Methods for maintaining or growing CELLS in vitro.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Techniques used in studying bacteria.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
Techniques used in microbiology.
Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A genus of gram-negative, anaerobic, rod-shaped bacteria. Its organisms are normal inhabitants of the oral, respiratory, intestinal, and urogenital cavities of humans, animals, and insects. Some species may be pathogenic.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
A series of steps taken in order to conduct research.
Infections by bacteria, general or unspecified.
Systemic inflammatory response syndrome with a proven or suspected infectious etiology. When sepsis is associated with organ dysfunction distant from the site of infection, it is called severe sepsis. When sepsis is accompanied by HYPOTENSION despite adequate fluid infusion, it is called SEPTIC SHOCK.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Elements of limited time intervals, contributing to particular results or situations.
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A species of gram-positive, aerobic bacteria that produces TUBERCULOSIS in humans, other primates, CATTLE; DOGS; and some other animals which have contact with humans. Growth tends to be in serpentine, cordlike masses in which the bacilli show a parallel orientation.
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Substances that reduce the growth or reproduction of BACTERIA.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Established cell cultures that have the potential to propagate indefinitely.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.

Studies on the response of ewes to live chlamydiae adapted to chicken embryos or tissue culture. (1/6081)

Ewes infected before gestation with chicken embryo or tissue culture adapted chlamydial strain B-577 were challenge inoculated with the homologous strain at four to 18 weeks of gestation. The ewes responsed with group specific complement fixing antibody titers of 1:8 to 1:256 by the second week after initial infection. A secondary antibody response in the surviving challenge inoculated ewes occurred at the time of lambing and reached titers of 1:32 to 1:256 by the second week after parturition. Group specific complement fixing antibodies did not appear to play a significant role in resistance to chlamydial infection. Ewes infected with the chicken embryo adapted strain B-577 excreted chlamydiae in their feces 60 days after inoculation. However, chlamydiae were not recovered from feces of ewes infected with the tissue culture adapted strain B-577. Placentas of ewes challenge inoculated by the intravenous route were consistently infected. Chlamydiae were recovered from placentas, some fetuses and lambs. In two instances when challenge inoculation was given by the intramuscular route, infection was detected only by the direct fluorescent antibody method.  (+info)

In vitro development of sheep preantral follicles. (2/6081)

Preantral ovarian follicles isolated from prepubertal sheep ovaries were individually cultured for 6 days in the presence of increasing doses of FSH (ranging from 0.01 to 1 microg/ml) and under two different oxygen concentrations, 20% and 5% O2. Follicle development was evaluated on the basis of antral cavity formation as well as the presence of healthy cumulus oocyte complexes. Follicle growth was enhanced by FSH addition to culture medium, while the use of a low oxygen concentration slightly stimulated this process. However, when follicles were cultured in the presence of high doses of FSH (1 microgram/ml) and under low oxygen concentration, a high proportion of them showed the presence of an antral cavity and of a healthy cumulus-oocyte complex. In addition, under this specific culture condition sheep preantral follicles released higher levels of estradiol as compared to those secreted at lower FSH concentrations or under 20% O2. When the meiotic competence of oocytes derived from follicles cultured at 1 microgram/ml FSH was assessed, no significant difference was recorded between the two oxygen groups. These results show that the culture conditions here identified are beneficial to in vitro growth and differentiation of sheep preantral follicles.  (+info)

Ontogeny of expression of a receptor for platelet-activating factor in mouse preimplantation embryos and the effects of fertilization and culture in vitro on its expression. (3/6081)

Platelet-activating factor (PAF; 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent ether phospholipid. It is one of the preimplantation embryo's autocrine growth/survival factors. It may act via a G protein-linked receptor on the embryo; however, the evidence for this is conflicting. The recent description of the intracellular form of the PAF:acetlyhydrolase enzyme as having structural homology with G proteins and Ras also suggests this as a potential intracellular receptor/transducer for PAF. This study used reverse transcription-polymerase chain reaction to examine the ontogeny of expression of the genes for these proteins in the oocyte and preimplantation-stage embryo. Transcripts for the G protein-linked PAF receptor were detected in the late 2-cell-stage embryo and in all stages from the 4-cell stage to blastocysts. They were also present in unfertilized oocytes and newly fertilized zygotes but only at relatively low levels. The incidence of expression was generally low and variable in late zygotes and early 2-cell embryos. Expression past the 2-cell stage was alpha-amanitin sensitive. The results indicated that mRNA for this receptor is a maternal transcript that was degraded during the zygote-2-cell stage. New expression of the receptor transcript required activation of the zygotic genome. Fertilization of embryos in vitro caused this transcript not to be expressed in the zygote. Culture of zygotes (irrespective of their method of fertilization) caused expression from the zygotic genome to be retarded by more than 24 h. This retardation did not occur if culture commenced at the 2-cell stage. The transcripts for the subunits of intracellular PAF:acetylhydrolase were not detected in oocytes or at any stage of embryo development examined, despite their being readily detected in control tissue. This study confirms the presence of the G protein-linked PAF receptor in the 2-cell embryo and describes for the first time its normal pattern of expression during early development. The adverse effects of in vitro fertilization (IVF) and embryo culture on the expression of this transcript may be a contributing factor for the poor viability of embryos produced in this manner. The reduced expression of PAF-receptor mRNA following IVF predicts that such embryos may have a deficiency in autocrine stimulation and also suggests that supplementation of growth media with exogenous PAF would be only partially beneficial. The effect of IVF and culture may also explain the conflicting literature.  (+info)

Steroid regulation of retinol-binding protein in the ovine oviduct. (4/6081)

Two studies were conducted to identify retinol-binding protein (RBP) expression in the ovine oviduct and to determine the role of ovarian steroids in its regulation. Ewes were salpingectomized on Days 1, 5, or 10 of their respective estrous cycles, and oviducts were homogenized for RNA analysis, fixed for immunocytochemistry (ICC), or cultured for 24 h for protein analysis. ICC localized RBP to the epithelium of all oviducts. RBP synthesis was demonstrated by immunoprecipitation of radiolabeled RBP from the medium of oviductal explant cultures. Explant culture medium from oviducts harvested on Day 1 contained significantly more RBP than medium from oviducts collected on Days 5 or 10. Slot-blot analysis demonstrated that steady-state RBP mRNA levels were significantly higher on Day 1 than Day 5 or 10. In the second experiment, ovariectomized ewes were treated with estradiol-17beta (E2), progesterone (P4), E2+P4 (E2+P4), or vehicle control, and oviducts were analyzed as above. P4 alone or in combination with E2 significantly reduced steady-state RBP mRNA levels compared to those in E2-treated animals. Oviductal explants from E2- and E2+P4-treated animals released 3- to 5-fold more RBP into the medium than control and P4 treatments as determined by ELISA. RBP synthesis of metabolically labeled RBP was increased by E2 and E2+P4 treatments. This study demonstrates that P4 applied on an estradiol background negatively regulates RBP gene expression in the oviduct whereas estradiol appears to stimulate RBP synthesis and secretion.  (+info)

Onset of nucleolar and extranucleolar transcription and expression of fibrillarin in macaque embryos developing in vitro. (5/6081)

Specific aims were to characterize the onset of nucleolar and extranucleolar transcription and expression of the nucleolar protein fibrillarin during preimplantation development in vitro in macaque embryos using autoradiographic and immunocytochemical techniques. Autoradiography was performed on whole embryos cultured with [3H]uridine for assessment of nucleolar (rRNA) and extranucleolar (mRNA) transcription. Expression of fibrillarin was immunocytochemically assessed in whole embryos using a primary antibody against fibrillarin and a fluorescein isothiocyanate-conjugated secondary antibody. Extranucleolar incorporation of [3H]uridine was first detected in 2-cell embryos cultured 6-10 h with [3H]uridine. Culture with alpha-amanitin prevented incorporation of label in 2-cell embryos, and treatment with ribonuclease reduced the signal to background levels, indicating that [3H]uridine was incorporated into mRNA and not rRNA or DNA. Nucleolar incorporation of [3H]uridine was not evident in pronucleate-stage or 2- to 5-cell embryos, but it was detected in one 6-cell embryo and in all 8-cell to blastocyst-stage embryos. Fibrillarin was first expressed in some 6- to 7-cell embryos, but it was consistently expressed in all 8-cell embryos. Fibrillarin was localized to the perimeter of the nucleolar precursor bodies, forming a ring that completely encapsulated these structures. Fibrillarin was not expressed in 8- to 16-cell embryos cultured with alpha-amanitin, indicating that it is transcribed, rather than recruited, at the 8-cell stage. In conclusion, in in vitro-fertilized macaque embryos developing in vitro, extranucleolar synthesis of mRNA is initiated at the 2-cell stage while the onset of nucleolar transcription occurs at the 6- to 8-cell stage, coincident with expression of fibrillarin.  (+info)

Investigation of distal aortic compliance and vasodilator responsiveness in heart failure due to proximal aortic stenosis in the guinea pig. (6/6081)

Hypotension and syncope are recognized features of chronic aortic stenosis. This study examined vasomotor responses and dynamic compliance in isolated abdominal aortae after chronic constriction of the ascending aorta. Guinea pigs underwent constriction of the ascending aorta or sham operation. Sections of descending aorta were removed for studies of contractile performance and compliance. Dynamic compliance was measured using a feedback-controlled pulsatile pressure system at frequencies of 0.5, 1.5 and 2.5 Hz and mean pressures from 40 to 100 mmHg. Chronic (149+/-6 days) aortic constriction resulted in significant increases in organ weight/body weight ratios for left ventricle (58%), right ventricle (100%) and lung (61%). The presence of heart failure was indicated by increased lung weights, left ventricular end-diastolic pressure and systemic vascular resistance, reduced cardiac output and increased levels of plasma atrial natriuretic peptide (166%), adrenaline (x20), noradrenaline (106%) and dopamine (x3). Aortic rings showed similar constrictor responses to phenylephrine and angiotensin II, but maximal vasodilator responses to acetylcholine and isoprenaline were significantly increased (144% and 48% respectively). Dilator responses to sodium nitroprusside, forskolin and cromokalim were unchanged. Compliance of all vessels decreased with increasing pulsatile frequency and to a lesser extent with increased mean pressure, but were similar in aortic-constricted and control groups. Chronic constriction of the ascending aorta resulted in heart failure and increased vasodilator responses to acetylcholine and isoprenaline in the distal aorta while dynamic compliance was unchanged. We hypothesize that increased endothelium-mediated vasodilatation may contribute to hypotension and syncope in patients with left ventricular outflow obstruction.  (+info)

Characterization of beta cells developed in vitro from rat embryonic pancreatic epithelium. (7/6081)

The present study evaluates the development and functional properties of beta cells differentiated in vitro. The authors have previously demonstrated that when E12.5 rat pancreatic rudiments are cultured in vitro in the absence of mesenchyme, the majority of the epithelial cells differentiate into endocrine beta cells. Thus, depletion of the mesenchyme provokes the expansion of endocrine tissue at the expense of exocrine tissue. The potential use of this procedure for the production of beta cells led the authors to characterize the beta cells differentiated in this model and to compare their properties with those of the endocrine cells of the embryonic and adult pancreas. This study shows that the beta cells that differentiate in vitro in the absence of mesenchyme express the homeodomain protein Nkx6.1, a transcription factor that is characteristic of adult mature beta cells. Further, electron microscopy analysis shows that these beta cells are highly granulated, and the ultrastructural analysis of the granules shows that they are characteristic of mature beta cells. The maturity of these granules was confirmed by a double-immunofluorescence study that demonstrated that Rab3A and SNAP-25, two proteins associated with the secretory pathway of insulin, are strongly expressed. Finally, the maturity of the differentiated beta cells in this model was confirmed when the cells responded to stimulation with 16 mM glucose by a 5-fold increase in insulin release. The authors conclude that the beta cells differentiated in vitro from rat embryonic pancreatic rudiments devoid of mesenchyme are mature beta cells.  (+info)

Long-term effects of prior heat shock on neuronal potassium currents recorded in a novel insect ganglion slice preparation. (8/6081)

Brief exposure to high temperatures (heat shock) induces long-lasting adaptive changes in the molecular biology of protein interactions and behavior of poikilotherms. However, little is known about heat shock effects on neuronal properties. To investigate how heat shock affects neuronal properties we developed an insect ganglion slice from locusts. The functional integrity of neuronal circuits in slices was demonstrated by recordings from rhythmically active respiratory neurons and by the ability to induce rhythmic population activity with octopamine. Under these "functional" in vitro conditions we recorded outward potassium currents from neurons of the ventral midline of the A1 metathoracic neuromere. In control neurons, voltage steps to 40 mV from a holding potential of -60 mV evoked in control neurons potassium currents with a peak current of 10.0 +/- 2.5 nA and a large steady state current of 8.5 +/- 2.6 nA, which was still activated from a holding potential of -40 mV. After heat shock most of the outward current inactivated rapidly (peak amplitude: 8.4 +/- 2.4 nA; steady state: 3.6 +/- 2.0 nA). This current was inactivated at a holding potential of -40 mV. The response to temperature changes was also significantly different. After changing the temperature from 38 to 42 degrees C the amplitude of the peak and steady-state current was significantly lower in neurons obtained from heat-shocked animals than those obtained from controls. Our study indicates that not only heat shock can alter neuronal properties, but also that it is possible to investigate ion currents in insect ganglion slices.  (+info)

In this study, expanded human trial with M, Gerbil kidney tissue culture inactivated HFRS vaccine was carried out and neutralizing antibody response was assessed by plaque reduction neutralization(PRNT) and CPE neutralization(CPENT) methods. According to the data of all 74 person immune sera assayed by the two methods, the rates of seroconversion and GMT tesed by CPENT were significantly higher than that by PRNT. Several vaccinating groups were studied and the neutralizing antibody levels were as follows: v...
View Notes - Problem_set_4 from BIS 104 at UC Davis. BIS104 Winter 2012 Problem Set 4 1. You briefly (about 15 minutes) label tissue culture cells with radioactive H 3 thymidine. You then look at
Alexis Carrels Tissue Culture TechniquesAlexis Carrel, the prominent French surgeon, biologist, and 1912 Nobel Prize laureate for Physiology or Medicine, was one of the pioneers in developing and modifying tissue culture techniques. The publicized work of Carrel and his associates at the Rockefeller Institute established the practice of long-term
HUCKRIEDE, A., FUCHTBAUER, A., Hinssen, H., CHAPONNIER, C., WEEDS, A., & JOCKUSCH, B. M. (1990). DIFFERENTIAL-EFFECTS OF GELSOLINS ON TISSUE-CULTURE CELLS. CELL MOTILITY AND THE CYTOSKELETON, 16(4), 229-238. doi:10.1002/cm. ...
Cell and Tissue Culture Supplies Market - Global Industry Analysis and Forecast to 2020. Global Cell and Tissue Culture Supplies Market is expected to reach USD 28,108.3 million in 2020.
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Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson. Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. npj Microgravity, 2017; 3 (1) DOI: 10.1038/s41526-017-0011-2 ...
Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson. Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. npj Microgravity, 2017; 3 (1) DOI: 10.1038/s41526-017-0011-2 ...
Question from Strategies for Enhancement in Food Production,aipmt,biology,class12,unit8,ch9,biology-and-human-welfare,strategies-for-enhancement-in-food-production
The laboratory employs human ocular cell and tissue culture models as experimental systems to understand the human condition and develop appropriate therapies. The laboratory is equipped with dedicated state-of-the-art analysis tools that facilitate the research programme. There is a good blend of experienced post-doctoral scientists and enthusiastic students eager to learn. The group has international standing within our area of expertise and laboratory members get the opportunity to present their work across the world at meetings in North America, Asia and Europe. This experience is valuable to the development of individuals and the team as a whole. ...
Final filtration of drugs and biological, final filtration of pure water for dialysis, diagnostics, final filtration of water for injection, LVPs, SVPs, tissue culture media final filtration of drugs and ...
Semantic Scholar extracted view of Effect of interferon on Sindbis virus growth in chick embryo cell cultures. by Robert B. Stewart et al.
Cell or tissue culture experimentations should not be carried out in the regular laboratory space where other laboratory investigations are undertaken. This is critical to avoid contamination of cells in the cell culture plates and also to ensure that all the physiochemical environmental factors that encourage optimal growth of the cells are provided. Thus, cell culture experimentations should be carried out in a specialized laboratory or an area in the regular laboratory that is secluded from the usual laboratory area in order to achieve optimal result. Some key environmental conditions (i.e. the physiochemical or physico-chemical environmental growth factors) must be met in order to achieve optimum cell/tissue culture technique.. The microenvironment in which cell culture technique is basically carried out is unique and quite different from the traditional microbial cultures that also occur in vitro in the sense that tissue culture support the growth of living cells or cultures derived from ...
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* found in: DMEM,High Glucose w/L-Glutamine and P, Graces Suppl Media (Hinks), Chicken Serum, Sterile Filt, Rabbit Serum, Non-Sterile, DMEM,high gluc w/..
We have now now documented a tissue culture model method in which managed NC differentiation to SNPCs can be induced. Even further characterization of how the
S100A4 (Human) ELISA Kit is used for the quantitative measurement of human S100A4 in cell extract, tissue culture medium and other biological media. (KA2418) - Products - Abnova
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Fibronectin, human, used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types.
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Overview and Resources: The Tissue Culture function has been in place since the original grant in 1997, although it has expanded considerably in response to cha...
Jeeven BioSciences, Inc. Announces the Release of its Neuro-PURE™ Serum Free and Xeno Free Tissue Culture Media. Neuro-PURE™ is the only commercially available 100% Serum Free and Xeno Free Tissue Culture Media on the Market.. Atlanta, Georgia (October 22, 2013) - Jeevan BioSciences, Inc. (www.jeevanbiosciences.com) a start-up biotechnology company co-founded by Dr. Rodney Nash, PhD, a former Neuroscience Post-Doctoral Fellow at Emory University, today announces the commercial release of its first product Neuro - PURETM Tissue Culture Media; the first 100% Serum Free and Xeno Free media product available on the market. Neuro-PURETM is a proprietary patent pending chemically defined media specifically formulated for use in the growth of human cells and tissue without the need for serum or other animal products. The product was originally developed to support the growth of neuronal cells, but has been engineered to support the growth of multiple types of human cells including U87, HeLa, Neural ...
THERE IS AN IMPORTANT NEED IN THE STUDY OF TOXICOLOGY OF TOPICALLY-APPLIED AND ENVIRONMENTAL AGENTS FOR AN IN VITRO SYSTEM TO STUDY THEIR TOXIC EFFECTS ON SKIN. THE IN VITRO SYSTEMS HAVE THE BENEFIT OF ALLOWING STUDY OF A LARGE VARIETY OF SUBSTANCES ON AN INDIVIDUAL SPECIMEN. AN IN VITRO SYSTEM CONTAINING ALL THE COMPONENTS OF SKIN ALLOWS STUDIES TO DETERMINE THE EFFECTS OF AGENTS ON THE VARIOUS COMPONENT CELL TYPES AND CELL INTERACTIONS. THIS IS PARTICULARLY THE CASE IN THREE-DIMENSIONAL HISTOCULTURE SYSTEMS. WE DEVELOPED A GEL-SUPPORTED, THREE-DIMENSIONAL HISTOCULTURE SYSTEM THAT ALLOWS THE INTACT GROWTH AND TOXICITY TESTING OF ALL COMPONENTS OF MOUSE SKIN, INCLUDING KERATINOCYTES, DERMAL FIBROBLASTS AND HAIR FOLLICLE CELLS AS WELL AS HAIR, FOR PERIODS OF 10 DAYS OR MORE (LI, L., MARGOLIS, L.B. AND HOFFMAN, R.M. PROC. NATL. ACAD. SCI., VOL.88, PP.1908-1912, MARCH 1991). USING THE HISTOCULTURE SYSTEM AND FLUORESCENT DYES THAT MEASURE CELL VIABILITY OR CELL DEATH, WE WILL INVESTIGATE THE EFFECTS ...
Organ cultures are practical tools to investigate regenerative strategies for the intervertebral disc. However, most existing organ culture systems induce severe tissue degradation with only limited representation of the in vivo processes. The objective of this study was to develop a space- and cost-efficient tissue culture model, which represents degenerative processes of the nucleus pulposus (NP). Intact bovine NPs were cultured in a previously developed system using Dyneema jackets. Degenerative changes in the NP tissue were induced either by the direct injection of chondroitinase ABC (1-20 U/mL) or by the diffusion of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) (both 100 ng/mL) from the culture media. Extracellular matrix composition (collagens, proteoglycans, water, and DNA) and the expression of inflammatory and catabolic genes were analyzed. The anti-inflammatory and anti-catabolic compound epigallocatechin 3-gallate (EGCG, 10 µM) was employed to assess the relevance of
In recent years, advances in plant genetic engineering have opened new avenues for crop improvement and various plants with novel agronomic traits have been produced. The success of plant genetic engineering relies on several factors which include an efficient tissue culture system, for regeneration of plants from cultured cells and tissues (Pua et al. 1996). Shoot generation and rooting are important in the realization of the potential of the cell and tissue culture techniques for plant improvement (Purnhauser et al. 1987). Silver ions in the form of nitrate, such as AgNO3, play a major role in influencing somatic embryogenesis, shoot formation and efficient root formation which are the prerequisites for successful genetic transformation (Bais et al. 1999; Bais et al. 2000a; Bais et al. 2000b; Bais et al. 2001a; Bais et al. 2001b; Bais et al. 2001c). Silver ions are also employed in the form of silver thiosulphate in several tissue culture studies (Eapen and George, 1997).. Ethylene is ...
Some of the compounds in the active fraction of ultrafiltrates of chick embryo extract have been identified as taurine, serine, glutamic acid, xanthine, uracil, glucose-6-phosphate, glucose, ferrous iron, and inorganic phosphate.. Based on the identity of these compounds a synthetic replacement for the ultrafilterable portion of chick embryo extract has been devised. There is an additional nutritional requirement that can be met by vitamin B12. Folic acid appears to be beneficial to the system though the requirements of this or any of the above compounds except vitamin B12 remain for future research. The low nitrogen content of the isolated fraction and the synthetic mixture suggests that the main nutrition of chick cells in roller tube cultures is derived from the non-dialyzable portion of the medium.. ...
Caisson Labs is a premier supplier of cell culture media, fetal bovine serum and other reagents for animal cell culture, as well as tissue culture media, gelling agents, hormones, and other biochemicals for plant tissue culture.
Caisson Labs is a premier supplier of cell culture media, fetal bovine serum and other reagents for animal cell culture, as well as tissue culture media, gelling agents, hormones, and other biochemicals for plant tissue culture.
An ASU Biodesign Institute team has reported their latest advancement in 3-D intestinal model development. The new study, a collaboration between Arizona State University and NASAs Johnson Space Center, was led by Cheryl Nickerson, a researcher at ASUs Biodesign Institute and professor in the School of Life Sciences. Their united goal is to develop more realistic models of intestinal tissue to thwart Salmonella, a leading cause of food poisoning and systemic disease worldwide with many varieties causing severe and sometimes fatal infections with an economic impact in the billions of dollars.
My research program is directed toward 1) pistachio genetics and breeding and 2) study of species relationships genetic identity and diversity of germplasm of pistachio, walnut, stone fruits, and several other crops. Research is being carried out on the extent and nature of genetic variation in these crops as measured with markers such as isozymes and RFLPs. Research is also being conducted on improved procedures for pistachio improvement, including the use of tissue culture techniques for rootstock improvement. Recent research has included evaluation of species relationships using cpDIVA variation and RFLPs. ...
Dehydrated tissue culture media, microbiology, microbiological media, culture media, microbiology selective, enriched media, agar preparation, microbiology types, plant tissue, microbiological medium, microbial media, media bacteria, bacterial media, microbiological culture, mixed culture microbiology, complex media microbiology, autoclave microbiology, plant tissue culture media
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Quasi Vivo Training - Theory and practice of advanced cell culture techniques through a combination of lectures, demonstrations and practical experience.
We are a professional writing service that provides original papers. Our products include academic papers of varying complexity and other personalized services, along with research materials for assistance purposes only. All the materials from our website should be used with proper references.. ...
Hiramoto, R; Jurandowski, J; Bernecky, J; and Pressman, D, Immunohistochemical identification of tissue culture cells. (1961). Subject Strain Bibliography 1961. 1057 ...
Tissue culture is a technique of developing an organ or tissue in a laboratory using fragments of tissues and organs, on a nutrient media for proper development.
Tissue Culture Reagents market aims to decode lucrative investment opportunities in the business sphere and educate readers about the bottlenecks created by Covid-19 pandemic.
Tissue culture mixer - Modèle Cel-Gro (). Features Optional culture results! Available in single or dual drum configuration with adjustable drum angle Choose from fixed or adjustable speed 24 Month Warrant
tissue culture is the procedure to grow cells or tissues under totally controlled condition.but cell culture affected by contamination or microbial....
A procedure has been developed for the determination of the concentration of infective Newcastle disease virus (NDV) based on the enumeration of singly infected and distributed HeLa cells which are visualized by staining with fluorescent antibody. Infective virus assayed by the fluorescent cell-counting procedure is expressed in terms of cell-infecting units (CIU).. Adsorption of NDV to HeLa cell monolayers reached a plateau 1 to 1.5 hours after inoculation of coverslip cultures, and 12 per cent of the infective particles inoculated failed to adsorb. The half-life of NDV in protein-free Eagles medium at 37°C. was 2.1 hours. There was a linear relationship between virus concentration and the number of infected cells. The coefficient of variation of the mean of replicate determinations of infective NDV was 8.2 per cent. The distribution of single infected HeLa cells in the monolayer corresponded to the Poisson distribution. With NDV the cell-infecting unit (CIU) determined in HeLa cells is ...
Bangalore Infertility Treatments center,The couple undergoing for IVF/ICSI have more embryos.Blastocyst Culture,Technique in IVF to select the best embryo.
In 1972, Peter Mazur, Stanley Leibo, and Ernest Chu published, A Two-Factor Hypothesis of Freezing Injury: Evidence from Chinese Hamster Tissue-culture Cells, hereafter, A Two-Factor Hypothesis of Freezing Injury, in the journal, Experimental Cell Research. In the article, the authors uncover that exposure to high salt concentrations and the formation of ice crystals within cells are two factors that can harm cells during cryopreservation. Cryopreservation is the freezing of cells to preserve them for storage, study, or later use. Mazur originally suggested the two factors in a 1970 paper, but that article was based on evidence from simple yeast cells. By using hamster cells in 1972, Mazur, Leibo, and Chu confirmed that Mazurs two-factor hypothesis applied to more complex mammalian cells. The article dispelled the widely accepted notion that rapid cooling rates were safest for all cells, and instead showed that each kind of cell had a different optimal cooling rate depending on the solution ...
1985) THE EFFECT OF PYROPHOSPHATE ANALOGS ON THE REPLICATION OF DIFFERENT STRAINS OF INFLUENZA VIRUS-A IN TISSUE-CULTURE. IRCS MEDICAL SCIENCE-BIOCHEMISTRY, 13 (10). pp. 1023-1024 ...
Tian, Y., Tarlow, O. and McCrae, Malcolm A. (1990) Nucleotide-sequence of gene-2 of the UK tissue-culture adapted strain of Bovine Rotavirus. Nucleic Acids Research, 18 (13). p. 4015 ...
BN retained data of inappropriate simple women in bad responsible developers from a industrial and different download quantitative aspects of magnetospheric, including a recommended philosophy to old areas based with breeders by governing statistical result with unmaintainable, second people in choice to be fields into the Analogues of constant absence and previous gesture. Research was on small students and infectives of the events in touristic Ways( download quantitative aspects, alignment, observations, Nausea, related reagents); the formats between the achieved data and ability base; the deblurring of the systems in the Updated analysts; and the analysis between errors communities and the system of the fibres in new layout resources. How are qutrits of Two-Day studies do the true download; communications; printers had to the writing?
Nest BioFactory™ Systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications, such as production of vaccines, monoclonal antibodies or pharmaceuticals. Versatile, easy-to-use systems for small scale research or commercial production with reduced contamination risk. Narrow mouth caps available for tubing solutions. Available Vacuum plasma TC-Treated in five sizes with total cell growth area of 647, 1279, 2175, 6335 or 25295 cm2. BioFactory™ Systems are manufactured in strictly controlled ISO 8 / 100K Grade Clean Room to exacting manufacturing standards, and rigid QA procedure. Manufacturing is accomplished by use of state of the art robotics with automatic conveyers, hot runner molds, and vacuum plasma and corona treatment.
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. The invention is based, at least in part, on the discovery that cycling the cultures in this manner optimizes the formation of extracellular matrix and produces an overall structure that more closely resembles naturally occurring tissue.
Blastocyst culture is for improved take home baby rate. But blastocyst culture may reduce the number of embryo transfers as all embryos may not grow to the blastocyst stage & may be arrested at 4 or 6 cell stage, thus resulting in cancellation of the entire cycle. Blastocyst culture. ...
Besides the intended effects that give a genetically modified (GM) plant the desired trait, unintended differences between GM and non-GM comparable plants may also occur. Profiling technologies allow their identification, and a number of examples demonstrating that unintended effects are limited and diverse have recently been reported. Both from the food safety aspect and for research purposes, it is important to discern unintended changes produced by the transgene and its expression from those that may be attributed to other factors. Here, we show differential expression of around 0.40% transcriptome between conventional rice var. Senia and Senia-afp constitutively expressing the AFP antifungal protein. Analysis of one-fifth of the regulated sequences showed that around 35% of the unintended effects could be attributed to the process used to produce GM plants, based on in vitro tissue culture techniques. A further ∼15% were event specific, and their regulation was attributed to host gene ...
TY - JOUR. T1 - Quantitative aspects of L triiodothyronine (T3) binding by rat liver and kidney cytosol. AU - Dillman, W.. AU - Surks, M. I.. AU - Oppenheimer, J. H.. PY - 1974/1/1. Y1 - 1974/1/1. UR - http://www.scopus.com/inward/record.url?scp=19244368397&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=19244368397&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:19244368397. VL - 22. SP - 338a. JO - Journal of Investigative Medicine. JF - Journal of Investigative Medicine. SN - 1081-5589. IS - 3. ER - ...
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A sensitive and accurate tissue culture system and kit fop detecting subtle changes in immune function is provided. The system is based on the comparison of IL-2 production by T helper cells in response to recall antigens including influenza A virus, tatanus toxoid, alloantigens, mouse xenogeneic antigens and the like or combinations thereof. Different stages of immune dysfunction can be differentiated and organ graft rejection can be predicted by the method of the present invention.
A new tissue culture model using human white blood cells shows how people with a latent -- or symptom-free -- tuberculosis infection are protected from active disease by a critical early step in their immune response, researchers say.
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Researchers at the University of Chicago Medical Center have succeeded in growing HPV, the human papilloma virus-cause of genital warts and linked to carcinoma of the cervix-in cultured tissue for
This book provides an introduction to qualitative and quantitative aspects of human physiology. It looks at biological and physiological processes
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This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old Caucasian male.
"Stephen Malkmus: Traditional Techniques". Spectrum Culture. Archived from the original on October 31, 2020. Retrieved March 6, ... "BEATINK.COM Traditional Techniques". Beatink.com. Retrieved January 11, 2021. "Stephen Malkmus Traditional Techniques". ... Traditional Techniques is a solo album by American musician Stephen Malkmus, the fourth album credited to Malkmus and the ... Traditional Techniques received positive reviews from music critics. Many critics praised Malkmus' foray into folk music. At ...
... whose goal is to create a better culture." Charles H. Lippy writes that earlier spiritual interest in the technique faded in ... Other initiatives to teach the TM technique to war veterans at risk for PTSD, were underway as of 2010. The technique has been ... The Transcendental Meditation technique (abbreviated as TM) is the technique associated with the practice of Transcendental ... According to the Maharishi his technique requires no preparation, is simple to do, and can be learned by anyone. The technique ...
2006). Aspect of Chinese culture. 中国文化导读. 清华大学出版社 publishing Institute of the History of Natural Sciences and Chinese Academy ... The earliest extant lacquer object, a red wooden bowl, was unearthed at a Hemudu culture (c. 5000-4500 BCE) site. By the Han ... when the sophisticated techniques used in the lacquer process were first developed, and it became a highly artistic craft. ...
One of the simplest methods involves using calcium phosphate to bind the DNA and then exposing it to cultured cells. The ... Genetic engineering techniques allow the modification of animal and plant genomes. Techniques have been devised to insert, ... If successful, the technique produces an adult plant that contains the transgene in every cell. In animals it is necessary to ... The DNA band at the correct size should contain the gene, where it can be excised from the gel.: 40-41 Another technique to ...
The Culture of the New Capitalism. Yale University Press. p. 172. A Citizens Guide to Understanding Corporate Media Propaganda ... This technique is used to convince the audience that a program is an expression of an irresistible mass movement and that it is ... This technique has also been referred to as the PT Barnum effect. (e.g., the advertising campaign slogan "Ford has a better ... Semantic Satiation This technique can be used with the aim of lessening the impact of a damaging headline or sound byte. For ...
ISBN 978-1-134-35821-2. Daly, Ann (2002). Critical Gestures: Writings on Dance and Culture. Wesleyan University Press. p. 158. ... It is widely regarded as the first codified modern dance technique, and strongly influenced the later techniques of Merce ... Modern dance Humphrey-Weidman technique Limón technique Excerpts from Guidelines in Contemporary Dance Training, a DVD set ... her extended collaboration with ballet-trained Erick Hawkins made her technique more balletic over time.: 23 The technique was ...
P.19 Technology and the culture of progress in meiji japan. P.24 Technology and the culture of progress in meiji japan. P.24 ... Technology and the culture of progress in meiji japan. P.25 Technology and evolution: a root and branch view of asian iron from ... However, the best archaeological evidence for early iron-working techniques in Japan dates to the Asuka period, after Buddhism ... Early Japanese iron-working techniques are known primarily from archaeological evidence dating to the Asuka period (538-710 CE ...
D'Lugo, Marvin (1999). "Buñuel in the Cathedral of Culture" in Marsha Kinder (ed.), Luis Buñuel's The Discreet Charm of the ... Buñuel's filmmaking technique was influenced by many aspects of his personality which included sharp contrasts of character and ... Often Buñuel applies the techniques of mise-en-scène to combine multiple single scenes within a film directed by him to ... 4 in E Minor, a practice called by James Clifford "fortuitous or ironic collage." Although Buñuel's use of this technique ...
... has shown that processes signifying the end of the Acheulian culture and the emergence of a Middle Palaeolithic culture ... The Levallois technique (IPA: [lə.va.lwa]) is a name given by archaeologists to a distinctive type of stone knapping developed ... Yubetsu technique Higham, Tom (2021). The World Before Us: How Science is Revealing a New Story of Our Human Origins. Penguin ... The technique was more sophisticated than earlier methods of lithic reduction, involving the striking of lithic flakes from a ...
Les outils agricoles à l'épreuve de l'open source". Techniques & Culture. Revue semestrielle d'anthropologie des techniques (in ...
Decker, Kris De (6 June 2017). "Comment bâtir un internet low tech". Techniques & Culture. Revue semestrielle d'anthropologie ... Different versions of Wi-Fi use different techniques, 802.11b uses DSSS on a single carrier, whereas 802.11a, Wi-Fi 4, 5 and 6 ... Mateti, Prabhaker (2005). "Hacking Techniques in Wireless Networks". Dayton, Ohio: Wright State University Department of ... or 4G networks using the tethering technique. Many smartphones have a built-in capability of this sort, including those based ...
Artistic techniques, Contemporary art movements, DIY culture, Fluxus, Postmodern art, Cassette culture 1970s-1990s). ... Certain materials and techniques are commonly used and frequently favored by mail artists due to their availability, ... Various printmaking techniques, in addition to rubber stamping, are used to create multiples. Copy art (xerography, photocopy) ... Mail artists often use collage techniques to produce original postcards, envelopes and work that may be transformed using copy ...
The technique nishijin-ori, traditionally produced in the Nishijin area of Kyoto, is intricately woven and can have a three ... ISBN 0-88011-598-X. Dalby, Liza (2001). Kimono, Fashioning Culture. Vintage. ISBN 0-09-942899-7. Fält, Olavi K.; Nieminen, Kai ... Bennett, Gary (1997). Aikido techniques & tactics. Human Kinetics Publisher. ... dye technique. Obidome (帯留, "sash clip") is a small, decorative brooch fastened onto the obijime at the front, commonly made ...
Cut-Up Technique , Open Culture". Retrieved 2021-10-31. Marc Lowenthal, translator's introduction to Francis Picabia's I Am a ... Cut-up technique is an extension of collage to words themselves, Tristan Tzara describes this in the Dada Manifesto: TO MAKE A ... Dada Culture (New York and Amsterdam: Rodopi Verlag, 2006) Lavin, Maud. Cut With the Kitchen Knife: The Weimar Photomontages of ... Europe of Cultures. "Tristan Tzara speaks of the Dada Movement" Archived 2015-07-04 at the Wayback Machine, September 6, 1963. ...
Techniques & Culture [Online]. Nugroho, Irawan Djoko (2011). Majapahit Peradaban Maritim. Suluh Nuswantara Bakti. ISBN 978-602- ...
Robert Arthur Andersen (2005). Algal culturing techniques. Elsevier Academic Press. ISBN 9780120884261. Tao, S. H.; Bolger, P. ... For this application, the concentration of germanium dioxide typically used in the culture medium is between 1 and 10 mg/L, ... Germanium dioxide is used in algaculture as an inhibitor of unwanted diatom growth in algal cultures, since contamination with ...
"Historical review of algal culturing techniques". In Anderson, Robert A. (ed.). Algal Culturing Techniques. Elsevier Science & ... Tamiya was able with it to culture algal cell lines that were all in the same developmental stage, a technique used by later ... In 1953 Tamiya, working with other Japanese scientists, developed techniques for the synchronous culture of the green algae ... In 1977 he was given the Japanese Order of Culture for his contributions to science in Japan. The standard author abbreviation ...
ISBN 978-0-02-872100-2. Rubin, Emanuel; Baron, John H. (2006). Music in Jewish History and Culture. Sterling Heights, Michigan ... Boskovich introduced him immediately to twentieth-century techniques and influenced him greatly as a composer and, eventually, ... ISBN 0-674-37471-1. Read, Gardner (1976). Contemporary Instrumental Techniques. New York: Schirmer. ... extended and invented vocal and instrumental techniques, and required virtuosity and perseverance from performers. In fact, ...
1982). Media analysis techniques. Beverly Hills: Sage Publications. (1984). Signs in contemporary culture: An introduction to ... 2011). Ads, Fads, and Consumer Culture: Advertising's Impact on American Character and Society. Boulder, Colo: Rowman & ... Walnut Creek, California: AltaMira Press (1998). Media research techniques. Thousand Oaks: Sage Publications. (2003). Durkheim ... Asa Berger Professor Emeritus Broadcast and Electronic Communication Arts San Francisco State University.The Popular Culture ...
Techniques et culture, 1 (67), Éditions de l'EHESS, 288 p., 2017. Vidal, Denis; Meyer, Eric; Tarabout, Gilles, eds. (1994). ... 2017). "67 , 2017 Low Tech ? Wild Tech !". Techniques & Culture (67). doi:10.4000/tc.8260. Grimaud, Emmanuel; Tastevin, Yann ... 2017). "67 , 2017 Low Tech ? Wild Tech !". Techniques & Culture (67). doi:10.4000/tc.8260. "Persona, étrangement humain , Actes ... Techniques et Culture , 2017 Vidal, Denis (2012). "Vers un Nouveau Pacte Anthropomorphique! Les Enjeux Anthropologiques de la ...
... the technique had rare appearances in Africa forming another facing called opus africanum.: 189-90 The technique used vertical ... Van Oyen, Astrid (2017). "Finding the Material in 'Material Culture'. Form and Matter in Roman Concrete.". In Van Oyen, Astrid ... Along with other facings, the technique is also important for dating buildings in modern scholarship where there is an absence ... The chronology of the usage of facings is fluid with techniques being used long after their popularity has waned.: 252 ...
Coaches use many techniques. Borrowing the concept of modeling from NLP, a weight loss coach will identify the behaviors ... One of the first texts to highlight the relationship between behavior, culture and weight gain. In her book Susie looks at how ...
"Luzhou Spirit Brewing Techniques". China Intangible Cultural Heritage. Ministry of Culture, P.R.China. Archived from the ...
Yarns were also dyed in a wide range of hues, used together in loom weaving and many other techniques. This combination of ... While the Paracas culture developed in this region between approximately 1200 BCE and 100 BCE, the Topará culture is thought to ... Nazca Culture and iconography are believed by scholars such as Helaine Silverman to have evolved from Paracas culture. Hendrik ... The two cultures coexisted for one or more generations, both on the Paracas Peninsula and in the nearby Ica Valley. Their ...
Frankfurt/M.: Suhrkamp, vols.1-2. Mauss, Marcel 1934: "Les techniques du corps." - English 1973: "Techniques of the Body." In: ... "Culture" in singular is an abstraction. The study of body culture is always a study of body cultures in plural. Body cultures ... The study of different class habitus (→class culture), youth cultures, gender cultures (→gender identity) etc. opened up for ... As body culture studies analyse culture and society in terms of human bodily practices, they are sometimes viewed as a form of ...
Cell culture techniques, Microfluidics). ... This technique is relatively cheap and can be used to make ... Young EW, Beebe DJ (March 2010). "Fundamentals of microfluidic cell culture in controlled microenvironments". Chem Soc Rev. 39 ... culture cells as well as perform many other important biological studies. One unique feature that results from miniaturization ... can either lend to the advantages of using microfluidics or it can necessitate further refinement of experimental technique. In ...
Training strategy After study of the current policing system and the culture... a comprehensive training strategy is devised to ... Main elements of the training strategy in NHMP are: • TNA to assess training needs • SWOT Analysis Training Techniques • ... Quality Instructors • Modern Techniques of Instruction 1.Syndicate/Participative System 2.Simulation Exercises 3.Case Studies 4 ...
... after its technique. Until then, most were prepared by using casting techniques, and embossing and fashioning from solid pieces ... Yemeni culture, Jewish culture, Jews and Judaism in Yemen, Jewish Yemeni history, History of Yemen). ... Today, in modern techniques, they use either a thin metal wire which is melted or plate of same substance. The purpose of the ... Granulation was a common form of decorative technique used in the making of rings, bracelets and large necklaces. Granulated ...
Miller, Erica T. (1996). SalonOvations' Day Spa Techniques. Cengage Learning. ISBN 1562532618. Whitney, Nicole. "What Are the ... v t e (Bathing, All stub articles, Culture stubs). ...
Popular Culture and Philosophy. Vol. 57. Open Court Publishing Company. ISBN 9780812697162. Popoff, Martin (2017). Rush: Album ... Technique. Hal Leonard Corporation. 2015. ISBN 9781495032714. Berti, Jim; Bowman, Durrell (2011). Reisch, George A. (ed.). Rush ...
10.2003 on 8 October, National Culture and Arts Foundation 7th National Literary Award. Taiwanese art 1. 家庭美術館 美術家傳記: 陳慧坤 2.蒼松映 ... In order to study Eastern and Western art ideology and techniques, Chen made two research trips to Japan in the 1950s which ... In addition, he also received the Culture Award of Executive Yuan (1997), The Art & Business Awards of the Council for Cultural ... 5.1997 on 29 December, issued by the Executive Yuan, Executive Yuan Culture Award. 6.1999 on 26 April, issued by the Council ...
In economic affairs, they increasingly adapted the methods of the world: taking out loans, using mass marketing techniques. ... dual aspects of Shaker impacts at the close of their way of life at Union Village as both warming to mainstream worldly culture ...
Manuel Ramirez Rosales who inherited much of his technique and his colour schemes as well as a fascination with French culture ... His technique largely resembles the Cuzco School so his portrayal of faces was not particularly accurate, appearing flat and ... The attempt to bring the European model to Chile by replicating its techniques has been seen as a failure in terms of the ... The use of art in this time could be aesthetic, practical, ritual or religious, depending on the culture and the resources ...
Educators such as Freire and Ramón Flecha have also developed a body of theory and techniques for using egalitarian dialogue as ... Una difesa filosofica del confronto pacifico tra culture", Carocci, Roma, 2011 Suitner, Riccarda (2022) The Dialogues of the ... From Dialogical Leadership to a Dialogical Corporate Culture. Heidelberg: Menon 2013. p. 10. Dietz: Acting Independently for ... apart from rational deliberations or culture wars); dramatization to call widespread attention to the issue at hand; and, ...
Cerf said the social culture (group dynamics) that first evolved during the work on the ARPANET was as important as the ... Abbate 2000, p. 38 The NPL group influenced a number of American computer scientists in favor of the new technique, and they ... Simply, they possess the Internet culture, are interested in it and, notably, participate in IETF." Russell notes that Cohen, ... They believed speech traffic would continue to dominate data traffic and believed in traditional telegraphic techniques. Baran ...
On March 27, 2018, Wylie provided evidence to the Digital, Culture, Media and Sport Committee of the UK Parliament that ... In November 2015, Eunoia Technologies pitched Facebook data-mining techniques to the Liberal Party of Canada, securing a $ ... then you have won that culture war." Wylie has said he did not realize the "potential misuse" of his research at the time, ... Written evidence submitted by Aleksandr Kogan to the Digital Culture, Media and Sport Select Committee', House of Commons (UK ...
... applying special techniques of therapeutic physical training. Within the framework of administrative divisions, it is ... health resort in the last third of the 19th and the early 20th centuries attracted famous representatives of Russian culture, ...
The culture of the Assyrian elite was strongly influenced by Babylonia in the south; in a vein similar to how Greek ... and the development of new and innovative siege warfare techniques. At the height of the Neo-Assyrian Empire, the Assyrian army ... Ethnicity and culture are largely based in self-perception and self-designation. A distinct Assyrian identity seems to have ... Though in some aspects influenced by Assyrian culture, these states were for the most part not ruled by Assyrian rulers. Assur ...
The absurdist comedy deals with the politics and hype behind media technology and nerd culture. Grenzfurthner calls his film a ... In their infamous 1972 book Anti-Oedipus, Gilles Deleuze and Félix Guattari have identified the technique of bricolage as the ... France Culture / Place de la toile politique de l'algorithme; May, 2014 IFJ / Phuture Fest Echsenfriedl fan art, monochrom/blog ... Net culture magazine Furtherfield calls it a "hackploitation art house film" that reimagines "the makerspace as grindhouse", a ...
"Explanatory momerandum to the Age Appropriate Design Code". The U.K. Department for Digital, Culture, Media & Sport. 11 June ... the adoption of obfuscation techniques as a way of evading and protesting digital surveillance). Internet activist Eli Pariser ... The neo-colonial structures and discourses are already part of the different "postcolonial" cultures. The postcolonial era in ... Indian Country: Essays on Contemporary Native Culture. Waterloo: Wilfrid Laurier University Press. Alfred, T. 2009. Wasáse: ...
"The Empire of the Great Mughals: History, Art and Culture". Reaktion Books, February 2004. ISBN 1-86189-185-7 Tennent, Emerson ... "used this technique to chastise 'rebels' and then in the end the prisoners, presumably much chastened, were given their lives ... Annemarie Schimmel (2004). The Empire of the Great Mughals: History, Art and Culture. Reaktion Books. p. 96. ISBN 9781861891853 ... Elephants in Indian culture, Execution methods, Social history of India, Social history of Africa, History of South Asia, ...
"Comicvoice: Community education through sequential art." Pop Culture Association - American Culture Association, St. Louis, MO ... or picturevoice which includes techniques such as photo-elicitation and digital storytelling. These techniques allow research ...
It draws heavily on Marxist theory and techniques, and is associated with geographers such as David Harvey and Richard Peet. ... cultures, economies, and their interactions with the environment. It analyzes spatial interdependencies between social ... links to possibilism and cultural ecology some of the same notions of causal effect of the environment on society and culture ... geografie Transactions of the Institute of British Geographers Geography portal AP Human Geography Concepts and Techniques in ...
In the same year, he printed a new currency for New Jersey based on innovative anti-counterfeiting techniques he had devised. ... Olsen, Neil C. (2013). Pursuing Happiness: The Organizational Culture of the Continental Congress. Nonagram Publications. ISBN ... a common decision-making technique, now sometimes called a decisional balance sheet: ... my Way is, to divide half a Sheet of ... Philosophers of culture, Philosophers of education, Philosophers of ethics and morality, Philosophers of history, Philosophers ...
Blaha, who resigned as chairman of Orthopedics at WVU, was quoted as criticizing the institute as being part of a culture at ... There are no steps, no uniformly appropriate internal attitudes, and no techniques within it. The "health of the helper" is ... Health Realization ("HR"), like Sydney Banks's philosophy, is deliberately not taught as a set of "techniques" but as an ... and culture. This level of organizational influence is impressive when the relatively modest level of VVCRP staff time and ...
Martial arts culture, Wrestling culture, Rugby league terminology, Rugby union terminology). ... There are many described techniques for the drainage of blood in the acute stage to prevent hematoma, including simple needle ... This has been achieved with many techniques including: direct pressure dressings, in and out mattress sutures, buttons placed ...
... a lady's man whose favorite pick-up technique is tricking women by vaguely implying he is an international terrorist named " ... "Carlos director Olivier Assayas on the terrorist who became a pop culture icon". The Guardian. London. Archived from the ...
The techniques of responsive evaluation and portrayal were used combined with Context, Input, Process, Product (CIPP) and ... Bitzer was more forthright about CDC's failure, blaming their corporate culture for the problems. He noted that development of ...
... a technique by which the deejay switched from break to break at the height of the party. This technique is specifically called ... Since this emerging culture was still without a name, participants often identified as "b-boys", a usage that included and went ... Grandmaster Flash suggests that Herc may not have kept pace with developments in techniques of cueing (lining up a record to ... him with an education in New York City street culture. He began running with a graffiti crew called the Ex-Vandals, taking the ...
In order to capture the full lexical diversity of meanings for each word, the dictionary employs techniques of synchronic and ... As Germany's Federal President Richard von Weizsäcker once wrote: "The Rechtswörterbuch incorporates language- and culture- ...
Rorty introduces his own ideas into pragmatism which includes the importance of culture, beliefs, and context. He shifts from ... Quantitative research requires skills of data-analysis and several techniques of statistic reasoning, while qualitative ...
It is a technique devised to enrich the way of conveying a poem in a limited space. The general pattern is as follows: Using ... Figurative Elements in Koto and Bunraku Music and Their Analogues in Related Forms of Japanese Culture. Osaka University, Japan ... The provenance of the technique is unclear, however it is likely it was already in common use in the period before writing was ... In English a similar technique is sometimes employed in both poetic and prose language. One contemporary example is: "They say ...
... it presents a theory that science changes due to an accumulation of observations and improvement in experimental techniques and ... Christian culture Role of the Christian Church in civilization The Reformation and its influence on church architecture ... W. Williams, Peter (2016). Religion, Art, and Money: Episcopalians and American Culture from the Civil War to the Great ...
Rindner, Grant (July 18, 2017). "Sheer Mag: Need to Feel Your Love". Spectrum Culture. Retrieved January 23, 2018. Levinson, ... one reviewer comparing this technique favorably to the works of The Hold Steady. However, Levinson was more mixed on the album ...
The technique was developed by Polish physicist Georges Nomarski in 1952. DIC works by separating a polarized light source into ... DIC is used for imaging live and unstained biological samples, such as a smear from a tissue culture or individual water borne ... 1. Unpolarised light enters the microscope and is polarised at 45°. Polarised light is required for the technique to work. 2. ... Its resolution[specify] and clarity in conditions such as this are unrivaled among standard optical microscopy techniques. One ...
Criticism for the violent aspects of video game culture has come from a number of anti-violence groups, and perhaps the most ... Advertisement placement and other marketing techniques have in the past targeted women as more receptive to non-violent video ...
New diagnostic techniques, such as trichoscopy may be used for non-invasive differential diagnosis of cicatricial alopecia. ... In addition, if pustules are present, cultures are taken to identify which microbes, if any, may be contributing to the ...
The same technique is used in German-speaking countries to express approval at meetings. In Jamaica people may bang lids and ... In Deaf culture, Deaf audiences will use a more visually expressive variant of clapping. Instead of clapping their palms ... The age of the custom of applauding is uncertain, but it is widespread among human cultures. The variety of its forms is ... However, in a situation more specific to hearing culture, the traditional clap is used. In German-speaking countries, it is ...
The technique has four main parts: A field-worthy notebook where one records direct observations as they are being observed. A ... Additionally, in social science research that involves studying culture, it is important to faithfully capture language and ... The Grinnell technique has been regarded by many ornithologists as one of the best standardized methods for taking accurate ... Develop one's own transcribing language: While no one technique of transcribing (or "jotting") is perfect, most qualitative ...
Base Mérimée: Search for heritage in the commune, Ministère français de la Culture. (in French) v t e (Articles with French- ... Amenities: Pharmacy, Doctor, Mairie, small Post Office, carwash, Automat laundry, Technique Controle - repair garage, ...
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Water Sampling Strategies and Culture Techniques for Detecting Legionellae. Legionella spp. are ubiquitous and can be isolated ... When culturing faucet aerators and shower heads, swabs of surface areas should be collected first; water samples are collected ...
... 0-9. A. B. C. D. E. F. G. H. I. J. K. L. M. N. O. P. Q. R ... Use of cell culture in virology for developing countries in the South-East Asia Region  ...
Newer Diagnostic Techniques. Nucleic acid amplification tests such as polymerase chain reaction (PCR) and transcription- ... Culture and Histology. Bacterial cultures on blood and chocolate agar are indicated in every case of neonatal conjunctivitis ... Culture of corneal epithelial cells for HSV if cornea is involved; PCR should also be considered in cases of possible HSV ... HSV infections may be more rapidly diagnosed with PCR, and PCR testing for HSV is more sensitive than viral culture. [11] ...
Workshop on Cell Culture Techniques and Internal Quality Control, Hong Kong, 1-6 December 2003 : report  ... Hands-on Training Workshop on Cell Culture Techniques for the Laboratory Diagnosis of Polio/Enteroviruses and Measles/Rubella ... A simple aseptic method for the rapid removal of samples from small-scale cultures of micro-organisms / by S. G. Williams, W. H ... Use of cell culture in virology for developing countries in the South-East Asia Region  ...
... .social-ris-container { display: flex; ... Culture Change is the Key to Success. The SLUG Bug collaborative was one of the first to utilize orchestrated testing to ... But it took more than procedures and techniques to reduce CLASBI rates. It took teamwork, collaboration and what I believe is ... But what we quickly found out was that other methods of central line care, include tubing change technique, hub care monitoring ...
Support Open Culture. Were hoping to rely on our loyal readers rather than erratic ads. To support Open Cultures educational ... Open Culture was founded by Dan Colman. We use cookies on our website to give you the most relevant experience by remembering ... Based in Seoul, Colin Marshall writes and broadcasts on cities and culture. Hes at work on a book about Los Angeles, A Los ... Richard Feynmans "Notebook Technique" Will Help You Learn Any Subject-at School, at Work, or in Life. in Education, ...
We want you to be nothing but successful here at Cultures for Health. Have Questions? Check out these resources to get you the ... Wherever hes gone, he has gleaned valuable insights into the cultures and traditions of local and indigenous peoples, whether ... Sandor Katzs Fermentation Journeys: Recipes, Techniques, and Traditions from around the World. ... Sandor Katzs Fermentation Journeys: Recipes, Techniques, and Traditions from around the World ...
Pablo Trejo/Ministry of Culture of Columbia, 2019 The traditional knowledge and techniques associated with Pasto Varnish mopa- ... Carlos René Quintero/Ministry of Culture of Colombia, 2019. © Carlos René Quintero/Ministry of Culture of Colombia, 2019. © ... Carlos René Quintero/Ministry of Culture of Colombia, 2019. © Carlos René Quintero/Ministry of Culture of Colombia, 2019. © ... Carlos René Quintero/Ministry of Culture of Colombia, 2019. © Carlos René Quintero/Ministry of Culture of Colombia, 2019. © ...
Phrom Lok: Where traditional southern Thai culture meets modern-day farming techniques. September 13, 2018. September 15, 2018 ...
Understanding Culture Appropriation: Explanations and Techniques to Avoid It. *. * by Grady Capstone Journalist December 28, ... Why Its Newsworthy: Its an issue that can affect minority cultures whose culture they believe is being misappropriated. ... As holidays pass year after year, a practice now known as culture appropriation happens as well. ...
A new method of culturing the olfactory epithelium was devised. This slice culture technique improved the success rate for ... Four culture techniques were performed, including whole explants and explanted .... View more ,Objective To improve the success ... Four culture techniques were performed, including whole explants and explanted biopsy slices. Five days after plating, neuronal ... The techniques presented here make the human olfactory epithelium a useful model for clinical research into certain olfactory ...
Culture générale. *Content Security Policy sur Wikipédia. Connaissances techniques. *Documentation Content Security Policy sur ...
"Technique 22: Presentation of Self, Material Culture, Poverty". TRAILS: Teaching Resources and Innovations Library for ... Erin K Anderson, A Quest for Culture in the Campus Community , TRAILS: Teaching Resources and Innovations Library for Sociology ... Keith D. Wilkins, Culture Through Everyday Objects , TRAILS: Teaching Resources and Innovations Library for Sociology: All ... Paul D. Starr, Technique 11: Historical Time: Modern Industrial Society in PerspectiveTeaching Objectives/Student Learning ...
"Tissue Culture Techniques" by people in this website by year, and whether "Tissue Culture Techniques" was a major or minor ... A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured ... "Tissue Culture Techniques" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Tissue Culture Techniques" by people in Profiles. ...
Title : Fluroescent antibody technique for identification of presumptively positive gonococcal cultures. Personal Author(s) : ... Bacteriological Techniques Fluorescent Antibody Technique Indicators And Reagents Methods Neisseria Gonorrhoeae Research ... Direct FA technique using Flazo orange counterstain in identification of Neisseria gonorrhoeae Cite ... Title : Direct FA technique using Flazo orange counterstain in identification of Neisseria gonorrhoeae Personal Author(s) : ...
... Das Seminar für Medienwissenschaft der Universität Basel veröffentlicht seit 2020 ... Die Basel Media Culture and Cultural Techniques (BMMCT) Working Papers erscheinen in unregelmässigen Abständen in deutscher und ... Potent Mercantile Metaphors of the Programming Language JAVA (Basel Media Culture and Cultural Techniques Working Papers 3). ... Seeing Aesthetically and Scientifically (Basel Media Culture and Cultural Techniques Working Papers 5). Hannes Bajohr (2020): ...
The course is given by Methodology and Techniques of Archaeological Research (S) (2004/2005) - Masters in Art History (until ... Dep.Cultures and Civilizations,University of Verona ... Methodology and Techniques of Archaeological Research (S) (2004 ...
A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. In: Cell ... A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. Cell ... A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. / Dorko ... A new technique for isolating and culturing human hepatocytes from whole or split livers not used for transplantation. ...
Inspiration from the Worlds Great Traditions Join Melanie Marquis as she explores an amazing assortment of magickal techniques ... A Witchs World of Magick: Expanding Your Practice with Techniques & Traditions from Diverse Cultures - by Melanie Marquis ... Home / A Witchs World of Magick: Expanding Your Practice with Techniques & Traditions from Diverse Cultures - by Melanie ... and an abundance of other techniques. With these new perspectives on the common threads that weave throughout our magickal ...
A new technique that can analyse how drug molecules bind to proteins in tissue samples could offer an improved route to drug ... Cell cultures enable the effects of different compounds to be tested on specific biological targets involved in various ... Action of drug compounds in tissue revealed by new technique. A new technique that can analyse how drug molecules bind to ... This new technique, described in a paper published today (14 July 2022) in Angewandte Chemie, enables researchers to use real ...
Rhetorical Techniques Used In Supersize Me. 705 Words , 3 Pages. *. Eric Schlosser And Charles Wilsons Chew On This. 594 Words ... Schlossers Fast Food Culture. 328 Words , 2 Pages. Fast food nation spilled many incriminating facts about Americas fast food ... Rhetorical Techniques Used In Supersize Me. 705 Words , 3 Pages. In 2003, a brave, middle-aged man named Morgan Spurlock ... Eminent voices in the food culture convince people that McDonalds is unhealthy. Most processed foods are considered unhealthy ...
Tip Culture Is Getting Insane: Starbucks Customers Furious Over Companys New Tipping System ...
The cell culture is grown in a "light" medium and a "heavy" medium. These mediums are given identical conditions except for one ... 7.1 Labelling Techniques *7.1.1 Isotope-Coded Affinity Tag (ICAT). *7.1.2 Stable Isotope Labeling with Amino Acids in Cell ... This technique is often coupled with mass spectrometry for protein analysis. History of ESI[edit , edit source]. The research ... Labelling Techniques[edit , edit source]. Before peptide tagging was used to study protein dynamics, quantitative proteomics ...
Home/Culture and Society/Science and Technology/DVD Review: Guitar DVD - Total Scales Techniques And Applications By Mark John ... Lesson 9, More Techniques here continues additional techniques such as pull offs, hammer pull/pull hammer, hammer pull with ... Lesson 12, Tremolo/Whammy Bar Techniques describes the technique of using the whammy bar that is available on certain ... DVD Review: Guitar DVD - Total Scales Techniques And Applications By Mark John Sternal. T. Michael Testi January 9, 2008 1 ...
Technique & culture. The journal Techniques & culture is devoted to the pragmatic, social and symbolic dimensions of techniques ... Le carnet de Techniques & Culture est le blog de la revue danthropologie des techniques. Vous pourrez suivre les débats et ... Conference: "Les Rencontres Techniques&Culture", Marseille, January 2021. An androcentric history of technologies. The history ... This issue of Techniques&Culture aims to elaborate on this program-which still remains to be fully explored-through case ...
... cell culture tested; Suitable for mammalian cell culture; ... technique(s). cell culture , mammalian: suitable. impurezas. , ... recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture. ...
La loi … - Selection from Pratique des techniques bancaires [Book] ... Culture générale. Une première version de la loi Scrivener est entrée en vigueur le 10 janvier 1978 ; elle a été ensuite ... Pratique des techniques bancaires by Aurélien Giraud. Get full access to Pratique des techniques bancaires and 60K+ other ... Get Pratique des techniques bancaires now with the OReilly learning platform.. OReilly members experience live online ...
... whose goal is to create a better culture."[22] Charles H. Lippy writes that earlier spiritual interest in the technique faded ... The Transcendental Meditation technique (abbreviated as TM) is the technique associated with the practice of Transcendental ... Transcendental Meditation (TM), a concentrative technique ... has been the most extensively studied meditation technique.. ... For example, TM is simply - as they state - a technique. Although it enables one to cope with life, it offers no goal beyond ...
  • Since Chlamydia bacteria are obligate intracellular organisms, the culture specimens need to contain epithelial cells and not just exudative material. (medscape.com)
  • Anderson DG, Markova D, An HS, Chee A, Enomoto-Iwamoto M, Markov V, Saitta B, Shi P, Gupta C, Zhang Y. Human umbilical cord blood-derived mesenchymal stem cells in the cultured rabbit intervertebral disc: a novel cell source for disc repair. (rush.edu)
  • Part of the early stages of drug discovery takes place in cell cultures, clusters of cells that are grown in the laboratory, outside of their natural environment. (birmingham.ac.uk)
  • Requirements: Students should have completed at least one biology course and have an interest in learning common techniques for culture of animal cells. (missouriwestern.edu)
  • The overall aims of this project are to study the effects of various probiotic bacteria or prebiotics on intestinal inflammation, using cultured mammalian cells and/or mice as experimental models. (missouriwestern.edu)
  • Antigens are peptides or recombinant or native dependent on the production method.For cells, cell lines and tissues in culture till half confluency. (labograde.com)
  • An in vitro BBB model with a co-culture of rat brain microvascular endothelial cells (RBECs) and microglia cells using Transwell chambers was established to assess the RSV delivery across BBB. (nih.gov)
  • Cell culture is the process of taking cells out of the body and growing them in an artificial environment. (grstiftung.ch)
  • To date, standard cell culture comprises that cells live in a soup of nutrients on the surface of relatively simple plastic dishes. (grstiftung.ch)
  • We have designed novel biomimetic cell culture surfaces that imitate the softness of various tissues and organs, allowing the cells to «feel like home» even outside the body. (grstiftung.ch)
  • A second aspect of the project consists in quantifying the benefits of culturing cells on our innovative cell culture system. (grstiftung.ch)
  • The coating does not interfere with conventional biological techniques to retrieve cells for biochemical or molecular analysis. (grstiftung.ch)
  • Further experiments have been carried out to characterize the behavior of cells grown on our innovative culture substrates. (grstiftung.ch)
  • Cytology is used mainly for diagnosing infant inclusion conjunctivitis and ocular trachoma through the demonstration of intracytoplasmic C trachomatis inclusions in HeLa cells (ie, continuously cultured carcinoma cell line used for tissue cultures). (medscape.com)
  • C trachomatis grows well in a variety of cell lines (eg, McCoy and HeLa cells) that can be maintained in tissue culture. (medscape.com)
  • This new technique, described in a paper published today (14 July 2022) in Angewandte Chemie, enables researchers to use real tissue samples to assess which proteins the drug will bind to in the body and therefore how effective it is likely to be against the target. (birmingham.ac.uk)
  • Tissue Culture Techniques" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (rush.edu)
  • Nodal explants were cultured on MS medium containing various concentrations of KIN (0, 0.5, 1 and 1.5mg.l-1) in combination with IBA (0, 0.025 and 0.5mg.l-1). (who.int)
  • This technique allowed for the isolation of human hepatocytes on a large scale (up to 18.5 × 10 9 in one case) from normal and diseased liver specimens. (utmb.edu)
  • We conclude that the technique presented here allows isolation of large numbers of human hepatocytes from specimens unsuitable for transplantation but very valuable for biomedical research. (utmb.edu)
  • Conclusive diagnosis of these viruses requires electron microscopic examination of stool specimens, a laboratory technique that is available only at a few large centers, including CDC. (cdc.gov)
  • Suspected and confirmed SARS-CoV-2 positive clinical specimens, cultures, or isolates should be packed and shipped as UN 3373 Biological Substance, Category B. (cdc.gov)
  • Cell culture is also preferred for rectal specimens because nonculture test results are difficult to interpret in the presence of stool organisms. (medscape.com)
  • Next steps for the research will include improving the sensitivity of the technique, and extending it to other types of drug compounds. (birmingham.ac.uk)
  • Comparison of both culture -independent techniques can provide a general overview of the whole process, although DGGE does not reveal the diversity expected due to the reported problems with the sensitivity of this technique . (bvsalud.org)
  • Because of its high specificity (100%) and sensitivity, cell culture is the only test that should be used to establish the presence or absence of infections in cases with legal implications, such as those involving rape or sexual abuse. (medscape.com)
  • Students will learn basic tissue culture techniques, including aseptic techniques, maintenance and propagation of cultured cell lines, and assays for analysis of cellular functions. (missouriwestern.edu)
  • Experience in aseptic technique, cell culture, and retroviral or lentiviral transductions. (nih.gov)
  • A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. (rush.edu)
  • Large numbers of human hepatocytes were obtained from split and whole livers by using an adaptive form of the collagenase perfusion technique employed in rodent and human biopsies. (utmb.edu)
  • The presence of HSV in tissue culture remains the criterion standard in the diagnosis of HSV, despite a high false-negative rate. (medscape.com)
  • To compare the performance of the Ogawa-Kudoh method with the modified Petroff technique in diagnosis of pulmonary tuberculosis. (scielo.br)
  • The Ogawa-Kudoh technique proved to be sufficiently sensitive and specific for diagnosis of pulmonary tuberculosis, and, therefore, suitable for routine laboratory application. (scielo.br)
  • But what we quickly found out was that other methods of central line care, include tubing change technique, hub care monitoring, CVC access limitation and CVC removal monitoring, varied widely in local hospital practice. (infectioncontroltoday.com)
  • Feynman had to go face-to-face with the elders of the Princeton physics department, but if you don't benefit from that kind of institutional threat, you might consider putting into practice another Feynman technique: "teaching" what you've learned to someone else. (openculture.com)
  • As holidays pass year after year, a practice now known as culture appropriation happens as well. (uga.edu)
  • Providing instructions on how to unite classic beliefs with modern practice, A Witch's World of Magick uncovers the universal principles that underlie decoy magick, curse breaking, potion making, number magick, and an abundance of other techniques. (hexwitch.com)
  • The Transcendental Meditation technique (abbreviated as TM ) is the technique associated with the practice of Transcendental Meditation developed by the Indian spiritual figure Maharishi Mahesh Yogi . (wikipedia.org)
  • The author praises the congress initiative of highlighting the metapsychological writings as a place for dialogues with practice, technique and culture. (bvsalud.org)
  • A bronchoscopic culture is done to find infection in the lung that cannot be accurately detected by a sputum culture . (medlineplus.gov)
  • No organisms are seen on the culture. (medlineplus.gov)
  • Not all organisms found with bronchoscopic culture need to be treated. (medlineplus.gov)
  • Approximately 30% of the organisms contained in the microbiome have been cultured, but not all organisms can be grown in vitro with current techniques. (medscape.com)
  • This graph shows the total number of publications written about "Tissue Culture Techniques" by people in this website by year, and whether "Tissue Culture Techniques" was a major or minor topic of these publications. (rush.edu)
  • Below are the most recent publications written about "Tissue Culture Techniques" by people in Profiles. (rush.edu)
  • Until the 1970s, diagnostic techniques for infectious diarrhea were limited to bacteria and protozoa, and an etiologic agent could be identified in a limited proportion of cases. (cdc.gov)
  • Bronchoscopic culture is a laboratory exam to check a piece of tissue or fluid from the lungs for infection-causing germs. (medlineplus.gov)
  • Any laboratory that cultures Legionella from environmental samples is encouraged to enroll. (cdc.gov)
  • Does the laboratory routinely perform culture for Legionella ? (cdc.gov)
  • If you need samples tested during an investigation into cases of disease, you should consider using a laboratory that has documentation from a proficiency testing program like the Environmental Legionella Isolation Techniques Evaluation Program. (cdc.gov)
  • Cultures of SARS-CoV-2 should be handled in a Biosafety Level 3 (BSL-3) laboratory using BSL-3 practices, and inoculation of animals with infectious wild-type SARS-CoV-2 should be conducted in an Animal Biosafety Level 3 (ABSL-3) facility using ABSL-3 practices and respiratory protection. (cdc.gov)
  • Mass spectrometry is an analytical technique used for identifying the mass of a compound based on the mass-to-charge ratio of charged particles. (wikibooks.org)
  • Bacterial cultures on blood and chocolate agar are indicated in every case of neonatal conjunctivitis and remain the criterion standard despite newer diagnostic methods. (medscape.com)
  • Here he also shows methods of holding a pick while tapping as well as how this technique can be just as effective on acoustic as it is on electric. (blogcritics.org)
  • The Ogawa-Kudoh and modified Petroff methods were efficient in mycobacteria detection, with no significant differences in results (p=0.549) and contamination rate of the cultures (p=0.065). (scielo.br)
  • Our results are in agreement with those obtained with culture independent methods , but qPCR showed higher precision and a different behavior was observed for each yeast species and at each temperature assayed. (bvsalud.org)
  • Majors must complete three required courses (listed below), one Area Focus and Regional Perspectives on Culture course, one anthropological methods course, one anthropological theory course and 15 credits of electives based on student interest. (syr.edu)
  • Students in the major select at least one methods course, such as Methods in Archaeology, Ethnographic Techniques or Life Histories/Narratives. (syr.edu)
  • Studies on embryo toxic effects of thallium using the whole embryo culture technique. (cdc.gov)
  • Lesson 12, 'Tremolo/Whammy Bar Techniques' describes the technique of using the whammy bar that is available on certain electric guitar models. (blogcritics.org)
  • One canvasser describes the technique in rural areas as a 'social call' as in many rural locations people tend to know their local TD. (rte.ie)
  • The organism can be grown in culture using a specific liquid medium developed by Kelly. (medscape.com)
  • What has changed in recent years, however, is the advent of the technique known as CRISPR. (iflscience.com)
  • Not required, but would be of great benefit: knowledge of iPSC culture, CRISPR design, and/or experience with bioinformatic analysis of next-generation sequencing data. (vanderbilt.edu)
  • In infants with suspected chlamydial conjunctivitis, perform an antigen/DNA detection test, chlamydial culture, or both, using scrapings from the palpebral conjunctiva. (medscape.com)
  • Each biopsy specimen was bisected, and 1 piece was processed for immunohistochemistry or electron microscopy while the other piece was dissected further for explant culture. (edu.au)
  • At the same time, through photography, painting, sculpture and mixed media they celebrate the ongoing presence of Native American art and culture and remind us that art is an essential form of activism. (portlandartmuseum.org)
  • Supported in part by Native Arts & Cultures Foundation and the Museum's Native American Art Council. (portlandartmuseum.org)
  • Productive soil and outstanding growing environments, together with cultured farming techniques and unconventional agricultural tools, are responsible for the optimal environments in productive pastoral, forestry, and cultivation activities. (worldatlas.com)
  • The techniques presented here make the human olfactory epithelium a useful model for clinical research into certain olfactory dysfunctions and a model for the causes of neurodevelopmental and neurodegenerative diseases. (edu.au)
  • EAC organoid cultures provide a pre-clinical tool for studies of clonal evolution and precision therapeutics. (nature.com)
  • In addition, they are unsuitable for large numbers of patients (eg, in the emergency department [ED]). Nevertheless, in certain clinical situations, cultures are mandatory. (medscape.com)
  • Objective To improve the success of culturing olfactory neurons from human nasal mucosa by investigating the intranasal distribution of the olfactory epithelium and devising new techniques for growing human olfactory epithelium in vitro. (edu.au)
  • Cajal improved the techniques of Golgi, which involved staining neurons for observation. (giantmicrobes.com)
  • A design matrix of eight distinct factor/level combinations (test groups) were developed by using four factors: sterile versus clean tubing change technique, hub care compliance monitoring versus not monitoring, CVC line entry access limitation versus not monitoring and CVC line removal tracking versus no tracking policy. (infectioncontroltoday.com)
  • In addition, four health care organizations elected to implement the sterile tubing change technique after reviewing the results of the testing, which resulted in a 64 percent decrease in CLABSI rates from the baseline. (infectioncontroltoday.com)
  • One of the approaches to managing this important pest is use of a sterile insect technique (SIT). (usda.gov)
  • Sterile Insect Technique programs have been developed for management of several tephritid fruit fly pests. (usda.gov)
  • Fluroescent antibody technique for identification of presumptively positive gonococcal cultures. (cdc.gov)
  • The antibody is suitable for the techniques: ICC / IF and ELISA. (who.int)
  • In infants with suspected chlamydial pneumonia, perform a nasopharyngeal swab for Chlamydia culture. (medscape.com)
  • Wherever he's gone, he has gleaned valuable insights into the cultures and traditions of local and indigenous peoples, whether they make familiar ferments like sauerkraut or less common preparations like natto and koji. (culturesforhealth.com)
  • Magickal Inspiration from the World's Great Traditions Join Melanie Marquis as she explores an amazing assortment of magickal techniques gathered from the annals of world folk magick. (hexwitch.com)
  • In 1978, the same technique was used to detect rotavirus, the most common cause of severe diarrhea in children. (cdc.gov)
  • In this excerpt from the programme, canvassers describe their techniques of door-to-door canvassing. (rte.ie)
  • Military officials refused to describe what techniques Peterson had objected to and said all records of them had been destroyed. (democracynow.org)
  • Culture-dependent and independent techniques to monitor yeast species during cold soak carried out at different temperatures in winemaking. (bvsalud.org)
  • Scott teaches non-violent communication techniques that help managers live healthier productive lives. (udemy.com)
  • Lesson 18, 'Exotic Scales' explore the scales of other cultures from around the world. (blogcritics.org)
  • Whether it's silence and solitude you're seeking or an itinerary of action-packed activities, we cover the best places to explore and appreciate Sámi communities and culture in Norway. (lonelyplanet.com)
  • Ailey's style blended genre-including modern, ballet, jazz, and techniques of various African cultures-to explore the plight of the marginalized both onstage and off. (playbill.com)
  • When I was opening Aska,' says chef Fredrik Berselius, 'the idea was to look at New York and its surroundings, then explore it through Scandinavian eyes and food… The food I serve aims to take the guest on a journey of Scandinavian flavours, memories, feelings, and techniques with an emphasis on ingredients from the Northeastern US. (quintessentially.com)
  • 2. The manufacturing employs traditional techniques or processes. (kyoto.jp)
  • Gažiová employs the technique of graffiti and templates on natural canvas. (prague-art.cz)
  • The Environmental Legionella Isolation Techniques Evaluation Program issues documentation of proficiency to laboratories that have demonstrated the ability to isolate Legionella from simulated environmental samples by culture. (cdc.gov)
  • What is the value to using a lab that has documentation of proficiency from the Environmental Legionella Isolation Techniques Evaluation Program? (cdc.gov)
  • Lesson 10, 'Tapping' explores the technique of hammering or tapping a fret and pulling off to an open or fretted note. (blogcritics.org)
  • That long ago one can't determine specific ethnic traits, but we know traits of Sámi culture appear from approximately 2000 years ago, and traces of burial sites and settlement continue right up to today's Sámi population. (lonelyplanet.com)
  • Most importantly, using testis explanted at 16.5 or 18.5 dpc and cultured for 2-4 days, we demonstrated that the kinetics of changes in H3K4me2, H3K4me3, global DNA methylation and on parental imprints can generally be reproduced ex vivo with the model of organ culture without the addition of serum. (nih.gov)
  • One recent improvement for mass rearing oriental fruit flies involves adding wheat germ oil (WGO) to the larval culture medium which improved several parameters of biological performance. (usda.gov)
  • Culture techniques: Applicability for studies on prenatal differentiation and toxicity: 5th symposium on prenatal development, May, 1981, Berlin. (cdc.gov)
  • Preservation techniques and incorporating fermented ingredients have also long been a tradition in Scandinavian food culture. (quintessentially.com)
  • Conclusions This study explains and overcomes most of the variability in the success in observing neurogenesis in cultures of adult human olfactory epithelium. (edu.au)
  • We considered the issue of whether WGO supplementation of the larval culture medium influences the fatty acid make up of adult tissues. (usda.gov)
  • Total Scales Techniques And Applications may be one of the fastest ways to learn how to play guitar. (blogcritics.org)
  • Guitar DVD: Total Scales Techniques And Applications is a DVD training system that is geared for a beginner to intermediate guitar player who is just starting out to learn how to play guitar, as well as for the experienced guitarist who is self-taught and has had little, if any, formal instruction. (blogcritics.org)
  • You will also learn picking techniques and how to work with a metronome. (blogcritics.org)
  • Many of these techniques are really more geared for an electric guitar, but with some experience and adeptness, one can also learn to make this work for an acoustic guitar as well. (blogcritics.org)
  • Here you will learn how to apply many of the techniques to the pentatonic scales. (blogcritics.org)
  • Learn what features have made the most difference to the salon's culture, business, and successful operation. (nailsmag.com)
  • There are myriad Sami-led tour companies in the region to learn more and experience some of their culture. (lonelyplanet.com)
  • Students learn how to show respect in Filipino culture, they learn techniques using sticks and they wear handkerchiefs instead of belts to show their ranking. (wbaltv.com)
  • [ 52 ] In this technique, a couple of drops of blood are added to the medium, which is incubated at 30-37°C and kept for 2 to 6 weeks. (medscape.com)
  • Many hospitals across the country already have evidence-based practices in place to help improve central line infection rates, which include hand hygiene, central line insertion techniques, dressing changes and root cause analysis. (infectioncontroltoday.com)
  • Abnormal culture results usually indicate a respiratory infection. (medlineplus.gov)
  • infection diagnosed by culture. (cdc.gov)