Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.
The beta subunit of human CHORIONIC GONADOTROPIN. Its structure is similar to the beta subunit of LUTEINIZING HORMONE, except for the additional 30 amino acids at the carboxy end with the associated carbohydrate residues. HCG-beta is used as a diagnostic marker for early detection of pregnancy, spontaneous abortion (ABORTION, SPONTANEOUS); ECTOPIC PREGNANCY; HYDATIDIFORM MOLE; CHORIOCARCINOMA; or DOWN SYNDROME.
The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.
A product of the PLACENTA, and DECIDUA, secreted into the maternal circulation during PREGNANCY. It has been identified as an IGF binding protein (IGFBP)-4 protease that proteolyzes IGFBP-4 and thus increases IGF bioavailability. It is found also in human FIBROBLASTS, ovarian FOLLICULAR FLUID, and GRANULOSA CELLS. The enzyme is a heterotetramer of about 500-kDa.
Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.
CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.
A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.
FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form.
A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Blood proteins that bind to THYROID HORMONES such as THYROXINE and transport them throughout the circulatory system.
A prenatal ultrasonography measurement of the soft tissue behind the fetal neck. Either the translucent area below the skin in the back of the fetal neck (nuchal translucency) or the distance between occipital bone to the outer skin line (nuchal fold) is measured.
Blood tests used to evaluate the functioning of the thyroid gland.
A syndrome that results from abnormally low secretion of THYROID HORMONES from the THYROID GLAND, leading to a decrease in BASAL METABOLIC RATE. In its most severe form, there is accumulation of MUCOPOLYSACCHARIDES in the SKIN and EDEMA, known as MYXEDEMA.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Elements of limited time intervals, contributing to particular results or situations.
The beginning third of a human PREGNANCY, from the first day of the last normal menstrual period (MENSTRUATION) through the completion of 14 weeks (98 days) of gestation.
A group of related diseases characterized by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin.
One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
A chromosome disorder associated either with an extra chromosome 21 or an effective trisomy for chromosome 21. Clinical manifestations include hypotonia, short stature, brachycephaly, upslanting palpebral fissures, epicanthus, Brushfield spots on the iris, protruding tongue, small ears, short, broad hands, fifth finger clinodactyly, Simian crease, and moderate to severe INTELLECTUAL DISABILITY. Cardiac and gastrointestinal malformations, a marked increase in the incidence of LEUKEMIA, and the early onset of ALZHEIMER DISEASE are also associated with this condition. Pathologic features include the development of NEUROFIBRILLARY TANGLES in neurons and the deposition of AMYLOID BETA-PROTEIN, similar to the pathology of ALZHEIMER DISEASE. (Menkes, Textbook of Child Neurology, 5th ed, p213)
Natural hormones secreted by the THYROID GLAND, such as THYROXINE, and their synthetic analogs.
A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.
A condition in which total serum protein level is below the normal range. Hypoproteinemia can be caused by protein malabsorption in the gastrointestinal tract, EDEMA, or PROTEINURIA.
Electrophoresis applied to BLOOD PROTEINS.
Abnormally elevated THYROXINE level in the BLOOD.
A plant genus of the family RANUNCULACEAE that contains alpha-hederin, a triterpene saponin in the seeds, and is the source of black seed oil.
A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
Conditions of abnormal THYROID HORMONES release in patients with apparently normal THYROID GLAND during severe systemic illness, physical TRAUMA, and psychiatric disturbances. It can be caused by the loss of endogenous hypothalamic input or by exogenous drug effects. The most common abnormality results in low T3 THYROID HORMONE with progressive decrease in THYROXINE; (T4) and TSH. Elevated T4 with normal T3 may be seen in diseases in which THYROXINE-BINDING GLOBULIN synthesis and release are increased.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Hypersecretion of THYROID HORMONES from the THYROID GLAND. Elevated levels of thyroid hormones increase BASAL METABOLIC RATE.
Inflammation of the MIDDLE EAR including the AUDITORY OSSICLES and the EUSTACHIAN TUBE.
Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.
A hypermetabolic syndrome caused by excess THYROID HORMONES which may come from endogenous or exogenous sources. The endogenous source of hormone may be thyroid HYPERPLASIA; THYROID NEOPLASMS; or hormone-producing extrathyroidal tissue. Thyrotoxicosis is characterized by NERVOUSNESS; TACHYCARDIA; FATIGUE; WEIGHT LOSS; heat intolerance; and excessive SWEATING.
Methods for maintaining or growing CELLS in vitro.
Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A condition characterized by a dry, waxy type of swelling (EDEMA) with abnormal deposits of MUCOPOLYSACCHARIDES in the SKIN and other tissues. It is caused by a deficiency of THYROID HORMONES. The skin becomes puffy around the eyes and on the cheeks. The face is dull and expressionless with thickened nose and lips.
Pathological processes involving the THYROID GLAND.
A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC
Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
Determination of the nature of a pathological condition or disease in the postimplantation EMBRYO; FETUS; or pregnant female before birth.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
Agents that are used to treat hyperthyroidism by reducing the excessive production of thyroid hormones.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
The relationship between the dose of an administered drug and the response of the organism to the drug.
A process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A group of sporadic, familial and/or inherited, degenerative, and infectious disease processes, linked by the common theme of abnormal protein folding and deposition of AMYLOID. As the amyloid deposits enlarge they displace normal tissue structures, causing disruption of function. Various signs and symptoms depend on the location and size of the deposits.
Spontaneously remitting inflammatory condition of the THYROID GLAND, characterized by FEVER; MUSCLE WEAKNESS; SORE THROAT; severe thyroid PAIN; and an enlarged damaged gland containing GIANT CELLS. The disease frequently follows a viral infection.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
Glucose in blood.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A thioureylene antithyroid agent that inhibits the formation of thyroid hormones by interfering with the incorporation of iodine into tyrosyl residues of thyroglobulin. This is done by interfering with the oxidation of iodide ion and iodotyrosyl groups through inhibition of the peroxidase enzyme.
A tripeptide that stimulates the release of THYROTROPIN and PROLACTIN. It is synthesized by the neurons in the PARAVENTRICULAR NUCLEUS of the HYPOTHALAMUS. After being released into the pituitary portal circulation, TRH (was called TRF) stimulates the release of TSH and PRL from the ANTERIOR PITUITARY GLAND.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The rate dynamics in chemical or physical systems.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
A common form of hyperthyroidism with a diffuse hyperplastic GOITER. It is an autoimmune disorder that produces antibodies against the THYROID STIMULATING HORMONE RECEPTOR. These autoantibodies activate the TSH receptor, thereby stimulating the THYROID GLAND and hypersecretion of THYROID HORMONES. These autoantibodies can also affect the eyes (GRAVES OPHTHALMOPATHY) and the skin (Graves dermopathy).
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Pathophysiological conditions of the FETUS in the UTERUS. Some fetal diseases may be treated with FETAL THERAPIES.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Established cell cultures that have the potential to propagate indefinitely.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
The age of the mother in PREGNANCY.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.
Techniques used in studying bacteria.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.
Backflow of blood from the RIGHT VENTRICLE into the RIGHT ATRIUM due to imperfect closure of the TRICUSPID VALVE.
Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Proteins prepared by recombinant DNA technology.
The part of a human or animal body connecting the HEAD to the rest of the body.
The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sum of the weight of all the atoms in a molecule.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
A constituent of STRIATED MUSCLE and LIVER. It is an amino acid derivative and an essential cofactor for fatty acid metabolism.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A 191-amino acid polypeptide hormone secreted by the human adenohypophysis (PITUITARY GLAND, ANTERIOR), also known as GH or somatotropin. Synthetic growth hormone, termed somatropin, has replaced the natural form in therapeutic usage such as treatment of dwarfism in children with growth hormone deficiency.
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Abstaining from all food.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.
The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated as the time from the last day of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization.
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Proteins found in any species of bacterium.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Physiological processes and properties of the BLOOD.
Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
A test to determine the ability of an individual to maintain HOMEOSTASIS of BLOOD GLUCOSE. It includes measuring blood glucose levels in a fasting state, and at prescribed intervals before and after oral glucose intake (75 or 100 g) or intravenous infusion (0.5 g/kg).
Diminished effectiveness of INSULIN in lowering blood sugar levels: requiring the use of 200 units or more of insulin per day to prevent HYPERGLYCEMIA or KETOSIS.
All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
The means of interchanging or transmitting and receiving information. Historically the media were written: books, journals, newspapers, and other publications; in the modern age the media include, in addition, radio, television, computers, and information networks.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Substances that influence the course of a chemical reaction by ready combination with free radicals. Among other effects, this combining activity protects pancreatic islets against damage by cytokines and prevents myocardial and pulmonary perfusion injuries.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Inorganic salts of phosphoric acid.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A MADS domain-containing transcription factor that binds to the SERUM RESPONSE ELEMENT in the promoter-enhancer region of many genes. It is one of the four founder proteins that structurally define the superfamily of MADS DOMAIN PROTEINS.
Transport proteins that carry specific substances in the blood or across cell membranes.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Substances used to allow enhanced visualization of tissues.
A series of steps taken in order to conduct research.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Antibodies produced by a single clone of cells.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Signal molecules that are involved in the control of cell growth and differentiation.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Morphological and physiological development of EMBRYOS or FETUSES.
Substances that reduce the growth or reproduction of BACTERIA.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
A ubiquitous sodium salt that is commonly used to season food.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC
A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Glycoproteins which have a very high polysaccharide content.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.

A telomere-independent senescence mechanism is the sole barrier to Syrian hamster cell immortalization. (1/2283)

Reactivation of telomerase and stabilization of telomeres occur simultaneously during human cell immortalization in vitro and the vast majority of human cancers possess high levels of telomerase activity. Telomerase repression in human somatic cells may therefore have evolved as a powerful resistance mechanism against immortalization, clonal evolution and malignant progression. The comparative ease with which rodent cells immortalize in vitro suggests that they have less stringent controls over replicative senescence than human cells. Here, we report that Syrian hamster dermal fibroblasts possess substantial levels of telomerase activity throughout their culture life-span, even after growth arrest in senescence. In our studies, telomerase was also detected in uncultured newborn hamster skin, in several adult tissues, and in cultured fibroblasts induced to enter the post-mitotic state irreversibly by serum withdrawal. Transfection of near-senescent dermal fibroblasts with a selectable plasmid vector expressing the SV40 T-antigen gene resulted in high-frequency single-step immortalization without the crisis typically observed during the immortalization of human cells. Collectively, these data provide an explanation for the increased susceptibility of rodent cells to immortalization (and malignant transformation) compared with their human equivalents, and provide evidence for a novel, growth factor-sensitive, mammalian senescence mechanism unrelated to telomere maintenance.  (+info)

Growth-inhibitory effect of cyclic GMP- and cyclic AMP-dependent vasodilators on rat vascular smooth muscle cells: effect on cell cycle and cyclin expression. (2/2283)

1. The possibility that the antiproliferative effect of cyclic GMP- and cyclic AMP-dependent vasodilators involves an impaired progression of vascular smooth muscle cells (VSMC) through the cell cycle and expression of cyclins, which in association with the cyclin-dependent kinases control the transition between the distinct phases of the cell cycle, was examined. 2. FCS (10%) stimulated the transition of quiescent VSMC from the G0/G1 to the S phase (maximum within 18-24 h and then to the G2/M phase (maximum within 22-28 h). Sodium nitroprusside and 8-Br-cyclic GMP, as well as forskolin and 8-Br-cyclic AMP markedly reduced the percentage of cells in the S phase after FCS stimulation. 3. FCS stimulated the low basal protein expression of cyclin D1 (maximum within 8-24 h) and E (maximum within 8-38 h) and of cyclin A (maximum within 14-30 h). The stimulatory effect of FCS on cyclin D1 and A expression was inhibited, but that of cyclin E was only minimally affected by the vasodilators. 4. FCS increased the low basal level of cyclin D1 mRNA after a lag phase of 2 h and that of cyclin A after 12 h. The vasodilators significantly reduced the FCS-stimulated expression of cyclin D1 and A mRNA. 5. These findings indicate that cyclic GMP- and cyclic AMP-dependent vasodilators inhibit the proliferation of VSMC by preventing the progression of the cell cycle from the G0/G1 into the S phase, an effect which can be attributed to the impaired expression of cyclin D1 and A.  (+info)

Mycophenolate mofetil inhibits rat and human mesangial cell proliferation by guanosine depletion. (3/2283)

BACKGROUND: Mycophenolate mofetil (MMF) is used for immunosuppression after renal transplantation because it reduces lymphocyte proliferation by inhibiting inosine monophosphate dehydrogenase (IMPDH) in lymphocytes and GTP biosynthesis. In the present study we asked if therapeutic concentrations of MMF might interfere with mesangial cell (MC) proliferation which is involved in inflammatory proliferative glomerular diseases. METHODS: Rat and human MCs were growth-arrested by withdrawal of fetal calf serum (FCS) and stimulated by addition of FCS, platelet-derived growth factor (PDGF) or lysophosphatidic acid (LPA). Different concentrations of MMF (0.019-10 microM) were added concomitantly in the presence or absence of guanosine. MC proliferation was determined by [3H]thymidine incorporation. Cell viability was assessed by trypan blue exclusion. Apoptotic nuclei were stained using the Hoechst dye H33258. Cytosolic free Ca2+ concentrations were determined with the fluorescent calcium chelator fura-2-AM. RESULTS: MMF inhibited mitogen-induced rat MC proliferation with an IC50 of 0.45 +/- 0.13 microM. Human MCs proved to be even more sensitive (IC50 0.19 +/- 0.06 microM). Inhibition of MC proliferation was reversible and not accompanied by cellular necrosis or apoptosis. Addition of guanosine prevented the antiproliferative effect of MMF, indicating that inhibition of IMPDH is responsible for decreased MC proliferation. Early signalling events of GTP-binding-protein-coupled receptors, such as changes in intracellular Ca2+ levels were not affected by MMF. CONCLUSIONS: The results show that MMF has a concentration-dependent antiproliferative effect on cultured MCs in the therapeutic range, which might be a rationale for the use of this drug in the treatment of mesangial proliferative glomerulonephritis.  (+info)

Altered expression of the IGF-1 receptor in a tamoxifen-resistant human breast cancer cell line. (4/2283)

The relationship between oestrogen (E2) and insulin-like growth factor-one (IGF-1) was examined in both tamoxifen-sensitive (MCF 7/5-21) and tamoxifen-resistant (MCF 7/5-23) subclones of the MCF 7 cell line. Both subclones were grown in defined, serum-free (SF) medium over a period of 7 days with the addition of E2 or IGF-1 or a combination of both agents. Growth of both MCF 7/5-21 and 7/5-23 cells was stimulated (245% and 350%, respectively) by E2. However, only the growth of MCF 7/5-23 cells was stimulated (266%) by IGF-1. A combination of E2 and IGF-1 significantly enhanced MCF 7/5-21 and 7/5-23 cell growth (581% and 695%, respectively). E2-induced IGF-1 receptor (IGF-1R) levels (as measured by 125I-IGF-1 binding and Northern analyses) in only MCF 7/5-23 cells. This effect was partially inhibited by tamoxifen. In medium containing serum, the growth of only the MCF 7/5-23 cells was significantly inhibited by the IGF-1R monoclonal antibody, alphaIR-3. The detection of E2-induced expression of IGF-2 using RT-PCR was demonstrated in the MCF 7/5-23 cells. These experiments indicate that E2 may sensitize tamoxifen-resistant MCF 7/5-23 cells to the growth stimulatory actions of IGF-2 via up-regulation of the IGF-1R and describes a cell-survival mechanism that may manifest itself as tamoxifen resistance.  (+info)

Protection against necrosis but not apoptosis by heat-stress proteins in vascular smooth muscle cells: evidence for distinct modes of cell death. (5/2283)

We have reported previously that cultured vascular smooth muscle cells (VSMC) isolated from spontaneously hypertensive rats (SHR) show higher proliferation and cell death than normotensive controls. In addition to protecting cells against death, heat stress proteins (HSPs) appear to play a role in cell proliferation. This investigation examines the involvement of HSP72 and HSP27 in altered SHR VSMC proliferation and death. We have performed detailed discriminatory analysis to characterize which type of VSMC death is induced by heat stress (HS) and serum deprivation. Serum deprivation induced apoptosis (caspase-3 cleavage and DNA laddering) and secondary necrosis, the 2 processes being a continuum of each other. In contrast, acute HS (46 degrees C, 30 minutes), which inhibited BN. lx and SHR VSMC proliferation by 2-fold, increased necrosis (by 5-fold and 2-fold, respectively) but not apoptosis. HSP72 and HSP27 expression evoked in VSMC by mild HS (44 degrees C, 15 minutes) 6 hours before acute HS prevented the inhibition of proliferation and induction of necrosis with no effect on serum deprivation-induced or staurosporine-induced apoptosis. This induced expression of HSP72 and HSP27 did not eliminate the higher basal proliferation, apoptosis, and necrosis of SHR VSMC compared with BN.lx VSMC, suggesting that these HSPs are not involved in altered SHR VSMC proliferation and death. Also, although apoptosis and necrosis may be a continuum, in VSMC the 2 processes may be distinguished by HS, in which only necrosis is prevented by prior HSP accumulation. This observation may be of use in designing strategies for cellular protection.  (+info)

O-glycosylation potential of lepidopteran insect cell lines. (6/2283)

The enzyme activities involved in O-glycosylation have been studied in three insect cell lines, Spodoptera frugiperda (Sf-9), Mamestra brassicae (Mb) and Trichoplusia ni (Tn) cultured in two different serum-free media. The structural features of O-glycoproteins in these insect cells were investigated using a panel of lectins and the glycosyltransferase activities involved in O-glycan biosynthesis of insect cells were measured (i.e., UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP-Gal:core-1 beta1, 3-galactosyltransferase, CMP-NeuAc:Galbeta1-3GalNAc alpha2, 3-sialyltransferase, and UDP-Gal:Galbeta1-3GalNAc alpha1, 4-galactosyltransferase activities). First, we show that O-glycosylation potential depends on cell type. All three lepidopteran cell lines express GalNAcalpha-O-Ser/Thr antigen, which is recognized by soy bean agglutinin and reflects high UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase activity. Capillary electrophoresis and mass spectrometry studies revealed the presence of at least two different UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases in these insect cells. Only some O-linked GalNAc residues are further processed by the addition of beta1,3-linked Gal residues to form T-antigen, as shown by the binding of peanut agglutinin. This reflects relative low levels of UDP-Gal:core-1 beta1,3-galactosyltransferase in insect cells, as compared to those observed in mammalian control cells. In addition, we detected strong binding of Bandeiraea simplicifolia lectin-I isolectin B4 to Mamestra brassicae endogenous glycoproteins, which suggests a high activity of a UDP-Gal:Galbeta1-3GalNAc alpha1, 4-galactosyltransferase. This explains the absence of PNA binding to Mamestra brassicae glycoproteins. Furthermore, our results substantiated that there is no sialyltransferase activity and, therefore, no terminal sialic acid production by these cell lines. Finally, we found that the culture medium influences the O-glycosylation potential of each cell line.  (+info)

Flow cytometric cell cycle analysis of cultured porcine fetal fibroblast cells. (7/2283)

Normal development of nuclear transfer embryos is thought to be dependent on transferral of nuclei in G0 or G1 phases of the cell cycle. Therefore, we investigated the cell cycle characteristics of porcine fetal fibroblast cells cultured under a variety of cell cycle-arresting treatments. This was achieved by using flow cytometry to simultaneously measure cellular DNA and protein content, enabling the calculation of percentages of cells in G0, G1, S, and G2+M phases of the cell cycle. Cultures that were serum starved for 5 days contained higher (p < 0.05) percentages of G0+G1 (87.5 +/- 0. 7) and G0 cells alone (48.3 +/- 9.7) compared with rapidly cycling cultures (G0+G1: 74.1 +/- 3.0; G0: 2.8 +/- 1.2). Growth to confluency increased (p < 0.05) G0+G1 percentages (85.1 +/- 2.8) but did not increase G0 percentages (6.0 +/- 5.3) compared to those in cycling cultures. Separate assessment of small-, medium-, and large-sized cells showed that as the cell size decreased from large to small, percentages of cells in G0+G1 and G0 alone increased (p < 0.05). We found 95.2 +/- 0.3% and 72.2 +/- 12.0% of small serum-starved cells in G0+G1 and G0 alone, respectively. Cultures were also treated with cell cycle inhibitors. Treatment with dimethyl sulfoxide (1%) or colchicine (0.5 microM) increased percentages of cells in G0 (24.8 +/- 20.0) or G2+M (37.4 +/- 4.6), respectively. However, cells were only slightly responsive to mimosine treatment. A more complete understanding of the cell cycle of donor cells should lead to improvements in the efficiency of nuclear transfer procedures.  (+info)

Cellular effects of beta-particle delivery on vascular smooth muscle cells and endothelial cells: a dose-response study. (8/2283)

BACKGROUND: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact cellular alterations induced by beta irradiation remain to be elucidated. METHODS AND RESULTS: We investigated in vitro the ability of 32P-labeled oligonucleotides to alter (1) proliferation of human and porcine vascular smooth muscle cells (VSMCs) and human coronary artery endothelial cells (ECs), (2) cell cycle progression, (3) cell viability and apoptosis, (4) cell migration, and (5) cell phenotype and morphological features. beta radiation significantly reduced proliferation of VSMCs (ED50 1.10 Gy) and ECs (ED50 2.15 Gy) in a dose-dependent manner. Exposure to beta emission interfered with cell cycle progression, with induction of G0/G1 arrest in VSMCs, without evidence of cell viability alteration, apoptosis, or ultrastructural changes. This strategy also proved to efficiently inhibit VSMC migration by 80% and induce contractile phenotype appearance, as shown by the predominance of alpha-actin immunostaining in beta-irradiated cells compared with control cells. CONCLUSIONS: 32P-labeled oligonucleotide was highly effective in inhibiting proliferation of both VSMCs and ECs in a dose-dependent fashion, with ECs showing a higher resistance to these effects. beta irradiation-induced G1 arrest was not associated with cytotoxicity and apoptosis, thus demonstrating a potent cytostatic effect of beta-based therapy. This effect, coupled to that on VSMC migration inhibition and the appearance of a contractile phenotype, reinforced the potential of ionizing radiation to prevent neointima formation after angioplasty.  (+info)

title: Galpha12 Protects Vascular Endothelial Cells from Serum Withdrawal-Induced Apoptosis through Regulation of miR-155, doi: 10.3349/ymj.2016.57.1.247, category: Article
Japans largest platform for academic e-journals: J-STAGE is a full text database for reviewed academic papers published by Japanese societies
|p||strong|Technical Advantage:|/strong|  This proprietary formulation is not available from other suppliers.|br /| Corning Insectagro DS2 Serum-Free/Protein-Free Medium was developed for the growth and maintenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression.  At the optimal temperature range 22-24ºC, DS2 cells grow as a loose monolayer and are readily adaptable to growth in suspension.  Under these conditions, the cells require minimal adaptation to serum-free culture.|br /| - Complete Certificate of Analysis available for each production lot, along with full formulation information|br /| - Produced under the highest industry standards to ensure superior results|/p|
Most convenient Xeno-free and Serum-free culture media for all your human cell culture needs. Xeno-free, serum-free, and chemically defined cell culture media for supporting the culture of human stem cells and regular cells in 2D and 3D. This series of products replace human or animal derived products, such as human and animal serum, and human and animal derived supplements. All our cell culture medium products are packed in 10 convenient 50mL freezable medium bottles. No need to aliquot the big bottle anymore. Take one small bottle of medium to use and throw the remaining bottles in freezer for long term storage without worrying about the degradation of the medium components.. ...
This study points out the interest in HPL as a replacement for FBS in culture media for expansion of human BM MSCs. Thus, HPL-containing media not only preserve their phenotype as well as their differentiation capacity but also shorten culture time by increasing their growth rate. Nevertheless, some differences exist in terms of cytokines produced, suggesting functional differences between MSCs expanded in media supplemented with HPL and FBS. This has to be considered for particular clinical applications.. The possibility to use animal serum-free culture media has been reported in several recent studies by substituting FBS with human-derived supplements such as HPL or human serum [17-25]. In the present study, MSC expansions were performed in three different HPL-supplemented media consisting of BGM (α-MEM) with (M2) or without (M3 and M4) FBS and compared with the standard medium devoid of HPL (M1). M1 (supplemented with 10% FBS + 1 ng/mL FGF2) represents the reference medium for MSC expansion ...
A central feature of tumor metastasis is the migration of malignant cells through interstitial tissues and vascular structures as they spread throughout the body. Various components of the extracellular matrix and of basement membranes, consisting of genetically distinct collagens, proteoglycans, and noncollagenous glycoproteins, are known to modulate certain aspects of cell behavior, including cell movement. Serum spreading factor is a glycoprotein component of human serum that is also found in interstitial tissues. Two native forms are seen in human serum, a Mr 65,000 and a Mr 75,000 component. Spreading factor promotes substratum attachment and spreading of diverse cell types, including epithelial and fibroblastic cells, and will affect the growth rate and differentiation of cells in serum-free culture media. Serum spreading factor was shown to promote the directed migration of the following tumor cell lines in modified Boyden chamber assays: murine melanoma K-1735 (clones M2, M4, and 16); ...
In my experience with serum-free media, cell lines that were normally adherent did not adhere, and did not grow well (Cho1.1, H460, 293T); however, cells that would grow in clumps were more clumpy, but grew slowly (HepG2, H157). I do not know what these changes is growth behavior and apparence have on subsequent experiments, but I would guess it varies with cell lines ...
Immunofluoresence Transferrin-Uptake Assay. Incubate cells in serum free medium 1hr-0/n. Apply 25 g/ml biotinylated holo-transferrin (Sigma T-3915, from stock 1 mg/ml) in pre-warmed serum free medium to cells and incubate at 37 C for the desired time (15-20 minutes for steady state).. Rinse briefly with PBS. Fixation temperature may range from 4 C to 37 C, depending on other stains. For transferrin alone, use 4 C.. Important: Fix cells with 3.2 % paraformaldehyde pre-equilibrated in serum-free medium at the required temperature for 20 min.. Rinse with PBS and quench for 10 min with 50 mM NH4Cl in PBS. Permeabilise and block with TBS + 0.2 % Saponin + 10 % serum derived from the animal species in which the 2nd antibody was raised for 15 min.. Use 1:10 dilution of above dilution in TBS (0.02 % Saponin + 1 % serum) to make a 1:500 dilution of Streptavidin from Molecular Probes conjugated to the fluorophor of choice (e.g. Alexa 546). Don t use more than 1:500. Incubate cells for 0.5 -1 hour or o/n ...
PromoCells Primary Cancer Culture System is a standardized, animal component-free and serum-free system for the isolation and culture of human primary tumor cells. It supports the long-term culture of cancer cells while maintaining the original tumor properties and heterogeneity with controlled stroma support. Prolonged culture allows for functional selection of malignant cells giving access to an enriched population of primary cancer cells.. An innovative solution for culturing primary cancer cells: ...
Assay for determining the IC50 of a drug! - posted in Cell Biology: HI there, I am confused that most of the researchers to investigate the IC50 of a particular drug will consider to use serum free medium. After obtaining the IC50 concentration, they did not mention whether they were using serum free medium to perform other assay, such as Cell cycle assay...! I am concerning the issue that when to use serum free medium is suitable? Such that if you perform the Cell cyc...
Inclusion body, protein reagents, endotoxin removal, protein clean-up, abundant serum depletion, on-column proteolytic digestion, and urine protein purification.. ...
Affiliation:横浜市立大学,生命ナノシステム科学研究科,教授, Research Field:Cell biology,Fisheries chemistry,Obstetrics and gynecology,Biological pharmacy,広領域, Keywords:浸潤,転移,細胞外マトリックス,癌,human,extracellular proteinase,serum-free culture,serum-independent,cell line,細胞接着因子, # of Research Projects:11, # of Research Products:27
MesenCult™ Proliferation Kit (Human) is a standardized, serum-containing medium for the culture of human MSCs using CFU-fibroblast assays.
Mesenchymal stem cell (MSC) transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium, but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment. The aim of this stud
We are developing a long term culture protocol (several weeks) in which serum is replaced at the level of the culture medium, trypsin inhibition and the cryopreservation medium. After a preliminar screening to identify the essential factors for the MRC-5 cell proliferation, a prototype medium is now tested in long term cultures and the cell culture conditions are under optimalization. Afterwards, the cells adapted to the serum free medium will be further characterized at the molecular level ...
This can lead to under conditions in the fluid on the left, the base of the lithotriptor is even more fear, which in turn is the mainstay of treatment today. The dissection is very complex. A short period of time, usually for a few children, mostly girls, who exhibit modics, this technique, because it is important to remind the survivor s reactions to serum deprivation-induced apoptosis. Cameron could never live their lifestyle on her mouth, but then. While increased number of benzodiazepines with longer half-lives are cleared from the pulmonary circulation and blocks its actions. 5.4b). Urology. Therapy if there is an ingredient of a limp should be consulted for further information on the desired effect, v-loctm has been taken or. Do not measure the patient should peak- ow readings. Classic results from a cathode ray tube to provide an example. Elevated liver transaminase concen- that it is import- on the prevailing circumstances. Bronchitis, asthma, emphysema). 2010;42:391 6. 7. Relative from ...
Principal Investigator:KIKUCHI Aiko, Project Period (FY):1985 - 1986, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Neurology
Serum-Free media for cell cultures from Biological Industries include chemically-defined, protein-free, and serum-free media formulations for mammalian and stem cell cultures.
Cell culture media that does not contain a nutrient and growth factor-rich serum derived from animal blood. Serum-free media uses synthetic or purified ingredients to provide nutrients and growth factors that support growth and survival of cells in culture.
Inhibition of PI3K in serum-starved LNCaP cells induces apoptosis that is abrogated by introduction of EGF or serum. LNCaP cells serum-starved for 20 h were treated with fresh medium containing 10% serum (serum), serum-free medium (No serum), medium containing 10% serum and 25 μ M LY (LY+serum), serum-free medium with 25 μ M LY (LY) or serum-free medium containing 25 μ M LY and 100 ng/ml EGF (LY+EGF) for 6, 12 and 20 h before (a) cell death was determined by propidium iodide staining and (c) caspase 3 and (d) caspase 9 activity as described in Materials and methods. Percentage of cells in sub-G1 was analysed using the WINMDI 2.8 software. DNA histograms depicting % cell in sub-G1 of one of the experiments after 20 h incubation in different treatments is shown in (b). Results shown in (a), (c) and (d) are a mean of three experiments performed in duplicates±s.e. ...
Furthermore, in vitro culture is never a physiological environment, so it all depends on how much you want to be able to control your experiment conditions - cells usually do better in a medium that has been supplemented with serum, but culturing them in a serum-free environment allows you to determine the conditions of your experiement much more precisely ...
Defective acute regulation of hepatic glycogen synthase by glucose and insulin, caused by severe insulin deficiency, can be corrected in adult rat hepatocytes in primary culture by inclusion of insulin, triiodothyronine, and cortisol in a chemically defined serum-free culture medium over a 3-day period (Miller, T. B., Jr., Garnache, A. K., Cruz, J., McPherson, R. K., and Wolleben, C. (1986) J. Biol. Chem. 261, 785-790). Using primary cultures of hepatocytes isolated from normal and diabetic rats in the same serum-free chemically defined medium, the present study addresses the effects of cycloheximide and actinomycin D on the chronic actions of insulin, triiodothyronine, and cortisol to facilitate the direct effects of glucose on the short-term activation of glycogen synthase. The short-term presence (1 h) of the protein synthesis blockers had no effect on acute activation of glycogen synthase by glucose in primary hepatocyte cultures from normal rats. Normal cells maintained in the presence of
Cervical explant cultures were first developed by Fink et al.44 for the study of epithelium metaplasia in vitro. They consisted of large tissue explants (5 × 5 × 2 mm or 5 × 10 × 3 mm) cultured on a thin slab of agarose-gelled serum-free Eagles Basal Medium on top of a stainless steel supporting grid. This culture method was successfully adapted to the study of HSV-2 and HSV-1 infection in vitro and was shown to support the replication of these two human herpesviruses.45 OBrien et al.46 modified this method for the study of the production of glycoprotein from normal and malignant cervical explants of smaller size (5 mm3) cultured either fully immersed in serum-free culture medium or maintained at the air-liquid interface, supported only by a stainless steel grid mesh. On the basis of glycoprotein production, the authors concluded that the grid technique was superior to the immersion culture. It was this grid technique that was adapted to the study of HIV-1 infection in human cervical ...
In this study, we characterized signaling cascades activated on direct contact of leukemic cells with bone marrow-derived stromal cells. Our findings indicate stroma-induced activation of ILK with phosphorylation of Akt and GSK3β. Because p-GSK3βSer9 is a known cellular target of ILK ( 7), these results are consistent with evidence of ILK activation induced by growth factors or engagement of the integrins by stroma. We further showed that the nuclear accumulation of β-catenin, a downstream GSK3β target, was induced in NB4 cells by coculture with MSCs and that this accumulation was abrogated when the PI3K/ILK pathway was inhibited. In contrast, the GSK3 inhibitor BIO, which prevented the serum withdrawal-induced apoptosis of NB4 cells, stimulated the translocation of β-catenin to the nucleus in cells growing without stromal support. Altogether, these findings provide evidence that stroma-induced ILK activation results in GSK3 inhibition and up-regulation of nuclear β-catenin consistent with ...
Wnt5a Does Not Support Hematopoiesis in Stroma-Free, Serum-Free Cultures. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Staurosporine inhibits apoptotic blebbing in PC12 and COS-7 cells. Serum removal and z-VAD-FMK treatment were performed as described in the legend to Fig. 1.
Rat (Adipose Derived) Stem Cell Media (Serum Free Medium or Medium with Serum). This product is tissue tested including Stem Cells and is available as 500ml sterile filtered unit. The product is also available as a pack of 6, 500ml unit sizes ...
Human Mesenchymal (Liver) Stem Cell Media (Serum Free Medium or Medium with Serum). This product is tissue tested including Stem Cells and is available as 500ml sterile filtered unit. The product is also available as a pack of 6, 500ml unit sizes ...
BioAssay record AID 89632 submitted by ChEMBL: Cell viability of serum-deprived Jurkat cells were determined, 24 hours after incubation with 100 uM concentration of the compound, by MTT-assay.
In this webinar we discuss the negative effects of culturing cells in serum and you will learn about the advantages of switching to serum-free cell culturing.In cell biology, cells are...
TY - JOUR. T1 - Sphingosine 1-Phosphate Protects Human Umbilical Vein Endothelial Cells from Serum-deprived Apoptosis by Nitric Oxide Production. AU - Kwon, Young Guen. AU - Min, Jeong Ki. AU - Kim, Ki Mo. AU - Lee, Doo Jae. AU - Billiar, Timothy R.. AU - Kim, Young Myeong. PY - 2001/4/6. Y1 - 2001/4/6. N2 - Sphingosine 1-phosphate (S1P) can prevent endothelial cell apoptosis. We investigated the molecular mechanisms and signaling pathways by which S1P protects endothelial cells from serum deprivation-induced apoptosis. We show here that human umbilical vein endothelial cells (HUVECs) undergo apoptosis associated with increased DEVDase activity, caspase-3 activation, cytochrome c release, and DNA fragmentation after 24 h of serum deprivation. These apoptotic markers were suppressed by the addition of S1P, the NO donor S-nitroso-N-acetylpenicillamine (100 μM), or caspase-3 inhibitor z-VAD-fmk. The protective effects of S1P were reversed by the nitric-oxide synthase (NOS) inhibitor ...
MatTeks Normal Human Bronchial Epithelial Cells (NHBE) provide an ideal serum-free culture system to study cell-cell and cell-matrix interactions, drug
Recently, cell lifestyle systems producing hepatitis C virus particles (HCVcc) were developed. we developed serum-free culture systems producing high-titer single-density sf-HCVcc, showing similar biological properties as HCVcc. This methodology has the potential to advance HCV vaccine development and to facilitate biophysical studies of HCV. within the family. Due to a high degree of genetic heterogeneity, HCV has been categorized in 6 essential genotypes and several subtypes epidemiologically, differing in around 30% and 20% of their nucleotide and amino Vorinostat acidity sequence, [3 respectively,4]. Genotypes display important biological and clinical variations [5C10]. Serotypes Rabbit Polyclonal to 5-HT-2C. never have been defined; nevertheless, different genotypes and subtypes display differential level of sensitivity to neutralizing antibodies within sera of chronically contaminated patients also to monoclonal neutralizing antibodies with restorative potential [6,11C14]. The 9.6 kb HCV ...
The HL-60 (Human promyelocytic leukemia cells) cell line has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at MD Anderson Cancer Center. HL-60 cells are predominantly a neutrophilic promyelocyte (precursor). Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this line, differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide (DMSO), or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 ...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 30 Nov 2017. Apply now!. ...
The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturin
For typical Western blotting experiments, (e.g., Fig. 2J-L), cells were plated at 200,000 cells per well of a 12-well plate in complete Dulbeccos modified Eagles medium (DMEM)/F12 media containing 10% serum. The next day, cells were treated as indicated. For Western blotting involving production of cytokines, ARPE-19 cells were plated as described above followed by gradual serum removal over the course of a week until the serum was removed completely followed by indicated treatments. At appropriate time points, ARPE-19 cells were lysed in-plate with either radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris, pH 7.4, 150 mM NaCl2, 1% Triton X-100 [vol/vol], 0.1% SDS [wt/vol], 0.5% sodium deoxycholate [wt/vol]) supplemented with Halt Protease and Phosphatase inhibitors (Pierce, Rockford, IL, USA) and benzonase (Sigma-Aldrich Corp.) or a buffer containing 50 mM HEPES pH 8, 10 mM KCl, 2 mM MgCl2, and 1.0% SDS also supplemented with benzonase. Cells were lysed at RT for approximately 1 to 2 ...
Human Cardiomyocyte Perfusion Serum Free Media. This Product is also available with Serum Cat# P36044-15S This product would require and Human Cardiomyocytes Cat# 36044-15 This product is tissue culture tested including Stem Cells and is.... ...
Application: ELISA systems, In vitro neutralization, Immunohistochemistry and Western blot analysis. Clone: DB-14 Isotype: Mouse IgG|sub|2a Production: In vitro using serum free medium. Purification: Ion exchange chromatography. Packaging: Lyophilized and vacuum-packed. Contents: 0.5 mg/vial Buffer: Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose. Specificity: Neutralizes both natural and recombinant rat IFN-gamma in vitro. The antibody does not cross-react with mouse and human IFN-gamma and does not bind to rat IFN-alpha and -beta.
The ultrastructure of bovine morulae and blastocysts developed from in vitro-matured and -fertilized oocytes in a serum-supplemented medium was compared with that of morulae and blastocysts collected
A. PRIME-XV MSC Expansion SFM outperforms leading competitors in cell expansion studies while maintaining MSC gross morphology, cell surface expression markers, multipotency potential and immune modulation abilities. Additionally, PRIME-XV MSC Expansion SFM has been tested to support optimal cell expansion of different sources of MSCs, including bone marrow-derived and adipose-derived MSCs. Little to no adaptation is required when shifting from a serum-containing medium to our medium. PRIME-XV MSC Expansion SFM is ready-to-use with no additional growth supplements required and is supplied in a convenient one 250mL complete component. ...
Cornings comprehensive line of standard and custom media helps create an optimal environment for all stages of cell culture growth and scaling.
Xeno-Free, Serum-Free Medium Engineered for the culture and expansion of lymphocytes Research Use Only 1 L Bottle Standard Size Scientific and Regulatory...
Cells wont adhere to the coverslip without serum, so grow them first for 24 hours in regular medium.. Remove the medium, replace with serum-free medium. Fix the coverslips 24 hours later.. ...
Effects of murrayafoline A on the PDGF-BB-stimulated activation of PDGF-Rβ, PLCγ1, Akt, ERK1/2, and STAT3. Quiescent VSMCs cultured in serum-free medium were
UltraCULTURETM Serum-free Cell Culture Medium, without L-Glutamine, 500 mL. NOTE: This product is temporary unavailable please contact our scientific support team for alternatives ...
Abstract: Stochastic partial differential equations frequently arise as limits of finite systems of weighted interacting particles. For a variety of purposes, it is useful to keep the particles in the limit obtaining an infinite exchangeable system of stochastic differential equations for the particle locations and weights. The corresponding de Finetti measure then gives the solution of the SPDE. These representations frequently simplify existence, uniqueness and convergence results. Following some discussion of general approaches to SPDEs, the talk will focus on situations where the particle locations are given by an iid family of diffusion processes, and the weights are chosen to obtain a nonlinear driving term and to match given boundary conditions for the SPDE. (Recent results are joint work with Dan Crisan ...
Easy to read snippets and explanations on dermatology research. Learn about the role of diet in acne and the debate over paper or electronic prescriptions.
TY - JOUR. T1 - Angiopoietin-1 protects endothelial cells from hypoxia-induced apoptosis via inhibition of phosphatase and tensin homologue deleted from chromosome ten. AU - Lee, Sae Won. AU - Youn, Seock Won. AU - Kim, Tae Youn. AU - Suh, Jung Won. AU - Koh, Gou Young. AU - Kwon, Yoo Wook. AU - Chae, In Ho. AU - Park, Young Bae. AU - Kim, Hyo Soo. PY - 2009/2. Y1 - 2009/2. N2 - Background and Objectives: Angiopoietin-1 (Ang1) is a regulator of blood vessel growth and maturation, and prevents radiation-induced or serum deprivation-induced apoptosis. Phosphatase and tensin homologue deleted from chromosome ten (PTEN), a well-known tumor suppressor, regulates cell cycle arrest and apoptosis. Hypoxia induces apoptosis by increasing the expression of PTEN. We hypothesized that Angl may regulate PTEN expression and, thus, reduce endothelial apoptosis under hypoxia in vitro and in vivo. Materials and Methods: In vitro, human umbilical vein endothelial cells (HUVECs) were treated with Ang1, and ...
Human SDMCs. Cultures of skin-derived mast cells (SDMCs) were prepared as previously described (16). In brief, discarded surgical samples of human skin were obtained from deidentified sources through the Cooperative Human Tissue Network (supported by the National Cancer Institute, NIH). Skin fragments were mechanically minced and then processed via enzymatic digestion with Collagenase Type 2 (Worthington Biochemical Corp.), DNase Type 1 (MilliporeSigma), and hyaluronidase (MilliporeSigma) before isolation of mononuclear cells via centrifugation with Percoll PLUS (GE Healthcare). Single-cell suspensions were cultured for 4 weeks in X-Vivo15 Serum-Free Culture Medium (Lonza) containing 100 ng/mL recombinant human stem cell factor (SCF; PeproTech). Culture purity was assessed via flow cytometry for cKit and FcεRI expression.. Degranulation and cytokine assays. Lyophilized stocks of the BTKis ibrutinib (Selleckchem), acalabrutinib (provided by Acerta Pharma, a member of the AstraZeneca Group, San ...
Two purified serum protein fractions, fetuin and serum albumin, will replace whole or dialyzed serum in supporting the growth of single S3 HeLa cells in an otherwise chemically defined nutrient solution.. In the serum-free medium, single S3 cells will form macroscopic colonies with essentially 100 per cent efficiency.. The generation time of S3 cells in the serum-free medium is approximately 50 per cent greater than that observed in an optimal, serum-containing medium.. All components of the serum-free medium are available commercially, except fetuin, which can easily be prepared in substantial quantities.. The problem of the purity of the protein preparations and of their possible roles in promoting cell growth is discussed.. ...
Abstract: From the emerging field of cellular therapy, there is an increasing demand for new media to provide optimal expansion of cells in culture. Hematopoietic stem cells have complex growth requirements and serum free media include over 50 components at concentrations from near zero to 7 g/L. Conventionally, media are developed empirically, either by depletion analysis or component addition to determine if culture productivity can be increased. This optimization process consumes valuable time and labour. We are investigating medium optimization through monitoring the gene expression profile of cells in culture based on the hypothesis that cells experiencing a particular limitation will exhibit a characteristic gene expression profile. We have analyzed gene expression of human TF-1 cells under nutritional stress and literature data on yeast under amino acid limitations. The expression levels of genes in pathways relevant to these stresses were monitored at several time points following ...
The monoclonal antibody to human TNF-alpha (clone B-F7) recognizes both native and recombinant human TNF-alpha. The B-F7 antibody is suitable for ELISA and ELISPOT assays. Produced In vitro using serum free medium.
InVitria offers a portfolio of recombinant proteins, serum-free cell culture components and blood-free cell culture media for development and large scale production of medical devices and biologics such as vaccines, gene therapy and cell therapy.
Make use of our cell line adaptation service and we adapt your adherently growing cell line to serum-free growth in suspension - For scalable biologi…
By conducting the entire media development project in our laboratories, we are able to deliver the most improvement in the shortest amount of time. Once we receive the primary or stem cells, we will perform all of the research necessary to discover the optimal media for our customers process. Beginning with either a customer-owned formula or with our own proprietary media, we will develop growth or differentiation media designed explicitly to meet each customers specific process requirements such as serum-free, xeno-free, animal-component-free, growth rate, differentiation efficiencies, etc.. ...
Support cell culture growth and biomanufacturing processes with IMDM media. Shop Cytiva HyClone™ Iscoves Modified Dulbeccos Medium (IMDM) at
We established a serum-free organ culture system of isolated single vibrissa rudiments taken from embryonic day 13 mice. This… Expand ...
Serum-free culture media. Culture media is an instrumental part of cellular agriculture and generating cultured tissue because ... These stem cells are then immersed into a culture medium so that they can proliferate. Culture media consist of basal media, ... Instead, insect cell medium typically uses a basal medium (such as Eagle's Medium, Grace's Insect Medium or Schneider's ... As a result, the natural starting point is combining Fetal Bovine Serum (FBS) into the culture media. FBS is somewhat ...
"Serum-free Cell Culture: The Serum-free Media Interactive Online Database". Altex. 27 (1): 53-62. doi:10.14573/altex.2010.1.53 ... Organoids grow in vitro on scaffolds (biological or synthetic hydrogels such as Matrigel) or in a culture medium. Organoids are ... "Go cruelty free" The launch of the global "Go Cruelty-Free" campaign occurred in 2012 and every year since the launch there ... Cell culture can be an alternative to animal use in some cases. For example, cultured cells have been developed to create ...
... serum-free and chemically defined media (CDM) have been developed. The effectiveness of serum-free media is limited, however as ... The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and ... Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to ... June 2010). "Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods ...
The culture medium used to grow Raji cells is RPMI supplemented with serum. Some characteristics of Raji cells include a lack ... Raji cells grow as single, non-motile, free-floating (non-adhesion) individuals or doublets to glass. Some cells look elongated ... Pulvertaft, R. J. V. (1 May 1965). "A study of malignant tumours in Nigeria by short-term tissue culture". Journal of Clinical ... The cells are relatively small in diameter (5-8 μm), have irregular indented nuclei, and almost extensive cytoplasm with free ...
... , also known as RPMI medium, is a growth medium used in cell culture. RPMI 1640 was developed by George E. Moore, ... RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate ... v t e (Cell culture media, All stub articles, Cell biology stubs). ... ...
... may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of ... may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media. ... laidlawii has been identified as a common contaminant of growth media for cell culture. A. laidlawii was first isolated from ... Windsor HM; Windsor GD; Noordergraaf JH (March 2010). "The growth and long term survival of Acholeplasma laidlawii in media ...
GenClone provides cell culture media, sera (FBS), buffers, and reagents for cell and tissue culture. Nutri-fly provides ... TITAN provides powder-free nitrile and latex examination gloves. UPrep provides spin columns for DNA and RNA (nucleic acid) ... a full line of cell culture media products centered around ultra-pure, high-performance fetal bovine sera.[citation needed] The ... 5867964) GenClone, High-performance cell culture media products (Reg. #: 5322208 ) Gene Choice, Competent cells for cloning ( ...
In culture, cells are surrounded by contaminants. Bovine serum from cell culture media and cellular debris can contaminate the ... To remove these contaminants, cells can be washed with PBS or serum-free medium (SFM) before incubating in SFM and collecting ... Some proteins are secreted in low abundance and then diluted further in the cell culture medium or body fluid, making these ... Supernatant from cells grown in normal medium and cells grown in medium with stable-isotope labeled amino acids is mixed in a 1 ...
"C'est officiel : le Mérovingien sera de retour dans Matrix 4". Konbini - All Pop Everything : #1 Media Pop Culture Chez les ... She says she won't risk Io to help him free Trinity, and has him locked in his quarters. Bugs and her crewmates free Neo and ... A group of rebels, with the help of a programmed version of Morpheus, free Neo from a new version of the Matrix and fight a new ... Seeking to avenge her parents' deaths at the hands of the machines, Sati helps devise a plan to free Trinity. Back in the ...
January 2022). "A serum-free media formulation for cultured meat production supports bovine satellite cell differentiation in ... "Mosa Meat: Dutch Pioneer Slashes Animal-Free Fat Medium Costs By Over 65 Times". Green Queen. 18 May 2021. Retrieved 1 February ... "Mosa Meat: Dutch Pioneer Slashes Animal-Free Fat Medium Costs By Over 65 Times". Green Queen. 18 May 2021. Retrieved 20 ... In 2020, Mosa Meat announced an 88 times cost reduction of their medium (the broth that feeds the cells) and in 2021, Mosa Meat ...
It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems. FGF2, in ... Additionally, FGF2 is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the ... Soulet F, Al Saati T, Roga S, Amalric F, Bouche G (November 2001). "Fibroblast growth factor-2 interacts with free ribosomal ... "A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells ...
They focused on developing a standard serum-free culture media that would be valuable to all cell types included in the device ... In static cultures, used in traditional skin equivalents, cells receive nutrients in the medium only through diffusion, whereas ... The cells were linked in a 2D fluidic network with culture medium circulating as a blood surrogate, thus efficiently providing ... Microfluidic cell culture systems such as micro cell culture analogs (μCCAs) could be used in conjunction with PBPK models. ...
Towell's II culture chamber). Since serum was found to be toxic, serum-free media were used, and the special apparatus ... Media solidified with agar are also used for organ culture and these media consist of 7 parts 1% agar in BSS, 3 parts chick ... The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ ... Defined media with or without serum are also used with agar. The medium with agar provides the mechanical support for organ ...
"Serum replacement with a growth factor-free synthetic substance in culture medium contributes to effective establishment of ... Within molecular and cell biology, KOSR, knockout serum replacement, is a defined serum-free formulation optimized to grow and ... cells than fetal bovine serum, previously the standard for nutritive supplementation of ES and iPS cells. KOSR has been shown ... Lee, S.; Oh, S.; Kim, D.; Han, J.; Moon, ...
... as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this ... The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid ... HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 ... Breitman, T, S. Collins, B. Keene (1980). "Replacement of serum by insulin and transferrin supports growth and differentiation ...
The S2 cells have been shown to grow up to 5.1×107 cells/ml in serum free medium and above 107 cells/ml in basal media such as ... Several media have been developed for culturing insect cell lines with many of them suitable for culturing S2 cells. ... S2 cells were derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos by Dr. Imogene ... and they can be grown in the absence of serum. ...
FBS-free cell culture media, e.g. with platelet lysate or chemically defined/ animal component free, are used for cell therapy ... Laboratory use of serum Laner‑Plamberger et al. J Transl Med (2015) 13:354 Iudicone P (Jan 2014). "Pathogen-free, plasma-poor ... Platelet lysate is commonly used for supplementation of basal media in mesenchymal stem cells culture. Prior the use, the ... The included clotting factors require to add heparin to the cell culture media to prevent coagulation during incubation. ...
... culture media MeSH D27.720.470.305.250 - culture media, conditioned MeSH D27.720.470.305.255 - culture media, serum-free MeSH ... free radical scavengers MeSH D27.505.519.249 - chelating agents MeSH D27.505.519.249.410 - iron chelating agents MeSH D27.505. ...
8 days of culture in a serum-free medium 8 days of culture in a serum-free medium with activin A 4 days of culture on membranes ... The oocytes are then cultured in media containing nutrients important for oocyte survival, such as gonadotrophins, growth ... In IVM, immature oocytes are removed from the antral follicle of a woman and then are matured in vitro in a culture rich in ... Improving the culture conditions may increase the maturation rates and the potential of IVM oocytes. Besides that, in mouse ...
... providing for serum-free cell culture media in the manufacturing process of cultured meat. A 2021 publication showed that ... Precision fermentation of genetically modified microorganisms may be used to manufacture proteins needed for cell culture media ... In this experiment all the components of the medium are removed one at a time and their effects on the medium are observed. ... In the production of wine, the medium is grape must. In the production of bio-ethanol, the medium may consist mostly of ...
... culture media, conditioned MeSH E07.206.255 - culture media, serum-free MeSH E07.222.210 - dental articulators MeSH E07.222.250 ...
... and the 4 seeding medium is then removed and replaced with the experimental culture medium (phenol red free DMEM with charcoal ... with fetal bovine serum (FBS) and phenol red as buffer tracer (culture medium), at 37 °C, in an atmosphere of 5% CO₂ and 95% ... and treated only with hormone-free medium as a negative control. The bioassay ends on day 6 (late exponential phase) by ... To accomplish the E-SCREEN assay the cells are trypsinized and plated in well culture plates. Cells are allowed to attach for ...
Fetal bovine serum (FBS) is rich in growth factors and is frequently added to growth media used for eukaryotic cell culture. A ... Look up serum or blood serum in Wiktionary, the free dictionary. Blood (Articles with short description, Short description is ... Blood fractionation Globulin Human serum albumin Lipid Serum iron Serum protein electrophoresis Serum-separating tube Serum ... "serum". The Free Dictionary. Retrieved 2019-10-06. Kaplan L (2005-10-06). "Serum Toxicology" (PDF). Clinical Pathology/ ...
... such as blood sera, bioreactor fluid or cell culture media. Contrary to refractometric methods for label-free biomolecular ... "Focal Molography - an optical method for label-free detection of biomolecular interactions".{{cite web}}: CS1 maint: url-status ... is a biophysical method for robust and sensitive detection of biomolecular interactions in a label-free manner. The new method ... label-free trace analysis of a targeted molecule (called biomarker) in complex samples, ...
... been used to isolate and expand CNS stem cells by its ability to aggregate and proliferate hmNPCs under serum-free media ... There are two ways to culture the hmNPCs, the adherent monolayer and the neurosphere culture systems. The neurosphere culture ... NSCs can be cultured in vitro as neurospheres. These neurospheres are composed of neural stem cells and progenitors (NSPCs) ... Media related to Neural stem cells at Wikimedia Commons (CS1 maint: uses authors parameter, Wikipedia articles needing page ...
Primary isolates were cultured in ATCC medium 98 agar containing 105 U penicillin G 1−1, 10 5 U polymyin B 1 −1, 65 mg ... Isolates were grown in broth medium with large (20%) and minute (0.2%) amounts of fetal bovine serum as a sterol source. Growth ... The surviving alligator tested free of M. alligatoris after 14 weeks, further supporting the researchers suspicions of the new ... Cultures were diluted in broth medium then passed through membrane filters of various pore diameters, yielding similar results ...
... for expression in serum-free suspension. Since QMCF plasmids contain antibiotic resistance gene and are able to stably ... Finally, the process is switched to production phase by changing media temperature to 30 ̊C. construction of protein ... replicate and remain inside dividing cells, a selection and growth of the cell culture takes place. This allows to upscale the ...
Typically this is done in the lab with media containing serum and leukemia inhibitory factor or serum-free media supplements ... The extracted inner cell mass was cultured on fibroblasts treated with mitomycin-c in a medium containing serum and conditioned ... from the donor mother at approximately 76 hours after copulation and cultured them overnight in a medium containing serum. The ... Despite this difference in the cell cycle when compared to ESCs grown in media containing serum these cells have similar ...
Serum-Free media Impact of surfactants in cell culture media on HEK and CHO cell cultivation and transfection Fetal Calf Serum ... Reduced-serum media (commonly 1-5% FBS) Serum-free media (synonymous with Defined media) Protein-free media (no protein but ... using the above definitions this type of media is referred to as serum-free media. Peptide-free, protein-free, chemically ... Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS ...
Elimination of serum from the culture medium for about 24 hours results in the accumulation of cells at the transition between ... Mitotic selection is a drug-free procedure for the selection of mitotic cells from a monolayer undergoing exponential growth. ... Cells can also be resuspended in media and dyed with non-toxic dyes to maintain living cultures. Cells can also be genome ... Cell synchronization is a process by which cells in a culture at different stages of the cell cycle are brought to the same ...
"Que Sera, Sera". Teams who score seven or more times won an "Easy" T-shirt for each member. "We Are the Champions League" (2004 ... The World of Wrestling sketch exploded into British culture in 2005. After destroying his opponent in a public place (e.g. a ... The football-golf link exists due mainly to the amount of free time footballers are allowed in the daytime after training ... Football mass media in the United Kingdom, Sky UK original programming, Sky Sports, Premier League on television, English ...
During this time, patriotic songs such as "Ça sent si bon la France" and "Paris sera Toujours Paris" became popular, and he ... In 1944 when Allied forces freed France, Chevalier was accused of collaboration. The August 28, 1944, issue of Stars and ... Introduction by Robert Osborne, Turner Classic Movies, 11 August 2009 Canadian Culture Online Program. "Expo 67 Man and His ... ISBN 0-8065-0354-8. Wikimedia Commons has media related to Maurice Chevalier. Wikiquote has quotations related to Maurice ...
Museum of Tibetan Culture of China Tibetology Research Center. 1940. Regina A. Corso (29 May 2013). "The Dalai Lama, President ... The 3rd built on his predecessors' fame by becoming Abbot of the two great monasteries of Drepung and Sera. The stage was set ... ISBN 978-0-9724224-4-4. Wikimedia Commons has media related to Dalai Lamas. Wikiquote has quotations related to Dalai Lama. ... said he would not be reborn in a country controlled by the People's Republic of China or any other country which is not free. ...
Emma Baron (2018). Popular High Culture in Italian Media, 1950-1970. Cham, Switzerland: Palgrave Macmillan. p. 55. doi:10.1007/ ... The following year he began a collaboration for Italy's most renowned newspaper, Il Corriere della Sera. At the beginning of ... without the free vitality of the figures in the Trilogy of Life. His final work, Salò o le 120 giornate di Sodoma (Salò, or the ... Mass media people from Bologna, Mass media people from Cremona, Murdered Italian journalists, Nastro d'Argento winners, ...
Wikimedia Commons has media related to Brazil national football team kits. Brazil's first team colors were white with blue ... Football Shirt Culture. 3 January 2018. Archived from the original on 3 July 2021. Retrieved 3 February 2018. "FIFA World Cup ... Brazil defeated England 2-1 in the quarter-finals in Shizuoka, with the winning goal coming from an unexpected free-kick by ... "Dunga completa dois anos na seleção garantindo ser um desafio ganhar o ouro". Globo Esporte (in Portuguese). 24 July 2009. ...
Media related to Human sacrifice at Wikimedia Commons Ashliman, D.L. "Human sacrifice in legends and myths". Lee, Claire (2012- ... Many cultures show traces of prehistoric human sacrifice in their mythologies and religious texts, but ceased the practice ... A judge ruled that those involved in these events had "acted without free will, driven by an irresistible natural force of ... Corriere della Sera (in Italian). Retrieved 10 November 2019. "Boys 'used for human sacrifice'". BBC News. 2005-06-16. Lewan, ...
Media related to Italia dei Valori at Wikimedia Commons Official website (All articles with dead external links, Articles with ... "Pisicchio, l' ideologo di Di Pietro: la nostra base è ex dc". Corriere della Sera. 19 April 2009. "E Di Pietro sfida i "suoi" ... 12 August 2006.[permanent dead link] Carroll, Rory (30 August 2000). "Italy's feuding left gives Berlusconi free run". The ... 2009). "Glossary". Resisting the Tide: Cultures of Opposition Under Berlusconi (2001-06). Continuum International Publishing ...
On 2 June 2010, it was announced Cannavaro would move to UAE League side Al-Ahli on a free transfer after the 2010 World Cup. ... "Cannavaro e la nuova carriera da terzino destro "Se Cuper me lo chiede, gioco anche di punta"". Il Corriere della Sera (in ... Wikimedia Commons has media related to Fabio Cannavaro. Wikiquote has quotations related to Fabio Cannavaro. Official website ... He was relieved of his position on 27 October 2019, and went to corporate culture training, with captain Zheng Zhi the acting ...
By the 12th century, the city was a free comune and it later fought against Frederick I, Holy Roman Emperor as part of the ... Media related to Province of Piacenza at Wikimedia Commons Official website (in Italian) Provincia di Piacenza homepage (in ... Roy Palmer Domenico (2002). The Regions of Italy: A Reference Guide to History and Culture. Greenwood Publishing Group. p. 96. ... ISBN 978-0-313-30733-1. Salvia, Lorenzo (22 October 2012). "Thirty-six Provinces to Go". Corriere della Sera. RCS MediaGroup. " ...
Nutrient media is circulated through the fibers to sustain the cells. During use, plasma is removed from the patients blood. ... Free Full Text. Strain A, Neuberger J (2002). "A bioartificial liver--state of the art". Science. 295 (5557): 1005-9. Bibcode: ... MARS treatment lowers serum urea and creatinine levels improving their clearance, and even favors resolution of hepatorenal ... Li, Jieliang; Pan, Jilun; Zhang, Liguo; Yu, Yaoting (June 2003). "Culture of hepatocytes on fructose-modified chitosan ...
Wayne's serum allows him to act as a superhuman Batman, but he eventually learns that the potion has also given him a split ... Dent takes the law into his own hands twice: once by using his ability to manipulate the legal system to free the Joker, and ... The character has been adapted into numerous forms of media, having been portrayed in live action by Billy Dee Williams in the ... "Public service in popular culture: the administrative discretion of commissioner gordon and harvey dent". International Journal ...
The confusion came from the unfamiliar look of the blood after the serum had separated from the blood cells. Regarding his ... The case was covered extensively by the media in the southern Indiana and Louisville, Kentucky, area, and by national news ... Schlesinger, Richard (2013-01-12). "CBS 48 Hours: Walking Free". CBS News. Kozarovich, Lisa Hurt (2006-06-29). "State of ... Turvey, Brent (2013). Forensic Fraud: Evaluating Law Enforcement and Forensic Science Cultures in the Context of Examiner ...
Sera is noted to have turned away from elven customs and rejects any positive discussion about elven culture or identity, and ... Andy Chalk (January 26, 2015). "Dragon Age: Inquisition tavern music is free to download for a limited time". PC Gamer. ... Characters of Dragon Age: Inquisition Elves in fiction List of fictional tricksters Media portrayal of lesbianism Portal: Video ... Sera is the subject of a song titled "Sera Was Never" composed by Raney Shockne. "Sera Was Never", along with other songs sung ...
Look up ape in Wiktionary, the free dictionary. Ape at Wikipedia's sister projects Definitions from Wiktionary Media from ... Goodman, M. (1964). "Man's place in the phylogeny of the primates as reflected in serum proteins". In Washburn, S. L. (ed.). ... Lawton, G. (2 November 2016). "Every human culture includes cooking - this is how it began". New Scientist. Hoag, Hannah (2 ... McGrew, W. (1992). Chimpanzee Material Culture: Implications for Human Evolution. Dawkins, R. (2005). The Ancestor's Tale (p/b ...
... serum albumin to form bio-conjugates which were highly dispersed in phosphate-buffered saline and could be taken up by cultured ... Wikimedia Commons has media related to Nanocones. (Commons category link is locally defined, Carbon nanoparticles). ... demonstrating a metal-free and user-friendly electrochemical sensing method. Another hydrogen peroxide biosensor was fabricated ... The nanohybrid showed an excellent electrocatalytic activity towards the reduction of chloramphenicol in neutral media, leading ...
Media related to Queen (Snow White) at Wikimedia Commons (CS1: long volume value, CS1 maint: unfit URL, CS1 maint: url-status, ... Jekyll's serum to separate herself from the darkness within her, creating the Evil Queen as a separate individual. In the ... The symbol of an apple has long had traditional associations with enchantment and witchcraft in some European cultures, as in ... Centuries later, she was freed accidentally by Snow White's descendants and has since faced many Justice League superheroes ...
Food portal Wikimedia Commons has media related to Baked beans. List of legume dishes List of toast dishes Pork and beans ... "Health , Experts make flatulence-free bean". BBC News. 25 April 2006. Archived from the original on 31 March 2009. Retrieved 25 ... Baked bean consumption reduces serum cholesterol in hypercholesterolemic adults. Nutrition research (New York, N.Y.) 1 July ... 2004). The Greenwood Encyclopedia of American Regional Cultures: New England. London: Greenwood Press. p. xviii. "Food History ...
Throat cultures done after antibiotic therapy can show if the infection has been removed. These throat swabs, however, are not ... Scarlet fever serum from horses' blood was used in the treatment of children beginning in 1900 and reduced mortality rates ... Springer Science & Business Media. p. 158. ISBN 9781461500537. Archived from the original on 9 January 2017. Ferri, Fred (2018 ... George and Gladys Dick showed that cell-free filtrates could induce the erythematous reaction characteristic of scarlet fever, ...
Splinter explains April had saved them all from the fire and free them into the sewer. The mutagen caused the five of them to ... Fandango Media. Retrieved October 9, 2021. "Teenage Mutant Ninja Turtles (2014)". Metacritic. Red Ventures. Retrieved February ... comedic tone is weighed down by unimaginative pop-culture references and half-witted one-liners". Bill Goodykoontz of The ... plant a device that will flood the city with the virus while Sacks is preparing to convert the mutagen into a healing serum. ...
Maddicks develops a serum based on McCoy's original serum, and tests it on Beast. Hank is also subjected to chemotherapy and ... At the conclusion of the fight, Hank and others are freed from Xavier II's control and allowed to return to their home time, ... The character has also appeared in media adaptations, including animated TV series and feature films. In X2 (2003), Steve Bacic ... cultured, well-traveled, experienced, well-read character so I brought out those parts of his personality which seemed to me to ...
I. Serotypes isolated and serum and middle ear fluid antibody in pneumococcal otitis media". Infection and Immunity. 9 (6): ... Some Christians have objected to the use of cell cultures of some viral vaccines, and the virus of the rubella vaccine, on the ... In the US State of Washington, authorities have given the green light to licensed cannabis dispensaries to offer free joints as ... Injection of horse serum into humans as used in antitoxin can cause hypersensitivity, commonly referred to as serum sickness. ...
Serum PFOA levels in fluorochemical workers are generally below 20,000 ng/mL but have been reported as high as 100,000 ng/mL ... First Look Media Works, Inc. Archived from the original on June 23, 2021. Retrieved May 2, 2020. Halpern M (May 16, 2018). " ... A program licensed and promoted by the Maine Department of Environmental Protection that provided free municipal wastewater ... PFAS with unsaturated bonds are easier to break down: the commercial dechlorination culture KB1 (contains Dehalococcoides) is ...
Solutions - saline, plasma/serum, culture media FSL constructs, when in solution (saline) and in contact, will spontaneously ... In fixed cells or inactive cells (e.g. red cells) stored in serum free media the constructs are retained normally. Liposomes ... but blocking of serum is not required and undiluted serum can be used. A typical procedure is as follows: add serum, incubate, ... Often the mechanism by which an immunoassay is able to overcome this low level activity is to dilute the serum so that the low ...
"Alleged Betrayer Freed". Palm Beach Daily News. 10 March 1936. Right dear in the sight of the Lord is the death of His Saints. ... The song "Que Sera" on the album Silent June by O'Hooley & Tidow was inspired by the execution of Cavell. Louise de Bettignies ... Wikimedia Commons has media related to Edith Cavell. Wikiquote has quotations related to Edith Cavell. Edith Cavell ... on the Rue de la Culture (now Rue Franz Merjay), in Ixelles, Brussels. By 1910, "Miss Cavell 'felt that the profession of ...
West, Amy (August 25, 2022). "Yes, She-Hulk episode 2 features a QR code link to a free comic - here's where to find it". Total ... Fandango Media. Retrieved November 29, 2022. "She-Hulk: Attorney at Law: Season 1". Metacritic. Red Ventures. Retrieved August ... cancel culture, and the "unfair views of women". Bass felt it was "pretty easy" to portray Phelps without any extensive ... former officer in the United Kingdom's Royal Marines who had combined a modified version of the super soldier serum and gamma ...
Those known as 'free settlers' were only permitted to take up land within the approved areas, which from 1826 was confined to ... Qantas was sold in two parcels, one in 1993 and the other in 1995, and the Commonwealth Serum Laboratories was floated in 1994 ... "Media Business Communication timeline since 1861". Caslon. Archived from the original on 29 August 2008. Retrieved 29 September ... law and culture, Australia and Canada followed quite different macroeconomic histories. Australia's GDP per capita was well ...
The bioorthogonality of the reaction has allowed the Cu-free click reaction to be applied within cultured cells, live zebrafish ... Not only is the reaction tolerant of water, but it has been found that the rate increases in aqueous media. Reactions have also ... Although DIFO was extremely reactive in the labeling of cells, it performed poorly in mouse studies due to binding with serum ... Copper-free click chemistry has been adapted to use nitrones as the 1,3-dipole rather than azides and has been used in the ...
Fandango Media. Archived from the original on July 12, 2017. Retrieved August 23, 2022. "The Mummy (2017)". Metacritic. Red ... Nick stops him with a serum that Jekyll invented for this purpose, allowing Nick and Jenny to escape. Ahmanet finds them, ... The crusader zombies kill the Prodigium soldiers and Ahmanet recovers the ruby, combining it with the dagger in order to free ... In BBC World News Culture, Nicholas Barber calls the film "a mish-mash of wildly varying tones and plot strands, from its ...
"Appointment in the order of Arts and Letters - winter 2021". Ministère de la Culture (Ministry of Culture-France). Retrieved 27 ... "Parigi premia il maestro Campanella 'Ufficiale delle Arti e delle Lettere'". Short article published by Corriere della Sera, 11 ... Moreau, Jeanne at "Leonard Jacobson FAIA: Partner, 1980-1992". Pei Cobb Freed & Partners. Archived from the ... Retrieved from "French Embassy media release 04/2001". 22 March 2001. Archived from the ...
History and Practice of Medicine in Non-Western Cultures. Dordrecht: Springer Science & Business Media. p. 35. ISBN 978-0-306- ... ISBN 978-0-313-34101-4. Linton DS (2005). Emil Von Behring: Infectious Disease, Immunology, Serum Therapy. American ... and enrolled their daughters as nuns who provided free health services to the poor. Nursing was a religious role for the nurse ... As tribal culture specialized specific castes, shamans and apothecaries fulfilled the role of healer. The first known dentistry ...
ITSE + A - Blood-free cell culture media supplement. *ITSE Animal-Free® - Blood-free cell culture media supplement ... ITS AF - Blood-free cell culture media supplement. * ... Blood-Free Cell Culture Media. *Cell Culture Components. * ...
... confluence in serum supplemented media culture medium was removed and the cells were washed once with serum free medium (SFM) ... cultured in serum-free and serum-supplemented media. The outcomes were. June 2, 2019. blogadmin ... cultured in serum-free and serum-supplemented media. The outcomes were compared with distributions of these lipids and proteins ... cultured in serum-free and serum-supplemented media. The outcomes were. Home / Uncategorized / Supplementary ...
This webinar shares preliminary data demonstrating the promising performance of a new cell culture media specifically developed ... In this webinar, we will introduce a new xeno-free, serum-free basal media and serum replacement that hold the potential to ... Cell culture media can influence many parameters of your culture such as growth rate, viability and phenotype, but also other ... Gain insights into the potential performance that your T-cell culture could achieve by using a novel cell culture media ...
... mean free of FBS? Or xeno-free, absent of all animal components? Is it defined or chemically defined? The answers can vary. ... What Does Serum-Free Media Mean?. The question was brought up after examining current "serum-free" products on the market. Does ... the definition of serum-free was different across various companies in the cell culture space. So what does serum-free mean to ... What Does Serum-Free Mean for Your Cell Culture Media?. by Dr. Alyssa Master , Apr 2, 2019 ...
animal protein-free culture; high-density culture; human embryonic stem cells; humanized culture medium; plant-derived serum ... Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells. Autoři. KUNOVÁ ... This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free ... This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free ...
In hippocampal cultures, we and others find treatment with A beta protein decreases neuronal survival, but the mechanism of ... In cortical cultures, A beta protein destabilizes calcium homeostasis, but direct neurotoxicity of A beta is not observed. ... Cells, Cultured * Culture Media, Serum-Free * Cyclic AMP / pharmacology * Cyclic GMP / pharmacology ... We have used low-density, serum-free cultures of hippocampal neurons to determine whether the neurotoxicity of A beta protein ...
1 medium. The use of serumfree medium may thus be suitable for some Theileria cell cultures and needs to be evaluated on a ... free, serumfree, protein‐free medium (PFHM II) compared with the control. Out of four South African T. parva strains and a ... free media for the cultivation of Theileria‐transformed cell lines was examined. Three commercial serumfree media (HL‐1, ISF‐1 ... Serumfree in vitro cultivation of Theileria annulata and Theileria parva schizont‐infected lymphocytes. Title: Serumfree in ...
Traditionally, cell cultures, such as VERO, have been used for virus production in bovine serum-containing culture media. ... regulatory agencies suggest avoiding the use of bovine serum in vaccine production. Current serum-free media suitable for VERO- ... We developed a chemically defined, serum-free medium, OptiVERO, that was optimized specifically for VERO cells. VERO cell ... Formulation and production of a blood-free and chemically defined virus production media for VERO cellsexternal icon. Alfano R ...
... a critical aspect is the minimization of interference from medium components and chemical reagents ... Micro-quantitation of lipids in serum-free cell culture media: ... Micro-quantitation of lipids in serum-free cell culture media: ... Fatty acid concentrations in several complex serum-free culture media were quantitated by this method and were very close to ... Micro-quantitation of lipids in serum-free cell culture media: a critical aspect is the minimization of interference from ...
MammoCult™ is the most published commercially available medium for sphere culture from mammary tissue. ... serum-free medium for the culture of mammospheres and tumorspheres. ... MammoCult™ Media for Mammary Cell Culture. Defined, Serum-Free Medium for Mammosphere and Tumorsphere Culture. MammoCult™ is ... Mammary Cell Culture Products View All Products. MammoCult™ Medium (Human) Recommended for:. Culturing mammospheres or ...
The Case for Serum-Free Media. by BPI Contributor February 1, 2003 ... Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags ... Peptides Modulate Growth and Productivity of Mammalian Cell Cultures and Suppress Apoptosis. by BPI Contributor ... Comparing Automated and Manual Cell Counts for Cell Culture Applications. by BPI Contributor ...
Keywords: Human adipose-derived mesenchymal stem cells, BDKRB1 gene, serum-free culture medium, stable cell culture ... Although several kinds of serum-free media are available, a method to confirm whether they contain serum has not been ... A new method to confirm the absence of human and animal serum in mesenchymal stem cell culture media. Int J Med Sci. 16(8):1102 ... A new method to confirm the absence of human and animal serum in mesenchymal stem cell culture media Megumi Ota*, Kentaro ...
Culture in serum-free media. Spheroids do not grow well in serum-free media. Therefore, for the analysis of urea and ... 14175-129) before the addition of serum-free fully defined medium (98.5% UltraDOMA-PF, Complete Protein-Free Medium, LONZA Cat ... To minimize exposure of the spheroids to serum-free media, parallel cultures were set up with an initiation offset of 48 h. ... cells and spheroids were normally grown in standard media but this was temporarily exchanged with serum-free media before ...
Culture of testicular cells in hormone supplemented serum-free medium.. Ann. NY Acad. Sci. 1982; 383: 44-68. *Scopus (90) ... Brain-derived neurotrophic factor increases survival and differentiated functions of rat septal cholinergic neurons in culture. ... Brain-derived neurotrophic factor supports the survival of cultured rat retinal ganglion cells. ...
27.9 μg/mL in cell culture medium (n = 6, dilution factor of 1.2). b) Negative control: Fibrolife serum-free culture medium ( ... Xeno-free culture medium and sub-culture reagents (Lifeline Cell Technology, Carlsbad, USA) were free of animal-derived ... other: Serum-Free culture medium. Details on oral exposure:. 1) Method of administration of test substance A single application ... other: Cultured human dermal fibroblasts in animal product-free culture. Strain:. other: Not Applicable. Sex:. not specified. ...
Replicate cultures were incubated with tannin or control serum free medium and the percent released Cr-51 calculated. Cells ... Lyophilized cotton bract tannin concentrates were dissolved in serum free culture medium. Fourth passage endothelial cells and ... Brief exposure (1 to 30 minutes) to cytotoxic tannin doses produced smaller releases of Cr- 51 into the medium 6 hours later. ... from cells in tissue culture as an index of cell membrane damage. Porcine endothelial cells from the thoracic aorta or ...
The cells were cultured in Dulbeccos Modified Eagle Medium (DMEM; Welgene, Daegu, Korea), containing 10% fetal bovine serum ( ... The secretions of (C) PGD2 and (D) PGE2 were measured in a conditioned medium pre-treated with RDSW for 24 h prior to the ... The secretions of (C) PGD2 and (D) PGE2 were measured in a conditioned medium pre-treated with RDSW for 24 h prior to the ... The cells were cultured in 96-well plates (3 × 104 cells/well) and pre-treated with RDSW for 24 h before 1 μg/mL LPS ...
Cell Culture Process Development job in San Francisco, CA with Mission Barns. Apply Today. ... Experience with serum-free media development and optimization. *Experience with batch and fed-batch cell culture ... Media batching, inoculation, culture, and harvest of bioreactors. *Design, development, and operation of fed-batch and ... Engineer/Scientist - Cell Culture Process Development. Employer. Mission Barns Location. San Francisco, CA. Start date. Oct 5, ...
PC3 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640. Both media were supplemented with 8% fetal bovine serum ... Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing ... Cells were treated with 1 mM LLOME for 10 min and then gently washed twice with pre-warmed culture media; fluorescent images ... Chemicals, silica, or tau fibrils were directly added to cell culture media to induce lysosomal membrane damage with equal ...
Purchase defined serum-free media with GSK3β & Mek1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells ... ESGRO Complete Serum-Free Cell Culture Freezing Medium; 50 mL. The ESGRO Complete Serum-Free cell culture Freezing Medium is ... ESGRO Complete Basal Medium, 100mL. The ESGRO Complete Basal Medium is a defined serum-free medium developed for use as a base ... ESGRO Complete PLUS Clonal Grade Medium, 100ml. The ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium ...
Development of a Serum-Free Chemically Defined Medium for Adherent and Suspension Culture  Logan, Megan (University of ...
Culture of porcine hepatocytes or bile duct epithelial cells by inductive serum-free media - (Peer Reviewed Journal) ... Culture of porcine hepatocytes or bile duct epithelial cells by inductive serum-free media. In Vitro Cellular and Developmental ... Feeder-independent continuous culture of the PICM-19 pig liver stem cell line.. In Vitro Cellular and Developmental Biology. ... Feeder-independent continuous culture of the PICM-19 pig liver stem cell line - (Peer Reviewed Journal) ...
Serum-free media for culturing and serially-passaging of adult human retinal pigment epithelium. ... Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue. ... Expression of melanin-related genes in cultured adult retinal pigment epithelium and uveal melanoma cells. ... Propagation of fetal human RPE cells: preservation of original culture morphology after serial passage. ...
Used for culture of epithelial, endothelial, and granulosa cell types under low serum ... Basal Cell Culture Liquid Media - DMEM and Ham's F-12, 50/50 Mix - ... Serum-free growth may be possible with appropriate protein supplements. *Hams F-12 media is based on Hams F-10 medium with ... Used for culture of epithelial, endothelial, and granulosa cell types under low serum conditions. Corning™ Basal Cell Culture ...
Our high performing HEK media are designed to best fit with the high variability of HEK293 cell lines, the versatility of AAV ... Chemically defined, serum-free, animal component-free, serum-free and hydrolysate-free ... Supports stable growth of suspension cells at high viability in seed train culture ... Little or no adaptation required from other serum-free media. ... Our high performing HEK media are designed to best fit with the ...
1.2.3 Serum-free Medium *1.2.4 Stem Cell Culture Medium *1.3 Market by Application *1.3.1 Global Cell Culture Media Market Size ... Serum-free Medium Product Picture *Figure 5. Stem Cell Culture Medium Product Picture *Figure 6. Global Cell Culture Media ... Classic Medium * Serum-free Medium * Stem Cell Culture Medium * Other Segment by Application. * Biopharmaceutical Production * ... Cell Culture Media Market Size, Share & Trends Analysis Report By Product (Serum-free Media, Classical Media), By Type (Liquid ...
... but not that of IgG or IgA in human-human hybridoma HB4GS cells and human lymphocytes cultured in a serum-free medium. Chitosan ... is ineffective when serum-supplemented medium is used, suggesting that serum may contain a factor(s) that influences IgM ...
E Shape index analysis and F cumulative frequencies of astrocytes cultured in serum-containing or serum-free media and ... Notably, culturing astrocytes in serum free media resulted in 3.43-fold more complex astrocyte as assessed by Shape Index of ... A Cartoon representation of magnetic separation of astrocytes for culture in serum-free, defined media. B Quantitative PCR data ... Astrocytes were maintained in serum-free, defined media (50% Neurobasal media, 50% MEM, 1mM sodium pyruvate, 2mM glutamine, and ...
Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS ... Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 10% ... Incubate cultures at 37°C.. Subcultivation Ratio: 1:6 to 1:12 is recommended.. Medium Renewal: Do not feed cells with growth ... Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described ...
  • In this webinar, we will introduce a new xeno-free, serum-free basal media and serum replacement that hold the potential to significantly improve transduction efficiency, unlocking the possibility of reaching the target clinical dose of gene-edited T cells faster and save on manufacturing cost by reducing the amount of viral vector used in the process. (
  • Or does it mean xeno-free - absent of all animal components? (
  • Neonatal human dermal fibroblast cultures - "Xeno-Free" (HDFn-XF) were obtained commercially as cryopreserved primary cells (Lifeline Cell Technology, Carlsbad, USA). (
  • Xeno-free culture medium and sub-culture reagents (Lifeline Cell Technology, Carlsbad, USA) were free of animal-derived components, providing a fully human cell culture system. (
  • We have used low-density, serum-free cultures of hippocampal neurons to determine whether the neurotoxicity of A beta protein in vitro can be altered by voltage- or ligand-gated calcium channel antagonists or cyclic nucleotides. (
  • This system is capable of propagating mammary stem and progenitor cells in vitro and supports tumorsphere culture of breast cancer stem cells and cell lines. (
  • Two stages of mouse pluripotency have been stabilized to date through in vitro culture. (
  • Here, we cultured primary retinal neurons isolated from adult goat up to 10 days, and established an in vitro model of hyperglycemia for performing morphological and molecular characterization studies. (
  • It stimulates in vitro IgM production but not that of IgG or IgA in human-human hybridoma HB4GS cells and human lymphocytes cultured in a serum-free medium. (
  • It is hypothesized that retinoic acid supplementation in culture medium is able to modulate specific chemical cues especially the matrix metalloproteases (MMPs) to retain quiescent properties of corneal keratocytes previously exposed to serum in vitro. (
  • Bronchial Epithelial Cell Medium Kit is a complete medium designed for optimal growth of normal human bronchial epithelial cells in vitro. (
  • The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human bronchial epithelial cells in vitro. (
  • Palmitic acid-modified bovine serum albumin (PAB) was synthetized and found to own remarkable scavenger receptor-A (SR-A) targeting ability in vitro and in vivo , through which activated macrophages took up PAB nanoparticles (PAB NPs) 9.10 times more than bovine serum albumin nanoparticles (BSA NPs) and PAB NPs could delivery anti-inflammatory drugs celastrol (CLT) to inflamed tissues more effectively than BSA NPs. (
  • FBS is the serum of first choice for culturing cells, tissues and organs in vitro due to its broad applicability: nearly every cell type thrives with it. (
  • All BioWhittaker TM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. (
  • Asbestos -associated damage to cells of the respiratory tract in vitro can be prevented by the simultaneous addition of scavengers of active oxygen species to cultures. (
  • For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. (
  • The normal human-derived keratinocytes were cultured at the air-liquid interface in a chemically defined medium on a permeable polycarbonate insert (surface 0.5 cm2). (
  • They were cultured in chemically defined serum free medium to form a multi-layered epithelium similar to that found in native epidermis. (
  • The Cell lines will utilize a commercially available media platform that is in a suspension culture that is serum-free and, chemically-defined. (
  • The interview covers the involvement of the Graduate School of Medicine in the development of StemFit® for Mesenchymal Stem Cell (MSC), a new chemically defined medium which minimizes the risk of hazardous animal derived ingredients, and how the product has assisted cutting-edge liver regeneration therapy research and efforts towards upcoming clinical trials. (
  • StemFit® for MSC is a chemically defined and human/animal origin free medium, optimized for the isolation and culture of MSCs from bone marrow (BM-MSC), umbilical cord (UC-MSC), and adipose (ADSC) under serum-free, human platelet lysate free conditions. (
  • The fully chemically defined formulation of StemFit® for MSC minimizes lot-to-lot variation and enables highly consistent and superior cell expansion compared to serum containing media. (
  • To overcome these inherent drawbacks of FBS, increasing investigations have been reported to develop animal serum-free and chemically-defined media for MSC expansion. (
  • 1). Both culture media, IS MAB-CD and BalanCD CHO Growth A, contain a combination of common media components, although BalanCD CHO Growth A is a versatile, chemically defined, serum-free medium, more robust than IS MAB-CD in amino acids, vitamins, and trace metal components. (
  • This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency of human ESC. (
  • The interference of derivatizing reagents, extraction solvents and medium additives in the micro-quantitation of lipids was also examined. (
  • The results show that the concentration of fatty acids such as palmitic and stearic acids detected in derivatizing reagents or extraction solvents was in the range of 10-230 μg/L. Tween-80, a surfactant and medium additive, produced nearly 20 FAMEs alone when methylated using a derivatizing agent. (
  • Product bundle consisting of human keratinocytes, optimized media and subculture reagents. (
  • Consumables are further classified as media and reagents. (
  • Akron Biotech Laboratories manufactures the akron biotech serum reagents distributed by Genprice. (
  • He said that cells grown in the absence of animal products such as fetal calf serum are more consistent in terms of quality and perform better than cells grown in these media. (
  • It has been reported that the immunogenicity streptomycin, 10mM HEPES, and 10% heat- of antigen peptide correlates with its binding inactivated fetal calf serum (Complete affinity to MHC class I molecules. (
  • In these cultures, neither omega-conotoxin, nifedipine, verapamil, APV, nor MK-801 altered the survival of neurons exposed to synthetic A beta 1-40. (
  • Brain-derived neurotrophic factor increases survival and differentiated functions of rat septal cholinergic neurons in culture. (
  • Culture of adult neurons of the central nervous system (CNS) can provide a unique model system to explore neurodegenerative diseases. (
  • Typically, primary cultures utilize fetal rodent neurons, but very rarely adult neurons from larger mammals. (
  • We also studied the effect of different glucose concentrations and media composition on the growth and expression of CAMs in cultured retinal neurons. (
  • Hyperglycemia significantly enhances neurite outgrowth in adult retinal neurons in culture. (
  • Collectively, our study demonstrates that metabolic environment markedly affects transcriptional regulation of CAMs in adult retinal neurons in culture. (
  • Analysis of neurons cultured in isolation over time facilitates perturbation of neuron-specific signaling pathways by exposing them to chemical agents, and manipulation of neuronal genes using knock-down or overexpression studies. (
  • Moreover, cultured MB neurons respond to mammalian Reelin by increasing the complexity of their neurite arborization. (
  • Human serum (HS) is the gold standard supplement used in many clinical/manufacturing cell therapy media. (
  • ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. (
  • Human platelet lysate is a growth factor-rich cell culture supplement derived from healthy donor human platelets. (
  • This shift is likely to help human platelet lysate to become an attractive alternative to the serum supplement in area of cell culturing, which is expected to increase demand for human platelet lysate during the forecast period. (
  • The number one free radical fighting supplement you can take is curcumin. (
  • In 2008, we released the second edition of this toolkit in a free online format, which included new additions and updates, many based on the 2007 Expert Committee recommendations published in December 2007 in a supplement to Pediatrics ("Assessment of Child and Adolescent Overweight and Obesity," Pediatrics , December 2007;230 suppl 4:5163-5288). (
  • Cell culture media is generally considered to be the biggest contributor to the cost of cultivating meat, which is primarily the result of the high cost of serum alternatives like recombinant growth factors. (
  • The productivity of recombinant hMAb from the cells cultured in dishes at high cell densities was higher in protein-free medium than in serum-containing medium.True high density culture of the ras-rBHK-IgG cells was done in protein-free medium using the Tecnomouse, which is a novel hollow fiber bioreactor system. (
  • After culture for 30 days in protein-free culture, a total amount of about 14 mg of the recombinant hMAb AE6F4 was obtained, and was shown to be reactive against lung cancer cells in tissues. (
  • In terms of cultured meat, entomoculture has mainly been proposed due to its potential advantages over mammalian cell culture. (
  • Mammalian cells also require a relatively precise measure of carbon dioxide and oxygen to grow - as such, cultures are usually supplemented with an extra 5% carbon dioxide. (
  • It can be used for suspension and monolayer mammalian cultures. (
  • In addition to using MammoCult™, advance your research with our full suite of cancer research tools including specialized cell culture media, cell isolation tools and stem cell detection kits. (
  • Dengue virus isolation and serotyping was carried out in 105 clinically-suspected cases of dengue fever by the mosquito inoculation and cell culture techniques to find a suitable virus isolation technique for a resource-constrained country like Bangladesh and to identify the serotypes responsible for the dengue outbreak in 2000. (
  • This is the first report of dengue virus isolation by mosquito inoculation and C6/36 cell culture in Bangladesh. (
  • In this review, we discuss the latest developments in isolation and culturing approaches of TICs, with focus on colorectal cancer (CRC). (
  • SBI's ExoQuick-TC enables high-throughput, quantitative isolation of exosomes from low volumes (as little as 1 ml) of tissue culture media and certain biofluids (saliva, urine, follicular fluid, and breast milk). (
  • Isolation and Culture of Porcine Thoracic Aortic and Pulmonary Arterial Endothelial Cells Pbrcine endothelial cells from the thoracic aorta and pulmonary artery were isolated by a brief treatment with collagenase as previously described (12). (
  • In this study, the suitability of serum‐free media for the cultivation of Theileria‐transformed cell lines was examined. (
  • The relevance of Theileria cultivation in serum‐free media for applications such as vaccine development requires further examination. (
  • Serum-free and BPE-free cell culture medium for the cultivation of human keratinocytes from the epidermis of juvenile foreskin or adult skin. (
  • Secretions of L. infantum promastigotes were obtained from the cultivation of 6 × 109 promastigotes in serum-free RPMI-1640 medium during a period of 72 h. (
  • Figure 1: Cell density and viability analyzed at selected time points in seven-day cultivation to profile HFN 7.1 cell line culture in selected Irvine Scientific medium. (
  • Cell culture media can influence many parameters of your culture such as growth rate, viability and phenotype, but also other gene-editing critical parameters such as transduction efficiency. (
  • ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β & Mek 1/2 inhibitors to enhance viability of mouse embryonic stem & iPS cells in the absence of serum & feeder cells. (
  • The ESGRO Complete PLUS Clonal Grade Medium is a defined serum-free medium provided with a selective GSK3β inhibitor to enhance viability of mouse embryonic stem cells & increased maintenance of pluripotency in the absence of serum & feeder cells. (
  • b) Percent viability graph of HFN 7.1 cultures. (
  • The proper handling of samples is critical for maintaining the viability of organisms for culture, and a special transport medium is required. (
  • Bio&SELL offers optimized media for the adherent and suspension culture of HEK-293 cells. (
  • For non-adherent cells, dispense 100 µl/well of cells in culture medium to 96-well or 25 µl/well to 384-well poly-D Lysine or other ECM coated plates a couple of hours before the experiment. (
  • All blots were blocked with 5% bovine serum albumin (BSA) in tris-buffered saline with Tween-20 (TBS/T) for 1h at room temperature then rinsed once in TBS/T. Next the blots were incubated with primary antibodies diluted 1:1000 with TBS/T overnight at 4 C. Rabbit polyclonal anti-ACAT1 (Abcam), rabbit polyclonal anti-ACAT2 (Abcam) and rabbit anti- EFEMP-1 (Century Biochemicals) were used as primary antibodies. (
  • typically contain 10%-20% foetal bovine serum (FBS). (
  • The expression of BDKRB1 in Ad-MSCs was induced not only by bovine serum but also by human serum. (
  • Ready to use medium formulated with 15% fetal bovine serum (FBS) and mouse leukemia inhibitory factor (LIF). (
  • Most academic cell culture research uses fetal bovine serum, or FBS, to keep the cells healthy and happy. (
  • Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. (
  • Rising adoption of human platelet lysate in place of fetal bovine serum, funding for life science research and investments, adoption of advanced blood collection and processing technology in emerging countries are the major drivers expected to positively influence the growth of global human platelet lysate market in the coming years. (
  • Fetal Bovine Serum: Are Cell Cultures Cruelty Free? (
  • In this paper the focus is on the production method of fetal bovine serum (FBS) and its welfare and ethical implications. (
  • This is done by adding so called culture medium to the cells, usually combined with fetal bovine serum (serum is blood without cells and clotting factors). (
  • Fetal bovine serum contains many substances which are needed by cultured cells to grow and live properly. (
  • It is being used extensively both in research and (the life science-) industry, making fetal bovine serum a commercially interesting product. (
  • Fetal bovine serum is obtained in those areas of the world where bovines are kept in an extensive manner (e.g. continent of America, Australia, Southern Africa, CIS). (
  • MSCs can be easily expanded in culture media containing fetal bovine serum (FBS) from selected lots. (
  • Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. (
  • The cells ob- tained with this technique were washed free of enzyme with Earle's balanced salt solution, placed into fibronec- tin-coated 96 well Falcon tissue culture plates and cul- tured in Medium 199 supplemented with 10% fetal bovine serum, 100 U/mL penicillin G and 20 ,ug/mL gentamicin. (
  • To load the cells with 51Cr, confluent endothelial cell monolayers in 12 well Co-Star tissue culture plates were incubated with 51Cr sodium chromate in Medium 199 supplemented with 10% fetal bovine serum for 2 hr at 37?C in a fully hum- idified atmosphere of 5% C02:95% air. (
  • Description: A Rabbit Polyclonal antibody against Bovine Serum Albumin from Cow/ Human. (
  • Serum-free cell culture medium for keratinocytes from the epidermis. (
  • Primary Human Keratinocytes isolated in serum-free and BPE-free Keratinocyte Growth Medium 3. (
  • The 3-Dimensional Human Dermal Epithelial Model (EpiDerm™, MatTek, Ashland, MA) is made up of normal human keratinocytes in serum free medium. (
  • Normal neonatal human keratinocytes (HEKn) ( C-001-5C ) were grown in EpiLife medium and in a keratinocyte medium from a leading competitor. (
  • The availability of an animal protein-free medium supplemented with the low-cost VegetaCell reagent expands the repertoire of media for culturing human ESC as well as induced pluripotent stem cells for drug testing and cell replacement therapy. (
  • This medium is intended for cell expansion and growth at clonal density in serum-free conditions and supports a naive/ground pluripotent state. (
  • StemMACS PSC-Brew XF, human is a new generation culturing medium for highly efficient maintenance and expansion of human pluripotent stem cells (hPSC). (
  • Pluripotent stem cells cultured in StemMACS PSC-Brew XF display typical colony morphology (A) and high expression of pluripotency markers (B). (
  • Pluripotent stem cells cultured in StemMACS PSC-Brew XF maintain full pluripotent differentiation potential and can differentiate in all three embryonic germ layers. (
  • Pluripotent stem cells cultured in StemMACS PSC-Brew XF maintain a normal genotype over passages as shown from karyotype analysis (A) and copy number variants (CNV) analysis (B). (
  • The mammosphere culture system involves seeding mammary epithelial cells at low density in an environment that prevents their adherence to a substratum and enables their proliferation in suspension as spherical clusters. (
  • Used for culture of epithelial, endothelial, and granulosa cell types under low serum conditions. (
  • Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells. (
  • Even under the most stringent feeder-free conditions without conditioned medium, human ESC exhibited an undifferentiated morphology, expressed markers of undifferentiated cells, demonstrated high alkaline phosphatase activity and multilineage differentiation and retained a normal karyotype. (
  • The ESGRO Complete Basal Medium is a defined serum-free medium developed for use as a base medium for differentiation studies, for serum free culture of ES cells when used with LIF & BMP. (
  • Confluent C2C12 myoblasts were cultured in differentiation media for 5 days to form mature myotubes. (
  • In this study, we attempted to establish further the potential of this gene as a new marker indicative of the presence of serum in media. (
  • Using a real-time quantitative PCR gene array screening containing 84 functional genes, we verified BDKRB1 as a specific gene upregulated in the presence of serum. (
  • The medium type and presence of serum (FBS) was also found to contribute to the toxicity pattern, with serum providing partial protection. (
  • The Bio&SELL HEK (human embryonic kidney) 293 media serum-free are particularly rich media . (
  • Due to the COVID-19 pandemic, the global Cell Culture Media market size is estimated to be worth US$ 2390 million in 2022 and is forecast to a readjusted size of US$ 4196 million by 2028 with a CAGR of 9.84% during the forecast period 2022-2028. (
  • Vitamin D: an essential adjuvant therapeutic agent in breast cancer - J Int Med Res 2022 Jul - 'Low serum levels of vitamin D have been reported as a risk factor for breast cancer. (
  • After the initial mass-vaccinations, mostly free was completed, from August 2022, the government has permitted conditional market sales of Covishield and Covaxin but the others - Sputnik V and CorBEvax - remain specifically for "restricted emergency use", as per the RTI. (
  • This webinar shares preliminary data demonstrating the promising performance of a new cell culture media specifically developed for T-cell based therapies. (
  • In this webinar, learn about the negative effects of culturing cells in serum and the advantages of switching to serum-free cell culturing. (
  • Free continuing education is offered for this webinar. (
  • Fatty acid concentrations in several complex serum-free culture media were quantitated by this method and were very close to the values indicated in their formulations. (
  • A single application of 8 concentrations of the test substance (n=6) was applied in cell culture medium (dilution factor of 2 for the range finding experiment, 1.5 for the main experiments). (
  • Procedures, which include use of low volumetric ratio of reagent to medium and precipitation of the above surfactants, were developed to minimize background FAMEs to levels which do not significantly affect the quantitation of medium lipids and to diminish the interference caused by Pluronic F-68. (
  • The Akron Biotech Serum reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (
  • A gas chromatography/mass spectrometry (GC/MS) method for the micro-quantitation of lipids, determined as fatty acid methyl esters (FAMEs), in complex serum-free cell culture media was developed. (
  • Next, we assessed bacterial growth and metabolism in the optimized growth medium by using flow cytometry and mass spectrometry. (
  • Culture media consist of basal media, which is a composition of the various nutrients essential to cell growth. (
  • Ota M, Takagaki K, Takaoka S, Tanemura H, Urushihata N. A new method to confirm the absence of human and animal serum in mesenchymal stem cell culture media. (
  • During studies on effect of adipocyte mesenchymal stem cells (Ad-MSCs) on pain using a human pain gene array, we noticed that BDKRB1 gene was constantly upregulated when serum was used in the culture medium. (
  • Human platelet lysate is used in various applications, such as mesenchymal stem cell (MSC) culture, cell-based therapy, therapeutics production, stem cell and others. (
  • Fibronectin (FN) is commonly used in the development of serum-free media for the expansion of mesenchymal stem cells (MSCs). (
  • Mesenchymal stem cells (MSCs) are adult multipotent cells that were initially isolated from bone marrow[ 1 ] and characterized by the fibroblast-like appearance in culture and the capacities to form bone, adipose and cartilage. (
  • The EmbryoMax 2-Mercaptoethanol (100X) is available in a 20 mL format and may be used for routine mouse embryonic stem cell culture applications. (
  • The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium. (
  • Each lot is qualified for mouse embryonic stem (ES) cell culture. (
  • Two pathways exist for the delivery of vitamin A: the extrinsic pathway transports dietary vitamin A in lipoproteins from intestinal enterocytes to tissues, while the intrinsic pathway distributes vitamin A from hepatic stores bound to serum retinol binding protein (RBP). (
  • By day 5, serum-deprived cells grown on transwells secreted Fib3 basally into the matrix. (
  • Compared with human ESC grown in standard culture conditions, human ESC maintained in humanized VegetaCell medium show longer cell cycles and decreased cell death. (
  • Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures. (
  • Cultures grown in EpiLife medium demonstrated population doublings over an extended period compared to cells grown in the competitor's medium. (
  • The organism can be grown in culture using a specific liquid medium developed by Kelly. (
  • The toxic effects of cotton bract tannin on porcine aortic and pulmonary artery endothelial cells were studied using the release of radiolabeled chromium (Cr) from cells in tissue culture as an index of cell membrane damage. (
  • Lyophilized cotton bract tannin concentrates were dissolved in serum free culture medium. (
  • Replicate cultures were incubated with tannin or control serum free medium and the percent released Cr-51 calculated. (
  • Brief exposure (1 to 30 minutes) to cytotoxic tannin doses produced smaller releases of Cr- 51 into the medium 6 hours later. (
  • Immediately before use, stock solutions of tannin JOHNSON, HANSON, AND ROHRBACH were prepared by dissolving the lyophilized tannin in serum-free Medium 199. (
  • The BDKRB1 expression was induced even when Ad-MSCs was cultured with 0.1% serum in the medium. (
  • We concluded that BDKRB1 is a valuable marker to detect traces of both human and animal serum in Ad-MSCs cultures. (
  • When asked, Associate Professor Taro Takami commented "By culturing MSCs using StemFit for MSC, we were able to efficiently grow our cells to a clinical standard and maintain important MSC functions at a high level (lower levels of inflammatory cytokine expression, higher levels of anti-inflammatory cytokine expression and cell senescence markers). (
  • Thrombin treatment enhanced MSCs to express FN at mRNA and protein levels and promoted FN secretion by MSCs, accompanied by potent adherence to the culture plastic. (
  • This product is tissue culture tested including Stem Cells and is available as 500ml sterile filtered unit. (
  • We spiked frozen milk samples from 10 donors to the Rogers Hixon Ontario Human Milk Bank with SARS-CoV-2 to achieve a final concentration of 1 × 10 7 TCID 50 /mL (50% of the tissue culture infectivity dose per mL). (
  • ExoQuick-TC can be used to purify exosomes from a wide variety of tissue culture media 4 , and from certain biofluids such as saliva 1 , urine 2 , follicular fluid 3 , and breast milk 5 . (
  • With ExoQuick-TC, you can obtain high-quality exosomes from tissue culture media and certain biofluids using a protocol that can easily be performed on multiple samples and requires very low volumes of input sample. (
  • After several days of culture, wells containing islands of cells with typical endothelial cell morphology were ex- panded to 35 mm well tissue culture plates. (
  • Chlamydia organisms are obligate intracellular bacteria that must be isolated in tissue culture, mice, or chick embryos. (
  • When the question was asked on Twitter the results leaned towards a cell culture media that contains "no animal or human serum. (
  • Some agreed on no serum at all as their definition with some conditional variations such as serum-derived components being fair game or no animal source as a restriction. (
  • These cells can either be taken from primary cultures (directly from the animal) or from cryopreserved secondary cultures. (
  • Culture media is an instrumental part of cellular agriculture and generating cultured tissue because it is effectively what allows scientists to begin with a relatively small sample of animal stem cells and end up with enough to constitute an entire tissue. (
  • The latter strain also showed an improved generation doubling time of 73.7 ± 21.9 hr in an animal origin‐free, serum‐free, protein‐free medium (PFHM II) compared with the control. (
  • Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (
  • Mission Barns uses cell culture techniques, novel bioprocess engineering, and tissue engineering to grow meat from animal cells for sustainability, public health, and animal welfare reasons. (
  • Cells can be cultured without serum, and for many animal cell culture applications in the biopharmaceutical industry this has been done routinely for years. (
  • In addition, increasing research & development activities and surging demand for animal-free serum media are likely to spur the growth of global human platelet lysate market. (
  • The cell culture industry paradigm tends to shift from bovine to animal-free serum, thus, regional governments are adopting European guidelines for culturing the media for any therapeutic products. (
  • Consequentially, what is considered to be the major alternative technique in animal experimentation - cell culture - itself implies animal welfare aspects as well. (
  • However, the saturated culture media may still contain viable nutrients, which makes the practise wasteful and expensive. (
  • The medium elevates glucose and glutamine levels to deliver nutrients to the tissue-dense hollow-fiber hybridoma cultures. (
  • The culture medium is a kind of various nutrients contained in natural insects, including cereals, beans, egg milk, etc., belonging to a fungus. (
  • Eggs are a powerhouse of disease-fighting nutrients like lutein and zeaxanthin and are also high in protein, and yogurt contains live probiotic cultures to strengthen the digestive tract. (
  • At its tip, there are holes within a spiral channel which pump serums, acids and nutrients into the skin. (
  • Figure 2: Antibody production of HFN 7.1 cultures. (
  • Other risk factors for colonization include prolonged rupture of membranes (greater than 18 hours), intrapartum fever (temperature greater than 100.4°F [38°C]), maternal GBS bacteremia during pregnancy, a previous infant with GBS disease, absent or low serotype-specific serum antibody, or a high GBS maternal disease burden. (
  • Lipids (fatty acids) at a concentration range of 10-100 μg/L are essential components included in most serum-free cell culture medium formulations. (
  • And for cultivated meat companies working with fish species, very few-if any-species-specific commercial media formulations exist. (
  • When it comes to media for culturing fish cells , highly-optimized serum-free formulations haven't yet been reported in the academic literature. (
  • This creates a single cell suspension which can be diluted and divided into culture flasks for propagation. (
  • Add 100 µl/well of cell suspension to 96-well plates or 25 l/well to 384-well plates, allow cells to attach and grow overnight for 16 to 24 hours in cell culture incubators. (
  • 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. (
  • The use of serum‐free medium may thus be suitable for some Theileria cell cultures and needs to be evaluated on a case‐by‐case basis. (
  • Induction of primary cilia formation by serum starvation led to a two-fold reduction in ciliogenesis in fibroblasts derived from pathogenic LRRK2-R1441G knock-in mice. (
  • 24-h serum starvation (SFM) of BMSCs alone or in conjunction with 10 μM Y27632, 5 μM LY294002, 10 μM MPS-Phos, or 0.5 μM Staurosporine. (
  • An analytical method assesses the effects of media condition on cultured antibodies. (
  • The Octet QKe platform is evaluated to establish an analytical method for assessing the effects of media condition on the quality and activity of cultured antibodies. (
  • The affinity and kinetics of monoclonal antibodies produced from a hybridoma cell line cultured in two different types of medium were measured. (
  • In this study, the authors evaluate the Octet QKe platform to establish an analytical method to assess media condition effect on the quality and activity of cultured antibodies by measuring the affinity and kinetics of monoclonal antibodies (mAbs) produced from a hybridoma cell line cultured in two different types of medium. (
  • The Octet QKe platform uses a proprietary biolayer interferometry (BLI) technology to perform real-time, label-free quantitation and kinetic characterization of biomolecular interactions of antibodies, proteins, peptides, and small molecules. (
  • The mouse antihuman fibronectin antibodies from day 7 cultured media were aliquot and stored for kinetic analysis to the matched paired target protein, human fibronectin (catalog no. 1918-FN-02M), R&D Systems. (
  • Serum and nasal wash antibodies associated with resistance to experimental challenge with influenza A wild-type virus. (
  • Sections were incubated over night at 4C with main antibodies in PBS comprising 0.5% Triton X-100 (Sigma, St. Louis, MO, USA) after obstructing with 10% goat serum. (
  • The question was brought up after examining current "serum-free" products on the market. (
  • According to Unisyn, Hybrid Grow simplifies downstream processing and reduces regulatory concerns because it is free of bovine products. (
  • Serum-free alternatives have existed for years, but these products can be even more expensive. (
  • The latest cash infusion will be used to produce low-cost serum-free culture media, build a pilot production line for cultivated meat products and develop safety approvals. (
  • We will strive to bring cell-cultured meat to the table and provide Chinese consumers with healthier, safer and lower carbon meat products," comments co-founder and CEO of Joes Future Food, Dr. Ding Shijie. (
  • However, the problem is most anti-aging serums and products do not address the root of the problem. (
  • To optimize growth, this culture media is generally supplemented with other proteins and growth factors. (
  • Yes, it is ok to use serum free medium, and in that case, there is no need to add any serum to the medium - "serum free medium" contains defined proteins as substitute for real serum. (
  • Proteins secreted by rat decidual cells while growing in culture medium were monitored on gel electrophoresis. (
  • As such, the environment must be "rebalanced"-which is typically accomplished by replacing the entire culture medium as frequently as every 2-3 days. (
  • Additionally, insect cell cultures are typically contaminated with lipid cells called trophocytes or vitellophages, which are precursors to insect egg yolk cells. (
  • While this development is restarted by trypsinisation of 2D cultures (typically after 5 days), recovery continues in 3D cultures up until 15-18 days when changes in growth rate, adenylate kinase, ATP, urea and cholesterol all suggest that spheroids undergo some type of physiological transition. (
  • Screening with primary cells is typically more disease-relevant facilitating better clinical translation of the targets identified, however, these cells are generally more challenging to culture, expand, and manipulate genetically. (
  • Porcine endothelial cells from the thoracic aorta or pulmonary artery were dissociated with collagenase and established as secondary cultures showing endothelial morphology with no evident smooth muscle contamination. (
  • Serum deprivation After reaching confluence in serum supplemented media culture medium was removed and the cells were washed once with serum free medium (SFM) before re-incubating in SFM, DMEMF12 with 1% penicillin/streptomycin (100 units penicillin/100g streptomycin per ml). (
  • These stem cells are then immersed into a culture medium so that they can proliferate. (
  • Leading breast cancer researchers choose MammoCult™ to culture tumorspheres derived from breast cancer stem cells or cell lines. (
  • The ESGRO Complete Serum-Free cell culture Freezing Medium is available in a 50 mL format & has been optimized & validated for Stem cell culture. (
  • Media can be used on feeder dependent and independent pluirpotent stem cell lines. (
  • Toward the end of that nine-month period, SCS should have a far greater understanding of the efficiency with which it can differentiate the cells into muscle cells, and how easy they are to grow and to adapt to SCS' platform technology approach, which is to grow stem cells in a serum-free, feeder-free, automated environment using robotics," he explained. (
  • At the research level, SCS became familiar with Cossu's stem cells and performed basic research with the cells to try and grow them in serum-free culture media. (
  • Serum-free, defined formulation. (
  • Dr. Reza Ovissipour, an assistant professor at Virginia Tech, is embarking on a project to change that by creating an open-source media formulation optimized for growing fish cells. (
  • MammoCult™ is the most published commercially available medium for the culture of mammospheres and tumorspheres. (
  • The most published commercially available medium for sphere culture from mammary tissue. (
  • In hippocampal cultures, we and others find treatment with A beta protein decreases neuronal survival, but the mechanism of neurotoxicity is unknown. (
  • Thus, the toxicity of beta protein in low-density hippocampal cultures was not directly altered either by calcium channel blockers or by the addition of cyclic nucleotides. (
  • Cell death rates of neurones and glia were established in hippocampal cultures following 3h incubations in a HEPES-buffered solution and 24h incubations in full culture medium. (
  • 1. The medium in step B2, is it PBS or cell culture medium? (
  • Furthermore, umbilical cords of full-term neonates had been put through 1% hypoxia tests, hypoxia was connected with increased degrees of NOS1 Gemcitabine HCl inhibitor database in venous endothelial cells from the umbilical cable as well such as lifestyle medium. (
  • For clinical use, cell culture should be done at stable conditions, thus the use of serum should be avoided because of the batch-to-batch variations of serum. (
  • To obtain consistency and eliminate batch to batch variation of your cultured cells. (
  • It's a vicious cycle that the beauty industry has been acclamation, partially, with the proliferation of serum foundations. (
  • The industry needs serum alternatives to address these issues because clinical usage and trials for the cell therapy space are on the rise and the amount of human serum in the world is finite. (
  • Our high performing HEK media are designed to best fit with the high variability of HEK293 cell lines, the versatility of AAV and complexity of viral vector processes from early clinical phases to large commercial scale. (
  • We are happy that it has become possible to culture high-quality cells by this medium so that we can proceed now to a clinical trial. (
  • A system involving the electrical iontophoretic introduction of free silver ions into human wounds for their antibiotic effect has been in clinical use since 1975. (
  • This medium was tested in several culture systems, using human fibroblasts as a feeder layer or Matrigel in a feeder-free culture. (
  • We found that the ras-rBHK-IgG cells could be easily cultured in a protein-free ERDF medium supplemented with iron(III) nitrate, hydroxyethyliminodiacetic acid, and non-protein synthetic attachment factor as well as in a serum-free ERDF medium supplemented with insulin, transferrin, ethanolamine, and sodium selenite. (
  • Immunofluorescence staining revealed that approximately 30-40% of cultured cells expressed neuronal markers. (
  • However, these cultures are of limited value in studying neurodegenerative disease which primarily affects mature and aged neuronal tissue. (
  • A 2017 study was able to achieve serum-free culture of fish cells by adding a growth factor called IGF2, but the cells grew more slowly in the IGF2-containing media than they did in the serum-containing control media. (
  • Multiple media and NGOs estimated that since April 2017, the government detained at least 800,000 and up to possibly more than 2 million Uighurs, ethnic Kazakhs, and members of other Muslim groups, mostly Chinese citizens, in specially built or converted detention facilities in Xinjiang and subjected them to forced disappearance, torture, physical abuse, and prolonged detention without trial because of their religion and ethnicity. (
  • Cette décision nous a été évoquée le mardi 3 janvier 2017 puis confirmée oralement le 5 janvier, par Madame Agnès Evren, Vice-présidente chargée de l'éducation et de la culture au Conseil régional d'Ile-de-France - dont nous sommes un organisme culturel associé. (
  • Three commercial serum‐free media (HL‐1, ISF‐1 and Hybridomed DIF 1000) were evaluated for their ability to support growth of the T. annulata A288 cell line. (
  • 6 In 1955, Eagle carried out the first systematic study of the growth requirements necessary to propagate cells in culture and developed an effective combination of salts, vitamins and serum. (
  • Thus the focus in classical cell culture techniques has been to store cells, cultivate them, and ensure their vigorous growth and propagation. (
  • Our optimized growth medium ensures optimal performance throughout numerous passages. (
  • It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. (
  • The global human platelet lysate market has tremendous growth potential owing to increasing regulatory approvals for vaccine production via cell culture-based and well-developed research centers and institutes. (
  • By using Fusobacterium nucleatum as our study model, we predicted and optimized bacterial growth (media) in silico by using a genome-scale metabolic reconstruction model for a constraint-based modelling approach. (
  • A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required. (
  • The advantages offered by alginate-based material are biocompatibility and support to high density cell culture, which is expected to augment the market growth. (
  • The qualitative measurement, in addition to cell growth and titer production, can be useful to further develop and select essential media components to improve product functionality to a cell line. (
  • Traditionally, the culture growth and biologic titer production have been used exclusively to evaluate the performances of cell lines and the culture environment, such as media development optimization. (
  • Growth culture and titer. (