Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.
Inflammation of the MIDDLE EAR including the AUDITORY OSSICLES and the EUSTACHIAN TUBE.
CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.
Techniques used in studying bacteria.
The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
Methods for maintaining or growing CELLS in vitro.
The rate dynamics in chemical or physical systems.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Elements of limited time intervals, contributing to particular results or situations.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.
A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Established cell cultures that have the potential to propagate indefinitely.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Proteins prepared by recombinant DNA technology.
The relationship between the dose of an administered drug and the response of the organism to the drug.
The means of interchanging or transmitting and receiving information. Historically the media were written: books, journals, newspapers, and other publications; in the modern age the media include, in addition, radio, television, computers, and information networks.
The sum of the weight of all the atoms in a molecule.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Proteins found in any species of bacterium.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
Substances used to allow enhanced visualization of tissues.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Morphological and physiological development of EMBRYOS or FETUSES.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
Glycoproteins which have a very high polysaccharide content.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
Life or metabolic reactions occurring in an environment containing oxygen.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Embryonic and fetal development that takes place in an artificial environment in vitro.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Inorganic salts of sulfuric acid.
Inflammation of the middle ear with purulent discharge.
A ubiquitous sodium salt that is commonly used to season food.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
Colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds; used in biochemical assays and in diagnosis as indicators, especially in the form of enzyme substrates. Synonym: chromogens (not to be confused with pigment-synthesizing bacteria also called chromogens).
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Signal molecules that are involved in the control of cell growth and differentiation.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
A dipolar ionic buffer.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Morphological and physiological development of EMBRYOS.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.
A compound originally developed as an anticoagulant, but possessing anticomplement action and lowering the bactericidal action of blood. It is used in vitro to inhibit blood coagulation and as a diagnostic reagent to encourage the growth of pathogens in the blood. It is also used to stabilize colloidal solutions such as milk and gelatin. (From Merck Index, 11th ed)
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.
Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
Substances that reduce the growth or reproduction of BACTERIA.
A pair of highly specialized muscular canals extending from the UTERUS to its corresponding OVARY. They provide the means for OVUM collection, and the site for the final maturation of gametes and FERTILIZATION. The fallopian tube consists of an interstitium, an isthmus, an ampulla, an infundibulum, and fimbriae. Its wall consists of three histologic layers: serous, muscular, and an internal mucosal layer lined with both ciliated and secretory cells.
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
The middle layer of blood vessel walls, composed principally of thin, cylindrical, smooth muscle cells and elastic tissue. It accounts for the bulk of the wall of most arteries. The smooth muscle cells are arranged in circular layers around the vessel, and the thickness of the coat varies with the size of the vessel.
Inorganic salts of phosphoric acid.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Interstitial space between cells, occupied by INTERSTITIAL FLUID as well as amorphous and fibrous substances. For organisms with a CELL WALL, the extracellular space includes everything outside of the CELL MEMBRANE including the PERIPLASM and the cell wall.
ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Transport proteins that carry specific substances in the blood or across cell membranes.
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
The body of a fungus which is made up of HYPHAE.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.
The functional hereditary units of BACTERIA.
The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.
The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Adherence of cells to surfaces or to other cells.
A sulfur-containing essential L-amino acid that is important in many body functions.
Techniques used in microbiology.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
An essential branched-chain amino acid important for hemoglobin formation.
An amorphous form of carbon prepared from the incomplete combustion of animal or vegetable matter, e.g., wood. The activated form of charcoal is used in the treatment of poisoning. (Grant & Hackh's Chemical Dictionary, 5th ed)
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Sorbitan mono-9-octadecanoate poly(oxy-1,2-ethanediyl) derivatives; complex mixtures of polyoxyethylene ethers used as emulsifiers or dispersing agents in pharmaceuticals.
A cell line derived from cultured tumor cells.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.
The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
The fertilized OVUM resulting from the fusion of a male and a female gamete.
Centers for storing various parts of the eye for future use.
Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).
Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH).
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
Physiological processes and properties of the BLOOD.
A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.
Insulated enclosures in which temperature, humidity, and other environmental conditions can be regulated at levels optimal for growth, hatching, reproduction, or metabolic reactions.
The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.
An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.
Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
A genus of gram-negative, mostly facultatively anaerobic bacteria in the family MYCOPLASMATACEAE. The cells are bounded by a PLASMA MEMBRANE and lack a true CELL WALL. Its organisms are pathogens found on the MUCOUS MEMBRANES of humans, ANIMALS, and BIRDS.
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)
Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).
The space and structures directly internal to the TYMPANIC MEMBRANE and external to the inner ear (LABYRINTH). Its major components include the AUDITORY OSSICLES and the EUSTACHIAN TUBE that connects the cavity of middle ear (tympanic cavity) to the upper part of the throat.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
Organic esters of thioglycolic acid (HS-CH2COOH).
A cyclic nucleotide derivative that mimics the action of endogenous CYCLIC AMP and is capable of permeating the cell membrane. It has vasodilator properties and is used as a cardiac stimulant. (From Merck Index, 11th ed)
An anti-inflammatory 9-fluoro-glucocorticoid.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.

Axin prevents Wnt-3a-induced accumulation of beta-catenin. (1/4091)

When Axin, a negative regulator of the Wnt signaling pathway, was expressed in COS cells, it coeluted with glycogen synthase kinase-3beta (GSK-3beta), beta-catenin, and adenomatous polyposis coli protein (APC) in a high molecular weight fraction on gel filtration column chromatography. In this fraction, GSK-3beta, beta-catenin, and APC were co-precipitated with Axin. Although beta-catenin was detected in the high molecular weight fraction in L cells on gel filtration column chromatography, addition of conditioned medium expressing Wnt-3a to the cells increased beta-catenin in the low molecular weight fraction. However, Wnt-3a-dependent accumulation of beta-catenin was greatly inhibited in L cells stably expressing Axin. Axin also suppressed Wnt-3a-dependent activation of Tcf-4 which binds to beta-catenin and acts as a transcription factor. These results suggest that Axin forms a complex with GSK-3beta, beta-catenin, and APC, resulting in the stimulation of the degradation of beta-catenin and that Wnt-3a induces the dissociation of beta-catenin from the Axin complex and accumulates beta-catenin.  (+info)

Molecular cloning and characterization of a novel angiopoietin family protein, angiopoietin-3. (2/4091)

Using homology-based PCR, we have isolated cDNA encoding a novel member (491 amino acids) of the angiopoietin (Ang) family from human adult heart cDNA and have designated it angiopoietin-3 (Ang3). The NH2-terminal and COOH-terminal portions of Ang-3 contain the characteristic coiled-coil domain and fibrinogen-like domain that are conserved in other known Angs. Ang3 has a highly hydrophobic region at the N-terminus (approximately 21 amino acids) that is typical of a signal sequence for protein secretion. Ang3 mRNA is most abundant in adrenal gland, placenta, thyroid gland, heart and small intestine in human adult tissues. Additionally, Ang3 is a secretory protein, but is not a mitogen in endothelial cells.  (+info)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (3/4091)

Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease.  (+info)

Interaction of lipopolysaccharide with human small intestinal lamina propria fibroblasts favors neutrophil migration and peripheral blood mononuclear cell adhesion by the production of proinflammatory mediators and adhesion molecules. (4/4091)

Fibroblasts are important effector cells having a potential role in augmenting the inflammatory responses in various diseases. In infantile diarrhea caused by enteropathogenic Escherichia coli (EPEC), the mechanism of inflammatory reactions at the mucosal site remains unknown. Although the potential involvement of fibroblasts in the pathogenesis of cryptococcus-induced diarrhea in pigs has been suggested, the precise role of lamina propria fibroblasts in the cellular pathogenesis of intestinal infection and inflammation caused by EPEC requires elucidation. Earlier we reported the lipopolysaccharide (LPS)-induced cell proliferation, and collagen synthesis and downregulation of nitric oxide in lamina propria fibroblasts. In this report, we present the profile of cytokines and adhesion molecules in the cultured and characterized human small intestinal lamina propria fibroblasts in relation to neutrophil migration and adhesion in response to lipopolysaccharide (LPS) extracted from EPEC 055:B5. Upon interaction with LPS (1-10 micrograms/ml), lamina propria fibroblasts produced a high level of proinflammatory mediators, interleukin (IL)-1alpha, IL-1beta, IL-6, IL-8, tumor necrosis factor (TNF)-alpha and cell adhesion molecules (CAM) such as intercellular cell adhesion molecule (ICAM), A-CAM, N-CAM and vitronectin in a time-dependent manner. LPS induced cell-associated IL-1alpha and IL-1beta, and IL-6, IL-8 and TNF-alpha as soluble form in the supernatant. Apart from ICAM, vitronectin, A-CAM, and N-CAM proteins were strongly induced in lamina propria fibroblasts by LPS. Adhesion of PBMC to LPS-treated lamina propria fibroblasts was ICAM-dependent. LPS-induced ICAM expression in lamina propria fibroblasts was modulated by whole blood, PBMC and neutrophils. Conditioned medium of LPS-treated lamina propria fibroblasts remarkably enhanced the neutrophil migration. The migration of neutrophils was inhibited by anti-IL-8 antibody. Co-culture of fibroblasts with neutrophils using polycarbonate membrane filters exhibited time-dependent migration of neutrophils. These findings indicate that the coordinate production of proinflammatory cytokines and adhesion molecules in lamina propria fibroblasts which do not classically belong to the immune system can influence the local inflammatory reactions at the intestinal mucosal site during bacterial infections and can influence the immune cell population residing in the lamina propria.  (+info)

Generation and characterization of aggrecanase. A soluble, cartilage-derived aggrecan-degrading activity. (5/4091)

A method was developed for generating soluble, active "aggrecanase" in conditioned media from interleukin-1-stimulated bovine nasal cartilage cultures. Using bovine nasal cartilage conditioned media as a source of the aggrecanase enzyme, an enzymatic assay was established employing purified aggrecan monomers as a substrate and monitoring specific aggrecanase-mediated cleavage products by Western analysis using the monoclonal antibody, BC-3 (which recognizes the new N terminus, ARGS, on fragments produced by cleavage between amino acid residues Glu373 and Ala374). Using this assay we have characterized cartilage aggrecanase with respect to assay kinetics, pH and salt optima, heat sensitivity, and stability upon storage. Aggrecanase activity was inhibited by the metalloprotease inhibitor, EDTA, while a panel of inhibitors of serine, cysteine, and aspartic proteinases had no effect, suggesting that aggrecanase is a metalloproteinase. Sensitivity to known matrix metalloproteinase inhibitors as well as to the endogenous tissue inhibitor of metalloproteinases, TIMP-1, further support the notion that aggrecanase is a metalloproteinase potentially related to the ADAM family or MMP family of proteases previously implicated in the catabolism of the extracellular matrix.  (+info)

Bile duct epithelial cells exposed to alpha-naphthylisothiocyanate produce a factor that causes neutrophil-dependent hepatocellular injury in vitro. (6/4091)

The acute hepatotoxicity induced by alpha-naphthylisothiocyanate (ANIT) in rats is manifested as neutrophil-dependent necrosis of bile duct epithelial cells (BDECs) and hepatic parenchymal cells. This hepatotoxicity mirrors that of drug-induced cholangiolitic hepatitis in humans. Since BDECs are primary targets of ANIT-induced toxicity, we hypothesized that after exposure to ANIT, BDECs produce a factor(s) that causes neutrophil chemotaxis and neutrophil-dependent hepatocellular injury. To test this hypothesis BDECs were isolated from male Sprague Dawley rats and incubated with ANIT (6.25, 12.5, 25, or 50 microM) or vehicle for 24 h. The conditioned medium (CM) was collected and placed in the bottom chamber of a two-chambered chemotaxis system, while isolated neutrophils were placed in the top chamber. Chemotaxis was indicated by neutrophil migration through a membrane to the bottom chamber. CM from BDECs exposed to each concentration of ANIT was chemotactic, whereas CM from vehicle-treated BDECs was not. ANIT alone caused a modest degree of chemotaxis at 50 microM. The conditioned media were added to isolated hepatocytes or to hepatocyte-neutrophil cocultures and incubated for 24 h. Hepatocyte toxicity was indicated by alanine aminotransferase release into the culture medium. CM from vehicle-treated BDECs did not cause hepatocyte killing in either hepatocyte-neutrophil cocultures or hepatocyte cultures. In contrast, the addition of CM from ANIT-treated BDECs (CM-BDEC-A) to hepatocyte-neutrophil cocultures resulted in hepatocyte killing. The same CM was not cytotoxic to hepatocyte cultures devoid of neutrophils. The hepatocyte killing could not be explained by residual ANIT in the CM, which was below the limit of detection (< or = 0.5 microM). The addition of antiproteases afforded protection against neutrophil-dependent hepatocellular injury induced by CM-BDEC-A. These results indicate that ANIT causes BDECs to release a factor(s) that attracts neutrophils and stimulates them to injure hepatocytes in vitro.  (+info)

A vascular bed-specific pathway. (7/4091)

The endothelial nitric oxide synthase (eNOS) gene is induced by a variety of extracellular signals under both in vitro and in vivo conditions. To gain insight into the mechanisms underlying environmental regulation of eNos expression, transgenic mice were generated with the 1,600-bp 5' flanking region of the human eNos promoter coupled to the coding region of the LacZ gene. In multiple independent lines of mice, transgene expression was detected within the endothelium of the brain, heart, skeletal muscle, and aorta. beta-galactosidase activity was consistently absent in the vascular beds of the liver, kidney, and spleen. In stable transfection assays of murine endothelial progenitor cells, the 1,600-bp promoter region was selectively induced by conditioned media from cardiac myocytes, skeletal myocytes, and brain astrocytes. Cardiac myocyte-mediated induction was partly abrogated by neutralizing anti-platelet-derived growth factor (PDGF) antibodies. In addition, promoter activity was upregulated by PDGF-AB. Analysis of promoter deletions revealed that a PDGF response element lies between -744 and -1,600 relative to the start site of transcription, whereas a PDGF-independent cardiac myocyte response element is present within the first 166 bp of the 5' flanking region. Taken together, these results suggest that the eNos gene is regulated in the cardiac endothelium by both a PDGF-dependent and PDGF-independent microvascular bed-specific signaling pathway.  (+info)

Colonization of in vitro-formed cervical human papillomavirus- associated (pre)neoplastic lesions with dendritic cells: role of granulocyte/macrophage colony-stimulating factor. (8/4091)

The purpose of this study was to investigate the ability of CD1a+ Langerhans/dendritic cells (LCs/DCs) to infiltrate human papillomavirus (HPV)-associated (pre)neoplastic lesions of the uterine cervix. Migration of LCs/DCs in the presence of keratinocytes derived from normal cervix and HPV-transformed cell lines was evaluated in Boyden chambers and in organotypic cultures and correlated with granulocyte/macrophage colony-stimulating factor (GM-CSF) production by the cells, as determined by ELISA. Conditioned media of HPV-transformed keratinocytes contained lower amounts of GM-CSF and induced a decreased motile response of LCs/DCs in the Boyden chamber assay compared with those of normal cervical keratinocytes. The migration of LCs/DCs in the presence of conditioned media from normal keratinocytes could be blocked by an anti-GM-CSF antibody, and the migration of LCs/DCs in the presence of conditioned media from HPV-transformed keratinocytes could be increased by supplementing the media with recombinant GM-CSF. GM-CSF was also a potent factor in enhancing the colonization of LCs/DCs into organotypic cultures of HPV-transformed keratinocytes, as the infiltration of LCs/DCs in the in vitro-formed (pre)neoplastic epithelium was minimal under basal conditions and dramatically increased after the addition of GM-CSF to the cultures. These results suggest that GM-CSF could play an important role in the recruitment of LCs/DCs into the HPV-transformed (pre)neoplastic cervical epithelium and be useful as a new immunotherapeutic approach for cervical (pre)cancers.  (+info)

We evaluated the therapeutic potential of mesenchymal stem cell-conditioned medium (CM-MSC) as an alternative to cell therapy in an antigen-induced model of arthritis (AIA). Disease severity and cartilage loss were evaluated by histopathological analysis of arthritic knee joints and immunostaining of aggrecan neoepitopes. Cell proliferation was assessed for activated and naïve CD4+ T cells from healthy mice following culture with CM-MSC or co-culture with MSCs. T cell polarization was analysed in CD4+ T cells isolated from spleens and lymph nodes of arthritic mice treated with CM-MSC or MSCs. CM-MSC treatment significantly reduced knee-joint swelling, histopathological signs of AIA, cartilage loss and suppressed TNFα induction. Proliferation of CD4+ cells from spleens of healthy mice was not affected by CM-MSC but reduced when cells were co-cultured with MSCs. In the presence of CM-MSC or MSCs, increases in IL-10 concentration were observed in culture medium. Finally, CD4+ T cells from ...
Inhibition of amyloid β (Aβ)-induced mitochondrial damage is considered crucial for reducing the pathological damage in Alzheimers disease (AD). We evaluated the effect of neural stem cell-conditioned medium (NSC-CDM) on Aβ25-35-induced damage in SH-SY5Y cells. An in vitro model of AD was established by treating SH-SY5Y cells with 40 µM Aβ25-35 for 24 h. SH-SY5Y cells were divided into control, Aβ25-35 (40 µM), Aβ25-35 (40 µM) + NSC-CDM, and Aβ25-35 (40 µM) + neural stem cell-complete medium (NSC-CPM) groups. Cell viability was detected by CCK-8 assay. Apoptosis, reactive oxygen species (ROS) production, and mitochondrial membrane potential (MMP) were detected by flow cytometry. Malondialdehyde content was detected by ELISA assay. Western blot analysis was used to detect cytochrome c release and apoptosis-related proteins. Transmission electron microscopy was used to observe mitochondrial morphology. Cell viability significantly decreased and apoptosis significantly increased in ...
The addition of cardiac fibroblast-conditioned medium to purified cardiomyocyte cultures was associated with 1) cell hypertrophy, 2) vimentin expression in the presence of MyHC, and 3) markedly reduced spontaneous rhythmic contractions intrinsic to untreated cultured cardiomyocytes. These effects on cardiomyocyte structure and chronotropy in fibroblast-conditioned medium differed both in nature and time frame from the dedifferentiation or fetal gene program previously delineated in cardiomyocytes cultured under standard conditions (9, 11-14). For example, the progressive emergence of α-smooth muscle actin across all culture conditions was consistent with the dedifferentiation paradigm in contrast to vimentin expression specific to the fibroblast-conditioned medium (9, 14). These data suggest that cardiac fibroblasts introduced a soluble factor or factors into the medium that altered cardiomyocyte function and phenotype by either 1) a direct cell membrane or intracellular interaction or 2) ...
Oncotarget | Teresa Bernadette Steinbichler, Veronika Metzler, Christian Pritz, Herbert Riechelmann, Jozsef Dudas
Despite all kinds of efforts exploited to combat lung cancer, this disease remains the most frequent cause of cancer-related deaths in China. It is now generally accepted that LCSLCs were the determining tumorigenic cells responsible for lung cancer initiation, development, metastasis and relapse after chemotherapy. Therefore, targeted eradication of LCSLCs is extremely vital for improving the prognosis or even recovery of lung cancer.. In the present study, we isolated, enriched and obtained the CD133+ SFCs from human lung cancer NCI-H446 cells, by CD133+ magnetic separation and stem cell-conditioned medium suspension culture method. The following results confirmed that this subpopulation of cells exhibited higher in vitro self renewal, invasion and tumorigenicity capacity over parental cells, accompanied by an upregulated expression level of stem cell markers, self-renewal and invasion related transcription factors. Collectively, these SFCs exerted stem-like cell characteristics and were ...
p,Inhibition of amyloid β (Aβ)-induced mitochondrial damage is considered crucial for reducing the pathological damage in Alzheimers disease (AD). We evaluated the effect of neural stem cell-conditioned medium (NSC-CDM) on Aβ,sub,25-35,/sub,-induced damage in SH-SY5Y cells. An ,em,in,/em,,em, vitro,/em, model of AD was established by treating SH-SY5Y cells with 40 μM Aβ,sub,25-35,/sub, for 24 h. SH-SY5Y cells were divided into control, Aβ,sub,25-35,/sub, (40 μM), Aβ,sub,25-35,/sub, (40 μM) + NSC-CDM, and Aβ,sub,25-35,/sub, (40 μM) + neural stem cell-complete medium (NSC-CPM) groups. Cell viability was detected by CCK-8 assay. Apoptosis, reactive oxygen species (ROS) production, and mitochondrial membrane potential (MMP) were detected by flow cytometry. Malondialdehyde (MDA) content was detected by ELISA assay. Western blot analysis was used to detect cytochrome c release and apoptosis-related proteins. Transmission electron microscopy (TEM) was used to observe mitochondrial ...
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TY - JOUR. T1 - Transendothelial permeability of chlorpyrifos in RBE4 monolayers is modulated by astrocyte-conditioned medium. AU - Yang, Jian. AU - Mutkus, Lysette A.. AU - Sumner, Darrell. AU - Stevens, James T.. AU - Eldridge, J. Charles. AU - Strandhoy, Jack W.. AU - Aschner, Michael. PY - 2001/12/16. Y1 - 2001/12/16. N2 - The immortalized rat brain endothelium 4 (RBE4) cell line preserves many features of the in vivo brain endothelium. It has been used as an in vitro model of the blood-brain barrier (BBB). Astrocyte-endothelial cell interactions are crucial for maintenance of BBB characteristics. The present study investigated morphological and permeability properties of the RBE4 cell line. Immunohistochemical studies showed positive staining in RBE4 cells for E-cadherin, a Ca2+-dependent cell-cell adhesion molecule. Western blot immunoassay showed that RBE4 cells consistently express E-cadherin and that its expression significantly increased (P,0.001) in the presence of ...
In a previous study, we isolated human amniotic fluid (AF)-derived mesenchymal stem cells (AF-MSCs) and utilized normoxic conditioned medium (AF-MSC-norCM) which has been shown to accelerate cutaneous wound healing. Because hypoxia enhances the wound healing function of mesenchymal stem cell-conditioned medium (MSC-CM), it is interesting to explore the mechanism responsible for the enhancement of wound healing function. In this work, hypoxia not only increased the proliferation of AF-MSCs but also maintained their constitutive characteristics (surface marker expression and differentiation potentials). Notably, more paracrine factors, VEGF and TGF-β1, were secreted into hypoxic conditioned medium from AF-MSCs (AF-MSC-hypoCM) compared to AF-MSC-norCM. Moreover, AF-MSC-hypoCM enhanced the proliferation and migration of human dermal fibroblasts in vitro, and wound closure in a skin injury model, as compared to AF-MSC-norCM. However, the enhancement of migration of fibroblasts accelerated by AF-MSC-hypoCM
The microenvironment that surrounds tumor cells is characterized by hypoxic conditions and extracellular acidity. These hostile conditions induce crucial changes in cell behavior and can promote the secretion of many soluble factors such as growth factors, cytokines and enzymes. The lysosomal aspartyl-endopeptidase cathepsin D (CD) is a marker of poor prognosis in breast cancer and is associated with a metastatic risk. In this study, the transport of CD was investigated in a model of breast cancer cells line (MCF-7) cultivated under hypoxia and acidification of media. CD secretion was assessed using Western blot analysis and protease activity was measured in conditioned culture media. We demonstrate that cultured MCF-7 cells secrete an active 52 kDa pCD precursor and report that under hypoxia there was an increased amount of pCD secreted. More surprisingly, extracellular acidification (pH 6 and 5.6) induced the secretion of the fully-mature and active (34 kDa + 14 kDa) double chain CD. Our findings
The retinal pigment epithelium (RPE) maintains the choriocapillaris (CC) in the normal eye and is involved in the pathogenesis of choroidal neovascularization in age-related macular degeneration. studies conflicting results have emerged. Whereas some authors were able to demonstrate that RPE cells produce an inhibitor of endothelial proliferation, 5 others found mitogenic activity for endothelium in RPE conditioned culture medium. 6 We hypothesize that a polarized secretion of growth/inhibitory factors may explain these apparently conflicting and observations. Growth factors produced by RPE include platelet-derived growth factor-BB, transforming growth factor-, and vascular endothelial growth factor-A (VEGF). 7 VEGF, a vascular permeability and angiogenesis factor, is a good candidate for a role in maintaining the CC. VEGF offers been proven to improve vascular permeability in muscle tissue and pores and skin partly by inducing a fenestrated endothelial phenotype. 8 Furthermore, it could become ...
BRCA-1 is a nuclear protein involved in DNA repair, transcriptional regulation, and cell cycle control. Its involvement in other cellular processes has been described. Here, we aimed to investigate the role of BRCA-1 in macrophages M(LPS), M(IL-4), and tumor cell-induced differentiation. We used siRNAs to knockdown BRCA-1 in RAW 264.7 macrophages exposed to LPS, IL-4, and C6 glioma cells conditioned medium (CMC6), and evaluated macrophage differentiation markers and functional phagocytic activity as well as DNA damage and cell survival in the presence and absence of BRCA-1. LPS and CMC6, but not by IL-4, increased DNA damage in macrophages, and this effect was more pronounced in BRCA-1-depleted cells, including M(IL-4). BRCA-1 depletion impaired expression of pro-inflammatory cytokines, TNF-α and IL-6, and reduced the phagocytic activity of macrophages in response to LPS. In CMC6-induced differentiation, BRCA-1 knockdown inhibited TNF-α and IL-6 expression which was accompanied by upregulation ...
Hi, does anyone know ho wto prepare conditioned medium (CM) and add to other cells.. A. This is what I understand based on what Ive read:. 1. Grow cells in 10cm dish 2. after 3 days when cells have reached 70-80% confluence, collect medium. 3. centrifuge collected medium at 1,000 g for 10mins to remove cell depris etc and store in 1ml aliquots in -80deg?. AmI misisng any step.. B. When we use the CM, do we have to dilute with regular medium in 30%, 50% ratio or use CM directly?. Thank you. ...
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Epithelial cells of different tissues or species diverge substantially in their culture requirements. Thus, in vivo-like culture of epithelial cells necessitates optimization of the entire culturing process including transport, isolation, medium composition and culture conditions. In the present study we established a new protocol for a differentiated cell culture system of the porcine cervical epithelium, based on easily accessible slaughterhouse material. The morphology and tested functional markers of our culture system are comparable to the native tissue as shown by histology, immunohistochemistry and alcian blue staining. The use of fibroblast-conditioned medium supported proliferation of cervical epithelial monolayers suggesting that stromal growth factors or cytokines released into the medium are required for cell growth in these epithelia. The supplementation of the conditioned medium with EGF further optimized proliferation and mitochondrial activity of the cervical epithelial cells. ...
Cells have an internal clock. This clock tells them how many times they can divide before they must exit the loop by committing cell suicide. This kind of cell death is called apoptosis (pronounced apohtosis).. Apoptosis is a natural cell process which affects many things. For example, when a human fetus is developing, many vestigial (ancient but no longer necessary) features begin to develop, but mysteriously stop dividing at some point during development. This is another form of apoptosis in action. External factors can also induce a cell to undergo apoptosis. One example is found in our immune system. Certain cells produce factors that are designed to induce apoptosis in the foreign cells they encounter. Other apoptosis inducers include glucocorticoids and radiation. There are many others. Cancer cells grow out of control. They do not know when to stop dividing. They are resistant to the normal factors which induce apoptosis in healthy cells. If you have a normal liver cell and a ...
floating THP-1 cells - medium change advice - posted in Tissue and Cell Culture: Hello, I was adamantly asked to culture THP-1 cells and did so, by thawing a cryovial and seeding the cells in a 25 cm flask. 3 days later 99% of cells are floating, which I reckon is OK. Isnt it? So, do I need to change the medium before they reach ~8x 10^5 cells/ml and are ready to be passed? Since these are floating cells, how do I do that? Also, would a 1/3 split ratio be OK for these cells? Th...
A soluble factor produced by HTU-34 cells is responsible for αvβ3 ligand-binding activity and recruitment to FCs. (A) Adhesion assay. Treatment of HTU-34 cel
Dehydrated Culture Media. There are as many differents requirements for as many differents media.Youll find the peptone that fits your requirement here
Ingredients: Water (Aqua), Human Fibroblast Conditioned Media, SD Alcohol 40, Propylene Glycol, Polysorbate 20, Xanthan Gum, Tetrahexyldecyl Ascorbate, Tocopheryl Acetate, Menthyl Lactate, Lactic Acid, Sodium Hyaluronate, Phenoxyethanol, 1, 2-Hexanediol, Caprylyl Glycol.Storage: Store at 4°C-20°CTopical use only. Not intended for injection.Manufactured for AQ Skin Solutions Inc.390 Goddard, Irvine, CA 92618 USAwww.AQSkinSolutions.comMADE IN USA
LAA047Hu81, FITC-Linked Polyclonal Antibody to Hepatocyte Growth Factor (HGF), 肝细胞生长因子(HGF)多克隆抗体(异硫氰酸荧光素标记), HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
ATCC media have been used by ATCC for over 50 years, with formulations that have proven to be reliable for expansion of most cell types.
ATCC media have been used by ATCC for over 50 years, with formulations that have proven to be reliable for expansion of most cell types.
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Background The efficient derivation of mature (Hb9+) motor neurons from embryonic stem cells is a sought-after goal in the understanding, and potential treatment, of motor neuron diseases. Conditions that promote the robust generation of motor neuron progenitors from embryonic stem cells and that promote the survival of differentiated motor neurons ex vivo are likely, therefore, to be critical in future biological/therapeutic/screening approaches. Previous studies have shown that astrocytes have a protective effect on differentiated motor neurons (in vivo and ex vivo), but it remains unclear whether astrocytes also play a beneficial role in the support of motor neuron progenitors. Here we explore the effect of murine astrocyte-conditioned medium on monolayer cultures of mouse embryonic stem cell-derived motor neuron progenitors. Results Our data show that wild-type astrocyte-conditioned medium significantly increases the number of Olig2+/Hb9- progenitors, which subsequently differentiate into ...
Fingerprint Dive into the research topics of Autocrine-paracrine inhibition of growth hormone and prolactin production by GH,sub,3,/sub, cell-conditioned medium. Together they form a unique fingerprint. ...
Browse Sigma-Aldrichs Media for Food Control to find products in All Food Control Media (alphabetical), Control Media for Fish, Media for Cocoa, Chocolate and Sweets, Media for Corn and Corn Products, Media for Fruits and Vegetable, Media for Meat Control, Media for Products with Sugar, Media for Sour Vegetables, Vinegar, Media for Spices
Mind Control media articles: Concise excerpts from highly revealing media articles on mind control suggest major cover-up. Links to originals provided for verification.
Market Scenario: Cell culture medium is customized and designed to support the growth of specific type of cells. Cell culture medium is composed of
Inquire for Vero Cell Media Market by Manufacturers, Countries, Type and Application, Forecast till 2022, with free sample copy of the report.
There are two larger, academic programs of study in Media Studies, which focus on different key aspects of the development and impact of media: Media and Culture and Media and Information.
Biowest is one of the leaders in the collection of animal sera, offering the widest range of sera and media for cell culture available on the market.
Learn more about Cell Freezing Media. We enable science by offering product choice, services, process excellence and our people make it happen.
Many congratulations to all who took part in WACCs fourth Global Media Monitoring Project (GMMP)! On November 10, hundreds of women and men in over 120 coun...
Menyediakan informasi pencarian kerja media secara online di internet. Anda bisa mendapatkan informasi lowongan kerja pns, cpns, atau swasta. Ada lowongan di tahun 2017. Cocok buat karir anda selanjutnya.
Objective: To explore the role and mechanism of mesenchymal stem cell (MSC) in modulating human pulmonary microvascular endothelial cell (HPMECs) permeability via hepatocyte growth factor (HGF). Methods: The study introduced a co-cultured model between HPMECs and human mesenchymal stem cell conditioned media (MSC-CM) collected from MSCs after 24 h hypoxia culture, and meanwhile HGF was neutralized in MSC-CM by anti-HGF antibody respectively, followed by lipopolysaccharide (LPS) stimulation. Finally, the following measurements were performed: the permeability of HPMECs, the protein expression of vascular endothelial cadherin (VE-cadherin), Occludin in HPMECs by Western blot, HPMECs apoptosis by Annexin V-FITC/PI and HPMECs proliferation by 3-(4, 5)-dimethylthiahiazo (-z-y1)-3, 5-di- phenytetrazoliumromide(MTT ...
This should be relatively straight forward. Sort the cells into single wells so all descendants are clones of the sorted cell and let them grow until they are dense enough to be transferred into a bigger plate (probably 48 or 24 well). Continue this process until you can go into big cell culture flasks or plates and make sure that you freeze away a number of aliquots as a backup if something goes wrong. Expand the cells further until you have enough cells to do an analysis.. Keratinocytes need some time to get highly proliferative (around a week or so) and if you dont grow them on a layer of feeder cells, usually also need a special conditioned medium. See the references below (if you have trouble accessing the papers, let me know).. What often also helps is the use of conditioned media. I know this from other cells in the skin which often dont like to grow (at least not as primary cells) unless you use conditioned media. For this, take of the media from the same sort of cells (here it would ...
Cell culture media, sera, and reagents market will rise at a CAGR of 7.6% from 2015 to 2023, Due to this, the markets valuation of US$3.7 bn in 2014 will reach US$7.1 bn by 2023.
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Media file loss must be an annoying thing, but you can create media recovery easily after you read this article. Try to get media files back here.
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Xinhua is the state-run news agency, and it supplies most of the national print and TV media. All content is Party-controlled and censored, though there is a
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TY - JOUR. T1 - Limbal fibroblast conditioned media. T2 - A non-invasive treatment for limbal stem cell deficiency. AU - Amirjamshidi, H.. AU - Milani, B. Y.. AU - Sagha, H. M.. AU - Movahedan, A.. AU - Shafiq, M. A.. AU - Lavker, R. M.. AU - Yue, B. Y.T.. AU - Djalilian, A. R.. PY - 2011. Y1 - 2011. N2 - Purpose: Limbal fibroblasts are known to regulate the maintenance and differentiation of the corneal epithelium including the limbal epithelial stem cells. This study examined the effect of limbal fibroblast conditioned media in a mouse model of limbal stem cell deficiency. ethods: Limbal stem cell deficiency was created in C57/Bl6 mice by performing a limbus to limbus epithelial debridement. The mice were treated topically for 3 weeks with conditioned media derived from human limbal fibroblasts. The control mice were treated with skin fibroblast conditioned media or Dulbeccos serum-free medium. Results: The mice treated with limbal fibroblast conditioned media demonstrated substantial growth ...
Background: Acute respiratory distress syndrome (ARDS) caused by high pathogenic avian influenza (HPAI) H5N1 virus infection has resulted in severe illness and high mortality rates among patients. Patients with ARDS are often characterized by impaired alveolar fluid clearance and alveolar edema. An understanding of the mechanism responsible for human alveolar edema will lead to the development of novel therapeutic treatments for ARDS patients. We hypothesized that the paracrine soluble factors angiopoietin-1 (Ang-1) and keratinocyte growth factor (KGF) can resolve alveolar fluid clearance by up-regulating the expression of major sodium and chloride transporters impaired by HPAI H5N1 virus infection. Materials and Methods: Human alveolar epithelial cells grown on transwell inserts were infected with HPAI H5N1 (A/HK/483/97) and low pathogenic avian influenza (LPAI) H1N1 (A/HK/54/98) viruses at MOI 0.1 or incubated with conditioned culture medium containing Ang-1 and/or KGF. At 24 and 48 h ...
Cancer stem cells (CSCs) are considered as the origin of tumor relapse. Here, we investigated the effects of Fructus Viticis total flavonoids (FVTF) on the characteristics of lung cancer stem-like cells (LCSLCs) derived from human small cell lung cancer NCI-H446 cell line and its potential mechanism. Human small cell lung cancer NCI-H446 cell line was cultured in vitro. The CD133+ cells were sorted from NCI-H446 cell line by magnetic separation. The suspended culture with stem cell-conditioned medium was used to amplify CD133+ sphere-forming cells (SFCs). The stem cell characteristics of CD133+ SFCs were evaluated using cell self-renewal capacity by tumor sphere formation assay, migration and invasion capacity by Transwell assay, tumorigenicity by xenograft model in nude mouse and cancer stem cell markers expression levels by western blot. The effects of FVTF on the properties of LCSLCs were examined by tumorsphere formation assay and transwell chamber assay. The expression level of p-Akt was determined
Decreased albumin synthesis by hepatocytes in liver injury is thought to occur in response to Kupffer cell-derived acute-phase cytokines. In this study we used hepatocytes maintained in a differentiated phenotype, by culture on a laminin-rich gel substratum (Engelbreth-Holm-Swarm matrix), to investigate the effects of Kupffer cell-conditioned medium and purified cytokines (interleukin-1, interleukin-6 and tumor necrosis factor-a) on albumin synthesis. Kupffer cell-conditioned medium caused a reversible decrease in albumin synthesis to 64.7% of control (p , 0.01, Wilcoxons rank sum test, n = 11) on day 2. Repeated doses caused further dose-dependent reversible responses. The same result was obtained when protease inhibitors (α1-antitrypsin and α2-macroglobulin) were added to Kupffer cell-conditioned medium (n = 3), thus eliminating the potential effect of matrix degradation. Pure interleukin-1, interleukin-6 and tumor necrosis factor-α also inhibited albumin synthesis (p , 0.05, Wilcoxons ...
MSSF - Macrophage Stimulated Soluble Factor. Looking for abbreviations of MSSF? It is Macrophage Stimulated Soluble Factor. Macrophage Stimulated Soluble Factor listed as MSSF
Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Cornings comprehensive line of standard and custom media helps create an optimal environment for all stages of cell culture growth and scaling.
In addition to customized and proprietary cell culture media, we also provide classical media for vaccines, mAbs and recombinant proteins as well as stem cell applications.
Phenion cell culture media are developed to serve the delicate nutrition needs for individual cell types and 3-dimensional complex skin tissues
Proximal cells produce and activate more TGF-β than distal cells. (A) Heat-activated conditioned medium from proximal and distal cells (4 × 104 cells/24-well
UltraCULTURETM Serum-free Cell Culture Medium, without L-Glutamine, 500 mL. NOTE: This product is temporary unavailable please contact our scientific support team for alternatives ...
Get an answer for Given that each type of media offers strengths and drawbacks, what factors should you consider in developing a media plan for a specific product? and find homework help for other Business questions at eNotes
Impact Diagnostic manufactures culture media. Impact Diagnostic is your economical source for quality microbiological testing materials and health care products.
Video created by University of Pennsylvania for the course English for Media Literacy. In this unit, we will learn about the importance of including people from various races, cultures, and genders in mainstream media.
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Media Radjawali Indonesia media informasi perlombaan burung berkicau Online Nasional. Daftakan Update terbaru perlombaan burung berkicau.Dapatkan Informasi Terupdate seputar dunia Perlombaan burung Berkicau hanya di
Optimize your cell culture with Corning vessels, surfaces and media to boost yield, reduce variability, and increase your chances for success - right from the start.
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NEW YORK - November 29, 2017 - Tribune Media Company (NYSE: TRCO) (Tribune or the Company) today announced an offering of 7,000,000 shares of Trib.... 29 November, ...
Culture-media conditioned by macrophages can be used. Production in cell culture is usually preferred as the ascites technique ... The selective culture medium is called HAT medium because it contains hypoxanthine, aminopterin and thymidine. This medium is ... Cell culture sample contaminants consist primarily of media components such as growth factors, hormones and transferrins. In ... Depending on the complexity of the media required in cell culture and thus the contaminants, one or the other method (in vivo ...
Three colony morphologies can be observed when the bacteria is cultured on solid media. Younger colonies tend to form a flat ... mirabilis is capable of growing in both aerobic and anaerobic conditions; however it grows best under aerobic conditions. ... Furthermore, when L. mirabilis is inoculated onto selective media, no growth can be observed. When grown in a broth culture, ... It is typically found on the edge of a colony after one day of culture. Between three and nine flagella project out from the ...
Banaji, Shakuntala (October 1, 2011). South Asian Media Cultures: Audiences, Representations, Contexts (Anthem South Asian ... The movie hall on the 4th floor is fully air-conditioned. There is a community center, a residential hotel and 40 shops inside ... South Asian Media Cultures: Audiences, Representations, Contexts (Anthem South Asian Studies). Anthem Press. p. 82. ISBN 978- ...
V6 in synthetic media under shaking culture conditions". Bioresour. Technol. 86 (3): 215-219. doi:10.1016/S0960-8524(02)00176-1 ... Both static and agitated cultures are not feasible for large-scale production as static cultures have a long culture period as ... Cellulose production depends heavily on several factors such as the growth medium, environmental conditions, and the formation ... doi:10.1016/S0922-338X(97)82059-4. Bae, S.; Shoda, M. (2005). "Statistical optimization of culture conditions for bacterial ...
The resulting shapes depend on the growth conditions. In particular, stresses (hardness of the culture medium, lack of ... Play media The science of pattern formation deals with the visible, (statistically) orderly outcomes of self-organization and ...
C. canimorsus requires very specific culture media and conditions; enriched media are necessary. C. canimorsus displays ... C. canimorsus requires the right medium for growth. The bacterium cultures well on blood agar plates (heart infusion agar with ... Common practice for culturing isolates is to keep agar plates for one week; sometimes, cultures of C. canimorsus are not ... C. canimorsus requires large amounts of iron to grow, so these conditions are optimal for the bacillus. Of the cases presented ...
... culture, sports and tourism; Encouraging interaction of mass media and other means of communication; Promoting exchange of ... Creating favorable conditions for trade and investment; Aiming for comprehensive and balanced economic growth, social and ... The projects are grouped under six cooperation processes, which are: economy, culture, education, transport, customs, and ... the International Organization of Turkic Culture (TURKSOY) (administrative capital, Ankara) International Turkic Academy ( ...
Also, he has studied culture/ethnicity, media influences, interpersonal processes, self/identity, self-affirmation theory and ... In a third condition, the soundbites were left in, but the audience reaction was edited out. In the unedited condition, the ... In one condition, there was no manipulation of the tape. In another condition, two brief, amusing "soundbites" by Reagan that ... In the condition in which the soundbites were cut out, the preference for Reagan was completely eliminated, showing how strong ...
Several studies have optimized the growing conditions of Bartonella spp. cultures in these liquid media, with no change in ... Riess, T (2008). "Analysis of a novel insect cell culture medium-based growth medium for Bartonella species". Appl Environ ... Insect-based liquid media supports the growth and co-culturing of at least seven Bartonella species, reduces bacterial ... Bartonella growth rates improve when cultured in an enrichment inoculation step in a liquid insect-based medium such as ...
Enhanced somatic embryo production by conditioned media in cell suspension cultures of Daucus carota. Biotechnol Lett 14:837- ... Certain compounds excreted by plant tissue cultures and found in culture media have been shown necessary to coordinate cell ... After callus formation, culturing on a low auxin or hormone free media will promote somatic embryo growth and root formation. ... Plant growth regulators in the tissue culture medium can be manipulated to induce callus formation and subsequently changed to ...
Serum Free Culture Media. Culture media is an instrumental part of cellular agriculture and generating cultured tissue because ... Culture Conditions. As mammalian cells digest and metabolize glucose, they produce byproducts such as lactic acid which ... These stem cells are then immersed into a culture medium so that they can proliferate. Culture media consist of basal media ... Instead, insect cell medium typically uses a basal medium (such as Eagle's Medium, Grace's Insect Medium or Schneider's ...
O. hominis may be cultured from clinical specimens such as nasopharyngeal swabs on solid media including blood agar, chocolate ... It requires microaerobic conditions and high humidity. Colonies grow slowly in a mixed bacterial sample and may require up to 5 ... Lawrence, K.A. (2019). "Method for culturing Candidatus Ornithobacterium hominis". Journal of Microbial Methods. 159: 157-160. ...
Women of color have had a large influence in hip hop culture that often goes unrecognized in the media. When they are ... The conditions in the Bronx and other low income areas were substandard; issues facing the community included insufficient ... After recognizing these trends in hip hop/rap lyrics, mainstream media accredited hip hop culture with being inherently ... In the early 2000s Latina women became the beauty ideal in hip hop culture. Rappers would travel to Brazil and other places in ...
... and by its growth and color reaction on certain types of culture media. When cultured on an eosin methylene blue (EMB) plate, a ... Coliform procedures are performed in aerobic or anaerobic conditions. Typical genera include: Citrobacter Enterobacter Hafnia ... Also can be cultured on Tryptone Bile X-Glucuronide (TBX) to appear as blue or green colonies after incubation period of 24 ... While coliforms themselves are not normally causes of serious illness, they are easy to culture, and their presence is used to ...
Media and Sport". 22 July 2005. Cite journal requires ,journal= (help) "Letter to the Secretary of State for Culture, Media and ... Cite journal requires ,journal= (help) Ove Arup and Partners (30 June 2004). "Commonwealth Institute Building Condition Summary ... On 22 July 2005 the Secretary of State for Culture, Media and Sport, Tessa Jowell, rejected a proposal to remove the building's ... Application by the Commonwealth Institute to the Secretary of State for Culture, Media and Sport for a Review of the Statutory ...
Tourette's is a misunderstood and stigmatizing condition, often mentioned in the popular media. Tourette syndrome was once ... and treatment of TS in the media and popular culture. Tourette syndrome is an inherited neurological disorder with onset in ... His condition was reported to have been aggravated by drug use. Some viewers expressed concern that the show had exploited ... Fresh Air, NPR, Media Player file Retrieved on 20 February 2006. Aykroyd, Terry Gross interview excerpt. Retrieved on 20 ...
... growth was studied by Nguyen for bamboo shoots cultured with and without sugar and different photoperiods on the agar medium. ... Their survival rate was 20% higher than the shoots grown under photomixotrophic conditions. "The Plant List: A Working List of ... The shoots grown under photoautrophic conditions weighed more and produced more new leaves. ... sugar-free medium) Micropropagation as a New Micropropagation and Transplant Production System. Springer. p. 129. ISBN ...
8 days of culture in a serum-free medium 8 days of culture in a serum-free medium with activin A 4 days of culture on membranes ... Improving the culture conditions may increase the maturation rates and the potential of IVM oocytes. Besides that, in mouse ... The oocytes are then cultured in media containing nutrients important for oocyte survival, such as gonadotrophins, growth ... In IVM, immature oocytes are removed from the antral follicle of a woman and then are matured in vitro in a culture rich in ...
Increasing success rate of crystallisation projects Production of digested protein used in growth media to culture bacteria and ... The half life of a peptide bond under normal conditions can range from 7 years to 350 years, even higher for peptides protected ... Analysis of the stability of folded domain under a wide range of conditions. ... points may be degraded much faster than those enzymes whose activity is largely constant under all physiological conditions. ...
Under anoxic conditions, the bacteria inhabiting algae cultures break down the organic material and produce hydrogen sulfide ... Effects of medium salinity". Biomass. Elsevier Ltd. 17 (1): 65-76. doi:10.1016/0144-4565(88)90071-6. Gilbert V. Levin; John R. ... Algal culture is the culturing of algae in ponds or other resources. Maximum productivity occurs when the "exchange rate" (time ... Individual species cultures are also much needed for research purposes. A common method of obtaining pure cultures is serial ...
In embryo culture, the embryo is excised and placed into a culture medium with proper nutrient in aseptic condition. To obtain ... Rapid production of callus after placing the explants in culture medium Stage II: The callus is transferred to other medium ... In Meristem culture the meristem and a few subtending leaf primordia are placed into a suitable growing media. An elongated ... The protoplast are first cultured in liquid medium at 25 to 28 C with a light intensity of 100 to 500 lux or in dark and after ...
"Screening of Pleurotus cornucopiae varieties and culture media suitable to Baoshan local conditions". Acta Agriculturae ... cornucopiae and reported that it in the Shanghai area an appropriate growth medium is cotton-seed hulls and wood-chips, with 65 ...
Retrieved 2010-10-21 Richard T. Robertson School of Media and Culture. "About". Virginia Commonwealth University. Retrieved ... History and Present Condition of the Newspaper and Periodical Press of the United States. Washington, D.C.: Government Printing ... 31, 1800+ Virginia media List of radio stations in Virginia List of television stations in Virginia Media of cities in Virginia ... Journalists from Virginia Virginia Commonwealth University Robertson School of Media and Culture (est. 1978), in Richmond ...
In the past[when?] when bacteria were no longer growing on culture media it was assumed that they were dead. Now we can ... Insects undergo a type of dormancy to survive these conditions, called diapause. Diapause may be obligatory for these insects ... Under typical conditions, molecular chaperones facilitate changes in shape (conformational change) of macromolecules in ... Biostasis is found in organisms that live in habitats that likely encounter unfavorable living conditions, such as drought, ...
Martin G (December 1981). "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by ... Church is a regular contributor to and has appeared widely in the media, including TED venues, NOVA, Faces of America ... "A Bill of Rights for the Age of Artificial Intelligence". Medium. OneZero. Herper, Matthew (27 April 2009). "Going To Church". ... an ad hoc team of editorial advisors for the tech news and media company, Xconomy. Church has received accolades including ...
Martin G.R. (1981). "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by ... "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells". ... S2CID 4156737.CS1 maint: multiple names: authors list (link) Evans, M.J; Kaufman M. (9 July 1981). "Establishment in culture of ...
Martin, GR (1981). "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by ... Differentiated macrophages can self-renew in tissues and expand long-term in culture. Under certain conditions, macrophages can ... Consistent with the transwell experiments, conditioned medium induces and maintains CRCs, which is accompanied by a concomitant ... The activity of the conditioned medium correlates directly with radiation-induced feeder cell apoptosis. Thus, conditional ...
The extracted inner cell mass was cultured on fibroblasts treated with mitomycin-c in a medium containing serum and conditioned ... Martin G (1981). "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by ... After 4-6 days of this intrauterine culture, the embryos are harvested and grown in in vitro culture until the inner cell mass ... Typically this is done in the lab with media containing serum and leukemia inhibitory factor or serum-free media supplements ...
Martin G (1981). "Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by ... Evans M, Kaufman M (1981). "Establishment in culture of pluripotential cells from mouse embryos". Nature. 292 (5819): 154-6. ...
"Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells". ... the formation of hanging drops is labor-intensive and not easily amenable to scalable cultures. Additionally, the media can not ... rather than to the culture substrate. As hESC undergo apoptosis when cultured as single cells, EB formation often necessitates ... When cultured as single cells in the absence of anti-differentiation factors, ESCs spontaneously aggregate to form EBs. Such ...
As a condition to obtain the legal status of a trade union, employee associations need to prove that their leverage is strong ... "From Development to Empowerment: The Self-Employed Women's Association in India". International Journal of Politics, Culture, ... Wikimedia Commons has media related to Trade union.. Wikiquote has quotations related to: Trade union. ... The most common purpose of these associations or unions is "maintaining or improving the conditions of their employment".[2] ...
The issue of healthy eating has long been an important concern to individuals and cultures. Among other practices, fasting, ... Physiologically, eating is generally triggered by hunger, but there are numerous physical and psychological conditions that can ... Media related to Eating at Wikimedia Commons. *Ingestion at Encyclopædia Britannica. *Health-EU Portal - Nutrition ... Eating positions vary according to the different regions of the world, as culture influences the way people eat their meals. ...
Most Americans used CAM to treat and/or prevent musculoskeletal conditions or other conditions associated with chronic or ... Traditional medicine refers to the pre-scientific practices of a certain culture, in contrast to what is typically practiced in ... This fact is often overlooked by media or intentionally kept hidden, with alternative practice being portrayed positively when ... This may be due to a natural recovery from the illness, or a fluctuation in the symptoms of a long-term condition.[119] The ...
Conditions at the Viet Cong camp were basic and food scarce.[121] As Sihanouk's government cracked down on the movement in ... Sâr made little or no attempt to assimilate into French culture[46] and was never completely at ease in the French language.[41 ... Media from Wikimedia Commons *. Quotations from Wikiquote *. Data from Wikidata *A meeting with Pol Pot. Elizabeth Becker of ... albeit one that had been adapted to Cambodian conditions.[404] According to key Khmer Rouge figure Khieu Samphan, a key concept ...
... his condition worsened by the start of the new year and he was put on a ventilator.[67] He died of pneumonia and heart failure ... lyrical contributions including working class tales and numerous characters drawn from personal influences and Celtic culture. ... Wikimedia Commons has media related to Phil Lynott.. *Phil Lynott discography at Discogs ...
This condition is called pneumonia.[20] It is susceptible to clindamycin.[21] ... Diagnosis is generally made based on clinical suspicion along with a positive culture from a sample from virtually any place in ... These invasive pneumococcal diseases include bronchitis, rhinitis, acute sinusitis, otitis media, conjunctivitis, meningitis, ... Streptococcus pneumoniae, or pneumococcus, is a Gram-positive, alpha-hemolytic (under aerobic conditions) or beta-hemolytic ( ...
Ministry of Culture and Tourism. *National Radio and Television Administration. *China Media Group *China Central Television * ... of the CCP as a condition of their continued existence.[4] The Chinese political system allows for the participation of some ...
International Publications Media Group. ISBN 978-1-63267-011-3. .. *. Ebola Virus: New Insights for the Healthcare Professional ... Society and culture. See also: Cultural effects of the Ebola crisis. Weaponisation. Ebolavirus is classified as a biosafety ... His condition worsened and he returned to the hospital on 28 September, where he died on 8 October. Health officials confirmed ... Hewlett, Barry; Hewlett, Bonnie (2007). Ebola, Culture and Politics: The Anthropology of an Emerging Disease. Cengage Learning ...
Culture and economy[edit]. The Indus Valley civilisation was mainly an urban culture sustained by surplus agricultural ... Wikimedia Commons has media related to Harappa.. * * *"Harappa Town Planning"-article by Dr S. ... Condition. Ruined. Ownership. Pakistan. Public access. Yes. Website. www. .harappa. .com. Harappa (Punjabi pronunciation: [ ... Harappan Culture. Retrieved 13 January 2006. *^ [1] Poultry: Identification, Fabrication, Utilization by Thomas Schneller - ...
David Cesarani emphasises the improvised, haphazard nature of Nazi policies in response to changing war time conditions in his ... and that the forthcoming war would be a confrontation between two completely opposing cultures.[32] In May 1941, Gestapo leader ... Twayne Publishers, New York: Ardent Media. p. 35. OCLC 141597.. *^ a b Browning (2004), (2007 ed.: p. 544). ...
Many skin conditions can mimic acne vulgaris, and these are collectively known as acneiform eruptions.[28] Such conditions ... Society and culture[edit]. The costs and social impact of acne are substantial. In the United States, acne vulgaris is ... Springer Science & Business Media. p. 231. ISBN 978-3-662-07677-4. . Archived from the original on 6 September 2017.. ... Genetics is the primary cause of acne in 80% of cases.[2] The role of diet and cigarette smoking in the condition is unclear, ...
Society and culture[edit]. In the visual language of Japanese comics (manga) and animation (anime), a sudden, violent nosebleed ... Wikimedia Commons has media related to Nosebleeds.. *National Library of Medicine - Describes causes, solutions, and prevention ... Continued bleeding may be an indication of more serious underlying conditions.[14] ...
However it was not until the 1880s that Louis Pasteur first isolated and grew it in pure culture. Originally a disease of fowl ... Due to the speed of infection and mortality, birds are in good body condition and do not exhibit the signs of prolonged illness ... *^ Blanchong et al. 2006. Persistence of Pasteurella ... The recognition of this pathological condition is of ever increasing importance for differential diagnosis with avian influenza ...
Conditioning regimens[edit]. Myeloablative[edit]. The chemotherapy or irradiation given immediately prior to a transplant is ... Wikimedia Commons has media related to Hematopoietic stem cell transplantation.. *Bone marrow transplant - How it is performed ... during which neither showed traces of HIV in their blood plasma and purified CD4 T cells using a sensitive culture method (less ... Other conditions[13] treated with stem cell transplants include sickle-cell disease, myelodysplastic syndrome, neuroblastoma, ...
Generation M2: Media in the livesof8-to18-year-olds.HenryJKaiserFamilyFoundation.2010.. *^ Kim JH 2010 ~Kim JH, Lau C, Cheuk K- ... Technoscience and Environmental Justice: Expert Cultures in a Grassroots Movement. MIT Press. pp. 1-18. ISBN 978-0262015790.. ... with disagreements over whether technology improves the human condition or worsens it. Neo-Luddism, anarcho-primitivism, and ... Hughes, Thomas P. (2004). Human-Built World: How to Think About Technology and Culture. Chicago: University of Chicago Press. ...
Comics As a Nexus of Cultures: Essays on the Interplay of Media, Disciplines. McFarland. p. 203. ISBN 978-0-7864-3987-4.. ... The pupil can be adjusted in size and a retinal pigment screens incident light in bright conditions.[22] ... Springer Science & Business Media. ISBN 978-94-017-2470-8.. *. Courage, K. H. (2013). Octopus!, The Most Mysterious Creature in ... Forsythe, J. W.; Hanlon, R. T. (1980). "A closed marine culture system for rearing Octopus joubini and other large-egged ...
Patches don't have gender': What is not open in open source software." New Media & Society 14, no. 4 (2012): 669-683. ... The defendant claimed Artistic License in not adhering to the conditions of the software's use, but the wording of the ... Some research and interviews with members of open-source projects have described a male-dominated culture within open-source ... OpenMRS manages features such as alerting health care workers when patients show warning signs for conditions and records ...
Wikimedia Commons has media related to Category:Centro direzionale (Naples).. The Centro Direzionale (Naples) is a service ... Pritzker Prize, RIBA Gold Medal, AIA Gold Medal, Order of Culture, Praemium Imperiale, Order of Sacred Treasures. ... The external skin of the building makes dual references to both tradition and the modern condition. Tange incorporated vertical ... It was designed for three media companies: a newspaper printing plant, a radio station and a television studio. To allow for ...
Schwann, Arthur Jensen, had demanded a raise and made a media affair of it when it was not granted. As a result, he was written ... She reluctantly accepts, on the condition that she'll hand over her business to Mads in exchange of stock in Skjern's ... Danish Culture Canon. *1970s Danish television series. *1980s Danish television series. *1979 Danish television series debuts ...
... only periodically returning to the brewery to supervise storage conditions or bottling operations.[24][unreliable source?] ... Wikimedia Commons has media related to Sake.. Look up sake in Wiktionary, the free dictionary. ... An Indispensable Guide to Sake and Japanese Culture. *What Does Sake Taste Like? ... Under warm and moist conditions, the kōji-kin spores germinate and release enzymes called amylases that convert the rice ...
Folk medicine consists of the healing practices and ideas of body physiology and health preservation known to some in a culture ... For example, evidence on the effectiveness of acupuncture is "variable and inconsistent" for any condition,[2] but is generally ... Media from Wikimedia Commons *. News from Wikinews *. Quotations from Wikiquote *. Texts from Wikisource ... Emergency medicine is concerned with the diagnosis and treatment of acute or life-threatening conditions, including trauma, ...
Each June, the trustees were expected to keep the Society informed of the general condition of its affairs. The Great Council ... German-American culture in Iowa. *Radical Pietism. *History of Buffalo, New York ... Media from Wikimedia Commons *. News from Wikinews *. Texts from Wikisource *. Travel guide from Wikivoyage ... the Great Council disclosed to the Amana Society that the villages were in dire financial condition. The Depression was ...
... that the properties of the display medium must be accounted for, and that the ambient viewing conditions of the acquisition ... Medium-, and Short-wavelength cone types that are modeled as adapting independently. A 3x3 matrix converts RGB or XYZ to LMS, ... differ from the display viewing conditions. The color balance operations in popular image editing applications usually operate ...
"In Romaniello, Matthew; Starks, Tricia (eds.). Tobacco in Russian History and Culture: The Seventeenth Century to the Present. ... In the medium and long run, a recovery of revenues took place. These results suggest either, that the consumption in bars and ... A study published by the American Society of Heating, Refrigerating and Air-Conditioning Engineers and funded by the Robert ... "In Romaniello, Matthew; Starks, Tricia (eds.). Tobacco in Russian History and Culture: The Seventeenth Century to the Present. ...
Rosenthal, Joel H. Righteous Realists: Political Realism, Responsible Power, and American Culture in the Nuclear Age, (1991). ... The conditions he saw in Germany under the French occupation of the Rhineland dismayed him. They reinforced the pacifist views ... Obama's Theologian, E. J. Dionne and David Brooks debate on Speaking of Faith, American Public Media ... Niebuhr attacked poor conditions created by the assembly lines and erratic employment practices.[31] ...
Mongolian dress has changed little since the days of the empire, because it is supremely well-adapted to the conditions of life ... Wikimedia Commons has media related to Culture of Mongolia.. *Ministry of Education, Culture and Science ... "UNESCO Culture Sector. Retrieved 2011-02-03.. *^ UNESCO has inscribed falconry as a shared intangible heritage element of ... The culture of Mongolia has been heavily influenced by the Mongol nomadic way of life. ...
Pop culture[edit]. The 2008 documentary Under Our Skin courted controversy for promoting 'chronic Lyme disease.'[272] ... He named the lesion erythema migrans.[243] The skin condition now known as borrelial lymphocytoma was first described in 1911.[ ... In Europe, known reservoirs of Borrelia burgdorferi were 9 small mammals, 7 medium-sized mammals and 16 species of birds ( ... Society and culture[edit]. Urbanization and other anthropogenic factors can be implicated in the spread of Lyme disease to ...
In popular cultureEdit. He was caricatured in the Disney cartoons Mickey's Gala Premiere (1933), Mother Goose Goes Hollywood ( ... He gave shows in all weather conditions, many in hospitals, sometimes doing his entire show for a single dying soldier. He ... Wikimedia Commons has media related to Joe E. Brown.. Wikiquote has quotations related to: Joe E. Brown. ...
Psychologist Chris French has noted that "dowsing does not work when it is tested under properly controlled conditions that ... Wikimedia Commons has media related to Dowsing.. *Dowsing at DMOZ. *Footage of water dowser at work ... No researcher has been able to prove under controlled conditions that dowsing has any genuine divining power... A more likely ...
Frog goes extinct, media yawns. The Guardian. 27 October 2016. *^ Mendelson, J.R. & Angulo, A. (2009). "Ecnomiohyla rabborum". ... regarding the Clovis culture. There likely would have been human settlements prior to the Clovis Culture, and the history of ... The most popular theory is that human overhunting of species added to existing stress conditions as the extinction coincides ... Arktos Media. ISBN 978-1907166631. .. *. Loarie, Scott R.; Duffy, Philip B.; Hamilton, Healy; Asner, Gregory P.; Field, ...
Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity. ... Conditioned media collected from microvascular endothelial cells in simulated microgravity were used to culture human ... We cultured human microvascular endothelial cells in simulated microgravity using the rotating wall vessel device developed by ... Accordingly, weightlessness conditions during space flight accelerate bone loss and experimental models in real and simulated ...
Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity. ... P. Dowling and M. Clynes, "Conditioned media from cell lines: a complementary model to clinical specimens for the discovery of ... "Changes of cell-vascular complex in zones of adaptive remodeling of the bone tissue under microgravity conditions," Advances in ... and reproduction in any medium, provided the original work is properly cited. ...
conditioned medium - (May/21/2019 ). Hi, does anyone know ho wto prepare conditioned medium (CM) and add to other cells. ... 3. centrifuge collected medium at 1,000 g for 10mins to remove cell depris etc and store in 1ml aliquots in -80deg? ... B. When we use the CM, do we have to dilute with regular medium in 30%, 50% ratio or use CM directly? ... I would recommend filtering the medium through a 0.1 um filter too, just in case there is some contamination associated with ...
Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. G R ... Such embryonic stem cells were isolated from inner cell masses of late blastocysts cultured in medium conditioned by an ... Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells ... Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells ...
Efficient culture condition optimization. To define the optimal culture conditions for your bioprocess, depending on the system ...
Trophoblast Cell Line Conditioned Medium for in Vitro Culture and Antigenic Characterization of Acute Myeloid Leukemia ... Trophoblast Cell Line Conditioned Medium for in Vitro Culture and Antigenic Characterization of Acute Myeloid Leukemia ... Trophoblast Cell Line Conditioned Medium for in Vitro Culture and Antigenic Characterization of Acute Myeloid Leukemia ... Trophoblast Cell Line Conditioned Medium for in Vitro Culture and Antigenic Characterization of Acute Myeloid Leukemia ...
Effect of Culture Conditions on the Survival of Mammalian Cells without Medium Change. MARTIN M. CLYNES ... Effect of Culture Conditions on the Survival of Mammalian Cells without Medium Change ... Effect of Culture Conditions on the Survival of Mammalian Cells without Medium Change ... Effect of Culture Conditions on the Survival of Mammalian Cells without Medium Change ...
2018) Cell-free culture conditioned medium elicits pancreatic β cell lineage-specific epigenetic reprogramming in mice. Oncol ... Home , Cell-free culture conditioned medium elicits pancreatic β cell lineage-specific epigenetic reprogramming in mice ...
The effect of conditioned media on LEC Fas sensitivity was determined by incubating HLE-B3 cells cultured on either TC plastic ... Lens epithelial cells cultured on plastic are protected from Fas mediated apoptosis by conditioned media from lens epithelial ... Lens epithelial cells cultured on plastic are protected from Fas mediated apoptosis by conditioned media from lens epithelial ... cells cultured on plastic are protected from Fas mediated apoptosis by conditioned media from lens epithelial cells cultured on ...
Human monocytes were isolated from PBMCs by Ficoll gradient media. The co-culture of monocytes and Caco-2 conditioned media was ... The present study aimed to evaluate the effect of conditioned media from CRC cells (Caco-2) on immune responses produced by ... Human monocytes were isolated from PBMCs by Ficoll gradient media. The co-culture of monocytes and Caco-2 conditioned media was ... "Conditioned Medium from Cultured Colorectal Cancer Cells Affects Peripheral Blood Mononuclear Cells Inflammatory Phenotype in ...
... study was undertaken to obtain a better understanding of ST and LT toxin production and release under laboratory conditions. ... Media, Culture Conditions and Treatments for Toxin Release. Production of ST and LT toxins by the prototype strain (H10407) was ... Alternatively, these compounds were added directly to the growth medium and cultures maintained under stirring conditions (250 ... Under the same culture conditions, a smaller difference was observed in isolates producing only ST. In fact, according to the ...
Chao, P.Y., Boon, C.H., Liu, H., Wei, S.T., Cao, T. (2007-05). Culture media conditioned by heat-shocked osteoblasts enhances ... Culture media conditioned by heat-shocked osteoblasts enhances the osteogenesis of bone marrow-derived mesenchymal stromal ... Among all experimental groups, the combination of culture medium conditioned by heat shocked hFOB cells together with chemical ... and culture media conditioned by a human foetal osteoblast cell line (hFOB) had any synergistic effect on the osteogenesis of ...
10(6) CFU/cm2) were recovered when the organisms were grown in the low nutrient media. Regardless of the carbon source, ... Influence of culture conditions on biofilm formation by Escherichia coli O157:H7.. Dewanti R1, Wong AC. ... ECM is probably important for the stability of biofilms; however, at 10 degrees C and under anaerobic conditions, ECM seems to ... and a minimal salts medium (MSM) supplemented with 0.04% of one of the following carbon sources: glucose, glycerol, lactose, ...
Volatile organic compounds from Penicillium viridicatum and Methylobacterium mesophilicum growing on laboratory media and on ... and chlorophenol with growth on laboratory media. In comparison with laboratory media, fewer volatiles were detected from ... Culture Media * Disulfides / analysis * Esters / analysis * Mass Spectrometry * Methylobacterium / metabolism* * Organic ... Volatile organic compounds associated with microbial growth in automobile air conditioning systems Curr Microbiol. 2000 Sep;41( ...
Although direct promotion of germ cell maturation by the cell culture medium could not be observed, morphological evaluation in ... Although direct promotion of germ cell maturation by the cell culture medium could not be observed, morphological evaluation in ... the present study was designed to test the influence of different culture media on germ cell differentiation, Leydig cell ... the present study was designed to test the influence of different culture media on germ cell differentiation, Leydig cell ...
... the culture with WT DG-conditioned media (WT DG Cond) yielded comparable amount of neurospheres to that of complete medium, but ... S5, neither the heat-treated WT DG-conditioned media (WT DG Heat) nor conditioned media derived from non-neurogenic WT cortex ( ... For co-culture experiments, 2 h after the astrocyte culture medium in these wells was replaced by Neural Stem Cell Basal Medium ... Tissue-conditioned Media Culture Assay. This assay was done as described previously with minor modifications24. DG of 2 month- ...
"Culture Media, Conditioned" by people in this website by year, and whether "Culture Media, Conditioned" was a major or minor ... Culture Media, Conditioned*Culture Media, Conditioned. *Conditioned Medium. *Medium, Conditioned. *Culture Medium, Conditioned ... "Culture Media, Conditioned" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... Below are the most recent publications written about "Culture Media, Conditioned" by people in Profiles. ...
Culture Media Grant support * R01 EY005477/EY/NEI NIH HHS/United States ... Direct reprogramming of adult human fibroblasts to functional neurons under defined conditions Cell Stem Cell. 2011 Aug 5;9(2): ... under precisely defined conditions. These human induced neurons (hiNs) exhibit typical neuronal morphology and marker gene ...
Aesthetics and Affect in Ubiquitous Media (The Prosaic Image) by Grace Helen (ISBN: 978-0-415-83063-8); Published by ... This book argues that ubiquitous media and user-created content establish a new perception of the world that can be called ... This book argues that ubiquitous media and user-created content establish a new perception of the world that can be called ... developing a model for making sense of the huge proliferation of images in contemporary culture, discovering rhythms and tempo ...
Strains, media, and culture conditions. A complete list of the bacterial strains used in this study is shown in Supplementary ... During the first hours after being spotted onto the culture medium, wild-type 3610 cells started growing with a spatial ... and cultures adjusted to a cell density of 108 cfu ml−1. In the case of Bacillus and Pseudomonas co-inoculation, cultures were ... a medium optimum for the growth and production of secondary metabolites of Pseudomonas strains; Msgg a medium optimum for the ...
Culture medium conditioned over C6 glioma cells (GCM) contains factors which induce neurite outgrowth from clonal rat ... Culture medium conditioned over C6 glioma cells (GCM) contains factors which induce neurite outgrowth from clonal rat ... Culture Media / analysis. Cytochalasin B / pharmacology. Glioma / metabolism*. Hot Temperature. Nerve Growth Factors / ... 0/Carbohydrates; 0/Culture Media; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 14930-96-2/Cytochalasin B; 64-86-8/ ...
Media and Culture Conditions.. DM0 is identical to DM25 except that it contains no glucose. MC plates have the same formulation ... five 10-ml DM0 cultures were inoculated with 100 μl of 100-fold dilutions of each LB culture. The DM0 cultures were incubated ... These contaminated cultures reach much higher turbidity owing to the high concentration of citrate in the medium, which allows ... Owing to the low concentration of glucose in DM25 medium, the cultures are only slightly turbid when transferred. Occasional ...
This contributes to the formation of a reflection of the culture in media. Conley states that "culture is a projection of ... Social conditioning is directly related to the particular culture that one is involved in. In You May Ask Yourself, Dalton ... Such repetition contributes to basic social conditioning. Ivan Pavlov demonstrated this theory with his infamous conditioned ... Social conditioning bases its principals on the natural need for an animal to be a part of a pack. Sigmund Freud, known as the ...
Cell Culture Media and Supplements * Cell Dyes and Detection Assay Kits * Cell Isolation Products ... Agar Leukocyte Conditioned Medium (Agar-LCM) is a source of colony-stimulating factors for assays of hematopoietic progenitor ... Agar Leukocyte Conditioned Medium Source of colony-stimulating factors for assays of human hematopoietic progenitor cells ... Agar Leukocyte Conditioned Medium. • Fetal bovine serum (10%). • Iscoves MDM. • 1 x 10^4 2-Mercaptoethanol (ME) ...
The organism did not grow in the culture media adjusted to pH 4.0, 5.0, 6.0 and 10.0 (Fig.4). In those media where bacterial ... Culture medium. Agar plate A consisted of 2% Bacto-tryptone, 1% Bacto-yeast extract, 1% NaCl and 2% agar at pH 7.0. This was ... The present study deals with the isolation and identification of a bacterium and describes the effects of culture conditions on ... Initially, the organism was grown in the liquid medium (Fig.1a) and then, in the liquid medium supplemented with calcium (10 mM ...
... and maintained in primary culture in a growth medium based upon Leibovitzs L‐15. Experimental growth medium (VCM) conditioned ... and maintained in primary culture in a growth medium based upon Leibovitzs L‐15. Experimental growth medium (VCM) conditioned ... and maintained in primary culture in a growth medium based upon Leibovitzs L‐15. Experimental growth medium (VCM) conditioned ... and maintained in primary culture in a growth medium based upon Leibovitzs L‐15. Experimental growth medium (VCM) conditioned ...
Media & Culture. Marshala Perkins Marijuana Arrest Mugshot Goes Viral. Marshala Perkins of Dallas, Texas, said she received ... Aretha Franklin Still In Serious Condition. Gina Loudon Faces Backlash For Slavery Comments. ...
Media and Culture Conditions. MOPS minimal medium was used for solid growth test and small scale culture. For solid growth test ... For large scale culture, fermentations were carried out in a 5 L laboratory fermenter. M9 minimal medium (3 L), containing 10 g ... Each of cxylB expressing strain was cultured in MOPS minimal medium containing 4 g/L D-glucose and 10 g/L D-xylose. ... Inoculants were prepared by culturing strains in 150 mL LB medium at 37° C. agitated at 180 rpm for 12 hours, and then ...
Watanabe Y, Matsumoto N, Dezawa M, Itokazu Y, Yoshihara T, Ide C. Conditioned medium of the primary culture of rat choroid ... Watanabe, Y, Matsumoto, N, Dezawa, M, Itokazu, Y, Yoshihara, T & Ide, C 2005, Conditioned medium of the primary culture of rat ... T1 - Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite ... title = "Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite ...
  • We cultured human microvascular endothelial cells in simulated microgravity using the rotating wall vessel device developed by NASA. (
  • Conditioned media collected from microvascular endothelial cells in simulated microgravity were used to culture human osteoblasts and were shown to retard osteoblast proliferation and inhibit their activity. (
  • Hi, does anyone know ho wto prepare conditioned medium (CM) and add to other cells. (
  • I would recommend filtering the medium through a 0.1 um filter too, just in case there is some contamination associated with the feeder cells. (
  • The pluripotency of these embryonic stem cells was demonstrated conclusively by the observation that subclonal cultures, derived from isolated single cells, can differentiate into a wide variety of cell types. (
  • Such embryonic stem cells were isolated from inner cell masses of late blastocysts cultured in medium conditioned by an established teratocarcinoma stem cell line. (
  • This suggests that such conditioned medium might contain a growth factor that stimulates the proliferation or inhibits the differentiation of normal pluripotent embryonic cells, or both. (
  • Extension of the culture for antigenic characterization of acute myeloid leukemia clonogenic cells to more patients than in a previous study confirmed the existence of a subgroup (39%) of patients whose acute myeloid leukemia clonogenic cells constantly expressed late myeloid differentiation antigens recognized by the monoclonal antibody S4-7. (
  • HLE-B3 cells were cultured on 1.0µm pore transparent tissue culture inserts (Falcon) and either treated with soluble anti-Fas IgM (Upstate), control IgM (500ng/ml), test (PEG-FasL) or control (PEG) surfaces gently lowered onto the apical cell surface. (
  • After 48 hours culture the number of living cells remaining was estimated by Hoechst staining and cell counting. (
  • The effect of conditioned media on LEC Fas sensitivity was determined by incubating HLE-B3 cells cultured on either TC plastic or collagen IV with either anti-Fas or control IgM in the presence of conditioned medium from HLE-B3 cells cultured on either TC plastic or collagen IV. (
  • HLE-B3 cells cultured on TC plastic which are normally Fas sensitive (55% apoptotic) became protected from Fas-dependent apoptosis in the presence of conditioned medium obtained from HLE-B3 cells cultured on collagen IV (26% apoptotic). (
  • Conditioned Medium from Cultured Colorectal Cancer Cells Affects Peripheral Blood Mononuclear Cells Inflammatory Phenotype in Vitro', Iranian Journal of Medical Sciences , 44(4), pp. 334-341. (
  • The present study aimed to evaluate the effect of conditioned media from CRC cells (Caco-2) on immune responses produced by PBMCs. (
  • The results showed conclusively that conditioned media promoted osteogenesis of MSC, which was further enhanced by prior heat shock-treatment of the hFOB cells, as well as by the presence of chemical stimuli. (
  • Among all experimental groups, the combination of culture medium conditioned by heat shocked hFOB cells together with chemical stimuli, exhibited the highest level of calcium mineralization, as assessed by Von Kossa staining. (
  • It was found that biofilms developed faster and a higher number of adherent cells (ca. 10(6) CFU/cm2) were recovered when the organisms were grown in the low nutrient media. (
  • Therefore, utilizing an established three-dimensional cell culture system that promotes differentiation of pre-meiotic murine male germ cells as far as elongated spermatids, the present study was designed to test the influence of different culture media on germ cell differentiation, Leydig cell functionality, and overall cell survival. (
  • Single-cell suspensions prepared from 7-day-old rat testes and containing all the different types of testicular cells were cultured for as long as 31 days, with or without stimulation by gonadotropins. (
  • In conclusion, the observations reported here show that in three-dimensional cultures, containing all types of rat testicular cells, the nature of the medium per se exerts a direct influence on the functionality of the rat Leydig cells, but not on germ cell differentiation, due to the lack of proper organization of the Sertoli cells. (
  • In three-dimensional cultures of murine Sertoli, Leydig, peritubular, and germ cells stimulated with gonadotropins, pre-meiotic germ cells differentiate into postmeiotic spermatids, but with very low efficiency ( 6 - 8 ). (
  • In three-dimensional cultures containing all murine testicular cells, testosterone production by the Leydig cells was enhanced in response to stimulation by hCG for as long as 16 days ( 6 ). (
  • It remains to be determined whether similar Leydig cell function can be achieved with testicular cells from other species, including humans, under the same conditions. (
  • Unknown cells containing reflecting platelets and unusual other organelles appeared uniquely in chromatophore cultures of P. dacnicolor in VCM. (
  • Isolation & characterization of angiogenic factors from cultured cells & conditioned media. (
  • HEp 2, HeLa and CHO, a primary culture of mouse mammary adenocarcinoma and from conditioned media of HEp2 and cultured mammary adenocarcinoma cells . (
  • Promotion of cell-substratum adhesion of clonal rat pheochromocytoma cells (PC12) by factors contained in glioma-conditioned medium (GCM): separation of two active factors contained in GCM. (
  • Culture medium conditioned over C6 glioma cells (GCM) contains factors which induce neurite outgrowth from clonal rat pheochromocytoma (PC12) cells. (
  • Addition of GCM promoted the adhesion of PC12 cells specifically to collagen-coated tissue culture dish. (
  • Agar Leukocyte Conditioned Medium (Agar-LCM) is a source of colony-stimulating factors for assays of hematopoietic progenitor cells from light-density fractions of human bone marrow and peripheral blood. (
  • Validating these results, reverse transcription-PCR assays demonstrated that in CWR22-Rv1 cells as well as in LAPC-4 CaP cells, increased putative target gene RNA expression occurs with transient overexpression of mutant β-catenin, treatment of cells with lithium chloride, or with wnt3a-conditioned medium, three distinct modes of experimental wnt/β-catenin pathway activation. (
  • The cells were cultivated in a mineral medium containing soluble starch as sole carbon source. (
  • Liquid medium (approximately 5 ml) was pipetted into the agar plates B and the cells scraped off using a sterile Pasteur pipette. (
  • We report here that fliC insertional mutants constructed in two wild-type EPEC strains were markedly impaired in adherence and microcolony formation on cultured cells. (
  • Flagella purified from EPEC bound to cultured epithelial cells and antiflagella antibodies blocked adherence of several EPEC serotypes. (
  • We determined that eukaryotic cells in culture stimulate expression of flagella by motile and non-motile EPEC. (
  • Isogenic strains mutated in perA (a transcriptional activator), bfpA (a type IV pilin), luxS (a quorum-sensing autoinducer gene) and in the type III secretion genes were reduced for motility in Dulbecco's modified Eagle medium (DMEM) motility agar and produced none or few flagella when associated with epithelial cells. (
  • Growth of these mutants in preconditioned tissue culture medium restored motility and their ability to produce flagella, suggesting the influence of a signal provided by mammalian cells that triggers flagella production. (
  • Recent findings suggested their regenerative action on more mature disc cells, presumably by the secretion of specific factors, w hich has been described as notochordal cell conditioned medium (NCCM). (
  • Methods Porcine NC was kept in 2D monolayer and in 3D alginate bead culture to identify a suitable culture system for these cells. (
  • To test stimulating effects of NC, co-cultures of NC and bovine derived coccygeal IVD cells were conducted in a 1:1 ratio with no direct cell contact between NC and bovine nucleus pulposus cell (NPC) or annulus fibrosus cells (AFC) in 3D alginate beads under normoxia and hypoxia (2%) for 7 and 14 days. (
  • The tube formation of human vein endothelial cells (HUVECs) was determined by using conditioned media of HT-29 cells treated with quercetin co-cultured with HUVECs. (
  • The VEGFR-2 protein expression in HUVECs was determined after they were co-cultured with the quercetin-treated HT-29 cells. (
  • Wu, MC & Yunis, AA 1985, ' Differentiation of cultured leukemia cells induced by endotoxin treated human lung conditioned medium ', Federation Proceedings , vol. 44, no. 5, pp. (
  • To evaluate the effect of conditioned medium from human uterine cervical stem cells (CM-hUCESCs) on corneal epithelial healing in a rat model of dry eye after alkaline corneal epithelial ulcer. (
  • These impediments to the use of DR cells for autografting may be overcome through the development of ex vivo culture systems that support expansion and initial differentiation of primitive progenitors. (
  • Because 2-week culture of DR cells in a stroma 'noncontact' system supplemented with interleukin-3 (IL-3) and macrophage inflammatory protein 1-α (MIP-1α) expands both long-term culture-initiating cells (LTC-ICs) and colony-forming cells (CFCs), we adapted this system to a clinically applicable method for expanding LTC-ICs and CFCs ex vivo. (
  • In initial small-scale studies, DR cells were grown in stroma-conditioned medium (SCM) supplemented with IL-3 with or without additional growth-promoting cytokines and the chemokines PF-4 and BB10010, all approved for clinical use. (
  • An IL-3 dose-dependent expansion of committed progenitors and LTC-ICs was observed when DR cells were cultured in tissue culture plates in SCM+IL-3 for 2 weeks. (
  • Culture of 2-6x10 5 DR cells in gas-permeable bags with SCM+IL-3 resulted in similar CFC and LTC-IC expansion as seen in small-scale cultures. (
  • In addition, we observed that progenitors capable of differentiating to natural killer (NK)-cells were maintained under these conditions. (
  • Finally, we found that BCR/ABL mRNA-negative CFCs and LTC-ICs present in DR - cells selected from steady-state CML marrow could be expanded in large-scale SCM+IL-3 cultures. (
  • We conclude that culture of DR cells for 2 weeks in SCM+IL-3 culture, with or without PF-4 or SCF, results in significant CFC and LTC-IC expansion and lymphoid NK progenitor maintenance. (
  • Human cardiac progenitor cells, cultured as cardiospheres (CSps) or as CSp-derived cells (CDCs), have been shown to be capable of direct cardiac regeneration in vivo. (
  • CDC-conditioned media exerted antiapoptotic effects on neonatal rat ventricular myocytes, and proangiogenic effects on human umbilical vein endothelial cells. (
  • 13,14 CSps are self-assembling spherical clusters that grow in semisuspension culture on poly- d -lysine, and that constitute a niche-like environment: undifferentiated cells proliferate in the core, whereas cardiac-committed cells grow on the periphery in a gradient fashion. (
  • We find that soluble Aβ from Tg2576 conditioned medium (CM) induces CaN activation and subsequent nuclear factor of activated T cells (NFATc4) nuclear translocation, which leads to dendritic spine loss, dendritic simplification, and neuritic dystrophies. (
  • Cells cultured with facial nerve - conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β- tubulin III, NeuN, and NF-200. (
  • On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve - conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). (
  • In contrast to the murine system, long-term hamster bone marrow suspension cultures maintain proliferation of both pluripotent and committed stem cells in the absence of an adherent layer and without addition of exogenous factors, such as hydrocortisone. (
  • Addition of pokeweed-mitogen-stimulated hamster spleen conditioned medium (SCM) to these long-term suspension cultures produces an increase in the number of mixed colonies assayed in soft-agar, These mixed colonies, which contained four cell lineages--granulocytic, erythroid, megakaryocytic, and macrophage--could be generated from cells grown in suspension for over 6 mo. (
  • In contrast, addition of SCM to hamster bone marrow cultures containing both adherent cells and hematopoietic stem cells produced no change in the number of mixed colonies generated in the culture. (
  • In vitro culture of stem cells must be a carefully controlled process, and different stem cell types (such as embryonic, mesenchymal, hematopoietic and neural) each require specific media components, such as supplementation, growth factors and other signaling molecules. (
  • BV-2 cells are cultured and passaged every 3-4 days in fresh complete DMEM. (
  • The Bovine Brain Endothelial Cells Growth Media is very nice since it comes in complete form. (
  • Fungi trigger Streptomyces exploratory growth in part by altering the composition of the growth medium, and Streptomyces explorer cells can communicate this exploratory behaviour to other physically separated streptomycetes using an airborne volatile organic compound (VOC). (
  • Remove medium, add dissociation solution (0.02% EDTA and 5% dialized fetal bovine serum in Ca-Mg free Hanks' BSS) and allow the flask to sit at 37C for 12 minutes or until the cells detach. (
  • Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). (
  • Polyethylene glycol is used to fuse adjacent plasma membranes, but the success rate is low, so a selective medium in which only fused cells can grow is used. (
  • This medium is selective for fused (hybridoma) cells. (
  • Only fused hybrid cells referred to as hybridomas, are able to grow indefinitely in the medium because the spleen cell partner supplies HGPRT and the myeloma partner has traits that make it immortal (similar to a cancer cell). (
  • This can be achieved by the use of a layer of feeder fibrocyte cells or supplement medium such as briclone. (
  • Both GLP-1R agonists accelerated the impaired neurite outgrowth of DRG neurons cultured with Schwann cell-conditioned media that mimicked the diabetic condition. (
  • It is hoped that the data may contribute to the development of a more well-defined and efficient in vitro culture protocol to promote the osteogenesis of MSC for both clinical and non-clinical applications. (
  • Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (
  • The results of this study demonstrate the potential of in vitro culture techniques for bryophyte spore studies and germplasm preservation. (
  • Effects of different medium on morphophysiological traits of Lippia citriodora H.B.K. during in vitro culture condition', Journal of Herbal Drugs (An International Journal on Medicinal Herbs) , 5(3 Special issue (in Persian or Farsi)), pp. 143-150. (
  • After injury, dry eyes treated with CM-hUCESCs significantly improved epithelial regeneration and showed reduced corneal macrophage inflammatory protein-1 alpha (MIP-1α) and TNF-α mRNA expression as compared to untreated eyes and eyes treated with culture medium or sodium hyaluronate ophthalmic drops. (
  • To determine the effect of amniotic membrane-conditioned medium (AMCM), via insulin-like growth factor (IGF)-1 induction, on human corneal epithelial cell (HCEC) proliferation. (
  • HCECs were cultured from corneal limbal tissue with supplemented hormonal epithelial medium (SHEM). (
  • With the use of pure culture of CPECs, we demonstrated that the conditioned medium (CM) from CPECs can enhance neurite outgrowth and survival of cultured neurons derived from rat hippocampus on postnatal day 1 in 24-h cultures. (
  • whereas HLE-B3 LECs are sensitive to Fas ligation when cultured on tissue culture plastic but become Fas resistant when cultured on lens capsule or collagen IV. (
  • Recommended applications/industries: Therefore, Based on the finding, the four medium (MS medium supplemented with BAP (5 mgL -1 )) with the highest rates of shoot fresh weight, leaf number, internodes number, shoot number, root fresh weight, callus diameter and root dry weight can be introduced as a suitable medium of tissue culture in this experimental conditions. (
  • Osteogenic differentiation of MSC was assessed on both day 14 and 21 of ex vivo culture. (
  • Although direct promotion of germ cell maturation by the cell culture medium could not be observed, morphological evaluation in combination with immunohistochemical staining revealed unfavorable organization of tubules formed de novo in the three-dimensional culture, allowing differentiation to the stage of pachytene spermatocytes. (
  • After three months the tumor was removed and placed in culture. (
  • One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. (
  • Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. (
  • The influence of different carbon and nitrogen sources, of ethanol concentration, the optimal pH, temperature and medium composition were evaluated on extracellular polysaccharides (EPS) synthesis and bacterial growth of Gluconacetobacter hansenii LMG1524, and preliminary characterization of EPS was investigated. (
  • Activin β A mRNA was also highly induced in primary cultured murine bone marrow MC (BMMC) after stimulation by IgE receptor cross-linking. (
  • Accordingly, weightlessness conditions during space flight accelerate bone loss and experimental models in real and simulated microgravity show decreased osteoblastic and increased osteoclastic activities. (
  • IOL materials covalently modified with FasL kill some but not all contacting LECs after 48 hours in experimental conditions. (
  • Experimental growth medium (VCM) conditioned by a one‐hour exposure to pieces of ventral skin of adult R. pipiens induced iridophores to assume a broad and stellate appearance, to form confluent sheets, and to proliferate over a nine‐day period. (
  • The book analyses the specific features of these new developments - the components of what can be called a 'general aesthesia' - and it focuses on the originality and innovation of amateur practices, developing a model for making sense of the huge proliferation of images in contemporary culture, discovering rhythms and tempo in this work and showing why it matters. (
  • That the ventral integument of adult frogs (Rana pipiens) contains factor(s) that stimulate iridophore expression (adhesion, morphologic appearance, proliferation) was demonstrated on iridophores derived from tadpoles of R. pipiens and Pachymedusa dacnicolor, and maintained in primary culture in a growth medium based upon Leibovitz's L‐15. (
  • Iridophores in control medium assumed long thin profiles, detached easily, and exhibited no signs of proliferation. (
  • Monitoring cell culture media conditions is highly important for pharma industries in order to improve cell proliferation for applications such as protein synthesis. (
  • The result shows that the NAD(P)H is a key fluorescence indicator of the culture medium condition and cell proliferation. (
  • Furthermore, in three-dimensional collagen gel culture, LRTC formed tubule-like or tubulocystic structures in response to growth factors (hepatocyte growth factor and fibroblast growth factor) that are known to induce kidney cell tubulogenesis in vitro and/or participate in renal regeneration in vivo . (
  • This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve - conditioned medium in the presence or absence of fibroblast growth factor 2 ( FGF-2 ). (
  • The ideal strain would be able to consume all sugars present simultaneously and operate at the optimal temperature conditions needed for cellulase activity. (
  • Growth experiments under anaerobic conditions showed that strain YK-1 was a sulfur-oxidizing obligate chemolithotroph utilizing sulfide, elemental sulfur, thiosulfate, and hydrogen as electron donors and nitrate as an electron acceptor. (
  • The Afmac1 deletion strain of A. fumigatus failed to grow in copper-limited media, and its growth was restored by introducing ctrC . (
  • The preferred alpha-amylases are prepared by culturing a strain of a Bacillus stearothermophilus microorganism in a suitable culture medium. (
  • Optimization of Culture Conditions and Medium Components for Carboxymethyl Cellulase (CMCase) Production by a Novel Basidiomycete Strain Peniophora sp. (
  • Objectives: In this study, we described the identification of a basidiomycete isolate NDVN01 and optimization of culture conditions and medium components for CMCase production by this strain under liquid state fermentation. (
  • This protection was almost as great as that provided by direct culture on collagen IV (22% apoptotic). (
  • In 3D alginate culture, NC activated NPC either in indirect co-culture or by addition of NCCM as indicated by the gene expression ratio of aggrecan/collagen type 2. (
  • When YK-1 was grown anaerobically in a sulfur-depleted inorganic medium overlaid with crude oil, sulfate was produced, corresponding to its growth. (
  • The hybridomas can be grown indefinitely in a suitable cell culture medium. (
  • The moisture and substrates innate to the automobile air conditioning systems provided an environment suitable for microbial biofilm development and odor production. (
  • the recommended method(s) and a list of test strains suitable for assessing the quality (productivity and selectivity) of the medium and a description of the typical appearance of the target organism. (
  • Media and prospects for the cultivation of marine algae. (
  • NDVN01 was obtained at 28°C, with the initial medium pH of 7 and within 120 hours of cultivation in the optimum medium containing 80% (v/v) of potato infusion, 0.6% (w/v) straw rice as additional carbon source and 0.2% (w/v) ammonium hydrogen phosphate as an additional nitrogen source, and 0.5% (w/v) pulp as inducer. (
  • Gonadotropins promoted overall cell survival regardless of the culture medium employed. (
  • Human monocytes were isolated from PBMCs by Ficoll gradient media. (
  • Hence, this study investigated whether a combination of chemical stimuli (ascorbic acid, beta-glycerophosphate and dexamethasone) and culture media conditioned by a human foetal osteoblast cell line (hFOB) had any synergistic effect on the osteogenesis of MSC. (
  • Here we show that a combination of a microRNA (miR-124) and two transcription factors (MYT1L and BRN2) is sufficient to directly reprogram postnatal and adult human primary dermal fibroblasts (mesoderm) to functional neurons (ectoderm) under precisely defined conditions. (
  • Agar-LCM is prepared using normal human peripheral blood leukocytes in an agar-containing medium. (
  • The cardiac progenitor cell population used in the present study is isolated from explant cultures of adult human endomyocardial biopsies using an intermediate cardiosphere (CSp) step. (
  • 3. centrifuge collected medium at 1,000 g for 10mins to remove cell depris etc and store in 1ml aliquots in -80deg? (
  • Conditioned media with or without prior heat shock treatment (42°C for 1 h) of the hFOB cell line, were collected and tested on rabbit MSC cultures, in the presence and absence of chemical stimuli. (
  • Although three-dimensional testicular cell cultures have been demonstrated to mimic the organization of the testis in vivo and support spermatogenesis, the optimal culture conditions and requirements remain unknown. (
  • BAGNARA, JOSEPHT & FUKUZAWA, TOSHIHIKO 1990, ' Stimulation of Cultured Iridophores by Amphibian Ventral Conditioned Medium ', Pigment Cell Research , vol. 3, no. 5, pp. 243-250. (
  • Under normal physiological conditions, they are found in a resting state and continuously scan their microenvironments to clear cell debris and provide neurotrophic support to maintain the health of surrounding neurons ( 1 ). (
  • of media with spent culture supernatants or cell-free extracts derived from helper strains has been used for the growth stimulation of species such as Catellibacterium spp. (
  • DRG neurons were dissected from C57BL6/J mice and cultured with or without Schwann cell-conditioned media in the presence or absence of GLP-1 (7-37) or exendin-4. (
  • The aim of this study was to determine NC culture conditions (2D/3D, fetal calf serum, oxygen level) that lead to significant IVD cell activation in an indirect co-culture system under normoxia and hypoxia (2% oxygen). (
  • In vitro screening of conditioned media (CM) focused our study on three GFs, vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin-like growth factor (IGF)-1, that have been shown to play important roles after MI, 18-24 including reducing cell death, improving microcirculation and partially preventing remodeling. (
  • Quidel's prepared media can be used in conjunction with our cell-based systems. (
  • For convenience and reproducibility, a number of commercial stem cell media are commercially available in ready-to-use formulations. (
  • Your search returned 304 Media Stem Cell Cell Culture Media across 16 suppliers. (
  • The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. (
  • The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. (
  • Here, we report successful high-throughput optimization of culture medium and conditions for efficient PF production using P. fluorescens BD5. (
  • NDVN01 produced 24.65 ± 0.37 units of CMCase per mL of culture supernatant, which was 8.6 times higher than the amount (2.87 ± 0.28 U.mL -1 ) before optimization. (
  • however, at 10 degrees C and under anaerobic conditions, ECM seems to be unnecessary. (
  • To define the optimal culture conditions for your bioprocess, depending on the system, up to 24 bioreactors can be controlled individually or in parallel, to analyze the effect of varying process parameters. (
  • This provides clear evidence of a synergistic effect of conditioned media, heat shock and chemical stimuli. (
  • The intense stimulation of iridophore expression in VCM is consistent with the known inhibitory effect of this medium on melanization and with its purported role in the determination of dorsal/ventral pigment patterns of amphibians. (
  • The effect of the CM was retained in fractions that contains complex of molecules larger than 50 kDa in native condition with ultrafiltration method and disappeared by trypsin digestion. (
  • The addition of glucose to the culture diminished greatly the synthesis of a -amylase, demonstrating that a classical glucose effect is operative in this organism. (
  • These bacteria were subsequently cultured on solid media, but grew only in the presence of other bacteria, implying codependency. (
  • Similar diffusion chambers have been constructed since, to culture 'uncultivable' or rarely cultivated bacteria from marine (Nichols et al. (
  • 2009) waters have been used to culture previously uncultivated bacteria. (
  • Add fresh culture medium, aspirate and dispense into new culture flasks. (
  • Results We provide evidence by flow cytometry that monolayer culture is not favorable for NC culture with respect to maintaining NC phenotype. (
  • The optimum temperature and initial medium pH for amylase synthesis by the organism were 50°C and 7.0 respectively. (
  • Biofilms of Escherichia coli O157:H7 were developed on stainless steel chips in trypticase soy broth (TSB), 1/5 dilution of TSB, 0.1% Bacto peptone (BP) and a minimal salts medium (MSM) supplemented with 0.04% of one of the following carbon sources: glucose, glycerol, lactose, mannose, succinic acid, sodium pyruvate or lactic acid. (
  • This mineral salts medium is based on (Hartmans et al. (
  • Stanley Baran is the author of 'Introduction to Mass Communication: Media Literacy and Culture', published 2009 under ISBN 9780073378909 and ISBN 0073378909. (
  • Thus, the present study was undertaken to obtain a better understanding of ST and LT toxin production and release under laboratory conditions. (
  • On this course you will study the forces that shape the media, creative and cultural industries and gain the specialist analytic and creative skills you need to carve out a career in them. (
  • inhibition of Escherichia coli in media supplemented with 5 mM cAMP, and in a study by Chen & Brown (1985), the addition of cAMP at levels ranging from 0.01 to 100 μM showed no consistent influence on the growth rates of Legionella pneumophila. (
  • The present study deals with the isolation and identification of a bacterium and describes the effects of culture conditions on the activity of a -amylase. (
  • This study aims to investigate the role of FGF-2 in the cellular plasticity of the spinal cord in the presence of sciatic nerve-conditioned medium (SNCM) in rats. (
  • Various chemical components of the medium have fluorescence characteristics, and thus EEMs measurement is well suited to measure several components simultaneously (see attached app note for more info) . (
  • They have since evolved in a glucose-limited medium that also contains citrate, which E. coli cannot use as a carbon source under oxic conditions. (
  • For 1x medium without carbon source, pool 10 ml of each 100x stock solution and fill up to 1000 ml with sterile, deionized water and store at 4°C. (
  • Finally, SFN inhibits microglia-mediated neurotoxicity as demonstrated by conditioned medium and co-culture experiments. (
  • Influence of culture conditions on biofilm formation by Escherichia coli O157:H7. (
  • Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. (
  • One of the resulting tumors was dissociated, placed in culture and designated NL20-TA. (
  • Our current understanding of microbial growth and development stems largely from investigations conducted using single-species cultures. (
  • Consequently, an important next step in advancing our developmental understanding of microbes will be to expand our investigations to include multi-species cultures, and in doing so, unveil new and unexpected microbial growth strategies. (
  • Spinal cord injuries (SCI) usually result in crippling conditions due to the very limited repair ability of the mammalians' central nervous system (CNS). (
  • Moreover, the activity of exogenously added cellulases was not reduced in the presence of growing cultures of L. brevis . (

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