AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
Cyclic AMP Response Element-Binding ProteinPhosphorylationPromoter Regions, GeneticTranscription, GeneticCyclic AMPCyclic AMP Response Element ModulatorCREB-Binding ProteinResponse ElementsSterol Regulatory Element Binding Protein 1Transcription FactorsActivating Transcription Factor 2DNA-Binding ProteinsBase SequenceActivating Transcription Factor 1Molecular Sequence DataActivating Transcription FactorsGene Expression RegulationSterol Regulatory Element Binding Protein 2Cyclic AMP-Dependent Protein KinasesNuclear ProteinsRNA, MessengerTranscriptional ActivationCell LineSignal TransductionBinding SitesLeucine ZippersRegulatory Sequences, Nucleic AcidCells, CulturedTransfectionDNAProtein BindingSterol Regulatory Element Binding ProteinsProto-Oncogene Proteins c-fosCCAAT-Enhancer-Binding ProteinsGene Products, taxColforsinGene Expression Regulation, EnzymologicGenes, ReporterG-Box Binding FactorsEnhancer Elements, GeneticAmino Acid SequenceTranscription Factor AP-1Rats, Sprague-DawleyProto-Oncogene Proteins c-junEnzyme ActivationChloramphenicol O-AcetyltransferaseMitogen-Activated Protein KinasesTumor Cells, CulturedTrans-ActivatorsDNA PrimersNF-kappa BBrain-Derived Neurotrophic Factor
Cyclic AMP Response Element-Binding Protein
A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
Phosphorylation
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Promoter Regions, Genetic
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Transcription, Genetic
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Cyclic AMP
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Cyclic AMP Response Element Modulator
Cyclic AMP response element modulator is a basic leucine zipper transcription factor that is regulated by CYCLIC AMP. It plays an important role in SPERMATID development in the mammalian TESTIS.
CREB-Binding Protein
A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
Response Elements
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.
Sterol Regulatory Element Binding Protein 1
A sterol regulatory element binding protein that regulates expression of GENES involved in FATTY ACIDS metabolism and LIPOGENESIS. Two major isoforms of the protein exist due to ALTERNATIVE SPLICING.
Transcription Factors
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Activating Transcription Factor 2
An activating transcription factor that regulates expression of a variety of GENES including C-JUN GENES; CYCLIN A; CYCLIN D1; and ACTIVATING TRANSCRIPTION FACTOR 3.
DNA-Binding Proteins
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Activating Transcription Factor 1
An activating transcription factor that regulates expression of a variety of genes including C-JUN GENES and TRANSFORMING GROWTH FACTOR BETA2.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Activating Transcription Factors
Activating transcription factors were originally identified as DNA-BINDING PROTEINS that interact with early promoters from ADENOVIRUSES. They are a family of basic leucine zipper transcription factors that bind to the consensus site TGACGTCA of the cyclic AMP response element, and are closely related to CYCLIC AMP-RESPONSIVE DNA-BINDING PROTEIN.
Gene Expression Regulation
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Sterol Regulatory Element Binding Protein 2
A sterol regulatory element binding protein that regulates GENES involved in CHOLESTEROL synthesis and uptake.
Cyclic AMP-Dependent Protein Kinases
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
Nuclear Proteins
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Transcriptional Activation
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Binding Sites
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Leucine Zippers
DNA-binding motifs formed from two alpha-helixes which intertwine for about eight turns into a coiled coil and then bifurcate to form Y shaped structures. Leucines occurring in heptad repeats end up on the same sides of the helixes and are adjacent to each other in the stem of the Y (the "zipper" region). The DNA-binding residues are located in the bifurcated region of the Y.
Regulatory Sequences, Nucleic Acid
Nucleic acid sequences involved in regulating the expression of genes.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Transfection
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Protein Binding
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Sterol Regulatory Element Binding Proteins
Sterol regulatory element binding proteins are basic helix-loop-helix leucine zipper transcription factors that bind the sterol regulatory element TCACNCCAC. They are synthesized as precursors that are threaded into the MEMBRANES of the ENDOPLASMIC RETICULUM.
Proto-Oncogene Proteins c-fos
Cellular DNA-binding proteins encoded by the c-fos genes (GENES, FOS). They are involved in growth-related transcriptional control. c-fos combines with c-jun (PROTO-ONCOGENE PROTEINS C-JUN) to form a c-fos/c-jun heterodimer (TRANSCRIPTION FACTOR AP-1) that binds to the TRE (TPA-responsive element) in promoters of certain genes.
CCAAT-Enhancer-Binding Proteins
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
Gene Products, tax
Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. The tax (trans-activator x; x is undefined) proteins act by binding to enhancer elements in the LTR.
Colforsin
Potent activator of the adenylate cyclase system and the biosynthesis of cyclic AMP. From the plant COLEUS FORSKOHLII. Has antihypertensive, positive inotropic, platelet aggregation inhibitory, and smooth muscle relaxant activities; also lowers intraocular pressure and promotes release of hormones from the pituitary gland.
Gene Expression Regulation, Enzymologic
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.
Genes, Reporter
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
G-Box Binding Factors
A family of transcription factors found primarily in PLANTS that bind to the G-box DNA sequence CACGTG or to a consensus sequence CANNTG.
Enhancer Elements, Genetic
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Transcription Factor AP-1
A multiprotein complex composed of the products of c-jun and c-fos proto-oncogenes. These proteins must dimerize in order to bind to the AP-1 recognition site, also known as the TPA-responsive element (TRE). AP-1 controls both basal and inducible transcription of several genes.
Rats, Sprague-Dawley
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Proto-Oncogene Proteins c-jun
Cellular DNA-binding proteins encoded by the c-jun genes (GENES, JUN). They are involved in growth-related transcriptional control. There appear to be three distinct functions: dimerization (with c-fos), DNA-binding, and transcriptional activation. Oncogenic transformation can take place by constitutive expression of c-jun.
Enzyme Activation
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Chloramphenicol O-Acetyltransferase
An enzyme that catalyzes the acetylation of chloramphenicol to yield chloramphenicol 3-acetate. Since chloramphenicol 3-acetate does not bind to bacterial ribosomes and is not an inhibitor of peptidyltransferase, the enzyme is responsible for the naturally occurring chloramphenicol resistance in bacteria. The enzyme, for which variants are known, is found in both gram-negative and gram-positive bacteria. EC 2.3.1.28.
Mitogen-Activated Protein Kinases
A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).
Tumor Cells, Cultured
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Trans-Activators
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
DNA Primers
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
NF-kappa B
Ubiquitous, inducible, nuclear transcriptional activator that binds to enhancer elements in many different cell types and is activated by pathogenic stimuli. The NF-kappa B complex is a heterodimer composed of two DNA-binding subunits: NF-kappa B1 and relA.
Brain-Derived Neurotrophic Factor
A member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. (From Kendrew, The Encyclopedia of Molecular Biology, 1994)

ATF-2-binding regulatory element is responsible for the Ly49A expression in murine T lymphoid line, EL-4. (1/3445)

To understand the mechanism of Ly49A-expression and its significance in T-cell differentiation, we analyzed the 5'-flanking region of the Ly49A gene in a search for the Ly49A-regulatory element. Since very few known regulatory elements have been found in this region, presumably a novel regulatory sequence(s) could exist. Accordingly, we defined the 13-bp regulatory element, 5'-ATGACGAGGAGGA-3', restricted to Ly49A-expression in EL-4 cells in comparison with two other representative cell lines tested. This element, designated as EL13, proved to be previously undiscovered by homology search and is highly homologous with several virus DNAs. Using EL13 as a probe we have cloned a cDNA encoding a binding protein to EL13. Its deduced nucleotide sequence revealed that EL13-binding protein is almost identical with rat ATF-2. Although ATF-2 is known to bind to cyclic AMP responsive element (CRE), EL13 shares five out of eight nucleotides with this consensus sequence. Our results suggested that ATF-2 may play an important role via binding to EL13 for the expression of Ly49A. These data will provide useful information for understanding T-cell and NK-cell differentiation in murine immune system.  (+info)

A critical role for cAMP response element-binding protein (CREB) as a Co-activator in sterol-regulated transcription of 3-hydroxy-3-methylglutaryl coenzyme A synthase promoter. (2/3445)

3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, a key regulatory enzyme in the pathway for endogenous cholesterol synthesis, is a target for negative feedback regulation by cholesterol. When cellular sterol levels are low, the sterol regulatory element-binding proteins (SREBPs) are released from the endoplasmic reticulum membrane, allowing them to translocate to the nucleus and activate SREBP target genes. However, in all SREBP-regulated promoters studied to date, additional co-regulatory transcription factors are required for sterol-regulated activation of transcription. We have previously shown that, in addition to SREBPs, NF-Y/CBF is required for sterol-regulated transcription of HMG-CoA synthase. This heterotrimeric transcription factor has recently been shown to function as a co-regulator in several other SREBP-regulated promoters, as well. In addition to cis-acting sites for both SREBP and NF-Y/CBF, the sterol regulatory region of the synthase promoter also contains a consensus cAMP response element (CRE), an element that binds members of the CREB/ATF family of transcription factors. Here, we show that this consensus CRE is essential for sterol-regulated transcription of the synthase promoter. Using in vitro binding assays, we also demonstrate that CREB binds to this CRE, and mutations within the CRE that result in a loss of CREB binding also result in a loss of sterol-regulated transcription. We further show that efficient activation of the synthase promoter in Drosophila SL2 cells requires the simultaneous expression of all three factors: SREBPs, NF-Y/CBF, and CREB. To date this is the first promoter shown to require CREB for efficient sterol-regulated transcription, and to require two different co-regulatory factors in addition to SREBPs for maximal activation.  (+info)

CCAAT/enhancer-binding protein beta is an accessory factor for the glucocorticoid response from the cAMP response element in the rat phosphoenolpyruvate carboxykinase gene promoter. (3/3445)

The cyclic AMP response element (CRE) of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene promoter is required for a complete glucocorticoid response. Proteins known to bind the PEPCK CRE include the CRE-binding protein (CREB) and members of the CCAAT/enhancer-binding protein (C/EBP) family. We took two different approaches to determine which of these proteins provides the accessory factor activity for the glucocorticoid response from the PEPCK CRE. The first strategy involved replacing the CRE of the PEPCK promoter/chloramphenicol acetyltransferase reporter plasmid (pPL32) with a consensus C/EBP-binding sequence. This construct, termed pDeltaCREC/EBP, binds C/EBPalpha and beta but not CREB, yet it confers a nearly complete glucocorticoid response when transiently transfected into H4IIE rat hepatoma cells. These results suggest that one of the C/EBP family members may be the accessory factor. The second strategy involved co-transfecting H4IIE cells with a pPL32 mutant, in which the CRE was replaced with a GAL4-binding sequence (pDeltaCREGAL4), and various GAL4 DNA-binding domain (DBD) fusion protein expression vectors. Although chimeric proteins consisting of the GAL4 DBD fused to either CREB or C/EBPalpha are able to confer an increase in basal transcription, they do not facilitate the glucocorticoid response. In contrast, a fusion protein consisting of the GAL4 DBD and amino acids 1-118 of C/EBPbeta provides a significant glucocorticoid response. Additional GAL4 fusion studies were done to map the minimal domain of C/EBPbeta needed for accessory factor activity to the glucocorticoid response. Chimeric proteins containing amino acid regions 1-84, 52-118, or 85-118 of C/EBPbeta fused to the GAL4 DBD do not mediate a glucocorticoid response. We conclude that the amino terminus of C/EBPbeta contains a multicomponent domain necessary to confer accessory factor activity to the glucocorticoid response from the CRE of the PEPCK gene promoter.  (+info)

CRE-mediated gene transcription in neocortical neuronal plasticity during the developmental critical period. (4/3445)

Neuronal activity-dependent processes are believed to mediate the formation of synaptic connections during neocortical development, but the underlying intracellular mechanisms are not known. In the visual system, altering the pattern of visually driven neuronal activity by monocular deprivation induces cortical synaptic rearrangement during a postnatal developmental window, the critical period. Here, using transgenic mice carrying a CRE-lacZ reporter, we demonstrate that a calcium- and cAMP-regulated signaling pathway is activated following monocular deprivation. We find that monocular deprivation leads to an induction of CRE-mediated lacZ expression in the visual cortex preceding the onset of physiologic plasticity, and this induction is dramatically downregulated following the end of the critical period. These results suggest that CRE-dependent coordinate regulation of a network of genes may control physiologic plasticity during postnatal neocortical development.  (+info)

Platelet-derived growth factor induces interleukin-6 transcription in osteoblasts through the activator protein-1 complex and activating transcription factor-2. (5/3445)

Platelet-derived growth factor (PDGF) BB, a mitogen that stimulates bone resorption, increases the expression of interleukin-6 (IL-6), a cytokine that induces osteoclast recruitment. The mechanisms involved in IL-6 induction by PDGF BB are poorly understood. We examined the effect of PDGF BB on IL-6 expression in cultures of osteoblasts from fetal rat calvariae (Ob cells). PDGF BB increased IL-6 mRNA and heterogeneous nuclear RNA levels, the rate of transcription, and the activity of base pairs (bp) -2906 to +20 IL-6 promoter fragments transiently transfected into Ob cells. Deletion analysis revealed two responsive regions, one containing an activator protein-1 (AP-1) site located between bp -276 and -257, and a second, less well defined, downstream of -257. Targeted mutations of a cyclic AMP-responsive element (CRE), and nuclear factor-IL-6 and nuclear factor-kappaB binding sites in a bp -257 to +20 IL-6 construct that was transfected into Ob cells, revealed that the CRE also contributed to IL-6 promoter induction by PDGF BB. Electrophoretic mobility shift assay revealed AP-1 and CRE nuclear protein complexes that were enhanced by PDGF BB. Supershift assays revealed binding of Jun and Fos to AP-1 and CRE sequences and binding of activating transcription factor-2 to CRE. In conclusion, PDGF BB induces IL-6 transcription in osteoblasts by regulating nuclear proteins of the AP-1 complex and activating transcription factor-2.  (+info)

pp60(v-src) induction of cyclin D1 requires collaborative interactions between the extracellular signal-regulated kinase, p38, and Jun kinase pathways. A role for cAMP response element-binding protein and activating transcription factor-2 in pp60(v-src) signaling in breast cancer cells. (6/3445)

The cyclin D1 gene is overexpressed in breast tumors and encodes a regulatory subunit of cyclin-dependent kinases that phosphorylate the retinoblastoma protein. pp60(c-src) activity is frequently increased in breast tumors; however, the mechanisms governing pp60(c-src) regulation of the cell cycle in breast epithelium are poorly understood. In these studies, pp60(v-src) induced cyclin D1 protein levels and promoter activity (48-fold) in MCF7 cells. Cyclin D1-associated kinase activity and protein levels were increased in mammary tumors from murine mammary tumor virus-pp60(c-src527F) transgenic mice. Optimal induction of cyclin D1 by pp60(v-src) involved the extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase members of the mitogen-activated protein kinase family. Cyclin D1 promoter activation by pp60(v-src) involved a cAMP response element-binding protein (CREB)/activating transcription factor 2 (ATF-2) binding site. Dominant negative mutants of CREB and ATF-2 but not c-Jun inhibited pp60(v-src) induction of cyclin D1. pp60(v-src) induction of CREB was blocked by the p38 inhibitor SB203580 or by mutation of CREB at Ser133. pp60(v-src) induction of ATF-2 was abolished by the c-Jun N-terminal kinase inhibitor JNK-interacting protein-1 or by mutation of ATF-2 at Thr69 and Thr71. CREB and ATF-2, which bind to a common pp60(v-src) response element, are transcriptionally activated by distinct mitogen-activated protein kinases. Induction of cyclin D1 activity by pp60(v-src) may contribute to breast tumorigenesis through phosphorylation and inactivation of the retinoblastoma protein.  (+info)

CREB regulates MHC class II expression in a CIITA-dependent manner. (7/3445)

The X2 box of MHC class II promoters is homologous to TRE/CRE elements and is required for expression of MHC class II genes. The X2 box-specific DNA binding activity, X2BP, was purified to homogeneity, sequenced, and identified as CREB. Transient transactivation experiments showed that CREB can cooperate with CIITA to enhance activation of transcription from MHC class II promoters in a dose-dependent manner. Binding of CREB to the class II promoter in vivo was demonstrated by a chromatin immunoprecipitation assay. Additionally, ICER, a dominant inhibitor of CREB function, was found to repress class II expression. These results demonstrate that CREB binds to the X2 box in vivo and cooperates with CIITA to direct MHC class II expression.  (+info)

Interaction of Gli2 with CREB protein on DNA elements in the long terminal repeat of human T-cell leukemia virus type 1 is responsible for transcriptional activation by tax protein. (8/3445)

The long terminal repeat (LTR) of human T-cell leukemia virus type 1 (HTLV-1) has two distinct DNA elements, one copy of TRE2S and three copies of a 21-bp sequence that respond to the viral trans-activator protein, Tax. Either multiple copies of the 21-bp sequence or a combination of one copy each of TRE2S and 21-bp sequence is required for efficient trans activation by Tax. In the trans activation of multiple copies of 21-bp sequence, CREB/ATF protein plays an essential role in forming a complex with Tax. To understand the role of TRE2S in trans activation of one copy of 21-bp sequence, we examined protein binding to the DNA elements by DNA affinity precipitation assay including Gli2 protein binding to TRE2S and CREB protein binding to 21-bp sequence. Binding of CREB to a DNA probe containing both elements, TRE2S-21bp probe, was dependent on Gli2 protein under restricted conditions and was enhanced in a dose-dependent fashion by the binding of Gli2 protein to the same probe. Mutation in either element abolished the efficient binding of CREB. A glutathione S-transferase fusion protein of a fragment of Gli2 was able to bind to CREB. Therefore, Gli2-CREB interaction on the DNA probe is proposed to stabilize CREB binding to DNA. Tax can bind to CREB protein on the DNA; therefore, stabilization of DNA binding of CREB results in more recruitment of Tax onto DNA. Conversely, Tax increased the DNA binding of CREB, although it had almost no effect on the binding of Gli2. These results suggest that Gli2 binds to the DNA element and interacts with CREB, resulting in more recruitment of Tax, which in turn stabilizes DNA binding of CREB. Similar cooperation of the protein binding to TRE2S-21bp probe was also observed in nuclear extract of an HTLV-1-infected T-cell line. Consistent with the Gli2-CREB interaction on the DNA elements, Tax-mediated trans activation was dependent on the size of the spacer between TRE2S and 21-bp sequence. The effective sizes of the spacer suggest that TRE2S in the LTR would cooperate with the second and third copies of the 21-bp sequence and contribute to trans activation of the viral gene transcription.  (+info)

The mouse CREB (cAMP responsive element binding protein) gene: structure, promoter analysis, and chromosomal localization, Co...The mouse CREB (cAMP responsive element binding protein) gene: structure, promoter analysis, and chromosomal localization, Co...
The mouse CREB (cAMP responsive element binding protein) gene: structure, promoter analysis, and chromosomal localization, Co-Author, (1992) Genomics 13 (4), 974-982 ...
https://www.taylorenglish.com/newsroom-publications-450.html
The function of cyclic-adenosine monophosphate responsive element-binding protein in hematologic malignancies - Fingerprint ...
Fingerprint Dive into the research topics of The function of cyclic-adenosine monophosphate responsive element-binding protein in hematologic malignancies. Together they form a unique fingerprint. ...
https://tmu.pure.elsevier.com/en/publications/the-function-of-cyclic-adenosine-monophosphate-responsive-element/fingerprints/
Roles of CREB in the regulation of FMRP by group I metabotropic glutamate receptors in cingulate cortex | Molecular Brain |...Roles of CREB in the regulation of FMRP by group I metabotropic glutamate receptors in cingulate cortex | Molecular Brain |...
Fragile X syndrome is caused by lack of fragile X mental retardation protein (FMRP) due to silencing of the FMR1 gene. The metabotropic glutamate receptors (mGluRs) in the central nervous system contribute to higher brain functions including learning/memory, mental disorders and persistent pain. The transcription factor cyclic AMP-responsive element binding protein (CREB) is involved in important neuronal functions, such as synaptic plasticity and neuronal survival. Our recent study has shown that stimulation of Group I mGluRs upregulated FMRP and activated CREB in anterior cingulate cortex (ACC), a key region for brain cognitive and executive functions, suggesting that activation of Group I mGluRs may upregulate FMRP through CREB signaling pathway. In this study, we demonstrate that CREB contributes to the regulation of FMRP by Group I mGluRs. In ACC neurons of adult mice overexpressing dominant active CREB mutant, the upregulation of FMRP by stimulating Group I mGluR is enhanced compared to wild-type
https://molecularbrain.biomedcentral.com/articles/10.1186/1756-6606-5-27
CAMP-responsive element-binding protein 3-like protein 4 | definition of cAMP-responsive element-binding protein 3-like protein...CAMP-responsive element-binding protein 3-like protein 4 | definition of cAMP-responsive element-binding protein 3-like protein...
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http://medical-dictionary.thefreedictionary.com/cAMP-responsive+element-binding+protein+3-like+protein+4
CAMP-responsive element-binding protein 3-like protein 1 | definition of cAMP-responsive element-binding protein 3-like protein...CAMP-responsive element-binding protein 3-like protein 1 | definition of cAMP-responsive element-binding protein 3-like protein...
Looking for online definition of cAMP-responsive element-binding protein 3-like protein 1 in the Medical Dictionary? cAMP-responsive element-binding protein 3-like protein 1 explanation free. What is cAMP-responsive element-binding protein 3-like protein 1? Meaning of cAMP-responsive element-binding protein 3-like protein 1 medical term. What does cAMP-responsive element-binding protein 3-like protein 1 mean?
http://medical-dictionary.thefreedictionary.com/cAMP-responsive+element-binding+protein+3-like+protein+1
Thiopental Inhibits the Activation of Nuclear Factor κB | Anesthesiology | American Society of AnesthesiologistsThiopental Inhibits the Activation of Nuclear Factor κB | Anesthesiology | American Society of Anesthesiologists
Total cell extracts were prepared using a high-salt detergent buffer (20 mm Hepes, pH 7.9, 350 mm NaCl, 20%[vol/vol] glycerol, 1%[wt/vol] NP-40, 1 mm MgCl2, 0.5 mm EDTA, 0.1 mm EGTA, 0.5 mm dithiothreitol, 0.1% PMSF, 1% aprotinin). Cells were harvested by centrifugation, washed once in ice-cold phosphate-buffered saline, and resuspended in four cell volumes of the detergent buffer. The cell lysate was incubated for 30 min on ice and then centrifuged for 5 min at 13,000 g at 4°C. EMSAs were performed with 32P-labeled oligonucleotides of either the inducible NF-κB or the constitutively active cyclic adenosine monophosphate response element binding protein (CREB) as previously described. 20The reaction mixture consisted of 37 μl purified water, 1 μl NF-κB or CREB oligonucleotides (25 ng/μl; Promega, Madison, WI), 5 μl kinase buffer, 5 μl γ-32P-dATP (Amersham International, Braunschweig, Germany), and 1.5 μl T4 kinase (polynucleotide kinase [PNK] buffer and PNK T4 kinase; New England ...
https://pubs.asahq.org/anesthesiology/article/96/5/1202/40067/Thiopental-Inhibits-the-Activation-of-Nuclear?searchresult=1
Coincidence signaling of dopamine D1-like and M1 muscarinic receptors in the regulation of cyclic AMP formation and CREB...Coincidence signaling of dopamine D1-like and M1 muscarinic receptors in the regulation of cyclic AMP formation and CREB...
In the prefrontal cortex, dopamine D(1)-like and M(1) muscarinic receptors are both involved in the regulation of attentional, cognitive and emotional processes but so far no information has been provided on their functional interaction. In the present study we show that in mouse medial prefrontal cortex, concomitant activation of M(1) muscarinic receptors potentiated D(1)-like receptor-induced cyclic AMP formation through a mechanism involving activation of G(q/11) and the release of G protein βγ subunits. Immunohistochemical studies indicated that the adenylyl cyclase isoforms AC2 and AC4 are expressed in mouse prefrontal cortex and that they colocalize with D(1)-like receptors with a greater association for AC4. In primary cultures of frontal cortex neurons, D(1)-like receptor-induced Ser133 phosphorylation of the transcription factor cyclic AMP-responsive element binding protein (CREB) was potentiated by concurrent stimulation of M(1) receptors. Suppression of AC4 expression with small ...
https://iris.unica.it/handle/11584/36906
Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15<...Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15<...
TY - JOUR. T1 - Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15. AU - Xie, Fuchun. AU - Fan, Qiuhua. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. PY - 2019/1/1. Y1 - 2019/1/1. N2 - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, we designed an ester prodrug of 666-15 through a long-range O,N-acyl transfer reaction for improved aqueous solubility. Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB.. AB - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, ...
https://ohsu.pure.elsevier.com/en/publications/discovery-of-a-synergistic-inhibitor-of-camp-response-element-bin
Plus itPlus it
ATM has been shown to have important roles in the transcriptional regulation of gene expression by phosphorylation of transcription factors, such as p53, nuclear factor κB, E2F, cyclic AMP-responsive element binding protein, and BRCA1, and in maintenance of the normal functions of IGF-IR and telomeres (3, 13, 14, 32). ATM-dependent gene expression has been systematically studied by comparing cell lines from normal individuals and AT patients, isogenic cell lines with restoration of ATM in AT cells or small interfering RNA knockdown of ATM in wild-type cells, and ATM+/+/ATM−/− mice (3, 13, 21-23). More than 300 genes have been reported to display ATM-dependent expression although few genes were commonly identified in two or more experiments. The microarray results presented here identified 160 genes or expressed sequence tags (∼1% of the total on the array) that were differentially expressed in normal and AT fibroblast lines under basal growth conditions, of which about half were ...
http://mcr.aacrjournals.org/content/5/8/813
Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte...Cleavage of cyclic AMP-responsive element-binding protein H aggravates myocardial hypoxia reperfusion injury in a hepatocyte...
This study aims to determine whether the proinflammatory cytokines have effects on myocardial cells (MC) and hepatocytes during myocardial ischemia to induce cyclic element-binding protein H (CREBH) cleavage AMP-responsive, enabling the acute phase response in the liver, and cause injury MCs.In superimposed on this research, transwell co-culture system hepatocyte-MC is used to investigate the relationship between the injury of hypoxia / reperfusion myocardial and CREBH cleavage. MC and neonatal rat hepatocytes derived from the ventricles and heart Sprague Dawley rats each. MC inoculated into the lower chamber of the transwell chamber for 12 hours under hypoxia. The level of endoplasmic reticulum stress protein glucose-regulated protein 78 in MC, CREBH in hepatocytes, inflammatory factors (tumor necrosis factor-α and interleukin-6) levels, and cell viability is evaluated. CREBH influence knockdown was also studied using short hairpin RNA specifically CREBH (Ad-CREBHi) .We found that ...
https://www.wetp.org/cleavage-of-cyclic-amp-responsive-element-binding-protein-h-aggravates-myocardial-hypoxia-reperfusion-injury-in-a-hepatocyte-myocardial-cell-co-culture-system/
北京大学医学部机构知识库([email protected]): cAMP Responsive Element Binding Protein-1 Is a Transcription Factor of Lysosomal-Associated Protein...
Lysosomal-associated protein transmembrane-4 beta (LAPTM4B) is a potential proto-oncogene, whose overexpression is involved in cancer occurrence and progression. Its transcript is up-regulated in various types of solid tumors including breast cancer. However, its transcriptional regulation mechanism is still unclear. To investigate the mechanism of transcriptional regulation of LAPTM4B in human breast cancer cells, a series of luciferase reporter constructs and construct with mutated binding site for cAMP responsive element binding protein-1 (CREB1) were generated by PCR amplification and transiently transfected into breast cancer cells to determine the transcriptional activities of different promoter regions. The + 10 similar to+ 292 promoter region was possessed the highest transcriptional activity. The ability of CREB1 to bind the LAPMT4B promoter was confirmed by electrophoretic mobility shift assay, super-shift and RNA interference experiments. Our study identified the core promoter region ...
http://ir.bjmu.edu.cn/handle/400002259/55826
Creb1 - Cyclic AMP-responsive element-binding protein 1 - Rattus norvegicus (Rat) - Creb1 gene & proteinCreb1 - Cyclic AMP-responsive element-binding protein 1 - Rattus norvegicus (Rat) - Creb1 gene & protein
Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity).
http://www.uniprot.org/uniprot/P15337
Design, synthesis and biological evaluation of regioisomers of 666-15 as inhibitors of CREB-mediated gene transcription<...Design, synthesis and biological evaluation of regioisomers of 666-15 as inhibitors of CREB-mediated gene transcription<...
TY - JOUR. T1 - Design, synthesis and biological evaluation of regioisomers of 666-15 as inhibitors of CREB-mediated gene transcription. AU - Xie, Fuchun. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. N1 - Funding Information: This work was made possible through financial supports provided by the United States National Institutes of Health ( R01GM087305 ) and OHSU Office of Technology Transfer and Business Development . PY - 2017/2/1. Y1 - 2017/2/1. N2 - cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the pathogenesis and maintenance of various types of human cancers. Identification of small molecule inhibitors of CREB-mediated gene transcription has been pursued as a novel strategy for developing cancer therapeutics. We recently discovered a potent and cell-permeable CREB inhibitor called 666-15. 666-15 is a bisnaphthamide and has been shown to possess efficacious anti-breast cancer activity without toxicity in vivo. In this study, we ...
https://ohsu.pure.elsevier.com/en/publications/design-synthesis-and-biological-evaluation-of-regioisomers-of-666
Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human...Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human...
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
https://www.hindawi.com/journals/bmri/2013/892491/
Nuclear factor-kappaB and cAMP response element binding protein mediate opposite transcriptional effects on the Flk-1/KDR gene...
TY - JOUR. T1 - Nuclear factor-kappaB and cAMP response element binding protein mediate opposite transcriptional effects on the Flk-1/KDR gene promoter.. AU - Illi, B.. AU - Puri, P.. AU - Morgante, L.. AU - Capogrossi, M. C.. AU - Gaetano, C.. PY - 2000/6/23. Y1 - 2000/6/23. N2 - -The vascular endothelial growth factor receptor Flk-1/KDR is highly expressed during development and almost disappears in adult tissues. Despite its biological relevance, little is known about the molecular mechanisms controlling its expression. In the present work, it is shown that cAMP response element binding protein (CREB) and nuclear factor-kappaB (NF-kappaB)-related antigens bind specific sequences in the Flk-1/KDR promoter. Functional studies demonstrate that cAMP represses whereas tumor necrosis factor-alpha, an activator of NF-kappaB, stimulates promoter activity. Histone acetyltransferases (HATs) P/CAF and CBP/p300 together with p65/RelA, the catalytic subunit of NF-kappaB, increase Flk-1/KDR promoter ...
https://moh-it.pure.elsevier.com/en/publications/nuclear-factor-kappab-and-camp-response-element-binding-protein-m
Volume 11.20 | May 31 - Cell Therapy News
The results reveal an essential survival pathway in malignant glioma, whereby activation of a RAS-mitogen-activated protein kinase or phosphoinositide-3-kinase signaling cascade leads to induction of the transcription factor cAMP response element-binding protein-3-like-2 (CREB3L2), which directly activates ATF5 expression. ATF5, in turn, promotes survival by stimulating transcription of myeloid cell leukemia sequence-1 (MCL1), an antiapoptotic B cell leukemia-2 family member. [Nat Med ...
https://www.celltherapynews.com/issue/volume-11-20-may-31/
A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor...A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor...
Read A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor and associates with ABA signaling, Plant Molecular Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
https://www.deepdyve.com/lp/springer_journal/a-rice-dehydration-inducible-snf1-related-protein-kinase-2-JNQszofmZS
Relaxin activates peroxisome proliferator-activated receptor γ(PPAR γ)through a pathway involving PPAR γ coactivator 1α (PGC1α)...
TY - JOUR. T1 - Relaxin activates peroxisome proliferator-activated receptor γ(PPAR γ)through a pathway involving PPAR γ coactivator 1α (PGC1α). AU - Singh, Sudhir. AU - Simpson, Ronda L.. AU - Bennett, Robert G.. PY - 2015/1/9. Y1 - 2015/1/9. N2 - Relaxin activation of its receptor RXFP1 triggers multiple signaling pathways. Previously, we have shown that relaxin activates &gamma transcriptional activity in a ligand-independent manner, but the mechanism for this effect was unknown. In this study, we examined the signaling pathways of downstream of RXFP1 leading to γ activation. Using cells stably expressing RXFP1, we found that relaxin regulation of &gamma activity requires accumulation of cAMP and subsequent activation of cAMP-dependent protein kinase (PKA). The activated PKA subsequently phosphorylated cAMP response element-binding protein (CREB) at Ser-133 to activate it directly, as well as indirectly through mitogen activated protein kinase p38 MAPK. Activated CREB was required for ...
https://nebraska.pure.elsevier.com/en/publications/relaxin-activates-peroxisome-proliferator-activated-receptor-%CE%B3ppa
Active Motif » TransAM CREB (cyclic AMP response element binding, CREB and Phosphorylated CREB (Ser133) DNA binding ELISAs)Active Motif » TransAM CREB (cyclic AMP response element binding, CREB and Phosphorylated CREB (Ser133) DNA binding ELISAs)
TransAM CREB and TransAM pCREB Kits are DNA-binding ELISAs that quanify the activated transcription factors using a method that is faster and more sensitive than gelshift, without radioactivity and gels.
http://www.activemotif.jp/catalog/192/transam-creb-pcreb
Ethanol sensitivity: a central role for CREB transcription regulation in the cerebellum
Background: Lowered sensitivity to the effects of ethanol increases the risk of developing alcoholism. Inbred mouse strains have been useful for the study of the genetic basis of various drug addiction-related phenotypes. Inbred Long-Sleep (ILS) and Inbred Short-Sleep (ISS) mice differentially express a number of genes thought to be implicated in sensitivity to the effects of ethanol. Concomitantly, there is evidence for a mediating role of cAMP/PKA/CREB signalling in aspects of alcoholism modelled in animals. In this report, the extent to which CREB signalling impacts the differential expression of genes in ILS and ISS mouse cerebella is examined. Results: A training dataset for Machine Learning (ML) and Exploratory Data Analyses (EDA) was generated from promoter region sequences of a set of genes known to be targets of CREB transcription regulation and a set of genes whose transcription regulations are potentially CREB-independent. For each promoter sequence, a vector of size 132, with ...
http://connection.ebscohost.com/c/articles/28858743/ethanol-sensitivity-central-role-creb-transcription-regulation-cerebellum
crebl2 Protein, cAMP responsive element binding protein-like 2 - Creative BioMart
cAMP response element (CRE)-binding protein-like-2 (CREBL2) was identified in a search to find genes in a commonly deleted region on chromosome 12p13 flanked by ETV6 and CDKN1B genes, frequently associated with hematopoietic malignancies, as well as breast, non-small-cell lung and ovarian cancers. CREBL2 shares a 41% identity with CRE-binding protein (CREB) over a 48-base long region which encodes the bZip domain of CREB. The bZip domain consists of about 30 amino acids rich in basic residues involved in DNA binding, followed by a leucine zipper motif involved in protein dimerization. This suggests that CREBL2 encodes a protein with DNA binding capabilities. The occurance of CREBL2 deletion in malignancy suggests that CREBL2 may act as a tumor suppressor gene.
https://www.creativebiomart.net/symbolsearch_CREBL2.htm
Epigenetic upregulation of PSD-95 contributes to the rewarding behavior by morphine conditioning. - Semantic ScholarEpigenetic upregulation of PSD-95 contributes to the rewarding behavior by morphine conditioning. - Semantic Scholar
Abuse of opiates, including morphine, induced remarkable synaptic adaptation in several brain regions including ventral tegmental area (VTA), which underlay the induction and maintenance of opioid dependence and addiction. Scaffolding protein postsynaptic density protein 95 (PSD-95) is critically involved in the glutamatergic synaptic maturation and plasticity in the central neurons. The present study revealed a significantly increased mRNA and protein expression of PSD-95 in the VTA of the rats conditioned with morphine. The further chromatin immunoprecipitation study found an increased histone H3 acetylation in the promoter region of Dlg4. An upregulation of expression of phosphorylated cAMP response element-binding protein (pCREB) and the occupancy of pCREB in the Dlg4 promoter region were shown in the VTA of the morphine-conditioned rats. Inhibition of pCREB activity significantly decreased the histone H3 acetylation in Dlg4 promoter region, PSD-95 upregulation, enhancement of glutamatergic strength
https://www.semanticscholar.org/paper/Epigenetic-upregulation-of-PSD-95-contributes-to-t-Wang-Yan/0efc2f1d59fa22abdf3ca276a35717f5b06797b0
Transcription Factor Assisted Loading and Enhancer Dynamics Dictate the Hepatic Fasting Response - Philippine Genome CenterTranscription Factor Assisted Loading and Enhancer Dynamics Dictate the Hepatic Fasting Response - Philippine Genome Center
Fasting elicits transcriptional programs in hepatocytes leading to glucose and ketone production. This transcriptional program is regulated by many transcription factors (TFs). To understands how this complex network regulates the metabolic response to fasting we aimed at isolating the enhancers and TFs dictating it. Measuring chromatin accessibility revealed that fasting massively reorganizes liver chromatin, exposing numerous fasting-induced enhancers. By utilizing computational methods in combination with dissecting enhancers features and TF cistromes, we implicated four key TFs regulating the fasting response: glucocorticoid receptor (GR), cAMP responsive element binding protein 1 (CREB1), peroxisome proliferator activated receptor alpha (PPARA) and CCAAT/enhancer binding protein beta (CEBPB). These TFs regulate fuel production by two distinctly-operating modules, each controlling a separate metabolic pathway. The gluconeogenic module operates through assisted loading whereby GR doubles the ...
https://pgc.up.edu.ph/transcription-factor-assisted-loading-and-enhancer-dynamics-dictate-the-hepatic-fasting-response/
anti-CREB3L1 antibody [C3], C-term | GeneTexanti-CREB3L1 antibody [C3], C-term | GeneTex
CREB3L1 antibody [C3], C-term (cAMP responsive element binding protein 3-like 1) for WB. Anti-CREB3L1 pAb (GTX104818) is tested in Human samples. 100% Ab-Assurance.
http://www.genetex.com/CREB3L1-antibody-C3-C-term-GTX104818.html
Early Odor Preference Learning in the Rat: Bidirectional Effects of cAMP Response Element-Binding Protein (CREB) and Mutant...Early Odor Preference Learning in the Rat: Bidirectional Effects of cAMP Response Element-Binding Protein (CREB) and Mutant...
Early odor preference learning in rats is associated with increases of phosphorylated CREB (pCREB) in mitral cells of the olfactory bulb. In the present study, herpes simplex virus expressing CREB (HSV-CREB) and dominant-negative mutant CREB (HSV-mCREB) have been injected into the bulb to assess a causal role for CREB and pCREB in this model. Odor paired with stroking or with the β-adrenoceptor agonist isoproterenol produces odor approach 24 hr later. Isoproterenol-induced learning exhibits an inverted U curve dose-dependent learning relationship with both low and high doses failing to produce learning. pCREB increases have only been seen at the learning effective dose. In the present study, injection of an HSV vector expressing mutant CREB into the olfactory bulb prevented learning induced by stroking. Control HSV expressing LacZ was without effect. Expression of mutant CREB shifted the dose-learning curve for isoproterenol to the right such that a higher dose was required to induce learning. ...
http://research.library.mun.ca/469/
Importance of cAMP-response element-binding protein in regulation of expression of the murine cyclic nucleotide...Importance of cAMP-response element-binding protein in regulation of expression of the murine cyclic nucleotide...
Incubation of 3T3-L1 preadipocytes with isobutylmethylxanthine (IBMX), dexamethasone, and insulin, alone or in combination, demonstrated that IBMX, which increased cAMP-response element-binding protein (CREB) phosphorylation, was the predominant regulator of Pde3b expression. Real time PCR and immunoblotting indicated that in 3T3-L1 preadipocytes, IBMX-stimulated induction of Pde3b mRNA and protein was markedly inhibited by dominant-negative CREB proteins. By transfecting preadipocytes, differentiating preadipocytes, and HEK293A cells with luciferase reporter vectors containing different fragments of the 5- flanking region of the Pde3b gene, we identified a distal promoter that contained canonical cis-acting cAMP-response elements (CRE) and a proximal, GC-rich promoter region, which contained atypical CRE. Mutation of the CRE sequences dramatically reduced distal promoter activity; H89 inhibited IBMX-stimulated CREB phosphorylation and proximal and distal promoter activities. Distal promoter ...
https://www.ludc.lu.se/eva-degerman-pi/publication/22b14732-23a7-49da-acf7-b7dd55095f70
Directly Modulating Glucose Homeostasis | Science SignalingDirectly Modulating Glucose Homeostasis | Science Signaling
The concentration of glucose in the bloodstream is regulated by glucose itself, along with the hormones insulin and glucagon. Glucagon stimulates gluconeogenesis in part by regulating phosphorylation of a transcriptional coactivator known as cyclic adenosine monophosphate response element-binding protein 2 (CRTC2). Dentin et al. (see the Perspective by Birnbaum) found that high concentrations of circulating glucose also regulate CRTC2, but do so through stimulation of the hexosamine biosynthetic pathway and consequent O-linked glycosylation of the same serine residue in CRTC2 that is modified by phosphorylation. Thus, CRTC2 integrates signals from hormones and nutrients and might be a target for efforts to treat abnormalities of glucose homeostasis that are associated with diabetes.. R. Dentin, S. Hedrick, J. Xie, J. Yates, III, M. Montminy, Hepatic glucose sensing via the CREB coactivator CRTC2. Science 319, 1402-1405 (2008). [Abstract] [Full Text]. M. J. Birnbaum, Sweet conundrum. Science 319, ...
http://stke.sciencemag.org/content/1/10/ec93
4-PPBP - Wikipedia4-PPBP - Wikipedia
4-PPBP is a molecule which binds to sigma receptors.[1] 4-PPBP decreases neuronal nitric oxide synthase (nNOS) activity and ischemia-evoked nitric oxide (NO) production. 4-PPBP provides neuroprotection; this involves the prevention of ischemia-induced intracellular Ca2+dysregulation.[2]4-PPBP protects neurons using a mechanism that activates the transcription factor cyclic adenosine monophosphate response element-binding protein (CREB). Neuroprotection that is associated with 4-PPBP increases Bcl-2 expression; Bcl-2 expression is regulated by CREB. [3] ...
https://en.wikipedia.org/wiki/4-PPBP
JCI - Cancer-associated fibroblasts regulate endothelial adhesion protein LPP to promote ovarian cancer chemoresistanceJCI - Cancer-associated fibroblasts regulate endothelial adhesion protein LPP to promote ovarian cancer chemoresistance
Next, we focused on intermediate signaling molecules that are implicated in the mediation of cell motility and calcium signaling. Compared with control cells, MECs treated with recMFAP5 had higher expression of phosphorylated FAK (p-FAK) (Y861), p-PLC-γ1 (Y783), p-PKCθ (T538), p-ERK1/2 (T202/Y204), phosphorylated myosin regulatory light chain 2 (p-MLC2) (T18/S19), phosphorylated cyclic AMP-responsive element-binding protein (p-CREB) (S133), c-Jun, and p-c-Jun (S73), which may have led to the upregulation of LPP expression and thus increased cell motility and permeability (Figure 7G).. Because our data demonstrated that MFAP5-induced microvascular endothelial cell motility was suppressed in cells that had been pretreated with an anti-αVβ3 integrin antibody (Figure 7H) and that MFAP5-upregulated p-FAK (Y861) expression was suppressed in cells that had been pretreated with BAPTA-AM (1,2-bis-[2-aminophenoxy]-ethane-N,N,N′,N′-tetraacetic acid, tetraacetoxymethyl ester) (Supplemental Figure 9A ...
https://www.jci.org/articles/view/95200
cAMP Response Element-Binding Protein Is Required for Stress But Not Cocaine-Induced Reinstatement at The University of...
cAMP Response Element-Binding Protein Is Required for Stress But Not Cocaine-Induced Reinstatement is an eagle-i resource of type Journal article at The University of Pennsylvania.
http://eagle-i.itmat.upenn.edu/i/00000141-3cc3-e792-91c7-0c6080000000
Anti CREB1 Antibody | Bio-Rad Antibodies (formerly AbD Serotec)Anti CREB1 Antibody | Bio-Rad Antibodies (formerly AbD Serotec)
Rabbit anti CREB1 antibody recognizes cyclic AMP-responsive element-binding protein 1 (CREB1). CREB1 is a transcription factor that has be
https://www.bio-rad-antibodies.com/rat-creb1-antibody-ahp2300.html
Heme Deficiency Interferes with the Ras-Mitogen-activated Protein Kinase Signaling Pathway and Expression of a Subset of...
Defective heme synthesis in mammals has been suspected of causing neuropathy associated with porphyrias and lead poisoning. To determine the molecular action of heme in neuronal cells, we examined the effect of the inhibition of heme synthesis on nerve growth factor (NGF) signaling in PC12 cells. We found that the inhibition of heme synthesis by succinyl acetone interferes with NGF-induced neurite outgrowth in PC12 cells. Furthermore, we show that heme deficiency obliterates the activation of the signaling intermediates of the Ras-mitogen-activated protein kinase signaling pathway and its downstream target, the transcription activator cyclic AMP response element-binding protein. Strikingly, microarray expression analysis shows that the inhibition of heme synthesis selectively diminishes the induction of expression of a subset of neuron-specific genes by NGF, such as Ras and neurofilament proteins, whereas NGF induces the expression of several major classes of neuronal genes that encode ...
http://cgd.aacrjournals.org/cgi/content/abstract/13/9/431
Autocrine Activation of Neuronal NMDA Receptors by Aspartate Mediates Dopamine- and cAMP-Induced CREB-Dependent Gene...Autocrine Activation of Neuronal NMDA Receptors by Aspartate Mediates Dopamine- and cAMP-Induced CREB-Dependent Gene...
Our hypothesis is based on several lines of evidence. First, the effects of cAMP and DA on both 4xCRE (Figs. 3a,b, 5) and c-fos and BDNF mRNA expression (Fig. 6) are blocked by NMDAR antagonists. Second, two structurally distinct inhibitors of neuronal EAA uptake, TBOA (Fig. 7) and trans-PDC (supplemental Fig. S3, available at www.jneurosci.org as supplemental material), potentiated the stimulation of gene transcription by cAMP. Third, the aspartate+glutamate-, but not the glutamate-only-, scavenging system abolished stimulation of CREB-dependent gene transcription by forskolin (Fig. 8); the aspartate-scavenging enzyme, GOT, degrades l- but not d-aspartate demonstrating that l-aspartate is the active extracellular EAA in this signaling pathway. Finally, forskolin was found to induce release of aspartate but not glutamate (Fig. 9). Together, these results lead to the conclusion that cAMP-induced release of aspartate and the resulting activation of NR2B-containing NMDARs mediate the effects of ...
http://www.jneurosci.org/content/29/40/12702.long
Degradation of cAMP-responsive element-binding protein by the ubiquitin-proteasome pathway contributes to glucotoxicity in beta...
In type 2 diabetes, chronic hyperglycemia is detrimental to beta-cells, causing apoptosis and impaired insulin secretion. The transcription factor cAMP-responsive element-binding protein (CREB) is crucial for beta-cell survival and function. We inves
http://www.biomedsearch.com/nih/Degradation-cAMP-responsive-element-binding/19223597.html
ICG001/ICG-001/ICG 001/847591-62-2/acesobio supply
CG-001 is a selective Wnt/β-catenin signalling inhibitor with an IC50 of 3μM. ICG 001, a small molecule that down-regulates beta-catenin/T cell factor signaling by specifically binding to cyclic AMP response element-binding protein. ICG001 selectively ind
http://acesobio.com/e_productshow/?303-ICG-001-303.html
Human Gene ATF4 (ENST00000337304.2) Description and Page IndexHuman Gene ATF4 (ENST00000337304.2) Description and Page Index
RefSeq Summary (NM_001675): This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011 ...
http://genome.ucsc.edu/cgi-bin/hgGene?hgg_gene=ENST00000337304.2&hgg_prot=uc003aya.4&hgg_chrom=chr22&hgg_start=39520563&hgg_end=39522683&hgg_type=knownGene&db=hg38&hgsid=792032863_QAgkGU0ca3pjHwdSeLxJbUe0TjIP
Impaired proliferation and survival of activated B cells in transgenic mice that express a dominant-negative cAMP-response...Impaired proliferation and survival of activated B cells in transgenic mice that express a dominant-negative cAMP-response...
The cAMP-response element-binding protein (CREB) is activated by phosphorylation on serine 133 and mediates the proliferative response to a number of different signals. A mutant CREB with a serine to alanine substitution at position 133 (CREBM1) functions as a dominant-negative inhibitor. Transgenic mice that express the dominant-negative CREB protein in B lymphocytes were developed as a means to study the effects of the inhibition of CREB function on B-cell proliferation and survival. We have shown previously that CREB up-regulates Bcl-2 expression in B cells in response to activation signals. B cells from CREBM1 transgenic mice expressed lower levels of Bcl-2 with and without stimulation. Proliferation of B cells from the transgenic mice was impaired in part by lack of induction of activator protein 1 (AP1) transcription factors. B cells from the transgenic mice were more susceptible to induction of apoptosis with several different agents, consistent with the decreased expression of Bcl-2. ...
https://www.pubfacts.com/detail/12374787/Impaired-proliferation-and-survival-of-activated-B-cells-in-transgenic-mice-that-express-a-dominant-
Browsing Chester Medical School by Authors
Bone marrow-derived mesenchymal stem cells (BMSC) modulate inflammatory/immune responses and promote motor functional recovery after spinal cord injury (SCI). However, the effects of BMSC transplantation on central neuropathic pain and neuronal hyperexcitability after SCI remain elusive. This is of importance because BMSC-based therapies have been proposed for clinical treatment. We investigated the effects of BMSC transplantation on pain hypersensitivity in green fluorescent protein (GFP)-positive bone marrow-chimeric mice subjected to a contusion SCI, and the mechanisms of such effects. BMSC transplantation at day 3 post-SCI improved motor function and relieved SCI-induced hypersensitivities to mechanical and thermal stimulation. The pain improvements were mediated by suppression of protein kinase C-γ and phosphocyclic AMP response element binding protein expression in dorsal horn neurons. BMSC transplants significantly reduced levels of p-p38 mitogen-activated protein kinase and ...
https://chesterrep.openrepository.com/handle/10034/337531/browse?type=author&value=Sugita%2C+Daisuke
Targeting the NO/cGMP/CREB Phosphorylation Signaling Pathway in Alzheimers Disease | IntechOpenTargeting the NO/cGMP/CREB Phosphorylation Signaling Pathway in Alzheimers Disease | IntechOpen
Alzheimers disease (AD) is a progressive neurodegenerative disease and the most common form of senile dementia. Recently, scientists have put significant effort into exploring the molecular mechanisms involved in the pathological processes leading to the disease. A vast number of studies have focused on understanding the nitric oxide (NO) signaling pathway, which culminates with the phosphorylation of the transcription factor cAMP-responsive element-binding protein (CREB) through the increase of the second messenger cyclic guanosine monophosphate (cGMP) and activation of cGMP-dependent protein kinase. This book chapter provides an overview of the progress being made in modulating the hippocampal synaptic transmissions, which are critical for learning and memory, by targeting the different components of the NO/cGMP/CREB phosphorylation signaling pathway. Furthermore, a description of recent research on this pathway through the use of phosphodiesterase inhibitors is emphasized.
https://www.intechopen.com/books/neurochemical-basis-of-brain-function-and-dysfunction/targeting-the-no-cgmp-creb-phosphorylation-signaling-pathway-in-alzheimer-s-disease
THE POTENTIAL DICHOTOMOUS ROLE OF ACTIVATING TRANSCRIPTION FACTOR 3 (ATF3) IN COLON CANCERTHE POTENTIAL DICHOTOMOUS ROLE OF ACTIVATING TRANSCRIPTION FACTOR 3 (ATF3) IN COLON CANCER
Colorectal cancer (CRC) is the third leading cause of cancer-related death in the United States. During the tumorigenesis and metastasis of CRC, cells encounter numerous cellular and molecular events. ATF3, a member of the ATF/CREB transcription factor family, plays an important role on regulation of apoptosis and is regarded as a potential molecular target for chemoprevention and chemotherapy of colon cancer. The current study was performed to investigate cellular and molecular mechanisms by which ATF3 affects colon cancer-related phenotypes including apoptosis and metastasis. Here, we demonstrated that knockdown of ATF3 using small interfering RNA (siRNA) promotes the expression of anti-apoptotic protein, B-cell lymphoma 2 (Bcl-2), in colon cancer cells, while overexpression of ATF3 resulted in a dramatic decrease in Bcl-2 protein. Gain of function of ATF3 in colon cancer cell line HCT116 led to an increase of pro-apoptotic protein Bcl-2 homologous antagonist killer (Bak), followed by the ...
https://drum.lib.umd.edu/handle/1903/17382
Epigenetic regulations of immediate early genes expression involved in memory formation by the amyloid precursor protein of...Epigenetic regulations of immediate early genes expression involved in memory formation by the amyloid precursor protein of...
We previously demonstrated that APP epigenetically regulates Egr1 expression both in cultured neurons and in vivo. Since Egr1 is an immediate early gene involved in memory formation, we wondered whether other early genes involved in memory were regulated by APP and we studied molecular mechanisms involved. By comparing prefrontal (PF) cortex from wild type (APP+/+) and APP knockout mice (APP-/-), we observed that APP down regulates expression of four immediate early genes, Egr1, c-Fos, Bdnf and Arc. Down regulation of Egr1, c-Fos and Bdnf transcription resulted from a decreased enrichment of acetylated histone H4 on the corresponding gene promoter. Further characterization of H4 acetylation at Egr1 and c-Fos promoters revealed increased acetylation of H4K5 and H4K12 residues in APP-/- mice. Whereas APP affected Egr1 promoter activity by reducing access of the CREB transcription factor, its effect on c-Fos appeared to depend on increased recruitment of HDAC2 histone deacetylase to the gene ...
https://dial.uclouvain.be/pr/boreal/object/boreal:152151
Interplay of the E box, the cyclic AMP response element, and HTF4/HEB in transcriptional regulation of the neurospecific,...
TY - JOUR. T1 - Interplay of the E box, the cyclic AMP response element, and HTF4/HEB in transcriptional regulation of the neurospecific, neurotrophin-inducible vgf gene. AU - Di Rocco, Giuliana. AU - Pennuto, Maria. AU - Illi, Barbara. AU - Canu, Nadia. AU - Filocamo, Gessica. AU - Trani, Eugenia. AU - Rinaldi, Anna Maria. AU - Possenti, Roberta. AU - Mandolesi, Georgia. AU - Sirinian, M. Isabella. AU - Jucker, Richard. AU - Levi, Andrea. AU - Nasi, Sergio. PY - 1997/3. Y1 - 1997/3. N2 - vgf is a neurotrophin response-specific, developmentally regulated gene that codes for a neurosecretory polypeptide. Its transcription in neuronal cells is selectively activated by the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor, and neurotrophin 3, which induce survival and differentiation, and not by epidermal growth factor. We studied a short region of the rat vgf promoter which is essential for its regulated expression. A cyclic AMP response element (CRE) within this region is ...
https://moh-it.pure.elsevier.com/en/publications/interplay-of-the-e-box-the-cyclic-amp-response-element-and-htf4he
The versatile role of MSKs in transcriptional regulationThe versatile role of MSKs in transcriptional regulation
article{955774, abstract = {Among the mitogen-activated protein kinase (MAPK) targets, MSKs (mitogen- and stress-activated protein kinases) comprise a particularly interesting protein family. Because MSKs can be activated by both extracellular-signal-regulated kinase and p38 MAPKs, they are activated by many physiological and pathological stimuli. About ten years after their original discovery, they have been recognized as versatile kinases regulating gene transcription at multiple levels. MSKs directly target transcription factors, such as cAMP-response-element-binding protein and nuclear factor-kappaB, thereby enhancing their transcriptional activity. They also induce histone phosphorylation, which is accompanied by chromatin relaxation and facilitated binding of additional regulatory proteins. Here, we review the current knowledge on MSK activation and its molecular targets, focusing on recent insights into the role of MSKs at multiple levels of transcriptional regulation.}, author = ...
https://biblio.ugent.be/publication/955774
Transcriptional and posttranslational plasticity and the generation of inflammatory pain | PNASTranscriptional and posttranslational plasticity and the generation of inflammatory pain | PNAS
In addition to these posttranslational changes, an alteration in the expression of effector molecules in the DRG (66) and dorsal horn (67-69) is a prominent feature of inflammation and can be initiated in two ways. The first is as a result of an activity-dependent activation of the CREB transcription factor both in DRG and dorsal horn neurons (ref. 70; Fig. 3). As discussed above, this will result, after a delay of several hours for transcription and translation in the DRG and protein transport to central terminals, in a potentiated system in which the C fibers are primed to exert a greater effect on dorsal horn neurons as a result of an increased expression of neuromodulators like BDNF. In addition, the dorsal horn is simultaneously made hyperresponsive to such neuromodulators as a result of an activity-dependent increased expression of the TrkB receptor. The second way that transcriptional changes occur after inflammation is via the production in the inflamed tissue of specific signal ...
https://www.pnas.org/content/96/14/7723?ijkey=829eb248b12f1bdfeb97f272ab201028b98fb7cc&keytype2=tf_ipsecsha
Delta-type opioid receptor - DrugBankDelta-type opioid receptor - DrugBank
negative regulation of gene expression / positive regulation of CREB transcription factor activity / G-protein coupled receptor signaling pathway / regulation of sensory perception of pain / protein import into nucleus, translocation / phospholipase C-activating G-protein coupled receptor signaling pathway / cellular response to growth factor stimulus / immune response / adult locomotory behavior / G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / neuropeptide signaling pathway / adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway / synaptic transmission / regulation of calcium ion transport / cellular response to toxic substance / eating behavior / cellular response to hypoxia / negative regulation of protein oligomerization / positive regulation of peptidyl-serine phosphorylation / regulation of mitochondrial membrane potential / opioid receptor signaling pathway ...
https://www.drugbank.ca/biodb/polypeptides/P41143
Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma |...Pulmonary microRNA profiles identify involvement of Creb1 and Sec14l3 in bronchial epithelial changes in allergic asthma |...
Asthma is highly prevalent, but current therapies cannot influence the chronic course of the disease. It is thus important to understand underlying early molecular events. In this study, we aimed to use microRNAs (miRNAs) - which are critical regulators of signaling cascades - to identify so far uncharacterized asthma pathogenesis pathways. Therefore, deregulation of miRNAs was assessed in whole lungs from mice with ovalbumin (OVA)-induced allergic airway inflammation (AAI). In silico predicted target genes were confirmed in reporter assays and in house-dust-mite (HDM) induced AAI and primary human bronchial epithelial cells (NHBE) cultured at the air-liquid interface. We identified and validated the transcription factor cAMP-responsive element binding protein (Creb1) and its transcriptional co-activators (Crtc1-3) as targets of miR-17, miR-144, and miR-21. Sec14-like 3 (Sec14l3) - a putative target of Creb1 - was down-regulated in both asthma models and in NHBE cells upon IL13 treatment, while its
https://www.nature.com/articles/srep46026?error=cookies_not_supported&code=d42cb076-2b24-4e21-b753-5d690660553b
Plus itPlus it
Extensive evidence implicates CREB-dependent gene transcription in memory (Bourtchuladze et al., 1994; Yin et al., 1994; Guzowski and McGaugh, 1997; Bartsch et al., 1998; Kida et al., 2002; Pittenger et al., 2002; Frankland et al., 2004). Multiple signaling pathways phosphorylate CREB at Ser133 (Shaywitz and Greenberg, 1999; Mayr and Montminy, 2001; Lonze and Ginty, 2002), which stimulates the recruitment of coactivators CBP/p300 (Chrivia et al., 1993; Parker et al., 1996). However, this phosphorylation event is not always sufficient to activate transcription (Impey et al., 1996; Mayr and Montminy, 2001) suggesting that CREB-mediated transcription is regulated by additional mechanisms.. In 2003, two laboratories identified a new family of CREB-specific coactivators, now referred to as CRTCs (Iourgenko et al., 2003; Conkright et al., 2003b). CRTC is thought to enhance transcription by facilitating the interaction of CREB with the RNA polymerase II pre-initiation complex (Conkright et al., 2003b; ...
http://www.jneurosci.org/content/32/49/17857
Epistatic interaction of CREB1 and KCNJ6 on rumination and negative emotionality<...
TY - JOUR. T1 - Epistatic interaction of CREB1 and KCNJ6 on rumination and negative emotionality. AU - Lazary, Judit. AU - Juhasz, Gabriella. AU - Anderson, Ian M.. AU - Jacob, Christian P.. AU - Nguyen, T. Trang. AU - Lesch, Klaus Peter. AU - Reif, Andreas. AU - Deakin, J. F.William. AU - Bagdy, Gyorgy. PY - 2011/1/1. Y1 - 2011/1/1. N2 - G protein-activated K+ channel 2 (GIRK2) and cAMP-response element binding protein (CREB1) are involved in synaptic plasticity and their genes have been implicated depression and memory processing. Excessive rumination is a core cognitive feature of depression which is also present in remission. High scores on the Ruminative Response Scale (RRS) questionnaire are predictive of relapse and recurrence. Since rumination involves memory, we tested the hypothesis that variation in the genes encoding GIRK2 (KCNJ6) and CREB1 mechanisms would influence RRS scores. GIRK2 and CREB1 polymorphisms were studied in two independent samples (n = 651 and n = 1174) from the ...
https://hungary.pure.elsevier.com/hu/publications/epistatic-interaction-of-creb1-and-kcnj6-on-rumination-and-negati
CREB in cognition - WikipediaCREB in cognition - Wikipedia
The cellular transcription factor CREB (cAMP response element-binding protein) helps learning and the stabilization and retrieval of fear-based, long-term memories. This is done mainly through its expression in the hippocampus and the amygdala. Studies supporting the role of CREB in cognition include those that knock out the gene, reduce its expression, or overexpress it. Research suggests that CREB has a role in the molecular steps that stabilize memory in the brain, including that of emotional memory. Evidence of CREBs role in emotional memory falls into three experimental categories: negative manipulations (where the levels of CREB were lowered), positive manipulations (where the levels of CREB were increased), and non-interventions (where the endogenous levels of CREB were tracked before and after learning). Knockout studies in Aplysia sea slugs indicated that decreasing CREB function blocks long-term changes in synaptic function, but not short-term ones. Changes in synaptic function (i.e., ...
https://en.wikipedia.org/wiki/CREB_in_cognition
Frontiers | Differences in the Flexibility of Switching Learning Strategies and CREB Phosphorylation Levels in Prefrontal...Frontiers | Differences in the Flexibility of Switching Learning Strategies and CREB Phosphorylation Levels in Prefrontal...
Flexibility in using different learning strategies was assessed in two different inbred strains of mice, the C57BL/6 and DBA/2 strains. Mice were trained sequentially in two different Morris water maze protocols that tested their ability to switch their learning strategy to complete a new task after first being trained in a different task. Training consisted either of visible platform trials (cued training) followed by subsequent hidden platform trials (place training) or the reverse sequence (place training followed by cued training). Both strains of mice showed equivalent performance in the type of training (cued or place) that they received first. However, C57BL/6 mice showed significantly better performances than DBA/2 mice following the switch in training protocols, irrespective of the order of training. After completion of the switched training session, levels of cAMP response element-binding protein (CREB) and phosphorylated CREB (pCREB) were measured in the hippocampus, striatum and prefrontal
https://www.frontiersin.org/articles/10.3389/fnbeh.2016.00176/full
OxytocinOxytocin
January 2012). "Antidepressant-like effect of sildenafil through oxytocin-dependent cyclic AMP response element-binding protein ... This precursor protein also includes the oxytocin carrier protein neurophysin I. The inactive precursor protein is ... Oxytocin modulates fear responses by enhancing the maintenance of social memories. Rats that are genetically modified to have a ... In the pituitary gland, oxytocin is packaged in large, dense-core vesicles, where it is bound to neurophysin I as shown in the ...
https://en.wikipedia.org/wiki/Oxytocin
CAMP responsive element modulatorCAMP responsive element modulator
Meyer TE, Habener JF (Nov 1992). "Cyclic AMP response element binding protein CREB and modulator protein CREM are products of ... Pongubala JM, Atchison ML (Apr 1995). "Activating transcription factor 1 and cyclic AMP response element modulator can modulate ... "The cyclic AMP response element modulator family regulates the insulin gene transcription by interacting with transcription ... "Modulation of Tax and PKA-mediated expression of HTLV-I promoter via cAMP response element binding and modulator proteins CREB ...
https://en.wikipedia.org/wiki/CAMP_responsive_element_modulator
RELARELA
Parry GC, Mackman N (Dec 1997). "Role of cyclic AMP response element-binding protein in cyclic AMP inhibition of NF-kappaB- ... "Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with ... Gerritsen ME, Williams AJ, Neish AS, Moore S, Shi Y, Collins T (Apr 1997). "CREB-binding protein/p300 are transcriptional ... Aarnisalo P, Palvimo JJ, Jänne OA (Mar 1998). "CREB-binding protein in androgen receptor-mediated signaling". Proceedings of ...
https://en.wikipedia.org/wiki/RELA
Adenovirus early region 1AAdenovirus early region 1A
The structure of cyclic-AMP response element binding protein (CBP) and E1A complex was determined by NMR. It reveals that CR1 ... Early proteins typically encode non-structural proteins that are necessary for replication, whereas late structural proteins ... "The structure of the site on adenovirus early region 1A responsible for binding to TATA-binding protein determined by NMR ... The fixed structure allows this domain to recognize TATA-binding protein (TBP) and activate transcription of certain genes. The ...
https://en.wikipedia.org/wiki/Adenovirus_early_region_1A
Activating transcription factor 2Activating transcription factor 2
Kara CJ, Liou HC, Ivashkiv LB, Glimcher LH (April 1990). "A cDNA for a human cyclic AMP response element-binding protein which ... Kara CJ, Liou HC, Ivashkiv LB, Glimcher LH (1990). "A cDNA for a human cyclic AMP response element-binding protein which is ... "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain". EMBO J. 8 (7): 2023-8. ... "Assignment of the gene for cyclic AMP-response element binding protein 2 (CREB2) to human chromosome 2q24.1-q32". Genomics. 11 ...
https://en.wikipedia.org/wiki/Activating_transcription_factor_2
CREBCREB
Drosophila Cyclic-AMP response element binding protein A - The Interactive Fly Drosophila Cyclic-AMP response element binding ... The protein also has a magnesium ion that facilitates binding to DNA. The cAMP response element (CRE) is the response element ... "Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene". Nature. 328 (6126): 175-178. Bibcode ... CREB-TF (CREB, cAMP response element-binding protein) is a cellular transcription factor. It binds to certain DNA sequences ...
https://en.wikipedia.org/wiki/CREB
ICAM-1ICAM-1
"ICAM-1-coupled signaling pathways in astrocytes converge to cyclic AMP response element-binding protein phosphorylation and TNF ... These three proteins are generally expressed on endothelial cells and leukocytes, and they bind to ICAM-1 to facilitate ... The presence of heavy glycosylation and other structural characteristics of ICAM-1 lend the protein binding sites for numerous ... ICAM-1 possesses binding sites for a number of immune-associated ligands. Notably, ICAM-1 binds to macrophage adhesion ligand-1 ...
https://en.wikipedia.org/wiki/ICAM-1
Lithium (medication)Lithium (medication)
GSK-3β inhibits the transcription factors β-catenin and cyclic AMP (cAMP) response element binding protein (CREB), by ... Lithium was found to increase the basal levels of cyclic AMP but impair receptor coupled stimulation of cyclic AMP production. ... It is hypothesized that the dual effects of lithium are due to the inhibition of G-proteins that mediate cyclic AMP production ... Over a long period of lithium treatment, cyclic AMP and adenylate cyclase levels are further changed by gene transcription ...
https://en.wikipedia.org/wiki/Lithium_(medication)
Transcription factor JunTranscription factor Jun
Kara CJ, Liou HC, Ivashkiv LB, Glimcher LH (April 1990). "A cDNA for a human cyclic AMP response element-binding protein which ... "Isolation and characterization of a novel member of the gene family encoding the cAMP response element-binding protein CRE-BP1 ... Venugopal R, Jaiswal AK (December 1998). "Nrf2 and Nrf1 in association with Jun proteins regulate antioxidant response element- ... forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later ...
https://en.wikipedia.org/wiki/Transcription_factor_Jun
Kaang Bong-kiunKaang Bong-kiun
Lee JA, Kim H, Lee YS, Kaang BK (2003). "Overexpression and RNA interference of Ap-cyclic AMP-response element binding protein- ... "AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic ... transcription requires the protein kinase-A-mediated phosphorylation of the cAMP-response element-binding protein (CREB). ... he found that multiple pulses of serotonin stimulate gene expression that is mediated by the cAMP-response element (CRE). He ...
https://en.wikipedia.org/wiki/Kaang_Bong-kiun
Nerve growth factorNerve growth factor
... act to phosphorylate the cyclic AMP response element binding protein (CREB) transcription factor. Phosphorylated CREB ... Survival and PCD mechanisms are mediated through adaptor protein binding to the death domain of the p75NTR cytoplasmic tail. ... This pathway begins with the Trk receptor complex-recruitment of a second adaptor protein called growth factor-receptor bound ... The active Ras protein phosphorylates several proteins, along with the serine/threonine kinase, Raf. Raf in turn activates the ...
https://en.wikipedia.org/wiki/Nerve_growth_factor
Neural backpropagationNeural backpropagation
... action potentials have been shown to induce BDNF-dependent phosphorylation of cyclic AMP response element-binding protein (CREB ... This change also affects future integration of signals, leading to at least a short-term response difference between the ... boosting synaptic responses, resetting membrane potential, retrograde actions at synapses and conditional axonal output. ...
https://en.wikipedia.org/wiki/Neural_backpropagation
Epigenetics of depressionEpigenetics of depression
Cyclic-AMP response element-binding protein (CREB), which is thought to be involved in GDNF regulation, associates with the ... cell line-derived neurotrophic factor expression through cAMP response element-binding protein activated by heat shock protein ... CREB Binding Protein. Therefore, this antidepressant, by increasing acetylation, works to lessen the HPA response, and as a ... It is thought that this is happening due to an interaction between the response element of GR and the acetyltransferase, ...
https://en.wikipedia.org/wiki/Epigenetics_of_depression
Barrel cortexBarrel cortex
... calmodulin-dependent protein kinase II (CaMKII) or cyclic-AMP response element binding protein (CREB). In these mice, ... Stimulation of multiple whiskers may produce a response that is equal to the sum of the responses if each whisker was ... In deprived cortex, neuronal responses to spared whiskers are enhanced and responses to deprived whiskers are weakened. These ... with the weakening of deprived response preceding the strengthening of spared response, implying that they have different ...
https://en.wikipedia.org/wiki/Barrel_cortex
List of acronyms: CList of acronyms: C
Cyclic AMP-response Element Binding (protein) CREEP - (a) Committee for the Re-Election of the President, a pejorative nickname ... Cyclic Code Shift Keying CCT - (i) Current Commitments Team CCTT - (i) Close Combat Tactical Trainer cd - (s) Candela Cd - (s) ... Computer Emergency Response Team ces - (s) Czech language (ISO 639-2 code) CESS - (i) Centre for Earth Science Studies (India) ... from the chemical symbols of the two component elements) CUW - (s) Curaçao (ISO 3166 trigram) cv - (s) Chuvash language (ISO ...
https://en.wikipedia.org/wiki/List_of_acronyms:_C
Molecular and epigenetic mechanisms of alcoholismMolecular and epigenetic mechanisms of alcoholism
Chronic ethanol administration decreases phosphorylation of cyclic AMP response element-binding protein in granule cells of rat ... exposure alters the phosphorylation of cyclic AMP responsive element binding protein and cyclic AMP responsive element binding ... Pandey, S.C. (2004). The gene transcription factor cyclic AMP responsive element binding protein: role in positive and negative ... cyclic monophosphate response element binding protein activity in an age- and brain region-specific manner. Alcohol Clin Exp ...
https://en.wikipedia.org/wiki/Molecular_and_epigenetic_mechanisms_of_alcoholism
Follicle-stimulating hormone receptorFollicle-stimulating hormone receptor
DNA in the cell nucleus binds to phosphorylated proteins through the cyclic AMP response element (CRE), which results in the ... Cyclic AMP-dependent protein kinases (protein kinase A) are activated by the signal chain coming from the G protein (that was ... via adenylate cyclase and cyclic AMP (cAMP). These protein kinases are present as tetramers with two regulatory units and two ... Upon binding of cAMP to the regulatory units, the catalytic units are released and initiate the phosphorylation of proteins, ...
https://en.wikipedia.org/wiki/Follicle-stimulating_hormone_receptor
CREB5CREB5
Cyclic AMP-responsive element-binding protein 5 is a protein that in humans is encoded by the CREB5 gene. The product of this ... cAMP response element)-binding protein family. Members of this family contain zinc finger and bZIP DNA-binding domains. The ... "Isolation and characterization of a novel member of the gene family encoding the cAMP response element-binding protein CRE-BP1 ... "Entrez Gene: CREB5 cAMP responsive element binding protein 5". Cumulated Index Medicus. U.S. Department of Health and Human ...
https://en.wikipedia.org/wiki/CREB5
Activity-regulated cytoskeleton-associated proteinActivity-regulated cytoskeleton-associated protein
7 kb upstream that contains binding sites for cyclic AMP response element-binding protein (CREB), myocyte enhancer factor 2 ( ... a serum response element (SRE; see serum response factor) at ~1.5 kb upstream of the initiation site. a second SRE at ~6.5 kb; ... The common factor for these signaling molecules involves activation of cyclic-AMP and its downstream target protein kinase A ( ... Additionally, the protein has binding sites for endophilin 3 and dynamin 2 at amino acids 89-100 and 195-214, respectively. ...
https://en.wikipedia.org/wiki/Activity-regulated_cytoskeleton-associated_protein
Alpha cellAlpha cell
... protein kinase A (PKA) is activated and phosphorylates the transcription factor cAMP Response Element Binding (CREB) protein. ... This ligand binding causes the activation of adenylate cyclase, which causes the creation of cyclic AMP (cAMP). As the ... Somatostatin inhibits glucagon secretion through the activation of SSTR2, a membrane bound protein that when activated causes a ... This protein channel allows zinc to cross the plasma membrane into the cell. When ZnT8 is under-expressed, there is a marked ...
https://en.wikipedia.org/wiki/Alpha_cell
PFKFB3PFKFB3
... and progesterone response element. Expression of the promoter is shown to be induce by phorbol esters and cyclic-AMP-dependent ... N-terminal structure that secures the binding of fructose-6-phosphate to the active site via interaction with the protein's '2- ... The promoter of PFKFB3 contains binding sites, called hypoxia response elements (HREs), that recruit the binding of hypoxia- ... The PFKFB3 promoter is predicted to contain multiple binding sites, including Sp-1 and AP-2 binding sites. It also contains ...
https://en.wikipedia.org/wiki/PFKFB3
NeurotensinNeurotensin
Specifically, estrogen increased cAMP activity and cAMP response element-binding protein phosphorylation in neuroblastoma cells ... modulated by estrogen in both human SK-N-SH neuroblastoma cell cultures as well as in mice through interactions with cyclic AMP ... Additionally, neurotensin gene transcription was blocked in knock-out mice lacking the RIIβ subunit of the protein kinase A ... Neurotensin is synthesized as part of a 169 or 170 amino acid precursor protein that also contains the related neuropeptide ...
https://en.wikipedia.org/wiki/Neurotensin
CREB1CREB1
Pandey SC (Oct 2004). "The gene transcription factor cyclic AMP-responsive element binding protein: role in positive and ... is a protein that in humans is encoded by the CREB1 gene. This protein binds the cAMP response element, a DNA nucleotide ... "Synergism between calcium and cyclic GMP in cyclic AMP response element-dependent transcriptional regulation requires ... Hoeffler JP, Meyer TE, Yun Y, Jameson JL, Habener JF (Dec 1988). "Cyclic AMP-responsive DNA-binding protein: structure based on ...
https://en.wikipedia.org/wiki/CREB1
Neuronal memory allocationNeuronal memory allocation
... cyclic AMP responsive element-binding protein) in this process. Certain synapses on recruited neurons are more likely to ... The transcription factor cAMP response element-binding protein (CREB) is a well-studied mechanism of neuronal memory allocation ... Nguyen, P. V., & Woo, N. H. (2003). Regulation of hippocampal synaptic plasticity by cyclic AMP-dependent protein kinases. ... For example, the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) pathways appear to participate in neuronal ...
https://en.wikipedia.org/wiki/Neuronal_memory_allocation
ATF1ATF1
Sun P, Lou L, Maurer RA (1996). "Regulation of activating transcription factor-1 and the cAMP response element-binding protein ... Pongubala JM, Atchison ML (1995). "Activating transcription factor 1 and cyclic AMP response element modulator can modulate the ... "The cAMP-regulated enhancer-binding protein ATF-1 activates transcription in response to cAMP-dependent protein kinase A." J. ... Cyclic AMP-dependent transcription factor ATF-1 is a protein that in humans is encoded by the ATF1 gene. This gene encodes an ...
https://en.wikipedia.org/wiki/ATF1
Insulin regulatory sequenceInsulin regulatory sequence
Lastly, several "cyclic AMP response elements" with sequence TGACGTCA that binds CREB. In humans, a "Z-element" resides 243-292 ... each of which serve as binding sites for distinct regulatory proteins. First, multiple A/T-rich sequences, called "A elements ... These regions are primarily bound by PDX-1, but also Cdx2 and Isl-1. Second, two so-called "C elements" - C1 located 107-118 ... C2 (also called the "pancreatic islet cell enhancer sequence" or "PISCES") is bound by PAX6. Third, an "E element" (two in ...
https://en.wikipedia.org/wiki/Insulin_regulatory_sequence
TAF4TAF4
Inada A, Someya Y, Yamada Y, Ihara Y, Kubota A, Ban N, Watanabe R, Tsuda K, Seino Y (1999). "The cyclic AMP response element ... CBX5m TATA binding protein, and Transcription initiation protein SPT3 homolog. Yeast TFIID comprises the TATA binding protein ... and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded ... TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated ...
https://en.wikipedia.org/wiki/TAF4
Hormone receptorHormone receptor
Their response elements are DNA sequences (promoters) that are bound by the complex of the steroid bound to its receptor. The ... These receptors generally function via intracellular second messengers, including cyclic AMP (cAMP), cyclic GMP (cGMP), ... The features of G proteins include GDP/GTP binding, GTP hydrolysis and guanosine nucleotide exchange. When a ligand binds to a ... Hormone receptor proteins bind to a hormone as a result of an accumulation of weak interactions. Because of the relatively ...
https://en.wikipedia.org/wiki/Hormone_receptor
Robert Clarke (academic)Robert Clarke (academic)
... and cyclic AMP response element binding with acquired resistance to Faslodex (ICI 182,780)". Cancer Research. 62 (12): 3428- ... He was among the pioneers to implicate the unfolded protein response (UPR) in acquired endocrine resistance and in regulating ... the Unfolded Protein Response, Autophagy, and the Integrated Regulation of Breast Cancer Cell Fate". Cancer Research. 72 (6): ... "Autophagy and unfolded protein response (UPR) regulate mammary gland involution by restraining apoptosis-driven irreversible ...
https://en.wikipedia.org/wiki/Robert_Clarke_(academic)
CEBPBCEBPB
"Synergism between calcium and cyclic GMP in cyclic AMP response element-dependent transcriptional regulation requires ... CCAAT/enhancer-binding protein beta is a protein that in humans is encoded by the CEBPB gene. The protein encoded by this ... In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I ... Hanlon M, Sealy L (May 1999). "Ras regulates the association of serum response factor and CCAAT/enhancer-binding protein beta ...
https://en.wikipedia.org/wiki/CEBPB
Olfactory receptor neuronOlfactory receptor neuron
The activated OR in turn activates the intracellular G-protein, GOLF (GNAL), adenylate cyclase and production of cyclic AMP ( ... Calcium first binds to calmodulin to form CaM. CaM will then bind to the CNG channel and close it, stopping the sodium and ... The effect of this negative feedback response inhibits the neuron from further activation when another odor molecule is ... AC with 0 elements, Chemoreceptor cells, Olfactory system, Neurons, Signal transduction). ...
https://en.wikipedia.org/wiki/Olfactory_receptor_neuron
Transduction (physiology)Transduction (physiology)
The G-protein activates a downstream signalling cascade that causes increased level of cyclic-AMP (cAMP), which trigger ... In the olfactory system, odorant molecules in the mucus bind to G-protein receptors on olfactory cells. ... Because of the change, a change in light intensity causes the response of the rods to be much slower than expected (for a ... Koike, Takuji; Wada, Hiroshi; Kobayashi, Toshimitsu (2002). "Modeling of the human middle ear using the finite-element method ...
https://en.wikipedia.org/wiki/Transduction_(physiology)
RiboswitchRiboswitch
... "cyclic di-GMP" below). cyclic di-AMP riboswitches (also called ydaO/yuaA) bind the signaling molecule cyclic di-AMP. cyclic di- ... including protein-free binding assays, and metabolite-binding riboswitches were established as a new mechanism of gene ... The aptamer directly binds the small molecule, and the expression platform undergoes structural changes in response to the ... Cobalamin riboswitch (also B12-element), which binds either adenosylcobalamin (the coenzyme form of vitamin B12) or ...
https://en.wikipedia.org/wiki/Riboswitch
G beta-gamma complexG beta-gamma complex
... and is a component of heterotrimeric G proteins. Heterotrimeric G proteins, also called guanosine nucleotide-binding proteins, ... or inhibiting adenylyl cyclase leading to the intracellular increase or decrease of the secondary messenger cyclic AMP. For ... the Gβγ complex associated with a mating factor receptor-coupled G protein in yeast was found to initiate a pheromone response ... The Gβγ complex is an essential element in the GPCR signaling cascade. It has two main states for which it performs different ...
https://en.wikipedia.org/wiki/G_beta-gamma_complex
HormoneHormone
... cyclic AMP). Some protein hormones also interact with intracellular receptors located in the cytoplasm or nucleus by an ... Most hormones initiate a cellular response by initially binding to either cell membrane associated or intracellular receptors. ... a crucial element in regulating the metabolic rate. Autocrine signaling Adipokine Cytokine Hepatokine Endocrine disease ... An example of the usage of hormone-binding proteins is in the thyroxine-binding protein which carries up to 80% of all ...
https://en.wikipedia.org/wiki/Hormone
Picornain 3CPicornain 3C
Also, 3C and 2A are responsible for down-regulation of cyclic AMP responsive element binding protein (CREB), a cellular ... antiviral response. Cys24Ser (C24S) is a homolog of Hepatitis 3C proteinase, is responsible for inactivating Cys172 through ... binding protein, PABP. This disruption of this binding domain results in down regulation of the initiation of cap dependent ... P1 encodes for a proteins (VP1, VP2, VP3, and VP4) that make the capsid proteins. P2 and P3 proteins assist in infectivity of ...
https://en.wikipedia.org/wiki/Picornain_3C
Bordetella pertussisBordetella pertussis
... inhibits G protein coupling that regulates an adenylate cyclase-mediated conversion of ATP to cyclic AMP. The end result is ... The bacterium contains a surface protein, filamentous haemagglutinin adhesin, which binds to the sulfatides found on cilia of ... This is because B. pertussis inhibits the immune response, so very little mucus is generated in the lungs. A prolonged cough ... The primers used for PCR usually target the transposable elements IS481 and IS1001. Several diagnostic tests are available, ...
https://en.wikipedia.org/wiki/Bordetella_pertussis
Microcystin-LRMicrocystin-LR
... but has no effect on protein kinase C or cyclic AMP-dependent kinase. Mutagenicity does not appear to occur for purified toxins ... When microcystin-LR binds directly to the catalytic center of the PPP enzymes, they block the access of the substrate to the ... These two variable elements are always standard L-amino acids. In microcystin-LR these are leucine and arginine. more than 250 ... There is no change in the expression of selected genes involved in the cellular response to DNA damage after a 4-hour exposure ...
https://en.wikipedia.org/wiki/Microcystin-LR
Biochemical cascadeBiochemical cascade
Then, it converts adenosine triphosphate into cyclic AMP, which activates Protein kinase A. PKA leads to protein tyrosine ... growth hormone response element) IGF-1 and IGFBP-3 expression Via THR/THRE (thyroid hormone response element) Angiotensinogen ... One example of a protein that binds to adaptor proteins and become activated is PLC that is very important in the lymphocyte ... "Cyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte". Development. 136 (11): ...
https://en.wikipedia.org/wiki/Biochemical_cascade
Prostaglandin DP2 receptorProstaglandin DP2 receptor
... and adenyl cyclase converts AMP into cyclic AMP (cAMP) thereby down-regulating cAMP-responsive proteins involved in cell ... G protein-coupled receptors (GPCRs) such as DP2 are integral membrane proteins that, when bound by their cognate ligands (or, ... Venet F, Lepape A, Debard AL, Bienvenu J, Bohé J, Monneret G (December 2004). "The Th2 response as monitored by CRTH2 or CCR3 ... DP2 activation also stimulates eosinophils and basophils to release the many pro-allergic elements of their granules to the ...
https://en.wikipedia.org/wiki/Prostaglandin_DP2_receptor
Cannabinoid receptorCannabinoid receptor
September 2011). "Modeling of ligand binding to G protein coupled receptors: cannabinoid CB1, CB2 and adrenergic β 2 AR". ... and thereby the production of the second messenger molecule cyclic AMP), and positively influenced inwardly rectifying ... Similar responses are produced when introduced in alternative methods, only in a more concentrated form than what is naturally ... and certain neuronal elements of the peripheral or central nervous systems (2011). The existence of additional cannabinoid ...
https://en.wikipedia.org/wiki/Cannabinoid_receptor
Signal transductionSignal transduction
... most commonly protein phosphorylation catalyzed by protein kinases, which ultimately results in a cellular response. Proteins ... Some of them create second messengers such as cyclic AMP and IP3, the latter controlling the release of intracellular calcium ... Many adaptor proteins and enzymes activated as part of signal transduction possess specialized protein domains that bind to ... has been shown to be a critical element in the plant immune response to signal molecules from bacterial pathogens and plant ...
https://en.wikipedia.org/wiki/Signal_transduction
YWHAZYWHAZ
... though it can also bind phosphothreonine proteins and unphosphorylated proteins. By extension, 14-3-3 proteins are involved in ... Chow CW, Davis RJ (January 2000). "Integration of calcium and cyclic AMP signaling pathways by 14-3-3". Molecular and Cellular ... Surfactant is an important element in the maintenance of lung and respiratory functions. A lack of surfactant is closely ... Furthermore, 14-3-3ζ may regulate glucose receptor trafficking in response to insulin levels through its interaction with IRS1 ...
https://en.wikipedia.org/wiki/YWHAZ
Cell migrationCell migration
... amoeba Dictyostelium discoideum is useful to researchers because they consistently exhibit chemotaxis in response to cyclic AMP ... For a cell to move, it is necessary to bring a fresh supply of "feet" (proteins called integrins, which attach a cell to the ... In addition, cytoskeletal elements are able to interact extensively and intimately with a cell's plasma membrane. Other ... aggregates of a surface molecule are cross-linked with a fluorescent antibody or when small beads become artificially bound to ...
https://en.wikipedia.org/wiki/Cell_migration
Prostaglandin receptorProstaglandin receptor
... p38 mitogen-activated protein kinases (p38 Mpk), and cAMP response element-binding protein (CREB) which when activated ... production of cyclic AMP [cAMP]); e) G proteins types to which they link and activate, i.e. those containing the Gs alpha ... which binds and responds to PGH2 and TXA2 equally well. All of the prostanoid receptors are G protein-coupled receptors ... They are named based on the prostanoid to which they preferentially bind and respond, e.g. the receptor responsive to PGI2 at ...
https://en.wikipedia.org/wiki/Prostaglandin_receptor
NetrinNetrin
DCC and UNC-5 proteins mediate netrin-1 responses. The UNC-5 protein is mainly involved in signaling repulsion. DCC, which is ... "Cyclic AMP/GMP-dependent modulation of Ca2+ channels sets the polarity of nerve growth-cone turning". Nature. 423 (6943): 990-5 ... Netrins 1, 3, and 4 are secreted proteins, whereas G1 and G2 are membrane bound proteins tethered by Glycophosphatidylinositol ... and integral proteins α6β4 and α3β1. These elements in the extracellular matrix are responsible for epithelial cell adhesion ...
https://en.wikipedia.org/wiki/Netrin
SecretinSecretin
As secretin binds to these receptors, it stimulates adenylate cyclase activity and converts ATP to cyclic AMP. Cyclic AMP acts ... Secretin is released into circulation and/or intestinal lumen in response to low duodenal pH that ranges between 2 and 4.5 ... Also, the secretion of secretin is increased by the products of protein digestion bathing the mucosa of the upper small ... and the E-box element". Molecular Endocrinology. 18 (7): 1740-55. doi:10.1210/me.2003-0461. PMID 15118068. Lu Y, Owyang C (2009 ...
https://en.wikipedia.org/wiki/Secretin
Protein kinase AProtein kinase A
In protein synthesis, PKA first directly activates CREB, which binds the cAMP response element (CRE), altering the ... AMP-activated protein kinase). Protein kinase A, more precisely known as adenosine 3',5'-monophosphate (cyclic AMP)-dependent ... and a domain to bind the regulatory subunit. The regulatory subunit has domains to bind to cyclic AMP, a domain that interacts ... In cell biology, protein kinase A (PKA) is a family of enzymes whose activity is dependent on cellular levels of cyclic AMP ( ...
https://en.wikipedia.org/wiki/Protein_kinase_A
Phosphofructokinase 2Phosphofructokinase 2
... glucagon activates a cyclic AMP pathway; this results in Protein Kinase A, Protein Kinase C, or AMP-activated Protein Kinase ... Each variant differs in their primary tissue of expression, response to protein kinase regulation, and ratio of kinase/ ... The PFK-2 domain appears to be closely related to the superfamily of mononucleotide binding proteins including adenylate ... "An inducible gene product for 6-phosphofructo-2-kinase with an AU-rich instability element: role in tumor cell glycolysis and ...
https://en.wikipedia.org/wiki/Phosphofructokinase_2
Regulation of Puralpha gene transcription: evidence for autoregulation of Puralpha promoterRegulation of Puralpha gene transcription: evidence for autoregulation of Puralpha promoter
... has recently received special attention as this protein, by associating with the specific nucleotide sequence (GGN repeats) and ... several important cellular and viral proteins regulates crucial biological events such as transcription, repli … ... The single-stranded DNA and RNA binding protein, Puralpha, ... Cyclic AMP Response Element-Binding Protein * DNA-Binding ... The single-stranded DNA and RNA binding protein, Puralpha, has recently received special attention as this protein, by ...
https://pubmed.ncbi.nlm.nih.gov/11169980/?dopt=Abstract
Activation of cannabinoid receptor type 1 (Cb1r) disrupts hepatic insulin receptor signaling via cyclic AMP-response element...
... disrupts hepatic insulin receptor signaling via cyclic AMP-response element-binding protein H (Crebh)-mediated induction of ... disrupts hepatic insulin receptor signaling via cyclic AMP-response element-binding protein H (Crebh)-mediated induction of ... disrupts hepatic insulin receptor signaling via cyclic AMP-response element-binding protein H (Crebh)-mediated induction of ... disrupts hepatic insulin receptor signaling via cyclic AMP-response element-binding protein H (Crebh)-mediated induction of ...
https://koreauniv.pure.elsevier.com/en/publications/activation-of-cannabinoid-receptor-type-1-cb1r-disrupts-hepatic-i
العربيةالعربية
Cyclic AMP Response Element-Binding Protein. EN. dc.subject. Gene Deletion. EN. ... We examined the deletion of the survival motor neuron [‏SMN]‏ and neuronal apoptosis inhibitory protein [‏NAIP]‏ genes in ...
https://extranet.who.int/iris/restricted/handle/10665/118823?locale-attribute=ar&show=full
DeCS 2006 - Changed termsDeCS 2006 - Changed terms
DNA-Binding Protein, Cyclic AMP-Responsive. Cyclic AMP Response Element-Binding Protein. ... Proto-Oncogene Protein c-met. Proto-Oncogene Proteins c-met. Proto-Oncogene Protein p21(ras). Proto-Oncogene Proteins p21(ras) ... Proto-Oncogene Protein pp60(c-src). Proto-Oncogene Proteins pp60(c-src). ... Proto-Oncogene Protein c-kit. Proto-Oncogene Proteins c-kit. ... Heat-Shock Proteins 90. HSP90 Heat-Shock Proteins. I-kappa B. I ...
https://decs.bvs.br/I/rep_i2006.htm
CREBH mediates metabolic inflammation to hepatic VLDL overproduction and hyperlipoproteinemia<...
Cyclic AMP Response Element-Binding Protein/genetics, Diet, High-Fat, Hyperlipoproteinemias/metabolism, Inflammation/metabolism ... The current study established that cAMP-responsive-element-binding protein H (CREBH), an acute-phase transcription factor, ... The current study established that cAMP-responsive-element-binding protein H (CREBH), an acute-phase transcription factor, ... The current study established that cAMP-responsive-element-binding protein H (CREBH), an acute-phase transcription factor, ...
https://pure.qub.ac.uk/en/publications/crebh-mediates-metabolic-inflammation-to-hepatic-vldl-overproduct
Cells | Free Full-Text | Nerve, Muscle, and SynaptogenesisCells | Free Full-Text | Nerve, Muscle, and Synaptogenesis
... this model has been modified in response to work showing that postsynaptic differentiation can develop in the absence of ... regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein ... bone morphogenic protein 4 [175,176], and, through the intermediation of their dedicated binding proteins, insulin growth ... Insulin-like growth factor binding protein-2 binding to extracellular matrix plays a critical role in neuroblastoma cell ...
https://www.mdpi.com/2073-4409/8/11/1448
PDF] Protective effects of a green tea polyphenol, epigallocatechin-3-gallate, against sevoflurane-induced neuronal apoptosis...PDF] Protective effects of a green tea polyphenol, epigallocatechin-3-gallate, against sevoflurane-induced neuronal apoptosis...
Brain-derived neurotrophic factor (BDNF) and cyclic AMP response element-binding protein (CREB) are crucial for neurogenesis ... Brain-derived neurotrophic factor (BDNF) and cyclic AMP response element-binding protein (CREB) are crucial for neurogenesis ... inhibitor rolipram on behavior depend on cyclic AMP-response element binding protein (CREB)-mediated neurogenesis in the ... Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling ...
https://www.semanticscholar.org/paper/Protective-effects-of-a-green-tea-polyphenol%2C-of-in-Ding-Ma/58b83064eb0a0606b456240aba936c8b912ab453?p2df
Salicylic acid and aspirin inhibit the activity of RSK2 kinase and repress RSK2-dependent transcription of cyclic AMP response...Salicylic acid and aspirin inhibit the activity of RSK2 kinase and repress RSK2-dependent transcription of cyclic AMP response...
Salicylic acid and aspirin inhibit the activity of RSK2 kinase and repress RSK2-dependent transcription of cyclic AMP response ... element binding protein- and NF-kappa B-responsive genes. ... transcription of cyclic AMP response element binding protein- ... inhibit the activity of RSK2 kinase and repress RSK2-dependent transcription of cyclic AMP response element binding protein- ... and of RSK2 in mammalian cells and suppressed the phosphorylation of RSK2 substrates cAMP response element binding protein ( ...
https://go.drugbank.com/articles/A18155
The role of PI3Kγ in the immune system: new insights and translational implications | Nature Reviews ImmunologyThe role of PI3Kγ in the immune system: new insights and translational implications | Nature Reviews Immunology
Recent advances in protein structure determination and drug development have allowed for generation of highly specific PI3Kγ ... cyclic AMP-response element binding protein)/YAP (yes-associated protein) signaling. Arterioscler. Thromb. Vasc. Biol. 39, e91- ... Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha. ... A class of highly selective inhibitors bind to an active state of PI3Kgamma. Nat. Chem. Biol. 15, 348-357 (2019). ...
https://www.nature.com/articles/s41577-022-00701-8?error=cookies_not_supported&code=4987ecc9-1ccb-4768-a869-d89b279df0f4
Publications | National Institute on Alcohol Abuse and Alcoholism | Update on the Genetics of AlcoholismPublications | National Institute on Alcohol Abuse and Alcoholism | Update on the Genetics of Alcoholism
Chronic ethanol administration decreases phosphorylation of cyclic AMP response element binding protein in granule cells of rat ... Pandey, S.C. The gene transcription factor cyclic AMP-responsive element binding protein: Role in positive and negative ... Pandey, S.C.; Roy, A; Zhang, H.; and Xu, T. Partial deletion of the cAMP response element-binding protein gene promotes alcohol ... acetylation and thus chromatin remodeling may be regulated involves a protein called cyclic-AMP responsive-element binding ( ...
https://pubs.niaaa.nih.gov/publications/arcr343/293-305.htm
Plus itPlus it
... influx via L-type calcium channels activates the SRC/ERK/cyclic-AMP response element binding protein signal pathway and BCL-2 ... leading to opposing influences on cAMP response element-binding protein. J Neurosci 25:5066-5078. ... and E2 may affect posttranslational modifications and protein-protein interactions to modulate channel function (Davare and ... 2001) Cyclic changes in estradiol regulate synaptic plasticity through the MAP kinase pathway. Proc Natl Acad Sci U S A 98: ...
https://www.jneurosci.org/content/29/19/6058
ZFIN Publication: Dworkin et al., 2007ZFIN Publication: Dworkin et al., 2007
Cyclic AMP Response Element-Binding Protein/physiology*. *Mesencephalon/physiology*. *Neurons/cytology*. *Phosphorylation ... The cAMP Response Element-Binding protein (CREB) is a transcription factor important for neuronal survival, differentiation and ...
https://zfin.org/ZDB-PUB-070614-18
Isotype: Rabbit-IgG, Positive Tested Species Reactivity: Drosophila, SheepIsotype: Rabbit-IgG, Positive Tested Species Reactivity: Drosophila, Sheep
Cyclic AMP response element-binding protein A; CrebA. $45.00 Even-skipped. Segmentation protein even-skipped ...
https://dshb.biology.uiowa.edu/custitem16/Rabbit-IgG/custitem34/Drosophila,Sheep
Antineuroinflammatory effects of dl-3-n-butylphthalide conferred by stimulation of Foxp3 and Ki-67 in an ischemic stroke model ...Antineuroinflammatory effects of dl-3-n-butylphthalide conferred by stimulation of Foxp3 and Ki-67 in an ischemic stroke model ...
Cyclic-AMP response element-binding protein (CREB) is a protein that regulates gene transcription and is involved in synaptic ... Activation of cAMP-response-element-binding protein (CREB) after focal cerebral ischemia stimulates neurogenesis in the adult ... significantly decreased the expression levels of both BDNF and cAMP/PKA/phosphorylated cAMP response element-binding protein ( ... Several metal-labeled antibodies bind specific proteins on a tissue slice, and then the tissue is ablated by a laser to produce ...
https://www.researchsquare.com/article/rs-22724/v1
Motor end plate innervation loss in diabetes and the role of insulin
... cyclic AMP response element binding protein,and glycogen synthase kinase 3beta but did not alter extracellular signal-regulated ... kinase, mitogen-activated protein kinase/extracellular signal-regulated kinase, or c-Jun amino-terminal kinase. Thus, direct ...
https://www.healthpartners.com/knowledgeexchange/display/document-rn19523
Mary Stevenson | Harvard Catalyst Profiles | Harvard CatalystMary Stevenson | Harvard Catalyst Profiles | Harvard Catalyst
... inhibit the activity of RSK2 kinase and repress RSK2-dependent transcription of cyclic AMP response element binding protein- ... Cyclic AMP and the heat shock response in Chinese hamster ovary cells. Biochem Biophys Res Commun. 1985 Jan 31; 126(2):911-6. ... Members of the 70-kilodalton heat shock protein family contain a highly conserved calmodulin-binding domain. Mol Cell Biol. ... Expression of heat shock proteins and heat shock protein messenger ribonucleic acid in human prostate carcinoma in vitro and in ...
https://connects.catalyst.harvard.edu/Profiles/display/Person/34376
Faculty Profile - School of Pharmacy and Pharmaceutical Sciences - University at BuffaloFaculty Profile - School of Pharmacy and Pharmaceutical Sciences - University at Buffalo
... effects of the phosphodiesterase-4 inhibitor rolipram on behavior depend on cyclic AMP response element binding protein- ... Ontogeny of rolipram-sensitive, low-K(m), cyclic AMP-specific phosphodiesterase in rat brain. Brain Res Dev Brain Res. 1999; ... Inhibition of cyclic AMP phosphodiesterase (PDE4) reverses memory deficits associated with NMDA receptor antagonism. ... Development of rolipram-sensitive, cyclic AMP phosphodiesterase (PDE4) in rat primary neuronal cultures. Brain Res Dev Brain ...
https://pharmacy.buffalo.edu/content/pharmacy/faculty-staff/faculty-profile.html?ubit=jod
Neuronal cell-based high-throughput screen for enhancers of mitochondrial function reveals luteolin as a modulator of...Neuronal cell-based high-throughput screen for enhancers of mitochondrial function reveals luteolin as a modulator of...
... activities in primary neurons and Caenorhabditis elegans expressing an expanded polyglutamine tract of the huntingtin protein. ... Following dose response curve-dependent selection, we identified the flavonoid luteolin as a primary hit. Further validation in ... Cyclic AMP response element-binding protein. CRC:. Concentration response curve. ER:. Endoplasmic reticulum ... The neuronal-specific protein NeuN, the presynaptic protein synaptophysin, and postsynaptic density protein 95 (PSD95) showed ...
https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-021-00979-5
麻醉學科 - 研究成果 - 臺北醫學大學
Cyclic AMP Response Element-Binding Protein 19% 24 引文 斯高帕斯（Scopus） ... Vasopressin inhibits endotoxin binding in activated macrophages. Chang, Y. Y., Yang, C. H., Wang, S. C., Kao, M. C., Tsai, P. S ... activator protein-1 and promotes cell mineralisation. Liao, M. H., Tai, Y-T., Cherng, Y-G., Liu, S. H., Chang, Y. A., Lin, P. I ... Genistein induces oestrogen receptor-α gene expression in osteoblasts through the activation of mitogen-activated protein ...
https://tmu.pure.elsevier.com/zh/organisations/department-of-anesthesiology-2/publications/?ordering=publicationYearThenTitle&descending=true&page=6
Clonidine-induced Neuronal Activation in the Spinal Cord Is Altered after Peripheral Nerve Injury | Anesthesiology | American...Clonidine-induced Neuronal Activation in the Spinal Cord Is Altered after Peripheral Nerve Injury | Anesthesiology | American...
Ma W, Quirion R: Increased phosphorylation of cyclic AMP response element-binding protein (CREB) in the superficial dorsal horn ... cAMP response element binding protein (pCREB). We chose this marker because of its importance in regulation of many proteins ... 1. Phosphorylated cAMP response element protein (pCREB) immunostaining in dorsal horn of normal and partial sciatic nerve- ... 1. Phosphorylated cAMP response element protein (pCREB) immunostaining in dorsal horn of normal and partial sciatic nerve- ...
https://pubs.asahq.org/anesthesiology/article/98/3/748/40686/Clonidine-induced-Neuronal-Activation-in-the
Gotu Kola (Seen on TV for Cellulite & Veins) - Best Price NutritionGotu Kola (Seen on TV for Cellulite & Veins) - Best Price Nutrition
... extract enhances phosphorylation of cyclic AMP response element binding protein in neuroblastoma cells expressing amyloid beta ... Protein. You will find the best prices and fastest shipping worldwide on your Supplement needs from our warehouse store. ...
https://www.bestpricenutrition.com/collections/supplements-seen-on-tv/products/solaray-gotu-kola-450mg-100-caps-1
Plus itPlus it
Role of the cyclic AMP response element binding complex and activation of mitogen-activated protein kinases in synergistic ... the cyclic adenosine monophosphate response element-binding protein, b) serum response element, c) nuclear factor of activated ... the cyclic adenosine monophosphate response element-binding protein, b) serum response element, c) nuclear factor of activated ... Activator protein (AP)‐1, cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB), nuclear factor of ...
https://erj.ersjournals.com/content/21/5/759
Faculty of Education - Research output - Okayama University
Antidepressant-like effect of sildenafil through oxytocin-dependent cyclic AMP response element-binding protein phosphorylation ... An initial estimator for models with scale parameter when binary response data are observed. Hirai, Y., 1999, In: 岡山大学教育学部研究収録. ... A protein transduction method using oligo-arginine (3R) for the delivery of transcription factors into cell nuclei. Hitsuda, T. ...
https://okayama.pure.elsevier.com/en/organisations/faculty-of-education/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle&ordering=title&descending=true&page=68
Critical Windows of Development Timeline - The Endocrine Disruption ExchangeCritical Windows of Development Timeline - The Endocrine Disruption Exchange
... dioxin suppresses contextual fear conditioning-accompanied activation of cyclic AMP response element-binding protein in the ... Prenatal exposure to bisphenol A up-regulates immune responses, including T helper 1 and T helper 2 responses, in mice. ... A dose-response analysis of the reproductive effects of a single gestational dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin in ... A dose-response study following in utero and lactational exposure to di-(2-ethylhexyl)-phthalate (DEHP): Non-monotonic dose- ...
http://endocrinedisruption.com/interactive-tools/critical-windows-of-development/scientific-references
An Effective Epigenetic-PARP Inhibitor Combination Therapy for Breast and Ovarian Cancers Independent of BRCA Mutations |...An Effective Epigenetic-PARP Inhibitor Combination Therapy for Breast and Ovarian Cancers Independent of BRCA Mutations |...
... with reduction of type I cyclic AMP-dependent protein kinase and cyclic AMP response element-binding protein DNA-binding ... Plasmids expressing Maltose Binding Protein (MBP)-tagged BRCA2 and KD BRCA2 constructs have been previously described (28, 29 ... Reversal of resistance to adriamycin by 8-chloro-cyclic AMP in adriamycin-resistant HL-60 leukemia cells is associated ... aiding in the PARPi response. This may explain why RG108 was not able to increase cell response to PARPi. In addition, ROS are ...
https://aacrjournals.org/clincancerres/article/24/13/3163/80952/An-Effective-Epigenetic-PARP-Inhibitor-Combination
Find Research outputs - Augusta University Research Profiles
Oligodendroglial cyclic AMP response element‐binding protein: A member of the CREB family of transcription factors. Sato‐Bigbee ... is an ATPase containing multiple members of a nucleotide-binding protein family. DeMartino, G. N., Moomaw, C. R., Zagnitko, O. ... PA28 activator protein forms regulatory caps on proteasome stacked rings. Gray, C. W., Slaughter, C. A. & DeMartino, G. N., Feb ... Multiple Otx binding sites required for expression of the Strongylocentrotus purpuratus Spec2a gene. Mao, C. A. N., Gan, L. & ...
https://augusta.pure.elsevier.com/en/publications/?showAdvanced=false&allConcepts=true&inferConcepts=true&publicationYear=2011&publicationYear=2013&publicationYear=2014&publicationYear=2015&publicationYear=2016&publicationYear=2017&publicationYear=2018&publicationYear=2019&publicationYear=2020&format=&nofollow=true&ordering=publicationYearThenTitle&descending=true&page=895
bioassay - Usa-China BIO
Human Cyclic AMP response element binding protein,CREB ELISA Kit. 634€. Human Cyclic AMP-dependent transcription factor ATF-2, ... Human Cyclic AMP-dependent transcription factor ATF-6,ATF-6 ELISA Kit. 634€ ...
http://usachinabio.com/catalogue/178339-bioassay?page=23
Witzig, T. E.<...
Cyclic AMP Response Element-Binding Protein 44% * Neoplasms 33% * Signal transduction inhibitor therapy for Lymphoma. Witzig, T ...
https://mayoclinic.pure.elsevier.com/en/persons/thomas-elmer-witzig
CREBKinaseKinasesPhosphorylationTranscription factorCell ProliferationMRNA and protein expressionPCREBGenesIschemiaNeuronsNucleotideReceptorAcoustic startle responseStimuliCAMPPathwaysCarbohydratesTargetsMiceAminoTranscriptionalCellsMechanismsExpressionMolecularActivationEnhancesLevelsSitesDevelopment
CREB17
  • Brain-derived neurotrophic factor (BDNF) and cyclic AMP response element-binding protein (CREB) are crucial for neurogenesis and synaptic plasticity. (semanticscholar.org)
  • NSAIDs inhibited the activity of purified RSK2 kinase in vitro and of RSK2 in mammalian cells and suppressed the phosphorylation of RSK2 substrates cAMP response element binding protein (CREB) and I-kappa B alpha in vivo. (drugbank.com)
  • The cAMP Response Element-Binding protein (CREB) is a transcription factor important for neuronal survival, differentiation and plasticity. (zfin.org)
  • Activator protein (AP)‐1, cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB), nuclear factor of activated T‐cells (NFAT), nuclear factor (NF)‐κB, the serum response element (SRE) complex and signal transducer and activator of transcription (STAT) are all known to be involved in proliferation and/or inflammation and have been implicated in the inflammatory state causing asthma and related airway diseases 8 . (ersjournals.com)
  • cAMP-response element binding protein (CREB) is a nuclear transcription factor activated by multiple extracellular signals including growth factors and hormones. (elsevier.com)
  • These extracellular cues activate CREB through phosphorylation at Ser133 by various protein serine/threonine kinases. (elsevier.com)
  • Once phosphorylated, it promotes its association with transcription coactivators CREB-binding protein (CBP) and its paralog p300 to activate CREB-dependent gene transcription. (elsevier.com)
  • We also investigated the effects of acupuncture stimulation at GV20 on the cholinergic system as well as the expression of brain-derived neurotrophic factor (BDNF) and cAMP-response element-binding protein (CREB) in the hippocampus. (biomedcentral.com)
  • Acupuncture stimulation at GV20 significantly alleviated memory-associated decreases in the levels of choline acetyltransferase (ChAT), BDNF and CREB proteins in the hippocampus. (biomedcentral.com)
  • Phosphorylation of CREB to pCREB (phosphorylated cyclic AMP response element binding protein) is increased after predator stress in fear circuitry, including in the right lateral column of the PAG (periaqueductal gray). (mun.ca)
  • Increasing CREB expression in right PAG was anxiogenic in the elevated plus maze, had no effect on risk assessment, and increased acoustic startle response while delaying startle habituation. (mun.ca)
  • The AP-1 activity binds efficiently to both AP-1 and activating transcription factor (ATF)/cAMP response element binding protein (CREB)-binding sites present in E1A-inducible promoters and presumably plays a role in the transcriptional activation of adenovirus genes by E1A proteins and cAMP. (princeton.edu)
  • Signalling pathway inhibitors were used to examine the roles of Rho, PKA, the cAMP response element binding protein (CREB) and NF-κB on the PGE 2 stimulated up-regulation of Nurr1. (arizona.edu)
  • Using primary cultures of ovarian granulosa cells from gonadotropin-primed immature rats, we have recently discovered that pituitary FSH and ovarian cytokine transforming growth factor beta 1 (TGFβ1) induce calcineurin-mediated dephosphorylation-activation of cAMP-response element-binding protein (CREB)-regulated transcription coactivator (CRTC2) to modulate the expression of Star, Cyp11a1, and Hsd3b leading to increased production of progesterone. (nycu.edu.tw)
  • It is known that the ovary-specific Cyp19a1 PII-promoter contains crucial response elements for CREB and nuclear receptor NR5A subfamily liver receptor homolog 1 (LRH1/NR5A2) and steroidogenic factor 1 (SF1/NR5A1), and that the Nr5a2 promoter also has a potential CREB-binding site. (nycu.edu.tw)
  • In addition, FSH and TGFβ1 increased CRTC2 binding to the Cyp19a1 PII-promoter and Nr5a2 promoter at 24 h, with CREB bound constitutively. (nycu.edu.tw)
  • As the figure shows, MC1R activates the cyclic AMP (cAMP) response-element binding protein (CREB). (cusabio.com)
Kinase11
  • Evidence is provided to support the possible involvement of extracellular signal-regulated kinase 1/2 (ERK1/2) and phosphatidylinositol-3 kinase/ protein kinase B (PI3K/AKT) pathways in EGCG-mediated protection against corticosterone-induced neuron injuries. (semanticscholar.org)
  • Salicylic acid and aspirin inhibit the activity of RSK2 kinase and repress RSK2-dependent transcription of cyclic AMP response element binding protein- and NF-kappa B-responsive genes. (drugbank.com)
  • Our findings indicate that ribosomal S6 kinase 2 (RSK2), a 90-kDa ribosomal S6 kinase with a critical role as an effector of the RAS-mitogen-activated protein kinase pathway and a regulator of immediate early gene transcription is a target for inhibition by the NSAIDs. (drugbank.com)
  • Intranasal delivery of insulin was associated with greater preservation of the phosphatidylinositol 3-kinase signaling pathway involving Akt, cyclic AMP response element binding protein,and glycogen synthase kinase 3beta but did not alter extracellular signal-regulated kinase, mitogen-activated protein kinase/extracellular signal-regulated kinase, or c-Jun amino-terminal kinase. (healthpartners.com)
  • In summary, extracellular signal-regulated kinase activation is required for many transcription factor responses to lysophosphatidic acid and epidermal growth factor, however it is not synergistic. (ersjournals.com)
  • One key proliferative signalling mediator is the extracellular signal-regulated kinase (ERK), a member of the mitogen-activation protein kinase (MAPK) family, and both LPA and EGF signal through ERK in many cell types 6 , 7 . (ersjournals.com)
  • Serum/glucticocorticoid kinase (SGK) phosphorylates SRF at serine residue 103 in response to multiple upstream stimuli (Tyan et al, 2008). (reactome.org)
  • Ligand binding to the four closely related members of this RTK family -epidermal growth factor receptor (EGFR, also known as ErbB-1 or HER1), ErbB-2 (HER2), ErbB-3 (HER3), and ErbB-4 (HER4)-induces the formation of receptor homo- and heterodimers and the activation of the intrinsic kinase domain, resulting in phosphorylation on specific tyrosine residues (pY) within the cytoplasmic tail. (uni-freiburg.de)
  • The objective of this study was to determine whether the increased AKT or MAPK kinase-1/2 (MEK1/2) activity observed in endometriotic stromal cells (OSIS) from ovarian endometriomas influences levels of PR protein. (webpediatrica.com)
  • Provided the limited precedent, collection of a suitable collection to screen a fresh class of focus on, such as for example an inositol phosphate kinase, is certainly a critical element of the complete HTS technique. (immune-source.com)
  • For the existing research we posited the fact that even more hydrophobic nucleotide-binding site of the inositol phosphate kinase would provide a possibly even more tractable focus on [23]. (immune-source.com)
Kinases7
  • HASM cells grown in vitro proliferate in response to a variety of stimuli, including G protein-coupled receptor (GPCR) agonists as well as peptide growth factors acting via receptor tyrosine kinases (RTKs) 1 , 2 . (ersjournals.com)
  • A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. (bvsalud.org)
  • The ErbB family of receptor tyrosine kinases (RTKs) couples binding of extracellular growth factor ligands to intracellular signaling pathways regulating diverse biologic responses, including proliferation, differentiation, cell motility, and survival. (uni-freiburg.de)
  • Activation of extracellular signal-regulated kinases, NF-kappa B, and cyclic adenosine 5'-monophosphate response element-binding protein in lung neutrophils occurs by differing mechanisms after hemorrhage or endotoxemia. (uchicago.edu)
  • Kinases can cause posttranslational modifications of the progesterone receptor (PR) to influence cellular localization and protein stability. (webpediatrica.com)
  • Our selection of a collection was influenced with the recognition the fact that substrate binding storage compartments of inositol phosphate kinases are extremely electropositive buy Protopanaxatriol [7,8,21,22]. (immune-source.com)
  • Using the nucleotide-binding sites of proteins TNR kinases specifically at heart as drug-targets, several chemical libraries have already been curated that consist of substances either knownor forecasted and purified to homogeneity [8]. (immune-source.com)
Phosphorylation6
  • Xu Y, Cao Z, Khan I, Luo Y. Gotu Kola (Centella Asiatica) extract enhances phosphorylation of cyclic AMP response element binding protein in neuroblastoma cells expressing amyloid beta peptide. (bestpricenutrition.com)
  • Recent studies have demonstrated that light induces the expression of Fos and phosphorylation of the cyclic-AMP response element-binding protein in the rodent suprachiasmatic nuclei, the location of a master circadian pacemaker in mammals, suggesting that these transcription factors may mediate the effects of light on the circadian clock. (elsevier.com)
  • The purpose of this study was to determine the effects of aging upon light-induced phase-shifting of circadian locomotor activity rhythms, Fos protein expression and cyclic-AMP response element-binding protein phosphorylation in the suprachiasmatic nuclei. (elsevier.com)
  • Aging was also associated with a deficit in cyclic-AMP response element-binding protein phosphorylation by light. (elsevier.com)
  • A selective increase in phosphorylation of cyclic AMP response element-binding protein in hippocampal CA1 region of male, but not female, rats following contextual fear and passive avoidance conditioning. (bvsalud.org)
  • Increased expression of MITF and its activation by phosphorylation (P) stimulate the transcription of tyrosinase (TYR) , tyrosinase-related protein 1 (TYRP1) , and dopachrome tautomerase (DCT) , which produce melanin. (cusabio.com)
Transcription factor5
  • The current study established that cAMP-responsive-element-binding protein H (CREBH), an acute-phase transcription factor, enhances very-low-density lipoprotein (VLDL) assembly and secretion by upregulating apolipoprotein B (apoB) expression and contributes to metabolic inflammation-associated hyperlipoproteinemia induced by TNFα, lipopolysaccharides (LPS), and high-fat diet (HFD) in mice. (qub.ac.uk)
  • This novel finding establishes CREBH as the first transcription factor that regulates apoB expression on the transcriptional level and the subsequent VLDL biosynthesis in response to metabolic inflammation. (qub.ac.uk)
  • Activation of the inhibitory guanine nucleotide-binding protein and of ERK signalling pathways were required for most transcription factor responses to LPA. (ersjournals.com)
  • We now demonstrate that the transcription factor AP-1 is modestly induced by cAMP in S49 cells and induced to significantly higher levels by cAMP in the presence of E1A proteins. (princeton.edu)
  • BACKGROUND AND PURPOSE Prostaglandin E 2 (PGE 2 ) stimulation of the G protein-coupled prostanoid EP 1 receptor was found to up-regulate the expression of Nur-related factor 1 (Nurr1) (NR4A2), a transcription factor in the NR4A subfamily of nuclear receptors. (arizona.edu)
Cell Proliferation1
  • The single-stranded DNA and RNA binding protein, Puralpha, has recently received special attention as this protein, by associating with the specific nucleotide sequence (GGN repeats) and/or several important cellular and viral proteins regulates crucial biological events such as transcription, replication, and cell proliferation. (nih.gov)
MRNA and protein expression4
  • Specifically, overexpression of CREBH significantly induced mRNA and protein expression of apoB in McA-7777 cells. (qub.ac.uk)
  • Activation of CREBH in inflammation enhances mRNA and protein expression of apoB. (qub.ac.uk)
  • We evaluated receptor mRNA and protein expression levels and effects of octreotide, pasireotide, and cabergoline on ACTH secretion by cultured human corticotroph adenoma cells. (webpediatrica.com)
  • Real-time PCR and Western blotting were used to assess mRNA and protein expression of cartonectin. (webpediatrica.com)
PCREB1
  • The number of cells in superficial and deep dorsal horn laminae at the L4-L5 level immunostained for phosphorylated cAMP response element binding protein (pCREB) were quantified. (asahq.org)
Genes3
  • Gene categorization showed that most of the differentially expressed genes are involved in oxidative stress response, and pathway analysis further revealed that Nrf2-mediated oxidative stress pathway is the top of the ITC-modulated signaling pathway. (researchgate.net)
  • Early prenatal exposure upregulated expression of genes associated with oxygen and nutrient transport, including hypoxia inducible factor 3α (HIF3α), peroxisome proliferator-activated receptor alpha (PPARα), and insulin-like growth binding factor 1 (IGFBP1), in the placenta of CTL diet males exposed to EPS. (biomedcentral.com)
  • E1A proteins and cAMP work in synergy to activate several of these genes. (princeton.edu)
Ischemia1
  • 2 Cerebral ischemia can trigger inflammatory responses, including the activation of microglia, macrophages, neutrophils, and dendritic cells. (researchsquare.com)
Neurons1
  • Additionally, luteolin treatment improved mitochondrial and locomotory activities in primary neurons and Caenorhabditis elegans expressing an expanded polyglutamine tract of the huntingtin protein. (biomedcentral.com)
Nucleotide2
  • This has involved the study of noradrenergic mechanisms in the actions of antidepressant drugs and of cyclic nucleotide phosphodiesterases as potential targets for novel antidepressant, anxiolytic, and memory-enhancing drugs. (buffalo.edu)
  • He is a member of a number of scientific and professional societies, including the American Society for Pharmacology and Experimental Therapeutics and the Society for Neuroscience, is a Fellow of the American College of Neuropsychopharmacology, and chaired the Gordon Research Conference on Cyclic Nucleotide Phosphodiesterases. (buffalo.edu)
Receptor1
  • Ovarian granulosa cells treated with FSH displayed increased aromatase protein at 24 h post-treatment, which subsided by 48 h, while TGFβ1 acting through its type 1 receptor augmented the action of FSH with a greater and longer effects. (nycu.edu.tw)
Acoustic startle response1
  • A double-blind, placebo-controlled study on the effects of Gotu Kola (Centella asiatica) on acoustic startle response in healthy subjects. (lykon.de)
Stimuli1
  • Aging is associated with a variety of alterations in circadian rhythms, including changes in the response to environmental stimuli. (elsevier.com)
CAMP2
  • This protein functions to integrate both calcium and cAMP signals. (bvsalud.org)
  • Cytoplasmic levels of c-fos and junB mRNAs are rapidly increased by cAMP, and the induction is substantially stronger in the presence of E1A protein. (princeton.edu)
Pathways1
  • In the sevoflurane anesthetized neonatal rat model, treatment with HPD reduced neuronal degeneration, hippocampal inflammation, and improvised memory, learning, and cognitive responses by modulating the PI3/Akt/PTEN and NF-κB signaling pathways. (semanticscholar.org)
Carbohydrates2
  • Basidiocarps of A. hygrometricus are rich in proteins, carbohydrates, minerals, crude fibre and essential amino acids with lower concentration of fat. (fulltxt.org)
  • 4 Edible mushrooms are low calorie-low fat food supplement with generous amount of proteins, carbohydrates, vitamins, minerals amino acids 5 and dietary fibre. (fulltxt.org)
Targets1
  • Finally, real-time polymerase chain reaction (PCR) and Western blotting confirmed the gene expression and protein products of the major targets by ITCs. (researchgate.net)
Mice1
  • While agrin was initially postulated to be the inductive molecule that initiates synaptogenesis, this model has been modified in response to work showing that postsynaptic differentiation can develop in the absence of innervation, and that synapses can form in transgenic mice in which the agrin gene is ablated. (mdpi.com)
Amino1
  • Basidiocarp of this macrofungi contains considerable amount of carbohydrate, protein, fibre, minerals, vitamins, essential amino acids and very minute concentration of fat. (fulltxt.org)
Transcriptional1
  • The regulatory protein of the human neurotropic virus, JCV, T-antigen, which interacts with Puralpha, decreased transcriptional activity of the Puralpha promoter. (nih.gov)
Cells6
  • HASM cells show increased mitogenesis in response to LPA, a GPCR mitogen, and to epidermal growth factor (EGF), an RTK growth factor. (ersjournals.com)
  • Interestingly, HASM cells treated with LPA together with EGF show a strikingly synergistic stimulation of mitogenesis, much greater than the sum of the responses to the two agents alone 4 , 5 . (ersjournals.com)
  • In histopathological examinations (toluidine blue (T.B.), major basic protein (MBP), CD4, IL-4, IL-5, eotaxin, TARC and IgE), the wasabi rhizome-containing HR-AD diet (5% and 10%) significantly reduced the number of positive stained cells. (researchgate.net)
  • Here, we found that coffee ingredients, namely caffeine and theobromine, decreased the protein level of CLDN2 in human lung adenocarcinoma-derived A549 cells. (bvsalud.org)
  • In a protein-stability assay using cycloheximide, CLDN2 protein levels decreased faster in caffeine-treated cells than in vehicle-treated cells. (bvsalud.org)
  • Inhibiting AKT with MK-2206 or MEK1/2 with U0126 for 24 hours in the absence of R5020 increased total and nuclear PRA and PRB protein levels in OSIS but not in eutopic endometrial stromal cells from disease-free patients from disease-free patients. (webpediatrica.com)
Mechanisms1
  • Mechanisms of lung neutrophil activation after hemorrhage or endotoxemia: roles of reactive oxygen intermediates, NF-kappa B, and cyclic AMP response element binding protein. (uchicago.edu)
Expression3
  • Co-expression of JCV T-antigen and Puralpha had no significant effect on the suppression of Puralpha gene transcription by either protein. (nih.gov)
  • There were no statistically significant differences in sst 5 and D 2 R mRNA expression or in sst 2 , sst 5 , and D 2 R protein expression between both groups of corticotroph adenomas. (webpediatrica.com)
  • Whether sustained normocortisolism induced by medical therapy induces re-expression of functional sst 2 protein in corticotroph adenomas and whether this increases the ACTH-lowering potency of octreotide remains to be established. (webpediatrica.com)
Molecular1
  • These data indicate that there are dramatic changes in light-activated molecular responses in the suprachiasmatic nuclei of old hamsters, and suggest that these molecular changes may underlie age-related changes in the effects of light on the circadian clock system. (elsevier.com)
Activation2
  • Rapid activation of Rho was observed in response to LPA but not to EGF. (ersjournals.com)
  • Activation of activator protein‐1 is synergistic, and Rho activation by lysophosphatidic acid is required for synergism in both activator protein‐1 activation and mitogenesis. (ersjournals.com)
Enhances1
  • The Crohn's disease polymorphism, ATG16L1 T300A, alters the gut microbiota and enhances the local Th1/Th17 response. (bu.edu)
Levels2
  • Young (three to four months) and old (18-22 months) male golden hamsters free-running in constant darkness were exposed to 5-min monochromatic light pulses of different irradiance levels, at circadian time 19, after which either steady-state phase shifts of locomotor activity rhythms were measured, or else immunocytochemistry for Fos or for phospho-cyclic-AMP response element-binding protein was performed. (elsevier.com)
  • Herein, we demonstrate that FSHCTGFβ1 increased LRH1 and SF1 protein levels, and their binding to the Cyp19a1 PII-promoter evidenced, determined by chromatin immunoprecipitation analysis. (nycu.edu.tw)
Sites2
  • To ameliorate this issue, interest is continuing to grow in rendering screening process more efficient, with the curation and program of smaller, concentrated libraries that focus on proteins households with functionally or chemically related binding sites [17]. (immune-source.com)
  • Such ligand-binding sites will be expected and then be successfully occupied by polar substances that usually do not easily combination cell buy Protopanaxatriol membranes, hence possibly deeming inositol phosphate binding storage compartments to become undruggable [23]. (immune-source.com)
Development1
  • Recent advances in protein structure determination and drug development have allowed for generation of highly specific PI3Kγ inhibitors, with the first now in clinical trials for several oncology indications. (nature.com)
Application of DNA-based tests for diagnosis of spinal muscular atrophy in Saudi Arabia
Application of DNA-based tests for diagnosis of spinal muscular atrophy in Saudi Arabia (extranet.who.int)
CREBH mediates metabolic inflammation to hepatic VLDL overproduction and hyperlipoproteinemia<...
CREBH mediates metabolic inflammation to hepatic VLDL overproduction and hyperlipoproteinemia<... (pure.qub.ac.uk)
Cells | Free Full-Text | Nerve, Muscle, and Synaptogenesis
Cells | Free Full-Text | Nerve, Muscle, and Synaptogenesis (mdpi.com)
PDF] Protective effects of a green tea polyphenol, epigallocatechin-3-gallate, against sevoflurane-induced neuronal apoptosis...
PDF] Protective effects of a green tea polyphenol, epigallocatechin-3-gallate, against sevoflurane-induced neuronal apoptosis... (semanticscholar.org)
The role of PI3Kγ in the immune system: new insights and translational implications | Nature Reviews Immunology
The role of PI3Kγ in the immune system: new insights and translational implications | Nature Reviews Immunology (nature.com)
Salicylic acid and aspirin inhibit the activity of RSK2 kinase and repress RSK2-dependent transcription of cyclic AMP response...
Salicylic acid and aspirin inhibit the activity of RSK2 kinase and repress RSK2-dependent transcription of cyclic AMP response... (go.drugbank.com)
Publications | National Institute on Alcohol Abuse and Alcoholism | Update on the Genetics of Alcoholism
Publications | National Institute on Alcohol Abuse and Alcoholism | Update on the Genetics of Alcoholism (pubs.niaaa.nih.gov)
Plus it
Plus it (jneurosci.org)
Plus it
Plus it (jneurosci.org)
ZFIN Publication: Dworkin et al., 2007
ZFIN Publication: Dworkin et al., 2007 (zfin.org)
Antineuroinflammatory effects of dl-3-n-butylphthalide conferred by stimulation of Foxp3 and Ki-67 in an ischemic stroke model ...
Antineuroinflammatory effects of dl-3-n-butylphthalide conferred by stimulation of Foxp3 and Ki-67 in an ischemic stroke model ... (researchsquare.com)
Mary Stevenson | Harvard Catalyst Profiles | Harvard Catalyst
Mary Stevenson | Harvard Catalyst Profiles | Harvard Catalyst (connects.catalyst.harvard.edu)
Tony Wyss-Coray, PhD's Profile | Stanford Profiles
Tony Wyss-Coray, PhD's Profile | Stanford Profiles (profiles.stanford.edu)
Neuronal cell-based high-throughput screen for enhancers of mitochondrial function reveals luteolin as a modulator of...
Neuronal cell-based high-throughput screen for enhancers of mitochondrial function reveals luteolin as a modulator of... (bmcbiol.biomedcentral.com)
Clonidine-induced Neuronal Activation in the Spinal Cord Is Altered after Peripheral Nerve Injury | Anesthesiology | American...
Clonidine-induced Neuronal Activation in the Spinal Cord Is Altered after Peripheral Nerve Injury | Anesthesiology | American... (pubs.asahq.org)
Plus it
Plus it (erj.ersjournals.com)
(researchgate.net)
Find Research outputs - Augusta University Research Profiles
Find Research outputs - Augusta University Research Profiles (augusta.pure.elsevier.com)
Bioassay - Usa-China BIO
Bioassay - Usa-China BIO (usachinabio.com)
Systemic Inhibition of CREB is Well-tolerated in vivo<...
Systemic Inhibition of CREB is Well-tolerated in vivo<... (ohsu.pure.elsevier.com)
Acupuncture stimulation improves scopolamine-induced cognitive impairment via activation of cholinergic system and regulation...
Acupuncture stimulation improves scopolamine-induced cognitive impairment via activation of cholinergic system and regulation... (bmccomplementmedtherapies.biomedcentral.com)
Maternal DHA supplementation influences sex-specific disruption of placental gene expression following early prenatal stress |...
Maternal DHA supplementation influences sex-specific disruption of placental gene expression following early prenatal stress |... (bsd.biomedcentral.com)
Korea University(SEOUL) - Research output - Korea University
Korea University(SEOUL) - Research output - Korea University (koreauniv.pure.elsevier.com)
R. Suzanne Zukin - Publications - Albert Einstein College of Medicine
R. Suzanne Zukin - Publications - Albert Einstein College of Medicine (einstein.pure.elsevier.com)