Cumulus Cells
Oocytes
Ovarian Follicle
An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS.
Oogenesis
Fertilization in Vitro
Bone Morphogenetic Protein 15
Growth Differentiation Factor 9
Granulosa Cells
Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH).
In Vitro Oocyte Maturation Techniques
Meiosis
Follicular Fluid
Sperm-Ovum Interactions
Cloning, Organism
Blastocyst
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
Ovary
The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.
Hyaluronoglucosaminidase
Follicle Stimulating Hormone
A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Follicle-stimulating hormone stimulates GAMETOGENESIS and the supporting cells such as the ovarian GRANULOSA CELLS, the testicular SERTOLI CELLS, and LEYDIG CELLS. FSH consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity.
Embryo Culture Techniques
Phosphofructokinase-1, Type C
An allosteric enzyme that regulates glycolysis by catalyzing the transfer of a phosphate group from ATP to fructose-6-phosphate to yield fructose-1,6-bisphosphate. In the humans, 6-phosphofructose-1-kinase isozyme C is found in platelets, brain, heart, kidney, colon and testis. This isozyme C can exist as the homotetramer of C subunits (P subunits), or heterotetramer of C type and L type subunits.
Chorionic Gonadotropin
A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN).
Cattle
Zona Pellucida
Cells, Cultured
TGF-beta Superfamily Proteins
Sperm Injections, Intracytoplasmic
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Fertilization
Nuclear Transfer Techniques
Embryo Transfer
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
Superovulation
Gonadotropins, Equine
Gonadotropins secreted by the pituitary or the placenta in horses. This term generally refers to the gonadotropins found in the pregnant mare serum, a rich source of equine CHORIONIC GONADOTROPIN; LUTEINIZING HORMONE; and FOLLICLE STIMULATING HORMONE. Unlike that in humans, the equine LUTEINIZING HORMONE, BETA SUBUNIT is identical to the equine choronic gonadotropin, beta. Equine gonadotropins prepared from pregnant mare serum are used in reproductive studies.
Cleavage Stage, Ovum
The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.
Spermatozoa
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
Progesterone
The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.
Luteinizing Hormone
A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Luteinizing hormone regulates steroid production by the interstitial cells of the TESTIS and the OVARY. The preovulatory LUTEINIZING HORMONE surge in females induces OVULATION, and subsequent LUTEINIZATION of the follicle. LUTEINIZING HORMONE consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH and FSH), but the beta subunit is unique and confers its biological specificity.
Hyaluronic Acid
Oocyte Retrieval
Heptanol
Cysteamine
A mercaptoethylamine compound that is endogenously derived from the COENZYME A degradative pathway. The fact that cysteamine is readily transported into LYSOSOMES where it reacts with CYSTINE to form cysteine-cysteamine disulfide and CYSTEINE has led to its use in CYSTINE DEPLETING AGENTS for the treatment of CYSTINOSIS.
Connexin 43
Gonadotropins
Hormones that stimulate gonadal functions such as GAMETOGENESIS and sex steroid hormone production in the OVARY and the TESTIS. Major gonadotropins are glycoproteins produced primarily by the adenohypophysis (GONADOTROPINS, PITUITARY) and the placenta (CHORIONIC GONADOTROPIN). In some species, pituitary PROLACTIN and PLACENTAL LACTOGEN exert some luteotropic activities.
Cryoprotective Agents
Receptors, LH
Those protein complexes or molecular sites on the surfaces and cytoplasm of gonadal cells that bind luteinizing or chorionic gonadotropic hormones and thereby cause the gonadal cells to synthesize and secrete sex steroids. The hormone-receptor complex is internalized from the plasma membrane and initiates steroid synthesis.
Coculture Techniques
Pregnancy
Cell Communication
Buffaloes
Gap Junctions
Connections between cells which allow passage of small molecules and electric current. Gap junctions were first described anatomically as regions of close apposition between cells with a narrow (1-2 nm) gap between cell membranes. The variety in the properties of gap junctions is reflected in the number of CONNEXINS, the family of proteins which form the junctions.
Paracrine Communication
Embryo, Mammalian
Epidermal Growth Factor
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
Parthenogenesis
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Cryopreservation
Receptors, FSH
Mitogen-Activated Protein Kinase 14
A 38-kDa mitogen-activated protein kinase that is abundantly expressed in a broad variety of cell types. It is involved in the regulation of cellular stress responses as well as the control of proliferation and survival of many cell types. The kinase activity of the enzyme is inhibited by the pyridinyl-imidazole compound SB 203580.
Intercellular Signaling Peptides and Proteins
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
Ovulation Induction
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Metaphase
Ethylene Glycol
A colorless, odorless, viscous dihydroxy alcohol. It has a sweet taste, but is poisonous if ingested. Ethylene glycol is the most important glycol commercially available and is manufactured on a large scale in the United States. It is used as an antifreeze and coolant, in hydraulic fluids, and in the manufacture of low-freezing dynamites and resins.
Estradiol
Fallopian Tubes
A pair of highly specialized muscular canals extending from the UTERUS to its corresponding OVARY. They provide the means for OVUM collection, and the site for the final maturation of gametes and FERTILIZATION. The fallopian tube consists of an interstitium, an isthmus, an ampulla, an infundibulum, and fimbriae. Its wall consists of three histologic layers: serous, muscular, and an internal mucosal layer lined with both ciliated and secretory cells.
Morula
Follicular Phase
Vitrification
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Glucuronosyltransferase
Pregnancy Rate
Culture Media, Conditioned
Horses
Luteinization
Hypoxanthine
Gene Expression
Reverse Transcriptase Polymerase Chain Reaction
Follistatin
A broadly distributed protein that binds directly to ACTIVINS. It functions as an activin antagonist, inhibits FOLLICLE STIMULATING HORMONE secretion, regulates CELL DIFFERENTIATION, and plays an important role in embryogenesis. Follistatin is a single glycosylated polypeptide chain of approximately 37-kDa and is not a member of the inhibin family (INHIBINS). Follistatin also binds and neutralizes many members of the TRANSFORMING GROWTH FACTOR BETA family.
Follicular Atresia
Pyruvic Acid
Cystine
Infertility
Sperm Motility
Alpha-Globulins
Tissue Culture Techniques
Gene Expression Regulation, Developmental
Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat. (1/273)
During cryopreservation, the immature oocyte is subjected to anisosmotic conditions potentially impairing subsequent nuclear and cytoplasmic maturation in vitro. In preparation for cryopreservation protocols and to characterize osmotic tolerance, cat cumulus-oocyte complexes (COC) at the germinal vesicle (GV) stage were exposed for 15 min to sucrose solutions ranging from 100 to 2,000 mOsm and then examined for structural integrity and developmental competence in vitro. Osmolarities > or =200 and < or =750 mOsm had no effect on incidence of oocyte nuclear maturation, fertilization success, and blastocyst formation compared to control COC (exposed to 290 mOsm). This relatively high osmotic tolerance of the immature cat oocyte appeared to arise from a remarkable stability of the GV chromatin structure as well as plasticity in mitochondrial distribution, membrane integrity, and ability to maintain cumulus-oocyte communications. Osmolarities <200 mOsm only damaged cumulus cell membrane integrity, which contributed to poor nuclear maturation but ultimately had no adverse effect on blastocyst formation in vitro. Osmolarities >750 mOsm compromised nuclear maturation and blastocyst formation in vitro via disruption of cumulus-oocyte communications, an effect that could be mitigated through 1,500 mOsm by adding cytochalasin B to the hyperosmotic solutions. These results (1) demonstrate, for the first time, the expansive osmotic tolerance of the immature cat oocyte, (2) characterize the fundamental role of cumulus-oocyte communications when tolerance limits are exceeded, and (3) reveal an interesting hyperosmotic tolerance of the immature oocyte that can be increased two-fold by supplementation with cytochalasin B. (+info)Reduction of connexin 43 in human cumulus cells yields good embryo competence during ICSI. (2/273)
PURPOSE: The purpose of this study was to predict developmental competence of human oocytes during ICSI via analysis of connexin 43 (Cx43) in cumulus cells surrounding mature oocytes. MATERIALS AND METHODS: Human cumulus cells were manually separated from the oocyte-cumulus complex under a microscope. Cx43 mRNA was expressed by real-time quantitative polymerase chain reaction (RT-PCR) measurement in cumulus cells. RESULTS: There was no significant relationship between expression of Cx43 and fertilisation or cleavage rate. However, Cx43 expression was lower in the good morphology group (blastomeres>7 cells with fragmentation<10% on day 3) when compared to the other groups (p=0.035). CONCLUSIONS: These results suggest that full reduction of Cx43 expression on cumulus cells at the time of oocyte collection during ICSI is essential for developmental competence of human oocytes. (+info)Altered composition of the cumulus-oocyte complex matrix during in vitro maturation of oocytes. (3/273)
BACKGROUND: In vitro maturation (IVM) of mammalian oocytes has potential health benefits for patients undergoing assisted reproduction as an alternative to gonadotrophin treatment. This procedure is also useful for studying the process of oocyte and early embryo development. However, oocytes undergoing IVM have much lower competence than in vivo matured oocytes. Efforts to optimize IVM success have focused on replicating in vivo timing, hormonal milieu and cumulus cell responses associated with maturing oocytes. We have previously identified two extracellular matrix proteins, the protease Adamts1 and hyaluronan-binding proteoglycan Versican, produced by mural granulosa cells that selectively incorporate into the periovulatory cumulus-oocyte complex (COC). METHODS: Murine COC were cultured in the presence of epidermal growth factor and/or FSH. mRNA and protein were measured by real time PCR and Western blot and compared to in vivo derived COC. RESULTS: COCs from mice that underwent IVM for 6 or 20 h in the presence of epidermal growth factor, FSH or in combination had a > 10-fold reduction in mRNA (P < 0.05) for Adamts1 and Vcan when compared with in vivo matured COCs. Hyaluronan synthase 2 expression was up-regulated up to 8-fold (P < 0.05) over the unstimulated control, demonstrating successful induction of cumulus gene expression by the IVM conditions. While in vivo matured COCs showed abundant levels of these proteins, COCs that underwent IVM had neither detectable Adamts1, nor intact or Adamts1-cleaved Vcan. Human cumulus and granulosa cells matured in vivo contained abundant mRNA for Adamts1 and Vcan, demonstrating the potential relevance to human IVM. CONCLUSION: These results indicate that extensively altered COC matrix composition is present during IVM and may contribute to the observed poorer competence of the derived oocytes. (+info)Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice. (4/273)
BACKGROUND: Successful oocyte in vitro maturation (IVM) would eliminate the need for hormonal stimulation used in assisted reproduction techniques. Unfortunately, oocytes matured in vitro have compromised developmental competence possibly due to disrupted oocyte-cumulus communication resulting from inappropriate levels of oocyte-secreted factors such as growth differentiation factor 9 (GDF9). Hence, the aim of this study was to investigate the effects of exogenous GDF9 during IVM of mouse oocytes on development and subsequent fetal viability. METHODS: Cumulus-oocyte complexes from pregnant mare's serum gonadotrophin primed mice were cultured with or without 200 ng/ml exogenous recombinant GDF9, 50 mIU/ml FSH and 10 ng/ml epidermal growth factor (EGF). After 18 h, cumulus expansion was scored and oocytes were fertilized in vitro. Cleavage, blastocyst development, blastocyst total, inner cell mass (ICM) and trophectoderm cell numbers were assessed. Viability of embryos was assessed by transfer to recipient females and pregnancy outcome determined at day 15. RESULTS: Oocytes matured with exogenous GDF9 in the presence of FSH and EGF had higher rates of development, percentage of hatching blastocyst and blastocyst total and ICM cell numbers (all P < 0.05). Although implantation rate and fetal and placental weights were not affected, the number of viable fetuses at day 15 was increased with exogenous GDF9. CONCLUSIONS: Exogenous GDF9 during IVM improved embryo development and fetal viability and provides a promising approach for human IVM. (+info)Gene silencing of cyclooxygenase-2 mRNA by RNA interference in bovine cumulus-granulosa cells. (5/273)
Inhibition of specific gene expression using RNA interference (RNAi) is a valuable tool for functional analysis of a target gene. However, there is little information available concerning RNAi for analysis of gene function in relation to the reproductive physiology of follicular cells in ruminants. Thus, the aim of this study was to evaluate the interfering effect of small interference RNA (siRNA) on expression of cyclooxygenase-2 (Cox-2) mRNA and prostagrandin F(2alpha) (PGF(2alpha)) production in bovine cumulus-granulosa (CG) cells. Bovine CG cells were collected from aspirated follicles and cultured. After reaching confluency, two experiments were conducted. In experiment 1, to investigate the effective concentration of siRNA, 0, 100, 250 and 500 pM of Cox-2 siRNA was introduced into the CG cells, respectively. After 24 h, the amount of Cox-2 mRNA expression was measured by RT-PCR and real-time PCR. In experiment 2, to investigate the time required for effective interference of siRNA and Cox-2 activity, 250 pM siRNA was introduced for 0, 3, 6, 12 and 24 h. After culture, the amount of Cox-2 mRNA expression was measured and the culture medium was collected to determine the PGF(2alpha) concentration by enzyme immunoassay. The Cox-2 mRNA expression was not affected by introduction of 100 pM siRNA into CG cells for 24 h, but 250 and 500 pM Cox-2 siRNA significantly reduced the Cox-2 mRNA expression. Moreover, the significant suppressive effect of 250 pM siRNA was observed 6 h after introduction, and the reduction of mRNA expression by RNAi became more obvious over 12 h. On the other hand, the PGF(2alpha) concentration in the culture medium was not significantly different 12 h after siRNA introduction; however, the PGF(2alpha) concentration 24 h after siRNA introduction was significantly decreased compared with the control at the same time point. These results suggest that gene silencing of Cox-2 with siRNA is capable of analyzing the function and expression of specific genes in bovine CG cells. (+info)Proteolytic activity of the 26S proteasome is required for the meiotic resumption, germinal vesicle breakdown, and cumulus expansion of porcine cumulus-oocyte complexes matured in vitro. (6/273)
The resumption of oocyte meiosis in mammals encompasses the landmark event of oocyte germinal vesicle (GV) breakdown (GVBD), accompanied by the modification of cell-to-cell communication and adhesion between the oocyte and surrounding cumulus cells. The concomitant cumulus expansion relies on microfilament-cytoskeletal remodeling and extracellular matrix (ECM) deposition. We hypothesized that this multifaceted remodeling event requires substrate-specific proteolysis by the ubiquitin-proteasome pathway (UPP). We evaluated meiotic progression, cytoskeletal dynamics, and the production of cumulus ECM in porcine cumulus-oocyte complexes (COCs) cultured with or without 10-200 microM MG132, a specific proteasomal inhibitor, for the first 22 h of in vitro maturation, followed by 22 h of culture with or without MG132. Treatment with 10 microM MG132 arrested 28.4% of oocytes in GV stage (vs. 1.3% in control), 43.1% in prometaphase I, and 16.2% in metaphase I, whereas 83.7% of control ova reached metaphase II (0% of MG132 reached metaphase II). The proportion of GV-stage ova increased progressively to >90% with increased concentration of MG132 (20-200 microM). Furthermore, MG132 blocked the extrusion of the first polar body and degradation of F-actin-rich transzonal projections (TZP) interconnecting cumulus cells with the oocyte. The microfilament disruptor cytochalasin E (CE) prevented cumulus expansion but accelerated the breakdown of TZPs. Ova treated with a combination of 10 microM MG132 and 10 microM CE underwent GVBD, despite the inhibition of proteasomal activity. However, 90.0% of cumulus-free ova treated with 10 microM MG132 remained in GV stage, compared with 16.7% GV ova in control. Cumulus expansion, retention of hyaluronic acid, and the deposition of cumulus ECM relying on the covalent transfer of heavy chains of inter-alpha trypsin inhibitor (IalphaI) were also inhibited by MG132. Cumulus expansion in control COCs was accompanied by the degradation of ubiquitin-C-terminal hydrolase L3, an important regulator of UPP. RAC1, a UPP-controlled regulator of actin polymerization was maintained at steady levels throughout cumulus expansion. We conclude that proteasomal proteolysis has multiple functions in the progression of oocyte meiosis beyond GV and metaphase I stage, polar body extrusion, and cumulus expansion. (+info)Gene expression in human cumulus cells: one approach to oocyte competence. (7/273)
BACKGROUND: Dialogue between the oocyte and cumulus cells is essential for oocyte maturation. A prospective laboratory research project was designed to evaluate transcription of specific genes in cumulus cells harvested before intracytoplasmic sperm injection from pre-ovulatory follicles, according to individual oocyte nuclear maturity and developmental competence. Genes were chosen because their expression was induced by the LH peak [Steroidogenic Acute Regulatory protein (STAR), Cyclooxygenase 2 (COX2 or PTGS2), Amphiregulin (AREG)] or because they were involved in oocyte lipidic metabolism [Stearoyl-Coenzyme A Desaturase 1 and 5 (SCD1 and SCD5)] or in gap-junctions [Connexin 43 (CX43 or GJA1)]. METHODS: mRNA levels in cumulus cells were assessed by real-time PCR. RESULTS: Expression levels of all genes investigated, except Cx43, were increased after resumption of meiosis. Nuclear maturation was thus associated with increased expression of STAR, COX2, AREG, SCD1 and SCD5 by cumulus cells. When considering only cumulus associated with metaphase II oocytes, gene expression was independent of morphological status at Day 2. In contrast, transcript levels were lower and distributed over a narrower range in cumulus enclosing oocytes achieving blastocyst development at Day 5/6 than in cumulus enclosing oocytes unable to develop beyond the embryo stage. CONCLUSION: Further developmental potential from embryo to blastocyst stage was associated with lower expression in a narrow range for these genes. (+info)The protein profile of mouse mature cumulus-oocyte complex. (8/273)
In mammals, the cumulus-oocyte complex (COC) is the main component of ovarian follicles. Bi-directional communication between oocytes and surrounding cumulus granulosa cells is essential for the development of oocytes and ovarian follicles. In this study, we performed proteomic profiling of mouse mature COC, using two-dimensional gel electrophoresis and mass spectrometry. A total of 259 protein spots were identified, which correspond to 156 individual proteins. We also discovered some protein families, which may play important roles in ovarian follicular development. Immunostaining was conducted to determine the subcellular localization of specific proteins from selected protein families of interest, and to examine their expression patterns during follicle development. These data provide valuable information for future studies to identify proteins involved in ovarian follicular development and related reproductive abnormalities. (+info)
Identification of a novel gene set in human cumulus cells predictive of an oocytes pregnancy potential | Fertility and...
subject:(cumulus oocyte complex) - OATD
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in...
Animal Reproduction | Hiroshima University
10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies Repository of Maulana...
MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation | Journal...
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Aging | Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte...
Paper - Cell-to-cell communication and ovulation - A study of the cumulus-oocyte complex - Embryology
Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a...
Early embryonic development of bovine oocytes challenged with LPS in vitro or in vivo in: Reproduction Volume 158 Issue 5 (2019)
Early embryonic development of bovine oocytes challenged with LPS in vitro or in vivo in: Reproduction Volume 158 Issue 5 (2019)
Cumulus cells surrounding oocytes with high developmental competence exhibit down-regulation of phosphoinositol 1, 3 kinase...
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Use of Both Cumulus Cells Transcriptomic Markers and Zona Pellucida Birefringence to Select Developmentally Competent Oocytes...
Structure of the cumulus matrix and zona pellucida in the golden hamster: a new view of sperm interaction with oocyte...
Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival...
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In vitro and in vivo maturation of llama oocytes
Oocyte competence is independent of the ovulation trigger adopted: a large observational study in a setting that entails...
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Development, Chromosomal Composition, and Cell Allocation of Bovine Cl by G-P. Li, Tom D. Bunch et al.
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"Influences of Cumulus Cells During in vitro Fertilization of Mouse Ooc" by HAYDAR BAĞIŞ, LEVENT KESKİNTEPE et al.
... more blastocyst stage embryos were obtained from cumulus cell enclosed oocytes. Therefore, we suggest that cumulus cells play ... The data demonstrate that more oocytes were arrested at the 2-cell stage in groups that contained no cumulus cells. There was ... demonstrated less polyspermic fertilization and more blastocyst development than those inseminated without any cumulus cells. ... Embryos at the 2-cell stage obtained after in vitro fertilization were transferred to the oviduct of foster mothers to assess ...
Gene expression in human cumulus cells: a way to approach oocyte competence - INRAE - Institut national de recherche pour l...
Palavra-chave » cumulus cells » Somos UNIFESP
Clinical and Molecular Effects of GnRH Agonist and Antagonist on The Cumulus Cells in The In Vitro Fertilization Cycle
Forthis purpose, gene expression of cumulus cells (CCs) as well as clinical and embryological parameters were evaluatedand ... Results: The mean number of cumulus oocyte complex (COC), percentage of metaphase II (MII) oocytes, grade Aembryo and clinical ... Exosomes derived from oviduct cells mediate the EGFR/MAPK signaling pathway in cumulus cells. J Cell Physiol. 2020; 235(2): ... Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal cumulus cells express neuronal and immune- ...
Cyclic AMP-elevating Agents Promote Cumulus Cell Survival and Hyaluronan Matrix Stability, Thereby Prolonging the Time of Mouse...
We show here that, in mice, postovulatory changes of matrix are temporally correlated to cumulus cell death. Cumulus cell ... We show here that, in mice, postovulatory changes of matrix are temporally correlated to cumulus cell death. Cumulus cell ... a process called cumulus cell-oocyte complex (COC) expansion. Hyaluronan release and dispersion of the cumulus cells ... a process called cumulus cell-oocyte complex (COC) expansion. Hyaluronan release and dispersion of the cumulus cells ...
Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome - Embies...
Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome. By The ... Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome To determine ... expression profiles in cumulus cells from polycystic ovarian syndrome (PCOS) patients and identify their potential biological ... NLRP3 in somatic non-immune cells of rodent and primate testes NLRP3 is part of the NLRP3 inflammasome and a global sensor of ...
Volume 76 Issue 3 | Biology of Reproduction
KEYWORDS: AMPK, cumulus cells, granulosa cells, metformin ovary, progesterone, signal transduction, steroid hormones. ... In cumulus cells, leptin treatment increased the mRNA levels for LEPR, STAT3, BAX, BIRC4, and FAS, but did not alter FASLG mRNA ... Leptin Promotes Meiotic Progression and Developmental Capacity of Bovine Oocytes Via Cumulus Cell-Independent and -Dependent ... In conclusion, leptin differentially regulates gene expression in oocytes and cumulus cells. Moreover, leptin enhances both ...
Minimum Essential Medium Eagle Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and...
immature cumulus-oocyte complexes from mice. *human mesenchymal stem cells (MSC). *mouse pre-osteoblastic cell line ... Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations ... With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture ... With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture ...
Ana ISEPPON | Full Professor | Dr. (Ph.D.) | Federal University of Pernambuco, Recife | UFPE | Department of Genetics
MYC integrates FSH signalling networks in cumulus cells during bovine oocyte maturation ... contributes to the acquisition of oocyte competence by modulating signalling pathways in cumulus cells (CCs), albeit much less ... Notwithstanding, such cross-species gene expression inquiries have been scarce for defined cell types and most lack the ... Infectivity of Viable but Non-Cultivable Cells of Pigmented and Nonpigmented Xanthomonas citri pv. anacardii Strains ...
Configure Threshold-Based Event Notifications | Cumulus NetQ 4.1
Rx RoCE PG Usage Cells. Hostname, Interface. Tx RoCE TC Usage Cells. Hostname, Interface. ... NVIDIA Cumulus NetQ 4.1 User Guide*Whats New*Cumulus NetQ 4.1 Open Source Packages ... NVIDIA Cumulus Linux NVIDIA NetQ NVIDIA Cumulus VX Knowledge Base Technical Guides ... cumulus@switch:~$ netq add tca event_id TCA_CPU_UTILIZATION_UPPER scope * channel tca_slack_ifstats threshold 95 This rule ...
Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and...
... how H3K9me3 regulates genome higher-order organization and transcriptional activity in living cells remains unclear. Here, we ... We now confirmed such results in additional mouse cells (MEF vs. cumulus cells [29, 30]) and human cells (hESC vs trophectoderm ... n = 20 cells. d Live cell tracking of C19Q loci movement in EpiGo-KRAB cell lines. The image processing details are the same as ... a Live cell tracking of C19Q dynamics in EpiGo-Control (dCas9) and EpiGo-KRAB (dCas9-KRAB) cell lines. These cell lines were ...
Phone Calls from the 9/11 Airliners: Response to Questions Evoked by My Fifth Estate Interview by Prof David Ray Griffin |...
Cell Phone Limitations. Given the cell phone technology available in 2001, cell phone calls from airliners at altitudes of more ... Cumulus buildups were reported to the pilot. The pilot indicated that he may be overflying the cloud tops. He did not file a ... The Cell Phone Repeater Hypothesis. To review, the main argument used to support the theory that the cell phone calls ... Given that several calls from the jetliner when at altitude were reportedly from cell phones; and that cell phone calls on a ...
Ralph Kazer - Research Output - Northwestern Scholars
Search Results for Journal of the Atmospheric Sciences | AMETSOC
It is shown that this process can exist in the atmosphere and that it can produce sustained convective cells of appreciable ... Nonlinear biconstituent diffusion is proposed as a possible trigger for cumulus development. Properties of this second-order ... It is shown that this process can exist in the atmosphere and that it can produce sustained convective cells of appreciable ... Nonlinear biconstituent diffusion is proposed as a possible trigger for cumulus development. Properties of this second-order ...
Search results - ORA - Oxford University Research Archive
Penetrating Radiation « Climate Audit
Its kind of an inverse of the way that cumulus clouds form during the day. As daylight fades, at some point the ocean stops ... Due to local inequalities, sinking cells form at intervals. These are akin to the thunderstorms, in that they set up cellular ... Finally, the tropical cumulus and cumulonimbus (thunderstorm) clouds are much more prevalent during the daytime than the ...
Dynamic characteristics of lipid metabolism in cultured granulosa cells from geese follicles at different developmental stages ...
Investigations of lipid profile in both follicular cells (including cumulus, granulosa and theca cells) and follicular fluid by ... Cell morphology shows that miR-27b-3p induces cell morphological changes in colorectal cancer cells through phase-contrast ... By comparing the transcriptomic profiling of bovine cumulus cells (CCs, a type of GCs) isolated from ovaries before and after ... MALDI mass spectrometry reveals that cumulus cells modulate the lipid profile of in vitro-matured bovine oocytes ...
Cloning Trevor
... our correspondent spent six months tracking highly experimental work on the cells of a young boy with a life-threatening ... Granted rare access to the labs of Advanced Cell Technology, the only U.S. group openly pursuing human cloning research for ... "cumulus" cell-a type of cell regularly used to clone mice. Unlike skin cells, however, human ovarian cumulus cells are rather ... It comes down to a simple fact about cell division: skin cells, heart cells, liver cells, even adult stem cells, can divide ...
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Aneuploidy in mammalian oocytes and the impact of maternal ageing | Nature Reviews Molecular Cell Biology
Aneuploidy arises as a consequence of aberrant meiosis during egg development from its progenitor cell, the oocyte. In human ... which is much more prevalent in eggs than in either sperm or in most somatic cells. In turn, aneuploidy in eggs is a leading ... impact of maturation and cumulus cells on levels and consumption. J. Cell. Physiol. 229, 353-361 (2014). ... Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells. Nat. Commun. 11, ...
Cloud Image Gallery - Windows to the Universe
Notice that the cirrocumulus cloud cells appear smaller than the cumulus clouds.. (Courtesy of Bob Henson/UCAR) ... This is an image of cumulus clouds.. ( Courtesy of Carlye Calvin) This image of cumulus clouds was taken in the mountains of ... Cumulus. Cumulus (weather symbol - Cu) clouds belong to the Clouds with Vertical Growth group. They are puffy white or light ... View of cumulus clouds from the Great Sand Dunes National Park and Preserve near Alamosa, Colorado.. (Courtesy of Olga and ...
Barry Behr, Ph.D., H.C.L.D.'s Profile | Stanford Profiles
Preliminary study to investigate telomerase reverse transcriptase expression among human cumulus cells. 66th Annual Pacific ... Donation of Embryos for Human Development and Stem Cell Research CELL STEM CELL Kalista, T., Freeman, H. A., Behr, B., Pera, R ... Single-cell gene expression analysis reveals that blastomeres develop cell autonomously, with some cells advancing to EGA and ... MFS cells) are faithfully phenocopied by cells differentiated from induced pluripotent-stem cells derived independently from ...
African Journal of Biotechnology - 20 March 2012 edition
... on the in vitronuclear maturation of canine oocytes and production of progesterone by cumulus cells. cCPE was extracted from ... Cumulus oocyte complexes (COCs) were harvested from anestrus cycle ovaries and.... Author(s): Jin-Dan Kang, Hyo-Sang Lee, ... participating in the immunity and regulating the cell cycle. Using the rapid amplification of cDNA ends (RACE) techniques, the ...
OT receptor | Vasopressin and oxytocin receptors | IUPHAR/BPS Guide to PHARMACOLOGY
1995) Gene expressions of oxytocin and oxytocin receptor in cumulus cells of human ovary. Horm Res, 44 Suppl 2: 47-9. [PMID: ... Can J Biochem Cell Biol, 61 (7): 631-5. [PMID:6313159] 84. Strakova Z, Copland JA, Lolait SJ, Soloff MS. (1998) ERK2 mediates ... Cytogenet Cell Genet, 93 (1-2): 57-9. [PMID:11474180] 32. Elands J, Barberis C, Jard S. (1988) [3H]-[Thr4,Gly7]OT: a highly ... Mol Cell Endocrinol, 124 (1-2): 25-32. [PMID:9027321] 56. Lemaire W, OBrien JA, Burno M, Chaudhary AG, Dean DC, Williams PD, ...
Final Report Summary - MATERNA (Somatic cells regulation of maternal mRNA translation in mammalian oocytes) | FP7 | CORDIS |...
Specifically we observed that de novo transcription in the cumulus cells is required to propagate the EGF-like growth factors- ... Experimental approach: oocytes enclosed in the cumulus cells (CEOs) were treated with hormones/growth factors/signal molecules ... previous studies conducted at the Host Institution showed that EGF-like growth factors initiate a cascade in the cumulus cells ... responsible for signal transduction between cumulus cells and activation of AKT in the oocyte.. SO2.A: a published study ...
Publications | www.ibmc.up.pt
Superoxide dismutase expression in human cumulus oophorus cells. Molecular Human Reproduction, 15(7), 411 - 419.*Google Scholar ... Age-related expression of TGF beta family receptors in human cumulus oophorus cells. Journal of Assisted Reproduction and ... instituto de biologia molecular e celular , institute for molecular and cell biology ...
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These email/text offers will come from Cumulus only. Your information, email address or cell phone number is never sold to a ... CUMULUS MEDIA Inc. ("Cumulus," "we," "us," or "our") is dedicated to preserving your confidence and trust as it relates to the ... Cumulus makes no representation that the Cumulus Platform is governed by or operated in accordance with the laws of any other ... By using the Cumulus Platform, or providing us with any information, you (a) acknowledge that the Cumulus Platform is subject ...
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These email/text offers will come from Cumulus only. Your information, email address or cell phone number is never sold to a ... CUMULUS MEDIA Inc. ("Cumulus," "we," "us," or "our") is dedicated to preserving your confidence and trust as it relates to the ... Cumulus makes no representation that the Cumulus Platform is governed by or operated in accordance with the laws of any other ... By using the Cumulus Platform, or providing us with any information, you (a) acknowledge that the Cumulus Platform is subject ...
Privacy Policy
These email/text offers will come from Cumulus only. Your information, email address or cell phone number is never sold to a ... CUMULUS MEDIA Inc. ("Cumulus," "we," "us," or "our") is dedicated to preserving your confidence and trust as it relates to the ... Cumulus makes no representation that the Cumulus Platform is governed by or operated in accordance with the laws of any other ... By using the Cumulus Platform, or providing us with any information, you (a) acknowledge that the Cumulus Platform is subject ...