Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Thyroxine: The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.Chorionic Gonadotropin, beta Subunit, Human: The beta subunit of human CHORIONIC GONADOTROPIN. Its structure is similar to the beta subunit of LUTEINIZING HORMONE, except for the additional 30 amino acids at the carboxy end with the associated carbohydrate residues. HCG-beta is used as a diagnostic marker for early detection of pregnancy, spontaneous abortion (ABORTION, SPONTANEOUS); ECTOPIC PREGNANCY; HYDATIDIFORM MOLE; CHORIOCARCINOMA; or DOWN SYNDROME.Serum: The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.Pregnancy-Associated Plasma Protein-A: A product of the PLACENTA, and DECIDUA, secreted into the maternal circulation during PREGNANCY. It has been identified as an IGF binding protein (IGFBP)-4 protease that proteolyzes IGFBP-4 and thus increases IGF bioavailability. It is found also in human FIBROBLASTS, ovarian FOLLICULAR FLUID, and GRANULOSA CELLS. The enzyme is a heterotetramer of about 500-kDa.Immunoglobulin Light Chains: Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.Culture Media, Serum-Free: CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.Triiodothyronine: A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.Fatty Acids, Nonesterified: FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form.Thyrotropin: A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Thyroxine-Binding Proteins: Blood proteins that bind to THYROID HORMONES such as THYROXINE and transport them throughout the circulatory system.Nuchal Translucency Measurement: A prenatal ultrasonography measurement of the soft tissue behind the fetal neck. Either the translucent area below the skin in the back of the fetal neck (nuchal translucency) or the distance between occipital bone to the outer skin line (nuchal fold) is measured.Thyroid Function Tests: Blood tests used to evaluate the functioning of the thyroid gland.Hypothyroidism: A syndrome that results from abnormally low secretion of THYROID HORMONES from the THYROID GLAND, leading to a decrease in BASAL METABOLIC RATE. In its most severe form, there is accumulation of MUCOPOLYSACCHARIDES in the SKIN and EDEMA, known as MYXEDEMA.Serum Albumin: A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.Culture Media, Conditioned: Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).Time Factors: Elements of limited time intervals, contributing to particular results or situations.Pregnancy Trimester, First: The beginning third of a human PREGNANCY, from the first day of the last normal menstrual period (MENSTRUATION) through the completion of 14 weeks (98 days) of gestation.Paraproteinemias: A group of related diseases characterized by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin.Immunoglobulin lambda-Chains: One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.Radioimmunoassay: Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.Down Syndrome: A chromosome disorder associated either with an extra chromosome 21 or an effective trisomy for chromosome 21. Clinical manifestations include hypotonia, short stature, brachycephaly, upslanting palpebral fissures, epicanthus, Brushfield spots on the iris, protruding tongue, small ears, short, broad hands, fifth finger clinodactyly, Simian crease, and moderate to severe INTELLECTUAL DISABILITY. Cardiac and gastrointestinal malformations, a marked increase in the incidence of LEUKEMIA, and the early onset of ALZHEIMER DISEASE are also associated with this condition. Pathologic features include the development of NEUROFIBRILLARY TANGLES in neurons and the deposition of AMYLOID BETA-PROTEIN, similar to the pathology of ALZHEIMER DISEASE. (Menkes, Textbook of Child Neurology, 5th ed, p213)Thyroid Hormones: Natural hormones secreted by the THYROID GLAND, such as THYROXINE, and their synthetic analogs.Thyroid Gland: A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.Hypoproteinemia: A condition in which total serum protein level is below the normal range. Hypoproteinemia can be caused by protein malabsorption in the gastrointestinal tract, EDEMA, or PROTEINURIA.Blood Protein Electrophoresis: Electrophoresis applied to BLOOD PROTEINS.Hyperthyroxinemia: Abnormally elevated THYROXINE level in the BLOOD.Nigella sativa: A plant genus of the family RANUNCULACEAE that contains alpha-hederin, a triterpene saponin in the seeds, and is the source of black seed oil.Testosterone: A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.Immunoglobulin kappa-Chains: One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.Free Radicals: Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.Euthyroid Sick Syndromes: Conditions of abnormal THYROID HORMONES release in patients with apparently normal THYROID GLAND during severe systemic illness, physical TRAUMA, and psychiatric disturbances. It can be caused by the loss of endogenous hypothalamic input or by exogenous drug effects. The most common abnormality results in low T3 THYROID HORMONE with progressive decrease in THYROXINE; (T4) and TSH. Elevated T4 with normal T3 may be seen in diseases in which THYROXINE-BINDING GLOBULIN synthesis and release are increased.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Hyperthyroidism: Hypersecretion of THYROID HORMONES from the THYROID GLAND. Elevated levels of thyroid hormones increase BASAL METABOLIC RATE.Otitis Media: Inflammation of the MIDDLE EAR including the AUDITORY OSSICLES and the EUSTACHIAN TUBE.Mass Media: Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.Thyrotoxicosis: A hypermetabolic syndrome caused by excess THYROID HORMONES which may come from endogenous or exogenous sources. The endogenous source of hormone may be thyroid HYPERPLASIA; THYROID NEOPLASMS; or hormone-producing extrathyroidal tissue. Thyrotoxicosis is characterized by NERVOUSNESS; TACHYCARDIA; FATIGUE; WEIGHT LOSS; heat intolerance; and excessive SWEATING.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Serum Albumin, Bovine: Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Myxedema: A condition characterized by a dry, waxy type of swelling (EDEMA) with abnormal deposits of MUCOPOLYSACCHARIDES in the SKIN and other tissues. It is caused by a deficiency of THYROID HORMONES. The skin becomes puffy around the eyes and on the cheeks. The face is dull and expressionless with thickened nose and lips.Thyroid Diseases: Pathological processes involving the THYROID GLAND.Iodide Peroxidase: A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC 1.11.1.8.Nephelometry and Turbidimetry: Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.Prenatal Diagnosis: Determination of the nature of a pathological condition or disease in the postimplantation EMBRYO; FETUS; or pregnant female before birth.Multiple Myeloma: A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.Blood: The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.Antithyroid Agents: Agents that are used to treat hyperthyroidism by reducing the excessive production of thyroid hormones.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Insulin: A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Dialysis: A process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Amyloidosis: A group of sporadic, familial and/or inherited, degenerative, and infectious disease processes, linked by the common theme of abnormal protein folding and deposition of AMYLOID. As the amyloid deposits enlarge they displace normal tissue structures, causing disruption of function. Various signs and symptoms depend on the location and size of the deposits.Thyroiditis, Subacute: Spontaneously remitting inflammatory condition of the THYROID GLAND, characterized by FEVER; MUSCLE WEAKNESS; SORE THROAT; severe thyroid PAIN; and an enlarged damaged gland containing GIANT CELLS. The disease frequently follows a viral infection.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Reference Values: The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.Blood Glucose: Glucose in blood.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Methimazole: A thioureylene antithyroid agent that inhibits the formation of thyroid hormones by interfering with the incorporation of iodine into tyrosyl residues of thyroglobulin. This is done by interfering with the oxidation of iodide ion and iodotyrosyl groups through inhibition of the peroxidase enzyme.Thyrotropin-Releasing Hormone: A tripeptide that stimulates the release of THYROTROPIN and PROLACTIN. It is synthesized by the neurons in the PARAVENTRICULAR NUCLEUS of the HYPOTHALAMUS. After being released into the pituitary portal circulation, TRH (was called TRF) stimulates the release of TSH and PRL from the ANTERIOR PITUITARY GLAND.Cholesterol: The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.Prospective Studies: Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Kinetics: The rate dynamics in chemical or physical systems.Lipids: A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)TriglyceridesGraves Disease: A common form of hyperthyroidism with a diffuse hyperplastic GOITER. It is an autoimmune disorder that produces antibodies against the THYROID STIMULATING HORMONE RECEPTOR. These autoantibodies activate the TSH receptor, thereby stimulating the THYROID GLAND and hypersecretion of THYROID HORMONES. These autoantibodies can also affect the eyes (GRAVES OPHTHALMOPATHY) and the skin (Graves dermopathy).Lipid Metabolism: Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Fetal Diseases: Pathophysiological conditions of the FETUS in the UTERUS. Some fetal diseases may be treated with FETAL THERAPIES.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Maternal Age: The age of the mother in PREGNANCY.Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Body Weight: The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.Bacteriological Techniques: Techniques used in studying bacteria.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Thyroiditis, Autoimmune: Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.Tricuspid Valve Insufficiency: Backflow of blood from the RIGHT VENTRICLE into the RIGHT ATRIUM due to imperfect closure of the TRICUSPID VALVE.False Positive Reactions: Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Embryo Culture Techniques: The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Neck: The part of a human or animal body connecting the HEAD to the rest of the body.Hydrocortisone: The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Molecular Weight: The sum of the weight of all the atoms in a molecule.Blood Proteins: Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Adipose Tissue: Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.Carnitine: A constituent of STRIATED MUSCLE and LIVER. It is an amino acid derivative and an essential cofactor for fatty acid metabolism.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Organ Culture Techniques: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)Immunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Human Growth Hormone: A 191-amino acid polypeptide hormone secreted by the human adenohypophysis (PITUITARY GLAND, ANTERIOR), also known as GH or somatotropin. Synthetic growth hormone, termed somatropin, has replaced the natural form in therapeutic usage such as treatment of dwarfism in children with growth hormone deficiency.Blastocyst: A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Fasting: Abstaining from all food.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Social Media: Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.Gestational Age: The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated as the time from the last day of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization.Predictive Value of Tests: In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.Risk Factors: An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.Tumor Markers, Biological: Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.Treatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.Follow-Up Studies: Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Bacterial Proteins: Proteins found in any species of bacterium.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Immune Sera: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.Blood Physiological Phenomena: Physiological processes and properties of the BLOOD.Peptones: Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.Glucose Tolerance Test: A test to determine the ability of an individual to maintain HOMEOSTASIS of BLOOD GLUCOSE. It includes measuring blood glucose levels in a fasting state, and at prescribed intervals before and after oral glucose intake (75 or 100 g) or intravenous infusion (0.5 g/kg).Insulin Resistance: Diminished effectiveness of INSULIN in lowering blood sugar levels: requiring the use of 200 units or more of insulin per day to prevent HYPERGLYCEMIA or KETOSIS.Serum Globulins: All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Fertilization in Vitro: An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Rats, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.Communications Media: The means of interchanging or transmitting and receiving information. Historically the media were written: books, journals, newspapers, and other publications; in the modern age the media include, in addition, radio, television, computers, and information networks.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Free Radical Scavengers: Substances that influence the course of a chemical reaction by ready combination with free radicals. Among other effects, this combining activity protects pancreatic islets against damage by cytokines and prevents myocardial and pulmonary perfusion injuries.Rats, Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Estradiol: The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.Osmolar Concentration: The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).Transferrin: An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.L-Lactate Dehydrogenase: A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Phosphates: Inorganic salts of phosphoric acid.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Serum Response Factor: A MADS domain-containing transcription factor that binds to the SERUM RESPONSE ELEMENT in the promoter-enhancer region of many genes. It is one of the four founder proteins that structurally define the superfamily of MADS DOMAIN PROTEINS.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Contrast Media: Substances used to allow enhanced visualization of tissues.Methods: A series of steps taken in order to conduct research.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Growth Substances: Signal molecules that are involved in the control of cell growth and differentiation.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Embryonic and Fetal Development: Morphological and physiological development of EMBRYOS or FETUSES.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Mice, Inbred C57BLEpithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Biological Assay: A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.Sodium Chloride: A ubiquitous sodium salt that is commonly used to season food.Mice, Inbred BALB CCell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Cycloheximide: Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.TritiumProgesterone: The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Microbial Collagenase: A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC 3.4.24.3.Magnesium: A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.Case-Control Studies: Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Glycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Interleukin-6: A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Potassium: An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.Sheep: Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.

A telomere-independent senescence mechanism is the sole barrier to Syrian hamster cell immortalization. (1/2283)

Reactivation of telomerase and stabilization of telomeres occur simultaneously during human cell immortalization in vitro and the vast majority of human cancers possess high levels of telomerase activity. Telomerase repression in human somatic cells may therefore have evolved as a powerful resistance mechanism against immortalization, clonal evolution and malignant progression. The comparative ease with which rodent cells immortalize in vitro suggests that they have less stringent controls over replicative senescence than human cells. Here, we report that Syrian hamster dermal fibroblasts possess substantial levels of telomerase activity throughout their culture life-span, even after growth arrest in senescence. In our studies, telomerase was also detected in uncultured newborn hamster skin, in several adult tissues, and in cultured fibroblasts induced to enter the post-mitotic state irreversibly by serum withdrawal. Transfection of near-senescent dermal fibroblasts with a selectable plasmid vector expressing the SV40 T-antigen gene resulted in high-frequency single-step immortalization without the crisis typically observed during the immortalization of human cells. Collectively, these data provide an explanation for the increased susceptibility of rodent cells to immortalization (and malignant transformation) compared with their human equivalents, and provide evidence for a novel, growth factor-sensitive, mammalian senescence mechanism unrelated to telomere maintenance.  (+info)

Growth-inhibitory effect of cyclic GMP- and cyclic AMP-dependent vasodilators on rat vascular smooth muscle cells: effect on cell cycle and cyclin expression. (2/2283)

1. The possibility that the antiproliferative effect of cyclic GMP- and cyclic AMP-dependent vasodilators involves an impaired progression of vascular smooth muscle cells (VSMC) through the cell cycle and expression of cyclins, which in association with the cyclin-dependent kinases control the transition between the distinct phases of the cell cycle, was examined. 2. FCS (10%) stimulated the transition of quiescent VSMC from the G0/G1 to the S phase (maximum within 18-24 h and then to the G2/M phase (maximum within 22-28 h). Sodium nitroprusside and 8-Br-cyclic GMP, as well as forskolin and 8-Br-cyclic AMP markedly reduced the percentage of cells in the S phase after FCS stimulation. 3. FCS stimulated the low basal protein expression of cyclin D1 (maximum within 8-24 h) and E (maximum within 8-38 h) and of cyclin A (maximum within 14-30 h). The stimulatory effect of FCS on cyclin D1 and A expression was inhibited, but that of cyclin E was only minimally affected by the vasodilators. 4. FCS increased the low basal level of cyclin D1 mRNA after a lag phase of 2 h and that of cyclin A after 12 h. The vasodilators significantly reduced the FCS-stimulated expression of cyclin D1 and A mRNA. 5. These findings indicate that cyclic GMP- and cyclic AMP-dependent vasodilators inhibit the proliferation of VSMC by preventing the progression of the cell cycle from the G0/G1 into the S phase, an effect which can be attributed to the impaired expression of cyclin D1 and A.  (+info)

Mycophenolate mofetil inhibits rat and human mesangial cell proliferation by guanosine depletion. (3/2283)

BACKGROUND: Mycophenolate mofetil (MMF) is used for immunosuppression after renal transplantation because it reduces lymphocyte proliferation by inhibiting inosine monophosphate dehydrogenase (IMPDH) in lymphocytes and GTP biosynthesis. In the present study we asked if therapeutic concentrations of MMF might interfere with mesangial cell (MC) proliferation which is involved in inflammatory proliferative glomerular diseases. METHODS: Rat and human MCs were growth-arrested by withdrawal of fetal calf serum (FCS) and stimulated by addition of FCS, platelet-derived growth factor (PDGF) or lysophosphatidic acid (LPA). Different concentrations of MMF (0.019-10 microM) were added concomitantly in the presence or absence of guanosine. MC proliferation was determined by [3H]thymidine incorporation. Cell viability was assessed by trypan blue exclusion. Apoptotic nuclei were stained using the Hoechst dye H33258. Cytosolic free Ca2+ concentrations were determined with the fluorescent calcium chelator fura-2-AM. RESULTS: MMF inhibited mitogen-induced rat MC proliferation with an IC50 of 0.45 +/- 0.13 microM. Human MCs proved to be even more sensitive (IC50 0.19 +/- 0.06 microM). Inhibition of MC proliferation was reversible and not accompanied by cellular necrosis or apoptosis. Addition of guanosine prevented the antiproliferative effect of MMF, indicating that inhibition of IMPDH is responsible for decreased MC proliferation. Early signalling events of GTP-binding-protein-coupled receptors, such as changes in intracellular Ca2+ levels were not affected by MMF. CONCLUSIONS: The results show that MMF has a concentration-dependent antiproliferative effect on cultured MCs in the therapeutic range, which might be a rationale for the use of this drug in the treatment of mesangial proliferative glomerulonephritis.  (+info)

Altered expression of the IGF-1 receptor in a tamoxifen-resistant human breast cancer cell line. (4/2283)

The relationship between oestrogen (E2) and insulin-like growth factor-one (IGF-1) was examined in both tamoxifen-sensitive (MCF 7/5-21) and tamoxifen-resistant (MCF 7/5-23) subclones of the MCF 7 cell line. Both subclones were grown in defined, serum-free (SF) medium over a period of 7 days with the addition of E2 or IGF-1 or a combination of both agents. Growth of both MCF 7/5-21 and 7/5-23 cells was stimulated (245% and 350%, respectively) by E2. However, only the growth of MCF 7/5-23 cells was stimulated (266%) by IGF-1. A combination of E2 and IGF-1 significantly enhanced MCF 7/5-21 and 7/5-23 cell growth (581% and 695%, respectively). E2-induced IGF-1 receptor (IGF-1R) levels (as measured by 125I-IGF-1 binding and Northern analyses) in only MCF 7/5-23 cells. This effect was partially inhibited by tamoxifen. In medium containing serum, the growth of only the MCF 7/5-23 cells was significantly inhibited by the IGF-1R monoclonal antibody, alphaIR-3. The detection of E2-induced expression of IGF-2 using RT-PCR was demonstrated in the MCF 7/5-23 cells. These experiments indicate that E2 may sensitize tamoxifen-resistant MCF 7/5-23 cells to the growth stimulatory actions of IGF-2 via up-regulation of the IGF-1R and describes a cell-survival mechanism that may manifest itself as tamoxifen resistance.  (+info)

Protection against necrosis but not apoptosis by heat-stress proteins in vascular smooth muscle cells: evidence for distinct modes of cell death. (5/2283)

We have reported previously that cultured vascular smooth muscle cells (VSMC) isolated from spontaneously hypertensive rats (SHR) show higher proliferation and cell death than normotensive controls. In addition to protecting cells against death, heat stress proteins (HSPs) appear to play a role in cell proliferation. This investigation examines the involvement of HSP72 and HSP27 in altered SHR VSMC proliferation and death. We have performed detailed discriminatory analysis to characterize which type of VSMC death is induced by heat stress (HS) and serum deprivation. Serum deprivation induced apoptosis (caspase-3 cleavage and DNA laddering) and secondary necrosis, the 2 processes being a continuum of each other. In contrast, acute HS (46 degrees C, 30 minutes), which inhibited BN. lx and SHR VSMC proliferation by 2-fold, increased necrosis (by 5-fold and 2-fold, respectively) but not apoptosis. HSP72 and HSP27 expression evoked in VSMC by mild HS (44 degrees C, 15 minutes) 6 hours before acute HS prevented the inhibition of proliferation and induction of necrosis with no effect on serum deprivation-induced or staurosporine-induced apoptosis. This induced expression of HSP72 and HSP27 did not eliminate the higher basal proliferation, apoptosis, and necrosis of SHR VSMC compared with BN.lx VSMC, suggesting that these HSPs are not involved in altered SHR VSMC proliferation and death. Also, although apoptosis and necrosis may be a continuum, in VSMC the 2 processes may be distinguished by HS, in which only necrosis is prevented by prior HSP accumulation. This observation may be of use in designing strategies for cellular protection.  (+info)

O-glycosylation potential of lepidopteran insect cell lines. (6/2283)

The enzyme activities involved in O-glycosylation have been studied in three insect cell lines, Spodoptera frugiperda (Sf-9), Mamestra brassicae (Mb) and Trichoplusia ni (Tn) cultured in two different serum-free media. The structural features of O-glycoproteins in these insect cells were investigated using a panel of lectins and the glycosyltransferase activities involved in O-glycan biosynthesis of insect cells were measured (i.e., UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP-Gal:core-1 beta1, 3-galactosyltransferase, CMP-NeuAc:Galbeta1-3GalNAc alpha2, 3-sialyltransferase, and UDP-Gal:Galbeta1-3GalNAc alpha1, 4-galactosyltransferase activities). First, we show that O-glycosylation potential depends on cell type. All three lepidopteran cell lines express GalNAcalpha-O-Ser/Thr antigen, which is recognized by soy bean agglutinin and reflects high UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase activity. Capillary electrophoresis and mass spectrometry studies revealed the presence of at least two different UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases in these insect cells. Only some O-linked GalNAc residues are further processed by the addition of beta1,3-linked Gal residues to form T-antigen, as shown by the binding of peanut agglutinin. This reflects relative low levels of UDP-Gal:core-1 beta1,3-galactosyltransferase in insect cells, as compared to those observed in mammalian control cells. In addition, we detected strong binding of Bandeiraea simplicifolia lectin-I isolectin B4 to Mamestra brassicae endogenous glycoproteins, which suggests a high activity of a UDP-Gal:Galbeta1-3GalNAc alpha1, 4-galactosyltransferase. This explains the absence of PNA binding to Mamestra brassicae glycoproteins. Furthermore, our results substantiated that there is no sialyltransferase activity and, therefore, no terminal sialic acid production by these cell lines. Finally, we found that the culture medium influences the O-glycosylation potential of each cell line.  (+info)

Flow cytometric cell cycle analysis of cultured porcine fetal fibroblast cells. (7/2283)

Normal development of nuclear transfer embryos is thought to be dependent on transferral of nuclei in G0 or G1 phases of the cell cycle. Therefore, we investigated the cell cycle characteristics of porcine fetal fibroblast cells cultured under a variety of cell cycle-arresting treatments. This was achieved by using flow cytometry to simultaneously measure cellular DNA and protein content, enabling the calculation of percentages of cells in G0, G1, S, and G2+M phases of the cell cycle. Cultures that were serum starved for 5 days contained higher (p < 0.05) percentages of G0+G1 (87.5 +/- 0. 7) and G0 cells alone (48.3 +/- 9.7) compared with rapidly cycling cultures (G0+G1: 74.1 +/- 3.0; G0: 2.8 +/- 1.2). Growth to confluency increased (p < 0.05) G0+G1 percentages (85.1 +/- 2.8) but did not increase G0 percentages (6.0 +/- 5.3) compared to those in cycling cultures. Separate assessment of small-, medium-, and large-sized cells showed that as the cell size decreased from large to small, percentages of cells in G0+G1 and G0 alone increased (p < 0.05). We found 95.2 +/- 0.3% and 72.2 +/- 12.0% of small serum-starved cells in G0+G1 and G0 alone, respectively. Cultures were also treated with cell cycle inhibitors. Treatment with dimethyl sulfoxide (1%) or colchicine (0.5 microM) increased percentages of cells in G0 (24.8 +/- 20.0) or G2+M (37.4 +/- 4.6), respectively. However, cells were only slightly responsive to mimosine treatment. A more complete understanding of the cell cycle of donor cells should lead to improvements in the efficiency of nuclear transfer procedures.  (+info)

Cellular effects of beta-particle delivery on vascular smooth muscle cells and endothelial cells: a dose-response study. (8/2283)

BACKGROUND: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact cellular alterations induced by beta irradiation remain to be elucidated. METHODS AND RESULTS: We investigated in vitro the ability of 32P-labeled oligonucleotides to alter (1) proliferation of human and porcine vascular smooth muscle cells (VSMCs) and human coronary artery endothelial cells (ECs), (2) cell cycle progression, (3) cell viability and apoptosis, (4) cell migration, and (5) cell phenotype and morphological features. beta radiation significantly reduced proliferation of VSMCs (ED50 1.10 Gy) and ECs (ED50 2.15 Gy) in a dose-dependent manner. Exposure to beta emission interfered with cell cycle progression, with induction of G0/G1 arrest in VSMCs, without evidence of cell viability alteration, apoptosis, or ultrastructural changes. This strategy also proved to efficiently inhibit VSMC migration by 80% and induce contractile phenotype appearance, as shown by the predominance of alpha-actin immunostaining in beta-irradiated cells compared with control cells. CONCLUSIONS: 32P-labeled oligonucleotide was highly effective in inhibiting proliferation of both VSMCs and ECs in a dose-dependent fashion, with ECs showing a higher resistance to these effects. beta irradiation-induced G1 arrest was not associated with cytotoxicity and apoptosis, thus demonstrating a potent cytostatic effect of beta-based therapy. This effect, coupled to that on VSMC migration inhibition and the appearance of a contractile phenotype, reinforced the potential of ionizing radiation to prevent neointima formation after angioplasty.  (+info)

title: Galpha12 Protects Vascular Endothelial Cells from Serum Withdrawal-Induced Apoptosis through Regulation of miR-155, doi: 10.3349/ymj.2016.57.1.247, category: Article
|p||strong|Technical Advantage:|/strong|  This proprietary formulation is not available from other suppliers.|br /| Corning Insectagro DS2 Serum-Free/Protein-Free Medium was developed for the growth and maintenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression.  At the optimal temperature range 22-24ºC, DS2 cells grow as a loose monolayer and are readily adaptable to growth in suspension.  Under these conditions, the cells require minimal adaptation to serum-free culture.|br /| - Complete Certificate of Analysis available for each production lot, along with full formulation information|br /| - Produced under the highest industry standards to ensure superior results|/p|
This study points out the interest in HPL as a replacement for FBS in culture media for expansion of human BM MSCs. Thus, HPL-containing media not only preserve their phenotype as well as their differentiation capacity but also shorten culture time by increasing their growth rate. Nevertheless, some differences exist in terms of cytokines produced, suggesting functional differences between MSCs expanded in media supplemented with HPL and FBS. This has to be considered for particular clinical applications.. The possibility to use animal serum-free culture media has been reported in several recent studies by substituting FBS with human-derived supplements such as HPL or human serum [17-25]. In the present study, MSC expansions were performed in three different HPL-supplemented media consisting of BGM (α-MEM) with (M2) or without (M3 and M4) FBS and compared with the standard medium devoid of HPL (M1). M1 (supplemented with 10% FBS + 1 ng/mL FGF2) represents the reference medium for MSC expansion ...
In my experience with serum-free media, cell lines that were normally adherent did not adhere, and did not grow well (Cho1.1, H460, 293T); however, cells that would grow in clumps were more clumpy, but grew slowly (HepG2, H157). I do not know what these changes is growth behavior and apparence have on subsequent experiments, but I would guess it varies with cell lines ...
PromoCells Primary Cancer Culture System is a standardized, animal component-free and serum-free system for the isolation and culture of human primary tumor cells. It supports the long-term culture of cancer cells while maintaining the original tumor properties and heterogeneity with controlled stroma support. Prolonged culture allows for functional selection of malignant cells giving access to an enriched population of primary cancer cells.. An innovative solution for culturing primary cancer cells: ...
Assay for determining the IC50 of a drug! - posted in Cell Biology: HI there, I am confused that most of the researchers to investigate the IC50 of a particular drug will consider to use serum free medium. After obtaining the IC50 concentration, they did not mention whether they were using serum free medium to perform other assay, such as Cell cycle assay...! I am concerning the issue that when to use serum free medium is suitable? Such that if you perform the Cell cyc...
Inclusion body, protein reagents, endotoxin removal, protein clean-up, abundant serum depletion, on-column proteolytic digestion, and urine protein purification.. ...
Affiliation:横浜市立大学,生命ナノシステム科学研究科,教授, Research Field:Cell biology,Fisheries chemistry,Obstetrics and gynecology,Biological pharmacy,広領域, Keywords:浸潤,転移,細胞外マトリックス,癌,human,extracellular proteinase,serum-free culture,serum-independent,cell line,細胞接着因子, # of Research Projects:11, # of Research Products:27
Mesenchymal stem cell (MSC) transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium, but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment. The aim of this stud
Serum-Free media for cell cultures from Biological Industries include chemically-defined, protein-free, and serum-free media formulations for mammalian and stem cell cultures.
Furthermore, in vitro culture is never a physiological environment, so it all depends on how much you want to be able to control your experiment conditions - cells usually do better in a medium that has been supplemented with serum, but culturing them in a serum-free environment allows you to determine the conditions of your experiement much more precisely ...
Defective acute regulation of hepatic glycogen synthase by glucose and insulin, caused by severe insulin deficiency, can be corrected in adult rat hepatocytes in primary culture by inclusion of insulin, triiodothyronine, and cortisol in a chemically defined serum-free culture medium over a 3-day period (Miller, T. B., Jr., Garnache, A. K., Cruz, J., McPherson, R. K., and Wolleben, C. (1986) J. Biol. Chem. 261, 785-790). Using primary cultures of hepatocytes isolated from normal and diabetic rats in the same serum-free chemically defined medium, the present study addresses the effects of cycloheximide and actinomycin D on the chronic actions of insulin, triiodothyronine, and cortisol to facilitate the direct effects of glucose on the short-term activation of glycogen synthase. The short-term presence (1 h) of the protein synthesis blockers had no effect on acute activation of glycogen synthase by glucose in primary hepatocyte cultures from normal rats. Normal cells maintained in the presence of
Cervical explant cultures were first developed by Fink et al.44 for the study of epithelium metaplasia in vitro. They consisted of large tissue explants (5 × 5 × 2 mm or 5 × 10 × 3 mm) cultured on a thin slab of agarose-gelled serum-free Eagles Basal Medium on top of a stainless steel supporting grid. This culture method was successfully adapted to the study of HSV-2 and HSV-1 infection in vitro and was shown to support the replication of these two human herpesviruses.45 OBrien et al.46 modified this method for the study of the production of glycoprotein from normal and malignant cervical explants of smaller size (5 mm3) cultured either fully immersed in serum-free culture medium or maintained at the air-liquid interface, supported only by a stainless steel grid mesh. On the basis of glycoprotein production, the authors concluded that the grid technique was superior to the immersion culture. It was this grid technique that was adapted to the study of HIV-1 infection in human cervical ...
In this study, we characterized signaling cascades activated on direct contact of leukemic cells with bone marrow-derived stromal cells. Our findings indicate stroma-induced activation of ILK with phosphorylation of Akt and GSK3β. Because p-GSK3βSer9 is a known cellular target of ILK ( 7), these results are consistent with evidence of ILK activation induced by growth factors or engagement of the integrins by stroma. We further showed that the nuclear accumulation of β-catenin, a downstream GSK3β target, was induced in NB4 cells by coculture with MSCs and that this accumulation was abrogated when the PI3K/ILK pathway was inhibited. In contrast, the GSK3 inhibitor BIO, which prevented the serum withdrawal-induced apoptosis of NB4 cells, stimulated the translocation of β-catenin to the nucleus in cells growing without stromal support. Altogether, these findings provide evidence that stroma-induced ILK activation results in GSK3 inhibition and up-regulation of nuclear β-catenin consistent with ...
Wnt5a Does Not Support Hematopoiesis in Stroma-Free, Serum-Free Cultures. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Staurosporine inhibits apoptotic blebbing in PC12 and COS-7 cells. Serum removal and z-VAD-FMK treatment were performed as described in the legend to Fig. 1.
Rat (Adipose Derived) Stem Cell Media (Serum Free Medium or Medium with Serum). This product is tissue tested including Stem Cells and is available as 500ml sterile filtered unit. The product is also available as a pack of 6, 500ml unit sizes ...
Human Mesenchymal (Liver) Stem Cell Media (Serum Free Medium or Medium with Serum). This product is tissue tested including Stem Cells and is available as 500ml sterile filtered unit. The product is also available as a pack of 6, 500ml unit sizes ...
BioAssay record AID 89632 submitted by ChEMBL: Cell viability of serum-deprived Jurkat cells were determined, 24 hours after incubation with 100 uM concentration of the compound, by MTT-assay.
EBV infection in stomach epithelial cells in vitro invariably showed the latency I type of EBV-latent gene expression similar to the neoplastic cells of EBV-associated gastric carcinoma in vivo ( 22). EBV-infected gastric carcinoma cells expressed EBNA1, EBERs, LMP2A, and BARF0, but not EBNA2 or LMP1. In this experimental condition, we showed that three of four gastric carcinoma cells of latency I EBV infection showed resistance to serum deprivation-induced apoptosis, although the growth rate and cell mobility analyses did not show any differences. Serum deprivation, mimicking the microenvironment of cancer tissue in vivo, is a strong inducer of apoptosis through the intrinsic pathway. Interestingly, a lower rate of apoptosis has also been well documented in EBV-associated gastric carcinoma in vivo ( 25); therefore, the mechanisms of resistance to apoptosis may be important in this type of gastric carcinoma and should be further investigated in relation to EBV infection.. Subsequent ...
Recently, cell lifestyle systems producing hepatitis C virus particles (HCVcc) were developed. we developed serum-free culture systems producing high-titer single-density sf-HCVcc, showing similar biological properties as HCVcc. This methodology has the potential to advance HCV vaccine development and to facilitate biophysical studies of HCV. within the family. Due to a high degree of genetic heterogeneity, HCV has been categorized in 6 essential genotypes and several subtypes epidemiologically, differing in around 30% and 20% of their nucleotide and amino Vorinostat acidity sequence, [3 respectively,4]. Genotypes display important biological and clinical variations [5C10]. Serotypes Rabbit Polyclonal to 5-HT-2C. never have been defined; nevertheless, different genotypes and subtypes display differential level of sensitivity to neutralizing antibodies within sera of chronically contaminated patients also to monoclonal neutralizing antibodies with restorative potential [6,11C14]. The 9.6 kb HCV ...
The HL-60 (Human promyelocytic leukemia cells) cell line has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at MD Anderson Cancer Center. HL-60 cells are predominantly a neutrophilic promyelocyte (precursor). Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this line, differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide (DMSO), or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 ...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 30 Nov 2017. Apply now!. ...
The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturin
For typical Western blotting experiments, (e.g., Fig. 2J-L), cells were plated at 200,000 cells per well of a 12-well plate in complete Dulbeccos modified Eagles medium (DMEM)/F12 media containing 10% serum. The next day, cells were treated as indicated. For Western blotting involving production of cytokines, ARPE-19 cells were plated as described above followed by gradual serum removal over the course of a week until the serum was removed completely followed by indicated treatments. At appropriate time points, ARPE-19 cells were lysed in-plate with either radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris, pH 7.4, 150 mM NaCl2, 1% Triton X-100 [vol/vol], 0.1% SDS [wt/vol], 0.5% sodium deoxycholate [wt/vol]) supplemented with Halt Protease and Phosphatase inhibitors (Pierce, Rockford, IL, USA) and benzonase (Sigma-Aldrich Corp.) or a buffer containing 50 mM HEPES pH 8, 10 mM KCl, 2 mM MgCl2, and 1.0% SDS also supplemented with benzonase. Cells were lysed at RT for approximately 1 to 2 ...
Application: ELISA systems, In vitro neutralization, Immunohistochemistry and Western blot analysis. Clone: DB-14 Isotype: Mouse IgG|sub|2a Production: In vitro using serum free medium. Purification: Ion exchange chromatography. Packaging: Lyophilized and vacuum-packed. Contents: 0.5 mg/vial Buffer: Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose. Specificity: Neutralizes both natural and recombinant rat IFN-gamma in vitro. The antibody does not cross-react with mouse and human IFN-gamma and does not bind to rat IFN-alpha and -beta.
The ultrastructure of bovine morulae and blastocysts developed from in vitro-matured and -fertilized oocytes in a serum-supplemented medium was compared with that of morulae and blastocysts collected
A. PRIME-XV MSC Expansion SFM outperforms leading competitors in cell expansion studies while maintaining MSC gross morphology, cell surface expression markers, multipotency potential and immune modulation abilities. Additionally, PRIME-XV MSC Expansion SFM has been tested to support optimal cell expansion of different sources of MSCs, including bone marrow-derived and adipose-derived MSCs. Little to no adaptation is required when shifting from a serum-containing medium to our medium. PRIME-XV MSC Expansion SFM is ready-to-use with no additional growth supplements required and is supplied in a convenient one 250mL complete component. ...
Cornings comprehensive line of standard and custom media helps create an optimal environment for all stages of cell culture growth and scaling.
Cells wont adhere to the coverslip without serum, so grow them first for 24 hours in regular medium.. Remove the medium, replace with serum-free medium. Fix the coverslips 24 hours later.. ...
Effects of murrayafoline A on the PDGF-BB-stimulated activation of PDGF-Rβ, PLCγ1, Akt, ERK1/2, and STAT3. Quiescent VSMCs cultured in serum-free medium were
Easy to read snippets and explanations on dermatology research. Learn about the role of diet in acne and the debate over paper or electronic prescriptions.
Two purified serum protein fractions, fetuin and serum albumin, will replace whole or dialyzed serum in supporting the growth of single S3 HeLa cells in an otherwise chemically defined nutrient solution.. In the serum-free medium, single S3 cells will form macroscopic colonies with essentially 100 per cent efficiency.. The generation time of S3 cells in the serum-free medium is approximately 50 per cent greater than that observed in an optimal, serum-containing medium.. All components of the serum-free medium are available commercially, except fetuin, which can easily be prepared in substantial quantities.. The problem of the purity of the protein preparations and of their possible roles in promoting cell growth is discussed.. ...
Abstract: From the emerging field of cellular therapy, there is an increasing demand for new media to provide optimal expansion of cells in culture. Hematopoietic stem cells have complex growth requirements and serum free media include over 50 components at concentrations from near zero to 7 g/L. Conventionally, media are developed empirically, either by depletion analysis or component addition to determine if culture productivity can be increased. This optimization process consumes valuable time and labour. We are investigating medium optimization through monitoring the gene expression profile of cells in culture based on the hypothesis that cells experiencing a particular limitation will exhibit a characteristic gene expression profile. We have analyzed gene expression of human TF-1 cells under nutritional stress and literature data on yeast under amino acid limitations. The expression levels of genes in pathways relevant to these stresses were monitored at several time points following ...
The monoclonal antibody to human TNF-alpha (clone B-F7) recognizes both native and recombinant human TNF-alpha. The B-F7 antibody is suitable for ELISA and ELISPOT assays. Produced In vitro using serum free medium.
Make use of our cell line adaptation service and we adapt your adherently growing cell line to serum-free growth in suspension - For scalable biologi…
By conducting the entire media development project in our laboratories, we are able to deliver the most improvement in the shortest amount of time. Once we receive the primary or stem cells, we will perform all of the research necessary to discover the optimal media for our customers process. Beginning with either a customer-owned formula or with our own proprietary media, we will develop growth or differentiation media designed explicitly to meet each customers specific process requirements such as serum-free, xeno-free, animal-component-free, growth rate, differentiation efficiencies, etc.. ...
We established a serum-free organ culture system of isolated single vibrissa rudiments taken from embryonic day 13 mice. This… Expand ...
The present study demonstrates that PPARγ-activating ARBs induce adiponectin protein expression at a post-transcriptional level, independently of their AT1R-blocking properties. In addition, AT2R activation resulted in adiponectin upregulation. The PPARγ-activating ARB irbesartan improved parameters of insulin sensitivity in obese Zucker rats, which was associated with the prevention of adiponectin serum depletion.. We and others could recently demonstrate that a subset of ARBs including irbesartan has the potential to activate the insulin-sensitizing nuclear hormone receptor PPARγ, completely independent from their AT1R blocking properties.8,10 PPARγ activation has been shown to stimulate adiponectin expression in adipocytes and to upregulate adiponectin plasma levels in animals and humans.6,11 In the present study, pharmacological antagonism of PPARγ completely blocked irbesartan-induced adiponectin expression in vitro. In addition, adiponectin expression in adipocytes and fat tissue was ...
The derivation of human macrophages from peripheral blood monocytes remains a convenient method for the study of macrophage biology. However, for macrophage differentiation, a significant proportion of development has occurred prior to the monocyte stage; monocyte subsets also have varying potential for differentiation. Differentiation of macrophages from a less mature precursor, such as CD34+ haematopoietic stem cells, can further inform with regard to the development of macrophage-lineage cells. CD34+ cells were cultured in serum-free medium containing Flt3L, SCF, IL-3, IL-6 and M-CSF. Using differing combinations of growth factors, the effect on cell proliferation and differentiation to adherent macrophage-like cells was determined. The proliferative response of CD34+ cells to M-CSF was determined during the initial phase of cell culture. Thirteen combinations of SCF, IL-3, IL-6 and M-CSF were then compared to determine the optimum combination for proliferation. Adherence was used to isolate mature
Cardiomyocytes from human stem cells have applications in regenerative medicine and can provide models for heart disease and toxicity screening. Soluble components of the culture system such as growth factors within serum and insoluble components such as the substrate on which cells adhere to are important variables controlling the biological activity of cells. Using a combinatorial materials approach we develop a synthetic, chemically defined cellular niche for the support of functional cardiomyocytes derived from human embryonic stem cells (hESC-CMs) in a serum-free fully defined culture system. Almost 700 polymers were synthesized and evaluated for their utility as growth substrates. From this group, 20 polymers were identified that supported cardiomyocyte adhesion and spreading. The most promising 3 polymers were scaled up for extended culture of hESC-CMs for 15 days and were characterized using patch clamp electrophysiology and myofibril analysis to find that functional and structural ...
Defined serum-free media inventor and manufacturer ( SYN H, SPE IV ), and characterized human primary cells manufacturer (HUVEC, EPC, HUAEC, MSC, CD34, CD133), ABCell-Bio succeeded in convincing well known firms and institutes, involved in very different fields : vascular biology, hematopoietic, tissue regeneration and cellular therapy. ABCell-Bio proposes in these fields, high standard cell and media kits and thus able to meet the more and more accurate biologists’ needs.
Defined serum-free media inventor and manufacturer ( SYN H, SPE IV ), and characterized human primary cells manufacturer (HUVEC, EPC, HUAEC, MSC, CD34, CD133), ABCell-Bio succeeded in convincing well known firms and institutes, involved in very different fields : vascular biology, hematopoietic, tissue regeneration and cellular therapy. ABCell-Bio proposes in these fields, high standard cell and media kits and thus able to meet the more and more accurate biologists’ needs.
Lewis lung adenocarcinoma or was a little effective in inhibiting DNA synthesis on Lewis lung cells and L1210 leukemia cells ( Carchman et al. 1976 ). Does Cannabis Oil Have Thc after that to our knowledge the only report that has studied CBD was the work of Jacobsson et al. ( 2000 ) demonstrating that in C6 rat glioma cells CBD had a modest effect only evident after 6 days of incubation with the drug and only in a serum-free condition. At that time no further investigation or discussion was put forward about the ability of this compound to alter the proliferation rate of glioma cells. In our study we demonstrated that CBD caused a concentration-related inhibition of the glioma cell viability under serum-free conditions to exclude any interaction with the reported direct interaction between serum proteins such as albumin and cannabinoids ( Zheng et al.. S.E.M of at least three experiments. ?? p , 0.01; ??? p , 0.001 versus control (C) Students t test. Mechanism of Action of CBD. Since ceramide ...
In the first experiment, cells growing in medium supplemented with the two combinations containing a trace element in common died at 2.5 % serum, while for the remaining combinations cell death occurred at a later stage, 0.625 % serum. Cell death was attributed to problems with the procedure of adaptation used in the first experiment, which were identified and corrected in the second experiment. The problems found and the procedure modifications implemented included the use of higher initial cell concentrations to allow the survival of an increased number of cells during the process; avoiding procedures that can be harsh to the cells, such as centrifugation and the use of enzymes (i.e. trypsin) due to a higher sensitivity of cells during adaptation; and allowing enough time in each step of the process for a complete cell adaptation.. After these modifications, in the second experiment, it was possible to observe that cells required a long time to adapt to each level of serum concentration, ...
A human monocytic cell line, THP-1-S, was cultured in a serum-free medium. The effect of the culture supernatant of THP-1-S on the cytotoxicity of rTNF-alpha to three kinds of cell lines and the binding of rTNF to its ...
ATCC offers a variety of media formulations for use in serum-free or low-serum culture conditions to support the growth of stem cells or primary cells.
ATCC offers a variety of media formulations for use in serum-free or low-serum culture conditions to support the growth of stem cells or primary cells.
We investigated the expression of the alpha- and beta-subunits of the lysosomal enzyme beta-N-acetylhexosaminidase in the BV-2 microglial cell line under different culture conditions. Beta-N-acetylhexosaminidase from BV-2 microglia cells was separated into its constituent isoenzymes on diethylaminoethyl (DEAE) cellulose, and its activity was monitored with 4-methylumbelliferyl-beta-N-acetylglucosamine and 4-methylumbelliferyl-beta-N-acetylglucosamine-6-sulphate substrates. Forms corresponding to the mouse isoenzymes A and B were present in the cells incubated in serum-supplemented medium as well as in serum-free medium. Lipopolysaccharide, a well-known activator of microglia in vitro, added to the BV-2 cells in serum-supplemented medium induced a decrease in the specific enzymatic activity determined with the 4-methylumbelliferyl-beta-N-acetylglucosamine substrate. Lipopolysaccharide had no effect on hexosaminidase isoenzyme pattern of BV-2 cells in serum-supplemented medium. The level of ...
Purpose: : To investigate possible mechanism involved in the de-differentiation of human corneal epithelial cells in a defined low calcium and serum free medium Methods: : Human corneal epithelial cells were cultivated from fresh limbal tissue explants in two conditions: high calcium serum-containing SHEM and a defined, low calcium and serum free medium (CnT-20). After 2 weeks, they were passaged and seeded at two densities, low and high, in CnT-20 medium. Colony forming efficiency without any feeder layer was quantified in low density condition. Subsequent passages were performed for cells seeded at high density. Gene expression was analyzed by reverse transcription and real time PCR with TaqMan primers and probes. Protein localization was determined by immunofluorescent staining on corneoscleral tissue cryosections and in cell cultures. Results: : Corneal epithelial cells harvested from explant cultures in the low calcium serum-free defined medium CnT-20 generated 2 fold colony forming ...
A generic research platform with 2-dimensional (2D) cell culture technology, a 3-dimensional (3D) in vitro tissue model, and a scaled-down cell culture and imaging system in between, was utilized to address the problematic issues associated with the use of serum in skin tissue engineering. Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium were compared and analyzed via the platform. It was demonstrated that serum depletion had significant influence on the attachment of HaCat cells onto tissue culture plastic (TCP), porous substrates and cellulosic scaffolds, which was further enhanced by the pre-seeded HDFs. The complex structures formed by the HDFs colonized within the porous substrates and scaffolds not only prevented the seeded HaCat cells from filtering through the open pores, but also acted as cellular substrates for HaCat cells to attach onto. When mono-cultured on TCP, both HDFs and HaCat cells were less proliferative
NO loss of potency whatsoever . Protein determination was performed using BCA protein assay kit (Pierce Rockford IL) following the manufacturers instructions and the absorbance of protein was determined at 580 nm wavelength. Sample cocktail buffer (0. C for 5 minutes. The pre-prepared polyacrylamide gels (varied depending on the size of protein of interest refer to table 4.. A1 1A2 2A6 2E1 3A4 and human epoxide hydrolase (Crespi et al 1991). CYP 1A inhibitor) and 3-amino124-triazole (CYP 2E1 inhibitor) were used to assess the possible metabolic activity in mediating the MSE and MIT toxicity in MCL-5 cells. The results shown in fig. Alphanaphtoflavone (bar graph D) also showed some marginal difference in inhibiting the MSE toxicity. Cytotoxicity was apparently unaffected by ketoconazole.. Serum free media was added to respective wells and treated with various Top Kratom Vendors 2012 Newbury concentrations of MSE. Triplicate wells of 10% FBS media for control group were also added for comparison. ...
Application: ELISA and ELISPOT systems and Flow cytometry. Clone: GB-11. Isotype: Mouse IgG|sub|1. Production: In vitro using serum free medium. Purification: Ion exchange chromatography. Packaging: Lyophilized and vacuum-packed. Contents: 0.5 mg/vial. Buffer: Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose. Specificity: Binds both natural and recombinant human Granzyme B in vitro.
Differentiated osteoclasts were generated and then cultured for 48 h in serum-free medium supplemented with 20 ng/ml M-CSF and 2 ng/ml RANKL. Conditioned medium was harvested, centrifuged. to remove cells and debris, and 600 μl/well was added to 24-well plates. Serum-free medium and medium containing 10% FBS, were supplemented with M-CSF and RANKL, and used as negative and positive controls, respectively. Wortmannin order Prior to the chemotaxis assay, γδ T cells were activated for 12 h with 100 U/ml rhIL-2. γδ T cells were then re-suspended in serum-free medium at 106 cells/ml and 80 μl of cell suspension was added into Transwell inserts (8 μm pore size). γδ T cells were incubated for 4 h at 37 °C to allow migration through the http://www.selleckchem.com/products/Staurosporine.html Transwell membrane. Cells that had migrated into the bottom chamber were harvested and quantified using flow cytometric analysis on an LSRII flow cytometer (BD Biosciences) by counting an equivalent volume ...
Serum-free medium for the culture and expansion of hematopoietic cells isolated from human cord blood, bone marrow, or mobilized peripheral blood.
Over 30 years of experience in developing and manufacturing a diverse cell culture media, including Sera, FBS, Stem Cell Products including NutriStem hPSC and MSC media, CryoStem Freezing Media, and other serum-free, xeno-free media solutions. Products also include human cytogenetic analysis kits, lab sterility (Aquaguard and Pharmacidal), and more.
diluted in serum-free non-CO2 medium 1 mL/group 100 nm working concentration stock is 1 mM in DMSO Mix 1 µL in 10 mL Aliquot ~1.2 mL/group Either view live, or fix in 3.2% formaldehyde (in PBS) NO DETERGENT ...
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TY - CHAP. T1 - Reconstruction of normal and pathological human epidermis on polycarbonate filter. AU - De Vuyst, Evelyne. AU - Charlier, Céline. AU - Giltaire, Séverine. AU - De Glas, Valérie. AU - De Rouvroit, Catherine Lambert. AU - Poumay, Yves. N1 - ISSN 1064-3745. PY - 2014. Y1 - 2014. N2 - This chapter provides methods suitable for the culture of primary human keratinocytes in serum-free culture conditions, starting from very small skin biopsies. It also explains procedures required for reconstruction of a stratified epidermis on polycarbonate filter, starting from keratinocytes cultured in serum-free conditions. Tissues reconstructed according to this method have been proven suitable for characterization of epidermal morphogenesis and for in vitro studies of the epidermal barrier. Utilization of the same method for successful isolation of keratinocytes from a patient suffering from Dariers disease and the reconstruction of a pathological epidermis which displays the same histological ...
Dipartimento di Istologia ed Embriologia Medica, Universita La Sapienza, Rome, Italy. Terminal differentiation of myogenic cells has long been known to be positively regulated by insulin-like growth factors (IGFs). Arg8-vasopressin (AVP) has been recently reported to potently induce myogenic differentiation. In the present study, the effects and the mechanisms of action of AVP and IGFs on myogenic cells have been investigated under conditions allowing growth and differentiation of myogenic cells in a simple serum-free medium. Under these conditions, L6 and L5 myogenic cells slowly proliferate and do not undergo differentiation (less than 1% fusion up to 7 days). AVP rapidly (2-3 days) and dose-dependently induces the formation of multinucleated myotubes. Creatine kinase activity and myosin accumulation are strongly up-regulated by AVP. Insulin or IGF-I or IGF-II, at concentrations that cause extensive differentiation in serum-containing medium, induces a modest degree of differentiation in ...
With the goal of deriving clinically safe USSCs, we aimed to culture established USSCs in the serum- and animal component-free medium, USSC growth mediumACF. We observe that USSCs continue to proliferate in USSC growth mediumACF, but after one to three passages, the cells aggregate and grow in suspension as spheres. We show that spheres can be dissociated and can continue to grow for five passages, as long as they are dissociated before the sphere becomes cystic. We also show the spheres can revert to monolayer growth when provided with extracellular matrix support or when plated in medium containing fetal calf serum. Cells passaged in USCC growth mediumACF maintained their gene expression profile as judged by Sox2, Brachyury, Pax6 and Gata6 expression. Cells also maintained their capacity to form bone-like Alizarin red positive cells, SPC positive epithelial cells and β-tubulin III positive neuronal cells after directed differentiation. This is the first report of a serum-free medium that ...
Protein coronas around silver nanocubes were quantified in serum-containing media using localized surface plasmon resonances. Both soft and hard coronas showed exposure-time and concentration-dependent changes in protein surface density with time-dependent hardening. We observed spatially dependent kinetics of the corona-formation at cube edges/corners versus facets at short incubation times, where the polymer stabilization agent delayed corona hardening. The soft corona contained more protein than the hard corona at all time-points (8-fold difference with 10% serum conditions ...
Mediatech Inc., a Corning Subsidiary, is a manufacturer of sterile solutions ranging from cell culture media, molecular biology reagents, bioprocessing containment systems, and custom manufacturing.
Effects of 6-thioguanine on PTHrP content in the media of cultured MDA-MB-231 cells plated onto 48-well plates and grown to near confluence. Cells were washed and treated with serum-free media containing 6-thioguanine in the indicated concentrations for 48 hours. PTHrP concentrations in conditioned media were corrected for cell number. The detection limit of the assay is ∼0.5 pM/L ...
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
هدف: هدف از این مطالعه تجربی بررسی تأثیر هورمون‌های تخمدانی بر بیان ژن‌های دخیل در لانه گزینی در سلول‌های استرومایی آندومتر انسان در محیط کشت بود. مواد و روش‌ها: پس از تأیید نرمالیتی بافت‌های آندومتر حاصل از هیسترسکوپی با رنگ‌آمیزی هماتوکسیلین و ائوزین، سلول‌های آندومتر به کمک کلاژناز تیپ 1 جداسازی و از فیلترهایی به ابعاد 100 و 40 میکرومتر عبور داده شدند. سلول‌های استرومایی حاصل در محیط DMEM/F-12 تا پاساژ 4 کشت شد و سپس به‌مدت 7 روز در دو بخش مطالعه شد: گروه کنترل (بدون هورمون) و گروه‌های آزمون با غلظت‌های متفاوت استروژن (E2)؛ 3/0، 7/0 و 1 نانومول بر لیتر و غلظت ثابت
Adenosine is a multi-functional physiological molecule found abundantly in the body. It is one of the important components of ATP cellular energy metabolism. Adenosine has diverse actions as a ligand on many different types of cells and tissues acting via specific receptors. Currently, four subtypes of adenosine receptors are described, namely, the A1, A2A, A2B and A3 receptors. Neuroblastoma, mostly found in young children, is a malignant tumor derived from peripheral neurons in the body. Several different types of neuroblastoma cell lines of human origin have been established and contributed to the studies of neuroblastoma itself, neuronal differentiation, neurotransmitters, alcoholism, Alzheimers disease and other neuronal diseases and disorders. In 1987, it was shown by Abbracchio et al. that a human neuroblastoma cell line, IMR32, could be induced to differentiate into cells that have a more neuronal morphology, with long neurites, by an adenosine receptor agonist ...
The observation of three distinct patterns (nuclear, cytoplasmic, and both) of localization of FKHR1 suggested the possibility that its intracellular localization might be regulated by extracellular growth signals or cell cycle progression. This hypothesis was tested by determining the localization of FKHR1-HA in serum-starved cells. CV1 cells transiently transfected with FKHR1-HA subsequently were maintained in serum-free medium for 24 hr before fixation. Under conditions of serum starvation FKHR1-HA was restricted to the nucleus in greater than 90% of the cells (Fig. 1 B and I). Moreover, treatment of serum-starved cells expressing FKHR1-HA with either 50 nM insulin-like growth factor I (Fig. 1O) or 10% serum (data not shown) for periods of time as short as 15-30 min was sufficient to cause export of FKHR1-HA from the nucleus in 70% of cells.. The growth factor stimulation of FKHR1-HA nuclear export could reflect either a passive process, such as inhibition of DNA binding leading to a ...
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Fetal bovine serum (FBS) from RMBIO promotes steady, healthy cell growth and is sourced from Australia and New Zealand as well as the US.
Gentaur molecular products has all kinds of products like :search , Equitech \ Sterile filtered fetal bovine serum, dialyzed, triple 0.1 micron filtered, 500ml \ SFBUDY-0500 for more molecular products just contact us
Gentaur molecular products has all kinds of products like :search , Equitech \ Sterile filtered fetal bovine serum, 500ml \ SFBU-0500 for more molecular products just contact us
[118 Pages Report] Check for Discount on 2017-2022 China Fetal Bovine Serum Market Report (Status and Outlook) report by LP Information INC. ...
I have experienced neither depression or long-term mental health problems. The debilitation it manifests is shocking. Over the last few days with great gentleness and dogged determination we have recreated a peaceful home for my eldest sister who does. So why write about this on my blog ? For many years I have found gardening …
Purpose: To compare the cellular uptake efficiency and cytotoxicity of aminosilane (SiO2-NH2)-coated superparamagnetic iron oxide ([email protected]) nanoparticles with three other types of SPIO nanoparticles coated with SiO2 ([email protected]), dextran ([email protected]), or bare SPIO in mammalian cell lines. Materials and methods: Four types of monodispersed SPIO nanoparticles with a SPIO core size of 7 nm and an overall size in a range of 7-15 nm were synthesized. The mammalian cell lines of MCF-7, MDA-MB-231, HT-29, RAW264.7, L929, HepG2, PC-3, U-87 MG, and mouse mesenchymal stem cells (MSCs) were incubated with four types of SPIO nanoparticles for 24 hours in the serum-free culture medium Dulbeccos modified Eagles medium (DMEM) with 4.5 μg/mL iron concentration. The cellular uptake efficiencies of SPIO nanoparticles were compared by Prussian blue staining and intracellular iron quantification. In vitro magnetic resonance imaging of MSC pellets after SPIO labeling was performed at 3 T. The effect of each SPIO
Constitutive centripetal transport of the actin-based cytoskeleton has been detected in cells spreading on a substrate, locomoting fibroblasts and keratocytes, and non-locomoting serum-deprived fibroblasts. These results suggest a gradient of actin assembly, highest in the cortex at the cytoplasm-membrane interface and lowest in the non-cortical perinuclear cytoplasm. We predicted that such a gradient would be maintained in part by phosphoinositide-regulated actin binding proteins because the intracellular free Ca2+ and pH are low and spatially constant in serum-deprived cells. The cytoplasm-membrane interface presents one surface where the assembly of actin is differentially regulated relative to the non-cortical cytoplasm. Several models, based on in vitro biochemistry, propose that phosphoinositide-regulated actin binding proteins are involved in local actin assembly. To test these models in living cells using imaging techniques, we prepared a new fluorescent analog of actin that bound ...
This page segues to comprehensive insights on how riboflavin and other important cell culture components affect the performance of serum-free, protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of riboflavin.
One object of the present invention is based upon the development and use of a serum-free defined cell culture medium comprising a supplement mixture, a component mixture, a vitamin mixture, an inorga
EX-CELL MDCK is a serum-free, animal-protein free medium designed and optimized to support high-density culture of Madin Darby Canine Kidney (MDCK) cells.
هدف: سلول‌های بنیادی پرتوان مشتق شده از بافت بیضه، یک منبع بسیار غنی و جدیدی برای سلول درمانی در پزشکی بازساختی هستند. مطالعات اخیر نشانگر آن است که سلول‌های بنیادی اسپرماتوگونی در شرایط آزمایشگاهی می‌توانند به سلول‌های بنیادی شبه جنینی تغییر یابند.استفاده از امواج فراصوت با شدت پایین، آثار مثبتی روی رشد و تمایز سلول‌ها دارد. هدف از این مطالعه تأثیر امواج فراصوت روی کلونی‌زایی سلول‌های بنیادی شبه جنینی است. مواد و روش‌ها: ابتدا، سلول‌های بنیادی اسپرماتوگونی از بیضه نوزاد موش جدا شدند. بعد از کشت دادن این سلول‌ها در محیط DMEM/F12، حاوی 15 درصد سرم جنین
The culture medium used to grow this cell line is RPMI supplemented with serum. Some characteristics of Raji cell include a ... Raji cells grow as single, non-motile, free-floating (non-adhesion) individuals or doublets to glass. Some cells look elongated ... The limit of sensitivity of this test was 6 mug AHG/ml serum. The ability of Raji cells to detect AHG in serum depended on the ... Moreover, sera from 10 SLE patients that had negative Raji assays contained no IgG that bound to pronase-digested Raji cells. ...
... may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of ... may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media. ... laidlawii has been identified as a common contaminant of growth media for cell culture. A. laidlawii was first isolated from ... Windsor HM; Windsor GD; Noordergraaf JH (March 2010). "The growth and long term survival of Acholeplasma laidlawii in media ...
In culture, cells are surrounded by contaminants. Bovine serum from cell culture media and cellular debris can contaminate the ... To remove these contaminants, cells can be washed with PBS or serum-free medium (SFM) before incubating in SFM and collecting ... Some proteins are secreted in low abundance and then diluted further in the cell culture medium or body fluid, making these ... Supernatant from cells grown in normal medium and cells grown in medium with stable-isotope labeled amino acids is mixed in a 1 ...
It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems. FGF2, in ... Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the ... Soulet F, Al Saati T, Roga S, Amalric F, Bouche G (Nov 2001). "Fibroblast growth factor-2 interacts with free ribosomal protein ... "A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells ...
"Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium". In vitro ... cellular & developmental biology : journal of the Tissue Culture Association. 24 (12): 1223-8. doi:10.1007/bf02624194. PMID ...
Towell's II culture chamber). Since serum was found to be toxic, serum-free media were used, and the special apparatus ... Media solidified with agar are also used for organ culture and these media consist of 7 parts 1% agar in BSS, 3 parts chick ... The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ ... Defined media with or without serum are also used with agar. The medium with agar provides the mechanical support for organ ...
An extract of the material which is being tested is prepared using physiological saline or serum free media (the latter is ... A near confluent layer of fibroblasts are prepared in a culture plate Old cell culture media is removed The cells are covered ... A near confluent layer of fibroblasts are prepared in a culture plate Old cell culture media ([agar] generally) is removed ... which are incubated for 24 hours at 37 degrees Celsius The material is removed The culture media is removed The remaining cells ...
mTeSR1 is a highly specialized, serum-free and complete medium designed for the feeder-free culture of human embryonic stem ... Stem Cell Culture Medium Low Protein Medium for Feeder-Free Culture of Human ES Cells and iPS Cells - TeSR™-E8™ Official ... NeuroCult serum-free media and reagents are available for the culture and characterization of primary neurons, neural stem ... It is developed and manufactured by Primorigen Biosciences StemSpan serum-free media are designed for the culture and expansion ...
GIBCO Perspectives in Cell Culture Invitrogen Guide to Serum-Free Culture TNC Bio list of Chemically Defined products ... Reduced-serum media (commonly 1-5% FBS) Serum-free media (synonymous with Defined media) Protein-free media (no protein but ... There is a clear distinction between serum-free media and chemically defined media. Serum-free media may contain undefined ... using the above definitions this type of media is referred to as serum-free media. Peptide-free, protein-free, chemically ...
... as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this ... The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid ... HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 ... Breitman, T, S. Collins, B. Keene, (1980). "Replacement of serum by insulin and transferrin supports growth and differentiation ...
The S2 cells have been shown to grow up to 5.1×107 cells/ml in serum free medium and above 107 cells/ml in basal media such as ... Several media have been developed for culturing insect cell lines with many of them suitable for culturing S2 cells. ... S2 cells were derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos by Dr. Imogene ... and they can be grown in the absence of serum. ...
RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate ... commonly referred to as RPMI medium or RPMI 1640, is a form of medium used in cell culture and tissue culture used for growing ... www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Mammalian-Cell-Culture/Classical_Media/RPMI. ... Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, ...
... culture media MeSH D27.720.470.305.250 --- culture media, conditioned MeSH D27.720.470.305.255 --- culture media, serum-free ... free radical scavengers MeSH D27.505.519.249 --- chelating agents MeSH D27.505.519.249.410 --- iron chelating agents MeSH ...
... culture media, conditioned MeSH E07.206.255 --- culture media, serum-free MeSH E07.222.210 --- dental articulators MeSH E07.222 ...
... and the 4 seeding medium is then removed and replaced with the experimental culture medium (phenol red free DMEM with charcoal ... with fetal bovine serum (FBS) and phenol red as buffer tracer (culture medium), at 37 °C, in an atmosphere of 5% CO₂ and 95% ... and treated only with hormone-free medium as a negative control. The bioassay ends on day 6 (late exponential phase) by ... To accomplish the E-SCREEN assay the cells are trypsinized and plated in well culture plates. Cells are allowed to attach for ...
FBS-free cell culture media, e.g. with Platelet Lysate or chemically defined/ animal component free, are used for cell therapy ... Laboratory use of serum Laner‑Plamberger et al. J Transl Med (2015) 13:354 Iudicone P (Jan 2014). "Pathogen-free, plasma-poor ... Platelet lysate is commonly used for supplementation of basal media in mesenchymal stem cells culture. Prior the use, the ... The included clotting factors require to add heparin to the cell culture media to prevent coagulation during incubation. ...
... serum free and chemically defined media (CDM) have been developed as a matter of good laboratory practice. Fetal bovine serum ... The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and ... Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to ... Fetal bovine serum, as with the vast majority of animal serum used in cell culture, is produced from blood collected at ...
... been used to isolate and expand CNS stem cells by its ability to aggregate and proliferate hmNPCs under serum-free media ... There are two ways to culture the hmNPCs, the adherent monolayer and the neurosphere culture systems. The neurosphere culture ... NSCs can be cultured in vitro as neurospheres. These neurospheres are composed of neural stem cells and progenitors (NSPCs) ... In previous studies, cultured neurospheres have been transplanted into the brains of immunodeficient neonatal mice and have ...
The extracted inner cell mass was cultured on fibroblasts treated with mitomycin-c in a medium containing serum and conditioned ... and serum-free conditions. After more than 6 months of undifferentiated proliferation, these cells demonstrated the potential ... from the donor mother at approximately 76 hours after copulation and cultured them overnight in a medium containing serum. The ... Martin G (1981). "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by ...
Primary isolates were cultured in ATCC medium 98 agar containing 105 U penicillin G 1−1, 10 5 U polymyin B 1 −1, 65 mg ... Isolates were grown in broth medium with large (20%) and minute (0.2%) amounts of fetal bovine serum as a sterol source. Growth ... The surviving alligator tested free of M. alligatoris after 14 weeks, further supporting the researchers suspicions of the new ... Cultures were diluted in broth medium then passed through membrane filters of various pore diameters, yielding similar results ...
... for expression in serum-free suspension. Since QMCF plasmids contain antibiotic resistance gene and are able to stably ... Finally, the process is switched to production phase by changing media temperature to 30 ̊C. construction of protein ... replicate and remain inside dividing cells, a selection and growth of the cell culture takes place. This allows to upscale the ...
Van der Valk, J (2010). "Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in ... Missing or empty ,title= (help) https://biotechin.asia/2015/12/02/gelzen-inc-making-sustainable-animal-free-gelatin/. Missing ... efforts to remove serum from the growth media are key to the advancement of cellular agriculture as fetal bovine serum has been ... Today the status quo for growing animal tissue in culture involves the use of fetal bovine serum (FBS). FBS is a blood product ...
Egg allergy "Fordras S.A. Nutraceutical Ingredients, Functional Food, Pharmaceutical API And Culture Media : Ovotransferrin". ... Transferrins, are iron binding proteins and acute phase reactants of animal serum. It has a binding log of 15 at a pH of 7 or ... Its structure also consists of fifteen disulfide crosslinks and no free sulfhydryl groups. Disulfide groups stabilize the ... At the C- lobe, human serum has two N-glycans while the hen ovotransferrin has a single N-glycan. Consequently, structurally ...
This process of cell culture or tissue culture requires a method to dissociate the cells from the container and each other. ... Trypsin is inhibited by serum and the divalent cations calcium and magnesium, so serum is usually added to the container once ... In such flasks, cells are provided with growth medium comprising the essential nutrients required for proliferation, and the ... Most commercially available trypsin is of porcine origin, while alternate reagents formulated from animal origin-free ...
It is similarly cytotoxic to malignant gliomas grown in cell culture. Normal (non-tumorous) astrocytes grown in culture are far ... The concentrations of fatty acids in blood serum or plasma can be measured using α-parinaric acid, which will compete for ... as these proteins help protect beer from foam-reducing medium- and long-chain fatty acids. α-Parinaric acid is cytotoxic to ... the process where free radicals react with electrons from cell membrane lipids, resulting in cell damage. ...
"Reaction of nitric oxide with the free sulfhydryl group of human serum albumin yields a sulfenic acid and nitrous oxide". ... "Homocysteine remethylation during nitrous oxide exposure of cells cultured in media containing various concentrations of ... increased oxidative stress via free radical accumulation and decreased NO bioavailability. Free radical accumulation occurs due ... Free text. Li, Chun-Qi; Pang, Bo; Kiziltepe, Tanyel; Trudel, Laura J.; Engelward, Bevin P.; Dedon, Peter C.; Wogan, Gerald N. ( ...
In addition to their antigonadotropic effects, estrogens are also functional antiandrogens by decreasing free concentrations of ... cells in culture". Journal of Steroid Biochemistry. 31 (5): 845-52. doi:10.1016/0022-4731(88)90295-6. PMID 2462135.. ... "Effects of norgestrel and ethinyloestradiol ingestion on serum levels of sex hormones and gonadotrophins in men". Clinical ... Springer Science & Business Media. pp. 71-72, 75, 93. ISBN 978-1-60327-829-4.. ...
... carbon dioxide and a growth medium. Serum-free, nutritious medium provides all the nutrients for the cells to grow. Thus cells ... cartilage explant culture, or blastocyst implant culture. Historically, explant culture has been used in several areas of ... The samples are often minced, and the pieces are placed in a cell culture dish containing growth media. Over time, progenitor ... In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed ...
... s serum-free product portfolio with the addition of two new cell culture media. ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media Corning Announces Expanded Portfolio of Serum-free Cell ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media. Corning Announces Expanded Portfolio of Serum-free. ... MSC Medium capitalize on the growing global demand for specifically defined, serum-free media. The media can enable lab ...
... more and more scientists are considering serum-free cell culturing. In the webinar titled "Cell culture media: Why go serum- ... Free Lonza webinar to discuss the benefits of working with serum-free cell culture media and provide guidance on how to get the ... Lonza to Host New Webinar - "Cell Culture Media: Why Go Serum-Free?" October 01,2019 *News Release PDF ... How to switch from serum to serum-free culturing with little effort ...
Animal-free research. FCS-free - Culture media without fetal calf serum Animal experiments are increasingly being replaced by ... Furthermore, the produced hPL could meet a large percentage of the global demand for animal serum-free culture media. Moreover ... However, there are FCS-free culture media, for example media containing human platelet lysate (hPL). It is produced from human ... The switch to FCS-free culture media is urgent and imperative in order to no longer cause the suffering of millions of calf ...
Keratinocyte cultures with serum or serum free media - (May/17/2005 ). can i know suggestions, ideas and opinions about the ... keratinocyte culture??. should it be cultivated under serum contained media or serum free media??. around what are the seeding ... should it be cultivated under serum contained media or serum free media??. around what are the seeding densities do vary with ... the serum free media??. ur suggestions and ideas are very much appreciated! pls! because im confusing using the serum free ...
... or co-cultured in serum or serum-free medium were compared and analyzed via the platform. It was demonstrated that serum ... However, both cell types were successfully co-cultured in 2D using serum-free medium if the initial cell seeding density was ... When mono-cultured on TCP, both HDFs and HaCat cells were less proliferative in medium without serum than with serum. ... were then conducted successfully in serum-free medium. This study demonstrated that the generic research platform had great ...
Tissue Culture > Media, Serum Free products in the SelectScience products and suppliers directory ... MSC NutriStem® XF Medium. Biological Industries. Defined, xeno-free, serum-free culture medium, Designed to support the ... EndoGo™ XF Medium. Biological Industries. Defined, xeno-free, serum-free medium for long-term expansion of large and small ... NutriStem® hPSC XF Medium. Biological Industries. Defined, xeno-free, serum-free medium, Designed for optimal growth and ...
The recipe for the formulation is given, which provides a medium suitable for the proliferation and differentiation of CD34 + ... A serum-free medium which supports the proliferation and differentiation of CD34 + cells purified from normal bone marrow, ... Serum Free 2 951 Medium 1 Serum Free 2 60 Medium 2 IMDM + 20% 2 78 FBSExpt. Serum Free 2 1402 Medium 1 Serum Free 2 36 Medium 2 ... 1 Serum Free 2 132 Medium 1 Serum Free 2 80 Medium 2 IMDM + 20% FBS 2 132Expt. 2 Serum Free 2 90 Medium 1 StemPro 34 2 62 IMDM ...
CellGenix GMP SCGM medium can be used for serum-free cultivation of HSCs as well as expansion and differentiation of NK & CIK ... Stem Cell Growth Medium. CellGenix GMP SCGM is an optimized, xeno-free medium used for the serum-free expansion of low numbers ... Cytokines & Growth Factors Serum-free Media Bioprocessing Containers Supplements & Cells CellGenix® GMP SCGM. ... Efficient serum-free retroviral gene transfer into primitive human hematopoietic progenitor cells by a defined, high-titer, ...
... Featured Product Nov 21, 2016 ... Expanding the culture of excellence. NutriStem® hPSC XF Medium is a defined, xeno-free, serum-free medium designed to support ... hPSC XF Medium and the leading competing medium for feeder-free culture. Medium was changed and proliferation was assessed ... The defined, xeno-free formulation of NutriStem® hPSC XF Medium provides consistent media performance and predictable cellular ...
Culturing HSPCs in Serum-Free Media for Cell Therapy Research Whether a protocol uses cell culture methods to expand edited ... serum-free medium undergoes rigorous QC testing to ensure consistent conditions for the culture of HSPCs. StemSpan™ media do ... Media Matters: Serum-Free Hematopoietic Cell Culture for Gene Editing and Gene Therapy Research. STEMCELL™ Technologies ... As compared to other serum-free media, StemSpan™ SFEM II medium supports the superior expansion of CD34+ cells when combined ...
... serum - free culture medium / conditioned medium / tumor antigens / growth factors / glutamine ) by Edward M. Alderman et al. ... free medium ( serum - free culture medium / conditioned medium / tumor antigens / growth factors / glutamine ). *. Edward M. ... free medium ( serum - free culture medium / conditioned medium / tumor antigens / growth factors / glutamine )}, author={Edward ...
Global Serum-Free Freezing Culture Media Industry 2020 Global Serum-Free Freezing Culture Media Market 2020 Global Serum-Free ... Global Serum-Free Freezing Culture Media Market Price Global Serum-Free Freezing Culture Media Market Share Global Serum-Free ... Culture Media Market Analysis Global Serum-Free Freezing Culture Media Market Growth Global Serum-Free Freezing Culture Media ... Freezing Culture Media Market Size Global Serum-Free Freezing Culture Media Market Trend ...
Mammary organoids from the glands of female F344 rats were cultured in a serum-free medium. Monolayer culture colonies ... However, colonies cultured in BSFM supplemented with prolactin, E2, and progesterone (complete hormone serum-free medium, CHSFM ... The scrape loading/dye transfer technique showed that most of colonies that developed in a serum-free medium containing EGF, ... human transferrin, insulin, and hydrocortisone (basal serum-free medium, BSFM) failed to show cell-cell communication. ...
Sf9 Serum-free/Protein-free Medium, 1X, With L-glutamine Formulated to support propagation of Sf9 insect cells in culture. ... Gibco™ Essential 8™ Flex Medium Kit Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of ... HyClone™ SFX Insect™ Cell Culture Media is a protein-free media that support cell culture growth. ... SFM4Transfx-293 Media, a serum-free, animal-derived component free media. ...
... protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal ... This page segues to comprehensive insights on how riboflavin and other important cell culture components affect the performance ... Sigmas Cell Culture Media Expert provides in depth discussion of this and other serum-free and protein-free media supplements ... used as a starting formulation for development of proprietary serum-free, or protein-free cell culture media for Chinese ...
... protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page ... How the trace metal selenium and other cell culture components affect the performance of serum-free, ... Serum-Free/Protein Free Hybridoma Medium; Swims S-77 Medium; Waymouth Medium MB; Williams Medium E and various proprietary ... Our Cell Culture Media Expert provides in depth discussion of this and other serum-free and protein-free media supplements. The ...
found in: HyClone™ HyCell™ TransFx-H Media, Phosphate-Buffered Saline (PBS), HyClone™ Molecular Biology Grade Water, Hepatocyte ... Hepatocyte Culture Media Kit Corning. Corning® Hepatocyte Culture Media Kit, a serum-free, fully-defined medium. (500 ml) ... QBSF®-56 Serum Free Medium Quality Biological. QBSF-56 is a proprietary serum-free formulation engineered to support ... medium in the process of weaning fastidious cells from growth in a serum-supplemented medium to a low protein serum-free medium ...
Serum Free and Xeno Free Tissue Culture Media Neuro-PURE™ is the only commercially available 100% Serum Free and Xeno Free ... Serum Free and Xeno Free Tissue Culture Media Neuro-PURE™ is the only commercially available 100% Serum Free and Xeno Free ... Serum Free and Xeno Free Tissue Culture Media. Neuro-PURE™ is the only commercially available 100% Serum Free and Xeno Free ... Serum Free and Xeno Free Tissue Culture Media. Jeeven BioSciences, Inc. Announces the Release of its Neuro-PURE™ Serum … ...
Serum-free cell culture medium. Introduction. Serum-free mediums allow users to standardize their cell culture conditions. It ... serum-free medium, e.g. 15 ml DMEM (not serum-free) + 5 ml serum-free medium (FreeStyle™ 293 Expression Medium) for a T75 flask ... Testing serum free medium. Serum free medium was obtained from AAV-293 cells are not adapted to serum-free growth conditions so ... We raised the serum-free ratio to 100 % over 7 passages.. 100% serum-free cells grow slower compared to the serum-supplemented ...
Culture Media, Serum Free. Active Comparator: Optisol GS Donor cornea is stored in the Optisol GS media prior to implantation. ... Active Comparator: Life 4°C media for cornea storage Donor cornea is stored in the Life 4°C media prior to implantation. ... Other: Corneal donor storage in Optisol GS media solution Donor tissue is preserved in the Optisol media until ready for ... Life 4°C Versus Optisol in Corneal Storage Media. The safety and scientific validity of this study is the responsibility of the ...
... authenticated cells with their ideal media and transfection reagents to create optimized, easy to use protein expression ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Serum-Free VERO Cell System Host and Packaging Cell Lines ATCC holdings include an array of host and packaging cell lines of ... ATCC Protein and Virus Production Systems match reliable, authenticated cells with their ideal media and reagents to create ...
Serum free cell culture medium. US5118666. Jun 1, 1990. Jun 2, 1992. The General Hospital Corporation. Insulinotropic hormone. ... Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:RINELLA, JOSEPH VINCENT, JR.;REEL/FRAME:011736/0793 ... making possible a multi-use parenteral formulation that is relatively free of protein aggregation. ...
Serum free culture media. *Starting material. *Reproducible end product. 10:55 Morning Coffee. ... 10:20 STANDARDIZATION OF STEM CELL CULTURING. Judit Nagy, Research Scientist, Director Proteomics Facility, Imperial College. * ...
... the first serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (hMSCs) ... First serum-free medium for hMSCs * Superior efficiency of hMSC expansion at high cell densities-less medium, surface area, and ... CELLstart™ - Substrate for serum-free hMSC culture CELLstart™ is designed for use with STEMPRO® MSC SFM as a substrate to ... Culture of hMSC under serum-free conditions using StemPro MSC SFM - paper published in Biochem. Biophys. Res. Commun ...
Retracted] Serumfreemedium‑type mesenchymal stem cell culture supernatant exerts a protective effect on A549 lung epithelial ... Wu, J., Shang, A., Chen, C., Wang, W., Xiong, C., Guo, N.[Retracted] Serumfreemedium‑type mesenchymal stem cell culture ... Wu, J., Shang, A., Chen, C., Wang, W., Xiong, C., Guo, N.[Retracted] Serumfreemedium‑type mesenchymal stem cell culture ... The authors wish to retract their research article entitled Serum-free-medium-type mesenchymal stem cell culture supernatant ...
  • The Corning stemgro MSC Medium was designed for the maximum expansion of human mesenchymal stem cells (MSCs) derived from bone marrow, cord blood, or adipose tissue. (corning.com)
  • A generic research platform with 2-dimensional (2D) cell culture technology, a 3-dimensional (3D) in vitro tissue model, and a scaled-down cell culture and imaging system in between, was utilized to address the problematic issues associated with the use of serum in skin tissue engineering. (mdpi.com)
  • It was demonstrated that serum depletion had significant influence on the attachment of HaCat cells onto tissue culture plastic (TCP), porous substrates and cellulosic scaffolds, which was further enhanced by the pre-seeded HDFs. (mdpi.com)
  • Tissue culture is one of the most widespread laboratory techniques. (selectscience.net)
  • Primary cultures may be derived from blood or tissue explants and thousands of immortalized cell lines are now available from tissue culture collections. (selectscience.net)
  • The media also effectively grows mouse cells and other tissue as well. (jeevanbiosciences.com)
  • Several supports have then been tested including tissue culture polystyrene (as a reference), teflon, polycarbonate or poly(ethylene terephthalate) track-etched membranes. (uclouvain.be)
  • In the present study, porcine embryonic (28-30 days), ventral mesencephalic precursor cells were isolated and propagated as free-floating neural tissue spheres in medium containing epidermal growth factor and fibroblast growth factor 2. (hindawi.com)
  • In order to avoid mechanical and enzymatic dissociation and to preserve an organotypic intercellular microenvironment during propagation of neural precursor cells, culturing of tissue microexplants as neural tissue spheres (NTS) was established in our laboratory [ 19 , 20 ]. (hindawi.com)
  • In contrast to the classical cell-aggregate neurospheres [ 21 , 22 ], cells in NTS cultures are never dissociated, neither at the time of tissue sampling and culture set up nor at cell passaging. (hindawi.com)
  • Based on application, the cell culture market is categorized into biopharmaceutical production, stem cell research, diagnostics, drug discovery & development, tissue engineering & regenerative medicine, and other applications. (marketpublishers.com)
  • For reference see: Morton, H.C., A survey of Commercially Available Tissue Culture Media. (coriell.org)
  • It should be edible, animal-free, and must be capable of expanding and contracting like normal muscle tissue. (sgs.com)
  • These results established that DP-MSCs constitute a heterogeneous mixture of cells in pulp tissue in vivo and in culture, and that their phenotype is modified upon in vitro expansion. (frontiersin.org)
  • NutriStem® hPSC XF Medium offers the ability to culture human pluripotent cells without the need for high levels of bFGF and other stimulatory growth factors or cytokines. (technologynetworks.com)
  • StemSpan™ media do not contain cytokines or growth factors, allowing researchers the flexibility to establish cultures that meet their specific requirements. (regmednet.com)
  • In course of time experiments were performed in which serum enriched with hormones and other growth factors was used to successfully culti- vate those cells which could not survive in serum-supplemented media alone. (springer.com)
  • Growth factors can alleviate that stress and are critical for long-term growth and proliferation of many cell lines in serum-free media formulations. (repligen.com)
  • In vitro culture of stem cells must be a carefully controlled process, and different stem cell types (such as embryonic, mesenchymal, hematopoietic and neural) each require specific media components, such as supplementation, growth factors and other signaling molecules. (biocompare.com)
  • A number of bioactive factors like cytokines and growth factors secreted by MSCs in the conditioned medium are very likely to be the principle molecules which play a vital role in skin regeneration. (hindawi.com)
  • This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications. (wikipedia.org)
  • Serum is stored frozen to preserve the stability of components such as growth factors. (wikipedia.org)
  • To date a standard component of culture media has been the blood serum from unborn calves. (aerzte-gegen-tierversuche.de)
  • This is, however, obtained from unborn calves and is therefore incompatible with the objective of being free from animal products. (sgs.com)
  • Although anoxia or active slaughter could be used to induce unconsciousness or death prior to serum harvesting, exposure of live unborn calves to oxygen can cause them to gain awareness before being killed, resulting in active debate about the ethics of harvesting serum. (wikipedia.org)
  • We routinely freeze cells in 10%DMSO - 90%FCS, and have NOT been successful in freezing hybridoma cells that have been adapted to serum free conditions. (bio.net)
  • In cell biology, cells are commonly grown in medium supplemented with serum. (lonza.com)
  • The cells were used for AAV production, and we produced similar amounts of virus particles compared to cells grown in FCS-supplemented medium. (igem.org)
  • The cells were grown encapsulated in calcium alginate microspheres under serum-free conditions for 10 days. (biomedsearch.com)
  • They proved that cultured cells could be grown into various structed or semi-structed components and, in 1995, the US Food and Drug Administration (FDA) approved the use of in-vitro techniques for the commercial production of meat. (sgs.com)
  • Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their specific cell type. (wikipedia.org)
  • In this report, we discuss our findings about a scalp psoriasis suffering patient with a Psoriasis Scalp Severity Index (PSSI) score of 28, who was treated with Mesenchymal stem cell conditioned media (MSC-CM). Remarkably, complete regression was recorded within a treatment period of one month only (PSSI score of 0). (hindawi.com)
  • The Corning Hepatocyte Maintenance Medium is optimized to cultivate primary human hepatocytes, a well-established in vitro model system for studying preclinical drug-induced liver injury (DILI). (corning.com)
  • Animal experiments are increasingly being replaced by cell cultures and organs-on-chips, utilizing human cells derived as waste materials after surgeries. (aerzte-gegen-tierversuche.de)
  • For their survival and growth, the human cells need to be immersed in a culture medium, i.e. a solution containing different nutrients. (aerzte-gegen-tierversuche.de)
  • In addition, there is a wide range of other FCS-free culture media that are derived synthetically or from human blood. (aerzte-gegen-tierversuche.de)
  • The present invention relates to a serum-free medium which can support the growth and proliferation of normal human hematopoietic CD34 + cells purified from sources such as normal human bone marrow, the peripheral blood of patients treated with cytokines (termed mobilized CD34 + cells) or umbilical cord blood. (google.com)
  • In order to modify or expand primary human hematopoietic cells, they must first be isolated from the body and then cultured under conditions that allow for their modification. (regmednet.com)
  • This group cultured human CD34+ cells in StemSpan™ medium and cytokines as a pre-stimulation step ahead of electroporation, before xenotransplantation into mice or additional in vitro culture. (regmednet.com)
  • Lymphocyte Separation Medium (LSM™) is a legendary tool to separate mononuclear cells from human peripheral blood as well as bone marrow and umbilical cord blood. (mpbio.com)
  • In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. (springer.com)
  • Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today. (springer.com)
  • Human iPSCs cultured using Primate ES Cell Medium with 5 ng/mL bFGF on MEF cells. (reprocell.com)
  • This stem cell media enabled development of the world's first human induced pluripotent stem cells in 2007 by Prof Shinya Yamanaka and his colleagues. (reprocell.com)
  • This stem cell media has been specifically developed to simplify the process of ES and iPS cell culture for human and other primate cells. (reprocell.com)
  • Serum withdrawal up-regulates human SIRT1 gene expression in a p53-dependent manner. (nih.gov)
  • We found that serum withdrawal also up-regulates human SIRT1 gene expression in a p53-dependent manner and that the p53-binding element in SIRT1 is required for the up-regulation. (nih.gov)
  • A putative p53 response element present in the SIRT1 promoter contributes to the induction of human SIRT1 expression by serum withdrawal. (nih.gov)
  • Same amount of luciferase constructs containing mouse Sirt1 or human SIRT1 were transfected into HEK293 and U2OS cells and cells were maintained in normal nutrients (C) or under serum starvation (S). Cells were harvested 16 hrs after treatments and luciferase activities were determined. (nih.gov)
  • Culture media for human and animal cells can contain a variety of ingredients. (miltenyibiotec.com)
  • Fetal bovine serum, as with the vast majority of animal serum used in cell culture, is produced from blood collected at commercial slaughterhouses from dairy cattle that also supply meat intended for human consumption. (wikipedia.org)
  • Here we show using cultured DRG neurons, that of the total potassium current, I K , the K Na current is predominantly inhibited by PKA. (jneurosci.org)
  • Here we demonstrate that PKA activation in cultured DRG neurons produces hyperexcitability, a reduction in the K Na component of I K and Slack channel internalization. (jneurosci.org)
  • This study analyses voltage-sensitive ionic currents of cultured antennal lobe projection neurons and mushroom body Kenyon cells in the brain of the honeybee Apis mellifera . (biologists.org)
  • One of the major issues in cell culture is mycoplasma infection, altering a variety of cellular characteristics and functionalities and leading to experimental artifacts and cell loss. (mpbio.com)
  • The unique mycoplasma detection kit and Mycoplasma Removal Agent (MRA) can completely manage the mycoplasma contamination in your cell culture. (mpbio.com)
  • Once Mycoplasma has been detected, the infected cell culture should be treated with Mycoplasma Removal Agent (MRA), the most reliable solution for mycoplasma removal and prevention, as recognized in more than 550 scientific publications. (mpbio.com)
  • Constructs with native (P-158) or mutant p53 response element (P-158mut) or without p53 response element (P-111) were transfected into U2OS and HepG2 cells, and the relative luciferase activity under either normal nutrients (C) or serum-starved condition (S) was determined. (nih.gov)
  • For convenience and reproducibility, a number of commercial stem cell media are commercially available in ready-to-use formulations. (biocompare.com)
  • Here we found that serum deprivation increased caspase-dependent apoptosis through miRNA-101-3p downregulation, without altering expression of its host gene RNA 3′-terminal phosphate cyclase-like 1, which was highly correlated with suppressed expression levels of Dicer and Argonaute 2 (Ago2), indicating that miR-101-3p is post-transcriptionally elevated in serum-deprived conditions. (nature.com)
  • Whether you require an antibiotic active against gram-positive bacteria, gram-negative bacteria, yeast, or fungi, MP Biomedicals provides a wide range of high-quality antibiotics to treat cell culture contamination. (mpbio.com)
  • These easy-to-use, effective antibiotics can efficiently keep cell cultures contamination-free from a wide range of common microbiological contaminants. (mpbio.com)