Culture Techniques
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Organ Culture Techniques
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Tissue Culture Techniques
Bacteria
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Evaluation Studies as Topic
Blood
Batch Cell Culture Techniques
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Colony Count, Microbial
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Cells, Cultured
Sensitivity and Specificity
Feces
Pharynx
A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).
Agar
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Centrifugation
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Bacteroides
Fungi
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Specimen Handling
Reagent Kits, Diagnostic
Sputum
Anaerobiosis
Sepsis
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
Cell Differentiation
Hemolysis
Staphylococcus
A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
Colony-Forming Units Assay
RNA, Ribosomal, 16S
Water Microbiology
Tissue Engineering
DNA, Ribosomal
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Coculture Techniques
Stem Cells
Cattle
Mycobacterium tuberculosis
Immunoenzyme Techniques
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Cell Division
Species Specificity
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
DNA Primers
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Bone Marrow Cells
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
Rabbits
Embryo Culture Techniques
Studies on the response of ewes to live chlamydiae adapted to chicken embryos or tissue culture. (1/6081)
Ewes infected before gestation with chicken embryo or tissue culture adapted chlamydial strain B-577 were challenge inoculated with the homologous strain at four to 18 weeks of gestation. The ewes responsed with group specific complement fixing antibody titers of 1:8 to 1:256 by the second week after initial infection. A secondary antibody response in the surviving challenge inoculated ewes occurred at the time of lambing and reached titers of 1:32 to 1:256 by the second week after parturition. Group specific complement fixing antibodies did not appear to play a significant role in resistance to chlamydial infection. Ewes infected with the chicken embryo adapted strain B-577 excreted chlamydiae in their feces 60 days after inoculation. However, chlamydiae were not recovered from feces of ewes infected with the tissue culture adapted strain B-577. Placentas of ewes challenge inoculated by the intravenous route were consistently infected. Chlamydiae were recovered from placentas, some fetuses and lambs. In two instances when challenge inoculation was given by the intramuscular route, infection was detected only by the direct fluorescent antibody method. (+info)In vitro development of sheep preantral follicles. (2/6081)
Preantral ovarian follicles isolated from prepubertal sheep ovaries were individually cultured for 6 days in the presence of increasing doses of FSH (ranging from 0.01 to 1 microg/ml) and under two different oxygen concentrations, 20% and 5% O2. Follicle development was evaluated on the basis of antral cavity formation as well as the presence of healthy cumulus oocyte complexes. Follicle growth was enhanced by FSH addition to culture medium, while the use of a low oxygen concentration slightly stimulated this process. However, when follicles were cultured in the presence of high doses of FSH (1 microgram/ml) and under low oxygen concentration, a high proportion of them showed the presence of an antral cavity and of a healthy cumulus-oocyte complex. In addition, under this specific culture condition sheep preantral follicles released higher levels of estradiol as compared to those secreted at lower FSH concentrations or under 20% O2. When the meiotic competence of oocytes derived from follicles cultured at 1 microgram/ml FSH was assessed, no significant difference was recorded between the two oxygen groups. These results show that the culture conditions here identified are beneficial to in vitro growth and differentiation of sheep preantral follicles. (+info)Ontogeny of expression of a receptor for platelet-activating factor in mouse preimplantation embryos and the effects of fertilization and culture in vitro on its expression. (3/6081)
Platelet-activating factor (PAF; 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent ether phospholipid. It is one of the preimplantation embryo's autocrine growth/survival factors. It may act via a G protein-linked receptor on the embryo; however, the evidence for this is conflicting. The recent description of the intracellular form of the PAF:acetlyhydrolase enzyme as having structural homology with G proteins and Ras also suggests this as a potential intracellular receptor/transducer for PAF. This study used reverse transcription-polymerase chain reaction to examine the ontogeny of expression of the genes for these proteins in the oocyte and preimplantation-stage embryo. Transcripts for the G protein-linked PAF receptor were detected in the late 2-cell-stage embryo and in all stages from the 4-cell stage to blastocysts. They were also present in unfertilized oocytes and newly fertilized zygotes but only at relatively low levels. The incidence of expression was generally low and variable in late zygotes and early 2-cell embryos. Expression past the 2-cell stage was alpha-amanitin sensitive. The results indicated that mRNA for this receptor is a maternal transcript that was degraded during the zygote-2-cell stage. New expression of the receptor transcript required activation of the zygotic genome. Fertilization of embryos in vitro caused this transcript not to be expressed in the zygote. Culture of zygotes (irrespective of their method of fertilization) caused expression from the zygotic genome to be retarded by more than 24 h. This retardation did not occur if culture commenced at the 2-cell stage. The transcripts for the subunits of intracellular PAF:acetylhydrolase were not detected in oocytes or at any stage of embryo development examined, despite their being readily detected in control tissue. This study confirms the presence of the G protein-linked PAF receptor in the 2-cell embryo and describes for the first time its normal pattern of expression during early development. The adverse effects of in vitro fertilization (IVF) and embryo culture on the expression of this transcript may be a contributing factor for the poor viability of embryos produced in this manner. The reduced expression of PAF-receptor mRNA following IVF predicts that such embryos may have a deficiency in autocrine stimulation and also suggests that supplementation of growth media with exogenous PAF would be only partially beneficial. The effect of IVF and culture may also explain the conflicting literature. (+info)Steroid regulation of retinol-binding protein in the ovine oviduct. (4/6081)
Two studies were conducted to identify retinol-binding protein (RBP) expression in the ovine oviduct and to determine the role of ovarian steroids in its regulation. Ewes were salpingectomized on Days 1, 5, or 10 of their respective estrous cycles, and oviducts were homogenized for RNA analysis, fixed for immunocytochemistry (ICC), or cultured for 24 h for protein analysis. ICC localized RBP to the epithelium of all oviducts. RBP synthesis was demonstrated by immunoprecipitation of radiolabeled RBP from the medium of oviductal explant cultures. Explant culture medium from oviducts harvested on Day 1 contained significantly more RBP than medium from oviducts collected on Days 5 or 10. Slot-blot analysis demonstrated that steady-state RBP mRNA levels were significantly higher on Day 1 than Day 5 or 10. In the second experiment, ovariectomized ewes were treated with estradiol-17beta (E2), progesterone (P4), E2+P4 (E2+P4), or vehicle control, and oviducts were analyzed as above. P4 alone or in combination with E2 significantly reduced steady-state RBP mRNA levels compared to those in E2-treated animals. Oviductal explants from E2- and E2+P4-treated animals released 3- to 5-fold more RBP into the medium than control and P4 treatments as determined by ELISA. RBP synthesis of metabolically labeled RBP was increased by E2 and E2+P4 treatments. This study demonstrates that P4 applied on an estradiol background negatively regulates RBP gene expression in the oviduct whereas estradiol appears to stimulate RBP synthesis and secretion. (+info)Onset of nucleolar and extranucleolar transcription and expression of fibrillarin in macaque embryos developing in vitro. (5/6081)
Specific aims were to characterize the onset of nucleolar and extranucleolar transcription and expression of the nucleolar protein fibrillarin during preimplantation development in vitro in macaque embryos using autoradiographic and immunocytochemical techniques. Autoradiography was performed on whole embryos cultured with [3H]uridine for assessment of nucleolar (rRNA) and extranucleolar (mRNA) transcription. Expression of fibrillarin was immunocytochemically assessed in whole embryos using a primary antibody against fibrillarin and a fluorescein isothiocyanate-conjugated secondary antibody. Extranucleolar incorporation of [3H]uridine was first detected in 2-cell embryos cultured 6-10 h with [3H]uridine. Culture with alpha-amanitin prevented incorporation of label in 2-cell embryos, and treatment with ribonuclease reduced the signal to background levels, indicating that [3H]uridine was incorporated into mRNA and not rRNA or DNA. Nucleolar incorporation of [3H]uridine was not evident in pronucleate-stage or 2- to 5-cell embryos, but it was detected in one 6-cell embryo and in all 8-cell to blastocyst-stage embryos. Fibrillarin was first expressed in some 6- to 7-cell embryos, but it was consistently expressed in all 8-cell embryos. Fibrillarin was localized to the perimeter of the nucleolar precursor bodies, forming a ring that completely encapsulated these structures. Fibrillarin was not expressed in 8- to 16-cell embryos cultured with alpha-amanitin, indicating that it is transcribed, rather than recruited, at the 8-cell stage. In conclusion, in in vitro-fertilized macaque embryos developing in vitro, extranucleolar synthesis of mRNA is initiated at the 2-cell stage while the onset of nucleolar transcription occurs at the 6- to 8-cell stage, coincident with expression of fibrillarin. (+info)Investigation of distal aortic compliance and vasodilator responsiveness in heart failure due to proximal aortic stenosis in the guinea pig. (6/6081)
Hypotension and syncope are recognized features of chronic aortic stenosis. This study examined vasomotor responses and dynamic compliance in isolated abdominal aortae after chronic constriction of the ascending aorta. Guinea pigs underwent constriction of the ascending aorta or sham operation. Sections of descending aorta were removed for studies of contractile performance and compliance. Dynamic compliance was measured using a feedback-controlled pulsatile pressure system at frequencies of 0.5, 1.5 and 2.5 Hz and mean pressures from 40 to 100 mmHg. Chronic (149+/-6 days) aortic constriction resulted in significant increases in organ weight/body weight ratios for left ventricle (58%), right ventricle (100%) and lung (61%). The presence of heart failure was indicated by increased lung weights, left ventricular end-diastolic pressure and systemic vascular resistance, reduced cardiac output and increased levels of plasma atrial natriuretic peptide (166%), adrenaline (x20), noradrenaline (106%) and dopamine (x3). Aortic rings showed similar constrictor responses to phenylephrine and angiotensin II, but maximal vasodilator responses to acetylcholine and isoprenaline were significantly increased (144% and 48% respectively). Dilator responses to sodium nitroprusside, forskolin and cromokalim were unchanged. Compliance of all vessels decreased with increasing pulsatile frequency and to a lesser extent with increased mean pressure, but were similar in aortic-constricted and control groups. Chronic constriction of the ascending aorta resulted in heart failure and increased vasodilator responses to acetylcholine and isoprenaline in the distal aorta while dynamic compliance was unchanged. We hypothesize that increased endothelium-mediated vasodilatation may contribute to hypotension and syncope in patients with left ventricular outflow obstruction. (+info)Characterization of beta cells developed in vitro from rat embryonic pancreatic epithelium. (7/6081)
The present study evaluates the development and functional properties of beta cells differentiated in vitro. The authors have previously demonstrated that when E12.5 rat pancreatic rudiments are cultured in vitro in the absence of mesenchyme, the majority of the epithelial cells differentiate into endocrine beta cells. Thus, depletion of the mesenchyme provokes the expansion of endocrine tissue at the expense of exocrine tissue. The potential use of this procedure for the production of beta cells led the authors to characterize the beta cells differentiated in this model and to compare their properties with those of the endocrine cells of the embryonic and adult pancreas. This study shows that the beta cells that differentiate in vitro in the absence of mesenchyme express the homeodomain protein Nkx6.1, a transcription factor that is characteristic of adult mature beta cells. Further, electron microscopy analysis shows that these beta cells are highly granulated, and the ultrastructural analysis of the granules shows that they are characteristic of mature beta cells. The maturity of these granules was confirmed by a double-immunofluorescence study that demonstrated that Rab3A and SNAP-25, two proteins associated with the secretory pathway of insulin, are strongly expressed. Finally, the maturity of the differentiated beta cells in this model was confirmed when the cells responded to stimulation with 16 mM glucose by a 5-fold increase in insulin release. The authors conclude that the beta cells differentiated in vitro from rat embryonic pancreatic rudiments devoid of mesenchyme are mature beta cells. (+info)Long-term effects of prior heat shock on neuronal potassium currents recorded in a novel insect ganglion slice preparation. (8/6081)
Brief exposure to high temperatures (heat shock) induces long-lasting adaptive changes in the molecular biology of protein interactions and behavior of poikilotherms. However, little is known about heat shock effects on neuronal properties. To investigate how heat shock affects neuronal properties we developed an insect ganglion slice from locusts. The functional integrity of neuronal circuits in slices was demonstrated by recordings from rhythmically active respiratory neurons and by the ability to induce rhythmic population activity with octopamine. Under these "functional" in vitro conditions we recorded outward potassium currents from neurons of the ventral midline of the A1 metathoracic neuromere. In control neurons, voltage steps to 40 mV from a holding potential of -60 mV evoked in control neurons potassium currents with a peak current of 10.0 +/- 2.5 nA and a large steady state current of 8.5 +/- 2.6 nA, which was still activated from a holding potential of -40 mV. After heat shock most of the outward current inactivated rapidly (peak amplitude: 8.4 +/- 2.4 nA; steady state: 3.6 +/- 2.0 nA). This current was inactivated at a holding potential of -40 mV. The response to temperature changes was also significantly different. After changing the temperature from 38 to 42 degrees C the amplitude of the peak and steady-state current was significantly lower in neurons obtained from heat-shocked animals than those obtained from controls. Our study indicates that not only heat shock can alter neuronal properties, but also that it is possible to investigate ion currents in insect ganglion slices. (+info)
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Traditional Techniques
"Stephen Malkmus: Traditional Techniques". Spectrum Culture. Archived from the original on October 31, 2020. Retrieved March 6, ... "BEATINK.COM Traditional Techniques". Beatink.com. Retrieved January 11, 2021. "Stephen Malkmus Traditional Techniques". ... Traditional Techniques is a solo album by American musician Stephen Malkmus, the fourth album credited to Malkmus and the ... Traditional Techniques received positive reviews from music critics. Many critics praised Malkmus' foray into folk music. At ...
Transcendental Meditation technique
... whose goal is to create a better culture." Charles H. Lippy writes that earlier spiritual interest in the technique faded in ... Other initiatives to teach the TM technique to war veterans at risk for PTSD, were underway as of 2010. The technique has been ... The Transcendental Meditation technique (abbreviated as TM) is the technique associated with the practice of Transcendental ... According to the Maharishi his technique requires no preparation, is simple to do, and can be learned by anyone. The technique ...
Chinese art by medium and technique
2006). Aspect of Chinese culture. 中国文化导读. 清华大学出版社 publishing Institute of the History of Natural Sciences and Chinese Academy ... The earliest extant lacquer object, a red wooden bowl, was unearthed at a Hemudu culture (c. 5000-4500 BCE) site. By the Han ... when the sophisticated techniques used in the lacquer process were first developed, and it became a highly artistic craft. ...
Genetic engineering techniques
One of the simplest methods involves using calcium phosphate to bind the DNA and then exposing it to cultured cells. The ... Genetic engineering techniques allow the modification of animal and plant genomes. Techniques have been devised to insert, ... If successful, the technique produces an adult plant that contains the transgene in every cell. In animals it is necessary to ... The DNA band at the correct size should contain the gene, where it can be excised from the gel.: 40-41 Another technique to ...
Propaganda techniques
The Culture of the New Capitalism. Yale University Press. p. 172. A Citizens Guide to Understanding Corporate Media Propaganda ... This technique is used to convince the audience that a program is an expression of an irresistible mass movement and that it is ... This technique has also been referred to as the PT Barnum effect. (e.g., the advertising campaign slogan "Ford has a better ... Semantic Satiation This technique can be used with the aim of lessening the impact of a damaging headline or sound byte. For ...
Graham technique
ISBN 978-1-134-35821-2. Daly, Ann (2002). Critical Gestures: Writings on Dance and Culture. Wesleyan University Press. p. 158. ... It is widely regarded as the first codified modern dance technique, and strongly influenced the later techniques of Merce ... Modern dance Humphrey-Weidman technique Limón technique Excerpts from Guidelines in Contemporary Dance Training, a DVD set ... her extended collaboration with ballet-trained Erick Hawkins made her technique more balletic over time.: 23 The technique was ...
Early Japanese iron-working techniques
P.19 Technology and the culture of progress in meiji japan. P.24 Technology and the culture of progress in meiji japan. P.24 ... Technology and the culture of progress in meiji japan. P.25 Technology and evolution: a root and branch view of asian iron from ... However, the best archaeological evidence for early iron-working techniques in Japan dates to the Asuka period, after Buddhism ... Early Japanese iron-working techniques are known primarily from archaeological evidence dating to the Asuka period (538-710 CE ...
Filmmaking technique of Luis Buñuel
D'Lugo, Marvin (1999). "Buñuel in the Cathedral of Culture" in Marsha Kinder (ed.), Luis Buñuel's The Discreet Charm of the ... Buñuel's filmmaking technique was influenced by many aspects of his personality which included sharp contrasts of character and ... Often Buñuel applies the techniques of mise-en-scène to combine multiple single scenes within a film directed by him to ... 4 in E Minor, a practice called by James Clifford "fortuitous or ironic collage." Although Buñuel's use of this technique ...
Levallois technique
... has shown that processes signifying the end of the Acheulian culture and the emergence of a Middle Palaeolithic culture ... The Levallois technique (IPA: [lə.va.lwa]) is a name given by archaeologists to a distinctive type of stone knapping developed ... Yubetsu technique Higham, Tom (2021). The World Before Us: How Science is Revealing a New Story of Our Human Origins. Penguin ... The technique was more sophisticated than earlier methods of lithic reduction, involving the striking of lithic flakes from a ...
Agricultural machinery
Les outils agricoles à l'épreuve de l'open source". Techniques & Culture. Revue semestrielle d'anthropologie des techniques (in ...
Wi-Fi
Decker, Kris De (6 June 2017). "Comment bâtir un internet low tech". Techniques & Culture. Revue semestrielle d'anthropologie ... Different versions of Wi-Fi use different techniques, 802.11b uses DSSS on a single carrier, whereas 802.11a, Wi-Fi 4, 5 and 6 ... Mateti, Prabhaker (2005). "Hacking Techniques in Wireless Networks". Dayton, Ohio: Wright State University Department of ... or 4G networks using the tethering technique. Many smartphones have a built-in capability of this sort, including those based ...
Mail art
Artistic techniques, Contemporary art movements, DIY culture, Fluxus, Postmodern art, Cassette culture 1970s-1990s). ... Certain materials and techniques are commonly used and frequently favored by mail artists due to their availability, ... Various printmaking techniques, in addition to rubber stamping, are used to create multiples. Copy art (xerography, photocopy) ... Mail artists often use collage techniques to produce original postcards, envelopes and work that may be transformed using copy ...
Obi (sash)
The technique nishijin-ori, traditionally produced in the Nishijin area of Kyoto, is intricately woven and can have a three ... ISBN 0-88011-598-X. Dalby, Liza (2001). Kimono, Fashioning Culture. Vintage. ISBN 0-09-942899-7. Fält, Olavi K.; Nieminen, Kai ... Bennett, Gary (1997). Aikido techniques & tactics. Human Kinetics Publisher. ... dye technique. Obidome (帯留, "sash clip") is a small, decorative brooch fastened onto the obijime at the front, commonly made ...
Dada
Cut-Up Technique , Open Culture". Retrieved 2021-10-31. Marc Lowenthal, translator's introduction to Francis Picabia's I Am a ... Cut-up technique is an extension of collage to words themselves, Tristan Tzara describes this in the Dada Manifesto: TO MAKE A ... Dada Culture (New York and Amsterdam: Rodopi Verlag, 2006) Lavin, Maud. Cut With the Kitchen Knife: The Weimar Photomontages of ... Europe of Cultures. "Tristan Tzara speaks of the Dada Movement" Archived 2015-07-04 at the Wayback Machine, September 6, 1963. ...
Malangbang
Techniques & Culture [Online]. Nugroho, Irawan Djoko (2011). Majapahit Peradaban Maritim. Suluh Nuswantara Bakti. ISBN 978-602- ...
Germanium dioxide
Robert Arthur Andersen (2005). Algal culturing techniques. Elsevier Academic Press. ISBN 9780120884261. Tao, S. H.; Bolger, P. ... For this application, the concentration of germanium dioxide typically used in the culture medium is between 1 and 10 mg/L, ... Germanium dioxide is used in algaculture as an inhibitor of unwanted diatom growth in algal cultures, since contamination with ...
Hiroshi Tamiya
"Historical review of algal culturing techniques". In Anderson, Robert A. (ed.). Algal Culturing Techniques. Elsevier Science & ... Tamiya was able with it to culture algal cell lines that were all in the same developmental stage, a technique used by later ... In 1953 Tamiya, working with other Japanese scientists, developed techniques for the synchronous culture of the green algae ... In 1977 he was given the Japanese Order of Culture for his contributions to science in Japan. The standard author abbreviation ...
Yehuda Yannay
ISBN 978-0-02-872100-2. Rubin, Emanuel; Baron, John H. (2006). Music in Jewish History and Culture. Sterling Heights, Michigan ... Boskovich introduced him immediately to twentieth-century techniques and influenced him greatly as a composer and, eventually, ... ISBN 0-674-37471-1. Read, Gardner (1976). Contemporary Instrumental Techniques. New York: Schirmer. ... extended and invented vocal and instrumental techniques, and required virtuosity and perseverance from performers. In fact, ...
Arthur Asa Berger
1982). Media analysis techniques. Beverly Hills: Sage Publications. (1984). Signs in contemporary culture: An introduction to ... 2011). Ads, Fads, and Consumer Culture: Advertising's Impact on American Character and Society. Boulder, Colo: Rowman & ... Walnut Creek, California: AltaMira Press (1998). Media research techniques. Thousand Oaks: Sage Publications. (2003). Durkheim ... Asa Berger Professor Emeritus Broadcast and Electronic Communication Arts San Francisco State University.The Popular Culture ...
Denis Vidal
Techniques et culture, 1 (67), Éditions de l'EHESS, 288 p., 2017. Vidal, Denis; Meyer, Eric; Tarabout, Gilles, eds. (1994). ... 2017). "67 , 2017 Low Tech ? Wild Tech !". Techniques & Culture (67). doi:10.4000/tc.8260. Grimaud, Emmanuel; Tastevin, Yann ... 2017). "67 , 2017 Low Tech ? Wild Tech !". Techniques & Culture (67). doi:10.4000/tc.8260. "Persona, étrangement humain , Actes ... Techniques et Culture , 2017 Vidal, Denis (2012). "Vers un Nouveau Pacte Anthropomorphique! Les Enjeux Anthropologiques de la ...
Opus reticulatum
... the technique had rare appearances in Africa forming another facing called opus africanum.: 189-90 The technique used vertical ... Van Oyen, Astrid (2017). "Finding the Material in 'Material Culture'. Form and Matter in Roman Concrete.". In Van Oyen, Astrid ... Along with other facings, the technique is also important for dating buildings in modern scholarship where there is an absence ... The chronology of the usage of facings is fluid with techniques being used long after their popularity has waned.: 252 ...
Weight loss coaching
Coaches use many techniques. Borrowing the concept of modeling from NLP, a weight loss coach will identify the behaviors ... One of the first texts to highlight the relationship between behavior, culture and weight gain. In her book Susie looks at how ...
Luzhou Laojiao
"Luzhou Spirit Brewing Techniques". China Intangible Cultural Heritage. Ministry of Culture, P.R.China. Archived from the ...
Paracas culture
Yarns were also dyed in a wide range of hues, used together in loom weaving and many other techniques. This combination of ... While the Paracas culture developed in this region between approximately 1200 BCE and 100 BCE, the Topará culture is thought to ... Nazca Culture and iconography are believed by scholars such as Helaine Silverman to have evolved from Paracas culture. Hendrik ... The two cultures coexisted for one or more generations, both on the Paracas Peninsula and in the nearby Ica Valley. Their ...
Body culture studies
Frankfurt/M.: Suhrkamp, vols.1-2. Mauss, Marcel 1934: "Les techniques du corps." - English 1973: "Techniques of the Body." In: ... "Culture" in singular is an abstraction. The study of body culture is always a study of body cultures in plural. Body cultures ... The study of different class habitus (→class culture), youth cultures, gender cultures (→gender identity) etc. opened up for ... As body culture studies analyse culture and society in terms of human bodily practices, they are sometimes viewed as a form of ...
Microfluidics in chemical biology
Cell culture techniques, Microfluidics). ... This technique is relatively cheap and can be used to make ... Young EW, Beebe DJ (March 2010). "Fundamentals of microfluidic cell culture in controlled microenvironments". Chem Soc Rev. 39 ... culture cells as well as perform many other important biological studies. One unique feature that results from miniaturization ... can either lend to the advantages of using microfluidics or it can necessitate further refinement of experimental technique. In ...
National Highways & Motorway Police
Training strategy After study of the current policing system and the culture... a comprehensive training strategy is devised to ... Main elements of the training strategy in NHMP are: • TNA to assess training needs • SWOT Analysis Training Techniques • ... Quality Instructors • Modern Techniques of Instruction 1.Syndicate/Participative System 2.Simulation Exercises 3.Case Studies 4 ...
Yemenite silversmithing
... after its technique. Until then, most were prepared by using casting techniques, and embossing and fashioning from solid pieces ... Yemeni culture, Jewish culture, Jews and Judaism in Yemen, Jewish Yemeni history, History of Yemen). ... Today, in modern techniques, they use either a thin metal wire which is melted or plate of same substance. The purpose of the ... Granulation was a common form of decorative technique used in the making of rings, bracelets and large necklaces. Granulated ...
Vichy shower
Miller, Erica T. (1996). SalonOvations' Day Spa Techniques. Cengage Learning. ISBN 1562532618. Whitney, Nicole. "What Are the ... v t e (Bathing, All stub articles, Culture stubs). ...
Freewill (song)
Popular Culture and Philosophy. Vol. 57. Open Court Publishing Company. ISBN 9780812697162. Popoff, Martin (2017). Rush: Album ... Technique. Hal Leonard Corporation. 2015. ISBN 9781495032714. Berti, Jim; Bowman, Durrell (2011). Reisch, George A. (ed.). Rush ...
Chen Houei-kuen
10.2003 on 8 October, National Culture and Arts Foundation 7th National Literary Award. Taiwanese art 1. 家庭美術館 美術家傳記: 陳慧坤 2.蒼松映 ... In order to study Eastern and Western art ideology and techniques, Chen made two research trips to Japan in the 1950s which ... In addition, he also received the Culture Award of Executive Yuan (1997), The Art & Business Awards of the Council for Cultural ... 5.1997 on 29 December, issued by the Executive Yuan, Executive Yuan Culture Award. 6.1999 on 26 April, issued by the Council ...
Union Village Shaker settlement
In economic affairs, they increasingly adapted the methods of the world: taking out loans, using mass marketing techniques. ... dual aspects of Shaker impacts at the close of their way of life at Union Village as both warming to mainstream worldly culture ...
Chilean art
Manuel Ramirez Rosales who inherited much of his technique and his colour schemes as well as a fascination with French culture ... His technique largely resembles the Cuzco School so his portrayal of faces was not particularly accurate, appearing flat and ... The attempt to bring the European model to Chile by replicating its techniques has been seen as a failure in terms of the ... The use of art in this time could be aesthetic, practical, ritual or religious, depending on the culture and the resources ...
Dialogue
Educators such as Freire and Ramón Flecha have also developed a body of theory and techniques for using egalitarian dialogue as ... Una difesa filosofica del confronto pacifico tra culture", Carocci, Roma, 2011 Suitner, Riccarda (2022) The Dialogues of the ... From Dialogical Leadership to a Dialogical Corporate Culture. Heidelberg: Menon 2013. p. 10. Dietz: Acting Independently for ... apart from rational deliberations or culture wars); dramatization to call widespread attention to the issue at hand; and, ...
Protocol Wars
Cerf said the social culture (group dynamics) that first evolved during the work on the ARPANET was as important as the ... Abbate 2000, p. 38 The NPL group influenced a number of American computer scientists in favor of the new technique, and they ... Simply, they possess the Internet culture, are interested in it and, notably, participate in IETF." Russell notes that Cohen, ... They believed speech traffic would continue to dominate data traffic and believed in traditional telegraphic techniques. Baran ...
Christopher Wylie
On March 27, 2018, Wylie provided evidence to the Digital, Culture, Media and Sport Committee of the UK Parliament that ... In November 2015, Eunoia Technologies pitched Facebook data-mining techniques to the Liberal Party of Canada, securing a $ ... then you have won that culture war." Wylie has said he did not realize the "potential misuse" of his research at the time, ... Written evidence submitted by Aleksandr Kogan to the Digital Culture, Media and Sport Select Committee', House of Commons (UK ...
Yessentuki
... applying special techniques of therapeutic physical training. Within the framework of administrative divisions, it is ... health resort in the last third of the 19th and the early 20th centuries attracted famous representatives of Russian culture, ...
Assyria
The culture of the Assyrian elite was strongly influenced by Babylonia in the south; in a vein similar to how Greek ... and the development of new and innovative siege warfare techniques. At the height of the Neo-Assyrian Empire, the Assyrian army ... Ethnicity and culture are largely based in self-perception and self-designation. A distinct Assyrian identity seems to have ... Though in some aspects influenced by Assyrian culture, these states were for the most part not ruled by Assyrian rulers. Assur ...
Die Gstettensaga: The Rise of Echsenfriedl
The absurdist comedy deals with the politics and hype behind media technology and nerd culture. Grenzfurthner calls his film a ... In their infamous 1972 book Anti-Oedipus, Gilles Deleuze and Félix Guattari have identified the technique of bricolage as the ... France Culture / Place de la toile politique de l'algorithme; May, 2014 IFJ / Phuture Fest Echsenfriedl fan art, monochrom/blog ... Net culture magazine Furtherfield calls it a "hackploitation art house film" that reimagines "the makerspace as grindhouse", a ...
Digital self-determination
"Explanatory momerandum to the Age Appropriate Design Code". The U.K. Department for Digital, Culture, Media & Sport. 11 June ... the adoption of obfuscation techniques as a way of evading and protesting digital surveillance). Internet activist Eli Pariser ... The neo-colonial structures and discourses are already part of the different "postcolonial" cultures. The postcolonial era in ... Indian Country: Essays on Contemporary Native Culture. Waterloo: Wilfrid Laurier University Press. Alfred, T. 2009. Wasáse: ...
Execution by elephant
"The Empire of the Great Mughals: History, Art and Culture". Reaktion Books, February 2004. ISBN 1-86189-185-7 Tennent, Emerson ... "used this technique to chastise 'rebels' and then in the end the prisoners, presumably much chastened, were given their lives ... Annemarie Schimmel (2004). The Empire of the Great Mughals: History, Art and Culture. Reaktion Books. p. 96. ISBN 9781861891853 ... Elephants in Indian culture, Execution methods, Social history of India, Social history of Africa, History of South Asia, ...
Photovoice
"Comicvoice: Community education through sequential art." Pop Culture Association - American Culture Association, St. Louis, MO ... or picturevoice which includes techniques such as photo-elicitation and digital storytelling. These techniques allow research ...
Human geography
It draws heavily on Marxist theory and techniques, and is associated with geographers such as David Harvey and Richard Peet. ... cultures, economies, and their interactions with the environment. It analyzes spatial interdependencies between social ... links to possibilism and cultural ecology some of the same notions of causal effect of the environment on society and culture ... geografie Transactions of the Institute of British Geographers Geography portal AP Human Geography Concepts and Techniques in ...
Benjamin Franklin
In the same year, he printed a new currency for New Jersey based on innovative anti-counterfeiting techniques he had devised. ... Olsen, Neil C. (2013). Pursuing Happiness: The Organizational Culture of the Continental Congress. Nonagram Publications. ISBN ... a common decision-making technique, now sometimes called a decisional balance sheet: ... my Way is, to divide half a Sheet of ... Philosophers of culture, Philosophers of education, Philosophers of ethics and morality, Philosophers of history, Philosophers ...
Health realization
Blaha, who resigned as chairman of Orthopedics at WVU, was quoted as criticizing the institute as being part of a culture at ... There are no steps, no uniformly appropriate internal attitudes, and no techniques within it. The "health of the helper" is ... Health Realization ("HR"), like Sydney Banks's philosophy, is deliberately not taught as a set of "techniques" but as an ... and culture. This level of organizational influence is impressive when the relatively modest level of VVCRP staff time and ...
Cauliflower ear
Martial arts culture, Wrestling culture, Rugby league terminology, Rugby union terminology). ... There are many described techniques for the drainage of blood in the acute stage to prevent hematoma, including simple needle ... This has been achieved with many techniques including: direct pressure dressings, in and out mattress sutures, buttons placed ...
Carlos the Jackal
... a lady's man whose favorite pick-up technique is tricking women by vaguely implying he is an international terrorist named " ... "Carlos director Olivier Assayas on the terrorist who became a pop culture icon". The Guardian. London. Archived from the ...
PLATO (computer system)
The techniques of responsive evaluation and portrayal were used combined with Context, Input, Process, Product (CIPP) and ... Bitzer was more forthright about CDC's failure, blaming their corporate culture for the problems. He noted that development of ...
DJ Kool Herc
... a technique by which the deejay switched from break to break at the height of the party. This technique is specifically called ... Since this emerging culture was still without a name, participants often identified as "b-boys", a usage that included and went ... Grandmaster Flash suggests that Herc may not have kept pace with developments in techniques of cueing (lining up a record to ... him with an education in New York City street culture. He began running with a graffiti crew called the Ex-Vandals, taking the ...
Deutsches Rechtswörterbuch
In order to capture the full lexical diversity of meanings for each word, the dictionary employs techniques of synchronic and ... As Germany's Federal President Richard von Weizsäcker once wrote: "The Rechtswörterbuch incorporates language- and culture- ...
Multimethodology
Rorty introduces his own ideas into pragmatism which includes the importance of culture, beliefs, and context. He shifts from ... Quantitative research requires skills of data-analysis and several techniques of statistic reasoning, while qualitative ...
Kakekotoba
It is a technique devised to enrich the way of conveying a poem in a limited space. The general pattern is as follows: Using ... Figurative Elements in Koto and Bunraku Music and Their Analogues in Related Forms of Japanese Culture. Osaka University, Japan ... The provenance of the technique is unclear, however it is likely it was already in common use in the period before writing was ... In English a similar technique is sometimes employed in both poetic and prose language. One contemporary example is: "They say ...
Protestant culture
... it presents a theory that science changes due to an accumulation of observations and improvement in experimental techniques and ... Christian culture Role of the Christian Church in civilization The Reformation and its influence on church architecture ... W. Williams, Peter (2016). Religion, Art, and Money: Episcopalians and American Culture from the Civil War to the Great ...
Need to Feel Your Love
Rindner, Grant (July 18, 2017). "Sheer Mag: Need to Feel Your Love". Spectrum Culture. Retrieved January 23, 2018. Levinson, ... one reviewer comparing this technique favorably to the works of The Hold Steady. However, Levinson was more mixed on the album ...
Differential interference contrast microscopy
The technique was developed by Polish physicist Georges Nomarski in 1952. DIC works by separating a polarized light source into ... DIC is used for imaging live and unstained biological samples, such as a smear from a tissue culture or individual water borne ... 1. Unpolarised light enters the microscope and is polarised at 45°. Polarised light is required for the technique to work. 2. ... Its resolution[specify] and clarity in conditions such as this are unrivaled among standard optical microscopy techniques. One ...
Nonviolent video game
Criticism for the violent aspects of video game culture has come from a number of anti-violence groups, and perhaps the most ... Advertisement placement and other marketing techniques have in the past targeted women as more receptive to non-violent video ...
Scarring hair loss
New diagnostic techniques, such as trichoscopy may be used for non-invasive differential diagnosis of cicatricial alopecia. ... In addition, if pustules are present, cultures are taken to identify which microbes, if any, may be contributing to the ...
Applause
The same technique is used in German-speaking countries to express approval at meetings. In Jamaica people may bang lids and ... In Deaf culture, Deaf audiences will use a more visually expressive variant of clapping. Instead of clapping their palms ... The age of the custom of applauding is uncertain, but it is widespread among human cultures. The variety of its forms is ... However, in a situation more specific to hearing culture, the traditional clap is used. In German-speaking countries, it is ...
Fieldnotes
The technique has four main parts: A field-worthy notebook where one records direct observations as they are being observed. A ... Additionally, in social science research that involves studying culture, it is important to faithfully capture language and ... The Grinnell technique has been regarded by many ornithologists as one of the best standardized methods for taking accurate ... Develop one's own transcribing language: While no one technique of transcribing (or "jotting") is perfect, most qualitative ...
Luitré
Base Mérimée: Search for heritage in the commune, Ministère français de la Culture. (in French) v t e (Articles with French- ... Amenities: Pharmacy, Doctor, Mairie, small Post Office, carwash, Automat laundry, Technique Controle - repair garage, ...
Inscription : Biomimétisme(s), Rencontres Techniques&Culture n°4
Appendix: Water Sampling Strategies and Culture Techniques for Detecting Legionellae
Browsing Regional Publications by Subject "Cell Culture Techniques"
Neonatal Conjunctivitis (Ophthalmia Neonatorum) Workup: Approach Considerations, Culture and Histology, Newer Diagnostic...
Newer Diagnostic Techniques. Nucleic acid amplification tests such as polymerase chain reaction (PCR) and transcription- ... Culture and Histology. Bacterial cultures on blood and chocolate agar are indicated in every case of neonatal conjunctivitis ... Culture of corneal epithelial cells for HSV if cornea is involved; PCR should also be considered in cases of possible HSV ... HSV infections may be more rapidly diagnosed with PCR, and PCR testing for HSV is more sensitive than viral culture. [11] ...
Browsing by Subject "Cell Culture Techniques"
Workshop on Cell Culture Techniques and Internal Quality Control, Hong Kong, 1-6 December 2003 : report ... Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella ... A simple aseptic method for the rapid removal of samples from small-scale cultures of micro-organisms / by S. G. Williams, W. H ... Use of cell culture in virology for developing countries in the South-East Asia Region ...
Collaborative Reduces CLABSI Rates Through Techniques, Teamwork and Culture Change
... .social-ris-container { display: flex; ... Culture Change is the Key to Success. The SLUG Bug collaborative was one of the first to utilize orchestrated testing to ... But it took more than procedures and techniques to reduce CLASBI rates. It took teamwork, collaboration and what I believe is ... But what we quickly found out was that other methods of central line care, include tubing change technique, hub care monitoring ...
Richard Feynman's 'Notebook Technique' Will Help You Learn Any Subject--at School, at Work, or in Life | Open Culture
Support Open Culture. Were hoping to rely on our loyal readers rather than erratic ads. To support Open Cultures educational ... Open Culture was founded by Dan Colman. We use cookies on our website to give you the most relevant experience by remembering ... Based in Seoul, Colin Marshall writes and broadcasts on cities and culture. Hes at work on a book about Los Angeles, A Los ... Richard Feynmans "Notebook Technique" Will Help You Learn Any Subject-at School, at Work, or in Life. in Education, ...
Sandor Katz's Fermentation Journeys: Recipes, Techniques, and Traditio - Cultures For Health
We want you to be nothing but successful here at Cultures for Health. Have Questions? Check out these resources to get you the ... Wherever hes gone, he has gleaned valuable insights into the cultures and traditions of local and indigenous peoples, whether ... Sandor Katzs Fermentation Journeys: Recipes, Techniques, and Traditions from around the World. ... Sandor Katzs Fermentation Journeys: Recipes, Techniques, and Traditions from around the World ...
Traditional knowledge and techniques associated with Pasto Varnish mopa-mopa of Putumayo and Nariño - intangible heritage -...
Pablo Trejo/Ministry of Culture of Columbia, 2019 The traditional knowledge and techniques associated with Pasto Varnish mopa- ... Carlos René Quintero/Ministry of Culture of Colombia, 2019. © Carlos René Quintero/Ministry of Culture of Colombia, 2019. © ... Carlos René Quintero/Ministry of Culture of Colombia, 2019. © Carlos René Quintero/Ministry of Culture of Colombia, 2019. © ... Carlos René Quintero/Ministry of Culture of Colombia, 2019. © Carlos René Quintero/Ministry of Culture of Colombia, 2019. © ...
Phrom Lok: Where traditional southern Thai culture meets modern-day farming techniques - Ambient Clock
Understanding Culture Appropriation: Explanations and Techniques to Avoid It - Grady Newsource
Understanding Culture Appropriation: Explanations and Techniques to Avoid It. *. * by Grady Capstone Journalist December 28, ... Why Its Newsworthy: Its an issue that can affect minority cultures whose culture they believe is being misappropriated. ... As holidays pass year after year, a practice now known as culture appropriation happens as well. ...
New techniques for biopsy and culture of human olfactory epithelial neurons
A new method of culturing the olfactory epithelium was devised. This slice culture technique improved the success rate for ... Four culture techniques were performed, including whole explants and explanted .... View more ,Objective To improve the success ... Four culture techniques were performed, including whole explants and explanted biopsy slices. Five days after plating, neuronal ... The techniques presented here make the human olfactory epithelium a useful model for clinical research into certain olfactory ...
CSP - Glossaire MDN : définitions des termes du Web | MDN
Technique 22: Presentation of Self, Material Culture, Poverty
| TRAILS: Teaching Resources and...
"Technique 22: Presentation of Self, Material Culture, Poverty". TRAILS: Teaching Resources and Innovations Library for ... Erin K Anderson, A Quest for Culture in the Campus Community , TRAILS: Teaching Resources and Innovations Library for Sociology ... Keith D. Wilkins, Culture Through Everyday Objects , TRAILS: Teaching Resources and Innovations Library for Sociology: All ... Paul D. Starr, Technique 11: Historical Time: Modern Industrial Society in PerspectiveTeaching Objectives/Student Learning ...
Tissue Culture Techniques | Profiles RNS
"Tissue Culture Techniques" by people in this website by year, and whether "Tissue Culture Techniques" was a major or minor ... A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured ... "Tissue Culture Techniques" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Tissue Culture Techniques" by people in Profiles. ...
Fluroescent antibody technique for identification of presumptively positive gonococcal cultures.
Title : Fluroescent antibody technique for identification of presumptively positive gonococcal cultures. Personal Author(s) : ... Bacteriological Techniques Fluorescent Antibody Technique Indicators And Reagents Methods Neisseria Gonorrhoeae Research ... Direct FA technique using Flazo orange counterstain in identification of Neisseria gonorrhoeae Cite ... Title : Direct FA technique using Flazo orange counterstain in identification of Neisseria gonorrhoeae Personal Author(s) : ...
Basel Media Culture and Cultural Techniques
... Das Seminar für Medienwissenschaft der Universität Basel veröffentlicht seit 2020 ... Die Basel Media Culture and Cultural Techniques (BMMCT) Working Papers erscheinen in unregelmässigen Abständen in deutscher und ... Potent Mercantile Metaphors of the Programming Language JAVA (Basel Media Culture and Cultural Techniques Working Papers 3). ... Seeing Aesthetically and Scientifically (Basel Media Culture and Cultural Techniques Working Papers 5). Hannes Bajohr (2020): ...
Methodology and Techniques of Archaeological Research (S) (2004/2005)-Dep.Cultures and Civilizations-University of Verona
A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation<...
A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. In: Cell ... A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. Cell ... A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. / Dorko ... A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. ...
A Witch's World of Magick: Expanding Your Practice with Techniques & Traditions from Diverse Cultures - Hex: Old World Witchery
Inspiration from the Worlds Great Traditions Join Melanie Marquis as she explores an amazing assortment of magickal techniques ... A Witchs World of Magick: Expanding Your Practice with Techniques & Traditions from Diverse Cultures - by Melanie Marquis ... Home / A Witchs World of Magick: Expanding Your Practice with Techniques & Traditions from Diverse Cultures - by Melanie ... and an abundance of other techniques. With these new perspectives on the common threads that weave throughout our magickal ...
Action of drug compounds in tissue revealed by new technique - University of Birmingham
A new technique that can analyse how drug molecules bind to proteins in tissue samples could offer an improved route to drug ... Cell cultures enable the effects of different compounds to be tested on specific biological targets involved in various ... Action of drug compounds in tissue revealed by new technique. A new technique that can analyse how drug molecules bind to ... This new technique, described in a paper published today (14 July 2022) in Angewandte Chemie, enables researchers to use real ...
Supersize Me Essay - 473 Words | Internet Public Library
Rhetorical Techniques Used In Supersize Me. 705 Words , 3 Pages. *. Eric Schlosser And Charles Wilsons Chew On This. 594 Words ... Schlossers Fast Food Culture. 328 Words , 2 Pages. Fast food nation spilled many incriminating facts about Americas fast food ... Rhetorical Techniques Used In Supersize Me. 705 Words , 3 Pages. In 2003, a brave, middle-aged man named Morgan Spurlock ... Eminent voices in the food culture convince people that McDonalds is unhealthy. Most processed foods are considered unhealthy ...
Double Your Revenue Using This Simple, Time-Tested Technique
Structural Biochemistry/Protein sequence determination techniques/Mass Spectroscopy - Wikibooks, open books for an open world
The cell culture is grown in a "light" medium and a "heavy" medium. These mediums are given identical conditions except for one ... 7.1 Labelling Techniques *7.1.1 Isotope-Coded Affinity Tag (ICAT). *7.1.2 Stable Isotope Labeling with Amino Acids in Cell ... This technique is often coupled with mass spectrometry for protein analysis. History of ESI[edit , edit source]. The research ... Labelling Techniques[edit , edit source]. Before peptide tagging was used to study protein dynamics, quantitative proteomics ...
DVD Review: Guitar DVD - Total Scales Techniques And Applications By Mark John Sternal | Blogcritics
Home/Culture and Society/Science and Technology/DVD Review: Guitar DVD - Total Scales Techniques And Applications By Mark John ... Lesson 9, More Techniques here continues additional techniques such as pull offs, hammer pull/pull hammer, hammer pull with ... Lesson 12, Tremolo/Whammy Bar Techniques describes the technique of using the whammy bar that is available on certain ... DVD Review: Guitar DVD - Total Scales Techniques And Applications By Mark John Sternal. T. Michael Testi January 9, 2008 1 ...
Call for paper: "What Gender Makes" - Le carnet de Techniques & Culture
Technique & culture. The journal Techniques & culture is devoted to the pragmatic, social and symbolic dimensions of techniques ... Le carnet de Techniques & Culture est le blog de la revue danthropologie des techniques. Vous pourrez suivre les débats et ... Conference: "Les Rencontres Techniques&Culture", Marseille, January 2021. An androcentric history of technologies. The history ... This issue of Techniques&Culture aims to elaborate on this program-which still remains to be fully explored-through case ...
PAI-2 human recombinant, expressed in E. coli, 95 (SDS-PAGE), 95 (HPLC), cell culture tested Plasminogen Activator Inhibitor-2
Chapitre 10. Les prêts immobiliers - Pratique des techniques bancaires [Book]
La loi … - Selection from Pratique des techniques bancaires [Book] ... Culture générale. Une première version de la loi Scrivener est entrée en vigueur le 10 janvier 1978 ; elle a été ensuite ... Pratique des techniques bancaires by Aurélien Giraud. Get full access to Pratique des techniques bancaires and 60K+ other ... Get Pratique des techniques bancaires now with the OReilly learning platform.. OReilly members experience live online ...
Transcendental Meditation technique - Wikipedia
... whose goal is to create a better culture."[22] Charles H. Lippy writes that earlier spiritual interest in the technique faded ... The Transcendental Meditation technique (abbreviated as TM) is the technique associated with the practice of Transcendental ... Transcendental Meditation (TM), a concentrative technique ... has been the most extensively studied meditation technique.. ... For example, TM is simply - as they state - a technique. Although it enables one to cope with life, it offers no goal beyond ...
Cells2022MeSHExplantsSpecimensSensitivityAsepticPERFUSIONDiagnosisPracticeSputumOrganismsTissue Culture TechniquesDiagnostic TechniquesLaboratoryAnalyticalMethodsEmbryo2021Describes the techniqueGrownCRISPRAntigenMicroscopyNative AmericanCultivationClinicalNeuronsSterileAntibodyChlamydiaTraditions1978SearchDescribeSpeciesProductiveExploreEmploysEvaluationExplores2000SerumBiologicalToxicityPreservationAdultLearnMediumInfection
Cells15
- Since Chlamydia bacteria are obligate intracellular organisms, the culture specimens need to contain epithelial cells and not just exudative material. (medscape.com)
- Anderson DG, Markova D, An HS, Chee A, Enomoto-Iwamoto M, Markov V, Saitta B, Shi P, Gupta C, Zhang Y. Human umbilical cord blood-derived mesenchymal stem cells in the cultured rabbit intervertebral disc: a novel cell source for disc repair. (rush.edu)
- Part of the early stages of drug discovery takes place in cell cultures, clusters of cells that are grown in the laboratory, outside of their natural environment. (birmingham.ac.uk)
- Requirements: Students should have completed at least one biology course and have an interest in learning common techniques for culture of animal cells. (missouriwestern.edu)
- The overall aims of this project are to study the effects of various probiotic bacteria or prebiotics on intestinal inflammation, using cultured mammalian cells and/or mice as experimental models. (missouriwestern.edu)
- Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency. (labograde.com)
- An in vitro BBB model with a co-culture of rat brain microvascular endothelial cells (RBECs) and microglia cells using Transwell chambers was established to assess the RSV delivery across BBB. (nih.gov)
- Cell culture is the process of taking cells out of the body and growing them in an artificial environment. (grstiftung.ch)
- To date, standard cell culture comprises that cells live in a soup of nutrients on the surface of relatively simple plastic dishes. (grstiftung.ch)
- We have designed novel biomimetic cell culture surfaces that imitate the softness of various tissues and organs, allowing the cells to «feel like home» even outside the body. (grstiftung.ch)
- A second aspect of the project consists in quantifying the benefits of culturing cells on our innovative cell culture system. (grstiftung.ch)
- The coating does not interfere with conventional biological techniques to retrieve cells for biochemical or molecular analysis. (grstiftung.ch)
- Further experiments have been carried out to characterize the behavior of cells grown on our innovative culture substrates. (grstiftung.ch)
- Cytology is used mainly for diagnosing infant inclusion conjunctivitis and ocular trachoma through the demonstration of intracytoplasmic C trachomatis inclusions in HeLa cells (ie, continuously cultured carcinoma cell line used for tissue cultures). (medscape.com)
- C trachomatis grows well in a variety of cell lines (eg, McCoy and HeLa cells) that can be maintained in tissue culture. (medscape.com)
20221
- This new technique, described in a paper published today (14 July 2022) in Angewandte Chemie, enables researchers to use real tissue samples to assess which proteins the drug will bind to in the body and therefore how effective it is likely to be against the target. (birmingham.ac.uk)
MeSH1
- Tissue Culture Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
Explants1
- Nodal explants were cultured on MS medium containing various concentrations of KIN (0, 0.5, 1 and 1.5mg.l-1) in combination with IBA (0, 0.025 and 0.5mg.l-1). (who.int)
Specimens5
- This technique allowed for the isolation of human hepatocytes on a large scale (up to 18.5 × 10 9 in one case) from normal and diseased liver specimens. (utmb.edu)
- We conclude that the technique presented here allows isolation of large numbers of human hepatocytes from specimens unsuitable for transplantation but very valuable for biomedical research. (utmb.edu)
- Conclusive diagnosis of these viruses requires electron microscopic examination of stool specimens, a laboratory technique that is available only at a few large centers, including CDC. (cdc.gov)
- Suspected and confirmed SARS-CoV-2 positive clinical specimens, cultures, or isolates should be packed and shipped as UN 3373 Biological Substance, Category B. (cdc.gov)
- Cell culture is also preferred for rectal specimens because nonculture test results are difficult to interpret in the presence of stool organisms. (medscape.com)
Sensitivity3
- Next steps for the research will include improving the sensitivity of the technique, and extending it to other types of drug compounds. (birmingham.ac.uk)
- Comparison of both culture -independent techniques can provide a general overview of the whole process, although DGGE does not reveal the diversity expected due to the reported problems with the sensitivity of this technique . (bvsalud.org)
- Because of its high specificity (100%) and sensitivity, cell culture is the only test that should be used to establish the presence or absence of infections in cases with legal implications, such as those involving rape or sexual abuse. (medscape.com)
Aseptic2
- Students will learn basic tissue culture techniques, including aseptic techniques, maintenance and propagation of cultured cell lines, and assays for analysis of cellular functions. (missouriwestern.edu)
- Experience in aseptic technique, cell culture, and retroviral or lentiviral transductions. (nih.gov)
PERFUSION2
- A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. (rush.edu)
- Large numbers of human hepatocytes were obtained from split and whole livers by using an adaptive form of the collagenase perfusion technique employed in rodent and human biopsies. (utmb.edu)
Diagnosis3
- The presence of HSV in tissue culture remains the criterion standard in the diagnosis of HSV, despite a high false-negative rate. (medscape.com)
- To compare the performance of the Ogawa-Kudoh method with the modified Petroff technique in diagnosis of pulmonary tuberculosis. (scielo.br)
- The Ogawa-Kudoh technique proved to be sufficiently sensitive and specific for diagnosis of pulmonary tuberculosis, and, therefore, suitable for routine laboratory application. (scielo.br)
Practice6
- But what we quickly found out was that other methods of central line care, include tubing change technique, hub care monitoring, CVC access limitation and CVC removal monitoring, varied widely in local hospital practice. (infectioncontroltoday.com)
- Feynman had to go face-to-face with the elders of the Princeton physics department, but if you don't benefit from that kind of institutional threat, you might consider putting into practice another Feynman technique: "teaching" what you've learned to someone else. (openculture.com)
- As holidays pass year after year, a practice now known as culture appropriation happens as well. (uga.edu)
- Providing instructions on how to unite classic beliefs with modern practice, A Witch's World of Magick uncovers the universal principles that underlie decoy magick, curse breaking, potion making, number magick, and an abundance of other techniques. (hexwitch.com)
- The Transcendental Meditation technique (abbreviated as TM ) is the technique associated with the practice of Transcendental Meditation developed by the Indian spiritual figure Maharishi Mahesh Yogi . (wikipedia.org)
- The author praises the congress initiative of highlighting the metapsychological writings as a place for dialogues with practice, technique and culture. (bvsalud.org)
Sputum1
- A bronchoscopic culture is done to find infection in the lung that cannot be accurately detected by a sputum culture . (medlineplus.gov)
Organisms3
- No organisms are seen on the culture. (medlineplus.gov)
- Not all organisms found with bronchoscopic culture need to be treated. (medlineplus.gov)
- Approximately 30% of the organisms contained in the microbiome have been cultured, but not all organisms can be grown in vitro with current techniques. (medscape.com)
Tissue Culture Techniques2
- This graph shows the total number of publications written about "Tissue Culture Techniques" by people in this website by year, and whether "Tissue Culture Techniques" was a major or minor topic of these publications. (rush.edu)
- Below are the most recent publications written about "Tissue Culture Techniques" by people in Profiles. (rush.edu)
Diagnostic Techniques1
- Until the 1970s, diagnostic techniques for infectious diarrhea were limited to bacteria and protozoa, and an etiologic agent could be identified in a limited proportion of cases. (cdc.gov)
Laboratory5
- Bronchoscopic culture is a laboratory exam to check a piece of tissue or fluid from the lungs for infection-causing germs. (medlineplus.gov)
- Any laboratory that cultures Legionella from environmental samples is encouraged to enroll. (cdc.gov)
- Does the laboratory routinely perform culture for Legionella ? (cdc.gov)
- If you need samples tested during an investigation into cases of disease, you should consider using a laboratory that has documentation from a proficiency testing program like the Environmental Legionella Isolation Techniques Evaluation Program. (cdc.gov)
- Cultures of SARS-CoV-2 should be handled in a Biosafety Level 3 (BSL-3) laboratory using BSL-3 practices, and inoculation of animals with infectious wild-type SARS-CoV-2 should be conducted in an Animal Biosafety Level 3 (ABSL-3) facility using ABSL-3 practices and respiratory protection. (cdc.gov)
Analytical1
- Mass spectrometry is an analytical technique used for identifying the mass of a compound based on the mass-to-charge ratio of charged particles. (wikibooks.org)
Methods6
- Bacterial cultures on blood and chocolate agar are indicated in every case of neonatal conjunctivitis and remain the criterion standard despite newer diagnostic methods. (medscape.com)
- Here he also shows methods of holding a pick while tapping as well as how this technique can be just as effective on acoustic as it is on electric. (blogcritics.org)
- The Ogawa-Kudoh and modified Petroff methods were efficient in mycobacteria detection, with no significant differences in results (p=0.549) and contamination rate of the cultures (p=0.065). (scielo.br)
- Our results are in agreement with those obtained with culture independent methods , but qPCR showed higher precision and a different behavior was observed for each yeast species and at each temperature assayed. (bvsalud.org)
- Majors must complete three required courses (listed below), one Area Focus and Regional Perspectives on Culture course, one anthropological methods course, one anthropological theory course and 15 credits of electives based on student interest. (syr.edu)
- Students in the major select at least one methods course, such as Methods in Archaeology, Ethnographic Techniques or Life Histories/Narratives. (syr.edu)
Embryo1
- Studies on embryo toxic effects of thallium using the whole embryo culture technique. (cdc.gov)
20211
- financed by the EEA Grants 2014-2021, within the RO-CULTURE Program. (muzeul-etnografic.ro)
Describes the technique2
- Lesson 12, 'Tremolo/Whammy Bar Techniques' describes the technique of using the whammy bar that is available on certain electric guitar models. (blogcritics.org)
- One canvasser describes the technique in rural areas as a 'social call' as in many rural locations people tend to know their local TD. (rte.ie)
Grown1
- The organism can be grown in culture using a specific liquid medium developed by Kelly. (medscape.com)
CRISPR2
- What has changed in recent years, however, is the advent of the technique known as CRISPR. (iflscience.com)
- Not required, but would be of great benefit: knowledge of iPSC culture, CRISPR design, and/or experience with bioinformatic analysis of next-generation sequencing data. (vanderbilt.edu)
Antigen1
- In infants with suspected chlamydial conjunctivitis, perform an antigen/DNA detection test, chlamydial culture, or both, using scrapings from the palpebral conjunctiva. (medscape.com)
Microscopy1
- Each biopsy specimen was bisected, and 1 piece was processed for immunohistochemistry or electron microscopy while the other piece was dissected further for explant culture. (edu.au)
Native American2
- At the same time, through photography, painting, sculpture and mixed media they celebrate the ongoing presence of Native American art and culture and remind us that art is an essential form of activism. (portlandartmuseum.org)
- Supported in part by Native Arts & Cultures Foundation and the Museum's Native American Art Council. (portlandartmuseum.org)
Cultivation1
- Productive soil and outstanding growing environments, together with cultured farming techniques and unconventional agricultural tools, are responsible for the optimal environments in productive pastoral, forestry, and cultivation activities. (worldatlas.com)
Clinical3
- The techniques presented here make the human olfactory epithelium a useful model for clinical research into certain olfactory dysfunctions and a model for the causes of neurodevelopmental and neurodegenerative diseases. (edu.au)
- EAC organoid cultures provide a pre-clinical tool for studies of clonal evolution and precision therapeutics. (nature.com)
- In addition, they are unsuitable for large numbers of patients (eg, in the emergency department [ED]). Nevertheless, in certain clinical situations, cultures are mandatory. (medscape.com)
Neurons2
- Objective To improve the success of culturing olfactory neurons from human nasal mucosa by investigating the intranasal distribution of the olfactory epithelium and devising new techniques for growing human olfactory epithelium in vitro. (edu.au)
- Cajal improved the techniques of Golgi, which involved staining neurons for observation. (giantmicrobes.com)
Sterile4
- A design matrix of eight distinct factor/level combinations (test groups) were developed by using four factors: sterile versus clean tubing change technique, hub care compliance monitoring versus not monitoring, CVC line entry access limitation versus not monitoring and CVC line removal tracking versus no tracking policy. (infectioncontroltoday.com)
- In addition, four health care organizations elected to implement the sterile tubing change technique after reviewing the results of the testing, which resulted in a 64 percent decrease in CLABSI rates from the baseline. (infectioncontroltoday.com)
- One of the approaches to managing this important pest is use of a sterile insect technique (SIT). (usda.gov)
- Sterile Insect Technique programs have been developed for management of several tephritid fruit fly pests. (usda.gov)
Antibody2
Chlamydia1
- In infants with suspected chlamydial pneumonia, perform a nasopharyngeal swab for Chlamydia culture. (medscape.com)
Traditions2
- Wherever he's gone, he has gleaned valuable insights into the cultures and traditions of local and indigenous peoples, whether they make familiar ferments like sauerkraut or less common preparations like natto and koji. (culturesforhealth.com)
- Magickal Inspiration from the World's Great Traditions Join Melanie Marquis as she explores an amazing assortment of magickal techniques gathered from the annals of world folk magick. (hexwitch.com)
19781
- In 1978, the same technique was used to detect rotavirus, the most common cause of severe diarrhea in children. (cdc.gov)
Search1
- Does Language and Culture Affect Search Techniques? (searchenginejournal.com)
Describe2
- In this excerpt from the programme, canvassers describe their techniques of door-to-door canvassing. (rte.ie)
- Military officials refused to describe what techniques Peterson had objected to and said all records of them had been destroyed. (democracynow.org)
Species1
- Culture-dependent and independent techniques to monitor yeast species during cold soak carried out at different temperatures in winemaking. (bvsalud.org)
Productive1
- Scott teaches non-violent communication techniques that help managers live healthier productive lives. (udemy.com)
Explore4
- Lesson 18, 'Exotic Scales' explore the scales of other cultures from around the world. (blogcritics.org)
- Whether it's silence and solitude you're seeking or an itinerary of action-packed activities, we cover the best places to explore and appreciate Sámi communities and culture in Norway. (lonelyplanet.com)
- Ailey's style blended genre-including modern, ballet, jazz, and techniques of various African cultures-to explore the plight of the marginalized both onstage and off. (playbill.com)
- When I was opening Aska,' says chef Fredrik Berselius, 'the idea was to look at New York and its surroundings, then explore it through Scandinavian eyes and food… The food I serve aims to take the guest on a journey of Scandinavian flavours, memories, feelings, and techniques with an emphasis on ingredients from the Northeastern US. (quintessentially.com)
Employs2
- 2. The manufacturing employs traditional techniques or processes. (kyoto.jp)
- Gažiová employs the technique of graffiti and templates on natural canvas. (prague-art.cz)
Evaluation2
- The Environmental Legionella Isolation Techniques Evaluation Program issues documentation of proficiency to laboratories that have demonstrated the ability to isolate Legionella from simulated environmental samples by culture. (cdc.gov)
- What is the value to using a lab that has documentation of proficiency from the Environmental Legionella Isolation Techniques Evaluation Program? (cdc.gov)
Explores1
- Lesson 10, 'Tapping' explores the technique of hammering or tapping a fret and pulling off to an open or fretted note. (blogcritics.org)
20001
- That long ago one can't determine specific ethnic traits, but we know traits of Sámi culture appear from approximately 2000 years ago, and traces of burial sites and settlement continue right up to today's Sámi population. (lonelyplanet.com)
Serum1
- Most importantly, using testis explanted at 16.5 or 18.5 dpc and cultured for 2-4 days, we demonstrated that the kinetics of changes in H3K4me2, H3K4me3, global DNA methylation and on parental imprints can generally be reproduced ex vivo with the model of organ culture without the addition of serum. (nih.gov)
Biological1
- One recent improvement for mass rearing oriental fruit flies involves adding wheat germ oil (WGO) to the larval culture medium which improved several parameters of biological performance. (usda.gov)
Toxicity1
- Culture techniques: Applicability for studies on prenatal differentiation and toxicity: 5th symposium on prenatal development, May, 1981, Berlin. (cdc.gov)
Preservation1
- Preservation techniques and incorporating fermented ingredients have also long been a tradition in Scandinavian food culture. (quintessentially.com)
Adult2
- Conclusions This study explains and overcomes most of the variability in the success in observing neurogenesis in cultures of adult human olfactory epithelium. (edu.au)
- We considered the issue of whether WGO supplementation of the larval culture medium influences the fatty acid make up of adult tissues. (usda.gov)
Learn8
- Total Scales Techniques And Applications may be one of the fastest ways to learn how to play guitar. (blogcritics.org)
- Guitar DVD: Total Scales Techniques And Applications is a DVD training system that is geared for a beginner to intermediate guitar player who is just starting out to learn how to play guitar, as well as for the experienced guitarist who is self-taught and has had little, if any, formal instruction. (blogcritics.org)
- You will also learn picking techniques and how to work with a metronome. (blogcritics.org)
- Many of these techniques are really more geared for an electric guitar, but with some experience and adeptness, one can also learn to make this work for an acoustic guitar as well. (blogcritics.org)
- Here you will learn how to apply many of the techniques to the pentatonic scales. (blogcritics.org)
- Learn what features have made the most difference to the salon's culture, business, and successful operation. (nailsmag.com)
- There are myriad Sami-led tour companies in the region to learn more and experience some of their culture. (lonelyplanet.com)
- Students learn how to show respect in Filipino culture, they learn techniques using sticks and they wear handkerchiefs instead of belts to show their ranking. (wbaltv.com)
Medium1
- [ 52 ] In this technique, a couple of drops of blood are added to the medium, which is incubated at 30-37°C and kept for 2 to 6 weeks. (medscape.com)
Infection3
- Many hospitals across the country already have evidence-based practices in place to help improve central line infection rates, which include hand hygiene, central line insertion techniques, dressing changes and root cause analysis. (infectioncontroltoday.com)
- Abnormal culture results usually indicate a respiratory infection. (medlineplus.gov)
- infection diagnosed by culture. (cdc.gov)