Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.
The beta subunit of human CHORIONIC GONADOTROPIN. Its structure is similar to the beta subunit of LUTEINIZING HORMONE, except for the additional 30 amino acids at the carboxy end with the associated carbohydrate residues. HCG-beta is used as a diagnostic marker for early detection of pregnancy, spontaneous abortion (ABORTION, SPONTANEOUS); ECTOPIC PREGNANCY; HYDATIDIFORM MOLE; CHORIOCARCINOMA; or DOWN SYNDROME.
The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.
A product of the PLACENTA, and DECIDUA, secreted into the maternal circulation during PREGNANCY. It has been identified as an IGF binding protein (IGFBP)-4 protease that proteolyzes IGFBP-4 and thus increases IGF bioavailability. It is found also in human FIBROBLASTS, ovarian FOLLICULAR FLUID, and GRANULOSA CELLS. The enzyme is a heterotetramer of about 500-kDa.
Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.
CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.
A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.
FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form.
A glycoprotein hormone secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Thyrotropin stimulates THYROID GLAND by increasing the iodide transport, synthesis and release of thyroid hormones (THYROXINE and TRIIODOTHYRONINE). Thyrotropin consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the pituitary glycoprotein hormones (TSH; LUTEINIZING HORMONE and FSH), but the beta subunit is unique and confers its biological specificity.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Blood proteins that bind to THYROID HORMONES such as THYROXINE and transport them throughout the circulatory system.
A prenatal ultrasonography measurement of the soft tissue behind the fetal neck. Either the translucent area below the skin in the back of the fetal neck (nuchal translucency) or the distance between occipital bone to the outer skin line (nuchal fold) is measured.
Blood tests used to evaluate the functioning of the thyroid gland.
A syndrome that results from abnormally low secretion of THYROID HORMONES from the THYROID GLAND, leading to a decrease in BASAL METABOLIC RATE. In its most severe form, there is accumulation of MUCOPOLYSACCHARIDES in the SKIN and EDEMA, known as MYXEDEMA.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Elements of limited time intervals, contributing to particular results or situations.
The beginning third of a human PREGNANCY, from the first day of the last normal menstrual period (MENSTRUATION) through the completion of 14 weeks (98 days) of gestation.
A group of related diseases characterized by an unbalanced or disproportionate proliferation of immunoglobulin-producing cells, usually from a single clone. These cells frequently secrete a structurally homogeneous immunoglobulin (M-component) and/or an abnormal immunoglobulin.
One of the types of light chain subunits of the immunoglobulins with a molecular weight of approximately 22 kDa.
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
A chromosome disorder associated either with an extra chromosome 21 or an effective trisomy for chromosome 21. Clinical manifestations include hypotonia, short stature, brachycephaly, upslanting palpebral fissures, epicanthus, Brushfield spots on the iris, protruding tongue, small ears, short, broad hands, fifth finger clinodactyly, Simian crease, and moderate to severe INTELLECTUAL DISABILITY. Cardiac and gastrointestinal malformations, a marked increase in the incidence of LEUKEMIA, and the early onset of ALZHEIMER DISEASE are also associated with this condition. Pathologic features include the development of NEUROFIBRILLARY TANGLES in neurons and the deposition of AMYLOID BETA-PROTEIN, similar to the pathology of ALZHEIMER DISEASE. (Menkes, Textbook of Child Neurology, 5th ed, p213)
Natural hormones secreted by the THYROID GLAND, such as THYROXINE, and their synthetic analogs.
A highly vascularized endocrine gland consisting of two lobes joined by a thin band of tissue with one lobe on each side of the TRACHEA. It secretes THYROID HORMONES from the follicular cells and CALCITONIN from the parafollicular cells thereby regulating METABOLISM and CALCIUM level in blood, respectively.
A condition in which total serum protein level is below the normal range. Hypoproteinemia can be caused by protein malabsorption in the gastrointestinal tract, EDEMA, or PROTEINURIA.
Electrophoresis applied to BLOOD PROTEINS.
Abnormally elevated THYROXINE level in the BLOOD.
A plant genus of the family RANUNCULACEAE that contains alpha-hederin, a triterpene saponin in the seeds, and is the source of black seed oil.
A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
One of the types of light chains of the immunoglobulins with a molecular weight of approximately 22 kDa.
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
Conditions of abnormal THYROID HORMONES release in patients with apparently normal THYROID GLAND during severe systemic illness, physical TRAUMA, and psychiatric disturbances. It can be caused by the loss of endogenous hypothalamic input or by exogenous drug effects. The most common abnormality results in low T3 THYROID HORMONE with progressive decrease in THYROXINE; (T4) and TSH. Elevated T4 with normal T3 may be seen in diseases in which THYROXINE-BINDING GLOBULIN synthesis and release are increased.
A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.
Hypersecretion of THYROID HORMONES from the THYROID GLAND. Elevated levels of thyroid hormones increase BASAL METABOLIC RATE.
Inflammation of the MIDDLE EAR including the AUDITORY OSSICLES and the EUSTACHIAN TUBE.
Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.
A hypermetabolic syndrome caused by excess THYROID HORMONES which may come from endogenous or exogenous sources. The endogenous source of hormone may be thyroid HYPERPLASIA; THYROID NEOPLASMS; or hormone-producing extrathyroidal tissue. Thyrotoxicosis is characterized by NERVOUSNESS; TACHYCARDIA; FATIGUE; WEIGHT LOSS; heat intolerance; and excessive SWEATING.
Methods for maintaining or growing CELLS in vitro.
Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A condition characterized by a dry, waxy type of swelling (EDEMA) with abnormal deposits of MUCOPOLYSACCHARIDES in the SKIN and other tissues. It is caused by a deficiency of THYROID HORMONES. The skin becomes puffy around the eyes and on the cheeks. The face is dull and expressionless with thickened nose and lips.
Pathological processes involving the THYROID GLAND.
A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC 1.11.1.8.
Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.
Determination of the nature of a pathological condition or disease in the postimplantation EMBRYO; FETUS; or pregnant female before birth.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
Agents that are used to treat hyperthyroidism by reducing the excessive production of thyroid hormones.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
The relationship between the dose of an administered drug and the response of the organism to the drug.
A process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A group of sporadic, familial and/or inherited, degenerative, and infectious disease processes, linked by the common theme of abnormal protein folding and deposition of AMYLOID. As the amyloid deposits enlarge they displace normal tissue structures, causing disruption of function. Various signs and symptoms depend on the location and size of the deposits.
Spontaneously remitting inflammatory condition of the THYROID GLAND, characterized by FEVER; MUSCLE WEAKNESS; SORE THROAT; severe thyroid PAIN; and an enlarged damaged gland containing GIANT CELLS. The disease frequently follows a viral infection.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
Glucose in blood.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A thioureylene antithyroid agent that inhibits the formation of thyroid hormones by interfering with the incorporation of iodine into tyrosyl residues of thyroglobulin. This is done by interfering with the oxidation of iodide ion and iodotyrosyl groups through inhibition of the peroxidase enzyme.
A tripeptide that stimulates the release of THYROTROPIN and PROLACTIN. It is synthesized by the neurons in the PARAVENTRICULAR NUCLEUS of the HYPOTHALAMUS. After being released into the pituitary portal circulation, TRH (was called TRF) stimulates the release of TSH and PRL from the ANTERIOR PITUITARY GLAND.
The principal sterol of all higher animals, distributed in body tissues, especially the brain and spinal cord, and in animal fats and oils.
Observation of a population for a sufficient number of persons over a sufficient number of years to generate incidence or mortality rates subsequent to the selection of the study group.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The rate dynamics in chemical or physical systems.
A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed)
A common form of hyperthyroidism with a diffuse hyperplastic GOITER. It is an autoimmune disorder that produces antibodies against the THYROID STIMULATING HORMONE RECEPTOR. These autoantibodies activate the TSH receptor, thereby stimulating the THYROID GLAND and hypersecretion of THYROID HORMONES. These autoantibodies can also affect the eyes (GRAVES OPHTHALMOPATHY) and the skin (Graves dermopathy).
Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Pathophysiological conditions of the FETUS in the UTERUS. Some fetal diseases may be treated with FETAL THERAPIES.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Established cell cultures that have the potential to propagate indefinitely.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
The age of the mother in PREGNANCY.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.
Techniques used in studying bacteria.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Inflammatory disease of the THYROID GLAND due to autoimmune responses leading to lymphocytic infiltration of the gland. It is characterized by the presence of circulating thyroid antigen-specific T-CELLS and thyroid AUTOANTIBODIES. The clinical signs can range from HYPOTHYROIDISM to THYROTOXICOSIS depending on the type of autoimmune thyroiditis.
Backflow of blood from the RIGHT VENTRICLE into the RIGHT ATRIUM due to imperfect closure of the TRICUSPID VALVE.
Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Proteins prepared by recombinant DNA technology.
The part of a human or animal body connecting the HEAD to the rest of the body.
The main glucocorticoid secreted by the ADRENAL CORTEX. Its synthetic counterpart is used, either as an injection or topically, in the treatment of inflammation, allergy, collagen diseases, asthma, adrenocortical deficiency, shock, and some neoplastic conditions.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The sum of the weight of all the atoms in a molecule.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.
A constituent of STRIATED MUSCLE and LIVER. It is an amino acid derivative and an essential cofactor for fatty acid metabolism.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A 191-amino acid polypeptide hormone secreted by the human adenohypophysis (PITUITARY GLAND, ANTERIOR), also known as GH or somatotropin. Synthetic growth hormone, termed somatropin, has replaced the natural form in therapeutic usage such as treatment of dwarfism in children with growth hormone deficiency.
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Abstaining from all food.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.
The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated as the time from the last day of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization.
In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Proteins found in any species of bacterium.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
Physiological processes and properties of the BLOOD.
Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
A test to determine the ability of an individual to maintain HOMEOSTASIS of BLOOD GLUCOSE. It includes measuring blood glucose levels in a fasting state, and at prescribed intervals before and after oral glucose intake (75 or 100 g) or intravenous infusion (0.5 g/kg).
Diminished effectiveness of INSULIN in lowering blood sugar levels: requiring the use of 200 units or more of insulin per day to prevent HYPERGLYCEMIA or KETOSIS.
All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)
A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
The means of interchanging or transmitting and receiving information. Historically the media were written: books, journals, newspapers, and other publications; in the modern age the media include, in addition, radio, television, computers, and information networks.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Substances that influence the course of a chemical reaction by ready combination with free radicals. Among other effects, this combining activity protects pancreatic islets against damage by cytokines and prevents myocardial and pulmonary perfusion injuries.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Inorganic salts of phosphoric acid.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A MADS domain-containing transcription factor that binds to the SERUM RESPONSE ELEMENT in the promoter-enhancer region of many genes. It is one of the four founder proteins that structurally define the superfamily of MADS DOMAIN PROTEINS.
Transport proteins that carry specific substances in the blood or across cell membranes.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Substances used to allow enhanced visualization of tissues.
A series of steps taken in order to conduct research.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Antibodies produced by a single clone of cells.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Signal molecules that are involved in the control of cell growth and differentiation.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Morphological and physiological development of EMBRYOS or FETUSES.
Substances that reduce the growth or reproduction of BACTERIA.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
A ubiquitous sodium salt that is commonly used to season food.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC 3.4.24.3.
A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Glycoproteins which have a very high polysaccharide content.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.

A telomere-independent senescence mechanism is the sole barrier to Syrian hamster cell immortalization. (1/2283)

Reactivation of telomerase and stabilization of telomeres occur simultaneously during human cell immortalization in vitro and the vast majority of human cancers possess high levels of telomerase activity. Telomerase repression in human somatic cells may therefore have evolved as a powerful resistance mechanism against immortalization, clonal evolution and malignant progression. The comparative ease with which rodent cells immortalize in vitro suggests that they have less stringent controls over replicative senescence than human cells. Here, we report that Syrian hamster dermal fibroblasts possess substantial levels of telomerase activity throughout their culture life-span, even after growth arrest in senescence. In our studies, telomerase was also detected in uncultured newborn hamster skin, in several adult tissues, and in cultured fibroblasts induced to enter the post-mitotic state irreversibly by serum withdrawal. Transfection of near-senescent dermal fibroblasts with a selectable plasmid vector expressing the SV40 T-antigen gene resulted in high-frequency single-step immortalization without the crisis typically observed during the immortalization of human cells. Collectively, these data provide an explanation for the increased susceptibility of rodent cells to immortalization (and malignant transformation) compared with their human equivalents, and provide evidence for a novel, growth factor-sensitive, mammalian senescence mechanism unrelated to telomere maintenance.  (+info)

Growth-inhibitory effect of cyclic GMP- and cyclic AMP-dependent vasodilators on rat vascular smooth muscle cells: effect on cell cycle and cyclin expression. (2/2283)

1. The possibility that the antiproliferative effect of cyclic GMP- and cyclic AMP-dependent vasodilators involves an impaired progression of vascular smooth muscle cells (VSMC) through the cell cycle and expression of cyclins, which in association with the cyclin-dependent kinases control the transition between the distinct phases of the cell cycle, was examined. 2. FCS (10%) stimulated the transition of quiescent VSMC from the G0/G1 to the S phase (maximum within 18-24 h and then to the G2/M phase (maximum within 22-28 h). Sodium nitroprusside and 8-Br-cyclic GMP, as well as forskolin and 8-Br-cyclic AMP markedly reduced the percentage of cells in the S phase after FCS stimulation. 3. FCS stimulated the low basal protein expression of cyclin D1 (maximum within 8-24 h) and E (maximum within 8-38 h) and of cyclin A (maximum within 14-30 h). The stimulatory effect of FCS on cyclin D1 and A expression was inhibited, but that of cyclin E was only minimally affected by the vasodilators. 4. FCS increased the low basal level of cyclin D1 mRNA after a lag phase of 2 h and that of cyclin A after 12 h. The vasodilators significantly reduced the FCS-stimulated expression of cyclin D1 and A mRNA. 5. These findings indicate that cyclic GMP- and cyclic AMP-dependent vasodilators inhibit the proliferation of VSMC by preventing the progression of the cell cycle from the G0/G1 into the S phase, an effect which can be attributed to the impaired expression of cyclin D1 and A.  (+info)

Mycophenolate mofetil inhibits rat and human mesangial cell proliferation by guanosine depletion. (3/2283)

BACKGROUND: Mycophenolate mofetil (MMF) is used for immunosuppression after renal transplantation because it reduces lymphocyte proliferation by inhibiting inosine monophosphate dehydrogenase (IMPDH) in lymphocytes and GTP biosynthesis. In the present study we asked if therapeutic concentrations of MMF might interfere with mesangial cell (MC) proliferation which is involved in inflammatory proliferative glomerular diseases. METHODS: Rat and human MCs were growth-arrested by withdrawal of fetal calf serum (FCS) and stimulated by addition of FCS, platelet-derived growth factor (PDGF) or lysophosphatidic acid (LPA). Different concentrations of MMF (0.019-10 microM) were added concomitantly in the presence or absence of guanosine. MC proliferation was determined by [3H]thymidine incorporation. Cell viability was assessed by trypan blue exclusion. Apoptotic nuclei were stained using the Hoechst dye H33258. Cytosolic free Ca2+ concentrations were determined with the fluorescent calcium chelator fura-2-AM. RESULTS: MMF inhibited mitogen-induced rat MC proliferation with an IC50 of 0.45 +/- 0.13 microM. Human MCs proved to be even more sensitive (IC50 0.19 +/- 0.06 microM). Inhibition of MC proliferation was reversible and not accompanied by cellular necrosis or apoptosis. Addition of guanosine prevented the antiproliferative effect of MMF, indicating that inhibition of IMPDH is responsible for decreased MC proliferation. Early signalling events of GTP-binding-protein-coupled receptors, such as changes in intracellular Ca2+ levels were not affected by MMF. CONCLUSIONS: The results show that MMF has a concentration-dependent antiproliferative effect on cultured MCs in the therapeutic range, which might be a rationale for the use of this drug in the treatment of mesangial proliferative glomerulonephritis.  (+info)

Altered expression of the IGF-1 receptor in a tamoxifen-resistant human breast cancer cell line. (4/2283)

The relationship between oestrogen (E2) and insulin-like growth factor-one (IGF-1) was examined in both tamoxifen-sensitive (MCF 7/5-21) and tamoxifen-resistant (MCF 7/5-23) subclones of the MCF 7 cell line. Both subclones were grown in defined, serum-free (SF) medium over a period of 7 days with the addition of E2 or IGF-1 or a combination of both agents. Growth of both MCF 7/5-21 and 7/5-23 cells was stimulated (245% and 350%, respectively) by E2. However, only the growth of MCF 7/5-23 cells was stimulated (266%) by IGF-1. A combination of E2 and IGF-1 significantly enhanced MCF 7/5-21 and 7/5-23 cell growth (581% and 695%, respectively). E2-induced IGF-1 receptor (IGF-1R) levels (as measured by 125I-IGF-1 binding and Northern analyses) in only MCF 7/5-23 cells. This effect was partially inhibited by tamoxifen. In medium containing serum, the growth of only the MCF 7/5-23 cells was significantly inhibited by the IGF-1R monoclonal antibody, alphaIR-3. The detection of E2-induced expression of IGF-2 using RT-PCR was demonstrated in the MCF 7/5-23 cells. These experiments indicate that E2 may sensitize tamoxifen-resistant MCF 7/5-23 cells to the growth stimulatory actions of IGF-2 via up-regulation of the IGF-1R and describes a cell-survival mechanism that may manifest itself as tamoxifen resistance.  (+info)

Protection against necrosis but not apoptosis by heat-stress proteins in vascular smooth muscle cells: evidence for distinct modes of cell death. (5/2283)

We have reported previously that cultured vascular smooth muscle cells (VSMC) isolated from spontaneously hypertensive rats (SHR) show higher proliferation and cell death than normotensive controls. In addition to protecting cells against death, heat stress proteins (HSPs) appear to play a role in cell proliferation. This investigation examines the involvement of HSP72 and HSP27 in altered SHR VSMC proliferation and death. We have performed detailed discriminatory analysis to characterize which type of VSMC death is induced by heat stress (HS) and serum deprivation. Serum deprivation induced apoptosis (caspase-3 cleavage and DNA laddering) and secondary necrosis, the 2 processes being a continuum of each other. In contrast, acute HS (46 degrees C, 30 minutes), which inhibited BN. lx and SHR VSMC proliferation by 2-fold, increased necrosis (by 5-fold and 2-fold, respectively) but not apoptosis. HSP72 and HSP27 expression evoked in VSMC by mild HS (44 degrees C, 15 minutes) 6 hours before acute HS prevented the inhibition of proliferation and induction of necrosis with no effect on serum deprivation-induced or staurosporine-induced apoptosis. This induced expression of HSP72 and HSP27 did not eliminate the higher basal proliferation, apoptosis, and necrosis of SHR VSMC compared with BN.lx VSMC, suggesting that these HSPs are not involved in altered SHR VSMC proliferation and death. Also, although apoptosis and necrosis may be a continuum, in VSMC the 2 processes may be distinguished by HS, in which only necrosis is prevented by prior HSP accumulation. This observation may be of use in designing strategies for cellular protection.  (+info)

O-glycosylation potential of lepidopteran insect cell lines. (6/2283)

The enzyme activities involved in O-glycosylation have been studied in three insect cell lines, Spodoptera frugiperda (Sf-9), Mamestra brassicae (Mb) and Trichoplusia ni (Tn) cultured in two different serum-free media. The structural features of O-glycoproteins in these insect cells were investigated using a panel of lectins and the glycosyltransferase activities involved in O-glycan biosynthesis of insect cells were measured (i.e., UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP-Gal:core-1 beta1, 3-galactosyltransferase, CMP-NeuAc:Galbeta1-3GalNAc alpha2, 3-sialyltransferase, and UDP-Gal:Galbeta1-3GalNAc alpha1, 4-galactosyltransferase activities). First, we show that O-glycosylation potential depends on cell type. All three lepidopteran cell lines express GalNAcalpha-O-Ser/Thr antigen, which is recognized by soy bean agglutinin and reflects high UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase activity. Capillary electrophoresis and mass spectrometry studies revealed the presence of at least two different UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases in these insect cells. Only some O-linked GalNAc residues are further processed by the addition of beta1,3-linked Gal residues to form T-antigen, as shown by the binding of peanut agglutinin. This reflects relative low levels of UDP-Gal:core-1 beta1,3-galactosyltransferase in insect cells, as compared to those observed in mammalian control cells. In addition, we detected strong binding of Bandeiraea simplicifolia lectin-I isolectin B4 to Mamestra brassicae endogenous glycoproteins, which suggests a high activity of a UDP-Gal:Galbeta1-3GalNAc alpha1, 4-galactosyltransferase. This explains the absence of PNA binding to Mamestra brassicae glycoproteins. Furthermore, our results substantiated that there is no sialyltransferase activity and, therefore, no terminal sialic acid production by these cell lines. Finally, we found that the culture medium influences the O-glycosylation potential of each cell line.  (+info)

Flow cytometric cell cycle analysis of cultured porcine fetal fibroblast cells. (7/2283)

Normal development of nuclear transfer embryos is thought to be dependent on transferral of nuclei in G0 or G1 phases of the cell cycle. Therefore, we investigated the cell cycle characteristics of porcine fetal fibroblast cells cultured under a variety of cell cycle-arresting treatments. This was achieved by using flow cytometry to simultaneously measure cellular DNA and protein content, enabling the calculation of percentages of cells in G0, G1, S, and G2+M phases of the cell cycle. Cultures that were serum starved for 5 days contained higher (p < 0.05) percentages of G0+G1 (87.5 +/- 0. 7) and G0 cells alone (48.3 +/- 9.7) compared with rapidly cycling cultures (G0+G1: 74.1 +/- 3.0; G0: 2.8 +/- 1.2). Growth to confluency increased (p < 0.05) G0+G1 percentages (85.1 +/- 2.8) but did not increase G0 percentages (6.0 +/- 5.3) compared to those in cycling cultures. Separate assessment of small-, medium-, and large-sized cells showed that as the cell size decreased from large to small, percentages of cells in G0+G1 and G0 alone increased (p < 0.05). We found 95.2 +/- 0.3% and 72.2 +/- 12.0% of small serum-starved cells in G0+G1 and G0 alone, respectively. Cultures were also treated with cell cycle inhibitors. Treatment with dimethyl sulfoxide (1%) or colchicine (0.5 microM) increased percentages of cells in G0 (24.8 +/- 20.0) or G2+M (37.4 +/- 4.6), respectively. However, cells were only slightly responsive to mimosine treatment. A more complete understanding of the cell cycle of donor cells should lead to improvements in the efficiency of nuclear transfer procedures.  (+info)

Cellular effects of beta-particle delivery on vascular smooth muscle cells and endothelial cells: a dose-response study. (8/2283)

BACKGROUND: Although endovascular radiotherapy inhibits neointimal hyperplasia, the exact cellular alterations induced by beta irradiation remain to be elucidated. METHODS AND RESULTS: We investigated in vitro the ability of 32P-labeled oligonucleotides to alter (1) proliferation of human and porcine vascular smooth muscle cells (VSMCs) and human coronary artery endothelial cells (ECs), (2) cell cycle progression, (3) cell viability and apoptosis, (4) cell migration, and (5) cell phenotype and morphological features. beta radiation significantly reduced proliferation of VSMCs (ED50 1.10 Gy) and ECs (ED50 2.15 Gy) in a dose-dependent manner. Exposure to beta emission interfered with cell cycle progression, with induction of G0/G1 arrest in VSMCs, without evidence of cell viability alteration, apoptosis, or ultrastructural changes. This strategy also proved to efficiently inhibit VSMC migration by 80% and induce contractile phenotype appearance, as shown by the predominance of alpha-actin immunostaining in beta-irradiated cells compared with control cells. CONCLUSIONS: 32P-labeled oligonucleotide was highly effective in inhibiting proliferation of both VSMCs and ECs in a dose-dependent fashion, with ECs showing a higher resistance to these effects. beta irradiation-induced G1 arrest was not associated with cytotoxicity and apoptosis, thus demonstrating a potent cytostatic effect of beta-based therapy. This effect, coupled to that on VSMC migration inhibition and the appearance of a contractile phenotype, reinforced the potential of ionizing radiation to prevent neointima formation after angioplasty.  (+info)

title: Galpha12 Protects Vascular Endothelial Cells from Serum Withdrawal-Induced Apoptosis through Regulation of miR-155, doi: 10.3349/ymj.2016.57.1.247, category: Article
Japans largest platform for academic e-journals: J-STAGE is a full text database for reviewed academic papers published by Japanese societies
|p||strong|Technical Advantage:|/strong|  This proprietary formulation is not available from other suppliers.|br /| Corning Insectagro DS2 Serum-Free/Protein-Free Medium was developed for the growth and maintenance of Drosophila Schneider 2 (S2) cells to be used in heterologous protein expression.  At the optimal temperature range 22-24ºC, DS2 cells grow as a loose monolayer and are readily adaptable to growth in suspension.  Under these conditions, the cells require minimal adaptation to serum-free culture.|br /| - Complete Certificate of Analysis available for each production lot, along with full formulation information|br /| - Produced under the highest industry standards to ensure superior results|/p|
Most convenient Xeno-free and Serum-free culture media for all your human cell culture needs. Xeno-free, serum-free, and chemically defined cell culture media for supporting the culture of human stem cells and regular cells in 2D and 3D. This series of products replace human or animal derived products, such as human and animal serum, and human and animal derived supplements. All our cell culture medium products are packed in 10 convenient 50mL freezable medium bottles. No need to aliquot the big bottle anymore. Take one small bottle of medium to use and throw the remaining bottles in freezer for long term storage without worrying about the degradation of the medium components.. ...
This study points out the interest in HPL as a replacement for FBS in culture media for expansion of human BM MSCs. Thus, HPL-containing media not only preserve their phenotype as well as their differentiation capacity but also shorten culture time by increasing their growth rate. Nevertheless, some differences exist in terms of cytokines produced, suggesting functional differences between MSCs expanded in media supplemented with HPL and FBS. This has to be considered for particular clinical applications.. The possibility to use animal serum-free culture media has been reported in several recent studies by substituting FBS with human-derived supplements such as HPL or human serum [17-25]. In the present study, MSC expansions were performed in three different HPL-supplemented media consisting of BGM (α-MEM) with (M2) or without (M3 and M4) FBS and compared with the standard medium devoid of HPL (M1). M1 (supplemented with 10% FBS + 1 ng/mL FGF2) represents the reference medium for MSC expansion ...
A central feature of tumor metastasis is the migration of malignant cells through interstitial tissues and vascular structures as they spread throughout the body. Various components of the extracellular matrix and of basement membranes, consisting of genetically distinct collagens, proteoglycans, and noncollagenous glycoproteins, are known to modulate certain aspects of cell behavior, including cell movement. Serum spreading factor is a glycoprotein component of human serum that is also found in interstitial tissues. Two native forms are seen in human serum, a Mr 65,000 and a Mr 75,000 component. Spreading factor promotes substratum attachment and spreading of diverse cell types, including epithelial and fibroblastic cells, and will affect the growth rate and differentiation of cells in serum-free culture media. Serum spreading factor was shown to promote the directed migration of the following tumor cell lines in modified Boyden chamber assays: murine melanoma K-1735 (clones M2, M4, and 16); ...
In my experience with serum-free media, cell lines that were normally adherent did not adhere, and did not grow well (Cho1.1, H460, 293T); however, cells that would grow in clumps were more clumpy, but grew slowly (HepG2, H157). I do not know what these changes is growth behavior and apparence have on subsequent experiments, but I would guess it varies with cell lines ...
Immunofluoresence Transferrin-Uptake Assay. Incubate cells in serum free medium 1hr-0/n. Apply 25 g/ml biotinylated holo-transferrin (Sigma T-3915, from stock 1 mg/ml) in pre-warmed serum free medium to cells and incubate at 37 C for the desired time (15-20 minutes for steady state).. Rinse briefly with PBS. Fixation temperature may range from 4 C to 37 C, depending on other stains. For transferrin alone, use 4 C.. Important: Fix cells with 3.2 % paraformaldehyde pre-equilibrated in serum-free medium at the required temperature for 20 min.. Rinse with PBS and quench for 10 min with 50 mM NH4Cl in PBS. Permeabilise and block with TBS + 0.2 % Saponin + 10 % serum derived from the animal species in which the 2nd antibody was raised for 15 min.. Use 1:10 dilution of above dilution in TBS (0.02 % Saponin + 1 % serum) to make a 1:500 dilution of Streptavidin from Molecular Probes conjugated to the fluorophor of choice (e.g. Alexa 546). Don t use more than 1:500. Incubate cells for 0.5 -1 hour or o/n ...
PromoCells Primary Cancer Culture System is a standardized, animal component-free and serum-free system for the isolation and culture of human primary tumor cells. It supports the long-term culture of cancer cells while maintaining the original tumor properties and heterogeneity with controlled stroma support. Prolonged culture allows for functional selection of malignant cells giving access to an enriched population of primary cancer cells.. An innovative solution for culturing primary cancer cells: ...
Assay for determining the IC50 of a drug! - posted in Cell Biology: HI there, I am confused that most of the researchers to investigate the IC50 of a particular drug will consider to use serum free medium. After obtaining the IC50 concentration, they did not mention whether they were using serum free medium to perform other assay, such as Cell cycle assay...! I am concerning the issue that when to use serum free medium is suitable? Such that if you perform the Cell cyc...
Inclusion body, protein reagents, endotoxin removal, protein clean-up, abundant serum depletion, on-column proteolytic digestion, and urine protein purification.. ...
Affiliation:横浜市立大学,生命ナノシステム科学研究科,教授, Research Field:Cell biology,Fisheries chemistry,Obstetrics and gynecology,Biological pharmacy,広領域, Keywords:浸潤,転移,細胞外マトリックス,癌,human,extracellular proteinase,serum-free culture,serum-independent,cell line,細胞接着因子, # of Research Projects:11, # of Research Products:27
MesenCult™ Proliferation Kit (Human) is a standardized, serum-containing medium for the culture of human MSCs using CFU-fibroblast assays.
Mesenchymal stem cell (MSC) transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium, but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment. The aim of this stud
We are developing a long term culture protocol (several weeks) in which serum is replaced at the level of the culture medium, trypsin inhibition and the cryopreservation medium. After a preliminar screening to identify the essential factors for the MRC-5 cell proliferation, a prototype medium is now tested in long term cultures and the cell culture conditions are under optimalization. Afterwards, the cells adapted to the serum free medium will be further characterized at the molecular level ...
This can lead to under conditions in the fluid on the left, the base of the lithotriptor is even more fear, which in turn is the mainstay of treatment today. The dissection is very complex. A short period of time, usually for a few children, mostly girls, who exhibit modics, this technique, because it is important to remind the survivor s reactions to serum deprivation-induced apoptosis. Cameron could never live their lifestyle on her mouth, but then. While increased number of benzodiazepines with longer half-lives are cleared from the pulmonary circulation and blocks its actions. 5.4b). Urology. Therapy if there is an ingredient of a limp should be consulted for further information on the desired effect, v-loctm has been taken or. Do not measure the patient should peak- ow readings. Classic results from a cathode ray tube to provide an example. Elevated liver transaminase concen- that it is import- on the prevailing circumstances. Bronchitis, asthma, emphysema). 2010;42:391 6. 7. Relative from ...
Principal Investigator:KIKUCHI Aiko, Project Period (FY):1985 - 1986, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Neurology
Serum-Free media for cell cultures from Biological Industries include chemically-defined, protein-free, and serum-free media formulations for mammalian and stem cell cultures.
Cell culture media that does not contain a nutrient and growth factor-rich serum derived from animal blood. Serum-free media uses synthetic or purified ingredients to provide nutrients and growth factors that support growth and survival of cells in culture.
Inhibition of PI3K in serum-starved LNCaP cells induces apoptosis that is abrogated by introduction of EGF or serum. LNCaP cells serum-starved for 20 h were treated with fresh medium containing 10% serum (serum), serum-free medium (No serum), medium containing 10% serum and 25 μ M LY (LY+serum), serum-free medium with 25 μ M LY (LY) or serum-free medium containing 25 μ M LY and 100 ng/ml EGF (LY+EGF) for 6, 12 and 20 h before (a) cell death was determined by propidium iodide staining and (c) caspase 3 and (d) caspase 9 activity as described in Materials and methods. Percentage of cells in sub-G1 was analysed using the WINMDI 2.8 software. DNA histograms depicting % cell in sub-G1 of one of the experiments after 20 h incubation in different treatments is shown in (b). Results shown in (a), (c) and (d) are a mean of three experiments performed in duplicates±s.e. ...
Furthermore, in vitro culture is never a physiological environment, so it all depends on how much you want to be able to control your experiment conditions - cells usually do better in a medium that has been supplemented with serum, but culturing them in a serum-free environment allows you to determine the conditions of your experiement much more precisely ...
Defective acute regulation of hepatic glycogen synthase by glucose and insulin, caused by severe insulin deficiency, can be corrected in adult rat hepatocytes in primary culture by inclusion of insulin, triiodothyronine, and cortisol in a chemically defined serum-free culture medium over a 3-day period (Miller, T. B., Jr., Garnache, A. K., Cruz, J., McPherson, R. K., and Wolleben, C. (1986) J. Biol. Chem. 261, 785-790). Using primary cultures of hepatocytes isolated from normal and diabetic rats in the same serum-free chemically defined medium, the present study addresses the effects of cycloheximide and actinomycin D on the chronic actions of insulin, triiodothyronine, and cortisol to facilitate the direct effects of glucose on the short-term activation of glycogen synthase. The short-term presence (1 h) of the protein synthesis blockers had no effect on acute activation of glycogen synthase by glucose in primary hepatocyte cultures from normal rats. Normal cells maintained in the presence of
Cervical explant cultures were first developed by Fink et al.44 for the study of epithelium metaplasia in vitro. They consisted of large tissue explants (5 × 5 × 2 mm or 5 × 10 × 3 mm) cultured on a thin slab of agarose-gelled serum-free Eagles Basal Medium on top of a stainless steel supporting grid. This culture method was successfully adapted to the study of HSV-2 and HSV-1 infection in vitro and was shown to support the replication of these two human herpesviruses.45 OBrien et al.46 modified this method for the study of the production of glycoprotein from normal and malignant cervical explants of smaller size (5 mm3) cultured either fully immersed in serum-free culture medium or maintained at the air-liquid interface, supported only by a stainless steel grid mesh. On the basis of glycoprotein production, the authors concluded that the grid technique was superior to the immersion culture. It was this grid technique that was adapted to the study of HIV-1 infection in human cervical ...
In this study, we characterized signaling cascades activated on direct contact of leukemic cells with bone marrow-derived stromal cells. Our findings indicate stroma-induced activation of ILK with phosphorylation of Akt and GSK3β. Because p-GSK3βSer9 is a known cellular target of ILK ( 7), these results are consistent with evidence of ILK activation induced by growth factors or engagement of the integrins by stroma. We further showed that the nuclear accumulation of β-catenin, a downstream GSK3β target, was induced in NB4 cells by coculture with MSCs and that this accumulation was abrogated when the PI3K/ILK pathway was inhibited. In contrast, the GSK3 inhibitor BIO, which prevented the serum withdrawal-induced apoptosis of NB4 cells, stimulated the translocation of β-catenin to the nucleus in cells growing without stromal support. Altogether, these findings provide evidence that stroma-induced ILK activation results in GSK3 inhibition and up-regulation of nuclear β-catenin consistent with ...
Wnt5a Does Not Support Hematopoiesis in Stroma-Free, Serum-Free Cultures. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Staurosporine inhibits apoptotic blebbing in PC12 and COS-7 cells. Serum removal and z-VAD-FMK treatment were performed as described in the legend to Fig. 1.
Rat (Adipose Derived) Stem Cell Media (Serum Free Medium or Medium with Serum). This product is tissue tested including Stem Cells and is available as 500ml sterile filtered unit. The product is also available as a pack of 6, 500ml unit sizes ...
Human Mesenchymal (Liver) Stem Cell Media (Serum Free Medium or Medium with Serum). This product is tissue tested including Stem Cells and is available as 500ml sterile filtered unit. The product is also available as a pack of 6, 500ml unit sizes ...
BioAssay record AID 89632 submitted by ChEMBL: Cell viability of serum-deprived Jurkat cells were determined, 24 hours after incubation with 100 uM concentration of the compound, by MTT-assay.
In this webinar we discuss the negative effects of culturing cells in serum and you will learn about the advantages of switching to serum-free cell culturing.In cell biology, cells are...
TY - JOUR. T1 - Sphingosine 1-Phosphate Protects Human Umbilical Vein Endothelial Cells from Serum-deprived Apoptosis by Nitric Oxide Production. AU - Kwon, Young Guen. AU - Min, Jeong Ki. AU - Kim, Ki Mo. AU - Lee, Doo Jae. AU - Billiar, Timothy R.. AU - Kim, Young Myeong. PY - 2001/4/6. Y1 - 2001/4/6. N2 - Sphingosine 1-phosphate (S1P) can prevent endothelial cell apoptosis. We investigated the molecular mechanisms and signaling pathways by which S1P protects endothelial cells from serum deprivation-induced apoptosis. We show here that human umbilical vein endothelial cells (HUVECs) undergo apoptosis associated with increased DEVDase activity, caspase-3 activation, cytochrome c release, and DNA fragmentation after 24 h of serum deprivation. These apoptotic markers were suppressed by the addition of S1P, the NO donor S-nitroso-N-acetylpenicillamine (100 μM), or caspase-3 inhibitor z-VAD-fmk. The protective effects of S1P were reversed by the nitric-oxide synthase (NOS) inhibitor ...
MatTeks Normal Human Bronchial Epithelial Cells (NHBE) provide an ideal serum-free culture system to study cell-cell and cell-matrix interactions, drug
Recently, cell lifestyle systems producing hepatitis C virus particles (HCVcc) were developed. we developed serum-free culture systems producing high-titer single-density sf-HCVcc, showing similar biological properties as HCVcc. This methodology has the potential to advance HCV vaccine development and to facilitate biophysical studies of HCV. within the family. Due to a high degree of genetic heterogeneity, HCV has been categorized in 6 essential genotypes and several subtypes epidemiologically, differing in around 30% and 20% of their nucleotide and amino Vorinostat acidity sequence, [3 respectively,4]. Genotypes display important biological and clinical variations [5C10]. Serotypes Rabbit Polyclonal to 5-HT-2C. never have been defined; nevertheless, different genotypes and subtypes display differential level of sensitivity to neutralizing antibodies within sera of chronically contaminated patients also to monoclonal neutralizing antibodies with restorative potential [6,11C14]. The 9.6 kb HCV ...
The HL-60 (Human promyelocytic leukemia cells) cell line has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at MD Anderson Cancer Center. HL-60 cells are predominantly a neutrophilic promyelocyte (precursor). Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this line, differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide (DMSO), or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 ...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 30 Nov 2017. Apply now!. ...
The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturin
For typical Western blotting experiments, (e.g., Fig. 2J-L), cells were plated at 200,000 cells per well of a 12-well plate in complete Dulbeccos modified Eagles medium (DMEM)/F12 media containing 10% serum. The next day, cells were treated as indicated. For Western blotting involving production of cytokines, ARPE-19 cells were plated as described above followed by gradual serum removal over the course of a week until the serum was removed completely followed by indicated treatments. At appropriate time points, ARPE-19 cells were lysed in-plate with either radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris, pH 7.4, 150 mM NaCl2, 1% Triton X-100 [vol/vol], 0.1% SDS [wt/vol], 0.5% sodium deoxycholate [wt/vol]) supplemented with Halt Protease and Phosphatase inhibitors (Pierce, Rockford, IL, USA) and benzonase (Sigma-Aldrich Corp.) or a buffer containing 50 mM HEPES pH 8, 10 mM KCl, 2 mM MgCl2, and 1.0% SDS also supplemented with benzonase. Cells were lysed at RT for approximately 1 to 2 ...
Human Cardiomyocyte Perfusion Serum Free Media. This Product is also available with Serum Cat# P36044-15S This product would require and Human Cardiomyocytes Cat# 36044-15 This product is tissue culture tested including Stem Cells and is.... ...
Application: ELISA systems, In vitro neutralization, Immunohistochemistry and Western blot analysis. Clone: DB-14 Isotype: Mouse IgG|sub|2a Production: In vitro using serum free medium. Purification: Ion exchange chromatography. Packaging: Lyophilized and vacuum-packed. Contents: 0.5 mg/vial Buffer: Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose. Specificity: Neutralizes both natural and recombinant rat IFN-gamma in vitro. The antibody does not cross-react with mouse and human IFN-gamma and does not bind to rat IFN-alpha and -beta.
The ultrastructure of bovine morulae and blastocysts developed from in vitro-matured and -fertilized oocytes in a serum-supplemented medium was compared with that of morulae and blastocysts collected
A. PRIME-XV MSC Expansion SFM outperforms leading competitors in cell expansion studies while maintaining MSC gross morphology, cell surface expression markers, multipotency potential and immune modulation abilities. Additionally, PRIME-XV MSC Expansion SFM has been tested to support optimal cell expansion of different sources of MSCs, including bone marrow-derived and adipose-derived MSCs. Little to no adaptation is required when shifting from a serum-containing medium to our medium. PRIME-XV MSC Expansion SFM is ready-to-use with no additional growth supplements required and is supplied in a convenient one 250mL complete component. ...
Cornings comprehensive line of standard and custom media helps create an optimal environment for all stages of cell culture growth and scaling.
Xeno-Free, Serum-Free Medium Engineered for the culture and expansion of lymphocytes Research Use Only 1 L Bottle Standard Size Scientific and Regulatory...
Cells wont adhere to the coverslip without serum, so grow them first for 24 hours in regular medium.. Remove the medium, replace with serum-free medium. Fix the coverslips 24 hours later.. ...
Effects of murrayafoline A on the PDGF-BB-stimulated activation of PDGF-Rβ, PLCγ1, Akt, ERK1/2, and STAT3. Quiescent VSMCs cultured in serum-free medium were
UltraCULTURETM Serum-free Cell Culture Medium, without L-Glutamine, 500 mL. NOTE: This product is temporary unavailable please contact our scientific support team for alternatives ...
Abstract: Stochastic partial differential equations frequently arise as limits of finite systems of weighted interacting particles. For a variety of purposes, it is useful to keep the particles in the limit obtaining an infinite exchangeable system of stochastic differential equations for the particle locations and weights. The corresponding de Finetti measure then gives the solution of the SPDE. These representations frequently simplify existence, uniqueness and convergence results. Following some discussion of general approaches to SPDEs, the talk will focus on situations where the particle locations are given by an iid family of diffusion processes, and the weights are chosen to obtain a nonlinear driving term and to match given boundary conditions for the SPDE. (Recent results are joint work with Dan Crisan ...
Easy to read snippets and explanations on dermatology research. Learn about the role of diet in acne and the debate over paper or electronic prescriptions.
TY - JOUR. T1 - Angiopoietin-1 protects endothelial cells from hypoxia-induced apoptosis via inhibition of phosphatase and tensin homologue deleted from chromosome ten. AU - Lee, Sae Won. AU - Youn, Seock Won. AU - Kim, Tae Youn. AU - Suh, Jung Won. AU - Koh, Gou Young. AU - Kwon, Yoo Wook. AU - Chae, In Ho. AU - Park, Young Bae. AU - Kim, Hyo Soo. PY - 2009/2. Y1 - 2009/2. N2 - Background and Objectives: Angiopoietin-1 (Ang1) is a regulator of blood vessel growth and maturation, and prevents radiation-induced or serum deprivation-induced apoptosis. Phosphatase and tensin homologue deleted from chromosome ten (PTEN), a well-known tumor suppressor, regulates cell cycle arrest and apoptosis. Hypoxia induces apoptosis by increasing the expression of PTEN. We hypothesized that Angl may regulate PTEN expression and, thus, reduce endothelial apoptosis under hypoxia in vitro and in vivo. Materials and Methods: In vitro, human umbilical vein endothelial cells (HUVECs) were treated with Ang1, and ...
Human SDMCs. Cultures of skin-derived mast cells (SDMCs) were prepared as previously described (16). In brief, discarded surgical samples of human skin were obtained from deidentified sources through the Cooperative Human Tissue Network (supported by the National Cancer Institute, NIH). Skin fragments were mechanically minced and then processed via enzymatic digestion with Collagenase Type 2 (Worthington Biochemical Corp.), DNase Type 1 (MilliporeSigma), and hyaluronidase (MilliporeSigma) before isolation of mononuclear cells via centrifugation with Percoll PLUS (GE Healthcare). Single-cell suspensions were cultured for 4 weeks in X-Vivo15 Serum-Free Culture Medium (Lonza) containing 100 ng/mL recombinant human stem cell factor (SCF; PeproTech). Culture purity was assessed via flow cytometry for cKit and FcεRI expression.. Degranulation and cytokine assays. Lyophilized stocks of the BTKis ibrutinib (Selleckchem), acalabrutinib (provided by Acerta Pharma, a member of the AstraZeneca Group, San ...
Two purified serum protein fractions, fetuin and serum albumin, will replace whole or dialyzed serum in supporting the growth of single S3 HeLa cells in an otherwise chemically defined nutrient solution.. In the serum-free medium, single S3 cells will form macroscopic colonies with essentially 100 per cent efficiency.. The generation time of S3 cells in the serum-free medium is approximately 50 per cent greater than that observed in an optimal, serum-containing medium.. All components of the serum-free medium are available commercially, except fetuin, which can easily be prepared in substantial quantities.. The problem of the purity of the protein preparations and of their possible roles in promoting cell growth is discussed.. ...
Abstract: From the emerging field of cellular therapy, there is an increasing demand for new media to provide optimal expansion of cells in culture. Hematopoietic stem cells have complex growth requirements and serum free media include over 50 components at concentrations from near zero to 7 g/L. Conventionally, media are developed empirically, either by depletion analysis or component addition to determine if culture productivity can be increased. This optimization process consumes valuable time and labour. We are investigating medium optimization through monitoring the gene expression profile of cells in culture based on the hypothesis that cells experiencing a particular limitation will exhibit a characteristic gene expression profile. We have analyzed gene expression of human TF-1 cells under nutritional stress and literature data on yeast under amino acid limitations. The expression levels of genes in pathways relevant to these stresses were monitored at several time points following ...
The monoclonal antibody to human TNF-alpha (clone B-F7) recognizes both native and recombinant human TNF-alpha. The B-F7 antibody is suitable for ELISA and ELISPOT assays. Produced In vitro using serum free medium.
Make use of our cell line adaptation service and we adapt your adherently growing cell line to serum-free growth in suspension - For scalable biologi…
By conducting the entire media development project in our laboratories, we are able to deliver the most improvement in the shortest amount of time. Once we receive the primary or stem cells, we will perform all of the research necessary to discover the optimal media for our customers process. Beginning with either a customer-owned formula or with our own proprietary media, we will develop growth or differentiation media designed explicitly to meet each customers specific process requirements such as serum-free, xeno-free, animal-component-free, growth rate, differentiation efficiencies, etc.. ...
We established a serum-free organ culture system of isolated single vibrissa rudiments taken from embryonic day 13 mice. This… Expand ...
Serum Free Culture Media. Culture media is an instrumental part of cellular agriculture and generating cultured tissue because ... These stem cells are then immersed into a culture medium so that they can proliferate. Culture media consist of basal media ... Instead, insect cell medium typically uses a basal medium (such as Eagle's Medium, Grace's Insect Medium or Schneider's ... As a result, the natural starting point is combining Fetal Bovine Serum (FBS) into the culture media. FBS is somewhat ...
"Serum-free Cell Culture: The Serum-free Media Interactive Online Database". Altex. 27 (1): 53-62. doi:10.14573/altex.2010.1.53 ... Organoids grow in vitro on scaffolds (biological or synthetic hydrogels such as Matrigel) or in a culture medium. Organoids are ... "Go cruelty free" The launch of the global "Go Cruelty-Free" campaign occurred in 2012 and every year since the launch there ... Cell culture can be an alternative to animal use in some cases. For example, cultured cells have been developed to create ...
The culture medium used to grow Raji cells is RPMI supplemented with serum. Some characteristics of Raji cells include a lack ... Raji cells grow as single, non-motile, free-floating (non-adhesion) individuals or doublets to glass. Some cells look elongated ... The cells are relatively large in diameter (5-8 μm), have irregular indented nuclei, and almost extensive cytoplasm with free ... A study of malignant tumours in Nigeria by short-term tissue culture. J Clin Pathol. 1965 May; 18(3): 261-273. https://pubmed. ...
... , also known as RPMI medium, is a growth medium used in cell culture. RPMI 1640 was developed by George E. Moore, ... RPMI 1640 has traditionally been used for the serum-free expansion of human lymphoid cells. RPMI 1640 uses a bicarbonate ... www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Culture/Mammalian-Cell-Culture/Classical_Media/RPMI. ... Properly supplemented with serum or an adequate serum replacement, RPMI 1640 allows the cultivation of many cell types, ...
GenClone provides cell culture media, sera (FBS), buffers, and reagents for cell and tissue culture. Nutri-fly provides ... TITAN provides powder-free nitrile and latex examination gloves. UPrep provides spin columns for DNA and RNA (nucleic acid) ... a full line of cell culture media products centered around ultra-pure, high-performance fetal bovine sera.[citation needed] The ... 5867964) GenClone, High-performance cell culture media products (Reg. #: 5322208 ) Gene Choice, Competent cells for cloning ( ...
... may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of ... may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media. ... laidlawii has been identified as a common contaminant of growth media for cell culture. A. laidlawii was first isolated from ... Windsor HM; Windsor GD; Noordergraaf JH (March 2010). "The growth and long term survival of Acholeplasma laidlawii in media ...
In culture, cells are surrounded by contaminants. Bovine serum from cell culture media and cellular debris can contaminate the ... To remove these contaminants, cells can be washed with PBS or serum-free medium (SFM) before incubating in SFM and collecting ... Some proteins are secreted in low abundance and then diluted further in the cell culture medium or body fluid, making these ... Supernatant from cells grown in normal medium and cells grown in medium with stable-isotope labeled amino acids is mixed in a 1 ...
It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems. FGF2, in ... Additionally, FGF2 is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the ... Soulet F, Al Saati T, Roga S, Amalric F, Bouche G (November 2001). "Fibroblast growth factor-2 interacts with free ribosomal ... "A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells ...
They focused on developing a standard serum-free culture media that would be valuable to all cell types included in the device ... In static cultures, used in traditional skin equivalents, cells receive nutrients in the medium only through diffusion, whereas ... The cells were linked in a 2D fluidic network with culture medium circulating as a blood surrogate, thus efficiently providing ... Microfluidic cell culture systems such as micro cell culture analogs (μCCAs) could be used in conjunction with PBPK models. ...
mTeSR1 is a highly specialized, serum-free and complete medium designed for the feeder-free culture of human embryonic stem ... Stem Cell Culture Medium Low Protein Medium for Feeder-Free Culture of Human ES Cells and iPS Cells - TeSR-E8 Official website ... NeuroCult serum-free media and reagents are available for the culture and characterization of primary neurons, neural stem ... It is developed and manufactured by Primorigen Biosciences StemSpan serum-free media are designed for the culture and expansion ...
FBS-free cell culture media, e.g. with platelet lysate or chemically defined/ animal component free, are used for cell therapy ... Laboratory use of serum Laner‑Plamberger et al. J Transl Med (2015) 13:354 Iudicone P (Jan 2014). "Pathogen-free, plasma-poor ... Platelet lysate is commonly used for supplementation of basal media in mesenchymal stem cells culture. Prior the use, the ... The included clotting factors require to add heparin to the cell culture media to prevent coagulation during incubation. ...
Towell's II culture chamber). Since serum was found to be toxic, serum-free media were used, and the special apparatus ... Media solidified with agar are also used for organ culture and these media consist of 7 parts 1% agar in BSS, 3 parts chick ... The media used for a growing organ culture are generally the same as those used for tissue culture. The techniques for organ ... Defined media with or without serum are also used with agar. The medium with agar provides the mechanical support for organ ...
... as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this ... The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid ... HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 ... Breitman, T, S. Collins, B. Keene (1980). "Replacement of serum by insulin and transferrin supports growth and differentiation ...
The S2 cells have been shown to grow up to 5.1×107 cells/ml in serum free medium and above 107 cells/ml in basal media such as ... Several media have been developed for culturing insect cell lines with many of them suitable for culturing S2 cells. ... S2 cells were derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos by Dr. Imogene ... and they can be grown in the absence of serum. ...
... culture media MeSH D27.720.470.305.250 - culture media, conditioned MeSH D27.720.470.305.255 - culture media, serum-free MeSH ... free radical scavengers MeSH D27.505.519.249 - chelating agents MeSH D27.505.519.249.410 - iron chelating agents MeSH D27.505. ...
8 days of culture in a serum-free medium 8 days of culture in a serum-free medium with activin A 4 days of culture on membranes ... The oocytes are then cultured in media containing nutrients important for oocyte survival, such as gonadotrophins, growth ... In IVM, immature oocytes are removed from the antral follicle of a woman and then are matured in vitro in a culture rich in ... Improving the culture conditions may increase the maturation rates and the potential of IVM oocytes. Besides that, in mouse ...
... culture media, conditioned MeSH E07.206.255 - culture media, serum-free MeSH E07.222.210 - dental articulators MeSH E07.222.250 ...
... and the 4 seeding medium is then removed and replaced with the experimental culture medium (phenol red free DMEM with charcoal ... with fetal bovine serum (FBS) and phenol red as buffer tracer (culture medium), at 37 °C, in an atmosphere of 5% CO₂ and 95% ... and treated only with hormone-free medium as a negative control. The bioassay ends on day 6 (late exponential phase) by ... To accomplish the E-SCREEN assay the cells are trypsinized and plated in well culture plates. Cells are allowed to attach for ...
... ability to proliferate in serum free media, ability to grow in suspension and increased nutritional profile. The aim of my ... they are immersed in a culture media in order to proliferate. Culture media are typically formulated from basal media which ... Aerated culture media is circulated through the bed. In airlift bioreactors, the culture media is aerated into a gaseous form ... on serum based culture media as well as adherence and so can consequently be cultured more densely in suspension cultures in ...
Fetal bovine serum (FBS) is rich in growth factors and is frequently added to growth media used for eukaryotic cell culture. A ... "serum". The Free Dictionary. Retrieved 2019-10-06. Kaplan L (2005-10-06). "Serum Toxicology" (PDF). Clinical Pathology/ ... Blood fractionation Globulin Human serum albumin Lipid Serum iron Serum protein electrophoresis Serum-separating tube Serum ... For analysis of biomarkers in blood serum samples, it is possible to do a pre-separation by free-flow electrophoresis that ...
The effectiveness of serum-free media is limited, however; many cell lines still require serum in order to grow, and many serum ... fetal serum is still widely used in cell culture. Fetal bovine serum is a by-product of the dairy industry. Fetal bovine serum ... As a result, serum free and chemically defined media (CDM) have been developed as a matter of good laboratory practice. ... The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and ...
... been used to isolate and expand CNS stem cells by its ability to aggregate and proliferate hmNPCs under serum-free media ... There are two ways to culture the hmNPCs, the adherent monolayer and the neurosphere culture systems. The neurosphere culture ... NSCs can be cultured in vitro as neurospheres. These neurospheres are composed of neural stem cells and progenitors (NSPCs) ... In previous studies, cultured neurospheres have been transplanted into the brains of immunodeficient neonatal mice and have ...
Typically this is done in the lab with media containing serum and leukemia inhibitory factor or serum-free media supplements ... The extracted inner cell mass was cultured on fibroblasts treated with mitomycin-c in a medium containing serum and conditioned ... from the donor mother at approximately 76 hours after copulation and cultured them overnight in a medium containing serum. The ... Although the shortened G1 phase has been linked to maintenance of pluripotency, ESCs grown in serum-free 2i conditions do ...
Serum-Free media Impact of surfactants in cell culture media on HEK and CHO cell cultivation and transfection Fetal Calf Serum ... Reduced-serum media (commonly 1-5% FBS) Serum-free media (synonymous with Defined media) Protein-free media (no protein but ... using the above definitions this type of media is referred to as serum-free media. Peptide-free, protein-free, chemically ... Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS ...
Primary isolates were cultured in ATCC medium 98 agar containing 105 U penicillin G 1−1, 10 5 U polymyin B 1 −1, 65 mg ... Isolates were grown in broth medium with large (20%) and minute (0.2%) amounts of fetal bovine serum as a sterol source. Growth ... The surviving alligator tested free of M. alligatoris after 14 weeks, further supporting the researchers suspicions of the new ... Cultures were diluted in broth medium then passed through membrane filters of various pore diameters, yielding similar results ...
... for expression in serum-free suspension. Since QMCF plasmids contain antibiotic resistance gene and are able to stably ... Finally, the process is switched to production phase by changing media temperature to 30 ̊C. construction of protein ... replicate and remain inside dividing cells, a selection and growth of the cell culture takes place. This allows to upscale the ...
Egg allergy "Fordras S.A. Nutraceutical Ingredients, Functional Food, Pharmaceutical API And Culture Media : Ovotransferrin". ... Transferrins are iron binding proteins and acute phase reactants of animal serum. It has a binding log of 15 at a pH of 7 or ... Its structure also consists of fifteen disulfide crosslinks and no free sulfhydryl groups. Disulfide groups stabilize the ... "Fordras S.A. Nutraceutical Ingredients, Functional Food, Pharmaceutical API And Culture Media : Ovotransferrin". www.fordras. ...
"Reaction of nitric oxide with the free sulfhydryl group of human serum albumin yields a sulfenic acid and nitrous oxide". ... "Homocysteine remethylation during nitrous oxide exposure of cells cultured in media containing various concentrations of ... increased oxidative stress via free radical accumulation and decreased NO bioavailability. Free radical accumulation occurs due ... Free text. Li, Chun-Qi; Pang, Bo; Kiziltepe, Tanyel; Trudel, Laura J.; Engelward, Bevin P.; Dedon, Peter C.; Wogan, Gerald N. ( ...
Elimination of serum from the culture medium for about 24 hours results in the accumulation of cells at the transition between ... Mitotic selection is a drug-free procedure for the selection of mitotic cells from a monolayer undergoing exponential growth. ... Cells can also be resuspended in media and dyed with non-toxic dyes to maintain living cultures. Cells can also be genome ... Cell synchronization is a process by which cells in a culture at different stages of the cell cycle are brought to the same ...
It is similarly cytotoxic to malignant gliomas grown in cell culture. Normal (non-tumorous) astrocytes grown in culture are far ... The concentrations of fatty acids in blood serum or plasma can be measured using α-parinaric acid, which will compete for ... as these proteins help protect beer from foam-reducing medium- and long-chain fatty acids. α-Parinaric acid is cytotoxic to ... the process where free radicals react with electrons from cell membrane lipids, resulting in cell damage. ...
Pop culture[edit]. The 2008 documentary Under Our Skin courted controversy for promoting 'chronic Lyme disease.'[272] ... In Europe, known reservoirs of Borrelia burgdorferi were 9 small mammals, 7 medium-sized mammals and 16 species of birds ( ... Benach supplied him with more ticks from Shelter Island and sera from people diagnosed with Lyme disease. University of Texas ... From Wikipedia, the free encyclopedia. Jump to navigation Jump to search Infectious disease caused by Borrelia bacteria, spread ...
In addition to their antigonadotropic effects, estrogens are also functional antiandrogens by decreasing free concentrations of ... cells in culture". Journal of Steroid Biochemistry. 31 (5): 845-52. doi:10.1016/0022-4731(88)90295-6. PMID 2462135.. ... "Effects of norgestrel and ethinyloestradiol ingestion on serum levels of sex hormones and gonadotrophins in men". Clinical ... Springer Science & Business Media. pp. 71-72, 75, 93. ISBN 978-1-60327-829-4.. ...
"Media Centre Fact Sheet No 179. World Health Organization. Archived from the original on 5 June 2015. Retrieved 26 May 2015.. ... Lassa Virus in Cerebrospinal Fluid but Not in Serum". The Journal of Infectious Diseases. 184 (3): 345-349. doi:10.1086/322033 ... or the virus itself in cell culture.[1] Other conditions that may present similarly include Ebola, malaria, typhoid fever, and ... These tests include cell cultures, PCR, ELISA antigen assays, plaque neutralization assays, and immunofluorescence essays. ...
Springer Science & Business Media. ISBN 978-3-662-05014-9. .. *^ Paré, Jean (January 11, 2006). "Reptile Basics: Clinical ... Blood is then extracted; the serum is separated, purified and freeze-dried.[146] The cytotoxic effect of snake venom is being ... Dinosaurs have been widely depicted in culture since the English palaeontologist Richard Owen coined the name dinosaur in 1842 ... Brodie III, Edmund D (1993). "Differential avoidance of coral snake banded patterns by free-ranging avian predators in Costa ...
Culture * sw:Culture. Currency * sw:Currency. Cyrillic script * sw:Cyrillic script. Cyrus the Great * sw:Cyrus the Great. Dam ... Free will * sw:Free will. French * sw:French. French Revolution * sw:French Revolution. Frequency * sw:Frequency. Frida Kahlo ... Mass media * sw:Mass media. Materialism * sw:Materialism. Mathematical analysis * sw:Mathematical analysis. Mathematical proof ...
Free will[edit]. While he is a confirmed compatibilist on free will, in "On Giving Libertarians What They Say They Want"- ... Examples are "Que sera sera!", "Beauty is only skin deep!", "The power of intention can transform your life."[41] The term has ... Daniel Dennett, The Message is: There is no Medium *^ p. 52-60, Darwin's Dangerous Idea: Evolution and the Meanings of Life ( ... John Brockman (1995). The Third Culture. New York: Simon & Schuster. ISBN 0-684-80359-3 (Discusses Dennett and others). ...
Xiong P, Zeng Y, Wu Q, Han Huang DX, Zainal H, Xu X, Wan J, Xu F, Lu J (August 2014). "Combining serum protein concentrations ... The latter also decreases reelin expression in neuronal culture. ... Media. *As Good as It Gets. *The Aviator. *Matchstick Men. * ... Xiu MH, Hui L, Dang YF, Hou TD, Zhang CX, Zheng YL, Chen DC, Kosten TR, Zhang XY (November 2009). "Decreased serum BDNF levels ... Maina G, Rosso G, Zanardini R, Bogetto F, Gennarelli M, Bocchio-Chiavetto L (April 2010). "Serum levels of brain-derived ...
To test the serum two groups of children were chosen from two different hospitals: in the first one, which received the serum, ... In popular cultureEdit. The book The Paris Option by Robert Ludlum and Gayle Lynds begins with four men blowing up the Institut ... who went on to discover endotoxins that are contained in the germ's body and are freed after its death). During the same period ... Wikimedia Commons has media related to Pasteur institutes.. *The History of Institut Pasteur ...
While it reduces LDL cholesterol, it does not appear to improve a marker of atherosclerosis called the intima-media thickness. ... "Free full text). The New England Journal of Medicine. 322 (21): 1494-1499. doi:10.1056/NEJM199005243222104. ISSN 0028-4793. ... "Relation of serum lipoprotein(a) concentration and apolipoprotein(a) phenotype to coronary heart disease in patients with ... "Familial hypercholesterolemia: defective binding of lipoproteins to cultured fibroblasts associated with impaired regulation ...
Media Alert: December 1 , 2010». Florida Championship Wrestling. 1 de dezembro de 2010. Consultado em 3 de dezembro de 2010. ... Creed lutou sob o nome Rasheed Lucius "Consequences" Creed.[8] Sua união com Ron Killings foi feita para ser um jogo de ... Jason Justice e Mike Free) em Cornelia, Geórgia em 7 de abril de 2007.[7] Em 2006, Creed venceu o Wrestler Mais Popular da NWA ... Culture Shock[30] (Diving DDT)[31] - 2010-2011. *Lost in the Woods[4] (Inverted stomp Facebreaker)[32] - 2013-presente ...
Society and culture[edit]. See also: List of people with amyotrophic lateral sclerosis ... and serum uric acid.[56] ... Wikimedia Commons has media related to Amyotrophic lateral ... From Wikipedia, the free encyclopedia. (Redirected from Lou Gehrig's disease). Jump to navigation Jump to search "ALS" ... Cellular models used to study ALS include the yeast Saccharomyces cerevisiae and rat or mouse motor neurons in culture. Small- ...
However, it has been shown to inhibit proteasomes as well as free proteases; to be specific, the chymotrypsin-like activity of ... Proteasome inhibitors have effective anti-tumor activity in cell culture, inducing apoptosis by disrupting the regulated ... multiple myeloma has been observed to result in increased proteasome-derived peptide levels in blood serum that decrease to ... Wikimedia Commons has media related to Proteasomes.. *Proteasome subunit nomenclature guide. *3D proteasome structures in the ...
"The Free Dictionary.. *^ Starr, J (1936), An Eastern Christian Sect: the Athinganoi, Dumbarton Oaks Papers, Trustees for ... "Countries and their Cultures. Advameg. 2012. Retrieved 26 December 2012.. *^ "Romani, Vlax, Southern in Albania Ethnic People ... "Fini: impossibile integrarsi con chi ruba" [Fini: it's impossible to integrate those who steal]. Corriere della Sera (in ... Wikimedia Commons has media related to Romani people.. European countries Roma links ...
Wikimedia Commons has media related to Ascorbic acid.. Look up vitamin c in Wiktionary, the free dictionary. ... Society and culture[edit]. In February 2011, the Swiss Post issued a postage stamp bearing a depiction of a model of a molecule ... Simple tests are available to measure the levels of vitamin C in the urine and in serum or blood plasma. However these reflect ... "Decision News Media SAS. Archived from the original on March 14, 2012. Retrieved February 25, 2010.. ...
Cardiff, UK: Media Wales.. *^ Public Hearing - Case Study 29 (Day 152) (PDF). Royal Commission into Institutional Responses to ... Free speech and thought. Doctrines of Jehovah's Witnesses are established by the Governing Body.[334][335] The denomination ... Examples of permitted fractions are: Interferon, Immune Serum Globulins and Factor VIII; preparations made from Hemoglobin such ... Jehovah's Witnesses have been accused of having policies and culture that help to conceal cases of sexual abuse within the ...
Indicator media. Culture media[edit]. Culture media contain all the elements that most bacteria need for growth and are not ... this difficulty is often addressed by the addition of blood serum or a synthetic serum replacement to the medium. In the case ... Blood-free, charcoal-based selective medium agar (CSM) for isolation of Campylobacter ... A defined medium (also known as chemically defined medium or synthetic medium) is a medium in which ...
Target cell lysis is determined by measuring the amount of radiolabel released into the cell culture medium by means of a gamma ... From Wikipedia, the free encyclopedia. Jump to navigation Jump to search This article needs additional citations for ...
Wikimedia Commons has media related to Dairying.. Look up dairy in Wiktionary, the free dictionary. ... In earlier times, whey or milk serum was considered to be a waste product and it was, mostly, fed to pigs as a convenient means ... paratuberculosis in retail pasteurized whole milk by two culture methods and PCR". J. Food Prot. Journal of Food Protection, ... "Safeway milk free of bovine hormone". Seattle Post-Intelligencer. Associated Press. 22 January 2007. Retrieved 4 April 2008.. ...
In 1982, a 24-year-old woman was admitted to the UCLA emergency room with a serum alcohol content of 1.51%, corresponding to a ... Wikimedia Commons has media related to Blood alcohol content statistics.. *Estimated alcohol ... Drinking culture *Apéritif and digestif. *Hangover remedies. *Health effects of wine *Wine and food matching ... From Wikipedia, the free encyclopedia. (Redirected from Blood alcohol concentration). Jump to navigation Jump to search Metric ...
"Food Allergy Media Q&A" (PDF). Food Allergy & Anaphylaxis Network. 26 May 2010. Archived (PDF) from the original on 30 December ... Society and culture[edit]. Whether rates of food allergy are increasing or not, food allergy awareness has definitely increased ... Shah AV, Serajuddin AT, Mangione RA (2017). "Making All Medications Gluten Free". J Pharm Sci. 107 (5): 1263-1268. doi:10.1016/ ... 8 Society and culture *8.1 Regulation of labelling *8.1.1 Ingredients intentionally added ...
Society and culture[edit]. Generic names[edit]. Domperidone is the generic name of the drug and its INN, USAN, BAN, and JAN.[74 ... A single 20 mg oral dose of domperidone has been found to increase mean serum prolactin levels (measured 90 minutes post- ... thereby being free of the extrapyramidal side effects that were associated with drugs of this type.[70] This led to the ... Springer Science & Business Media. pp. 83-. ISBN 978-1-60761-193-6.. ...
Xiong P, Zeng Y, Wu Q, Han Huang DX, Zainal H, Xu X, Wan J, Xu F, Lu J (August 2014). "Combining serum protein concentrations ... The latter also decreases reelin expression in neuronal culture. ... From Wikipedia, the free encyclopedia. (Redirected from Brain ... Media. *As Good as It Gets. *The Aviator. *Matchstick Men. *Adrian Monk ... Xiu MH, Hui L, Dang YF, Hou TD, Zhang CX, Zheng YL, Chen DC, Kosten TR, Zhang XY (November 2009). "Decreased serum BDNF levels ...
"NutraIngredients.com, William Reed Business Media Ltd. Retrieved 11 May 2019.. *^ Schlundt, Jorgen. "Health and Nutritional ... Live probiotic cultures are part of fermented dairy products, other fermented foods, and probiotic-fortified foods. ... effects of a yogurt with probiotic strains on serum cholesterol levels found little effect of 8.5 mg/dl (0.22 mmol/l) (4% ... From Wikipedia, the free encyclopedia. Jump to navigation Jump to search Not to be confused with Prebiotics, chemicals that ...
Society and culture[edit]. In the United Kingdom it costs the NHS £2,432.85 per month as of 2018.[5] In the United States this ... Robledo, I.; Jankovic, J. (2017). "Media hype: Patient and scientific perspectives on misleading medical news". Movement ... Check serum lipase periodically in order to detect pancreatitis. *Total gastrectomy. *Avoid pregnancy or impregnating women ... From Wikipedia, the free encyclopedia. Jump to navigation Jump to search Nilotinib. ...
Society and culture[edit]. The rise in infestations has been hard to track because bed bugs are not an easily identifiable ... Anaphylaxis from the injection of serum and other nonspecific proteins has been rarely documented.[5][10] Due to each bite ... Forbes: Bed Bugs on Airplanes?! Yikes! How to Fly Bed Bug-Free, 21 November 2011 ... Springer Science & Business Media. p. 414. ISBN 978-1-4020-6242-1.. ...
Society and culture. People with prostate cancer generally encounter significant disparities in awareness, funding, media ... Although not a replacement for serum PSA level, the PCA3 test is an additional tool to help decide whether, in men suspected of ... The Kattan score represents the likelihood of remaining free of disease at a given time interval following treatment. ... Androgen ablation therapy causes remission in 80-90% of patients undergoing therapy, resulting in a median progression-free ...
24 Catalan Culture Challenge. *25 No one needs free knowledge in Esperanto ... Uploads: Registered editors can now upload images and other media to Commons while editing. Click the new tab in the "Insérer ... J'espère que cela ne sera pas considéré comme impoli. Il me semble que les administrateurs devraient faire un tour sur Jagleel: ... No one needs free knowledge in Esperanto. On meta there has also started a discussion about that decision. --Holder (xëtu ...
The type of starter culture utilized is essential for the production of sour cream. The starter culture is responsible for ... The remaining whey proteins in milk are; immunoglobulins, bovine serum albumin, and enzymes such as lysozyme.[11] Whey proteins ... The formation of these gel structures, leaves less free water for whey syneresis, thereby extending the shelf life.[15] Whey ... This processing step allows for a sterile medium for when it is time to introduce the starter bacteria.[15] ...
He received cultures from Jean Joseph Henri Toussaint, and cultivated them in chicken broth.[70] During this work, a culture of ... who had reported a year earlier that carbolic-acid/heated anthrax serum would immunize against anthrax. These results were ... Wikimedia Commons has media related to Louis Pasteur.. Wikisource has original works written by or about:. Louis Pasteur. ... From Wikipedia, the free encyclopedia. Jump to navigation Jump to search "Pasteur" redirects here. For other uses, see Pasteur ...
... s serum-free product portfolio with the addition of two new cell culture media. ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media Corning Announces Expanded Portfolio of Serum-free Cell ... Corning Announces Expanded Portfolio of Serum-free Cell Culture Media. Corning Announces Expanded Portfolio of Serum-free. ... MSC Medium capitalize on the growing global demand for specifically defined, serum-free media. The media can enable lab ...
Cornings comprehensive line of standard and custom media helps create an optimal environment for all stages of cell culture ... Cell Culture Media Products , Classical, Serum-Free, and Custom Cell Culture Media , Corning. We use cookies to ensure the best ... Serum-free/Specialty Media Solutions Serum-free/Specialty Media Solutions Optimize the cultivation of specific cell types with ... our line of serum-free and reduced-serum media. Optimize the cultivation of specific cell types with our line of serum-free and ...
... more and more scientists are considering serum-free cell culturing. In the webinar titled "Cell culture media: Why go serum- ... Free Lonza webinar to discuss the benefits of working with serum-free cell culture media and provide guidance on how to get the ... Lonza to Host New Webinar - "Cell Culture Media: Why Go Serum-Free?" October 01,2019 *News Release PDF ... How to switch from serum to serum-free culturing with little effort ...
Animal-free research. FCS-free - Culture media without fetal calf serum Animal experiments are increasingly being replaced by ... Furthermore, the produced hPL could meet a large percentage of the global demand for animal serum-free culture media. Moreover ... However, there are FCS-free culture media, for example media containing human platelet lysate (hPL). It is produced from human ... The switch to FCS-free culture media is urgent and imperative in order to no longer cause the suffering of millions of calf ...
... negative effects of culturing cells in serum and you will learn about the advantages of switching to serum-free cell culturing ... Lonza: Cell Culture Media - "Why Go Serum-free?!". In this webinar we discuss the negative effects of culturing cells in serum ... How to switch from serum to serum-free culturing with little effort ... In order to eliminate these unwanted influences, more and more scientists consider serum-free cell culturing. ...
Keratinocyte cultures with serum or serum free media - (May/17/2005 ). can i know suggestions, ideas and opinions about the ... keratinocyte culture??. should it be cultivated under serum contained media or serum free media??. around what are the seeding ... should it be cultivated under serum contained media or serum free media??. around what are the seeding densities do vary with ... the serum free media??. ur suggestions and ideas are very much appreciated! pls! because im confusing using the serum free ...
... or co-cultured in serum or serum-free medium were compared and analyzed via the platform. It was demonstrated that serum ... However, both cell types were successfully co-cultured in 2D using serum-free medium if the initial cell seeding density was ... When mono-cultured on TCP, both HDFs and HaCat cells were less proliferative in medium without serum than with serum. ... were then conducted successfully in serum-free medium. This study demonstrated that the generic research platform had great ...
Tissue Culture > Media, Serum Free products in the SelectScience products and suppliers directory ... MSC NutriStem® XF Medium. Biological Industries. Defined, xeno-free, serum-free culture medium, Designed to support the ... EndoGo™ XF Medium. Biological Industries. Defined, xeno-free, serum-free medium for long-term expansion of large and small ... NutriStem® hPSC XF Medium. Biological Industries. Defined, xeno-free, serum-free medium, Designed for optimal growth and ...
The recipe for the formulation is given, which provides a medium suitable for the proliferation and differentiation of CD34 + ... A serum-free medium which supports the proliferation and differentiation of CD34 + cells purified from normal bone marrow, ... Serum Free 2 951 Medium 1 Serum Free 2 60 Medium 2 IMDM + 20% 2 78 FBSExpt. Serum Free 2 1402 Medium 1 Serum Free 2 36 Medium 2 ... 1 Serum Free 2 132 Medium 1 Serum Free 2 80 Medium 2 IMDM + 20% FBS 2 132Expt. 2 Serum Free 2 90 Medium 1 StemPro 34 2 62 IMDM ...
CellGenix GMP SCGM medium can be used for serum-free cultivation of HSCs as well as expansion and differentiation of NK & CIK ... Stem Cell Growth Medium. CellGenix GMP SCGM is an optimized, xeno-free medium used for the serum-free expansion of low numbers ... Cytokines & Growth Factors Serum-free Media Bioprocessing Containers Supplements & Cells CellGenix® GMP SCGM. ... Efficient serum-free retroviral gene transfer into primitive human hematopoietic progenitor cells by a defined, high-titer, ...
Invitrogens Serum-Free medium is formulated with known concentrations of proteins, growth factors, and other supplements to ... Serum-Free Medium yields gene expression profiles comparable to culture in serum-supplemented mammary epithelial cell culture ... Invitrogen Launches Serum-Free Media Optimized For Human Mammary Epithelial Cell Culture Product News Feb 21, 2007 ... Invitrogen Corporation announces the availability of GIBCO® HuMEC Serum-Free Medium optimized specifically for culture of human ...
... Featured Product Nov 21, 2016 ... Expanding the culture of excellence. NutriStem® hPSC XF Medium is a defined, xeno-free, serum-free medium designed to support ... hPSC XF Medium and the leading competing medium for feeder-free culture. Medium was changed and proliferation was assessed ... The defined, xeno-free formulation of NutriStem® hPSC XF Medium provides consistent media performance and predictable cellular ...
An aqueous extract from toad skin prevents gelatinase activities derived from fetal serum albumin and serum-free culture medium ... Gelatinase activities derived from fetal serum albumin and culture medium of human breast carcinoma cell line MDA-MB-231 were ... Register with J-STAGE for free!. Register Already have an account? Login in here ...
Culturing HSPCs in Serum-Free Media for Cell Therapy Research Whether a protocol uses cell culture methods to expand edited ... serum-free medium undergoes rigorous QC testing to ensure consistent conditions for the culture of HSPCs. StemSpan™ media do ... Media Matters: Serum-Free Hematopoietic Cell Culture for Gene Editing and Gene Therapy Research. STEMCELL™ Technologies ... As compared to other serum-free media, StemSpan™ SFEM II medium supports the superior expansion of CD34+ cells when combined ...
The market research report Serum Free Cell Culture Media Market comprises of market growth, trends, forecasts, key competitor ... Liquid Cell Culture Media. Dry Cell Culture Media. Market by Application. Biopharmaceutical Manufacturing. Tissue Culture & ... Serum-free Cell Culture Media Market Research: Global Status & Forecast by Geography, Type & Application (2015-2025). *SKU ID ... Overview of the Serum-free Cell Culture Media market including production, consumption, status & forecast and market growth. ...
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Optimizing a serum-free/xeno-free culture medium for culturing and promoting the proliferation of human dental pulp stem cells. ... ohio gels kitchen gelsolin gelsons instacart gelsons market Optimizing a serum-free/xeno-free culture medium for culturing ... A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term ... Cell-free culture conditioned medium elicits pancreatic β cell lineage-specific epigenetic reprogramming in mice. dna template ...
Serum Free and chemically defined Cell and Tissue Culture Media Home/Tissue Culture Reagents/Serum Free and chemically defined ... PurMa™ Intestinal Mouse Basal Medium supplied with N1B27 supplement is a chemically defined, serum-free cell culture medium for ... Our proprietary serum free media represent the next generation of chemically defined media to preserve various Primary cells ... PurMa Primary Cell Culture Essential Media (PPEM). This media is specifically designed to preserve and maintain the growth of ...
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... protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal ... This page segues to comprehensive insights on how riboflavin and other important cell culture components affect the performance ... Sigmas Cell Culture Media Expert provides in depth discussion of this and other serum-free and protein-free media supplements ... used as a starting formulation for development of proprietary serum-free, or protein-free cell culture media for Chinese ...
... protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page ... How the trace metal selenium and other cell culture components affect the performance of serum-free, ... Serum-Free/Protein Free Hybridoma Medium; Swims S-77 Medium; Waymouth Medium MB; Williams Medium E and various proprietary ... Our Cell Culture Media Expert provides in depth discussion of this and other serum-free and protein-free media supplements. The ...
found in: HyClone™ HyCell™ TransFx-H Media, Phosphate-Buffered Saline (PBS), HyClone™ Molecular Biology Grade Water, Hepatocyte ... Hepatocyte Culture Media Kit Corning. Corning® Hepatocyte Culture Media Kit, a serum-free, fully-defined medium. (500 ml) ... QBSF®-56 Serum Free Medium Quality Biological. QBSF-56 is a proprietary serum-free formulation engineered to support ... medium in the process of weaning fastidious cells from growth in a serum-supplemented medium to a low protein serum-free medium ...
Serum-free cell culture medium. Introduction. Serum-free mediums allow users to standardize their cell culture conditions. It ... serum-free medium, e.g. 15 ml DMEM (not serum-free) + 5 ml serum-free medium (FreeStyle™ 293 Expression Medium) for a T75 flask ... Testing serum free medium. Serum free medium was obtained from AAV-293 cells are not adapted to serum-free growth conditions so ... We raised the serum-free ratio to 100 % over 7 passages.. 100% serum-free cells grow slower compared to the serum-supplemented ...
Culture Media, Serum Free. Active Comparator: Optisol GS Donor cornea is stored in the Optisol GS media prior to implantation. ... Active Comparator: Life 4°C media for cornea storage Donor cornea is stored in the Life 4°C media prior to implantation. ... Other: Corneal donor storage in Optisol GS media solution Donor tissue is preserved in the Optisol media until ready for ... Life 4°C Versus Optisol in Corneal Storage Media. The safety and scientific validity of this study is the responsibility of the ...
... cultures or as airway organoids using the PneumaCult™ culture system. ... In Vitro Airway Culture Media Why Use Serum- and BPE-Free Cell Culture Medium? The PneumaCult™ Culture System ... Why Use Serum- and BPE-Free Cell Culture Medium?. Traditional formulations for airway epithelial cell cultures typically ... PneumaCult™ culture media system has been formulated to be serum- and BPE-free while maintaining the highest possible ...
Shop a large selection of Liquid Classical Cell Culture Media products and learn more about Gibco Neurobasal Medium 500mL:Cell ... I Supplement and either a serum-free supplement (such as B-27 Supplement or N-2 Supplement), or with serum ... Cell Culture & Analysis Cell Culture & Analysis * Cell Culture Dishes, Plates and Flasks ... Gibco Neurobasal Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal ...
To a basic LIT medium containing liver infusion broth and tryptose, a mixture of RPMI 1640 and Medium 199 was added. This ... Abstract A liquid medium without blood or serum was developed for cultivation of hemoflagellates. ... A New Liquid Medium without Blood and Serum for Culture of Hemoflagellates * Moyses Sadigursky, Claudia I. Brodskyn ... A liquid medium without blood or serum was developed for cultivation of hemoflagellates. To a basic LIT medium containing liver ...
... authenticated cells with their ideal media and transfection reagents to create optimized, easy to use protein expression ... Cell Culture Media. Keep your cells healthy, happy and behaving as expected. ... Serum-Free VERO Cell System Host and Packaging Cell Lines ATCC holdings include an array of host and packaging cell lines of ... ATCC Protein and Virus Production Systems match reliable, authenticated cells with their ideal media and reagents to create ...
Serum free cell culture medium. US5118666. Jun 1, 1990. Jun 2, 1992. The General Hospital Corporation. Insulinotropic hormone. ... Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:RINELLA, JOSEPH VINCENT, JR.;REEL/FRAME:011736/0793 ... making possible a multi-use parenteral formulation that is relatively free of protein aggregation. ...
  • Invitrogen Corporation announces the availability of GIBCO® HuMEC Serum-Free Medium optimized specifically for culture of human mammary epithelial cells. (technologynetworks.com)
  • This serum-free medium is formulated with known concentrations of proteins, growth factors, and other supplements to ensure consistent lot-to-lot performance, making it the best choice for researchers using human mammary epithelial cells to understand the biochemical pathways involved in breast cancer progression. (technologynetworks.com)
  • cDNA microarray studies indicate that culture of human mammary epithelial cells in GIBCO® HuMEC Serum-Free Medium yields gene expression profiles comparable to culture in serum-supplemented mammary epithelial cell culture media, allowing for results representative of normal physiological conditions, but overcoming the drawbacks of lot-to-lot serum variability and high background noise contributed by non-specific proteins contained in serum. (technologynetworks.com)
  • A new serum-free defined medium was developed that supports the growth of normal rat mammary epithelial cells. (naver.com)
  • Bovine Pituitary Extract (BPE) is a broadly used supplement to culture a variety of epithelial and endothelial cells. (thomassci.com)
  • Physiologically relevant in vitro models of the human airway, such as air-liquid interface (ALI) cultures or airway organoids using primary nasal, tracheal, or bronchial epithelial cells, are critical for studying respiratory biology, infection, and disease. (stemcell.com)
  • The PneumaCult™ culture system enables expansion and differentiation of human bronchial epithelial cells (HBECs) and human small airway epithelial cells (HSAECs) at the ALI or for generation of functional airway organoids from HBECs, in a serum- and bovine pituitary extract (BPE)-free culture media. (stemcell.com)
  • Expand and differentiate human airway epithelial cells with this complete medium system for airway cultures. (stemcell.com)
  • Traditional formulations for airway epithelial cell cultures typically contain undefined components such as serum and BPE. (stemcell.com)
  • PneumaCult™ culture media system has been formulated to be serum- and BPE-free while maintaining the highest possible performance of the media for culturing human airway epithelial cells. (stemcell.com)
  • The authors wish to retract their research article entitled 'Serum-free-medium-type mesenchymal stem cell culture supernatant exerts a protective effect on A549 lung epithelial cells in acute lung injury induced by H 2 O 2 ', published in Oncology Reports 40, 3033-3039, 2018. (spandidos-publications.com)
  • Highly malignant +SA mouse mammary epithelial cells were grown in culture and maintained in serum-free media. (nih.gov)
  • Chemically defined, animal component-free, protein-free cryopreservation solution for human ES cells and iPS cells. (selectscience.net)
  • PurMa™ Intestinal Mouse Basal Medium supplied with N1B27 supplement is a chemically defined, serum-free cell culture medium for efficient establish. (purmabiologics.com)
  • Our proprietary serum free media represent the next generation of chemically defined media to preserve various Primary cells including Neural , intestinal , Keratocytes, Hybridoma, Endothelial and retinal cells Culture. (purmabiologics.com)
  • Selenium is chemically reactive in solution and has the ability to participate in oxidation/reduction and free radical reactions. (sigmaaldrich.com)
  • Neuro-PURE TM is a proprietary patent pending chemically defined media specifically formulated for use in the growth of human cells and tissue without the need for serum or other animal products. (jeevanbiosciences.com)
  • There are various types of media, and serums are used in cell culture technique such as protein free media, serum free media, chemically defined media, specialty media, classical media, Stem cell media, and lysogeny broth. (bizpr.us)
  • Preferably, the cell culture medium comprises a semi-solid medium, which is a serum free or chemically defined medium. (freepatentsonline.com)
  • 8. A method according to claim 7, in which the cell culture medium is a chemically defined medium. (freepatentsonline.com)
  • The utilization of chemically defined media has been exhibiting slow growth for both bioprocessors and manufacturers. (transparencymarketresearch.com)
  • However, a shift in demand from serum-based to serum-free, serum-sparing, and chemically-defined media has been observed in this market. (transparencymarketresearch.com)
  • FastGro is a fully chemically defined replacement for foetal bovine serum. (labonline.com.au)
  • As a result, serum free and chemically defined media (CDM) have been developed as a matter of good laboratory practice. (wikipedia.org)
  • The concentration of riboflavin in the popular DMEM/Ham's Nutrient Mixture F-12 (50:50) used as a starting formulation for development of proprietary serum-free, or protein-free cell culture media for Chinese Hamster Ovary (CHO) cell based biomanufacturing of heterologous proteins is 0.59 µM. (sigmaaldrich.com)
  • Serum-free and protein-free cell culture systems, especially those developed for biomanufacturing of heterologous proteins and tissue engineering appear to benefit from the addition of exogenous riboflavin in a concentration range from 0.5 to 1.0 µM. (sigmaaldrich.com)
  • The chemistry and bioavailability of riboflavin in cell culture plays an important role in the stability and utility of media used for biomanufacturing of heterologous proteins and tissue engineering. (sigmaaldrich.com)
  • Support transient transfection, growth, and expression of a variety of recombinant proteins in HEK 293 cells with GE Healthcare HyClone HyCell TransFx-H media, a high-performining, animal-derived component-free (ADCF) cell culture media. (thomassci.com)
  • Support production of adenoviral vectors and recombinant proteins in HEK 293 cultures with GE Healthcare HyClone SFM4HEK293 media, a versatile, protein-free cell culture media containing no animal-derived components (ADCF). (thomassci.com)
  • The objective of this study was to determine whether disc cells could be cultured under serum-free conditions and whether they synthesized bovine pancreatic trypsin inhibitor (BPTI)-like serine proteinase inhibitory proteins (SPIs) previously demonstrated for ovine chondrocytes. (biomedsearch.com)
  • Animal sera contain variety of growth factors, cytokines, and proteins required for in vitro cell culture. (atcc.org)
  • Transplantation of freshly isolated EpCAM+ cells or of hHpSCs expanded in culture into NOD/SCID mice results in mature liver tissue expressing human-specific proteins. (rupress.org)
  • The composition comprises a biodegradable acellular matrix, and passaged autologous fibroblasts substantially free of immunogenic proteins, e.g., culture medium serum-derived proteins, integrated within the matrix. (google.es)
  • Also provided is an injectable composition comprising any type of collagen and passaged autologous fibroblasts substantially free of immunogenic proteins, e.g., culture medium serum-derived proteins, for correcting defects in skin, such as wrinkles or scars, and for augmenting tissue in the subject, particularly facial tissue. (google.es)
  • The expression of differentiation markers and stem cell-associated proteins was different between the media. (arvojournals.org)
  • Culture medium D was superior to the other media with regard to the expression of stem cell-associated proteins, proliferation, and cell migration. (arvojournals.org)
  • 3 , 4 Thus, proteins associated with neural stem cells may be expressed in cultured corneal endothelial cells. (arvojournals.org)
  • Animal components such as fetal bovine serum (FBS), human serum, and serum derived proteins like albumin, transferrin, insulin, and growth factors have been popular ingredients in many biological processes. (invitria.com)
  • InVitria has developed and commercialized several recombinant proteins and cell culture media supplements and continues to develop components, media products, and growth factors leveraging its patented technology. (invitria.com)
  • This culture media is generally supplemented with other proteins and growth factors in order to optimize growth. (wikipedia.org)
  • Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses. (routledge.com)
  • The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and divide. (wikipedia.org)
  • This study, investigates the interplay of bovine serum albumin (BSA) with synthesized chitosan nanoparticles (CSNPs) utilizing regular-state fluorescence and UV-vis absorbance spectroscopy in addition to picosecond time-resolved fluorescence approach. (cellxerum.com)
  • The globular protein, bovine serum albumin (BSA), is a major component of fetal bovine serum. (wikipedia.org)
  • Gelatinase activities derived from fetal serum albumin and culture medium of human breast carcinoma cell line MDA-MB-231 were significantly prevented in the presence of toad skin extract. (go.jp)
  • It binds important macromolecules such as serum albumin in cell culture formulations. (sigmaaldrich.com)
  • Neuro-PURE™ is a proprietary DMEM/F-12 base media that contains no animal-derived components or albumin, and efficiently grows all types of human cells and tissue including cancer cells, stem cells, neuronal cells, foreskin fibroblast, and muscle cells. (jeevanbiosciences.com)
  • Different constituents including albumin, hydrocortisone, insulin, transferrin, triiodotyronine, Epidermal Growth Factor (EGF) and Fibroblast Growth Factor were separately added to Basal Defined Medium (BDM). (uclouvain.be)
  • Among them, BDM supplemented with EGF (1 ng/ml), hydrocortisone (100 nM), insulin (1 mu g/ml), triiodotyronine (2 nM) and linoleic acid complexed to albumin (10 mu g/ml) also named 'synthetic BDM' appeared to be the best serum-free nutritive medium and showed similar results as compared to DMEM supplemented with 10% Fetal Bovine Serum (FBS). (uclouvain.be)
  • A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells. (cellxerum.com)
  • A liquid medium without blood or serum was developed for cultivation of hemoflagellates. (ajtmh.org)
  • With suspension adapted recombinant Chinese hamster ovary (CHO) cell lines 11G-S expressing human pro- urokinase (pro-UK) as the object of study, a serum -free medium for the cultivation of recombinant CHO cells in suspension was formulated by using Plackett-Burman design and response surface methodology . (bvsalud.org)
  • Until some years ago serum or crude tissue extracts were used pre- dominantly or exclusively as media supplements for the cultivation of cells. (springer.com)
  • By flow cytometry with FITC-PNA and PE-anti-Thy-1.1 monoclonal antibody, we distinguished four RMEC subpopulations in cultures in both media: Thy-1.1+ cells, PNA+ cells, cells negative to both reagents and cells positive to both reagents. (naver.com)
  • ATCC Protein and Virus Production Systems match reliable, authenticated cells with their ideal media and reagents to create optimized, easy to use systems for protein expression and virus propagation. (atcc.org)
  • The Global Media Sera And Reagents- Cell Culture Market is growing mainly due to the rising incidence of chronic diseases. (bizpr.us)
  • According to a recent study report published by the Market Research Future, The global media sera and reagents- cell culture market is booming and expected to gain prominence over the forecast period. (bizpr.us)
  • Notably, rising burden of chronic diseases is the key factor driving the media sera and reagents- cell culture market. (bizpr.us)
  • Despite these drivers, lack of awareness, and safety concerns are expected to decline the media sera and reagents- cell culture market growth. (bizpr.us)
  • EMD Millipore, Lonza Biosciences, and Thermo Fisher Scientific, Inc., Hi-media Laboratories, Biowest, Merck KGaA are some of the prominent players at the forefront of competition in the media sera and reagents- cell culture market and are profiled in MRFR Analysis. (bizpr.us)
  • The global media sera and reagents- cell culture market is currently dominated by numerous players. (bizpr.us)
  • GE Healthcare is also one of the leading player in media sera and reagents- cell culture market. (bizpr.us)
  • ATCC high-performance media, sera and reagents are recommended for best results and are uniquely formulated according to cell growth recommendations of original cell line depositors and ATCC cell culture specialists. (atcc.org)
  • These high-performance products are the same reagents used to culture and distribute ATCC cell lines, virtually guaranteeing successful cell culture. (atcc.org)
  • ATCC offers a number of commonly used cell culture buffers, stains and dissociation reagents. (atcc.org)
  • These reagents meet the needs of researchers in the areas of maintaining, visualizing, and processing of the cultured cells. (atcc.org)
  • ATCC offers a variety of dissociation reagents for continuous and primary cell cultures. (atcc.org)
  • According to the latest market study published by Transparency Market Research, the global cell culture media, sera, and reagents market will rise at a CAGR of 7.6% from 2015 to 2023. (transparencymarketresearch.com)
  • This is driving the global cell culture media, sera, and reagents market. (transparencymarketresearch.com)
  • In this report, the cell culture market has been extensively examined in terms of media, sera, and reagents. (transparencymarketresearch.com)
  • In cell culture media, cell culture reagents are chemicals used for synthesizing or detecting another substance, which is under study in the culture. (transparencymarketresearch.com)
  • Cell dissociation reagents, cell freezing media, cell washing solutions, amino acids, extracellular attachment matrices, cell washing solutions, and other related products are the commonly used reagents. (transparencymarketresearch.com)
  • In addition, Biological Industries provides cell culture reagents as well as classical and serum-free media. (businesswire.com)
  • This report analyzes the worldwide markets for Cell and Tissue Culture Supplies in US$ Thousands by the following Product Segments: Media, Sera, and Reagents. (marketpublishers.com)
  • It is worth noting that the basal formulations, DMEM/Ham's Nutrient Mixture F-12 (50:50) and Iscove's Modified Dulbecco's Medium (IMDM) are often supplemented with selenium as part of their modifications into proprietary serum-free or protein-free commercial media useful for recombinant heterologous protein, including monoclonal antibody (Mab), manufacturing. (sigmaaldrich.com)
  • And a serum -free medium, SFM-CHO-S for recombinant CHO cells suspension culture was formulated. (bvsalud.org)
  • The maximum cell density of 11G-S cells in SFM-CHO-S in suspension batch culture reached 4.12 x 10(6) cells /mL with a maximum pro-UK activity at 5614 IU/mL, which was superior to the commercial serum -free medium for recombinant CHO cells . (bvsalud.org)
  • BioLamina's Biosilk is a 3D cell culture biomaterial made from recombinant spider silk protein. (labonline.com.au)
  • These cells are robust in culture and can produce a variety of recombinant glycoproteins at high levels in large scales. (labonline.com.au)
  • The low-protein formulation contains only the most essential components required for maintenance of hES and hiPS cells, providing a simplified medium and maintaining the cells' full differentiation potential. (technologynetworks.com)
  • and Serum-Free/Protein Free Hybridoma Medium. (sigmaaldrich.com)
  • This may explain the range of concentrations of riboflavin used in traditional media, where variables such as serum and protein use and storage of the medium affect riboflavin. (sigmaaldrich.com)
  • and in Serum-Free/Protein Free Hybridoma Medium and various proprietary media. (sigmaaldrich.com)
  • Among them, cells cultivated on surface treated membranes in poly(ethylene terephthalate) exhibited the highest protein content with a significant increase in comparison to tissue culture polystyrene, probably because cells are fed on the two faces instead of one. (uclouvain.be)
  • Synthesis of a Kunitz-type serine proteinase inhibitory protein that shares homology with bovine pancreatic trypsin inhibitor by ovine intervertebral disc cells in serum-free alginate bead culture. (biomedsearch.com)
  • Enriched Serum-free/low protein medium without insulin,trasferrin,hormones,or other growth factors.Formulated for the growth and maintenance of a wide varietyof hybridomas, as well as suspension and adherent cell lines. (mpbio.com)
  • Also, presented are data on specific liver gene expression and liver serum-protein production that again show that the hepatocytes and bile duct cell cultures are similar to liver cells found in a pig's own liver. (usda.gov)
  • Serum protein expression, analyzed by 2D-gel electrophoresis of conditioned medium, was remarkably similar between the two cell culture conditions. (usda.gov)
  • However, quantitative RT-PCR analysis showed that serum protein mRNA levels were significantly elevated in the hepatocytes monolayers in comparison to the bile ductule-containing monolayers. (usda.gov)
  • Serum is the amber fluid rich in protein that is separated from coagulated blood. (golden.com)
  • FBS contains a complex array of protein components that are required by many cells to grow which is why it has been successfully used in cell culture. (wikipedia.org)
  • This medium is proprietary and trademark of PurMabiologics, an advanced version of the current media such as IMDM and DMEM for growth, preservation. (purmabiologics.com)
  • Gibco™ DMEM, Powder, High Glucose, No Sodium Bicarbonate Buffer is used in cell culture processing applications. (fishersci.com)
  • Corning™ Dulbecco's Modification of Eagle's Medium (DMEM) 1X1, Powder is hybridoma tested. (fishersci.com)
  • Support cell culture growth and biomanufacturing processes with powdered GE Healthcare HyClone DMEM/F12 1:1 media, manufactured using ISO 9001 certified processes. (thomassci.com)
  • The AAV-293 cells are used for AAV-2 production and are usually grown in (among other chemicals, such as nutrients, antibiotics, growth factors) serum supplemented DMEM medium. (igem.org)
  • Serum free medium was obtained from AAV-293 cells are not adapted to serum-free growth conditions so we had to accustom them to the new growth conditions step by step, starting with 25% serum-free medium, e.g. 15 ml DMEM (not serum-free) + 5 ml serum-free medium (FreeStyle™ 293 Expression Medium) for a T75 flask. (igem.org)
  • STEMPRO® MSC SFM enables hMSC scale up from 1 million to 1 billion cells using 85% less medium, 92% less surface area, 16% less time, 25% less passaging and less effort (less plates, passaging and no pre-qualification of FBS) versus classical medium (DMEM + 10% MSC-Qualified FBS). (thermofisher.com)
  • Morphology of hMSCs when grown in STEMPRO® MSC SFM or classical medium (DMEM + 10% MSC-Qualified FBS. (thermofisher.com)
  • Optimizing a serum-free/xeno-free culture medium for culturing and promoting the proliferation of human dental pulp stem cells. (cellxerum.com)
  • The scrape loading/dye transfer technique showed that most of colonies that developed in a serum-free medium containing EGF, human transferrin, insulin, and hydrocortisone (basal serum-free medium, BSFM) failed to show cell-cell communication. (naver.com)
  • To examine the relative contributions of signaling from the IR and the IGF-IR in MCF10A mobile proliferation, we measured the proliferative response of MCF10A cells to different concentrations of insulin in our serum-cost-free tradition circumstances, including very low concentrations that do not bind the IGFIR. (adenosine-kinase.com)
  • MCF10A cells cultured with out insulin confirmed no significant improve in mobile quantity during the experiment. (adenosine-kinase.com)
  • Figure 1C demonstrates that the IR and IGF-IR were quickly detectable in MCF10A cells cultured with and devoid of insulin in serum-free media, and elimination of insulin from the medium resulted in decline of IR and IGF-IR tyrosine phosphorylation (Figure 1C). (adenosine-kinase.com)
  • Immunoblot investigation confirmed that IRS1 and IRS2 were being also not tyrosine phosphorylated in insulin-unbiased MCF10A cells reworked by TC1 or FGFR2 cultured in serum-free of charge media without having extra insulin (Figure 1D). (adenosine-kinase.com)
  • We noticed that oncogene-remodeled MCF10A cells obtained independence 19244230 of the mitogenic sign originating from the IGF-IR and contrary to nontransformed cells no more time needed insulin for proliferation in serum-free of charge tradition conditions. (adenosine-kinase.com)
  • To evaluate the total of glucose taken up by insulindependent and insulin-impartial cells, we quantified the sum of glucose in the society media collected at the commence and conclusion of the experiment and expressed the distinction relative to mobile quantity. (adenosine-kinase.com)
  • Measurements ended up taken below problems with or devoid of insulin included to the serum-free society media. (adenosine-kinase.com)
  • Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement. (fishersci.com)
  • Gibco™ Essential 8™ Flex Medium Kit is provided in a convenient two-component kit (500mL basal medium and 10mL supplement). (fishersci.com)
  • The use of FCS to supplement cell culture medium for AAV particle production is problematic because even smallest amounts of animal antigens in the administered drug could lead to a strong immune response in patients. (igem.org)
  • 3. A method according to claim 1, in which the method comprises supplementing a basal medium with a supplement comprising the thymidine family member to produce the cell culture medium. (freepatentsonline.com)
  • The use of sera is as a supplement in cell culture studies. (transparencymarketresearch.com)
  • Aiming to support your cell culture needs, our Media and Media Supplement products provide you with the components necessary for the successful culturing and preservation of several cell types. (peprotech.com)
  • Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. (wikipedia.org)
  • As compared to other serum-free media, StemSpan™ SFEM II medium supports the superior expansion of CD34+ cells when combined with cytokine supplements formulated specifically for this purpose (Figure 1). (regmednet.com)
  • Selenium is an essential metal in cell culture that was not recognized in formulations developed prior to 1976 because sufficient quantities of the metal were present in the supplements, such as serum or in the water used to prepare media. (sigmaaldrich.com)
  • In some instance selenium is now added to media based on the above formulations, it is a component of the supplements ITS, SPIT, SPITE series of supplements (see products below). (sigmaaldrich.com)
  • The two-level Plackett-Burman design was used to evaluate the effect of 10 medium supplements on the growth of the 11G-S cells in suspension culture . (bvsalud.org)
  • Platelet-rich Plasma (PRP) is suggested as xenoprotein-free cell-culture medium replacement for animal-derived supplements. (bioportfolio.com)
  • StemSpan™ Serum-Free Expansion Medium (SFEM) has been developed and tested for the in vitro culture and expansion of human hematopoietic cells, when the appropriate growth factors and supplements are added. (stemcell.com)
  • Clontech offers a range of media and supplements for the derivation, maintenance, and expansion of embryonic stem (ES), induced pluripotent stem (iPS), embryonic germ (EG), and neural stem (NS) cells. (clontech.com)
  • Use the tables below to select the right media and supplements for your cell type and use. (clontech.com)
  • Media supplements and antibiotics from ATCC allow you to customize the growth conditions for your cells. (atcc.org)
  • Moreover, a variety of cells could not be cultivated at all under these conditions and often the composition of the cultures changed within rather short periods of time by overgrowth of initially present subpopulations of those cells which grow well in these supplements, as for example fibroblasts. (springer.com)
  • Nevertheless, using these supplements (or fractions thereof), insight could be gained into some of the influences of serum or tissue extract constituents with re- gard to survival, proliferation and differentiation of cells in cul- ture. (springer.com)
  • The recipe for the formulation is given, which provides a medium suitable for the proliferation and differentiation of CD34 + cells for use in human therapeutic protocols. (google.com)
  • The defined, xeno-free formulation of NutriStem® hPSC XF Medium provides consistent media performance and predictable cellular behavior, as well as increased reproducibility in long-term culture (over 50 passages). (technologynetworks.com)
  • ReproCELL's Primate ES Cell Medium is a serum-free formulation for feeder-dependent ES (embryonic stem) / iPS (induced pluripotent stem) cell culture that was jointly developed by ReproCELL and Kyoto University. (reprocell.com)
  • because i'm confusing using the serum free media, but the cells do not show any proliferation. (protocol-online.org)
  • A serum-free medium which supports the proliferation and differentiation of CD34 + cells purified from normal bone marrow, peripheral blood of patients treated with cytokines, and umbilical cord blood is described. (google.com)
  • The present invention relates to a serum-free medium which can support the growth and proliferation of normal human hematopoietic CD34 + cells purified from sources such as normal human bone marrow, the peripheral blood of patients treated with cytokines (termed mobilized CD34 + cells) or umbilical cord blood. (google.com)
  • Therefore, a need exists for developing a serum-free medium which can support the proliferation and differentiation of CD34 + cells. (google.com)
  • In addition, cells cultured in NutriStem® hPSC XF Medium show superior attachment and proliferation rates, making this medium ideal for high-throughput screening applications. (technologynetworks.com)
  • NutriStem® hPSC XF Medium enables excellent proliferation of undifferentiated hES and hiPS cells. (technologynetworks.com)
  • Proliferation of H1 hES cells cultured in Matrigel-coated 96-well plates in NutriStem® hPSC XF Medium and the leading competing medium for feeder-free culture. (technologynetworks.com)
  • Mobile proliferation induced in co-dealt with cultures was equal to the reaction induced by a supraphysiological concentration of IGF-I (200%), which binds both equally the IR and IGF-IR. (adenosine-kinase.com)
  • These final results reveal that less than typical progress ailments, activation of both the IR and IGF-IR plays a position in supporting cell proliferation under serum-cost-free conditions. (adenosine-kinase.com)
  • To investigate the most appropriate media condition for the proliferation and functional maintenance of human corneal endothelial cells (HCECs). (arvojournals.org)
  • The proliferation and morphology analyses demonstrated that there were significant differences between the media. (arvojournals.org)
  • Medium D resulted in higher proliferation rates compared with the other media, while still maintaining the differentiation potential and surface marker expression profile characteristic of HCECs. (arvojournals.org)
  • Therefore it is useful for many applications to grow AAV-293 cells in serum-free medium. (igem.org)
  • Even though cells grew slower and handling was more difficult due to a missing pH indicator, we successfully cultivated AAV-293 cells in serum-free medium. (igem.org)
  • The Corning stemgro MSC Medium was designed for the maximum expansion of human mesenchymal stem cells (MSCs) derived from bone marrow, cord blood, or adipose tissue. (corning.com)
  • A generic research platform with 2-dimensional (2D) cell culture technology, a 3-dimensional (3D) in vitro tissue model, and a scaled-down cell culture and imaging system in between, was utilized to address the problematic issues associated with the use of serum in skin tissue engineering. (mdpi.com)
  • It was demonstrated that serum depletion had significant influence on the attachment of HaCat cells onto tissue culture plastic (TCP), porous substrates and cellulosic scaffolds, which was further enhanced by the pre-seeded HDFs. (mdpi.com)
  • Tissue culture is one of the most widespread laboratory techniques. (selectscience.net)
  • Primary cultures may be derived from blood or tissue explants and thousands of immortalized cell lines are now available from tissue culture collections. (selectscience.net)
  • This product is tissue culture tested including Stem Cells and is available as 500ml sterile filtered unit. (celprogen.com)
  • Neuro-PURE™ is the only commercially available 100% Serum Free and Xeno Free Tissue Culture Media on the Market. (jeevanbiosciences.com)
  • The media also effectively grows mouse cells and other tissue as well. (jeevanbiosciences.com)
  • Several supports have then been tested including tissue culture polystyrene (as a reference), teflon, polycarbonate or poly(ethylene terephthalate) track-etched membranes. (uclouvain.be)
  • In the present study, porcine embryonic (28-30 days), ventral mesencephalic precursor cells were isolated and propagated as free-floating neural tissue spheres in medium containing epidermal growth factor and fibroblast growth factor 2. (hindawi.com)
  • In order to avoid mechanical and enzymatic dissociation and to preserve an organotypic intercellular microenvironment during propagation of neural precursor cells, culturing of tissue microexplants as neural tissue spheres (NTS) was established in our laboratory [ 19 , 20 ]. (hindawi.com)
  • In contrast to the classical cell-aggregate neurospheres [ 21 , 22 ], cells in NTS cultures are never dissociated, neither at the time of tissue sampling and culture set up nor at cell passaging. (hindawi.com)
  • As cells grow and fill up tissue culture dishes, trypsin is an enzyme used to detach cells from the surface of the culture dish in order to split the culture into more culture dishes. (golden.com)
  • Based on application, the cell culture market is categorized into biopharmaceutical production, stem cell research, diagnostics, drug discovery & development, tissue engineering & regenerative medicine, and other applications. (marketpublishers.com)
  • It became obvious from these experiments that serum or tissue extracts did not only supply cells with nutrients or vitamins (which are now constituents of all basic media), but also with hormones as well as growth-, differentiation-, and attachment-factors. (springer.com)
  • Cell culture media are used in research, clinical development and production of biopharmaceuticals as well as in cell and tissue therapy applications. (businesswire.com)
  • For reference see: Morton, H.C., A survey of Commercially Available Tissue Culture Media. (coriell.org)
  • WPI's FluoroDish tissue culture dishes provide high imaging quality for many applications requiring the use of inverted microscopes, such as high-resolution image analysis, microinjection and electrophysical recording of fluorescent-tagged cells. (labonline.com.au)
  • Normal Colony Morphology.H1 hES cells (panel A) and ACS-1014 hiPS cells (panel B) cultured in NutriStem® hPSC XF Medium on Matrigel-coated plates displaycolony morphologies typical of normal feeder-free hES and hiPS cell cultures, including a uniform colony of tightly compacted cells and distinct colony edges. (technologynetworks.com)
  • Obtaining truly differentiated ALI cultures with optimal morphology and functional readouts can be challenging with primary cells. (stemcell.com)
  • Human MSCs grown in STEMPRO® MSC SFM exhibit a primitive morphology compared to control culture (Figure 1). (thermofisher.com)
  • We cultured HCECs in traditional media (medium A or D) and in stem cell media (medium E or N). The morphology of the cells was evaluated by inverted microscopy. (arvojournals.org)
  • Human dermal fibroblasts (HDFs) and immortalized keratinocytes (HaCat cells) mono- or co-cultured in serum or serum-free medium were compared and analyzed via the platform. (mdpi.com)
  • Liver cells were isolated from 1 wk old pigs or young adult pigs (25 and 63 kg live weight) and were placed in primary culture on feeder-cell layers of mitotically blocked mouse fibroblasts. (usda.gov)
  • The composition comprises a biodegradable acellular matrix, and passaged autologous fibroblasts substantially free of immunogenic. (google.es)
  • CellSera's foetal bovine serum is sterile filtered using Sartorius Stedim Biotech triple 0.1 μm disposable filters. (labonline.com.au)
  • can i know suggestions, ideas and opinions about the keratinocyte culture? (protocol-online.org)
  • Our Primary Human Keratinocyte Cell Cultures are optimized to synthesize keratinocyte cells, the predominant cell type in the epidermis. (thermofisher.com)
  • Normal neonatal human keratinocytes (HEKn) ( C-001-5C ) were grown in EpiLife medium and in a keratinocyte medium from a leading competitor. (thermofisher.com)
  • Moreover, the contained growth factors are superior to those of the fetal calf serum. (aerzte-gegen-tierversuche.de)
  • NutriStem® hPSC XF Medium offers the ability to culture human pluripotent cells without the need for high levels of bFGF and other stimulatory growth factors or cytokines. (technologynetworks.com)
  • StemSpan™ media do not contain cytokines or growth factors, allowing researchers the flexibility to establish cultures that meet their specific requirements. (regmednet.com)
  • In culture, stem cell scientists and engineers seek to program cells in a predictable way by supplying growth factors, physical cues or modifying the expression of genes that control stemness or key cell type specific genes. (golden.com)
  • Serum-free media uses synthetic or purified ingredients to provide nutrients and growth factors that support growth and survival of cells in culture. (golden.com)
  • The cell culture media is a nutrient broth that contains growth factors, vitamins, minerals, salts, energy sources, and amino acids cells need to survive, grow and perform cellular functions. (golden.com)
  • In course of time experiments were performed in which serum enriched with hormones and other growth factors was used to successfully culti- vate those cells which could not survive in serum-supplemented media alone. (springer.com)
  • This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications. (wikipedia.org)
  • Serum is stored frozen to preserve the stability of components such as growth factors. (wikipedia.org)
  • In cell biology, cells are commonly grown in medium supplemented with serum. (lonza.com)
  • The cells were used for AAV production, and we produced similar amounts of virus particles compared to cells grown in FCS-supplemented medium. (igem.org)
  • Cultures grown in EpiLife medium demonstrated population doublings over an extended period compared to cells grown in the competitor's medium. (thermofisher.com)
  • The cells were grown encapsulated in calcium alginate microspheres under serum-free conditions for 10 days. (biomedsearch.com)
  • cultured meat , a product that also aims to be grown feeder-free and serum-free to avoid relying on animal use. (golden.com)
  • induceded pluripotent cells (iPS cells) from cow, pig, chicken, tuna, salmon and lobster that are grown serum free and feeder free. (golden.com)
  • Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their specific cell type. (wikipedia.org)
  • ATCC media is the best way to guarantee robust cell growth especially when reviving cells from cryopreservation. (atcc.org)
  • The company offers complete media solutions including a strong portfolio of clinical-grade media for cultivating cells and stem cells and for cryopreservation, applications that are both particularly relevant in advanced therapy research and clinical development. (businesswire.com)
  • Within sera, newborn calf and adult bovine serum, fetal bovine serum, and others are the components of the market. (transparencymarketresearch.com)
  • cell growth and survival factors to animal serums and particularly fetal bovine serum (FBS) are being developed for a number of reasons, to suit research, therapeutic and food industries. (golden.com)
  • The code consists of an abbreviation of the culture media followed by a number, indicating the percentage of fetal bovine serum, and the letter "I" or "U". "I" indicates that the fetal bovine serum is heat inactivated at 56۳ for 1/2 hour before it is added to the culture medium. (coriell.org)
  • U" indicates that the fetal bovine serum is used without prior inactivation (not inactivated). (coriell.org)
  • medium containing 5% horse serum/fetal bovine serum. (bio.net)
  • Fetal bovine serum (FBS) comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. (wikipedia.org)
  • Fetal bovine serum is a by-product of the dairy industry. (wikipedia.org)
  • Fetal bovine serum, as with the vast majority of animal serum used in cell culture, is produced from blood collected at commercial slaughterhouses from dairy cattle that also supply meat intended for human consumption. (wikipedia.org)
  • The first stage of the production process for fetal bovine serum is the harvesting of blood from the bovine fetus after the fetus is removed from the slaughtered cow. (wikipedia.org)
  • When processed by a reputable commercial serum supplier, the sterilized fetal bovine serum is subjected to stringent quality control testing and is supplied with a detailed Certificate of Analysis. (wikipedia.org)
  • We routinely freeze cells in 10%DMSO - 90%FCS, and have NOT been successful in freezing hybridoma cells that have been adapted to serum free conditions. (bio.net)
  • In serum-free medium containing 1% DMSO and 1 µM dexamethasone, confluent monolayers of hepatocytes formed and could be maintained for several weeks. (usda.gov)
  • When isolated liver cells were cultured in medium without dexamethasone but with 0.5% DMSO, monolayers of cholangioctyes formed that subsequently self-organized into networks of multicellular ductal structures, and whose cells had monocilia projecting into the lumen of the duct. (usda.gov)
  • The Corning Hepatocyte Maintenance Medium is optimized to cultivate primary human hepatocytes, a well-established in vitro model system for studying preclinical drug-induced liver injury (DILI). (corning.com)
  • Animal experiments are increasingly being replaced by cell cultures and organs-on-chips, utilizing human cells derived as waste materials after surgeries. (aerzte-gegen-tierversuche.de)
  • For their survival and growth, the human cells need to be immersed in a culture medium, i.e. a solution containing different nutrients. (aerzte-gegen-tierversuche.de)
  • However, there are FCS-free culture media, for example media containing human platelet lysate (hPL). (aerzte-gegen-tierversuche.de)
  • In addition, there is a wide range of other FCS-free culture media that are derived synthetically or from human blood. (aerzte-gegen-tierversuche.de)
  • Defined, xeno-free, serum-free medium, Designed for optimal growth and expansion of human iPS and hES cells. (selectscience.net)
  • Defined, xeno-free, serum-free culture medium, Designed to support the isolation and expansion of human mesenchymal stem cells (MSCs). (selectscience.net)
  • CellGenix GMP SCGM is an optimized, xeno-free medium used for the serum-free expansion of low numbers of isolated human hematopoietic stem and progenitor cells (HSCs/CD34 + cells). (cellgenix.com)
  • NutriStem® hPSC XF Medium is a defined, xeno-free, serum-free medium designed to support the growth and expansion of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells in a feeder-free environment. (technologynetworks.com)
  • H9.2 hES cells were cultured for 11 passages in NutriStem® hPSC XF Medium using a human foreskin fibroblast (HFF) feeder layer. (technologynetworks.com)
  • In order to modify or expand primary human hematopoietic cells, they must first be isolated from the body and then cultured under conditions that allow for their modification. (regmednet.com)
  • This group cultured human CD34+ cells in StemSpan™ medium and cytokines as a pre-stimulation step ahead of electroporation, before xenotransplantation into mice or additional in vitro culture. (regmednet.com)
  • Explore whether or not your airway cultures truly model the human airway. (stemcell.com)
  • Together, these findings provide newly defined culture conditions for improved ex vivo culture of primary human AML cells. (nih.gov)
  • When combined with the appropriate cytokines, SFEM has been used for the culture and expansion of hematopoietic cells isolated from other species, including mouse, non-human primate, and dog. (stemcell.com)
  • Lymphocyte Separation Medium (LSM™) is a legendary tool to separate mononuclear cells from human peripheral blood as well as bone marrow and umbilical cord blood. (mpbio.com)
  • While mouse-derived feeder cells are often used in research, human feeder cells (Xeno-free) or feeder-free culture systems are needed for clinical use to avoid patient exposure to animal pathogens. (golden.com)
  • In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. (springer.com)
  • Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today. (springer.com)
  • Human iPSCs cultured using Primate ES Cell Medium with 5 ng/mL bFGF on MEF cells. (reprocell.com)
  • This stem cell media enabled development of the world's first human induced pluripotent stem cells in 2007 by Prof Shinya Yamanaka and his colleagues. (reprocell.com)
  • This stem cell media has been specifically developed to simplify the process of ES and iPS cell culture for human and other primate cells. (reprocell.com)
  • Thermo Fisher Scientific has developed a medium for the development and expansion of human T lymphocytes (T cells) for cell therapy developers using allogeneic workflows. (labonline.com.au)
  • Used as basal medium for supporting the growth of many different mammalian cells. (fishersci.com)
  • 5. A method according to claim 4, in which the basal medium is a minimal medium. (freepatentsonline.com)
  • 6. A method according to claim 4, in which the basal medium substantially lacks the thymidine family member. (freepatentsonline.com)
  • Store basal medium at -20 °C. Medium is stable for 3 years when stored as directed. (reprocell.com)
  • Basel, Switzerland, 1 October 2019 - On 15 October, Lonza will host a free 60-minute webinar that will address current concerns regarding the use of serum in cell culture media, explore viable alternatives and provide guidance on how to adapt cells to a serum-free environment. (lonza.com)
  • Join this webinar to learn about the concerns regarding the use of serum, and find out about viable alternatives. (edudip.com)
  • The study presents a method for the selective in vitro culture, i.e., "in the petri dish," of pig hepatocytes and bile duct cells, i.e., liver cells. (usda.gov)
  • A serum-free, feeder-cell-dependent, selective culture system for the long-term culture of porcine hepatocytes or cholangiocytes was developed. (usda.gov)
  • PurMabiologics is proud to have manufactured a specialized culture media to preserve and maintain the growth of hybridoma (such as NS1 and CHO cell. (purmabiologics.com)
  • This media maintains stemness in neuronal progenitor cells" says Dr. Jie Xu, Ph.D. a researcher at Georgia State University. (jeevanbiosciences.com)
  • DRG neuronal culture and immunocytochemistry. (jneurosci.org)
  • L- and T-type calcium channels in cultured neuronal cell lines are insensitive to tetanus toxin. (uu.nl)
  • The unique mycoplasma detection kit and Mycoplasma Removal Agent (MRA) can completely manage the mycoplasma contamination in your cell culture. (mpbio.com)
  • Whether you require an antibiotic active against gram-positive bacteria, gram-negative bacteria, yeast, or fungi, MP Biomedicals provides a wide range of high-quality antibiotics to treat cell culture contamination. (mpbio.com)
  • These easy-to-use, effective antibiotics can efficiently keep cell cultures contamination-free from a wide range of common microbiological contaminants. (mpbio.com)
  • One of the major issues in cell culture is mycoplasma infection, altering a variety of cellular characteristics and functionalities and leading to experimental artifacts and cell loss. (mpbio.com)
  • Once Mycoplasma has been detected, the infected cell culture should be treated with Mycoplasma Removal Agent (MRA), the most reliable solution for mycoplasma removal and prevention, as recognized in more than 550 scientific publications. (mpbio.com)
  • The worldwide effects of the COVID ailment 2019 (COVID-19) are now beginning to be felt, and will altogether influence the Serum-Free Freezing Culture Media market in 2020. (scientect.com)
  • According to , the Global Serum-free Cell Culture Media Market is estimated to reach xxx million USD in 2019 and projected to grow at the CAGR of xx% during the 2020-2025. (360marketupdates.com)
  • MarketsandResearch.biz presents a new market report namely Global Serum-Free Freezing Culture Media Market Growth 2020-2025 aims to improve the experience by offering an extensive and explicit analysis of Serum-Free Freezing Culture Media market. (stmaryscollegian.com)
  • Monolayer culture colonies developed within a week and remained viable for months in culture. (naver.com)
  • Lonza to Host New Webinar - "Cell Culture Media: Why Go Serum-Free? (lonza.com)
  • In the webinar titled "Cell culture media: Why go serum-free? (lonza.com)
  • In this webinar we discuss the negative effects of culturing cells in serum and you will learn about the advantages of switching to serum-free cell culturing. (edudip.com)
  • In order to optimize culture conditions of bovine pulmonary endothelial cells (CPAE), we have compared different culture media, supports and extracellular matrices. (uclouvain.be)
  • Serum deprivation or withdrawal induces apoptosis in endothelial cells, resulting in endothelial cell dysfunction that is associated with cardiovascular disease. (nature.com)
  • Embryoid bodies (EBs) were generated from H9.2 hES cells cultured for 16 passages in NutriStem® hPSC XF Medium on Matrigel matrix as an evaluation of pluripotency. (technologynetworks.com)
  • We raised the serum-free ratio to 100 % over 7 passages. (igem.org)
  • 150 population doublings in a serum-free, defined medium and with a doubling time of ∼36 h. (rupress.org)
  • Furthermore, the produced hPL could meet a large percentage of the global demand for animal serum-free culture media. (aerzte-gegen-tierversuche.de)
  • We describe a method of growing an animal cell in a culture medium, in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability of the animal cell is increased as a result of the elevated concentration of the thymidine family member in the cell culture medium. (freepatentsonline.com)
  • 19. A method according to claim 1, in which apoptosis of the animal cell in the cell culture medium is reduced to enhance cell viability. (freepatentsonline.com)
  • However, stringent process controls for cell culture that need to be adhered to, ethical concerns with respect to the use of animal sources, and concerns over the use of transgenic plants and animals are negatively impacting the market's growth. (transparencymarketresearch.com)
  • Moreover, cell culture media sans serum and animal derived components are regarded ideal for studying the role and function of drug components that are present in small molecules. (transparencymarketresearch.com)
  • Cell culture media that does not contain a nutrient and growth factor-rich serum derived from animal blood. (golden.com)
  • However, in the 1990s scientists began searching for ways to remove serum and other animal products due to concerns about product quality, safety, and animal stewardship. (invitria.com)
  • These cells can either be taken from primary cultures (directly from the animal) or from cryopreserved secondary cultures. (wikipedia.org)
  • Increasing funding for cell-based research, growing benefits of the cell culture-based vaccines, and rising demand for monoclonal antibodies are expected to drive the overall growth of the cell culture market. (marketpublishers.com)
  • This market is experiencing significant growth due to the growing awareness about the benefits of cell culture-based vaccines, increasing demand for monoclonal antibodies (mAbs), funding for cell-based research, growing preference for single-use technologies, growing focus on personalized medicine, and the launch of advanced cell culture products. (marketpublishers.com)
  • The high growth of this segment is attributed to the commercial expansion of major pharmaceutical companies, growing regulatory approvals for the production of cell culture-based vaccines, and increasing demand for monoclonal antibodies (mAbs). (marketpublishers.com)