Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
The rate dynamics in chemical or physical systems.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues of proteins and is dependent on cyclic nucleotides.
A group of cyclic GMP-dependent enzymes that catalyze the phosphorylation of SERINE or THREONINE residues of proteins.
Guanosine cyclic 3',5'-(hydrogen phosphate). A guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. It is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. (From Merck Index, 11th ed)
A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)
Dammarane type triterpene saponins based mainly on the aglycones, protopanaxadiol and protopanaxatriol.
An araliaceous genus of plants that contains a number of pharmacologically active agents used as stimulants, sedatives, and tonics, especially in traditional medicine. Sometimes confused with Siberian ginseng (ELEUTHEROCOCCUS).
A plant species of the genus PANAX. It contains damarane-type tetracyclic TRITERPENES. The common names of Sanchi or Tienchi are also used for Panax pseudoginseng which is distinguished in containing oleanane-type pentacyclic triterpenoids.
A type of glycoside widely distributed in plants. Each consists of a sapogenin as the aglycone moiety, and a sugar. The sapogenin may be a steroid or a triterpene and the sugar may be glucose, galactose, a pentose, or a methylpentose.
The aglucon moiety of a saponin molecule. It may be triterpenoid or steroid, usually spirostan, in nature.
A cyclic GMP-dependent protein kinase subtype that is expressed in SMOOTH MUSCLE tissues and plays a role in regulation of smooth muscle contraction. Two isoforms, PKGIalpha and PKGIbeta, of the type I protein kinase exist due to alternative splicing of its mRNA.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
A cyclic GMP-dependent protein kinase subtype that is expressed predominantly in INTESTINES, BRAIN, and KIDNEY. The protein is myristoylated on its N-terminus which may play a role its membrane localization.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A cyclic AMP-dependent protein kinase subtype primarily found in particulate subcellular fractions. They are tetrameric proteins that contain two catalytic subunits and two type II-specific regulatory subunits.
An important regulator of GENE EXPRESSION during growth and development, and in NEOPLASMS. Tretinoin, also known as retinoic acid and derived from maternal VITAMIN A, is essential for normal GROWTH; and EMBRYONIC DEVELOPMENT. An excess of tretinoin can be teratogenic. It is used in the treatment of PSORIASIS; ACNE VULGARIS; and several other SKIN DISEASES. It has also been approved for use in promyelocytic leukemia (LEUKEMIA, PROMYELOCYTIC, ACUTE).
A group of tetraterpenes, with four terpene units joined head-to-tail. Biologically active members of this class are used clinically in the treatment of severe cystic ACNE; PSORIASIS; and other disorders of keratinization.

Intracellular signalling: PDK1--a kinase at the hub of things. (1/7238)

Phosphoinositide-dependent kinase 1 (PDK1) is at the hub of many signalling pathways, activating PKB and PKC isoenzymes, as well as p70 S6 kinase and perhaps PKA. PDK1 action is determined by colocalization with substrate and by target site availability, features that may enable it to operate in both resting and stimulated cells.  (+info)

The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579. (2/7238)

The extracellular receptor stimulated kinase ERK2 (p42(MAPK))-phosphorylated human cAMP-specific phosphodiesterase PDE4D3 at Ser579 and profoundly reduced ( approximately 75%) its activity. These effects could be reversed by the action of protein phosphatase PP1. The inhibitory state of PDE4D3, engendered by ERK2 phosphorylation, was mimicked by the Ser579-->Asp mutant form of PDE4D3. In COS1 cells transfected to express PDE4D3, challenge with epidermal growth factor (EGF) caused the phosphorylation and inhibition of PDE4D3. This effect was blocked by the MEK inhibitor PD98059 and was not apparent using the Ser579-->Ala mutant form of PDE4D3. Challenge of HEK293 and F442A cells with EGF led to the PD98059-ablatable inhibition of endogenous PDE4D3 and PDE4D5 activities. EGF challenge of COS1 cells transfected to express PDE4D3 increased cAMP levels through a process ablated by PD98059. The activity of the Ser579-->Asp mutant form of PDE4D3 was increased by PKA phosphorylation. The transient form of the EGF-induced inhibition of PDE4D3 is thus suggested to be due to feedback regulation by PKA causing the ablation of the ERK2-induced inhibition of PDE4D3. We identify a novel means of cross-talk between the cAMP and ERK signalling pathways whereby cell stimuli that lead to ERK2 activation may modulate cAMP signalling.  (+info)

Phosphorylation of the DNA repair protein APE/REF-1 by CKII affects redox regulation of AP-1. (3/7238)

The DNA repair protein apurinic endonuclease (APE/Ref-1) exerts several physiological functions such as cleavage of apurinic/apyrimidinic sites and redox regulation of the transcription factor AP-1, whose activation is part of the cellular response to DNA damaging treatments. Here we demonstrate that APE/Ref-1 is phosphorylated by casein kinase II (CKII). This was shown for both the recombinant APE/Ref-1 protein (Km=0.55 mM) and for APE/Ref-1 expressed in COS cells. Phosphorylation of APE/Ref-1 did not alter the repair activity of the enzyme, whereas it stimulated its redox capability towards AP-1, thus promoting DNA binding activity of AP-1. Inhibition of CKII mediated phosphorylation of APE/Ref-1 blocked mutagen-stimulated increase in AP-1 binding. It also abrogated the induction of c-Jun protein and rendered cells more sensitive to induced DNA damage. Thus, phosphorylation of APE/Ref-1 appears to be involved in regulating the different physiological activities of the enzyme. CKII mediated phosphorylation of APE/Ref-1 and concomitant increase in AP-1 binding activity appears to be a novel mechanism of cellular stress response, forcing transcription of AP-1 target gene(s) the product(s) of which may exert protective function.  (+info)

A novel interaction mechanism accounting for different acylphosphatase effects on cardiac and fast twitch skeletal muscle sarcoplasmic reticulum calcium pumps. (4/7238)

In cardiac and skeletal muscle Ca2+ translocation from cytoplasm into sarcoplasmic reticulum (SR) is accomplished by different Ca2+-ATPases whose functioning involves the formation and decomposition of an acylphosphorylated phosphoenzyme intermediate (EP). In this study we found that acylphosphatase, an enzyme well represented in muscular tissues and which actively hydrolyzes EP, had different effects on heart (SERCA2a) and fast twitch skeletal muscle SR Ca2+-ATPase (SERCA1). With physiological acylphosphatase concentrations SERCA2a exhibited a parallel increase in the rates of both ATP hydrolysis and Ca2+ transport; in contrast, SERCA1 appeared to be uncoupled since the stimulation of ATP hydrolysis matched an inhibition of Ca2+ pump. These different effects probably depend on phospholamban, which is associated with SERCA2a but not SERCA1. Consistent with this view, the present study suggests that acylphosphatase-induced stimulation of SERCA2a, in addition to an enhanced EP hydrolysis, may be due to a displacement of phospholamban, thus to a removal of its inhibitory effect.  (+info)

PrKX is a novel catalytic subunit of the cAMP-dependent protein kinase regulated by the regulatory subunit type I. (5/7238)

The human X chromosome-encoded protein kinase X (PrKX) belongs to the family of cAMP-dependent protein kinases. The catalytically active recombinant enzyme expressed in COS cells phosphorylates the heptapeptide Kemptide (LRRASLG) with a specific activity of 1.5 micromol/(min.mg). Using surface plasmon resonance, high affinity interactions were demonstrated with the regulatory subunit type I (RIalpha) of cAMP-dependent protein kinase (KD = 10 nM) and the heat-stable protein kinase inhibitor (KD = 15 nM), but not with the type II regulatory subunit (RIIalpha, KD = 2.3 microM) under physiological conditions. Kemptide and autophosphorylation activities of PrKX are strongly inhibited by the RIalpha subunit and by protein kinase inhibitor in vitro, but only weakly by the RIIalpha subunit. The inhibition by the RIalpha subunit is reversed by addition of nanomolar concentrations of cAMP (Ka = 40 nM), thus demonstrating that PrKX is a novel, type I cAMP-dependent protein kinase that is activated at lower cAMP concentrations than the holoenzyme with the Calpha subunit of cAMP-dependent protein kinase. Microinjection data clearly indicate that the type I R subunit but not type II binds to PrKX in vivo, preventing the translocation of PrKX to the nucleus in the absence of cAMP. The RIIalpha subunit is an excellent substrate for PrKX and is phosphorylated in vitro in a cAMP-independent manner. We discuss how PrKX can modulate the cAMP-mediated signal transduction pathway by preferential binding to the RIalpha subunit and by phosphorylating the RIIalpha subunit in the absence of cAMP.  (+info)

Prior exposure to neurotrophins blocks inhibition of axonal regeneration by MAG and myelin via a cAMP-dependent mechanism. (6/7238)

MAG is a potent inhibitor of axonal regeneration. Here, inhibition by MAG, and myelin in general, is blocked if neurons are exposed to neurotrophins before encountering the inhibitor; priming cerebellar neurons with BDNF or GDNF, but not NGF, or priming DRG neurons with any of these neurotrophins blocks inhibition by MAG/myelin. Dibutyryl cAMP also overcomes inhibition by MAG/myelin, and cAMP is elevated by neurotrophins. A PKA inhibitor present during priming abrogates the block of inhibition. Finally, if neurons are exposed to MAG/myelin and neurotrophins simultaneously, but with the Gi protein inhibitor, inhibition is blocked. We suggest that priming neurons with particular neurotrophins elevates cAMP and activates PKA, which blocks subsequent inhibition of regeneration and that priming is required because MAG/myelin activates a Gi protein, which blocks increases in cAMP. This is important for encouraging axons to regrow in vivo.  (+info)

Mechanisms for generating the autonomous cAMP-dependent protein kinase required for long-term facilitation in Aplysia. (7/7238)

The formation of a persistently active cAMP-dependent protein kinase (PKA) is critical for establishing long-term synaptic facilitation (LTF) in Aplysia. The injection of bovine catalytic (C) subunits into sensory neurons is sufficient to produce protein synthesis-dependent LTF. Early in the LTF induced by serotonin (5-HT), an autonomous PKA is generated through the ubiquitin-proteasome-mediated proteolysis of regulatory (R) subunits. The degradation of R occurs during an early time window and appears to be a key function of proteasomes in LTF. Lactacystin, a specific proteasome inhibitor, blocks the facilitation induced by 5-HT, and this block is rescued by injecting C subunits. R is degraded through an allosteric mechanism requiring an elevation of cAMP coincident with the induction of a ubiquitin carboxy-terminal hydrolase.  (+info)

Marker genes of decidualization: activation of the decidual prolactin gene. (8/7238)

Decidualization of human endometrial stromal (ES) cells in vitro is induced by cAMP analogues and ligands that elevate cellular cAMP levels in a manner resembling the gonadotrophins, prostaglandin E2 and relaxin (RLX). This differentiation process is marked by the onset of decidual prolactin (PRL) production in the late luteal phase of the cycle. Using transfection assays and a primary ES cell culture system, we have demonstrated that decidual PRL gene transcription is driven by an alternative upstream promoter (dPRL), approximately 6 kb upstream of the pituitary transcription start site. In primary cell cultures, RLX not only acutely but also permanently elevated cellular cAMP levels and induced PRL secretion after 6 days. Northern and Western blot analyses revealed all regulatory subunit isoforms (RIalpha, RIbeta, RIIalpha, RIIbeta) and catalytic subunits Calpha and Cbeta of protein kinase A (PKA) in ES cells. Transcript levels of PKA subunit isoforms are not altered during decidualization, but in decidualized ES cells exposed to elevated cellular cAMP levels by stimulation with RLX for >6 days, RIalpha protein levels were significantly reduced, whereas levels of all other forms remained unchanged. Reducing the availability of R subunits changed the R:C subunit ratio in favour of C and increased kinase activity. In transient transfections of undifferentiated ES cells, the dPRL promoter was activated by 8-Br-cAMP and by C subunit (Cbeta) of PKA. This induction, and the differentiation-dependent activity of the dPRL promoter in transfected decidualized cells, was effectively abolished by the co-expression of protein kinase inhibitor (PKI). A fragment of 332 bp of 5'-flanking region of the dPRL transcription start site was sufficient to mediate full inducibility by cAMP. cAMP activation of the dPRL promoter in ES cells was biphasic as an initial weak induction within 12 hours was followed by a subsequent, much more intense induction after 12 hours. The secondary induction was not seen with a control construct driven by a consensus cAMP response element (CRE) linked to a minimal promoter. The early response of the dPRL promoter depended upon a non-palindromic CRE at position -12 and mutation of this sequence led to omission of the early, but not of the delayed, induction. The major activation of the dPRL promoter depended upon a different region between position -332 and -270 since its deletion significantly reduced inducibility by cAMP. Its action was probably indirect as its kinetics differed from classic CRE-mediated responses, and it was specific to ES cells.  (+info)

Cardiac myocyte ryanodine receptors (sarcoplasmic reticulum [SR] Ca release channel; cardiac ryanodine receptor [RyR2]) are localized in the junctional SR, in close proximity to L-type Ca channels (LTCCs) embedded in the membranes of the transverse (T)-tubules. This signaling microdomain has been termed the couplon,1 because it is here that excitation-contraction coupling takes place. As the heart fills with blood during diastole, RyR2 is stabilized in a closed state, allowing Ca uptake by the SR Ca ATPase to pump Ca from the cytoplasm into the SR, providing the primary source of Ca to activate the contractile apparatus during the next heart beat (systole). During systole, the cardiac action potential depolarizes the T-tubules and causes the opening of LTCCs. Ca influx through LTCCs elevates the [Ca] within the cytoplasmic space between the T-tubular and SR membrane, which promotes Ca binding to neighboring RyR2s, inducing some to open. Ca then moves out of the SR lumen into the subsarcolemmal, ...
Adenosine suppresses protein kinase A- and C-induced enhancement of glutamate release in the hippocampus.: Cultured hippocampal neurons from neonatal rats were
ChemicalBook provide Chemical industry users with GUANOSINE-3,5-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT Boiling point Melting point,GUANOSINE-3,5-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT Density MSDS Formula Use,If You also need to GUANOSINE-3,5-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT Other information,welcome to contact us.
ChemicalBook provide Chemical industry users with 8-CHLOROADENOSINE-3,5-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT Boiling point Melting point,8-CHLOROADENOSINE-3,5-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT Density MSDS Formula Use,If You also need to 8-CHLOROADENOSINE-3,5-CYCLIC MONOPHOSPHOROTHIOATE, RP-ISOMER SODIUM SALT Other information,welcome to contact us.
The data described above demonstrate a local PDE feedback regulation in cardiac myocytes whereby β1AR signals activate PDE4B3 via PKA-mediated phosphorylation. PKA-activated PDE4B, in turn, reduces the local steady-state cAMP concentration in a confined subsarcolemmal domain. The functional consequences of interrupting this feedback include increased PKA-mediated phosphorylation of Cav1.2 and RyR2, which is necessary for fine-tuning of ECC, a finding consistent with the cardiac phenotype of PDE4BKO mice (Leroy et al., 2011). Functional data on intracellular Ca2+ levels and contraction rate confirm the altered cAMP/PKA signaling in PDE4BKO myocytes. These conclusions are based on measurements of βAR-induced cAMP accumulation at the plasma membrane of PDE4BKO myocytes, the effects of acute PDE4B-selective inhibition in wild-type myocytes, the β1AR-dependent activation of PDE4B and the altered PKA-mediated phosphorylation of some, but not all substrates involved in Ca2+ homeostasis and ...
Voltage-gated sodium channels, which initiate action potentials in mammalian brain neurons, are modulated functionally by cAMP-dependent protein kinase A (PKA), resulting in reduced sodium current amplitude. Comparing brain and muscle sodium channels, we show that only the brain channel is modulated by PKA. The brain sodium channel I-II linker is both necessary and sufficient for PKA modulation, as shown by exchanging the I-II linker regions of the two channels. PKA consensus sites in the brain channel I-II linker were eliminated by deletion and site-specific mutagenesis. The mutant channels demonstrated decreased levels of phosphorylation when metabolically labeled in oocytes with [gamma-32P]-ATP, and they did not respond with a reduction in current magnitude after PKA induction. Modulation of the brain channel by PKA phosphorylation was mimicked by adding fixed negative charges at the PKA consensus sites, suggesting that the decrease in current was a direct result of the negative charge at one ...
We have previously shown that the protein kinase inhibitor β (PKIβ) form of the cAMP-dependent protein kinase inhibitor exists in multiple isoforms, some of which are specific inhibitors of the cAMP-dependent protein kinase, whereas others also inhibit the cGMP-dependent enzyme [Kumar, Van Patten and Walsh (1997), J. Biol. Chem. 272, 20011-20020]. We have now demonstrated that the switch from a cAMP-dependent protein kinase (PKA)-specific inhibitor to one with dual specificity arises as a consequence of alternate gene splicing. We have confirmed using bacterially produced pure protein that a single inhibitor species has dual specificity for both PKA and cGMP-dependent protein kinase (PKG), inhibiting each with very high and closely similar inhibitory potencies. The gene splicing converted a protein with 70 amino acids into one of 109 amino acids, and did not change the inhibitory potency to PKA, but changed it from a protein that had no detectable PKG inhibitory activity to one that now ...
cAMP-dependent protein kinase complex, cytoplasm, nucleus, cAMP-dependent protein kinase activity, mitochondrion organization, protein kinase A signaling, protein phosphorylation, Ras protein signal transduction
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis ...
Protein Kinase A-Dependent Derepression of the Human Prodynorphin Gene via Differential Binding to an Intragenic Silencer Element: Induction of the prodynorphin
Protein kinases are able to recognize their appropriate targets in a complex milieu of cellular protein. This process must be carried out with high fidelity to ensure proper signal transduction in eukaryotic cells (Hunter 2000). In this study, we attempted to obtain insight into this recognition by examining PKA variants that exhibit a stable association with substrates. This binding provided a facile assay that allowed us to identify domains in both enzyme and substrates that were important for PKA phosphorylation. The substrate domains identified were physically removed from the sites of phosphorylation and were required for efficient recognition by PKA both in vivo and in vitro. To the best of our knowledge, these studies are the first to show that such distal sequence elements in substrates are required for phosphorylation by PKA. These observations may help explain why only a fraction of proteins that contain a PKA consensus site are phosphorylated by this enzyme in vivo (Budovskayaet al. ...
Neuromodulators have profound effects on behavior, but the dynamics of their intracellular effectors has remained unclear. Most neuromodulators exert their function via G-protein-coupled receptors (GPCRs). One major challenge for understanding neuromodulator action is the lack of dynamic readouts of the biochemical signals produced by GPCR activation. The adenylate cyclase/cyclic AMP/protein kinase A (PKA) module is a central component of such biochemical signaling. This module is regulated by several behaviorally important neuromodulator receptors. Furthermore, PKA activity is necessary for the induction of many forms of synaptic plasticity as well as for the formation of long-term memory. In order to monitor PKA activity in brain tissue, we have developed a 2-photon fluorescence lifetime imaging microscopy (2pFLIM) compatible PKA sensor termed FLIM-AKAR, which is based on the ratiometric FRET sensor AKAR3. FLIM-AKAR shows a large dynamic range and little pH sensitivity. In addition, it is a ...
The first indication that PKA-mediated phosphorylation of the β2AR might do more than simply desensitize the receptor by inhibiting its coupling to Gs was provided by Okamoto et al. (1991). They demonstrated that a short peptide derived from the third cytoplasmic loop of the β2AR effectively activated purified Gs but only very weakly activated Gi in vitro. This peptide contains one of the two consensus PKA phosphorylation sites found in the receptor. When the peptide was phosphorylated by PKA, its ability to activate Gs was dramatically reduced, whereas its ability to activate Gi was reciprocally increased.. These results are essentially identical to findings in recent in vitro reconstitution studies with the intact recombinant human β2AR and recombinant Gsand Gi (Zamah et al., 2002). Reconstituted native β2AR mediates robust activation of Gs but not Gi. When the receptor is phosphorylated in vitro by PKA, Gscoupling is reduced, but Gi activation is markedly enhanced. The results are ...
The major objective of the present study was to further our understanding of the molecular mechanisms by which perturbations of the cAMP pathway regulate PKA subunit expression in neural cells. Indeed, the cAMP-induced down-regulation of PKA subunits described here contrasts with the findings reported for non-neural systems. In Sertoli cells, an extensively studied model system for PKA regulation (reviewed in Skålhegg and Taskén, 1997), activation of the cAMP pathway elevates PKA subunit protein levels along with a 2- to 4-fold increase in RIα, RIIα and Cα mRNA and a 50-fold increase in RIIβ mRNA. This up-regulation of mRNA levels involved both increased transcription and increased mRNA stability. In mouse epithelial cells (Lange-Carter and Malkinson, 1991), elevated levels of cAMP resulted in a similar increase in RIIβ mRNA but a decrease in mRNA for RIα and no change in that for RIIα. Earlier studies have suggested cAMP stimulated proteolytic degradation of C, but not R, subunits in ...
42 of the Chromas software package (Conor McCarthy, Southport, Australia). For all analyses, data obtained PLX3397 purchase with the forward and reverse primers were combined and aligned to the consensus sequence obtained from the BLAST GenBank database http://​www.​ncbi.​nlm.​nih.​gov/​nuccore/​166706780?​report=​genbank. Figure 1 Sequencing of the KRAS gene in DNA isolated from NSCLC tissues. (A) Wild type-(12Gly-GGT, 13Gly-GGC), (B) Mutant- (12Asp-GAT). Pyrosequencing In the pyrosequencing method for DNA sequence analysis [16, 17], inorganic phosphate released in the course of nucleotide incorporation serves as the initial substrate in a sequence of four. successive enzymatic reactions. This result in the emission of light, which functions as a signal that is proportional to the number of nucleotides incorporated. In this project, the PyroMark K-ras assay test (Biotage, Uppsala, Sweden) was used for primary amplification P005091 cost and pyrosequencing of both the 12th and ...
Dive into the research topics of Characterization and comparison of membrane-associated and cytosolic cAMP-dependent protein kinases. Physicochemical and immunological studies on bovine cerebral cortex protein kinases.. Together they form a unique fingerprint. ...
Intracellular Ca2+ cycling and associated signaling pathway in cardiomyocytes. On a beat-by-beat basis, a calcium transient is evoked by the initial influx of a small amount of Ca2+ through the LTCC and the subsequent large-scale Ca2+ release from the SR through the RyR. During diastole, cytosolic Ca2+ is taken up into the SR by the PLN-regulated SERCA2a pump. β receptor-mediated PKA stimulation regulates this Ca2+ cycling by phosphorylating LTCC, RyR, and PLN. In normal hearts, sympathetic stimulation activates β1-adrenergic receptor, which in turn stimulates the production of cAMP by adenylyl cyclase and thereby activates PKA. PKA phosphorylates PLN and RyR, both of which contribute to an increased intracellular Ca2+ transient and enhanced cellular contractility (pink zone signal). PP1 and PP2A regulate the dephosphorylation process of these Ca2+ regulatory proteins (RyR, PLN, LTCC) (blue zone signaling). Activation of the Gαq-coupled receptors (angiotensin II receptor, endothelin 1 ...
Guanosine 3ʹ,5ʹ-cyclic Monophosphorothioate, 8-Bromo-, Rp-Isomer, Sodium Salt - Calbiochem - Find MSDS or SDS, a COA, data sheets and more information.
Second, the biological response to a second messenger can depend on the scaffolding complex that binds enzymes that synthesize or degrade the second messenger, and effectors (Figure 1B). There are numerous examples of this mode of operation in cells. A-kinase anchoring proteins (AKAPs) are known to localize cAMP-dependent protein kinase (PKA) to different subcellular compartments in the cell, thereby ensuring phosphorylation of PKA targets in the correct cellular vicinity.16 Some AKAPs have also been found to associate with cAMP-hydrolyzing PDE isoforms, thereby providing a mechanism for fine-tuning local cAMP levels and downstream effects of PKA.17 The PDE4D splice variant PDE4D3 binds to muscle-selective AKAP (mAKAP), and this association results in low cAMP levels and prevents PKA activation under basal conditions. Following stimulation of cAMP synthesis, however, PKA is activated and phosphorylates mAKAP. These phosphorylation events result in a more efficient PDE4D3 action, and provides a ...
TY - JOUR. T1 - cAMP-dependent protein kinase. T2 - role in normal and malignant growth. AU - Cho-Chung, Yoon S.. AU - Pepe, Stefano. AU - Clair, Timothy. AU - Budillon, AlfreD O.. AU - Nesterova, Maria. PY - 1995. Y1 - 1995. UR - http://www.scopus.com/inward/record.url?scp=0029565543&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0029565543&partnerID=8YFLogxK. U2 - 10.1016/1040-8428(94)00166-9. DO - 10.1016/1040-8428(94)00166-9. M3 - Article. C2 - 8822496. AN - SCOPUS:0029565543. VL - 21. SP - 33. EP - 61. JO - Critical Reviews in Oncology/Hematology. JF - Critical Reviews in Oncology/Hematology. SN - 1040-8428. IS - 1-3. ER - ...
In our work we develop and analyze an ordinary differential equation. model that describes the cyclic adenosine monophosphate (cAMP) --Protein Kinase A (PKA) pathway in budding yeast. In particular our. model describes the effect of glucose stimulation on the concentration of cAMP in the short term,. and the effect of stress in the long term. We develop this model in. order to understand two specific experimental results, reported by. Ma et al. (1999) and Garmendia-Torres et al. (2007). In order to describe the. surprising results published by Ma et al. (1999) we make a key assumption. that three enzymes within the cAMP-PKA network compete with one. another for activation by PKA. This assumption sets our model apart. from previous models of the cAMP-PKA network.. Our model focuses on two forms of negative feedback that. drive oscillations in the concentration of cAMP. Under high or low. stress conditions (for example, following glucose stimulation) our model reduces to a single ...
The cyclic AMP-dependent protein kinase, PKA, has been proposed to regulate many aspects of β-cell biology, including insulin secretion, insulin synthesis, β-cell survival and β-cell proliferation. We have established β-caPKA mice, a model of tamoxifen-inducible PKA activity that is targeted specifically to the islet β-cells. We have shown that enhanced insulin secretion in β-caPKA mice tightens glucose control and that this not dependent upon increased β-cell mass. Enhanced insulin secretion and improved glucose control was maintained to 52 weeks of age but β-cell mass at 52 weeks of age did not differ between β-caPKA mice and littermate controls, indicating again that activation of PKA in the islet β-cells does not promote β-cell proliferation. To determine whether PKA activity is protective for β-cells, PKA activity was induced in β-caPKA mice, then streptozotocin was administered to induced β-cell destruction. Increasing PKA activity in β-cells protected them from ...
|P>PKA (Protein Kinase-A) is an enzyme that regulates processes as diverse as growth, development, memory, and metabolism. In its inactivated state, PKA exists as a tetrameric complex of two Catalytic subunits (PKA-C) and a Regulatory (PKA-R) subunit dimer. To date, [...]
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Lapatinib was provided alone for six weeks,just before sufferers had been taken care of with trastuzumab and chemotherapy for 12 weeks prior to surgical treatment.In the course of the primary six weeks of lapatinib therapy,tumor volumes total have been decreased.Matched pre- and post-lapatinib therapy biopsies with ample tumor materials were accessible from eight patients for RNA isolation and microarray Ecdysone hybridization to Affymetrix GeneChips.We in contrast the intensity of expression for probesets corresponding to Src,Yes,Fyn,Lyn,Lck,and Hck ahead of and after lapatinib.We identified statistically important increases in expression of approximately 2-fold for 7 probesets corresponding to Lyn,Lck,and Fyn.Unfortunately,the Y416 pSrc antibody in our hands was inadequate for trustworthy quantitation of immunohistochemistry in these samples.Inhibition of SFKs inhibits development and PI3K-Akt in lapatinib-resistant cells To determine if SFK inhibition in drug-resistant cells would restore ...
1BKX: A binary complex of the catalytic subunit of cAMP-dependent protein kinase and adenosine further defines conformational flexibility.
In the hippocampus, the cAMP dependent protein kinase (PKA) plays critical roles in neurotransmission, cell excitability, and synaptic plasticity.All of these p...
cAMP-dependent protein kinase (PKA), ubiquitous in mammalian cells, regulates a plethora of cell processes including development, differentiation, memory, and m...
Sinerik Ayrapetyan-The Dysfunction of Camp-Dependent Na+/Ca2+ Exchange in Reverse Mode as a Primary Mechanism for Age-Dependent Cardio-Muscle Failure
TY - JOUR. T1 - Protein kinase A-anchoring inhibitor peptides arrest mammalian sperm motility. AU - Vijayaraghavan, Srinivasan. AU - Goueli, Said A.. AU - Davey, Michael. AU - Carr, Daniel. PY - 1997/2/21. Y1 - 1997/2/21. N2 - Cyclic AMP-dependent protein kinase (PKA) is anchored at specific subcellular sites through the interaction of the regulatory subunit (R) with protein kinase A-anchoring proteins (AKAPs) via an amphipathic helix binding motif. Synthetic peptides containing this amphipathic helix domain competitively disrupt PKA binding to AKAPs and cause a loss of PKA modulation of cellular responses. In this report we use S-Ht31, a cell-permeant anchoring inhibitor peptide, to study the role of PKA anchoring in sperm. Our analysis of three species of mammalian sperm detected three isoforms of PKA (RIIα, RIIβ, and RIβ) and one 110-kDa AKAP. The addition of S-Ht31 to bovine caudal epididymal sperm inhibits motility in a time- and concentration-dependent manner. A control peptide, ...
Looking for online definition of cAMP-dependent protein kinase in the Medical Dictionary? cAMP-dependent protein kinase explanation free. What is cAMP-dependent protein kinase? Meaning of cAMP-dependent protein kinase medical term. What does cAMP-dependent protein kinase mean?
In the present study we demonstrated that IL-13, a Th2 cell-derived cytokine, is a potent arginase activator, and its induction of arginase contributes significantly to the suppression of NO production in LPS-activated macrophages. The increase in arginase activity is a result of de novo synthesis of arginase I mRNA and protein. Studies on the signaling molecules involved in arginase activation show that a surge in intracellular cAMP and the subsequent activation of PKA are obligatory for arginase induction. In addition, tyrosine kinases and p38 MAPK play a role in IL-13-induced arginase activation. To provide a perspective on our observations and conclusions, the results from the present study are discussed below in reference to previous findings regarding the signaling pathways involved in arginase activation and NO regulation by arginase.. In the present study despite the basal level of arginase I gene expression being detected in resting macrophages, arginase protein expression and enzyme ...
Lawler OA, Miggin SM, Kinsella BT (2001). Protein kinase A-mediated phosphorylation of serine 357 of the mouse prostacyclin receptor regulates its coupling to G(s)-, to G(i)-, and to G(q)-coupled effector signaling.. J. Biol. Chem. 276 (36): 33596-607. PMID 11443126. doi:10.1074/jbc.M104434200. ...
TY - JOUR. T1 - Characterization of the bovine lens plasma membrane substrates for cAMP‐dependent protein kinase. AU - LOUIS, Charles F.. AU - JOHNSON, Ross. AU - JOHNSON, Keith. AU - TURNQUIST, Janet. PY - 1985/7. Y1 - 1985/7. N2 - cAMP‐dependent protein kinase, derived from either calf lens or bovine heart, promotes the phosphorylation of three lens plasma membrane proteins of molecular mass 28 kDa, 26 kDa and 18 kDa. Correlation of the maximal level of phosphorylation of these components with the Coomassie blue staining intensity of fractionated lens membranes suggests that the phosphorylation of the 28 kDa and 18 kDa components may be approximately stoichiometric. The protein kinase substrates could be dephosphorylated by a cardiac sarcoplasmic‐reticulum‐bound protein phosphatase activity. The 26 kDa component comigrated with MP26, the major lens membrane component that has been localized to the lens fiber cell junction. Treatment of phosphorylated lens membranes with chymotrypsin ...
cansSAR 3D Structure of 5N3F | CAMP-DEPENDENT PROTEIN KINASE A FROM CRICETULUS GRISEUS IN COMPLEX WITH FRAGMENT LIKE MOLECULE N-[3-(AMINOMETHYL)PHENYL]ACETAMIDE | 5N3F_A | cAMP-dependent protein kinase catalytic subunit alpha - Also known as KAPCA_CRIGR, PRKACA. Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B
The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015 ...
In the plant, filamentous growth is required for pathogenicity of the corn smut pathogen Ustilago maydis. Earlier, we identified a role for the cAMP signal transduction pathway in the switch between budding and filamentous growth for this fungus. A gene designated ubc1 (for Ustilago bypass of cyclase) was found to be required for filamentous growth and to encode the regulatory subunit of a cAMP-dependent protein kinase (PKA). Here, we show that ubc1 is important for the virulence of the pathogen. Specifically, ubc1 mutants are able to colonize maize plants and, like the wild-type pathogen, cause localized symptoms in association with the presence of hyphae. However, in contrast to plants infected with wild-type cells that often developed galls from initially chlorotic tissue, plants infected with the ubc1 mutant did not produce galls. These data suggest that PKA regulation is critical for the transition from saprophytic to pathogenic growth and from vegetative to reproductive development. Plate ...
The occurrence of endogenous substrate proteins for Ca2+-dependent protein kinase, augmented by either phospholipid or calmodulin, and for cyclic AMP-dependent protein kinase was examined in homogenates and subcellular fractions of mouse pancreatic islets. Islet protein phosphorylation was enhanced by Ca2+-calmodulin; the major endogenous substrates in the homogenate were two proteins of Mr 53000 and 100000. The Mr-100000 phosphoprotein was localized to a 27000g-supernatant fraction, whereas the Mr-53000 phosphoprotein was present in a 27000g particulate fraction of mouse islets. In the presence of Ca2+, phosphatidylserine stimulated phosphorylation of 15 proteins, of Mr 17000-190000, in a 27000g-supernatant fraction. No effects of Ca2+ plus phosphatidylserine were observed in a 27000g particulate fraction of mouse islets. Examination of cyclic AMP-dependent protein phosphorylation revealed five substrate proteins, of Mr 23000-72000, present in the 27000g supernatant of mouse islets. No common ...
TY - JOUR. T1 - Cloning, characterization, and expression of the gene for the catalytic subunit of cAMP-dependent protein kinase in Caenorhabditis elegans. T2 - Identification of highly conserved and unique isoforms generated by alternative splicing. AU - Gross, Robert E.. AU - Bagchi, Srilata. AU - Lu, Xiangyi. AU - Rubin, Charles S.. PY - 1990. Y1 - 1990. N2 - The nematode Caenorhabditis elegans expresses substantial amounts of several forms (Mr values = 39,000-41,000) of the catalytic subunit (C) of cAMP-dependent protein kinase. Approximately 65% of the total cAMP-dependent phosphotransferase activity is recovered in particulate fractions of homogenates prepared from asynchronous populations of C. elegans. The C subunit is expressed at a low level in cytosolic and particulate compartments during embryogenesis. As the nematodes progress from late embryonic stages to the newly hatched, first larval (L1) stage, C subunit content increases 15-fold. High levels of C subunits are observed in ...
cAMP-dependent protein kinase inhibitor beta is a protein that in humans is encoded by the PKIB gene. The protein encoded by this gene is a member of the cAMP-dependent protein kinase inhibitor family. Studies of a similar protein in rat suggest that this protein may interact with the catalytic subunit of cAMP-dependent protein kinase and act as a competitive inhibitor. At least three alternatively spliced transcript variants encoding the same protein have been reported. GRCh38: Ensembl release 89: ENSG00000135549 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000019876 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Zheng L, Yu L, Tu Q, Zhang M, He H, Chen W, Gao J, Yu J, Wu Q, Zhao S (Jan 2001). Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, including PKIA. Biochem J. 349 (Pt 2): 403-7. doi:10.1042/0264-6021:3490403. PMC 1221161 . PMID 10880337. Entrez Gene: ...
TY - JOUR. T1 - Differential regulation of synaptic GABA(A) receptors by cAMP-dependent protein kinase in mouse cerebellar and olfactory bulb neurones. AU - Nusser, Zoltan. AU - Sieghart, Werner. AU - Mody, Istvan. PY - 1999/12/1. Y1 - 1999/12/1. N2 - 1. It has been demonstrated that the regulation of recombinant GABA(A) receptors by phosphorylation depends on the subunit composition. Here we studied the regulation of synaptic GABA(A) receptor function by cAMP-dependent protein kinase (PKA) in neurones expressing distinct receptor subtypes. 2. Light microscopic immunocytochemistry revealed that granule cells of the olfactory bulb express only the β3 as the β subunit variant, whereas cerebellar stellate and basket cells express only the β2 as the β subunit. 3. In cerebellar interneurones, intracellular application of 20 μM microcystin, a protein phosphatase 1/2A inhibitor, prolonged (63 ± 14%; mean ± S.E.M.) the decay time course of miniature IPSCs (mIPSCs) without significantly affecting ...
A-kinase anchor protein 10, mitochondrial (AKAP-10) (Dual specificity A kinase-anchoring protein 2) (D-AKAP-2) (Protein kinase A-anchoring protein 10) (PRKA10), ...
We identified pka-C1 as a candidate for the gene mutated in Cos12, Cos13, Cos18, and Cos19. We detected sequence changes at the DNA level at the pka-C1 locus, and these changes translated to substitutions in the coding sequence. These point mutations could influence PKA activity in several possible ways. First, they could render PKA-C1 catalytically inactive and give dominant phenotypes due to haplo-insufficiency. Alternatively, the point mutations could destabilize the encoded protein to such a degree that the mutant protein would be degraded and thus function as a protein null. This scenario would also result in dominant phenotypes due to haplo-insufficiency. These haplo-insufficiency explanations are unlikely to be correct. pka-C1 is a recessive negative regulator of Hh signaling and heterozygosity for pka-C1 null alleles or for deficiencies that delete pka-C1 does not result in any obvious phenotype. Second, the mutations could render PKA-C1 constitutively active. The unregulated activity ...
Moen, Line Victoria; Ramberg, Håkon Andre; Zhao, Sen; Grytli, Helene Hartvedt; Sveen, Anita; Berge, Viktor; Skotheim, Rolf I.; Tasken, Kristin Austlid & Skålhegg, Bjørn Steen (2017). Observed correlation between the expression levels of catalytic subunit, C?2, of cyclic adenosine monophosphate-dependent protein kinase and prostate cancer aggressiveness. Urologic Oncology. ISSN 1078-1439. 35(3), s 111.e1- 111.e8 . doi: 10.1016/j.urolonc.2016.10.002 Vis sammendrag Background As an intracellular human pathogen, Mycobacterium tuberculosis (Mtb) is facing multiple stressful stimuli inside the macrophage and the granuloma. Understanding Mtb responses to stress is essential to identify new virulence factors and pathways that play a role in the survival of the tubercle bacillus. The main goal of this study was to map the regulatory networks of differentially expressed (DE) transcripts in Mtb upon various forms of genotoxic stress. We exposed Mtb cells to oxidative (H2O2 or paraquat), nitrosative ...
Cyclic AMP is a ubiquitous intracellular second messenger involved in the regulation of a wide variety of cellular processes, a majority of which act through the cAMP - protein kinase A (PKA) signalling pathway and involve PKA phosphorylation of specific substrates. PKA phosphorylation events are typically spatially restricted and temporally well controlled. A-kinase anchoring proteins (AKAPs) directly bind PKA and recruit it to specific subcellular loci targeting the kinase activity towards particular substrates, and thereby provide discrete spatiotemporal control of downstream phosphorylation events. AKAPs also scaffold other signalling molecules into multi-protein complexes that function as crossroads between different signalling pathways. Targeting AKAP coordinated protein complexes with high-affinity peptidomimetics or small molecules to tease apart distinct protein-protein interactions (PPIs) therefore offer important means to disrupt binding of specific components of the complex to better
Background The cAMP-dependent protein kinase (PKA) signaling transduction pathway has been shown to play an important role in the modulation of several ethanol (EtOH)-induced behavioral actions. In vivo, short-term exposure to EtOH up-regulates the cAMP-signaling cascade. Interestingly, different Ca2+-dependent cAMP-PKA cascade mediators play a critical role in the neurobehavioral response to EtOH, being of special relevance to the Ca2+-dependent adenylyl cyclases 1 and 8. We hypothesize an intracellular PKA activation elicited by EtOH administration, which may be regulated by a Ca2+-dependent mechanism as an early cellular response. Thus, the present work aims to explore the role of Ca2+ (internal and external) on the EtOH-activated PKA cascade. Methods Swiss male mice received an intraperitoneal injection of EtOH (0 or 4 g/kg), and brains were dissected following a temporal pattern (7, 15, 30, 45, 90, or 120 minutes). Either the enzymatic PKA activity or its fingerprint was analyzed on ...
Similarly to AA-dependent calcium entry, NO-activated calcium signals triggered by direct application of NO donors are significantly affected on pretreatment with PKI (Fig. 5B and C).. We do not know the identity of AA- and NO-activated channel(s) in B-TECs, although some candidates can be considered. TRPV1 and TRPV4 (both expressed in B-TEC) 5 are regulated by fatty acids, including AA, and their metabolites. They are also modulated by NO through S-nitrosylation and sensitized by PKA phosphorylation (37-40). TRPC3 and TRPC6 are activated by fatty acids and substrate for nitrosylation as well, and the cAMP/PKA pathway could enhance their insertion into the plasma membrane (34). Finally, in m3-HEK cell line, the so-called arachidonate-regulated channels require PKA phosphorylation and are composed of Orai1 and Orai3 proteins (35, 41).. B-TECs could express two (or more than two) types of calcium-permeable, PKA-dependent ion channels: one activated by AA and another by NO. Such hypothesis is in ...
ウサギ・ポリクローナル抗体 ab96186 交差種: Ms,Hu 適用: WB,ICC/IF…cAMP Protein Kinase Catalytic subunit抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody…
In this issue of Acta Physiologica, Benz et al. study the role of one important protein in the cyclic AMP signaling pathway, the A-kinase anchoring (AKAP)12. The downstream effects stemming from cAMP release are tightly controlled and activate a profusion of signaling pathways. However, many of these different processes function with largely the same major constituent proteins, including adenylate cyclases, kinases, phosphatases, and phosphodiesterases. cAMP-dependent protein kinase (PKA), which is the main intracellular target for cAMP, is widely found in these signaling assemblies, and is present at high concentrations in many tissues, playing varied roles in the regulation of molecular processes. Unexpectedly, despite its ubiquity there are only four isoforms of PKA regulatory subunit with which to impart functional and locational specificity ...
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Doherty, P J.; Tsao, J; Schimmer, B P.; Mumby, M C.; and Beavo, J A., Alteration of the regulatory subunit of type 1 camp-dependent protein kinase in mutant y1 adrenal cells resistant to 8-bromoadenosine 3:5-monophosphate. (1982). Subject Strain Bibliography 1982. 2372 ...
Amp protein powder review 2020, that happened after 12 weeks! Results amaze, side effects could be dangerous for some users | Important tips & pictures
Both types of reciprocal antagonistic A2A-D2 receptor interactions coexist in the same cells. In fact, under normal conditions, there is a strong tonic activation of D2 receptors that blocks the ability of A2A receptors to signal through the cAMP-PKA pathway. Conversely, the antagonistic A2A-D2 receptor interaction determines the ability of A2A receptors to control the inhibitory role of D2 receptors in neuronal excitability and neurotransmitter release (Ferré et al., 2008).. In line with our previous studies (Calabresi et al., 1993; Picconi et al., 2004; Tozzi et al., 2007), we found that the application of D2 receptor agonists alone did not affect glutamate-mediated synaptic potentials/currents in striatal slices under physiological conditions. Conversely, simultaneous A2A receptor antagonism and D2 receptor activation resulted in a reduction of excitatory glutamatergic transmission. In our model, electrical stimulation of the slice mainly activates glutamatergic projections to the striatum. ...
Subcellular fractionation revealed that pp97, pp96 and pp64 are head protein whereas pp90 and pp55 are tail proteins. Advanced proteomic analysis (GeLC-MS) identified 37 proteins, including AKAP4, AKAP3, CALI, HSPAlL and HSP70 as candidates for the dephosphorylated proteins. AKAP4 was excluded because it was localised to the tail. Two AKAP3 antibodies showed non-specific binding and a better quality antibody will be needed for further investigations. CALI was excluded because it was localised to the tail fraction. HSP70/72 and HSPA1L were strong candidates for pp64. However, immunoprecipitation of dephosphorylated proteins using phospho (S/T) PKA substrate Ab and HSPA1L Ab was unsuccessful and further work is now required to address this.. ...
Zhao X, Nedvetsky P, Stanchi F, Vion AC, Popp O, Zühlke K, Dittmar G, Klussmann E, Gerhardt H. 2019. Endothelial PKA activity regulates angiogenesis by limiting autophagy through phosphorylation of ATG16L1. eLife 8:e46380. doi: 10.7554/eLife.46380.. Published 03, October 2019. We have discovered a panel duplication in Figure 4 supplement 1C and D. It appears that during reorganisation of this Figure supplement in the course of revisions, panel 1C was duplicated as 1D. The correct panel was present in the original version of the figure, showing the quantification of ESM1 positive area in 1D. We further discovered a small typesetting error in the method section which read ml/min instead of µl/min. We would like to correct these errors as follows. The corrections do not affect data or conclusions.. The corrected text (change is underlined):. Five microliters of the sample was loaded on a nano-LC column (0.074 × 250 mm, 3 mm Reprosil C18; Dr. Maisch) and separated on a 155 min gradient (4%-76% ...
Full-length recombinant human PRKX was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PRKX is a serine/threonine protein kinase that is closely related to the catalytic subunit of the cAMP-dependent protein kinase.
Full-length recombinant human PRKX was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PRKX is a serine/threonine protein kinase that is closely related to the catalytic subunit of the cAMP-dependent protein kinase.
Bacterial expression plasmid for GST tagged with a PKA phosphorylation site. Described in Ron D, Dressler H. pGSTag: a versatile bacterial expression plasmid for enzymatic labeling of recombinant proteins. Biotechniques. 1992 Dec;13(6):866-9. Erratum in: Biotechniques 1993 Feb;14(2):221. Plasmid sequence in EMBL format is available for downloading here. ...
Complete information for AKAP5 gene (Protein Coding), A-Kinase Anchoring Protein 5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
20E transmits signals through the ErGPCR-cAMP-PKA-CREB signaling pathway prior to the initiation of the gene expression through the EcR/UPS pathway
Camp Nursing, Camp nursing has its own unique twists: you are charged with the health care of many unknowns and this is where you can come to for answers. How do you... - pg. 7
ค่ายนี้เป็นค่ายระดับมัธยมศึกษา สำหรับเยาวชนที่สนใจการศึกษาต่อในสาขาชีวเคมี. จัดขึ้นเป็นครั้งแรกเมื่อเดือนมกราคม 2558 โดยนิสิตภาควิชาชีวเคมี คณะวิทยาศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย. ชมมิวสิควิดิโอ What is the pathway? ซึ่งจัดทำขึ้นโดยนิสิตภาควิชาชีวเคมี คณะวิทยาศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย. ...
Nguyen, P. V., & Woo, N. H. (2003). Regulation of hippocampal synaptic plasticity by cyclic AMP-dependent protein kinases. ... For example, the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) pathways appear to participate in neuronal ... cyclic AMP responsive element-binding protein) in this process. Certain synapses on recruited neurons are more likely to ... The dendritic branch is the preferred integrative unit for protein synthesis-dependent LTP. Neuron 69, 132-146 (2011). Harvey, ...
Pan, X, Heitman, J (July 1999). "Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces ... Pan, X, Heitman, J (June 2002). "Protein kinase A operates a molecular switch that governs yeast pseudohyphal differentiation ... Lorenz, MC, Pan, X, Harashima, T, Cardenas, ME, Xue, Y, Hirsch, JP, Heitman, J (February 2000). "The G protein-coupled receptor ... Lorenz, MC, Heitman, J (December 1997). "Yeast pseudohyphal growth is regulated by GPA2, a G protein alpha homolog". EMBO ...
"Phosphorylation and activation of brain aromatic L-amino acid decarboxylase by cyclic AMP-dependent protein kinase". Journal of ... AADC has several conserved protein kinase A (PKA) and protein kinase G recognition sites, with residues S220, S336, S359, T320 ... It consists of 15 exons encoding a protein of 480 amino acids. Single nucleotide polymorphisms and other gene variations have ... The short term activation is thought to proceed through kinase activation and subsequent phosphorylation of AADC, while the ...
They are Gs/a coupled and can stimulate neurons by indirectly activating cyclic AMP-dependent protein kinase. The DRD1 gene ... Dopamine receptor D1, also known as DRD1, is a protein that in humans is encoded by the DRD1 gene. D1 receptors are the most ... "Regulation of transport of the dopamine D1 receptor by a new membrane-associated ER protein". Nat. Cell Biol. 3 (5): 492-8. doi ...
"BRSK2 is activated by cyclic AMP-dependent protein kinase A through phosphorylation at Thr260". Biochemical and Biophysical ... BR serine/threonine-protein kinase 2 is an enzyme that in humans is encoded by the BRSK2 gene. GRCh38: Ensembl release 89: ... "LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1". The EMBO Journal. 23 (4): 833- ... "Entrez Gene: BRSK2 BR serine/threonine kinase 2". Human BRSK2 genome location and BRSK2 gene details page in the UCSC Genome ...
"Negative regulation of histone deacetylase 8 activity by cyclic AMP-dependent protein kinase A". Mol. Cell. Biol. 24 (2): 765- ... The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase ... HDAC8+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... Gantt SL, Gattis SG, Fierke CA (2006). "Catalytic activity and inhibition of human histone deacetylase 8 is dependent on the ...
"Platelet glycoprotein Ib beta is phosphorylated on serine 166 by cyclic AMP-dependent protein kinase". J. Biol. Chem. 264 (26 ... GP1BB+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) PDBe-KB provides an overview of all ... Du X, Harris SJ, Tetaz TJ, Ginsberg MH, Berndt MC (July 1994). "Association of a phospholipase A2 (14-3-3 protein) with the ... Du X, Harris SJ, Tetaz TJ, Ginsberg MH, Berndt MC (1994). "Association of a phospholipase A2 (14-3-3 protein) with the platelet ...
... by the cyclic AMP-dependent protein kinase". The Journal of Biological Chemistry. 284 (30): 20070-8. doi:10.1074/jbc. ... this phosphorylation is mediated by cAMP-dependent protein kinase A (PKA). Second allosteric regulation is mediated by binding ... Leitges M, Gimborn K, Elis W, Kalesnikoff J, Hughes MR, Krystal G, Huber M (June 2002). "Protein kinase C-delta is a negative ... kinase-dependent Lyn/Tec/Dok-1 complex formation in hematopoietic cells". Blood. 96 (10): 3406-13. doi:10.1182/blood.V96.10. ...
"Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation". ... that are phosphorylated by a variety of protein kinases.[12][25] Ser40 is phosphorylated by the cAMP-dependent protein kinase.[ ... is phosphorylated by the calcium-calmodulin-dependent protein kinase.[27] MAPKAPK2 (mitogen-activated-protein kinase-activating ... Roskoski R, Roskoski LM (Jan 1987). "Activation of tyrosine hydroxylase in PC12 cells by the cyclic GMP and cyclic AMP second ...
cAMP-dependent protein kinase catalytic subunit gamma is an enzyme that in humans is encoded by the PRKACG gene. Cyclic AMP- ... 1998). "Effects of [D-Ala1] peptide T-NH2 and HIV envelope glycoprotein gp120 on cyclic AMP dependent protein kinases in normal ... "Characterization of the cAMP-dependent protein kinase catalytic subunit Cgamma expressed and purified from sf9 cells". Protein ... "Entrez Gene: PRKACG protein kinase, cAMP-dependent, catalytic, gamma". Marx, S O; Reiken S; Hisamatsu Y; Jayaraman T; Burkhoff ...
"Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation". ... that are phosphorylated by a variety of protein kinases. Ser40 is phosphorylated by the cAMP-dependent protein kinase. Ser19 ( ... is phosphorylated by the calcium-calmodulin-dependent protein kinase. MAPKAPK2 (mitogen-activated-protein kinase-activating ... "Molecular cloning of cDNA coding for brain-specific 14-3-3 protein, a protein kinase-dependent activator of tyrosine and ...
Huang W, Zhou X, Lefebvre V, de Crombrugghe B (June 2000). "Phosphorylation of SOX9 by cyclic AMP-dependent protein kinase A ... SOX9+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) SOX9 human gene location in the UCSC ... The Sox9 protein has been implicated in both initiation and progression of multiple solid tumors. Its role as a master ... Transcription factor SOX-9 is a protein that in humans is encoded by the SOX9 gene. SOX-9 recognizes the sequence CCTTGAG along ...
... two sites on glycogen synthetase phosphorylated by cyclic AMP-dependent protein kinase and their dephosphorylation by protein ... "A reinvestigation of the phosphorylation of rabbit skeletal-muscle glycogen synthase by cyclic-AMP-dependent protein kinase. ... protein kinase A (PKA), and casein kinase 2 (CK2). Each of these protein kinases lead to phosphorylated and catalytically ... Insulin stimulates glycogen synthase by inhibiting glycogen synthase kinases or/and activating protein phosphatase 1 (PP1) ...
... encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases ... Huang S, Li Q, Alberts I, Li X (2016). "PRKX, a Novel cAMP-Dependent Protein Kinase Member, Plays an Important Role in ... Protein kinase, X-linked is a protein that in humans is encoded by the PRKX gene. This gene ... a phylogenetically and functionally distinct cAMP-dependent protein kinase, activates renal epithelial cell migration and ...
Identification of four sites phosphorylated in intact cells and in vitro by cyclic AMP-dependent protein kinase and p34cdc2". ... Regulation of stathmin is cell cycle dependent and controlled by the cell's protein kinases in response to specific cell ... Maucuer A, Camonis JH, Sobel A (April 1995). "Stathmin interaction with a putative kinase and coiled-coil-forming protein ... "Serine 25 of oncoprotein 18 is a major cytosolic target for the mitogen-activated protein kinase". The Journal of Biological ...
Expression of the promoter is shown to be induce by phorbol esters and cyclic-AMP-dependent protein kinase signaling. The four ... due to phosphorylation of Ser-460 by PKA or AMP-dependent protein kinase. The high '2-Kase' activity of PFKFB3 is also due to ... September 2009). "Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases ... Manes NP, El-Maghrabi MR (June 2005). "The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase ...
"MTG8 proto-oncoprotein interacts with the regulatory subunit of type II cyclic AMP-dependent protein kinase in lymphocytes". ... Protein CBFA2T1 is a protein that in humans is encoded by the RUNX1T1 gene. The protein encoded by this gene is a putative zinc ... with serine/threonine protein kinases and heat shock protein HSP90 in human hematopoietic cell lines". Jpn. J. Cancer Res. 90 ( ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ...
... of the sites phosphorylated by cyclic AMP-dependent protein kinase on the beta 2 subunit of L-type voltage-dependent calcium ... Voltage-dependent L-type calcium channel subunit beta-2 is a protein that in humans is encoded by the CACNB2 gene. Mutation in ... Bünemann M, Gerhardstein BL, Gao T, Hosey MM (1999). "Functional regulation of L-type calcium channels via protein kinase A- ... GeneReviews/NIH/NCBI/UW entry on Brugada syndrome CACNB2+protein,+human at the US National Library of Medicine Medical Subject ...
Guild BC, Strominger JL (1984). "HLA-A2 antigen phosphorylation in vitro by cyclic AMP-dependent protein kinase. Sites of ... sequence formed by the fusion of ret tyrosine kinase and the regulatory subunit RI alpha of cyclic AMP-dependent protein kinase ... "A kinase anchoring protein (AKAP) interaction and dimerization of the RIalpha and RIbeta regulatory subunits of protein kinase ... cAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. cAMP ...
Mayeenuddin LH, Garrison JC (January 2006). "Phosphorylation of P-Rex1 by the cyclic AMP-dependent protein kinase inhibits the ... 5-trisphosphate-dependent Rac exchanger 1 protein is a protein that in humans is encoded by the PREX1 gene. The protein encoded ... March 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology. 3 (1): 89. ...
... or cyclic AMP-dependent protein kinase. 1992: Elected to the American Academy of Arts and Sciences 1996: Elected to the ... She is known for her research on protein kinases, particularly protein kinase A. She was elected to the Institute of Medicine ... Taylor's research group has focused on the structure and function of protein kinases, particularly protein kinase A, since ... "Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase". Science. 253 (5018): ...
... two sites on glycogen synthetase phosphorylated by cyclic AMP-dependent protein kinase and their dephosphorylation by protein ... "A reinvestigation of the phosphorylation of rabbit skeletal-muscle glycogen synthase by cyclic-AMP-dependent protein kinase. ... Amino acid sequence at the sites phosphorylated by glycogen synthase kinase-3, and extension of the N-terminal sequence ... "The muscle-specific protein phosphatase PP1G/R(GL)(G(M))is essential for activation of glycogen synthase by exercise". J Biol ...
... nuclear located protein kinase C and cyclic AMP-dependent protein kinase". Frontiers in Bioscience. 13 (13): 1206-26. doi: ... involved in calcium-mediated activation of protein kinase C;[76] the prostaglandins, which are one type of fatty-acid derived ... Protein-lipid interaction. *Phenolic lipid, a class of natural products composed of long aliphatic chains and phenolic rings ... Parodi AJ, Leloir LF (April 1979). "The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell". ...
... type-II regulatory subunit of cyclic-AMP-dependent protein kinase by glycogen synthase kinase 3 and glycogen synthase kinase 5 ... "Ezrin is a cyclic AMP-dependent protein kinase anchoring protein". The EMBO Journal. 16 (1): 35-43. doi:10.1093/emboj/16.1.35. ... "A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent ... Beebe SJ, Salomonsky P, Holroyd C, Becker D (Dec 1993). "Differential expression of cyclic AMP-dependent protein kinase ...
"Phosphorylation of SOX9 by cyclic AMP-dependent protein kinase A enhances SOX9's ability to transactivate a Col2a1 chondrocyte- ... SOX9 protein, human Medical Subject Headings (MeSH) na Biblioteca Nacional de Medicina dos EUA. ... "SOX9 interacts with a component of the human thyroid hormone receptor-associated protein complex". Nucleic Acids Res. 30 (14 ... "Direct interaction of SRY-related protein SOX9 and steroidogenic factor 1 regulates transcription of the human anti-Müllerian ...
Misra, K. and Pandey, S.C. (2006). The decreased cyclic-AMP dependent-protein kinase A function in the nucleus accumbens: a ... Some of the kinase families currently linked to alcoholism are Ca2+/calmodulin-dependent protein kinases (CaMKs), protein ... Ethanol enhances growth factor activation of mitogen-activated protein kinases by a protein kinase C-dependent mechanism. Proc ... Ethanol exposure alters the phosphorylation of cyclic AMP responsive element binding protein and cyclic AMP responsive element ...
... cyclic AMP or cAMP), which activates protein kinase A (cAMP-dependent protein kinase). This enzyme, in turn, activates ... The enzyme protein kinase A that was stimulated by the cascade initiated by glucagon will also phosphorylate a single serine ... The conformation change in the receptor activates G proteins, a heterotrimeric protein with α, β, and γ subunits. When the G ... When dissolved in a fluid state, glucagon can form amyloid fibrils, or tightly woven chains of proteins made up of the ...
... cyclic AMP or cAMP), which activates protein kinase A (cAMP-dependent protein kinase). This enzyme, in turn, activates ... cAMP binds to protein kinase A, and the complex phosphorylates phosphorylase kinase. Phosphorylated phosphorylase kinase ... The enzyme protein kinase A (PKA) that was stimulated by the cascade initiated by glucagon will also phosphorylate a single ... The transmembrane proteins interacts with Gɑβ𝛾. Gɑ separates from Gβ𝛾 and interacts with the transmembrane protein adenylyl ...
Through these studies, the cyclic AMP-dependent protein kinase A signaling has been identified as a key mediator of cortisol ... and the mutations associated with the dysregulation of cyclic AMP - protein kinase A pathways have been implicated in the ... The term "Cushing's disease" refers specifically to "secondary hypercortisolism" classified as "ACTH-dependent Cushing's ...
... collaboration with Paul Greengard resulted in demonstration that cAMP-dependent protein kinase, also known as protein kinase A ... By 1972 they had evidence that the second messenger molecule cyclic AMP (cAMP) was produced in Aplysia ganglia under conditions ... "Genetic evidence for a protein-kinase-A-mediated presynaptic component in NMDA-receptor-dependent forms of long-term synaptic ... are defective in mice carrying a targeted disruption of the gene encoding the RI beta subunit of cAMP-dependent protein kinase" ...
3',5'-cyclic-AMP phosphodiesterase. *Protein kinase A. cGMP:. *Transducin. *Gustducin. *Guanylate cyclase ... see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... GTP-binding protein regulators regulate G proteins in several different ways. Small GTPases act as molecular switches in ... and thus requires another class of regulatory proteins to accelerate this activity, the GTPase activating proteins (GAPs). ...
Also, it may be activated by a cAMP-dependent protein kinase (PKA). This pathway is significantly less effective than the first ... which is necessary for lipid mobilization in response to cyclic AMP, which itself is provided by the activation of Gs protein- ... protein binding. • hydrolase activity. • protein kinase binding. • serine hydrolase activity. Cellular component. • cytoplasm. ... protein phosphorylation. • long-chain fatty acid catabolic process. • metabolism. • triglyceride catabolic process. ...
3',5'-cyclic-AMP phosphodiesterase. *Protein kinase A. cGMP:. *Transducin. *Gustducin. *Guanylate cyclase ... "Direct binding of G-protein betagamma complex to voltage-dependent calcium channels". Nature. 385 (6615): 446-50. doi:10.1038/ ... protein complex binding. • signal transducer activity. • protein binding. • GTPase activity. • GTPase binding. • G-protein ... 1omw: Crystal Structure of the complex between G Protein-Coupled Receptor Kinase 2 and Heterotrimeric G Protein beta 1 and ...
ARPP-21: Cyclic AMP-regulated phosphoprotein, 21 kDa. *AZI2: encoding protein 5-azacytidine-induced protein 2 ... PIK3CA: phosphoinositide-3-kinase, catalytic, alpha polypeptide. *PROSER1: Proline and serine rich protein 1 ... CACNA2D3: calcium channel, voltage-dependent, alpha 2/delta subunit 3. *CCR5: chemokine (C-C motif) receptor 5 ... C3orf14-Chromosome 3 open reading frame 14: predicted DNA binding protein.. *C3orf23: encoding protein Uncharacterized protein ...
... nuclear located protein kinase C and cyclic AMP-dependent protein kinase". 》Frontiers in Bioscience》 13 (13): 1206-26. doi: ... Parodi AJ, Leloir LF (April 1979). "The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell". 》 ... The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis". 》Journal ... "Mitochondrial membrane biogenesis: phospholipids and proteins go hand in hand". 》The Journal of Cell Biology》 184 (4): 469-72 ...
Gαs activates the cAMP-dependent pathway by stimulating the production of cyclic AMP (cAMP) from ATP. This is accomplished by ... cAMP can then act as a second messenger that goes on to interact with and activate protein kinase A (PKA). PKA can ... Types of G protein signaling[edit]. G protein can refer to two distinct families of proteins. Heterotrimeric G proteins, ... G proteins, also known as guanine nucleotide-binding proteins, are a family of proteins that act as molecular switches inside ...
Protein kinase activationEdit. cGMP is involved in the regulation of some protein-dependent kinases. For example, PKG (protein ... is a cyclic nucleotide derived from guanosine triphosphate (GTP). cGMP acts as a second messenger much like cyclic AMP. Its ... Francis SH, Corbin JD (August 1999). "Cyclic nucleotide-dependent protein kinases: intracellular receptors for cAMP and cGMP ... cGMP; 3',5'-cyclic GMP; Guanosine cyclic monophosphate; Cyclic 3',5'-GMP; Guanosine 3',5'-cyclic phosphate ...
... and were eventually shown to encode components of an intracellular signaling pathway involving cyclic AMP, protein kinase A, ... Sepia colored eyes are not dependent on the sex of the fly. The Sepia eye color decreases sexual activity in males and ... Calcium binds to proteins such as calmodulin (CaM) and an eye-specific protein kinase C (PKC) known as InaC. These proteins ... For example, disruption of the interaction between InaC, the protein kinase C, and InaD results in a delay in inactivation of ...
... , more precisely known as adenosine 3',5'-monophosphate (cyclic AMP)-dependent protein kinase was discovered by ... "cAMP-dependent protein kinase" redirects here. It is not to be confused with AMP-activated protein kinase or cyclin-dependent ... Cyclic+AMP-Dependent+Protein+Kinases at the US National Library of Medicine Medical Subject Headings (MeSH) ... protein kinase A (PKA[N 1]) is a family of enzymes whose activity is dependent on cellular levels of cyclic AMP (cAMP). PKA is ...
Phosphorylation by cAMP-dependent protein kinasesEdit. Cyclic AMP-dependent protein kinases (protein kinase A) are activated by ... The G protein-coupled receptor kinases (GRKs) are protein kinases that phosphorylate only active GPCRs.[53] G-protein-coupled ... stimulative regulative G-protein (Gs) or inhibitory regulative G-protein (Gi); adenylyl cyclase; protein kinase A (PKA); and ... G-protein-dependent signalingEdit. There are three main G-protein-mediated signaling pathways, mediated by four sub-classes of ...
North B, Verdin E «Sirtuins: Sir2-related NAD-dependent protein deacetylases». Genome Biol, 5, 5, 2004, pàg. 224. DOI: 10.1186/ ... Sakuraba H, Kawakami R, Ohshima T «First archaeal inorganic polyphosphate/ATP-dependent NAD kinase, from hyperthermophilic ... Cyclic ADP-Ribose and NAADP: Structure, Metabolism and Functions. Kluwer Academic Publishers, 2002. ISBN 1-40207-281-3. ... AMP) al fosfat 5' d'un extrem del DNA. Aquest intermediari és seguidament atacat pel grup hidroxil 3' de l'altre extrem de DNA ...
Phosphorylation of a protein by a kinase can activate a cascade such as the mitogen-activated protein kinase cascade.[23] ... cyclic AMP, which is involved in triggering calcium signals by the release of calcium from intracellular stores.[24] This form ... Enzyme inhibitors of ATP-dependent enzymes such as kinases are needed to examine the binding sites and transition states ... Kinases are the most common ATP-binding proteins. They share a small number of common folds.[22] ...
3',5'-cyclic-AMP phosphodiesterase. *Protein kinase A. cGMP:. *Transducin. *Gustducin. *Guanylate cyclase ... see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... Guanylyl cyclase activator (protein). References[edit]. *^ Sakurai K.; Chen J.; Kefalov V. (2011). "Role of guanylate cylcase ... Guanylate cyclase is often part of the G protein signaling cascade that is activated by low intracellular calcium levels and ...
Francis SH, Corbin JD (1999). "Cyclic nucleotide-dependent protein kinases: intracellular receptors for cAMP and cGMP action". ... Djeluje kao sekundarni glasnik kao i ciklični AMP. Naprimjer, cGMP aktivira unutarćelijske protein kinaze u odgovoru na ... Monofosfati (AMP, GMP, UMP, CMP) • Difosfati (ADP, GDP, UDP, CDP) • Trifosfati (ATP, GTP, UTP, CTP) ... Cyclic guanosine monophosphate na US National Library of Medicine Medical Subject Headings (MeSH) ...
Carugo O, Argos P (1997). "NADP-dependent enzymes. I: Conserved stereochemistry of cofactor binding". Proteins 28 (1): 10-28. ... "First archaeal inorganic polyphosphate/ATP-dependent NAD kinase, from hyperthermophilic archaeon Pyrococcus horikoshii: cloning ... Guse AH (2004). "Biochemistry, biology, and pharmacology of cyclic adenosine diphosphoribose (cADPR)". Curr. Med. Chem. 11 (7 ... AMP) ao fosfato 5' dun extremo do ADN. Este intermediario é entón atacado polo grupo hidroxilo 3' do outro extremo de ADN, ...
... and glucose 6 phosphate may inhibit the phosphorylation of glycogen synthase by cyclic AMP-stimulated protein kinase.[7] ... For example, phosphorylating glucose is necessary for insulin-dependent mechanistic target of rapamycin pathway activity within ... Protein phosphorylationEdit. Main article: Protein phosphorylation. Protein phosphorylation is considered the most abundant ... Cohen, Philip (2002-05-01). "The origins of protein phosphorylation". Nature Cell Biology. 4 (5): E127-130. doi:10.1038/ncb0502 ...
... and adenyl cyclase converts AMP into cyclic AMP (cAMP) thereby down-regulating cAMP-responsive proteins involved in cell ... DAG activates certain protein kinase C enzymes )PKCs) that phosphorylate and thereby regulate target proteins involved in cell ... This control involves, at least in part, the DP2-dependent activation of the male germ cell marker Nanos2 and the inhibition of ... coupled to cyclic nucleotide second messenger. • chemotaxis. • positive regulation of G-protein coupled receptor protein ...
... upstream enhancer of the rat sodium/iodide symporter gene and participates in both thyroid-specific and cyclic-AMP-dependent ... Protein kinase A) activation and, as a result, the activation of the transcription factor Pax8 after phosphorylation. These two ... TSH first binds to its receptors which are joined to G proteins, and then induces the activation of the enzyme adenylate ... This ingested iodine is bound to serum proteins, especially to albumins.[citation needed] The rest of the iodine which remains ...
T cells by cyclic AMP-dependent signal transduction pathway.". Cytokine. 10 (11): 841-50. PMID 9878122. doi:10.1006/cyto. ... Expression of IL-17 in human memory CD45RO+ T lymphocytes and its regulation by protein kinase A pathway.". Cytokine. 11 (4): ... induced gene expression in articular chondrocytes is associated with activation of mitogen-activated protein kinases and NF- ... Laan M, Palmberg L, Larsson K, Lindén A (2002). „Free, soluble interleukin-17 protein during severe inflammation in human ...
3',5'-cyclic-AMP phosphodiesterase. *Protein kinase A. cGMP:. *Transducin. *Gustducin. *Guanylate cyclase ... function of cAMP-dependent protein kinase. In humans, cAMP works by activating protein kinase A (PKA, cAMP-dependent protein ... an enzyme called protein kinase A (PKA).[12]. The PKA enzyme is also known as cAMP-dependent enzyme because it gets activated ... "Multiple pathway signal transduction by the cAMP-dependent protein kinase". FASEB J. 8 (15): 1227-36. doi:10.1096/fasebj.8.15. ...
... s, when bound with the dependent kinases, such as the p34/cdc2/cdk1 protein, form the maturation-promoting factor. MPFs ... Heterotrimeric G protein *Gs/Gi. *Adenylate cyclase. *cAMP. *3',5'-cyclic-AMP phosphodiesterase ... is a family of proteins that control the progression of cells through the cell cycle by activating cyclin-dependent kinase (CDK ... Nurse won the 2001 Nobel Prize in Physiology or Medicine for their discovery of cyclin and cyclin-dependent kinase.[17] ...
... and glucose 6 phosphate may inhibit the phosphorylation of glycogen synthase by cyclic AMP-stimulated protein kinase.[12] ... In the late 1990s, it was recognized that phosphorylation of some proteins causes them to be degraded by the ATP-dependent ... Protein phosphorylation[edit]. Main article: Protein phosphorylation. Function[edit]. Reversible phosphorylation of proteins is ... Kinases phosphorylate proteins and phosphatases dephosphorylate proteins. Many enzymes and receptors are switched "on" or "off ...
It is a thiamine-pyrophosphate-dependent protein that catalyzes the oxidative decarboxylation of α-ketoglutarate substrate and ... Cofactor TPP and AMP are shown in cyan and blue, respectively. [17] (B) Diagram of the 12 subunit catalytic core of the cubic ... The citric acid cycle is a cyclic metabolic pathway involved in cellular respiration, which eventually converts carbohydrates, ... Aβ oligomers formed are found to be toxic since they can bind to different neurotransmitter receptors and thus stimulate kinase ...
It is hypothesized that the dual effects of lithium are due to the inhibition of G-proteins that mediate cyclic AMP production. ... "Definition of a metal-dependent/Li+-inhibited phosphomonoesterase protein family based upon a conserved three-dimensional core ... Gould, Todd; Picchini, Alyssa; Einat, Haim; Manji, Husseini (2006-11-01). "Targeting Glycogen Synthase Kinase-3 in the CNS: ... Lithium was found to increase the basal levels of cyclic AMP but impair receptor coupled stimulation of cyclic AMP production.[ ...
... nuclear located protein kinase C and cyclic AMP-dependent protein kinase. Frontiers in Bioscience. 2008, 13 (13): 1206-26. PMID ... The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell. Biochim. Biophys. Acta. April 1979, ... Mitochondrial membrane biogenesis: phospholipids and proteins go hand in hand. Journal of Cell Biology. 2009, 184 (4): 469-72. ... The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis. J. Lipid ...
Human cyclic AMP-dependent protein kinase PKA inhibitor complex. *DOI: 10.2210/pdb3MVJ/pdb ... cAMP-dependent protein kinase catalytic subunit alpha. A, B, E. 371. Homo sapiens. Mutation(s): 0 Gene Names: PKA, PKACA, ... cAMP-dependent protein kinase inhibitor alpha. I, J, K. 20. N/A. Mutation(s): 0 ...
There is a possibility that this protein is a physiological substrate of cyclic AMP-dependent protein kinase and that the ... An endogenous polysomal cyclic AMP-dependent protein kinase specifically phosphorylates a 150,000-dalton peptide bound to an ... Endogenous substrate for cyclic AMP-dependent protein kinase in adrenocortical polyadenylated messenger ribonucleoproteins ... Endogenous substrate for cyclic AMP-dependent protein kinase in adrenocortical polyadenylated messenger ribonucleoproteins ...
Cyclic AMPdependent protein kinase explanation free. What is Cyclic AMPdependent protein kinase? Meaning of Cyclic AMPdependent ... protein kinase medical term. What does Cyclic AMPdependent protein kinase mean? ... Looking for online definition of Cyclic AMPdependent protein kinase in the Medical Dictionary? ... cAMP-dependent protein kinase. (redirected from Cyclic AMPdependent protein kinase) cAMP-dependent protein kinase. a tetrameric ...
... of cAMP-dependent protein kinase, and studies on the rates of transcription from the gene coding for this protein, showed that ... The regulation of the levels of cAMP-dependent protein kinases during myogenesis in L6 was studied, as these enzymes have been ... Studies on the regulation of the catalytic subunit (C) of cAMP-dependent protein kinase showed that the increase in this ... Lorimer, Ian Anthony, "Regulation Of Cyclic-amp-dependent Protein Kinases During Skeletal Myogenesis" (1989). Digitized Theses ...
... of cyclic AMP and defective glucose repression in yeast strains with reduced activity of cyclic AMP-dependent protein kinase. ... Cyclic AMP-Dependent Protein Kinase Controls Virulence of the Fungal Pathogen Cryptococcus neoformans Cletus A. DSouza, J. ... 1999) Cyclic AMP-dependent protein kinase regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Mol. Cell. Biol. ... ThePKA1 gene encoding the major cyclic AMP (cAMP)-dependent protein kinase catalytic subunit was identified and disrupted.pka1 ...
Ca2+-phospholipid-dependent and cyclic AMP-dependent protein kinases in mouse pancreatic islets. P Thams, K Capito, C J ... Endogenous substrate proteins for Ca2+-calmodulin-dependent, Ca2+-phospholipid-dependent and cyclic AMP-dependent protein ... Endogenous substrate proteins for Ca2+-calmodulin-dependent, Ca2+-phospholipid-dependent and cyclic AMP-dependent protein ... Endogenous substrate proteins for Ca2+-calmodulin-dependent, Ca2+-phospholipid-dependent and cyclic AMP-dependent protein ...
These peptides bind to cell surface receptors to activate the cyclic AMP (cAMP) signaling pathway leading to an increase of ... Cyclic AMP stimulates SF-1-dependent CYP11A1 expression through homeodomain-interacting protein kinase 3-mediated Jun N- ... These peptides bind to cell surface receptors to activate the cyclic AMP (cAMP) signaling pathway leading to an increase of ... Investigating the factors participating in SF-1 action, we found that c-Jun and homeodomain-interacting protein kinase 3 (HIPK3 ...
An enhancer element and a functional cyclic AMP-dependent protein kinase are required for expression of adrenocortical 21- ... An enhancer element and a functional cyclic AMP-dependent protein kinase are required for expression of adrenocortical 21- ... An enhancer element and a functional cyclic AMP-dependent protein kinase are required for expression of adrenocortical 21- ...
Restricted efflux of the type II isoform of cyclic AMP-dependent protein kinase in permeabilized rat hepatocytes. Olav K. ... Restricted efflux of the type II isoform of cyclic AMP-dependent protein kinase in permeabilized rat hepatocytes ... Restricted efflux of the type II isoform of cyclic AMP-dependent protein kinase in permeabilized rat hepatocytes ... Restricted efflux of the type II isoform of cyclic AMP-dependent protein kinase in permeabilized rat hepatocytes ...
Regulation of the Subcellular Localization of Cyclic AMP-Dependent Protein Kinase in Response to Physiological Stresses and ... Regulation of the Subcellular Localization of Cyclic AMP-Dependent Protein Kinase in Response to Physiological Stresses and ... Regulation of the Subcellular Localization of Cyclic AMP-Dependent Protein Kinase in Response to Physiological Stresses and ... Regulation of the Subcellular Localization of Cyclic AMP-Dependent Protein Kinase in Response to Physiological Stresses and ...
extracellular signal-regulated kinase. PKA. cyclic AMP-dependent protein kinase. WT. wild-type. CGP-12177. 4-[3-[(1,1- ... β2-Adrenergic Receptor Lacking the Cyclic AMP-Dependent Protein Kinase Consensus Sites Fully Activates Extracellular Signal- ... β2-Adrenergic Receptor Lacking the Cyclic AMP-Dependent Protein Kinase Consensus Sites Fully Activates Extracellular Signal- ... β2-Adrenergic Receptor Lacking the Cyclic AMP-Dependent Protein Kinase Consensus Sites Fully Activates Extracellular Signal- ...
cAMP-dependent PKA is an ubiquitous serine/theonine protein kinase present in a variety of tissues (e.g. brain, skeletal muscle ... Cyclic AMP Dependent Protein Kinase A Regulatory Subunit 1a, Recombinant (cAMP PKA R1) ... Customer review for "Cyclic AMP Dependent Protein Kinase A Regulatory Subunit 1a, Recombinant (cAMP PKA R1)" ... Product information "Cyclic AMP Dependent Protein Kinase A Regulatory Subunit 1a, Recombinant (cAMP PKA R1)" ...
... dependent kinase and phosphatase, cyclic AMPdependent protein kinase (PKA) and calcineurin (also called protein phosphatase 2B ... Ser 656 is phosphorylated by cyclic AMPdependent protein kinase and dephosphorylated by calcineurin, and its phosphorylation ... Reversible phosphorylation of Drp1 by cyclic AMPdependent protein kinase and calcineurin regulates mitochondrial fission and ... Drp1, dynamin‐related protein 1; GFP, green fluorescent protein; PKA, cAMP‐dependent protein kinase, pS40 TH, phospho‐Ser 40 ...
These results suggest that both protein kinase C and tyrosine kinase are involved in melanin production by cyclic AMP-dependent ... Melanin production and tyrosinase activity by MSH are significantly inhibited by cyclic AMP-dependent protein kinase inhibitor ... calmodulin-dependent protein kinase II inhibitor), PD098059 (mitogen-activated protein kinase inhibitor, MAPKK) and worthmannin ... KT5720) and protein kinase C down-regulation treated with PMA. Bisindolmaleimide (1,TEX,$\mu$,/TEX,M), protein kinase C ...
Effect of indomethacin on the cyclic AMP-dependent protein kinase. , European journal of pharmacology , 5/2/1980 ... Effect of indomethacin on the cyclic AMP-dependent protein kinase. R E Catalán M D Aragones A M Martinez M Armijo M Piña 5/2/ ... Catalán RE, Aragones MD, Martinez AM, Armijo M, Piña M. Effect of indomethacin on the cyclic AMP-dependent protein kinase. Eur ... Indomethacin inhibited cyclic AMP-dependent protein kinase activity in small intestine in in vivo experiments. An inverse ...
Vulliet PR, Langan TA, Weiner N. Tyrosine hydroxylase: A substrate of cyclic AMP-dependent protein kinase. Proceedings of the ... Vulliet, P. R., Langan, T. A., & Weiner, N. (1980). Tyrosine hydroxylase: A substrate of cyclic AMP-dependent protein kinase. ... Vulliet, Philip R ; Langan, T. A. ; Weiner, N. / Tyrosine hydroxylase : A substrate of cyclic AMP-dependent protein kinase. In ... Tyrosine hydroxylase : A substrate of cyclic AMP-dependent protein kinase. / Vulliet, Philip R; Langan, T. A.; Weiner, N. ...
Berglund, L. (1978). Phosphorylation of rat kidney pyruvate kinase type L by cyclic 3′,5′-amp-dependent protein kinase. BBA - ... Berglund, L 1978, Phosphorylation of rat kidney pyruvate kinase type L by cyclic 3′,5′-amp-dependent protein kinase, BBA - ... Phosphorylation of rat kidney pyruvate kinase type L by cyclic 3′,5′-amp-dependent protein kinase. BBA - Enzymology. 1978 May ... Phosphorylation of rat kidney pyruvate kinase type L by cyclic 3′,5′-amp-dependent protein kinase. In: BBA - Enzymology. 1978 ...
The cAMP-dependent protein kinase and cGMP-dependent protein kinase are major intrace … ... Cyclic AMP-Dependent Protein Kinases / metabolism* * Cyclic GMP / metabolism* * Cyclic GMP-Dependent Protein Kinases / ... The cAMP-dependent protein kinase and cGMP-dependent protein kinase are major intracellular receptors for these nucleotides, ... Cyclic nucleotide-dependent protein kinases: intracellular receptors for cAMP and cGMP action Crit Rev Clin Lab Sci. 1999 Aug; ...
Expression of cyclic AMP-dependent protein KINASE-I isoforms in the human prostate: Relation to smooth muscle alpha-actin and ... HomeResearch Outputs Expression of cyclic AMP-dependent protein KINASE-I isoforms... ...
... cyclase 6 mediates the action of cyclic AMP-dependent secretagogues in mouse pancreatic exocrine cells via protein kinase A ... cyclase 6 mediates the action of cyclic AMP-dependent secretagogues in mouse pancreatic exocrine cells via protein kinase A ... cyclase 6 mediates the action of cyclic AMP-dependent secretagogues in mouse pancreatic exocrine cells via protein kinase A ... cyclase 6 mediates the action of cyclic AMP-dependent secretagogues in mouse pancreatic exocrine cells via protein kinase A ...
Wound-healing effect of ginsenoside Rd from leaves of Panax ginseng via cyclic AMP-dependent protein kinase pathway. In: ... Wound-healing effect of ginsenoside Rd from leaves of Panax ginseng via cyclic AMP-dependent protein kinase pathway. European ... Wound-healing effect of ginsenoside Rd from leaves of Panax ginseng via cyclic AMP-dependent protein kinase pathway. / Kim, ... title = "Wound-healing effect of ginsenoside Rd from leaves of Panax ginseng via cyclic AMP-dependent protein kinase pathway", ...
title = "Cyclic AMP-dependent protein kinase activity in Trypanosoma cruzi",. abstract = "A cyclic AMP-dependent protein kinase ... In addition, a cyclic AMP-binding protein co-eluted with the second kinase activity peak. Cyclic AMP-dependent protein kinase ... In addition, a cyclic AMP-binding protein co-eluted with the second kinase activity peak. Cyclic AMP-dependent protein kinase ... In addition, a cyclic AMP-binding protein co-eluted with the second kinase activity peak. Cyclic AMP-dependent protein kinase ...
... cyclic AMP-dependent protein kinase; cGK, cyclic GMP-dependent protein kinase; FAK, focal adhesion kinase; GBSS, Geys balanced ... VASP is a prominent substrate for both cGMP-dependent protein kinase (cGK) and cAMP-dependent protein kinase. Evidence ... VASP is phosphorylated by cAMP-dependent protein kinase (cAK) or cGMP-dependent protein kinase (cGK). The phosphorylation of ... Cyclic GMP-dependent protein kinase is required for thrombospondin and tenascin mediated focal adhesion disassembly. J. Cell ...
Cyclic AMP-Dependent Protein Kinases/chemistry. *Cyclic AMP-Dependent Protein Kinases/genetics ... cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes ... Molecular mechanisms controlling the localisation of protein kinase A.. Griffioen G1, Thevelein JM. ...
... is a paradigm for the mechanisms of ligand-dependent and allosteric regulation in signalling. Here we report the orthologous ... Protein kinase A (PKA) is typically activated by cAMP. Here, Bachmaier et al. show that PKA of Trypanosoma is activated by ... The existence of eukaryotic CNB domains not associated with binding of cyclic nucleotides suggests that orphan CNB domains in ... subunit and its unique C-terminal αD helix account for this ligand swap between trypanosome PKA and canonical cAMP-dependent ...
... rather than cAMP-protein kinase A, mediates GSIS potentiation via OLFR15. These findings suggest that the OR system in ... experiments using inhibitors and siRNA revealed that the pathway dependent on phospholipase C-inositol triphosphate, ... OLFR15-dependent GSIS enhancement is independent of the cyclic AMP (cAMP)-protein kinase A (PKA) pathway. We next endeavored to ... concanavalin A, an ER marker protein. (g) The expressions of OLFR15 protein in MIN6 cells and murine islets, and the ...
Roles of the AMP-activated and cyclic-AMP-dependent protein kinases in the adrenaline-induced inactivation of acetyl-CoA ... phosphorylated by cyclic-AMP-dependent protein kinase) and Ser-79 (phosphorylated by the AMP-activated protein kinase). ... which is an antagonist of binding of cyclic AMP to the regulatory subunit of cyclic-AMP-dependent protein kinase, opposes the ... Roles of the AMP-activated and cyclic-AMP-dependent protein kinases in the adrenaline-induced inactivation of acetyl-CoA ...
The effect of cyclic AMP (cAMP)-dependent protein phosphorylation on gamma-aminobutyric acidA (GABAA) receptor function was ... Cyclic AMP-dependent protein kinase decreases gamma-aminobutyric acidA receptor-mediated 36Cl- uptake by brain microsacs. ... Cyclic AMP-dependent protein kinase decreases gamma-aminobutyric acidA receptor-mediated 36Cl- uptake by brain microsacs.. ... The effect of cyclic AMP (cAMP)-dependent protein phosphorylation on gamma-aminobutyric acidA (GABAA) receptor function was ...
Retinoid acid increases cyclic amp-dependent protein kinase activity in b16-f1 mouse melanoma cells. Abstr. ... Niles, R M. and Logue, M P., "Retinoid acid increases cyclic amp-dependent protein kinase activity in b16-f1 mouse melanoma ...
  • These findings define a Gα protein-cAMP-PKA signaling pathway regulating differentiation and virulence of a human fungal pathogen. (asm.org)
  • These peptides bind to cell surface receptors to activate the cyclic AMP (cAMP) signaling pathway leading to an increase of steroidogenic gene expression. (nih.gov)
  • A signaling pathway parallel to the cAMP pathway, which includes a MAP kinase and the Ste12 transcription factor, has a major influence on filamentous growth in S. cerevisiae , while the role of a similar pathway in C. albicans appears less important for morphogenesis (reviewed in E rnst 2000 ). (genetics.org)
  • Together with ZDS1, provides a negative feedback control on the cell wall integrity-signaling pathway by acting as a negative regulator of MAP kinase SLT2/MPK1. (uniprot.org)
  • Filamentous growth requires at least two signaling pathways: the pheromone responsive MAP kinase cascade and the Gpa2p-cAMP-PKA signaling pathway. (genetics.org)
  • 50 μmol/L for COX-2 ( 3 , 4 )), impaired activation of activator protein-1 and the NFκB signaling pathway, resulting in reduced expression of COX-2 ( 13 , 20 ) and microsomal PGE 2 synthase-1 ( 11 ), might be responsible. (aacrjournals.org)
  • The Wnt proteins are palmitoylated secreted glycoproteins that activate the Wnt signaling pathway. (aacrjournals.org)
  • The Wnt signaling pathway is partly regulated by Wnt antagonists, including members of the Dickkopf and secreted Frizzled-related protein (sFRP) families, and Wnt inhibitory factor 1 ( 6 , 7 ). (aacrjournals.org)
  • sFRPs are modulators of the Wnt signaling pathway, interact with Wnt proteins, and prevent Frizzled receptors from binding to Wnt proteins, thereby downregulating Wnt signaling ( 8 ). (aacrjournals.org)
  • This cis element is also the target for repression by the cyclic AMP (cAMP)-signaling pathway. (asm.org)
  • Down regulation of the cyclic AMP (cAMP)-signaling pathway seems to be an important controlling factor of this transition. (asm.org)
  • These proteins, ropporin (a protein previously shown to interact with the Rho signaling pathway) and AKAP-associated sperm protein, are 39% identical to each other and share a strong sequence similarity with the conserved domain on the N terminus of RII that is involved in dimerization and AKAP binding. (embl-heidelberg.de)
  • a tetrameric protein composed of two regulatory subunits that bind cAMP, and two catalytic subunits that catalyze the transfer of a phosphoryl group from ATP to a target enzyme. (thefreedictionary.com)
  • Free Catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins. (biomol.com)
  • The same preparation was analysed by Western blotting, by using polyclonal antibodies to the regulatory subunits of protein kinases type I or II. (elsevier.com)
  • In Saccharomyces cerevisiae, as in higher eucaryotes, cyclic AMP (cAMP)-dependent protein kinase is a tetramer composed of two catalytic (C) subunits and two regulatory (R) subunits. (cshl.edu)
  • Neurofilaments (NFs) in the adult mammalian nervous system consist of three major subunits: low molecular weight neurofilament protein NF-L (68 kDa), NF-M (95 kDa), and NF-H (115 kDa) ( Liem, 1993 ). (jneurosci.org)
  • This diagram displays the protein subunits (blue) of the complex and how they interact with each other. (yeastgenome.org)
  • Induction of adenosine 3', 5'monophosphate-dependent protein kinase subunits during adipogenesis in vitro. (springer.com)
  • Identification and differential expression of two forms of regulatory subunits (RH) of cAMP-dependent protein kinase II in Friend erythroleukemic cells. (springer.com)
  • Conclusions- PKG positively regulates proteasome activities and proteasome-mediated degradation of misfolded proteins, likely through posttranslational modifications to proteasome subunits. (ahajournals.org)
  • In this study, the cellular compartmentalization and biochemical localization of regulatory subunits (R-subunits) of cyclic AMP-dependent protein kinase (cAPK) of ventricle heart tissue obtained from space-flown rats were determined. (nasa.gov)
  • Photoaffinity labeling with a 32P-8-azido analog of cyclic AMP, and electrophoretic separation of the proteins, was followed by autoradiographic identification of the subcellular fraction in which the labeled R subunits are localized. (nasa.gov)
  • Correspondingly, two alpha subunits of PTX -sensitive G proteins , Galphai2 and Galphai3, were identified by Western blotting . (ndif.org)
  • Here we studied the impact of the heterotrimeric G protein and the cAMP-mediated signaling network, including the regulatory subunits of the cAMP-dependent protein kinase (PKA), to study their effect on colony morphology, sexual development and regulation of bikaverins, fusarubins and GAs. (nih.gov)
  • Cyclic-AMP-dependent protein kinase consists of a regulatory dimer bound to two catalytic subunits. (uio.no)
  • The distribution and dissociation of cyclic adenosine 3':5'-monophosphate-dependent protein kinases in adipose, cardiac, and other tissues. (springer.com)
  • Amino acid sequence of the regulatory subunit of bovine type I adenosine cyclic 3',5'-phosphate dependent protein kinase. (springer.com)
  • Takio K, Smith SB, Krebs EG, Walsh KA, Titani K. Amino acid sequence of the regulatory subunit of bovine type II adenosine cyclic 3',5'-phosphate dependent protein kinase. (springer.com)
  • Hartl FT, Roskoski R, Jr. Cyclic adenosine 3':5'-monophosphate-dependent protein kinase. (springer.com)
  • For example, the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) pathways appear to participate in neuronal allocation. (wikipedia.org)
  • In C. albicans, the ability to switch between yeast and hyphal forms is thought to be a key virulence factor and is regulated by multiple signaling cascades-including the cyclic adenosine monophosphate/protein kinase A (cAMP/PKA), calcineurin, high-osmolarity glycerol (HOG), and mitogen-activated protein kinases (MAPK) signaling pathways-upon receiving environmental cues. (mdpi.com)
  • AdC, in turn, elevates levels of the metabolic regulator, cyclic adenosine monophosphate ( cAMP ). (ndif.org)
  • Interactions between cyclic adenosine monophosphate (cAMP) and Ca(2+) are widespread, and for both intracellular messengers, their spatial organization is important. (nih.gov)
  • Antiserum against the catalytic subunit of adenosine 3′:5′-cyclic monophosphate-dependent protein kinase. (biochemj.org)
  • Here we report a novel role for epinephrine and cyclic adenosine monophosphate (cAMP) in the regulation of human SS RBC adhesiveness via the laminin receptor, basal cell adhesion molecule/Lutheran (BCAM/Lu). (bloodjournal.org)
  • The cAMP-dependent protein kinase and cGMP-dependent protein kinase are major intracellular receptors for these nucleotides, and the actions of these enzymes account for much of the cellular responses to increased levels of cAMP or cGMP. (nih.gov)
  • Both secretin and vasoactive intestinal polypeptide (VIP) receptors are responsible for the activation of adenylyl cyclases (ACs), which increase intracellular cyclic AMP (cAMP) levels in the exocrine pancreas. (elsevier.com)
  • These results provide evidence that the catalytic subunit of PKA inhibits the function of brain GABAA receptors and demonstrate that this functional change is concomitant with an increase in protein phosphorylation. (muscimol.xyz)
  • The PKA, PKC, and protein phosphatase-2B/calcineurin (CaN) scaffold protein A-kinase anchoring protein (AKAP) 79 is localized to excitatory neuronal synapses where it is recruited to glutamate receptors by interactions with membrane-associated guanylate kinase (MAGUK) scaffold proteins. (rupress.org)
  • Organized around these PSD scaffolds are membrane receptors including N -methyl- d -aspartate (NMDA) and α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) ionotropic glutamate receptors as well as intracellular signaling proteins. (rupress.org)
  • However, in mouse cortical neuron cultures, prior to synaptogenesis, neither cAMP nor dopamine, which acts via cAMP, stimulated CREB-dependent gene transcription when NR2B-containing NMDA receptors (NMDARs) were blocked. (jneurosci.org)
  • Neuronal levels of cAMP are regulated by G-protein-coupled receptors linked to adenylyl cyclase (AC). (jneurosci.org)
  • Here, we report that, in immature neurons from embryonic mouse cortex, prior to synaptogenesis, DA and cAMP do not stimulate CREB-dependent gene transcription when NMDA receptors (NMDARs) are blocked. (jneurosci.org)
  • The dopamine D 1 receptor belongs to the superfamily of heptahelical receptors that modulate the activity of effectors such as adenylate cyclase by activation of specific heterotrimeric GTP-binding proteins (G proteins). (aspetjournals.org)
  • Although this model closely describes the regulation of some GPCRs, the regulation of other receptors differs significantly in ways that may be both cell- and receptor-dependent ( Koenig and Edwardson, 1996 ). (aspetjournals.org)
  • The enzyme is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G-protein-coupled receptors and is involved in a wide range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation. (biologists.org)
  • The primary research focus of our laboratory is to understand the signaling pathways induced by G-protein coupled receptors (GPCRs), particularly the role of regulator of G-protein signal (RGS) proteins in leukocyte trafficking and the pathogenesis of asthma and allergic diseases. (nih.gov)
  • A family of serine-threonine kinases that are specific for G-PROTEIN-COUPLED RECEPTORS. (curehunter.com)
  • Frizzled receptors, G protein-coupled receptors, are the major proteins that bind to Wnt proteins on the plasma membrane and trigger Wnt pathway signaling, which leads to activation of T-cell factor (TCF)/lymphoid enhancer factor (LEF) transcription. (aacrjournals.org)
  • G protein-coupled receptor activation leads to the membrane recruitment and activation of G protein-coupled receptor kinases, which phosphorylate receptors and lead to their inactivation. (duke.edu)
  • abstract = "Pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) type L was partly purified from rat kidney. (elsevier.com)
  • abstract = "A cyclic AMP-dependent protein kinase activity from epimastigote forms of Trypanosoma cruzi was characterized. (elsevier.com)
  • Melanin production and tyrosinase activity by MSH are significantly inhibited by cyclic AMP-dependent protein kinase inhibitor (KT5720) and protein kinase C down-regulation treated with PMA. (koreascience.or.kr)
  • Bisindolmaleimide (1 $\mu$ M), protein kinase C inhibitor, significantly inhibited melanin production and tyrosinase activity stimulated by MSH, forskolin and 8-Br-cAMP with the following order of potency: MSH>forskolin>8-Br-cAMP. (koreascience.or.kr)
  • Tyrosine kinase inhibitor, genistein and DHC, significantly inhibited both, but the inhibitory effect was more potent in 8-Br-cAMP-stimulated B16 cells than MSH-stimulated cells. (koreascience.or.kr)
  • Neither melanin production nor tyrosinase activity induced by MSH, forskolin and 8-Br-cAMP were affected by KN-62 (calmodulin-dependent protein kinase II inhibitor), PD098059 (mitogen-activated protein kinase inhibitor, MAPKK) and worthmannin (phosphatidylinositol 3-kinase inhibitor). (koreascience.or.kr)
  • All of these beneficial effects are associated with an induction of intracellular cAMP levels and phosphorylated cAMP response element-binding protein expression in nucleus, which both attenuated by adenine 9-β-d-arabinofuranoside, an adenylate cyclase inhibitor. (elsevier.com)
  • Evidence suggested that cGK regulated neutrophil spreading, as both VASP phosphorylation and neutrophil spreading were inhibited by Rp-8-pCPT-cGMPS (cGK inhibitor), but not KT5720 (cAMP-dependent protein kinase inhibitor). (jimmunol.org)
  • Inhibition of protein phosphatase-1 via phosphorylation of inhibitor-1 is therefore unlikely to be the mechanism by which cyclic-AMP-dependent protein kinase indirectly increases phosphorylation of acetyl-CoA carboxylase. (duke.edu)
  • Using a synthetic KSPXXXK peptide to screen for a specific kinase, we fractionated rat brain extracts by column chromatography and identified extracellular signal-regulated kinase (Erk2) activated by an upstream activator, the mitogen-activated protein kinase kinase MAPKK (MEK), by Western blot analysis, sequence identification, and inhibition by a specific MEK inhibitor (PD 98059). (jneurosci.org)
  • The MEK inhibitor PD 98059 also inhibited phosphorylation of NF-H, NF-M, and microtubule-associated protein (MAP) in primary rat hippocampal cells and caused a decrease in neurite outgrowth, suggesting that Erk1,2 may play an important role in neurite growth and branching. (jneurosci.org)
  • Pretreatment with the protein kinase A inhibitor H-89 ( N -[2-( p -bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide) or substitution of Ala for Thr268 reduced agonist-stimulated phosphorylation of the receptor and resulted in diminished trafficking of the receptor to the perinuclear region of the cell. (aspetjournals.org)
  • Functionally active targeting domain of the beta-adrenergic receptor kinase: an inhibitor of G beta gamma-mediated stimulation of type II adenylyl cyclase. (duke.edu)
  • 1986) "A Potent Synthetic Peptide Inhibitor of the cAMP-Dependent Protein Kinase" The Journal of Biological Chemistry261(3):989-992. (patentgenius.com)
  • 1985) "An Active Twenty-Amino-Acid-Residue Peptide Derived From the Inhibitor Protein of the Cyclic AMP-Dependent Protein Kinase" Biochem J. 231:655-661. (patentgenius.com)
  • 1991) "Protein Kinase C Substrate and Inhibitor Characteristics of Peptides Derived From the Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) Protein Phosphorylation Site Domain" The Journal of Biological Chemistry266(22):14390-14398. (patentgenius.com)
  • homologous to AKAP95 (HA95) and A kinase interacting protein (AKIP) and last is the protein kinase inhibitor (PKI) which is located both to the cytosol and the nucleus. (uio.no)
  • Muscimol-stimulated 36Cl- uptake was studied in mouse brain microsacs permeabilized to introduce the catalytic subunit of cAMP-dependent protein kinase (PKA). (muscimol.xyz)
  • The regulatory (R) subunit of cAMP-dependent protein kinase from the yeast Saccharomyces cerevisiae was expressed in Escherichia coli by engineering the gene for yeast R, BCY1, in an E. coli expression vector that contained a promoter from phage T7. (cshl.edu)
  • Genetic characterization of a brain-specific form of the type I regulatory subunit of cAMP-dependent protein kinase. (springer.com)
  • The neural type II regulatory subunit of cAMP-dependent protein kinase is present and regulated by hormones in the rat ovary. (springer.com)
  • v.13 Mitogen-activated protein kinase pathway and AP-1 are activated during cAMP-induced melanogenesis in B-16 melanoma cells Englaro, W.;Rezzonico, R.;Durand-Clement, M.;Lallemand, D.;Ortinne, J. P.;Ballotti, R. (koreascience.or.kr)
  • Here we have investigated which protein phosphatase activities can dephosphorylate recombinant τ phosphorylated with mitogen-activated protein kinase, glycogen synthase kinase-3β, neuronal cdc2-like kinase, or cyclic AMP-dependent protein kinase. (wiley.com)
  • In response to hormone- induced high cAMP levels, PKA phosphorylates glycogen synthetase (inhibition of the enzyme activity) and phosphorylase kinase to block glycogen synthesis. (biomol.com)
  • In the absence of "canonical" catenin-dependent Wnt signaling, cytoplasmic β-catenin is maintained at low levels via phosphorylations of S45 and S33, S37 and T41 by, respectively, casein kinase-1 and glycogen synthase kinase-3 (GSK-3) ( 1 , 2 ). (pnas.org)
  • Here we show that glycogen synthase kinase 3β (GSK-3β) is required for radiation-induced hippocampal neuronal apoptosis and subsequent neurocognitive decline. (aacrjournals.org)
  • One of possible neuroprotective mechanisms of lithium lies in its ability to inhibit glycogen synthase kinase 3β (GSK-3β), acting both by displacing magnesium and by indirectly inhibiting an activating phosphatase ( 10 ). (aacrjournals.org)
  • Glycogen synthase kinase 3 (GSK-3) is a multifunctional serine/threonine kinase found in all eukaryotes. (biologists.org)
  • Glycogen synthase kinase 3β (GSK 3β), a multifunctional serine and threonine kinase, plays a critical role in a variety of cellular activities, including signaling transduction, protein and glycogen metabolism, cell proliferation, cell differentiation, and apoptosis. (spandidos-publications.com)
  • Grimes CA and Jope RS: The multifaceted roles of glycogen synthase kinase 3beta in cellular signaling. (spandidos-publications.com)
  • Embi N, Rylatt DB and Cohen P: Glycogen synthase kinase-3 from rabbit skeletal muscle. (spandidos-publications.com)
  • Woodgett JR: Molecular cloning and expression of glycogen synthase kinase-3/factor A. EMBO J. 9:2431-2438. (spandidos-publications.com)
  • Thotala DK and Yazlovitskaya EM: GSK3B (glycogen synthase kinase 3 beta). (spandidos-publications.com)
  • Jope RS and Johnson GV: The glamour and gloom of glycogen synthase kinase-3. (spandidos-publications.com)
  • Fang X, Yu SX, Lu Y, Bast RC Jr, Woodgett JR and Mills GB: Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Proc Natl Acad Sci USA. (spandidos-publications.com)
  • Cross DA, Alessi DR, Cohen P, Andjelkovich M and Hemmings BA: Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature. (spandidos-publications.com)
  • Summers SA, Kao AW, Kohn AD, Backus GS, Roth RA, Pessin JE and Birnbaum MJ: The role of glycogen synthase kinase 3beta in insulin-stimulated glucose metabolism. (spandidos-publications.com)
  • Amar S, Belmaker RH and Agam G: The possible involvement of glycogen synthase kinase-3 (GSK-3) in diabetes, cancer and central nervous system diseases. (spandidos-publications.com)
  • Jope RS, Yuskaitis CJ and Beurel E: Glycogen synthase kinase-3 (GSK3): Inflammation, diseases, and therapeutics. (spandidos-publications.com)
  • Luo J: Glycogen synthase kinase 3beta (GSK3beta) in tumorigenesis and cancer chemotherapy. (spandidos-publications.com)
  • Naito S, Bilim V, Yuuki K, Ugolkov A, Motoyama T, Nagaoka A, Kato T and Tomita Y: Glycogen synthase kinase-3beta: A prognostic marker and a potential therapeutic target in human bladder cancer. (spandidos-publications.com)
  • Tubulin-dependent suppression of ΔΨ m is further potentiated by protein kinase A activation and glycogen synthase kinase-3β inhibition. (aspetjournals.org)
  • These protein kinases are necessary for normal accumulation/degradation of trehalose and glycogen, and the lack of these genes reduces glucose uptake and glycogen synthesis. (asm.org)
  • The PKA pathway was also shown to function downstream of the Gα protein Gpa1 and to regulate cAMP production by feedback inhibition. (asm.org)
  • v.13 Inhibition of the phosphatidylinositol 3-kinase/p70(S6)-kinase pathway induces B16 melanoma cells differentiation Busca, R.;Bertolotto, C.;Ortonne, J. P.;Ballotti, R. (koreascience.or.kr)
  • 4. As shown by okadaic acid inhibition, greater than 95% of the acetyl-CoA carboxylase phosphatase activity in extracts of rat adipocytes or liver is accounted for by protein phosphatase-2A, with less than 5% attributable to protein phosphatase-1. (duke.edu)
  • Inhibition of GSK-3β either by small molecules (SB216763 or SB415286) or by ectopic expression of kinase-inactive GSK-3β before irradiation significantly attenuated radiation-induced apoptosis in hippocampal neurons. (aacrjournals.org)
  • The aim of this study was to use pharmacological inhibition of protein kinase A and mutation of potential protein kinase A phosphorylation sites to determine the role of protein kinase A-catalyzed phosphorylation of the dopamine D 1 receptor in agonist-stimulated desensitization and internalization of the receptor. (aspetjournals.org)
  • It has recently been demonstrated that inhibition of cAMP-dependent protein kinase (PKA) induces a ligand-independent internalization of EGFR. (uio.no)
  • However, inhibition of the PKA catalytic activity does not mimic these peptides, suggesting that the peptides are disrupting the interaction of AKAP(s) with proteins other than PKA. (embl-heidelberg.de)
  • cAMP-dependent PKA is an ubiquitous serine/theonine protein kinase present in a variety of. (biomol.com)
  • Neurofilament function depends on the state of phosphorylation of the numerous serine/threonine residues in these proteins. (jneurosci.org)
  • Previous studies have identified serine and threonine residues phosphorylated in PHF-τ and have shown that τ can be phosphorylated at several of these sites by proline-directed protein kinases and cyclic AMP-dependent protein kinase. (wiley.com)
  • Protein Kinase A (PKA) is a serine/threonine kinase, which exists as a tetrameric holoenzyme. (sigmaaldrich.com)
  • cAMP activates cAMP-dependent protein kinase (PKA), which can directly modulate effector proteins by phosphorylation at PKA-specific serine or threonine residues or can alter the levels of the effector proteins themselves by modulating gene expression. (jneurosci.org)
  • GSK-3β belongs to a family of GSK-3, a multifunctional serine/threonine kinase ( 12 ) that has been implicated in multiple biological processes including embryonic development, cell differentiation, apoptosis, and insulin response ( 13 , 14 ). (aacrjournals.org)
  • Several kinases including Akt can attenuate GSK-3β enzymatic activity by phosphorylating the NH 2 -terminal serine, Ser 9 ( 13 ). (aacrjournals.org)
  • Syt12 is phosphorylated by cAMP-dependent protein kinase-A at serine-97 in an activity-dependent manner, suggesting a function for Syt12 in cAMP-dependent synaptic plasticity. (pubmedcentralcanada.ca)
  • Thus, Syt12 is essential for cAMP-dependent presynaptic LTP at mossy-fiber synapses, and a single amino-acid substitution that blocks the cAMP-dependent phosphorylation of Syt12 is sufficient to impair the function of Syt12 in mossy-fiber LTP, suggesting that cAMP-dependent phosphorylation of Syt12 on serine-97 contributes to the induction of mossy-fiber LTP. (pubmedcentralcanada.ca)
  • The regulation of the levels of cAMP-dependent protein kinases during myogenesis in L6 was studied, as these enzymes have been proposed to be involved in the control of this process. (uwo.ca)
  • Studies on the regulation of the catalytic subunit (C) of cAMP-dependent protein kinase showed that the increase in this subunit, which also occurs during myogenesis, is probably regulated at the level of transcription, and not by a change in its rate of degradation. (uwo.ca)
  • The results are consistent with the hypothesis that protein phosphorylation may play a role in the regulation of insulin secretion by Ca2+ and cyclic AMP. (biochemj.org)
  • Thus, the activity of the rat kidney pyruvate kinase type L is with all probability regulated by a reversible phosphorylation-dephosphorylation reaction, thereby indicating that hormonal regulation of gluconeogenesis via cyclic AMP may be of importance in the renal cortex. (elsevier.com)
  • Protein kinase A (PKA), the main effector of cAMP in eukaryotes, is a paradigm for the mechanisms of ligand-dependent and allosteric regulation in signalling. (nature.com)
  • 7500 members that confers ligand-dependent allosteric regulation to a diverse range of proteins 2 . (nature.com)
  • EC 2.7.1.37), and (b) hemin prevents this conversion by blocking the interaction of cyclic AMP with the kinase's regulation subunit, thereby rendering the enzyme inactive. (ias.ac.in)
  • Molecular cloning, cDNA structure, and regulation of the regulatory subunit of type II cAMP-dependent protein kinase from rat ovarian granulosa cells. (springer.com)
  • Hormonal regulation of cyclic AMP binding to specific receptor proteins in rat ovarian follicles. (springer.com)
  • The results demonstrated that GPER protein down-regulation significantly correlated with GPER promoter hypermethylation (p (cancerindex.org)
  • It has, however, never been clarified how regulation of Ca(2+)-dependent exocytosis by cAMP differs depending on the involvement of PKA and Epac, and depending on two types of secretory vesicles, large dense-core vesicles (LVs) and small vesicles (SVs). (biomedsearch.com)
  • GSK-3β is also involved in cell cycle regulation through the phosphorylation of cyclin D1, which results in the rapid proteolytic turnover of cyclin D1 protein ( 21 ). (aacrjournals.org)
  • Bansal G, Druey KM, Xie Z. R4 RGS proteins: regulation of G-protein signaling and beyond . (nih.gov)
  • Stathmin is a highly conserved 17 kDa protein that is crucial for the regulation of the cell cytoskeleton. (wikipedia.org)
  • Regulation of stathmin is cell cycle dependent and controlled by the cell's protein kinases in response to specific cell signals. (wikipedia.org)
  • Surprisingly, the primary downstream target of the adenylyl cyclase, the PKA, is not involved in the regulation of fusarubins, suggesting that additional, yet unidentified, cAMP-binding protein(s) exist. (nih.gov)
  • beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein. (duke.edu)
  • Thus, these studies demonstrate a novel, rapid regulation of SS RBC adhesion by a cAMP-dependent pathway and suggest that components of this pathway, particularly PKA, the β2-adrenergic receptor, and BCAM/Lu, should be further explored as potential therapeutic targets to inhibit SS RBC adhesion. (bloodjournal.org)
  • Although many studies have focused on Ca 2+ /calmodulin-dependent protein kinase II (CaMKII)-mediated regulation of spine dynamics and synaptic function in adult brain, much less is know about protein kinase A (PKA)-dependent regulation of spine shape dynamics during postnatal brain development. (pubmedcentralcanada.ca)
  • Our results identify synaptopodin as a substrate of PKA in hippocampal neurons and point to an essential role for synaptopodin in activity-dependent regulation of dendritic spine dynamics and synaptic plasticity in postnatal brain development. (pubmedcentralcanada.ca)
  • Down-regulation of Prkar1a by up to 70% was achieved in transgenic mouse tissues and embryonic fibroblasts, with concomitant changes in kinase activity and increased cell proliferation, respectively. (aacrjournals.org)
  • Reversible protein phosphorylation is one of the most important mechanisms for regulation and coordination of cellular processes, and is uttered through the actions of protein kinases and phosphatases. (uio.no)
  • There is a possibility that this protein is a physiological substrate of cyclic AMP-dependent protein kinase and that the phosphorylation and dephosphorylation of this substrate may be important in the translation control of adrenal polyadenylated messenger RNA. (sciencemag.org)
  • The occurrence of endogenous substrate proteins for Ca2+-dependent protein kinase, augmented by either phospholipid or calmodulin, and for cyclic AMP-dependent protein kinase was examined in homogenates and subcellular fractions of mouse pancreatic islets. (biochemj.org)
  • Examination of cyclic AMP-dependent protein phosphorylation revealed five substrate proteins, of Mr 23000-72000, present in the 27000g supernatant of mouse islets. (biochemj.org)
  • Vulliet, PR , Langan, TA & Weiner, N 1980, ' Tyrosine hydroxylase: A substrate of cyclic AMP-dependent protein kinase ', Proceedings of the National Academy of Sciences of the United States of America , vol. 77, no. 1, pp. 92-96. (elsevier.com)
  • VASP is a prominent substrate for both cGMP-dependent protein kinase (cGK) and cAMP-dependent protein kinase. (jimmunol.org)
  • Two C-terminal cyclic nucleotide binding (CNB) domains cooperatively bind two molecules of cAMP, resulting in a conformational change of the R subunit that releases the active catalytic kinase subunit(s) from the inhibitory pseudo-substrate or substrate site of PKAR. (nature.com)
  • Phosphorylation of substrate proteins leads to control of enzyme activity and/or gene expression. (yu.edu)
  • However, a difference in the processing efficiency of Ras switch I in the context of Ras, RalA, or RhoA indicates that protein regions outside Ras switch I also contribute to efficient RRSP substrate recognition. (bireme.br)
  • In conclusion, this work demonstrates that the GTPase recognition of RRSP is independent of the nucleotide state and is mainly driven by the Ras and Rap1 switch I loop and also influenced by additional protein-protein interactions, increasing the substrate specificity of RRSP. (bireme.br)
  • In transgenic mice expressing a surrogate substrate (GFPdgn), PKG activation by sildenafil increased myocardial proteasome activities and significantly decreased myocardial GFPdgn protein levels. (ahajournals.org)
  • UPS-mediated proteolysis generally involves 2 steps: targeting of the substrate protein by ubiquitination and the subsequent degradation of the ubiquitinated protein by the 26S proteasome. (ahajournals.org)
  • The C binding proteins will target the C subunit in close proximity to its substrate, and in this way increase the level of specificity in the cAMP-PKA signalling pathway. (uio.no)
  • Signaling governed by G proteins and cAMP is crucial for growth, secondary metabolism and sexual development in Fusarium fujikuroi. (nih.gov)
  • Mitochondrial metabolism requires inward and outward flux of hydrophilic metabolites, including ATP, ADP and respiratory substrates, through voltage-dependent anion channels (VDACs) in the mitochondrial outer membrane. (aspetjournals.org)
  • In conclusion, tubulin-dependent closure of VDACs represents a new mechanism contributing to the suppression of mitochondrial metabolism in the Warburg phenomenon. (aspetjournals.org)
  • VASP is believed to play an important role in controlling the cytoskeletal organization because it binds filamentous actin (F-actin) and profilin, a protein that forms complexes with G-actin and regulates actin dynamics ( 2 , 11 , 12 ). (jimmunol.org)
  • cAMP-dependent protein kinase (PKA) regulates numerous cellular processes in eukaryotes in response to extracellular and intracellular signals, via a flexible post-translational modification system. (yu.edu)
  • It has been reported that cAMP regulates Ca(2+)-dependent exocytosis via protein kinase A (PKA) and exchange proteins directly activated by cAMP (Epac) in neurons and secretory cells. (biomedsearch.com)
  • We conclude that protein kinase A-dependent phosphorylation of the D 1 receptor on Thr268 regulates a late step in the sorting of the receptor to the perinuclear region of the cell, but that phosphorylation of Thr268 is not required for receptor sequestration or maximal desensitization of cyclic AMP accumulation. (aspetjournals.org)
  • The ubiquitin-proteasome system (UPS) mediates the degradation of most intracellular proteins and regulates diverse cellular processes, including protein quality control. (ahajournals.org)
  • Epistasis analysis supports a model in which the Gpr1 receptor regulates pseudohyphal growth via the Gpa2p-cAMP-PKA pathway and independently of both the MAP kinase cascade and the PKA related kinase Sch9. (genetics.org)
  • In summary, our studies reveal a novel G protein-coupled receptor senses nutrients and regulates the dimorphic transition to filamentous growth via a Gα protein-cAMP-PKA signal transduction cascade. (genetics.org)
  • Previous physiological and pharmacological experiments have demonstrated that the Chlamydomonas flagellar axoneme contains a cAMP-dependent protein kinase (PKA) that regulates axonemal motility and dynein activity. (embl-heidelberg.de)
  • The R 2 homodimer is formed by an N-terminal dimerization/docking (DD) domain that also mediates sub-cellular localization via A kinase anchoring proteins (AKAPs). (nature.com)
  • The cAMP-dependent protein kinase (PKA) is targeted to specific subcellular compartments through its interaction with A-kinase anchoring proteins (AKAPs). (embl-heidelberg.de)
  • These data suggest that sperm contains several proteins that bind to AKAPs in a manner similar to RII and imply that AKAPs may have additional and perhaps unique functions in spermatozoa. (embl-heidelberg.de)
  • cAMP-dependent protein kinase is targeted to discrete subcellular locations by a family of specific anchor proteins (A-kinase anchor proteins, AKAPs). (embl-heidelberg.de)
  • Although AKAPs have been identified on the basis of their interaction with PKA, they also bind other signaling molecules, mainly phosphatases and kinases, that regulate AKAP targeting and activate other signal transduction pathways.We suggest that AKAP forms a "transduceosome" by acting as an autonomous multivalent scaffold that assembles and integrates signals derived from multiple pathways. (embl-heidelberg.de)
  • This membrane-anchoring myristylation switch is independent of A Kinase Anchoring Proteins (AKAPs) that target PKA to membranes by other mechanisms. (rcsb.org)
  • How specificity is maintained in the cAMP-PKA pathway is not fully understood but involves targeting of the R subunit dimer to subcellular structures through proteins that are termed A kinase anchoring proteins (AKAPs). (uio.no)
  • After incubation of the phosphorylated kidney pyruvate kinase with phosphatase the phosphoenolpyruvate saturation curve was found to be identical to that for the unphosphorylated enzyme. (elsevier.com)
  • The inactivation is stable during purification in the presence of protein phosphatase inhibitors, and is associated with a 30-40% increase in the labelling of enzyme isolated from 32P-labelled cells. (duke.edu)
  • [8] [9] Then, the NAD(P)H dependent enzyme dihydropteridine reductase (DHPR) converts q-BH 2 back to BH 4 . (wikipedia.org)
  • When AVP binds with V2R , via the G-protein , it stimulates the enzyme , adenylyl cyclase (AdC). (ndif.org)
  • Reaction of the catalytic subunit with antiserum and separation of the immunoglobulin G fraction by Protein A-Sepharose quantitatively removed the enzyme from solutions. (biochemj.org)
  • Cyclic AMP-dependent protein kinase purified from the particulate fraction of bovine heart reacted with the antiserum to the same degree as the soluble enzyme, whereas two cyclic AMP-independent kinases separated from the particle fraction neither reacted with the antiserum nor influenced the reaction of the antibodies with the cyclic AMP-dependent protein kinase. (biochemj.org)
  • Immunotitration of the protein kinase catalytic subunit C from rat liver revealed that the antibodies had rather similar reactivities towards the rat liver and the bovine heart enzyme. (biochemj.org)
  • At the postsynaptic membrane of neuronal excitatory synapses, postsynaptic density-95, discs large, zona occludens-1 (PDZ) * domain-containing scaffold proteins combine with cytoskeletal proteins in the postsynaptic density (PSD) ( Sheng and Scala, 2001 ). (rupress.org)
  • Calcium-calmodulin-dependent kinase II modulates Kv4.2 channel expression and upregulates neuronal A-type potassium currents. (bcm.edu)
  • At the neuronal level, cells with higher levels of excitability (for example lower slow afterhyperpolarization) are more likely to be recruited into a memory trace, and substantial evidence exists implicating the cellular transcription factor CREB (cyclic AMP responsive element-binding protein) in this process. (wikipedia.org)
  • The transcription factor cAMP response element-binding protein (CREB) is a well-studied mechanism of neuronal memory allocation. (wikipedia.org)
  • Williams, John A. / Adenylyl cyclase 6 mediates the action of cyclic AMP-dependent secretagogues in mouse pancreatic exocrine cells via protein kinase A pathway activation . (elsevier.com)
  • While Gα olf expression was not detectable in β-cells, experiments using inhibitors and siRNA revealed that the pathway dependent on phospholipase C-inositol triphosphate, rather than cAMP-protein kinase A, mediates GSIS potentiation via OLFR15. (nature.com)
  • Together with the effects of isobutylmethylxanthine and the stimulatory cyclic AMP analogues, this strongly indicates that cyclic-AMP-dependent protein kinase is an essential component of the signal transduction pathway, although clearly it does not directly phosphorylate acetyl-CoA carboxylase. (duke.edu)
  • We believe these findings to be related to the well-established role of cyclic AMP in signal transduction. (unibas.ch)
  • Scaffold, anchoring, and adaptor proteins coordinate the assembly and localization of signaling complexes providing efficiency and specificity in signal transduction. (rupress.org)
  • Recent research advances have shown that molecular associations regulating subcellular targeting of signaling proteins are crucial for efficient and specific signal transduction ( Smith and Scott, 2002 ). (rupress.org)
  • These include the structural basis for specificity of their binding interactions and ideas about how they organize both small local protein complexes used for signal transduction (transducisomes) and larger two-dimensional complexes like cell junctions and plasma membrane domains. (jci.org)
  • Some evidence has suggested that APSES proteins function in the cAMP signaling pathways. (genetics.org)
  • Moreover, we found that taurine enhanced K(V) channels via intracellular protein kinase C-mediated pathways. (biomedsearch.com)
  • Two cAMP-dependent pathways differentially regulate exocytosis of large dense-core and small vesicles in mouse beta-cells. (biomedsearch.com)
  • Multifunctional β-catenin, with critical roles in both cell-cell adhesion and Wnt-signaling pathways, was among HeLa cell proteins coimmunoprecipitated by antibodies against brefeldin A-inhibited guanine nucleotide-exchange factors 1 and 2 (BIG1 or BIG2) that activate ADP-ribosylation factors (Arfs) by accelerating the replacement of bound GDP with GTP. (pnas.org)
  • Stathmin is a ubiquitous phosphorylated protein which makes it act as an intracellular relay for diverse regulatory pathways, functioning through a variety of second messengers. (wikipedia.org)
  • VASP is phosphorylated by cAMP-dependent protein kinase (cAK) or cGMP-dependent protein kinase (cGK). (jimmunol.org)
  • Cyclic AMP-dependent protein kinase (PKA) and cGMP-dependent protein kinase (PKG) are autoinhibited through multiple interactions between their respective regulatory and catalytic domains. (bioscience.org)
  • Unfortunately, the biochemical precipitation techniques used to study the assembly of protein complexes are limited by steric hindrance from bulky fusion proteins or antibodies, use of detergents to solubilize complexes from cells, and poor yield of proteins precipitating through intermediate proteins. (rupress.org)
  • Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. (duke.edu)
  • Titration with the antibodies also revealed the presence of a cyclic AMP-independent histone kinase in bovine heart protein kinase I preparations obtained by DEAE-cellulose chromatography. (biochemj.org)
  • Search, Find and Buy Antibodies, ELISA Kits and Proteins. (antibodies-online.com)
  • On www.antibodies-online.com are 49 Protein Kinase C, delta (PKCd) ELISA Kits from 10 different suppliers available. (antibodies-online.com)
  • Additionally we are shipping PKC delta Antibodies (296) and PKC delta Proteins (25) and many more products for this protein. (antibodies-online.com)
  • We now show that hemin blocks cyclic AMP binding because it itself binds specifically to the regulatory subunit. (ias.ac.in)
  • The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. (cancerindex.org)
  • However, when the cyclic-AMP binds to the regulatory subunit, the catalytic subunit is released and can then catalyze the transfer of phosphate from ATP to various proteins. (sigmaaldrich.com)
  • These domains are, figuratively speaking, the glue that binds protein complexes together, and their unique specificity and regulated binding determine the distinct compositions of different complexes. (jci.org)
  • Treatment of adipocytes with various agents which activate cyclic-AMP-dependent protein kinase by receptor-independent mechanisms (forskolin, cyclic AMP analogues, isobutylmethylxanthine) also produced inactivation of acetyl-CoA carboxylase and increased phosphorylation at Ser-79. (duke.edu)
  • These kinases are related to the mammalian kinases that activate AMPK by phosphorylating the equivalent T-loop threonine (Thr172) (reviewed in references 17 and 18 ). (asm.org)
  • It is not entirely clear why S. cerevisiae has three different kinases that can activate Snf1. (asm.org)
  • Home Research Outputs Expression of cyclic AMP-dependent protein KINASE-I isoforms. (lu.se)
  • The glycine-rich N-terminal domain unique to GSK-3α and the conserved kinase domain shared by both isoforms are highlighted. (biologists.org)
  • Laminin is an extracellular matrix (ECM) protein distributed throughout most vascular beds, 1 with isoforms 10 and 11 specifically supporting more SS RBC adhesion than other ECM proteins tested under physiologic shear and flow conditions. (bloodjournal.org)
  • Activation of Cdk2 Stimulates Proteasome-dependent Truncation of. (yasni.com)
  • Our results suggest that cAMP stimulates CREB-dependent gene transcription via a two-step process by inducing release of an excitatory amino acid, identified here as l -aspartate, which then activates NMDARs, leading to Ca 2+ entry and gene transcription. (jneurosci.org)
  • Lysophosphatidylcholines prime polymorphonuclear neutrophil through Hck (show HCK ELISA Kits )-dependent activation of PKCdelta , which stimulates PKCgamma (show PRKCG ELISA Kits ), resulting in translocation of phosphorylated p47(phox). (antibodies-online.com)
  • This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. (novusbio.com)
  • the major endogenous substrates in the homogenate were two proteins of Mr 53000 and 100000. (biochemj.org)
  • No common substrates for either the two Ca2+-dependent phosphorylation systems or for the cyclic AMP-dependent and the Ca2+-calmodulin-dependent phosphorylation systems were noted. (biochemj.org)
  • On the other hand, the actions of the cyclic AMP-sensitive and the Ca2+-phospholipid-sensitive systems may be overlapping, since two common substrates for them were noted in the 27000g-supernatant fraction. (biochemj.org)
  • Another peculiarity compared with other protein kinases is its preference for primed substrates, that is, substrates previously phosphorylated by another kinase. (biologists.org)
  • The beta-adrenergic receptor kinase (beta ARK) phosphorylates its membrane-associated receptor substrates, such as the beta-adrenergic receptor, triggering events leading to receptor desensitization. (duke.edu)
  • 1976) "Synthetic Hexapeptide Substrates and Inhibitors of 3':5'-Cyclic AMP-Dependent Protein Kinase" Proc. (patentgenius.com)
  • 1990) "Identification of Major Nucleolar Proteins As Candidate Mitotic Substrates of cdc2 Kinase" Cell 60:791-801. (patentgenius.com)
  • In the method of the invention, a protein kinase is contacted with an oriented degenerate peptide library, peptides within the library which are substrates for the kinase are converted to phosphopeptides and the phosphopeptides are separated from non-phosphorylated peptides. (patentgenius.com)
  • The invention also provides peptide substrates for protein kinase A, cell cycle control kinases, src family kinases, the EGF receptor and p92.sup.c-fps/fes based upon amino acid sequence motifs for the phosphorylation sites of these kinases. (patentgenius.com)
  • Isolation and characterization of a protein from rat testis which inhibits cyclic AMP-dependent protein kinase and phosdiesterase. (duke.edu)
  • Ultrasound-targeted microbubble destruction of calcium channel subunit α 1D siRNA inhibits breast cancer via G protein-coupled receptor 30. (cancerindex.org)
  • Protein Kinase A (PKA) inhibits hormone-sensitive lipase translocation from cytosol to storage droplets and blocks lipolysis. (sigmaaldrich.com)
  • The subcellular distribution of the regulatory (RII) subunit of cyclic AMP-dependent protein kinase (PK-A) was analyzed at the electron-microscopical level using thawed cryo-sections of Madin Darby Bovine Kidney (MDBK) cells. (unibas.ch)
  • Schematic diagram of a vertebrate polarized epithelial cell, showing the distinct subcellular localization of several epithelial PDZ proteins into the apical, basal-lateral, and junctional domains. (jci.org)
  • Cellular localization and age-dependent changes in mRNA for cyclic adensoine 3',5'-monophosphate-dependent protein kinases in rat testis. (springer.com)
  • Finally, we demonstrated AKAP79-regulated membrane localization of the MAGUK synapse-associated protein 97 (SAP97), suggesting that AKAP79 functions to organize even larger signaling complexes. (rupress.org)
  • β2-Adrenergic Receptor Lacking the Cyclic AMP-Dependent Protein Kinase Consensus Sites Fully Activates Extracellular Signal-Regulated Kinase 1/2 in Human Embryonic Kidney 293 Cells: Lack of Evidence for Gs/Gi Switching. (aspetjournals.org)
  • Stimulation of the β 2 -adrenergic receptor (β 2 AR) in human embryonic kidney (HEK) 293 cells causes a transient activation of Extracellular Signal-Regulated Kinase (ERK) 1/2. (aspetjournals.org)
  • cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes from yeast to men. (nih.gov)
  • Extracellular cyclic AMP-dependent protein kinase A (ECPKA) has been determined to increase in the serum of cancer-bearing dogs. (scicombinator.com)
  • Expression in Escherichia coli of BCY1, the regulatory subunit of cyclic AMP-dependent protein kinase from Saccharomyces cerevisiae. (cshl.edu)
  • Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. (yeastgenome.org)
  • In Saccharomyces cerevisiae , Snf1 protein kinase is important for growth on carbon sources that are less preferred than glucose. (asm.org)
  • Measurement of the mRNA levels for the type I regulatory subunit (R{dollar}\sb{lcub}\rm I{rcub}{dollar}) of cAMP-dependent protein kinase, and studies on the rates of transcription from the gene coding for this protein, showed that the increase in R{dollar}\sb{lcub}\rm I{rcub}{dollar} which occurs during myogenesis is is not regulated transcriptionally. (uwo.ca)
  • The PKA1 gene encoding the major cyclic AMP (cAMP)-dependent protein kinase catalytic subunit was identified and disrupted. (asm.org)
  • The PKR1 gene encoding the protein kinase A (PKA) regulatory subunit was also identified and disrupted. (asm.org)
  • Investigating the factors participating in SF-1 action, we found that c-Jun and homeodomain-interacting protein kinase 3 (HIPK3) were required for basal and cAMP-stimulated expression of one major steroidogenic gene, CYP11A1. (nih.gov)
  • Somatic mutations of the catalytic subunit of the cyclic AMP-dependent protein kinase (PRKACA) gene have recently been identified in about 35% of cortisol-producing adenomas (CPAs), with the affected patients showing overt Cushing's syndrome. (cdc.gov)
  • The regulatory subunit of neural cAMPdependent protein kinase II represents a unique gene product. (springer.com)
  • This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • Although highly homologous within their kinase domains (98% identity), the two gene products share only 36% identity in the last 76 C-terminal residues ( Woodgett, 1990 ). (biologists.org)
  • A gene on chromosome 5q35.1 that encodes G protein-coupled dopamine receptor D1, which is the most abundant dopamine receptor in the CNS. (thefreedictionary.com)
  • Stathmin, also known as metablastin and oncoprotein 18 is a protein that in humans is encoded by the STMN1 gene. (wikipedia.org)
  • In addition, we show here that cAMP repression applies to both Msn2/4p-dependent and -independent control of gene expression at the diauxic shift. (asm.org)
  • Major changes in the pattern of gene expression occurring during this transition have been characterized by analysis of the protein pattern obtained by two-dimensional (2-D) gel electrophoresis. (asm.org)
  • The second activity peak was stimulated by nanomolar concentrations of cyclic AMP. (elsevier.com)
  • The reactivity of the holoenzymes with the antiserum was identical in the absence or the presence of dissociating concentrations of cyclic AMP. (biochemj.org)
  • These results suggest that both protein kinase C and tyrosine kinase are involved in melanin production by cyclic AMP-dependent pathway and NFkB pathway may play an important role in cyclic AMP-dependent melanin production in B16 melanoma cells. (koreascience.or.kr)
  • Burke, T. R. (1992) "Protein-Tyrosine Kinase Inhibitors" Drugs Fut. (patentgenius.com)
  • Blass, Kronfeld, Kazimirsky, Blumberg, Brodie: Tyrosine phosphorylation of protein kinase Cdelta is essential for its apoptotic effect in response to etoposide. (antibodies-online.com)
  • Anesthesia-resistant memory (ARM) is deficient in the memory mutant radish , whereas long-lasting protein-synthesis-dependent memory is eliminated by overexpressing the blocking form of the transcription factor cAMP-response element-binding protein or by feeding cycloheximide ( 4 , 5 , 6 ). (pnas.org)
  • cAMP-response element-binding protein is involved in long-term memory in Drosophila , Aplysia , and mice ( 6 , 8 , 9 ). (pnas.org)
  • cAMP can stimulate the transcription of many activity-dependent genes via activation of the transcription factor, cAMP response element-binding protein (CREB). (jneurosci.org)
  • We have analyzed the importance for hyphal morphogenesis of a putative phosphorylation site for protein kinase A (PKA), threonine-206, within an Efg1p domain highly conserved among APSES proteins. (genetics.org)
  • Catalytic activation of Snf1 occurs through phosphorylation of its conserved T-loop threonine (Thr210) ( 12 ) by upstream kinases ( 40 , 62 ). (asm.org)
  • These accumulated findings provide insights into the mechanisms by which these enzymes produce their specific physiological effects and are helpful in considering the actions of other protein kinases as well. (nih.gov)
  • Molecular mechanisms controlling the localisation of protein kinase A. (nih.gov)
  • Signaling mechanisms implicated in these metaplastic effects include autophosphorylation of αCaMKII, changes in NMDA receptor subunit composition, and activation of voltage-dependent calcium channels. (wikipedia.org)
  • BIG proteins also contain A-kinase anchoring protein (AKAP) sequences that can act as scaffolds for multimolecular assemblies that facilitate and limit cAMP signaling temporally and spatially. (pnas.org)
  • Flagellar radial spoke protein 3 is an A-kinase anchoring protein (AKAP). (embl-heidelberg.de)
  • Here we test the hypothesis that the axoneme contains an A-kinase anchoring protein (AKAP). (embl-heidelberg.de)
  • cAMP activates protein kinase A (PKA). (ndif.org)
  • An endogenous polysomal cyclic AMP-dependent protein kinase specifically phosphorylates a 150,000-dalton peptide bound to an adrenocortical polyadenylated messenger ribonucleoprotein complex. (sciencemag.org)
  • Most phosphorylation occurs in the lys-ser-pro (KSP) repeats in the C-terminal tail domains of NF-H and NF-M. In our previous study, cyclin-dependent kinase 5 (cdk5) was shown to phosphorylate specifically the KSPXK repeats in rat NF-H. Because 80% of the repeats are of the KSPXXXK type, it was of interest to determine which kinase phosphorylates these motifs. (jneurosci.org)
  • The 'regulatory' component had specific binding for cyclic AMP, and it inhibited phosphotransferase activity of the homologous 'catalytic component' or of the 'catalytic subunit' from bovine heart. (elsevier.com)
  • Isolation and sequence of a tryptic peptide containing the autophosphorylation site of the regulatory subunit of bovine brain protein kinase II. (springer.com)
  • Protein Kinase A from bovine heart has been used to determine its activity using a molecular beacon probe. (sigmaaldrich.com)
  • Protein Kinase A from bovine heart has been used to inhibit the phosphorylation of myosin. (sigmaaldrich.com)
  • An antiserum against the catalytic subunit C of cyclic AMP-dependent protein kinase, isolated from bovine heart type II protein kinase, was produced in rabbits. (biochemj.org)
  • Cyclic AMP-dependent protein kinase activity was further purified by gel filtration, affinity chromatography on histone-agarose and cyclic AMP-agarose, as well as by chromatography on CM-Sephadex. (elsevier.com)
  • These protein complexes mediate the adhesive properties of particular cells, the formation of the paracellular barrier (tight junctions), ion transport, and transmission of signals between adjacent cells that regulate growth, differentiation, and homeostasis. (jci.org)
  • and analogue, where local gradients of cAMP concentration regulate cAMP effectors more remote from AC.Binary signaling requires localized delivery of cAMP, whereas analogue signaling is more dependent on localized cAMP degradation. (nih.gov)
  • When activated by the second messengers such as cAMP and calcium ions, enzymes such as PKA and MAP kinase can translocate to the nucleus and phosphorylate CREB to initiate transcription of target genes. (wikipedia.org)
  • Three protein kinases-Sak1, Tos3, and Elm1-have been identified that can phosphorylate Thr210 of Snf1 ( 22 , 41 , 55 ). (asm.org)
  • It encodes a protein with 23 predicted cyclic-AMP-dependent protein kinase (PKA) phosphorylation sequences. (pnas.org)
  • These 80-90 amino acids sequences have now been identified in well over 75 proteins and are characteristically expressed in multiple copies within a single protein. (jci.org)
  • An enhancer element and a functional cyclic AMP-dependent protein kinase are required for expression of adrenocortical 21-hydroxylase. (harvard.edu)
  • The expression and phosphorylation state of the vasodilator-stimulated phosphoprotein (VASP), a membrane-associated focal adhesion protein, was investigated in human neutrophils. (jimmunol.org)
  • Oligonucleotide-directed mutagenesis was used to create an NdeI restriction site at the natural ATG of the yeast R. This facilitated construction of the T7 expression vector so that the sequence of the protein produced was identical to the natural R subunit. (cshl.edu)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Recently, we found that G-protein-coupled estrogen receptor (GPER) protein expression decreased during breast carcinogenesis, and that GPER promoter is methylated. (cancerindex.org)
  • Studies using animal experimental models have suggested that the beta2-adrenoceptor is uncoupled in association with alterations in the expression of G-protein-coupled receptor kinases (GRK) 2/3 in heart failure. (curehunter.com)
  • In both models we observed a significant reduction of EGFR expression at the protein but not mRNA level. (uio.no)
  • We further show that synaptopodin is required for full expression of PKA-dependent hippocampal long-term potentiation in 15- and 21-d-old, but not adult, mice. (pubmedcentralcanada.ca)
  • Dynamin-related protein 1 (Drp1), a critical mitochondrial fission protein, translocated to mitochondria early during tubular cell injury, and both siRNA knockdown of Drp1 and expression of a dominant-negative Drp1 attenuated mitochondrial fragmentation, cytochrome c release, caspase activation, and apoptosis. (jci.org)
  • Activation of PKG increased proteasome peptidase activities, facilitated proteasome-mediated degradation of surrogate (enhanced green fluorescence protein modified by carboxyl fusion of degron CL1) and bona fide (CryAB R120G ) misfolded proteins, and attenuated CryAB R120G overexpression-induced accumulation of ubiquitinated proteins and cellular injury. (ahajournals.org)
  • In neurodegenerative diseases, normal cellular waste disposal systems fail to clear misfolded proteins. (alzforum.org)
  • Ser 656 is phosphorylated by cyclic AMP‐dependent protein kinase and dephosphorylated by calcineurin, and its phosphorylation state is controlled by sympathetic tone, calcium levels and cell viability. (embopress.org)
  • AC6 appeared to be an important isoform because protein kinase A (PKA), PKC and calcium all inhibited VIP-induced cAMP formation, whereas calcineurin or calmodulin did not modify the response to VIP. (elsevier.com)
  • Here we show that this process is dependent on cAMP-dependent protein kinase A (PKA) activity and regulated by the high-osmolarity glycerol response (HOG) MAPKs SakA and MpkC. (asm.org)
  • To examine these phenomena, we characterized agonist activation of ERK1/2 in HEK293 cells by the endogenous β 2 AR and in HEK293 cells stably overexpressing either the wild-type β 2 AR or a substitution mutant β 2 AR (PKA − ) that lacks the cyclic AMP-dependent protein kinase (PKA) consensus phosphorylation sites (S261A, S262A and S345A, S346A). (aspetjournals.org)
  • Mechanism of protein binding and the basis for specificity. (jci.org)
  • Cyclic-AMP Receptor Proteins in Heart Muscle of Rats Flown on Cosmos 1887. (nasa.gov)
  • To investigate the effect of protein kinase on melanin production via cAMP-dependent pathway, we measured the melanin amount and tyrosinase activity in B16 melanoma cells stimulated by alpha-melanocyte stimulating hormone (MSH), forskolin and 8-Br-cAMP. (koreascience.or.kr)
  • Calcium-dependent exocytosis of SVs was potentiated by the cAMP-elevating agent forskolin, and the potentiating effect was unaffected by antagonists of PKA and was mimicked by the Epac-selective agonist 8-(4-chlorophenylthio)-2'-O-methyl cAMP, unlike that on LVs. (biomedsearch.com)
  • Forskolin or the stress mediator epinephrine further elevates cAMP in SS RBCs and increases adhesion of SS RBCs to laminin in a protein kinase A (PKA)-dependent manner, with the low-density population being the most responsive. (bloodjournal.org)
  • Long-term memory in Drosophila is separable into two components: consolidated, anesthesia-resistant memory and long-lasting, protein-synthesis-dependent memory. (pnas.org)
  • i ) resistance to treatments such as anesthesia or electroconvulsive shock that disrupt the patterned activity of the brain ( 1 ), and ( ii ) requirement for new protein synthesis ( 2 , 3 ). (pnas.org)
  • ARM and long-lasting protein-synthesis-dependent memory may interact at times, at least in anatomically mutant flies ( 7 ). (pnas.org)
  • The specific defect of the radish mutant, diminished ARM, normal long-lasting protein-synthesis-dependent memory, provides a key to obtaining mechanistic information about ARM. (pnas.org)
  • We analyzed the two-dimensional gel electrophoresis pattern of protein synthesis of the msn2 msn4 double mutant and compared it with that of the wild-type strain during exponential growth phase and at the diauxic transition. (asm.org)
  • Zhang, Keane, Zhu, Sharma, Rozengurt, Strieter, Dubinett, Huang: Interleukin-7 and transforming growth factor-beta play counter-regulatory roles in protein kinase C-delta-dependent control of fibroblast collagen synthesis in pulmonary fibrosis. (antibodies-online.com)
  • eEF1A1 is associated with ribosome activity and protein synthesis and brings the incoming aminoacyl-tRNA in a GTP-dependent manner to a site within the ribosome referred to as the aminoacyl (A) site. (uio.no)
  • Our data implies that we have identified a novel binding partner for the C subunit of PKA expressed in all tissues except differentiated skeletal muscle, linking the regulatory role of the cAMP-PKA signalling pathway to protein synthesis. (uio.no)