A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
A basic leucine zipper transcription factor that is highly homologous to ACTIVATING TRANSCRIPTION FACTOR 2. It binds the consensus site TGACGTCA of the cyclic AMP response element in partnership with either PROTO-ONCOGENE PROTEINS C-JUN or activating transcription factor 2.
A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
A publication issued at stated, more or less regular, intervals.
Individual's rights to obtain and use information collected or generated by others.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
A class of ionotropic glutamate receptors characterized by affinity for N-methyl-D-aspartate. NMDA receptors have an allosteric binding site for glycine which must be occupied for the channel to open efficiently and a site within the channel itself to which magnesium ions bind in a voltage-dependent manner. The positive voltage dependence of channel conductance and the high permeability of the conducting channel to calcium ions (as well as to monovalent cations) are important in excitotoxicity and neuronal plasticity.
A potent noncompetitive antagonist of the NMDA receptor (RECEPTORS, N-METHYL-D-ASPARTATE) used mainly as a research tool. The drug has been considered for the wide variety of neurodegenerative conditions or disorders in which NMDA receptors may play an important role. Its use has been primarily limited to animal and tissue experiments because of its psychotropic effects.
Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
An amino acid that, as the D-isomer, is the defining agonist for the NMDA receptor subtype of glutamate receptors (RECEPTORS, NMDA).
A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.
Drugs that bind to but do not activate excitatory amino acid receptors, thereby blocking the actions of agonists.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
A moderate-growing, photochromogenic species found in aquariums, diseased fish, and swimming pools. It is the cause of cutaneous lesions and granulomas (swimming pool granuloma) in humans. (Dorland, 28th ed)
Any of the infectious diseases of man and other animals caused by species of MYCOBACTERIUM.
A genus of gram-positive, aerobic bacteria. Most species are free-living in soil and water, but the major habitat for some is the diseased tissue of warm-blooded hosts.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
Substances elaborated by bacteria that have antigenic activity.
Continuous involuntary sustained muscle contraction which is often a manifestation of BASAL GANGLIA DISEASES. When an affected muscle is passively stretched, the degree of resistance remains constant regardless of the rate at which the muscle is stretched. This feature helps to distinguish rigidity from MUSCLE SPASTICITY. (From Adams et al., Principles of Neurology, 6th ed, p73)
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.

ATF-2-binding regulatory element is responsible for the Ly49A expression in murine T lymphoid line, EL-4. (1/3445)

To understand the mechanism of Ly49A-expression and its significance in T-cell differentiation, we analyzed the 5'-flanking region of the Ly49A gene in a search for the Ly49A-regulatory element. Since very few known regulatory elements have been found in this region, presumably a novel regulatory sequence(s) could exist. Accordingly, we defined the 13-bp regulatory element, 5'-ATGACGAGGAGGA-3', restricted to Ly49A-expression in EL-4 cells in comparison with two other representative cell lines tested. This element, designated as EL13, proved to be previously undiscovered by homology search and is highly homologous with several virus DNAs. Using EL13 as a probe we have cloned a cDNA encoding a binding protein to EL13. Its deduced nucleotide sequence revealed that EL13-binding protein is almost identical with rat ATF-2. Although ATF-2 is known to bind to cyclic AMP responsive element (CRE), EL13 shares five out of eight nucleotides with this consensus sequence. Our results suggested that ATF-2 may play an important role via binding to EL13 for the expression of Ly49A. These data will provide useful information for understanding T-cell and NK-cell differentiation in murine immune system.  (+info)

A critical role for cAMP response element-binding protein (CREB) as a Co-activator in sterol-regulated transcription of 3-hydroxy-3-methylglutaryl coenzyme A synthase promoter. (2/3445)

3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, a key regulatory enzyme in the pathway for endogenous cholesterol synthesis, is a target for negative feedback regulation by cholesterol. When cellular sterol levels are low, the sterol regulatory element-binding proteins (SREBPs) are released from the endoplasmic reticulum membrane, allowing them to translocate to the nucleus and activate SREBP target genes. However, in all SREBP-regulated promoters studied to date, additional co-regulatory transcription factors are required for sterol-regulated activation of transcription. We have previously shown that, in addition to SREBPs, NF-Y/CBF is required for sterol-regulated transcription of HMG-CoA synthase. This heterotrimeric transcription factor has recently been shown to function as a co-regulator in several other SREBP-regulated promoters, as well. In addition to cis-acting sites for both SREBP and NF-Y/CBF, the sterol regulatory region of the synthase promoter also contains a consensus cAMP response element (CRE), an element that binds members of the CREB/ATF family of transcription factors. Here, we show that this consensus CRE is essential for sterol-regulated transcription of the synthase promoter. Using in vitro binding assays, we also demonstrate that CREB binds to this CRE, and mutations within the CRE that result in a loss of CREB binding also result in a loss of sterol-regulated transcription. We further show that efficient activation of the synthase promoter in Drosophila SL2 cells requires the simultaneous expression of all three factors: SREBPs, NF-Y/CBF, and CREB. To date this is the first promoter shown to require CREB for efficient sterol-regulated transcription, and to require two different co-regulatory factors in addition to SREBPs for maximal activation.  (+info)

CCAAT/enhancer-binding protein beta is an accessory factor for the glucocorticoid response from the cAMP response element in the rat phosphoenolpyruvate carboxykinase gene promoter. (3/3445)

The cyclic AMP response element (CRE) of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene promoter is required for a complete glucocorticoid response. Proteins known to bind the PEPCK CRE include the CRE-binding protein (CREB) and members of the CCAAT/enhancer-binding protein (C/EBP) family. We took two different approaches to determine which of these proteins provides the accessory factor activity for the glucocorticoid response from the PEPCK CRE. The first strategy involved replacing the CRE of the PEPCK promoter/chloramphenicol acetyltransferase reporter plasmid (pPL32) with a consensus C/EBP-binding sequence. This construct, termed pDeltaCREC/EBP, binds C/EBPalpha and beta but not CREB, yet it confers a nearly complete glucocorticoid response when transiently transfected into H4IIE rat hepatoma cells. These results suggest that one of the C/EBP family members may be the accessory factor. The second strategy involved co-transfecting H4IIE cells with a pPL32 mutant, in which the CRE was replaced with a GAL4-binding sequence (pDeltaCREGAL4), and various GAL4 DNA-binding domain (DBD) fusion protein expression vectors. Although chimeric proteins consisting of the GAL4 DBD fused to either CREB or C/EBPalpha are able to confer an increase in basal transcription, they do not facilitate the glucocorticoid response. In contrast, a fusion protein consisting of the GAL4 DBD and amino acids 1-118 of C/EBPbeta provides a significant glucocorticoid response. Additional GAL4 fusion studies were done to map the minimal domain of C/EBPbeta needed for accessory factor activity to the glucocorticoid response. Chimeric proteins containing amino acid regions 1-84, 52-118, or 85-118 of C/EBPbeta fused to the GAL4 DBD do not mediate a glucocorticoid response. We conclude that the amino terminus of C/EBPbeta contains a multicomponent domain necessary to confer accessory factor activity to the glucocorticoid response from the CRE of the PEPCK gene promoter.  (+info)

CRE-mediated gene transcription in neocortical neuronal plasticity during the developmental critical period. (4/3445)

Neuronal activity-dependent processes are believed to mediate the formation of synaptic connections during neocortical development, but the underlying intracellular mechanisms are not known. In the visual system, altering the pattern of visually driven neuronal activity by monocular deprivation induces cortical synaptic rearrangement during a postnatal developmental window, the critical period. Here, using transgenic mice carrying a CRE-lacZ reporter, we demonstrate that a calcium- and cAMP-regulated signaling pathway is activated following monocular deprivation. We find that monocular deprivation leads to an induction of CRE-mediated lacZ expression in the visual cortex preceding the onset of physiologic plasticity, and this induction is dramatically downregulated following the end of the critical period. These results suggest that CRE-dependent coordinate regulation of a network of genes may control physiologic plasticity during postnatal neocortical development.  (+info)

Platelet-derived growth factor induces interleukin-6 transcription in osteoblasts through the activator protein-1 complex and activating transcription factor-2. (5/3445)

Platelet-derived growth factor (PDGF) BB, a mitogen that stimulates bone resorption, increases the expression of interleukin-6 (IL-6), a cytokine that induces osteoclast recruitment. The mechanisms involved in IL-6 induction by PDGF BB are poorly understood. We examined the effect of PDGF BB on IL-6 expression in cultures of osteoblasts from fetal rat calvariae (Ob cells). PDGF BB increased IL-6 mRNA and heterogeneous nuclear RNA levels, the rate of transcription, and the activity of base pairs (bp) -2906 to +20 IL-6 promoter fragments transiently transfected into Ob cells. Deletion analysis revealed two responsive regions, one containing an activator protein-1 (AP-1) site located between bp -276 and -257, and a second, less well defined, downstream of -257. Targeted mutations of a cyclic AMP-responsive element (CRE), and nuclear factor-IL-6 and nuclear factor-kappaB binding sites in a bp -257 to +20 IL-6 construct that was transfected into Ob cells, revealed that the CRE also contributed to IL-6 promoter induction by PDGF BB. Electrophoretic mobility shift assay revealed AP-1 and CRE nuclear protein complexes that were enhanced by PDGF BB. Supershift assays revealed binding of Jun and Fos to AP-1 and CRE sequences and binding of activating transcription factor-2 to CRE. In conclusion, PDGF BB induces IL-6 transcription in osteoblasts by regulating nuclear proteins of the AP-1 complex and activating transcription factor-2.  (+info)

pp60(v-src) induction of cyclin D1 requires collaborative interactions between the extracellular signal-regulated kinase, p38, and Jun kinase pathways. A role for cAMP response element-binding protein and activating transcription factor-2 in pp60(v-src) signaling in breast cancer cells. (6/3445)

The cyclin D1 gene is overexpressed in breast tumors and encodes a regulatory subunit of cyclin-dependent kinases that phosphorylate the retinoblastoma protein. pp60(c-src) activity is frequently increased in breast tumors; however, the mechanisms governing pp60(c-src) regulation of the cell cycle in breast epithelium are poorly understood. In these studies, pp60(v-src) induced cyclin D1 protein levels and promoter activity (48-fold) in MCF7 cells. Cyclin D1-associated kinase activity and protein levels were increased in mammary tumors from murine mammary tumor virus-pp60(c-src527F) transgenic mice. Optimal induction of cyclin D1 by pp60(v-src) involved the extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase members of the mitogen-activated protein kinase family. Cyclin D1 promoter activation by pp60(v-src) involved a cAMP response element-binding protein (CREB)/activating transcription factor 2 (ATF-2) binding site. Dominant negative mutants of CREB and ATF-2 but not c-Jun inhibited pp60(v-src) induction of cyclin D1. pp60(v-src) induction of CREB was blocked by the p38 inhibitor SB203580 or by mutation of CREB at Ser133. pp60(v-src) induction of ATF-2 was abolished by the c-Jun N-terminal kinase inhibitor JNK-interacting protein-1 or by mutation of ATF-2 at Thr69 and Thr71. CREB and ATF-2, which bind to a common pp60(v-src) response element, are transcriptionally activated by distinct mitogen-activated protein kinases. Induction of cyclin D1 activity by pp60(v-src) may contribute to breast tumorigenesis through phosphorylation and inactivation of the retinoblastoma protein.  (+info)

CREB regulates MHC class II expression in a CIITA-dependent manner. (7/3445)

The X2 box of MHC class II promoters is homologous to TRE/CRE elements and is required for expression of MHC class II genes. The X2 box-specific DNA binding activity, X2BP, was purified to homogeneity, sequenced, and identified as CREB. Transient transactivation experiments showed that CREB can cooperate with CIITA to enhance activation of transcription from MHC class II promoters in a dose-dependent manner. Binding of CREB to the class II promoter in vivo was demonstrated by a chromatin immunoprecipitation assay. Additionally, ICER, a dominant inhibitor of CREB function, was found to repress class II expression. These results demonstrate that CREB binds to the X2 box in vivo and cooperates with CIITA to direct MHC class II expression.  (+info)

Interaction of Gli2 with CREB protein on DNA elements in the long terminal repeat of human T-cell leukemia virus type 1 is responsible for transcriptional activation by tax protein. (8/3445)

The long terminal repeat (LTR) of human T-cell leukemia virus type 1 (HTLV-1) has two distinct DNA elements, one copy of TRE2S and three copies of a 21-bp sequence that respond to the viral trans-activator protein, Tax. Either multiple copies of the 21-bp sequence or a combination of one copy each of TRE2S and 21-bp sequence is required for efficient trans activation by Tax. In the trans activation of multiple copies of 21-bp sequence, CREB/ATF protein plays an essential role in forming a complex with Tax. To understand the role of TRE2S in trans activation of one copy of 21-bp sequence, we examined protein binding to the DNA elements by DNA affinity precipitation assay including Gli2 protein binding to TRE2S and CREB protein binding to 21-bp sequence. Binding of CREB to a DNA probe containing both elements, TRE2S-21bp probe, was dependent on Gli2 protein under restricted conditions and was enhanced in a dose-dependent fashion by the binding of Gli2 protein to the same probe. Mutation in either element abolished the efficient binding of CREB. A glutathione S-transferase fusion protein of a fragment of Gli2 was able to bind to CREB. Therefore, Gli2-CREB interaction on the DNA probe is proposed to stabilize CREB binding to DNA. Tax can bind to CREB protein on the DNA; therefore, stabilization of DNA binding of CREB results in more recruitment of Tax onto DNA. Conversely, Tax increased the DNA binding of CREB, although it had almost no effect on the binding of Gli2. These results suggest that Gli2 binds to the DNA element and interacts with CREB, resulting in more recruitment of Tax, which in turn stabilizes DNA binding of CREB. Similar cooperation of the protein binding to TRE2S-21bp probe was also observed in nuclear extract of an HTLV-1-infected T-cell line. Consistent with the Gli2-CREB interaction on the DNA elements, Tax-mediated trans activation was dependent on the size of the spacer between TRE2S and 21-bp sequence. The effective sizes of the spacer suggest that TRE2S in the LTR would cooperate with the second and third copies of the 21-bp sequence and contribute to trans activation of the viral gene transcription.  (+info)

The mouse CREB (cAMP responsive element binding protein) gene: structure, promoter analysis, and chromosomal localization, Co-Author, (1992) Genomics 13 (4), 974-982 ...
Fingerprint Dive into the research topics of The function of cyclic-adenosine monophosphate responsive element-binding protein in hematologic malignancies. Together they form a unique fingerprint. ...
Fragile X syndrome is caused by lack of fragile X mental retardation protein (FMRP) due to silencing of the FMR1 gene. The metabotropic glutamate receptors (mGluRs) in the central nervous system contribute to higher brain functions including learning/memory, mental disorders and persistent pain. The transcription factor cyclic AMP-responsive element binding protein (CREB) is involved in important neuronal functions, such as synaptic plasticity and neuronal survival. Our recent study has shown that stimulation of Group I mGluRs upregulated FMRP and activated CREB in anterior cingulate cortex (ACC), a key region for brain cognitive and executive functions, suggesting that activation of Group I mGluRs may upregulate FMRP through CREB signaling pathway. In this study, we demonstrate that CREB contributes to the regulation of FMRP by Group I mGluRs. In ACC neurons of adult mice overexpressing dominant active CREB mutant, the upregulation of FMRP by stimulating Group I mGluR is enhanced compared to wild-type
Looking for online definition of cAMP-responsive element-binding protein 3-like protein 4 in the Medical Dictionary? cAMP-responsive element-binding protein 3-like protein 4 explanation free. What is cAMP-responsive element-binding protein 3-like protein 4? Meaning of cAMP-responsive element-binding protein 3-like protein 4 medical term. What does cAMP-responsive element-binding protein 3-like protein 4 mean?
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TY - JOUR. T1 - Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15. AU - Xie, Fuchun. AU - Fan, Qiuhua. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. PY - 2019/1/1. Y1 - 2019/1/1. N2 - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, we designed an ester prodrug of 666-15 through a long-range O,N-acyl transfer reaction for improved aqueous solubility. Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB.. AB - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, ...
ATM has been shown to have important roles in the transcriptional regulation of gene expression by phosphorylation of transcription factors, such as p53, nuclear factor κB, E2F, cyclic AMP-responsive element binding protein, and BRCA1, and in maintenance of the normal functions of IGF-IR and telomeres (3, 13, 14, 32). ATM-dependent gene expression has been systematically studied by comparing cell lines from normal individuals and AT patients, isogenic cell lines with restoration of ATM in AT cells or small interfering RNA knockdown of ATM in wild-type cells, and ATM+/+/ATM−/− mice (3, 13, 21-23). More than 300 genes have been reported to display ATM-dependent expression although few genes were commonly identified in two or more experiments. The microarray results presented here identified 160 genes or expressed sequence tags (∼1% of the total on the array) that were differentially expressed in normal and AT fibroblast lines under basal growth conditions, of which about half were ...
Lysosomal-associated protein transmembrane-4 beta (LAPTM4B) is a potential proto-oncogene, whose overexpression is involved in cancer occurrence and progression. Its transcript is up-regulated in various types of solid tumors including breast cancer. However, its transcriptional regulation mechanism is still unclear. To investigate the mechanism of transcriptional regulation of LAPTM4B in human breast cancer cells, a series of luciferase reporter constructs and construct with mutated binding site for cAMP responsive element binding protein-1 (CREB1) were generated by PCR amplification and transiently transfected into breast cancer cells to determine the transcriptional activities of different promoter regions. The + 10 similar to+ 292 promoter region was possessed the highest transcriptional activity. The ability of CREB1 to bind the LAPMT4B promoter was confirmed by electrophoretic mobility shift assay, super-shift and RNA interference experiments. Our study identified the core promoter region ...
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity).
TY - JOUR. T1 - Design, synthesis and biological evaluation of regioisomers of 666-15 as inhibitors of CREB-mediated gene transcription. AU - Xie, Fuchun. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. N1 - Funding Information: This work was made possible through financial supports provided by the United States National Institutes of Health ( R01GM087305 ) and OHSU Office of Technology Transfer and Business Development . PY - 2017/2/1. Y1 - 2017/2/1. N2 - cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the pathogenesis and maintenance of various types of human cancers. Identification of small molecule inhibitors of CREB-mediated gene transcription has been pursued as a novel strategy for developing cancer therapeutics. We recently discovered a potent and cell-permeable CREB inhibitor called 666-15. 666-15 is a bisnaphthamide and has been shown to possess efficacious anti-breast cancer activity without toxicity in vivo. In this study, we ...
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
TY - JOUR. T1 - Nuclear factor-kappaB and cAMP response element binding protein mediate opposite transcriptional effects on the Flk-1/KDR gene promoter.. AU - Illi, B.. AU - Puri, P.. AU - Morgante, L.. AU - Capogrossi, M. C.. AU - Gaetano, C.. PY - 2000/6/23. Y1 - 2000/6/23. N2 - -The vascular endothelial growth factor receptor Flk-1/KDR is highly expressed during development and almost disappears in adult tissues. Despite its biological relevance, little is known about the molecular mechanisms controlling its expression. In the present work, it is shown that cAMP response element binding protein (CREB) and nuclear factor-kappaB (NF-kappaB)-related antigens bind specific sequences in the Flk-1/KDR promoter. Functional studies demonstrate that cAMP represses whereas tumor necrosis factor-alpha, an activator of NF-kappaB, stimulates promoter activity. Histone acetyltransferases (HATs) P/CAF and CBP/p300 together with p65/RelA, the catalytic subunit of NF-kappaB, increase Flk-1/KDR promoter ...
The results reveal an essential survival pathway in malignant glioma, whereby activation of a RAS-mitogen-activated protein kinase or phosphoinositide-3-kinase signaling cascade leads to induction of the transcription factor cAMP response element-binding protein-3-like-2 (CREB3L2), which directly activates ATF5 expression. ATF5, in turn, promotes survival by stimulating transcription of myeloid cell leukemia sequence-1 (MCL1), an antiapoptotic B cell leukemia-2 family member. [Nat Med ...
Read A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor and associates with ABA signaling, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
TY - JOUR. T1 - Relaxin activates peroxisome proliferator-activated receptor γ(PPAR γ)through a pathway involving PPAR γ coactivator 1α (PGC1α). AU - Singh, Sudhir. AU - Simpson, Ronda L.. AU - Bennett, Robert G.. PY - 2015/1/9. Y1 - 2015/1/9. N2 - Relaxin activation of its receptor RXFP1 triggers multiple signaling pathways. Previously, we have shown that relaxin activates &gamma transcriptional activity in a ligand-independent manner, but the mechanism for this effect was unknown. In this study, we examined the signaling pathways of downstream of RXFP1 leading to γ activation. Using cells stably expressing RXFP1, we found that relaxin regulation of &gamma activity requires accumulation of cAMP and subsequent activation of cAMP-dependent protein kinase (PKA). The activated PKA subsequently phosphorylated cAMP response element-binding protein (CREB) at Ser-133 to activate it directly, as well as indirectly through mitogen activated protein kinase p38 MAPK. Activated CREB was required for ...
TransAM CREB and TransAM pCREB Kits are DNA-binding ELISAs that quanify the activated transcription factors using a method that is faster and more sensitive than gelshift, without radioactivity and gels.
Background: Lowered sensitivity to the effects of ethanol increases the risk of developing alcoholism. Inbred mouse strains have been useful for the study of the genetic basis of various drug addiction-related phenotypes. Inbred Long-Sleep (ILS) and Inbred Short-Sleep (ISS) mice differentially express a number of genes thought to be implicated in sensitivity to the effects of ethanol. Concomitantly, there is evidence for a mediating role of cAMP/PKA/CREB signalling in aspects of alcoholism modelled in animals. In this report, the extent to which CREB signalling impacts the differential expression of genes in ILS and ISS mouse cerebella is examined. Results: A training dataset for Machine Learning (ML) and Exploratory Data Analyses (EDA) was generated from promoter region sequences of a set of genes known to be targets of CREB transcription regulation and a set of genes whose transcription regulations are potentially CREB-independent. For each promoter sequence, a vector of size 132, with ...
cAMP response element (CRE)-binding protein-like-2 (CREBL2) was identified in a search to find genes in a commonly deleted region on chromosome 12p13 flanked by ETV6 and CDKN1B genes, frequently associated with hematopoietic malignancies, as well as breast, non-small-cell lung and ovarian cancers. CREBL2 shares a 41% identity with CRE-binding protein (CREB) over a 48-base long region which encodes the bZip domain of CREB. The bZip domain consists of about 30 amino acids rich in basic residues involved in DNA binding, followed by a leucine zipper motif involved in protein dimerization. This suggests that CREBL2 encodes a protein with DNA binding capabilities. The occurance of CREBL2 deletion in malignancy suggests that CREBL2 may act as a tumor suppressor gene.
Abuse of opiates, including morphine, induced remarkable synaptic adaptation in several brain regions including ventral tegmental area (VTA), which underlay the induction and maintenance of opioid dependence and addiction. Scaffolding protein postsynaptic density protein 95 (PSD-95) is critically involved in the glutamatergic synaptic maturation and plasticity in the central neurons. The present study revealed a significantly increased mRNA and protein expression of PSD-95 in the VTA of the rats conditioned with morphine. The further chromatin immunoprecipitation study found an increased histone H3 acetylation in the promoter region of Dlg4. An upregulation of expression of phosphorylated cAMP response element-binding protein (pCREB) and the occupancy of pCREB in the Dlg4 promoter region were shown in the VTA of the morphine-conditioned rats. Inhibition of pCREB activity significantly decreased the histone H3 acetylation in Dlg4 promoter region, PSD-95 upregulation, enhancement of glutamatergic strength
Fasting elicits transcriptional programs in hepatocytes leading to glucose and ketone production. This transcriptional program is regulated by many transcription factors (TFs). To understands how this complex network regulates the metabolic response to fasting we aimed at isolating the enhancers and TFs dictating it. Measuring chromatin accessibility revealed that fasting massively reorganizes liver chromatin, exposing numerous fasting-induced enhancers. By utilizing computational methods in combination with dissecting enhancers features and TF cistromes, we implicated four key TFs regulating the fasting response: glucocorticoid receptor (GR), cAMP responsive element binding protein 1 (CREB1), peroxisome proliferator activated receptor alpha (PPARA) and CCAAT/enhancer binding protein beta (CEBPB). These TFs regulate fuel production by two distinctly-operating modules, each controlling a separate metabolic pathway. The gluconeogenic module operates through assisted loading whereby GR doubles the ...
CREB3L1 antibody [C3], C-term (cAMP responsive element binding protein 3-like 1) for WB. Anti-CREB3L1 pAb (GTX104818) is tested in Human samples. 100% Ab-Assurance.
Early odor preference learning in rats is associated with increases of phosphorylated CREB (pCREB) in mitral cells of the olfactory bulb. In the present study, herpes simplex virus expressing CREB (HSV-CREB) and dominant-negative mutant CREB (HSV-mCREB) have been injected into the bulb to assess a causal role for CREB and pCREB in this model. Odor paired with stroking or with the β-adrenoceptor agonist isoproterenol produces odor approach 24 hr later. Isoproterenol-induced learning exhibits an inverted U curve dose-dependent learning relationship with both low and high doses failing to produce learning. pCREB increases have only been seen at the learning effective dose. In the present study, injection of an HSV vector expressing mutant CREB into the olfactory bulb prevented learning induced by stroking. Control HSV expressing LacZ was without effect. Expression of mutant CREB shifted the dose-learning curve for isoproterenol to the right such that a higher dose was required to induce learning. ...
Incubation of 3T3-L1 preadipocytes with isobutylmethylxanthine (IBMX), dexamethasone, and insulin, alone or in combination, demonstrated that IBMX, which increased cAMP-response element-binding protein (CREB) phosphorylation, was the predominant regulator of Pde3b expression. Real time PCR and immunoblotting indicated that in 3T3-L1 preadipocytes, IBMX-stimulated induction of Pde3b mRNA and protein was markedly inhibited by dominant-negative CREB proteins. By transfecting preadipocytes, differentiating preadipocytes, and HEK293A cells with luciferase reporter vectors containing different fragments of the 5- flanking region of the Pde3b gene, we identified a distal promoter that contained canonical cis-acting cAMP-response elements (CRE) and a proximal, GC-rich promoter region, which contained atypical CRE. Mutation of the CRE sequences dramatically reduced distal promoter activity; H89 inhibited IBMX-stimulated CREB phosphorylation and proximal and distal promoter activities. Distal promoter ...
The concentration of glucose in the bloodstream is regulated by glucose itself, along with the hormones insulin and glucagon. Glucagon stimulates gluconeogenesis in part by regulating phosphorylation of a transcriptional coactivator known as cyclic adenosine monophosphate response element-binding protein 2 (CRTC2). Dentin et al. (see the Perspective by Birnbaum) found that high concentrations of circulating glucose also regulate CRTC2, but do so through stimulation of the hexosamine biosynthetic pathway and consequent O-linked glycosylation of the same serine residue in CRTC2 that is modified by phosphorylation. Thus, CRTC2 integrates signals from hormones and nutrients and might be a target for efforts to treat abnormalities of glucose homeostasis that are associated with diabetes.. R. Dentin, S. Hedrick, J. Xie, J. Yates, III, M. Montminy, Hepatic glucose sensing via the CREB coactivator CRTC2. Science 319, 1402-1405 (2008). [Abstract] [Full Text]. M. J. Birnbaum, Sweet conundrum. Science 319, ...
4-PPBP is a molecule which binds to sigma receptors.[1] 4-PPBP decreases neuronal nitric oxide synthase (nNOS) activity and ischemia-evoked nitric oxide (NO) production. 4-PPBP provides neuroprotection; this involves the prevention of ischemia-induced intracellular Ca2+dysregulation.[2]4-PPBP protects neurons using a mechanism that activates the transcription factor cyclic adenosine monophosphate response element-binding protein (CREB). Neuroprotection that is associated with 4-PPBP increases Bcl-2 expression; Bcl-2 expression is regulated by CREB. [3] ...
Next, we focused on intermediate signaling molecules that are implicated in the mediation of cell motility and calcium signaling. Compared with control cells, MECs treated with recMFAP5 had higher expression of phosphorylated FAK (p-FAK) (Y861), p-PLC-γ1 (Y783), p-PKCθ (T538), p-ERK1/2 (T202/Y204), phosphorylated myosin regulatory light chain 2 (p-MLC2) (T18/S19), phosphorylated cyclic AMP-responsive element-binding protein (p-CREB) (S133), c-Jun, and p-c-Jun (S73), which may have led to the upregulation of LPP expression and thus increased cell motility and permeability (Figure 7G).. Because our data demonstrated that MFAP5-induced microvascular endothelial cell motility was suppressed in cells that had been pretreated with an anti-αVβ3 integrin antibody (Figure 7H) and that MFAP5-upregulated p-FAK (Y861) expression was suppressed in cells that had been pretreated with BAPTA-AM (1,2-bis-[2-aminophenoxy]-ethane-N,N,N′,N′-tetraacetic acid, tetraacetoxymethyl ester) (Supplemental Figure 9A ...
cAMP Response Element-Binding Protein Is Required for Stress But Not Cocaine-Induced Reinstatement is an eagle-i resource of type Journal article at The University of Pennsylvania.
Rabbit anti CREB1 antibody recognizes cyclic AMP-responsive element-binding protein 1 (CREB1). CREB1 is a transcription factor that has be
Defective heme synthesis in mammals has been suspected of causing neuropathy associated with porphyrias and lead poisoning. To determine the molecular action of heme in neuronal cells, we examined the effect of the inhibition of heme synthesis on nerve growth factor (NGF) signaling in PC12 cells. We found that the inhibition of heme synthesis by succinyl acetone interferes with NGF-induced neurite outgrowth in PC12 cells. Furthermore, we show that heme deficiency obliterates the activation of the signaling intermediates of the Ras-mitogen-activated protein kinase signaling pathway and its downstream target, the transcription activator cyclic AMP response element-binding protein. Strikingly, microarray expression analysis shows that the inhibition of heme synthesis selectively diminishes the induction of expression of a subset of neuron-specific genes by NGF, such as Ras and neurofilament proteins, whereas NGF induces the expression of several major classes of neuronal genes that encode ...
Our hypothesis is based on several lines of evidence. First, the effects of cAMP and DA on both 4xCRE (Figs. 3a,b, 5) and c-fos and BDNF mRNA expression (Fig. 6) are blocked by NMDAR antagonists. Second, two structurally distinct inhibitors of neuronal EAA uptake, TBOA (Fig. 7) and trans-PDC (supplemental Fig. S3, available at www.jneurosci.org as supplemental material), potentiated the stimulation of gene transcription by cAMP. Third, the aspartate+glutamate-, but not the glutamate-only-, scavenging system abolished stimulation of CREB-dependent gene transcription by forskolin (Fig. 8); the aspartate-scavenging enzyme, GOT, degrades l- but not d-aspartate demonstrating that l-aspartate is the active extracellular EAA in this signaling pathway. Finally, forskolin was found to induce release of aspartate but not glutamate (Fig. 9). Together, these results lead to the conclusion that cAMP-induced release of aspartate and the resulting activation of NR2B-containing NMDARs mediate the effects of ...
In type 2 diabetes, chronic hyperglycemia is detrimental to beta-cells, causing apoptosis and impaired insulin secretion. The transcription factor cAMP-responsive element-binding protein (CREB) is crucial for beta-cell survival and function. We inves
CG-001 is a selective Wnt/β-catenin signalling inhibitor with an IC50 of 3μM. ICG 001, a small molecule that down-regulates beta-catenin/T cell factor signaling by specifically binding to cyclic AMP response element-binding protein. ICG001 selectively ind
RefSeq Summary (NM_001675): This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011 ...
The cAMP-response element-binding protein (CREB) is activated by phosphorylation on serine 133 and mediates the proliferative response to a number of different signals. A mutant CREB with a serine to alanine substitution at position 133 (CREBM1) functions as a dominant-negative inhibitor. Transgenic mice that express the dominant-negative CREB protein in B lymphocytes were developed as a means to study the effects of the inhibition of CREB function on B-cell proliferation and survival. We have shown previously that CREB up-regulates Bcl-2 expression in B cells in response to activation signals. B cells from CREBM1 transgenic mice expressed lower levels of Bcl-2 with and without stimulation. Proliferation of B cells from the transgenic mice was impaired in part by lack of induction of activator protein 1 (AP1) transcription factors. B cells from the transgenic mice were more susceptible to induction of apoptosis with several different agents, consistent with the decreased expression of Bcl-2. ...
Alzheimers disease (AD) is a progressive neurodegenerative disease and the most common form of senile dementia. Recently, scientists have put significant effort into exploring the molecular mechanisms involved in the pathological processes leading to the disease. A vast number of studies have focused on understanding the nitric oxide (NO) signaling pathway, which culminates with the phosphorylation of the transcription factor cAMP-responsive element-binding protein (CREB) through the increase of the second messenger cyclic guanosine monophosphate (cGMP) and activation of cGMP-dependent protein kinase. This book chapter provides an overview of the progress being made in modulating the hippocampal synaptic transmissions, which are critical for learning and memory, by targeting the different components of the NO/cGMP/CREB phosphorylation signaling pathway. Furthermore, a description of recent research on this pathway through the use of phosphodiesterase inhibitors is emphasized.
Colorectal cancer (CRC) is the third leading cause of cancer-related death in the United States. During the tumorigenesis and metastasis of CRC, cells encounter numerous cellular and molecular events. ATF3, a member of the ATF/CREB transcription factor family, plays an important role on regulation of apoptosis and is regarded as a potential molecular target for chemoprevention and chemotherapy of colon cancer. The current study was performed to investigate cellular and molecular mechanisms by which ATF3 affects colon cancer-related phenotypes including apoptosis and metastasis. Here, we demonstrated that knockdown of ATF3 using small interfering RNA (siRNA) promotes the expression of anti-apoptotic protein, B-cell lymphoma 2 (Bcl-2), in colon cancer cells, while overexpression of ATF3 resulted in a dramatic decrease in Bcl-2 protein. Gain of function of ATF3 in colon cancer cell line HCT116 led to an increase of pro-apoptotic protein Bcl-2 homologous antagonist killer (Bak), followed by the ...
We previously demonstrated that APP epigenetically regulates Egr1 expression both in cultured neurons and in vivo. Since Egr1 is an immediate early gene involved in memory formation, we wondered whether other early genes involved in memory were regulated by APP and we studied molecular mechanisms involved. By comparing prefrontal (PF) cortex from wild type (APP+/+) and APP knockout mice (APP-/-), we observed that APP down regulates expression of four immediate early genes, Egr1, c-Fos, Bdnf and Arc. Down regulation of Egr1, c-Fos and Bdnf transcription resulted from a decreased enrichment of acetylated histone H4 on the corresponding gene promoter. Further characterization of H4 acetylation at Egr1 and c-Fos promoters revealed increased acetylation of H4K5 and H4K12 residues in APP-/- mice. Whereas APP affected Egr1 promoter activity by reducing access of the CREB transcription factor, its effect on c-Fos appeared to depend on increased recruitment of HDAC2 histone deacetylase to the gene ...
TY - JOUR. T1 - Interplay of the E box, the cyclic AMP response element, and HTF4/HEB in transcriptional regulation of the neurospecific, neurotrophin-inducible vgf gene. AU - Di Rocco, Giuliana. AU - Pennuto, Maria. AU - Illi, Barbara. AU - Canu, Nadia. AU - Filocamo, Gessica. AU - Trani, Eugenia. AU - Rinaldi, Anna Maria. AU - Possenti, Roberta. AU - Mandolesi, Georgia. AU - Sirinian, M. Isabella. AU - Jucker, Richard. AU - Levi, Andrea. AU - Nasi, Sergio. PY - 1997/3. Y1 - 1997/3. N2 - vgf is a neurotrophin response-specific, developmentally regulated gene that codes for a neurosecretory polypeptide. Its transcription in neuronal cells is selectively activated by the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor, and neurotrophin 3, which induce survival and differentiation, and not by epidermal growth factor. We studied a short region of the rat vgf promoter which is essential for its regulated expression. A cyclic AMP response element (CRE) within this region is ...
article{955774, abstract = {Among the mitogen-activated protein kinase (MAPK) targets, MSKs (mitogen- and stress-activated protein kinases) comprise a particularly interesting protein family. Because MSKs can be activated by both extracellular-signal-regulated kinase and p38 MAPKs, they are activated by many physiological and pathological stimuli. About ten years after their original discovery, they have been recognized as versatile kinases regulating gene transcription at multiple levels. MSKs directly target transcription factors, such as cAMP-response-element-binding protein and nuclear factor-kappaB, thereby enhancing their transcriptional activity. They also induce histone phosphorylation, which is accompanied by chromatin relaxation and facilitated binding of additional regulatory proteins. Here, we review the current knowledge on MSK activation and its molecular targets, focusing on recent insights into the role of MSKs at multiple levels of transcriptional regulation.}, author = ...
In addition to these posttranslational changes, an alteration in the expression of effector molecules in the DRG (66) and dorsal horn (67-69) is a prominent feature of inflammation and can be initiated in two ways. The first is as a result of an activity-dependent activation of the CREB transcription factor both in DRG and dorsal horn neurons (ref. 70; Fig. 3). As discussed above, this will result, after a delay of several hours for transcription and translation in the DRG and protein transport to central terminals, in a potentiated system in which the C fibers are primed to exert a greater effect on dorsal horn neurons as a result of an increased expression of neuromodulators like BDNF. In addition, the dorsal horn is simultaneously made hyperresponsive to such neuromodulators as a result of an activity-dependent increased expression of the TrkB receptor. The second way that transcriptional changes occur after inflammation is via the production in the inflamed tissue of specific signal ...
negative regulation of gene expression / positive regulation of CREB transcription factor activity / G-protein coupled receptor signaling pathway / regulation of sensory perception of pain / protein import into nucleus, translocation / phospholipase C-activating G-protein coupled receptor signaling pathway / cellular response to growth factor stimulus / immune response / adult locomotory behavior / G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / neuropeptide signaling pathway / adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway / synaptic transmission / regulation of calcium ion transport / cellular response to toxic substance / eating behavior / cellular response to hypoxia / negative regulation of protein oligomerization / positive regulation of peptidyl-serine phosphorylation / regulation of mitochondrial membrane potential / opioid receptor signaling pathway ...
Asthma is highly prevalent, but current therapies cannot influence the chronic course of the disease. It is thus important to understand underlying early molecular events. In this study, we aimed to use microRNAs (miRNAs) - which are critical regulators of signaling cascades - to identify so far uncharacterized asthma pathogenesis pathways. Therefore, deregulation of miRNAs was assessed in whole lungs from mice with ovalbumin (OVA)-induced allergic airway inflammation (AAI). In silico predicted target genes were confirmed in reporter assays and in house-dust-mite (HDM) induced AAI and primary human bronchial epithelial cells (NHBE) cultured at the air-liquid interface. We identified and validated the transcription factor cAMP-responsive element binding protein (Creb1) and its transcriptional co-activators (Crtc1-3) as targets of miR-17, miR-144, and miR-21. Sec14-like 3 (Sec14l3) - a putative target of Creb1 - was down-regulated in both asthma models and in NHBE cells upon IL13 treatment, while its
Extensive evidence implicates CREB-dependent gene transcription in memory (Bourtchuladze et al., 1994; Yin et al., 1994; Guzowski and McGaugh, 1997; Bartsch et al., 1998; Kida et al., 2002; Pittenger et al., 2002; Frankland et al., 2004). Multiple signaling pathways phosphorylate CREB at Ser133 (Shaywitz and Greenberg, 1999; Mayr and Montminy, 2001; Lonze and Ginty, 2002), which stimulates the recruitment of coactivators CBP/p300 (Chrivia et al., 1993; Parker et al., 1996). However, this phosphorylation event is not always sufficient to activate transcription (Impey et al., 1996; Mayr and Montminy, 2001) suggesting that CREB-mediated transcription is regulated by additional mechanisms.. In 2003, two laboratories identified a new family of CREB-specific coactivators, now referred to as CRTCs (Iourgenko et al., 2003; Conkright et al., 2003b). CRTC is thought to enhance transcription by facilitating the interaction of CREB with the RNA polymerase II pre-initiation complex (Conkright et al., 2003b; ...
TY - JOUR. T1 - Epistatic interaction of CREB1 and KCNJ6 on rumination and negative emotionality. AU - Lazary, Judit. AU - Juhasz, Gabriella. AU - Anderson, Ian M.. AU - Jacob, Christian P.. AU - Nguyen, T. Trang. AU - Lesch, Klaus Peter. AU - Reif, Andreas. AU - Deakin, J. F.William. AU - Bagdy, Gyorgy. PY - 2011/1/1. Y1 - 2011/1/1. N2 - G protein-activated K+ channel 2 (GIRK2) and cAMP-response element binding protein (CREB1) are involved in synaptic plasticity and their genes have been implicated depression and memory processing. Excessive rumination is a core cognitive feature of depression which is also present in remission. High scores on the Ruminative Response Scale (RRS) questionnaire are predictive of relapse and recurrence. Since rumination involves memory, we tested the hypothesis that variation in the genes encoding GIRK2 (KCNJ6) and CREB1 mechanisms would influence RRS scores. GIRK2 and CREB1 polymorphisms were studied in two independent samples (n = 651 and n = 1174) from the ...
The cellular transcription factor CREB (cAMP response element-binding protein) helps learning and the stabilization and retrieval of fear-based, long-term memories. This is done mainly through its expression in the hippocampus and the amygdala. Studies supporting the role of CREB in cognition include those that knock out the gene, reduce its expression, or overexpress it. Research suggests that CREB has a role in the molecular steps that stabilize memory in the brain, including that of emotional memory. Evidence of CREBs role in emotional memory falls into three experimental categories: negative manipulations (where the levels of CREB were lowered), positive manipulations (where the levels of CREB were increased), and non-interventions (where the endogenous levels of CREB were tracked before and after learning). Knockout studies in Aplysia sea slugs indicated that decreasing CREB function blocks long-term changes in synaptic function, but not short-term ones. Changes in synaptic function (i.e., ...
Flexibility in using different learning strategies was assessed in two different inbred strains of mice, the C57BL/6 and DBA/2 strains. Mice were trained sequentially in two different Morris water maze protocols that tested their ability to switch their learning strategy to complete a new task after first being trained in a different task. Training consisted either of visible platform trials (cued training) followed by subsequent hidden platform trials (place training) or the reverse sequence (place training followed by cued training). Both strains of mice showed equivalent performance in the type of training (cued or place) that they received first. However, C57BL/6 mice showed significantly better performances than DBA/2 mice following the switch in training protocols, irrespective of the order of training. After completion of the switched training session, levels of cAMP response element-binding protein (CREB) and phosphorylated CREB (pCREB) were measured in the hippocampus, striatum and prefrontal
Many human and murine tumors of distinct histology and cells of the tumor microenvironment show increased CREB expression and activity when compared with adjacent nonmalignant tissue (4), which is even more enhanced in metastasis suggesting a role for CREB in the initiation, maintenance, and progression of tumors (33, 34). This is often accompanied by poor prognosis and decreased survival rates of patients with tumor (35). Although CREB dysregulation is a critical factor in cancer development and progression, the underlying molecular mechanisms that lead to its overexpression have not yet been determined in detail. One approach to unravel this process is to determine the link(s) between oncogene expression and CREB using an in vitro model of oncogene-mediated transformation, as well as human tumor lesions with known HER-2/neu status. As shown in Fig. 7F, an important role for HER-2/neu was suggested for CREB expression and activity both in vitro and in vivo. This is based on the data ...
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ObjectiveGlucagon-like peptide-1 (GLP-1) plays a major role in pancreatic β-cell function and survival by increasing cytoplasmic cAMP levels, which are thought to affect transcription through activation of the basic leucine zipper (bZIP) transcription factor CREB. Here, we test CREB function in the adult β-cell through inducible gene deletion.MethodsWe employed cell type-specific and inducible gene ablation to determine CREB function in pancreatic β-cells in mice.ResultsBy ablating CREB acutely in mature β-cells in tamoxifen-treated CrebloxP/loxP;Pdx1-CreERT2 mice, we show that CREB has little impact on β-cell turnover, in contrast to what had been postulated previously. Rather, CREB is required for GLP-1 to elicit its full effects on stimulating glucose-induced insulin secretion and protection from cytokine-induced apoptosis. Mechanistically, we find that CREB regulates expression of the pro-apoptotic gene p21 (Cdkn1a) in β-cells, thus demonstrating that CREB is essential to mediating ...
TY - JOUR. T1 - CREB activation in the rapid, intermediate, and delayed ischemic preconditioning against hypoxic-ischemia in neonatal rat. AU - Lin, Wan Ying. AU - Chang, Ying Chao. AU - Lee, Hsueh Te. AU - Huang, Chao Ching. PY - 2009/2. Y1 - 2009/2. N2 - Ischemic preconditioning (IP) is a defense program in which exposure to sublethal ischemia followed by a period of reperfusion results in subsequent resistance to severe ischemic insults. Very few in vivo IP models have been established for neonatal brain. We examined whether rapid, intermediate, and delayed IP against hypoxic-ischemia (HI) could be induced in neonatal brain, and if so, whether the IP involved phosphorylation of cAMP response element-binding protein (pCREB) after HI. Postnatal day 7 rat pups were subjected to HI at 2 h (2-h IP), 6 h (6-h IP), or 22 h (22-h IP) after IP. We found all three IP groups had significantly reduced neuronal damage and TUNEL-(+) cells 24 h post-HI than no-IP group. Compared with control, the no-IP ...
... action potentials have been shown to induce BDNF-dependent phosphorylation of cyclic AMP response element-binding protein (CREB ... This change also affects future integration of signals, leading to at least a short-term response difference between the ... boosting synaptic responses, resetting membrane potential, retrograde actions at synapses and conditional axonal output. ...
... β-catenin and cyclic AMP (cAMP) response element binding protein (CREB), by phosphorylation. This results in a decrease in the ... Lithium was found to increase the basal levels of cyclic AMP but impair receptor coupled stimulation of cyclic AMP production.[ ... 72] It is hypothesized that the dual effects of lithium are due to the inhibition of G-proteins that mediate cyclic AMP ... Cyclic AMP secondary messengersEdit. Lithium's therapeutic effects are thought to be partially attributable to its interactions ...
... act to phosphorylate the cyclic AMP response element binding protein (CREB) transcription factor.[7] Phosphorylated CREB ... protein binding. • metalloendopeptidase inhibitor activity. • nerve growth factor receptor binding. • growth factor activity. • ... The active Ras protein phosphorylates several proteins, along with the serine/threonine kinase, Raf.[7] Raf in turn activates ... Survival and PCD mechanisms are mediated through adaptor protein binding to the death domain of the p75NTR cytoplasmic tail. ...
DNA in the cell nucleus binds to phosphorylated proteins through the cyclic AMP response element (CRE), which results in the ... Cyclic AMP-dependent protein kinases (protein kinase A) are activated by the signal chain coming from the G protein (that was ... G-protein coupled peptide receptor activity. • G-protein coupled receptor activity. • choriogonadotropin hormone binding. • ... via adenylate cyclase and cyclic AMP (cAMP). These protein kinases are present as tetramers with two regulatory units and two ...
The G-protein activates a downstream signalling cascade that causes increased level of cyclic-AMP (cAMP), which trigger ... In the olfactory system, odorant molecules in the mucus bind to G-protein receptors on olfactory cells. ... Because of the change, a change in light intensity causes the response of the rods to be much slower than expected (for a ... Koike, Takuji; Wada, Hiroshi; Kobayashi, Toshimitsu (2002). "Modeling of the human middle ear using the finite-element method ...
... inhibits G protein coupling that regulates an adenylate cyclase-mediated conversion of ATP to cyclic AMP. The end result is ... The bacterium contains a surface protein, filamentous haemagglutinin adhesin, which binds to the sulfatides found on cilia of ... This is because B. pertussis inhibits the immune response, so very little mucus is generated in the lungs. A prolonged cough ... The primers used for PCR usually target the transposable elements IS481 and IS1001. Several diagnostic tests are available, ...
... cAMP Response Element-Binding) that will bind to the CRE (cAMP Responsive Element). However, this might not occur and, instead ... binds to the upstream enhancer of the rat sodium/iodide symporter gene and participates in both thyroid-specific and cyclic-AMP ... TSH first binds to its receptors which are joined to G proteins, and then induces the activation of the enzyme adenylate ... This ingested iodine is bound to serum proteins, especially to albumins.[citation needed] The rest of the iodine which remains ...
The G-protein activates a downstream signalling cascade that causes increased level of cyclic-AMP (cAMP), which trigger ... In the olfactory system, odorant molecules in the mucus bind to G-protein receptors on olfactory cells. ... "Modeling of the human middle ear using the finite-element method". The Journal of the Acoustical Society of America. 111 (3): ... Because of the change, a change in light intensity causes the response of the rods to be much slower than expected (for a ...
... and adenyl cyclase converts AMP into cyclic AMP (cAMP) thereby down-regulating cAMP-responsive proteins involved in cell ... G protein-coupled receptors (GPCRs) such as DP2 are integral membrane proteins that, when bound by their cognate ligands (or, ... positive regulation of G-protein coupled receptor protein signaling pathway. • immune response. • neuropeptide signaling ... while arrestin-2 inhibits DP2 from further activating heterotrimeric G proteins while also linking DP2 to elements, clathrin ...
One of the nuclear targets for PKA is the transcriptional control protein CREB (cAMP response element binding protein).[5] In ... By 1972 they had evidence that the second messenger molecule cyclic AMP (cAMP) was produced in Aplysia ganglia under conditions ... By 1980, collaboration with Paul Greengard resulted in demonstration that cAMP-dependent protein kinase, also known as protein ... The Kandel lab then sought to identify proteins that had to be synthesized to convert short-term memories into long-lasting ...
... and were eventually shown to encode components of an intracellular signaling pathway involving cyclic AMP, protein kinase A, ... Calcium binds to proteins such as calmodulin (CaM) and an eye-specific protein kinase C (PKC) known as InaC. These proteins ... interact with other proteins and have been shown to be necessary for shut off of the light response. In addition, proteins ... One approach of inserting foreign genes into the Drosophila genome involves P elements. The transposable P elements, also known ...
... p38 mitogen-activated protein kinases (p38 Mpk), and cAMP response element-binding protein (CREB) which when activated ... production of cyclic AMP [cAMP]);[5] e) G proteins types to which they link and activate, i.e. those containing the Gs alpha ... G protein linkage[2]. pathways[2] Prostaglandin DP1 receptor. DP1. PGD2,,PGE2,PGF2α,PGI2=TXA2[6]. relaxant. Gs alpha subunit. ... "Prostanoid receptors - G protein-coupled receptors - IUPHAR/BPS Guide to PHARMACOLOGY". www.guidetopharmacology.org.. ...
1975). "Biologic regulation through opposing influences of cyclic GMP and cyclic AMP: the Yin Yang hypothesis". Adv Cyclic ... When cGMP binds to the allosteric GAF-B domain of the PDE, it causes conformational change in the protein structure leading to ... Furthermore, PDE2 has been indicated to play a role in inflammatory responses as it has been detected in microvessels, but not ... functional and structural elements of the active sites are very similar. The active site is formed by residues that are highly ...
... cAMP response element-binding protein) to DNA.[37] This reduction causes changes in the expression of critical immunoregulatory ... "Inhibition of the cyclic AMP signaling cascade and nuclear factor binding to CRE and kappaB elements by cannabinol, a minimally ... response to amphetamine. • G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger. • ... immune response. • nociception. • inflammatory response. • negative regulation of inflammatory response. • signal transduction ...
CREB (cAMP response element binding protein) belongs to a family of transcription factors and is positioned in the nucleus of ... Keshwani MM, Kanter JR, Ma Y, Wilderman A, Darshi M, Insel PA, Taylor SS (October 2015). "Mechanisms of cyclic AMP/protein ... The CREB protein binds to cAMP response elements CRE, and can either increase or decrease the transcription of certain genes. ... G proteins are specialised proteins whereby the nucleotides Guanosine diphosphate (GDP), and Guanosine triphosphate (GTP) bind ...
In protein synthesis, PKA first directly activates CREB, which binds the cAMP response element, altering the transcription and ... Protein kinase A, more precisely known as adenosine 3',5'-monophosphate (cyclic AMP)-dependent protein kinase was discovered by ... Cyclic+AMP-Dependent+Protein+Kinases at the US National Library of Medicine Medical Subject Headings (MeSH) ... The regulatory subunit has domains to bind to cyclic AMP, a domain that interacts with catalytic subunit and an auto inhibitory ...
... "cAMP response element binding protein" (CREB). The nucleus accumbens (NAcc) is one brain structure that has been implicated in ... Nestler EJ (August 2016). "Reflections on: "A general role for adaptations in G-Proteins and the cyclic AMP system in mediating ... In the NAcc, CREB is activated by cyclic adenosine monophosphate (cAMP) immediately after a high and triggers changes in gene ... Dependence is defined as an adaptive state that develops in response to repeated drug administration, and is unmasked during ...
Bellamacina CR «The nicotinamide dinucleotide binding motif: a comparison of nucleotide binding proteins». FASEB J., 10, 11, 01 ... Cyclic ADP-Ribose and NAADP: Structure, Metabolism and Functions. Kluwer Academic Publishers, 2002. ISBN 1-40207-281-3. ... és un element clau en el fàrmac isoniazida, que és utilitzat en el tractament de la tuberculosi, una infecció causada pel ... AMP) al fosfat 5' d'un extrem del DNA. Aquest intermediari és seguidament atacat pel grup hidroxil 3' de l'altre extrem de DNA ...
a b c d e f g h i j Shi, Q., Karuppagounder, S. S., Xu, H., Pechman, D., Chen, H., and Gibson, G. E. (2007) Responses of the ... It is an 8 monomer that can be divided into 4 domains; the FAD-binding domain (residues 1-149), the NAD+-binding domain ( ... Cofactor TPP and AMP are shown in cyan and blue, respectively. [17] (B) Diagram of the 12 subunit catalytic core of the cubic ... Declining protein levels were the most evident for the E1k and E2k subunits, when compared to the control. On contrary, as the ...
Tregs utilize beta-galactoside-binding protein to transiently inhibit PI3K/p21ras activity of human CD8+ T cells to block their ... Białko CD39 umożliwia rozkład ATP do ADP lub AMP[92]. Drugim istotnym elementem jest cząsteczka CD73, która z kolei przetwarza ... Cyclic adenosine monophosphate is a key component of regulatory T cell-mediated suppression. „J Exp Med". 204 (6), s. 1303-1310 ... Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory ...
Gαs activates the cAMP-dependent pathway by stimulating the production of cyclic AMP (cAMP) from ATP. This is accomplished by ... Metal/element. *calcium *Calcium-binding protein. *Calmodulin-binding proteins. *copper *Ceruloplasmin. *iron *Iron-binding ... G proteins, second messengers, the enzymes that trigger protein phosphorylation in response to cAMP, and consequent metabolic ... G proteins, also known as guanine nucleotide-binding proteins, are a family of proteins that act as molecular switches inside ...
As secretin binds to these receptors, it stimulates adenylate cyclase activity and converts ATP to cyclic AMP.[20] Cyclic AMP ... G-protein coupled receptor binding. • receptor binding. • protein N-terminus binding. Cellular component. • extracellular ... Secretin is released into circulation and/or intestinal lumen in response to low duodenal pH that ranges between 2 and 4.5 ... and the E-box element". Molecular Endocrinology. 18 (7): 1740-55. doi:10.1210/me.2003-0461. PMID 15118068.. ...
These proteins are called actin-binding proteins (ABP) and they are involved in actin's polymerization, depolymerization, ... Together these elements allow a spatially and temporally modulated assembly that defines a cell's response to both internal and ... "Role of actin cytoskeletal dynamics in activation of the cyclic AMP pathway and HWP1 gene expression in Candida albicans". ... The most notable of these proteins are gelsolin and cofilin. These proteins first achieve a cut by binding to an actin monomer ...
... nuclear located protein kinase C and cyclic AMP-dependent protein kinase". Frontiers in Bioscience. 13 (13): 1206-26. doi: ... In addition to serving as a primary component of cellular membranes and binding sites for intra- and intercellular proteins, ... Hunter JE (November 2006). "Dietary trans fatty acids: review of recent human studies and food industry responses". Lipids. 41 ... Protein-lipid interaction. *Phenolic lipid, a class of natural products composed of long aliphatic chains and phenolic rings ...
1975). "Biologic regulation through opposing influences of cyclic GMP and cyclic AMP: the Yin Yang hypothesis". Adv Cyclic ... When cGMP binds to the allosteric GAF-B domain of the PDE, it causes conformational change in the protein structure leading to ... Furthermore, PDE2 has been indicated to play a role in inflammatory responses as it has been detected in microvessels, but not ... functional and structural elements of the active sites are very similar. The active site is formed by residues that are highly ...
Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human ... Cyclic AMP response element-binding protein H (CREBH) was identified as a liver-specific bZIP transcription factor [10, 11]. ... The cyclic AMP response element-binding protein H (CREBH) plays important roles in hepatic lipogenesis, fatty acid oxidation, ... J. H. Lee, P. Giannikopoulos, S. A. Duncan et al., "The transcription factor cyclic AMP-responsive element-binding protein H ...
Role of cyclic AMP response element binding protein in human leukemias. Cancer 2005;104:1819-24. ... Cyclic AMP response element-binding protein overexpression: a feature associated with negative prognosis in never smokers with ... Cyclic (c)AMP responsive element binding protein (CREB) is a 43-kDa protein that belongs to the leucine-zipper class of ... miR-34b targets cyclic AMP-responsive element binding protein in acute myeloid leukemia. Cancer Res 2009;69:2471-8. ...
Cyclic AMP Response Element-Binding Protein Chemical Compounds * Transcription Chemical Compounds * Genes Chemical Compounds ... cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the pathogenesis and ... abstract = "cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the ... N2 - cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the ...
Keywords: vascular cognitive impairment, cAMP response element binding protein, cerebral ischamia, neuroprotection ... The cAMP response element binding protein (CREB) plays an important role in the mechanism of cognitive impairment and is also ... Minocycline upregulates cyclic AMP response element binding protein and brain-derived neurotrophic factor in the hippocampus of ... Minocycline upregulates cyclic AMP response element binding protein and brain-derived neurotrophic factor in the hippocampus of ...
Cyclic AMP response element-binding (CREB)-like proteins in a molluscan brain: cellular localization and learning-induced ... Cyclic AMP Response Element-Binding Protein/immunology. *Cyclic AMP Response Element-Binding Protein/metabolism* ... We demonstrate cAMP response element (CRE)-binding activity in CNS protein extracts and show that one of the CRE-binding ... cyclic adenosine 3,5-monophosphate (cAMP) response element-binding protein (CREB) are conserved key steps in the molecular ...
Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human ... J. H. Lee, P. Giannikopoulos, S. A. Duncan et al., "The transcription factor cyclic AMP-responsive element-binding protein H ... acute phase response by regulating expression of cyclic adenosine monophosphate responsive element binding protein H," ... cAMP responsive element-binding protein H)," Journal of Biological Chemistry, vol. 286, no. 49, pp. 41972-41984, 2011. View at ...
Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ... Interaction between CCAAT/Enhancer Binding Protein and Cyclic AMP Response Element Binding Protein 1 Regulates Human ...
Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ...
Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene ... Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene ... Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene ... Inhibition by antidepressant drugs of cyclic AMP response element-binding protein/cyclic AMP response element-directed gene ...
... report that CREB is present in the mitochondrial matrix of neurons and that it binds directly to cyclic AMP response elements ( ... Cyclic AMP response element-binding protein (CREB) is a widely expressed transcription factor whose role in neuronal protection ... Cyclic AMP response element-binding protein (CREB) is a widely expressed transcription factor whose role in neuronal protection ... Mitochondrial cyclic AMP response element-binding protein (CREB) mediates mitochondrial gene expression and neuronal survival. ...
Cyclic AMP Response Element-Binding Protein*Cyclic AMP Response Element-Binding Protein ... "Cyclic AMP Response Element-Binding Protein" by people in this website by year, and whether "Cyclic AMP Response Element- ... Cyclic AMP Response Element Modulator. *Cyclic AMP Response Element-Binding Protein. *Cyclic AMP Response Element-Binding ... "Cyclic AMP Response Element-Binding Protein" is a descriptor in the National Library of Medicines controlled vocabulary ...
Shift from extracellular signal-regulated kinase to AKT/cAMP response element-binding protein pathway increases survival-motor- ... the neurogenic potential of EGb 761 and its effect on cAMP response element binding protein (CREB) were examined in a double ... The transcription factor cAMP responsive element-binding protein 1 (CREB1) has a complex influence on behavioural responses to ... CREB1 and CREB-binding protein in striatal medium spiny neurons regulate behavioural responses to psychostimulants. ...
A role for cyclic AMP response element-binding protein (CREB) but not the highly similar ATF-2 protein in sterol regulation of ... A role for cyclic AMP response element-binding protein (CREB) but not the highly similar ATF-2 protein in sterol regulation of ... A role for cyclic AMP response element-binding protein (CREB) but not the highly similar ATF-2 protein in sterol regulation of ... title = "A role for cyclic AMP response element-binding protein (CREB) but not the highly similar ATF-2 protein in sterol ...
Cyclic AMP Response Element-Binding Protein. Role of CREB in Axon Growth and Regeneration. During development, several ... axon growth by regulation of gene expression via the transcription factor cyclic AMP response element-binding protein (CREB; ... cAMP response element binding protein CREB by cAMP-dependent protein kinase A and glycogen synthase kinase-3 alters DNA-binding ... and stress-activated protein kinase 1 mediates cAMP response element-binding protein phosphorylation and activation by ...
Role of cyclic AMP response element-binding protein in insulin-like growth factor-I receptor up-regulation by sex steroids in ... Role of cyclic AMP response element-binding protein in insulin-like growth factor-I receptor up-regulation by sex steroids in ... Role of cyclic AMP response element-binding protein in insulin-like growth factor-I receptor up-regulation by sex steroids in ... stim ulation with either androgens or estrogens up-regulates IGF-IR by inducing cyclic AMP response element-binding protein ( ...
Cyclic AMP Response Element Binding Protein) ELISA Kit based in Delhi, India ... Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit Rat CREB(Cyclic AMP Response Element Binding Protein) ELISA Kit ... Rat CHD3(Chromodomain Helicase DNA Binding Protein 3) ELISA Kit. *Rat CHD5(Chromodomain Helicase DNA Binding Protein 5) ELISA ... Rat MBP-C/MBL2(Mannma Binding Protein C/Mannan Binding Lectin 2) ELISA Kit ...
The second messenger cyclic AMP, for example, regulates a striking number of physiologic processes, including interme … ... Cyclic AMP / physiology* * Cyclic AMP Response Element-Binding Protein / physiology * Cyclic AMP-Dependent Protein Kinases / ... owing in large part to the characterization of cyclic AMP-responsive promoter elements, transcription factors that bind them, ... The purpose of this review is to describe the components of the cyclic AMP response unit and to analyze how these components ...
Genomic responses initiated by NMDA receptors (NMDARs) are critically dependent on whethe … ... Cyclic AMP Response Element-Binding Protein / metabolism* * Evoked Potentials * Hippocampus / growth & development* ... Genomic responses initiated by NMDA receptors (NMDARs) are critically dependent on whether synaptically or extrasynaptically ... Cyclic AMP Response Element-Binding Protein * Receptors, N-Methyl-D-Aspartate * Bicuculline ...
FIH, factor inhibiting HIF; CBP, cyclic AMP-response element binding protein; HRE, hypoxia response element ... However, in hypoxia, HIF-1α hydroxylation does not occur, stabilizing HIF-1 where it binds to a hypoxia response element ... Assuming an average cerebral tissue partial pressure of O2 (PcO2) of ~ 25 mmHg and lack of O2-binding proteins, the brains O2 ... As an element, oxygen (O) is unique; it is the third most abundant element in the universe after hydrogen and helium, the ...
Discovery of selective inhibitors for cyclic AMP response element-binding protein: a combined ligand and structure-based ... ABT-737 and erufosine combination against castration-resistant prostate cancer: a promising but cell-type specific response ...
Cyclic AMP Response Element-Binding Protein. *Culture Media, Conditioned. *Insulin-Like Growth Factor Binding Protein 1 ... G-protein coupled receptor activity - G-protein coupled receptor signaling pathway - Golgi membrane - hormone binding - ... The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5- ... Screening and Identification of a Phage Display Derived Peptide That Specifically Binds to the CD44 Protein Region Encoded by ...
cyclic AMP response element binding protein 73 ____ the co activator of CREB ... an example of a response element that binds to heat-specific transcription factors that are produced by elevated temperatures ... d. one transcription activator can bind to the same response element in multiple genes that each have unique promoters and ... a. consists of a single protein with two similar domains. b. Binding of TBP distorts DNA at TATA box. c. binding creates an 80 ...
AP1, activator protein 1; CREB, cyclic AMP response element binding protein; NFκB, nuclear factor κB; NF-Y, nuclear factor Y.. ... Bioinformatic analysis suggested that decreased activity of cyclic AMP response element binding protein and nuclear factor Y ... Cyclic AMP response element binding protein (CREB) TFBMs were significantly less prevalent in promoters of genes overexpressed ... Regulation of asthma-related T-cell cytokines by the cyclic AMP-dependent signalling pathway. Clin Exp Allergy 2000;30:920-6. ...
0/Cyclic AMP Response Element-Binding Protein; 0/Nerve Tissue Proteins; 0/RNA-Binding Proteins; 0/SMN Complex Proteins; 0/SMN1 ... Cyclic AMP Response Element-Binding Protein / genetics*. Electromyography. Female. Forearm / innervation*. Genetic ... RNA-Binding Proteins / genetics*. SMN Complex Proteins. Spain. Spinal Cord / pathology. Survival of Motor Neuron 1 Protein. ... protein, human; 0/SMN2 protein, human; 0/Survival of Motor Neuron 1 Protein; 0/Survival of Motor Neuron 2 Protein ...
Cyclic AMP response element-binding protein prevents endothelial permeability.... Source: Frontiers in Pharmacology - April 24 ... Gene Ontology Analysis for Drug Targets of the Whole Genome Transcriptome of Human Vascular Endothelial Cells in Response to ... and high ratio of hypothetical proteins with unknown functions in the class Ktedonobacteria. Furthermore, a total of 104 ...
... phosphorylated protein kinase B; CREB, cyclic AMP response element-binding protein. ... 12 The cyclic AMP response element-binding protein (CREB), which promotes continuous plastic changes and establishment of ... while the translocation of CREB binding protein (CBP) to the promoter triggered the expression of protective proteins.12,36-38 ... The protein concentration was detected with a BCA kit, and the same amount of protein was subjected to 12% sodium dodecyl ...
CREB (cyclic-AMP response element binding protein) phosphorylated and non-phosphorylated forms.. -. ... detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP). -. CREB binding protein. Acris Antibodies ... detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP). -. CREB binding protein. Acris Antibodies ... detects cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP). -. CREB binding protein. Acris Antibodies ...
2005) Serum/glucocorticoid-inducible kinase can phosphorylate the cyclic AMP response element binding protein, CREB. FEBS Lett ... 2002) A novel and conserved protein-protein interaction domain of mammalian Lin-2/CASK binds and recruits SAP97 to the lateral ... family of proteins (Sheng and Sala, 2001). It has a modular organization with several protein-protein interaction domains (i.e ... GluR1 binds to SAP97, a scaffolding protein that is a component of the postsynaptic density, via its C-terminal 7 aa. We find ...
... phosphorylated cyclic AMP response element binding protein; BDNF, brain-derived neurotrophic factor; DA, dopaminergic; MDA, ... properties and the induced upregulated expression of phosphorylated cyclic AMP response element binding protein and brain- ... sterol regulatory element-binding protein-1c, hormone-sensitive lipase and triacylglycerol hydrolase, however, tumor necrosis ... sterol regulatory element-binding protein-1c; HSL, hormone-sensitive lipase; TGH, triacylglycerol hydrolase; FAS, tumor ...
Jin, S. H., Blendy, J. A., and Thomas, S. A. (2005). Cyclic AMP response element-binding protein is required for normal ... Kreibich, A. S., and Blendy, J. A. (2004). cAMP response element-binding protein is required for stress but not cocaine-induced ... Altered sensitivity to rewarding and aversive drugs in mice with inducible disruption of cAMP response element-binding protein ... Gammie, S. C., Seasholtz, A. F., and Stevenson, S. A. (2008b). Deletion of corticotropin-releasing factor binding protein ...
One example is cyclic-AMP response element-binding protein, or CREB. In both fruit flies and sea slugs, early studies ... which help rewire the brain in response to visual input. Evolutionary biology shows that Otx2 developed from a protein in fruit ... to constantly overexpress a protein called alpha-CaM kinase II. But the proteins activity was controlled by carefully timed ... One recent example of progress in this area is Joe Tsiens work with the protein alpha-CaM kinase II in mice.5 Having shown ...
Ab161018 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA. Abcam provides free protocols, tips ... cyclic AMP response element (CRE) binding protein/activating transcription factor 1. *Cyclic AMP responsive element binding ... This protein binds to the cAMP-responsive element, an octameric palindrome. The protein interacts with host cell factor C1, ... This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this ...
  • cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the pathogenesis and maintenance of various types of human cancers. (elsevier.com)
  • In the present work, it is shown that cAMP response element binding protein (CREB) and nuclear factor-kappaB (NF-kappaB)-related antigens bind specific sequences in the Flk-1/KDR promoter. (elsevier.com)
  • Xie, F, Fan, Q, Li, BX & Xiao, X 2019, ' Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15 ', Journal of Medicinal Chemistry . (elsevier.com)
  • The activated PKA subsequently phosphorylated cAMP response element-binding protein (CREB) at Ser-133 to activate it directly, as well as indirectly through mitogen activated protein kinase p38 MAPK. (elsevier.com)
  • Relaxin increased the mRNA and protein levels of the coactivator protein PGC1α, and this effect was dependent on PKA, and was completely abrogated by a domi-nant-negative form of CREB. (elsevier.com)
  • The transcription factor cyclic AMP-responsive element binding protein (CREB) is involved in important neuronal functions, such as synaptic plasticity and neuronal survival. (biomedcentral.com)
  • Using cells stably expressing RXFP1, we found that relaxin regulation of γ activity requires accumulation of cAMP and subsequent activation of cAMP-dependent protein kinase (PKA). (elsevier.com)
  • G protein-activated K+ channel 2 (GIRK2) and cAMP-response element binding protein (CREB1) are involved in synaptic plasticity and their genes have been implicated depression and memory processing. (elsevier.com)
  • Cyclic AMP response element-binding protein H (CREBH) was identified as a liver-specific bZIP transcription factor [ 10 , 11 ]. (hindawi.com)
  • This provides a mechanism for the ligand-independent activation of γ in response to relaxin. (elsevier.com)
  • Sequence analysis suggested that putative CREBH response element (CREBHRE) is located in the human APOA5 promoter region and is highly conserved in both human and rodent. (hindawi.com)
  • Moreover, it was shown that CREBH directly regulated human APOA5 gene expression by binding to a unique CREBHRE located in the proximal human APOA5 promoter region, using 5′-deletion and mutagenesis of human APOA5 promoter analysis and chromatin immunoprecipitation assay. (hindawi.com)
  • Moreover, a recent study showed that CREBH could bind to its unique response element (CREBHRE) in the target gene promoter to modulate transcription [ 10 , 12 ]. (hindawi.com)
  • Nuclear factor-kappaB and cAMP response element binding protein mediate opposite transcriptional effects on the Flk-1/KDR gene promoter. (elsevier.com)
  • However, the regulation of FMRP by Group I mGluRs is not altered by overexpression of Ca 2+ -insensitive mutant form of downstream regulatory element antagonist modulator (DREAM), a transcriptional repressor involved in synaptic transmission and plasticity. (biomedcentral.com)
  • Consistently, inhibition by cAMP is reverted by increasing intracellular HATs and is completely abolished by site-specific mutagenesis of the cAMP response element. (elsevier.com)
  • In contrast, specific mutations in the NF-kappaB response element abolish responsiveness to p65/RelA and HATs without affecting cAMP-dependent repression. (elsevier.com)
  • A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. (childrensmercy.org)
  • Protein kinases and transcription factors transmit the signals from the external stimuli to the genome, resulting in the modification of gene expression profile of the cells to accommodate to the environmental changes. (ahajournals.org)
  • Tyrosine kinases Fyn and FAK interact with NCAM and undergo phosphorylation and this transiently activates the MAPK, ERK 1 and 2, cAMP response element binding protein (CREB) and transcription factors ELK and NFkB. (reactome.org)
  • These protein kinases are present as tetramer with two regulatory units and two catalytic units. (bionity.com)
  • These proteins are typically activated through phosphorylation by the indicated upstream kinases. (pubmedcentralcanada.ca)
  • Within this family, the salt-inducible kinases (SIKs) modulate gene expression in part via the inhibitory phosphorylation of the CRTCs, coactivators for CREB (cAMP response element-binding protein). (salk.edu)
  • The promoter elements of many monocyte-specific genes contain C/EBP binding sites, including macrophage inflammatory protein 1 alpha, tumor necrosis factor alpha ( 32 ), IL-6 ( 6 , 27 , 38 ), and IL-8 ( 27 , 36 ). (asm.org)
  • Transcription of many genes is directed by the cAMP/Ca(2+)-responsive element (CRE) and its cognate transcription factor CRE-binding protein (CREB). (aspetjournals.org)
  • Sterol regulatory element-binding proteins (SREBPs) activate promoters for key genes of metabolism to keep pace with the cellular demand for lipids. (elsevier.com)
  • RNA pol II transcribes all protein coding, snoRNA and snRNA genes d. (brainscape.com)
  • Children with asthma from a low SES background showed overexpression of genes regulating inflammatory processes, including those involved in chemokine activity, stress responses and wound responses, compared with children with asthma from a high SES background. (bmj.com)
  • 11 12 Another social factor associated with low SES-stress-has been linked to heightened inflammatory responses to allergen challenge in patients with asthma, 13 and decreased expression of genes coding hormonal receptors that regulate inflammation, including the glucocorticoid and β adrenergic receptors. (bmj.com)
  • The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. (abcam.com)
  • Processed cyclic AMP-responsive element-binding protein 3: acts as a transcription factor that activates unfolded protein response (UPR) target genes during endoplasmic reticulum (ER) stress response. (genecards.org)
  • Processed cyclic AMP-responsive element-binding protein 3: This is the transcriptionally active form that translocates to the nucleus and activates unfolded protein response (UPR) target genes during endoplasmic reticulum (ER) stress response. (genecards.org)
  • CREB (cAMP response element-binding protein) [1] is a cellular transcription factor that binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the genes. (wikiversity.org)
  • Within the cAMP-responsive element of the somatostatin gene, we observed an 8-base palindrome, 5'-TGACGTCA-3', which is highly conserved in many other genes whose expression is regulated by cAMP. (wikiversity.org)
  • Cells change the expression pattern of genes in response to extracellular signals. (ahajournals.org)
  • To obtain insights into the molecular mechanisms of this response a systematic genetic screen was performed to identify genes that when overexpressed can induce a starvation-like response in the yeast species Schizosaccharomyces pombe . (asm.org)
  • Cyclic AMP regulates the expression of a number of genes through a conserved promoter element, the CRE1. (semanticscholar.org)
  • Thus, critical insight into the role of transcription factor activation in cancer arose from studying the regulation of genes in response to cytokines under normal conditions. (aacrjournals.org)
  • Typical enhancers of viral or cellular genes are approximately 100 to 400 bp long and contain several transcription factor binding sites. (asm.org)
  • We show here that ethanol exposure causes a striking increase in CRE- and CREB-mediated gene expression in cells transfected with luciferase reporter genes and that PKA and CREB are required for this response. (aspetjournals.org)
  • CREB triggers a gene expression cascade with a group of IEGs coding for transcription factors such as zif/268, c-fos, c-jun, junB, and junD that in turn lead to transcription of late effector genes coding for proteins critical for establishing long-term memory (Abraham et al. (supermemo.com)
  • These results suggest that, in addition to its role as a transcription factor for the expression of replication genes, Mcm1 may influence the local structure of replication origins by direct binding. (labome.org)
  • Previously, we used P -element mutagenesis and quantitative trait locus (QTL) mapping in Drosophila to identify candidate genes affecting resistance to starvation stress, and variation in resistance to starvation stress between the Oregon-R (Ore) and 2b strains. (biomedcentral.com)
  • Nearly 50% of P -element insertions in 160 genes with altered transcript abundance during starvation stress had mutational effects on starvation tolerance. (biomedcentral.com)
  • MicroRNAs originate from inter-and intragenic sites including introns of protein-coding genes and their own specific gene loci. (pubmedcentralcanada.ca)
  • DNA in the cell nucleus binds to phosphorylated proteins through the cyclic AMP response element (CRE) which results in the activation of genes . (bionity.com)
  • ATF1 and CREB control transcription of many target genes through homo- or heterodimerization within the family or with other b-zip transcription factors on the sequences related to an AP1 or cAMP-response element ( Mayr and Montminy, 2001 ). (biologists.org)
  • Alberini and M.D./Ph.D. student Stephen M. Taubenfeld suspected that CREB protein depends on certain consolidation-related genes from within the region of the brain known as the hippocampus (injuries to this area often result in amnesia). (brownalumnimagazine.com)
  • Next, she says, is figuring which genes the protein switches on. (brownalumnimagazine.com)
  • This DNA contains the language (DNA or nucleotide sequence) that can be read and translated into proteins, and these areas of DNA are called genes . (bmj.com)
  • 1, 2 In higher organisms, ranging from yeast to plants and man, practically all genes are interrupted, with sequences coding for protein (coding exons ) separated by regions of non-coding DNA called introns . (bmj.com)
  • The beginning and ends of genes are usually marked by exons that do not code for parts of the protein, the so called non-coding exons. (bmj.com)
  • Ubiquitous CREB target genes are induced following recruitment of CREB and its coactivators to promoter proximal binding sites. (salk.edu)
  • Deletion of a CREB-Neurod1 bound enhancer within the super-enhancer disrupted the expression of both genes and decreased beta cell function. (salk.edu)
  • C/EBP proteins are all members of the b-ZIP family of transcription factors and share a highly homologous carboxy terminus that contains the basic and leucine zipper protein domains. (asm.org)
  • Hai T, Curran T. Cross-family dimerization of transcription factors Fos/Jun and ATF/CREB alters DNA binding specificity. (childrensmercy.org)
  • Our understanding of cyclic AMP signaling in the nucleus has expanded considerably over the past decade, owing in large part to the characterization of cyclic AMP-responsive promoter elements, transcription factors that bind them, and signal-dependent coactivators that mediate target gene induction. (nih.gov)
  • ESAT-6 decreased IFN-γ transcription and reduced expression of the transcription factors, ATF-2 and c-Jun, which normally bind to the IFN-γ proximal promoter and stimulate mRNA expression. (jimmunol.org)
  • Nuclear factor-κB and activator protein 1 are relatively well-studied redox-sensitive transcription factors involved in the various aspects of the cardiovascular disease process. (ahajournals.org)
  • Although transcription factors have not been a central focus of drug development, recent advances suggest that CREB and other such proteins may be worthwhile targets for cancer therapy. (aacrjournals.org)
  • Since normal cells are relatively insensitive to inhibition of these proteins due to redundancies in normal signaling pathways, inhibitors of these transcription factors hold the potential for having a very high therapeutic index. (aacrjournals.org)
  • Hai T. The ATF transcription factors in cellular adaptive responses. (springer.com)
  • Hai T, Hartman MG. The molecular biology and nomenclature of the ATF/CREB family of transcription factors: ATF proteins and homeostasis. (springer.com)
  • Transcription factors 1: bZIP proteins. (springer.com)
  • These fragments contained, among other sites, binding sites for AP-1 and CREB transcription factors. (asm.org)
  • CCAAT/enhancer binding proteins (C/EBPs) are six-member family (α to ζ) of transcription factors. (nature.com)
  • AP1 proteins also dimerize efficiently with other transcription factors, including some that are not members of the bZIP family 193 . (pubmedcentralcanada.ca)
  • Transcription is carried out by an enzyme called RNA polymerase II (RNA pol II) under the direction of specialised basal transcription factors that form a multi-protein complex with RNA pol II on the gene promoter . (bmj.com)
  • Binding sites for various transcription factors , which are DNA binding proteins with highly specific affinities for particular DNA sequences, sequester transcription factors to the gene where they participate in boosting (or, in some cases, repressing) the level of transcription. (bmj.com)
  • Transcription factors may bind within the promoter or lie within areas called enhancers that are located distally-usually upstream, but occasionally downstream, of the promoter. (bmj.com)
  • The transcription factor cyclic AMP-responsive element-binding protein H regulates triglyceride metabolism," Nature Medicine , vol. 17, no. 7, pp. 812-815, 2011. (hindawi.com)
  • Curcumin differentially regulates endoplasmic reticulum stress through transcriptional corepressor SMILE (small heterodimer partner-interacting leucine zipper protein)-mediated inhibition of CREBH (cAMP responsive element-binding protein H)," Journal of Biological Chemistry , vol. 286, no. 49, pp. 41972-41984, 2011. (hindawi.com)
  • RATIONALE: The transcription factor cAMP responsive element-binding protein 1 (CREB1) has a complex influence on behavioural responses to drugs of abuse which varies depending on the brain region in which it is expressed. (isharonline.org)
  • CREB3 (CAMP Responsive Element Binding Protein 3) is a Protein Coding gene. (genecards.org)
  • The cyclic (c)AMP responsive element binding protein (CREB) plays a key role in many cellular processes, including differentiation, proliferation, and signal transduction. (aacrjournals.org)
  • The phosphorylation and the binding to DNA of the nuclear transcription factor, cyclic adenosine 3',5'-monophosphate (cAMP) response element-binding protein (CREB) are conserved key steps in the molecular cascade leading to the formation of long-term memory (LTM). (nih.gov)
  • [2] CREB was first described in 1987 as a cyclic adenosine monophosphate (cAMP)-responsive transcription factor regulating the somatostatin gene. (wikiversity.org)
  • During episodes of inflammation, multiple cell types release adenine nucleotides in the form of ATP, ADP, 5′-AMP, and adenosine. (jimmunol.org)
  • Extracellular 5′-AMP is metabolized to adenosine by surface-expressed 5′-ectonucleotidase (CD73). (jimmunol.org)
  • Initial experiments revealed that adenosine and adenosine analogues induce CD73 mRNA (RT-PCR), surface expression (immunoprecipitation of surface biotinylated CD73), and function (HPLC analysis of etheno-AMP conversion to ethenoadenosine) in a time- and concentration-dependent fashion. (jimmunol.org)
  • For instance, in epithelia and endothelia, cell surface CD73 converts AMP into adenosine, which when released can activate seven-transmembrane-spanning adenosine receptors ( 3 , 4 ) or can be internalized through dipyridamole-sensitive carriers ( 5 ). (jimmunol.org)
  • Adenosine-3',5'-cyclic monophosphate (cyclic AMP) is a second messenger that plays an important role in intracellular regulation. (biovendor.com)
  • We have shown in the NG108-15 neuroblastoma × glioma hybrid cell line that ethanol increases cellular cAMP levels via activation of adenosine A 2 receptors, leading to phosphorylation of the cAMP response element-binding protein (CREB). (aspetjournals.org)
  • OBJECTIVE: The present study assessed the role of striatal CREB1 and its coactivator CREB-binding protein (CBP) in behavioural responses to psychostimulants. (isharonline.org)
  • 5 On phosphorylation at serine 133, the CREB binding protein (CBP) is recruited to CREB. (ahajournals.org)
  • Researchers led by Salvatore Oddo at the University of Texas in San Antonio found that plying Alzheimer's disease model mice with CREB binding protein (CBP) rescued learning in these mice without altering Aβ or tau levels. (alzforum.org)
  • Activator protein 1 (AP1) 1 , 2 functions in almost all areas of eukaryotic cellular behaviour, from cell cycle proliferation and development to stress response and apoptosis. (pubmedcentralcanada.ca)
  • As the induction of apoptosis by diverse exogenous signals is dependent on the elevation of intracellular Ca 2+ , the purpose of this study was to determine the role of CREB and its associated proteins in apoptosis using KCREB. (elsevier.com)
  • Most importantly, sequence variation at the ATF/CREB binding site affected basal LTR activity as well as LTR function following interleukin-6 stimulation, a treatment that leads to increases in C/EBP activation. (asm.org)
  • In particular, following activation, polymorphonuclear leukocytes release larger quantities of 5′-AMP. (jimmunol.org)
  • Analysis of DNA-binding activity by EMSA identified a functional role for CD73 cAMP response element and, moreover, indicated that multiple cAMP agonists induce transcriptional activation of functional CD73. (jimmunol.org)
  • Ebrahimian T, Dierick F, Simon D, Heidari M, Orthwein A, Mann KK, Lehoux S. FHL2 Is Essential for Spleen T Cell-Dependent B Cell Activation and Antibody Response. (harvard.edu)
  • HER-2/neu overexpression resulted in the induction and activation of CREB protein in vitro and in vivo , whereas short hairpin RNA (shRNA)-mediated inhibition of HER-2/neu correlated with downregulated CREB activity. (aacrjournals.org)
  • Increased expression of MITF and its activation by phosphorylation (P) stimulate the transcription of tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1), and dopachrome tautomerase (DCT), which produce melanin. (kegg.jp)
  • cAMP response element-binding protein (CREB) and activating transcription factor 1 (ATF-1), members of the CREB/ATF family, have been implicated in cAMP- and calcium-induced transcriptional activation. (elsevier.com)
  • When the researchers looked directly at the hippocampus, they found that normal animals averaged a 152 percent increase in CREB protein activation, but CREB levels in the forgetful animals were flat. (brownalumnimagazine.com)
  • SIRT1 activation during fasting not only relies on the increase in the NAD(+)/NADH ratio caused by energy deprivation but also involves an upregulation of SIRT1 mRNA and protein levels in various metabolic tissues. (epfl.ch)
  • We demonstrate that SIRT1 expression is controlled systemically by the activation of the cyclic AMP response-element-binding protein upon low nutrient availability. (epfl.ch)
  • In addition, CREB-1 homodimers bind to the ATF/CREB site and recruit C/EBP dimers to their cognate weak binding sites. (asm.org)
  • The cyclic AMP-regulatory dimers are degraded by phosphodiesterase and release 5'AMP. (bionity.com)
  • The different AP1 dimers bind to DNA with varying affinities and differ in their transactivation efficiencies 8 , 15 . (pubmedcentralcanada.ca)
  • Jun proteins can form stable dimers that bind to the AP1 DNA recognition element 5′-TGAC/GTCA-3′ (also known as TPA response element (TRE)) based on their ability to mediate transcriptional induction in response to the phorbol ester tumour promoter TPA 2 , 15 . (pubmedcentralcanada.ca)
  • An unusual result was that the conserved nucleotide at position 6 did not significantly affect in vitro binding of the complex. (labome.org)
  • The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. (cancerindex.org)
  • ATF1 is a transcriptional repressor of ferritin H, the major intracellular iron storage gene, through an ARE (antioxidant-responsive element). (biologists.org)
  • Ferritin is the major intracellular iron storage protein comprising 24 subunits of H and L forms ( Theil, 2003 ). (biologists.org)
  • Sequence analysis suggested that putative CREBH response element (CREBHRE) is located in the human APOA5 promoter region and is highly conserved in both human and rodent. (hindawi.com)
  • Thus, HIV-1 LTR ATF/CREB binding site sequence variation may modulate cellular signaling at the viral promoter through the C/EBP pathway. (asm.org)
  • Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and sequence-specific DNA binding . (genecards.org)
  • Each species' promoter contains a cAMP/response element (CRE) 1 consensus sequence ( [7] ) upstream of a TATA box. (wikiversity.org)
  • fnx1 + encodes a protein with sequence similarity to the proton-driven plasma membrane transporters from the multidrug resistance group of the major facilitator superfamily of proteins. (asm.org)
  • A promoter sequence which interacts with CRE binding proteins to modulate gene expression in response to the level of cAMP in the cell. (semanticscholar.org)
  • 1987 ). However, it was later found that, instead of a single polypeptide, many polypeptides can bind to the sequence (Hai et al. (springer.com)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. (uniprot.org)
  • Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus:5'- GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. (hmdb.ca)
  • It is therefore the contiguous order of the codons within a gene that delineate the linear amino acid sequence of the protein produced. (bmj.com)
  • Since CREB is a basic leucine zipper (bZip) protein, other insect members of this important family of transcriptional regulators will be briefly discussed as well. (kuleuven.be)
  • The dimeric combinations and transcriptional activity observed in vivo are largely influenced by the tissue-specific expression patterns of the individual proteins, and importantly by their specific activating mechanisms and post-translational modifications that facilitate their individual ability to dimerize with other basic leucine zipper (bZIP) domain proteins. (pubmedcentralcanada.ca)
  • AP1 constituent proteins are structurally distinguished by a basic leucine zipper (bZIP) domain that is composed of leucine zipper and basic domains. (pubmedcentralcanada.ca)
  • Cyclic AMP response element-binding (CREB)-like proteins in a molluscan brain: cellular localization and learning-induced phosphorylation. (nih.gov)
  • To induce axon extension, the cellular machinery at the nerve growth cone controls the assembly of cytoskeletal proteins and membrane components into new axons. (frontiersin.org)
  • Isoform 2 may play a role as a cellular tumor suppressor that is targeted by the hepatitis C virus (HSV) core protein. (genecards.org)
  • Binds DNA to the cAMP response element (CRE) (consensus: 5-GTGACGT[AG][AG]-3) and C/EBP sequences present in many viral and cellular promoters. (genecards.org)
  • CREB was originally isolated as a gene regulatory protein that bound to the CRE site of the somatostatin gene promoter region in response to an increase in the cellular cAMP level. (ahajournals.org)
  • ATF3, a hub of the cellular adaptive-response network, in the pathogenesis of diseases: is modulation of inflammation a unifying component? (springer.com)
  • These results indicate that SSRP1 is a novel cellular protein involved in LANA-dependent DNA replication. (labome.org)
  • Their effects are particularly important for reducing "noise" and conferring precision to cellular protein levels, particularly for low-abundance targets ( 7 , 8 ). (pubmedcentralcanada.ca)
  • This work establishes the expression of a highly conserved functional CREB1-like protein in the CNS of Lymnaea and opens the way for a detailed analysis of the role of CREB proteins in LTM formation in this model system. (nih.gov)
  • Moreover, it was shown that CREBH directly regulated human APOA5 gene expression by binding to a unique CREBHRE located in the proximal human APOA5 promoter region, using 5′-deletion and mutagenesis of human APOA5 promoter analysis and chromatin immunoprecipitation assay. (hindawi.com)
  • CREB-H: a novel mammalian transcription factor belonging to the CREB/ATF family and functioning via the box-B element with a liver-specific expression," Nucleic Acids Research , vol. 29, no. 10, pp. 2154-2162, 2001. (hindawi.com)
  • Mitochondrial cyclic AMP response element-binding protein (CREB) mediates mitochondrial gene expression and neuronal survival. (semanticscholar.org)
  • SMA is caused by a mutation of the survival-of-motor-neuron 1 (SMN1) gene, leading to a quantitative defect in the survival-motor-neuron (SMN) protein expression. (isharonline.org)
  • The purpose of this review is to describe the components of the cyclic AMP response unit and to analyze how these components cooperate to induce target gene expression in response to hormonal stimulation. (nih.gov)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • The expression of mBDNF/TrkB/PI3K pathway-related proteins was detected by Western blot and quantitative reverse transcription-polymerase chain reaction (q-RT PCR). (dovepress.com)
  • Plays also a role in the human immunodeficiency virus type 1 (HIV-1) virus protein expression and in the herpes simplex virus-1 (HSV-1) latent infection and reactivation from latency. (genecards.org)
  • Microbial infection) Plays a role in human immunodeficiency virus type 1 (HIV-1) virus protein expression. (genecards.org)
  • CREB (top) is a transcription factor capable of binding DNA (bottom) and regulating gene expression. (wikiversity.org)
  • Serial 5′ deletions and site-directed mutagenesis of IL-6 promoter along with chromatin immunoprecipitation using anti-CREB antibodies showed that cAMP response element is essential for 15(S)-HETE-induced IL-6 expression. (ahajournals.org)
  • Adenovirus-mediated transduction of 15-lipoxygenase 2 (15-LOX2) caused increased production of 15-HETE in VSMCs and enhanced IL-6 expression, SMC migration from media to intimal region, and neointima formation in response to arterial injury. (ahajournals.org)
  • Reverse transcription polymerase chain reaction, western blotting and cell transfection were applied for the measurement of mRNA, protein expression and activity. (biomedcentral.com)
  • Adaptive responses, including changes in gene expression in neurons, are thought to account for some of these complex behavioral abnormalities. (aspetjournals.org)
  • However, phosphorylation of CREB is not sufficient to activate cAMP response element (CRE)-mediated gene expression. (aspetjournals.org)
  • Coexpression of a dominant-negative CREB construct blocked ethanol-stimulated CRE-luciferase expression, further suggesting that CREB is required for this response. (aspetjournals.org)
  • Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation. (usf.edu)
  • To establish a mechanistic link between HER-2/neu-mediated transformation and CREB protein expression and function, in vitro models of HER-2/neu-overexpressing and HER-2/neu-negative/silenced counterparts as well as human mammary carcinoma lesions with defined HER-2/neu status were used. (aacrjournals.org)
  • Expression profiling in ALL: We are examining gene expression profiles in acute lymphoblastic leukemia in response to chemotherapeutic agents. (stanford.edu)
  • Guo Y, Breeden L, Zarbl H, Preston B, Eaton D. Expression of a human cytochrome p450 in yeast permits analysis of pathways for response to and repair of aflatoxin-induced DNA damage. (labome.org)
  • The CCAAT enhancer binding protein beta (C/EBP-β) increased the expression of miR-16 after I/R injury. (nature.com)
  • The ChIP and luciferase promoter assay indicated that about −1.0 kb to −0.5 kb upstream of miR-16 genome promoter region containing C/EBP-β binding motif transcriptionally regulated miR-16 expression. (nature.com)
  • No studies investigating the function of C/EBP-β in the kidney have been reported except for the ER stress induced renal C/EBP-β and the suppression of NF-κB-dependent gene expression in response to LPS in mice 17 . (nature.com)
  • T0901317, a synthetic LXR ligand, decreased AT1R mRNA and protein expression with a peak reduction at 6 hours and 12 hours of incubation, respectively. (ahajournals.org)
  • 3,4 Expression of these ABC proteins is increased by LXR agonists. (ahajournals.org)
  • MicroRNAs exert network-level effects on gene expression because each can target multiple transcripts that encode proteins in the same pathway or single targets in multiple pathways. (pubmedcentralcanada.ca)
  • 20. The expression system of claim 1, wherein the neuron-specific regulatory element is the synapsin promoter as set forth in FIG. 8 beginning at position 7 and ending at position 488. (freepatentsonline.com)
  • Radioreceptor binding assays and gene expression was done in the cerebral cortex and cerebellum of male Wistar rats using specific ligands and probes. (biomedcentral.com)
  • In general, gene expression describes the production of RNA and, subsequently, protein from a gene. (bmj.com)
  • Conversely, in the absence of energetic stress, the carbohydrate response-element-binding protein represses the expression of SIRT1. (epfl.ch)
  • Our results demonstrate how cross-talk between signal dependent and lineage determining factors promotes the expression of cell type-specific gene programs in response to extracellular cues. (salk.edu)
  • We also found that exercise significantly increased the expression of the mitochondrial uncoupling protein 2, an energy-balancing factor concerned with ATP production and free radical management. (unboundmedicine.com)
  • Kerppola TK, Curran T. Selective DNA bending by a variety of bZIP proteins. (childrensmercy.org)
  • 1989 ). The ATF2 protein consists of 505 amino acids, with phosphorylation sites near the C-terminus at serine residues 472 and 480 in the mouse protein and serine residues 490 and 498 in the human protein. (springer.com)
  • The primary function attributed to endothelial CD73 has been catabolism of extracellular nucleotides, although CD73 may also mediate lymphocyte binding under some circumstances ( 8 ). (jimmunol.org)
  • Within microtubule-associated proteins, MARKs also mediate phosphorylation of variant KXGS or ζXKXGSXXNΨ motifs, interfering with the ability of tau and MAP2/4 to bind to microtubules. (salk.edu)
  • bZIP contains the basic region and the leucine zipper important for DNA binding and dimerization, respectively. (ahajournals.org)
  • One billion years of bZIP transcription factor evolution: conservation and change in dimerization and DNA-binding site specificity. (springer.com)
  • The C/EBP family of proteins includes at least eight different proteins, many of which are important activators of transcription. (asm.org)
  • 2018 ). Thus, we can thank our (dying) lucky stars, the burning crucibles that convert hydrogen and helium into heavier elements for our O 2 , the molecule that made our world, our brains and us. (springer.com)
  • This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. (abcam.com)
  • Activating transcription factor 2 (ATF2) is a member of the leucine zipper family of DNA-binding proteins located on human chromosome 2q32 and was discovered by Maekawa et al. (springer.com)
  • Moreover, microRNA-132 (miR-132) was constitutively expressed and secreted by SVPs and remarkably upregulated, together with its transcriptional activator cyclic AMP response element-binding protein, on stimulation by H/S or vascular endothelial growth factor B. We next investigated if SVP-secreted miR-132 acts as a paracrine activator of cardiac healing. (ahajournals.org)
  • In the present study the effects of NE on the phosphorylation of the cAMP response element-binding protein (CREB) were studied to determine whether CREB phosphorylation might be involved in cAMP signal transduction in this tissue. (aspetjournals.org)
  • 1999 ). Because the consensus ATF binding site is the same as the cAMP responsive element (CRE) (Montminy and Bilezsikjian 1987 ) and because ATF1 is 75% similar. (springer.com)
  • Here we report that CREB is present in the mitochondrial matrix of neurons and that it binds directly to cyclic AMP response elements (CREs) found within the mitochondrial genome. (semanticscholar.org)
  • These findings, in conjunction with putative cAMP response elements (CREs) in the BMP2 promoter, led us to hypothesize that the PTH-CREB pathway could be a positive regulator of BMP2 transcription in osteoblasts. (nih.gov)
  • 1989 ). These proteins were collectively called the ATF family of proteins. (springer.com)
  • Single-pass type II membrane protein. (abcam.com)
  • Ecto-5′-nucleotidase (CD73) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleotides into bioactive nucleoside intermediates ( 2 ). (jimmunol.org)
  • Other research demonstrated that the protein CREB can help pinpoint the neurons linked to a particular memory. (dana.org)
  • Moreover, a recent study showed that CREBH could bind to its unique response element (CREBHRE) in the target gene promoter to modulate transcription [ 10 , 12 ]. (hindawi.com)
  • Acute and chronic ethanol exposure have been shown to modulate function of the activity-dependent gene transcription factor, cAMP-responsive element binding (CREB) protein in the brain, which may be associated with the development of alcoholism. (isharonline.org)
  • In this study, we observed the existence of a putative "megaplasmid," multiple copies of ribosomal RNA operons, and high ratio of hypothetical proteins with unknown functions in the class Ktedonobacteria. (medworm.com)
  • The term ATF was first used in 1987 to refer to a putative polypeptide with the activity to bind to the sites on the adenovirus E2, E3, and E4 promoters with sequences similar to the consensus TGACGT(C/A)(G/A) (Lee et al. (springer.com)
  • This chapter makes a journey about CMS2 tumour characterization, their conventional treatment, and how modulation of Wnt signalling or immune response may be applied to CRC therapy. (springer.com)
  • 7 Therefore, it may be possible that the LXR and Ang II/AT1R pathways functionally antagonize in terms of the inflammatory response. (ahajournals.org)
  • Previous studies reported that CCAAT/enhancer binding protein β (C/EBP β) can transactivate the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) in transient transfection analyses and that the LTR contains three binding sites for this protein ( 39 ). (asm.org)
  • Two other members of this family of proteins, chromogranin B and secretogranin II (also known as chromogranin C), contain similar CRE and TATA homologies in their proximal gene promoters (8, 9). (wikiversity.org)
  • Like D-17, the three cell lines expressed p53 proteins that were capable of activating promoters with p53 response elements on their own, and synergistically with Zhangfei. (biomedcentral.com)
  • It has been shown that M. tuberculosis inhibits IL-12 p40 gene transcription in infected macrophages ( 20 ), and this may affect the T cell responses to M. tuberculosis infection. (jimmunol.org)
  • 1,25-dihydroxyvitamin D3 suppresses renin gene transcription by blocking the activity of the cyclic AMP response element in the renin gene promoter. (semanticscholar.org)
  • Serotonin or the 5-HT 2C R preferential agonist WAY-161,503 initiated cAMP response element (CRE)-dependent gene transcription with EC 50 values of 15.5 ± 9.8 and 1.1 ± 0.9 nM, respectively. (aspetjournals.org)
  • Cyclic AMP Response Element-Binding Protein" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)
  • This interaction is reciprocal, since C/EBP dimer binding to a strong C/EBP site leads to enhanced CREB-1 recruitment to ATF/CREB sites that are weakly bound by CREB. (asm.org)
  • Vaccine development hinges on understanding the molecular mechanisms of the host-pathogen interaction, particularly those that affect T cell responses, because this is believed to be pivotal for controlling M. tuberculosis infection. (jimmunol.org)
  • It has a modular organization with several protein-protein interaction domains (i.e. (jneurosci.org)
  • It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. (abcam.com)
  • Duggan L, Hill T, Wu S, Garrison K, Zhang X, Gottlieb P. Using modified nucleotides to map the DNA determinants of the Tus-TerB complex, the protein-DNA interaction associated with termination of replication in Escherichia coli. (labome.org)
  • The mammalian AP1 proteins are homodimers and heterodimers composed of proteins from the Jun (JUN, JUNB and JUND) and Fos (FOS, FOSB, FRA1 and FRA2) families, and the closely related activating transcription factor (Atf and Creb) subfamily and the Maf subfamily 5 . (pubmedcentralcanada.ca)
  • Upon binding of cAMP to the regulatory units, the catalytic units are released and initiate the phosphorylation of proteins leading to the physiologic action. (bionity.com)
  • Here, we characterize, for the first time, a CREB1-like protein in the central nervous system (CNS) of Lymnaea, a model system used widely for the study of the fundamental mechanisms of learning and memory. (nih.gov)
  • We demonstrate cAMP response element (CRE)-binding activity in CNS protein extracts and show that one of the CRE-binding proteins is recognized by a polyclonal antibody raised to mammalian (human) CREB1. (nih.gov)
  • Moreover, phospho-CREB1-specific immunoreactivity is increased significantly in protein extracts of the CNS by forskolin, an activator of adenylate cyclase. (nih.gov)
  • Significantly, classical food-reward conditioning increases phospho-CREB1 immunoreactivity in Lymnaea CNS protein extracts. (nih.gov)
  • In response to drug exposure, CREB1 is phosphorylated in the striatum, a structure that is critically involved in reward-related learning. (isharonline.org)