AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
Cumulus CellsOocytesOvarian FollicleOogenesisFertilization in VitroBone Morphogenetic Protein 15Growth Differentiation Factor 9Granulosa CellsIn Vitro Oocyte Maturation TechniquesMeiosisFollicular FluidSperm-Ovum InteractionsCloning, OrganismBlastocystOvaryHyaluronoglucosaminidaseFollicle Stimulating HormoneEmbryonic DevelopmentEmbryo Culture TechniquesPhosphofructokinase-1, Type COvulationChorionic GonadotropinCattleZona PellucidaCells, CulturedTGF-beta Superfamily ProteinsSperm Injections, IntracytoplasmicSwineFertilizationNuclear Transfer TechniquesEmbryo TransferSuperovulationGonadotropins, EquineCleavage Stage, OvumSpermatozoaProgesteroneLuteinizing HormoneHyaluronic AcidOocyte RetrievalHeptanolCysteamineConnexin 43GonadotropinsCryoprotective AgentsReceptors, LHCoculture TechniquesPregnancyCell CommunicationBuffaloesGap JunctionsParacrine CommunicationEmbryo, MammalianEpidermal Growth FactorParthenogenesisCulture MediaCryopreservationOvumReceptors, FSHEctogenesisInseminationMitogen-Activated Protein Kinase 14Creatine Kinase, BB FormIntercellular Signaling Peptides and ProteinsOvulation InductionZygoteRNA, MessengerMetaphaseEthylene GlycolEstradiolFallopian TubesMorulaFollicular PhaseVitrificationCell Culture TechniquesButadienesCell NucleusMice, Inbred StrainsGlucuronosyltransferasePregnancy RateEmbryonic and Fetal DevelopmentCulture Media, ConditionedInfertility, FemaleHorsesLuteinizationHypoxanthineMice, Inbred ICRGene ExpressionReverse Transcriptase Polymerase Chain ReactionFollistatinFollicular AtresiaPyruvic AcidCystineInfertilityTime FactorsSperm MotilityAlpha-GlobulinsTissue Culture TechniquesGene Expression Regulation, DevelopmentalShrews
Cumulus Cells
The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. At OVULATION they are extruded with OVUM.
Oocytes
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
Ovarian Follicle
An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS.
Oogenesis
The process of germ cell development in the female from the primordial germ cells through OOGONIA to the mature haploid ova (OVUM).
Fertilization in Vitro
An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.
Bone Morphogenetic Protein 15
A protein that plays a role in GRANULOSA CELLS where it regulates folliculogenesis. Mutations in the gene for bone morphogenetic protein 15 are linked to reproductive abnormalities such as PREMATURE OVARIAN FAILURE.
Growth Differentiation Factor 9
A bone morphogenetic protein that plays an essential role in the regulation of ovarian folliculogenesis.
Granulosa Cells
Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH).
In Vitro Oocyte Maturation Techniques
Methods used to induce premature oocytes, that are maintained in tissue culture, to progress through developmental stages including to a stage that is competent to undergo FERTILIZATION.
Meiosis
A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.
Follicular Fluid
The fluid surrounding the OVUM and GRANULOSA CELLS in the Graafian follicle (OVARIAN FOLLICLE). The follicular fluid contains sex steroids, glycoprotein hormones, plasma proteins, mucopolysaccharides, and enzymes.
Sperm-Ovum Interactions
Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.
Cloning, Organism
The formation of one or more genetically identical organisms derived by vegetative reproduction from a single cell. The source nuclear material can be embryo-derived, fetus-derived, or taken from an adult somatic cell.
Blastocyst
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
Ovary
The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.
Hyaluronoglucosaminidase
An enzyme that catalyzes the random hydrolysis of 1,4-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. (From Enzyme Nomenclature, 1992) There has been use as ANTINEOPLASTIC AGENTS to limit NEOPLASM METASTASIS.
Follicle Stimulating Hormone
A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Follicle-stimulating hormone stimulates GAMETOGENESIS and the supporting cells such as the ovarian GRANULOSA CELLS, the testicular SERTOLI CELLS, and LEYDIG CELLS. FSH consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity.
Embryonic Development
Morphological and physiological development of EMBRYOS.
Embryo Culture Techniques
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
Phosphofructokinase-1, Type C
An allosteric enzyme that regulates glycolysis by catalyzing the transfer of a phosphate group from ATP to fructose-6-phosphate to yield fructose-1,6-bisphosphate. In the humans, 6-phosphofructose-1-kinase isozyme C is found in platelets, brain, heart, kidney, colon and testis. This isozyme C can exist as the homotetramer of C subunits (P subunits), or heterotetramer of C type and L type subunits.
Ovulation
The discharge of an OVUM from a rupturing follicle in the OVARY.
Chorionic Gonadotropin
A gonadotropic glycoprotein hormone produced primarily by the PLACENTA. Similar to the pituitary LUTEINIZING HORMONE in structure and function, chorionic gonadotropin is involved in maintaining the CORPUS LUTEUM during pregnancy. CG consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is virtually identical to the alpha subunits of the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity (CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN).
Cattle
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Zona Pellucida
A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
TGF-beta Superfamily Proteins
A large family of cell regulatory proteins which are structurally related to TRANSFORMING GROWTH FACTOR BETA. The superfamily is subdivided into at least three related protein families: BONE MORPHOGENETIC PROTEINS; GROWTH DIFFERENTIATION FACTORS; and TRANSFORMING GROWTH FACTORS.
Sperm Injections, Intracytoplasmic
An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Fertilization
The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE.
Nuclear Transfer Techniques
Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell.
Embryo Transfer
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
Superovulation
Occurrence or induction of release of more ova than are normally released at the same time in a given species. The term applies to both animals and humans.
Gonadotropins, Equine
Gonadotropins secreted by the pituitary or the placenta in horses. This term generally refers to the gonadotropins found in the pregnant mare serum, a rich source of equine CHORIONIC GONADOTROPIN; LUTEINIZING HORMONE; and FOLLICLE STIMULATING HORMONE. Unlike that in humans, the equine LUTEINIZING HORMONE, BETA SUBUNIT is identical to the equine choronic gonadotropin, beta. Equine gonadotropins prepared from pregnant mare serum are used in reproductive studies.
Cleavage Stage, Ovum
The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.
Spermatozoa
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
Progesterone
The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.
Luteinizing Hormone
A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Luteinizing hormone regulates steroid production by the interstitial cells of the TESTIS and the OVARY. The preovulatory LUTEINIZING HORMONE surge in females induces OVULATION, and subsequent LUTEINIZATION of the follicle. LUTEINIZING HORMONE consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH and FSH), but the beta subunit is unique and confers its biological specificity.
Hyaluronic Acid
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
Oocyte Retrieval
Procedures to obtain viable OOCYTES from the host. Oocytes most often are collected by needle aspiration from OVARIAN FOLLICLES before OVULATION.
Heptanol
A colorless liquid with a fragrant odor. It is used as an intermediate, solvent and in cosmetics.
Cysteamine
A mercaptoethylamine compound that is endogenously derived from the COENZYME A degradative pathway. The fact that cysteamine is readily transported into LYSOSOMES where it reacts with CYSTINE to form cysteine-cysteamine disulfide and CYSTEINE has led to its use in CYSTINE DEPLETING AGENTS for the treatment of CYSTINOSIS.
Connexin 43
A 43-kDa peptide which is a member of the connexin family of gap junction proteins. Connexin 43 is a product of a gene in the alpha class of connexin genes (the alpha-1 gene). It was first isolated from mammalian heart, but is widespread in the body including the brain.
Gonadotropins
Hormones that stimulate gonadal functions such as GAMETOGENESIS and sex steroid hormone production in the OVARY and the TESTIS. Major gonadotropins are glycoproteins produced primarily by the adenohypophysis (GONADOTROPINS, PITUITARY) and the placenta (CHORIONIC GONADOTROPIN). In some species, pituitary PROLACTIN and PLACENTAL LACTOGEN exert some luteotropic activities.
Cryoprotective Agents
Substances that provide protection against the harmful effects of freezing temperatures.
Receptors, LH
Those protein complexes or molecular sites on the surfaces and cytoplasm of gonadal cells that bind luteinizing or chorionic gonadotropic hormones and thereby cause the gonadal cells to synthesize and secrete sex steroids. The hormone-receptor complex is internalized from the plasma membrane and initiates steroid synthesis.
Coculture Techniques
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Pregnancy
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Cell Communication
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Buffaloes
Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus.
Gap Junctions
Connections between cells which allow passage of small molecules and electric current. Gap junctions were first described anatomically as regions of close apposition between cells with a narrow (1-2 nm) gap between cell membranes. The variety in the properties of gap junctions is reflected in the number of CONNEXINS, the family of proteins which form the junctions.
Paracrine Communication
Cellular signaling in which a factor secreted by a cell affects other cells in the local environment. This term is often used to denote the action of INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS on surrounding cells.
Embryo, Mammalian
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
Epidermal Growth Factor
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
Parthenogenesis
A unisexual reproduction without the fusion of a male and a female gamete (FERTILIZATION). In parthenogenesis, an individual is formed from an unfertilized OVUM that did not complete MEIOSIS. Parthenogenesis occurs in nature and can be artificially induced.
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Cryopreservation
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
Ovum
A mature haploid female germ cell extruded from the OVARY at OVULATION.
Receptors, FSH
Cell surface proteins that bind FOLLICLE STIMULATING HORMONE with high affinity and trigger intracellular changes influencing the behavior of cells.
Ectogenesis
Embryonic and fetal development that takes place in an artificial environment in vitro.
Insemination
The deposit of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION.
Mitogen-Activated Protein Kinase 14
A 38-kDa mitogen-activated protein kinase that is abundantly expressed in a broad variety of cell types. It is involved in the regulation of cellular stress responses as well as the control of proliferation and survival of many cell types. The kinase activity of the enzyme is inhibited by the pyridinyl-imidazole compound SB 203580.
Creatine Kinase, BB Form
A form of creatine kinase found in the BRAIN.
Intercellular Signaling Peptides and Proteins
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
Ovulation Induction
Techniques for the artifical induction of ovulation, the rupture of the follicle and release of the ovum.
Zygote
The fertilized OVUM resulting from the fusion of a male and a female gamete.
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Metaphase
The phase of cell nucleus division following PROMETAPHASE, in which the CHROMOSOMES line up across the equatorial plane of the SPINDLE APPARATUS prior to separation.
Ethylene Glycol
A colorless, odorless, viscous dihydroxy alcohol. It has a sweet taste, but is poisonous if ingested. Ethylene glycol is the most important glycol commercially available and is manufactured on a large scale in the United States. It is used as an antifreeze and coolant, in hydraulic fluids, and in the manufacture of low-freezing dynamites and resins.
Estradiol
The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.
Fallopian Tubes
A pair of highly specialized muscular canals extending from the UTERUS to its corresponding OVARY. They provide the means for OVUM collection, and the site for the final maturation of gametes and FERTILIZATION. The fallopian tube consists of an interstitium, an isthmus, an ampulla, an infundibulum, and fimbriae. Its wall consists of three histologic layers: serous, muscular, and an internal mucosal layer lined with both ciliated and secretory cells.
Morula
An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.
Follicular Phase
The period of the MENSTRUAL CYCLE representing follicular growth, increase in ovarian estrogen (ESTROGENS) production, and epithelial proliferation of the ENDOMETRIUM. Follicular phase begins with the onset of MENSTRUATION and ends with OVULATION.
Vitrification
The transformation of a liquid to a glassy solid i.e., without the formation of crystals during the cooling process.
Cell Culture Techniques
Methods for maintaining or growing CELLS in vitro.
Butadienes
Four carbon unsaturated hydrocarbons containing two double bonds.
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Glucuronosyltransferase
A family of enzymes accepting a wide range of substrates, including phenols, alcohols, amines, and fatty acids. They function as drug-metabolizing enzymes that catalyze the conjugation of UDPglucuronic acid to a variety of endogenous and exogenous compounds. EC 2.4.1.17.
Pregnancy Rate
The ratio of the number of conceptions (CONCEPTION) including LIVE BIRTH; STILLBIRTH; and fetal losses, to the mean number of females of reproductive age in a population during a set time period.
Embryonic and Fetal Development
Morphological and physiological development of EMBRYOS or FETUSES.
Culture Media, Conditioned
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Infertility, Female
Diminished or absent ability of a female to achieve conception.
Horses
Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.
Luteinization
Formation of CORPUS LUTEUM. This process includes capillary invasion of the ruptured OVARIAN FOLLICLE, hypertrophy of the GRANULOSA CELLS and the THECA CELLS, and the production of PROGESTERONE. Luteinization is regulated by LUTEINIZING HORMONE.
Hypoxanthine
A purine and a reaction intermediate in the metabolism of adenosine and in the formation of nucleic acids by the salvage pathway.
Mice, Inbred ICR
Gene Expression
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Reverse Transcriptase Polymerase Chain Reaction
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Follistatin
A broadly distributed protein that binds directly to ACTIVINS. It functions as an activin antagonist, inhibits FOLLICLE STIMULATING HORMONE secretion, regulates CELL DIFFERENTIATION, and plays an important role in embryogenesis. Follistatin is a single glycosylated polypeptide chain of approximately 37-kDa and is not a member of the inhibin family (INHIBINS). Follistatin also binds and neutralizes many members of the TRANSFORMING GROWTH FACTOR BETA family.
Follicular Atresia
The degeneration and resorption of an OVARIAN FOLLICLE before it reaches maturity and ruptures.
Pyruvic Acid
An intermediate compound in the metabolism of carbohydrates, proteins, and fats. In thiamine deficiency, its oxidation is retarded and it accumulates in the tissues, especially in nervous structures. (From Stedman, 26th ed)
Cystine
A covalently linked dimeric nonessential amino acid formed by the oxidation of CYSTEINE. Two molecules of cysteine are joined together by a disulfide bridge to form cystine.
Infertility
Inability to reproduce after a specified period of unprotected intercourse. Reproductive sterility is permanent infertility.
Time Factors
Elements of limited time intervals, contributing to particular results or situations.
Sperm Motility
Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.
Alpha-Globulins
Serum proteins that have the most rapid migration during ELECTROPHORESIS. This subgroup of globulins is divided into faster and slower alpha(1)- and alpha(2)-globulins.
Tissue Culture Techniques
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
Gene Expression Regulation, Developmental
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Shrews

Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat. (1/273)

During cryopreservation, the immature oocyte is subjected to anisosmotic conditions potentially impairing subsequent nuclear and cytoplasmic maturation in vitro. In preparation for cryopreservation protocols and to characterize osmotic tolerance, cat cumulus-oocyte complexes (COC) at the germinal vesicle (GV) stage were exposed for 15 min to sucrose solutions ranging from 100 to 2,000 mOsm and then examined for structural integrity and developmental competence in vitro. Osmolarities > or =200 and < or =750 mOsm had no effect on incidence of oocyte nuclear maturation, fertilization success, and blastocyst formation compared to control COC (exposed to 290 mOsm). This relatively high osmotic tolerance of the immature cat oocyte appeared to arise from a remarkable stability of the GV chromatin structure as well as plasticity in mitochondrial distribution, membrane integrity, and ability to maintain cumulus-oocyte communications. Osmolarities <200 mOsm only damaged cumulus cell membrane integrity, which contributed to poor nuclear maturation but ultimately had no adverse effect on blastocyst formation in vitro. Osmolarities >750 mOsm compromised nuclear maturation and blastocyst formation in vitro via disruption of cumulus-oocyte communications, an effect that could be mitigated through 1,500 mOsm by adding cytochalasin B to the hyperosmotic solutions. These results (1) demonstrate, for the first time, the expansive osmotic tolerance of the immature cat oocyte, (2) characterize the fundamental role of cumulus-oocyte communications when tolerance limits are exceeded, and (3) reveal an interesting hyperosmotic tolerance of the immature oocyte that can be increased two-fold by supplementation with cytochalasin B.  (+info)

Reduction of connexin 43 in human cumulus cells yields good embryo competence during ICSI. (2/273)

PURPOSE: The purpose of this study was to predict developmental competence of human oocytes during ICSI via analysis of connexin 43 (Cx43) in cumulus cells surrounding mature oocytes. MATERIALS AND METHODS: Human cumulus cells were manually separated from the oocyte-cumulus complex under a microscope. Cx43 mRNA was expressed by real-time quantitative polymerase chain reaction (RT-PCR) measurement in cumulus cells. RESULTS: There was no significant relationship between expression of Cx43 and fertilisation or cleavage rate. However, Cx43 expression was lower in the good morphology group (blastomeres>7 cells with fragmentation<10% on day 3) when compared to the other groups (p=0.035). CONCLUSIONS: These results suggest that full reduction of Cx43 expression on cumulus cells at the time of oocyte collection during ICSI is essential for developmental competence of human oocytes.  (+info)

Altered composition of the cumulus-oocyte complex matrix during in vitro maturation of oocytes. (3/273)

BACKGROUND: In vitro maturation (IVM) of mammalian oocytes has potential health benefits for patients undergoing assisted reproduction as an alternative to gonadotrophin treatment. This procedure is also useful for studying the process of oocyte and early embryo development. However, oocytes undergoing IVM have much lower competence than in vivo matured oocytes. Efforts to optimize IVM success have focused on replicating in vivo timing, hormonal milieu and cumulus cell responses associated with maturing oocytes. We have previously identified two extracellular matrix proteins, the protease Adamts1 and hyaluronan-binding proteoglycan Versican, produced by mural granulosa cells that selectively incorporate into the periovulatory cumulus-oocyte complex (COC). METHODS: Murine COC were cultured in the presence of epidermal growth factor and/or FSH. mRNA and protein were measured by real time PCR and Western blot and compared to in vivo derived COC. RESULTS: COCs from mice that underwent IVM for 6 or 20 h in the presence of epidermal growth factor, FSH or in combination had a > 10-fold reduction in mRNA (P < 0.05) for Adamts1 and Vcan when compared with in vivo matured COCs. Hyaluronan synthase 2 expression was up-regulated up to 8-fold (P < 0.05) over the unstimulated control, demonstrating successful induction of cumulus gene expression by the IVM conditions. While in vivo matured COCs showed abundant levels of these proteins, COCs that underwent IVM had neither detectable Adamts1, nor intact or Adamts1-cleaved Vcan. Human cumulus and granulosa cells matured in vivo contained abundant mRNA for Adamts1 and Vcan, demonstrating the potential relevance to human IVM. CONCLUSION: These results indicate that extensively altered COC matrix composition is present during IVM and may contribute to the observed poorer competence of the derived oocytes.  (+info)

Exogenous growth differentiation factor 9 in oocyte maturation media enhances subsequent embryo development and fetal viability in mice. (4/273)

BACKGROUND: Successful oocyte in vitro maturation (IVM) would eliminate the need for hormonal stimulation used in assisted reproduction techniques. Unfortunately, oocytes matured in vitro have compromised developmental competence possibly due to disrupted oocyte-cumulus communication resulting from inappropriate levels of oocyte-secreted factors such as growth differentiation factor 9 (GDF9). Hence, the aim of this study was to investigate the effects of exogenous GDF9 during IVM of mouse oocytes on development and subsequent fetal viability. METHODS: Cumulus-oocyte complexes from pregnant mare's serum gonadotrophin primed mice were cultured with or without 200 ng/ml exogenous recombinant GDF9, 50 mIU/ml FSH and 10 ng/ml epidermal growth factor (EGF). After 18 h, cumulus expansion was scored and oocytes were fertilized in vitro. Cleavage, blastocyst development, blastocyst total, inner cell mass (ICM) and trophectoderm cell numbers were assessed. Viability of embryos was assessed by transfer to recipient females and pregnancy outcome determined at day 15. RESULTS: Oocytes matured with exogenous GDF9 in the presence of FSH and EGF had higher rates of development, percentage of hatching blastocyst and blastocyst total and ICM cell numbers (all P < 0.05). Although implantation rate and fetal and placental weights were not affected, the number of viable fetuses at day 15 was increased with exogenous GDF9. CONCLUSIONS: Exogenous GDF9 during IVM improved embryo development and fetal viability and provides a promising approach for human IVM.  (+info)

Gene silencing of cyclooxygenase-2 mRNA by RNA interference in bovine cumulus-granulosa cells. (5/273)

Inhibition of specific gene expression using RNA interference (RNAi) is a valuable tool for functional analysis of a target gene. However, there is little information available concerning RNAi for analysis of gene function in relation to the reproductive physiology of follicular cells in ruminants. Thus, the aim of this study was to evaluate the interfering effect of small interference RNA (siRNA) on expression of cyclooxygenase-2 (Cox-2) mRNA and prostagrandin F(2alpha) (PGF(2alpha)) production in bovine cumulus-granulosa (CG) cells. Bovine CG cells were collected from aspirated follicles and cultured. After reaching confluency, two experiments were conducted. In experiment 1, to investigate the effective concentration of siRNA, 0, 100, 250 and 500 pM of Cox-2 siRNA was introduced into the CG cells, respectively. After 24 h, the amount of Cox-2 mRNA expression was measured by RT-PCR and real-time PCR. In experiment 2, to investigate the time required for effective interference of siRNA and Cox-2 activity, 250 pM siRNA was introduced for 0, 3, 6, 12 and 24 h. After culture, the amount of Cox-2 mRNA expression was measured and the culture medium was collected to determine the PGF(2alpha) concentration by enzyme immunoassay. The Cox-2 mRNA expression was not affected by introduction of 100 pM siRNA into CG cells for 24 h, but 250 and 500 pM Cox-2 siRNA significantly reduced the Cox-2 mRNA expression. Moreover, the significant suppressive effect of 250 pM siRNA was observed 6 h after introduction, and the reduction of mRNA expression by RNAi became more obvious over 12 h. On the other hand, the PGF(2alpha) concentration in the culture medium was not significantly different 12 h after siRNA introduction; however, the PGF(2alpha) concentration 24 h after siRNA introduction was significantly decreased compared with the control at the same time point. These results suggest that gene silencing of Cox-2 with siRNA is capable of analyzing the function and expression of specific genes in bovine CG cells.  (+info)

Proteolytic activity of the 26S proteasome is required for the meiotic resumption, germinal vesicle breakdown, and cumulus expansion of porcine cumulus-oocyte complexes matured in vitro. (6/273)

The resumption of oocyte meiosis in mammals encompasses the landmark event of oocyte germinal vesicle (GV) breakdown (GVBD), accompanied by the modification of cell-to-cell communication and adhesion between the oocyte and surrounding cumulus cells. The concomitant cumulus expansion relies on microfilament-cytoskeletal remodeling and extracellular matrix (ECM) deposition. We hypothesized that this multifaceted remodeling event requires substrate-specific proteolysis by the ubiquitin-proteasome pathway (UPP). We evaluated meiotic progression, cytoskeletal dynamics, and the production of cumulus ECM in porcine cumulus-oocyte complexes (COCs) cultured with or without 10-200 microM MG132, a specific proteasomal inhibitor, for the first 22 h of in vitro maturation, followed by 22 h of culture with or without MG132. Treatment with 10 microM MG132 arrested 28.4% of oocytes in GV stage (vs. 1.3% in control), 43.1% in prometaphase I, and 16.2% in metaphase I, whereas 83.7% of control ova reached metaphase II (0% of MG132 reached metaphase II). The proportion of GV-stage ova increased progressively to >90% with increased concentration of MG132 (20-200 microM). Furthermore, MG132 blocked the extrusion of the first polar body and degradation of F-actin-rich transzonal projections (TZP) interconnecting cumulus cells with the oocyte. The microfilament disruptor cytochalasin E (CE) prevented cumulus expansion but accelerated the breakdown of TZPs. Ova treated with a combination of 10 microM MG132 and 10 microM CE underwent GVBD, despite the inhibition of proteasomal activity. However, 90.0% of cumulus-free ova treated with 10 microM MG132 remained in GV stage, compared with 16.7% GV ova in control. Cumulus expansion, retention of hyaluronic acid, and the deposition of cumulus ECM relying on the covalent transfer of heavy chains of inter-alpha trypsin inhibitor (IalphaI) were also inhibited by MG132. Cumulus expansion in control COCs was accompanied by the degradation of ubiquitin-C-terminal hydrolase L3, an important regulator of UPP. RAC1, a UPP-controlled regulator of actin polymerization was maintained at steady levels throughout cumulus expansion. We conclude that proteasomal proteolysis has multiple functions in the progression of oocyte meiosis beyond GV and metaphase I stage, polar body extrusion, and cumulus expansion.  (+info)

Gene expression in human cumulus cells: one approach to oocyte competence. (7/273)

BACKGROUND: Dialogue between the oocyte and cumulus cells is essential for oocyte maturation. A prospective laboratory research project was designed to evaluate transcription of specific genes in cumulus cells harvested before intracytoplasmic sperm injection from pre-ovulatory follicles, according to individual oocyte nuclear maturity and developmental competence. Genes were chosen because their expression was induced by the LH peak [Steroidogenic Acute Regulatory protein (STAR), Cyclooxygenase 2 (COX2 or PTGS2), Amphiregulin (AREG)] or because they were involved in oocyte lipidic metabolism [Stearoyl-Coenzyme A Desaturase 1 and 5 (SCD1 and SCD5)] or in gap-junctions [Connexin 43 (CX43 or GJA1)]. METHODS: mRNA levels in cumulus cells were assessed by real-time PCR. RESULTS: Expression levels of all genes investigated, except Cx43, were increased after resumption of meiosis. Nuclear maturation was thus associated with increased expression of STAR, COX2, AREG, SCD1 and SCD5 by cumulus cells. When considering only cumulus associated with metaphase II oocytes, gene expression was independent of morphological status at Day 2. In contrast, transcript levels were lower and distributed over a narrower range in cumulus enclosing oocytes achieving blastocyst development at Day 5/6 than in cumulus enclosing oocytes unable to develop beyond the embryo stage. CONCLUSION: Further developmental potential from embryo to blastocyst stage was associated with lower expression in a narrow range for these genes.  (+info)

The protein profile of mouse mature cumulus-oocyte complex. (8/273)

In mammals, the cumulus-oocyte complex (COC) is the main component of ovarian follicles. Bi-directional communication between oocytes and surrounding cumulus granulosa cells is essential for the development of oocytes and ovarian follicles. In this study, we performed proteomic profiling of mouse mature COC, using two-dimensional gel electrophoresis and mass spectrometry. A total of 259 protein spots were identified, which correspond to 156 individual proteins. We also discovered some protein families, which may play important roles in ovarian follicular development. Immunostaining was conducted to determine the subcellular localization of specific proteins from selected protein families of interest, and to examine their expression patterns during follicle development. These data provide valuable information for future studies to identify proteins involved in ovarian follicular development and related reproductive abnormalities.  (+info)

Identification of a novel gene set in human cumulus cells predictive of an oocytes pregnancy potential | Fertility and...Identification of a novel gene set in human cumulus cells predictive of an oocytes pregnancy potential | Fertility and...
This study identified a 12-gene signature in human cumulus cells predictive of pregnancy across multiple clinics. The positive predictive value of our test was 81% with an area under the receiver operating characteristic of 0.763 0.079.
https://www.fertstertdialog.com/posts/8372-identification-of-a-novel-gene-set-in-human-cumulus-cells-predictive-of-an-oocyte-s-pregnancy-potential
subject:(cumulus oocyte complex) - OATDsubject:(cumulus oocyte complex) - OATD
In bovine oocytes, the resumption of meiosis is characterized by the breakdown of the germinal vesicle (GVBD). When cumulus-oocyte complexes (COCs) are cultured in-vitro in the presence of gonadotropins, GVBD is characterized by an initial inhibitory phase, which is followed by an acceleration in the rate of GVBD. An initial transcriptional event is required for gonadotropin-induced in-vitro maturation. The objectives of this thesis were: 1) to define the time course required for transcriptional initiation in bovine cumulus oocyte complexes (COCs); 2) to determine the pattern of expression for Nr4A1 and Egr1 mRNAs in bovine COCs; and 3) to reduce the expression of Nr4A1 mRNA expression to determine its effect on oocyte maturation. Bovine COCs were cultured in the presence follicle stimulating hormone (FSH) alone or FSH with the transcriptional inhibitor, 5,6-dichloro-1-B-Dribofuranosylbenzamidazole (DRB), in order to refine the time course required for transcription initiation and to determine ...
https://oatd.org/oatd/search?q=subject%3A%28cumulus%20oocyte%20complex%29&pagesize=30
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in...Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in...
Comparison of Gene Expression between Cumulus Oocyte Complexes and Naked Oocytes by Suppression Subtractive Hybridization in Swine - Suppression Subtractive Hybridization (SSH);Gene Expression;Cumulus Cells, Porcine;
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1DMBP_2010_v23n1_17
Animal Reproduction | Hiroshima University
Kawashima, I., Liu, Z., Mullany, L.K., Mihara, T., Richards, J.S., Shimada, M. (2012) EGF-like factors induce expansion of the cumulus cell-oocyte complexes by activating calpain-mediated cell movement. Endocrinology 153: 3949-3959.. Noma, N., Kawashima, I., Fan, H.Y, Fujia, Y., Kawai, T., Tomoda, Y., Mihara, T., Richards, J.S., Shimada, M.(2011)LH induces Neuregulin 1 (NRG1) type III specific transcripts that interact with EGF-like factor pathways to control granulosa cell differentiation and oocyte maturation.Mol. Endocrinol. 25: 104-116.. Fujita, Y., Mihara, T., Okazaki, T., Shitanaka, M., Kushino, R., Ikeda, C., Negishi, H., Liu, Z., Richards, J.S., Shimada, M. (2011) Toll-like receptors (TLR) 2 and 4 on human sperm recognize bacterial endotoxins and mediate apoptosis. Hum. Reprod. 26: 2799-2806.. Fan, H.Y., Liu, Z., Shimada, M., Sterneck, E., Johnson, P.F., Hedrick, S.M., Richards, J.S. (2009)ERK1/2 in ovarian granulosa cells are critical for female fertility. Science, 324: 938-941.. ...
https://www.hiroshima-u.ac.jp/en/gsbs/graduateschool/bioresource/animal_reproduction
10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies Repository of Maulana...10-Gingerol as an inducer of apoptosis through HTR1A in cumulus cells: In-vitro and in-silico studies Repository of Maulana...
Objectives Cumulus cells play a crucial role as essential mediators in the maturation of ova. Ginger contains 10-gingerol, which induces apoptosis in colon cancer cells. Based on this hypothesis, this study aimed to determine whether 10-gingerol is able to induce apoptosis in normal cells, namely, cumulus cells. Methods This study used an in vitro analysis by culturing Cumulus cells in M199 containing 10-gingerol in various concentrations (12, 16, and 20 μM) and later detected early apoptotic activity using an Annexin V-FITC detection kit. Result The in vitro data revealed that the number of apoptosis cells increased along with the period of incubation as follows: 12 μM (63.71% ± 2.192%); 16 μM (74.51% ± 4.596%); and 20 μM (78.795% ± 1.435%). The substance 10-gingerol induces apoptosis in cumulus cells by inhibiting HTR1A functions and inactivating GSK3B and AKT-1. Conclusions These findings indicate that further examination is warranted for 10-gingerol as a contraception agent.. ...
http://repository.uin-malang.ac.id/2565/
MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation | Journal...MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation | Journal...
Oocyte maturation and preimplantation embryo development are controlled by array of genes that are post-transcriptionally regulated by microRNAs. With respect to this, previously, we identified altered expression of microRNA-130b (miR-130b) during oocyte maturation. Here, we aimed to investigate the role of miR-130b in bovine granulosa and cumulus cell function, oocyte maturation and preimplantation embryo development using gain- and loss-of- function approach. For this study, the granulosa cells, cumulus cells and the oocytes were collected from ovaries obtained from slaughterhouse. The genes targeted by miR-130b were identified using dual-luciferase reporter assay. The role of miR-130b in granulosa and cumulus cell function was investigated by increasing and inhibiting its expression in in vitro cultured cells using miR-130b precursor and inhibitor, respectively while the role of miR-130b on oocyte development, immature oocytes were microinjected with miR-130b precursor and inhibitor and the polar
https://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-017-0336-1
Influence of cumulus cells on in vitro fertilization of bovine oocytes derived from in vitro maturation<...
TY - JOUR. T1 - Influence of cumulus cells on in vitro fertilization of bovine oocytes derived from in vitro maturation. AU - Chian, R. C.. AU - Okuda, K.. AU - Niwa, K.. PY - 1995/3. Y1 - 1995/3. N2 - The effect of the cumulus cells on in vitro fertilization (IVF) in cattle was evaluated. Frozen-thawed bovine spermatozoa were treated with 5 mM caffeine and 10 μg heparin ml-1 for sperm capacitation. Bovine oocytes with or without cumulus cells after maturation in culture were inseminated by different concentrations of spermatozoa. When the concentration of (0.8-1.5) × 106 spermatozoa ml-1 was employed, the penetration rate was significantly (P , 0.001) higher in cumulus-intact (87%, 130/149) than in cumulus-free (57%, 101/178) oocytes. However, such differences were not observed with sperm concentrations of (2.0-3.0) × 106 and (3.5-5.0) × 106 spermatozoa ml-1 (92% (69/75) vs. 86% (68/79) and 93% (67/72) vs. 91% (73/80) respectively). The polyspermy was significantly (P , 0.05) higher in ...
https://okayama.pure.elsevier.com/en/publications/influence-of-cumulus-cells-on-in-vitro-fertilization-of-bovine-oo
Aging | Circular RNA profiling in the oocyte and cumulus cells reveals that circARMC4 is essential for porcine oocyte...
Thousands of circular RNAs (circRNAs) have been recently discovered in cumulus cells and oocytes from several species. However, the expression and function of circRNA during porcine oocyte meiotic maturation have been never examined. Here, we separately identified 7,067 and 637 circRNAs in both cumulus cells and oocytes via deep sequencing and bioinformatic analysis. Further analysis revealed that a faction of circRNAs is differentially expressed (DE) in a developmental stage-specific manner. The host genes of DE circRNAs are markedly enriched to multiple signaling pathways associated with cumulus cell function and oocyte maturation. Additionally, most DE circRNAs harbor several miRNA targets, suggesting that these DE circRNAs potentially act as miRNA sponge. Importantly, we found that maternal circARMC4 knockdown by siRNA microinjection caused a severely impaired chromosome alignment, and significantly inhibited first polar body extrusion and early embryo development. Taken together, these
https://www.aging-us.com/figure/102315/f2
Paper - Cell-to-cell communication and ovulation - A study of the cumulus-oocyte complex - Embryology
FIGURE 2 Thin section of the cumulus-oocyte complex in a large non-preovulatory follicle from a rat treated with PMSG and HCG. The specimen was taken 10 h after the HCG innennost cumulus layer, the corona radiata, send processes through the zona pelluc contacts on the surface of the oocyte (small arrows) distinguished from the short cumulus cells are also jo ily . The is image, the cumulus processes are eas ifference in staining density . In th lli on the oocyte surface by the d icrovi d to each other by gap ification junctions that can be identified even at low magn ine (large arrows). X 10,000. FIGURES 3-4 Thin-sections of the contact between cumulus cell processes and the oocyte plasma membrane. FIGURE 3 A gap junction is present as a continuous close apposition of the cumulus cell membrane (C) and the oolemma (two arrows). The cytoplasmic surface of the oolemma junctional membrane contains an accumulation of dense material that is not present in the same region of the cumulus cell process. ...
https://embryology.med.unsw.edu.au/embryology/index.php/Paper_-_Cell-to-cell_communication_and_ovulation_-_A_study_of_the_cumulus-oocyte_complex
Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a...Cumulus gene expression as a predictor of human oocyte fertilisation, embryo development and competence to establish a...
The close relationship between cumulus cell function and oocyte developmental competence indicates that analysis of cumulus gene expression is a potential non-invasive method to aid embryo selection and IVF outcome. Cumulus was isolated from 674 oocytes from 75 women undergoing ICSI and gene expression analysed by quantitative RT-PCR. Cumulus expression of cyclooxygenase 2 (PTGS2) was higher with mature oocytes, whereas brain-derived neurotrophic factor (BDNF) was lower when fertilisation was normal. Expression levels of gremlin (GREM1) and BDNF were weak positive and negative predictors of embryo quality respectively. Ranking of GREM1 expression within cohorts of oocytes showed that oocytes associated with the highest GREM1 expression were more likely to be transferred or cryopreserved than discarded (49 vs 33%, P ...
http://temp.crm.ed.ac.uk/publications/cumulus-gene-expression-predictor-human-oocyte-fertilisation-embryo-development-and
Early embryonic development of bovine oocytes challenged with LPS in vitro or in vivo in: Reproduction Volume 158 Issue 5 (2019)Early embryonic development of bovine oocytes challenged with LPS in vitro or in vivo in: Reproduction Volume 158 Issue 5 (2019)
The aim of this study was to evaluate the effect of exposing bovine oocytes to lipopolysaccharides (LPS) in vivo and in vitro on early embryo development. In experiment 1, cumulus oocyte complexes (COCs, n = 700/group) were challenged with 0, 0.1, 1.0 or 5.0 μg/mL of LPS during in vitro maturation (IVM). Later, in vitro fertilization (IVF) and in vitro culture (IVC) were performed. In experiment 2, COCs (n = 200/group) matured and in vitro fertilized without LPS were subjected to IVC with the same doses of LPS from experiment 1. In experiment 3, heifers received two injections of saline solution (n = 8) or 0.5 μg/kg of LPS (n = 8) 24 h apart, and 3 days later, COCs were recovered and submitted to IVM, IVF, and IVC. In experiments 1 and 3, the expression of TLR4, TNF, AREG and EREG genes in cumulus cells was evaluated. Exposure to 1 and 5 μg/mL of LPS during IVM decreased nuclear maturation (39.4 and 39.6%, respectively) compared with control (63.6%, P , 0.05). Despite that, no effect on ...
https://rep.bioscientifica.com/abstract/journals/rep/158/5/REP-19-0316.xml?rskey=ZyMKBI&result=1
Early embryonic development of bovine oocytes challenged with LPS in vitro or in vivo in: Reproduction Volume 158 Issue 5 (2019)Early embryonic development of bovine oocytes challenged with LPS in vitro or in vivo in: Reproduction Volume 158 Issue 5 (2019)
The aim of this study was to evaluate the effect of exposing bovine oocytes to lipopolysaccharides (LPS) in vivo and in vitro on early embryo development. In experiment 1, cumulus oocyte complexes (COCs, n = 700/group) were challenged with 0, 0.1, 1.0 or 5.0 μg/mL of LPS during in vitro maturation (IVM). Later, in vitro fertilization (IVF) and in vitro culture (IVC) were performed. In experiment 2, COCs (n = 200/group) matured and in vitro fertilized without LPS were subjected to IVC with the same doses of LPS from experiment 1. In experiment 3, heifers received two injections of saline solution (n = 8) or 0.5 μg/kg of LPS (n = 8) 24 h apart, and 3 days later, COCs were recovered and submitted to IVM, IVF, and IVC. In experiments 1 and 3, the expression of TLR4, TNF, AREG and EREG genes in cumulus cells was evaluated. Exposure to 1 and 5 μg/mL of LPS during IVM decreased nuclear maturation (39.4 and 39.6%, respectively) compared with control (63.6%, P , 0.05). Despite that, no effect on ...
https://rep.bioscientifica.com/view/journals/rep/158/5/REP-19-0316.xml
Cumulus cells surrounding oocytes with high developmental competence exhibit down-regulation of phosphoinositol 1, 3 kinase...
STUDY QUESTION: Is the phosphoinositol 1, 3-kinase/protein kinase B (PI3K/AKT) pathway expression profile in cumulus cells (CCs) a potential marker of oocyte competence and predictive of pregnancy outcome? SUMMARY ANSWER: Eleven genes (AKT1, ARHGEF7, BCL2L1, CCND1, E2F1, HRAS, KCNH2, PIK3C2A, SHC1, SOS1 and SPP1) in the PI3K/AKT pathway were significantly down-regulated in CCs from oocytes that went on to produce a pregnancy compared to CCs associated with a negative outcome. WHAT IS KNOWN ALREADY: The PI3K/AKT pathway plays a pivotal role in the interdependence and continuous feedback between the oocyte and CCs. STUDY DESIGN SIZE, DURATION: The expression analysis of 92 transcripts in the PI3K/AKT pathway in CCs from patients with negative or positive pregnancy outcome, after single embryo transfer, was performed. Mouse CCs target gene expression was conducted to associate the expression profile of PI3K/AKT pathway to oocyte developmental profile. PARTICIPANTS/MATERIALS, SETTING, METHODS: ...
https://www.iris.unict.it/handle/20.500.11769/321182
Most recent papers with the keyword Oestradiol insulin | Read by QxMDMost recent papers with the keyword Oestradiol insulin | Read by QxMD
The objective of the present study was to investigate the cumulus expansions of Nili Ravi buffalo oocytes during cultured in TCM-199 supplemented with 2 μg/ml oestradiol (E(2)), 0.05 IU/ml recombinant human follicle stimulating hormone (rhFSH), 2IU/ml human chorionic gonadotrophin (hCG), and 0.12 IU/ml insulin (I). The cumulus oocytes complexes (COCs) were collected from 2-8mm follicles from local abattoir ovaries. Supplementation of medium with single hormones showed significant (P,0.0001) increase in mean diameter of COCs with rhFSH except E(2), hCG and insulin after 24 hours compared to the increase in the mean diameter of COCs matured in TCM-199 without any hormonal supplementation ...
https://www.readbyqxmd.com/keyword/15442
Publications : CRDSI
Intercellular gap-junctional communication (GJC) plays an important role in ovarian cell physiology. Closure of GJC has been proposed to be involved in oocyte maturation, particularly in the resumption of meiosis, both in vivo and in vitro, by controlling the flow of meiosis inhibitors, such as cAMP and cGMP. Understanding how GJC dynamics are regulated during in vitro maturation (IVM) could provide a powerful tool for controlling meiotic resumption and oocyte maturation in vitro. Since little is known about the GJC dynamic regulation between cumulus cells, we have developed an assay based on recovery of calcein fluorescence in photobleached cumulus cells, a gap-FRAP assay. The GJC profile has been characterized during the first hours of porcine IVM. We showed that equine chorionic gonadotropin (eCG) and epidermal growth factor (EGF) down-regulated GJC effectiveness between cumulus cells. However, human chorionic gonadotropin was not down-regulating GJC effectiveness. We also showed that the GJC ...
https://www.crdsi.ulaval.ca/index.php?id=12&L=1%23c17896%27%5B0%5D%20&id=12&tx_fsaacentrerecherche_gestionpublication%5BauteurSelected%5D=16&tx_fsaacentrerecherche_gestionpublication%5Baction%5D=show&tx_fsaacentrerecherche_gestionpublication%5Bcontroller%5D=Publication&tx_fsaacentrerecherche_gestionpublication%5B%40widget_0%5D%5BcurrentPage%5D=2&cHash=ba93b4ec7fc0a636018e63bcf7ce2861&tx_fsaacentrerecherche_gestionpublication%5Bpublication%5D=216
Cumulus Evaluation, Oocyte Competence and MiRNA - International IVF InitiativeCumulus Evaluation, Oocyte Competence and MiRNA - International IVF Initiative
Cumulus Evaluation, Oocyte Competence and MiRNA DR. ALISON BARTOLUCCI Alison is the Director of Laboratory Operations at the Center for Advanced Reproductive Services at the University of Connecticut. Prior to becoming a
https://ivfmeeting.com/products/cumulus-evaluation-oocyte-competence-and-mirna
GEL CUMULUS 19 HOMMEGEL CUMULUS 19 HOMME
ASICS GEL-CUMULUS 19 rouge/noir, GEL CUMULUS 19 HOMME La gel cumulus 19 est la chausse de haute performance idéal pour les coureurs de demi-fond et de fond. Cette nouvelle version de la gel cumulus est faite pour une course sur route quelle que soit la cadence.
https://www.sportpro-online.com/asics-cumulus-xml-230-2778.html
SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)
We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in ...
https://www.sabiia.cnptia.embrapa.br/sabiia/search?search=creatorFacets:%22PONTELO,%20T.%20P.%22&qFacets=creatorFacets:%22PONTELO,%20T.%20P.%22&sort=titleSort&paginacao=t&paginaAtual=1
Once Optimized it Will Provide Effective Alternative to Standard Controlled Ovarian Stimulation
Association between the proportion of dominant follicles and oocyte developmental competence. Li, Yi; Li, Rui-Qi; Ou, Song-Bang; Ren, Ling; Zhang, Ning-Feng; Wei, Li-Na; Zhang, Qing-Xue; Yang, Dong-Zi // Journal of Assisted Reproduction & Genetics;Dec2014, Vol. 31 Issue 12, p1599 Purpose: To explore the optimal timing for hCG triggering by investigating the impact of different proportion of dominant follicles on the oocyte developmental competence. Methods: One hundred ninety-eight infertile women were divided into three groups according to the proportion of dominant... ...
http://connection.ebscohost.com/c/articles/86433778/once-optimized-will-provide-effective-alternative-standard-controlled-ovarian-stimulation
Spec Property Developments - Cumulus Apartments - Real Estate Agency ProfileSpec Property Developments - Cumulus Apartments - Real Estate Agency Profile
Residential real estate for sale & lease by Spec Property Developments - Cumulus Apartments. Also view Sold listings, agents, contact details and the office location of Spec Property Developments - Cumulus Apartments.
https://www.realestate.com.au/agency/spec-property-developments-cumulus-apartments-BTEHKD
Cumulus Cells and their Associations with Immune Functions - Fingerprint - Johns Hopkins University
Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2021 Elsevier B.V We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. ...
https://jhu.pure.elsevier.com/en/publications/cumulus-cells-and-their-associations-with-immune-functions/fingerprints/
We in this study have been able to locate the cumulus i | Open-iWe in this study have been able to locate the cumulus i | Open-i
We in this study have been able to locate the cumulus in 94.6% of the cases in conception cycles and in 53% of non-conception cycles by surface rendering [Table
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2800929_JHRS-02-62-g008&req=4
Verify Network Connectivity | Cumulus NetQ 4.0Verify Network Connectivity | Cumulus NetQ 4.0
[email protected]:~$ netq show ip neighbors Matching neighbor records: IP Address Hostname Interface MAC Address VRF Remote Last Changed ------------------------- ----------------- ------------------------- ------------------ --------------- ------ ------------------------- ... 192.168.200.1 server04 eth0 44:38:39:00:00:6d default no Tue Nov 3 19:50:23 2020 10.1.10.1 server04 uplink 00:00:00:00:00:1a default no Tue Nov 3 19:50:23 2020 10.1.10.101 server04 uplink 44:38:39:00:00:32 default no Tue Nov 3 19:50:23 2020 10.1.10.2 server04 uplink 44:38:39:00:00:5d default no Tue Nov 3 19:50:23 2020 10.1.10.3 server04 uplink 44:38:39:00:00:5e default no Tue Nov 3 19:50:23 2020 192.168.200.250 server04 eth0 44:38:39:00:01:80 default no Tue Nov 3 19:50:23 2020 192.168.200.1 server03 eth0 44:38:39:00:00:6d default no Tue Nov 3 19:50:22 2020 192.168.200.250 server03 eth0 44:38:39:00:01:80 default no Tue Nov 3 19:50:22 2020 192.168.200.1 server02 eth0 44:38:39:00:00:6d default no Tue Nov 3 19:50:22 2020 ...
https://docs.nvidia.com/networking-ethernet-software/cumulus-netq-40/Verify-Network-Connectivity/
Cumulus - Orchard Road - #02-14 Wheelock PlaceCumulus - Orchard Road - #02-14 Wheelock Place
See 5 photos from 433 visitors about man bags, bag shop, and staff. They have a good range of bags that are both functional and fashionable. Ranging...
https://foursquare.com/v/cumulus/4bd6eafa29eb9c74bbc895e1
Corporate Contest Rules Archive | KMAJ-FMCorporate Contest Rules Archive | KMAJ-FM
Pursuant to FCC Amends Section 73.1216 of the Commissions Rules, the following information and rules links pertain to specific Cumulus corporate contesting run on this and other Cumulus stations.. Pick Your Pearl Jam ...
http://www.kmaj.com/1111/11/11/corporate-contest-rules-archive/
RIF.orgRIF.org
Clouds come in all sorts of types, including cumulus, cumulonimbus, stratus, and cirrus. Learn how to tell each cloud type apart.. ...
https://www.rif.org/literacy-central/search/latest/field_genres/fiction-159/field_themes/weather-160/supported_grades/1st-243?sort=created&order=desc&field_themes=All&type=All
Helens Vlogs - Board Meeting March 2020 | Network HomesHelens Vlogs - Board Meeting March 2020 | Network Homes
Weve just published the latest video update from the Board with Helen Evans, our Chief Executive summarising the main topics discussed.
https://www.networkhomes.org.uk/news/latest-news/blogs/helens-vlog-board-meeting-march-2020/
Adelaide Research & Scholarship: Oocyte-secreted factor activation of SMAD 2/3 signaling enables initiation of mouse cumulus...Adelaide Research & Scholarship: Oocyte-secreted factor activation of SMAD 2/3 signaling enables initiation of mouse cumulus...
Expansion of the mouse cumulus-oocyte complex (COC) is dependent on oocyte-secreted paracrine factors. Transforming growth factor beta (TGFB) superfamily molecules are prime candidates for the cumulus expansion-enabling factors (CEEFs), and we have recently determined that growth differentiation factor 9 (GDF9) alone is not the CEEF. The aim of this study was to examine oocyte paracrine factors and their signaling pathways that regulate mouse cumulus expansion. Using RT-PCR, oocytes were found to express the two activin subunits, Inhba and Inhbb, and activin A and activin B both enabled FSH-induced cumulus expansion of oocytectomized (OOX) complexes. Follistatin, an activin-binding protein, neutralized activin-induced expansion but had no effect on oocyte-induced expansion. The type I receptors for GDF9 and activin are activin receptor-like kinase 5 (ALK5) and ALK4, respectively, both of which activate the same SMAD 2/3 signaling pathway. We examined the requirement for this signaling system ...
https://digital.library.adelaide.edu.au/dspace/handle/2440/43460
SearchSearch
We investigated the effect mouse cumulus cells (mCCs) on the in vitro maturation (IVM) and developmental potential of bovine denuded germinal vesicle oocytes (DOs). Cumulus-oocyte complexes (COCs), DOs and DOs cocultured with either mCCs (DOs + mCCs) or bovine cumulus cells (bCCs; DOs + bCCs) were subjected to IVM. The meiosis II (MII) rates of DOs, glutathione (GSH) contents, zona pellucida (ZP) hardening and parthenogenetic blastocyst rates of MII oocytes were determined. The relative expression levels of bone morphogenetic protein 15 (BMP-15) and growth differentiation factor 9 (GDF-9) in MII oocytes were measured using quantitative real-time polymerase chain reaction (PCR). mCCs significantly increased the MII rate of DOs from 53.5 ± 3.58% to 69.67 ± 4.72% (p , 0.05) but had no effect on the GSH content (2.17 ± 0.31 pmol/oocyte with mCCs, 2.14 ± 0.53 pmol/oocyte without mCCs). For the DOs + mCCs group, the BMP-15 and GDF-9 expression levels were significantly higher and the ZP ...
https://www.cambridge.org/core/search?filters%5BauthorTerms%5D=Hai%20Ren&eventCode=SE-AU
Use of Both Cumulus Cells Transcriptomic Markers and Zona Pellucida Birefringence to Select Developmentally Competent Oocytes...Use of Both Cumulus Cells Transcriptomic Markers and Zona Pellucida Birefringence to Select Developmentally Competent Oocytes...
Selection of the best oocyte for subsequent steps of fertilization and embryo transfer was shown to be the crucial step in human infertility treatment procedure. Oocyte selection using morphological criteria mainly Zona pellucida (ZP) has been the gold standard method in assisted reproductive technologies (ART) clinics, but this selection approach has limitations in terms of accuracy, objectivity and constancy. Recent studies using OMICs-based approaches have allowed the identification of key molecular markers that quantitatively and non-invasively predict the oocyte quality for higher pregnancy rates and efficient infertility treatment. These biomarkers are a valuable reinforcement of the morphological selection criteria widely used in in vitro fertilization (IVF) clinics. In this context, this study was designed to investigate the relationship between transcriptomic predictors of oocyte quality found by our group and the conventional morphological parameters of oocyte quality mainly the ZP
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-16-S1-S9
Structure of the cumulus matrix and zona pellucida in the golden hamster: a new view of sperm interaction with oocyte...Structure of the cumulus matrix and zona pellucida in the golden hamster: a new view of sperm interaction with oocyte...
Structure of the cumulus matrix and zona pellucida in the golden hamster: a new view of sperm interaction with oocyte-associated extracellular matrices.
https://scholars.duke.edu/display/pub779736
Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival...Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival...
Complete denudation of oocytes before slush nitrogen vitrification does not influence survival rates but positively affects oocyte meiotic spindle competence. These data support the hypothesis that cumulus cells during vitrification represent an obstacle to cryoprotectant penetration more than having a protective role for the oocyte.. ...
http://fertstertforum.com/2012971220minasi/
January | 2018 | SE Farm
Oocytes are generally recovered as a cumulus-oocyte complex, an oocyte surrounded by the cumulus cells that nourish it, dislodged from the follicle wall. Initially as a follicle grows, the cumulus cells are tightly adhered to the follicle wall and the mass of cells itself is very dense; the cells are very close together. As the follicle grows and becomes a dominant follicle the cumulus mass starts to expand as the cells secrete an extracellular matrix that spreads them apart. Nearing ovulation this expansion makes the attachment to the follicle wall weaker and weaker so that during ovulation the oocyte can be swept from the follicle into the oviduct. The loosening of the attachment makes the oocyte somewhat easier to recover nearing ovulation, although as the follicle enlarges it is more difficult to create the turbulence necessary to dislodge the oocyte. For these reasons, aspiration of the immature follicles with their firmly attached cumulus-oocyte complex, tend to have the highest recovery ...
http://sefarm.com/2018/01/
Uncategorized | SE Farm
Oocytes are generally recovered as a cumulus-oocyte complex, an oocyte surrounded by the cumulus cells that nourish it, dislodged from the follicle wall. Initially as a follicle grows, the cumulus cells are tightly adhered to the follicle wall and the mass of cells itself is very dense; the cells are very close together. As the follicle grows and becomes a dominant follicle the cumulus mass starts to expand as the cells secrete an extracellular matrix that spreads them apart. Nearing ovulation this expansion makes the attachment to the follicle wall weaker and weaker so that during ovulation the oocyte can be swept from the follicle into the oviduct. The loosening of the attachment makes the oocyte somewhat easier to recover nearing ovulation, although as the follicle enlarges it is more difficult to create the turbulence necessary to dislodge the oocyte. For these reasons, aspiration of the immature follicles with their firmly attached cumulus-oocyte complex, tend to have the highest recovery ...
http://sefarm.com/category/uncategorized/
Gap junctional conversation (GJC) takes on a primordial part in oocyte | down-regulation of Mcl-1 via a proteasomal-dependent...Gap junctional conversation (GJC) takes on a primordial part in oocyte | down-regulation of Mcl-1 via a proteasomal-dependent...
Gap junctional conversation (GJC) takes on a primordial part in oocyte maturation and meiotic resumption in mammals by directing the transfer of several substances between cumulus cells as well as the oocyte. gonadotropins, recommending that it could be the main regulator of GJC. Furthermore, the degradation of Cx43 indicated after 4.5 h of IVM in response to equine chorionic gonadotropin seemed to involve the proteasomal complex. Cx43 localisation were connected with GJC. Used together, these outcomes show for the very first time that gonadotropins control Cx43 protein manifestation, degradation and localisation in porcine COC through the first a long time of IVM. Rules of Cx43 may subsequently, via GJC, take part in the introduction of completely competent oocytes. Intro Gap junctional conversation (GJC) plays important roles in various cells and cell types, including ovaries, follicles and cumulus oocyte complicated (COC). Certainly, GJC is usually involved in many physiological and ...
https://q-vd-oph.com/gap-junctional-conversation-gjc-takes-on-a-primordial-part-in-oocyte/
Original article(s) | Vlaams Diergeneeskundig Tijdschrift
Between 1996 and 2000 79 Belgian blue donor cows were submitted to OPU-IVF. They all had a history in classical embryo transfer programs with disappointing results. Two different in vitro embryo production protocols were used. Between 1996 and 1998 (period A), oocytes were matured in M199 and Epidermal Growth Factor (EGF) and Foetal Calf Serum (FCS) were added. Subsequently, the oocytes were cultured in a granulosa co-culture system in a Synthetic Oviduct Fluid (SOF) medium. From 1998 until 2000 (Period B), M199, FCS, equine chorionic gonatrophin (eCG) and a granulosa cell co-culture were used for in vitro maturation.Zygotes were subsequently cultured in SOF with a co-culture of bovine oviduct epithelial cells (BOEC). During period A, 531 OPU-IVF sessions were performed, collecting 2111 cumulus oocyte complexes (COCs), of which 928 (44 %) were of good morphological quality. The In vitro maturation (IVM), fertilization (IVF) and culture (IVC) resulted in 241 transferable embryos. When fresh ...
http://vdt.ugent.be/?q=en/taxonomy/term/110&page=6
Team : CRDSI
My research program investigates the oocyte, better known as ovule, but in its immature state. Our team is especially interested in cell signalling since it plays a primordial role in the gametes maturation. Even more specifically, we investigate the role of cyclic nucleotide enzyme degradation, namely phosphodiesterases (PDEs). We have made interesting discovering by identifying new families of PDEs found in cumulus cells, some of which seem to be regulated through their association with membrane lipids of the raft family and others which are very sensitive to cAMP regulation. We are also interested in understanding the dialogue between cumulus cells and the oocyte. We have developed a tools for the functional evaluation of cumulus cells response by measuring Gap junction communication using fluorophores and photobleaching. Thanks to this approach, we are among world leaders involved in demonstrating the high level of regulation of gap junctions during in vitro maturation. We are very ...
https://www.crdsi.ulaval.ca/en/team/afficher_personne/details/richard-francois-j/
Spam1 - Hyaluronidase PH-20 precursor - Rattus norvegicus (Rat) - Spam1 gene & proteinSpam1 - Hyaluronidase PH-20 precursor - Rattus norvegicus (Rat) - Spam1 gene & protein
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid (By similarity).
http://www.uniprot.org/uniprot/Q62803
Dead Cell Removal Kit | Removal reagents | MACS Sample Preparation | Products | Miltenyi Biotec | Belgique
The Dead Cell Removal Kit is a fast and straightforward way of eliminating dead cells from cell cultures or tissue preparations. The Dead Cell Removal Kit contains ready-to-use MicroBeads and Binding Buffer for the magnetic labeling of cell debris, dead cells, and dying cells. The magnetically labeled material is removed by magnetic separation and pure, viable cells are obtained within 25 minutes. | Belgique
https://www.miltenyibiotec.com/BE-en/products/dead-cell-removal-kit.html
Single-center Prospective Cumulus Cell Test Study in rFSH Patients - Full Text View - ClinicalTrials.govSingle-center Prospective Cumulus Cell Test Study in rFSH Patients - Full Text View - ClinicalTrials.gov
This observation is routinely performed by the study nurses of the fertility center for every patient undergoing standard ART treatment and is thus available from the fertility center database. This measurement does not include a scale, there is either a child born or not. The date of delivery and the gender of the child are asked together with eventual complications ...
https://www.clinicaltrials.gov/ct2/show/NCT03663868
In vitro and in vivo maturation of llama oocytesIn vitro and in vivo maturation of llama oocytes
Cumulus-oocyte complexes (COC) were collected from abbatoir-derived llama ovaries and cultured in vitro for 28, 30, or 36 h at 39 degrees C in 5% CO2 to determine the time required for maturation. The majority of COC (n = 298, 87%) were classified as categories I and 2 (COC with ,= 5 layers or 2-4 compact layers of cumulus cells, respectively) and homogeneous ooplasm, and the proportion that underwent nuclear maturation (MII) was 78, 81 and 80%, after 28, 30 and 36 h, respectively (P = 0.65). To compare the effectiveness of FSH versus eCG for inducing in vivo maturation, in experiment 2, llamas (n = 20 per group) were treated with: (1) 25 mg FSH, twice-daily for 4 day, plus 5 mg armour of LH at the end of FSH treatment; or (2) 1000 IU of eCG, plus 5 mg armour of LH 4 day after eCG treatment. The FSH- and eCG-treated groups did not differ (P = 0.85) with respect to the number of follicles ,= 6 mm at the time of COC collection (17.9 +/- 2.2 versus 17.7 +/- 2.2), the number of COC collected (10.7 ...
http://repositoriodigital.uct.cl/handle/10925/3476
Oocyte competence is independent of the ovulation trigger adopted: a large observational study in a setting that entails...Oocyte competence is independent of the ovulation trigger adopted: a large observational study in a setting that entails...
To assess whether the GnRH-agonist or urinary-hCG ovulation triggers affect oocyte competence in a setting entailing vitrified-warmed euploid blastocyst tr
https://link.springer.com/article/10.1007/s10815-021-02124-1?error=cookies_not_supported&code=c7b0d594-08a6-4efb-bfed-b3f067a4d85c
Search
BACKGROUND: Oocytes are the female gametes which establish the program of life after fertilization. Interactions between oocyte and the surrounding cumulus cells at germinal vesicle (GV) stage are considered essential for ...
http://ir.library.msstate.edu/handle/11668/5430/discover?filtertype=subject&filter_relational_operator=equals&filter=Animals
Search
BACKGROUND: Oocytes are the female gametes which establish the program of life after fertilization. Interactions between oocyte and the surrounding cumulus cells at germinal vesicle (GV) stage are considered essential for ...
http://ir.library.msstate.edu/handle/11668/5431/discover?filtertype=type&filter_relational_operator=equals&filter=Article
Savage Hemorrhages Hannitys Audience: New Yorks WABC Loses 50%, Chicago Down 59%Savage Hemorrhages Hannitys Audience: New Yorks WABC Loses 50%, Chicago Down 59%
Its the kind of conservative media story only conservatives understand. The latest numbers are out for Michael Savages radio show. Recall that Savage was a key player in the battle between Sean Hannity and Cumulus radio. A battle that had an exasperated Hannity finally firing Cumulus, as reported here in NewsBusters at the time. But not before Savage, whom Cumulus had in the
https://www.newsbusters.org/blogs/nb/jeffrey-lord/2014/05/17/savage-hemorrhages-hannitys-audience-new-yorks-wabc-loses-50
Explore our research
Vettese, S., Thompson, P. & Allan, D. (2017). A study of the multi generational relationship with making through mediated designing in collaborative, digital environments: implications for learning and open access fabrication. In Cumulus Hong Kong 2016, Cumulus Working Papers, Open Design for E-very-thing, 23 - 30. ISBN 9789526000800. ...
https://www.napier.ac.uk/research-and-innovation/research-search?q=KTP%20Soluis%20Group%20Ltd
AerosolsAerosols
Today will be a special one in the desert. Cumulus bases are going to be really LOW for summer, maybe only 3-4 kft above the ground, and likely warmer than 15 C (50 F). Maybe 50 F doesnt sound special, but it is. A base temperature of summer clouds that warm is rarely observed here. And with that, and all the posts here about the temperatures that ice forms (around -10 C, 14 F) are out the window. Ice will form at much higher temperatures than usual.. This is because on a day when the Cumulus bases are that warm, rain forms by collisions between droplets before clouds even reach the -5 C (23 F) level, the highest temperature at which Ma Nature can produce ice. Rain mgiht even form in our clouds today even before the freezing level itself!. This is so exciting for an Arizonan who has studied ice-in-clouds development over the years because today ice will form in clouds around the -5 to -10 C level, and the mechanism of Mssrs Hallett and Mossop will be heavily involved as well as other lesser ...
http://cloud-maven.com/category/aerosols/
Cub Scout Weather - MeritBadgeDotOrgCub Scout Weather - MeritBadgeDotOrg
2. Explain how clouds are made. Describe the different kinds of clouds-stratus, cumulus, cumulonimbus, and cirrus-and what kind of weather can be associated with these cloud types ...
http://meritbadge.org/wiki/index.php?title=Cub_Scout_Weather&oldid=9428
COC 8007F-04物性表资料最新价格,COC 8007F-04物性表资料物性表,SGS报告查询-塑胶物性网
塑胶物性网为您提供COC 8007F-04物性表资料价格,COC 8007F-04物性表资料 物性表,SGS报告查询;了解更多信息,COC,8007F,04!
http://sujiaowuxing.com/thread-108775-1.html
Validating reference microRNAs for normalizing qRT-PCR data in bovine oocytes and preimplantation embryos | BMC Developmental...Validating reference microRNAs for normalizing qRT-PCR data in bovine oocytes and preimplantation embryos | BMC Developmental...
MicroRNAs (miRNAs) are small noncoding RNAs that act as post-transcriptional regulators of gene targets. Accurate quantification of miRNA expression using validated internal controls should aid in the understanding of their role in epigenetic modification of genome function. To date, most studies that have examined miRNA expression levels have used the global mean expression of all expressed genes or the expression of reference mRNAs or nuclear RNAs for normalization. We analyzed the suitability of a number of miRNAs as potential expression normalizers in bovine oocytes and early embryos, and porcine oocytes. The stages examined were bovine oocytes at the germinal vesicle (GV) and metaphase II stages, bovine zygotes, 2, 4 and 8 cell embryos, morulae and blastocysts, as well as porcine cumulus oocyte complexes, GV, metaphase I and II oocytes. qRT-PCR was performed to quantify expression of miR-93, miR-103, miR-26a, miR-191, miR-23b, Let-7a and U6 for bovine samples and miR-21, miR-26a, miR-93, miR-103,
https://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-015-0075-8
JCI - Parthenogenetic stem cells for tissue-engineered heart repairJCI - Parthenogenetic stem cells for tissue-engineered heart repair
Generation and culture of PSCs. We induced superovulation in 6- to 8-week-old (C57BL/6J × DBA/2J)F1 (B6D2F1) mice by i.p. injection of 7.5 U pregnant mare serum gonadotropin (PMSG) and, 48 hours later, 7.5 U human choriogonadotropin (hCG). We harvested oviducts and collected cumulus oocyte complexes (COCs) 16 hours after hCG injection. Cumulus cells were removed with hyaluronidase (1 mg/ml for 10 minutes at room temperature). We subsequently activated oocytes with strontium chloride (10 mmol/l in Ca2+-free CZB medium in mmol/l: NaCl 82.7, KCl 4.68, KH2PO4 1.17, MgSO4 1.18, D-glucose 5.6, Na-lactate 30.1, EDTA2Na 0.1, NaHCO3 25, Na-pyruvate 0.62, glutamine 1; supplemented with 5 mg/ml BSA, and 100 U/ml penicillin and 100 μg/ml streptomycin [100 P/S]) for 6 hours and prevented second polar body extrusion with cytochalasin B (5 μg/ml). Blastocysts formed during culture in mineral oil-covered Ca2+-containing (1.71 mmol/l) CZB medium for 6 days. We transferred blastocysts onto mitomycin C (10 ...
https://www.jci.org/articles/view/66854
Development, Chromosomal Composition, and Cell Allocation of Bovine Cl by G-P. Li, Tom D. Bunch et al.Development, Chromosomal Composition, and Cell Allocation of Bovine Cl by G-P. Li, Tom D. Bunch et al.
Treatment of in vitro matured bovine oocytes with colcemid results in a membrane protrusion that contains maternal chromosomes, which can be easily removed by aspiration. Four experiments were designed to evaluate the overall and temporal effects of conditioned medium (CM) by bovine cumulus cells on development of nuclear transfer (NT) bovine embryos and to examine the chromosomal composition and allocation of inner cell mass (ICM) and trophectoderm (TE) of the subsequent blastocysts. The nuclear transfer embryos were cultured in various CR1aa media conditioned by preculture with bovine cumulus cells. Development to the blastocyst stage in BSA-containing CM (BCM) and serum-containing CM (SCM) were similar to co-culture group (24-30%). The 24 hr-conditioned BCM yielded higher blastocyst development than 48 and 72 hr-conditioned BCM. Temporary exposure of embryos to BCM and SCM followed by CR1aa was also studied. Morula and blastocyst development were not different among the groups cultured in BCM for 72,
https://digitalcommons.usu.edu/advs_facpub/420/
Effects of ovarian stimulation on oocyte development and embryo quality - Nottingham ePrintsEffects of ovarian stimulation on oocyte development and embryo quality - Nottingham ePrints
Ovarian stimulation plays a pivotal role in assisted reproductive therapies, to increase the number of embryos available for treatment; however, there is no clear consensus from meta-analyses in the literature which, if any, of the preparations in use are superior in terms of clinical outcomes. The aim of this thesis was to examine the effect of common human gonadotrophin preparations with different half lives and LH activity (hMG, rFSH and Pergoveris) on embryo quality and resulting offspring, compared to non- stimulated negative controls and positive PMSG treated controls, using the mouse model. The studies in this thesis indicated that an LH ceiling threshold is evident during folliculogenesis, where the use of long acting LH preparations resulted in higher numbers of fragmented oocytes, absent of cumulus cells (P,0.001), reduced expression of the pro and anti-angiogenic factors, MYHII and PEDF in cumulus cells (P,0.05), increased embryonic developmental arrest (P,0.001) and perturbed IGF2 ...
http://eprints.nottingham.ac.uk/14233/
Media Confidential: Memphis Radio: Morgan Bohannon New VP/MM For CumulusMedia Confidential: Memphis Radio: Morgan Bohannon New VP/MM For Cumulus
Bohannon, a Memphis native, joins Cumulus from iHeartMedia-Memphis, where he was most recently Area President. He served iHeartMedia/Clear Channel Radio in various capacities and markets for over 20 years, was previously Market Manager for iHeartMedia-Memphis; Director of Sales for iHeartMedia in Greenville, SC; and Regional Vice President for Clear Channel Radio-Charlotte, NC. Bohannon was also previously Director of Sales for Apex Broadcasting. Bohannon is Board Chair for both The First Tee of Memphis and Junior Achievement of The Mid-South and is a Member of the Board of Directors of The Consortium MMT ...
http://mediaconfidential.blogspot.com/2017/04/memphis-radio-morgan-bohannon-new-vpmm.html
Avaliação da expressão gênica e da maturação nuclear in vitro em complexos cumuli-oócitos bovinosAvaliação da expressão gênica e da maturação nuclear in vitro em complexos cumuli-oócitos bovinos
Dentre os principais desafios que persistem no campo da biologia da reprodução está a compreensão da natureza dos processos celulares e moleculares que determinam a qualidade dos oócitos. Um dos fenômenos a serem melhor compreendidos é a aquisição da competência do oócito, e qual o papel desempenhado pelo ambiente folicular que circunda o gameta no seu potencial de desenvolvimento. As células foliculares, especialmente as células do cumulus, certamente desempenham um papel fundamental na aquisição da competência de oócitos in vivo. Durante a maturação in vitro (MIV) do oócito observa-se expansão e mucificação das células da granulosa que formam o complexo cumulus oophorus-oócito (CCO), em função da intensa síntese de componentes da matriz extracelular. Essas modificações no aspecto do cumulus são utilizadas como indicativo da ocorrência de maturação oocitária e contribuem para que ocorra a fecundação. A expressão de proteínas associadas à matriz ...
https://lume.ufrgs.br/handle/10183/34778?locale-attribute=es
Σύγκριση έτους Woodbridge - meteoblueΣύγκριση έτους Woodbridge - meteoblue
Low, mid and high cloud cover: Cloud cover at different altitudes. High clouds (8-15 km) like cirrus are less significant for total cloud cover than low (below 4 km) like stratus, cumulus and fog or mid clouds (4-8 km) like alto cumulus and alto stratus ...
https://www.meteoblue.com/el/%CE%BA%CE%B1%CE%B9%CF%81%CF%8C%CF%82/archive/yearcomparison/woodbridge_%CE%97%CE%BD%CF%89%CE%BC%CE%AD%CE%BD%CE%B5%CF%82-%CE%A0%CE%BF%CE%BB%CE%B9%CF%84%CE%B5%CE%AF%CE%B5%CF%82-%CF%84%CE%B7%CF%82-%CE%91%CE%BC%CE%B5%CF%81%CE%B9%CE%BA%CE%AE%CF%82_5106529
123 Best Nike Operating Shoes (December 2018) | Live War
The Gel Kayano, Gel Cumulus, and GT 2000 are few shoe models from Asics that basically made a reputation in the operating trade. The adidas name dates again to 1948, deriving from the primary two syll
http://livewar.com/123-best-nike-operating-shoes-december-2018-2/
Influences of Cumulus Cells During in vitro Fertilization of Mouse Ooc by HAYDAR BAĞIŞ, LEVENT KESKİNTEPE et al."Influences of Cumulus Cells During in vitro Fertilization of Mouse Ooc" by HAYDAR BAĞIŞ, LEVENT KESKİNTEPE et al.
... more blastocyst stage embryos were obtained from cumulus cell enclosed oocytes. Therefore, we suggest that cumulus cells play ... The data demonstrate that more oocytes were arrested at the 2-cell stage in groups that contained no cumulus cells. There was ... demonstrated less polyspermic fertilization and more blastocyst development than those inseminated without any cumulus cells. ... Embryos at the 2-cell stage obtained after in vitro fertilization were transferred to the oviduct of foster mothers to assess ...
https://journals.tubitak.gov.tr/veterinary/vol25/iss5/22/
Gene expression in human cumulus cells: a way to approach oocyte competence - INRAE - Institut national de recherche pour l...Gene expression in human cumulus cells: a way to approach oocyte competence - INRAE - Institut national de recherche pour l...
Gene expression in human cumulus cells: a way to approach oocyte competence Dominique Royère 1, 2 Prisca P. Feuerstein 1, 2 ... Gene expression in human cumulus cells: a way to approach oocyte competence. 63. Annual Meeting of American Society for ...
https://hal.inrae.fr/hal-02756347
Palavra-chave » cumulus cells » Somos UNIFESPPalavra-chave » cumulus cells » Somos UNIFESP
Palavra-chave: cumulus cells utilizada 11 vezes por 3 professores. Utilizada por 3 professores Por ordem de relevância (total: ... Palavra-chave relacionada é aquela que foi utilizada juntamente com "cumulus cells" ...
https://somos.unifesp.br/palavra_chave/cumulus-cells
Clinical and Molecular Effects of GnRH Agonist and Antagonist on The Cumulus Cells in The In Vitro Fertilization CycleClinical and Molecular Effects of GnRH Agonist and Antagonist on The Cumulus Cells in The In Vitro Fertilization Cycle
Forthis purpose, gene expression of cumulus cells (CCs) as well as clinical and embryological parameters were evaluatedand ... Results: The mean number of cumulus oocyte complex (COC), percentage of metaphase II (MII) oocytes, grade Aembryo and clinical ... Exosomes derived from oviduct cells mediate the EGFR/MAPK signaling pathway in cumulus cells. J Cell Physiol. 2020; 235(2): ... Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal cumulus cells express neuronal and immune- ...
https://www.ijfs.ir/article_239411.html
Cyclic AMP-elevating Agents Promote Cumulus Cell Survival and Hyaluronan Matrix Stability, Thereby Prolonging the Time of Mouse...Cyclic AMP-elevating Agents Promote Cumulus Cell Survival and Hyaluronan Matrix Stability, Thereby Prolonging the Time of Mouse...
We show here that, in mice, postovulatory changes of matrix are temporally correlated to cumulus cell death. Cumulus cell ... We show here that, in mice, postovulatory changes of matrix are temporally correlated to cumulus cell death. Cumulus cell ... a process called cumulus cell-oocyte complex (COC) expansion. Hyaluronan release and dispersion of the cumulus cells ... a process called cumulus cell-oocyte complex (COC) expansion. Hyaluronan release and dispersion of the cumulus cells ...
https://art.torvergata.it/handle/2108/142433
Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome - Embies...Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome - Embies...
Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome. By The ... Characterization of circular RNA expression profiles in cumulus cells from patients with polycystic ovary syndrome To determine ... expression profiles in cumulus cells from polycystic ovarian syndrome (PCOS) patients and identify their potential biological ... NLRP3 in somatic non-immune cells of rodent and primate testes NLRP3 is part of the NLRP3 inflammasome and a global sensor of ...
https://www.embies.com/characterization-of-circular-rna-expression-profiles-in-cumulus-cells-from-patients-with-polycystic-ovary-syndrome/
Volume 76 Issue 3 | Biology of ReproductionVolume 76 Issue 3 | Biology of Reproduction
KEYWORDS: AMPK, cumulus cells, granulosa cells, metformin ovary, progesterone, signal transduction, steroid hormones. ... In cumulus cells, leptin treatment increased the mRNA levels for LEPR, STAT3, BAX, BIRC4, and FAS, but did not alter FASLG mRNA ... Leptin Promotes Meiotic Progression and Developmental Capacity of Bovine Oocytes Via Cumulus Cell-Independent and -Dependent ... In conclusion, leptin differentially regulates gene expression in oocytes and cumulus cells. Moreover, leptin enhances both ...
https://bioone.org/journals/biology-of-reproduction/volume-76/issue-3
Minimum Essential Medium Eagle Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and...Minimum Essential Medium Eagle Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and...
immature cumulus-oocyte complexes from mice. *human mesenchymal stem cells (MSC). *mouse pre-osteoblastic cell line ... Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations ... With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture ... With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture ...
https://www.sigmaaldrich.com/JP/en/product/sigma/m4526
Ana ISEPPON | Full Professor | Dr. (Ph.D.) | Federal University of Pernambuco, Recife | UFPE | Department of GeneticsAna ISEPPON | Full Professor | Dr. (Ph.D.) | Federal University of Pernambuco, Recife | UFPE | Department of Genetics
MYC integrates FSH signalling networks in cumulus cells during bovine oocyte maturation ... contributes to the acquisition of oocyte competence by modulating signalling pathways in cumulus cells (CCs), albeit much less ... Notwithstanding, such cross-species gene expression inquiries have been scarce for defined cell types and most lack the ... Infectivity of Viable but Non-Cultivable Cells of Pigmented and Nonpigmented Xanthomonas citri pv. anacardii Strains ...
https://www.researchgate.net/profile/Ana-Iseppon
Configure Threshold-Based Event Notifications | Cumulus NetQ 4.1Configure Threshold-Based Event Notifications | Cumulus NetQ 4.1
Rx RoCE PG Usage Cells. Hostname, Interface. Tx RoCE TC Usage Cells. Hostname, Interface. ... NVIDIA Cumulus NetQ 4.1 User Guide*Whats New*Cumulus NetQ 4.1 Open Source Packages ... NVIDIA Cumulus Linux NVIDIA NetQ NVIDIA Cumulus VX Knowledge Base Technical Guides ... cumulus@switch:~$ netq add tca event_id TCA_CPU_UTILIZATION_UPPER scope * channel tca_slack_ifstats threshold 95 This rule ...
https://docs.nvidia.com/networking-ethernet-software/cumulus-netq-41/Manage-Configuration/Manage-Events-and-Notifications/Config-TCA-Event-Notif/
Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and...Simultaneous epigenetic perturbation and genome imaging reveal distinct roles of H3K9me3 in chromatin architecture and...
... how H3K9me3 regulates genome higher-order organization and transcriptional activity in living cells remains unclear. Here, we ... We now confirmed such results in additional mouse cells (MEF vs. cumulus cells [29, 30]) and human cells (hESC vs trophectoderm ... n = 20 cells. d Live cell tracking of C19Q loci movement in EpiGo-KRAB cell lines. The image processing details are the same as ... a Live cell tracking of C19Q dynamics in EpiGo-Control (dCas9) and EpiGo-KRAB (dCas9-KRAB) cell lines. These cell lines were ...
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-020-02201-1
Phone Calls from the 9/11 Airliners: Response to Questions Evoked by My Fifth Estate Interview by Prof David Ray Griffin |...Phone Calls from the 9/11 Airliners: Response to Questions Evoked by My Fifth Estate Interview by Prof David Ray Griffin |...
Cell Phone Limitations. Given the cell phone technology available in 2001, cell phone calls from airliners at altitudes of more ... Cumulus buildups were reported to the pilot. The pilot indicated that he may be overflying the cloud tops. He did not file a ... The Cell Phone Repeater Hypothesis. To review, the main argument used to support the theory that the cell phone calls ... Given that several calls from the jetliner when at altitude were reportedly from cell phones; and that cell phone calls on a ...
http://911blogger.com/node/22369
Ralph Kazer - Research Output - Northwestern Scholars
Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality. Zhang, X., ...
https://www.scholars.northwestern.edu/en/persons/ralph-kazer/publications/
Search Results for Journal of the Atmospheric Sciences | AMETSOCSearch Results for Journal of the Atmospheric Sciences | AMETSOC
It is shown that this process can exist in the atmosphere and that it can produce sustained convective cells of appreciable ... Nonlinear biconstituent diffusion is proposed as a possible trigger for cumulus development. Properties of this second-order ... It is shown that this process can exist in the atmosphere and that it can produce sustained convective cells of appreciable ... Nonlinear biconstituent diffusion is proposed as a possible trigger for cumulus development. Properties of this second-order ...
https://journals.ametsoc.org/search?access=all&f_0=author&q_0=Joseph+T.+Schaefer&t=atsc
Search results - ORA - Oxford University Research ArchiveSearch results - ORA - Oxford University Research Archive
Oocyte-specific ablation of N- and O-glycans alters cumulus cell signalling and extracellular matrix composition ...
https://ora.ox.ac.uk/objects/?f%5Bf_contributors__name%5D%5B%5D=Lo,%20B
Penetrating Radiation « Climate AuditPenetrating Radiation « Climate Audit
Its kind of an inverse of the way that cumulus clouds form during the day. As daylight fades, at some point the ocean stops ... Due to local inequalities, sinking cells form at intervals. These are akin to the thunderstorms, in that they set up cellular ... Finally, the tropical cumulus and cumulonimbus (thunderstorm) clouds are much more prevalent during the daytime than the ...
https://climateaudit.org/2005/05/22/penetrating-radiation/
Dynamic characteristics of lipid metabolism in cultured granulosa cells from geese follicles at different developmental stages ...Dynamic characteristics of lipid metabolism in cultured granulosa cells from geese follicles at different developmental stages ...
Investigations of lipid profile in both follicular cells (including cumulus, granulosa and theca cells) and follicular fluid by ... Cell morphology shows that miR-27b-3p induces cell morphological changes in colorectal cancer cells through phase-contrast ... By comparing the transcriptomic profiling of bovine cumulus cells (CCs, a type of GCs) isolated from ovaries before and after ... MALDI mass spectrometry reveals that cumulus cells modulate the lipid profile of in vitro-matured bovine oocytes ...
https://portlandpress.com/bioscirep/article/39/12/BSR20192188/221432/Dynamic-characteristics-of-lipid-metabolism-in
Cloning TrevorCloning Trevor
... our correspondent spent six months tracking highly experimental work on the cells of a young boy with a life-threatening ... Granted rare access to the labs of Advanced Cell Technology, the only U.S. group openly pursuing human cloning research for ... "cumulus" cell-a type of cell regularly used to clone mice. Unlike skin cells, however, human ovarian cumulus cells are rather ... It comes down to a simple fact about cell division: skin cells, heart cells, liver cells, even adult stem cells, can divide ...
https://www.theatlantic.com/magazine/archive/2002/06/cloning-trevor/302508/
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Aneuploidy in mammalian oocytes and the impact of maternal ageing | Nature Reviews Molecular Cell BiologyAneuploidy in mammalian oocytes and the impact of maternal ageing | Nature Reviews Molecular Cell Biology
Aneuploidy arises as a consequence of aberrant meiosis during egg development from its progenitor cell, the oocyte. In human ... which is much more prevalent in eggs than in either sperm or in most somatic cells. In turn, aneuploidy in eggs is a leading ... impact of maturation and cumulus cells on levels and consumption. J. Cell. Physiol. 229, 353-361 (2014). ... Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells. Nat. Commun. 11, ...
https://www.nature.com/articles/s41580-022-00517-3?error=cookies_not_supported&code=e1135117-0e99-44de-9e2a-442b614fd1ab
Cloud Image Gallery - Windows to the UniverseCloud Image Gallery - Windows to the Universe
Notice that the cirrocumulus cloud cells appear smaller than the cumulus clouds.. (Courtesy of Bob Henson/UCAR) ... This is an image of cumulus clouds.. ( Courtesy of Carlye Calvin) This image of cumulus clouds was taken in the mountains of ... Cumulus. Cumulus (weather symbol - Cu) clouds belong to the Clouds with Vertical Growth group. They are puffy white or light ... View of cumulus clouds from the Great Sand Dunes National Park and Preserve near Alamosa, Colorado.. (Courtesy of Olga and ...
https://windows2universe.org/earth/Atmosphere/clouds/cloud_il.html&edu=high&dev=1&lang=en
Barry Behr, Ph.D., H.C.L.D.s Profile | Stanford ProfilesBarry Behr, Ph.D., H.C.L.D.'s Profile | Stanford Profiles
Preliminary study to investigate telomerase reverse transcriptase expression among human cumulus cells. 66th Annual Pacific ... Donation of Embryos for Human Development and Stem Cell Research CELL STEM CELL Kalista, T., Freeman, H. A., Behr, B., Pera, R ... Single-cell gene expression analysis reveals that blastomeres develop cell autonomously, with some cells advancing to EGA and ... MFS cells) are faithfully phenocopied by cells differentiated from induced pluripotent-stem cells derived independently from ...
https://profiles.stanford.edu/barry-behr?tab=publications
African Journal of Biotechnology - 20 March 2012 editionAfrican Journal of Biotechnology - 20 March 2012 edition
... on the in vitronuclear maturation of canine oocytes and production of progesterone by cumulus cells. cCPE was extracted from ... Cumulus oocyte complexes (COCs) were harvested from anestrus cycle ovaries and.... Author(s): Jin-Dan Kang, Hyo-Sang Lee, ... participating in the immunity and regulating the cell cycle. Using the rapid amplification of cDNA ends (RACE) techniques, the ...
https://academicjournals.org/journal/AJB/edition/20_March_2012
OT receptor | Vasopressin and oxytocin receptors | IUPHAR/BPS Guide to PHARMACOLOGYOT receptor | Vasopressin and oxytocin receptors | IUPHAR/BPS Guide to PHARMACOLOGY
1995) Gene expressions of oxytocin and oxytocin receptor in cumulus cells of human ovary. Horm Res, 44 Suppl 2: 47-9. [PMID: ... Can J Biochem Cell Biol, 61 (7): 631-5. [PMID:6313159] 84. Strakova Z, Copland JA, Lolait SJ, Soloff MS. (1998) ERK2 mediates ... Cytogenet Cell Genet, 93 (1-2): 57-9. [PMID:11474180] 32. Elands J, Barberis C, Jard S. (1988) [3H]-[Thr4,Gly7]OT: a highly ... Mol Cell Endocrinol, 124 (1-2): 25-32. [PMID:9027321] 56. Lemaire W, OBrien JA, Burno M, Chaudhary AG, Dean DC, Williams PD, ...
https://www.guidetopharmacology.org/GRAC/ObjectDisplayForward?objectId=369
Final Report Summary - MATERNA (Somatic cells regulation of maternal mRNA translation in mammalian oocytes) | FP7 | CORDIS |...Final Report Summary - MATERNA (Somatic cells regulation of maternal mRNA translation in mammalian oocytes) | FP7 | CORDIS |...
Specifically we observed that de novo transcription in the cumulus cells is required to propagate the EGF-like growth factors- ... Experimental approach: oocytes enclosed in the cumulus cells (CEOs) were treated with hormones/growth factors/signal molecules ... previous studies conducted at the Host Institution showed that EGF-like growth factors initiate a cascade in the cumulus cells ... responsible for signal transduction between cumulus cells and activation of AKT in the oocyte.. SO2.A: a published study ...
https://cordis.europa.eu/project/id/624874/reporting/de
Publications | www.ibmc.up.pt
Superoxide dismutase expression in human cumulus oophorus cells. Molecular Human Reproduction, 15(7), 411 - 419.*Google Scholar ... Age-related expression of TGF beta family receptors in human cumulus oophorus cells. Journal of Assisted Reproduction and ... instituto de biologia molecular e celular , institute for molecular and cell biology ...
https://www.ibmc.up.pt/research/publications?page=9&s=title&%3Bf%5Bag%5D=C&%3Bf%5Bauthor%5D=1857&f%5Bauthor%5D=1885&o=asc
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