Cyclic AMP Receptor Protein: A transcriptional regulator in prokaryotes which, when activated by binding cyclic AMP, acts at several promoters. Cyclic AMP receptor protein was originally identified as a catabolite gene activator protein. It was subsequently shown to regulate several functions unrelated to catabolism, and to be both a negative and a positive regulator of transcription. Cell surface cyclic AMP receptors are not included (CYCLIC AMP RECEPTORS), nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins, which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES.Receptors, Cyclic AMP: Cell surface proteins that bind cyclic AMP with high affinity and trigger intracellular changes which influence the behavior of cells. The best characterized cyclic AMP receptors are those of the slime mold Dictyostelium discoideum. The transcription regulator CYCLIC AMP RECEPTOR PROTEIN of prokaryotes is not included nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Bacterial Proteins: Proteins found in any species of bacterium.DNA-Directed RNA Polymerases: Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Lac Operon: The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.Galactose: An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Pectobacterium chrysanthemi: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria that causes vascular wilts on a wide range of plant species. It was formerly named Erwinia chrysanthemi.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Genes, Bacterial: The functional hereditary units of BACTERIA.DNA Footprinting: A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Regulon: In eukaryotes, a genetic unit consisting of a noncontiguous group of genes under the control of a single regulator gene. In bacteria, regulons are global regulatory systems involved in the interplay of pleiotropic regulatory domains and consist of several OPERONS.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Lactose: A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.Sigma Factor: A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.Mutagenesis, Insertional: Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Genes, Regulator: Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.Electrophoretic Mobility Shift Assay: An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.Allosteric Regulation: The modification of the reactivity of ENZYMES by the binding of effectors to sites (ALLOSTERIC SITES) on the enzymes other than the substrate BINDING SITES.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.AraC Transcription Factor: A transcription factor found in BACTERIA that positively and negatively regulates the expression of proteins required for the uptake and catabolism of L-ARABINOSE.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.ArabinoseAnaerobiosis: The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Dictyostelium: A genus of protozoa, formerly also considered a fungus. Its natural habitat is decaying forest leaves, where it feeds on bacteria. D. discoideum is the best-known species and is widely used in biomedical research.Adenylate Cyclase: An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Kinetics: The rate dynamics in chemical or physical systems.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Transcriptional Activation: Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Salmonella typhimurium: A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.Bucladesine: A cyclic nucleotide derivative that mimics the action of endogenous CYCLIC AMP and is capable of permeating the cell membrane. It has vasodilator properties and is used as a cardiac stimulant. (From Merck Index, 11th ed)Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.

Catabolic repression of secB expression is positively controlled by cyclic AMP (cAMP) receptor protein-cAMP complexes at the transcriptional level. (1/488)

SecB, a protein export-specific chaperone, enhances the export of a subset of proteins across cytoplasmic membranes of Escherichia coli. Previous studies showed that the synthesis of SecB is repressed by the presence of glucose in the medium. The derepression of SecB requires the products of both the cya and crp genes, indicating that secB expression is under the control of catabolic repression. In this study, two secB-specific promoters were identified. In addition, 5' transcription initiation sites from these two promoters were determined by means of secB-lacZ fusions and primer extension. The distal P1 promoter appeared to be independent of carbon sources, whereas the proximal P2 promoter was shown to be subject to control by the cyclic AMP (cAMP) receptor protein (CRP)-cAMP complexes. Gel-mobility shift studies showed that this regulation results from direct interaction between the secB P2 promoter region and the CRP-cAMP complex. Moreover, the CRP binding site on the secB gene was determined by DNase I footprinting and further substantiated by mutational analysis. The identified secB CRP binding region is centered at the -61.5 region of the secB gene and differed from the putative binding sites predicted by computer analysis.  (+info)

The cyclic AMP receptor protein is dependent on GcvA for regulation of the gcv operon. (2/488)

The Escherichia coli gcv operon is transcriptionally regulated by the GcvA, GcvR, Lrp, and PurR proteins. In this study, the cyclic AMP (cAMP) receptor protein (CRP) is shown to be involved in positive regulation of the gcv operon. A crp deletion reduced expression of a gcvT-lacZ fusion almost fourfold in glucose minimal (GM) medium. The phenotype was complemented by both the wild-type crp gene and four crp alleles that encode proteins with amino acid substitutions in known activating regions of CRP. A cyaA deletion also resulted in a fourfold decrease in gcvT-lacZ expression, and wild-type expression was restored by the addition of cAMP to the growth medium. A cyaA crp double deletion resulted in levels of gcvT-lacZ expression identical to those observed with either single mutation, showing that CRP and cAMP regulate through the same mechanism. Growth in GM medium plus cAMP or glycerol minimal medium did not result in a significant increase in gcvT-lacZ expression. Thus, the level of cAMP present in GM medium appears to be sufficient for regulation by CRP. DNase I footprint analysis showed that CRP binds and protects two sites centered at bp -313 (site 1) and bp -140 (site 2) relative to the transcription initiation site, but a mutational analysis demonstrated that only site 1 is required for CRP-mediated regulation of gcvT-lacZ expression. Expression of the gcvT-lacZ fusion in a crp gcvA double mutant suggested that CRP's role is dependent on the GcvA protein.  (+info)

Role of ArgR in activation of the ast operon, encoding enzymes of the arginine succinyltransferase pathway in Salmonella typhimurium. (3/488)

The ast operon, encoding enzymes of the arginine succinyltransferase (AST) pathway, was cloned from Salmonella typhimurium, and the nucleotide sequence for the upstream flanking region was determined. The control region contains several regulatory consensus sequences, including binding sites for NtrC, cyclic AMP receptor protein (CRP), and ArgR. The results of DNase I footprintings and gel retardation experiments confirm binding of these regulatory proteins to the identified sites. Exogenous arginine induced AST under nitrogen-limiting conditions, and this induction was abolished in an argR derivative. AST was also induced under carbon starvation conditions; this induction required functional CRP as well as functional ArgR. The combined data are consistent with the hypothesis that binding of one or more ArgR molecules to a region between the upstream binding sites for NtrC and CRP and two putative promoters plays a pivotal role in modulating expression of the ast operon in response to nitrogen or carbon limitation.  (+info)

Mapping cyclic nucleotide-induced conformational changes in cyclicAMP receptor protein by a protein footprinting technique using different chemical proteases. (4/488)

CyclicAMP receptor protein (CRP) regulates transcription of numerous genes in Escherichia coli. Both cAMP and cGMP bind CRP, but only cAMP induces conformational changes that dramatically increase the specific DNA binding activity of the protein. We have shown previously that our protein footprinting technique is sensitive enough to detect conformational changes in CRP by cAMP [Baichoo N, Heyduk T. 1997. Biochemistry 36:10830-10836]. In this work, conformational changes in CRP induced by cAMP and cGMP binding were mapped and quantitatively analyzed by protein footprinting using iron complexed to diethylenetriaminepentaacetic acid ([Fe-DTPA]2-), iron complexed to ethylenediaminediacetic acid ([Fe-EDDA]), iron complexed to desferrioxamine mesylate ([Fe-HDFO]+), and copper complexed to o-phenanthroline ([(OP)2Cu]+) as proteases. These chemical proteases differ in size, charge, and hydrophobicity. Binding of cAMP to CRP resulted in changes in susceptibility to cleavage by all four proteases. Cleavage by [Fe-EDDA] and [Fe-DTPA]2- of CRP-cAMP detected hypersensitivities in the DNA-binding F alpha-helix, the interdomain hinge, and the ends of the C alpha-helix, which is involved in intersubunit interactions. [Fe-EDDA] and [Fe-DTPA]2- also detected reductions in cleavage in the D and E alpha-helices, which are involved in DNA recognition. Cleavage by [Fe-HDFO]+ of CRP-cAMP detected hypersensitivities in beta-strand 8, the B alpha-helix, as well as in parts of the F and C alpha-helices. [Fe-HDFO]+ also detected protections from cleavage in beta-strands 4 to 5 and their intervening loop, beta-strand 7, which is part of the nucleotide binding pocket, as well as in the D and E alpha-helices. Cleavage by [(OP)2Cu]+ of CRP-cAMP detected hypersensitivities in beta-strands 9 and 11 as well as in the D and E alpha-helices. [(OP)2Cu]+ also detected protections in the C alpha-helix , the interdomain hinge, and beta-strands 2-7. Binding of cGMP to CRP resulted in changes in susceptibility to cleavage only by [(OP)2Cu]+, which detected minor protections in beta-strands 3-7, the interdomain hinge, and the C alpha-helix. These results show that binding of cAMP causes structural changes in CRP in the nucleotide binding domain, the interdomain hinge, the DNA binding domain, and regions involved in intersubunit interaction. Structural changes induced by binding of cGMP appear to be very minor and confined to the nucleotide binding domain, the interdomain hinge, and regions involved in intersubunit interaction. Use of different cleaving agents in protein footprinting seems to give a more detailed picture of structural changes than the use of a single protease alone.  (+info)

The medium-/long-chain fatty acyl-CoA dehydrogenase (fadF) gene of Salmonella typhimurium is a phase 1 starvation-stress response (SSR) locus. (5/488)

Salmonella enterica serovar Typhimurium (S. typhimurium) is an enteric pathogen that causes significant morbidity in humans and other mammals. During their life cycle, salmonellae must survive frequent exposures to a variety of environmental stresses, e.g. carbon-source (C) starvation. The starvation-stress response (SSR) of S. typhimurium encompasses the genetic and physiological realignments that occur when an essential nutrient becomes limiting for bacterial growth. The function of the SSR is to produce a cell capable of surviving long-term starvation. This paper reports that three C-starvation-inducible lac fusions from an S. typhimurium C-starvation-inducible lac fusion library are all within a gene identified as fadF, which encodes an acyl-CoA dehydrogenase (ACDH) specific for medium-/long-chain fatty acids. This identification is supported by several findings: (a) significant homology at the amino acid sequence level with the ACDH enzymes from other bacteria and eukaryotes, (b) undetectable beta-oxidation levels in fadF insertion mutants, (c) inability of fad insertion mutants to grow on oleate or decanoate as a sole C-source, and (d) inducibility of fadF::lac fusions by the long-chain fatty acid oleate. In addition, the results indicate that the C-starvation-induction of fadF is under negative control by the FadR global regulator and positive control by the cAMP:cAMP receptor protein complex and ppGpp. It is also shown that the fadF locus is important for C-starvation-survival in S. typhimurium. Furthermore, the results demonstrate that fadF is induced within cultured Madin-Darby canine kidney (MDCK) epithelial cells, suggesting that signals for its induction (C-starvation and/or long-chain fatty acids) may be present in the intracellular environment encountered by S. typhimurium. However, fadF insertion mutations did not have an overt effect on mouse virulence.  (+info)

The molecular basis of the instability of a crp- mutation in Escherichia coli. (6/488)

We have described a rapid spontaneous conversion in the stationary phase of Escherichia coli strain DOO (crp-) cells as a whole population to crp+ state (Sugino and Morita, 1994). In this paper we have tried to elucidate the molecular basis of this unidirectional conversion by cloning and sequencing of the crp gene in their crp+ and crp- states. We have found that in the original crp- strain, an IS2 element has been inserted between its original promoter and the coding region of the crp gene in the so-called orientation II (Ahmed et al., 1981), accompanied by an 11 bp deletion. Unexpectedly, the crp+ "revertants" derived from the crp- mutant had no difference in sequence from the crp-, either in the coding or the regulatory region. This suggests that a change at another locus, such that this change somehow activates the expression of the crp gene to the level of a normal crp+, is responsible for the apparent reversion from crp- to crp+.  (+info)

Role of multiple CytR binding sites on cooperativity, competition, and induction at the Escherichia coli udp promoter. (7/488)

The CytR repressor fulfills dual roles as both a repressor of transcription from promoters of the Escherichia coli CytR regulon and a co-activator in some circumstances. Transcription is repressed by a three-protein complex (cAMP receptor protein (CRP)-CytR-CRP) that is stabilized by cooperative interactions between CRP and CytR. However, cooperativity also means that CytR can recruit CRP and, by doing so, can act as a co-activator. The central role of cooperativity in regulation is highlighted by the fact that binding of the inducer, cytidine, to CytR is coupled to CytR-CRP cooperativity; this underlies the mechanism for induction. Similar interactions at the different promoters of the CytR regulon coordinate expression of the transport proteins and enzymes required for nucleoside catabolism but also provide differential expression of these genes. A fundamental question in both prokaryotic and eukaryotic gene regulation is how combinatorial mechanisms of this sort regulate differential expression. Recently, we showed that CytR binds specifically to multiple sites in the E. coli deoP promoter, thereby providing competition for CRP binding to CRP operator site 1 (CRP1) and CRP2 as well as cooperativity. The effect of the competition at this promoter is to negate the role of CytR in recruiting CRP. Here, we have used quantitative footprint and mobility shift analysis to investigate CRP and CytR binding to the E. coli udp promoter. Here too, we find that CytR both cooperates and competes for CRP binding. However, consistent with both the distribution of CytR recognition motifs in the sequence of the promoter and the regulation of the promoter, the competition is limited to CRP2. When cytidine binds to CytR, the effect on cooperativity is very different at the udp promoter than at the deoP2 promoter. Cooperativity with CRP at CRP1 is nearly eliminated, but the effect on CytR-CRP2 cooperativity is negligible. These results are discussed in relation to the current structural model of CytR in which the core, inducer-binding domain is tethered to the helix-turn-helix, DNA-binding domain via flexible peptide linkers.  (+info)

Control of acid resistance in Escherichia coli. (8/488)

Acid resistance (AR) in Escherichia coli is defined as the ability to withstand an acid challenge of pH 2.5 or less and is a trait generally restricted to stationary-phase cells. Earlier reports described three AR systems in E. coli. In the present study, the genetics and control of these three systems have been more clearly defined. Expression of the first AR system (designated the oxidative or glucose-repressed AR system) was previously shown to require the alternative sigma factor RpoS. Consistent with glucose repression, this system also proved to be dependent in many situations on the cyclic AMP receptor protein. The second AR system required the addition of arginine during pH 2.5 acid challenge, the structural gene for arginine decarboxylase (adiA), and the regulator cysB, confirming earlier reports. The third AR system required glutamate for protection at pH 2.5, one of two genes encoding glutamate decarboxylase (gadA or gadB), and the gene encoding the putative glutamate:gamma-aminobutyric acid antiporter (gadC). Only one of the two glutamate decarboxylases was needed for protection at pH 2.5. However, survival at pH 2 required both glutamate decarboxylase isozymes. Stationary phase and acid pH regulation of the gad genes proved separable. Stationary-phase induction of gadA and gadB required the alternative sigma factor sigmaS encoded by rpoS. However, acid induction of these enzymes, which was demonstrated to occur in exponential- and stationary-phase cells, proved to be sigmaS independent. Neither gad gene required the presence of volatile fatty acids for induction. The data also indicate that AR via the amino acid decarboxylase systems requires more than an inducible decarboxylase and antiporter. Another surprising finding was that the sigmaS-dependent oxidative system, originally thought to be acid induced, actually proved to be induced following entry into stationary phase regardless of the pH. However, an inhibitor produced at pH 8 somehow interferes with the activity of this system, giving the illusion of acid induction. The results also revealed that the AR system affording the most effective protection at pH 2 in complex medium (either Luria-Bertani broth or brain heart infusion broth plus 0.4% glucose) is the glutamate-dependent GAD system. Thus, E. coli possesses three overlapping acid survival systems whose various levels of control and differing requirements for activity ensure that at least one system will be available to protect the stationary-phase cell under naturally occurring acidic environments.  (+info)

*Gua Operon

"Downregulation of the Escherichia coli guaB promoter by upstream-bound cyclic AMP receptor protein". Journal of Bacteriology. ... The gua operon is repressed by GMP and is induced by AMP. Similarly AMP synthesis is repressed by AMP itself while it is ... While growing on media in which growth rates are low, cAMP binds to a cAMP Receptor Protein(CRP) forming a CRP-cAMP complex ... One method through which this happens is the DnaA protein. DnaA is a protein which recognises the origin of replication, ...

*RyeE RNA

It has also been shown that cyaR expression is tightly controlled by the cyclic AMP receptor protein, CRP. A comparative ... This small RNA was shown to be bound by the Hfq protein. This RNA has been renamed as CyaR for (cyclic AMP-activated RNA). It ...

*Spot 42 RNA

Polayes DA, Rice PW, Garner MM, Dahlberg JE (July 1988). "Cyclic AMP-cyclic AMP receptor protein as a repressor of ... cAMP-receptor protein) complex. Spot42 is found in 100 - 200 copies per cell when cells are grown in glucose, and is reduced 3- ... Furthermore, in a spf deletion mutant a gene encoding a pirin-like protein was upregulated 16 folds. Pirin has key roles in the ... In E. coli Spot 42 accumulates under growth in the presence of glucose (i.e., when adenosine 3′,5′-cyclic monophosphate (cAMP) ...

*PBAD promoter

araC encodes the AraC protein, which regulates activity of both the PBAD and PC promoters. The cyclic AMP receptor protein CAP ... Schleif R. AraC protein, regulation of the L-arabinose operon in Escherichia coli, and the light switch mechanism of AraC ... The N-terminal arm of AraC interacts with its DNA binding domain, allowing two AraC proteins to bind to the O2 and I1 operator ... Schleif R. AraC protein: a love-hate relationship. Bioessays 2003, 25:274-282. Reed WL, Schleif RF. Hemiplegic Mutations in ...

*Index of biochemistry articles

... cyclic AMP receptor - cyclic AMP receptor protein - cyclic AMP-responsive DNA-binding protein - cyclic electron flow - Cyclic ... prostaglandin receptor - protein - protein biosynthesis - Protein Data Bank - protein design - protein expression - protein ... fusion oncogene protein - G protein - G protein-coupled receptor - G3P - GABA - GABA receptor - GABA-A receptor - gag-onc ... protein P16 - protein P34cdc2 - protein precursor - protein structure prediction - protein subunit - protein synthesis - ...

*Ira Pastan

... showing that cyclic AMP and its receptor protein CRP (cyclic AMP receptor protein) positively regulated the activity of many ... These studies serve as a paradigm for the mechanism of action of cyclic AMP and steroid hormones on gene expression in animal ... Pastan, with his colleague Jesse Roth, was the first to clearly demonstrate the presence of specific protein receptors on the ... Besides being one of the first 3 laboratories to obtain the DNA sequence of the EGF receptor, they showed that the EGF receptor ...

*RpoS

Flanking rpoSp are two putative cAMP-CRP (cyclic AMP-cAMP receptor protein) binding sites that seem to control rpoS ... Sigma factors are proteins that regulate transcription in bacteria. Sigma factors can be activated in response to different ... However, the RNA-binding protein Hfq is implicated in the process. Hfq binds to rpoS mRNA in vitro and may thereby modify rpoS ... The gene rpoS (RNA polymerase, sigma S) encodes the sigma factor sigma-38 (σ38, or RpoS), a 37.8 kD protein in Escherichia coli ...

*Autoinducer-2

"Cyclic AMP (cAMP) and cAMP Receptor Protein Influence both Synthesis and Uptake of Extracellular Autoinducer 2 in Escherichia ... Rezzonico, F.; Duffy, B. (2008). "Lack of genomic evidence of AI-2 receptors suggests a non-quorum sensing role for luxS in ... Although the luxS gene, which encodes the protein responsible for AI-2 production is widespread, the latter has mainly a ... An unequivocally AI-2 related behavior was found to be restricted primarily to organisms bearing known AI-2 receptor genes. ...

*CAMP receptor protein

In Escherichia coli, cyclic AMP receptor protein (CRP) can regulate the transcription of more than 100 genes. The signal to ... cAMP receptor protein (CRP; also known as catabolite activator protein, CAP) is a regulatory protein in bacteria. CRP protein ... CRP then activates transcription through direct protein-protein interactions with RNA polymerase. The genes regulated by CRP ... in which protein-protein interactions between CRP and RNA polymerase assist binding of RNA polymerase to the promoter. Busby S ...

*Sucrose phosphorylase

First discovered in Gram-negative bacteria, both Cyclic AMP (cAMP) and cAMP Receptor Protein (CRP) function in sucrose ... The covalent complex was experimentally isolated by chemical modification of the protein using NaIO4 after addition of the ...

*Cyclic AMP receptors

... from slime molds are a distinct family of G-protein coupled receptors. These receptors control development ... In D. discoideum, the cyclic AMP receptors coordinate aggregation of individual cells into a multicellular organism, and ... cAMP receptor protein Devreotes PN, Kimmel AR, Johnson RL, Klein PS, Sun TJ, Saxe III CL (1988). "A chemoattractant receptor ... in a manner reminiscent of the rhodopsins and other receptors believed to interact with G-proteins. However, while a similar 3D ...

*Human iron metabolism

"Hepcidin directly inhibits transferrin receptor 1 expression in astrocytes via a cyclic AMP-protein kinase A pathway". Glia. 59 ... To be absorbed, dietary iron can be absorbed as part of a protein such as heme protein or iron must be in its ferrous Fe2+ form ... Most cell types take up iron primarily through receptor-mediated endocytosis via transferrin receptor 1 (TFR1), transferrin ... the interactions of transferrin receptor and transferrin receptor 2 with transferrin and the hereditary hemochromatosis protein ...

*GPR6

"Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite ... G protein-coupled receptor 6, also known as GPR6, is a protein which in humans is encoded by the GPR6 gene. GPR6 is a member of ... GPR6 up-regulates cyclic AMP levels and promotes neurite outgrowth. Lysophospholipid receptor GRCh38: Ensembl release 89: ... 1995). "Isolation of three novel human genes encoding G protein-coupled receptors". DNA Cell Biol. 14 (1): 25-35. doi:10.1089/ ...

*Muscarinic acetylcholine receptor M5

... stimulation of this receptor is known to effectively decrease cyclic AMP levels and downregulate the activity of protein kinase ... 1996). "Phosphorylation of human m1 muscarinic acetylcholine receptors by G protein-coupled receptor kinase 2 and protein ... is a member of the G protein-coupled receptor superfamily of integral membrane proteins. It is coupled to Gq protein. Binding ... "Signaling through transforming G protein-coupled receptors in NIH 3T3 cells involves c-Raf activation. Evidence for a protein ...

*Dopamine receptor D1

This G-protein coupled receptor is Gs/a coupled and indirectly activates cyclic AMP-dependent protein kinase, stimulating the ... Dopamine receptor D1, also known as DRD1, is a protein that in humans is encoded by the DRD1 gene. Based upon Northern blot and ... and various serotonin receptors Many typical and atypical antipsychotics are D1 receptor antagonists in addition to D2 receptor ... the D5 receptor, but the benzazepines generally are more selective for the D1 and D5 receptors versus the D2-like family. Some ...

*Glycogenesis

Epinephrine binds to a receptor protein that activates adenylate cyclase. The latter enzyme causes the formation of cyclic AMP ... G-Protein coupled receptor). When insulin binds to its receptor (insulin receptor), it results in the activation ( ... two molecules of cyclic AMP bind to the regulatory subunit of protein kinase A, which activates it allowing the catalytic ... Calcium ions or cyclic AMP (cAMP) act as secondary messengers. This is an example of negative control. The calcium ions ...

*Olfactory receptor

The G protein (Golf and/or Gs) in turn activates the lyase - adenylate cyclase - which converts ATP into cyclic AMP (cAMP). The ... Olfactory Receptor Database Human Olfactory Receptor Data Exploratorium (HORDE) Olfactory Receptor Protein at the US National ... These receptors are members of the class A rhodopsin-like family of G protein-coupled receptors (GPCRs). The olfactory ... Olfactory receptors (ORs), also known as odorant receptors, are expressed in the cell membranes of olfactory receptor neurons ...

*Sotalol

... the G-protein complex associated with the receptor cannot activate production of cyclic AMP, which is responsible for turning ... Sotalol non-selectively binds to both β1- and β2-adrenergic receptors preventing activation of the receptors by their ... Beta receptor blockade potentiates the antiarryhthmic actions of d-sotalol on re-entrant ventricular tachycardia in a canine ... Sotalol is a non-selective competitive beta-adrenergic receptor blocker that also exhibits Class III antiarrhythmic properties ...

*Diabetes insipidus

When released, ADH binds to V2 G-protein coupled receptors within the distal convoluted tubules, increasing cyclic AMP, which ... it acts on proteins called aquaporins and more specifically aquaporin 2 in the following cascade. ... couples with protein kinase A, stimulating translocation of the aquaporin 2 channel stored in the cytoplasm of the distal ...

*Dopamine receptor D4

... thereby reducing the intracellular concentration of the second messenger cyclic AMP. The human protein is coded by the DRD4 on ... The dopamine receptor D4 is a G protein-coupled receptor encoded by the DRD4 gene on chromosome 11 at 11p15.5. The structure of ... "Modulation of intracellular cyclic AMP levels by different human dopamine D4 receptor variants". Journal of Neurochemistry. 65 ... The D4 receptor is considered to be D2-like in which the activated receptor inhibits the enzyme adenylate cyclase, ...

*Olfactory receptor neuron

The activated OR in turn activates the intracellular G-protein, GOLF (GNAL), adenylate cyclase and production of cyclic AMP ( ... The surface of these cilia is covered with olfactory receptors, a type of G protein-coupled receptor. Each olfactory receptor ... but many separate olfactory receptor cells express ORs which bind the same set of odors. The axons of olfactory receptor cells ... An olfactory receptor neuron (ORN), also called an olfactory sensory neuron (OSN), is a transduction cell within the olfactory ...

*List of MeSH codes (D12.644)

... cyclic amp-dependent protein kinases MeSH D12.644.360.200.125.500 --- beta-adrenergic receptor kinase MeSH D12.644.360.200.150 ... smad6 protein MeSH D12.644.360.024.334.200.700 --- smad7 protein MeSH D12.644.360.024.334.500 --- smad proteins, receptor- ... tumor necrosis factor receptor-associated peptides and proteins MeSH D12.644.360.024.500.500 --- tnf receptor-associated factor ... rap gtp-binding proteins MeSH D12.644.360.525.475.100 --- rap1 gtp-binding proteins MeSH D12.644.360.525.500 --- ras proteins ...

*HRASLS3

This mechanism was postulated on the basis that the predominant signaling protein and receptor present in WAT are PGE2 and EP3 ... Inhibition of adenylyl cyclase decreases the conversion of cyclic AMP (cAMP) from ATP. Lower levels of cAMP decrease the ... PGE2 enters the signaling pathway binding to G protein-coupled receptor (EP3) which inhibits adenylyl cyclase. ... "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): ...

*G protein

Gαs activates the cAMP-dependent pathway by stimulating the production of cyclic AMP (cAMP) from ATP. This is accomplished by ... Whereas G proteins are activated by G protein-coupled receptors, they are inactivated by RGS proteins (for "Regulator of G ... Heterotrimeric G proteins, sometimes referred to as the "large" G proteins, are activated by G protein-coupled receptors and ... These include receptor antagonists, neurotransmitters, neurotransmitter reuptake, G protein-coupled receptors, G proteins, ...

*ADCY9

Patrizio M, Colucci M, Levi G (2001). "Human immunodeficiency virus type 1 Tat protein decreases cyclic AMP synthesis in rat ... It is regulated by a family of G protein-coupled receptors, protein kinases, and calcium. The type 9 adenylyl cyclase is a ... cyclic AMP generation, Ig production, and B-cell receptor expression". J. Allergy Clin. Immunol. 105 (5): 975-82. doi:10.1067/ ... Adenylyl cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. ...

*NFATC4

Chow CW, Davis RJ (Jan 2000). "Integration of calcium and cyclic AMP signaling pathways by 14-3-3". Molecular and Cellular ... NFATC4 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ... a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible ... Nuclear factor of activated T-cells, cytoplasmic 4 is a protein that in humans is encoded by the NFATC4 gene. The product of ...
Transcription activation at two semi-synthetic Escherichia coli promoters, CC(-41.5) and CC(-72.5), is dependent on the cyclic AMP receptor protein (CRP) that binds to sites centred 41.5 and 72.5 bp upstream from the respective transcription startpoints. An UP-element that can bind the C-terminal domain of the RNA polymerase (RNAP) alpha-subunit was cloned upstream of the DNA site for CRP at CC(-41.5) and downstream of the DNA site for CRP at CC(-72.5). In both cases CRP-dependent promoter activity was increased by the UP-element, but CRP-independent activity was not increased. DNase I footprinting was exploited to investigate the juxtaposition of bound CRP and RNAP alpha-subunits. In both cases, CRP and RNAP alpha-subunits occupy their cognate binding sites in ternary CRP-RNAP promoter complexes. RNAP alpha-subunits can occupy the UP-element in the absence of CRP, but this is not sufficient for open complex formation. The positive effects of binding RNAP alpha-subunits upstream of the DNA site ...
In bacteria, the level of cAMP varies depending on the medium used for growth. In particular, cAMP is low when glucose is the carbon source. This occurs through inhibition of the cAMP-producing enzyme, adenylyl cyclase, as a side-effect of glucose transport into the cell. The transcription factor cAMP receptor protein (CRP) also called CAP (catabolite gene activator protein) forms a complex with cAMP and thereby is activated to bind to DNA. CRP-cAMP increases expression of a large number of genes, including some encoding enzymes that can supply energy independent of glucose. cAMP, for example, is involved in the positive regulation of the lac operon. In an environment of a low glucose concentration, cAMP accumulates and binds to the allosteric site on CRP (cAMP receptor protein), a transcription activator protein. The protein assumes its active shape and binds to a specific site upstream of the lac promoter, making it easier for RNA polymerase to bind to the adjacent promoter to start ...
In bacteria, the level of cAMP varies depending on the medium used for growth. In particular, cAMP is low when glucose is the carbon source. This occurs through inhibition of the cAMP-producing enzyme, adenylyl cyclase, as a side-effect of glucose transport into the cell. The transcription factor cAMP receptor protein (CRP) also called CAP (catabolite gene activator protein) forms a complex with cAMP and thereby is activated to bind to DNA. CRP-cAMP increases expression of a large number of genes, including some encoding enzymes that can supply energy independent of glucose. cAMP, for example, is involved in the positive regulation of the lac operon. In an environment of a low glucose concentration, cAMP accumulates and binds to the allosteric site on CRP (cAMP receptor protein), a transcription activator protein. The protein assumes its active shape and binds to a specific site upstream of the lac promoter, making it easier for RNA polymerase to bind to the adjacent promoter to start ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
SoxS is the direct transcriptional activator of the member genes of the Escherichia coli superoxide regulon. At class I SoxS-dependent promoters, e.g. zwf and fpr, whose SoxS binding sites (soxbox) lie upstream of the -35 region of the promoter, activation requires the C-terminal domain of the RNA polymerase alpha-subunit, while at class II SoxS-dependent promoters, e.g. fumC and micF, whose binding sites overlap the -35 region, activation is independent of the alpha-CTD. To determine whether SoxS activation of its class I promoters shows the same helical phase-dependent spacing requirement as class I promoters activated by catabolite gene activator protein, we increased the 7 bp distance between the 20 bp zwf soxbox and the zwf -35 promoter hexamer by 5 bp and 11 bp, and we decreased the 15 bp distance between the 20 bp fpr soxbox and the fpr -35 promoter hexamer by the same amounts. In both cases, displacement of the binding site by a half or full turn of the DNA helix prevented ...
SWISS-MODEL Template Library (SMTL) entry for 1o3t.1. PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES
Assignment #3: Favorite Chime Structure: The cAMP Receptor Protein Assignment #4: Review Paper: Analysis of the eye developmental pathway in Drosophila using DNA microarrays Send Questions, Comments & Suggestions To Maureen. ...
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Aiba H.. The regulatory region of the Escherichia coli cya gene was analyzed by using S1 nuclease mapping and in vitro transcription experiments. The cya gene was transcribed, both in vivo and in vitro, from one major promoter (P2) and two weak promoters (P1 and P1) that are located about 200 base pairs upstream of P2. The transcription from P2 was specifically inhibited by cAMP-CRP (cAMP receptor protein) in vitro. This regulatory mechanism was shown to be physiologically relevant through quantitative analyses of the cya mRNA in intact cells by S1 and dot blot assays. DNase I protection experiments revealed that cAMP-CRP binds to the cya DNA region between +11 and -20, in which a consensus CRP binding sequence is present. Moreover, it was found that cAMP-CRP alters the binding of RNA polymerase to the promoter region, thus inhibiting the transcription of the cya gene.. J. Biol. Chem. 260:3063-3070(1985) [PubMed] [Europe PMC] ...
We have previously described purification and characterization of a nuclear protein, TREF, which interacts specifically with the transcriptional control element, TRA, of the human transferrin receptor (TR) gene. In this report we show that TREF can be separated into two functionally distinct DNA-binding activities. The first DNA-binding activity (TRAC) is highly specific for the 8-bp element TRA and the related Escherichia coli cAMP receptor binding site. This motif is homologous to the phorbol 12-tetradecanoate 13-acetate- and cAMP-responsive elements of eukaryotic genes and the regulatory proximal sequence elements of the U1 small nuclear RNA gene and is also present in the promoter of the Drosophila melanogaster yolk protein factor 1 gene. In striking contrast, the second activity exhibits high affinity for the ends of double-stranded DNA in a sequence-unspecific manner and is attributable to the heterodimeric Ku autoantigen. Notably, transcription of Ku is induced during mid-late G0/G1 with ...
Sigma-Aldrich offers abstracts and full-text articles by [Ritu Sharma, Anisha Zaveri, Jayashree Gopalakrishnapai, Thiruneelakantan Srinath, Srinath Thiruneelakantan, Umesh Varshney, Sandhya S Visweswariah].
When creating a LACP Link Aggregation, all Force10 switches (with the exception of the I/O Aggregator) have two transmit timer values. This value determines how often the ports send LACPDUs. The default is one LACPDU sent every 1 second. You can also configure it to send one every 30 seconds with the lacp long-timeout command. Long-timeout is best practice and recommended for the Force10 switches.. When the LACPDU transmit timer values mismatch, this can cause intermittent ungrouping of ports, thus causing connection issues. The following command and output helps determine how many LACPDUs being sent and received. This example uses LACP 10.. FTOS#clear lacp 10 counters. FTOS#show lacp 10 counters. ----------------------------------------------------------------------. LACP PDU Marker PDU Unknown Illegal. Port Xmit Recv Xmit Recv Pkts Rx Pkts Rx. ----------------------------------------------------------------------. Gi 0/1 4560 152 0 0 0 0. Gi 0/3 4560 152 0 0 0 0. As you can see, Xmit ...
Sharma, Ritu and Zaveri, Anisha and Gopalakrishnapai, Jayashree and Thiruneelakantan, Srinath and Thiruneelakantan, Umesh and Visweswariah, Sandhya S (2014) Paralogous cAMP Receptor Proteins in Mycobacterium smegmatis Show Biochemical and Functional Divergence. In: BIOCHEMISTRY, 53 (49). pp. 7765-7776. ...
Nucleic acids encoding mammalian, e.g., human receptors, purified receptor proteins and fragments thereof. Antibodies, both polyclonal and monoclonal, are also provided. Methods of using the compositi
filtered_set; other; UniRef90: No significant hits (1e-5); maizesequence.org: GO:0007186; G-protein coupled receptor protein signaling pathway , GO:0016021; integral to ...
Immunoglobulin E (IgE), directed against components of Acacia (wattle) pollen, has been detected by (RAST) in the sera of some children and adults who develop allergic symptoms in the presence of flowering Acacia trees in Australia. All these subjects also had high levels of IgE directed against Lolium perenne (rye grass) pollen. Inhibition by RAST showed that most of the IgE molecules which bound to Acacia pollen components also bound to L. perenne pollen extracts, and to Glycoprotein 1, the major allergen of L. perenne pollen. In these assays, the allergens have been immobilized on polyvinyl chloride microtitre trays: the sensitivity of this approach is compared to that of commercial RAST kits [3]. Sensitization to gum arabic carbohydrate structures occurs casually in atopic patients with pollen sensitization without obvious exposure to gum arabic. According to the study of Sander I, et al (2006) allergy to gum arabic is mediated preferentially by IgE antibodies directed to polypeptide chains ...
A transcriptional regulator in prokaryotes which, when activated by binding Cyclic AMP, acts at several promoters. Cyclic AMP Receptor protein was originally identified as a catabolite Gene activator protein. It was subsequently shown to regulate several functions unrelated to catabolism, and to be both a negative and a positive regulator of transcription. Cell surface Cyclic AMP Receptors are not included (Cyclic AMP Receptors), nor are the eukaryotic cytoplasmic Cyclic AMP Receptor Proteins, which are the regulatory subunits of Cyclic AMP-Dependent Protein Kinases ...
The genome of Mycobacterium tuberculosis H37Rv includes a homologue of the CRP/FNR (cAMP receptor protein/fumarate and nitrate reduction regulator) family of transcription regulators encoded by Rv3676. Sequencing of the orthologous gene from attenuated Mycobacterium bovis Bacille Calmette-Guérin (BCG) strains revealed point mutations that affect the putative DNA-binding and cNMP-binding domains of the encoded protein. These mutations are not present in the published sequences of the Rv3676 orthologues in M. bovis, M. tuberculosis or Mycobacterium leprae. An Escherichia coli lacZ reporter system was used to show that the M. tuberculosis Rv3676 protein binds to DNA sites for CRP, but this DNA binding was decreased or abolished with the Rv3676 protein counterparts from BCG strains. The DNA-binding ability of the M. tuberculosis Rv3676 protein was decreased by the introduction of base changes corresponding to the BCG point mutations. Conversely, the DNA binding of the BCG Rv3676 proteins from BCG strains
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Initialize a given class with the message bundle Keys Should be called from a class that extends NLS in a static block at class load time ...
have a virus i think, headache dizzy hot and cold etc and my god im tempted to eat crap i only had wi at class last night and had my usual treat tea...
Phototaxis and thermotaxis by Dictyostelium discoideum slugs on water agar were impaired by the presence in the agar of adenosine, which is a cyclic AMP receptor antagonist in aggregating amoebae. Caffeine, and presumably its analogue theophylline, inhibit cyclic AMP signalling in aggregating amoebae of D. discoideum. Both compounds perturbed slug behaviour in a similar manner to adenosine, as did both ammonium and sulphate ions. (NH4)3SO4 is known to perturb cyclic AMP binding to its receptor, and ammonia is an inhibitor of cyclic AMP signalling in aggregating amoebae. The receptor agonist, cyclic AMPS, disrupted slug organization and impaired phototaxis when present at concentrations high enough to saturate cyclic AMP receptors and compete effectively with endogenous cyclic AMP signals of similar magnitude to those observed during aggregation. Taken together with the considerable circumstantial evidence for cyclic AMP signalling in slugs, these results support a role for cyclic AMP signalling ...
Bacteria sense the amount of glucose by watching the product of another chemical reaction. The enzyme adenylate cyclase converts ATP into cAMP (cyclic adenosine monophosphate). The cAMP acts as a messenger to the cell ... it says "Im starving". How does it do that? It turns out that when glucose levels are sufficient, adenylate cyclase activity is inhibited. Glucose interferes with the production of cAMP. Thus, when glucose is high, cAMP is low and vice-versa. cAMP activates the protein CAP (sometimes called CRP, for "cAMP Receptor Protein"). When CAP-cAMP binds to the promoter of the lac operon, it revs up transcription to a very high level. If lactose is absent, repression prevents transcription ... but if lactose is present, the receptor protein is deactivated and you get tonnes of permease and beta-galactosidase (and transacetylase, whatever thats for ...). ...
Porcine C-Reactive Protein/CRP ELISA Kit (Colorimetric). High sensitivity ELISA kit for detection of C-Reactive Protein/CRP. Backed by our 100% Guarantee.
The Buoyancy module is key to both the Degradation and the Aggregation systems. Buoyancy is required to position our bacteria in the water column, and also to enable them to buoy the plastic aggregates. This module requires driving the expression of a gas vesicle gene cluster. Gas vesicles are formed within the cell, and are hollow spaces surrounded by a wall of hydrophobic protein. These gas vesicles are permeable to gases, which diffuse into the gas vesicles, increasing its partial pressure, thereby increasing buoyancy. The Buoyancy system is subject to the control of a glucose-repressible promoter, cstA (BBa_K118011). During carbon starvation, ATP is transformed into cAMP and then it binds the cAMP receptor protein, this complex activates the cstA promoter. Studies suggest that cstA promoter can be used to induce the expression of reporter genes in cultures with different glucose concentrations (Schultz and Matin, 1990). Free glucose concentration varies in the upper 300 m of the seawater ...
The Buoyancy module is key to both the Degradation and the Aggregation systems. Buoyancy is required to position our bacteria in the water column, and also to enable them to buoy the plastic aggregates. This module requires driving the expression of a gas vesicle gene cluster. Gas vesicles are formed within the cell, and are hollow spaces surrounded by a wall of hydrophobic protein. These gas vesicles are permeable to gases, which diffuse into the gas vesicles, increasing its partial pressure, thereby increasing buoyancy. The Buoyancy system is subject to the control of a glucose-repressible promoter, cstA (BBa_K118011). During carbon starvation, ATP is transformed into cAMP and then it binds the cAMP receptor protein, this complex activates the cstA promoter. Studies suggest that cstA promoter can be used to induce the expression of reporter genes in cultures with different glucose concentrations (Schultz and Matin, 1990). Free glucose concentration varies in the upper 300 m of the seawater ...
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CRP may refer to: C-reactive protein, an acute phase protein produced by the liver cAMP receptor protein (catabolite gene activator protein), a regulatory protein in bacteria Carbon-fiber-reinforced polymer, an extremely strong and light fiber-reinforced polymer Chinese restaurant process, a partition-valued stochastic process in probability theory Chronic relapsing polyneuropathy, an acquired immune-mediated inflammatory disorder of the peripheral nervous system Confluent and reticulated papillomatosis, a rare skin condition involving pigmentation Common reuse principle, in software design Conference room pilot, in software validation Calgary Regional Partnership, a cooperative of economically associated municipalities in Alberta, Canada California Republican Party, an affiliate of the United States Republican Party Chicana Rights Project, a legal project to protect Mexican American womens rights Committee to Re-elect the President, a fundraising organization of Richard Nixons administration, ...
2 References. Quantikine Mouse C-Reactive Protein/CRP ELISA Kit(MCRP00). Sensitivity: 0.015 ng/mL. Validated for Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma. High precision and reproducibility. View product details.
Townsend, P. D., Jungwirth, B., Pojer, F., Bußmann, M., Money, V. A., Cole, S. T., Pühler, A., et al. (2014). The Crystal Structures of Apo and cAMP-Bound GlxR from Corynebacterium glutamicum Reveal Structural and Dynamic Changes upon cAMP Binding in CRP/FNR Family Transcription Factors. PloS one, 9(12), 113265. doi:10.1371/journal.pone.0113265 ...
Members of Princetons class of 2009 gathered on Cannon Green Monday, June 1, to celebrate the conclusion of their undergraduate careers in a Class Day ceremony honoring their service and accomplishments.
Tuesdays are one of my most favorite days - I get out of school just an hour or two after lunch, and on days we dont have Surgery lab, I dont have to be at class until 9. But now, its 9:30PM and I am exhausted. Heres a rundown of my productive day: 5:10AM: Up,…
CRP se određuje kada postoji sumnja na zapaljenje, kod praćenja efikasnosti terapije i kod praćenja napredovanja nekih bolesti.
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Get the &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;a href="http://www.widgetbox.com/widget/welcome-clock-widgia"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;welcome ...
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A high level of CRP in the blood is a marker of inflammation, which can indicate infection or a chronic inflammatory disease, such as rheumatoid arthritis or lupus, as well as the risk of heart disease.
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Horse Transferrin receptor protein 1 (TFRC) ELISA Kit datasheet and description hight quality product and Backed by our Guarantee
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CRP Antigen. C reactive protein (CRP), also known as PTX1, is encoded by a gene that belongs to the pentaxin family. This protein is involved in several host defense related functions. For instance, CRP has the ability to recognize foreign pathogens and damaged host cells and to initiate their elimination by interacting with the bloods humoral and cellular effector systems. Consequently, the level of this protein in plasma increases greatly during the acute phase response to tissue injury, infection, or other inflammatory stimuli.. [Provided by RefSeq, Sep 2009]. ...
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Phys.org internet news portal provides the latest news on science including: Physics, Space Science, Earth Science, Health and Medicine
Phys.org internet news portal provides the latest news on science including: Physics, Space Science, Earth Science, Health and Medicine
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Ущелье&nbsp;Джеты-Огуз&nbsp;или Скалы Семи Быков (кирг. Жети-Өгүз) - живописное горное ущелье в Киргизии в 28 км&nbsp;на запад от&nbsp...
Ущелье&nbsp;Джеты-Огуз&nbsp;или Скалы Семи Быков (кирг. Жети-Өгүз) - живописное горное ущелье в Киргизии в 28 км&nbsp;на запад от&nbsp...
9,000 남자의 큰 연구 결과는 적합하게 측정된 분석실험에 적용될 때 효과적으로 임상의를 내분비 사회의 임상 내분비학 & 물질 대사의 전표에서 간행된 새로운 연구 결과에 따라 성기능 부전의 정확한 진단을, 만드는 가능하게 할 남자에 있는 총 테스토스테론을 위한 화합한 참조 범위를 설치했습니다
The term `quorum sensing (QS) is used to define a population density based communication mechanism which uses chemical signal molecules called autoinducers to trigger unique and varied changes in gene expression. Although several communication methods have been identified in bacteria that are unique to a particular species, one type of signal molecule, autoinducer-2 (AI-2) is linked to interspecies communication, indicating its potential as a universal signal for cueing a QS response among multiple bacterial types. In E. coli, AI-2 acts as an effector by binding to the QS repressor LsrR. As a result, LsrR unbinds and relieves repression of the lsr regulon, stimulating a subsequent QS gene expression cascade. In this dissertation, LsrR structure and in vitro binding activity are examined. Genomic binding and DNA microarray analyses are conducted and three novel sites putatively regulated by LsrR, yegE-udk, mppA and yihF, are revealed. Two cAMP receptor protein (CRP) binding locations in ...
C-reactive protein (CRP) is an acute-phase reactant frequently used in histochemistry like a marker of ongoing inflammation. [AA] 218 to 232) was identified by CP-724714 monoclonal antibodies CRP-8 and 9C9. This epitope region of Hsp60 displays 26.6% amino acid identity to CRP AA region 77 to 90. These data suggest that the B-cell epitopes shared between CRP and Hsp60 give rise to a true mimicry-based cross-reaction and the induction of cross-reactive antibodies. Our study underlines the importance of thorough study design and careful interpretation of results while using polyclonal anti-CRP antibodies for histochemistry, especially at low dilutions. Furthermore, analytical interference with Hsp60 in CRP assays should also become tested. C-reactive protein (CRP) is an acute-phase reactant in humans, rabbits, and a number of additional mammalian varieties. It is indicated and secreted primarily by hepatocytes, but recently, local production at sites of swelling by monocytes has also been reported ...
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amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;div style="display:inline;"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;img height="1" width="1" style="border-style:none;" alt="" src="//googleads.g.doubleclick.net/pagead/viewthroughconversion/863606681/?guid=ON&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;script=0"/&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;/div&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt ...
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amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;a href="http://rafl.es/enable-js"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;You need javascript enabled to see this giveaway&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;/a&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt ...
There is no special preparation for a C-reactive protein (CRP) test. You might be asked to not eat or drink for a few hours before the test.. Tell your doctor all of the medicines you are taking because some medicines can affect the results.. Talk to your doctor about any concerns you have regarding the need for the test, its risks, how it will be done, or what the results will mean. To help you understand the importance of this test, fill out the medical test information form ( What is a PDF document? ).. ...
amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;applet codebase="http://ri.revolvermaps.com/j" code="core.RE" width="250" height="250" archive="g.jar"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;param name="cabbase" value="g.cab" /&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;param name="r" value="true" /&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;param name="n" value="false" /&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;param name="i" value="8aoxpeh4ob5" ...
amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;/font&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;/p&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;p style="margin: 0px 0px 15px; padding: 0px; border: 0px; outline: 0px;"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;font style="margin: 0px; padding: 0px; border: 0px; outline: 0px; line-height: 18px;" face="Georgia, serif"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt; Share &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;a href="http://www.goodreads.com/"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;book ...
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amp;amp;amp;amp;amp;amp;amp;amp;lt;span itemprop="name" content="Why ending violence is a development imperative"&amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;lt;/span&amp;amp;amp;amp;amp;amp;amp;gt; &amp;amp;amp;amp;amp;amp;amp;lt;span itemprop="description" content=""&amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;lt;/span&amp;amp;amp;amp;amp;amp;amp;gt; &amp;amp;amp;amp;amp;amp;amp;lt;span itemprop="duration" content="333"&amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;lt;/span&amp;amp;amp;amp;amp;amp;amp;gt; &amp;amp;amp;amp;amp;amp;amp;lt;span itemprop="thumbnail" content="https://cfvod.kaltura.com/p/619672/sp/61967200/thumbnail/entry_id/1_747uficf/version/100011/acv/211"&amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;lt;/span&amp;amp;amp;amp;amp;amp;amp;gt; &amp;amp;amp;amp;amp;amp;amp;lt;span itemprop="width" ...
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lt;p>&amp;amp;amp;lt;p&amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;lt;p&amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;p&amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;a href="http://video.news.com.au/2227300814/Shorten-supports-PM-on-Slipper"&amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;VIDEO: Shorten supports PM on Slipper&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;/a&amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;/p&amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;lt;/p&amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;lt;/p&amp;amp;amp;gt;</p> ...
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1. Noradrenaline and histamine, when added to superfused guinea-pig cerebral-cortical tissues, increased both cyclic AMP-dependent and -independent histone kinase activities of some, but not of all, subsequently isolated subcellular fractions, and decreased their cyclic [3H]AMP-binding capacity, which was concluded to be due to an increase in endogenously bound cyclic AMP. 2. Adenosine and 2-chloroadenosine also diminished the cyclic [3H]AMP-binding capacities, but did not affect the histone kinase activities. 3. DEAE-cellulose chromatography and stability to KCl additions showed that the greater part of the histone kinase of the present preparations corresponded to the type II enzyme [of Corbin, Keely & Park (1975) J. Biol. Chem. 250, 218-225], with a lesser amount of type I activity. Different sites of cyclic AMP accumulation in relation to these or other kinases are considered in interpreting the differential tissue responses to the neurohumoural agents examined.. ...
The ada and aidB genes are part of the adaptive response to DNA methylation damage in Escherichia coli. Transcription of the ada and the aidB genes is triggered by binding of the methylated Ada protein (meAda) to a specific sequence located 40-60 base pairs upstream of the transcriptional start, which is internal to an A/T-rich region. In this report we demonstrate that the Ada binding site is also a binding site for RNA polymerase. RNA polymerase is able to bind the -40 to -60 region of the ada and the aidB promoters in the absence of meAda, and its binding is mediated by the alpha subunit. This region resembles the UP element of the rrnB P1 promoter in location, sequence and mechanism of interaction with RNA polymerase. We discuss the function of UP-like elements in positively controlled promoters and provide evidence that Ada does not act by enhancing RNA polymerase binding affinity to the promoter region. Instead, Ada stimulates transcription by modifying the nature of the RNA polymerase-promoter
amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;a href="http://adserver.adtech.de/adlink,3.0,1703.1,5928369,0,6792,ADTECH;loc=300;alias=" target="_blank"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;img src="http://adserver.adtech.de/adserv,3.0,1703.1,5928369,0,6792,ADTECH;loc=300;alias=" border="0" width="640" height="102"&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt;/a&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt ...

Transcription activation at Escherichia coli promoters dependent on the cyclic AMP receptor protein: effects of binding...Transcription activation at Escherichia coli promoters dependent on the cyclic AMP receptor protein: effects of binding...

Transcription activation at Escherichia coli promoters dependent on the cyclic AMP receptor protein: effects of binding ... Transcription activation at Escherichia coli promoters dependent on the cyclic AMP receptor protein: effects of binding ... Transcription activation at Escherichia coli promoters dependent on the cyclic AMP receptor protein: effects of binding ... Transcription activation at Escherichia coli promoters dependent on the cyclic AMP receptor protein: effects of binding ...
more infohttp://www.biochemj.org/content/309/1/77

Transcriptional Regulation of Vibrio cholerae Hemagglutinin/Protease by the Cyclic AMP Receptor Protein and RpoS | Journal of...Transcriptional Regulation of Vibrio cholerae Hemagglutinin/Protease by the Cyclic AMP Receptor Protein and RpoS | Journal of...

The cyclic AMP (cAMP) receptor protein (CRP) strongly enhanced hapA transcription in the deceleration phase. Analysis of rpoS ... Cyclic AMP and its receptor protein negatively regulate the coordinate expression of cholera toxin and toxin co-regulated pilus ... Transcriptional Regulation of Vibrio cholerae Hemagglutinin/Protease by the Cyclic AMP Receptor Protein and RpoS. Anisia J. ... 64 ng of protein (lane 2), 128 ng of protein (lane 3), no protein (lane 4), 64 ng of protein (lane 5), 128 ng of protein (lane ...
more infohttps://jb.asm.org/content/186/19/6374?ijkey=f43dde75cbaced18267e8b02a80103a4a3b52b63&keytype2=tf_ipsecsha

Cyclic AMP Receptor Protein-Dependent Activation of the Escherichia coli acsP2 Promoter by a Synergistic Class III Mechanism |...Cyclic AMP Receptor Protein-Dependent Activation of the Escherichia coli acsP2 Promoter by a Synergistic Class III Mechanism |...

Protein-protein interactions during transcription activation: the case of the Escherichia coli cyclic AMP receptor protein. ... The Escherichia coli cyclic AMP (cAMP) receptor protein (CRP; also known as the catabolite activator protein) activates ... Cyclic AMP Receptor Protein-Dependent Activation of the Escherichia coli acsP2 Promoter by a Synergistic Class III Mechanism. ... The cyclic AMP receptor protein (CRP) activates transcription of the Escherichia coli acs gene, which encodes an acetate- ...
more infohttps://jb.asm.org/content/185/17/5148?ijkey=46771fa22b68d857eac1550cf3372ea042ae1648&keytype2=tf_ipsecsha

Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic...Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic...

Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic ... Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic ... Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic ... Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic ...
more infohttps://iai.asm.org/content/55/12/3035?ijkey=0fe855d75ed2278a60d59480775a93a4ae7d0f61&keytype2=tf_ipsecsha

The Global Regulatory Cyclic AMP Receptor Protein (CRP) Controls Multifactorial Fluoroquinolone Susceptibility in Salmonella...The Global Regulatory Cyclic AMP Receptor Protein (CRP) Controls Multifactorial Fluoroquinolone Susceptibility in Salmonella...

Here we report that loss of the global regulatory protein cyclic AMP (cAMP) receptor protein (CRP) or its allosteric effector, ... These genes encode the global regulatory protein cyclic AMP (cAMP) receptor protein (CRP) and the enzyme adenylate cyclase (Cya ... The Global Regulatory Cyclic AMP Receptor Protein (CRP) Controls Multifactorial Fluoroquinolone Susceptibility in Salmonella ... The Global Regulatory Cyclic AMP Receptor Protein (CRP) Controls Multifactorial Fluoroquinolone Susceptibility in Salmonella ...
more infohttps://aac.asm.org/content/61/11/e01666-17

Error-prone PCR of global transcription factor cyclic AMP receptor protein for enhanced organic solvent (toluene) tolerance |...Error-prone PCR of global transcription factor cyclic AMP receptor protein for enhanced organic solvent (toluene) tolerance |...

Basak, S., Song, H., & Jiang, R. (2012). Error-prone PCR of global transcription factor cyclic AMP receptor protein for ... Error-prone PCR of global transcription factor cyclic AMP receptor protein for enhanced organic solvent (toluene) tolerance. ... improve the organic solvent tolerance of Escherichia coli by engineering its global transcription factor cAMP receptor protein ...
more infohttps://dr.ntu.edu.sg/handle/10220/12336

Evidence For Dissimilar Cyclic-Amp Receptor Protein Binding Sties In E by Mark Schmidt"Evidence For Dissimilar Cyclic-Amp Receptor Protein Binding Sties In E" by Mark Schmidt

While the mutation was not reliably mapped to crp, several lines of evidence indicate the genesis of a second form of c-AMP ... A mutant strain was developed that is independent of the c-AMP requirement for induction of the arabinose operon, but dependent ... Recent work with a mutant CRP independent of the c-AMP requirement for induction of the lactose operon suggested that the CRP ... on c-AMP for induction of the lactose operon. ... Evidence For Dissimilar Cyclic-Amp Receptor Protein Binding ...
more infohttps://scholars.carroll.edu/lifesci_theses/487/

Interplay between site-specific mutations and cyclic nucleotides in modulating DNA recognition by Escherichia coli cyclic AMP...Interplay between site-specific mutations and cyclic nucleotides in modulating DNA recognition by Escherichia coli cyclic AMP...

Mutagenesis of various amino acids in Escherichia coli cyclic AMP receptor protein (CRP) has been shown to modulate protein ... "Mutagenesis of various amino acids in Escherichia coli cyclic AMP receptor protein (CRP) has been shown to modulate protein ... Mutagenesis of various amino acids in Escherichia coli cyclic AMP receptor protein (CRP) has been shown to modulate protein ... Mutagenesis of various amino acids in Escherichia coli cyclic AMP receptor protein (CRP) has been shown to modulate protein ...
more infohttps://researchexperts.utmb.edu/en/publications/interplay-between-site-specific-mutations-and-cyclic-nucleotides-

Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis (pdf) |...Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis (pdf) |...

Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis, BMC ... and six Yop effector proteins including YpkA and YopJ. The cyclic AMP receptor protein (CRP), a global regulator, was recently ... and six Yop effector proteins including YpkA and YopJ. The cyclic AMP receptor protein (CRP), a global regulator, was recently ... The cyclic AMP receptor protein (CRP) is a global regulator that controls the transcription initiation for more than 100 ...
more infohttps://paperity.org/p/57231605/direct-and-negative-regulation-of-the-syco-ypka-ypoj-operon-by-cyclic-amp-receptor

Communications between the High-Affinity Cyclic Nucleotide Binding Sites in
    E. coli
    Cyclic AMP Receptor Protein: ...Communications between the High-Affinity Cyclic Nucleotide Binding Sites in E. coli Cyclic AMP Receptor Protein: ...

Communications between the High-Affinity Cyclic Nucleotide Binding Sites in E. coli Cyclic AMP Receptor Protein: Effect of ...
more infohttps://www.infona.pl/resource/bwmeta1.element.acs-doi-10_1021_bi026099z

Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta...Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta...

... primary role of a beta-adrenergic receptor/cyclic AMP mechanism. Message Subject (Your Name) has forwarded a page to you from ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ... Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta- ...
more infohttp://molpharm.aspetjournals.org/content/47/3/439

Antibiotics  | Free Full-Text | Multiple Pathways of Genome Plasticity Leading to Development of Antibiotic Resistance | HTMLAntibiotics | Free Full-Text | Multiple Pathways of Genome Plasticity Leading to Development of Antibiotic Resistance | HTML

de Crombrugghe, B.; Busby, S.; Buc, H. Cyclic AMP receptor protein: Role in transcription activation. Science 1984, 224, 831- ... Sinha, S.; Mell, J.C.; Redfield, R.J. Seventeen Sxy-dependent cyclic AMP receptor protein site-regulated genes are needed for ... Liang, W.; Pascual-Montano, A.; Silva, A.J.; Benitez, J.A. The cyclic AMP receptor protein modulates quorum sensing, motility ... Kumar, S.; Srivastava, S. Cyclic AMP and its receptor protein are required for expression of transfer genes of conjugative ...
more infohttp://mdpi.com/2079-6382/2/2/288/htm

Frontiers | The Global Acetylome of the Human Pathogen Vibrio cholerae V52 Reveals Lysine Acetylation of Major Transcriptional...Frontiers | The Global Acetylome of the Human Pathogen Vibrio cholerae V52 Reveals Lysine Acetylation of Major Transcriptional...

... we identified 3402 acetylation sites on 1240 proteins. Of the acetylated proteins, more than half were acetylated on two or ... we identified 3402 acetylation sites on 1240 proteins. Of the acetylated proteins, more than half were acetylated on two or ... In conclusion, this is the first global protein lysine acetylome analysis of V. cholerae and should constitute a valuable r... ... As reported for other bacteria, we observed that many of the acetylated proteins were involved in metabolic and cellular ...
more infohttps://www.frontiersin.org/articles/10.3389/fcimb.2017.00537/full

Global regulation of gene expression in Escherichia coli. - Semantic ScholarGlobal regulation of gene expression in Escherichia coli. - Semantic Scholar

Cyclic AMP Receptor Protein Acts as a Transcription Regulator in Response to Stresses in Deinococcus radiodurans. *Su Yang, ... Mutations in the Escherichia coli ribosomal protein L22 selectively suppress the expression of a secreted bacterial virulence ...
more infohttps://www.semanticscholar.org/paper/Global-regulation-of-gene-expression-in-Escherichia-Chuang-Daniels/8d560d5e006f44974064e8b0303457fc9894fa61

Cis-2-dodecenoic acid receptor RpfR links quorum-sensing signal perception with regulation of virulence through cyclic dimeric...Cis-2-dodecenoic acid receptor RpfR links quorum-sensing signal perception with regulation of virulence through cyclic dimeric...

2002) Stoichiometry and structural effect of the cyclic nucleotide binding to cyclic AMP receptor protein. J Biol Chem 277: ... negatively controls the intracellular cyclic dimeric guanosine monophosphate (c-di-GMP) level through a receptor protein RpfR, ... upon which the signal activates its cognate receptor, a LuxR-family protein, by the formation of a protein-ligand complex, ... In this study we identified the unique BDSF receptor RpfR, a multidomain protein containing a PAS, a GGDEF, and an EAL domain ( ...
more infohttp://www.pnas.org/content/109/38/15479

Frontiers | Cold Shock Proteins: A Minireview with Special Emphasis on Csp-family of Enteropathogenic Yersinia | MicrobiologyFrontiers | Cold Shock Proteins: A Minireview with Special Emphasis on Csp-family of Enteropathogenic Yersinia | Microbiology

Moreover, protein folding is inefficient and ribosome function is hampered. Csps are thought to counteract these harmful ... Moreover, protein folding is inefficient and ribosome function is hampered. Csps are thought to counteract these harmful ... Many bacteria produce small cold shock proteins (Csp) as a response to rapid temperature downshift (cold shock). During cold ... Many bacteria produce small cold shock proteins (Csp) as a response to rapid temperature downshift (cold shock). During cold ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2016.01151/full

Cancer Institute, Mechanisms of Carcinogenesis - Research Output
     - Penn StateCancer Institute, Mechanisms of Carcinogenesis - Research Output - Penn State

A dynamic role for the Ah receptor in cell signaling? Insights from a diverse group of Ah receptor interacting proteins. ... Aryl hydrocarbon receptor ligands in cancer: Friend and foe. Murray, I. A., Patterson, A. D. & Perdew, G. H., Nov 24 2014, In ... Biology of the PPAR family of receptors. Vanden Heuvel, J. P. & Peters, J. M., 2001, In : Comments on Toxicology. 7, 3, p. 233- ... Xiong, N., Fu, Y., Hu, S., Xia, M. & Yang, J., Aug 2012, In : Protein and Cell. 3, 8, p. 571-580 10 p.. Research output: ...
more infohttps://pennstate.pure.elsevier.com/en/organisations/cancer-institute-mechanisms-of-carcinogenesis/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Fsystematicreview&ordering=type&descending=false

Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3...Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3...

... two dominant CCR mechanisms involve transcriptional regulation by cyclic AMP receptor protein (Crp) and by catabolite repressor ... ScrA EII transport protein for sucrose, ScrB sucrose-6-phosphate hydrolase, ScrK fructokinase, PtsG EIIBCGlc complex, FruA ... Gao L, Chi Z, Sheng J, Ni X, Wang L (2007) Single-cell protein production from Jerusalem artichoke extract by a recently ... Ebner R, Lengeler JW (1988) DNA-sequence of the gene scrA encoding the sucrose transport protein Enzymellscr of the ...
more infohttps://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0290-3

Molecular cloning and transcriptional regulation of ompT, a ToxR-repressed gene in Vibrio cholerae.  - PubMed - NCBIMolecular cloning and transcriptional regulation of ompT, a ToxR-repressed gene in Vibrio cholerae. - PubMed - NCBI

Promoter fusion studies also suggest that cyclic AMP receptor protein (CRP) is involved in ompT activation. Gel mobility shift ... OmpT is the only identified protein under negative regulation of ToxR. To understand the molecular mechanism by which ToxR ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10632889?dopt=Abstract

Bose JL[au] - PubMed - NCBIBose JL[au] - PubMed - NCBI

Cyclic AMP receptor protein regulates pheromone-mediated bioluminescence at multiple levels in Vibrio fischeri ES114. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Bose+JL%5Bau%5D&dispmax=50

Analysis of Global Transcriptional Profiles of Enterotoxigenic Escherichia coli Isolate E24377A | Infection and ImmunityAnalysis of Global Transcriptional Profiles of Enterotoxigenic Escherichia coli Isolate E24377A | Infection and Immunity

Cyclic AMP receptor protein-dependent repression of heat-labile enterotoxin. Infect. Immun. 77:791-798. ... The tib adherence locus of enterotoxigenic Escherichia coli is regulated by cyclic AMP receptor protein. J. Bacteriol. 193:1369 ... which elevates intracellular cyclic AMP (cAMP) that then activates the cystic fibrosis transmembrane regulator (CFTR) chloride ... Enterotoxigenic Escherichia coli elicits immune responses to multiple surface proteins. Infect. Immun. 78:3027-3035. ...
more infohttps://iai.asm.org/content/80/3/1232?ijkey=816a22a18c39feecf92ee3c3bca22ef7d45fb0d9&keytype2=tf_ipsecsha

The hierarchy of regulation network of CRP.A total of 7 | Open-iThe hierarchy of regulation network of CRP.A total of 7 | Open-i

Cyclic AMP Receptor Protein/chemistry/metabolism*. *Escherichia coli Proteins/chemistry/metabolism*. Minor ... CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best ... CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best ... Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources. ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3105977_pone.0020081.g006&req=4

Molecular mechanism of SugR-mediated sugar-dependent expression of the ldhA gene encoding l-lactate dehydrogenase in...Molecular mechanism of SugR-mediated sugar-dependent expression of the ldhA gene encoding l-lactate dehydrogenase in...

... as a protein binding to the ldhA promoter region. Whereas ldhA gene expression and ldhA promoter activity were completely ... Genome-wide identification of in vivo binding sites of GlxR, a cyclic AMP receptor protein-type regulator in Corynebacterium ... as a protein binding to the ldhA promoter region. Whereas ldhA gene expression and ldhA promoter activity were completely ...
more infohttps://www.semanticscholar.org/paper/Molecular-mechanism-of-SugR-mediated-expression-of-Toyoda-Teramoto/218d5b7793f8f2dd2fc8567299df4b9b7d1a12a0

Rearrangements of 2.5 Kilobases of Noncoding DNA from the  Locus Define Predictive Rules of Genomic -Regulatory Logic |...Rearrangements of 2.5 Kilobases of Noncoding DNA from the Locus Define Predictive Rules of Genomic -Regulatory Logic |...

The binding specificity of cyclic AMP receptor protein to recognition sites. Journal of Molecular Biology 200: 709-723. ... Once we have calculated , we calculate the effects of protein-protein interactions. A TF bound at site acting on a TF bound at ... The scale of relative fluorescence levels for RNA is shown at the left of graphs, that for proteins on the right. All protein ... 3. Model equations: protein-protein interactions. The model equations for coactivation (Equation 5), short-range quenching ( ...
more infohttps://www.prolekare.cz/casopisy/plos-genetics/2013-2/rearrangements-of-2-5-kilobases-of-noncoding-dna-from-the-locus-define-predictive-rules-of-genomic-regulatory-logic-43892
  • Structural information was presented to rationalize the interplay between CRP sequence and cyclic nucleotides in defining the recognition of DNA sequences. (utmb.edu)
  • Results showed that protein banding patterns in both the cytoplasmic fraction and in a fraction enriched in chromatin-bound proteins showed some individual variability in tissues of different animals, but exhibited no changes that can be attributed to the flight. (nasa.gov)
  • While the mutation was not reliably mapped to crp, several lines of evidence indicate the genesis of a second form of c-AMP independent CRP selected for inducing ara. (carroll.edu)
  • Some nucleoid proteins play not only architectural roles but also regulatory roles (blue-green background). (nih.gov)
  • Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: primary role of a beta-adrenergic receptor/cyclic AMP mechanism. (aspetjournals.org)
  • This antiserum generates a supershifted CREB signal with protein extracts obtained from glands treated with NE (EC50 approximately equal to 10 nM) in organ culture, demonstrating that NE stimulates CREB phosphorylation. (aspetjournals.org)
  • Data from such experiments show that PKA increases the phosphorylation of several microsac proteins, including a 66-kDa polypeptide specifically immunoprecipitated with the GABAA receptor anti-alpha 1 subunit antibody. (muscimol.xyz)
  • These results provide evidence that the catalytic subunit of PKA inhibits the function of brain GABAA receptors and demonstrate that this functional change is concomitant with an increase in protein phosphorylation. (muscimol.xyz)
  • An integrated view of the roles and mechanisms of heat shock protein gp96-peptide complex in eliciting immune response. (elsevier.com)
  • Two proteins needed to utilize lactose (permease, required for the entry of lactose into the cell, and β -galactosidase, the enzyme that cleaves lactose) are tightly linked and transcribed into a single poly- cistronic mRNA molecule, lac mRNA. (coursehero.com)
  • Hence it stops biosynthesis if guanine can be obtained from the external medium, increases its expression if nucleotides are needed(for example during DNA replication) and balances the production of GMP with respect to AMP and the pyrimidine nucleotides. (wikipedia.org)
  • In conclusion, this is the first global protein lysine acetylome analysis of V. cholerae and should constitute a valuable resource for in-depth studies of the impact of lysine acetylation in pathogenesis and other cellular processes. (frontiersin.org)
  • Photoaffinity labeling with a 32P-8-azido analog of cyclic AMP, and electrophoretic separation of the proteins, was followed by autoradiographic identification of the subcellular fraction in which the labeled R subunits are localized. (nasa.gov)
  • In parallel experiments, PKA and [gamma-32P]ATP were introduced into the microsacs, and we attempted to immunoprecipitate the entire GABAA receptor complex, under nondenaturing conditions, using an anti-alpha 1-subunit antibody. (muscimol.xyz)
  • This dual control ensures that there is a balance of flux between AMP and GMP and the flux partition remains relatively constant even in the face of perturbations. (wikipedia.org)
  • The P. multocida crp mutant exhibited a similar lipopolysaccharide and outer membrane protein profile but displayed defective growth and serum complement resistance in vitro compared with the parent strain. (biomedcentral.com)
  • All mutations altered the surface chemistry of CRP as evidenced by the change in elution properties of these proteins from different matrixes. (utmb.edu)
  • As yet, a precise and predictive decryption of this code comparable to the genetic code for protein structure has remained elusive. (prolekare.cz)