Methods for maintaining or growing CELLS in vitro.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Techniques used in studying bacteria.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
Techniques used in microbiology.
A phenothiazine antipsychotic used principally in the treatment of NAUSEA; VOMITING; and VERTIGO. It is more likely than CHLORPROMAZINE to cause EXTRAPYRAMIDAL DISORDERS. (From Martindale, The Extra Pharmacopoeia, 30th ed, p612)
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A series of steps taken in order to conduct research.
Infections by bacteria, general or unspecified.
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Elements of limited time intervals, contributing to particular results or situations.
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Substances that reduce the growth or reproduction of BACTERIA.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Established cell cultures that have the potential to propagate indefinitely.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.

Studies on the response of ewes to live chlamydiae adapted to chicken embryos or tissue culture. (1/6081)

Ewes infected before gestation with chicken embryo or tissue culture adapted chlamydial strain B-577 were challenge inoculated with the homologous strain at four to 18 weeks of gestation. The ewes responsed with group specific complement fixing antibody titers of 1:8 to 1:256 by the second week after initial infection. A secondary antibody response in the surviving challenge inoculated ewes occurred at the time of lambing and reached titers of 1:32 to 1:256 by the second week after parturition. Group specific complement fixing antibodies did not appear to play a significant role in resistance to chlamydial infection. Ewes infected with the chicken embryo adapted strain B-577 excreted chlamydiae in their feces 60 days after inoculation. However, chlamydiae were not recovered from feces of ewes infected with the tissue culture adapted strain B-577. Placentas of ewes challenge inoculated by the intravenous route were consistently infected. Chlamydiae were recovered from placentas, some fetuses and lambs. In two instances when challenge inoculation was given by the intramuscular route, infection was detected only by the direct fluorescent antibody method.  (+info)

In vitro development of sheep preantral follicles. (2/6081)

Preantral ovarian follicles isolated from prepubertal sheep ovaries were individually cultured for 6 days in the presence of increasing doses of FSH (ranging from 0.01 to 1 microg/ml) and under two different oxygen concentrations, 20% and 5% O2. Follicle development was evaluated on the basis of antral cavity formation as well as the presence of healthy cumulus oocyte complexes. Follicle growth was enhanced by FSH addition to culture medium, while the use of a low oxygen concentration slightly stimulated this process. However, when follicles were cultured in the presence of high doses of FSH (1 microgram/ml) and under low oxygen concentration, a high proportion of them showed the presence of an antral cavity and of a healthy cumulus-oocyte complex. In addition, under this specific culture condition sheep preantral follicles released higher levels of estradiol as compared to those secreted at lower FSH concentrations or under 20% O2. When the meiotic competence of oocytes derived from follicles cultured at 1 microgram/ml FSH was assessed, no significant difference was recorded between the two oxygen groups. These results show that the culture conditions here identified are beneficial to in vitro growth and differentiation of sheep preantral follicles.  (+info)

Ontogeny of expression of a receptor for platelet-activating factor in mouse preimplantation embryos and the effects of fertilization and culture in vitro on its expression. (3/6081)

Platelet-activating factor (PAF; 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent ether phospholipid. It is one of the preimplantation embryo's autocrine growth/survival factors. It may act via a G protein-linked receptor on the embryo; however, the evidence for this is conflicting. The recent description of the intracellular form of the PAF:acetlyhydrolase enzyme as having structural homology with G proteins and Ras also suggests this as a potential intracellular receptor/transducer for PAF. This study used reverse transcription-polymerase chain reaction to examine the ontogeny of expression of the genes for these proteins in the oocyte and preimplantation-stage embryo. Transcripts for the G protein-linked PAF receptor were detected in the late 2-cell-stage embryo and in all stages from the 4-cell stage to blastocysts. They were also present in unfertilized oocytes and newly fertilized zygotes but only at relatively low levels. The incidence of expression was generally low and variable in late zygotes and early 2-cell embryos. Expression past the 2-cell stage was alpha-amanitin sensitive. The results indicated that mRNA for this receptor is a maternal transcript that was degraded during the zygote-2-cell stage. New expression of the receptor transcript required activation of the zygotic genome. Fertilization of embryos in vitro caused this transcript not to be expressed in the zygote. Culture of zygotes (irrespective of their method of fertilization) caused expression from the zygotic genome to be retarded by more than 24 h. This retardation did not occur if culture commenced at the 2-cell stage. The transcripts for the subunits of intracellular PAF:acetylhydrolase were not detected in oocytes or at any stage of embryo development examined, despite their being readily detected in control tissue. This study confirms the presence of the G protein-linked PAF receptor in the 2-cell embryo and describes for the first time its normal pattern of expression during early development. The adverse effects of in vitro fertilization (IVF) and embryo culture on the expression of this transcript may be a contributing factor for the poor viability of embryos produced in this manner. The reduced expression of PAF-receptor mRNA following IVF predicts that such embryos may have a deficiency in autocrine stimulation and also suggests that supplementation of growth media with exogenous PAF would be only partially beneficial. The effect of IVF and culture may also explain the conflicting literature.  (+info)

Steroid regulation of retinol-binding protein in the ovine oviduct. (4/6081)

Two studies were conducted to identify retinol-binding protein (RBP) expression in the ovine oviduct and to determine the role of ovarian steroids in its regulation. Ewes were salpingectomized on Days 1, 5, or 10 of their respective estrous cycles, and oviducts were homogenized for RNA analysis, fixed for immunocytochemistry (ICC), or cultured for 24 h for protein analysis. ICC localized RBP to the epithelium of all oviducts. RBP synthesis was demonstrated by immunoprecipitation of radiolabeled RBP from the medium of oviductal explant cultures. Explant culture medium from oviducts harvested on Day 1 contained significantly more RBP than medium from oviducts collected on Days 5 or 10. Slot-blot analysis demonstrated that steady-state RBP mRNA levels were significantly higher on Day 1 than Day 5 or 10. In the second experiment, ovariectomized ewes were treated with estradiol-17beta (E2), progesterone (P4), E2+P4 (E2+P4), or vehicle control, and oviducts were analyzed as above. P4 alone or in combination with E2 significantly reduced steady-state RBP mRNA levels compared to those in E2-treated animals. Oviductal explants from E2- and E2+P4-treated animals released 3- to 5-fold more RBP into the medium than control and P4 treatments as determined by ELISA. RBP synthesis of metabolically labeled RBP was increased by E2 and E2+P4 treatments. This study demonstrates that P4 applied on an estradiol background negatively regulates RBP gene expression in the oviduct whereas estradiol appears to stimulate RBP synthesis and secretion.  (+info)

Onset of nucleolar and extranucleolar transcription and expression of fibrillarin in macaque embryos developing in vitro. (5/6081)

Specific aims were to characterize the onset of nucleolar and extranucleolar transcription and expression of the nucleolar protein fibrillarin during preimplantation development in vitro in macaque embryos using autoradiographic and immunocytochemical techniques. Autoradiography was performed on whole embryos cultured with [3H]uridine for assessment of nucleolar (rRNA) and extranucleolar (mRNA) transcription. Expression of fibrillarin was immunocytochemically assessed in whole embryos using a primary antibody against fibrillarin and a fluorescein isothiocyanate-conjugated secondary antibody. Extranucleolar incorporation of [3H]uridine was first detected in 2-cell embryos cultured 6-10 h with [3H]uridine. Culture with alpha-amanitin prevented incorporation of label in 2-cell embryos, and treatment with ribonuclease reduced the signal to background levels, indicating that [3H]uridine was incorporated into mRNA and not rRNA or DNA. Nucleolar incorporation of [3H]uridine was not evident in pronucleate-stage or 2- to 5-cell embryos, but it was detected in one 6-cell embryo and in all 8-cell to blastocyst-stage embryos. Fibrillarin was first expressed in some 6- to 7-cell embryos, but it was consistently expressed in all 8-cell embryos. Fibrillarin was localized to the perimeter of the nucleolar precursor bodies, forming a ring that completely encapsulated these structures. Fibrillarin was not expressed in 8- to 16-cell embryos cultured with alpha-amanitin, indicating that it is transcribed, rather than recruited, at the 8-cell stage. In conclusion, in in vitro-fertilized macaque embryos developing in vitro, extranucleolar synthesis of mRNA is initiated at the 2-cell stage while the onset of nucleolar transcription occurs at the 6- to 8-cell stage, coincident with expression of fibrillarin.  (+info)

Investigation of distal aortic compliance and vasodilator responsiveness in heart failure due to proximal aortic stenosis in the guinea pig. (6/6081)

Hypotension and syncope are recognized features of chronic aortic stenosis. This study examined vasomotor responses and dynamic compliance in isolated abdominal aortae after chronic constriction of the ascending aorta. Guinea pigs underwent constriction of the ascending aorta or sham operation. Sections of descending aorta were removed for studies of contractile performance and compliance. Dynamic compliance was measured using a feedback-controlled pulsatile pressure system at frequencies of 0.5, 1.5 and 2.5 Hz and mean pressures from 40 to 100 mmHg. Chronic (149+/-6 days) aortic constriction resulted in significant increases in organ weight/body weight ratios for left ventricle (58%), right ventricle (100%) and lung (61%). The presence of heart failure was indicated by increased lung weights, left ventricular end-diastolic pressure and systemic vascular resistance, reduced cardiac output and increased levels of plasma atrial natriuretic peptide (166%), adrenaline (x20), noradrenaline (106%) and dopamine (x3). Aortic rings showed similar constrictor responses to phenylephrine and angiotensin II, but maximal vasodilator responses to acetylcholine and isoprenaline were significantly increased (144% and 48% respectively). Dilator responses to sodium nitroprusside, forskolin and cromokalim were unchanged. Compliance of all vessels decreased with increasing pulsatile frequency and to a lesser extent with increased mean pressure, but were similar in aortic-constricted and control groups. Chronic constriction of the ascending aorta resulted in heart failure and increased vasodilator responses to acetylcholine and isoprenaline in the distal aorta while dynamic compliance was unchanged. We hypothesize that increased endothelium-mediated vasodilatation may contribute to hypotension and syncope in patients with left ventricular outflow obstruction.  (+info)

Characterization of beta cells developed in vitro from rat embryonic pancreatic epithelium. (7/6081)

The present study evaluates the development and functional properties of beta cells differentiated in vitro. The authors have previously demonstrated that when E12.5 rat pancreatic rudiments are cultured in vitro in the absence of mesenchyme, the majority of the epithelial cells differentiate into endocrine beta cells. Thus, depletion of the mesenchyme provokes the expansion of endocrine tissue at the expense of exocrine tissue. The potential use of this procedure for the production of beta cells led the authors to characterize the beta cells differentiated in this model and to compare their properties with those of the endocrine cells of the embryonic and adult pancreas. This study shows that the beta cells that differentiate in vitro in the absence of mesenchyme express the homeodomain protein Nkx6.1, a transcription factor that is characteristic of adult mature beta cells. Further, electron microscopy analysis shows that these beta cells are highly granulated, and the ultrastructural analysis of the granules shows that they are characteristic of mature beta cells. The maturity of these granules was confirmed by a double-immunofluorescence study that demonstrated that Rab3A and SNAP-25, two proteins associated with the secretory pathway of insulin, are strongly expressed. Finally, the maturity of the differentiated beta cells in this model was confirmed when the cells responded to stimulation with 16 mM glucose by a 5-fold increase in insulin release. The authors conclude that the beta cells differentiated in vitro from rat embryonic pancreatic rudiments devoid of mesenchyme are mature beta cells.  (+info)

Long-term effects of prior heat shock on neuronal potassium currents recorded in a novel insect ganglion slice preparation. (8/6081)

Brief exposure to high temperatures (heat shock) induces long-lasting adaptive changes in the molecular biology of protein interactions and behavior of poikilotherms. However, little is known about heat shock effects on neuronal properties. To investigate how heat shock affects neuronal properties we developed an insect ganglion slice from locusts. The functional integrity of neuronal circuits in slices was demonstrated by recordings from rhythmically active respiratory neurons and by the ability to induce rhythmic population activity with octopamine. Under these "functional" in vitro conditions we recorded outward potassium currents from neurons of the ventral midline of the A1 metathoracic neuromere. In control neurons, voltage steps to 40 mV from a holding potential of -60 mV evoked in control neurons potassium currents with a peak current of 10.0 +/- 2.5 nA and a large steady state current of 8.5 +/- 2.6 nA, which was still activated from a holding potential of -40 mV. After heat shock most of the outward current inactivated rapidly (peak amplitude: 8.4 +/- 2.4 nA; steady state: 3.6 +/- 2.0 nA). This current was inactivated at a holding potential of -40 mV. The response to temperature changes was also significantly different. After changing the temperature from 38 to 42 degrees C the amplitude of the peak and steady-state current was significantly lower in neurons obtained from heat-shocked animals than those obtained from controls. Our study indicates that not only heat shock can alter neuronal properties, but also that it is possible to investigate ion currents in insect ganglion slices.  (+info)

In this study, expanded human trial with M, Gerbil kidney tissue culture inactivated HFRS vaccine was carried out and neutralizing antibody response was assessed by plaque reduction neutralization(PRNT) and CPE neutralization(CPENT) methods. According to the data of all 74 person immune sera assayed by the two methods, the rates of seroconversion and GMT tesed by CPENT were significantly higher than that by PRNT. Several vaccinating groups were studied and the neutralizing antibody levels were as follows: v...
View Notes - Problem_set_4 from BIS 104 at UC Davis. BIS104 Winter 2012 Problem Set 4 1. You briefly (about 15 minutes) label tissue culture cells with radioactive H 3 thymidine. You then look at
Alexis Carrels Tissue Culture TechniquesAlexis Carrel, the prominent French surgeon, biologist, and 1912 Nobel Prize laureate for Physiology or Medicine, was one of the pioneers in developing and modifying tissue culture techniques. The publicized work of Carrel and his associates at the Rockefeller Institute established the practice of long-term
Cell and Tissue Culture Supplies Market - Global Industry Analysis and Forecast to 2020. Global Cell and Tissue Culture Supplies Market is expected to reach USD 28,108.3 million in 2020.
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Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson. Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. npj Microgravity, 2017; 3 (1) DOI: 10.1038/s41526-017-0011-2 ...
Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson. Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. npj Microgravity, 2017; 3 (1) DOI: 10.1038/s41526-017-0011-2 ...
Question from Strategies for Enhancement in Food Production,aipmt,biology,class12,unit8,ch9,biology-and-human-welfare,strategies-for-enhancement-in-food-production
The laboratory employs human ocular cell and tissue culture models as experimental systems to understand the human condition and develop appropriate therapies. The laboratory is equipped with dedicated state-of-the-art analysis tools that facilitate the research programme. There is a good blend of experienced post-doctoral scientists and enthusiastic students eager to learn. The group has international standing within our area of expertise and laboratory members get the opportunity to present their work across the world at meetings in North America, Asia and Europe. This experience is valuable to the development of individuals and the team as a whole. ...
Final filtration of drugs and biological, final filtration of pure water for dialysis, diagnostics, final filtration of water for injection, LVPs, SVPs, tissue culture media final filtration of drugs and ...
Semantic Scholar extracted view of Effect of interferon on Sindbis virus growth in chick embryo cell cultures. by Robert B. Stewart et al.
Cell or tissue culture experimentations should not be carried out in the regular laboratory space where other laboratory investigations are undertaken. This is critical to avoid contamination of cells in the cell culture plates and also to ensure that all the physiochemical environmental factors that encourage optimal growth of the cells are provided. Thus, cell culture experimentations should be carried out in a specialized laboratory or an area in the regular laboratory that is secluded from the usual laboratory area in order to achieve optimal result. Some key environmental conditions (i.e. the physiochemical or physico-chemical environmental growth factors) must be met in order to achieve optimum cell/tissue culture technique.. The microenvironment in which cell culture technique is basically carried out is unique and quite different from the traditional microbial cultures that also occur in vitro in the sense that tissue culture support the growth of living cells or cultures derived from ...
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* found in: DMEM,High Glucose w/L-Glutamine and P, Graces Suppl Media (Hinks), Chicken Serum, Sterile Filt, Rabbit Serum, Non-Sterile, DMEM,high gluc w/..
We have now now documented a tissue culture model method in which managed NC differentiation to SNPCs can be induced. Even further characterization of how the
S100A4 (Human) ELISA Kit is used for the quantitative measurement of human S100A4 in cell extract, tissue culture medium and other biological media. (KA2418) - Products - Abnova
Fibronectin, human, used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types.
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Overview and Resources: The Tissue Culture function has been in place since the original grant in 1997, although it has expanded considerably in response to cha...
Jeeven BioSciences, Inc. Announces the Release of its Neuro-PURE™ Serum Free and Xeno Free Tissue Culture Media. Neuro-PURE™ is the only commercially available 100% Serum Free and Xeno Free Tissue Culture Media on the Market.. Atlanta, Georgia (October 22, 2013) - Jeevan BioSciences, Inc. ( a start-up biotechnology company co-founded by Dr. Rodney Nash, PhD, a former Neuroscience Post-Doctoral Fellow at Emory University, today announces the commercial release of its first product Neuro - PURETM Tissue Culture Media; the first 100% Serum Free and Xeno Free media product available on the market. Neuro-PURETM is a proprietary patent pending chemically defined media specifically formulated for use in the growth of human cells and tissue without the need for serum or other animal products. The product was originally developed to support the growth of neuronal cells, but has been engineered to support the growth of multiple types of human cells including U87, HeLa, Neural ...
Organ cultures are practical tools to investigate regenerative strategies for the intervertebral disc. However, most existing organ culture systems induce severe tissue degradation with only limited representation of the in vivo processes. The objective of this study was to develop a space- and cost-efficient tissue culture model, which represents degenerative processes of the nucleus pulposus (NP). Intact bovine NPs were cultured in a previously developed system using Dyneema jackets. Degenerative changes in the NP tissue were induced either by the direct injection of chondroitinase ABC (1-20 U/mL) or by the diffusion of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) (both 100 ng/mL) from the culture media. Extracellular matrix composition (collagens, proteoglycans, water, and DNA) and the expression of inflammatory and catabolic genes were analyzed. The anti-inflammatory and anti-catabolic compound epigallocatechin 3-gallate (EGCG, 10 µM) was employed to assess the relevance of
In recent years, advances in plant genetic engineering have opened new avenues for crop improvement and various plants with novel agronomic traits have been produced. The success of plant genetic engineering relies on several factors which include an efficient tissue culture system, for regeneration of plants from cultured cells and tissues (Pua et al. 1996). Shoot generation and rooting are important in the realization of the potential of the cell and tissue culture techniques for plant improvement (Purnhauser et al. 1987). Silver ions in the form of nitrate, such as AgNO3, play a major role in influencing somatic embryogenesis, shoot formation and efficient root formation which are the prerequisites for successful genetic transformation (Bais et al. 1999; Bais et al. 2000a; Bais et al. 2000b; Bais et al. 2001a; Bais et al. 2001b; Bais et al. 2001c). Silver ions are also employed in the form of silver thiosulphate in several tissue culture studies (Eapen and George, 1997).. Ethylene is ...
Some of the compounds in the active fraction of ultrafiltrates of chick embryo extract have been identified as taurine, serine, glutamic acid, xanthine, uracil, glucose-6-phosphate, glucose, ferrous iron, and inorganic phosphate.. Based on the identity of these compounds a synthetic replacement for the ultrafilterable portion of chick embryo extract has been devised. There is an additional nutritional requirement that can be met by vitamin B12. Folic acid appears to be beneficial to the system though the requirements of this or any of the above compounds except vitamin B12 remain for future research. The low nitrogen content of the isolated fraction and the synthetic mixture suggests that the main nutrition of chick cells in roller tube cultures is derived from the non-dialyzable portion of the medium.. ...
Caisson Labs is a premier supplier of cell culture media, fetal bovine serum and other reagents for animal cell culture, as well as tissue culture media, gelling agents, hormones, and other biochemicals for plant tissue culture.
Caisson Labs is a premier supplier of cell culture media, fetal bovine serum and other reagents for animal cell culture, as well as tissue culture media, gelling agents, hormones, and other biochemicals for plant tissue culture.
An ASU Biodesign Institute team has reported their latest advancement in 3-D intestinal model development. The new study, a collaboration between Arizona State University and NASAs Johnson Space Center, was led by Cheryl Nickerson, a researcher at ASUs Biodesign Institute and professor in the School of Life Sciences. Their united goal is to develop more realistic models of intestinal tissue to thwart Salmonella, a leading cause of food poisoning and systemic disease worldwide with many varieties causing severe and sometimes fatal infections with an economic impact in the billions of dollars.
My research program is directed toward 1) pistachio genetics and breeding and 2) study of species relationships genetic identity and diversity of germplasm of pistachio, walnut, stone fruits, and several other crops. Research is being carried out on the extent and nature of genetic variation in these crops as measured with markers such as isozymes and RFLPs. Research is also being conducted on improved procedures for pistachio improvement, including the use of tissue culture techniques for rootstock improvement. Recent research has included evaluation of species relationships using cpDIVA variation and RFLPs. ...
Dehydrated tissue culture media, microbiology, microbiological media, culture media, microbiology selective, enriched media, agar preparation, microbiology types, plant tissue, microbiological medium, microbial media, media bacteria, bacterial media, microbiological culture, mixed culture microbiology, complex media microbiology, autoclave microbiology, plant tissue culture media
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OA Text is an independent open-access scientific publisher showcases innovative research and ideas aimed at improving health by linking research and practice to the benefit of society.
Quasi Vivo Training - Theory and practice of advanced cell culture techniques through a combination of lectures, demonstrations and practical experience.
Hiramoto, R; Jurandowski, J; Bernecky, J; and Pressman, D, Immunohistochemical identification of tissue culture cells. (1961). Subject Strain Bibliography 1961. 1057 ...
Tissue culture is a technique of developing an organ or tissue in a laboratory using fragments of tissues and organs, on a nutrient media for proper development.
Tissue Culture Reagents market aims to decode lucrative investment opportunities in the business sphere and educate readers about the bottlenecks created by Covid-19 pandemic.
Tissue culture mixer - Modèle Cel-Gro (). Features Optional culture results! Available in single or dual drum configuration with adjustable drum angle Choose from fixed or adjustable speed 24 Month Warrant
tissue culture is the procedure to grow cells or tissues under totally controlled condition.but cell culture affected by contamination or microbial....
A procedure has been developed for the determination of the concentration of infective Newcastle disease virus (NDV) based on the enumeration of singly infected and distributed HeLa cells which are visualized by staining with fluorescent antibody. Infective virus assayed by the fluorescent cell-counting procedure is expressed in terms of cell-infecting units (CIU).. Adsorption of NDV to HeLa cell monolayers reached a plateau 1 to 1.5 hours after inoculation of coverslip cultures, and 12 per cent of the infective particles inoculated failed to adsorb. The half-life of NDV in protein-free Eagles medium at 37°C. was 2.1 hours. There was a linear relationship between virus concentration and the number of infected cells. The coefficient of variation of the mean of replicate determinations of infective NDV was 8.2 per cent. The distribution of single infected HeLa cells in the monolayer corresponded to the Poisson distribution. With NDV the cell-infecting unit (CIU) determined in HeLa cells is ...
Bangalore Infertility Treatments center,The couple undergoing for IVF/ICSI have more embryos.Blastocyst Culture,Technique in IVF to select the best embryo.
In 1972, Peter Mazur, Stanley Leibo, and Ernest Chu published, A Two-Factor Hypothesis of Freezing Injury: Evidence from Chinese Hamster Tissue-culture Cells, hereafter, A Two-Factor Hypothesis of Freezing Injury, in the journal, Experimental Cell Research. In the article, the authors uncover that exposure to high salt concentrations and the formation of ice crystals within cells are two factors that can harm cells during cryopreservation. Cryopreservation is the freezing of cells to preserve them for storage, study, or later use. Mazur originally suggested the two factors in a 1970 paper, but that article was based on evidence from simple yeast cells. By using hamster cells in 1972, Mazur, Leibo, and Chu confirmed that Mazurs two-factor hypothesis applied to more complex mammalian cells. The article dispelled the widely accepted notion that rapid cooling rates were safest for all cells, and instead showed that each kind of cell had a different optimal cooling rate depending on the solution ...
Tian, Y., Tarlow, O. and McCrae, Malcolm A. (1990) Nucleotide-sequence of gene-2 of the UK tissue-culture adapted strain of Bovine Rotavirus. Nucleic Acids Research, 18 (13). p. 4015 ...
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Nest BioFactory™ Systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications, such as production of vaccines, monoclonal antibodies or pharmaceuticals. Versatile, easy-to-use systems for small scale research or commercial production with reduced contamination risk. Narrow mouth caps available for tubing solutions. Available Vacuum plasma TC-Treated in five sizes with total cell growth area of 647, 1279, 2175, 6335 or 25295 cm2. BioFactory™ Systems are manufactured in strictly controlled ISO 8 / 100K Grade Clean Room to exacting manufacturing standards, and rigid QA procedure. Manufacturing is accomplished by use of state of the art robotics with automatic conveyers, hot runner molds, and vacuum plasma and corona treatment.
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
Blastocyst culture is for improved take home baby rate. But blastocyst culture may reduce the number of embryo transfers as all embryos may not grow to the blastocyst stage & may be arrested at 4 or 6 cell stage, thus resulting in cancellation of the entire cycle. Blastocyst culture. ...
Besides the intended effects that give a genetically modified (GM) plant the desired trait, unintended differences between GM and non-GM comparable plants may also occur. Profiling technologies allow their identification, and a number of examples demonstrating that unintended effects are limited and diverse have recently been reported. Both from the food safety aspect and for research purposes, it is important to discern unintended changes produced by the transgene and its expression from those that may be attributed to other factors. Here, we show differential expression of around 0.40% transcriptome between conventional rice var. Senia and Senia-afp constitutively expressing the AFP antifungal protein. Analysis of one-fifth of the regulated sequences showed that around 35% of the unintended effects could be attributed to the process used to produce GM plants, based on in vitro tissue culture techniques. A further ∼15% were event specific, and their regulation was attributed to host gene ...
TY - JOUR. T1 - Quantitative aspects of L triiodothyronine (T3) binding by rat liver and kidney cytosol. AU - Dillman, W.. AU - Surks, M. I.. AU - Oppenheimer, J. H.. PY - 1974/1/1. Y1 - 1974/1/1. UR - UR - M3 - Article. AN - SCOPUS:19244368397. VL - 22. SP - 338a. JO - Journal of Investigative Medicine. JF - Journal of Investigative Medicine. SN - 1081-5589. IS - 3. ER - ...
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A sensitive and accurate tissue culture system and kit fop detecting subtle changes in immune function is provided. The system is based on the comparison of IL-2 production by T helper cells in response to recall antigens including influenza A virus, tatanus toxoid, alloantigens, mouse xenogeneic antigens and the like or combinations thereof. Different stages of immune dysfunction can be differentiated and organ graft rejection can be predicted by the method of the present invention.
A new tissue culture model using human white blood cells shows how people with a latent -- or symptom-free -- tuberculosis infection are protected from active disease by a critical early step in their immune response, researchers say.
If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated. Virginia Tech , University Libraries , Contact Us ...
Researchers at the University of Chicago Medical Center have succeeded in growing HPV, the human papilloma virus-cause of genital warts and linked to carcinoma of the cervix-in cultured tissue for
This book provides an introduction to qualitative and quantitative aspects of human physiology. It looks at biological and physiological processes
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This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male.
Les outils agricoles à l'épreuve de l'open source". Techniques & Culture. Revue semestrielle d'anthropologie des techniques (in ...
Decker, Kris De (6 June 2017). "Comment bâtir un internet low tech". Techniques & Culture. Revue semestrielle d'anthropologie ... Different versions of Wi-Fi use different techniques, 802.11b uses DSSS on a single carrier, whereas 802.11a, Wi-Fi 4, 5 and 6 ... Mateti, Prabhaker (2005). "Hacking Techniques in Wireless Networks". Dayton, Ohio: Wright State University Department of ... or 4G networks using the tethering technique. Many smartphones have a built-in capability of this sort, including those based ...
Certain materials and techniques are commonly used and frequently favored by mail artists due to their availability, ... Stewart Home, The Assault On Culture, Aporia Press & Unpopular Books, London 1988[full citation needed] Hendricks, Jon (1988). ... Various printmaking techniques, in addition to rubber stamping, are used to create multiples. Copy art (xerography, photocopy) ... Mail artists often use collage techniques to produce original postcards, envelopes and work that may be transformed using copy ...
The technique nishijin-ori, traditionally produced in the Nishijin area of Kyoto, is intricately woven and can have a three ... ISBN 0-88011-598-X. Dalby, Liza (2001). Kimono, Fashioning Culture. Vintage. ISBN 0-09-942899-7. Fält, Olavi K.; Nieminen, Kai ... Bennett, Gary (1997). Aikido techniques & tactics. Human Kinetics Publisher. ... dye technique. Obidome (帯留, "sash clip") is a small, decorative brooch fastened onto the obijime at the front, commonly made ...
Techniques & Culture [Online]. Nugroho, Irawan Djoko (2011). Majapahit Peradaban Maritim. Suluh Nuswantara Bakti. ISBN ...
Robert Arthur Andersen (2005). Algal culturing techniques. Elsevier Academic Press. ISBN 9780120884261. Tao, S.H.; Bolger, P.M ... For this application, the concentration of germanium dioxide typically used in the culture medium is between 1 and 10 mg/l, ... Germanium dioxide is used in algaculture as an inhibitor of unwanted diatom growth in algal cultures, since contamination with ...
ISBN 90-316-0078-4. "Levkas". Google Arts & Culture. Retrieved 26 December 2018. CS1 maint: discouraged parameter (link) Bruno ... ISBN 978-88-404-4218-1. "Techniques and materials". British Museum. Retrieved 26 December 2018. CS1 maint: discouraged ...
"Historical review of algal culturing techniques". In Anderson, Robert A. (ed.). Algal Culturing Techniques. Elsevier Science & ... Tamiya was able with it to culture algal cell lines that were all in the same developmental stage, a technique used by later ... In 1953 Tamiya, working with other Japanese scientists, developed techniques for the synchronous culture of the green algae ... In 1977 he was given the Japanese Order of Culture for his contributions to science in Japan. The standard author abbreviation ...
Rubin, Emanuel; Baron, John H. (2006). Music in Jewish History and Culture. Sterling Heights, Michigan: Harmonie Park Press. ... Boskovich introduced him immediately to twentieth-century techniques and influenced him greatly as a composer and, eventually, ... ISBN 0-674-37471-1. Read, Gardner (1976). Contemporary Instrumental Techniques. New York: Schirmer Books. ISBN 9780028721002. ... extended and invented vocal and instrumental techniques, and required virtuosity and perseverance from performers. In fact, ...
Anie Montigny (2004). "La légende de May et Ghilân, mythe d'origine de la pêche des perles ?". Techniques & Culture (in French ...
Netanya: Association for Society and Culture. Association for Society and Culture - Netanya Labbe necklace. ... after its technique. Until then, most were prepared by using casting techniques, and embossing and fashioning from solid pieces ... Today, in modern techniques, they use either a thin metal wire which is melted or plate of same substance. The purpose of the ... 128-129 Van Praag, Esther (2007), p. 100 A modern technique of making a temporary binder (jeweler's cement) is to take 6 parts ...
Popular Culture and Philosophy. 57. Open Court Publishing Company. ISBN 9780812697162. Popoff, Martin (2017). Rush: Album by ... Technique. Hal Leonard Corporation. 2015. ISBN 9781495032714. Berti, Jim; Bowman, Durrell (2011). Reisch, George A. (ed.). Rush ...
Micropropagation techniques including tissue culture. Bulbils, which are adventitious bulbs formed on the parent plant's stem. ... Further species were collected from Persia and the spreading Ottoman Empire saw to it that tulip culture also spread. By the ... Bulb offsets and tissue culture produce genetic clones of the parent plant and thus maintaining genetic integrity of the ... Nishiuchi, Y (1986). "Multiplication of Tulip Bulb by Tissue Culture in vitro". Acta Hort. (ISHS). pp. 279-284. Archived from ...
Koh, J.; D, S.H.P. (2009). Culture and Customs of Singapore and Malaysia. Cultures and Customs of the World. ABC-CLIO. p. 100. ... Albala, K. (2017). Noodle Soup: Recipes, Techniques, Obsession. University of Illinois Press. p. pt198. ISBN 978-0-252-05019-0 ... Kraig, B.; D, C.T.S.P. (2013). Street Food around the World: An Encyclopedia of Food and Culture: An Encyclopedia of Food and ... They show a distinct blend of Oriental and Western culture adjusted to suit the Philippine palate. They are normally served ...
Grimaud, Emmanuel; Tastevin, Yann Philippe; Vidal, Denis (2017). "Low-tech ? Wild-Tech !". Techniques & Culture (in French) (67 ... Low-tech are no longer restricted to old techniques, but also extended to new, future-oriented techniques, more ecological and ... In some definition, low-tech techniques and designs may fall into disuse due to changing socio-economic conditions or ... the concept of low-tech is simply defined as a technique that is not recent, or using old materials. Companies that are ...
Culture techniques (with Neubert, eds.), Walter de Gruyter- Verlag, Berlin, 1981. Methods in prenatal toxicology (with Neubert ...
Two Bits Man (2007-01-19). "Two Bits shares an attempt at lyrical poetry about Funkmasta G. Wayne". The Technique. Archived ... Stolberg, Sheryl Gay; Taylor, Kate (2011-04-03). "Wounded in Crossfire of a Capital Culture War". The New York Times. Retrieved ... Lange, Scott (1998-04-17). "Board of Regents gives IAC restructuring a nod". The Technique. Archived from the original on 2008- ... Shaw, Jody (2002-10-11). "State of the Institute from one student's perspective". The Technique. Archived from the original on ...
1996 Member of the Advisory Board of Culture magazine, Ministry of Culture. - 1998 Director General (Founder) of the Office of ... The technique features of Playwriting in Yemen, Academy of Arts Cairo 2006. • In addition to a large number of studies ... The Ministry of Culture awarded him the honorary shield for the pioneers of the Yemeni theater. In addition to a cash prize in ... He was also responsible for editing art magazines and literary magazines in the Ministry of Culture in Yemen. His biography was ...
1982). Media analysis techniques. Beverly Hills: Sage Publications. (1984). Signs in contemporary culture: An introduction to ... 2011). Ads, Fads, and Consumer Culture: Advertising's Impact on American Character and Society. Boulder, Colo: Rowman & ... Walnut Creek, California: AltaMira Press (1998). Media research techniques. Thousand Oaks: Sage Publications. (2003). Durkheim ... Asa Berger Professor Emeritus Broadcast and Electronic Communication Arts San Francisco State University.The Popular Culture ...
Baskett, Michael (2009). "All Beautiful Fascists? Axis Film Culture in Imperial Japan" in The Culture of Japanese Fascismed. ... a traditional Japanese technique for dealing with the unacceptable. Most propaganda attacks against the American troops were ... The Shinmin no Michi or Path of Subjects described what the Japanese should aspire to be, and depicted Western culture as ... The risk of alienating the same cultures that the Japanese ostensibly were "liberating" from the yoke of Western colonial ...
"Sex Technique". Archived from the original on 21 July 2015. Retrieved 27 July 2015. Suad, Joseph (2006). ... 98-108 Marc Baer, "Death in the Hippodrome: Sexual Politics and Legal Culture in the Reign of Mehmed IV," Past & Present 210, ... 7-31 Haideh Moghissi (2005), Women and Islam: Part 4 Women, sexuality and sexual politics in Islamic cultures, Taylor & Francis ... Stephen O. Murray and Will Roscoe (1997), Islamic Homosexualities: Culture, History, and Literature, ISBN 978-0814774687, New ...
ISBN 0-07-241424-3.CS1 maint: ref duplicates default (link) Kahn-Harris, Keith (2007). Extreme Metal: Music and Culture on the ... According to former Metal Maniacs editor Jeff Wagner, the band's songwriting techniques peaked with the fourth album, Rust in ... ISBN 978-0-85712-117-2.CS1 maint: ref duplicates default (link) Tarquin, Brian (2012). Recording Techniques of the Guitar ... ISBN 978-1-4354-6017-1.CS1 maint: ref duplicates default (link) Thompson, Graham (2007). American culture in the 1980s. ...
... : Images and Techniques. 1996 Marg Publications edited by Gayatri Sinha: (Chapter in) Expressions and ... "Anjolie Ela Menon". INDIAN CULTURE. Retrieved 14 March 2021. "Anjolie Ela Menon , Indian painter". Encyclopedia Britannica. ... and nudes that incorporated a vibrant colour palette and were rendered in a variety of styles ranging from cubism to techniques ...
A. Adair; M.L. Downie; S. McGreal; G. Vos (2013). European Valuation Practice: Theory and Techniques. Taylor & Francis. p. 215 ... Handbook of the Economics of Art and Culture (PDF). 1. Elsevier. p. 199. doi:10.1016/S1574-0676(06)01006-4. v t e v t e. ...
... techniques of mediation and mental transformation; and contemporary and ancient arts and culture. These are presented to the ... Part of a worldwide network of Tibet Houses, Tibet House US focuses on the promotion and preservation of Tibetan culture ... THUS works to preserves Tibet's intangible culture heritage when it is threatened inside and outside Tibet, and presents a ... Sisario, Ben (February 24, 2010). "Concert Still Shines a Light on Tibetan Culture". The New York Times. Retrieved 1 April 2019 ...
Ward, Charles (21 May 1989). "Comic-strip swipe at American culture: Where's Dick? employs popular music, novel storytelling ... techniques". Houston Chronicle. Retrieved 21 September 2012. Where's Dick? at (audio samples and ...
"Techniques for Obtaining Rank Orderings". With: J. David Mart. In: Sociological Methods & Research, Vol. 1, No. 3, 317-327. ... A Scientific Foundation for World Culture. With Burt Webb. * Otto N. Larsen (1976). "In Memoriam: Stuart Carter Dodd, 1900-1975 ...
The maturation technique is where the cultivation method choice is made. In one method the spat or seed oysters are distributed ... Oyster culture using tiles as cultch. Taken from The Illustrated London News 1881 Purpose made oyster baskets Working on ... This latter cultivation technique may be the least susceptible to predators and poaching, but is the most expensive to build ... After the fertilized eggs and beginning-stage larvae have been added to the tank, they should be fed filtered or cultured algae ...
Turkish language and culture infused northern Morocco with new words, sartorial items, and consumption habits--including the ... p. 8. ISBN 978-0-313-37626-9. "Pastilla Recipe - Bastilla Recipe - Delicious Techniques". Abitbol, Vera (2016-11 ... Albala, Ken (2011-05-25). Food Cultures of the World Encyclopedia [4 volumes]: [Four Volumes]. ABC-CLIO. ISBN 978-0-313-37627-6 ... ISBN 978-0-911974-07-2. Ken Albala (2011). Food Cultures of the World Encyclopedia. ABC-CLIO. ...
Anie Montigny (2004). "La légende de May et Ghilân, mythe d'origine de la pêche des perles ?". Techniques & Culture (in French ... In particular, the Ministry of Culture and Sports (MCS) and local universities have been at the forefront of these efforts to ... Like elsewhere on the Arabian Peninsula, folktales play an important role in Qatar's culture. Some of Qatar's folktales have a ... Government ministries such as the Ministry of Culture and Sports and local universities have made efforts to preserve and ...
Florence Tager, "A Radical Culture for Children of the Working Class: The Young Socialists' Magazine, 1908-1920," Curriculum ... It contains specimen lessons and technique help to the teachers together with suggested reading for socialist education. ... ...
New diagnostic techniques, such as trichoscopy may be used for non-invasive differential diagnosis of cicatricial alopecia. ... In addition, if pustules are present, cultures are taken to identify which microbes, if any, may be contributing to the ...
The issue of healthy eating has long been an important concern to individuals and cultures. Among other practices, fasting, ... dieting, and vegetarianism are all techniques employed by individuals and encouraged by societies to increase longevity and ... Eating positions vary according to the different regions of the world, as culture influences the way people eat their meals. ...
Traditional medicine refers to the pre-scientific practices of a certain culture, in contrast to what is typically practiced in ... Use of standard-of-care treatment in addition to an alternative technique being tested may produce confounded or difficult-to- ... Edzard Ernst characterized the evidence for many alternative techniques as weak, nonexistent, or negative[108] and in 2011 ... In Britain, the most often used alternative therapies were Alexander technique, Aromatherapy, Bach and other flower remedies, ...
In the case of conflict resolution, the tit-for-tat strategy is effective for several reasons: the technique is recognized as ... In Western business cultures, a handshake when meeting someone is a signal of initial cooperation. ... the tit-for-tat strategy has been of beneficial use to social psychologists and sociologists in studying effective techniques ...
In principle, practitioners of natural farming maintain that it is not a technique but a view, or a way of seeing ourselves as ... the methods themselves vary widely depending on culture and local conditions. ... Fukuoka's practice and philosophy emphasised small scale operation and challenged the need for mechanised farming techniques ... which Fukuoka considered to be another modern technique that disturbs nature.[9] ...
At the Ecole Technique he met Ieng Sary, who became a close friend and later a member of his government.[21] In summer 1949, ... Sâr made little or no attempt to assimilate into French culture[46] and was never completely at ease in the French language.[41 ... he enrolled in 1948 to study carpentry at the Ecole Technique in Russey Keo, in Phnom Penh's northern suburbs.[29] This drop ... which in Cambodian culture was traditionally associated with monkhood.[256] ...
B. C. Giovanella u. a.: Development of invasive tumors in the nude mouse after injection of cultured human melanoma cells. In: ... Occurrence of autoimmunity after xenothymus transplantation in T-cell-deficient mice depends on the thymus transplant technique ... D. Kong u. a.: Establishment and characterization of human pancreatic adenocarcinoma cell line in tissue culture and the nude ...
Society and culture. See also: Cultural effects of the Ebola crisis. Weaponisation. Ebolavirus is classified as a biosafety ... Education of caregivers in these techniques, and providing such barrier-separation supplies has been a priority of Doctors ... Hewlett, Barry; Hewlett, Bonnie (2007). Ebola, Culture and Politics: The Anthropology of an Emerging Disease. Cengage Learning ... Researchers looking at slides of cultures of cells that make monoclonal antibodies. These are grown in a lab and the ...
... contrasted with the knowledge of bronze making techniques in the Indus Valley cultures, calls into question the validity of ... Culture and economy[edit]. The Indus Valley civilisation was mainly an urban culture sustained by surplus agricultural ... Harappan Culture. Retrieved 13 January 2006. *^ [1] Poultry: Identification, Fabrication, Utilization by Thomas Schneller - ... The Harappan Civilisation has its earliest roots in cultures such as that of Mehrgarh, approximately 6000 BC. The two greatest ...
With basic Earth-surface based telescope and some technique, the size of the coma can be calculated.[12] Called the drift ...
... that was more elaborate than previous Acheulean techniques.[4] This technique increased efficiency by allowing the creation of ... Upper Paleolithic cultures appear to have had significant knowledge about plants and herbs and may have, albeit very rarely, ... Middle Paleolithic stone tool manufacturing spawned a tool making technique known as the prepared-core technique, ... "Intro to archeology The First People and Culture". Introduction to archeology. Retrieved 2008-03-20.. ...
Other packaging techniques for discrete transistors as chips include Direct Chip Attach (DCA) and Chip On Board (COB).[96] ... "groundbreaking invention that transformed life and culture around the world".[62] Its importance in today's society rests on ...
Society and culture[edit]. The costs and social impact of acne are substantial. In the United States, acne vulgaris is ... The Leeds acne grading technique counts acne lesions on the face, back, and chest and categorizes them as inflammatory or non- ... The latter is thought to be primarily attributable to improper technique by the practitioner, including the use of excessive ...
Saraswati, Swami Satyananda (January 2004). A Systematic Course in the Ancient Tantric Techniques of Yoga and Kriya. Nesma ... "head technique"),[5] while the 18th century Joga Pradīpikā calls it Kapālī āsana, head posture; it is number 17 of the set of ... Physical culture/yoga *B. C. Ghosh. *K. V. Iyer. *Seetharaman Sundaram. *Paramahansa Yogananda *Autobiography of a Yogi ...
Oral swabs are taken if culture is required. Some recommend that swabs be taken from 3 different oral sites. Oral rinse ... Oral rinse technique can distinguish between commensal candidal carriage and candidiasis. If candidal leukoplakia is suspected ... With more sensitive detection techniques, this figure is reported to rise to 90%. This candidal carrier state is not considered ...
Newer techniques using ex-vivo expansion of cord blood units or the use of two cord blood units from different donors allow ... during which neither showed traces of HIV in their blood plasma and purified CD4 T cells using a sensitive culture method (less ... The technique is referred to as a bone marrow harvest and is performed under general anesthesia. ...
Technoscience and Environmental Justice: Expert Cultures in a Grassroots Movement. MIT Press. pp. 1-18. ISBN 978-0262015790.. ... Technology can be the knowledge of techniques, processes, and the like, or it can be embedded in machines to allow for ... Hughes, Thomas P. (2004). Human-Built World: How to Think About Technology and Culture. Chicago: University of Chicago Press. ... Technoscience and Environmental Justice: Expert Cultures in a Grassroots Movement. MIT Press. pp. 229-48. ISBN 978-0262015790. ...
End-user development, a technique that allows people who are not professional developers to perform programming tasks, i.e. to ... 1% rule (Internet culture). *Anonymous post. *Prosumer. *Pseudonym. *End-user computing, systems in which non-programmers can ... It designates activities or techniques in which people who are not professional developers create automated behavior and ...
"Polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique". Indian Journal of Medical Microbiology. 32 ... In a Dalmau plate culture containing cornmeal agar and Polysorbate 80, K. marxianus forms a rudimentary to branched ...
Comics As a Nexus of Cultures: Essays on the Interplay of Media, Disciplines. McFarland. p. 203. ISBN 978-0-7864-3987-4.. ... Kassim, I.; Phee, L.; Ng, W. S.; Gong, F.; Dario, P.; Mosse, C. A. (2006). "Locomotion techniques for robotic colonoscopy". ... Forsythe, J. W.; Hanlon, R. T. (1980). "A closed marine culture system for rearing Octopus joubini and other large-egged ... Octopus is eaten in many cultures and is a common food on the Mediterranean and Asian coasts.[141][142] The arms and sometimes ...
The collaboration of people were in a group called PACT (The Project for the Advancement of Coding techniques). After passing ... Some research and interviews with members of open-source projects have described a male-dominated culture within open-source ... and stewardship organization at a cusp moment in the history of that culture."[6] ... "Introduction: Open source culture and aesthetics". Criticism. 53 (3): 337. doi:10.1353/crt.2011.0026.. [permanent dead link ...
75% of today's sake is made using this technique, left over from the war years. There were even a few breweries producing "sake ... An Indispensable Guide to Sake and Japanese Culture. *What Does Sake Taste Like? ... the technique of distillation was introduced into the Kyushu district from Ryukyu.[5] The brewing of shōchū, called "Imo-sake" ...
Folk medicine consists of the healing practices and ideas of body physiology and health preservation known to some in a culture ... Since knowledge, techniques, and medical technology continue to evolve at a rapid rate, many regulatory authorities require ... "Assessing patients effectively: Here's how to do the basic four techniques". Nursing2014. 8 (2): 6. 2006.. ... Becka J (1980). "The father of medicine, Avicenna, in our science and culture: Abu Ali ibn Sina (980-1037) (Czech title: Otec ...
Craftsmen passed their skills and techniques on from one generation to the next. They used hand, horse, wind, and water power, ... German-American culture in Iowa. *Radical Pietism. *History of Buffalo, New York ...
The History and Culture of Iroquois Diplomacy: An Interdisciplinary Guide to the Treaties of the Six Nations and Their League. ... They developed sophisticated techniques of hunting and managing their resources.. According to Dutch settler Isaac de Rasieres ... Carman, Alan, E. Footprints in Time: A History and Ethnology of The Lenape-Delaware Indian Culture. Trafford Publishing, 2013. ... Harrington, Mark R. Vestiges of Material Culture Among the Canadian Delawares. New Era Printing Company, 1908. ASIN B0008AV2JU. ...
Andrew F. Smith (October 1994). The tomato in America: early history, culture, and cookery. University of South Carolina. p. 14 ... Agrobacterium-mediated genetic engineering techniques were developed in the late 1980s that could successfully transfer genetic ...
Algorithms and techniques used to attain color constancy are frequently used for color balancing, as well. Color constancy is, ...
Other research suggests alternative techniques to stimulating neurons in vitro. One study investigates the use of a laser beam ... A cultured neuronal network is a cell culture of neurons that is used as a model to study the central nervous system, ... Like most cell cultures, neuron cultures are highly susceptible to infection. They are also susceptible to hyperosmolality from ... Cultured neuronal networks are by definition disembodied cultures of neurons. Thus by being outside their natural environment, ...
Folk long song performance technique of Limbe (flute) performances - circular breathing 2011 [22]. ... "UNESCO Culture Sector. Retrieved 2011-02-03.. *^ UNESCO has inscribed falconry as a shared intangible heritage element of ... The culture of Mongolia has been heavily influenced by the Mongol nomadic way of life. ... "UNESCO Culture Sector. Archived from the original on October 7, 2012. Retrieved 2009-09-07.. ...
Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been ... Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been ... Bridgen M.P., Van Houtven W., Eeckhaut T. (2018) Plant Tissue Culture Techniques for Breeding. In: Van Huylenbroeck J. (eds) ... Embryo culture Micropropagation Plant tissue culture Protoplast Somaclonal variation This is a preview of subscription content ...
... systems and processes that play into a companys agenda are what determine its culture -- and ultimately, its bottom line. ... Culture is different from climate. Culture refers to how things get done whereas climate describes what its like to work for ... Culture is key to the success of your business as it represents its brand, strategy and execution. Identify the habits that ... Organizational culture is comprised of consistent practices that build into a companys cadence. Every business has a cadence ...
... this publication presents the technique, tradition, context and production of some 30 different kinds of objects, focusing on ... culture_of_wood.html?id=3_lTAAAAMAAJ&utm_source=gb-gplus-shareJapan--culture of wood. ... sliding doors slightly smoothed snap lines split spoon staves strips structure surface Suzuki Tanaka tatami teahouse techniques ... this publication presents the technique, tradition, context and production of some 30 different kinds of objects, focusing on ...
ECACC cell culture handbook provides fundamental cell culture techniques and protocols used to successfully thaw, expand and ... we have assembled this updated laboratory handbook of cell culture techniques. For the researcher new to cell culture, this ... The European Collection of Authenticated Cell Cultures (ECACC) was established in 1985 as a cell culture collection to service ... Protocols for the use of induced pluripotent stem cells and for growing cells in 3D cell culture, two areas of growing ...
Keywords: MODS; new diagnostics; thin-layer agar; tuberculosis culture; tuberculosis diagnosis Document Type: Research Article ... rapid microcolony-based culture methods.. METHODS: A systematic review and meta-analysis was performed to assess the accuracy ... and other test characteristics of MODS and TLA compared to a reference standard of traditional solid or liquid culture. Pooled ...
A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering, Isolation and Culture of ... culture techniques include Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow, ... Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model, Endothelialized Microfluidics for ... Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice, Evaporation-reducing Culture Condition ...
For this Ask the Expert Session from The Cell Culture Dish, we have assembled a team of experts to answer your questions on ... Many tools and techniques have been developed for organoid culture, however depending on the cell type, research area and ... Using Organoids for Disease Modeling , Organoid Culture Tools and Techniques , Corning. We use cookies to ensure the best ... In general, cell culture products used for 2D are less advanced than products offered for 3D cell culture and therefore, the ...
Urine culturing technique in febrile infants.. Bonadio WA.. Abstract. During the period from July to November 1984, 265 ... The technique utilized for collecting urine for culture in infants has a major impact on the incidence of urinary tract ... Eighty-seven percent of infants with a positive urine culture result collected by bag technique revealed a mixture of more than ... Over the next four months, catheterization and suprapubic aspiration techniques increased from 0 to 72%; bag technique ...
tags: techniques x neuroscience x culture x evolution x The Scientist. » techniques, neuroscience, culture and evolution ...
tags: techniques x immunology x culture x The Scientist. » techniques, immunology and culture ...
These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor ... Recent studies have demonstrated the potential of utilizing 3D cell culture models in drug discovery programs; however, it is ... Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely ... a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two- ...
Contents: Genetic variation from in vitro culture; Genetic stability of in vitro cultures; In vitro culture with application of ... In vitro culture techniques considerably accelerate the plant breeding cycle. This factor and the advantage of using haploids ... Nuclear Techniques and in Vitro Culture for Plant Improvement (Vienna, 19-23 Aug. 1985). ... Nuclear Techniques and in Vitro Culture for Plant Improvement (Vienna, 19-23 Aug. 1985) ...
... labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques. ... The term tissue culture arose because most of the early cells were derived from primary tissue explants, a technique that ... Serious attempts at the culture of whole tissues and isolated cells were first undertaken in the early 1900s as a technique for ... This initial organ culture was used to establish several distinct astroglial cell lines. The C8-B4 clone was created in 1996, ...
... posted in General Lab Techniques: Hi; I am new to research world and would like to learn more about human lymphoblasts culture ... techniques, and general rules used in tissue culture. Can someone direct me to good book / articles from where to start? I ... I am new to research world and would like to learn more about human lymphoblasts culture techniques, and general rules used in ... A good starting point for cell culture (not lymphocytes specfically) is R.I. Freshneys Culture of Animal Cells: a laboratory ...
... environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they ... Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has ... the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner ... Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do ...
Cell Culture Techniques · Cell Differentiation · Cells, Cultured · Coculture Techniques · Humans · Intervertebral Disk · Stem ... sandwich culture · Animals · Biological Transport, Active · Cell Culture Techniques · Cell Survival · Cells, Cultured · ... Cell Culture Techniques · Cell Proliferation · Cell Survival · Cells, Cultured · Culture Media, Conditioned · ... Amides · Capillaries · Cell Culture Techniques · Cell Movement · Cells, Cultured · Cytoskeleton · Endothelial Growth Factors · ...
"Organ Culture Techniques" by people in Harvard Catalyst Profiles by year, and whether "Organ Culture Techniques" was a major or ... A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated ... "Organ Culture Techniques" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Organ Culture Techniques" by people in Profiles. ...
Alternatives to commercially available cell culture insert membranes and manufacturing techniques. Agency:. Department of ... The use of these more complex 3D cellular models has driven the use of more complex microtiter plates, namely cell culture ... Equally important is to identify manufacturing techniques that allow for the attachment of these custom membranes to CCIPs in a ... To overcome this limitation researchers at NIH have developed a technique that utilizes a biocompatible adhesive to attach an ...
Automated quantification of cell density within culture vessels enables routine passaging of cells at the optimal density and ... Reproducible cell-based assays require consistent maintenance of cell cultures and accurate evaluation of culture ... provides valuable information for conducting cell culture quality control studies. ... Cell Culture QC Automated imaging tools can provide valuable information for improving routine cell culturing techniques and ...
Use of cell separation and short-term culture techniques to study erythroid cell development.. Glass J, Lavidor LM, Robinson SH ... These techniques have provided a model system for the study of erythroid cells at different stages of maturation isolated from ... In short-term culture, erythropoietin stimulated proliferation of pronormoblasts and basophilic normoblasts but probably not ... and separation of the residual nucleated erythroid cells as a function of size by the velocity sedimentation technique. The ...
Production de paysage et culture technique. Lemploi de la céramique architecturale au Québec, du XVIIe au XXe siècle (note de ... recherche). An article from journal Anthropologie et Sociétés (Des systèmes techniques), on Érudit. ... Production de paysage et culture technique. Lemploi de la céramique architecturale au Québec, du XVIIe au XXe siècle (note de ... "Production de paysage et culture technique. Lemploi de la céramique architecturale au Québec, du XVIIe au XXe siècle (note de ...
... the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • ... 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media ... 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media ... Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 ...
Vital Techniques are the versatile bass duo behind the label Second To None Music. With a number of huge releases under their ... Vital Techniques. Vital Techniques are the versatile bass duo behind the label Second To None Music. With a number of huge ...
TCC techniques: 7. Cell viability and cell density calculations. 12. TCC techniques: 8. Basics for preparing suspension culture ... Cell Culture Techniques (CEAC514) Course Detail. Course Name. Course Code. Season. Lecture Hours. Application Hours. Lab Hours ... TCC techniques: 9. Basics for preparing adherent culture of cell lines from frozen stocks. ... Basic cell culture techniques for bioscience research and commercial applications. introduction to cell cultivation methods, ...
Top : Forum Archives: : General Lab Techniques. Freshness of a culture for staining purposes - (Dec/11/2007 ). I wish to do ... ie - will there be breakdown of the culture and production of substances that you wouldnt find in a fresh culture - do you ... Do I culture on agar or broth for this purpose? I have a few suggestion using other media but Ill be using LB (agar and broth ... wont a fresh culture means a good nutritional environment hence absence of spores?. Thanks people. ... explores the history of the craft. ... explores the history of the craft.. The first ... Primitive printing techniques involving the use of potato, turnip and beetroot stamps gave way to more durable wooden stamps ... However, one of the most typical medieval prints is signillis ornatae, a powder-based technique using silver and gold that was ... Their clothing and the technique used to adorn it was a visual symbol of the financial standing of its wearer. ...
The centrifugation technique recovered 73% of the positive cultures. The broth and pour plate techniques recovered 38 and 49%, ... Blood culture technique based on centrifugation: clinical evaluation.. G L Dorn, G G Burson, J R Haynes ... Blood culture technique based on centrifugation: clinical evaluation. Message Subject (Your Name) has forwarded a page to you ... for each organism the time required for the detection of a positive culture was shortest for the centrifugation technique. ...
Clarke, W. J.; Palotay, J. L. & Mahlum, D. D. The Use of Tissue Culture and Cytochemical Technique in Toxicologic Research, ...
Unless indicated otherwise, the text in this article is either based on Wikipedia article "Twelve-tone technique" or another ... Twelve-tone technique (also dodecaphony and, especially in British usage, twelve-note composition) is a method of musical ... The technique is a means of ensuring that all 12 notes of the chromatic scale are sounded as often as one another in a piece of ... The technique was tremendously influential on composers in the mid-20th century. ...
  • This rate of biodegradation is a key factor for certain tissue model types where an initial scaffold is necessary to promote structural integrity, cellular anchorage, viability and proliferation but where over time the desire is for the scaffold to degrade and be replaced by naturally produced ECM and promote cell to cell interaction in co-culture models. (
  • After 48 h of culturing in these devices the cell viability of 79 [plus or minus] 6 % (n = 3). (
  • Viability and function in lymphocytes cultured from the horse, chicken, and mouse: effects of different leukocyte enrichment techniques. (
  • In this study, we assessed lymphocyte viability and proliferation of avian, equine, and murine lymphocytes enriched by a commercial density-gradient technique and under identical, standardized culture conditions. (
  • Students will become familiar with common techniques such as trypsinizing cells, cell counting with a hemocytometer, performing serial dilutions, and determining cell viability using trypan blue dye. (
  • In the first approach, gas chromatography-negative chemical ionization/mass spectrometry (GC-NCI/MS) of the thiohydantoin sample subsequent to the modified Edman degradation was performed using a thermodesorption/cold trap (TCT) injection technique (detection limit for in vitro exposure of human blood to sulfur mustard: 30 nM). (
  • In general, for each organism the time required for the detection of a positive culture was shortest for the centrifugation technique. (
  • Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens. (
  • Our data indicate that the shell vial cell culture assay for the detection of CMV is as specific as and more sensitive than conventional tube cell culturing for the diagnosis of CMV infections. (
  • Diagnosis is based on a combination of clinical risks, symptoms and signs, culture, histopathology and detection of the fungal components such as the antigen galactomannan and direct surface analysis bymatrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI0) [ 8 ]. (
  • Prevalence of Strongyloides stercoralis infection in northeastern Thailand (agar plate culture detection). (
  • Rapid detection of extended-spectrum {beta}-lactamase-producing Gram-negative bacilli in blood cultures. (
  • Objectives Conventional detection of extended-spectrum β-lactamase (ESBL) producers in blood cultures usually requires overnight incubation. (
  • A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi. (
  • The detection of bacteremia and fungemia by culturing blood remains one of the most important roles of the microbiology laboratory. (
  • The clinical urine culture: enhanced techniques improve detection of clinically relevant microorganisms. (
  • The microscopic observation drug susceptibility (MODS) assay and the thin-layer agar (TLA) assay are inexpensive, rapid microcolony-based culture methods. (
  • Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points. (
  • Additional specimens from 18 patients were positive in the shell vial assay but negative in the conventional tube cell culture assay. (
  • Recent innovations in human cell culture exposure and test systems has allowed the development ofin vitro assay systems that are predictive, representative and suitable for toxicity screening of a diverse range of chemicals including airborne materials and nanomaterials (NMs). (
  • The test has a simple procedure that combines parasite culture with a drug assay. (
  • In: Davey RM, Anthony P (eds) Plant cell culture: essential methods. (
  • METHODS: A systematic review and meta-analysis was performed to assess the accuracy and other test characteristics of MODS and TLA compared to a reference standard of traditional solid or liquid culture. (
  • This fluorescence image gallery explores over 30 of the most common cell lines, labeled with a variety of fluorophores using both traditional staining methods as well as immunofluorescence techniques. (
  • the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. (
  • There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models. (
  • The new technique is more than three times as effective as previous methods ( 'Expansion of CTCs from early stage lung cancer patients using a microfluidic co-culture model' ). (
  • The emergence of novel culture-independent methods, including targeted point-of-care and unbiased-metagenomic sequencing, are likely to become increasingly relevant. (
  • Carrel's long investigation in improving tissue culture methods was thus initiated. (
  • In this study three methods are compared for reporting blood culture results: a manual method that included use of a serum separator. (
  • Fourteen samples (9.5%) were culture-positive using both methods, and 73 (49.7%) were culture-negative using both techniques. (
  • HPTLC methods development using instrumental techniques as OPLC (Over Pressure Liquid Chromatography) and AMD (Automated Multiple Development) has been also described, as to simultaneously monitor several samples in the same elution, with significant time and solvent savings. (
  • Methods of lymphocyte enrichment tend to vary across species, with the most common techniques employed being density-gradient separation and erythrocyte lysis buffer enrichment. (
  • Duquenne B, Eeckhaut T, Werbrouck S, Van Huylenbroeck J (2007) Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium . (
  • The centrifugation technique showed an increased isolation rate for Pseudomonas, fungi, and gram-positive cocci. (
  • In an effort to follow the dynamics of mucosal colonization by H. parasuis , we developed a technique that allows the isolation of organisms that colonize the tonsil and upper respiratory tract of swine. (
  • 1. Pijoan C, Morrison R, Hilley H. Dilution technique for isolation of Haemophilus from swine lungs collected at slaughter. (
  • The diagnosis was established by isolation of Cryptococcus neoformans using the Isolator blood culture lysis-centrifugation system. (
  • Blood culture is currently the gold standard for diagnosis of typhoid fever, but it is time-consuming and takes several days for isolation and identification of causative. (
  • Presently, Widal test, stool, blood and urine cultures are the most diagnostic means of confirming Salmonella infection in humans since they are based on the isolation, identification and demonstration of the presence of antibodies in the serum. (
  • There are several factors that make these CCIPs suitable for use in the creation of complex cellular models, including the ability to co-culture cells with or without cell-to-cell contact, allowing for either apical or basolateral feeding to promote cellular metabolic activities, and provide an anchorage point which is typically required for most mammalian cells to remain viable. (
  • This study reports a viable protocol for obtaining fungus-free tissue culture callus from V. aestivalis. (
  • However, after 12 h of culturing, no viable cells were found. (
  • Publications] Shiroyanagi Y, Yamato M, Yamazaki Y, Toma H, Okano T.: 'Urothelium regeneration using viable cultured urothelial cell sheets grafted on demucosalized gastric flaps'BJU int. In press. (
  • Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. (
  • For obtaining the primary cultures, the organ is removed in a sterile atmosphere so as SO make it free from bacteria and other foreign bodies. (
  • Plant tissue culture useful for production of identical sterile hybrid species. (
  • disinfectant used in plant tissue culture lab to sterile the metal base and stone base platform before starting the process. (
  • Seal to culture holder or containers and also keep sterile to them. (
  • For this reason, we have devised a laboratory module and method herein that familiarizes students with common cell culture procedures, without the use of a sterile hood or large cell culture facility. (
  • Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely characterizes the disease state have been undertaken by a number of laboratories around the world. (
  • These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor cells. (
  • In addition, a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two-dimensional monolayer conditions do not respond to cancer therapeutics/compounds in a similar manner. (
  • To study the effects of a specific group of hormones (all ligands of the steroid/retinoid/thyroid hormone receptor superfamily) on the angiogenic process in humans, we have used a model system in which human microvascular endothelial cells from foreskin (hMVEC) are cultured on top of a human fibrin matrix in the presence of basic fibroblast growth factor and tumor necrosis factor-α. (
  • The new capture and culture method changes this by providing a reliable way to get usable numbers of circulating tumor cells from even early-stage patients. (
  • A team of researchers at the Sylvester Comprehensive Cancer Center at the University of Miami Miller School of Medicine has developed a technique for growing ovarian cancer cell lines that mimic the original tumor in long-term cultures. (
  • The technique offers hope for quickly advancing precision medicine applications for a variety of cancers by enabling clinicians to test therapies on cells from a patient's own tumor in the laboratory, thus identifying the most targeted therapy for that patient. (
  • The key to Ince's research is his development of a mixture of essential nutrients in liquid form, commonly referred to as a "cell culture medium," that permits cells taken from a patient tumor to replicate in a Petri dish and become a permanent cell line faithful to the characteristics of the original tumor over a long period of time in the laboratory. (
  • Electron microscopy shows cultured CTC cells (circled in red) growing on the chip device used in the study. (
  • Reliable techniques for the living cell culture and correlative light and electron microscopy (EM) of meiotic pollen mother cells (PMCs) of Iris spuria, Allium triquetrum and Tradescantia flumenensis are described in detail. (
  • Each biopsy specimen was bisected, and 1 piece was processed for immunohistochemistry or electron microscopy while the other piece was dissected further for explant culture. (
  • The original technique was based on the fast multiplying of a large number of epithelial cells. (
  • The development of new bladder augmentation technique using cultured bladder epithelial cell sheets. (
  • In 1907 Harrison successfully carried out the first tissue culture using the hanging drop method , in which fragments of embryonic frog nerve tissue were grown in a clot of lymphatic fluid. (
  • Burrows successfully established tissue cultures of embryonic chick cells. (
  • While the second generation device was not capable of successfully culturing cells, it did demonstrate the importance of culturing under dynamic conditions which lead to next design. (
  • The purpose of this study was to examine whether intact urothelial cell sheets harvested from new temperature-responsive cell culture systems without proteolytic enzyme treatment (1) would be autografted successfully onto demucosalized gastric flaps in a dog model to promote improved regeneration of native-like urothelium. (
  • Living PMCs were successfully cultured in a slide chamber on agar/sucrose medium. (
  • Of note, mean inhibitory concentrations of cultured isolates increased by at least three times over placebo in all treatment arms. (
  • Concentrations of cells in phytoplankton cultures are generally higher than those found in nature. (
  • Microcolony culture techniques for tuberculosis diagnosis: a syst. (
  • The technique may also bring doctors closer to their goal of capturing cancer cells for diagnosis with a quick, non-invasive blood biopsy instead of the tissue biopsies that are currently used. (
  • Thus, exhalation air culture is an easily performed bedside tool for diagnosis of aspergillosis in ventilated patients. (
  • Comments on the study "Blood Culture Bottles are Superior to Lysis-Centrifugation Tubes for Bacteriological Diagnosis of Spontaneous Bacterial Peritonitis," by P. D. Siersema, S. De Marie, J. H. van Zeijl and colleagues. (
  • The aim of this study was to assess the comparison between the Widal test and culturing technique in diagnosis of salmonellosis. (
  • The degree of bacterial contamination of 75 urine samples collected by voluntary voiding, catheterization, and cystocentesis was studied in 25 clinically normal dogs (14 females and 11 males) to evaluate the suitability of quantitative urine culture for diagnosis or urinary tract infection. (
  • The first method takes advantage of the wide spread DC technique and allows to study individual colonies of a cell monolayer grown on waterpermeable filter supports. (
  • Find out more in our " Avoiding Cell Culture Contamination Using the Aseptic Technique " paper from Bioquell. (
  • The major topics are: maintaining mammalian cells in culture (aseptic technique, thawing, counting, expanding, freezing) and troubleshooting (rescuing sick cells and recognizing and dealing with contamination). (
  • Techniques - Plant tissue culture performed in laminar air flow cause of aseptic condition also required for tissue culture programme. (
  • Aliquots of BPW were then processed using two different selective enrichment and culture techniques. (
  • Genetic engineering would not be possible without the involvement of in vitro plant tissue culture regeneration procedures. (
  • Do I culture on agar or broth for this purpose? (
  • In the first technique (TBG-RV-XLT4) samples were incubated at 43 °C in tetrathionate broth and then Rappaport-Vassiliadis broth before plating on XLT4 agar. (
  • The second technique (SEL-XLD) involved incubation at 37 °C in selenite broth before plating on XLD agar. (
  • Other PMCs were fixed with glutaraldehyde and flat embedded using a modification of the agar sandwich technique of Mole-Bajer and Bajer (1968). (
  • Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures. (
  • We use molecular techniques to understand how the overall amino acid structure contributes to the function and regulation of AQPs. (
  • Dr. Ince and his colleagues have created a paradigm shift, by demonstrating the ability to grow the majority of human ovarian cancers in long-term culture," said Richard J. Cote, M.D., Professor and Joseph R. Coulter Chair of the Department of Pathology, professor of biochemistry and molecular biology, Chief of Pathology at Jackson Memorial Hospital, and Director of BioNIUM. (
  • This study extends the knowledge of biofilm consortia composition by analyzing large urinary catheter and stent sample sets using both molecular and culture techniques, including the widest dataset of catheter-related samples characterized by 16S rRNA amplicon Illumina sequencing. (
  • Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding. (
  • The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. (
  • In short-term culture, erythropoietin stimulated proliferation of pronormoblasts and basophilic normoblasts but probably not cells at later stages of differentiation. (
  • This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. (
  • In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture. (
  • The complete kit contains basal media and five supplements that support sustainable proliferation and long term culture of growing cells. (
  • Various microscopic techniques were used to study the dependency of photodynamically induced subcellular reactions on the metabolic state of cell cultures. (
  • Tissue culture is an important technique which is used to study the fundamental problems in cell biology. (
  • Undergraduate students learn about mammalian cell culture applications in introductory biology courses. (
  • Students are more likely to learn about cell culture using bacteria or yeast, as they are typically easier to grow, culture, and manipulate given the equipment, tools, and environment of most undergraduate biology laboratories. (
  • While the standard urine culture is the gold standard for diagnosing UTI, data suggest that bacteria are present in approximately 90% of standard urine cultures with no bacterial growth. (
  • The broth and pour plate techniques recovered 38 and 49%, respectively. (
  • A program was instituted on August 1, 1984 which encouraged the utilization of either bladder catheterization or suprapubic aspiration techniques, and discouraged bag collection technique for culturing urine. (
  • We have developed a reliable protocol for the serum-free dissociation and culture of spiral ganglion neurons from adult mice, an important animal model for patients with post-lingual hearing loss. (
  • Using IR-differential interference contrast, good visualization of neurons in biomembrane slice cultures has been achieved despite a negative optical effect of the biomembrane, but epifluorescence imaging requires removal of a Wollaston prism and the analyzer. (
  • Using optic fiber illumination at a shallow angle from the top of the slice culture, with or without additional illumination from the bottom, we obtained good cellular resolution of neurons in biomembrane slice cultures as well as in acute slices with an infrared-video camera. (
  • With this technique, we demonstrate visually guided whole-cell patch-clamp recording of Na - and K -currents as well as combination of whole-cell recording with fluorescence imaging of hippocampal and entorhinal cortex neurons in biomembrane slice cultures. (
  • Objective To improve the success of culturing olfactory neurons from human nasal mucosa by investigating the intranasal distribution of the olfactory epithelium and devising new techniques for growing human olfactory epithelium in vitro. (
  • However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. (
  • In contrast, the utilization of mammalian cells requires a dedicated biological safety cabinet and rigorous antiseptic techniques. (
  • Other specimens from 14 of the 18 patients yielded CMV in conventional tube cell cultures. (
  • Of the 4 patients from whom CMV was not recovered from other specimens by conventional tube cell culturing, all had evidence of recent CMV infections, as indicated by a fourfold or greater rise in antibody titer. (
  • Two hundred twenty seven suspected aspergillosis patients were screened for fungal infection using different conventional tools to evaluate different respiratory specimens sputum, bronchoalveolar lavage, plural fluid, blood, biopsy and ELISA technique for galactomannan and (1-3)-β-D-glucan antigens in serum and bronchoalveolar lavage. (
  • Four Aspergillus species ( A. niger, A. fumigatus, A. flavus and A. terreus ) were isolated and identified morphologically and genetically using the conventional diagnostic tools from suspected patients in all positive cultures. (
  • Through a long-time practice, it has been proved to be an important breakthrough both in the tube rapid plant propagation of modern biotechnology and in the conventional seedling-breeding technique. (
  • Comparison of the Lysis Centrifugation Method with the Conventional Blood Culture Method in Cases of Sepsis in a Tertiary Care Hospital. (
  • To introduce a human cell culture technique for investigating in-vitro behavior of primary epiretinal cells and membrane contraction of fibrocellular tissue surgically removed from eyes with idiopathic macular pucker. (
  • This in-vitro technique using traction application allows for culturing surgically removed epiretinal membranes from eyes with idiopathic macular pucker, demonstrating cell behavior and membrane contraction of primary human epiretinal cells. (
  • Ex Vivo Oculomotor Slice Culture from Embryonic GFP-Expressing Mice for Time-Lapse Imaging of Oculomotor Nerve Outgrowth. (
  • Conclusions Despite BIA recommendations, time-to-LP was delayed and culture-independent techniques such as serum/CSF PCR for bacterial pathogens were under-utilised, despite out-performing CSF culture. (
  • The PRIMACYT Short and Long term Cell Culture media are part of our serum free cell culture media program. (
  • CMV was isolated in standard tube cell cultures (average time, 9 days) from only 88 specimens, but there were no instances (with the exception of 2 blood specimens) in which CMV was recovered from tube cultures but not from shell vials. (
  • Using massive parallel sequencing as a reference method in selected specimens, culture combined with PCR-DGGE was shown to be an efficient and reliable tool for determining the composition of urinary catheter-related biofilms. (
  • Specimens were fixed on cell culture plastic using small entomological pins to apply horizontal stress to the tissue, and then transferred to standard cell culture conditions. (
  • In serial cultivation fragments of one culture are transferred to new media to form secondary cultures, which ensures that the growth of tissues can be maintained for longer periods. (
  • Carrel and Burrows called this prolonged culturing "reactivation and cultivation in series. (
  • Rapid cultivation by transfers of culture. (
  • Serious attempts at the culture of whole tissues and isolated cells were first undertaken in the early 1900s as a technique for investigating the behavior of animal cells in an isolated and highly controlled environment. (
  • A good starting point for cell culture (not lymphocytes specfically) is R.I. Freshney's Culture of Animal Cells: a laboratory manual. (
  • These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. (
  • The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. (
  • Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. (
  • Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications , Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. (
  • Because of the continuing expansion of cell culture, and to keep the bulk of Culture of Animal Cells to a reasonable size, some specialized protocols are presented as supplementary material online. (
  • Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications , Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. (
  • Organ Culture Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (
  • This graph shows the total number of publications written about "Organ Culture Techniques" by people in Harvard Catalyst Profiles by year, and whether "Organ Culture Techniques" was a major or minor topic of these publication. (
  • Below are the most recent publications written about "Organ Culture Techniques" by people in Profiles. (
  • for suspension culture process uses liquid growth medium and for explant uses solid medium, the medium should contain all basic nutrients in appropriate quantity witch support to the growth of organ or cells under controlled conditions. (
  • whereby 3 (7.5%) of cultures provided positive growth of Salmonella . (
  • The present study compares the effectiveness of two culture techniques for recovery of Salmonella from environmental samples obtained in a large-animal referral veterinary hospital during a Salmonella outbreak. (
  • Salmonella was recovered from 49.7% (73/147) of samples using the TBG-RV-XLT4 technique, but only 10.2% (15/147) of samples using the SEL-XLD method. (
  • Salmonella was much more likely to be recovered using the TBG-RV-XLT4 method, and there appeared to be five times more false-negative results using the SEL-XLD technique. (
  • Environmental contamination with Salmonella may be underestimated by certain culture techniques, which may impair efforts to control spread in veterinary hospitals. (
  • Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. (
  • Forum for prokaryotic and eukaryotic culture, cell culture related assays (e.g. (
  • The bovine pulmonary artery endothelial ( BPAE ) cells presented in the digital image above were resident in an adherent culture stained for F-actin with Alexa Fluor 488 conjugated to phalloidin (green fluorescence), and for DNA with the bis-benzimidazole dye Hoechst 33258 (blue fluorescence). (
  • Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. (
  • A new enhanced technique for urine culture, known as the enhanced quantitative urine culture (EQUC) protocol, detected significantly more pathogens than standard urine culture in women with symptoms of urinary tract infection (UTI). (
  • Comparison of collection techniques for quantitative urine culture in dogs. (
  • Fingerprint Dive into the research topics of 'Comparison of collection techniques for quantitative urine culture in dogs. (
  • The culture of organoids has permitted researchers access to a highly physiologically relevant system for studying human disease. (
  • I am new to research world and would like to learn more about human lymphoblasts culture techniques, and general rules used in tissue culture. (
  • Just like human society that developed a wide array of cultures and technology based on their history and environmental influences, the different orchid genera also have evolved growth mechanisms that allowed them to thrive in their native habitats. (
  • Innovative in vitro exposure techniques that have been developed to provide direct exposure of human lung cells to inhaled materials and nanomaterials will be discussed. (
  • By nature, techniques tend to become general and to multiply across human populations. (
  • Correction to: Effects of culturing technique on human peripheral blood lymphocytes response to proton and X-ray radiation. (
  • Conclusions This study explains and overcomes most of the variability in the success in observing neurogenesis in cultures of adult human olfactory epithelium. (
  • The techniques presented here make the human olfactory epithelium a useful model for clinical research into certain olfactory dysfunctions and a model for the causes of neurodevelopmental and neurodegenerative diseases. (
  • 3D-HMM facilitates the culture of human, non-human primates, and Beagle hepatocytes in spheroid cultures. (
  • Human recombinant hepatocyte growth factor expressed in CHO cells, for in vitro research, including culture media for hepatocytes and animal-component free culture media. (
  • In addition, the culture was immunofluorescently labeled with Alexa Fluor 568 conjugated to goat secondary antibodies that target mouse anti-PDI (protein disulfide isomerase) primary antibodies (red fluorescence). (
  • Popular culture genres of the mid-nineteenth century--the anti-slavery almanac, dime novel, panorama, and propaganda play--provide secondary school teachers with an historical medium to help students understand the conditions of slavery. (
  • We can be also produced to the production of a secondary metabolite by the help of this technique. (
  • George EF, Hall MA, De Klerk G-J (2008b) The component of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems. (
  • Adult neural stem cells are thought of as having a lot of potential for therapeutic applications, but they are fragile and difficult to keep alive in cell culture media (Petri dishes), making them a challenge to study. (
  • Open Culture scours the web for the best educational media. (
  • Open Culture ( and our trusted partners use technology such as cookies on our website to personalise ads, support social media features, and analyze our traffic. (
  • Once outside the animal, remove the swab and introduce it directly into a tube with culture media (e.g. (
  • Standard cell culture media contain 40 to 50 ingredients," said Ince, who was both first author and corresponding author for the article. (
  • Third, cell culturing and analysis in microfluidic devices uses only extremely small volumes of culturing media and analysis buffer. (
  • In this dissertation a non-adherent immortal cell line was studied under static media flow conditions inside a CO[subscript]2 incubator and under dynamic media flow conditions in a novel portable cell culture chamber. (
  • Various specific recipes for algal culture media are described by Vonshak (1986). (
  • The complexity and cost of the above culture media often excludes their use for large-scale culture operations. (
  • We complement our product range of hepatocytes and the HEPAC 2 cell culture system with a variety of cell culture media, including growth factors and collagen products. (
  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. (
  • are capable bf growing in suspension whereas other cell lines requin a solid culture substratum for their growth. (
  • The publicized work of Carrel and his associates at the Rockefeller Institute established the practice of long-term tissue culture for a wide variety of cells. (
  • The Transcendental Meditation technique (or abbreviated as TM) is the technique associated with the practice of Transcendental Meditation developed by Indian spiritual figure Maharishi Mahesh Yogi. (
  • Hence, blood cultures have become critically important and frequently performed test in clinical. (
  • Outpatient blood cultures: progress and unanswered questions. (
  • The article examines the progress and unanswered questions on the outpatient blood cultures. (
  • Mycoplasma contamination of cell culture is a growing concern for most of the research scientists for decades. (
  • This technique could potentially be used with plants that have associated endophytes or other contamination problems to establish callus tissue for research and/or commercial propagation efforts. (
  • Standard urine culture was highly sensitive for detecting E coli but performed poorly for detecting non-E coli pathogens. (
  • The EQUC protocols may detect more pathogens than standard urine culture, but their utility in the clinical setting has not been established. (
  • For the researcher new to cell culture, this handbook provides a wealth of information-from the sourcing of cell lines, laboratory design and safety, to aspects of cryopreservation, quality control and cell line authentication. (
  • There were 176 positive cultures. (
  • The centrifugation technique recovered 73% of the positive cultures. (
  • Blood culture technique based on centrifugation: clinical evaluation. (
  • Basic cell culture techniques for bioscience research and commercial applications. (
  • The European Collection of Authenticated Cell Cultures (ECACC) was established in 1985 as a cell culture collection to service the research community and provide an International Depository Authority recognized patent depository for Europe. (
  • We continue to expand upon this partnership, and now are able to offer an even greater array of cell lines, cell culture products, knowledge and services to the global research community. (
  • Protocols for the use of induced pluripotent stem cells and for growing cells in 3D cell culture , two areas of growing importance to cell culture research, have been added to this latest edition of the handbook. (
  • Many tools and techniques have been developed for organoid culture, however depending on the cell type, research area and experimental goals, it can be difficult to identify the right method. (
  • Some areas of research depend more heavily than others on cell culture techniques. (
  • - The most complete resource on the techniques, equipment,principles, and practices of animal cell culture Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. (
  • Thus Carrel's legacy had a mixed influence on the development of tissue culture techniques, which have been widely used in the fields of embryology and stem cell research. (
  • Carrel's work with tissue culture followed the research of Ross Granville Harrison . (
  • Overall, these laboratories aim to expose students to mammalian cell culture and basic techniques and help them to conceptualize their application in scientific research. (
  • As a member of the European Reference Laboratory Network for Alternatives to Animal Testing we offer modern cell culture techniques and we support you with adequate cell culture protocols for your research. (
  • It is generally believed that the quality of research is improving, with some researchers stating that growing scientific evidence supports the therapeutic value of the technique. (
  • Eighty-seven percent of infants with a positive urine culture result collected by bag technique revealed a mixture of more than three organisms. (
  • However, we are not able to grow some of the key organisms in pure cultures, so we need to use culture independent approaches. (
  • Traditional plant breeding is enhanced with techniques such as embryo culture to rescue recalcitrant hybrids. (
  • Bridgen MP (1994a) A review of plant embryo culture. (
  • In: George EF, Hall MA, De Klerk GJ (eds) Automation and environmental control in plant tissue culture. (
  • do Valle Rego L, Tadeu de Faria R (2001) Tissue culture in ornamental plant breeding: a review. (
  • George EF, Hall MA, De Klerk G-J (2008a) Plant tissue culture procedure - background. (
  • The symposium examined the usefulness for plant breeding purposes of somaclonal variation, i.e. the occurrence of mutations during in vitro culture with or without the application of ionizing radiation or other mutagens. (
  • In vitro culture techniques considerably accelerate the plant breeding cycle. (
  • The technique of efficient & rapid non-test tube plant cloning (TERNPC) is a completely new system for rapid plant propagation, first invented by Professor Li Changxiao. (
  • Akin-Idowu, P.E., Ibitoye, D.O. and Ademoyegun, O.T. (2009) Tissue Culture as a Plant Production Technique for Horticultural Crops. (
  • Although plant tissue culture has been a successful method to propagate many species, V. aestivalis has not yet been reliably grown as pure callus culture due to a fungal endophyte that exists within the plant. (
  • Quantitative Determination of Nitidine from Roots and Plant Tissue Culture Extracts of Toddalia asiatica (Linn. (
  • Tissue culturing is the popular process of creating clones from a source plant. (
  • Can mammalian and plant cell culture be done in same hood? (
  • It is an important technique for maintaining a'pm or piece of animal or plant tissue alive after their culture dishes. (
  • Plant tissue culture is a major technique or method to the culture of a plant cell, organs, in in-vitro under controlled conditions with a suitable culture medium. (
  • plant tissue culture is a very suitable technique to cultivate a new plant by plant parts with very least of time. (
  • basic of plant tissue culture is cell totipotency power. (
  • We van large scale culture of plant cells to obtain biochemicals in bio-reactors. (