chromosome condensation, nuclear envelope breakdown, and spindle pole assembly. (wikipedia.org)
  • Once activated, cyclin B1-Cdk1 promotes several of the events of early mitosis. (wikipedia.org)
  • Just prior to mitosis, a large amount of cyclin B1 is present in the cell, but it is inactive due to phosphorylation of Cdk1 by the Wee1 kinase. (wikipedia.org)
  • Once cyclin B1-Cdk1 is activated, it remains stably active for the rest of mitosis. (wikipedia.org)
  • Before mitosis almost all cyclin B1 in the cell is located in the cytoplasm, but in late prophase it relocates to the nucleus. (wikipedia.org)
  • At the end of mitosis, cyclin B1 is targeted for degradation by the APC through its APC localization sequence, permitting the cell to exit mitosis. (wikipedia.org)
  • The results show that Cyclin B1 is essential for mitosis in mammalian cells and that its spatial localization has a crucial role in triggering mitosis at the correct time, even when Cdk1 can be regulated by the antagonistic Wee1-Myt1-Cdc25 pathways. (nih.gov)
  • Title: Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis. (nih.gov)
  • Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. (thermofisher.com)
  • Cyclin B1 binds CDK1, a cyclin-dependent kinase catalytic subunit, forming a complex that orchestrates mitosis through phosphorylation of key proteins. (nih.gov)
  • These findings suggest that Cdk1-cyclin B1-mediated phosphorylation of TMAP is important for and contributes to proper regulation of microtubule dynamics and establishment of functional bipolar spindles during mitosis. (scialert.net)
  • Cyclin B1 localization changes dramatically during the cell cycle, precipitously transiting from the cytoplasm to the nucleus at the beginning of mitosis. (duke.edu)
  • In mitosis, Cyclin B1 undergoes phosphorylation at several sites, a subset of which have been proposed to play a role in Cyclin B1 accumulation in the nucleus. (duke.edu)
  • The cyclin B1-Cdk1 complex is the master regulator of mitosis. (rupress.org)
  • At unattached kinetochores, spindle checkpoint components catalyze the formation of an inhibitor of the anaphase-promoting complex/cyclosome (APC/C), the ubiquitin ligase that promotes sister chromatid separation and cyclin B degradation to trigger anaphase onset and exit from mitosis ( Barford, 2011 ). (rupress.org)
  • We found that Cyclin B1 must be exported from the nucleus for the cell to prevent premature entry to mitosis, and retaining Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis. (cam.ac.uk)
  • 6 Conversely, homozygous deletion of cyclin B1 leads to death in utero, 6 consistent with the idea that cyclin B1 is likely the primary regulator of mammalian mitosis. (ashpublications.org)
  • 7 In Xenopus oocytes, the amount of cyclin B protein is 20- to 30-fold higher in late G2 than in G1, and a threshold level of cyclin B must be reached before mitosis can proceed. (ashpublications.org)
  • In mammalian cells, the expression level of the cyclin B1 gene plays a critical role in the progression through mitosis. (embopress.org)
  • Here we demonstrate that the transcriptional activity of the human cyclin B1 promoter, as well as the rate of gene transcription, is high during mitosis. (embopress.org)
  • Consistent with this, we show that the cyclin B1 promoter is occupied and bound to NF‐Y during mitosis in vivo . (embopress.org)
  • However, it has not been demonstrated yet whether the cyclin B1 promoter retains transcriptional activity during mitosis. (embopress.org)
  • Indeed, we show that the cyclin B1 promoter is accessible to restriction endonucleases and interacts with transcription factors during mitosis in vivo . (embopress.org)
  • In addition, we demonstrate by formaldehyde cross‐linking and immunoprecipitation assays that NF‐Y is bound to the cyclin B1 promoter during mitosis. (embopress.org)
  • Our results show that the RNA pol II‐transcribed gene cyclin B1 is actively transcribed during mitosis where the promoter retains open conformation and factor occupancy. (embopress.org)
  • The levels of S cyclins remain high, not only throughout S phase, but through G2 and early mitosis as well to promote early events in mitosis. (wikipedia.org)
  • M cyclin concentrations rise as the cell begins to enter mitosis and the concentrations peak at metaphase. (wikipedia.org)
  • The destruction of M cyclins during metaphase and anaphase, after the Spindle Assembly Checkpoint is satisfied, causes the exit of mitosis and cytokinesis. (wikipedia.org)
  • For example, the amino-terminal regions of S and M cyclins contain short destruction-box motifs that target these proteins for proteolysis in mitosis. (wikipedia.org)
  • Activation of CDK1 protein kinase and formation of Cyclin B1-CDK1 complex is an obligate step for access into mitosis and hence is under considerable regulations [14,15]. (cancerrealitycheck.com)
  • Dr. Porter's lab has characterized how Tuberin is able to regulate the G2/M transition of the cell cycle by binding and regulating the localization of CyclinB1, the protein that allows for the transition of the cell into mitosis. (uwindsor.ca)
  • Phosphorylation of the lamins by cyclin B1-Cdk1 causes them to dissociate, compromising the structural integrity of the nuclear envelope so that it breaks down. (wikipedia.org)
  • A possible trigger for activation is phosphorylation by cyclin A-Cdk, which functions before cyclin B1-Cdk in the cell cycle. (wikipedia.org)
  • This is regulated by the phosphorylation of cyclin B1, in contrast to phosphorylation of Cdk1 regulating the activity of the complex. (wikipedia.org)
  • Phosphorylation of cyclin B1 causes it to be imported to the nucleus, and phosphorylation also prevents export from the nucleus by blocking the nuclear export signal. (wikipedia.org)
  • AURKA induced phosphorylation and recruitment of CDC25B to MTOCs prior to p-Cyclin B1-Ser123, and this sequential regulation is essential for the commitment of the oocytes to resume meiosis. (nih.gov)
  • In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. (fishersci.com)
  • A synthesized peptide derived from human Cyclin B1 around the phosphorylation site of Ser126. (thermofisher.com)
  • The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. (scbt.com)
  • We conclude that cyclin B1 accumulates at kinetochores during prometaphase, where it contributes to the correct attachment of microtubules to kinetochores and efficient alignment of the chromosomes, most likely through localized phosphorylation of specific substrates by cyclin B1-CDK1. (nih.gov)
  • thus, we propose that Cyclin B1 phosphorylation at the G2/M transition prevents its interaction with CRM1, thereby reducing nuclear export and facilitating nuclear accumulation. (duke.edu)
  • At the mechanistic level, TIS21 inhibited FoxM1 phosphorylation, a required modification for its activation, by reducing cyclin B1-cdk1 activity, examined by in vitro kinase assay and FoxM1 mutant analyses. (ajou.ac.kr)
  • These observations were further confirmed in vivo by the reciprocal control of TIS21 expression and FoxM1 phosphorylation in the diethylnitrosamine-induced HCCs and TIS21(-/-) mouse embryonic fibroblast (MEF), in addition to increased expression of cyclin B1 and cdk1 activity. (ajou.ac.kr)
  • Investigation of the pUL97-cyclin T1 interaction in an ATP consumption assay strongly suggested phosphorylation of pUL97 by the CDK9/cyclin T1 complex in a substrate concentration-dependent manner. (mdpi.com)
  • A cyclin forms a complex with Cdk, which begins to activate but the complete activation requires phosphorylation, as well. (wikipedia.org)
  • Activity of CDK1 is usually controlled through association with Cyclin B1, reversible phosphorylation [16] and subcellular localizations [14,17]. (cancerrealitycheck.com)
  • Catalase, a specific antioxidant for H2O2, decreased vanadate-induced expression of p21 and Chk1, reduced phosphorylation of Cdc2Tyr15, and decreased cyclin B1 levels. (cdc.gov)
  • Encodes a member of a plant specific family of cyclin dependent kinases. (nih.gov)
  • p53 inhibits G 1 /S transition in cells exposed to DNA-damaging agents by causing accumulation of p21 CIP1/WAF1 ( 6 , 15 ), a protein that binds to and inactivates the cyclin-dependent kinases necessary for initiating DNA synthesis ( 16 ). (pnas.org)
  • Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). (thermofisher.com)
  • Cell cycle progression requires precise expression and activation of several cyclins and cyclin-dependent kinases. (ovid.com)
  • These results indicate that traditional Chinese herbs may represent a new source of agents designed for selective inhibition of cyclin dependent kinases in cancer therapy. (aspetjournals.org)
  • P27, an inhibitor of cyclin-dependent kinases. (lyceesdefumel.com)
  • Cyclins are characterized by a dramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. (rockland-inc.com)
  • We show that these kinases can readily be distinguished by such inhibitors when cyclin-free, but not when cyclin-bound. (rcsb.org)
  • Cyclins function as regulators of CDK kinases. (novusbio.com)
  • Cyclin-dependent kinases (CDKs) at the G1-S and G2-M checkpoints are positively regulated by cyclins and negatively regulated by CDK inhibitors. (omicsonline.org)
  • The cell cycle is governed by cyclin-dependent kinases (CDKs), which are positively regulated by cyclins and negatively regulated by CDK inhibitors (CKIs) [ 3 ]. (omicsonline.org)
  • Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated. (wikipedia.org)
  • Another important function of the cyclin B1-Cdk1 complex is to break down the nuclear envelope. (wikipedia.org)
  • Another mechanism by which cyclin B1-Cdk1 activity is regulated is through subcellular localization. (wikipedia.org)
  • Cyclin B1 is phosphorylated by Polo kinase and Cdk1, again setting up a positive feedback loop that commits cyclin B1-Cdk1 to its fate. (wikipedia.org)
  • Cyclin B1 has been shown to interact with Cdk1, GADD45A and RALBP1. (wikipedia.org)
  • Cyclin B1 regulates both the activation of CDK1 and its subcellular localization, which may be critical for substrate selection. (nih.gov)
  • Cyclin B1 is then transported from each kinetochore as microtubule attachment is completed, and this relocalization may redirect the activity of cyclin B1-CDK1 and contribute to inactivation of the spindle assembly checkpoint. (nih.gov)
  • Among these, Thr-622 was specifically phosphorylated by Cdk1-cyclin B1 both in vitro and in vivo . (scialert.net)
  • Once cyclin B1 accumulates to a threshold level during G2, full activation of its kinase partner Cdk1 transforms the cell from an interphase to a mitotic state. (rupress.org)
  • Activated cyclin B1-Cdk1 phosphorylates a number of cellular targets in order to trigger diverse events such as chromosome condensation, nuclear envelope breakdown, mitotic spindle formation, and chromosome segregation. (rupress.org)
  • None of the mammalian A-, D-, or E-type cyclins are required in development until implantation, and only Cdk1 is essential for early cell divisions. (cam.ac.uk)
  • This is the earliest arrest of any Cyclin known and places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. (cam.ac.uk)
  • Western-blot showed G2M arrest caused by ESRRA silencing was via CDC25C-CDK1-Cyclin B1 pathway. (ijbs.com)
  • ESRRA/DSN1/CDC25C-CDK1-Cyclin B1 is of great importance in GC development. (ijbs.com)
  • Unusual expression of Cyclin CDK1 and B1 continues to be discovered in a variety of tumors. (cancerrealitycheck.com)
  • However, to your understanding no prior research has investigated Cyclin B1 and CDK1 in vulvar malignancy. (cancerrealitycheck.com)
  • Cyclin B1-CDK1 complex plays an important role in G2/M cell cycle. (cancerrealitycheck.com)
  • For Cyclin B1, the residues Ser126 and Ser128 around the N-terminal are one of the primary ones that are autophosphorylated by Cyclin B1-CDK1 organic [17], in order that pCyclin B1Ser126 is undoubtedly the equivalent type of energetic Cyclin B1. (cancerrealitycheck.com)
  • In a few of these reviews the altered degree of Cyclin B1 and/or CDK1 appearance indicated an unhealthy outcome of sufferers [25,27]. (cancerrealitycheck.com)
  • Restoring p53 function in human melanoma cells by inhibiting MDM2 and cyclin B1/CDK1-phosphorylated nuclear iASPP. (ox.ac.uk)
  • Here, we identify that cyclin B1/CDK1-phosphorylates iASPP, which leads to the inhibition of iASPP dimerization, promotion of iASPP monomer nuclear entry, and exposure of its p53 binding sites, leading to increased p53 inhibition. (ox.ac.uk)
  • causes a G2 cell cycle arrest by inhibiting CDK1 activity through the regulation of cyclin B1, GADD45A, and BTG2. (viraquest.com)
  • We have used biophysical measurements and X-ray crystallography to investigate the ATP-competitive inhibitor binding properties of cyclin-free and cyclin-bound CDK1 and CDK2. (rcsb.org)
  • We conclude that there is a subtle but profound difference between the conformational energy landscapes of cyclin-free CDK1 and CDK2. (rcsb.org)
  • Our results show that p38 is activated in response to hydroxyurea treatment and collaborates with Chk1 to prevent mitotic entry in non-transformed cell lines by maintaining cyclin B1/Cdk1 complexes inactive. (aacrjournals.org)
  • Because Cdc25 phosphatase family activates cyclin B1/Cdk1, which is essential for mitotic entry, the cell cycle is finally arrested at the G 2 -M transition. (aacrjournals.org)
  • Western blot analysis showed that taurine significantly limited the ionizing radiation-induced down-regulation of CyclinB1 and CDK1, and suppressed activation of Fas/FasL system pathway. (bireme.br)
  • The mRNA expression of cyclin B1 was not changed after huanglian treatment. (aspetjournals.org)
  • cyclin B1 mRNA at the animal polar cytoplasm of immature zebrafish oocytes is translationally repressed, and its activation is temporally regulated during maturation. (hokudai.ac.jp)
  • Real-time imaging of the site and timing of translation showed that mRNAs containing the full-length cyclin B1 sequence precisely mimic the localization and translation of endogenous cyclin B1 mRNA. (hokudai.ac.jp)
  • However, mRNAs containing cyclin B1 3' untranslated region but lacking open reading frame (ORF) underwent abnormal localization and precocious translational activation, indicating the significance of the ORF in translational control of cyclin B1 mRNA. (hokudai.ac.jp)
  • Immature mouse oocytes were cultured in vitro with a known dynein ATPase activity inhibitor- sodium orthovanadate (SOV) to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the changes of cyclin B1 mRNA level. (bvsalud.org)
  • In time - course experiment, the opposite changes of cyclin B1 mRNA level in oocytes between SOV and control groups were observed. (bvsalud.org)
  • There were no significant differences between the size categories of oocytes at collection in total RNA content, total protein content and Cyclin B1 mRNA. (uab.cat)
  • We compared the translational regulation and polyadenylation of the cyclin B1 mRNA during zebrafish and Xenopus oocyte maturation. (biomedcentral.com)
  • Polyadenylation and translational activation of cyclin B1 mRNA is well characterized during Xenopus oocyte maturation. (biomedcentral.com)
  • Specifically, Xenopus cyclin B1 mRNA is polyadenylated and translationally activated during oocyte maturation by proteins that recognize the conserved AAUAAA hexanucleotide and U-rich Cytoplasmic Polyadenylation Elements (CPEs) within cyclin B1 mRNA's 3' U n T ranslated R egion (3' UTR ). (biomedcentral.com)
  • The zebrafish cyclin B1 mRNA was polyadenylated during zebrafish oocyte maturation. (biomedcentral.com)
  • Furthermore, the zebrafish cyclin B1 mRNA's 3'UTR was sufficient to stimulate translation of a reporter mRNA during zebrafish oocyte maturation. (biomedcentral.com)
  • However, in contrast to AAUAAA, the positions and sequences of the functionally defined CPEs were poorly conserved between Xenopus and zebrafish cyclin B1 mRNA 3'UTRs. (biomedcentral.com)
  • To determine whether these differences were relevant to translation efficiency, we analyzed the translational activity of reporter mRNAs containing either the zebrafish or Xenopus cyclin B1 mRNA 3'UTRs during both zebrafish and Xenopus oocyte maturation. (biomedcentral.com)
  • Although the factors that regulate translation of maternal mRNAs are highly conserved, the target sequences and overall sequence architecture within the 3'UTR of the cyclin B1 mRNA have diverged to affect translational efficiency, perhaps to optimize levels of cyclin B1 protein required by these different species during their earliest embryonic cell divisions. (biomedcentral.com)
  • In this report we examine translational regulation of the maternal cyclin B1 mRNA during zebrafish oocyte maturation. (biomedcentral.com)
  • In situ hybridization confirmed that this EGFP expression matched the cyclin B1 mRNA expression pattern. (molvis.org)
  • G2/mitotic-specific cyclin-B1 is a protein that in humans is encoded by the CCNB1 gene. (wikipedia.org)
  • The ability of p53 to control mitotic initiation by regulating intracellular cyclin B1 levels suggests that the cyclin B-dependent G 2 checkpoint has a role in preventing neoplastic transformation. (pnas.org)
  • To study G 2 regulation by p53, we have established a human cell line, Ts-SKOV3, that stably expresses a temperature-sensitive p53 allele and undergoes G 2 arrest at 32°C. Using this cell line we have found that p53 arrests cell cycle in G 2 by lowering intracellular levels of cyclin B1, a protein absolutely required for mitotic initiation. (pnas.org)
  • This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. (fishersci.com)
  • In a recent analogy, an interaction between the master mitotic regulator cyclin B1 and the spindle checkpoint component Mad1 was independently described by three groups who propose strikingly different functions for this interaction. (rupress.org)
  • Although cyclin A2 and cyclin B1 are both targeted for degradation by the APC/C, during the spindle assembly checkpoint (SAC), the mitotic checkpoint complex (MCC) represses APC/C's activity towards. (lyceesdefumel.com)
  • Regulation of intracellular cyclin B levels is one of the mechanisms controlling mitotic initiation. (ashpublications.org)
  • Indeed, the cyclin B1 promoter maintains an open chromatin configuration at the mitotic stage. (embopress.org)
  • In mammalian cells, the G 2 /M transition is controlled by the mitotic cyclins A and B1 ( Jackman and Pines, 1997 ). (embopress.org)
  • Here we provide evidence for cyclin B1 transcription at the mitotic stage in mammalian cells. (embopress.org)
  • Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. (rockland-inc.com)
  • Cell changes in the cell cycle like the assembly of mitotic spindles and alignment of sister-chromatids along the spindles are induced by M cyclin- Cdk complexes. (wikipedia.org)
  • Depletion of cyclin G1 by interference RNA revealed that cyclin G1 regulated transcription of cyclin B1 in a p53-independent manner, and confirmed that the increased mitotic cells and cell death by cyclin G1 were dependent upon cyclin B1. (elsevier.com)
  • Apart from this fast response, G 2 DNA damage also induces changes in gene expression that finally maintain mitotic entry arrest, such as p53-induced Gadd45, p21, and 14-3-3σ expression or p53-dependent and p53-independent down-regulation of cyclin B1 or Plk1 ( 7 - 9 ). (aacrjournals.org)
  • Mxi1 inhibits the proliferation of U87 glioma cells through down-regulation of cyclin B1 gene expression. (uniprot.org)
  • Thus, our findings indicate that Mxi1 can act as a tumour suppressor in human glioblastomas through a molecular mechanism involving the transcriptional down-regulation of cyclin B1 gene expression. (uniprot.org)
  • Cyclin B2 is not essential for mouse development, and mice homozygous for a targeted deletion of the cyclin B2 gene are viable, fertile, and develop normally. (ashpublications.org)
  • Cyclin D1 (also known as G1/S-specific cyclin D1, PRAD1 oncogene, BCL-1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family. (rockland-inc.com)
  • [5] ) The oscillations of the cyclins, namely fluctuations in cyclin gene expression and destruction by the ubiquitin mediated proteasome pathway, induce oscillations in Cdk activity to drive the cell cycle. (wikipedia.org)
  • We cloned the 1.5 kb promoter region of the zebrafish cyclin B1 ( ccnb1 ) gene upstream of the EGFP gene in the Tol2 vector, which was used to generate the stable Tg ( ccnb1:EGFP ) nt18 transgenic zebrafish line. (molvis.org)
  • The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. (novusbio.com)
  • The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. (novusbio.com)
  • Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. (rockland-inc.com)
  • Cyclin A/ Cdk2 is inhibited by the complex p21CIP. (rockland-inc.com)
  • Cyclin E binds to the G1 phase Cdk2, which is required for the transition from G1 to S phase of the cell cycle that determines cell division. (rockland-inc.com)
  • The Cyclin E/CDK2 complex phosphorylates p27Kip1, tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase. (rockland-inc.com)
  • Cyclin E interacts with CDK2 for the G1-S transition. (omicsonline.org)
  • Also dephosphorylates CDK2 in complex with cyclin E, in vitro. (genecards.org)
  • A single bivalent efficiently inhibits cyclin B1 degradation and polar body extrusion in mouse oocytes indicating robust SAC during female meiosis I. Hoffmann S, et al . (nih.gov)
  • Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosis. (nih.gov)
  • MPF activity is stable in eggs, and a sperm-triggered Ca2+ signal is needed to promote cyclin degradation [2]. (ncl.ac.uk)
  • However, APC/C-dependent cyclin degradation was elevated 6-fold when sperm raised cytosolic Ca2+ levels above 600 nM. (ncl.ac.uk)
  • For continued cyclin degradation at basal Ca2+ levels, multiple spikes were needed. (ncl.ac.uk)
  • SAC satisfaction is reversible, however, as addition of taxol during metaphase stops cyclin B1 degradation by the APC/C. We now show that targeting MAD1 to kinetochores during metaphase is sufficient to reestablish SAC activity after initial silencing. (springer.com)
  • Using rapamycin-induced heterodimerization of FKBP-MAD1 to FRB-MIS12 and live monitoring of cyclin B1 degradation, we show that timed relocalization of MAD1 during metaphase can stop cyclin B1 degradation without affecting chromosome-spindle attachments. (springer.com)
  • Immunity against cyclin B1 tumor antigen delays development of spontaneous cyclin B1-positive tumors in p53 (-/-) mice. (nih.gov)
  • In the further analysis, 31.4% of sera from PCa patients at the early stage contained anti-cyclin B1 autoantibody, and even 29.4% of patients who had normal prostate-specific antigen (PSA) levels in their serum samples were observed anti-cyclin B1 positive. (nih.gov)
  • All of sera with OD value over the cut-off in ELISA were confirmed by Western blotting using purified cyclin B1 protein as antigen. (nih.gov)
  • Starting from serum antibodies to eight known breast cancer antigens, we first identified four serum antibodies, HER2/neu, p53, carcinoembryonic antigen (CEA), and cyclin B1, which are significantly increased in the sera collected from patients with breast cancer at the time of treatment. (aacrjournals.org)
  • Expression of hepatic cyclin B1 and proliferating cell nuclear antigen were evaluated by Western Blot and liver injury was assessed by ALT and histology. (biomedcentral.com)
  • In the absence of NKT cells hepatic proliferating cell nuclear antigen and cyclin B1 decreased in mice injected with Concanvalin A before partial hepatectomy. (biomedcentral.com)
  • Cyclin B1 turnover and the mechanism causing insensitivity of fully grown mouse oocytes to cycloheximide inhibition of meiotic resumption. (nih.gov)
  • Mechanisms of cyclin B1 translation in oocytes were analyzed using transgenic zebrafish in which reporter mRNAs are produced from transgenes introduced into the genome through transcription in the nucleus followed by transport to the cytoplasm, as in endogenous mRNAs. (hokudai.ac.jp)
  • Furthermore, conjugates between PTD of TCTP and cyclin B1 siRNAs internalized into the cytoplasm of oocytes and downregulated cyclin B1 level. (nature.com)
  • Cyclin B1 levels were elevated ~5-fold in Fzr1 Δ/Δ oocytes, whereas securin and CDC25B, two other APC/C FZR1 substrates, were unchanged. (biologists.org)
  • Cyclin B1 overexpression can mimic the effects of Fzr1 loss on GV arrest and here we show that cyclin B1 knockdown in Fzr1 Δ/Δ oocytes affects the timing of meiotic resumption. (biologists.org)
  • Thus, APC/C FZR1 activity is required to repress cyclin B1 levels in oocytes during prophase I arrest in the ovary, thereby maintaining meiotic quiescence until hormonal cues trigger resumption. (biologists.org)
  • Dynein inhibitor might delay oocytes meiosis process, and cause ectopic expression of cyclin B1 in oocytes . (bvsalud.org)
  • Most Oocytes incubated with SOV blocked at germinal vesicles (GV) stage or M I to anaphase transition due to dynein dysfunction and ectopic transcription level of cyclin B1 . (bvsalud.org)
  • abstract = "The aim of this study was to examine the relationship between the developmental competence of oocytes and their total RNA and protein contents, and the level of Cyclin B1 transcription. (uab.cat)
  • 135 μm), separated into sub-groups of 10 oocytes per treatment-replicate and stored in liquid nitrogen until total RNA content analysis by spectophotometry, total protein content analysis by a colorimetric assay and Cyclin B1 transcription analysis by RT-PCR. (uab.cat)
  • This study showed that the RNA content and the Cyclin B1 RNA expression of prepubertal goat oocytes, and their development to embryos varied between the different size categories of the oocytes. (uab.cat)
  • Wild-type p53 have been shown to suppress cyclin B1 expression. (wikipedia.org)
  • Previous work has shown that high cyclin B1 expression levels are found in variety of cancers such as breast, cervical, gastric, colorectal, head and neck squamous cell, non-small-cell lung cancer, colon, prostate, oral and esophageal. (wikipedia.org)
  • Nuclear-dominant expression of cyclin B1 leads to poorer prognosis due to its weak activity compared to cytoplasmic cyclin B1. (wikipedia.org)
  • Interestingly, the expression of cyclin B1 was inhibited to about 60% in U87.Mxi1.14 cells. (uniprot.org)
  • METHODS: To investigate the role of cyclin B1 in proliferation and differentiation of hECs in menstrual cycle, the expression of cyclin B1 throughout the menstrual cycle was evaluated in hECs. (biomedsearch.com)
  • Cellular proliferation was evaluated with MTT test, cell cycle with propidium iodide (PI) staining and flow cytometry, apoptosis with FITC-Annexin V and the expression of cyclin B1 with Western blotting. (biomedsearch.com)
  • We examined expression and prognostic value of cyclin B1 and cyclin A, key regulators of the G2/M checkpoint of the cell cycle, in NSCLC and bronchial precursor lesions. (garvan.org.au)
  • Methods and results: Immunohistochemical expression of cyclin B1 and A was examined in 90 cases of stage I-II primary NSCLC and bronchial precursor lesions using tissue microarrays. (garvan.org.au)
  • Increased cyclin B1 and A expression was found in 40.9% and 58.9% of NSCLC cases respectively and was significantly higher in primary NSCLC, lymph node metastases and some bronchial precursor lesions compared to normal bronchial epithelium. (garvan.org.au)
  • Increased expression of cyclin A and cyclin B1 correlated with tumour type, poorly differentiated tumours and male gender. (garvan.org.au)
  • A significant association was found between increased cyclin B1 expression and reduced survival using Kaplan-Meier survival analysis. (garvan.org.au)
  • Cyclin A expression was not associated with survival. (garvan.org.au)
  • Expression of cyclin B1 and cyclin dependent kinase inhibitor p21 in lymphocytes in patients with systemic lupus erythematosus. (jrheum.org)
  • We measured the expression of cyclin B1 and CDK inhibitor p21 in peripheral blood lymphocytes (PBL) from patients with SLE and controls. (jrheum.org)
  • Bivariate distribution of cyclin B1 or p21 expression versus cellular DNA content was assessed by flow cytometry. (jrheum.org)
  • Expression of cyclin B1 was similar in all groups. (jrheum.org)
  • Huanglian represents a class of agents that can inhibit tumor cell growth by directly suppressing the expression of a cyclin subunit that is critical for cell cycle progression. (aspetjournals.org)
  • Potential diagnostic biomarkers: differential expression of LMP2/β1i and cyclin B1 in human uterine leiomyosarcoma. (semanticscholar.org)
  • article{Hayashi2014PotentialDB, title={Potential diagnostic biomarkers: differential expression of LMP2/β1i and cyclin B1 in human uterine leiomyosarcoma. (semanticscholar.org)
  • Mechanistically, we revealed that CREPT/RPRD1B interacted with Aurora B to regulate the expression of Cyclin B1 in gastric cancer cells. (lyceesdefumel.com)
  • Antisense inhibition of cyclin B1 accumulation decreases apoptosis, and ectopic cyclin B1 expression is sufficient to induce apoptosis. (ashpublications.org)
  • The expression of cyclin B1 is cell‐cycle regulated, peaking at the G 2 /M transition. (embopress.org)
  • Prolactin represses the expression of Menin, which is an endocrine tumor suppressor, through the upregulation of cyclin-dependent kinase inhibitors, and enhances the expression of FoxM1, which plays a crucial role in islet expansion 5,6. (docplayer.net)
  • Expression of human cyclins through the cell cycle . (wikipedia.org)
  • [6] Expression of cyclins detected immunocytochemically in individual cells in relation to cellular DNA content (cell cycle phase), [7] or in relation to initiation and termination of DNA replication during S-phase, can be measured by flow cytometry . (wikipedia.org)
  • In at least 25% of tumor cases high expression of CDK1Tyr15, pCDK1Thr161, Cyclin B1 (total) and pCyclin B1Ser126 was observed, compared to the low levels in normal vulvar squamous epithelium. (cancerrealitycheck.com)
  • In conclusion, cyclin A, cyclin D1, p21 and p27 expression can be a valuable marker of poor prognosis and tumor aggressiveness in OSCC. (omicsonline.org)
  • OBJECTIVE: The roles of cyclins and cyclin dependent kinase (CDK) inhibitors of lymphocytes in the pathogenesis of systemic lupus erythematosus (SLE) are unclear. (jrheum.org)
  • Dysregulation of the cell cycle characterizes many cancer subtypes, providing a rationale for developing cyclin-dependent kinase (CDK) inhibitors. (rcsb.org)
  • We examined positive regulators of the cell cycle (cyclin A, cyclin B1, and cyclin D1), cell cycle inhibitors (p16, p21, p27, p53, and Rb), and the proliferation markers Ki-67 and topoisomerase IIA by immunohistochemistry in a tissue microarray, comprising 136 cases of OSCC. (omicsonline.org)
  • The human cytomegalovirus (HCMV)-encoded protein kinase, pUL97, is considered a cyclin-dependent kinase (CDK) ortholog, due to shared structural and functional characteristics. (mdpi.com)
  • There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the cell cycle machinery. (cam.ac.uk)
  • CKIs, which inhibit certain cyclin/CDK complexes, also rise and decline at specific times during the cell cycle [ 4 ]. (omicsonline.org)
  • Different cyclin-CDK complexes regulate various cell cycle transitions. (omicsonline.org)
  • We reintroduced mutant proteins into this genetically null background to determine why Cyclin B1 is constantly exported from the nucleus. (cam.ac.uk)
  • Furthermore we ready a cyclin B1 mutant missing the complete CRS area (proteins 88-154) (cyclin B1ΔCRS). (biomarketx.net)
  • Thus the web nuclear deposition of cyclin B1 inside our program like this … The focus of Went Q69L found in these tests (2 μM) provides been proven to inhibit import of many proteins in various other laboratories (36-38). (biomarketx.net)
  • Cyclin is a family of proteins that control the progression of cells through the cell cycle by activating cyclin-dependent kinase (CDK) enzymes . (wikipedia.org)
  • This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. (novusbio.com)
  • Destruction of Cyclin B1 is required for cell cycle progression. (thermofisher.com)
  • Cyclin B1 is a key regulatory protein controlling cell cycle progression in vertebrates. (nih.gov)
  • BACKGROUND It has been reported that p53 regulates the G2-M checkpoint transition through cyclin B1, and it has been suggested that p53 plays an important role in the development and progression of various malignancies. (semanticscholar.org)
  • In conclusion, CDK1Tyr15, pCDK1Thr161, Cyclin B1 (total) and pCyclin B1Ser126 may be involved in progression of vulvar squamous cell carcinoma. (cancerrealitycheck.com)
  • Kaposi sarcoma herpesvirus ( KSHV ) encodes a D-type cyclin (ORF72) that binds CDK6 and is likely to contribute to KSHV-related cancers [9] . (wikipedia.org)
  • Here we report hitherto unknown mechanism by which cyclin G1 increases radiation sensitivity by regulating the level of cyclin B1. (elsevier.com)
  • These high levels of cyclin B1 can also be associated to the extent of tumor invasion and aggressiveness therefore concentration of cyclin B1 can be used to determine the prognosis of cancer patients. (wikipedia.org)
  • While the exact mechanism that explains how cyclin B1 becomes overexpressed is not very well understood, previous work has shown that down regulation of cyclin B1 can lead to tumor regression. (wikipedia.org)
  • Preferential autoimmune response in prostate cancer to cyclin B1 in a panel of tumor-associated antigens. (nih.gov)
  • In human cells, an inhibition of cyclin B1 transcription by the p53 tumor suppressor prevents G2/M transition. (ashpublications.org)
  • Elevated levels of CDK1Tyr15, pCDK1Thr161, Cyclin B1 (total) and pCyclin B1Ser126 were correlated with advanced tumor Tideglusib pontent inhibitor behaviors and aggressive features. (cancerrealitycheck.com)
  • Furthermore, DNA replication arrest down-regulates cyclin B1 promoter activity in non-transformed cells, but not in tumor cells in a Chk1- and p38-independent way. (aacrjournals.org)
  • To identify the mechanism by which p53 regulates G 2 , we have derived a human ovarian cell that undergoes p53-dependent G 2 arrest at 32°C. We have found that p53 prevents G 2 /M transition by decreasing intracellular levels of cyclin B1 protein and attenuating the activity of the cyclin B1 promoter. (pnas.org)
  • This inhibition occurs at the transcriptional level and depends, at least in part, on the E-box present on the cyclin B1 promoter. (uniprot.org)
  • Investigation of the mechanism by which methylation decreased Sp1 activity found that methylation of Sp1 increased the recruitment of Su(var) 3-9 homologue 1(Suv39H1) and histone deacetylase 1 (HDAC1) to the cyclin B1 promoter, resulting in deacetylation and methylation of histone H3 and subsequent downregulation of cyclin B1. (elsevier.com)
  • To evaluate the effect of dynein inhibitor on mouse oocyte in vitro maturation and its cyclin B1 transcription level. (bvsalud.org)
  • The zebrafish cyclin B1 3'UTR was quantitatively less effective at stimulating polyadenylation and translation compared to the Xenopus cyclin B1 3'UTR during both zebrafish and Xenopus oocyte maturation. (biomedcentral.com)
  • Ultimately, serum from cancer patients and healthy donors was analyzed for anti-cyclin B1 antibodies. (nih.gov)
  • Invitrogen Anti-Cyclin B1 Monoclonal (GNS1), Catalog # MA5-14319. (lyceesdefumel.com)
  • Anti-Cyclin Antibodies are ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research. (rockland-inc.com)
  • Inhibition of cyclin dependent kinase (cdk) activity is emerging as an attractive target for cancer chemotherapy. (aspetjournals.org)
  • We also didn't detect inhibition of cyclin import in the current presence of 8 μM Went Q69L (data not really proven). (biomarketx.net)
  • The role of cyclin B1 is to transition the cell from G2 to M phase but becomes unregulated in cancer cells where overexpression of cyclin B1 can lead to uncontrolled cell growth by binding to its partner Cdks. (wikipedia.org)
  • HN30 cells are from head and neck cancer tumors that over express cyclin B1 and D1. (antibodies-online.com)
  • This study shows that seliciclib is a high-affinity substrate of ATP-binding cassette B1 (ABCB1) because it activates the ATPase activity of the transporter with an EC 50 of 4.2 μM and shows vectorial transport in MDCKII-MDR1 cells, yielding an efflux ratio of 8. (aspetjournals.org)
  • In support of this hypothesis, we found that treatment of cells with leptomycin B, which disrupted Cyclin B1-CRM1 interactions, led to a marked nuclear accumulation of Cyclin B1. (duke.edu)
  • In conclusion, a high-affinity cyclin B1-derived HLA-A2-restricted CTL epitope was identified, which was presented on the cell surface of cancer cells, and elicited spontaneous T-cell responses in cancer patients and healthy donors. (nih.gov)
  • Fig.3 (excerpt): Early embryos in which some cells lack Cyclin B1 and are not dividing (on the left). (cam.ac.uk)
  • We find that abundance of the cyclin B1 protein rapidly increases in several mouse and human hematopoietic cells (Ramos, DP16, HL60, thymocytes) undergoing γ-radiation-induced apoptosis. (ashpublications.org)
  • We have found that cyclin B1 protein levels rapidly increase during γ-radiation-induced apoptosis in several mouse and human hematopoietic cells (Ramos, DP16, HL60) as well as in primary thymocytes. (ashpublications.org)
  • Each type of cyclin B1 was purified from lysates of baculovirus-infected insect cells and prebound to purified Cdc2K? (biomarketx.net)
  • CONCLUSION: TIS21 negatively regulated hepatocarcinogenesis in part by disruption of the FoxM1-cyclin B1 regulatory loop, thereby inhibiting proliferation of transformed cells developed in mouse and human livers. (ajou.ac.kr)
  • Cyclins can be divided into four classes based on their behavior in the cell cycle of vertebrate somatic cells and yeast cells: G1 cyclins, G1/S cyclins, S cyclins, and M cyclins. (wikipedia.org)
  • Irradiation of human lung cells with cyclin G1 overexpression resulted in increased cell death and γ-H2AX foci suggesting that cyclin G1 rendered the cells more susceptible to DNA damage. (elsevier.com)
  • Chuang, JY , Chang, WC & Hung, JJ 2011, ' Hydrogen peroxide induces Sp1 methylation and thereby suppresses cyclin B1 via recruitment of Suv39H1 and HDAC1 in cancer cells ', Free Radical Biology and Medicine , vol. 51, no. 12, pp. 2309-2318. (elsevier.com)
  • Natural killer T cells play an important role in liver regeneration, which is associated with cyclin B1 and interleukin-6. (biomedcentral.com)
  • This localization requires the cyclin box region of the protein. (nih.gov)
  • Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1. (duke.edu)
  • We show here that the previously characterized cytoplasmic retention sequence of Cyclin B1, responsible for its interphase cytoplasmic localization, is actually an autonomous nuclear export sequence, capable of directing nuclear export of a heterologous protein, and able to bind specifically to the recently identified export mediator, CRM1. (duke.edu)
  • We propose that the observed cytoplasmic localization of Cyclin B1 during interphase reflects the equilibrium between ongoing nuclear import and rapid CRM1-mediated export. (duke.edu)
  • The cyclin B cytoplasmic retention sequence (CRS), which is responsible for its interphase cytoplasmic localization, functions as a nuclear export sequence (Yang et al. (reactome.org)
  • Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G 1 /S transition. (rockland-inc.com)
  • Cyclin B1 can reside in the nucleus or the cytoplasm which can have an effect on the malignant potential of cyclin B1 when overexpressed in each location. (wikipedia.org)
  • The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. (fishersci.com)
  • So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. (fishersci.com)
  • Cyclin 131, the regulatory component of M phase-promoting factor (MPF), is degraded during the metaphase-anaphase transition in an anaphase-promoting complex/cyclosome (APC/C)-dependent process [1]. (ncl.ac.uk)
  • Cyclin D interacts with CDK4/6 and is necessary for the G1-S transition. (omicsonline.org)
  • Among its related pathways are ATM Pathway and Cyclin A/B1 associated events during G2/M transition . (genecards.org)
  • Recombinant protein encompassing a sequence within the center region of human Cyclin B1. (genetex.com)
  • AJOU Open Repository: TIS21 negatively regulates hepatocarcinogenesis by disruption of cyclin B1-Forkhead box M1 regulation loop. (ajou.ac.kr)
  • abstract = "Although cyclin G1 has been implicated in certain p53-related biological phenomena, other aspects of its function remain unclear. (elsevier.com)
  • During interphase, cyclin B1 shuttles continuously in and out of the nucleus. (reactome.org)
  • Cyclins themselves have no enzymatic activity but have binding sites for some substrates and target the Cdks to specific subcellular locations. (wikipedia.org)
  • 2013 ). MCC-bound APC/C is incapable of poly-ubiquitinating its metaphase substrates, securin and cyclin B1, at least in large part due to the actions of BUBR1, which occupies a substrate-recognition site on CDC20 and likely has additional inhibitory interactions with the APC/C (Lara-Gonzalez et al. (springer.com)
  • Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). (antibodies-online.com)
  • Involvement of cyclin B1 in progesterone-mediated cell growth inhibition, G2/M cell cycle arrest, and apoptosis in human endometrial cell. (biomedsearch.com)
  • Full length Clone DNA of Human cyclin B1 with N terminal Myc tag. (sinobiological.com)
  • Graf L, Webel R, Wagner S, Hamilton ST, Rawlinson WD, Sticht H, Marschall M. The Cyclin-Dependent Kinase Ortholog pUL97 of Human Cytomegalovirus Interacts with Cyclins. (mdpi.com)
  • Cyclin C was originally identified by a genetic screen for human and Drosophila cDNAs that complement a triple knock-out of the CLN genes in Saccharomyces cerevisiae. (novusbio.com)
  • Human cdk8-cyclin C might be functionally associated with the mammalian transcription apparatus, perhaps involved in relaying growth-regulatory signals. (novusbio.com)
  • Overexpression of cyclin D1 correlated with the presence of lymph node metastasis. (omicsonline.org)
  • The primary mechanism of CDK activation is binding to corresponding cyclins, including cyclin T1, which is the usual regulatory cofactor of CDK9. (mdpi.com)
  • G1 cyclins do not behave like the other cyclins, in that the concentrations increase gradually (with no oscillation), throughout the cell cycle based on cell growth and the external growth-regulatory signals. (wikipedia.org)
  • The objective of the current study was to clarify the role of the cell cycle regulators, cyclin B1 and p53, in patients with esophageal squamous cell carcinoma (ESCC). (semanticscholar.org)
  • CONCLUSION: Our findings suggest that cyclin B1 is a critical factor in proliferation and differentiation of hECs. (biomedsearch.com)
  • Progesterone may inhibit cell proliferation, mediate G2/M cell cycle arrest and induce apoptosis in hECs via down-regulating Cyclin B1. (biomedsearch.com)
  • Prognostic value of cyclin B1 in patients with esophageal squamous cell carcinoma. (semanticscholar.org)
  • Fig.55 and and and and and and (39) recently found that cyclin B1 interacts directly with the N-terminal half of importin β at a site distinct from the C-terminal area that interacts with importin α. (biomarketx.net)
  • We conclude that cyclin B1 import is normally mediated by a unique importin β-reliant system that will not need Went. (biomarketx.net)
  • In addition immunodepletion of the majority of importin β from crude cytosol led to a significant VX-689 decrease in the nuclear accumulation of cyclin B1ΔCRS-Cdc2K? (biomarketx.net)
  • In ACC patients, the overexpression of cyclin B1, TfR1, and fibronectin and combinations thereof were associated with poor prognosis. (e-enm.org)
  • Overexpression of cyclin G1 was observable in lung carcinoma tissues. (elsevier.com)
  • Overexpression of cyclin A, cyclin D1, topoisomerase IIA, and Rb and downregulation of p21 and p27 were associated with reduced overall survival in OSCC patients. (omicsonline.org)
  • These findings support that the Tuberin-CyclinB1 interaction is an important cellular checkpoint that protects cell integrity. (uwindsor.ca)
  • Cyclin B1 is displaced from individual kinetochores to the spindle poles by microtubule attachment to the kinetochores, and this displacement is dependent on the dynein/dynactin complex. (nih.gov)
  • The cyclins also promote other activities to progress the cell cycle, such as centrosome duplication in vertebrates or spindle pole body in yeast. (wikipedia.org)
  • To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, cyclin B1 (V152): sc-53236 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors. (scbt.com)
  • Therefore, our data suggest that cyclin G1 enhanced radiation sensitivity by overriding radiation-induced G2 arrest through transcriptional upregulation of cyclin B1. (elsevier.com)
  • Seliciclib, a cyclin-dependent kinase inhibitor, is a promising candidate to treat a variety of cancers. (aspetjournals.org)
  • Fig.44and and and … Nuclear Import of Cyclin B1 Can be an Energy-Dependent Procedure THAT WILL REQUIRE Importin β. (biomarketx.net)
  • These outcomes indicate that cyclin B1 import takes place by a dynamic saturable and cytosol-dependent procedure. (biomarketx.net)
  • In this report, we present data suggesting that cyclin B1 protein abundance regulates the apoptosis caused by γ-radiation. (ashpublications.org)
  • We show that Cyclin B1 −/− embryos arrest in G2 phase after just two divisions. (cam.ac.uk)
  • Autoantibodies to cyclin B1 and fourteen other TAAs were detected by enzyme-linked immunosorbent assay (ELISA) and Western blotting in 464 sera from patients with PCa, benign prostatic hyperplasia (BPH), and other controls. (nih.gov)