Vero cell

OPV AIDS hypothesis

Ptk2 cells

Botany

Montrose Thomas Burrows

Chicken as biological research model

Nina Starr Braunwald

Somaclonal variation

Semecarpus anacardium

Two-hybrid screening

Lilium

Smith-Lemli-Opitz syndrome

Bartonella henselae

In vitro

Mikimoto Kōkichi

Rockefeller University

Medical microbiology

Biceps

Brucella

Knee cartilage replacement therapy

Stem-cell therapy

Stem-cell therapy

Astrocyte

Infection

Biceps

Bio-MEMS

Telomere

Plastic surgery

Medicine

Vivo16

• Furthermore, these techniques can be used to generate implantable constructs with defined architecture and controlled cell distribution, allowing for in vitro and in vivo investigation of cell interactions in a 3D environment. (nih.gov)
• It was noted that as opposed to the flattened morphology typically seen in 2D culture, cells grown on the electrospun fibers exhibited a more rounded 3-dimensional morphology generally observed of tissues in vivo. (wikipedia.org)
• Plant Tissue Culture is way to grow whole plant from any part in vivo. (mattvaller.com)
• Unlike 2D environments (e.g. a petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. (wikipedia.org)
• Standard 2D, or monolayer, cell cultures are inadequate representations of this environment, which often makes them unreliable predictors of in vivo drug efficacy and toxicity. (wikipedia.org)
• 3D spheroids more closely resemble in vivo tissue in terms of cellular communication and the development of extracellular matrices. (wikipedia.org)
• As the natural extracellular matrix (ECM) is important in the survival, proliferation, differentiation and migration of the cells, different hydrogel matrices mimicking natural ECM structure are considered as potential approaches towards in vivo -like cell culturing. (wikipedia.org)
• To date, most in vitro and in vivo studies in the field of cardiovascular tissue research rely on the conventional monolayer (2D) cell cultures. (frontiersin.org)
• Such 2D culture systems may introduce false positive and/or negative results in the mechanistic studies and translational applications primarily due to the microenvironment of 2D cultures that substantially differ from the in vivo cardiovascular cellular and extracellular matrix (ECM) organizations. (frontiersin.org)
• Recently, it is found that transition from conventional monolayer cell cultures to 3D culture systems contributes to a closer recapitulation of in vivo features, such as cell heterogeneity, ECM, cell signalling, proliferation, maturation, and response to stimuli. (frontiersin.org)
• For example, the engineered heart tissue/muscle and cardiovascular spheroids have shown great promises in the in vitro modelling of familial cardiomyopathies, cardiovascular toxicity assessment, drug discovery, and in vivo cardiac regeneration (i.e., cardiac patch). (frontiersin.org)
• 3D culturing has become popular in the field of drug-screening and toxicity, as the cells respond to chemicals in a more ' in vivo way' than 2D cultures. (labx.com)
• Balls of interacting cells form 'organoids' or 'organ spheroids' that replicate tissues in vivo . (labx.com)
• Cell culture systems that better recapitulate the physiological conditions and environment a cell experiences in vivo can improve our understanding of cellular behaviour. (nature.com)
• It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (harvard.edu)
• Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth. (harvard.edu)

Cultivation of tissues1

• The most significant innovation described in their early publications was probably the method designed for serial cultivation of tissues. (asu.edu)

Mammalian6

• The two biologists also expanded the application of tissue culture to tissues of various types, including adult tissues, tissues of mammalian origin, and cancerous tissues from chicks and humans . (asu.edu)
• At CLS, she led the development of various new products including advanced extracellular matrices/surface coatings and laboratory devices for culture of mammalian primary and stem cells. (corning.com)
• Covers methods for creating mammalian fibroblastic cell cultures and maintaining culture conditions for epithelial, neuronal, and hematopoietic cells among others. (barnesandnoble.com)
• 1 Basic Techniques for Mammalian Cell Tissue Culture UNIT 1.1 Tissue culture technology has found wide application in the field of cell biology. (docplayer.net)
• This method was found to be effective for culturing mammalian hepatocytes. (labx.com)
• Many types of mammalian cells can aggregate and differentiate into 3-D multicellular spheroids when cultured in suspension or a nonadhesive environment. (wiley.com)

Fibroblasts4

• Aging of Cultured Human Skin Fibroblasts. (barnesandnoble.com)
• This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types including Human Foreskin Fibroblasts (HFF), transformed Human Carcinoma (HEp-2), and Mink Lung Epithelium (MLE) would adhere to and proliferate upon the fibers. (wikipedia.org)
• In addition to being able to source adequate tissue-specific human cells for our bioengineered constructs, incorporation of supportive cells (e.g. endothelial cells and fibroblasts) as well as structural matrix proteins can allow for recapitulation of the dynamic interactions of cells with each other and with the cellular microenvironment. (nature.com)
• In the 1960s, biologists found that normal human fibroblasts, cells derived from connective tissue , had a predictable limit in their ability to proliferate in culture. (encyclopedia.com)

Epithelial6

• Based on previous publications on storage of cultured epithelial cells [ 20 - 23 ], we hypothesize that differences in storage temperature between 4°C and 37°C affect the viability, morphology, and phenotype of cultured RPE cells. (hindawi.com)
• This group has recently shown that epithelial cells extracted from the developing teeth of 6-month-old pigs continue to proliferate when they are cultured on top of a special feeder layer of cells (the feeder-layer cells are known as the 3T3-J2 cell line). (eurekalert.org)
• In the study being reported today, the researchers seeded the cultured dental epithelial cells onto collagen sponge scaffolds, along with cells from the middle of the tooth (dental mesenchymal cells). (eurekalert.org)
• The key finding of this study was that even after the multiple divisions that occurred during propagation of the cells in culture, the dental epithelial cells retained the ability to produce enamel, as long as they were later provided with an appropriate environment. (eurekalert.org)
• The idea for the culturing technique originates from 1975, when Dr. J.G. Rheinwald and Dr. H. Green of Harvard Medical School reported the use of feeder layers for culturing epithelial cells from the skin (the 3T3-J2 cells used in the current study were gifted by Dr. Green). (eurekalert.org)
• Now that dental epithelial cells can be propagated in culture, the next step will be to achieve the same success with their partners in tooth formation, the dental mesenchymal cells. (eurekalert.org)

Laboratory17

• In: Vasil IK (ed) Cell culture and somatic cell genetics of plants: volume 1: laboratory procedures and their applications. (springer.com)
• In 1910, Carrel's assistant, Montrose Burrows, visited Harrison's laboratory at Yale University for several months to learn about tissue culture. (asu.edu)
• More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. (worldcat.org)
• Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition , addresses the most current principles and methods in plant tissue culture research. (routledge.com)
• New topic discussions and laboratory exercises in the Second Edition include "Micropropagation of Dieffenbachia," "Micropropagation and in vitro flowering of rose," "Propagation from nonmeristematic tissue-organogenesis," "Variation in culture" and "Tissue culture of ferns. (routledge.com)
• In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition , is a veritable harvest of information for the continued study and research in plant tissue culture science. (routledge.com)
• The appeal of this practical manual is that it contains very detailed information on how to establish a tissue culture laboratory, from provision of lists of equipment and chemicals and their suppliers, to procedures and protocols for preparing media and carrying out experiments…I would recommend this book highly for teachers and researchers in plant tissue culture as a valuable resource for practical plant tissue culture. (routledge.com)
• In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. (springer.com)
• The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. (springer.com)
• The artificial growth of sheets of human tissue in the laboratory. (thefreedictionary.com)
• Learn to propagate plants using micropropagation techniques in a laboratory. (mattvaller.com)
• Many different recipes for tissue culture media are available and each laboratory must determine which medium best suits their needs. (docplayer.net)
• Early studies in the 80's, led by Mina Bissell from the Lawrence Berkeley National Laboratory, highlighted the importance of 3D techniques for creating accurate in vitro culturing models. (wikipedia.org)
• For these reasons, there is a need to focus efforts on establishing best practices for collecting fresh tissue samples from wild organisms outside of the laboratory, which maximizes the utility of material and reduces the number of individuals that need to be collected. (jove.com)
• Tissue culture is one of the most widespread laboratory techniques. (selectscience.net)
• Cell culture describes the laboratory growth of cells derived from plants or animals. (encyclopedia.com)
• The ability to culture cells allowed the laboratory growth of polio virus to produce vaccines that nearly eliminated polio as a disease. (encyclopedia.com)

Organism8

• This serial cultivation was also the basis of Carrel's 1912 paper "On the Permanent Life of Tissues outside of the Organism. (asu.edu)
• Cultures prepared directly from the tissue o{ an organism arc called prlmuy cultures. (expertsmind.com)
• 2. An acid-sensitive, but facultative acid-fast and pleomorphic organism did grow in certain of these cultures. (ajtmh.org)
• Tissue culture is the growth of tissues or cells separate from the organism. (wikipedia.org)
• In modern usage, tissue culture generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
• enrichment culture one grown on a medium, usually liquid, that has been supplemented to encourage the growth of a given type of organism. (thefreedictionary.com)
• 1. The technique or process of maintaining or cultivating cells or tissues derived from a living organism in a culture medium. (thefreedictionary.com)
• Harrison's methods were originally developed to study the conditions promoting differentiation of nerve fibers, and his techniques were later were modified to study the effects of salts and various animal extracts on animal tissue growth independent and separate from the animal organism. (golden.com)

Ross Granvill1

• Carrel's work with tissue culture followed the research of Ross Granville Harrison . (asu.edu)

Monolayer4

• TRYPSINIZING AND SUBCULTURING CELLS FROM A MONOLAYER A primary culture is grown to confluency in a 60-mm petri plate or 25-cm 2 tissue culture flask containing 5 ml tissue culture medium. (docplayer.net)
• There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models. (biotek.com)
• or Monolayer that is attached to the tissue culture flask e.g. (powershow.com)
• Compared to conventional monolayer cultures, multicellular spheroids resemble real tissues better in terms of structural and functional properties. (wiley.com)

Organisms7

• and, on the other hand, to the interpretation of the acid-sensitive organisms appearing in the cultures as contaminants. (ajtmh.org)
• The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. (elsevier.com)
• Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. (wikipedia.org)
• stab culture a culture into which the organisms are introduced by thrusting a needle deep into the medium. (thefreedictionary.com)
• As high-throughput sequencing technologies advance, standardized methods for high quality tissue acquisition and preservation allow for the extension of these methods to non-model organisms. (jove.com)
• Cellular agriculture is an interdisciplinary scientific field drawing from several disciplines such as synthetic biology, genetic engineering, molecular biology, tissue engineering, biochemistry, and food science to design organisms capable of producing a wide variety of agricultural products. (golden.com)
• Harrison pioneered the first methods for the in vitro cultivation of cellular tissues grown outside of animal organisms. (golden.com)

Grown8

• In 1907 Harrison successfully carried out the first tissue culture using the hanging drop method , in which fragments of embryonic frog nerve tissue were grown in a clot of lymphatic fluid. (asu.edu)
• The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. (google.com)
• In animal tissue culture, cells may be grown as two-dimensional monolayers (conventional culture) or within fibrous scaffolds or gels to attain more naturalistic three-dimensional tissue-like structures (3D culture). (wikipedia.org)
• selective culture one grown on a medium, usually solid, that has been supplemented to encourage the growth of a single species of microorganism. (thefreedictionary.com)
• 2. A culture of cells or tissue grown by this technique or process. (thefreedictionary.com)
• In most cases, cells or tissues must be grown in culture for days or weeks to obtain sufficient numbers of cells for analysis. (docplayer.net)
• These three-dimensional cultures are usually grown in bioreactors, small capsules in which the cells can grow into spheroids, or 3D cell colonies. (wikipedia.org)
• Tweezers selecting a round- leaved sundew, Drosera rotundifolia, from several such plants being grown from tissue cultures in a petri dish. (sciencephoto.com)

Sterile7

• For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. (expertsmind.com)
• the maintenance of live tissue after removal from the body, by placing in a vessel with a sterile nutritive medium. (thefreedictionary.com)
• NOTE: All solutions and equipment coming into contact with living cells must be sterile, and aseptic technique should be used accordingly. (docplayer.net)
• 2 Sterile Pasteur pipets 37 C warming tray or incubator Tissue culture plasticware or glassware including pipets and 25-cm 2 flasks or 60-mm petri plates, sterile NOTE: All culture incubations should be performed in a humidified 37 C, 5% CO 2 incubator unless otherwise specified. (docplayer.net)
• DMEM) may require altered levels of CO 2 to maintain ph Remove all medium from primary culture with a sterile Pasteur pipet. (docplayer.net)
• Perform general cell culture procedures more economically with Thermo Scientific™ BioLite Cell Culture Treated Dishes, guaranteed to be sterile, non-pyrogenic and non-cytotoxic. (fishersci.com)
• Accordingly, most culture methods require sterile conditions. (encyclopedia.com)

Growth29

• In serial cultivation fragments of one culture are transferred to new media to form secondary cultures, which ensures that the growth of tissues can be maintained for longer periods. (asu.edu)
• This supplemental extract from embryonic tissues, which he called "embryo juice," proved to be potent in "activating" growth in tissue cultures. (asu.edu)
• He eventually managed to keep the steady growth of embryonic chick heart tissues through serial cultivation for thirty-four years, leading him to conclude that cells were intrinsically immortal. (asu.edu)
• are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth. (expertsmind.com)
• Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. (elsevier.com)
• and growth, differentiation and organogenesis in plant tissue and organ cultures. (elsevier.com)
• Professor Przyborski is developing new and innovative ways to manage the growth and function of cultured cells. (smi-online.co.uk)
• 1. the propagation of microorganisms or of living tissue cells in special media conducive to their growth. (thefreedictionary.com)
• cell culture the maintenance or growth of animal cells in vitro, or a culture of such cells. (thefreedictionary.com)
• continuous flow culture the cultivation of bacteria in a continuous flow of fresh medium to maintain bacterial growth in logarithmic phase. (thefreedictionary.com)
• slant culture one made on the surface of solidified medium in a tube which has been tilted to provide a greater surface area for growth. (thefreedictionary.com)
• Personnel are required to optimise equipment set-up, media and growth techniques. (edu.au)
• They can appear whiteish, yellowish, or black in culture and when in advanced mycelial growth stages look like large fuzzy patches in the dish or flask. (edu.au)
• An important factor influencing the growth of cells in culture is the choice of tissue culture medium. (docplayer.net)
• Secondary cultures are checked for growth, fed periodically, and may be subsequently subcultured to produce tertiary cultures, etc. (docplayer.net)
• Another determining factor of growth and quality in tissue-cultured plants is nutrition. (wur.nl)
• 3D spheroids have been cultured in a lab setting for up to 302 days while still maintaining healthy, non-cancerous growth. (wikipedia.org)
• 9. The method of claim 1 wherein the embryogenic tissue is transferred to semi-solid maintenance medium containing a sufficient amount of nutrients and plant growth hormones under suitable environmental conditions to maintain the embryogenic tissues, prior to transferring the embryogenic tissue to liquid suspension culture maintenance medium. (google.com)
• Growth of human muscle in tissue culture. (cshl.edu)
• The culture technique used readily permitted the growth of both normal and diseased human muscle cells. (cshl.edu)
• Tissue-culture (TC) treated for optimum attachment and growth of anchorage-dependent cells. (fishersci.com)
• Cultures under different concentrations of pectin and manganese chloride (MnCl 2 ) elicitation (i.e., 5 mg/L, 10 mg/L, and 15 mg/L) showed that the control, MnCl 2 10 mg/L, and pectin 15 mg/L suspension extracts greatly inhibited the growth of E. coli , S. typhimurium , and S. aureus (MIC against E. coli , i.e. (hindawi.com)
• A technique for maintenance or growth of animal organs in vitro. (harvard.edu)
• The potential for contamination and bacterial growth at different stages of the culturing process might carry health risks , as might the addition of growth factors and additives, such as hormones, which are used in some conventional meat production outside the EU. (nuffieldbioethics.org)
• Comparative data is not available to indicate whether the concentrations of these hormones and growth factors in cultured meat will be higher, lower or the same as found naturally in animal tissue. (nuffieldbioethics.org)
• Dispersed cells are then transferred to a suitable growth medium and allowed to attach to the surface of culture flasks. (encyclopedia.com)
• Cell culture requires careful attention to the growth medium to ensure cells are given all the components they require to grow. (encyclopedia.com)
• Often the culture medium requires growth factors or hormones to stimulate growth. (encyclopedia.com)
• The general process of cell culture has been used extensively since the early 1900s for research on tissue growth and development, virus biology, properties of cancer cells, studies relating to aging, genetics, and gene therapy. (encyclopedia.com)

Abstract1

• This is a summary of abstract #1780, "Tissue-engineering of Enamel-Dentin Complex Structures", by M. Honda et al. (eurekalert.org)

Differentiation4

• Each technique can be used to investigate a wide spectrum of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to control of proliferation and differentiation. (springer.com)
• We have compared trophoblast viability during perfusion and in explants cultured under various conditions by monitoring glucose consumption, protein synthesis and secretion, expression of differentiation-specific genes, induction of stress proteins and apoptotic cell death. (biomedsearch.com)
• Over 7 h of culture the amount of the differentiation specific placental hormones hCG, hPL and leptin accumulated in the medium dropped significantly. (biomedsearch.com)
• Further, we demonstrate for the first time a volumetric quantification of endogenous biomolecules using 3D Raman imaging datasets that allows us to spatially monitor complex biological processes like differentiation within a 3D cell culture system. (nature.com)

Viability4

• Trophoblast viability in perfused term placental tissue and explant cultures limited to 7-24 hours. (biomedsearch.com)
• We observed a severe loss of trophoblast viability in explants irrespective of the culture conditions used. (biomedsearch.com)
• This study investigates the effect of different storage temperatures on the viability, morphology, and phenotype of cultured RPE. (hindawi.com)
• Furthermore, the chondrocytes should be implanted after two weeks of culture, when the highest viability rate is found. (scielo.br)

Explants2

• Primary cultures may be derived from blood or tissue explants and thousands of immortalized cell lines are now available from tissue culture collections. (selectscience.net)
• The term tissue culture arose because most of the early cells were derived from primary tissue explants, a technique that dominated the field for over 50 years. (fsu.edu)

Endothelial Cells2

• Culture of Human Brain Tumors on an Extracellular Matrix Derived from Bovine Corneal Endothelial Cells and Cultured Human Glioma Cells. (barnesandnoble.com)
• MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells. (harvard.edu)

Cell and Tissue Culture Supplies4

• This report analyzes the worldwide markets for Cell and Tissue Culture Supplies in US$ Thousands by the following Product Segments: Media, Sera, and Reagents. (marketpublishers.com)
• Who Are Your Competitors in the Cell and Tissue Culture Supplies Market? (strategyr.com)
• Amid the growing push for decoupling and economic distancing, the changing relationship between China and the rest of the world will influence competition and opportunities in the Cell and Tissue Culture Supplies market. (strategyr.com)
• Continuous monitoring for emerging signs of a possible new world order post-COVID-19 crisis is a must for aspiring businesses and their astute leaders seeking to find success in the now changing Cell and Tissue Culture Supplies market landscape. (strategyr.com)

Proteins3

• These results are in contrast to the findings obtained in perfused placental tissue where, after 7 h of culture, hormone secretion, expression of stress proteins and cell death were similar as in native tissue. (biomedsearch.com)
• d) repeating steps (b) and (c) as necessary to produce extracellular matrix proteins in the three dimensional stromal culture. (google.com)
• Specifically outlined are methods for collecting and treating tissue to obtain (i) DNA for high molecular weight genomic analyses, (ii) RNA for tissue-specific transcriptomes, and (iii) proteins for proteomic-level analyses. (jove.com)

Genes2

• Expression of Foreign Genes in Cultured Insect Cells Using a Recombinant Baculovirus Vector. (barnesandnoble.com)
• Another difficulty with some cultured cells is their tendency to change their morphology , functions, or the range of genes they express. (encyclopedia.com)

Fragments7

• Conceptually, they coined the term "tissue culture" and gave its definition as "a plasmatic medium inoculated with small fragments of living tissue. (asu.edu)
• In 1913, E. Steinhardt, C. Israeli, and R. A. Lambert grew vaccinia virus in fragments of guinea pig corneal tissue. (wikipedia.org)
• Tissue fragments were incubated for 60 minutes with one of the viral vectors and transferred to fresh medium. (curehunter.com)
• At 3 days, beta-gal expression was observed in 33% of tissue fragments exposed to the vaccinia vector and in 18% and 14% of fragments exposed to the adenoviral and herpes vectors, respectively. (curehunter.com)
• Researchers in the labs of the Rockefeller Institute, most notably Dr.Raymond C. Parker, designed and described many new systems for culturing large numbers of tissue fragments. (golden.com)
• a reservoir chamber that holds the culture medium and a culture chamber that holds tissue fragments. (golden.com)
• These techniques and culturing systems developed by scientists at the Rockefeller Institute for medical research allowed scientists to begin culturing tissue fragments in large quantities for the first time using a single flask system that grows tissues on a thin layer of nutritional medium with sufficient and constantly circulating oxygen. (golden.com)

Micropropagation2

• It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). (worldcat.org)
• Another major application of plant tissue culture is micropropagation: vegetative propagation in vitro. (wur.nl)

Bioreactor3

• Approximately 300 spheroids are usually cultured per bioreactor. (wikipedia.org)
• The process is housed within a closed bioreactor to provide sterility and an optimal environment for cells to be cultured. (nuffieldbioethics.org)
• First known bioreactor for tissue culturing made by Ross Harrison in 1912. (golden.com)

Biology5

• Tissue culture is an important technique which is used to study the fundamental problems in cell biology. (expertsmind.com)
• Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. (elsevier.com)
• He has over 25 years experience in cell biology with specific interests in cell culture technology, tissue engineering and stem cell research. (smi-online.co.uk)
• Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
• For the last forty years, biologists have used traditional 2D cell culture to forge breakthroughs in cell biology. (labx.com)

Organoids5

• The culture of organoids has permitted researchers access to a highly physiologically relevant system for studying human disease. (corning.com)
• While their value is undeniable, culturing organoids can be quite challenging. (corning.com)
• Culturing organoids is a likewise sensitive process and the specific requirements of the cells and the organoids needs to be evaluated. (corning.com)
• The trending advent of cardiovascular organoids further elevates the premises of 3D culture in a better understanding of heart development and congenital heart defects. (frontiersin.org)
• To address this challenge, we have developed an assortment of bioengineered tissue organoids and tissue constructs that are integrated in a closed circulatory perfusion system, facilitating inter-organ responses. (nature.com)

Callus cultures2

• Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. (routledge.com)
• Welsh from 6-month-old callus cultures. (springer.com)

Dissociation1

• Sreedevi, S. 2016-06-29 00:00:00 Explantation and trypsinisation methods for tissue dissociation were attempted for the establishment of primary cell cultures from the caerulean damsel, Pomacentrus caeruleus. (deepdyve.com)

PERFUSION3

• A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. (umassmed.edu)
• Human term-placental culture techniques such as villous explant or dual perfusion are commonly used to study trophoblast function under control and experimentally manipulated conditions. (biomedsearch.com)
• This difference between villous explant incubation and dual perfusion is also reflected by a significantly higher consumption of glucose in perfused tissue. (biomedsearch.com)

Spheroids3

• In this study, to generate and culture multiple 3D cell spheroids, we exploited laser ablation and replica molding for the fabrication of polydimethylsiloxane (PDMS) multi-well chips, which were validated using articular chondrocytes (ACs). (nih.gov)
• In conclusion, multi-well chips for the generation and culture of multiple cell spheroids can be fabricated by low-cost rapid prototyping techniques. (nih.gov)
• Multicellular spheroids in this aspect are ideal building units for tissue reconstruction. (wiley.com)

Protocols6

• The Tissue Culture Tech I position will be part of a team to maintain and multiple current in-house tissue culture material as well as work on improving existing cannabis tissue culture protocols. (biotalent.ca)
• It includes protocols for bat capture and dissection, tissue preservation, and cell culturing of bat tissue. (jove.com)
• A series of protocols to optimize tissue collection from bats has been developed for a series of high-throughput sequencing approaches. (jove.com)
• Outlined here are protocols for the capture of bats, desired demographics to be collected for each bat, and optimized methods to minimize stress on a bat during tissue collection. (jove.com)
• The protocols outlined herein define the ideal tissue collection and tissue preservation methods for Bat1K, the consortium that is sequencing the genomes of every species of bat. (jove.com)
• In addition to genomic data, functional understanding of the diversity of bat adaptations requires tissue-specific transcriptome and protein analyses, often requiring separate collection protocols. (jove.com)

Contamination4

• Cell cultures can often be cured of fungus contamination when detected early by treatment with certain antibiotics (actually antimycotics). (edu.au)
• seasonal changes of fall and spring usually result in an increase in this type of contamination in cultures as heating or A/C systems are switched on or off. (edu.au)
• Maintenance of cells in long-term culture requires strict adherence to aseptic technique to avoid contamination and potential loss of valuable cell lines (see UNIT 1.3). (docplayer.net)
• The limitations of cell culture include the finite doubling potential of most normal cells, the possibilities for unexpected infection with viruses or microorganisms, or even cross-contamination with other cell types. (encyclopedia.com)

Organs3

• Retrieved May 15, 2011 from (http://www.ted.com/talks/anthony_atala_growing_organs_engineering_tissue). (wikipedia.org)
• One reason for such poor translation from drug candidate to successful use is a lack of model systems that accurately recapitulate normal tissue function of human organs and their response to drug compounds. (nature.com)
• A central advantage of the cell culture technique is its simplicity compared to the difficulties of studies using whole plant or animal organs, which are usually composed of many different cell types. (encyclopedia.com)

Optimization1

• This study enumerates the antibacterial and antioxidant potentials of N. sativa epicotyl suspension cultures under biotic and abiotic elicitation along with concentration optimization of the elicitors for enhanced TQ and THY production. (hindawi.com)

Suspension cultures3

• Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. (springer.com)
• the Alternate Protocol 1 describes passaging of suspension cultures. (docplayer.net)
• In particular, this invention relates to an improved method for establishing and maintaining embryogenic liquid suspension cultures for use in somatic embryogenesis processes from plants of the genus Pinus and Pinus interspecies. (google.com)

Beef burger1

• A prototype of a cultured beef burger, funded by Google co-founder Sergey Brin, was announced in 2013. (nuffieldbioethics.org)

Propagation4

• Anderson WC (1980) Mass propagation by tissue culture. (springer.com)
• Debergh PC, Maene LJ (1981) A scheme for commercial propagation of ornamental plants by tissue culture. (springer.com)
• In: George EF, Hall MA, De Klerk GJ (eds) Plant propagation by tissue culture. (springer.com)
• Tissue Culturing & Other Propagation Techniques of Banana Plants This forum is for discussing propagation techniques of banana plants. (bananas.org)

Protoplast2

• Duquenne B, Eeckhaut T, Werbrouck S, Van Huylenbroeck J (2007) Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium . (springer.com)
• Plant tissue culture is an essential component of many present-day breeding techniques, such as anther culture, protoplast technology, embryo rescue and microsporogenesis. (wur.nl)

Embryo5

• Traditional plant breeding is enhanced with techniques such as embryo culture to rescue recalcitrant hybrids. (springer.com)
• Bridgen MP (1994a) A review of plant embryo culture. (springer.com)
• Another important modification was the addition of embryo extracts to the culture medium . (asu.edu)
• The "embryo juice" was made by grinding up embryonic tissues, mixing them with saline solution, and eliminating remaining cells through centrifugation and filtration. (asu.edu)
• Among many speculations proposed to account for how Carrel's chick heart tissue could have survived in tissue culture for so long, was the question of whether the "embryo juice" was in fact introducing extra living cells that had not been eliminated by the centrifugation and filtration processes. (asu.edu)

Isolation2

• Moreover, tissues in the body do not exist in isolation, but reside in a highly integrated and dynamically interactive environment, in which actions in one tissue can affect other downstream tissues. (nature.com)
• Defined, xeno-free, serum-free culture medium, Designed to support the isolation and expansion of human mesenchymal stem cells (MSCs). (selectscience.net)

Burrows7

• Burrows successfully established tissue cultures of embryonic chick cells. (asu.edu)
• Between October 1910 and March 1911, Carrel and Burrows published four papers on tissue culture in the Journal of the American Medical Association . (asu.edu)
• Experimentally, Carrel and Burrows adapted Harrison's hanging drop method of amphibian embryonic cells for wider applications and so that tissues could be cultured for a longer duration. (asu.edu)
• Although the method they used was generally based on Harrison's original design, Carrel and Burrows substituted blood plasma for lymph in the tissue culture medium . (asu.edu)
• Carrel and Burrows called this prolonged culturing "reactivation and cultivation in series. (asu.edu)
• The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. (wikipedia.org)
• Plant tissue culture in particular is concerned with the growing of entire plants from small pieces of plant tissue, cultured in medium Cell culture Organ culture Plant tissue culture Carrel, Alexis and Montrose T. Burrows (1911). (wikipedia.org)

Somatic1

• Agache S, de Buyser J, Henry Y, Snape JW (1988) Studies on the genetic relationship between anther culture and somatic tissue culture abilities in wheat. (springer.com)

Carrel4

• Alexis Carrel , the prominent French surgeon, biologist, and 1912 Nobel Prize laureate for Physiology or Medicine, was one of the pioneers in developing and modifying tissue culture techniques. (asu.edu)
• The publicized work of Carrel and his associates at the Rockefeller Institute established the practice of long-term tissue culture for a wide variety of cells. (asu.edu)
• Although the serial culturing technique could extend the life span of the cultures, Carrel noticed that the size of the cells decreased with each subculture. (asu.edu)
• With the aid of this extract Carrel was able to grow tissues for months. (asu.edu)

Embryonic5

• In 1913 he found that cell size could be maintained by adding extracts of homogenized tissues from chicks, especially of embryonic origin. (asu.edu)
• 1. No multiplication of acid-fast bacteria has been observed in our cultures of rat leprosy in minced embryonic (foetal or neoplastic) tissues suspended in Tyrode solution. (ajtmh.org)
• In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
• Embryonic Rodent Brain Cells in Culture. (barnesandnoble.com)
• Derivation and Maintenance of Embryonic Stem Cell Cultures. (barnesandnoble.com)

Flask2

• If this is the first medium change, rather than discarding medium that is removed from primary culture, put it into a fresh dish or flask. (docplayer.net)
• Single flask tissue culturing apparatus with two separate chambers connected to a gas line. (golden.com)

Preparation2

• Transmission and Scanning Electron Microscope Preparation of Whole Cultured Cells. (barnesandnoble.com)
• This is a protocol for the optimal tissue preparation for genomic, transcriptomic, and proteomic analyses of bats caught in the wild. (jove.com)

Primary11

• Cultures prepared frorn cells which were removed from primary cultures form sc~odalryc ultures. (expertsmind.com)
• Primary Cultures of Rat Hepaytes. (barnesandnoble.com)
• primary culture a cell or tissue culture made by direct transfer from a natural source to an artificial medium. (thefreedictionary.com)
• Gene delivery by viral vectors in primary cultures of lacrimal gland tissue. (curehunter.com)
• To test the feasibility of gene transfer into lacrimal gland tissue in primary culture, using different viral vectors. (curehunter.com)
• Tissue was maintained under primary organ culture conditions using the "raft" technique. (curehunter.com)
• Vaccinia and adenovirus are efficient vectors for gene transfer into lacrimal gland tissue in primary culture. (curehunter.com)
• The first step in subculturing monolayers is to detach cells from the surface of the primary culture vessel by trypsinization or mechanical means. (docplayer.net)
• The process of removing cells from the primary culture and transferring them to secondary cultures constitutes a passage, or subculture. (docplayer.net)
• Lastly, also outlined is a method to avoid lethal sampling by creating viable primary cell cultures from wing clips. (jove.com)
• In addition, the culture was immunofluorescently labeled with Alexa Fluor 568 conjugated to goat secondary antibodies that target mouse anti-PDI (protein disulfide isomerase) primary antibodies (red fluorescence). (fsu.edu)

Extracellular matrix2

• The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue. (google.com)
• This work focused on the importance of the extracellular matrix and the ability of cultures in artificial 3D matrices to produce physiologically relevant multicellular structures, such as acinar structures in healthy and cancerous breast tissue models. (wikipedia.org)

Phenotype1

• TGF-beta3 maintains the phenotype of disc cells in organ culture. (nih.gov)

Establishment2

• The establishment of future retinal pigment epithelium (RPE) replacement therapy is partly dependent on the availability of tissue-engineered RPE cells, which may be enhanced by the development of suitable storage methods for RPE. (hindawi.com)
• This work evaluated the effect of freezing on chondrocytes maintained in culture, aiming the establishment of a cell bank for future application as heterologous implant. (scielo.br)

Hormones1

• British biologist who developed ways to grow cells outside the body ("tissue culture") in order to more closely study the cells and the effects of hormones, vitamins, and other chemicals. (encyclopedia.com)

20011

• do Valle Rego L, Tadeu de Faria R (2001) Tissue culture in ornamental plant breeding: a review. (springer.com)