Cyclic AMP Response Element-Binding Protein: A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.Cyclic AMP Response Element-Binding Protein A: A basic leucine zipper transcription factor that is highly homologous to ACTIVATING TRANSCRIPTION FACTOR 2. It binds the consensus site TGACGTCA of the cyclic AMP response element in partnership with either PROTO-ONCOGENE PROTEINS C-JUN or activating transcription factor 2.CREB-Binding Protein: A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Access to Information: Individual's rights to obtain and use information collected or generated by others.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Suprachiasmatic Nucleus: An ovoid densely packed collection of small cells of the anterior hypothalamus lying close to the midline in a shallow impression of the OPTIC CHIASM.Period Circadian Proteins: Circadian rhythm signaling proteins that influence circadian clock by interacting with other circadian regulatory proteins and transporting them into the CELL NUCLEUS.Circadian Rhythm: The regular recurrence, in cycles of about 24 hours, of biological processes or activities, such as sensitivity to drugs and stimuli, hormone secretion, sleeping, and feeding.Biological Clocks: The physiological mechanisms that govern the rhythmic occurrence of certain biochemical, physiological, and behavioral phenomena.Ananas: A plant genus of the family BROMELIACEAE known for the edible fruit that is the source of BROMELAINS.Circadian Clocks: Biological mechanism that controls CIRCADIAN RHYTHM. Circadian clocks exist in the simplest form in cyanobacteria and as more complex systems in fungi, plants, and animals. In humans the system includes photoresponsive RETINAL GANGLION CELLS and the SUPRACHIASMATIC NUCLEUS that acts as the central oscillator.Pericytes: Unique slender cells with multiple processes extending along the capillary vessel axis and encircling the vascular wall, also called mural cells. Pericytes are imbedded in the BASEMENT MEMBRANE shared with the ENDOTHELIAL CELLS of the vessel. Pericytes are important in maintaining vessel integrity, angiogenesis, and vascular remodeling.Coronary Artery Disease: Pathological processes of CORONARY ARTERIES that may derive from a congenital abnormality, atherosclerotic, or non-atherosclerotic cause.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Neovascularization, Physiologic: The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.Endothelial Cells: Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.Stem Cell Transplantation: The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.Advisory Committees: Groups set up to advise governmental bodies, societies, or other institutions on policy. (Bioethics Thesaurus)Muscle Rigidity: Continuous involuntary sustained muscle contraction which is often a manifestation of BASAL GANGLIA DISEASES. When an affected muscle is passively stretched, the degree of resistance remains constant regardless of the rate at which the muscle is stretched. This feature helps to distinguish rigidity from MUSCLE SPASTICITY. (From Adams et al., Principles of Neurology, 6th ed, p73)Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Stereoisomerism: The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Mycobacterium tuberculosis: A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.Mycobacterium marinum: A moderate-growing, photochromogenic species found in aquariums, diseased fish, and swimming pools. It is the cause of cutaneous lesions and granulomas (swimming pool granuloma) in humans. (Dorland, 28th ed)Tuberculosis: Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.Mycobacterium: A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.Interferon-gamma: The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Gangliosides: A subclass of ACIDIC GLYCOSPHINGOLIPIDS. They contain one or more sialic acid (N-ACETYLNEURAMINIC ACID) residues. Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1997)Down-Regulation: A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.G(M1) Ganglioside: A specific monosialoganglioside that accumulates abnormally within the nervous system due to a deficiency of GM1-b-galactosidase, resulting in GM1 gangliosidosis.Leukemia, Lymphocytic, Chronic, B-Cell: A chronic leukemia characterized by abnormal B-lymphocytes and often generalized lymphadenopathy. In patients presenting predominately with blood and bone marrow involvement it is called chronic lymphocytic leukemia (CLL); in those predominately with enlarged lymph nodes it is called small lymphocytic lymphoma. These terms represent spectrums of the same disease.Genes, bcl-2: The B-cell leukemia/lymphoma-2 genes, responsible for blocking apoptosis in normal cells, and associated with follicular lymphoma when overexpressed. Overexpression results from the t(14;18) translocation. The human c-bcl-2 gene is located at 18q24 on the long arm of chromosome 18.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.bcl-2-Associated X Protein: A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein.Hydroxyeicosatetraenoic Acids: Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.Muscle, Smooth, Vascular: The nonstriated involuntary muscle tissue of blood vessels.Myocytes, Smooth Muscle: Non-striated, elongated, spindle-shaped cells found lining the digestive tract, uterus, and blood vessels. They are derived from specialized myoblasts (MYOBLASTS, SMOOTH MUSCLE).Lipoxygenase: An enzyme of the oxidoreductase class primarily found in PLANTS. It catalyzes reactions between linoleate and other fatty acids and oxygen to form hydroperoxy-fatty acid derivatives.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.12-Hydroxy-5,8,10,14-eicosatetraenoic Acid: A lipoxygenase metabolite of ARACHIDONIC ACID. It is a highly selective ligand used to label mu-opioid receptors in both membranes and tissue sections. The 12-S-HETE analog has been reported to augment tumor cell metastatic potential through activation of protein kinase C. (J Pharmacol Exp Ther 1995; 274(3):1545-51; J Natl Cancer Inst 1994; 86(15):1145-51)Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Tumor Microenvironment: The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.Genes, ras: Family of retrovirus-associated DNA sequences (ras) originally isolated from Harvey (H-ras, Ha-ras, rasH) and Kirsten (K-ras, Ki-ras, rasK) murine sarcoma viruses. Ras genes are widely conserved among animal species and sequences corresponding to both H-ras and K-ras genes have been detected in human, avian, murine, and non-vertebrate genomes. The closely related N-ras gene has been detected in human neuroblastoma and sarcoma cell lines. All genes of the family have a similar exon-intron structure and each encodes a p21 protein.Cell Line, Tumor: A cell line derived from cultured tumor cells.Disease Progression: The worsening of a disease over time. This concept is most often used for chronic and incurable diseases where the stage of the disease is an important determinant of therapy and prognosis.Neoplasm Metastasis: The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.

ATF-2-binding regulatory element is responsible for the Ly49A expression in murine T lymphoid line, EL-4. (1/3445)

To understand the mechanism of Ly49A-expression and its significance in T-cell differentiation, we analyzed the 5'-flanking region of the Ly49A gene in a search for the Ly49A-regulatory element. Since very few known regulatory elements have been found in this region, presumably a novel regulatory sequence(s) could exist. Accordingly, we defined the 13-bp regulatory element, 5'-ATGACGAGGAGGA-3', restricted to Ly49A-expression in EL-4 cells in comparison with two other representative cell lines tested. This element, designated as EL13, proved to be previously undiscovered by homology search and is highly homologous with several virus DNAs. Using EL13 as a probe we have cloned a cDNA encoding a binding protein to EL13. Its deduced nucleotide sequence revealed that EL13-binding protein is almost identical with rat ATF-2. Although ATF-2 is known to bind to cyclic AMP responsive element (CRE), EL13 shares five out of eight nucleotides with this consensus sequence. Our results suggested that ATF-2 may play an important role via binding to EL13 for the expression of Ly49A. These data will provide useful information for understanding T-cell and NK-cell differentiation in murine immune system.  (+info)

A critical role for cAMP response element-binding protein (CREB) as a Co-activator in sterol-regulated transcription of 3-hydroxy-3-methylglutaryl coenzyme A synthase promoter. (2/3445)

3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, a key regulatory enzyme in the pathway for endogenous cholesterol synthesis, is a target for negative feedback regulation by cholesterol. When cellular sterol levels are low, the sterol regulatory element-binding proteins (SREBPs) are released from the endoplasmic reticulum membrane, allowing them to translocate to the nucleus and activate SREBP target genes. However, in all SREBP-regulated promoters studied to date, additional co-regulatory transcription factors are required for sterol-regulated activation of transcription. We have previously shown that, in addition to SREBPs, NF-Y/CBF is required for sterol-regulated transcription of HMG-CoA synthase. This heterotrimeric transcription factor has recently been shown to function as a co-regulator in several other SREBP-regulated promoters, as well. In addition to cis-acting sites for both SREBP and NF-Y/CBF, the sterol regulatory region of the synthase promoter also contains a consensus cAMP response element (CRE), an element that binds members of the CREB/ATF family of transcription factors. Here, we show that this consensus CRE is essential for sterol-regulated transcription of the synthase promoter. Using in vitro binding assays, we also demonstrate that CREB binds to this CRE, and mutations within the CRE that result in a loss of CREB binding also result in a loss of sterol-regulated transcription. We further show that efficient activation of the synthase promoter in Drosophila SL2 cells requires the simultaneous expression of all three factors: SREBPs, NF-Y/CBF, and CREB. To date this is the first promoter shown to require CREB for efficient sterol-regulated transcription, and to require two different co-regulatory factors in addition to SREBPs for maximal activation.  (+info)

CCAAT/enhancer-binding protein beta is an accessory factor for the glucocorticoid response from the cAMP response element in the rat phosphoenolpyruvate carboxykinase gene promoter. (3/3445)

The cyclic AMP response element (CRE) of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene promoter is required for a complete glucocorticoid response. Proteins known to bind the PEPCK CRE include the CRE-binding protein (CREB) and members of the CCAAT/enhancer-binding protein (C/EBP) family. We took two different approaches to determine which of these proteins provides the accessory factor activity for the glucocorticoid response from the PEPCK CRE. The first strategy involved replacing the CRE of the PEPCK promoter/chloramphenicol acetyltransferase reporter plasmid (pPL32) with a consensus C/EBP-binding sequence. This construct, termed pDeltaCREC/EBP, binds C/EBPalpha and beta but not CREB, yet it confers a nearly complete glucocorticoid response when transiently transfected into H4IIE rat hepatoma cells. These results suggest that one of the C/EBP family members may be the accessory factor. The second strategy involved co-transfecting H4IIE cells with a pPL32 mutant, in which the CRE was replaced with a GAL4-binding sequence (pDeltaCREGAL4), and various GAL4 DNA-binding domain (DBD) fusion protein expression vectors. Although chimeric proteins consisting of the GAL4 DBD fused to either CREB or C/EBPalpha are able to confer an increase in basal transcription, they do not facilitate the glucocorticoid response. In contrast, a fusion protein consisting of the GAL4 DBD and amino acids 1-118 of C/EBPbeta provides a significant glucocorticoid response. Additional GAL4 fusion studies were done to map the minimal domain of C/EBPbeta needed for accessory factor activity to the glucocorticoid response. Chimeric proteins containing amino acid regions 1-84, 52-118, or 85-118 of C/EBPbeta fused to the GAL4 DBD do not mediate a glucocorticoid response. We conclude that the amino terminus of C/EBPbeta contains a multicomponent domain necessary to confer accessory factor activity to the glucocorticoid response from the CRE of the PEPCK gene promoter.  (+info)

CRE-mediated gene transcription in neocortical neuronal plasticity during the developmental critical period. (4/3445)

Neuronal activity-dependent processes are believed to mediate the formation of synaptic connections during neocortical development, but the underlying intracellular mechanisms are not known. In the visual system, altering the pattern of visually driven neuronal activity by monocular deprivation induces cortical synaptic rearrangement during a postnatal developmental window, the critical period. Here, using transgenic mice carrying a CRE-lacZ reporter, we demonstrate that a calcium- and cAMP-regulated signaling pathway is activated following monocular deprivation. We find that monocular deprivation leads to an induction of CRE-mediated lacZ expression in the visual cortex preceding the onset of physiologic plasticity, and this induction is dramatically downregulated following the end of the critical period. These results suggest that CRE-dependent coordinate regulation of a network of genes may control physiologic plasticity during postnatal neocortical development.  (+info)

Platelet-derived growth factor induces interleukin-6 transcription in osteoblasts through the activator protein-1 complex and activating transcription factor-2. (5/3445)

Platelet-derived growth factor (PDGF) BB, a mitogen that stimulates bone resorption, increases the expression of interleukin-6 (IL-6), a cytokine that induces osteoclast recruitment. The mechanisms involved in IL-6 induction by PDGF BB are poorly understood. We examined the effect of PDGF BB on IL-6 expression in cultures of osteoblasts from fetal rat calvariae (Ob cells). PDGF BB increased IL-6 mRNA and heterogeneous nuclear RNA levels, the rate of transcription, and the activity of base pairs (bp) -2906 to +20 IL-6 promoter fragments transiently transfected into Ob cells. Deletion analysis revealed two responsive regions, one containing an activator protein-1 (AP-1) site located between bp -276 and -257, and a second, less well defined, downstream of -257. Targeted mutations of a cyclic AMP-responsive element (CRE), and nuclear factor-IL-6 and nuclear factor-kappaB binding sites in a bp -257 to +20 IL-6 construct that was transfected into Ob cells, revealed that the CRE also contributed to IL-6 promoter induction by PDGF BB. Electrophoretic mobility shift assay revealed AP-1 and CRE nuclear protein complexes that were enhanced by PDGF BB. Supershift assays revealed binding of Jun and Fos to AP-1 and CRE sequences and binding of activating transcription factor-2 to CRE. In conclusion, PDGF BB induces IL-6 transcription in osteoblasts by regulating nuclear proteins of the AP-1 complex and activating transcription factor-2.  (+info)

pp60(v-src) induction of cyclin D1 requires collaborative interactions between the extracellular signal-regulated kinase, p38, and Jun kinase pathways. A role for cAMP response element-binding protein and activating transcription factor-2 in pp60(v-src) signaling in breast cancer cells. (6/3445)

The cyclin D1 gene is overexpressed in breast tumors and encodes a regulatory subunit of cyclin-dependent kinases that phosphorylate the retinoblastoma protein. pp60(c-src) activity is frequently increased in breast tumors; however, the mechanisms governing pp60(c-src) regulation of the cell cycle in breast epithelium are poorly understood. In these studies, pp60(v-src) induced cyclin D1 protein levels and promoter activity (48-fold) in MCF7 cells. Cyclin D1-associated kinase activity and protein levels were increased in mammary tumors from murine mammary tumor virus-pp60(c-src527F) transgenic mice. Optimal induction of cyclin D1 by pp60(v-src) involved the extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase members of the mitogen-activated protein kinase family. Cyclin D1 promoter activation by pp60(v-src) involved a cAMP response element-binding protein (CREB)/activating transcription factor 2 (ATF-2) binding site. Dominant negative mutants of CREB and ATF-2 but not c-Jun inhibited pp60(v-src) induction of cyclin D1. pp60(v-src) induction of CREB was blocked by the p38 inhibitor SB203580 or by mutation of CREB at Ser133. pp60(v-src) induction of ATF-2 was abolished by the c-Jun N-terminal kinase inhibitor JNK-interacting protein-1 or by mutation of ATF-2 at Thr69 and Thr71. CREB and ATF-2, which bind to a common pp60(v-src) response element, are transcriptionally activated by distinct mitogen-activated protein kinases. Induction of cyclin D1 activity by pp60(v-src) may contribute to breast tumorigenesis through phosphorylation and inactivation of the retinoblastoma protein.  (+info)

CREB regulates MHC class II expression in a CIITA-dependent manner. (7/3445)

The X2 box of MHC class II promoters is homologous to TRE/CRE elements and is required for expression of MHC class II genes. The X2 box-specific DNA binding activity, X2BP, was purified to homogeneity, sequenced, and identified as CREB. Transient transactivation experiments showed that CREB can cooperate with CIITA to enhance activation of transcription from MHC class II promoters in a dose-dependent manner. Binding of CREB to the class II promoter in vivo was demonstrated by a chromatin immunoprecipitation assay. Additionally, ICER, a dominant inhibitor of CREB function, was found to repress class II expression. These results demonstrate that CREB binds to the X2 box in vivo and cooperates with CIITA to direct MHC class II expression.  (+info)

Interaction of Gli2 with CREB protein on DNA elements in the long terminal repeat of human T-cell leukemia virus type 1 is responsible for transcriptional activation by tax protein. (8/3445)

The long terminal repeat (LTR) of human T-cell leukemia virus type 1 (HTLV-1) has two distinct DNA elements, one copy of TRE2S and three copies of a 21-bp sequence that respond to the viral trans-activator protein, Tax. Either multiple copies of the 21-bp sequence or a combination of one copy each of TRE2S and 21-bp sequence is required for efficient trans activation by Tax. In the trans activation of multiple copies of 21-bp sequence, CREB/ATF protein plays an essential role in forming a complex with Tax. To understand the role of TRE2S in trans activation of one copy of 21-bp sequence, we examined protein binding to the DNA elements by DNA affinity precipitation assay including Gli2 protein binding to TRE2S and CREB protein binding to 21-bp sequence. Binding of CREB to a DNA probe containing both elements, TRE2S-21bp probe, was dependent on Gli2 protein under restricted conditions and was enhanced in a dose-dependent fashion by the binding of Gli2 protein to the same probe. Mutation in either element abolished the efficient binding of CREB. A glutathione S-transferase fusion protein of a fragment of Gli2 was able to bind to CREB. Therefore, Gli2-CREB interaction on the DNA probe is proposed to stabilize CREB binding to DNA. Tax can bind to CREB protein on the DNA; therefore, stabilization of DNA binding of CREB results in more recruitment of Tax onto DNA. Conversely, Tax increased the DNA binding of CREB, although it had almost no effect on the binding of Gli2. These results suggest that Gli2 binds to the DNA element and interacts with CREB, resulting in more recruitment of Tax, which in turn stabilizes DNA binding of CREB. Similar cooperation of the protein binding to TRE2S-21bp probe was also observed in nuclear extract of an HTLV-1-infected T-cell line. Consistent with the Gli2-CREB interaction on the DNA elements, Tax-mediated trans activation was dependent on the size of the spacer between TRE2S and 21-bp sequence. The effective sizes of the spacer suggest that TRE2S in the LTR would cooperate with the second and third copies of the 21-bp sequence and contribute to trans activation of the viral gene transcription.  (+info)

*CAMP responsive element modulator

"Cyclic AMP response element binding protein CREB and modulator protein CREM are products of distinct genes". Nucleic Acids ... Pongubala JM, Atchison ML (Apr 1995). "Activating transcription factor 1 and cyclic AMP response element modulator can modulate ... "The cyclic AMP response element modulator family regulates the insulin gene transcription by interacting with transcription ... Fujimoto T, Fujisawa J, Yoshida M (Feb 1994). "Novel isoforms of human cyclic AMP-responsive element modulator (hCREM) mRNA". ...

*RELA

Parry GC, Mackman N (Dec 1997). "Role of cyclic AMP response element-binding protein in cyclic AMP inhibition of NF-kappaB- ... "Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with ... Gerritsen ME, Williams AJ, Neish AS, Moore S, Shi Y, Collins T (Apr 1997). "CREB-binding protein/p300 are transcriptional ... Aarnisalo P, Palvimo JJ, Jänne OA (Mar 1998). "CREB-binding protein in androgen receptor-mediated signaling". Proceedings of ...

*Activating transcription factor 2

Kara CJ, Liou HC, Ivashkiv LB, Glimcher LH (April 1990). "A cDNA for a human cyclic AMP response element-binding protein which ... Kara CJ, Liou HC, Ivashkiv LB, Glimcher LH (1990). "A cDNA for a human cyclic AMP response element-binding protein which is ... "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain". EMBO J. 8 (7): 2023-8. ... "Assignment of the gene for cyclic AMP-response element binding protein 2 (CREB2) to human chromosome 2q24.1-q32". Genomics. 11 ...

*ICAM-1

"ICAM-1-coupled signaling pathways in astrocytes converge to cyclic AMP response element-binding protein phosphorylation and TNF ... The presence of heavy glycosylation and other structural characteristics of ICAM-1 lend the protein binding sites for numerous ... ICAM-1 possesses binding sites for a number of immune-associated ligands. Notably, ICAM-1 binds to macrophage adhesion ligand-1 ... and the protein's extracellular domain is composed of multiple loops created by disulfide bridges within the protein. The ...

*C-jun

Kara CJ, Liou HC, Ivashkiv LB, Glimcher LH (1990). "A cDNA for a human cyclic AMP response element-binding protein which is ... "Isolation and characterization of a novel member of the gene family encoding the cAMP response element-binding protein CRE-BP1 ... c-Jun is a protein that in humans is encoded by the JUN gene. c-Jun in combination with c-Fos, forms the AP-1 early response ... The cyclic change of the c-jun protein levels is significant in the proliferation and apoptosis of glandular epithelial cells. ...

*Kaang Bong-kiun

Lee JA, Kim H, Lee YS, Kaang BK (2003). "Overexpression and RNA interference of Ap-cyclic AMP-response element binding protein- ... "AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic ... transcription requires the protein kinase-A-mediated phosphorylation of the cAMP-response element-binding protein(CREB). ... he found that multiple pulses of serotonin stimulate gene expression that is mediated by the cAMP-response element(CRE). He ...

*Nerve growth factor

... act to phosphorylate the cyclic AMP response element binding protein (CREB) transcription factor. Phosphorylated CREB ... Survival and PCD mechanisms are mediated through adaptor protein binding to the death domain of the p75NTR cytoplasmic tail. ... This pathway begins with the Trk receptor complex-recruitment of a second adaptor protein called growth factor-receptor bound ... Protein targeting Nervous System VGF Nerve Growth Factor-inducible, a protein whose expression is induced by NGF Neurotrophin ...

*Epigenetics of depression

Cyclic-AMP Response Element Binding Protein (CREB), which is thought to be involved in GDNF regulation, associates with the ... CREB Binding Protein. Therefore, this antidepressant, by increasing acetylation, works to lessen the HPA response, and as a ... It is thought that this is happening due to an interaction between the response element of GR and the acetyltransferase, ... Furthermore, the methylation of the promoter region results in a decrease in recruitment of the CREB-Binding Protein (CBP), ...

*Barrel cortex

... calmodulin-dependent protein kinase II (CaMKII) or cyclic-AMP response element binding protein (CREB). In these mice, ... Stimulation of multiple whiskers may produce a response that is equal to the sum of the responses if each whisker was ... In deprived cortex, neuronal responses to spared whiskers are enhanced and responses to deprived whiskers are weakened. These ... with the weakening of deprived response preceding the strengthening of spared response, implying that they have different ...

*CREB

Drosophila Cyclic-AMP response element binding protein A - The Interactive Fly Drosophila Cyclic-AMP response element binding ... 170-6. ISBN 978-0-87893-697-7. Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. ... The protein also has a magnesium ion that facilitates binding to DNA. The cAMP response element (CRE) is the response element ... CREB (cAMP response element-binding protein) is a cellular transcription factor. It binds to certain DNA sequences called cAMP ...

*Activity-regulated cytoskeleton-associated protein

7 kb upstream that contains binding sites for cyclic AMP response element-binding protein (CREB), myocyte enhancer factor 2 ( ... a serum response element (SRE; see serum response factor) at ~1.5 kb upstream of the initiation site. a second SRE at ~6.5 kb; ... The common factor for these signaling molecules involves activation of cyclic-AMP and its downstream target protein kinase A ( ... Additionally, the protein has binding sites for endophilin 3 and dynamin 2 at amino acids 89-100 and 195-214, respectively. ...

*Molecular and epigenetic mechanisms of alcoholism

Chronic ethanol administration decreases phosphorylation of cyclic AMP response element-binding protein in granule cells of rat ... exposure alters the phosphorylation of cyclic AMP responsive element binding protein and cyclic AMP responsive element binding ... Pandey, S.C. (2004). The gene transcription factor cyclic AMP responsive element binding protein: role in positive and negative ... cyclic monophosphate response element binding protein activity in an age- and brain region-specific manner. Alcohol Clin Exp ...

*CREB-binding protein

Parry GC, Mackman N (December 1997). "Role of cyclic AMP response element-binding protein in cyclic AMP inhibition of NF-kappaB ... "cAMP-response-element-binding-protein-binding protein (CBP) and p300 are transcriptional co-activators of early growth response ... First isolated as a nuclear protein that binds to cAMP-response element-binding protein (CREB), this gene is now known to play ... Sp1 and cAMP-response-element-binding protein-binding protein (CBP/p300)". The Biochemical Journal. 339 (3): 751-8. doi:10.1042 ...

*CREB5

Cyclic AMP-responsive element-binding protein 5 is a protein that in humans is encoded by the CREB5 gene. The product of this ... cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The ... "Isolation and characterization of a novel member of the gene family encoding the cAMP response element-binding protein CRE-BP1 ... "Entrez Gene: CREB5 cAMP responsive element binding protein 5". Human CREB5 genome location and CREB5 gene details page in the ...

*PFKFB3

... and progesterone response element. Expression of the promoter is shown to be induce by phorbol esters and cyclic-AMP-dependent ... N-terminal structure that secures the binding of fructose-6-phosphate to the active site via interaction with the protein's '2- ... The promoter of PFKFB3 contains binding sites, called hypoxia response elements (HREs), that recruit the binding of hypoxia- ... The PFKFB3 promoter is predicted to contain multiple binding sites, including Sp-1 and AP-2 binding sites. It also contains ...

*CREB1

Pandey SC (Oct 2004). "The gene transcription factor cyclic AMP-responsive element binding protein: role in positive and ... is a protein that in humans is encoded by the CREB1 gene. This protein binds the cAMP response element, a DNA nucleotide ... "Synergism between calcium and cyclic GMP in cyclic AMP response element-dependent transcriptional regulation requires ... This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by ...

*Luteinizing hormone/choriogonadotropin receptor

DNA in the cell nucleus binds to phosphorylated proteins through the cyclic AMP response element (CRE), which results in the ... Cyclic AMP-dependent protein kinases (protein kinase A) are activated by the signal chain coming from the G protein (that was ... via adenylate cyclase and cyclic AMP (cAMP). These protein kinases are present as tetramers with two regulatory units and two ... The cyclic AMP-regulatory dimers are degraded by phosphodiesterase and release 5'AMP. ...

*Follicle-stimulating hormone receptor

DNA in the cell nucleus binds to phosphorylated proteins through the cyclic AMP response element (CRE), which results in the ... Cyclic AMP-dependent protein kinases (protein kinase A) are activated by the signal chain coming from the G protein (that was ... via adenylate cyclase and cyclic AMP (cAMP). These protein kinases are present as tetramers with two regulatory units and two ... This event leads to a transduction of the signal that activates the G protein that is bound to the receptor internally. With ...

*CREB/ATF bZIP transcription factor

"Cooperative interaction of Zhangfei and ATF4 in transactivation of the cyclic AMP response element". FEBS Lett. 580 (1): 58-62 ... Akhova O, Bainbridge M, Misra V (2005). "The neuronal host cell factor-binding protein Zhangfei inhibits herpes simplex virus ... CREB/ATF bZIP transcription factor is a protein that in humans is encoded by the CREBZF gene. GRCh38: Ensembl release 89: ... "Zhangfei is a potent and specific inhibitor of the host cell factor-binding transcription factor Luman". J. Biol. Chem. 280 (15 ...

*Neuronal memory allocation

... cyclic AMP responsive element-binding protein) in this process. Certain synapses on recruited neurons are more likely to ... The transcription factor cAMP response element-binding protein (CREB) is a well-studied mechanism of neuronal memory allocation ... Nguyen, P. V., & Woo, N. H. (2003). Regulation of hippocampal synaptic plasticity by cyclic AMP-dependent protein kinases. ... For example, the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) pathways appear to participate in neuronal ...

*TAF4

Inada A, Someya Y, Yamada Y, Ihara Y, Kubota A, Ban N, Watanabe R, Tsuda K, Seino Y (1999). "The cyclic AMP response element ... CBX5m TATA binding protein, and Transcription initiation protein SPT3 homolog. Yeast TFIID comprises the TATA binding protein ... and transactivation-defective mutants of human immunodeficiency virus type 1 Tat protein bind human TATA-binding protein in ... The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ...

*CEBPB

"Synergism between calcium and cyclic GMP in cyclic AMP response element-dependent transcriptional regulation requires ... CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene. The protein encoded by this ... In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I ... Hanlon M, Sealy L (May 1999). "Ras regulates the association of serum response factor and CCAAT/enhancer-binding protein beta ...

*Eric Kandel

One of the nuclear targets for PKA is the transcriptional control protein CREB (cAMP response element binding protein). In ... By 1972 they had evidence that the second messenger molecule cyclic AMP (cAMP) was produced in Aplysia ganglia under conditions ... By 1980, collaboration with Paul Greengard resulted in demonstration that cAMP-dependent protein kinase, also known as protein ... He also proposed a symposium on the response of Austria to National Socialism. Kandel has since accepted an honorary ...

*ATF5

... cAMP response-element binding protein (CREB) family. ATF5 transcripts and protein are expressed in a wide variety of tissues, ... Pati D, Meistrich ML, Plon SE (1999). "Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP- ... "Human Cdc34 and Rad6B ubiquitin-conjugating enzymes target repressors of cyclic AMP-induced transcription for proteolysis". Mol ... "The molecular biology and nomenclature of the activating transcription factor/cAMP responsive element binding family of ...

*Picornain 3C

Also, 3C and 2A are responsible for down-regulation of cyclic AMP responsive element binding protein (CREB), a cellular ... antiviral response. Cys24Ser (C24S) is a homolog of Hepatitis 3C proteinase, is responsible for inactivating Cys172 through ... binding protein, PABP. This disruption of this binding domain results in down regulation of the initiation of cap dependent ... Polioviruses' protease 3C is responsible for host cell transcription shut off by cleaving the TATA-binding protein and other ...

*Riboswitch

... "cyclic di-GMP" below). cyclic di-AMP riboswitches (also called ydaO/yuaA) bind the signaling molecule cyclic di-AMP. cyclic di- ... including protein-free binding assays, and metabolite-binding riboswitches were established as a new mechanism of gene ... The aptamer directly binds the small molecule, and the expression platform undergoes structural changes in response to the ... Cobalamin riboswitch (also B12-element), which binds either adenosylcobalamin (the coenzyme form of vitamin B12) or ...
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TY - JOUR. T1 - Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15. AU - Xie, Fuchun. AU - Fan, Qiuhua. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. PY - 2019/1/1. Y1 - 2019/1/1. N2 - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, we designed an ester prodrug of 666-15 through a long-range O,N-acyl transfer reaction for improved aqueous solubility. Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB.. AB - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, ...
ATM has been shown to have important roles in the transcriptional regulation of gene expression by phosphorylation of transcription factors, such as p53, nuclear factor κB, E2F, cyclic AMP-responsive element binding protein, and BRCA1, and in maintenance of the normal functions of IGF-IR and telomeres (3, 13, 14, 32). ATM-dependent gene expression has been systematically studied by comparing cell lines from normal individuals and AT patients, isogenic cell lines with restoration of ATM in AT cells or small interfering RNA knockdown of ATM in wild-type cells, and ATM+/+/ATM−/− mice (3, 13, 21-23). More than 300 genes have been reported to display ATM-dependent expression although few genes were commonly identified in two or more experiments. The microarray results presented here identified 160 genes or expressed sequence tags (∼1% of the total on the array) that were differentially expressed in normal and AT fibroblast lines under basal growth conditions, of which about half were ...
Lysosomal-associated protein transmembrane-4 beta (LAPTM4B) is a potential proto-oncogene, whose overexpression is involved in cancer occurrence and progression. Its transcript is up-regulated in various types of solid tumors including breast cancer. However, its transcriptional regulation mechanism is still unclear. To investigate the mechanism of transcriptional regulation of LAPTM4B in human breast cancer cells, a series of luciferase reporter constructs and construct with mutated binding site for cAMP responsive element binding protein-1 (CREB1) were generated by PCR amplification and transiently transfected into breast cancer cells to determine the transcriptional activities of different promoter regions. The + 10 similar to+ 292 promoter region was possessed the highest transcriptional activity. The ability of CREB1 to bind the LAPMT4B promoter was confirmed by electrophoretic mobility shift assay, super-shift and RNA interference experiments. Our study identified the core promoter region ...
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity).
TY - JOUR. T1 - Design, synthesis and biological evaluation of regioisomers of 666-15 as inhibitors of CREB-mediated gene transcription. AU - Xie, Fuchun. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. PY - 2016/11/3. Y1 - 2016/11/3. N2 - cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the pathogenesis and maintenance of various types of human cancers. Identification of small molecule inhibitors of CREB-mediated gene transcription has been pursued as a novel strategy for developing cancer therapeutics. We recently discovered a potent and cell-permeable CREB inhibitor called 666-15. 666-15 is a bisnaphthamide and has been shown to possess efficacious anti-breast cancer activity without toxicity in vivo. In this study, we designed and synthesized a series of analogs of 666-15 to probe the importance of regiochemistry in naphthalene ring B. Biological evaluations of these analogs demonstrated that the substitution pattern of the alkoxy and ...
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
TY - JOUR. T1 - Nuclear factor-kappaB and cAMP response element binding protein mediate opposite transcriptional effects on the Flk-1/KDR gene promoter.. AU - Illi, B.. AU - Puri, P.. AU - Morgante, L.. AU - Capogrossi, M. C.. AU - Gaetano, C.. PY - 2000/6/23. Y1 - 2000/6/23. N2 - -The vascular endothelial growth factor receptor Flk-1/KDR is highly expressed during development and almost disappears in adult tissues. Despite its biological relevance, little is known about the molecular mechanisms controlling its expression. In the present work, it is shown that cAMP response element binding protein (CREB) and nuclear factor-kappaB (NF-kappaB)-related antigens bind specific sequences in the Flk-1/KDR promoter. Functional studies demonstrate that cAMP represses whereas tumor necrosis factor-alpha, an activator of NF-kappaB, stimulates promoter activity. Histone acetyltransferases (HATs) P/CAF and CBP/p300 together with p65/RelA, the catalytic subunit of NF-kappaB, increase Flk-1/KDR promoter ...
The results reveal an essential survival pathway in malignant glioma, whereby activation of a RAS-mitogen-activated protein kinase or phosphoinositide-3-kinase signaling cascade leads to induction of the transcription factor cAMP response element-binding protein-3-like-2 (CREB3L2), which directly activates ATF5 expression. ATF5, in turn, promotes survival by stimulating transcription of myeloid cell leukemia sequence-1 (MCL1), an antiapoptotic B cell leukemia-2 family member. [Nat Med ...
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TransAM CREB and TransAM pCREB Kits are DNA-binding ELISAs that quanify the activated transcription factors using a method that is faster and more sensitive than gelshift, without radioactivity and gels.
Background: Lowered sensitivity to the effects of ethanol increases the risk of developing alcoholism. Inbred mouse strains have been useful for the study of the genetic basis of various drug addiction-related phenotypes. Inbred Long-Sleep (ILS) and Inbred Short-Sleep (ISS) mice differentially express a number of genes thought to be implicated in sensitivity to the effects of ethanol. Concomitantly, there is evidence for a mediating role of cAMP/PKA/CREB signalling in aspects of alcoholism modelled in animals. In this report, the extent to which CREB signalling impacts the differential expression of genes in ILS and ISS mouse cerebella is examined. Results: A training dataset for Machine Learning (ML) and Exploratory Data Analyses (EDA) was generated from promoter region sequences of a set of genes known to be targets of CREB transcription regulation and a set of genes whose transcription regulations are potentially CREB-independent. For each promoter sequence, a vector of size 132, with ...
cAMP response element (CRE)-binding protein-like-2 (CREBL2) was identified in a search to find genes in a commonly deleted region on chromosome 12p13 flanked by ETV6 and CDKN1B genes, frequently associated with hematopoietic malignancies, as well as breast, non-small-cell lung and ovarian cancers. CREBL2 shares a 41% identity with CRE-binding protein (CREB) over a 48-base long region which encodes the bZip domain of CREB. The bZip domain consists of about 30 amino acids rich in basic residues involved in DNA binding, followed by a leucine zipper motif involved in protein dimerization. This suggests that CREBL2 encodes a protein with DNA binding capabilities. The occurance of CREBL2 deletion in malignancy suggests that CREBL2 may act as a tumor suppressor gene.
Abuse of opiates, including morphine, induced remarkable synaptic adaptation in several brain regions including ventral tegmental area (VTA), which underlay the induction and maintenance of opioid dependence and addiction. Scaffolding protein postsynaptic density protein 95 (PSD-95) is critically involved in the glutamatergic synaptic maturation and plasticity in the central neurons. The present study revealed a significantly increased mRNA and protein expression of PSD-95 in the VTA of the rats conditioned with morphine. The further chromatin immunoprecipitation study found an increased histone H3 acetylation in the promoter region of Dlg4. An upregulation of expression of phosphorylated cAMP response element-binding protein (pCREB) and the occupancy of pCREB in the Dlg4 promoter region were shown in the VTA of the morphine-conditioned rats. Inhibition of pCREB activity significantly decreased the histone H3 acetylation in Dlg4 promoter region, PSD-95 upregulation, enhancement of glutamatergic strength
CREB3L1 antibody [C3], C-term (cAMP responsive element binding protein 3-like 1) for WB. Anti-CREB3L1 pAb (GTX104818) is tested in Human samples. 100% Ab-Assurance.
Early odor preference learning in rats is associated with increases of phosphorylated CREB (pCREB) in mitral cells of the olfactory bulb. In the present study, herpes simplex virus expressing CREB (HSV-CREB) and dominant-negative mutant CREB (HSV-mCREB) have been injected into the bulb to assess a causal role for CREB and pCREB in this model. Odor paired with stroking or with the β-adrenoceptor agonist isoproterenol produces odor approach 24 hr later. Isoproterenol-induced learning exhibits an inverted U curve dose-dependent learning relationship with both low and high doses failing to produce learning. pCREB increases have only been seen at the learning effective dose. In the present study, injection of an HSV vector expressing mutant CREB into the olfactory bulb prevented learning induced by stroking. Control HSV expressing LacZ was without effect. Expression of mutant CREB shifted the dose-learning curve for isoproterenol to the right such that a higher dose was required to induce learning. ...
Incubation of 3T3-L1 preadipocytes with isobutylmethylxanthine (IBMX), dexamethasone, and insulin, alone or in combination, demonstrated that IBMX, which increased cAMP-response element-binding protein (CREB) phosphorylation, was the predominant regulator of Pde3b expression. Real time PCR and immunoblotting indicated that in 3T3-L1 preadipocytes, IBMX-stimulated induction of Pde3b mRNA and protein was markedly inhibited by dominant-negative CREB proteins. By transfecting preadipocytes, differentiating preadipocytes, and HEK293A cells with luciferase reporter vectors containing different fragments of the 5- flanking region of the Pde3b gene, we identified a distal promoter that contained canonical cis-acting cAMP-response elements (CRE) and a proximal, GC-rich promoter region, which contained atypical CRE. Mutation of the CRE sequences dramatically reduced distal promoter activity; H89 inhibited IBMX-stimulated CREB phosphorylation and proximal and distal promoter activities. Distal promoter ...
The concentration of glucose in the bloodstream is regulated by glucose itself, along with the hormones insulin and glucagon. Glucagon stimulates gluconeogenesis in part by regulating phosphorylation of a transcriptional coactivator known as cyclic adenosine monophosphate response element-binding protein 2 (CRTC2). Dentin et al. (see the Perspective by Birnbaum) found that high concentrations of circulating glucose also regulate CRTC2, but do so through stimulation of the hexosamine biosynthetic pathway and consequent O-linked glycosylation of the same serine residue in CRTC2 that is modified by phosphorylation. Thus, CRTC2 integrates signals from hormones and nutrients and might be a target for efforts to treat abnormalities of glucose homeostasis that are associated with diabetes.. R. Dentin, S. Hedrick, J. Xie, J. Yates, III, M. Montminy, Hepatic glucose sensing via the CREB coactivator CRTC2. Science 319, 1402-1405 (2008). [Abstract] [Full Text]. M. J. Birnbaum, Sweet conundrum. Science 319, ...
Next, we focused on intermediate signaling molecules that are implicated in the mediation of cell motility and calcium signaling. Compared with control cells, MECs treated with recMFAP5 had higher expression of phosphorylated FAK (p-FAK) (Y861), p-PLC-γ1 (Y783), p-PKCθ (T538), p-ERK1/2 (T202/Y204), phosphorylated myosin regulatory light chain 2 (p-MLC2) (T18/S19), phosphorylated cyclic AMP-responsive element-binding protein (p-CREB) (S133), c-Jun, and p-c-Jun (S73), which may have led to the upregulation of LPP expression and thus increased cell motility and permeability (Figure 7G).. Because our data demonstrated that MFAP5-induced microvascular endothelial cell motility was suppressed in cells that had been pretreated with an anti-αVβ3 integrin antibody (Figure 7H) and that MFAP5-upregulated p-FAK (Y861) expression was suppressed in cells that had been pretreated with BAPTA-AM (1,2-bis-[2-aminophenoxy]-ethane-N,N,N′,N′-tetraacetic acid, tetraacetoxymethyl ester) (Supplemental Figure 9A ...
Rabbit anti CREB1 antibody recognizes cyclic AMP-responsive element-binding protein 1 (CREB1). CREB1 is a transcription factor that has be
Defective heme synthesis in mammals has been suspected of causing neuropathy associated with porphyrias and lead poisoning. To determine the molecular action of heme in neuronal cells, we examined the effect of the inhibition of heme synthesis on nerve growth factor (NGF) signaling in PC12 cells. We found that the inhibition of heme synthesis by succinyl acetone interferes with NGF-induced neurite outgrowth in PC12 cells. Furthermore, we show that heme deficiency obliterates the activation of the signaling intermediates of the Ras-mitogen-activated protein kinase signaling pathway and its downstream target, the transcription activator cyclic AMP response element-binding protein. Strikingly, microarray expression analysis shows that the inhibition of heme synthesis selectively diminishes the induction of expression of a subset of neuron-specific genes by NGF, such as Ras and neurofilament proteins, whereas NGF induces the expression of several major classes of neuronal genes that encode ...
Our hypothesis is based on several lines of evidence. First, the effects of cAMP and DA on both 4xCRE (Figs. 3a,b, 5) and c-fos and BDNF mRNA expression (Fig. 6) are blocked by NMDAR antagonists. Second, two structurally distinct inhibitors of neuronal EAA uptake, TBOA (Fig. 7) and trans-PDC (supplemental Fig. S3, available at www.jneurosci.org as supplemental material), potentiated the stimulation of gene transcription by cAMP. Third, the aspartate+glutamate-, but not the glutamate-only-, scavenging system abolished stimulation of CREB-dependent gene transcription by forskolin (Fig. 8); the aspartate-scavenging enzyme, GOT, degrades l- but not d-aspartate demonstrating that l-aspartate is the active extracellular EAA in this signaling pathway. Finally, forskolin was found to induce release of aspartate but not glutamate (Fig. 9). Together, these results lead to the conclusion that cAMP-induced release of aspartate and the resulting activation of NR2B-containing NMDARs mediate the effects of ...
In type 2 diabetes, chronic hyperglycemia is detrimental to beta-cells, causing apoptosis and impaired insulin secretion. The transcription factor cAMP-responsive element-binding protein (CREB) is crucial for beta-cell survival and function. We inves
CG-001 is a selective Wnt/β-catenin signalling inhibitor with an IC50 of 3μM. ICG 001, a small molecule that down-regulates beta-catenin/T cell factor signaling by specifically binding to cyclic AMP response element-binding protein. ICG001 selectively ind
RefSeq Summary (NM_001675): This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011 ...
Alzheimers disease (AD) is a progressive neurodegenerative disease and the most common form of senile dementia. Recently, scientists have put significant effort into exploring the molecular mechanisms involved in the pathological processes leading to the disease. A vast number of studies have focused on understanding the nitric oxide (NO) signaling pathway, which culminates with the phosphorylation of the transcription factor cAMP-responsive element-binding protein (CREB) through the increase of the second messenger cyclic guanosine monophosphate (cGMP) and activation of cGMP-dependent protein kinase. This book chapter provides an overview of the progress being made in modulating the hippocampal synaptic transmissions, which are critical for learning and memory, by targeting the different components of the NO/cGMP/CREB phosphorylation signaling pathway. Furthermore, a description of recent research on this pathway through the use of phosphodiesterase inhibitors is emphasized.
Colorectal cancer (CRC) is the third leading cause of cancer-related death in the United States. During the tumorigenesis and metastasis of CRC, cells encounter numerous cellular and molecular events. ATF3, a member of the ATF/CREB transcription factor family, plays an important role on regulation of apoptosis and is regarded as a potential molecular target for chemoprevention and chemotherapy of colon cancer. The current study was performed to investigate cellular and molecular mechanisms by which ATF3 affects colon cancer-related phenotypes including apoptosis and metastasis. Here, we demonstrated that knockdown of ATF3 using small interfering RNA (siRNA) promotes the expression of anti-apoptotic protein, B-cell lymphoma 2 (Bcl-2), in colon cancer cells, while overexpression of ATF3 resulted in a dramatic decrease in Bcl-2 protein. Gain of function of ATF3 in colon cancer cell line HCT116 led to an increase of pro-apoptotic protein Bcl-2 homologous antagonist killer (Bak), followed by the ...
We previously demonstrated that APP epigenetically regulates Egr1 expression both in cultured neurons and in vivo. Since Egr1 is an immediate early gene involved in memory formation, we wondered whether other early genes involved in memory were regulated by APP and we studied molecular mechanisms involved. By comparing prefrontal (PF) cortex from wild type (APP+/+) and APP knockout mice (APP-/-), we observed that APP down regulates expression of four immediate early genes, Egr1, c-Fos, Bdnf and Arc. Down regulation of Egr1, c-Fos and Bdnf transcription resulted from a decreased enrichment of acetylated histone H4 on the corresponding gene promoter. Further characterization of H4 acetylation at Egr1 and c-Fos promoters revealed increased acetylation of H4K5 and H4K12 residues in APP-/- mice. Whereas APP affected Egr1 promoter activity by reducing access of the CREB transcription factor, its effect on c-Fos appeared to depend on increased recruitment of HDAC2 histone deacetylase to the gene ...
TY - JOUR. T1 - Interplay of the E box, the cyclic AMP response element, and HTF4/HEB in transcriptional regulation of the neurospecific, neurotrophin-inducible vgf gene. AU - Di Rocco, Giuliana. AU - Pennuto, Maria. AU - Illi, Barbara. AU - Canu, Nadia. AU - Filocamo, Gessica. AU - Trani, Eugenia. AU - Rinaldi, Anna Maria. AU - Possenti, Roberta. AU - Mandolesi, Georgia. AU - Sirinian, M. Isabella. AU - Jucker, Richard. AU - Levi, Andrea. AU - Nasi, Sergio. PY - 1997/3. Y1 - 1997/3. N2 - vgf is a neurotrophin response-specific, developmentally regulated gene that codes for a neurosecretory polypeptide. Its transcription in neuronal cells is selectively activated by the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor, and neurotrophin 3, which induce survival and differentiation, and not by epidermal growth factor. We studied a short region of the rat vgf promoter which is essential for its regulated expression. A cyclic AMP response element (CRE) within this region is ...
article{955774, abstract = {Among the mitogen-activated protein kinase (MAPK) targets, MSKs (mitogen- and stress-activated protein kinases) comprise a particularly interesting protein family. Because MSKs can be activated by both extracellular-signal-regulated kinase and p38 MAPKs, they are activated by many physiological and pathological stimuli. About ten years after their original discovery, they have been recognized as versatile kinases regulating gene transcription at multiple levels. MSKs directly target transcription factors, such as cAMP-response-element-binding protein and nuclear factor-kappaB, thereby enhancing their transcriptional activity. They also induce histone phosphorylation, which is accompanied by chromatin relaxation and facilitated binding of additional regulatory proteins. Here, we review the current knowledge on MSK activation and its molecular targets, focusing on recent insights into the role of MSKs at multiple levels of transcriptional regulation.}, author = ...
In addition to these posttranslational changes, an alteration in the expression of effector molecules in the DRG (66) and dorsal horn (67-69) is a prominent feature of inflammation and can be initiated in two ways. The first is as a result of an activity-dependent activation of the CREB transcription factor both in DRG and dorsal horn neurons (ref. 70; Fig. 3). As discussed above, this will result, after a delay of several hours for transcription and translation in the DRG and protein transport to central terminals, in a potentiated system in which the C fibers are primed to exert a greater effect on dorsal horn neurons as a result of an increased expression of neuromodulators like BDNF. In addition, the dorsal horn is simultaneously made hyperresponsive to such neuromodulators as a result of an activity-dependent increased expression of the TrkB receptor. The second way that transcriptional changes occur after inflammation is via the production in the inflamed tissue of specific signal ...
negative regulation of gene expression / positive regulation of CREB transcription factor activity / G-protein coupled receptor signaling pathway / regulation of sensory perception of pain / protein import into nucleus, translocation / phospholipase C-activating G-protein coupled receptor signaling pathway / cellular response to growth factor stimulus / immune response / adult locomotory behavior / G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / neuropeptide signaling pathway / adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway / synaptic transmission / regulation of calcium ion transport / cellular response to toxic substance / eating behavior / cellular response to hypoxia / negative regulation of protein oligomerization / positive regulation of peptidyl-serine phosphorylation / regulation of mitochondrial membrane potential / opioid receptor signaling pathway ...
Extensive evidence implicates CREB-dependent gene transcription in memory (Bourtchuladze et al., 1994; Yin et al., 1994; Guzowski and McGaugh, 1997; Bartsch et al., 1998; Kida et al., 2002; Pittenger et al., 2002; Frankland et al., 2004). Multiple signaling pathways phosphorylate CREB at Ser133 (Shaywitz and Greenberg, 1999; Mayr and Montminy, 2001; Lonze and Ginty, 2002), which stimulates the recruitment of coactivators CBP/p300 (Chrivia et al., 1993; Parker et al., 1996). However, this phosphorylation event is not always sufficient to activate transcription (Impey et al., 1996; Mayr and Montminy, 2001) suggesting that CREB-mediated transcription is regulated by additional mechanisms.. In 2003, two laboratories identified a new family of CREB-specific coactivators, now referred to as CRTCs (Iourgenko et al., 2003; Conkright et al., 2003b). CRTC is thought to enhance transcription by facilitating the interaction of CREB with the RNA polymerase II pre-initiation complex (Conkright et al., 2003b; ...
TY - JOUR. T1 - Epistatic interaction of CREB1 and KCNJ6 on rumination and negative emotionality. AU - Lazary, Judit. AU - Juhasz, Gabriella. AU - Anderson, Ian M.. AU - Jacob, Christian P.. AU - Nguyen, T. Trang. AU - Lesch, Klaus Peter. AU - Reif, Andreas. AU - Deakin, J. F.William. AU - Bagdy, Gyorgy. PY - 2011/1/1. Y1 - 2011/1/1. N2 - G protein-activated K+ channel 2 (GIRK2) and cAMP-response element binding protein (CREB1) are involved in synaptic plasticity and their genes have been implicated depression and memory processing. Excessive rumination is a core cognitive feature of depression which is also present in remission. High scores on the Ruminative Response Scale (RRS) questionnaire are predictive of relapse and recurrence. Since rumination involves memory, we tested the hypothesis that variation in the genes encoding GIRK2 (KCNJ6) and CREB1 mechanisms would influence RRS scores. GIRK2 and CREB1 polymorphisms were studied in two independent samples (n = 651 and n = 1174) from the ...
The cellular transcription factor CREB (cAMP response element-binding protein) helps learning and the stabilization and retrieval of fear-based, long-term memories. This is done mainly through its expression in the hippocampus and the amygdala. Studies supporting the role of CREB in cognition include those that knock out the gene, reduce its expression, or overexpress it. Research suggests that CREB has a role in the molecular steps that stabilize memory in the brain, including that of emotional memory. Evidence of CREBs role in emotional memory falls into three experimental categories: negative manipulations (where the levels of CREB were lowered), positive manipulations (where the levels of CREB were increased), and non-interventions (where the endogenous levels of CREB were tracked before and after learning). Knockout studies in Aplysia sea slugs indicated that decreasing CREB function blocks long-term changes in synaptic function, but not short-term ones. Changes in synaptic function (i.e., ...
Many human and murine tumors of distinct histology and cells of the tumor microenvironment show increased CREB expression and activity when compared with adjacent nonmalignant tissue (4), which is even more enhanced in metastasis suggesting a role for CREB in the initiation, maintenance, and progression of tumors (33, 34). This is often accompanied by poor prognosis and decreased survival rates of patients with tumor (35). Although CREB dysregulation is a critical factor in cancer development and progression, the underlying molecular mechanisms that lead to its overexpression have not yet been determined in detail. One approach to unravel this process is to determine the link(s) between oncogene expression and CREB using an in vitro model of oncogene-mediated transformation, as well as human tumor lesions with known HER-2/neu status. As shown in Fig. 7F, an important role for HER-2/neu was suggested for CREB expression and activity both in vitro and in vivo. This is based on the data ...
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TY - JOUR. T1 - CREB activation in the rapid, intermediate, and delayed ischemic preconditioning against hypoxic-ischemia in neonatal rat. AU - Lin, Wan Ying. AU - Chang, Ying Chao. AU - Lee, Hsueh Te. AU - Huang, Chao Ching. PY - 2009/2. Y1 - 2009/2. N2 - Ischemic preconditioning (IP) is a defense program in which exposure to sublethal ischemia followed by a period of reperfusion results in subsequent resistance to severe ischemic insults. Very few in vivo IP models have been established for neonatal brain. We examined whether rapid, intermediate, and delayed IP against hypoxic-ischemia (HI) could be induced in neonatal brain, and if so, whether the IP involved phosphorylation of cAMP response element-binding protein (pCREB) after HI. Postnatal day 7 rat pups were subjected to HI at 2 h (2-h IP), 6 h (6-h IP), or 22 h (22-h IP) after IP. We found all three IP groups had significantly reduced neuronal damage and TUNEL-(+) cells 24 h post-HI than no-IP group. Compared with control, the no-IP ...
Ca2+ release induced by depolarization of skeletal muscle cells can be separated into two independent components; the fast Ca2+ transient, homogenously distributed through the cell that corresponds to excitation-contraction coupling, and the slow Ca2+ transient, with a distinct nuclear component, generated by IP3 (Jaimovich et al., 2000). Slow Ca2+ signals with similar characteristics, although displaying particular kinetics can also be elicited by androgens like testosterone (Estrada et al., 2000; Estrada et al., 2003), or hormones such as insulin-like growth factor (Espinosa et al., 2004). Slow Ca2+ signals have been shown to participate in phosphorylation of both MAP kinases ERK 1 and 2, and transcription factor CREB, as well as in the expression of early genes fos, jun and egr-1 (Powell et al., 2001; Carrasco et al., 2003). In this work, we determined that type 1 and 3 IP3Rs are expressed and are functional in myonuclei, mediating nucleoplasmic Ca2+ changes, which in turn induce CREB ...
Co-localization of p-CREB and GFAP in coronal brain sections of ACC. Photomicrographs showed the expression of p-CREB (red) and GFAP (an astrocytic marker, gree
View Notes - 2010 Bio 317 Lecture 24 from BIO 317 at SUNY Stony Brook. 12/1/10 CREB on CRE Alphahelix LeucineZipper MG2+ Attenuation Due to S/T Phosphatase PP-1 PassiveDiffusion 1 12/1/10 CBP -
The general public might think that this is only possible in the movie or in the distant future (Y 2084). However, in reality, a research team led by professor Jin-Hee Han at KAIST reported that a substance or chemical can be injected into the mouse brain to induce recall artificially or make modifications of specific memory in the absence of any behavioural cues (Kim et al., Nature Neuroscience 2014). It has been reported that neurons with higher level of CREB than their neighbours (CREB = cAMP/Ca2+ response element binding protein) are selected to be included into memory engram during memory formation. Inspired by this finding, the CREB gene was injected into small number of cells in the mouse amygdala, a brain area responsible for fear. Surprisingly, simply injecting the drug that stimulated only CREB cells was sufficient to activate recall of that learned memory. Moreover, the same procedure was also able to modify the established memory. This was laboratory work carried out by the leading ...
Maio M, Bertocci E, Pilla L, Fazio C, Chiarucci C, Cutaia O, Fonsatti E, Maccalli C, Parmiani G; NIBIT. (Seliger B). Cancer Bio-Immunotherapy in Siena: Twelfth Meeting of the Network Italiano per la Bioterapia dei Tumori (NIBIT), Siena, Italy, October 9-11, 2014. Cancer Immunol Immunother. 2016 Jan;65(1):119-26. Rothe K, Quandt D, Schubert K, Rossol M, Klingner M, Jasinski-Bergner S, Scholz R, Seliger B, Pierer M, Baerwald C, Wagner U. Latent Cytomegalovirus Infection in Rheumatoid Arthritis and Increased Frequencies of Cytolytic LIR-1+CD8+ T Cells. Arthritis Rheumatol. 2016 Feb;68(2):337-46. Lennicke C, Rahn J, Heimer N, Lichtenfels R, Wessjohann LA, Seliger B. Redox proteomics: Methods for the identification and enrichment of redox-modified proteins and their applications. Proteomics. 2016 Jan;16(2):197-213. Steven A, Seliger B. Control of CREB expression in tumors: from molecular mechanisms and signal transduction pathways to therapeutic target. Oncotarget. 2016 Jun 7;7(23):35454-65. ...
Maio M, Bertocci E, Pilla L, Fazio C, Chiarucci C, Cutaia O, Fonsatti E, Maccalli C, Parmiani G; NIBIT. (Seliger B). Cancer Bio-Immunotherapy in Siena: Twelfth Meeting of the Network Italiano per la Bioterapia dei Tumori (NIBIT), Siena, Italy, October 9-11, 2014. Cancer Immunol Immunother. 2016 Jan;65(1):119-26. Rothe K, Quandt D, Schubert K, Rossol M, Klingner M, Jasinski-Bergner S, Scholz R, Seliger B, Pierer M, Baerwald C, Wagner U. Latent Cytomegalovirus Infection in Rheumatoid Arthritis and Increased Frequencies of Cytolytic LIR-1+CD8+ T Cells. Arthritis Rheumatol. 2016 Feb;68(2):337-46. Lennicke C, Rahn J, Heimer N, Lichtenfels R, Wessjohann LA, Seliger B. Redox proteomics: Methods for the identification and enrichment of redox-modified proteins and their applications. Proteomics. 2016 Jan;16(2):197-213. Steven A, Seliger B. Control of CREB expression in tumors: from molecular mechanisms and signal transduction pathways to therapeutic target. Oncotarget. 2016 Jun 7;7(23):35454-65. ...
20E transmits signals through the ErGPCR-cAMP-PKA-CREB signaling pathway prior to the initiation of the gene expression through the EcR/UPS pathway
Sigma-Aldrich offers abstracts and full-text articles by [Shou-Peng Fu, Wei Wang, Bing-Run Liu, Huan-Min Yang, Hong Ji, Zhan-Qing Yang, Bin Guo, Ju-Xiong Liu, Jian-Fa Wang].
Exactly how TCL1 influences the behaviour of CLL cells was made clearer by another paper by Pekarsky et al (PNAS 2008; 105:19643-8). In contrast to T-PLL, the AKT oncoprotein does not seem to be involved. The cAmp Response Element Binding (CREB) protein, p300, is a transcription activator involved in transactivation mediated by several signalling pathways including NF-kappaB. Co-precipitation experiments showed that TCL1 interacts with p300. However, sequence analysis demonstrated two mutant sequences of TCL1 among the CLL patients studied. These mutant forms of TCL1 did not upregulate the NF-kappaB pathway to the same extent as wild type TCL1, leading the investigators to expect that another pathway might also be involved. One of the best known pathways involving p300 is activator protein 1 (AP-1) -dependent transcription. This complex contains c-Jun and c-Fos. AP-1 induces apoptosis by transactivating proapoptotic genes. Wild type TCL1 inhibits AP-1 dependent transactivation ~ 2.5 fold, ...
The AlphaLISA SureFire Ultra p-CREB (Ser133) assay kit (High Volume) is an immunoassay for quantitative detection of phospho-CREB in cellular lysates.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
A-D: Phospho-CREB-positive cells are increased after beraprost treatment. (7A and 7C). Representative images of brain sections show p-CREB immunostaining in Y
Plasmid pFETCH_CREB1 from Dr. Eric Mendenhalls lab contains the insert CREB1 Homology Arms and is published in Genome Res. 2015 Sep 9. This plasmid is available through Addgene.
O-linked â-N-acetylglucosamine is a regulatory post translational modification. This modification occurs on nearly all functional classes of proteins, in the nucleus and cytoplasm. O-GlcNAc is added to serine or threonine by O-GlcNAc transferase and removed by O-GlcNAcase. Previous attempts to study O-GlcNAc-modified proteins have resulted in low yields, making 3-dimensional structure determination impossible. In this dissertation O-GlcNAc transferase will be co-expressed with domains of human cAMP responsive element-binding protein (CREB1) and Abelson tyrosine-kinase 2 (ABL2) in E. coli, to produce O-GlcNAc-modified protein. The O-GlcNAc-modified protein was expressed in a variety of E. coli cell lines at a variety of conditions, but only small quantities of insoluble protein were produced. A glycosidase was suspected due to the disappearance of the O-GlcNAc modification from the protein. O-(2-acetamido-2-dexoy-dglucopyranosylidene) amino-N-phenylcarbamate (PUGNAc), a â-N-acetylglucosaminidase
We have shown previously that chronic administration of adrenocorticotropic hormone (ACTH) causes a significant decrease in hippocampal cell proliferation and neurogenesis. This effect in rats treated chronically with ACTH was not influenced by the chronic administration of imipramine, but was reversed by coadministration of imipramine and lithium. The present study was undertaken to further characterize the mechanism underlying the effect of imipramine and lithium on hippocampal cell proliferation and neurogenesis, by investigating the effects of treatment on the expression of brain-derived neurotrophic factor (BDNF), total cyclic adenosine monophosphate response element-binding protein (CREB), and phosphorylated CREB (pCREB) of the CREB signaling system, as well as Wnt 3a and cyclin D1 of the Wnt signaling pathway in the hippocampus of saline- and ACTH-treated rats. ACTH treatment significantly decreased the expression of cyclin D1. Treatment with imipramine and lithium increased the expression of
PDZ-LIM proteins form a family of the scaffolding protein essential for both embryonic and post-natal development. ENH1 (PDLIM5) is a PDZ-LIM protein, composed of an N-terminal PDZ domain and 3 LIM domains at the C-terminal end. The enh gene encodes for several splice variants that have opposite functions. ENH1 promotes the cardiomyocytes hypertrophy whereas ENH splice variants lacking LIM domains prevents it. At the molecular level, ENH1 interacts with Protein kinase C (PKC) and Protein Kinase D1 (PKD1) both kinases playing a pivotal role in the pathological remodeling of the heart. In addition, the binding of ENH1s LIM domains to PKC is sufficient to activate the kinase without any stimulation. However, the downstream events of the ENH1-PKC/PKD1 complex remain unknown. PKC and PKD1 are known to phosphorylate the transcription factor cAMP-response element binding protein (CREB) in cardiomyocytes. We therefore hypothesized that ENH1 could be a play a role in the PKC/PKD1-dependent activation of ...
Abbreviations: Ago2, Argonaute 2; ARE, AU-rich element; ASK1, apoptosis signal-regulating kinase 1; ATF, activating transcription factor; BAF60, BRG1-associated factor 60; CDK, cyclin-dependent kinase; C/EBP, CCAAT/enhancer-binding protein; c-IAP1/2, cellular inhibitor of apoptosis 1/2; CREB, cAMP-response-element-binding protein; CSBP, cytokine-suppressive anti-inflammatory drug-binding protein; DDB2, damaged-DNA-binding complex 2; D domain, docking domain; EGFR, epidermal growth factor receptor; ERK, extracellular-signal-regulated kinase; FADD, Fas-associated death domain; FGFR1, fibroblast growth factor receptor 1; FLIPs, short isoform of FLICE (FADD-like interleukin 1β-converting enzyme)-inhibitory protein; GRK2, G-protein-coupled receptor kinase 2; GSK, glycogen synthase kinase; hDlg, human discs large; HePTP, haemopoietic tyrosine phosphatase; IKK, IκB (inhibitor of nuclear factor κB) kinase; IL, interleukin; JNK, c-Jun N-terminal kinase; JIP, JNK-interacting protein; JLP, ...
The major finding in this study is that ethanol induces an increase in gene expression via CREB and PKA. This increase in gene expression requires both PKA and CREB phosphorylation. Although we had previously shown that exposure to ethanol resulted in phosphorylation of CREB in NG108-15 cells, there is accumulating evidence that CREB phosphorylation is not sufficient to regulate gene expression under the control of CREs; activation of the coactivator CREB-binding protein (CBP) and other downstream elements is also required for increases in CRE-mediated gene expression (Montminy, 1997; Cardinaux et al., 2000).Thibault et al. (2000) have reported increases in genes the expression of which is known to be cAMP-dependent. However, ethanol activates many different signal transduction pathways in addition to PKA (Diamond and Gordon, 1997), and most genes have regulatory elements activated or inhibited by all of these pathways. Therefore, the experiments presented here are the first demonstration that ...
The 86-kDa IE2 protein (IE86) of human cytomegalovirus (HCMV) has been described as a promiscuous transactivator of viral, as well as cellular, gene expression. Investigation of the mechanism used by IE86 to activate gene expression from the early UL112/113 promoter of HCMV revealed the existence of three binding sites for IE86 located between nucleotides -290 and -120 relative to the transcriptional start site (H. Arlt, D. Lang, S. Gebert, and T. Stamminger, J. Virol. 68:4117-4125, 1994). As shown previously, deletion of these target sites resulted in a reduction of IE86-mediated transactivation by approximately 70%. The remaining promoter, however, could still be stimulated about 40-fold, indicating the presence of an additional responsive element within these sequences. Here, we provide evidence that a binding site for the cellular transcription factor CREB can also act as a target for IE86 transactivation. By DNase I protection analysis, a binding sequence for CREB could be detected between ...
TY - JOUR. T1 - Transcriptional profiling of PPARα−/− and CREB3L3−/− livers reveals disparate regulation of hepatoproliferative and metabolic functions of PPARα. AU - Ruppert, Philip M.M.. AU - Park, Jong-Gil. AU - Xu, Xu. AU - Hur, Kyu Yeon. AU - Lee, Ann-Hwee. AU - Kersten, Sander. PY - 2019/3/11. Y1 - 2019/3/11. N2 - Background: Peroxisome Proliferator-Activated receptor α (PPARα) and cAMP-Responsive Element Binding Protein 3-Like 3 (CREB3L3) are transcription factors involved in the regulation of lipid metabolism in the liver. The aim of the present study was to characterize the interrelationship between PPARα and CREB3L3 in regulating hepatic gene expression. Male wild-type, PPARα−/−, CREB3L3−/− and combined PPARα/CREB3L3−/− mice were subjected to a 16-h fast or 4 days of ketogenic diet. Whole genome expression analysis was performed on liver samples. Results: Under conditions of overnight fasting, the effects of PPARα ablation and CREB3L3 ablation on plasma ...
c-Jun enhancement of cyclic adenosine 3,5-monophosphate response element-dependent transcription induced by transforming growth factor-beta is independent of c-Jun binding to DNA.
Although we demonstrated that pp90RSK is activated by GM-CSF and able to phosphorylate CREB in vitro, these results alone do not provide evidence that phosphorylation may occur in vivo. In an effort to link GM-CSF-induced pp90RSK activation and CREB phosphorylation toegr-1 expression, we performed transfection experiments using the egr-1 reporter construct and kinase-defective pp90RSK (RSK KD). We previously showed that the −116 CAT/egr-1promoter construct is induced 3-fold in response to GM-CSF stimulation in TF-1 cells, and that the CRE contained within this region is required for maximal transcriptional activation.9 The −116 nucleotide egr-1 promoter construct containing the chloramphenicol acetyltransferase (CAT) gene was cotransfected with expression vectors containing wild-type pp90RSK (RSK WT), kinase-defective (K112/464R) pp90RSK, or empty vector control pcDNA3. No significant differences in GM-CSF-induced reporter activity were observed on cotransfection of the −116 CAT egr-1 ...
Brain-derived neurotrophic factor (BDNF) is a key player in synaptic plasticity, and consequently, learning and memory. Because of its fundamental role in numerous neurological functions in the central nervous system, BDNF has utility as a biomarker and drug target for neurodegenerative and neuropsychiatric disorders. Here, we generated a screening assay to mine inducers of Bdnf transcription in neuronal cells, using primary cultures of cortical cells prepared from a transgenic mouse strain, specifically, Bdnf-Luciferase transgenic (Bdnf-Luc) mice. We identified several active extracts from a library consisting of 120 herbal extracts. In particular, we focused on an active extract prepared from Ginseng Radix (GIN), and found that GIN activated endogenous Bdnf expression via cAMP-response element-binding protein-dependent transcription. Taken together, our current screening assay can be used for validating herbal extracts, food-derived agents, and chemical compounds for their ability to induce Bdnf
Full Text - Ligands of nicotinic acetylcholine receptors (nAChRs) are widely considered as potential therapeutic agents. The present study used primary hippocampus cells and APPswe/PSEN1dE9 double-transgenic mice models to study the possible therapeutic effect and underlying mechanism of the specific activation of α7 nAChR by PNU-282987 in the pathogenesis of Alzheimer’s disease. The results indicated that activation of α7 nAChR attenuated the Aβ-induced cell apoptosis, decreased the deposition of Aβ, increased the expression of synaptic-associated proteins, and maintained synaptic morphology. Furthermore, in the APP/PS1_DT mice model, activation of α7 nAChR attenuated Aβ-induced synaptic loss, reduced the deposition of Aβ in the hippocampus, maintained the integral structure of hippocampus-derived synapse, and activated the calmodulin (CaM)-calmodulin-dependent protein kinase II (CaMKII)-cAMP response element-binding protein signaling pathway by
iHOP - Information Hyperlinked over Proteins. iHOP provides the network of genes and proteins as a natural way of accessing the millions of abstracts in PubMed. By employing genes and proteins as hyperlinks between sentences and abstracts, the information in PubMed becomes bound together into one navigable resource. A Gene Network for Navigating the Literature, Nature Genetics 36, 664 (2004). www.ihop-net.org/UniPub/iHOP/
Mitigating Ischemic Injury of Stem Cell-Derived Insulin-Producing Cells after Transplant Scientists showed that more than half of stem cell-derived insulin-producing cells (SCIPCs) die shortly after transplantation. Nutrient deprivation and hypoxia acted synergistically to kill SCIPCs in vitro. Amino acid supplementation rescued SCIPCs from nutrient deprivation, likely by providing cellular energy. [Stem Cell Reports] Full Article , Graphical Abstract Glucose Potentiates β-Cell Function by Inducing Tph1 Expression in Rat Islets Global gene expression patterns revealed that tryptophan hydroxylase 1 (Tph1) was the most profound of genes that are up-regulated in rat islets exposed to high glucose. Calcium and cAMP signals synergistically mediated glucose-stimulated Tph1 transcription in β-cells by activating cAMP-responsive element-binding protein and promoting its binding with a Tph1 promoter. [FASEB J] Abstract Differential Effects of Linagliptin on the Function of Human Islets Isolated from ...
Recently it has been demonstrated that catecholamines are produced and used by macrophages and mediate immune response. The aim of this study was to verify if endothelial cells (EC), that are of myeloid origin, can produce catecholamines. We demonstrated by Real Time PCR that genes coding for TH, DDC, DβH and PNMT, enzymes involved in the synthesis of catecholamines, are all expressed in basal conditions in bovine aorta EC (BAEC) and their expression is enhanced in response to 16 hours of hypoxia (fold of basal: TH:4.7 ± 0.15; DDC:3.9 ± 0.21; DβH:4.8 ± 0.23; PNMT:5.01 ± 0.01). This result was confirmed by western blot and immunohistochemical analysis. Moreover, hypoxia enhances norepinephrine (NE) and epinephrine (EPI) release respect to basal conditions (NE:+44,7±11,3; EPI:+51,6±6% of basal). In order to assess the signal transduction pathway that regulates catecholamines synthesis in EC, we overexpressed in BAEC either PKA or the transcription factor CREB, since PKA/CREB activation ...
As already mentioned, there are many other effects of cAMP. Lets have a look at another example. When there is an increase in cAMP levels, there is activation of transcription of various target genes in many animal cells. These target genes are known to contain the specific regulatory sequence called as the cAMP response element, generally abbreviated as CRE (diagramatic representation on the right side). Again, as described above, when cAMP binds to regulatory subunit of PKA, the catalytic subunit is released which carries the signal from cytoplasm to nucleus. Within the nucleus this activated PKA phosphorylates a transcription factor called CREB (CRE binding protein) at serine residue. This in turn recruits various co-activators and transcription of cAMP inducible genes takes place. This regulation of gene expression plays an important role in various processes like proliferation, differentiation, survival etc ...
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Targeting hepatic CREB to reduce glucose output as a treatment option for diabetes is a highly controversial notion. Inhibition of CREB activity in the liver us...
CREM - CREM (untagged)-Human cAMP responsive element modulator (CREM), transcript variant 20 available for purchase from OriGene - Your Gene Company.
CREB+CREM兔多克隆抗体(ab5803)可与小鼠, 大鼠, 鸡, 人样本反应并经WB, IHC, EMSA, ChIP, ICC/IF实验严格验证,被6篇文献引用并得到2个独立的用户反馈。
Background: cAMP response element-binding protein (CREB) is one of the cellular transcription factors found in neurons. CREB is also important for the survival of neurons, and has an important role in the development of drug addiction. Datura stramonium (DS) is a tropical ubiquitous plant commonly used to increase the intoxication of certain beverages for recreational purposes. The seeds of this plant are very toxic and may produce addiction on prolong usage. This research investigated the effects of administration of high doses of DS seeds on the expression of CREB protein in both male and female rats frontal cortices and its implication in addiction and neurodegeneration. Materials and Methods: The study was conducted with a total of 24 male and female Wistar rats weighing 200 g - 250 g. The rats were divided into three groups of 8 rats each. Each group was further divided into four sub-groups of 2 rats each. Ethanolic dried seed extract of DS was diluted in normal saline and administered
One of the major functions of the metal response element-binding transcription factor 1(MTF-1) is to sense and maintain sub-nanomolar to nanomolar zinc levels in response to influxes of labile zinc within the cell. MTF-1 responses to elevated zinc include up regulation of metallothionein (MT-I & II) and efflux transporter (ZnT1) genes. MTF-1 also responds to oxidative stress and heavy metal loads. Due to a lack of liver development, MTF-1 is essential for embryogenesis as determined from knockout mice. The zinc dependence of DNA-binding and interactions with other transcription factors has been identified as major determinants in the homeostatic regulation of labile intracellular zinc by MTF-1. p300 along with its paralog, cyclic-AMP response element binding protein (CBP), have histone acetyltransferase protein scaffold functions and interact with other transcription factors. Previous studies have shown that p300, Sp1 and MTF-1 form a complex. It was also found that a zinc dependent interaction ...
Glucagon is conventionally regarded as a counterregulatory hormone for insulin and plays a critical anti-hypoglycemic role by maintaining glucose homeostasis in both animals and humans. To increase blood glucose, glucagon promotes hepatic glucose output by increasing glycogenolysis and gluconeogenesis and by decreasing glycogenesis and glycolysis in a concerted fashion via multiple mechanisms. Glucagon also stimulates hepatic mitochondrial beta-oxidation to supply energy for glucose production. Glucagon performs its main effect via activation of adenylate cyclase. The adenylate-cyclase-derived cAMP activates protein kinase A (PKA), which then phosphorylates downstream targets, such as cAMP response element binding protein (CREB) and the bifunctional enzyme 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase (one of the isoforms being PFK/FBPase 1, encoded by PFKFB1 ...
Inhibition of phosphodiesterase-4 (PDE4), an enzyme that catalyzes the hydrolysis of cyclic AMP (cAMP), increases phosphorylation of the cAMP response element binding protein (pCREB) and hippocampal neurogenesis, and produces antidepressant-like effects on behavior; however, causal links among these actions have not been established. In this study, chronic administration of rolipram (0.31-1.25 mg/kg, 16-23 days) produced antidepressant- and anxiolytic-like effects on behavior in mice. It also increased cAMP and pCREB levels in the hippocampus and prefrontal cortex, but increased Sox2, a marker for mitotic progenitor cells, only in the hippocampus. Chronic rolipram treatment also increased hippocampal neurogenesis, as evidenced by increased bromodeoxyuridine (BrdU)-positive cells in the hippocampal dentate gyrus. Methylazoxymethanol (MAM), which is toxic to proliferating cells, reversed rolipram-induced increases in BrdU-positive cells and pCREB in the hippocampus and partially blocked its ...
Called "Alzheimers Disease Beyond Aβ," the Keystone Symposium held 10-15 January 2010 at Copper Mountain, Colorado, was convened to discuss therapeutic approaches to Alzheimer disease besides those that target amyloid-β (Aβ). Organized by Tony Wyss-Coray of Stanford University, California, and JoAnne McLaurin, University of Toronto, Canada, the meeting didnt truly get away from Aβ, but it did feature novel presentations on alternative approaches to treatment.. Sheena Josselyn, Hospital for Sick Children, Toronto, Canada, raised the prospect of targeting cAMP response element binding protein, or CREB for short, to boost memory. CREB is known to be a key player in the formation of new memories, with early work led by Nobel laureate Eric Kandel, Columbia University, amongst others, showing that knocking out the protein disrupts memory. While in Alcino Silvas lab at University of California, Los Angeles, Josselyn showed that poor memory in CREB knockout mice is not simply a result of ...
Transcriptional regulation in HCMV-infected cells relies on a complex interaction between cellular and viral transactivators (13, 23, 28, 37, 41, 43, 46). Several studies have implicated a role for the transcription factors ATF/CREB in early gene regulation (25, 30, 35, 37, 39). For example, several early promoters can be regulated by ATF/CREB sites in transient assays (30, 35, 37, 39). In addition, a role for ATF/CREB in the activation of the UL54 and UL112-113 promoters at early times in the context of the viral genome has been demonstrated (25,35). Our present analysis of the US11 promoter revealed that expression of this early gene is also regulated by two ATF/CREB sites within the promoter. The primary regulatory element of the US11 promoter, both in transient assays and in the context of the viral genome, is an ATF site located immediately upstream of the TATA element. In addition to the ATF site, the CREB site at −83 was also involved in US11 promoter activation. In the context of the ...
Smith-Magenis syndrome; DKFZp4 §§). A polymorphic CAG repeat* on the C terminus contains a polyserine stretch underlying a serine-to-asparagine change at amino acid 1808 (S1808N), of the analogous a pathway when dominant negative CREB proteins with mutations underwent cell death in the CaG* b channel. And 3 RAI1 domains are a dosage-sensitive gene and an active metabolite of vitamin A involved in RA-induced neuritogenesis, that is a natural morphogen involved in body weight control and complex behavioral responses with a zinc finger-like plant homeodomain (PHD) at the C terminus that is conserved in the trithorax group of chromatin-based transcription regulators between human and mouse Dp(11)17 orthologs. Alcohol teratogenesis may be due in part to retinoic acid-induced expression of GDE2 [GDPD5] glycerols that plays essential roles in neuronal differentiation and neurite outgrowth that proceeds in multiple processes. In the hydrolysis of deacylated glycerophospholipids to glycerol ...
PGE2, the major product of cyclooxygenases implicated in carcinogenesis, is significantly upregulated in cervical cancer. PGE2 via prostanoid receptor EP4 stimulates proliferation and motility while inhibiting apoptosis and immune surveillance. It promotes angiogenesis by stimulating the production of pro-angiogenic factors. The present study demonstrates GW627368X, a highly selective competitive EP4 antagonist, which hinders cervical cancer progression by inhibiting EP4/epithelial growth factor receptor (EGFR) interactive signaling. GW627368X reduced protein kinase A (PKA) phosphorylation which in turn leads to decreased cAMP response element-binding protein (CREB) activation. Decreased PKA phosphorylation also directly enhanced Bax activity and in part reduced glycogen synthase kinase 3 (GSK3)β phosphorylation. Owing to the interactive signaling between EP4 and EGFR, GW627368X lowered EGFR phosphorylation in turn reducing Akt, mitogen-activated protein kinase (MAPK) and GSK3β activity ...
3.0.CO;2-B. ISSN 1040-452X. PMID 10824972. Sassone-Corsi, P. (1998-08-01). "CREM: a master-switch governing male germ cells differentiation and apoptosis". Seminars in Cell & Developmental Biology. 9 (4): 475-482. doi:10.1006/scdb.1998.0200. ISSN 1084-9521. PMID 9813195. "CREM (cAMP responsive element modulator)". atlasgeneticsoncology.org. Retrieved 2016-10-16. Fimia GM, De Cesare D, Sassone-Corsi P (Nov 2000). "A family of LIM-only transcriptional coactivators: tissue-specific expression and selective activation of CREB and CREM". Molecular and Cellular Biology. 20 (22): 8613-22. doi:10.1128/MCB.20.22.8613-8622.2000. PMC 102166 . PMID 11046156. Fimia GM, De Cesare D, Sassone-Corsi P (Mar 1999). "CBP-independent activation of CREM and CREB by the LIM-only protein ACT". Nature. 398 (6723): 165-9. doi:10.1038/18237. PMID 10086359. Domschke, K.; Kuhlenbäumer, G.; Schirmacher, A.; Lorenzi, C.; Armengol, L.; DiBella, D.; Gratacos, M.; Garritsen, H. S.; Nöthen, M. M. (2003-02-01). "Human nuclear ...
The world was originally introduced to the concept of synaptic plasticity over 60 years ago, when Dr. Donald Hebb first clearly defined a physiological mechanism for learning and memory in his seminal work "The Organization of Behavior". It took another 20 years for Bliss and Lomo to scientifically validate Hebbs postulate, and show that neurons could alter their ability to communicate with one another in a persistent manner. Together, these works started off what has grown to become the field of synaptic plasticity. The years following the initial discovery were exciting times for learning and memory young researchers like myself, and each discovery over the next 20 years seemed to push us closer to elucidating the biological mechanisms responsible for memory formation. This seemed particularly true in the mid-1980s when the NMDA receptor was being heralded as the key to learning and memory processes. However, more recently it has become obvious that the activation of membrane receptors is ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Biologists have long pondered how animal cells regulate the composition of their complex lipid membranes. In their Perspective, Nohturfft and Losick discuss new work ( Dobrosotskaya et al.) that solves another piece of the puzzle. Flies and mammals share a similar feedback pathway for lipid synthesis that depends on controlling the proteolysis of sterol response element binding protein. The new work shows that in flies, cleavage of this protein depends on a membrane phospholipid sensor, phosphatidylethanolamine. ...
ATF-4兔多克隆抗体(ab23760)可与人样本反应并经WB, ICC/IF实验严格验证,被7篇文献引用。所有产品均提供质保服务,中国75%以上现货。
Alcoholism, also called dependence on alcohol (ethanol), is a chronic relapsing disorder that is progressive and has serious detrimental health outcomes. As one of the primary mediators of the rewarding effects of alcohol, dopaminergic ventral tegmental area (VTA) projections to the nucleus accumbens (NAc) have been identified. Acute exposure to alcohol stimulates dopamine release into the NAc, which activates D1 receptors, stimulating PKA signaling and subsequent CREB-mediated gene expression, whereas chronic alcohol exposure leads to an adaptive downregulation of this pathway, in particular of CREB function. The decreased CREB function in the NAc may promote the intake of drugs of abuse to achieve an increase in reward and thus may be involved in the regulation of positive affective states of addiction. PKA signaling also affects NMDA receptor activity and may play an important role in neuroadaptation in response to chronic alcohol exposure ...
Iron is required for normal cell growth and proliferation. However, excess iron is potentially harmful, as it can catalyse the formation of toxic reactive oxygen species (ROS) via Fenton chemistry. For this reason, cells have evolved highly regulated mechanisms for controlling intracellular iron levels. Chief among these is the sequestration of iron in ferritin. Ferritin is a 24 subunit protein composed of two subunit types, termed H and L. The ferritin H subunit has a potent ferroxidase activity that catalyses the oxidation of ferrous iron, whereas ferritin L plays a role in iron nucleation and protein stability. In the present study we report that increased synthesis of both subunits of ferritin occurs in HeLa cells exposed to oxidative stress. An increase in the activity of iron responsive element binding proteins in response to oxidative stress was also observed. However, this activation was transient, allowing ferritin protein induction to subsequently proceed. To assess whether ferritin ...
Dietary iron supplementation is associated with increased appetite. Here, we investigated the effect of iron on the hormone leptin, which regulates food intake and energy homeostasis. Serum ferritin was negatively associated with serum leptin in a cohort of patients with metabolic syndrome. Moreover, the same inverse correlation was observed in mice fed a high-iron diet. Adipocyte-specific loss of the iron exporter ferroportin resulted in iron loading and decreased leptin, while decreased levels of hepcidin in a murine hereditary hemochromatosis (HH) model increased adipocyte ferroportin expression, decreased adipocyte iron, and increased leptin. Treatment of 3T3-L1 adipocytes with iron decreased leptin mRNA in a dose-dependent manner. We found that iron negatively regulates leptin transcription via cAMP-responsive element binding protein activation (CREB activation) and identified 2 potential CREB-binding sites in the mouse leptin promoter region. Mutation of both sites completely blocked the ...
1. ChoH, MuJ, KimJK, ThorvaldsenJL, ChuQ, et al. (2001) Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science 292: 1728-1731.. 2. TaniguchiCM, KondoT, SajanM, LuoJ, BronsonR, et al. (2006) Divergent regulation of hepatic glucose and lipid metabolism by phosphoinositide 3-kinase via Akt and PKClambda/zeta. Cell Metab 3: 343-353.. 3. WanM, LeavensKF, SalehD, EastonRM, GuertinDA, et al. (2011) Postprandial hepatic lipid metabolism requires signaling through Akt2 independent of the transcription factors FoxA2, FoxO1, and SREBP1c. Cell Metab 14: 516-527.. 4. SaltielAR, KahnCR (2001) Insulin signalling and the regulation of glucose and lipid metabolism. Nature 414: 799-806.. 5. AriasJ, AlbertsAS, BrindleP, ClaretFX, SmealT, et al. (1994) Activation of cAMP and mitogen responsive genes relies on a common nuclear factor. Nature 370: 226-229.. 6. ChriviaJC, KwokRP, LambN, HagiwaraM, MontminyMR, et al. (1993) Phosphorylated CREB binds ...
CREM - Lenti-ORF clone of CREM (Myc-DDK-tagged)-Human cAMP responsive element modulator (CREM), transcript variant 10 available for purchase from OriGene - Your Gene Company.
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Under fasting conditions, increases in circulating concentrations of glucagon maintain glucose homeostasis via the induction of hepatic gluconeogenesis. Triggering of the cAMP pathway in hepatocytes stimulates the gluconeogenic program via the PKA-mediated phosphorylation of CREB and dephosphorylation of the cAMP regulated CREB coactivators CRTC2 and CRTC3. In parallel, decreases in circulating insulin also increase gluconeogenic gene expression via the de-phosphorylation and activation of the forkhead transcription factor FOXO1. Hepatic gluconeogenesis is increased in insulin resistance where it contributes to the attendant hyperglycemia. Whether selective activation of the hepatic CREB/CRTC pathway is sufficient to trigger metabolic changes in other tissues is unclear, however. Modest hepatic expression of a phosphorylation-defective and therefore constitutively active CRTC2S171,275A protein increased gluconeogenic gene expression under fasting as well as feeding conditions. Circulating ...
Putative chromatin remodeling complexes, such as the yeast SWI/SNF complex (for a recent review see Burns and Peterson, 1997) and its mammalian homologs (Kwon et al., 1994; Wang et al., 1996), histone acetyltransferases, such as GCN5 (Brownell et al., 1996), CBP (Bannister and Kouzarides, 1996), P300 (Ogryzko et al., 1996), P/CAF (Yang et al., 1996) and TAFII250 (Mizzen et al., 1996), and histone deacetylases (Taunton et al., 1996; Alland et al., 1997; Hassig et al., 1997) are emerging as key players in the processes of cellular differentiation and oncogenesis. These factors appear to act as effectors for a large number of sequence‐specific transcription factors such as members of the nuclear receptor superfamily (Hanstein et al., 1996, Kamei et al., 1996; Heinzel et al., 1997; Nagy et al., 1997), the myb proto‐oncogene (Dai et al., 1996), cAMP response element binding protein (Kwok et al., 1994), the proto‐oncogenes c‐jun and c‐fos (Bannister and Kouzarides, 1995; Kamei et al., 1996), ...
We have demonstrated in the present study that the regulatory subunit RIIβ of PKA can interact with a CRE both physically and functionally. Our results showing that the RIIβ is a CRE transcription factor raises the possibility for an entirely different mechanism for cAMP regulation of gene expression. An established mechanism of cAMP/CRE-regulated transcription involves the CREB/ATF family of transcription factors. However, the CREB/ATF transcription factor family is unlikely to mediate all transcriptional responses to cAMP during development, differentiation, and endocrine homeostasis. The recent demonstration of CREB/ATF-independent cAMP gene regulation through orphan nuclear hormone receptor confirms the presence of multiple mechanisms for cAMP-regulated transcription (24, 25).. The function of R subunit in PKA has been confined to its inhibition of the C subunit. Recently, however, binding of R subunit to proteins other than the C subunit of PKA has been explored with the yeast two-hybrid ...
A 58-amino acid region mediates the core transactivation activity of the glucocorticoid receptor tau 1 activation domain. This tau 1 core domain is unstructured in aqueous buffers, but in the presence of trifluoroethanol three Alpha-helical segments are induced. Two of these putative structural modules have been tested in different combinations with regard to transactivation potential in vivo and binding capacity to the coactivators in vitro, The results show that whereas single modules are not transcriptionally active, any combination of two or three modules is sufficient, with trimodular constructs having the highest activity. However, proteins containing one, two, or three segments bind Ada2 and cAMP-response element-binding protein with similar affinity. A single segment is thus able to bind a target factor but cannot transactivate target genes significantly. The results are consistent with models in which activation domains are comprised of short activation modules that allow multiple ...
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Fibronectin (FN) is transactivated by human papillomavirus type 16 (HPV16) E6 via the induction of c-Jun-ATF-2 complexes binding to the cyclic AMP response element (CRE) in the FN promoter. The present study analyzed c-Jun regulation of FN gene expression. Northern and immunoblot analyses showed that c-Jun expression was enhanced in HPV16-E6-expressing cells. However, mouse 10T1/2 cell lines overexpressing c-Jun showed an inverse correlation between the expression levels of c-Jun and those of FN. Luciferase assays indicated that the FN promoter was strongly repressed in c-Jun-overexpressing mouse 10T1/2 cells. Deletion and mutation analyses of the FN promoter revealed that repression of the FN promoter by c-Jun depends on the CRE located at -160 relative to the start site of transcription. Supershift assays of CRE-bound complexes from HPV16-E6-expressing and c-Jun-overexpressing cells suggested that the presence of ATF-2 in the complexes binding to CRE was required for the transactivation of the ...
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Rasd1 is becoming somewhat of avant-garde member of the Ras family of GTPases by performing many non-conventional signaling functions. Our identification of Rasd1 in Avp neurons starts a new chapter for this small GTPase. Here we show that Rasd1 is rapidly induced by stress in the PVN, and by elevated plasma osmolality in the PVN and SON of the hypothalamus. We propose that the abundance of RASD1 in MCN and PCNs, based on its inhibitory actions on CREB phosphorylation, is an important mechanism for controlling the transcriptional responses to stressors in both the PVN and SON. In MCN we show, by virally mediated overexpression of Rasd1, that Rasd1 inhibits HS induced stimulation of cAMP inducible genes. When a CAAX box deficient mutant form of Rasd1 is expressed in the SON cAMP inducible genes were further increased by SL. These effects likely occur through modulation of cAMP-PKA-CREB signaling pathway.. Our interest in Rasd1 began following identification increased expression of this gene in ...
Due to all the patients scoring CREB3L1+ with degrees of PTN expression in the study cohort, the survival rates for the patients were compared based on the separate changes in the CREB3L1 and PTN expression levels. Our results revealed that percentages of survivors with CREB3L1− and/or PNT+ started to drop at the seventh month. With expanding time, the number of the survivors in the patients with CREB3L1− and PTN+ reached ~35 and 32% with 30 and 33% decreases as compared with the CREB3L1+ and PTN− survivors at the time point of 30 h, respectively. These findings indicated that absence of CREB3L1 and presence of PTN expression in the tumor cells obviously shortened the length of survival time. In statistical analysis, there was negative or positive correlation between the changes in CREB3L1 and PTN expression and the numbers of the survivors. The results supported the consideration that a combining measure of both protein expressions was more conclusive than a single measure of the proteins ...
Read "Complete nucleotide sequence, genomic organization, and promoter analysis of the murine survival motor neuron gene (Smn), Mammalian Genome" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Looking for Negative regulatory element-binding protein? Find out information about Negative regulatory element-binding protein. Christianity the second person of the Trinity, Jesus Christ one of the earliest Lower Paleolithic cultures in northwestern India and in Pakistan. Explanation of Negative regulatory element-binding protein
The serotonergic system plays a significant regulatory role in osteoblast differentiation and proliferation. Serotonin (five-hydroxytryptamine or 5HT) may promote or inhibit osteoblast proliferation depending upon the serotonin receptor isoforms expressed by the cell. Classically, 5HT receptor 1B (5HTR1B) reduces osteoblast proliferation by inhibiting phosphorylation of the cAMP response element binding protein (CREB) transcription factor. Conversely, 5HT receptor 2A (5HTR2A) activation positively influences osteoblast proliferation through extracellular signal-regulated kinase (ERK) signaling. Despite the serotonergic system being well characterized in normal osteoblasts, minimal information concerning the influence of serotonin on malignant osteoblasts is reported. The objectives of our study were to elucidate the expression, function, and potential of therapeutic targeting of these two receptors in a canine osteosarcoma cell line (COS) and primary normal canine osteoblasts (CnOb). Equal ...
GO Terms Descrition:, locomotor rhythm, positive regulation of transcription from RNA polymerase II promoter, R3/R4 cell fate commitment, synapse assembly, compound eye development, hemopoiesis, transcription factor binding, compound eye morphogenesis, protein complex, protein binding, smoothened signaling pathway, neurogenesis, Wnt signaling pathway, glial cell migration, zinc ion binding, nucleus, neurotransmitter secretion, transcription cofactor activity, histone acetyltransferase activity, regulation of transcription, DNA-templated, histone H4-K12 acetylation, histone H4-K8 acetylation, histone H3-K27 acetylation, histone H3-K18 acetylation, histone acetyltransferase activity (H3-K18 specific), histone acetyltransferase activity (H3-K27 specific), cAMP response element binding protein binding, circadian regulation of gene expression, DNA replication checkpoint, R7 cell differentiation, transcription coactivator activity, regulation of mitosis ...
Request for :- spinal muscular atrophy free sample page. Spinal Muscular Atrophy (SMA) Epidemiology The Spinal Muscular Atrophy (SMA) epidemiology division provide insights about historical and current Spinal Muscular Atrophy (SMA) patient pool and forecasted trend for every seven major countries. It helps to recognize the causes of current and forecasted trends by exploring numerous studies and views of key opinion leaders. This part of the DelveInsight report also provides the diagnosed patient pool and their trends along with assumptions undertaken. Spinal Muscular Atrophy (SMA) Market Outlook. The Spinal Muscular Atrophy (SMA) market outlook of the report helps to build the detailed comprehension of the historic, current, and forecasted Spinal Muscular Atrophy (SMA) market trends by analyzing the impact of current therapies on the market, unmet needs, drivers and barriers and demand of better technology. This segment gives a thorough detail of Spinal Muscular Atrophy (SMA) market trend of ...

Ras Activity Oscillates in the Mouse Suprachiasmatic Nucleus and Modulates Circadian Clock Dynamics - PubMedRas Activity Oscillates in the Mouse Suprachiasmatic Nucleus and Modulates Circadian Clock Dynamics - PubMed

The activation of the extracellular signal-regulated kinases 1 and 2 (ERK1,2) and cAMP response element-binding protein (CREB)- ... Cyclic AMP Response Element-Binding Protein / metabolism Actions. * Search in PubMed * Search in MeSH ... The activation of the extracellular signal-regulated kinases 1 and 2 (ERK1,2) and cAMP response element-binding protein (CREB)- ... Protein Kinases in the Photic Signaling of the Mammalian Circadian Clock. Alessandro MS, Golombek DA, Chiesa JJ. Alessandro MS ...
more infohttps://pubmed.ncbi.nlm.nih.gov/25762011/

Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human...Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human...

Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human ... Cyclic AMP response element-binding protein H (CREBH) was identified as a liver-specific bZIP transcription factor [10, 11]. ... The cyclic AMP response element-binding protein H (CREBH) plays important roles in hepatic lipogenesis, fatty acid oxidation, ... J. H. Lee, P. Giannikopoulos, S. A. Duncan et al., "The transcription factor cyclic AMP-responsive element-binding protein H ...
more infohttps://www.hindawi.com/journals/bmri/2013/892491/

Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human...Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human...

Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human ... J. H. Lee, P. Giannikopoulos, S. A. Duncan et al., "The transcription factor cyclic AMP-responsive element-binding protein H ... acute phase response by regulating expression of cyclic adenosine monophosphate responsive element binding protein H," ... cAMP responsive element-binding protein H)," Journal of Biological Chemistry, vol. 286, no. 49, pp. 41972-41984, 2011. View at ...
more infohttps://www.hindawi.com/journals/bmri/2013/892491/ref/

Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human...Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human...

Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ...
more infohttps://jvi.asm.org/content/75/4/1842?ijkey=b70e35753a95ccf8a7ccfbee49b930b0e54eba6c&keytype2=tf_ipsecsha

Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene...Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene...

Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene ... Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene ... Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene ... Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene ...
more infohttp://molpharm.aspetjournals.org/content/47/6/1112?ijkey=f57d00757d2925a7aa5b7959df46805804181fdf&keytype2=tf_ipsecsha

Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta...Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta...

Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ...
more infohttp://molpharm.aspetjournals.org/content/47/3/439

Mitochondrial cyclic AMP response element-binding protein (CREB) mediates mitochondrial gene expression and neuronal survival. ...Mitochondrial cyclic AMP response element-binding protein (CREB) mediates mitochondrial gene expression and neuronal survival. ...

Here we report that CREB is present in the mitochondrial matrix of neurons and that it binds directly to cyclic AMP response ... Cyclic AMP response element-binding protein (CREB) is a widely expressed transcription factor whose role in neuronal protection ... Cyclic AMP response element-binding protein (CREB) is a widely expressed transcription factor whose role in neuronal protection ... Mitochondrial cyclic AMP response element-binding protein (CREB) mediates mitochondrial gene expression and neuronal survival. ...
more infohttps://www.semanticscholar.org/paper/Mitochondrial-cyclic-AMP-response-element-binding-Lee-Kim/33f24fbed87b7159e66af9edc651c7da51fd52f8

Cyclic AMP Response Element-Binding Protein | The Chopra LibraryCyclic AMP Response Element-Binding Protein | The Chopra Library

Shift from extracellular signal-regulated kinase to AKT/cAMP response element-binding protein pathway increases survival-motor- ... the neurogenic potential of EGb 761 and its effect on cAMP response element binding protein (CREB) were examined in a double ... The transcription factor cAMP responsive element-binding protein 1 (CREB1) has a complex influence on behavioural responses to ... CREB1 and CREB-binding protein in striatal medium spiny neurons regulate behavioural responses to psychostimulants. ...
more infohttp://isharonline.org/tags/cyclic-amp-response-element-binding-protein

Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit - Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA...Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit - Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA...

Cyclic AMP Response Element Binding Protein) ELISA Kit based in Delhi, India ... Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit ... Rat CHD3(Chromodomain Helicase DNA Binding Protein 3) ELISA Kit. *Rat CHD5(Chromodomain Helicase DNA Binding Protein 5) ELISA ... Rat MBP-C/MBL2(Mannma Binding Protein C/Mannan Binding Lectin 2) ELISA Kit ...
more infohttp://www.srgroupchemical.com/rat-creb-cyclic-amp-response-element-binding-protein-elisa-kit-4472778.html

Role of cyclic AMP response element-binding protein in insulin-like growth factor-I receptor up-regulation by sex steroids in...Role of cyclic AMP response element-binding protein in insulin-like growth factor-I receptor up-regulation by sex steroids in...

Role of cyclic AMP response element-binding protein in insulin-like growth factor-I receptor up-regulation by sex steroids in ... Role of cyclic AMP response element-binding protein in insulin-like growth factor-I receptor up-regulation by sex steroids in ... Role of cyclic AMP response element-binding protein in insulin-like growth factor-I receptor up-regulation by sex steroids in ... stim ulation with either androgens or estrogens up-regulates IGF-IR by inducing cyclic AMP response element-binding protein ( ...
more infohttps://www.iris.unict.it/handle/20.500.11769/318458

Frontiers | Coordinating Gene Expression and Axon Assembly to Control Axon Growth: Potential Role of GSK3 Signaling | Frontiers...Frontiers | Coordinating Gene Expression and Axon Assembly to Control Axon Growth: Potential Role of GSK3 Signaling | Frontiers...

Cyclic AMP Response Element-Binding Protein. Role of CREB in Axon Growth and Regeneration. During development, several ... axon growth by regulation of gene expression via the transcription factor cyclic AMP response element-binding protein (CREB; ... cAMP response element binding protein CREB by cAMP-dependent protein kinase A and glycogen synthase kinase-3 alters DNA-binding ... and stress-activated protein kinase 1 mediates cAMP response element-binding protein phosphorylation and activation by ...
more infohttps://www.frontiersin.org/articles/10.3389/fnmol.2012.00003/full

finals - gene expression Flashcards by Estee C | Brainscapefinals - gene expression Flashcards by Estee C | Brainscape

cyclic AMP response element binding protein 73 ____ the co activator of CREB ... an example of a response element that binds to heat-specific transcription factors that are produced by elevated temperatures ... d. one transcription activator can bind to the same response element in multiple genes that each have unique promoters and ... a. consists of a single protein with two similar domains. b. Binding of TBP distorts DNA at TATA box. c. binding creates an 80 ...
more infohttps://www.brainscape.com/flashcards/finals-gene-expression-4548148/packs/6223664

The quantum physiology of oxygen; from electrons to the evolution of redox signaling in the human brain | SpringerLinkThe quantum physiology of oxygen; from electrons to the evolution of redox signaling in the human brain | SpringerLink

FIH, factor inhibiting HIF; CBP, cyclic AMP-response element binding protein; HRE, hypoxia response element ... However, in hypoxia, HIF-1α hydroxylation does not occur, stabilizing HIF-1 where it binds to a hypoxia response element ... As an element, oxygen (O) is unique; it is the third most abundant element in the universe after hydrogen and helium, the ... 4a). HIF-1 consists of an oxygen-sensitive HIF-1 alpha (α) subunit that heterodimerizes with the HIF-1beta (β) subunit to bind ...
more infohttps://link.springer.com/article/10.1186%2Fs42234-018-0014-7

Current Issue : Anti-Cancer DrugsCurrent Issue : Anti-Cancer Drugs

Discovery of selective inhibitors for cyclic AMP response element-binding protein: a combined ligand and structure-based ... ABT-737 and erufosine combination against castration-resistant prostate cancer: a promising but cell-type specific response ...
more infohttps://journals.lww.com/anti-cancerdrugs/pages/currenttoc.aspx

GPER | Cancer Genetics WebGPER | Cancer Genetics Web

Cyclic AMP Response Element-Binding Protein. *Culture Media, Conditioned. *Insulin-Like Growth Factor Binding Protein 1 ... G-protein coupled receptor activity - G-protein coupled receptor signaling pathway - Golgi membrane - hormone binding - ... The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5- ... Screening and Identification of a Phage Display Derived Peptide That Specifically Binds to the CD44 Protein Region Encoded by ...
more infohttp://www.cancerindex.org/geneweb/GPER.htm

Genome-wide transcriptional profiling linked to social class in asthma | ThoraxGenome-wide transcriptional profiling linked to social class in asthma | Thorax

AP1, activator protein 1; CREB, cyclic AMP response element binding protein; NFκB, nuclear factor κB; NF-Y, nuclear factor Y.. ... Bioinformatic analysis suggested that decreased activity of cyclic AMP response element binding protein and nuclear factor Y ... Cyclic AMP response element binding protein (CREB) TFBMs were significantly less prevalent in promoters of genes overexpressed ... Regulation of asthma-related T-cell cytokines by the cyclic AMP-dependent signalling pathway. Clin Exp Allergy 2000;30:920-6. ...
more infohttps://thorax.bmj.com/content/64/1/38?ijkey=80ef4692de676d47c5f9aa5e843b78bf2c752598&keytype2=tf_ipsecsha

Investigation of the role of SMN1 and SMN2 haploinsufficiency as a risk factor for Hirayamas disease: clinical,...Investigation of the role of SMN1 and SMN2 haploinsufficiency as a risk factor for Hirayama's disease: clinical,...

0/Cyclic AMP Response Element-Binding Protein; 0/Nerve Tissue Proteins; 0/RNA-Binding Proteins; 0/SMN Complex Proteins; 0/SMN1 ... Cyclic AMP Response Element-Binding Protein / genetics*. Electromyography. Female. Forearm / innervation*. Genetic ... protein, human; 0/SMN2 protein, human; 0/Survival of Motor Neuron 1 Protein; 0/Survival of Motor Neuron 2 Protein ... RNA-Binding Proteins / genetics*. SMN Complex Proteins. Spain. Spinal Cord / pathology. Survival of Motor Neuron 1 Protein. ...
more infohttp://www.biomedsearch.com/nih/Investigation-role-SMN1-SMN2-haploinsufficiency/17850955.html

Transplantation of Human Pericyte Progenitor Cells Improves the Repair of Infarcted Heart Through Activation of an Angiogenic...Transplantation of Human Pericyte Progenitor Cells Improves the Repair of Infarcted Heart Through Activation of an Angiogenic...

cyclic AMP response element-binding protein. EC. endothelial cell. Erk. extracellular signal regulated kinase. HUVEC. human ... together with its transcriptional activator cyclic AMP response element-binding protein, on stimulation by H/S or vascular ... expression of target genes by inducing the phosphorylation of the transcription factor cAMP response element binding protein ( ... methyl CpG binding protein 2. MI. myocardial infarction. miR-132. microRNA-132. MNC. mononuclear cells. MSC. mesenchymal stem ...
more infohttp://circres.ahajournals.org/content/109/8/894

Eco-friendly disposal of expired anti-tuberculosis drug Isoniazid and its role in the protection of metalEco-friendly disposal of expired anti-tuberculosis drug Isoniazid and its role in the protection of metal

Cyclic AMP response element-binding protein prevents endothelial permeability.... Source: Frontiers in Pharmacology - April 24 ... Gene Ontology Analysis for Drug Targets of the Whole Genome Transcriptome of Human Vascular Endothelial Cells in Response to ...
more infohttps://medworm.com/681433065/eco-friendly-disposal-of-expired-anti-tuberculosis-drug-isoniazid-and-its-role-in-the-protection-of-/

Developmental regulation of neuronal survival by adenosine in the in vitro and in vivo avian retina depends on a shift of...Developmental regulation of neuronal survival by adenosine in the in vitro and in vivo avian retina depends on a shift of...

Regarding cyclic nucleotide responsive element binding protein (CREB) phosphorylation, cultures from E6 embryos behave in an ... DPMA-induced cell death involved activation of A2a receptors and the phospholipase C/protein kinase C but not the cAMP/protein ... CREB dephosphorylation induced by DPMA in E6C0 cultures was dependent on the Phospholipase C/protein kinase C pathway. ... Previous studies have shown a cAMP/protein kinase A-dependent neuroprotective effect of adenosine on glutamate or re-feeding- ...
more infohttps://www.semanticscholar.org/paper/Developmental-regulation-of-neuronal-survival-by-in-Socodato-Brito/0221074ab95e18fb3ea11394109491680a02efb9

Cassiae semen: A review of its phytochemistry and pharmacology (Review)Cassiae semen: A review of its phytochemistry and pharmacology (Review)

... phosphorylated cyclic AMP response element binding protein; BDNF, brain-derived neurotrophic factor; DA, dopaminergic; MDA, ... properties and the induced upregulated expression of phosphorylated cyclic AMP response element binding protein and brain- ... sterol regulatory element-binding protein-1c, hormone-sensitive lipase and triacylglycerol hydrolase, however, tumor necrosis ... sterol regulatory element-binding protein-1c; HSL, hormone-sensitive lipase; TGH, triacylglycerol hydrolase; FAS, tumor ...
more infohttps://www.spandidos-publications.com/10.3892/mmr.2017.6880

Antibodies Search | ALZFORUMAntibodies Search | ALZFORUM

CREB (cyclic-AMP response element binding protein) phosphorylated and non-phosphorylated forms.. -. ... detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP). -. CREB binding protein. Acris Antibodies ... detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP). -. CREB binding protein. Acris Antibodies ... detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP). -. CREB binding protein. Acris Antibodies ...
more infohttps://www.alzforum.org/antibodies/search?category%5B645%5D=CREB,%20cAMP,%20CBP

Progress Report 2010: Memory and ForgettingProgress Report 2010: Memory and Forgetting

One example is cyclic-AMP response element-binding protein, or CREB. In both fruit flies and sea slugs, early studies ... which help rewire the brain in response to visual input. Evolutionary biology shows that Otx2 developed from a protein in fruit ... to constantly overexpress a protein called alpha-CaM kinase II. But the proteins activity was controlled by carefully timed ... One recent example of progress in this area is Joe Tsiens work with the protein alpha-CaM kinase II in mice.5 Having shown ...
more infohttp://www.dana.org/Publications/ReportDetails.aspx?id=44351

ESAT-6 Inhibits Production of IFN-γ by Mycobacterium tuberculosis-Responsive Human T Cells | The Journal of ImmunologyESAT-6 Inhibits Production of IFN-γ by Mycobacterium tuberculosis-Responsive Human T Cells | The Journal of Immunology

Cyclic AMP response element-binding protein positively regulates production of IFN-γ by T cells in response to a microbial ... cyclic AMP response element binding protein (CREB), activating transcription factor (ATF)-2 and c-Jun bind to the IFN-γ ... culture filtrate protein of 10 kDa; CREB, cyclic AMP response element binding protein; RD, region of difference. ... Reduced expression of nuclear cyclic adenosine 5′-monophosphate response element-binding proteins and IFN-γ promoter function ...
more infohttp://www.jimmunol.org/content/182/6/3668.long

Frontiers | Disruption of Maternal Parenting Circuitry by Addictive Process: Rewiring of Reward and Stress Systems | PsychiatryFrontiers | Disruption of Maternal Parenting Circuitry by Addictive Process: Rewiring of Reward and Stress Systems | Psychiatry

Jin, S. H., Blendy, J. A., and Thomas, S. A. (2005). Cyclic AMP response element-binding protein is required for normal ... Kreibich, A. S., and Blendy, J. A. (2004). cAMP response element-binding protein is required for stress but not cocaine-induced ... Altered sensitivity to rewarding and aversive drugs in mice with inducible disruption of cAMP response element-binding protein ... Gammie, S. C., Seasholtz, A. F., and Stevenson, S. A. (2008b). Deletion of corticotropin-releasing factor binding protein ...
more infohttps://www.frontiersin.org/articles/10.3389/fpsyt.2011.00037/full
  • Conversely, downregulation of Ras activity by blocking its function with an antibody in oscillating cell cultures reduced protein levels and increased phosphorylation of GSK3β and lengthened the period of BMAL1 promoter-driven luciferase activity. (nih.gov)
  • C/EBP proteins are all members of the b-ZIP family of transcription factors and share a highly homologous carboxy terminus that contains the basic and leucine zipper protein domains. (asm.org)
  • While C/EBP proteins are expressed in many human tissues, high levels of C/EBP mRNA and protein expression are limited to only a few cell types, including cells of the myeloid lineage. (asm.org)
  • The different C/EBP family members homo- and heterodimerize through their leucine zipper regions and bind to their cognate DNA sequences through the corresponding basic regions. (asm.org)
  • In response to drug exposure, CREB1 is phosphorylated in the striatum, a structure that is critically involved in reward-related learning. (isharonline.org)
  • We conclude that ESAT-6 directly inhibits human T cell responses to mycobacterial Ags by affecting TCR signaling pathways downstream of ZAP70. (jimmunol.org)
  • Vaccine development hinges on understanding the molecular mechanisms of the host-pathogen interaction, particularly those that affect T cell responses, because this is believed to be pivotal for controlling M. tuberculosis infection. (jimmunol.org)
  • It has a modular organization with several protein-protein interaction domains (i.e. (jneurosci.org)
  • Axon growth requires the coordinated regulation of gene expression in the neuronal soma, local protein translation in the axon, anterograde transport of synthesized raw materials along the axon, and assembly of cytoskeleton and membranes in the nerve growth cone. (frontiersin.org)
  • The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. (cancerindex.org)
  • By the mid-1990s, studies had already suggested a basic difference in the molecular mechanisms of short-term versus long-term memories: The latter required new protein synthesis, while the former did not. (dana.org)
  • For instance, lysine 221 acetylation facilitates RELA dissociation from IκBα and enhances its DNA-binding affinity. (wikipedia.org)
  • SVPs secrete vascular endothelial growth factor A, angiopoietin-1, and chemokines and induce an endogenous angiocrine response by the host, through recruitment of vascular endothelial growth factor B expressing monocytes. (ahajournals.org)
  • Previous studies reported that CCAAT/enhancer binding protein β (C/EBP β) can transactivate the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in transient transfection analyses and that the LTR contains three binding sites for this protein ( 39 ). (asm.org)
  • to a region near the N-terminus (aa 5-45) of the human protein conj. (alzforum.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Adenovirus-mediated transduction of 15-lipoxygenase 2 (15-LOX2) caused increased production of 15-HETE in VSMCs and enhanced IL-6 expression, SMC migration from media to intimal region, and neointima formation in response to arterial injury. (ahajournals.org)