Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Culture Media, Conditioned: Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Mass Media: Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.Otitis Media: Inflammation of the MIDDLE EAR including the AUDITORY OSSICLES and the EUSTACHIAN TUBE.Culture Media, Serum-Free: CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Bacteriological Techniques: Techniques used in studying bacteria.Blood: The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.Embryo Culture Techniques: The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Kinetics: The rate dynamics in chemical or physical systems.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Social Media: Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.Organ Culture Techniques: A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Blastocyst: A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Peptones: Derived proteins or mixtures of cleavage products produced by the partial hydrolysis of a native protein either by an acid or by an enzyme. Peptones are readily soluble in water, and are not precipitable by heat, by alkalis, or by saturation with ammonium sulfate. (Dorland, 28th ed)Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Communications Media: The means of interchanging or transmitting and receiving information. Historically the media were written: books, journals, newspapers, and other publications; in the modern age the media include, in addition, radio, television, computers, and information networks.Molecular Weight: The sum of the weight of all the atoms in a molecule.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Bacterial Proteins: Proteins found in any species of bacterium.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Fertilization in Vitro: An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Amino Acids: Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.Contrast Media: Substances used to allow enhanced visualization of tissues.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Embryonic and Fetal Development: Morphological and physiological development of EMBRYOS or FETUSES.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Microbial Collagenase: A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC 3.4.24.3.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Anaerobiosis: The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Osmolar Concentration: The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.Proteoglycans: Glycoproteins which have a very high polysaccharide content.Glycosaminoglycans: Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Aerobiosis: Life or metabolic reactions occurring in an environment containing oxygen.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Cycloheximide: Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Ectogenesis: Embryonic and fetal development that takes place in an artificial environment in vitro.Chromatography, High Pressure Liquid: Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Sulfates: Inorganic salts of sulfuric acid.Otitis Media, Suppurative: Inflammation of the middle ear with purulent discharge.Sodium Chloride: A ubiquitous sodium salt that is commonly used to season food.Tissue Culture Techniques: A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.Chromogenic Compounds: Colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds; used in biochemical assays and in diagnosis as indicators, especially in the form of enzyme substrates. Synonym: chromogens (not to be confused with pigment-synthesizing bacteria also called chromogens).Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Growth Substances: Signal molecules that are involved in the control of cell growth and differentiation.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Acetates: Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.HEPES: A dipolar ionic buffer.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Embryonic Development: Morphological and physiological development of EMBRYOS.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Oocytes: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Sucrose: A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.Polyanetholesulfonate: A compound originally developed as an anticoagulant, but possessing anticomplement action and lowering the bactericidal action of blood. It is used in vitro to inhibit blood coagulation and as a diagnostic reagent to encourage the growth of pathogens in the blood. It is also used to stabilize colloidal solutions such as milk and gelatin. (From Merck Index, 11th ed)Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Fungi: A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.Bioreactors: Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.TritiumSulfur Radioisotopes: Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Enzyme Induction: An increase in the rate of synthesis of an enzyme due to the presence of an inducer which acts to derepress the gene responsible for enzyme synthesis.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Fallopian Tubes: A pair of highly specialized muscular canals extending from the UTERUS to its corresponding OVARY. They provide the means for OVUM collection, and the site for the final maturation of gametes and FERTILIZATION. The fallopian tube consists of an interstitium, an isthmus, an ampulla, an infundibulum, and fimbriae. Its wall consists of three histologic layers: serous, muscular, and an internal mucosal layer lined with both ciliated and secretory cells.Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Morula: An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Embryo Transfer: The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.Colony Count, Microbial: Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Tunica Media: The middle layer of blood vessel walls, composed principally of thin, cylindrical, smooth muscle cells and elastic tissue. It accounts for the bulk of the wall of most arteries. The smooth muscle cells are arranged in circular layers around the vessel, and the thickness of the coat varies with the size of the vessel.Phosphates: Inorganic salts of phosphoric acid.Epithelium: One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Extracellular Space: Interstitial space between cells, occupied by INTERSTITIAL FLUID as well as amorphous and fibrous substances. For organisms with a CELL WALL, the extracellular space includes everything outside of the CELL MEMBRANE including the PERIPLASM and the cell wall.Metalloendopeptidases: ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Pichia: Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.Mycelium: The body of a fungus which is made up of HYPHAE.Oxidation-Reduction: A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).GlucosamineL-Lactate Dehydrogenase: A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.Rats, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Interleukin-1: A soluble factor produced by MONOCYTES; MACROPHAGES, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Interleukin-1 is a general term refers to either of the two distinct proteins, INTERLEUKIN-1ALPHA and INTERLEUKIN-1BETA. The biological effects of IL-1 include the ability to replace macrophage requirements for T-cell activation.Genes, Bacterial: The functional hereditary units of BACTERIA.Serum: The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.Tissue Preservation: The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Cell Adhesion: Adherence of cells to surfaces or to other cells.Methionine: A sulfur-containing essential L-amino acid that is important in many body functions.Microbiological Techniques: Techniques used in microbiology.Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.Leucine: An essential branched-chain amino acid important for hemoglobin formation.Charcoal: An amorphous form of carbon prepared from the incomplete combustion of animal or vegetable matter, e.g., wood. The activated form of charcoal is used in the treatment of poisoning. (Grant & Hackh's Chemical Dictionary, 5th ed)Mitosporic Fungi: A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.Glycerol: A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Rats, Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Industrial Microbiology: The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.Solubility: The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Biological Assay: A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.Lymphocytes: White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.Polysorbates: Sorbitan mono-9-octadecanoate poly(oxy-1,2-ethanediyl) derivatives; complex mixtures of polyoxyethylene ethers used as emulsifiers or dispersing agents in pharmaceuticals.Cell Line, Tumor: A cell line derived from cultured tumor cells.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Cell SeparationHypertonic Solutions: Solutions that have a greater osmotic pressure than a reference solution such as blood, plasma, or interstitial fluid.Estradiol: The 17-beta-isomer of estradiol, an aromatized C18 steroid with hydroxyl group at 3-beta- and 17-beta-position. Estradiol-17-beta is the most potent form of mammalian estrogenic steroids.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.Oxygen: An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.Zygote: The fertilized OVUM resulting from the fusion of a male and a female gamete.Eye Banks: Centers for storing various parts of the eye for future use.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Granulosa Cells: Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH).Endothelium: A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.Cartilage: A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.Mycology: The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.Potassium: An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.Blood Physiological Phenomena: Physiological processes and properties of the BLOOD.Gelatin: A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.Incubators: Insulated enclosures in which temperature, humidity, and other environmental conditions can be regulated at levels optimal for growth, hatching, reproduction, or metabolic reactions.Progesterone: The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS.Transferrin: An iron-binding beta1-globulin that is synthesized in the LIVER and secreted into the blood. It plays a central role in the transport of IRON throughout the circulation. A variety of transferrin isoforms exist in humans, including some that are considered markers for specific disease states.Chromatography, Thin Layer: Chromatography on thin layers of adsorbents rather than in columns. The adsorbent can be alumina, silica gel, silicates, charcoals, or cellulose. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Mycoplasma: A genus of gram-negative, mostly facultatively anaerobic bacteria in the family MYCOPLASMATACEAE. The cells are bounded by a PLASMA MEMBRANE and lack a true CELL WALL. Its organisms are pathogens found on the MUCOUS MEMBRANES of humans, ANIMALS, and BIRDS.Carbohydrate Metabolism: Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.Insulin: A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Dactinomycin: A compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy. (From AMA Drug Evaluations Annual, 1993, p2015)Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Ear, Middle: The space and structures directly internal to the TYMPANIC MEMBRANE and external to the inner ear (LABYRINTH). Its major components include the AUDITORY OSSICLES and the EUSTACHIAN TUBE that connects the cavity of middle ear (tympanic cavity) to the upper part of the throat.Endothelium, Vascular: Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.Endopeptidases: A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.Carbon Isotopes: Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.ThymidinePolymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Carbon: A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.Fatty Acids: Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)Thioglycolates: Organic esters of thioglycolic acid (HS-CH2COOH).Bucladesine: A cyclic nucleotide derivative that mimics the action of endogenous CYCLIC AMP and is capable of permeating the cell membrane. It has vasodilator properties and is used as a cardiac stimulant. (From Merck Index, 11th ed)Dexamethasone: An anti-inflammatory 9-fluoro-glucocorticoid.Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.Nitrogen: An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.Staphylococcus: A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.Fibronectins: Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.Lipopolysaccharides: Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)Glycoside Hydrolases

A simple technique for mass cultivation of Campylobacter fetus. (1/23774)

Studies using 86 media for maximum growth of Campylobacter fetus for antigen production showed that a diphasic medium (solid base with liquid overlay) was most suitable. The solid base was double strength cystine heart agar. The liquid overlay was thioglycollate medium of Brewer (135-C) without agar. This medium yielded maximum growth of C. fetus in six days with good motility, less clumping and less filament formation than all other media tried.  (+info)

Stimulation of thymidine uptake and cell proliferation in mouse embryo fibroblasts by conditioned medium from mammary cells in culture. (2/23774)

Undialyzed conditioned medium from several cell culture sources did not stimulate thymidine incorporation or cell overgrowth in quiescent, density-inhibited mouse embryo fibroblast cells. However, dialyzed conditioned medium (DCM) from clonal mouse mammary cell lines MCG-V14, MCG-T14, MCG-T10; HeLa cells; primary mouse adenocarcinoma cells; and BALB/c normal mouse mammary epithelial cells promoted growth in quiescent fibroblasts. The amount of growth-promoting activity produced per cell varied from 24% (HeLa) to 213% (MCG-V14) of the activity produced by primary tumor cells. The production of growth-promoting activity was not unique to tumor-derived cells or cells of high tumorigenicity. The amount of growth-promoting activity produced per cell in the active cultures was not correlated with any of the following: tumorigenicity, growth rat, cell density achieved at saturation, cell type, or species of cell origin. It is concluded that transformed and non-transformed cells of diverse origin, cell type, and tumorigenicity can produce growth factors in culture. The growth-promoting potential of the active media from primary tumor cultures accumulated with time of contact with cells and was too great to be accounted for entirely by the removal of low-molecular-weight inhibitors by dialysis. The results are consistent with the hypothesis that conditioned medium from the active cultures contained a dialyzable, growth-promoting activity. Different cell lines exhibited differential sensitivity to tumor cell DCM and fetal bovine serum. Furthermore, quiescent fibroblasts were stimulated by primary tumor cell DCM in the presence of saturating concentrations of fetal bovine serum. These observations support the notion that the active growth-promoting principle in primary tumor cell DCM may not be a serum factor(s).  (+info)

Improved medium for recovery and enumeration of Pseudomonas aeruginosa from water using membrane filters. (3/23774)

A modified mPA medium, designated mPA-C, was shown to recover Pseudomonas aeruginosa from a variety of water sources with results comparable to those with mPA-B and within the confidence limits of a most-probable-number technique. Enumeration of P. aeruginosa on mPA-C was possible after only 24 h of incubation at 41.5 degrees C, compared with 72 h of incubation required for mPA-B and 96 h of incubation for a presumptive most probable number.  (+info)

Unsaturated fatty acid requirements for growth and survival of a rat mammary tumor cell line. (4/23774)

A cell line, the growth and survival of which is markedly affected by linoleic acid, has been established from a carcinogen-induced rat mammary tumor. The cells have been continuously passaged in 5% rat serum plus 10% fetal calf serum-supplemented medium. The rat serum component was found to be indispensalbe, for when it was omitted the growth rate rapidly declined and the cells died by 5 to 7 days. Removal of the rat serum from the growth medium also resulted in a dramatic loss of Oil Red O-positive droplets in the cells, suggesting that the lipid component of rat serum might be a major growth-promoting principle in rat serum. This is likely since the total lipid fraction, but not the delipidized protein fraction, could largely supplant requirement of the cells for rat serum. Pure linoleic acid was found to be effective in maintaining the cell growth in delipidized serum or in whole fetal calf serum-supplemented medium. Fatty acid analysis revealed a 19-fold higher amount of linoleic acid in rat serum than in fetal calf serum.  (+info)

Nrg1 is a transcriptional repressor for glucose repression of STA1 gene expression in Saccharomyces cerevisiae. (5/23774)

Expression of genes encoding starch-degrading enzymes is regulated by glucose repression in the yeast Saccharomyces cerevisiae. We have identified a transcriptional repressor, Nrg1, in a genetic screen designed to reveal negative factors involved in the expression of STA1, which encodes a glucoamylase. The NRG1 gene encodes a 25-kDa C2H2 zinc finger protein which specifically binds to two regions in the upstream activation sequence of the STA1 gene, as judged by gel retardation and DNase I footprinting analyses. Disruption of the NRG1 gene causes a fivefold increase in the level of the STA1 transcript in the presence of glucose. The expression of NRG1 itself is inhibited in the absence of glucose. DNA-bound LexA-Nrg1 represses transcription of a target gene 10.7-fold in a glucose-dependent manner, and this repression is abolished in both ssn6 and tup1 mutants. Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro. These findings indicate that Nrg1 acts as a DNA-binding repressor and mediates glucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex.  (+info)

Interaction of inflammatory cells and oral microorganisms. II. Modulation of rabbit polymorphonuclear leukocyte hydrolase release by polysaccharides in response to Streptococcus mutans and Streptococcus sanguis. (6/23774)

The release of lysosomal hydrolases from polymorphonuclear leukocytes (PMNs) has been postulated in the pathogenesis of tissue injury in periodontal disease. In the present study, lysosomal enzyme release was monitored from rabbit peritoneal exudate PMNs exposed to Streptocccus mutans or Streptococcus sanguis. S. mutans grown in brain heart infusion (BHI) broth failed to promote significant PMN enzyme release. S. sanguis grown in BHI broth, although more effective than S. mutants, was a weak stimulus for promotion of PMN hydrolase release. Preincubation of washed, viable S. mutans in sucrose or in different-molecular-weight dextrans resulted in the ability of the organisms to provoke PMN release reactions. This effect could bot be demonstrated with boiled or trypsinized S. mutans or with viable S. sanguis. However, when grown in BHI broth supplemented with sucrose, but not with glucose, both S. mutans and S. sanguis triggered discharge of PMN enzymes. The mechanism(s) whereby dextran or sucrose modulates PMN-bacterial interaction may in some manner be related to promotion of microbial adhesiveness or aggregation by dextran and by bacterial synthesis of glucans from sucrose.  (+info)

Downregulation of metallothionein-IIA expression occurs at immortalization. (7/23774)

Metallothioneins (MTs) may modulate a variety of cellular processes by regulating the activity of zinc-binding proteins. These proteins have been implicated in cell growth regulation, and their expression is abnormal in some tumors. In particular, MT-IIA is expressed 27-fold less in human colorectal tumors and tumor cell lines compared with normal tissue (Zhang et al., 1997). Here we demonstrate that MT-IIA downregulation occurs when human cells become immortal, a key event in tumorigenesis. After immortalization MT-IIA expression remains inducible but the basal activity of the MT-IIA promoter is decreased. MT-IIA downregulation at immortalization is one of the most common immortalization-related changes identified to date, suggesting that MT-IIA has a role in this process.  (+info)

Estrogen-dependent and independent activation of the P1 promoter of the p53 gene in transiently transfected breast cancer cells. (8/23774)

Loss of p53 function by mutational inactivation is the most common marker of the cancerous phenotype. Previous studies from our laboratory have demonstrated 17 beta estradiol (E2) induction of p53 protein expression in breast cancer cells. Although direct effects of E2 on the expression of p53 gene are not known, the steroid is a potent regulator of c-Myc transcription. In the present studies, we have examined the ability of E2 and antiestrogens to regulate the P1 promoter of the p53 gene which contains a c-Myc responsive element. Estrogen receptor (ER)-positive T47D and MCF-7 cells were transiently transfected with the P1CAT reporter plasmid and levels of CAT activity in response to serum, E2 and antiestrogens were monitored. Factors in serum were noted to be the dominant inducers of chloramphenicol acetyltransferase (CAT) expression in MCF-7 cells. The levels of CAT were drastically reduced when cells were maintained in serum free medium (SFM). However, a subtle ER-mediated induction of CAT expression was detectable when MCF-7 cells, cultured in SFM, were treated with E2. In serum-stimulated T47D cells, the CAT expression was minimal. The full ER antagonist, ICI 182 780 (ICI) had no effect. Treatment with E2 or 4-hydroxy tamoxifen (OHT) resulted in P1CAT induction; OHT was more effective than E2. Consistent with c-Myc regulation of the P1 promoter, E2 stimulated endogenous c-Myc in both cell lines. Two forms of c-Myc were expressed independent of E2 stimuli. The expression of a third more rapidly migrating form was E2-dependent and ER-mediated since it was blocked by the full ER antagonist, ICI, but not by the ER agonist/antagonist OHT. These data demonstrate both ER-mediated and ER-independent regulation of c-Myc and the P1 promoter of the p53 gene, and show differential effects of the two classes of antiestrogens in their ability to induce the P1 promoter of the p53 gene in breast cancer cells.  (+info)

*Body Positive Movement

"How social media is changing the feminist movement". MSNBC. Retrieved 2017-11-28. "How social media is changing the feminist ... Body positivity is about radically re-imaging how American culture views bodies, moving from a society where differences are ... The article, Feminism and the Social Media Sphere by Mereen Kasana, describes social media as systems of feminist spaces where ... Social networking has become an influential medium to support civic and social justice. Studies show the power of social media ...

*Ham's tissue culture medium

Murashige and Skoog medium (Plant cell culture medium) RPMI (Roswell Park Memorial Institute medium), for lymph cells The ... Ham's tissue culture medium is a growth medium for mammalian cells. It contains in amounts dissolved in 1 liter of triple ... a minimum ingredient cell culture medium) Fermentation (food) GMEM (Glasgow's Minimal Essential Medium) ... 1.3 mg NaHCO3 1.2 g Biotechnology Brewing Cell biology DMEM Dulbecco's Modified Eagle's Medium Eagle's minimal essential medium ...

*Psychology of Popular Media Culture

... is a peer-reviewed academic journal published by the American Psychological Association. It ... "Psychology of Popular Media Culture". American Psychological Association. October 24, 2012. Retrieved 2012-10-24. Official ... "how popular culture and general media influence individual, group, and system behavior." The founding editors were James C. ...

*Digital, Culture, Media and Sport Committee

The Digital, Culture, Media and Sport Committee, formerly the Culture, Media and Sport Committee, is one of the Select ... It oversees the operations of the Department for Digital, Culture, Media and Sport which replaced the Department for Culture, ... Media and Sport Committee". UK Parliament. Retrieved 18 September 2017. "Digital, Culture, Media and Sport Committee membership ... The Committee currently comprises: Source: Culture, Media and Sport Committee Members in the previous 2014/15 session of ...

*Media, Culture & Society

... is a peer-reviewed academic journal that covers media studies. The editors-in-chief are Raymond Boyle ... "Media, Culture & Society". 2013 Journal Citation Reports. Web of Science (Science ed.). Thomson Reuters. 2014. Official website ...

*Crime, Media, Culture

Crime Media Culture is a peer-reviewed academic journal covering research on the relationship between crime, criminal justice, ... media, and culture. The editors-in-chief are Chris Greer (City University London) and Mark S. Hamm (Indiana State University). ...

*Secretary of State for Digital, Culture, Media and Sport

Her Majesty's Principal Secretary of State for Digital, Culture, Media and Sport, or informally Culture Secretary, is a United ... In 2017 the DCMS was renamed to the Department for Digital, Culture, Media and Sport in acknowledgement of the increasing ... Shadow Secretary of State for Culture, Media and Sport Townsend, Nick (17 January 1999). "Interview: David Mellor-A more mellow ... Kingdom cabinet position with responsibility for the Department for Digital, Culture, Media and Sport (DCMS). The role was ...

*Shadow Secretary of State for Digital, Culture, Media and Sport

The Shadow Secretary of State for Digital, Culture, Media and Sport, previously Shadow Secretary of State for Culture, Media ... Media and Sport and junior ministers to account and is the lead spokesperson for his or her party on culture, media and sport ... as has indeed the Department for Culture, Media and Sport). Official Opposition frontbench. ... Should the relevant party take office, the Shadow Secretary would be a likely candidate to become Culture Secretary. The post ...

*Culture and Media Institute

"MRC Launches Culture & Media Institute" (Press release). Media Research Center. 2006-10-18. "Eye on Culture Archive". CMI. ... The Culture and Media Institute (CMI) is a conservative American non-profit organization focusing on promoting what it ... "Eye on Culture" is a regular feature in which CMI writers express their opinions on American popular culture. Among its ... It was founded in October 2006 as a division of the Media Research Center, a group that monitors perceived liberal news media ...

*Fastidious organism

Growth medium Rao P.N., Sridhar. "Culture Media" (PDF). Retrieved 23 March 2012. Todar, Kenneth. Neisseria gonorrhoeae, the ... field of microbiology to describe microorganisms that will grow only if special nutrients are present in their culture medium. ...

*On My Own (Yasmin song)

Edwards Byfield, Tahirah (11 November 2010). "Yasmin - 'On My Own'". Soul Culture. Soul Culture Media. Archived from the ... Edwards Byfield, Tahirah (3 December 2010). "Yasmin - 'On My Own'". Soul Culture. Soul Culture Media. Archived from the ... Writer, Staff (18 July 2011). "'On My Own' places UK's Yasmin On The Rise". Aslan Media Music. Aslan Media. Archived from the ... Guardian Media Group. Archived from the original on 17 June 2013. Retrieved 17 June 2013. MistaJam (16 November 2010). " ...

*Wynonna Earp

Pop Culture Media. Dickens, Donna (October 4, 2016). Interview: How Syfy reinvigorated the 'Wynonna Earp' creator to expand his ... Pop Culture Media. Retrieved July 1, 2017. Melanie Scrofano @MelanieScrofano (October 18, 2016). "I shall be INSUFFERABLE when ...

*Here I Am (Monica song)

Soul Culture Media. Retrieved 2013-02-01. "Here I Am (Remix): Monica: MP3 Downloads". Amazon.com. Retrieved 2010-10-22. "Monica ... Media notes). Monica. J Records. 2010. "Rap-Up TV Interviews Monica: Part 1". Rap-Up. SPIN Music Group. BUZZMEDIA. 2010-10-20. ...

*Summer Block Party

"Jill Scott - 'Blessed'". Soul Culture. Soul Culture Media. 24 April 2012. Archived from the original on 22 May 2012. Retrieved ... Cumulus Media. April 2012. Archived from the original on 22 May 2012. Retrieved 13 May 2012. Soref, Dave (6 June 2011). "Jill ... Calkins Media. April 2012. Archived from the original on 22 May 2012. Retrieved 13 May 2012. Lipshutz, Jason (2 June 2011). " ...

*European Committees

Culture Media and Sport; Health The committee debates and votes on an amendable motion with respect to the document, with the ...

*Samurai in Japanese literature

"Wish To Master "How To Fight?" Know These Japanese Literature On Military Strategy". Wasabi - Japanese Culture Media. Retrieved ... its innate clarity has allowed it to be readily accessible to readers across the ages and across cultures. It is said the ...

*Round-bottom flask

Storage the culture media. Preparation of gas-phase standards for flasks fitted with septa (requires volumetric calibration) ...

*Robert Crawford (historian)

Department for Culture Media & Sport. June 2013. Retrieved 26 April 2017. Imperial War Museum. ... Guardian Media. 3 July 2014. p. 33. ,access-date= requires ,url= (help) "Nisha Katona and Sir Robert Crawford CBEreappointedas ...

*Reviewing Committee on the Export of Works of Art

"UK Export Licensing For Cultural Goods" (PDF). Department for Culture, Media and Sport. 28 February 2003. Archived from the ... "Export of Objects of Cultural Interest 2011/12" (PDF). Department for Culture, Media and Sport. December 2012. Retrieved 8 ... Culture, Media & Sport. 6 March 2014. Retrieved 8 October 2017. Official website Harwood, Richard (2004). "The Work of the ... advising the Department of Culture, Media and Sport (DCMS) on the export of cultural property. Some of its roles were shifted ...

*William Lowndes (1652-1724)

Department for Culture Media & Sport. Retrieved 7 January 2017. "Portrait by Phillips". ArtUK. ArtUK. Retrieved 7 January 2017 ...

*Middlesbrough Roller Derby

"2012 Grants - Middlesbrough". Department for Culture Media & Sport. Retrieved 17 April 2017. "WFTDA Welcomes 16 Apprentice ...

*Francis Jukes

Department for Digital, Culture Media & Sport. Retrieved 20 September 2017. Finberg, Alexander Joseph; Holme, Charles; Taylor, ... Francis James Sarjent Media related to Francis Jukes at Wikimedia Commons Francis Jukes artwork Oxford dictionary of National ...

*Mila Rechcigl

G. Diets, Culture Media, Food Supplements. Vol. 4. Culture Media for Cells, Organs and Embryos. Cleveland, OH: CRC Press, 1977 ... G. Diets, Culture Media, Food Supplements. Vol. 3. Culture Media for Microorganisms and Plants. Cleveland, OH: CRC Press, 1978 ... G. Diets, Culture Media, Food Supplements. Vol. 1. Dits for Mammals. Cleveland, OH: CRC Press, 1977. 645 pp. CRC Handbook ... G. Diets, Culture Media, Food Supplements. Vol. 2. Food Habits of and Diets for Invertebrates and Vertebrates. Zoo diets. ...

*Bath Fringe Festival

"Grant Distribution". Department for Culture Media and Sports. Retrieved 22 August 2011. Festival website Historical Walcot ...

*National Museums of the United Kingdom

Department for Culture Media and Sport. "Sponsored Institutions". DCMS. Retrieved 1 June 2012. List of museums in England List ... The national museums of the UK are funded by the Department for Culture, Media and Sport (DCMS) of the United Kingdom ... "Department for Culture, Media and Sport - Sponsored Museums and Galleries". Retrieved 2007-08-01. "National Museums of Scotland ... of museums in Scotland List of museums in Wales List of museums in Northern Ireland Department for Culture, Media and Sport: ...

*George Finlayson

1]. Henry Ginsburg Thai art and culture: historic manuscripts from western collections. University of Hawaii Press, 2000 ISBN 0 ... Adamant Media. p. 251. ISBN 0-543-92398-3. Ripley, George; Dana, Charles A., eds. (1879). "Finlayson, George". The American ...

*Valentine Green

Department for Digital, Culture Media & Sport. Retrieved 20 September 2017. Green, Valentine (1764). A Survey of the City of ...
|p||strong|Technical Advantage:|/strong|  Product manufactured under industry-leading standards for low endotoxin content and superior results|/p| |p||br /| Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.  This research indicated that additions made to BME aided cell propagation.  MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells.  When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts.  This formulation contains Earles salts and does not contain L-glutamine, phenol red, and sodium bicarbonate.|/p| |p||br /| -  Complete Certificate of Analysis available for each production lot, along with full formulation information|/p|
|p||strong|Technical Advantage: |/strong| Product manufactured under industry-leading standards for low endotoxin content and superior results.|br /| Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.  This research indicated that additions made to BME aided cell propagation.  MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells.  When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts.  This formulation contains Earles salts and does not contain L-glutamine and phenol red.|br /| -  Complete Certificate of Analysis available for each production lot, along with full formulation information|/p|
Tissue culture. Dissociated mixed neocortical cell suspensions were prepared from fetal Swiss-Webster mice (gestational age 14-16 d) and plated on a previously established confluent layer of cortical astrocytes (see below) in 24-well tissue culture plates at 1-2 × 105 cells/cm2, generally as described previously (Rose et al., 1993). The initial plating medium was Eagles Minimum Essential Medium (MEM; Earles salts, supplied glutamine-free) supplemented with 10% heat-inactivated horse serum, 10% heat-inactivated fetal bovine serum, glutamine (total, 2 mm), and glucose (total, 21 mm). Cultures were kept in a 37°C/5% CO2 incubator. After 4-6 d in vitro, non-neuronal cell division was halted by exposure to 10−5 m cytosine arabinoside for 1-3 d. The cells were then shifted into a maintenance medium identical to the plating medium but lacking fetal serum. Subsequent media replacement occurred twice a week. Cultures were studied after 13-14 d in vitro.. Glial cultures were prepared using the same ...
Sibling oocytes will be equally divided and incubated separately in different 4-well culture dishes containing either IVF medium or ISM1 Medium and while ICSI will be returned to the same culture dish following injection. Fertilization assessment will be performed approximately after 15-20 hours for standard IVF and 12-18 hours post ICSI performance. After assessment, fertilized oocytes from different culture media will be transferred separately into fresh culture dishes containing ISM1 medium only. The zygotes will be incubated until embryo transfer on day 2 or 3. Embryo quality will be assessed according to the morphology of blastomeres as well as its developmental stage ...
Specimen preferred to arrive within 96 hours of collection.. Submit only 1 of the following specimens:. Preferred:. Specimen Type: Whole blood. Container/Tube:. Preferred: Lavender top (EDTA) or yellow top (ACD). Acceptable: Any anticoagulant. Specimen Volume: 3 mL. Collection Instructions:. 1. Invert several times to mix blood.. 2. Send specimen in original tube.. Specimen Stability Information: Ambient (preferred)/Refrigerated. Specimen Type: Cultured fibroblasts. Container/Tube: T-75 or T-25 flask Specimen Volume: 1 Full T-75 or 2 full T-25 flasks. Specimen Stability Information: Ambient (preferred)/Refrigerated ,24 hours. Specimen Type: Skin biopsy. Container/Tube: Sterile container with any standard cell culture media (eg, minimal essential media, RPMI 1640). The solution should be supplemented with 1% penicillin and streptomycin. Tubes can be supplied upon request (Eagles minimum essential medium with 1% penicillin and streptomycin [T115]).. Specimen Volume: 4-mm punch Specimen Stability ...
Article SEL, a selective enrichment broth for simultaneous growth of salmonella enterica, escherichia coli o157:h7, and listeria monocytogenes. Multipathogen detection on a single-assay platform not only reduces the cost for testing but also provides...
The rates of uridine-5-3H incorporation into RNA and the rates of uridine uptake into the acid-soluble pool during the cell cycle of V79 Chinese hamster cells were examined. Cells cultured on Eagles minimal essential medium supplemented with fetal calf serum, lactalbumin hydrolysate, glutamine, and trypsin displayed rates of incorporation and uptake which increased only slightly during G1 and accelerated sharply as DNA synthesis commenced. In contrast, cells cultured on minimal essential medium supplemented only with calf serum exhibited rates of incorporation and uptake which increased linearly through both G1 and S. The transition from one pattern to the other can be induced within 24 hr and is completely reversible. The nonlinear pattern exhibited by cells grown on the supplemented fetal calf serum medium can also be overcome with high exogenous uridine concentrations. In the presence of 200 µM uridine, these cells display a linear pattern of increase in rates of uridine incorporation and uptake.
The effects of extracellular folate concentration on intracellular folate and phosphoribosylpyrophosphate (PRPP) levels and the cytotoxicity of methotrexate and 5-fluorouracil were studied in human KB cells grown in fetal bovine serum-supplemented Eagles minimum essential medium, which contained standard high folic acid levels (2.3 µm) (standard or S medium), or folic acid-free serum-supplemented medium containing approximately 4 nm 5-methyltetrahydrofolate (physiological or P medium), a folate level and form more comparable to that in normal human serum. Macrocytosis and prolongation of the doubling time by 150% were observed after 5-10 serial passes in P medium, but after 10-15 serial passes, KB cells became "adapted" to P medium with return of size and doubling time to values indistinguishable from cells maintained in S medium. Cellular folate levels fell, and marked elevations in PRPP levels from 68 ± 43 to 642 ± 287 pmol/mg cell protein (mean ± SD) were observed as KB cells were ...
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Dulbeccos Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagles Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate ...
Dulbeccos Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagles Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate ...
Sigma-Aldrich offers Sigma-M2279, Minimum Essential Medium Eagle for your research needs. Find product specific information including CAS, MSDS, protocols and references.
In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor media conditions. Other supplements such as amino acids and vitamins are useful for enriched media beyond their normal concentrations. Attachment factors and transport factors are added to growth media to facilitate rapid cell propagation and enhance proper cell metabolism. Still other media supplements may be added to create conditions which will elicit specific cellular responses and effects, such as altered protein biosynthesis, accelerated antibody production and secretion, toxic response mechanisms, etc. ...
Shionogi carcinoma 115 (SC115) had been accepted for 20 years as an androgen-dependent mouse mammary tumor, the growth of which is stimulated only by androgen. However, we very recently found that the growth of SC115 tumors in vivo is stimulated not only by physiological doses of androgen but also by pharmacological doses of estrogen through the estrogen receptor system. In the present study, the growth-stimulative effect of estrogen on an androgen-dependent cloned cell line (SC-3) derived from SC115 cells, which showed androgen- and estrogen-dependent growth in vivo, was examined in vitro. In serum-supplemented medium (Eagles minimum essential medium containing 2% steroid-free fetal calf serum), testosterone or 5α-dihydrotestosterone (10-9-10-6 m) significantly stimulated the growth of SC-3 cells (3.2-fold increase in cell number at day 10 in culture containing 10-8 m androgens) and changed the shape of SC-3 cells from epithelial to spindle (fibroblast-like), whereas 17β-estradiol ...
The Film and Media Culture department is dedicated to the study of film and visual media as a vital aspect of a liberal arts education. Our students study film and a wide range of visual media, including television, digital games, online video, and video art, as aesthetic forms as well as in relation to the cultures that produced them.. While we are not a production-centered department or a traditional "film school," we fully integrate the creation and critical study of film and media in a manner appropriate to a liberal arts education. Students will balance the critical studies of film and media with the creation of media work, often within the same course.. The faculty in Film and Media Culture are active scholars and creators, writing books, creating videos, and contributing to the vibrant academic field of film and media studies. Our classes incorporate numerous screenings of films and media texts, with scheduled evening sessions and hands-on lab sections. We are housed in the new Axinn ...
Materials. U0126 and PD98059, two specific inhibitors of the ERK1/2 MAPK pathway, and SB202190, a specific inhibitor of the p38 MAPK pathway, were purchased from Calbiochem (VWR International, Fontenay-sous-bois, France). U0126, PD98059, and SB202190 were dissolved in dimethyl sulfoxide and used at final concentration of 10 μM, as described previously (Muselet-Charlier et al., 2007). SP600125, a selective inhibitor of JNK catalytic activity (Bennett et al., 2001), and recombinant human tumor necrosis factor α (TNF-α) were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France) and used at a final concentration of 3 μM and 10 ng/ml, respectively. Eagles minimum essential medium with Earles salts, penicillin-streptomycin, and l-glutamine were purchased from Invitrogen SARL (Cergy Pontoise, France). Fetal bovine serum was purchased from Eurobio (Courtaboeuf, France).. Cell Culture. The human CF bronchial epithelial cell lines IB3-1 and CFBE41o- were used. IB3-1 was a bronchial ...
Definition : Selective culture media in the form of liquids, solids, or semisolids used as a medium capable of supporting the growth of mycoplasma, while inhibiting the growth of other microorganisms. These selective culture media combine a rich nutritive base usually containing peptones, serum (e.g. horse serum, swine serum), growth factors favoring the growth of mycoplasmas and an antibiotic mixture inhibiting the growth of most antagonistic associated bacteria. Mycoplasma selective culture media are often used for the isolation and identification of mycoplasma in pharmaceutical products, especially in bulk vaccines, cell banks, and virus cultures.. Entry Terms : "Microbiological Culture Media, Selective, Mycoplasma" , "Culture Media, Mycoplasma" , "Reagents, Microbiology, Bacteria, Culture Media, Selective, Mycoplasma". UMDC code : 17019 ...
Internet-Draft Content-Type Processing Model May 2009 1. Introduction The HTTP Content-Type header indicates the media type of an HTTP response. However, many HTTP servers supply a Content-Type that does not match the actual contents of the response. Historically, web browsers have been tolerated these servers by examining the content of HTTP responses in addition to the Content-Type header to determine the effective media type of the response. Without a clear specification of how to "sniff" the media type, each user agent implementor was forced to reverse engineer the behavior of the other user agents and to developed their own algorithm. These divergent algorithms have lead to a lack of interoperability between user agents and to security issues when the server intends an HTTP response to be interpreted as one media type but some user agents interpret the responses as another media type. These security issues are most severe when an "honest" server lets potentially malicious users upload files ...
Tryptic Soy Broth is widely used for the isolation of bacteria from clinical specimens, supporting the growth of a majority of pathogenic bacteria. This medium may be used for preparing dilutions of organism for colony counts, preparing standard inocula, and antibiotic sensitivity testing for both fastidious and non-fastidious microorganisms. Tryptic Soy Broth is also recommended for use in sterility testing for the detection of contamination with low incidence fungi and aerobic bacteria. (7) The concentration of ingredients in Tryptic Soy Broth, DS is critical to the media performance. When used to detect organisms in samples, the volume of sample being tested must be equal to the initial volume of TSB, DS. This will ensure that the ingredients in the media will be at the correct concentration after the addition of the test sample. Tryptic Soy Broth, also known as Soybean-Casein Digest, conforms to the formula given by the U.S. Pharmacopeia. (7) It was originally developed for testing the ...
Cell Culture. The established malignant glioma cell lines U87, T98G, A172, human pulmonary fibroblasts, and human umbilical vein endothelial cells (HUVECs) were obtained from the American Type Culture Collection (Manassas, VA). Human astrocytes and human cerebellar astrocytes were obtained from ScienCell Research Laboratories (San Diego, CA). LN18, LNZ308, and LNZ428 were generously provided by Dr. Nicolas de Tribolet. U87, T98G, and human pulmonary fibroblasts were cultured in growth medium composed of minimum essential medium supplemented with sodium pyruvate and nonessential amino acids; A172, LN18, LNZ308, and LNZ428 were cultured in α-minimal essential medium supplemented with l-glutamine; human astrocytes were cultured in astrocyte growth medium; and HUVECs were cultured in endothelial cell medium (ScienCell Research Laboratories). All growth media contained 10% fetal calf serum, l-glutamine, ribonucleosides, deoxynucleosides, 100 IU/ml penicillin, 100 mg/ml streptomycin, and 0.25 mg/ml ...
In our initial experiments, dissociated cells were plated into dishes whose bottom was a glass coverslip. A styryl dye stained cells and adsorbed to the glass coverslip. Unbinding of the dye from the coverslip was very slow. Quantitative measurement of vesicle cycling was difficult when images were contaminated by a large background signal. Fortunately, unbinding of the dye was much faster when cells were plated onto a coverslip that had been coated with a thin layer of silicone plastic (Sylgard 184; Dow Corning, Midland, MI). Cells adhered to the silicone plastic if it was first covered with 0.25 mg ml−1 concanavalin A in 1 m NaCl for 20 min, rinsed with distilled water, and air dried.. The extracellular saline contained (in mm): NaCl, 120; KCl, 2.5; CaCl2, 0.2; MgCl2, 1; Na-pyruvate, 2; Na-lactate, 2; glucose, 10; and HEPES, 10; the pH was adjusted to 7.4 with NaOH, and the saline was supplemented with vitamins (1:100) and amino acids (1:100) formulated for Eagles minimum essential medium ...
p,With a previous observation of the defensive response against elevated temperatures and nutrient variation in ,em,Escherichia coli ,/em,in different culture media, present study further demonstrated the consequences of high temperature on the growth of ,em,Pseudomonas ,/em,spp. (SUBP01) ,em,Bacillus ,/em,spp. (SUBB01), and ,em,Salmonella ,/em,spp. (SUBS01). Bacterial growth was measured through the enumeration of the viable and culturable cells on Luria-Bertani (LB) and nutrient agar (NA) plates up to 72 hours, microscopic experiments were conducted to monitor subsequent physiological changes, and finally the loss of culturability due to high temperature stress was further confirmed by the observation of growth incapability through spot tests. A slight reduction in the culturable cells of ,em,Pseudomonas ,/em,spp. (SUBP01) was observed at 45°C after 24 to 72 hours of incubation in the LB media while no such inhibition of growth was noticed in the nutrient media. Notably, ,em,Bacillus ...
With Earles salts, L-glutamine, and 92 mg/L D-valine substituted for L-valine. Store at 2˚ to 8˚C.. Find this and many more products.
Externally supplied protein (bovine serum albumin, BSA) affects root development of Arabidopsis, increasing root biomass, root hair length, and root thickness. While these changes in root morphology may enhance access to soil microenvironments rich in organic matter, we show here that the presence of protein in the growth medium increases the plants resilience to the root pathogen Cylindrocladium sp.. ...
In order to optimize culture conditions of bovine pulmonary endothelial cells (CPAE), we have compared different culture media, supports and extracellular matrices. Cell biomass was estimated by protein assay using Lowrys method. Different constituents including albumin, hydrocortisone, insulin, transferrin, triiodotyronine, Epidermal Growth Factor (EGF) and Fibroblast Growth Factor were separately added to Basal Defined Medium (BDM). Among them, BDM supplemented with EGF (1 ng/ml), hydrocortisone (100 nM), insulin (1 mu g/ml), triiodotyronine (2 nM) and linoleic acid complexed to albumin (10 mu g/ml) also named synthetic BDM appeared to be the best serum-free nutritive medium and showed similar results as compared to DMEM supplemented with 10% Fetal Bovine Serum (FBS). Several supports have then been tested including tissue culture polystyrene (as a reference), teflon, polycarbonate or poly(ethylene terephthalate) track-etched membranes. Among them, cells cultivated on surface treated ...
Objectives: The composition of nuclear membrane proteins (NMP) of fibroblasts derived from the periodontium is unknown. Identification of NMP is complicated since extraction of membrane proteins can be difficult due to the lipophilic nature of these molecules. The purpose of this study was to compare the quantity of unique identifiable membrane proteins using a single enzymatic wash preparation versus a two wash preparation for digestion and denaturation in proteomic analysis. Methods: GF used were obtained from 2 human biopsies derived from non-inflamed tissue in the retromolar pad area. Cells were cultured and propogated in Eagles minimal essential medium supplemented with 10% fetal bovine serum. GF were harvested when confluent in the 4th cell culture passage. NMP were isolated by differential centrifugation. 15 μg of protein from each sample was digested with trypsin or trypsin followed by a urea wash, followed by iTRAQ™ mass labeling for peptide qualitative and quantitative ...
A method for matching a query to a portion of media, includes receiving a query relating to media of interest and searching, based upon the query, an index of annotations. Each of the annotations represents a respective item of available media and includes a plurality of annotation values. Each of the plurality of annotation values represents a portion of the represented item of available media. By matching the query to an annotation value within the index, the start time of a media stream forming the portion of the item of available media represented by the identified annotation value can be identified. The identified media stream start time can then be provided in response to the query, allowing the appropriate portion of the applicable item of available media to be directly accessed.
b,This study proposed a methodology to evaluate the potential of mid-infrared (MIR) spectroscopy as a process analytical technology (PAT) tool for in situ (in-line) monitoring of cell culture media constituents, paving the way for on-line bioprocess monitoring and control of mammalian cell cultures. The methodology included a limit of detection (LOD) analysis and external influence investigation in addition to the calibration model development. The LOD analysis in the initial step provided a detailed procedure by which to evaluate the monitoring potential of the instrument of choice, for the application in question. The external influence study highlighted the potential difficulties when applying this technique to a typical mammalian cell culture. A comparative investigation between a fixed conduit immersion probe and flexible fiber-optic immersion probe was also carried out. Limitations associated with the use of MIR spectroscopy in the cell culture environment were also examined. A preliminary ...
Gallery Server Pro is a complete, stable ASP.NET gallery for sharing photos, video, audio and other media. This article presents the overall architecture and major features.; Author: Roger Martin; Updated: 19 Oct 2013; Section: Content Management Server; Chapter: Enterprise Systems; Updated: 19 Oct 2013
Mass production: Mass production, application of the principles of specialization, division of labour, and standardization of parts to the manufacture of goods. Such manufacturing processes
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Abstract Cell culture media (RPMI 1640, Dulbeccos Minimal Essential Medium and yeast extract-peptone-glucose medium) were found to oxidize dichlorodihydrofluorescein diacetate and..
Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the Contacts provided below. For general information, see Learn About Clinical Studies. ...
Dehydrated tissue culture media, microbiology, microbiological media, culture media, microbiology selective, enriched media, agar preparation, microbiology types, plant tissue, microbiological medium, microbial media, media bacteria, bacterial media, microbiological culture, mixed culture microbiology, complex media microbiology, autoclave microbiology, plant tissue culture media
The multipotency and anti-inflammatory effects of mesenchymal stem cells (MSCs) make them attractive for cell therapy in regenerative medicine. A large number of MSCs is required for efficient therapy owing to the low homing efficiency of MSCs to target sites. Furthermore, owing to limitations in obtaining sufficient amounts of MSCs, in vitro expansion of MSCs that preserves their differentiation and proliferative potential is essential. The animal factor included in culture media also limits clinical application. In this study, adipose-derived MSCs showed a significantly higher proliferation rate in STK2, a chemically-defined medium, than in DMEM/FBS. The expression of MSC surface markers was increased in the culture using STK2 compared to that using DMEM/FBS. Tri-lineage differentiation analyses showed that MSCs cultured in STK2 were superior to those cultured in DMEM/FBS. In addition, MSCs cultured in STK2 showed a reduced senescence rate, small and homogenous cell size, and were more genetically
Unless specified otherwise, MP Biomedicals products are for laboratory research use only, not for human or clinical use. For more information, please contact our customer service department ...
Buy the Two Little Fishies CDX Carbon Dioxide Adsorption Media System - 750 ml for your aquarium and read product reviews, watch videos and see detailed specs at MarineDepot.com.
... E. coli on Nutrient agar, Blood agar, MacConkey agar, EMB agar, m-ENDO agar, MHA, VRBA, CLED agar and Liquid media.
Comparing Media Systems Beyond the Western World by Daniel C. Hallin, 9781139202794, available at Book Depository with free delivery worldwide.
Deivanayaki M, Antony Iruthayaraj P Nehru Arts and Science College, Coimbatore - 641 105, Tamilnadu, India Department of Biotechnology, Bharathiar University, Coimbatore - 641 046, Tamilnadu, India Key words: Culture media, media from local raw materials, conventional media, media formulation. Abstract The high cost of the microbial culture media paved a way for the production…
The most common growth media for microorganisms are nutrient broths (liquid nutrient medium) or Luria Bertani medium (LB medium or Lysogeny Broth). Liquid media are often mixed with agar and poured into petri dishes to solidify. These agar plates provide a solid medium on which microbes may be cultured. They remain solid, as very few bacteria are able to decompose agar. Bacteria grown in liquid cultures often form colloidal suspensions.. The differences between growth media used for cell culture and those used for microbiological culture are because cells derived from whole organisms and grown in culture often cannot grow without the addition of, for instance, hormones or growth factors which usually occur in vivo.[4] In the case of animal cells, this difficulty is often addressed by the addition of blood serum to the medium. In the case of microorganisms, there are no such limitations, as they are often unicellular organisms. One other major difference is that animal cells in culture are often ...
Differential media are especially helpful when trying to identify a specific species. Bacteria can show widely different characteristics when grown in contact with various nutrients. A blood plate, one type of differential media, is often used to identify whether bacteria can digest blood cells. This is especially useful when diagnosing infection diseases. Streptococcus pneumoniae, a species that causes one type of pneumonia, can be identified partly thanks to its hemolytic activity, hemolysis being the breakdown of red blood cells. There are three different characteristic types of hemolysis that can be seen by growing bacteria on blood agar: alpha, beta, and gamma. Alpha hemolytic bacteria will only affect the medium that the colony is touching, turning the agar from red to green. Beta hemolytic bacteria cause a rather more dramatic change, removing the color from the media for some distance around each colony. Alpha hemolysis is only a partial digestion of the blood cells, while beta is a ...
Differential media are especially helpful when trying to identify a specific species. Bacteria can show widely different characteristics when grown in contact with various nutrients. A blood plate, one type of differential media, is often used to identify whether bacteria can digest blood cells. This is especially useful when diagnosing infection diseases. Streptococcus pneumoniae, a species that causes one type of pneumonia, can be identified partly thanks to its hemolytic activity, hemolysis being the breakdown of red blood cells. There are three different characteristic types of hemolysis that can be seen by growing bacteria on blood agar: alpha, beta, and gamma. Alpha hemolytic bacteria will only affect the medium that the colony is touching, turning the agar from red to green. Beta hemolytic bacteria cause a rather more dramatic change, removing the color from the media for some distance around each colony. Alpha hemolysis is only a partial digestion of the blood cells, while beta is a ...
Internet-Draft Media Type Registration June 2011 Note that this syntax is somewhat more restrictive than what is allowed by the ABNF in [RFC2045] and amended by [RFC2231]. There is no defined syntax for parameter values. Therefore registrations MUST specify parameter value syntax. Additionally, some transports impose restrictions on parameter value syntax, so care should be taken to limit the use of potentially problematic syntaxes; e.g., pure binary valued parameters, while permitted in some protocols, probably should be avoided. New parameters SHOULD NOT be defined as a way to introduce new functionality in types registered in the standards tree, although new parameters MAY be added to convey additional information that does not otherwise change existing functionality. An example of this would be a "revision" parameter to indicate a revision level of an external specification such as JPEG. Similar behavior is encouraged for media types registered in the vendor or personal trees but is not ...
You are here because you want to bulk up, you want to lean out, or you want to get ripped. Either way, youre in luck and can learn from my mistakes while looking for the best muscle growth supplement. My Issue Finding the Best Muscle Growth Su ...
Every year, the Department of Water Resources issues technical bulletins, reports, and other publications and media documenting the measurement, protection, use, and management of Californias water resources. This DWR Publications Repository is a partial and growing list of the publications and other media produced and distributed by the Department. In this list you find a variety of types of publications and other media ranging from print material, to audio and video files. If the publication or other media is available in electronic format, a link will point to the appropriate electronic file. In many cases, publications are only available in hardcopy and cannot be downloaded. A contact phone number and email will be provided for cases where an electronic download is not available. Please use the Comments or Suggestions link in the footer to request an accessible version of text-based documents. If you are searching for a particular publication or media item, and cannot find it, for more ...
Every year, the Department of Water Resources issues technical bulletins, reports, and other publications and media documenting the measurement, protection, use, and management of Californias water resources. This DWR Publications Repository is a partial and growing list of the publications and other media produced and distributed by the Department. In this list you find a variety of types of publications and other media ranging from print material, to audio and video files. If the publication or other media is available in electronic format, a link will point to the appropriate electronic file. In many cases, publications are only available in hardcopy and cannot be downloaded. A contact phone number and email will be provided for cases where an electronic download is not available. Please use the Comments or Suggestions link in the footer to request an accessible version of text-based documents. If you are searching for a particular publication or media item, and cannot find it, for more ...
Cell culture medium. A precision micropipette is used to transfer cell culture medium (Dulbeccos Modified Eagles medium, DMEM) to a multi-well plate. Cell culture medium (or growth medium) is commonly used in biological research to maintain and grow cells in tissue culture. Studio shot. - Stock Image C028/0709
image size 12KB). Fermentation. A vial is removed from liquid nitrogen storage and the preserved microorganisms are streaked on an agar plate. The isolated colonies are individually transferred to selective agar media for verification of genotype and phenotype. Colonies which are positive on the microbiological tests and are strongly resistant to the antibiotic (s) are selected as the starting inoculum. Depending on the process, several stages with varying medium composition and increasing volume are used before the inoculum reaches sufficient quantity. The final inoculum, from several hundred liters and up, is usually 5 to 15% by volume of the production fermenter.. Chemically defined media are normally used in the inoculum preparation, second stage (s) and production fermentation, because the recombinant microorganisms usually have stringent nutritional requirements. Traces of antibiotics are also added to the medium to help prevent the mutant from taking over the slower growing recombinant ...
AthenaES s proprietary media formulations dramatically increase the expression of recombinant proteins over traditional blends. Use our Media Optimization Kit to test for the optimum media for your unique protein.
A biological culture growth and observation system provides for the growth of a biological sample in a growth medium, and the observation of the grown sample (organism) through a microscope objective once growth is sufficient as to permit discrimination of the biological sample. Observation and growth is achieved in a single device. The system includes a tray with a top, and a sealable section on the top. Included within the tray is a depression, or well, that receives the growth medium to grow the biological organism in the well. A lid is sealable and resealable around the sealable section on the top of the tray. An interior side of the lid, that faces the growth medium, does not fog up in the presence of the growth medium. Optionally included is a barrier layer that is positioned over the well. The barrier layer minimizes evaporation from the growth medium and permits the biological culture growth and observation system to have a long shelf life.
In the present study, we aimed to preincubate MCF-10A cells with pioglitazone and/or serum-rich growth media and to determine adhesive and non-adhesive interactions of the preincubated MCF-10A cells with BT-474 cells. For this purpose, the MCF-10A cells were preincubated with pioglitazone and/or serum-rich growth media, at appropriate concentrations, for 1 week. The MCF-10A cells preincubated with pioglitazone and/or serum-rich growth media were then co-cultured adhesively and non-adhesively with BT-474 cells for another week. Co-culture of BT-474 cells with the preincubated MCF-10A cells, both adhesively and non-adhesively, reduced the growth of the cancer cells. The inhibitory effect of the preincubated MCF-10A cells against the growth of BT-474 cells was likely produced by increasing levels of soluble factors secreted by the preincubated MCF-10A cells into the conditioned medium, as immunoassayed by ELISA. However, only an elevated level of a soluble factor distinguished the conditioned medium
Serum-Free media for cell cultures from Biological Industries include chemically-defined, protein-free, and serum-free media formulations for mammalian and stem cell cultures.
Cells that are grown in the Petri dish are indispensable tools in biomedical research. From cancer research to neurosciences, from immunology to virology: In all conceivable disciplines, scientists use cell cultures to examine, for example, the effects of new drugs, responses to signaling molecules, or the infectious behavior of pathogens. However, it is quite common that the cells that grow in the red culture medium are not what the researchers believe them to be. In the hustle of laboratory routine, it happens that tiny drops of culture medium are accidentally transferred from one container to another. If rapidly growing cells are introduced into other culture dishes in this way, they may swiftly outnumber other cells. Another critical issue is the permanent use of genetically variable cancer cells in laboratory experiments. These cells evolve over time by continuous mutations and after a while they no longer correspond to the initial cells. Frequently, however, the problem is simply caused by ...
books.google.ithttps://books.google.it/books/about/Continuous_Cultivation_of_Microorganisms.html?hl=it&id=7Yw_AAAAYAAJ&utm_source=gb-gplus-shareContinuous Cultivation of Microorganisms ...
Does anyone know if slip caps (instead of screw caps) are ok to use with media such as TSA, TSI, and other agar slants? Are there problems with contamination or water loss after they are a month or two old? Any information would be appreciated. Thanks Jamie ...
... International supplier of animal free productivity enhancing cell ...Fort Collins CO (PRWEB) December 18 2008 -- The 2008 Cell Engineerin...Over the course of the three day conference in Santa Barbara CA InV...InVitrias Director of Cell Culture Dr. Steve Pettit presented at t...,InVitria's,Cell,Culture,Media,Components,Showcased,at,WilBio's,2008,Cell,Engineering,Meeting,,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
Medium development beyond production media: Chemically defined media for transfection and single cell cultivation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Toshiba Corporations (TOKYO:6502) Semiconductor & Storage Products Company today announced the mass production start of TB9042FTG, a system regulator IC with monitoring function for the motor control system of electric and hybrid vehicles. The new IC is optimized for high efficiency systems, with a strengthened monitoring function to reduce potential risks that threaten the security of the system. This Smart News Release features multimedia. View the full release here: http://www.businesswire.com/news/home/20151015005618/en/ Toshiba: TB9042FTG, a system regulator IC with monitoring function for the motor control system of electric and hybrid vehicles. (Photo: Business Wire) Failure of the system regulator IC leads to malfunction of electronic devices. The system particularly loses functionality when microcontroller (MCU) failure impacts on operation.
We are proud to announce that we have signed Seattle based Sounds of Mass Production (a.k.a. SMP). Once described as Cyber-Punk, SMP are an industrial rock act with heavy influences in rap and punk. Its danceable, aggressive, and diverse. Next year, SMP will make their WTII debut with the release of a (to be titled) full length album. For more information visit the WTII website.
We are proud to announce that we have signed Seattle based Sounds of Mass Production (a.k.a. SMP). Once described as Cyber-Punk, SMP are an industrial rock act with heavy influences in rap and punk. Its danceable, aggressive, and diverse. Next year, SMP will make their WTII debut with the release of a (to be titled) full length album. For more information visit the WTII website.
In a home audio visual network including a plurality of devices coupled via an IEEE 1394 bus, a system for accessing a media drive mechanism of a multi-item-type media player. The multi-item-type media player can play any type of disc media item. A media drive mechanism is included within the multi-item-type media player and is configured to play or record the media item stored within the multi-item-type media player. A computer system is built-in to the multi-item-type media player. A software based media player model executes on the computer system, and in turn, causes the computer system to implement a method of accessing the media drive mechanism. In so doing, the computer system interfaces with a plurality of devices coupled to the multi-item media player via an IEEE 1394 communications link of an IEEE 1394 based network and provides a standardized command set for the media drive mechanism. The media player model provides a set of standardized commands that allow the plurality of devices on the
Donor Horse Serum (DHS) is derived from healthy, live animals greater than 12 months of age. The donor animals receive regular veterinary inspection and care, and are kept in carefully managed and strictly segregated herds. The donor animals are all gelded draft horses in order to limit lot-to-lot hormone level variations A variety of cell types can be successfully propagated in DHS. It is frequently used in combination with bovine sera or independently to supplement media for mammalian cell culture. DHS is often utilized in bovine virus research applications since it does not contain bovine antibodies. It is also used as a supplement in some bacteriological media. Donor Horse Serum, Heat Inactivated is treated by heat inactivation where serum is exposed to a temperature of 56 °C for 30 minutes under controlled conditions. Learn more.
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Hanna Rose Shell, a historian of science and technology, and a media maker, is an Associate Professor of Science, Technology and Society at MIT, and an affiliate in Comparative Media Studies. Shell will give a free talk titled, "Media Fabric: A History, Theory, and Practice of Textile Reuse.". Shell received a Ph.D. in the History of Science (Harvard, 2007) and an M.A. in American Studies (Yale University, 2002). In both her text and film-based scholarship, Shell studies the production, use, and transformation of often marginalized artifacts, located at the interstices of the found and the fabricated. Her current book project is a historical and theoretical investigation into the epistemology of reuse, in which objects of analysis include old clothes, decomposing vegetable matter, and other artifacts of strategic repurposing. Works from the last year include the scholarly article and media essay "Cinehistory and Experiments on Film," published in Journal of Visual Culture (Dec 2012) and the book ...
Media objects are transformed into active, connected objects via identifiers embedded into them or their containers. In the context of a users playback experience, a decoding process extracts the identifier from a media object and possibly additional context information and forwards it to a server. The server, in turn, maps the identifier to an action, such as returning metadata, re-directing the request to one or more other servers, requesting information from another server to identify the media object, etc. The linking process applies to broadcast objects as well as objects transmitted over networks in streaming and compressed file formats.
Put our media analytics expertise to work for you. To help you increase yield, simplify feeding regimes, and optimize production economics, we analyze the major nutritional components in your spent media samples. Experienced in media optimization, process development, and analytical testing, Gibco BioProduction Services scientists generate data on validated instrumentation using optimized protocols for cell culture media.
floating THP-1 cells - medium change advice - posted in Tissue and Cell Culture: Hello, I was adamantly asked to culture THP-1 cells and did so, by thawing a cryovial and seeding the cells in a 25 cm flask. 3 days later 99% of cells are floating, which I reckon is OK. Isnt it? So, do I need to change the medium before they reach ~8x 10^5 cells/ml and are ready to be passed? Since these are floating cells, how do I do that? Also, would a 1/3 split ratio be OK for these cells? Th...
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In the creative brief, right under the media plan summary, there should be a description of each media property and the particularities of its audience. Which means theres a job for someone to do nothing but watch TV, read magazines, browse the web, and take lots of notes, sort of a mix between an account and media planners. After all, we have well-paid professionals hunting down and explaining obscure cultural phenomena - why not somebody who keeps track of the stuff thats popular today. Somebody whod work in the Chief Culture Officers department ...
Several laboratories had tested bioactivity of the materials in commercially available solution DMEM (Dulbeccos Modified Eagles Medium) that is normally used for cultivation of cell cultures. The objective of this work was to find out whether it is possible to replace TRIS-buffered SBF currently used for b
I want to investigate the effect of conditioned media on the behaviour of adherent cells. I need to grow my cells in high-serum media (10% FBS) for 5 days and then I need to add serum-free media to the same dish for 3 days. The idea is that I am trying to create conditioned media by letting the cells secrete different factors in the serum free media for 3 days. However, as I mentioned, before adding the serum-free media the cells were grown for 5 days in 10% FBS media. I do 3 long washes with PBS before changing from 10% FBS to serum-free media, but I am still worried that some serum proteins will bind to the dish/plasma membrane of the cells and will then be released into my serum-free media ...
...Media can register now for Europes premier cancer congress: The 2011 ...The 2011 European Multidisciplinary Cancer Congress will take place at...The congress will build on the success of the previous ECCO (European ...Leading experts in different cancer fields have developed a wide-rangi...,European,Multidisciplinary,Cancer,Congress,2011,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
Over a two week period, eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not all of these tests were performed on every culture, as some were used only for gram positive or gram negative bacteria. The tests performed and what constituted a positive or negative test are as follows: Lab day 1; today in lab we obtained the unknown mixed culture "041"and one brain-heart infusion agar (BHIA). The first step was the preparation of the medium, the bottom of the BHIA dish was labeled with the bacterium number, initials, and section; then divided into four quadrants. The second step, we used the septic technique to transfer a small amount of culture with a flame-sterilized inoculating loop to the first quadrant, flamed and cooled the loop again then transferred a small amount of the culture from the first quadrant to the second using the quadrant streaking method as illustrated on page 18 of the lab manual, repeating this process until all four quadrants ...
Many themes and images are ripe with multiple interpretations, narratives, and meanings. In these series I begin with a base screen printed image which is elaborated upon with other media. Each piece within the series tells a different chapter in a story or a different stage in an equation. However, together the work speaks loudest, the whole being more than the sum of its parts. various media
Little Poetic Interactions consists of simple micro-controllers in charge of interpreting the impulses controlled by the analog actuators to later translate it into keystrokes which are efficiently interpreted by the computer actuating the poem sequences programmed in Flash.
The application of fluorescence excitation-emission matrix (EEM) spectroscopy to the quantitative analysis of complex, aqueous solutions of cell culture media components was investigated. These components, yeastolate ...
This is the latest in a series of papers evaluating the efficacy of specific inositol stereoisomers to ameliorate phenotypes of Aβ pathology. It extends investigation of effects of Aβ oligomer-containing 7PA2 cell culture media on synaptic function into behavioral tasks in a rat infusion model. The observed effects appear to be due to blocking oligomers binding to neuronal membranes, which would directly affect toxicity while also improving the chances for clearance of small oligomers.. Importantly, the investigators measure and report dose-dependent levels of AZD-103 in CSF of rats who have had either 30, 100, or 300 mg/kg/day of AZD-103 added to their drinking water. The concentrations attained are 20-50 microM, well above the single-digit microM required to neutralize the effects of the infused 7PA2 cell media. This direct demonstration of AZD-103 bioavailability is often a difficult hurdle to overcome for putative therapeutics. Of course, the pharmacodynamics and pharmacokinetics remain to ...
You are currently viewing games for Amiga that are marked as Media Type : 3.5" Floppy Disk. Games for other platforms are also tracked by this attribute. You can restrict the list of games below to any of the following platforms ...
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Unparalleled performance - tested for optimal growth SUMMARY. CRITERION™ HardyCHROM™ Candida is a selective and differential medium containing chromogenic substrates. After degradation by specific enzymes, the substrates release different colored compounds. Certain species or groups of organisms can then be differentiated with a minimum number of confirmatory tests. Colonies of C. albicans appear green to dark metallic green, C. tropicalis colonies appear medium blue to dark metallic blue with a blue halo, and C. krusei colonies appear flat, often rough or crenated, and pink to medium pink in color. Other species appear pink, often with a darker mauve center (C. glabrata and other species). Other yeasts may appear white to pink. Additionally, HardyCHROM™ Candida can be used in conjunction with Rapid Trehalose Broth (Cat. no. Z205) or GlabrataQuick™ (Cat. no. Z298) to aid in the identification of C. glabrata. When HardyCHROM™ Candida is used as the primary plating medium, only colonies ...
Although there is ample interrogation of advertising/commercial/media culture in critical pedagogy, there is little attention paid to the fine arts and to aesthetic experience. This lacuna is all the
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|p|Bacterial culture media are routinely used in microbiology lab to cultivate bacteria. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. These products are suitable for non-selective cultivation of bacterial strains for cloning, DNA plasmid production and production of recombinant proteins. Also, these can be suitable for selective cultivation when appropriate antibiotics are added. To review all biochemicals offered on this website check the main categories under Life Sciences which include both Affymetrix/USB and Alfa Aesar product options.|/p|
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Freschi, Carla Roberta, Carvalho, Luiz Fernando de Oliveira e Silva and Oliveira, Celso Jos Bruno de Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR). Braz. J. Microbiol., Dec 2005, vol.36, no.4, p.363-367. ISSN 1517- ...
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AthenaES s proprietary media formulations dramatically increase the expression of recombinant proteins over traditional blends. Use our Media Optimization Kit to test for the optimum media for your unique protein.
Protocols: The hMSCs (Neuromics, Edina, MN) used in experiments were at passage 3-9. Each passage of hMSCs was maintained on the TCPS dishes with a pre-coating of Geltrex reduced growth factor basement membrane matrix (Invitrogen, CIBCO, NY). All hMSCs were maintained in the growth medium, Dulbeccos modified Eagles medium-low glucose (DMEM-LG) supplemented with mesenchymal cell growth supplement (MSCGM, Lonza) containing L-glutamine, penicillin, and streptomycin, and incubated in an atmosphere containing 5% CO2 at 37 °C. The medium was replenished every 3 to 4 days. For osteogenic differentiation, the hMSCs were cultured in the osteogenesis induction medium, DMEM-LG supplemented with mesenchymal stem cell osteogenesis kit (Chemicon, Cat. No. SCR028), and incubated on the TCPS dishes (control) and test devices. To study the drug release from the devices, hMSCs were cultured in the osteogenesis induction medium in the absence of DEX. The fresh medium was replaced every 2 to 3 days ...
Protocols: The hMSCs (Neuromics, Edina, MN) used in experiments were at passage 3-9. Each passage of hMSCs was maintained on the TCPS dishes with a pre-coating of Geltrex reduced growth factor basement membrane matrix (Invitrogen, CIBCO, NY). All hMSCs were maintained in the growth medium, Dulbeccos modified Eagles medium-low glucose (DMEM-LG) supplemented with mesenchymal cell growth supplement (MSCGM, Lonza) containing L-glutamine, penicillin, and streptomycin, and incubated in an atmosphere containing 5% CO2 at 37 °C. The medium was replenished every 3 to 4 days. For osteogenic differentiation, the hMSCs were cultured in the osteogenesis induction medium, DMEM-LG supplemented with mesenchymal stem cell osteogenesis kit (Chemicon, Cat. No. SCR028), and incubated on the TCPS dishes (control) and test devices. To study the drug release from the devices, hMSCs were cultured in the osteogenesis induction medium in the absence of DEX. The fresh medium was replaced every 2 to 3 days ...
Readers of such reviews seek both an overview of the product and an assessment of its quality relative to similar works. Thus, these reviews usually include description and opinion, both of which may extend to broader issues raised by the work. There is often considerable room for individual style and expression in these critiques, but supporting evidence for the reviewers praise or criticism is essential. |
Readers of such reviews seek both an overview of the product and an assessment of its quality relative to similar works. Thus, these reviews usually include description and opinion, both of which may extend to broader issues raised by the work. There is often considerable room for individual style and expression in these critiques, but supporting evidence for the reviewers praise or criticism is essential. |
54 Batch Culture Continuous Culture cells are grown in a fixed volume of liquid medium in a closed vessel nutrients are added and cells harvested at a constant rate No microorganisms, fluid or nutrients are added or removed from the culture during the incubation period Volume of suspension is kept constant Used for producing secondary metabolites, such as penicillin and other antibiotics, which are relatively unstable and not essential for the growth of the culture Fermenter does not have to be emptied, cleaned and refilled very often Secondary metabolites can be extracted economically only when they reach a high concentration in the culture Production is almost continuous Continuous cultivation needs sophisticated equipment to maintain constant conditions. Highly trained staff need to operate the equipment. Therefore this process can be expensive ...
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In this article,study fermentation conditions of the recombinant strains in shake culture,aiming at improving the protein expression and fermenting in 5L fermentation tank. The result indicates that the suitable fermentation conditions for the recombinant cell are as below: the temperature of cell growth and protein expression is 30℃,the methanol feeding is 2%(volume ratio of the medium)per day,the initial pH is 6.2,the induction time is 72h.According to these conditions,the enzyme activity of shake culture is increased by 64% comparing with before optimization. In 5L fermentation cultivate,the enzyme activity reaches 40864U·mL-1,86 times of the shake culture. ...
AIMS--To determine the best medium for the primary isolation of Helicobacter pylori. METHODS--Sixty six gastric mucosal biopsy specimens frozen in 1 ml Cysteine Albimi media with 20% glycerol from 22 histologically proven H pylori infected patients were cultured on brain heart infusion agar (BHIA) with 7% fresh whole defibrinated horse blood, egg yolk agar (EYA), Columbia blood agar-cyclodextrin agar (CBA-Cd), and commercial trypticase soy agar (TSA) supplemented with 5% sheep blood. RESULTS--Successful primary isolation of H pylori was 96% with BHIA, 78% with TSA, 64% for EYA, and 32% with CBA-Cd. Colonies appeared earlier on BHIA (4.7 +/- 0.1 days, 5.3 +/- 0.4 days, 5.3 +/- 0.4 days, and 7.1 +/- 0.9 days for BHIA, TSA, EYA, and CBA-Cd) and there were more colonies on BHIA than on CBA-Cd, EYA or TSA (599 +/- 88, 104 +/- 66, 260 +/- 107, and 358 +/- 89, respectively). CONCLUSIONS--Success of a medium for passage of isolates apparently does not reliably predict usefulness for primary isolation. ...
0060] The term "cell proliferation control" means a procedure in which cell proliferative capacity is stopped at a desired time, the cell concentration is maintained without significant damage affecting the viability of cells to the cells, and subsequently cell proliferation is restarted at a desired time. In other words, in a method for controlling cell proliferation of the present invention, the proliferation of primate pluripotent stem cells can be inhibited with a maintenance medium for pluripotent stem cells of the present invention, and after a desired period elapsed, the proliferation of primate pluripotent stem cells can be promoted with a culture medium containing glucose. In a method for controlling cell proliferation of the present invention, the growth rate and the morphology of pluripotent stem cells following the procedure in which the proliferation is inhibited with a maintenance medium for pluripotent stem cells of the present invention, and after an inhibition period passed, the ...
Links connect you with a Word document list of the media placements.. NAMI Mass Media Placements - June 2016. NAMI Mass Media Placements - May 2016. NAMI Mass Media Placements - April 2016. NAMI Mass Media Placements - March 2016. NAMI Mass Media Placements - February 2016. NAMI Mass Media Placements - January 2016. NAMI Mass Media Placements - December 2015. NAMI Mass Media Placements - November 2015. NAMI Mass Media Placements - October 2015. NAMI Mass Media Placements - September 2015. NAMI Mass Media Placements - July-August 2015. NAMI Mass Media Placements - June 2015. NAMI Mass Media Placements - May 2015. NAMI Mass Media Placements - April 2015. NAMI Mass Media Placements - March 2015. NAMI Mass Media Placements - February 2015. NAMI Mass Media Placements - January 2015. NAMI Mass Media Placements - December 2014. NAMI Mass Media Placements - November 2014. NAMI Mass Media Placements - October 2014. NAMI Mass Media Placements - August 2014. NAMI Mass Media Placements - July 2014. NAMI ...
Driven by the increase in industrialization and population, the global demand of energy and material products is steadily growing. Microalgae have come into prominence in the past several decades due to their ability to utilize solar energy to fix atmospheric carbon dioxide, and produce biomass and lipids at productivities much higher than those possible with terrestrial biomass. The main objective of this research is to maximize the biomass and lipid production of Chlorella vulgaris by varying different external conditions so as to achieve the ideal feedstock for the production of commodity chemicals and implement wastewater treatment. The effects of various culture medium compositions on Chlorella vulgaris growth and lipid production were investigated using batch culture. Thirteen culture media: Modified Chus No. 10, Bold basal, BG-11, Modified BG-11, N-8, M-8, RM, Modified Spirulina, F-si, Foggs Nitrogen free, Fog, F/2, and Johnson medium were compared in terms of optical density, biomass
TY - JOUR. T1 - Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares. AU - Flayhart, Diane. AU - Hindler, Janet F.. AU - Bruckner, David A.. AU - Hall, Geraldine. AU - Shrestha, Rabin K.. AU - Vogel, Sherilynn A.. AU - Richter, Sandra S.. AU - Howard, Wanita. AU - Walther, Rhonda. AU - Carroll, Karen C. PY - 2005/11. Y1 - 2005/11. N2 - Active surveillance for methicillin-resistant Staphylococcus aureus (MRSA) is among the strategies recommended by the Society for Healthcare Epidemiology of America for control of nosocomial MRSA infections. Infection control and laboratory personnel desire rapid, sensitive, and inexpensive methods to enhance surveillance activities. A multicenter study was performed to evaluate a new selective and differential chromogenic medium, BBL CHROMagar MRSA (C-MRSA) medium (BD Diagnostics, Sparks, MD), which enables recovery and concomitant identification of ...
Bbl sabouraud dextrose agar bbl sabouraud dextrose agar bd. Sabouraud dextrose agar (agar dextrosa sabouraud) licuar sabouraud dextrose agar deeps en tubos a mediante ebullición en baño maría.* candida albicans.. medicine for yeast contamination facet results candida. I requerir un experto en este resolver mi problema. Medicinal drug for yeast contamination aspect effects garlic will help due to candida albicans are usually.. Que significa candidato a magister candida albicans en. Fermenti lattici consistent with candida quali; candida albicans sintomas how long does it take to kill the candida on average? Que significa candidato a magister these.. Candida albicans en agar dextrosa sabouraud. Candida albicans en agar dextrosa sabouraud if you have a susceptible immune device, remedy might be greater hard. Candida albicans en agar dextrosa sabouraud the.. Sabouraud agar wikipedia. Candida albicans in sabouraud agar. Trichophyton terrestre in sabouraud agar. Sabouraud agar normally includes 40 ...
Abstract: Activities of prekallikrein and Hageman factor (factor XII) were studied in some natural and artificial infusion media: polyglukine, rheoglumane, gemodes, polypher, gelatinol, saline; in solutions for parenteral nutrition--hydrolysate of casein, aminopeptide, aminokrovine, glucose solutions as well as in 10% NaCl and novocain. All the solutions studied did not affect the purified preparations of prekallikrein, but some of them activated partially purified preparations of Hageman factor. However, all these solutions, except of Ringer solution, activated Hageman factor and prekallikrein in human blood serum. Activation of prekallikrein appears to occur via the active form of Hageman factor. Among the solutions studied casein hydrolysate, 5% glucose, gelatinol and novocain exhibited the highest stimulating effect on Hageman factor and prekallikrein. Possible mechanisms of the activation are discussed ...
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria. Ammonium Sulfate Monopotassium phosphate Disodium phosphate Sodium Citrate Magnesium Sulfate sodium Zinc Sulfate Copper Sulfate L-Glutamic Acid (sodium salt) Ferric Ammonium Citrate Pyridoxine Hydrochloride Biotin Malachite Green Agar Cultures should be read within 5-7 days after inoculation and once a week thereafter for up to 8 weeks. Lowenstein-Jensen medium Middlebrook 7H9 Broth Atlas, Ronald M.; James W. Snyder (2006). Handbook of media for clinical microbiology. CRC Press. p. 307. ISBN 978-0-8493-3795-6. Middlebrook 7H10 Agar http://www.bd.com/ds/productCenter/295964. ...

Communicating Your Culture on Social Media | Central Illinois Business MagazineCommunicating Your Culture on Social Media | Central Illinois Business Magazine

This can feel risky because social media is so visible. Social media is often the first thing to be criticized in an ... Thats where social media is different from old marketing, and that might be challenging for some people within your ... one of the reasons for that success is because weve made an intentional effort to translate our culture to our social media ... Regardless if youre a rookie, or a veteran to social media, it seems to be one of those marketing tasks that can become ...
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Lets stand together and speak out about issues in our media and culture. Support causes that matter to you. Sign the hottest ... culture, or society? Its time to stand together in social activism and speak out about the issues in our media and culture ... Its up to us to hold our media and culture to the highest possible standards. Speaking up and getting involved in social ... By signing these petitions, or creating one of your own, you can affect policy, media, and culture. With your support of the ...
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Culture MediaCulture Media

Comedy womedy culture media, Microbiology , CULTURE MEDIA , PART 1, Culture medium, Media Prep, Microbial Culture Media - ... what is Culture media ,, different classification of culture media ,, Microbiology, ... Classification of Culture media 4.Type of culture media---Solid media , Liquid media, semisolid media 5.Enriched media, 6. ... Classification of Culture media 4.Type of culture media---Solid media , Liquid media, semisolid media 5.Enriched media, 6. ...
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Labware, Culture Media, Yeast MediaLabware, Culture Media, Yeast Media

Cookies are important for the proper functioning of a website. In order to improve your experience, we use cookies to keep the login information and provide a secure connection, collect the statistics to optimize the functionality of the website and adapt the content to your interests. Click on "Accept and Continue" to accept cookies and access to the website or you can refer to our Privacy Policy.. ...
more infohttps://www.mpbio.com/life-sciences/lab-instrumentation/labware/media_category_slr:culture_media/media_type_slr:yeast_media

Culture MediaCulture Media

... from the most comprehensive global news network on the internet. International News and analysis on current ... Culture Asia. Culture Review. Deutsche Media. Espana Media. E.Telemedia. Harlem Culture. Harlem Life. Islam Generation. Islam ... Culture. Fashion. Film. Hollywood. Bollywood. Media. Music. Theatre. WN Broadcast. WN Arts & Music. WN Kids. WN Gaming. ... Ministere Culture. Multi Culturalist. Pop Idol. Religion Review. Religious Globe. World Media. ...
more infohttp://archive.wn.com/2005/12/21/1400/p/d7/c08b583a627c80.html

Ethylene Oxide Sterilization of Tissue Culture Media | ScienceEthylene Oxide Sterilization of Tissue Culture Media | Science

Liquid ethylene oxide, which kills bacteria, yeasts, and molds, was added to a tissue culture medium instead of the usual ... With some limitations, the use of ethylene oxide sterilization of tissue culture medium has been shown feasible. Data indicate ... in experiments involving the sterilization of test chemicals and components of the medium used for mammalian cells in culture. ...
more infohttp://science.sciencemag.org/content/142/3600/1654

Excitotoxicity Triggered by Neurobasal Culture MediumExcitotoxicity Triggered by Neurobasal Culture Medium

NMDA receptor agonist effects on development and survival should be considered when employing Neurobasal culture medium. ... Minimal Essential Medium (MEM), in contrast, produced no significant toxicity. Detectable Neurobasal-induced neuronal death ... Therefore, we were surprised that routine medium exchange with serum- and supplement-free Neurobasal killed as many as 50% of ... Neurobasal defined culture medium has been optimized for survival of rat embryonic hippocampal neurons and is now widely used ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0025633

Qilingshi Culture Media (Shanghai) Co., Ltd.: Private Company Information - BloombergQilingshi Culture Media (Shanghai) Co., Ltd.: Private Company Information - Bloomberg

Qilingshi Culture Media (Shanghai) Co., Ltd. company research & investing information. Find executives and the latest company ... Key Executives for Qilingshi Culture Media (Shanghai) Co., Ltd.. Qilingshi Culture Media (Shanghai) Co., Ltd. does not have any ... To contact Qilingshi Culture Media (Shanghai) Co., Ltd., please visit www.qilingshi.com.cn. Company data is provided by S&P ... Qilingshi Culture Media (Shanghai) Co., Ltd. engages in research, development and production of animation. The company was ...
more infohttps://www.bloomberg.com/research/stocks/private/snapshot.asp?privcapid=432051776

Prepared Culture Media | Thermo Fisher Scientific - RUPrepared Culture Media | Thermo Fisher Scientific - RU

Choose from our wide selection of ready-prepared media plates, bottles, tubes and special formats to meet your microbiology ... Reduce the burden of media fills with ready-to-use culture media in BioProcess containers with plug and play connectors for ... Thats why we bring together some of the most widely used, high-performing culture media formulations and put them on a single ... Isolate, identify, differentiate, and perform susceptibility testing on plated media from the experts in microbiological media ...
more infohttps://www.thermofisher.com/ru/en/home/industrial/microbiology/microbial-culture/prepared-culture-media.html

solid culture media microwave sterilizer Manufacturer‏solid culture media microwave sterilizer Manufacturer‏

... solid culture media microwave sterilizer Quality tunnel chemical powder dehumidifier/ dryer - find quality Chemical Material ... solid culture media microwave sterilizer is also suit for: molybdenum powder, ... microwave culture medium sterilizer 1.fast sterilization time 2.saving enerLD 3.cover less area. 4.excellent quality excellent ... solid culture media microwave sterilizer is used of electro-magnetic induction or infrared radiation to dry the raw material.By ...
more infohttp://www.evangelicaloutpost.com/cheap-49892763-solid-culture-media-microwave-sterilizer.html

Culture Media
     Summary Report | CureHunterCulture Media Summary Report | CureHunter

... such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been ... The variety of media that exist allow for the culturing of specific microorganisms and cell types, ... Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or ... such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been ...
more infohttp://www.curehunter.com/public/keywordSummaryD003470-Culture-Media.do

A New Culture Medium for the Gonococcus | The BMJA New Culture Medium for the Gonococcus | The BMJ

A New Culture Medium for the Gonococcus. Br Med J 1917; 1 doi: https://doi.org/10.1136/bmj.1.2948.869 (Published 30 June 1917) ...
more infohttp://www.bmj.com/content/1/2948/869
  • This can make it feel as though you have to go back to the drawing board, or as if your business can't keep up with the rapid pace that social media changes. (centralillinoisbusiness.com)
  • Available are long-living nutrient pad sets, media ampoules with an incorporated membrane filter and cellulose pad, agar media and liquid broth media in bottles and tubes as well as culture media in the form of absorbent pads and petri dishes - presterilised in plastic magazines. (sartorius.com)
  • Solid culture media is a mixture of agar and nutrients poured into Petri dishes . (wn.com)
  • The nutrient media comply with international regulations and recommendations and supersedes the handling of glass ampoules. (sartorius.com)
  • When delicate aerobic or anaerobic conditions are called for or when you're looking to isolate and study a specific reaction, a tube of one of the world's favorite brands of prepared media is right here ready for you. (thermofisher.com)
  • Corning™ RPMI 1640 Medium (Mod. (fishersci.com)
  • Corning™ Dulbecco's Modification of Eagle's Medium (DMEM) 1X1, Powder is hybridoma tested. (fishersci.com)
  • The Corning Hepatocyte Maintenance Medium is optimized to cultivate primary human hepatocytes, a well-established in vitro model system for studying preclinical drug-induced liver injury (DILI). (corning.com)
  • The Corning stemgro MSC Medium was designed for the maximum expansion of human mesenchymal stem cells (MSCs) derived from bone marrow, cord blood, or adipose tissue. (corning.com)
  • The media can enable lab researchers to save time, reduce costs, and improve performance when conducting highly specialized bioprocess assays for the development of therapeutics, vaccines, diagnostics, and pharmaceuticals. (corning.com)
  • Identify infections and antimicrobial susceptibility quickly and accurately to guide optimal patient care with powerful manual and automated technologies, and a comprehensive line of media. (thermofisher.com)
  • The average pay for a Laboratory Assistant Culture Media is $43,831 a year and $21 an hour in Maryland, United States. (erieri.com)
  • The average salary range for a Laboratory Assistant Culture Media is between $32,907 and $52,928 . (erieri.com)
  • On average, a Bachelor's Degree is the highest level of education for a Laboratory Assistant Culture Media. (erieri.com)
  • Assists in a laboratory by preparing culture media used to develop vaccines and toxoids or to conduct chemical, microscopic, and bacteriologic tests. (erieri.com)
  • Culture.Media also carries out complementary web activities for projects undertaken by the institute, including subpages, advertising and marketing campaigns as well as social media communication projects. (iam.pl)
  • It was established in 2011 and covers research on "how popular culture and general media influence individual, group, and system behavior. (wikipedia.org)
  • 15. A method according to claim 1, in which the cell culture medium is a semi-solid medium. (freepatentsonline.com)
  • 16. A method according to claim 15, in which the semi-solid medium comprises methylcellulose. (freepatentsonline.com)
  • solid culture media microwave sterilizeris used of electro-magnetic induction or infrared radiation to dry the raw material.By microwave enerLD permeation, the material inner moisture quickly been heated and gasified,forming a strong outward diffusion situation, to make the moisture extremely quickly spread to the surface. (evangelicaloutpost.com)
  • No other ready-to-use culture media assures such consistently high quality and reproducible results up to 24 months. (sartorius.com)
  • Reduce the burden of media fills with ready-to-use culture media in BioProcess containers with plug and play connectors for easy integration. (thermofisher.com)
  • It's time to stand together in social activism and speak out about the issues in our media and culture that affect us all. (thepetitionsite.com)
  • The Ninety-Nine is a comic book featuring, for the first time a team of superheroes based on Islamic culture and religion. (tufts.edu)
  • Viruses , for example, are obligate intracellular parasites and require a growth medium containing living cells. (wn.com)
  • When growing cells in the lab, we have to create these conditions using culture media. (wn.com)
  • When you support your favorite media outlets and cultural institutions, you help to ensure that we all enjoy access to culture and the arts. (thepetitionsite.com)
  • When importing soil or growing media from non-EU countries and exporting them to non-EU countries, the requirements set by plant health legislation and fertiliser product legislation must be observed. (evira.fi)