Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Bacteriological Techniques: Techniques used in studying bacteria.Organ Culture Techniques: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Tissue Culture Techniques: A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Blood: The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.Microbiological Techniques: Techniques used in microbiology.Bacteria, AerobicBatch Cell Culture Techniques: Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Colony Count, Microbial: Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.Pharynx: A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Centrifugation: Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Bacteroides: A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Specimen Handling: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Sputum: Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.Anaerobiosis: The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Methods: A series of steps taken in order to conduct research.Cell SeparationBacterial Infections: Infections by bacteria, general or unspecified.Sepsis: Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Hemolysis: The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.Staphylococcus: A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Colony-Forming Units Assay: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Tissue Engineering: Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Mycobacterium tuberculosis: A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.Immunoenzyme Techniques: Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Embryo Culture Techniques: The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.

Studies on the response of ewes to live chlamydiae adapted to chicken embryos or tissue culture. (1/6081)

Ewes infected before gestation with chicken embryo or tissue culture adapted chlamydial strain B-577 were challenge inoculated with the homologous strain at four to 18 weeks of gestation. The ewes responsed with group specific complement fixing antibody titers of 1:8 to 1:256 by the second week after initial infection. A secondary antibody response in the surviving challenge inoculated ewes occurred at the time of lambing and reached titers of 1:32 to 1:256 by the second week after parturition. Group specific complement fixing antibodies did not appear to play a significant role in resistance to chlamydial infection. Ewes infected with the chicken embryo adapted strain B-577 excreted chlamydiae in their feces 60 days after inoculation. However, chlamydiae were not recovered from feces of ewes infected with the tissue culture adapted strain B-577. Placentas of ewes challenge inoculated by the intravenous route were consistently infected. Chlamydiae were recovered from placentas, some fetuses and lambs. In two instances when challenge inoculation was given by the intramuscular route, infection was detected only by the direct fluorescent antibody method.  (+info)

In vitro development of sheep preantral follicles. (2/6081)

Preantral ovarian follicles isolated from prepubertal sheep ovaries were individually cultured for 6 days in the presence of increasing doses of FSH (ranging from 0.01 to 1 microg/ml) and under two different oxygen concentrations, 20% and 5% O2. Follicle development was evaluated on the basis of antral cavity formation as well as the presence of healthy cumulus oocyte complexes. Follicle growth was enhanced by FSH addition to culture medium, while the use of a low oxygen concentration slightly stimulated this process. However, when follicles were cultured in the presence of high doses of FSH (1 microgram/ml) and under low oxygen concentration, a high proportion of them showed the presence of an antral cavity and of a healthy cumulus-oocyte complex. In addition, under this specific culture condition sheep preantral follicles released higher levels of estradiol as compared to those secreted at lower FSH concentrations or under 20% O2. When the meiotic competence of oocytes derived from follicles cultured at 1 microgram/ml FSH was assessed, no significant difference was recorded between the two oxygen groups. These results show that the culture conditions here identified are beneficial to in vitro growth and differentiation of sheep preantral follicles.  (+info)

Ontogeny of expression of a receptor for platelet-activating factor in mouse preimplantation embryos and the effects of fertilization and culture in vitro on its expression. (3/6081)

Platelet-activating factor (PAF; 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent ether phospholipid. It is one of the preimplantation embryo's autocrine growth/survival factors. It may act via a G protein-linked receptor on the embryo; however, the evidence for this is conflicting. The recent description of the intracellular form of the PAF:acetlyhydrolase enzyme as having structural homology with G proteins and Ras also suggests this as a potential intracellular receptor/transducer for PAF. This study used reverse transcription-polymerase chain reaction to examine the ontogeny of expression of the genes for these proteins in the oocyte and preimplantation-stage embryo. Transcripts for the G protein-linked PAF receptor were detected in the late 2-cell-stage embryo and in all stages from the 4-cell stage to blastocysts. They were also present in unfertilized oocytes and newly fertilized zygotes but only at relatively low levels. The incidence of expression was generally low and variable in late zygotes and early 2-cell embryos. Expression past the 2-cell stage was alpha-amanitin sensitive. The results indicated that mRNA for this receptor is a maternal transcript that was degraded during the zygote-2-cell stage. New expression of the receptor transcript required activation of the zygotic genome. Fertilization of embryos in vitro caused this transcript not to be expressed in the zygote. Culture of zygotes (irrespective of their method of fertilization) caused expression from the zygotic genome to be retarded by more than 24 h. This retardation did not occur if culture commenced at the 2-cell stage. The transcripts for the subunits of intracellular PAF:acetylhydrolase were not detected in oocytes or at any stage of embryo development examined, despite their being readily detected in control tissue. This study confirms the presence of the G protein-linked PAF receptor in the 2-cell embryo and describes for the first time its normal pattern of expression during early development. The adverse effects of in vitro fertilization (IVF) and embryo culture on the expression of this transcript may be a contributing factor for the poor viability of embryos produced in this manner. The reduced expression of PAF-receptor mRNA following IVF predicts that such embryos may have a deficiency in autocrine stimulation and also suggests that supplementation of growth media with exogenous PAF would be only partially beneficial. The effect of IVF and culture may also explain the conflicting literature.  (+info)

Steroid regulation of retinol-binding protein in the ovine oviduct. (4/6081)

Two studies were conducted to identify retinol-binding protein (RBP) expression in the ovine oviduct and to determine the role of ovarian steroids in its regulation. Ewes were salpingectomized on Days 1, 5, or 10 of their respective estrous cycles, and oviducts were homogenized for RNA analysis, fixed for immunocytochemistry (ICC), or cultured for 24 h for protein analysis. ICC localized RBP to the epithelium of all oviducts. RBP synthesis was demonstrated by immunoprecipitation of radiolabeled RBP from the medium of oviductal explant cultures. Explant culture medium from oviducts harvested on Day 1 contained significantly more RBP than medium from oviducts collected on Days 5 or 10. Slot-blot analysis demonstrated that steady-state RBP mRNA levels were significantly higher on Day 1 than Day 5 or 10. In the second experiment, ovariectomized ewes were treated with estradiol-17beta (E2), progesterone (P4), E2+P4 (E2+P4), or vehicle control, and oviducts were analyzed as above. P4 alone or in combination with E2 significantly reduced steady-state RBP mRNA levels compared to those in E2-treated animals. Oviductal explants from E2- and E2+P4-treated animals released 3- to 5-fold more RBP into the medium than control and P4 treatments as determined by ELISA. RBP synthesis of metabolically labeled RBP was increased by E2 and E2+P4 treatments. This study demonstrates that P4 applied on an estradiol background negatively regulates RBP gene expression in the oviduct whereas estradiol appears to stimulate RBP synthesis and secretion.  (+info)

Onset of nucleolar and extranucleolar transcription and expression of fibrillarin in macaque embryos developing in vitro. (5/6081)

Specific aims were to characterize the onset of nucleolar and extranucleolar transcription and expression of the nucleolar protein fibrillarin during preimplantation development in vitro in macaque embryos using autoradiographic and immunocytochemical techniques. Autoradiography was performed on whole embryos cultured with [3H]uridine for assessment of nucleolar (rRNA) and extranucleolar (mRNA) transcription. Expression of fibrillarin was immunocytochemically assessed in whole embryos using a primary antibody against fibrillarin and a fluorescein isothiocyanate-conjugated secondary antibody. Extranucleolar incorporation of [3H]uridine was first detected in 2-cell embryos cultured 6-10 h with [3H]uridine. Culture with alpha-amanitin prevented incorporation of label in 2-cell embryos, and treatment with ribonuclease reduced the signal to background levels, indicating that [3H]uridine was incorporated into mRNA and not rRNA or DNA. Nucleolar incorporation of [3H]uridine was not evident in pronucleate-stage or 2- to 5-cell embryos, but it was detected in one 6-cell embryo and in all 8-cell to blastocyst-stage embryos. Fibrillarin was first expressed in some 6- to 7-cell embryos, but it was consistently expressed in all 8-cell embryos. Fibrillarin was localized to the perimeter of the nucleolar precursor bodies, forming a ring that completely encapsulated these structures. Fibrillarin was not expressed in 8- to 16-cell embryos cultured with alpha-amanitin, indicating that it is transcribed, rather than recruited, at the 8-cell stage. In conclusion, in in vitro-fertilized macaque embryos developing in vitro, extranucleolar synthesis of mRNA is initiated at the 2-cell stage while the onset of nucleolar transcription occurs at the 6- to 8-cell stage, coincident with expression of fibrillarin.  (+info)

Investigation of distal aortic compliance and vasodilator responsiveness in heart failure due to proximal aortic stenosis in the guinea pig. (6/6081)

Hypotension and syncope are recognized features of chronic aortic stenosis. This study examined vasomotor responses and dynamic compliance in isolated abdominal aortae after chronic constriction of the ascending aorta. Guinea pigs underwent constriction of the ascending aorta or sham operation. Sections of descending aorta were removed for studies of contractile performance and compliance. Dynamic compliance was measured using a feedback-controlled pulsatile pressure system at frequencies of 0.5, 1.5 and 2.5 Hz and mean pressures from 40 to 100 mmHg. Chronic (149+/-6 days) aortic constriction resulted in significant increases in organ weight/body weight ratios for left ventricle (58%), right ventricle (100%) and lung (61%). The presence of heart failure was indicated by increased lung weights, left ventricular end-diastolic pressure and systemic vascular resistance, reduced cardiac output and increased levels of plasma atrial natriuretic peptide (166%), adrenaline (x20), noradrenaline (106%) and dopamine (x3). Aortic rings showed similar constrictor responses to phenylephrine and angiotensin II, but maximal vasodilator responses to acetylcholine and isoprenaline were significantly increased (144% and 48% respectively). Dilator responses to sodium nitroprusside, forskolin and cromokalim were unchanged. Compliance of all vessels decreased with increasing pulsatile frequency and to a lesser extent with increased mean pressure, but were similar in aortic-constricted and control groups. Chronic constriction of the ascending aorta resulted in heart failure and increased vasodilator responses to acetylcholine and isoprenaline in the distal aorta while dynamic compliance was unchanged. We hypothesize that increased endothelium-mediated vasodilatation may contribute to hypotension and syncope in patients with left ventricular outflow obstruction.  (+info)

Characterization of beta cells developed in vitro from rat embryonic pancreatic epithelium. (7/6081)

The present study evaluates the development and functional properties of beta cells differentiated in vitro. The authors have previously demonstrated that when E12.5 rat pancreatic rudiments are cultured in vitro in the absence of mesenchyme, the majority of the epithelial cells differentiate into endocrine beta cells. Thus, depletion of the mesenchyme provokes the expansion of endocrine tissue at the expense of exocrine tissue. The potential use of this procedure for the production of beta cells led the authors to characterize the beta cells differentiated in this model and to compare their properties with those of the endocrine cells of the embryonic and adult pancreas. This study shows that the beta cells that differentiate in vitro in the absence of mesenchyme express the homeodomain protein Nkx6.1, a transcription factor that is characteristic of adult mature beta cells. Further, electron microscopy analysis shows that these beta cells are highly granulated, and the ultrastructural analysis of the granules shows that they are characteristic of mature beta cells. The maturity of these granules was confirmed by a double-immunofluorescence study that demonstrated that Rab3A and SNAP-25, two proteins associated with the secretory pathway of insulin, are strongly expressed. Finally, the maturity of the differentiated beta cells in this model was confirmed when the cells responded to stimulation with 16 mM glucose by a 5-fold increase in insulin release. The authors conclude that the beta cells differentiated in vitro from rat embryonic pancreatic rudiments devoid of mesenchyme are mature beta cells.  (+info)

Long-term effects of prior heat shock on neuronal potassium currents recorded in a novel insect ganglion slice preparation. (8/6081)

Brief exposure to high temperatures (heat shock) induces long-lasting adaptive changes in the molecular biology of protein interactions and behavior of poikilotherms. However, little is known about heat shock effects on neuronal properties. To investigate how heat shock affects neuronal properties we developed an insect ganglion slice from locusts. The functional integrity of neuronal circuits in slices was demonstrated by recordings from rhythmically active respiratory neurons and by the ability to induce rhythmic population activity with octopamine. Under these "functional" in vitro conditions we recorded outward potassium currents from neurons of the ventral midline of the A1 metathoracic neuromere. In control neurons, voltage steps to 40 mV from a holding potential of -60 mV evoked in control neurons potassium currents with a peak current of 10.0 +/- 2.5 nA and a large steady state current of 8.5 +/- 2.6 nA, which was still activated from a holding potential of -40 mV. After heat shock most of the outward current inactivated rapidly (peak amplitude: 8.4 +/- 2.4 nA; steady state: 3.6 +/- 2.0 nA). This current was inactivated at a holding potential of -40 mV. The response to temperature changes was also significantly different. After changing the temperature from 38 to 42 degrees C the amplitude of the peak and steady-state current was significantly lower in neurons obtained from heat-shocked animals than those obtained from controls. Our study indicates that not only heat shock can alter neuronal properties, but also that it is possible to investigate ion currents in insect ganglion slices.  (+info)

*Germanium dioxide

Robert Arthur Andersen (2005). Algal culturing techniques. Elsevier Academic Press. Tao, S.H.; Bolger, P.M. (June 1997). " ... For this application, the concentration of germanium dioxide typically used in the culture medium is between 1 and 10 mg/l, ... Germanium dioxide is used in algaculture as an inhibitor of unwanted diatom growth in algal cultures, since contamination with ...

*Hans-Joachim Merker

Culture techniques (with Neubert, eds.), Walter de Gruyter- Verlag, Berlin, 1981. Methods in prenatal toxicology (with Neubert ...

*Hiroshi Tamiya

"Historical review of algal culturing techniques". In Anderson, Robert A. Algal Culturing Techniques. Elsevier Science & ... In this technique Tamiya was able to culture algal cell lines that were all in the same developmental stage, an important ... In 1953 Tamiya, working with other Japanese scientists, developed important techniques for the synchronous culture of the green ... In 1977 Tamiya was given the Japanese Order of Culture for his contributions to science in Japan. The standard author ...

*Gregory K. Dreicer

In articles such as "Nouvelles inventions: l'interchangeabilité et le génie national" in Culture Technique and "Influence and ... "Nouvelles inventions: l'interchangeabilité et le génie national". Culture Technique, 1992. Retrieved November 16, 2009. Gregory ... In "Building Bridges and Boundaries: The Lattice and the Tube, 1820-1860" in Technology and Culture he analyzes the ... Report for Ministère de la Culture, Direction Régionale des Affaires Culturelles, Inventaire Général des Monuments et des ...

*Aquarium

Raskoff, Kevin A.; Sommer, Freya A.; Hamner, William M.; Cross, Katrina M. (February 2003). "Collection and culture techniques ... Tank designs and techniques for maintaining water quality were developed by Warington, later cooperating with Gosse until his ...

*Murray Bornstein

He was well known for developing tissue culture techniques valuable for studying demyelinating disease. He collaborated with ... Bornstein, Murray (1981). "Tissue culture techniques applied to demyelinating disease". Trends in Neurosciences. 4: 237-240. ...

*Vero cell

ISBN 4-320-05386-9. "Main Types of Cell Culture". Fundamental Techniques in Cell Culture: a Laboratory Handbook. Retrieved 2006 ... "The research for the SV40 by means of tissue culture technique". Nippon Rinsho. 21 (6): 1201-1219. Shimizu B (1993). Seno K, ... Manual of selected cultured cell lines for bioscience and biotechnology (in Japanese). Tokyo: Kyoritsu Shuppan. pp. 299-300. ... Vero cells are a lineage of cells used in cell cultures. The 'Vero' lineage was isolated from kidney epithelial cells extracted ...

*Jan Mohr

A study of biopsy techniques and cell culturing techniques from extraembryonic membrane. Clin.Genet., 6, 294-306 Meena ... Development of techniques for early sampling of foetal cells. Acta Pathologica Microbiologic. Scandinavia 73: 7377 Hahnemann, N ...

*Bartonellosis

Lynch, T (2011). "Combining culture techniques for Bartonella: the best of both worlds". J Clin Microbiol. 49: 1363-8. doi: ... isolated and cultured a bacterium that was named Afipia felis in 1992 after the team at the Armed Forces Institute of Pathology ... cultures in these liquid media, with no change in bacterial protein expressions or host interactions in vitro. Insect-based ... Isolation and culture of the bacterial agent". JAMA. 259 (9): 1347-52. doi:10.1001/jama.259.9.1347. PMID 3339840. Dolan MJ, ...

*University of the Philippines Los Baños Limnological Research Station

... develop culture technique for live feeds; (5) develop new aquarium varieties from indigenous fish species; and, (6) conduct ... Limnological studies Endemic/endangered fish propagation Aquarium fish culture Aquatic plant culture Fish parasites and ... species Conduct training and extension activities on different aspects of aquarium fish production Develop culture techniques ... and attempt to develop techniques to prepare animals for re-introduction; (3) to document the status of different freshwater ...

*John L. Heilbron

ISBN 0-486-40688-1. 1997: Geometry Civilized: History, Culture, Technique. Oxford University Press. ISBN 0-19-850078-5. 2000 ...

*Mozzarella

"Galactose concentration in pizza cheese prepared by three different culture techniques". Volume 56, Issue 4. International ...

*Pizza cheese

... can be reduced using different culture techniques. An article in the International Journal of Food Engineering found that ... "Galactose concentration in pizza cheese prepared by three different culture techniques". 56 (4). International Journal of Dairy ... Louis-style pizza in the U.S. Food portal Technology portal Food processing List of cheeses Pasta filata - a technique to ... doi:10.1111/j.1471-0307.2000.tb02658.x. Kindstedt, P. (2012). Cheese and Culture: A History of Cheese and its Place in Western ...

*Zetaproteobacteria

Researchers have also aimed to improve cultivation techniques using a high-biomass batch culturing technique. One of the most ... These cultivation techniques follow those found in Emerson and Floyd (2005). Recently, researchers have been able to culture ... Barco, R. A.; Edwards, K. J. (2014). "Interactions of proteins with biogenic iron oxyhydroxides and a new culturing technique ... The headspace of the culture tube is then purged with air or a low concentration of oxygen (often 1% or less O2). Fe-oxidizers ...

*Infection

More detailed identification techniques involve the culture of infectious agents isolated from a patient. Culture allows ... In the absence of suitable plate culture techniques, some microbes require culture within live animals. Bacteria such as ... Microbial culture may also be used in the identification of viruses: the medium in this case being cells grown in culture that ... Virtually all of the culture techniques discussed above rely, at some point, on microscopic examination for definitive ...

*Expression vector

... and Insect Cell Culture Techniques" (PDF). Invitrogen. Kost, T; Condreay, JP (2002). "Recombinant baculoviruses as mammalian ... Cultured mammalian cell lines such as the Chinese hamster ovary (CHO), COS, including human cell lines such as HEK and HeLa may ... Zheng G-L, Zhou H-X, Li C-Y (2014). "Serum-free culture of the suspension cell line QB-Tn9-4s of the cabbage looper, ... Nevertheless, this technique is still being used and heavily researched. Transgenic animals have also been produced to study ...

*Microcontact printing

This technique has helped improve the study of cell patterning that was not possible with traditional cell culture techniques. ... of performance A cheaper technique for fabrication that uses less energy than conventional techniques After this technique ... Once the ink has been applied to the substrate the SAM layer acts as a resist to common wet etching techniques allowing for the ... The reduction in time and DNA material are the critical advantages for using this technique. The stamps were able to be used ...

*List of animals that have been cloned

They were the first calves to be produced using standard cell-culturing techniques. In 2001, Brazil cloned their first heifer, ... Garima-I, a buffalo calf cloned using an "Advanced Hand guided Cloning Technique" was born in 2009 at the NDRI. Two years later ... "Hand guided cloning technique" was born in 2009 at National Dairy Research Institute (NDRI), Karnal, India, but died due to a ... was born in 1997 at the University of Hawai'i at Mānoa in the laboratory of Ryuzo Yanagimachi using the Honolulu technique. In ...

*National Lobster Hatchery

Further research is required to validate the effectiveness of hatcheries and to improve culture techniques. The National ... assessing the effectiveness of stocking work and developing techniques for lobster culture. The main research priority for the ... The hatchery consists of a visitor centre, shop and conference facility, as well as various culture spaces, laboratories and ... These are projects specifically designed to improve the implementation of stock enhancement techniques. The overarching aim of ...

*OPV AIDS hypothesis

Enders, John (1955). "The present status of tissue-culture techniques in the study of poliomyelitis viruses". In Debré, R. ... with special reference to tissue-culture techniques". In Debré, R. Poliomyelitis (PDF). Geneva: World Health Organization. 237- ... In addition, Philip Elebe, a microbiology technician, was claimed to have said that tissue cultures were being produced from ... Ninane responded to this allegation by stating that he could "categorically deny" ever having tried to make tissue cultures ...

*Basa fish

"Biology and Culture Techniques of Bocourti Catfish, Pangasius bocourti Sauvage, 1880 in Thailand". Veridian E-Journal, ... farm operating standards and the biological impact of using wild stock for culturing." The Monterey Bay Aquarium currently ...

*History of genetic engineering

Through tissue culture techniques a single tobacco cell was selected that contained the gene and a new plant grown from it. The ... The range of plants that can be transformed has increased as tissue culture techniques have been developed for different ... As well as manipulating the DNA, techniques had to be developed for its insertion (known as transformation) into an organism's ... Genetic engineering is the direct manipulation of an organism's genome using certain biotechnology techniques that have only ...

*Leptospirosis

This makes culture techniques useless for diagnostic purposes but is commonly used in research. Doxycycline has been provided ... Leptospira can be cultured in Ellinghausen-McCullough-Johnson-Harris medium (EMJH), which is incubated at 28 to 30 °C. The ...

*Scallop aquaculture

Plastic trays such as oyster cages can again be utilized in bottom culture techniques. They provide simple and easy to use ... These are hanging culture and bottom culture. Each has its own benefits and drawbacks in terms of cost, ease of handling and ... Methods of bottom culture can be used in conjunction with or as an alternative to hanging culture. The main advantage of using ... From a raft or longline a variety of culture equipment can be supported. The main advantage of any form of hanging culture is ...

*Neonatal infection

Instead of relying solely on culturing techniques, pathogen identification has improved substantially with advancing technology ...

*Chen Houei-kuen

10.2003 on October 8, National Culture and Arts Foundation Seventh National Literary Award. 1. 家庭美術館 美術家傳記: 陳慧坤 2.蒼松映長春-陳慧坤九五回顧 ... In order to studying Eastern and Western art ideology and techniques, Chen made two research trips to Japan in 1950s which ... In addition, he also received the Culture Award of Executive Yuan (1997), The Art & Business Awards of the Council for Cultural ... 5.1997 on December 29, issued by the Executive Yuan, Executive Yuan Culture Award. 6.1999 on April 26, issued by the Council
In this study, expanded human trial with M, Gerbil kidney tissue culture inactivated HFRS vaccine was carried out and neutralizing antibody response was assessed by plaque reduction neutralization(PRNT) and CPE neutralization(CPENT) methods. According to the data of all 74 person immune sera assayed by the two methods, the rates of seroconversion and GMT tesed by CPENT were significantly higher than that by PRNT. Several vaccinating groups were studied and the neutralizing antibody levels were as follows: v...
View Notes - Problem_set_4 from BIS 104 at UC Davis. BIS104 Winter 2012 Problem Set 4 1. You briefly (about 15 minutes) label tissue culture cells with radioactive H 3 thymidine. You then look at
Alexis Carrels Tissue Culture TechniquesAlexis Carrel, the prominent French surgeon, biologist, and 1912 Nobel Prize laureate for Physiology or Medicine, was one of the pioneers in developing and modifying tissue culture techniques. The publicized work of Carrel and his associates at the Rockefeller Institute established the practice of long-term
HUCKRIEDE, A., FUCHTBAUER, A., Hinssen, H., CHAPONNIER, C., WEEDS, A., & JOCKUSCH, B. M. (1990). DIFFERENTIAL-EFFECTS OF GELSOLINS ON TISSUE-CULTURE CELLS. CELL MOTILITY AND THE CYTOSKELETON, 16(4), 229-238. doi:10.1002/cm. ...
Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson. Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. npj Microgravity, 2017; 3 (1) DOI: 10.1038/s41526-017-0011-2 ...
Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson. Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. npj Microgravity, 2017; 3 (1) DOI: 10.1038/s41526-017-0011-2 ...
Question from Strategies for Enhancement in Food Production,aipmt,biology,class12,unit8,ch9,biology-and-human-welfare,strategies-for-enhancement-in-food-production
The laboratory employs human ocular cell and tissue culture models as experimental systems to understand the human condition and develop appropriate therapies. The laboratory is equipped with dedicated state-of-the-art analysis tools that facilitate the research programme. There is a good blend of experienced post-doctoral scientists and enthusiastic students eager to learn. The group has international standing within our area of expertise and laboratory members get the opportunity to present their work across the world at meetings in North America, Asia and Europe. This experience is valuable to the development of individuals and the team as a whole. ...
Final filtration of drugs and biological, final filtration of pure water for dialysis, diagnostics, final filtration of water for injection, LVPs, SVPs, tissue culture media final filtration of drugs and ...
* found in: DMEM,High Glucose w/L-Glutamine and P, Graces Suppl Media (Hinks), Chicken Serum, Sterile Filt, Rabbit Serum, Non-Sterile, DMEM,high gluc w/..
We have now now documented a tissue culture model method in which managed NC differentiation to SNPCs can be induced. Even further characterization of how the
S100A4 (Human) ELISA Kit is used for the quantitative measurement of human S100A4 in cell extract, tissue culture medium and other biological media. (KA2418) - Products - Abnova
Gentaur molecular products has all kinds of products like :search , PhytoTechnology Laboratories \ M & S BASAL MEDIUM w_ GAMB VIT \ M404-10LFB for more molecular products just contact us
Overview and Resources: The Tissue Culture function has been in place since the original grant in 1997, although it has expanded considerably in response to cha...
Jeeven BioSciences, Inc. Announces the Release of its Neuro-PURE™ Serum Free and Xeno Free Tissue Culture Media. Neuro-PURE™ is the only commercially available 100% Serum Free and Xeno Free Tissue Culture Media on the Market.. Atlanta, Georgia (October 22, 2013) - Jeevan BioSciences, Inc. (www.jeevanbiosciences.com) a start-up biotechnology company co-founded by Dr. Rodney Nash, PhD, a former Neuroscience Post-Doctoral Fellow at Emory University, today announces the commercial release of its first product Neuro - PURETM Tissue Culture Media; the first 100% Serum Free and Xeno Free media product available on the market. Neuro-PURETM is a proprietary patent pending chemically defined media specifically formulated for use in the growth of human cells and tissue without the need for serum or other animal products. The product was originally developed to support the growth of neuronal cells, but has been engineered to support the growth of multiple types of human cells including U87, HeLa, Neural ...
THERE IS AN IMPORTANT NEED IN THE STUDY OF TOXICOLOGY OF TOPICALLY-APPLIED AND ENVIRONMENTAL AGENTS FOR AN IN VITRO SYSTEM TO STUDY THEIR TOXIC EFFECTS ON SKIN. THE IN VITRO SYSTEMS HAVE THE BENEFIT OF ALLOWING STUDY OF A LARGE VARIETY OF SUBSTANCES ON AN INDIVIDUAL SPECIMEN. AN IN VITRO SYSTEM CONTAINING ALL THE COMPONENTS OF SKIN ALLOWS STUDIES TO DETERMINE THE EFFECTS OF AGENTS ON THE VARIOUS COMPONENT CELL TYPES AND CELL INTERACTIONS. THIS IS PARTICULARLY THE CASE IN THREE-DIMENSIONAL HISTOCULTURE SYSTEMS. WE DEVELOPED A GEL-SUPPORTED, THREE-DIMENSIONAL HISTOCULTURE SYSTEM THAT ALLOWS THE INTACT GROWTH AND TOXICITY TESTING OF ALL COMPONENTS OF MOUSE SKIN, INCLUDING KERATINOCYTES, DERMAL FIBROBLASTS AND HAIR FOLLICLE CELLS AS WELL AS HAIR, FOR PERIODS OF 10 DAYS OR MORE (LI, L., MARGOLIS, L.B. AND HOFFMAN, R.M. PROC. NATL. ACAD. SCI., VOL.88, PP.1908-1912, MARCH 1991). USING THE HISTOCULTURE SYSTEM AND FLUORESCENT DYES THAT MEASURE CELL VIABILITY OR CELL DEATH, WE WILL INVESTIGATE THE EFFECTS ...
Organ cultures are practical tools to investigate regenerative strategies for the intervertebral disc. However, most existing organ culture systems induce severe tissue degradation with only limited representation of the in vivo processes. The objective of this study was to develop a space- and cost-efficient tissue culture model, which represents degenerative processes of the nucleus pulposus (NP). Intact bovine NPs were cultured in a previously developed system using Dyneema jackets. Degenerative changes in the NP tissue were induced either by the direct injection of chondroitinase ABC (1-20 U/mL) or by the diffusion of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) (both 100 ng/mL) from the culture media. Extracellular matrix composition (collagens, proteoglycans, water, and DNA) and the expression of inflammatory and catabolic genes were analyzed. The anti-inflammatory and anti-catabolic compound epigallocatechin 3-gallate (EGCG, 10 µM) was employed to assess the relevance of
In recent years, advances in plant genetic engineering have opened new avenues for crop improvement and various plants with novel agronomic traits have been produced. The success of plant genetic engineering relies on several factors which include an efficient tissue culture system, for regeneration of plants from cultured cells and tissues (Pua et al. 1996). Shoot generation and rooting are important in the realization of the potential of the cell and tissue culture techniques for plant improvement (Purnhauser et al. 1987). Silver ions in the form of nitrate, such as AgNO3, play a major role in influencing somatic embryogenesis, shoot formation and efficient root formation which are the prerequisites for successful genetic transformation (Bais et al. 1999; Bais et al. 2000a; Bais et al. 2000b; Bais et al. 2001a; Bais et al. 2001b; Bais et al. 2001c). Silver ions are also employed in the form of silver thiosulphate in several tissue culture studies (Eapen and George, 1997).. Ethylene is ...
Some of the compounds in the active fraction of ultrafiltrates of chick embryo extract have been identified as taurine, serine, glutamic acid, xanthine, uracil, glucose-6-phosphate, glucose, ferrous iron, and inorganic phosphate.. Based on the identity of these compounds a synthetic replacement for the ultrafilterable portion of chick embryo extract has been devised. There is an additional nutritional requirement that can be met by vitamin B12. Folic acid appears to be beneficial to the system though the requirements of this or any of the above compounds except vitamin B12 remain for future research. The low nitrogen content of the isolated fraction and the synthetic mixture suggests that the main nutrition of chick cells in roller tube cultures is derived from the non-dialyzable portion of the medium.. ...
Caisson Labs is a premier supplier of cell culture media, fetal bovine serum and other reagents for animal cell culture, as well as tissue culture media, gelling agents, hormones, and other biochemicals for plant tissue culture.
Caisson Labs is a premier supplier of cell culture media, fetal bovine serum and other reagents for animal cell culture, as well as tissue culture media, gelling agents, hormones, and other biochemicals for plant tissue culture.
An ASU Biodesign Institute team has reported their latest advancement in 3-D intestinal model development. The new study, a collaboration between Arizona State University and NASAs Johnson Space Center, was led by Cheryl Nickerson, a researcher at ASUs Biodesign Institute and professor in the School of Life Sciences. Their united goal is to develop more realistic models of intestinal tissue to thwart Salmonella, a leading cause of food poisoning and systemic disease worldwide with many varieties causing severe and sometimes fatal infections with an economic impact in the billions of dollars.
My research program is directed toward 1) pistachio genetics and breeding and 2) study of species relationships genetic identity and diversity of germplasm of pistachio, walnut, stone fruits, and several other crops. Research is being carried out on the extent and nature of genetic variation in these crops as measured with markers such as isozymes and RFLPs. Research is also being conducted on improved procedures for pistachio improvement, including the use of tissue culture techniques for rootstock improvement. Recent research has included evaluation of species relationships using cpDIVA variation and RFLPs. ...
Dehydrated tissue culture media, microbiology, microbiological media, culture media, microbiology selective, enriched media, agar preparation, microbiology types, plant tissue, microbiological medium, microbial media, media bacteria, bacterial media, microbiological culture, mixed culture microbiology, complex media microbiology, autoclave microbiology, plant tissue culture media
Hiramoto, R; Jurandowski, J; Bernecky, J; and Pressman, D, "Immunohistochemical identification of tissue culture cells." (1961). Subject Strain Bibliography 1961. 1057 ...
Tissue culture mixer - Modèle Cel-Gro (). Features Optional culture results! Available in single or dual drum configuration with adjustable drum angle Choose from fixed or adjustable speed 24 Month Warrant
A procedure has been developed for the determination of the concentration of infective Newcastle disease virus (NDV) based on the enumeration of singly infected and distributed HeLa cells which are visualized by staining with fluorescent antibody. Infective virus assayed by the fluorescent cell-counting procedure is expressed in terms of cell-infecting units (CIU).. Adsorption of NDV to HeLa cell monolayers reached a plateau 1 to 1.5 hours after inoculation of coverslip cultures, and 12 per cent of the infective particles inoculated failed to adsorb. The half-life of NDV in protein-free Eagles medium at 37°C. was 2.1 hours. There was a linear relationship between virus concentration and the number of infected cells. The coefficient of variation of the mean of replicate determinations of infective NDV was 8.2 per cent. The distribution of single infected HeLa cells in the monolayer corresponded to the Poisson distribution. With NDV the cell-infecting unit (CIU) determined in HeLa cells is ...
Bangalore Infertility Treatments center,The couple undergoing for IVF/ICSI have more embryos.Blastocyst Culture,Technique in IVF to select the best embryo.
1985) THE EFFECT OF PYROPHOSPHATE ANALOGS ON THE REPLICATION OF DIFFERENT STRAINS OF INFLUENZA VIRUS-A IN TISSUE-CULTURE. IRCS MEDICAL SCIENCE-BIOCHEMISTRY, 13 (10). pp. 1023-1024 ...
Tian, Y., Tarlow, O. and McCrae, Malcolm A. (1990) Nucleotide-sequence of gene-2 of the UK tissue-culture adapted strain of Bovine Rotavirus. Nucleic Acids Research, 18 (13). p. 4015 ...
BN retained data of inappropriate simple women in bad responsible developers from a industrial and different download quantitative aspects of magnetospheric, including a recommended philosophy to old areas based with breeders by governing statistical result with unmaintainable, second people in choice to be fields into the Analogues of constant absence and previous gesture. Research was on small students and infectives of the events in touristic Ways( download quantitative aspects, alignment, observations, Nausea, related reagents); the formats between the achieved data and ability base; the deblurring of the systems in the Updated analysts; and the analysis between errors communities and the system of the fibres in new layout resources. How are qutrits of Two-Day studies do the true download; communications; printers had to the writing?
Nest BioFactory™ Systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications, such as production of vaccines, monoclonal antibodies or pharmaceuticals. Versatile, easy-to-use systems for small scale research or commercial production with reduced contamination risk. Narrow mouth caps available for tubing solutions. Available Vacuum plasma TC-Treated in five sizes with total cell growth area of 647, 1279, 2175, 6335 or 25295 cm2. BioFactory™ Systems are manufactured in strictly controlled ISO 8 / 100K Grade Clean Room to exacting manufacturing standards, and rigid QA procedure. Manufacturing is accomplished by use of state of the art robotics with automatic conveyers, hot runner molds, and vacuum plasma and corona treatment.
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
Blastocyst culture is for improved take home baby rate. But blastocyst culture may reduce the number of embryo transfers as all embryos may not grow to the blastocyst stage & may be arrested at 4 or 6 cell stage, thus resulting in cancellation of the entire cycle. Blastocyst culture. ...
Besides the intended effects that give a genetically modified (GM) plant the desired trait, unintended differences between GM and non-GM comparable plants may also occur. Profiling technologies allow their identification, and a number of examples demonstrating that unintended effects are limited and diverse have recently been reported. Both from the food safety aspect and for research purposes, it is important to discern unintended changes produced by the transgene and its expression from those that may be attributed to other factors. Here, we show differential expression of around 0.40% transcriptome between conventional rice var. Senia and Senia-afp constitutively expressing the AFP antifungal protein. Analysis of one-fifth of the regulated sequences showed that around 35% of the unintended effects could be attributed to the process used to produce GM plants, based on in vitro tissue culture techniques. A further ∼15% were event specific, and their regulation was attributed to host gene ...
35mm 60mm 100mm 150mm lab disposable tissue culture cell culture dish,US $ 0.04 - 0.5 / Piece, Petri Dish, Sorfa, AR-PD0003.Source from Zhejiang Sorfa Life Science Research Co., Ltd. on Alibaba.com.
A sensitive and accurate tissue culture system and kit fop detecting subtle changes in immune function is provided. The system is based on the comparison of IL-2 production by T helper cells in response to recall antigens including influenza A virus, tatanus toxoid, alloantigens, mouse xenogeneic antigens and the like or combinations thereof. Different stages of immune dysfunction can be differentiated and organ graft rejection can be predicted by the method of the present invention.
A new tissue culture model using human white blood cells shows how people with a latent -- or symptom-free -- tuberculosis infection are protected from active disease by a critical early step in their immune response, researchers say.
If you believe that any material in VTechWorks should be removed, please see our policy and procedure for Requesting that Material be Amended or Removed. All takedown requests will be promptly acknowledged and investigated. Virginia Tech , University Libraries , Contact Us ...
Researchers at the University of Chicago Medical Center have succeeded in growing HPV, the human papilloma virus-cause of genital warts and linked to carcinoma of the cervix-in cultured tissue for
0013] In another exemplary embodiment of the present disclosure, a method of transferring plant tissue explants from a first container having a first nutrient medium substrate adapted to provide nutrients to the plant tissue explants to a second container having a second nutrient medium substrate adapted to provide nutrients to the plant tissue explants is provided. The method comprising the steps of: (a) coupling a first plant tissue explant to a handheld tool, the handheld tool coupling the first plant tissue explant through a negative pressure present in an interior of the handheld tool; (b) generally separating the first plant tissue explant from the first nutrient medium substrate adapted to provide nutrients to the first plant tissue explant and from the first container; (c) locating the first plant tissue explant relative to the second nutrient medium substrate adapted to provide nutrients to the first plant tissue explant of the second container; and (d) uncoupling the first plant tissue ...
BNC Tissue Culture Workshop. - Hosted by Lisa Reece. Biosafety Research Engineer. Lab Director of BNC Bionanotech. Core Facilities. Classes will be held every Monday, Wednesday, and Friday 2-3 PM. Start Date: Monday,Feb. 10,2014 End Date: Wednesday, Feb. 26,2014. At the Birck Nanotechnology Center. Please contact either Lisa Reece at [email protected] Nancy Black at [email protected] for more information!. Deadline to register will be February 5, 2014.. THIS WORKSHOP IS FOR ENGINEERS AND OTHERS WHO WANT TO LEARN BASIC TISSUE CULTURE TECHNIQUES.. GREAT SKILL/TECHNIQUE TO HAVE ON YOUR CV FOR BIONANO AND BIOENGINEERING FIELDS!. ...
An increasing amount of very useful quantitative evidence from health care research is available to practitioners. New research findings continually expand the knowledge base of what does more good than harm for patients, and institutional forces, both professional and financial, […]
Cell culture dishes / petri dishes: We focused on improved handling and stacking performance of the cell culture dishes to ensure a new level of safe
Over 30 years of experience in developibg and manufacturing diverse tissue culture portfolio, includes Sera, Media, Stem Cell Products, Cytogenetics, and more
This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male.
This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male.
The SciFlow™ 1000 System is an in vitro fluidics culture system that establishes gradients between cell culture wells for monitoring downstream metabolite effects in a 96-well format. The system mimics in vivo-like conditions, enabling the evaluation of a compounds effect in a physiologically relevant manner
Abstract Trophozoites and cysts of an Acanthamoeba were repeatedly isolated from the corneal scrapings of a patient suffering from acute ulceration of the right eye. The ameba was cloned and cultivated axenically in a proteose peptone-yeast extract-glucose medium. At a later date the organism was identified as A. polyphaga on the basis of its morphologic characteristics, especially those of cysts. Experimental studies on the in vitro interactions of this organism with monkey kidney tissue culture (Vero line) and its pathogenicity to mice indicated that it was a low virulent strain. When large numbers of amebae (25,000+) were inoculated into Vero cell cultures, cytopathic effects (CPE) were noticed within 5 to 6 days. The CPE consisted of cell shrinkage, nuclear pycnosis, and discontinuity of cell sheet, and the cell culture was totally destroyed in 8 to 10 days. When 20,000+ amebae were instilled intranasally into each of 20 2-week-old mice, only 1 mouse died, on the 28th day. Amebae were isolated from
To enable embryo collection, manipulation, and transfer techniques, we offer a wide selection of mouse embryo media and reagents including M2, modified M16, FHM and proprietary KSOM media formulations. All of our media are tested on mouse embryos and manufactured using the highest quality raw materials available.

Mouse Embryo Validated Products
Species, Research Grants, Research Topics, Publications, Genomes and Genes, Scientific Experts about indirect fluorescent antibody technique
Quantitative aspects of metal ion binding to certain transfer RNA anticodon loop modified nucleosides.: Magnesium and manganese ions bind strongly to the unusua
A novel in vitro culture system of organotypic human skin explants interfacing with external fixator pins is presented. The system was used to observe changes in skin morphology on the skin at the pin
Infection and replication of HIV-1 have been analysed in various tissue culture systems including primary cells. These studies mostly applied ensemble measurements, and few analyses of individual events at high spatial and temporal resolution are currently available. Cellular and viral macromolecular complexes are involved at all stages of HIV-1 replication, and many individual factors and their respective contributions have been identified. Surprisingly little is known about fundamental details of many steps in HIV-1 replication, however. Important examples include (i) virus attachment and entry at the plasma membrane or from within the endosome in different target cells, (ii) timing and mechanisms of capsid uncoating, (iii) localisation, structure and composition of the genome replication complex and its conversion into the pre-integration complex, and (iv) induction and control of innate immunity. Accordingly, the early phase is probably the most enigmatic stage of HIV-1 replication.. Within ...
Sprawdź ile zapłacisz za lek Imovax Rabies human diploid cell w aptece, znajdź tańsze zamienniki leku. Określ swoje uprawnienia i sprawdź jakie zniżki Ci przysługują.
Exosomes are specialized, nanometer‐sized extracellular vesicles of endosomal origin actively secreted into the extracellular space by a variety of cells under normal and pathological conditions
Let X be a subvariety of Pn defined over a number field and N(B) be the number of rational points of height at most B on X. There are then general conjectures of Manin on the asymptotic behaviour of N(B) when B goes to infinity. These conjectures can be studied using the Hardy-Littlewood method for non-singular complete intersections of high dimensions and by adelic harmonic analysis for varieties related to algebraic groups. But for most varieties there are no other methods available apart from sieve theory and determinant methods. The latter was first developed for affine plane curves in a paper of Bombieri-Pila and extended to projective varieties by Heath-Brown and myself. The goal of the p-adic version of this method is to show that the set of rational points of height at most B on X in a congruence class satisfy further equations if p is large enough compared to B. One can then proceed by induction with respect to dim(X) to obtain uniform upper estimates for N(B) like
Human term-placental culture techniques such as villous explant or dual perfusion are commonly used to study trophoblast function under control and experimentally manipulated conditions. We have compared trophoblast viability during perfusion and in
Buy Argos Technologies® Cell and Tissue Culture Plates and more from our comprehensive selection of Cell Culture Plates from Cole-Parmer
Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite.
Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite.
The CulturPlate is PerkinElmerlj Microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. The optimized well design and high quality plastics provides low background and crosstalk.. CulturPlates are uniquely engineered to be the same height for 96-, 384-, and 1536-well plates, and with extraordinary well flatness for ease in automation.. Leveraging years of assay and instrument experience in plate detection, PerkinElmer designs better Microplates for better performance that guarantees better results for all PerkinElmer applications.. ...
Dishes. Amoebae must have tissue culture treated bottom to attach to.. If you use a culture flask, you cant pick up the amoebae.. Culture flask (Krackeler 40-229331 Celltreat Polystyrene Tissue culture flask 50mL Triangular TC-treated 25cm2 Angled Vent $103.00/200). If you use culture dishes (Krackeler 40-229670 Tissue culture dish with lid Polystyrene 8mL 70 x 15mm 36.3cm2 With gripping ring $115.00/500), you can more easily see and handle them, but you risk spilling.. Bigger dishes for mass cultivation Krackeler 40-229620 Tissue culture dish with lid Polystyrene 16mL 100 x 15mm 55cm2 With gripping ring $103.00/300. ...
An introduction to cell culture, covering topics such as laboratory setup, safety, and aseptic technique. Youll also find basic methods for passaging, freezing, and thawing cultured cells. Start here if you are new to cell culture techniques or need to refresh your knowledge. Explore. ...
DHUKATE MADANMOHAN REVANSIDDHA At / post-Balwadi, E-mail: [email protected] Tal- Sangola, Phone No. 02187-218689 Dist: Solapur, 9970102974, .
TISSUE CULTURE PLANTS We are a leading manufacturer of tissue culture plants such as tissue culture lemon plants, pomegranate plant, banana tissue culture plants, seedless lemon and date palm trees from India. About 5% of these are Laboratory Furniture, 0% are Other Outdoor Furniture. Plant tissue culture lab production managar Resume. It is not uncommon, given that plant tissue culture is working with sterile living material, for there to be a loss of some degree of stock. CO2 Incubator / Cell Culture Incubator A cell culture incubator is designed to maintain a constant temperature and high humidity for the growth of tissue culture cells under a CO2 atmosphere. Plant Tissue Culture which has been around for decades, is a way to reproduce new plants from the mother tissue and is used as an alternative to cloning. It is highly recommended for laboratory courses in tissue culture and would be useful in research laboratories where tissue culture techniques are required. Those interested in all ...
In the recent years, tissue culture has emerged as a promising technique to obtain genetically pure elite populations under in vitro conditions. In vitro propagation also called micropropagation is in fact the miniature version of conventional propagation, which is carried out under aseptic conditions. The advent of in vitro tissue culture technique has offered a new approach to the morphogenetic investigations. In the present study an attempt has been made to formulate a suitable Protocol for efficient micropropagation from seedling of axillary shoot buds explants of Sapindus emarginatus Vahl ...
In 1974 Rudolf Jaenisch created a transgenic mouse by introducing foreign DNA into its embryo, making it the worlds first transgenic animal.[14][15] However it took another eight years before transgenic mice were developed that passed the transgene to their offspring.[16][17] Genetically modified mice were created in 1984 that carried cloned oncogenes, predisposing them to developing cancer.[18] Mice with genes knocked out (knockout mouse) were created in 1989. The first transgenic livestock were produced in 1985[19] and the first animal to synthesise transgenic proteins in their milk were mice,[20] engineered to produce human tissue plasminogen activator in 1987.[21]. In 1983 the first genetically engineered plant was developed by Michael W. Bevan, Richard B. Flavell and Mary-Dell Chilton. They infected tobacco with Agrobacterium transformed with an antibiotic resistance gene and through tissue culture techniques were able to grow a new plant containing the resistance gene.[22] The gene gun ...
He is a Fellow of the Alexander von Humboldt Foundation, an alumnus of the German Academic Exchange Service (DAAD). He holds Certificate in Tissue Culture Techniques (United Nations University, Tokyo), Certificate in Cytogenetics (IITA, Ibadan) and Certificate in Food and Plant Analysis (United Nations/CSIR, India). He served as PhD external examiner for University of Fort Hare, South Africa (2007-2013) and project assessor for the Danish Government (2006-2009) and external examiner to many universities in Nigeria. He is a Fellow of the College of Research Associates (CRA), United Nations University, Tokyo, Japan. He had postdoctoral research experience at the United Nations University, CFTRI, Mysore-India from 2005-2006. He also worked as an Alexander von Humboldt (AvH) Foundation Research Fellow at the University of Bonn-Germany in 2006-2008. He was at several times a Visiting Professor at the University of Bonn-Germany (2009, 2011, 2014, 2017). He worked as Visiting Professor at the ...
Buy the Paperback Book Quantitative Aspects of Risk Assessment in Chemical Carcinogenesis by J. Clemmesen at Indigo.ca, Canadas largest bookstore. + Get Free Shipping on Health and Well Being books over $25!
Thorpe, Trevor A. (Herausgeber); Yeung, Edward C. (Herausgeber) Plant Embryo Culture Methods and Protocols Reihe: Methods in Molecular Biology 710 9781617379871 1617379875 A11399202 A11399202
Breast cancer is the most common malignancy in women, with 80% of patients suffering from invasive tumors and metastatic disease. Metastasis is a process where breast cancer cells disseminate from the mammary gland, invade through the extracellular matrix (ECM), intravasate, colonize a distant site such as the bone marrow (BM), and establish growing metastases. Further complicating the metastatic process is the requirement for permissive microenvironment at the secondary site to allow its colonization by the metastatic cells. It has been proposed that cancer stem cells (CSCs) with the ability to self-renew, can drive the growth and spread of the tumor. However, the contribution of the breast CSCs (B-CSCs) to metastatic disease is not completely understood. The purpose of this study is to develop a novel 3-dimensional (3-D) tissue culture model to delineate the molecular events responsible for tumor metastasis and to determine whether B-CSCs are responsible for metastatic spread. We developed a ...
Learning Objectives Describe the benefits of immunofluorescent antibody assays in comparison to nonfluorescent assays Compare direct and indirect flu
Vitrolife is committed to improving pregnancy rates. This is why we provide an unbroken chain of quality products, securing the results in every step during the whole IVF-treatment. Only Vitrolife can guarantee every link in this chain. With equally devoted clinics we reach success. Together. All the way.
Get information, facts, and pictures about tissue culture at Encyclopedia.com. Make research projects and school reports about tissue culture easy with credible articles from our FREE, online encyclopedia and dictionary.
The Raj laboratory occupies approximately 2000 ft2 on the South Campus and includes state-of-the-art equipment and research facilities, including a dedicated RNA suite and tissue-culture facilities.
Nicole Gentile, PhD Candidate, provides an overview of basic microbiology and the concepts involved, including the bacterial growth curve and classifying organisms based on morphologies. This lecture describes blood, urine and skin/soft tissue cultures, focusing on the types of media, sample collection processes, culture procedures, as well as speciation and susceptibility testing. Basic staining procedures,…
Our Chief Scientific Officer, Dr. Steve Navran, will be presenting on his own work and collaborative work using our Rotary Cell Culture System. ...
Neural Plasticity is a peer-reviewed, Open Access journal that publishes articles related to all aspects of neural plasticity, with special emphasis on its functional significance as reflected in behavior and in psychopathology. Neural Plasticity publishes research and review articles from the entire range of relevant disciplines, including basic neuroscience, behavioral neuroscience, cognitive neuroscience, biological psychology, and biological psychiatry.
The new technique could not only enhance research of prostate biology and cancer, but also hasten the creation of individualized medicines for prostate cancer patients, the investigators say. Previous attempts to culture live prostate tissues resulted in poor viability and lost "tissue architecture," the researchers note, making them less than useful for research or therapy development. "Our technique could help scientists more accurately predict how living prostate tissues respond to therapy," says Marikki Laiho, M.D., Ph.D., director of the division of Molecular Radiation Sciences at Johns Hopkins. "It holds promise for testing anticancer drugs that work best." For the study, published in the November 1 issue of Cancer Research, the scientists refined their multistep tissue culture technique and performed experiments to test the tissues viability and utility in research. Laiho worked with Stanford University researcher Donna Peehl, Ph.D., to pilot the technique in a research project completed ...
|p||strong|Technical Advantage: |/strong| Product manufactured under industry-leading standards for low endotoxin content and superior results.|br /| Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.  This research indicated that additions made to BME aided cell propagation.  MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells.  When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts.  This formulation contains Earles salts and does not contain L-glutamine and phenol red.|br /| -  Complete Certificate of Analysis available for each production lot, along with full formulation information|/p|
An intriguing theory proposed by John Martin, M.D., Ph.D. is that many autistic children have acquired a stealth virus infection through vaccination. Because of the unusual make-up of the stealth virus, it eludes the immune system, leading to chronic infection. If your child actually does better on antibiotics than off of them, this may be an avenue worth pursuing. According to Dr. Martin, "When live SV-40 virus was found in formulin treated polio virus vaccine, a switch was made to use kidney cells from African Green Monkeys using a live (attenuated) strain of poliovirus. Kidney cultures from all 12 monkeys tested grew out simian cytomegalovirus (SCMV). Sequencing studies done by Dr. Martin on the stealth virus indicate that it originated in SCMV but the CDC and FDA have exhibited an unwillingness to have their vaccine safety procedures reviewed. Dr. Martin writes an in-depth account of the cover-up by government agencies of the contamination of vaccines even though SCMV is still being found in ...
Carrots may help prevent leukemia, according to a study published in the November 2011 issue of the "Journal of Medicinal Food." In the tissue culture study, 72 hours of exposure to carrot juice extract promoted early cell death in human leukemia cells.Lately I heard of another few people, my friends friends, who are diagnosed withCANCER. As I sat and tried to recall my many friends who had suffered from various cancer, there are 24 of them! Sadly, 20 of them eventually died a very painful death not too long after diagnosis despite all the latest cancer treatments.. ...
PRIME-XV® Mouse Hematopoietic Cell Basal Medium was developed in a collaboration program with a leading stem-cell research facility in the USA to fulfill the need for a serum-free, cost-effective medium for in vitro culture and expansion and self-renewal of murine hematopoietic progenitor cells (mHPCs).. PRIME-XV Mouse Hematopoietic Cell Basal Medium is:. ...
Medical Decision Analysis Clearly, there is more to clinical decision making than simply collecting numbers that measure treatment effects. Reports of treatment effects in randomized, controlled trials are important starting points that help determine whether a treatment has merit in […]
Figure 4. Protective effect of catalase on HREC outgrowths. Protective effect of catalase on H2O2 induced cytotoxicity in 6 days HREC outgrowths. A: HREC aggregate grown in basal tissue culture medium shows normal sprouting after 6 days. B: HREC aggregate in medium supplemented with 1 mM H2O2, demonstrating that oxidative stress blocks sprouting. C: HREC aggregate grown in medium + 1 mM H2O2 in presence of catalase administered through an AnoporeTM filter (starting concentration in the upper compartment: 400 U/ml). The sprouts develop normally, showing that filter administered catalase can exert a protective effect on endothelial cells against oxidative stress induced injury. Phase contrast micrographs. Original magnifications: x80. D: SigmaScan Pro quantification of the outgrowth pattern illustrated in A.. ...
Cell-Aggregation, Cytotoxicity-Tests-Immunologic, Endotoxins, Fibrosarcoma: im, Lymphoma: im, Macrophages: de, im, Mice, Neoplasms-Experimental: im, Poly-I-C, Spleen: im, Tissue-Culture. ...
Solution Purchase Prescription Dirtop Free CARCINOMA TISSUE-CULTURE use OCULOCEREBRORENAL- SYNDROME Japan , Purchase Erectimax 25mg Albany, Online Delgra 100mg Jackson
For this procedure, the cells are first swollen in a buffered hypo‐osmotic medium containing potassium chloride, magnesium acetate and dithiothreitol to render the plasma membrane more susceptible to subsequent homogenization
Revision as of 05:21, 9 November 2015 by Wev012000 (Talk , contribs) (Created page with {, class=wikitable sortable collapsible ,- ! ID ! Icon ! Cell Culture Dish ! D N A Sampler ! Release Version ,- , 1fC , Image:Cell_culture_dish.png,link=Cell Culture Dish...) ...
Minimum Essential Medium (MEM) was developed by Harry Eagle, and is one of the most widely used of all synthetic cell culture media. It is designed for a wide range of species and cells.. ...
**9.5367431640625E-7**

Cardiovascular Tissue Engineering Laboratory Research Team - Ottawa Heart InstituteCardiovascular Tissue Engineering Laboratory Research Team - Ottawa Heart Institute

New culture techniques are being used to grow and expand the number of these cells and ways of re-delivering them into the ... Clusters of CD34+ cells expressing integrin α5 (green) after culture on a collagen matrix ...
more infohttps://www.ottawaheart.ca/research-team/cardiovascular-tissue-engineering-laboratory

Animal Cell Culture Techniques | SpringerLinkAnimal Cell Culture Techniques | SpringerLink

Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological ... Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological ... industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture. ... Colon Monoclonal Antibodies cell cell culture cell cycle cell differentiation cells cytokine gene expression gene therapy ...
more infohttps://link.springer.com/book/10.1007%2F978-3-642-80412-0

Plant Tissue Culture Techniques for Breeding | SpringerLinkPlant Tissue Culture Techniques for Breeding | SpringerLink

Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been ... Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been ... Bridgen M.P., Van Houtven W., Eeckhaut T. (2018) Plant Tissue Culture Techniques for Breeding. In: Van Huylenbroeck J. (eds) ... Embryo culture Micropropagation Plant tissue culture Protoplast Somaclonal variation This is a preview of subscription content ...
more infohttps://link.springer.com/chapter/10.1007%2F978-3-319-90698-0_6

culture techniques Protocols and Video...'culture techniques' Protocols and Video...

A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering, Isolation and Culture of ... culture techniques include Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow, ... Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model, Endothelialized Microfluidics for ... Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice, Evaporation-reducing Culture Condition ...
more infohttps://www.jove.com/keyword/culture+techniques

Urine culturing technique in febrile infants.  - PubMed - NCBIUrine culturing technique in febrile infants. - PubMed - NCBI

Urine culturing technique in febrile infants.. Bonadio WA.. Abstract. During the period from July to November 1984, 265 ... The technique utilized for collecting urine for culture in infants has a major impact on the incidence of urinary tract ... Eighty-seven percent of infants with a positive urine culture result collected by bag technique revealed a mixture of more than ... Over the next four months, catheterization and suprapubic aspiration techniques increased from 0 to 72%; bag technique ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/3615238?dopt=Abstract

TNO Repository search for: subject:Cell Culture TechniquesTNO Repository search for: subject:'Cell Culture Techniques'

Cell Culture Techniques · Cell Differentiation · Cells, Cultured · Coculture Techniques · Humans · Intervertebral Disk · Stem ... sandwich culture · Animals · Biological Transport, Active · Cell Culture Techniques · Cell Survival · Cells, Cultured · ... Cell Culture Techniques · Cell Proliferation · Cell Survival · Cells, Cultured · Culture Media, Conditioned · ... Amides · Capillaries · Cell Culture Techniques · Cell Movement · Cells, Cultured · Cytoskeleton · Endothelial Growth Factors · ...
more infohttps://repository.tudelft.nl/search/tno/?q=subject:%22Cell%20Culture%20Techniques%22

Biology | Free Full-Text | Advanced Cell Culture Techniques for Cancer Drug DiscoveryBiology | Free Full-Text | Advanced Cell Culture Techniques for Cancer Drug Discovery

These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor ... Recent studies have demonstrated the potential of utilizing 3D cell culture models in drug discovery programs; however, it is ... Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely ... a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two- ...
more infohttp://mdpi.com/2079-7737/3/2/345

Learn protein and cell culture techniques - Flinn FoundationLearn protein and cell culture techniques - Flinn Foundation

... and cell culture. Prerequisite: Permission of Instructor. Students should have had college biology and chemistry (BIO 181 or ...
more infohttps://www.flinn.org/learn-protein-and-cell-culture-techniques/

Sand Culture Technique and Biochemical Methods by Sinha Pratima, Gopal Rajeev | WaterstonesSand Culture Technique and Biochemical Methods by Sinha Pratima, Gopal Rajeev | Waterstones

Buy Sand Culture Technique and Biochemical Methods by Sinha Pratima, Gopal Rajeev from Waterstones today! Click and Collect ... Sand Culture Technique and Biochemical Methods (Paperback). Sinha Pratima (author), Gopal Rajeev (author) Sign in to write a ...
more infohttps://www.waterstones.com/book/sand-culture-technique-and-biochemical-methods/sinha-pratima/gopal-rajeev/9783659695445

Use of cell separation and short-term culture techniques to study erythroid cell development.  - PubMed - NCBIUse of cell separation and short-term culture techniques to study erythroid cell development. - PubMed - NCBI

Use of cell separation and short-term culture techniques to study erythroid cell development.. Glass J, Lavidor LM, Robinson SH ... These techniques have provided a model system for the study of erythroid cells at different stages of maturation isolated from ... In short-term culture, erythropoietin stimulated proliferation of pronormoblasts and basophilic normoblasts but probably not ... and separation of the residual nucleated erythroid cells as a function of size by the velocity sedimentation technique. The ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/1174706?dopt=Abstract

ANTI-RETICULAR IMMUNE SERUM: ITS ACTION DEMONSTRATED BY TISSUE CULTURE TECHNIQUE | ScienceANTI-RETICULAR IMMUNE SERUM: ITS ACTION DEMONSTRATED BY TISSUE CULTURE TECHNIQUE | Science

ANTI-RETICULAR IMMUNE SERUM: ITS ACTION DEMONSTRATED BY TISSUE CULTURE TECHNIQUE Message Subject. (Your Name) has forwarded a ...
more infohttp://science.sciencemag.org/content/100/2603/456

Alexis Carrels Tissue Culture Techniques | The Embryo Project EncyclopediaAlexis Carrel's Tissue Culture Techniques | The Embryo Project Encyclopedia

... was one of the pioneers in developing and modifying tissue culture techniques. The publicized work of Carrel and his associates ... Alexis Carrels Tissue Culture TechniquesAlexis Carrel, the prominent French surgeon, biologist, and 1912 Nobel Prize laureate ... Although the serial culturing technique could extend the life span of the cultures, Carrel noticed that the size of the cells ... Thus Carrels legacy had a mixed influence on the development of tissue culture techniques, which have been widely used in the ...
more infohttps://embryo.asu.edu/pages/alexis-carrels-tissue-culture-techniques

Culture Techniques and Their Applications to Studies of Vascular Smooth Muscle | Clinical Science | Portland PressCulture Techniques and Their Applications to Studies of Vascular Smooth Muscle | Clinical Science | Portland Press

Culture Techniques and Their Applications to Studies of Vascular Smooth Muscle Julie H. Campbell Julie H. Campbell ... Julie H. Campbell, Gordon R. Campbell; Culture Techniques and Their Applications to Studies of Vascular Smooth Muscle. Clin Sci ... Mucin biosynthesis and secretion in tracheal epithelial cells in primary culture Biochem J (December, 2000) ...
more infohttps://portlandpress.com/clinsci/article-abstract/85/5/501/76087/Culture-Techniques-and-Their-Applications-to?redirectedFrom=fulltext

Culture Techniques and Their Applications to Studies of Vascular Smooth Muscle | Clinical Science | Portland PressCulture Techniques and Their Applications to Studies of Vascular Smooth Muscle | Clinical Science | Portland Press

Culture Techniques and Their Applications to Studies of Vascular Smooth Muscle Julie H. Campbell Julie H. Campbell ... Julie H. Campbell, Gordon R. Campbell; Culture Techniques and Their Applications to Studies of Vascular Smooth Muscle. Clin Sci ... Mucin biosynthesis and secretion in tracheal epithelial cells in primary culture Biochem J (December, 2000) ...
more infohttps://portlandpress.com/clinsci/article-abstract/85/5/501/76087/Culture-Techniques-and-Their-Applications-to

Cell culture techniques essential for toxicity testing of inhaled mate by Shahnaz Bakand"Cell culture techniques essential for toxicity testing of inhaled mate" by Shahnaz Bakand

Advances inin vitrocell culture technology may provide some of the answers. Regulatory toxicologists and health and safety ... Recent innovations in human cell culture exposure and test systems has allowed the development ofin vitro assay systems that ... Innovative in vitro exposure techniques that have been developed to provide direct exposure of human lung cells to inhaled ... Cell culture techniques essential for toxicity testing of inhaled materials and nanomaterials in vitro ...
more infohttps://ro.uow.edu.au/sspapers/3081/

New cell culture technique paves the way for tailor-made cancer treatmentsNew cell culture technique paves the way for tailor-made cancer treatments

The new technique is more than three times as effective as previous methods. ... New cell culture technique paves the way for tailor-made cancer treatments. ... The new technique is more than three times as effective as previous methods (Expansion of CTCs from early stage lung cancer ... This culture method gives clinicians a way to study each patient s cancer much earlier and much more frequently, Nagrath said. ...
more infohttps://www.nanowerk.com/nanotechnology-news/newsid=38533.php

IDEALS @ Illinois: Browse Illinois Research and Scholarship (Open Community) by Subject Cell culture techniquesIDEALS @ Illinois: Browse Illinois Research and Scholarship (Open Community) by Subject "Cell culture techniques"

Browse Illinois Research and Scholarship (Open Community) by Subject "Cell culture techniques". ... Browse Illinois Research and Scholarship (Open Community) by Subject "Cell culture techniques". Welcome to the IDEALS ... Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice  ... We have developed a reliable protocol for the serum-free dissociation and culture of spiral ganglion neurons from adult mice, ...
more infohttps://www.ideals.illinois.edu/handle/2142/9/browse?value=Cell+culture+techniques&type=subject

Animal Cell Culture Techniques (Springer Lab Manuals) - 9783540630081 | SlugBooksAnimal Cell Culture Techniques (Springer Lab Manuals) - 9783540630081 | SlugBooks

Compare cheapest textbook prices for Animal Cell Culture Techniques (Springer Lab Manuals), - 9783540630081. Find the lowest ... Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological ... industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture. Hide ... In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: ...
more infohttps://www.slugbooks.com/9783540630081-animal-cell-culture-techniques-springer.html

Recent Advances in Lichenology: Modern Methods and Approaches in Lichen Systematics and Culture Techniques, Volume 2 book by...Recent Advances in Lichenology: Modern Methods and Approaches in Lichen Systematics and Culture Techniques, Volume 2 book by...

Recent Advances in Lichenology: Modern Methods and Approaches in Lichen Systematics and Culture Techniques, Volume 2 has 2 ... Modern Methods and Approaches in Lichen Systematics and Culture Techniques, Volume 2 by Dalip Kumar Upreti (Editor), Pradeep K ... Advanced culture techniques have expanded the pharmacological applications of lichens, which was formerly restricted due to ... Recent Advances in Lichenology: Modern Methods and Approaches in Lichen Systematics and Culture Techniques, Volume 2. by Dalip ...
more infohttps://www.alibris.com/Recent-Advances-in-Lichenology-Modern-Methods-and-Approaches-in-Lichen-Systematics-and-Culture-Techniques-Volume-2/book/29651148?qsort=p

Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella...Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella...

2014)‎. Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/ ... Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella ...
more infohttp://apps.who.int/iris/handle/10665/208735?locale-attribute=fr&

Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella...Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella...

2014)‎. Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/ ... Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella ...
more infohttp://apps.who.int/iris/handle/10665/208735?locale-attribute=zh&

Workshop: Introduction to Mammalian Cell Culture Techniques, Apr. 6-7 »  Interdisciplinary Center for Biotechnology Research »...Workshop: Introduction to Mammalian Cell Culture Techniques, Apr. 6-7 » Interdisciplinary Center for Biotechnology Research »...

The major topics are: maintaining mammalian cells in culture (aseptic technique, thawing, counting, expanding, freezing) and ... Workshop: Introduction to Mammalian Cell Culture Techniques, Apr. 6-7. Published: March 22nd, 2016 ... This workshop is designed for those new to mammalian cell culture or needing a refresher course on the basics. The workshop ...
more infohttp://www.biotech.ufl.edu/workshop-introduction-to-mammalian-cell-culture-techniques-apr-6-7/

Mycoplasma Testing Market Analysis By Product, By Technology (PCR, ELISA, Direct Assay, Microbial Culture Techniques), By...Mycoplasma Testing Market Analysis By Product, By Technology (PCR, ELISA, Direct Assay, Microbial Culture Techniques), By...

Mycoplasma Testing Market Analysis By Product, By Technology (PCR, ELISA, Direct Assay, Microbial Culture Techniques), By ... Mycoplasma contamination of cell culture is a growing concern for most of the research scientists for decades. Mycoplasma ...
more infohttps://www.reportlinker.com/p04838565/Mycoplasma-Testing-Market-Analysis-By-Product-By-Technology-PCR-ELISA-Direct-Assay-Microbial-Culture-Techniques-By-Application-Cell-Line-Virus-End-of-Production-Cells-Testing-By-End-use-And-Segment-Forecasts.html

Comparison of Conventional Broth Blood Culture Technique and Manual Lysis Centrifugation Techniaue for Detection of FungemiaComparison of Conventional Broth Blood Culture Technique and Manual Lysis Centrifugation Techniaue for Detection of Fungemia

Home » Comparison of Conventional Broth Blood Culture Technique and Manual Lysis Centrifugation Techniaue for Detection of ... Comparison of Conventional Broth Blood Culture Technique and Manual Lysis Centrifugation Techniaue for Detection of Fungemia. ... Blood culture is currently the gold standard for diagnosis of typhoid fever, but it is time-consuming and takes several days ... The author reflects on problems arising due to advent of automated blood culture systems such as BacT/Alert from bioMérieux, ...
more infohttp://connection.ebscohost.com/c/articles/36333553/comparison-conventional-broth-blood-culture-technique-manual-lysis-centrifugation-techniaue-detection-fungemia

Understanding the structure and function of human aquaporins: combining site-directed mutagenesis and mammalian cell culture...Understanding the structure and function of human aquaporins: combining site-directed mutagenesis and mammalian cell culture...

... combining site-directed mutagenesis and mammalian cell culture techniques to elucidate the key residues of AQP proteins ... This project will combine site-directed mutagenesis and mammalian cell culture techniques to elucidate the key residues of AQP ... combining site-directed mutagenesis and mammalian cell culture techniques to elucidate the key residues of AQP proteins ... combining site-directed mutagenesis and mammalian cell culture techniques to elucidate the key residues of AQP proteins ...
more infohttps://www.findaphd.com/phds/project/understanding-the-structure-and-function-of-human-aquaporins-combining-site-directed-mutagenesis-and-mammalian-cell-culture-techniques-to-elucidate-the-key-residues-of-aqp-proteins-required-for-these-processes/?p85560
**3.3223440647125**
**3.3226141929626**
  • To study the effects of a specific group of hormones (all ligands of the steroid/retinoid/thyroid hormone receptor superfamily) on the angiogenic process in humans, we have used a model system in which human microvascular endothelial cells from foreskin (hMVEC) are cultured on top of a human fibrin matrix in the presence of basic fibroblast growth factor and tumor necrosis factor-α. (tudelft.nl)
  • Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely characterizes the disease state have been undertaken by a number of laboratories around the world. (mdpi.com)
  • These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor cells. (mdpi.com)
  • In addition, a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two-dimensional monolayer conditions do not respond to cancer therapeutics/compounds in a similar manner. (mdpi.com)
  • The new capture and culture method changes this by providing a reliable way to get usable numbers of circulating tumor cells from even early-stage patients. (nanowerk.com)
  • Recent innovations in human cell culture exposure and test systems has allowed the development ofin vitro assay systems that are predictive, representative and suitable for toxicity screening of a diverse range of chemicals including airborne materials and nanomaterials (NMs). (edu.au)
  • The test has a simple procedure that combines parasite culture with a drug assay. (ajtmh.org)
  • In the first technique (TBG-RV-XLT4) samples were incubated at 43 °C in tetrathionate broth and then Rappaport-Vassiliadis broth before plating on XLT4 agar. (scielo.org.za)
  • We have developed a reliable protocol for the serum-free dissociation and culture of spiral ganglion neurons from adult mice, an important animal model for patients with post-lingual hearing loss. (illinois.edu)
  • There were discordant results for 60 samples, including 59 that were only culture-positive using the TBG-RV-XLT4 method, and one sample that was only culture-positive using the SEL-XLD method. (scielo.org.za)
  • 1997) as long as there is a predefined protocol for sample collection and culturing and the analysis and interpretation of results are used as a basis for determining whether there is a need for intervention and which specific actions should be taken (Sehulster et al . (scielo.org.za)
  • Therefore porcine lung and liver macrophages were isolated, and incubated with lipopolysaccharide (LPS) to initiate an acute inflammatory response, represented by the release of high amounts of tumour necrosis factor-α (TNF-α) into the culture medium. (tudelft.nl)
  • Experimentally, Carrel and Burrows adapted Harrison's hanging drop method of amphibian embryonic cells for wider applications and so that tissues could be cultured for a longer duration. (asu.edu)
  • This culture method gives clinicians a way to study each patient s cancer much earlier and much more frequently, Nagrath said. (nanowerk.com)
  • Cell populations highly enriched for the different stages of erythroid cell maturation were obtained by three sequential operations: harvesting of erythroid cells after induction of erythroid hyperplasia in the spleens of mice, elimination of the more mature erythrocytes by immunologic techniques, and separation of the residual nucleated erythroid cells as a function of size by the velocity sedimentation technique. (nih.gov)
  • Advances inin vitrocell culture technology may provide some of the answers. (edu.au)
  • Use of cell separation and short-term culture techniques to study erythroid cell development. (nih.gov)
  • These techniques have provided a model system for the study of erythroid cells at different stages of maturation isolated from a uniform source at one point in time. (nih.gov)
  • Electron microscopy shows cultured CTC cells (circled in red) growing on the chip device used in the study. (nanowerk.com)
  • Chabane D, Assani A, Bouguedoura N, Haïcour R, Ducreux G (2007) Induction of callus formation from difficile date palm protoplasts by means of nurse culture. (springer.com)
  • This technique has the potential to improve the understanding of pathologies at the vitreomacular interface and might be helpful in establishing anti-fibrotic treatment strategies. (uni-muenchen.de)
  • Routine, non-targeted sampling and culture of environmental samples collected from healthcare environments is not recommended by the Centers for Disease Control and Prevention (CDC) and other authorities (Sehulster et al . (scielo.org.za)