Cullin Proteins
Ubiquitins
Alternate choice of initiation codon produces a biologically active product of the von Hippel Lindau gene with tumor suppressor activity. (1/749)
The VHL tumor suppressor gene has previously been reported to encode a protein of 213 amino acid residues. Here we report the identification of a second major VHL gene product with an apparent molecular weight of 18 kD, pVHL18, which appears to arise from alternate translation initiation at a second AUG codon (codon 54) within the VHL open reading frame. In vitro and in vivo studies indicate that the internal codon in the VHL mRNA is necessary and sufficient for production of pVHL18. pVHL18 can bind to elongin B, elongin C, and Hs-CUL2. When reintroduced into renal carcinoma cells that lack a wild-type VHL allele, pVHL18 suppresses basal levels of VEGF expression, restores hypoxia-inducibility of VEGF expression, and inhibits tumor formation in nude mice. These data strongly support the existence of two distinct VHL gene products in VHL tumor suppression. (+info)Cyclin D1 and D3 associate with the SCF complex and are coordinately elevated in breast cancer. (2/749)
D-type cyclins are important cell cycle regulators that promote cellular proliferation in response to growth factors by inactivation of the retinoblastoma protein (Rb). Cyclin D1 has been shown to be overexpressed in several cancer types and to act as an oncogene in breast cancers. As D-type cyclins are rate limiting for progression into S phase, the level at which they accumulate must be carefully regulated. Several mechanisms leading to overexpression of cyclin D1 have been reported including amplification, translocation and stabilization of the mRNA. Here, we present data showing elevated cyclin D1 protein in breast cancer samples in the absence of elevated mRNA level. Further, we found that in these cases, cyclin D3 protein also accumulates and that the coordinate increase in cyclin D1 and D3 occurs in 15% (7/47) of breast cancers. In addition we show that blocking the activity of the 26S proteosome results in the accumulation of cyclin D1 and D3, that both D-type cyclins are ubiquitinated and associate with Cul-1, a component of the SCF ubiquitin ligase complex. Finally, we show that the coordinated elevation of cyclin D1 and D3 is also observed in the breast cell line MCF-7 and demonstrate that the degradation of cyclin D1 and D3 is deficient in this cell line. These results indicate that cyclin D1 and cyclin D3 share a common mechanism of degradation and we propose that the coordinate increase of D-type cyclins observed in primary breast cancers reflects a defect in their proteolysis. (+info)Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. (3/749)
The von Hippel-Lindau (VHL) tumor suppressor gene is mutated in most human kidney cancers. The VHL protein is part of a complex that includes Elongin B, Elongin C, and Cullin-2, proteins associated with transcriptional elongation and ubiquitination. Here it is shown that the endogenous VHL complex in rat liver also includes Rbx1, an evolutionarily conserved protein that contains a RING-H2 fingerlike motif and that interacts with Cullins. The yeast homolog of Rbx1 is a subunit and potent activator of the Cdc53-containing SCFCdc4 ubiquitin ligase required for ubiquitination of the cyclin-dependent kinase inhibitor Sic1 and for the G1 to S cell cycle transition. These findings provide a further link between VHL and the cellular ubiquitination machinery. (+info)Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1. (4/749)
Control of cyclin levels is critical for proper cell cycle regulation. In yeast, the stability of the G1 cyclin Cln1 is controlled by phosphorylation-dependent ubiquitination. Here it is shown that this reaction can be reconstituted in vitro with an SCF E3 ubiquitin ligase complex. Phosphorylated Cln1 was ubiquitinated by SCF (Skp1-Cdc53-F-box protein) complexes containing the F-box protein Grr1, Rbx1, and the E2 Cdc34. Rbx1 promotes association of Cdc34 with Cdc53 and stimulates Cdc34 auto-ubiquitination in the context of Cdc53 or SCF complexes. Rbx1, which is also a component of the von Hippel-Lindau tumor suppressor complex, may define a previously unrecognized class of E3-associated proteins. (+info)Recruitment of a ROC1-CUL1 ubiquitin ligase by Skp1 and HOS to catalyze the ubiquitination of I kappa B alpha. (5/749)
Activation of the transcription factor NF-kappa B in response to proinflammatory stimuli requires the phosphorylation-triggered and ubiquitin-dependent degradation of the NF-kappa B inhibitor, I kappa B alpha. Here, we show the in vitro reconstitution of the phosphorylation-dependent ubiquitination of I kappa B alpha with purified components. ROC1, a novel SCF-associated protein, is recruited by cullin 1 to form a quatemary SCFHOS-ROC1 holenzyme (with Skp1 and the beta-TRCP homolog HOS). SCFHOS-ROC1 binds IKK beta-phosphorylated I kappa B alpha and catalyzes its ubiquitination in the presence of ubiquitin, E1, and Cdc34. ROC1 plays a unique role in the ubiquitination reaction by heterodimerizing with cullin 1 to catalyze ubiquitin polymerization. (+info)ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. (6/749)
We have identified two highly conserved RING finger proteins, ROC1 and ROC2, that are homologous to APC11, a subunit of the anaphase-promoting complex. ROC1 and ROC2 commonly interact with all cullins while APC11 specifically interacts with APC2, a cullin-related APC subunit. YeastROC1 encodes an essential gene whose reduced expression resulted in multiple, elongated buds and accumulation of Sic1p and Cln2p. ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1- and E2-dependent manner. ROC1 mutations completely abolished their ligase activity without noticeable changes in associated proteins. Ubiquitination of phosphorylated I kappa B alpha can be catalyzed by the ROC1 immunocomplex in vitro. Hence, combinations of ROC/APC11 and cullin proteins proteins potentially constitute a wide variety of ubiquitin ligases. (+info)Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function. (7/749)
The von Hippel-Lindau tumor suppressor protein pVHL assembles with cullin-2 (hCUL-2) and elongin B/C forming a protein complex, CBCVHL, that resembles SKP1-CDC53-F-box protein ubiquitin ligases. Here, we show that hCUL-2 is modified by the conserved ubiquitin-like protein NEDD8 and that NEDD8-hCUL-2 conjugates are part of CBCVHL complexes in vivo. Remarkably, the formation of these conjugates is stimulated by the pVHL tumor suppressor. A tumorigenic pVHL variant, however, is essentially deficient in this activity. Thus, ligation of NEDD8 to hCUL-2 is linked to pVHL activity and may be important for pVHL tumor suppressor function. (+info)Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. (8/749)
SCFCdc4 (Skp1, Cdc53/cullin, F-box protein) defines a family of modular ubiquitin ligases (E3s) that regulate diverse processes including cell cycle, immune response, and development. Mass spectrometric analysis of proteins copurifying with Cdc53 identified the RING-H2 finger protein Hrt1 as a subunit of SCF. Hrt1 shows striking similarity to the Apc11 subunit of anaphase-promoting complex. Conditional inactivation of hrt1(ts) results in stabilization of the SCFCdc4 substrates Sic1 and Cln2 and cell cycle arrest at G1/S. Hrt1 assembles into recombinant SCF complexes and individually binds Cdc4, Cdc53 and Cdc34, but not Skp1. Hrt1 stimulates the E3 activity of recombinant SCF potently and enables the reconstitution of Cln2 ubiquitination by recombinant SCFGrr1. Surprisingly, SCF and the Cdc53/Hrt1 subcomplex activate autoubiquitination of Cdc34 E2 enzyme by a mechanism that does not appear to require a reactive thiol. The highly conserved human HRT1 complements the lethality of hrt1Delta, and human HRT2 binds CUL-1. We conclude that Cdc53/Hrt1 comprise a highly conserved module that serves as the functional core of a broad variety of heteromeric ubiquitin ligases. (+info)
Glial fibrillary acidic protein
Cullen DK, Simon CM, LaPlaca MC (July 2007). "Strain rate-dependent induction of reactive astrogliosis and cell death in three- ... Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is a type III intermediate ... GFAP can polymerize with other type III proteins. GFAP and other type III IF proteins cannot assemble with keratins, the type I ... "A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins". The Journal of ...
Ubiquitin-like protein
NEDD8 is best known for its role in regulating cullin proteins, which in turn regulate ubiquitin-mediated protein degradation, ... Ubiquitin-like proteins (UBLs) are a family of small proteins involved in post-translational modification of other proteins in ... "The dual role of ubiquitin-like protein Urm1 as a protein modifier and sulfur carrier". Protein & Cell. 2 (8): 612-9. doi: ... One additional protein, known as FUBI, is encoded as a fusion protein in the FAU gene, and is proteolytically processed to ...
HIV Rev response element
... stabilized by protein-protein interactions with other Rev molecules (in addition to the RNA-protein interactions). Biochemical ... Ahmed, Y F; Hanly, S M; Malim, M H; Cullen, B R; Greene, W C (1990). "Structure-function analyses of the HTLV-I Rex and HIV-1 ... Assembly of this Rev-RRE complex is followed by the recruitment of a human protein complex containing the proteins exportin-1 ( ... In the presence of the HIV-1 accessory protein Rev, HIV-1 mRNAs that contain the RRE can be exported from the nucleus to the ...
Vesicular transport adaptor protein
Burd C, Cullen PJ (2014). "Retromer: a master conductor of endosome sorting". Cold Spring Harbor Perspectives in Biology. 6 (2 ... In the AP complexes, there are two large proteins (∼100 kD) and two smaller proteins. One of the large proteins is termed β ( ... Protein complexes, Protein families, Molecular evolution, Vesicular transport proteins). ... PTBs are protein domains that include NUMB, DAB1 and DAB2. Epsin and AP180 in the ANTH domain are other adaptor proteins that ...
S-phase kinase-associated protein 1
Ng RW, Arooz T, Yam CH, Chan IW, Lau AW, Poon RY (1998). "Characterization of the cullin and F-box protein partner Skp1". FEBS ... This gene encodes a protein that is a member of the SCF ubiquitin ligase protein complex. It binds to F-box proteins (proteins ... Ng RW, Arooz T, Yam CH, Chan IW, Lau AW, Poon RY (November 1998). "Characterization of the cullin and F-box protein partner ... The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level ...
F-box protein 40
SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases ... F-box protein 40 is a protein that in humans is encoded by the FBXO40 gene. Fbxo40 induces ubiqitination of IRS1, thus limiting ... F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein ... Fbxo40 in particular engages a unique substrate, IRS1, which is a protein in the IGF1 signaling pathway. In this way, Fbxo40 ...
FBXW10
SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603034), and F-box proteins, act as protein-ubiquitin ligases ... F-box/WD repeat-containing protein 10 is a protein that in humans is encoded by the FBXW10 gene. Members of the F-box protein ... F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein ... 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs ...
FBXO28
SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases ... F-box only protein 28 is a protein that in humans is encoded by the FBXO28 gene. Members of the F-box protein family, such as ... F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode: ...
FBXO31
SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases ... F-box only protein 31 is a protein that in humans is encoded by the FBXO31 gene. Members of the F-box protein family, such as ... F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein ... the F-box protein FBXO31, a candidate tumor suppressor encoded in 16q24.3 for which there is loss of heterozygosity in various ...
GPS1
2003). "Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome". EMBO J. 22 (6): 1302-12. doi:10.1093 ... an assembly and maintenance platform for cullin ubiquitin ligases?". Nat. Cell Biol. 5 (12): 1029-33. doi:10.1038/ncb1203-1029 ... This gene is known to suppress G-protein and mitogen-activated signal transduction in mammalian cells. The encoded protein ... 1997). "Two human cDNAs, including a homolog of Arabidopsis FUS6 (COP11), suppress G-protein- and mitogen-activated protein ...
NEDD8
Pan ZQ, Kentsis A, Dias DC, Yamoah K, Wu K (March 2004). "Nedd8 on cullin: building an expressway to protein destruction". ... Liakopoulos D, Büsgen T, Brychzy A, Jentsch S, Pause A (May 1999). "Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 ... Morimoto M, Nishida T, Honda R, Yasuda H (April 2000). "Modification of cullin-1 by ubiquitin-like protein Nedd8 enhances the ... NEDD8 is a protein that in humans is encoded by the NEDD8 gene. (in saccharomyces cerevisiae this protein is known as Rub1) ...
CUL2
Cullin-2 is a protein that in humans is encoded by the CUL2 gene. CUL2 has been shown to interact with: CAND1, DCUN1D1, RBX1, ... "Entrez Gene: CUL2 cullin 2". Menon S, Tsuge T, Dohmae N, Takio K, Wei N (2008). "Association of SAP130/SF3b-3 with Cullin-RING ... structural resemblance of the pVHL/elongin BC/hCUL-2 complex with the ubiquitin ligase complex SKP1/cullin/F-box protein". Proc ... "Covalent modification of all members of human cullin family proteins by NEDD8". Oncogene. 18 (48): 6829-34. doi:10.1038/sj.onc. ...
CUL4B
Cullin-4B is a protein that in humans is encoded by the CUL4B gene which is located on the X chromosome. CUL4B has high ... protein is a CRL4 substrate only when directed by viral proteins §protein is a CRL4 substrate only when directed by IMiDs ... At the C-terminal end, CUL4B interacts with the RBX1/ROC1 protein via its RING domain. RBX1 is a core component of Cullin-RING ... This modification appears to induce conformational changes which promotes flexibility in the RING domain of cullin proteins and ...
CUL4A
Cullin-4A is a protein that in humans is encoded by the CUL4A gene. CUL4A belongs to the cullin family of ubiquitin ligase ... protein is a CRL4A substrate only when directed by viral proteins §protein is a CRL4A substrate only when directed by IMiDs ... At the C-terminal end, CUL4A interacts with the RBX1/ROC1 protein via its RING domain. RBX1 is a core component of Cullin-RING ... Shiyanov P, Nag A, Raychaudhuri P (Dec 1999). "Cullin 4A associates with the UV-damaged DNA-binding protein DDB". The Journal ...
Butterfly
Harpel, D.; Cullen, D. A.; Ott, S. R.; Jiggins, C. D.; Walters, J. R. (2015). "Pollen feeding proteomics: Salivary proteins of ... During this phase, the cuticle, a tough outer layer made of a mixture of chitin and specialized proteins, is released from the ... it begins as a pale yellow granular secretion containing acidophilic proteins. This is viscous and darkens when exposed to air ...
APPBP1
"Covalent modification of all members of human cullin family proteins by NEDD8". Oncogene. 18 (48): 6829-34. doi:10.1038/sj.onc. ... The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface ... APPBP1 (Amyloid Precursor Protein-Binding Protein 1) binds to the Amyloid Precursor Protein (APP) carboxy terminal domain. ... In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This ...
Biotinylation
Cull, M. G.; Schatz, P. J. (2000-01-01). Biotinylation of proteins in vivo and in vitro using small peptide tags. Methods in ... More recently, in vivo protein biotinylation was developed to study protein-protein interactions and proximity in living cells ... the purification of biotinylated proteins has been a widely used approach to identify protein-protein interactions and post- ... If isolation of the tagged protein is needed, it is better to tag the protein with iminobiotin. This biotin analogue gives ...
Lipid bilayer
Chonn A, Semple SC, Cullis PR (15 September 1992). "Association of blood proteins with large unilamellar liposomes in vivo. ... Some of these proteins are linked to the exterior of the cell membrane. An example of this is the CD59 protein, which ... The most common class of this type of protein is the G protein-coupled receptor (GPCR). GPCRs are responsible for much of the ... The first of these proteins to be studied were the viral fusion proteins, which allow an enveloped virus to insert its genetic ...
Cullin
The RING protein appears to function as a docking site for ubiquitin-conjugating enzymes (E2s). Other proteins contain a cullin ... Cullin family - Sanger Institute website. Cullin+Proteins at the US National Library of Medicine Medical Subject Headings (MeSH ... The catalytic core of CRLs consists of a RING protein and a cullin family member. For Cul1, the C-terminal cullin-homology ... Cullin-RING ubiquitin ligases (CRLs), such as Cul1 (SCF) play an essential role in targeting proteins for ubiquitin-mediated ...
Hemoglobin
Each subunit is composed of a protein chain tightly associated with a non-protein prosthetic heme group. Each protein chain ... Perutz, M.F.; Rossmann, M.G.; Cullis, A.F.; Muirhead, H.; Will, G.; North, A.C.T. (1960). "Structure of haemoglobin: a three- ... In all proteins, it is the amino acid sequence that determines the protein's chemical properties and function. There is more ... Hemoglobin consists of protein subunits (the globin molecules), and these proteins, in turn, are folded chains of a large ...
CUL1
Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein ... Morimoto M, Nishida T, Honda R, Yasuda H (2000). "Modification of cullin-1 by ubiquitin-like protein Nedd8 enhances the ... 1998). "Characterization of the cullin and F-box protein partner Skp1". FEBS Lett. 438 (3): 183-9. doi:10.1016/S0014-5793(98) ... 2000). "Covalent modification of all members of human cullin family proteins by NEDD8". Oncogene. 18 (48): 6829-34. doi:10.1038 ...
CUL5
Cullin-5 is a protein that in humans is encoded by the CUL5 gene. The mammalian gene product was originally discovered by ... Since then, VACM-1 has been shown to be homologous to the Cullin family of proteins, and was subsequently dubbed cul5. Studies ... Feng L, Allen NS, Simo S, Cooper JA (November 2007). "Cullin 5 regulates Dab1 protein levels and neuron positioning during ... 2000). "Covalent modification of all members of human cullin family proteins by NEDD8". Oncogene. 18 (48): 6829-34. doi:10.1038 ...
SKP2
The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), ... Ng RW, Arooz T, Yam CH, Chan IW, Lau AW, Poon RY (November 1998). "Characterization of the cullin and F-box protein partner ... and Fbxos containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by ... Yam CH, Ng RW, Siu WY, Lau AW, Poon RY (January 1999). "Regulation of cyclin A-Cdk2 by SCF component Skp1 and F-box protein ...
Leptospira
Cullen PA, Cordwell SJ, Bulach DM, Haake DA, Adler B (2002). "Global Analysis of Outer Membrane Proteins from Leptospira ... The outer membrane contains a variety of lipoproteins and transmembrane outer membrane proteins. As expected, the protein ... Bulach DM, Zuerner RL, Wilson P, Seemann T, McGrath A, Cullen PA, Davis J, Johnson M, Kuczek E, Alt DP, Peterson-Burch B, ... The HbpA hemin-binding protein, which may be involved in the uptake of hemin, has been identified on the surface of L. ...
Retromer
However, it is clear that there are other complexes and proteins that act in this retrieval process. So far it is not clear ... Wassmer T, Attar N, Bujny MV, Oakley J, Traer CJ, Cullen PJ (January 2007). "A loss-of-function screen reveals SNX5 and SNX6 as ... Burd C, Cullen PJ (February 2014). "Retromer: a master conductor of endosome sorting". Cold Spring Harbor Perspectives in ... The cargo carrier is transported to the TGN by motor proteins such as dynein. Tethering of the cargo carrier to the recipient ...
Armadillo repeat
But, the armadillo repeat is found in a wide range of proteins with other functions. This type of protein domain is important ... Herold A, Truant R, Wiegand H, Cullen BR (October 1998). "Determination of the functional domain organization of the importin ... Although β-catenin was previously believed to be a protein involved in linking cadherin cell adhesion proteins to the ... repetitive amino acid sequence of about 40 residues in length that is found in many proteins. Proteins that contain armadillo ...
F-box protein 15
SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 ... F-box protein 15 also known as Fbx15 is a protein that in humans is encoded by the FBXO15 gene. Members of the F-box protein ... Differently from other F-box proteins, Fbx15 recognizes its substrate Kif1-Binding Protein (KBP) in an acetylation dependent ... Fbxo15 is a protein expressed in undifferentiated embryonic stem cells. It is expressed during coexpression of Oct3/4, c-Myc, ...
Kelch protein
Kelch-like proteins are known to act as substrate adaptors for Cullin 3 ubiquitin ligases. The first Kelch protein (from which ... Kelch proteins (and Kelch-like proteins) are a widespread group of proteins that contain multiple Kelch motifs. The kelch ... The BTB domain of kelch proteins (if present) allows the formation of homo- or heterodimers that mediate protein-protein ... The N-terminus of several Kelch proteins contain other protein domains, including Discoidin, F-box, and Broad-complex, ...
Neddylation
Because NEDD8 conjugation to cullin proteins plays an important role in the regulation of the cell cycle, an upregulation in ... Second, it can cause the target protein to become incompatible with other proteins that it usually binds with. For example, ... Neddylation (also NEDDylation) is the process by which the ubiquitin-like protein NEDD8 is conjugated to its target proteins. ... Herrmann J, Lerman LO, Lerman A. Ubiquitin and ubiquitin-like proteins in protein regulation. Circ Res. 2007;100(9):1276-91. ...
Suppressyn
It was originally a gene for a viral coat protein that has become stabily integrated within the genomes of humans and other ... Frank JA, Singh M, Cullen HB, Kirou RA, Benkaddour-Boumzaouad M, Cortes JL, et al. (October 2022). "Evolution and antiviral ... Suppressyn (SUPYN) is a protein that in humans is encoded by the ERVH48-1 (endogenous retrovirus group 48 member 1) gene. ... The function of this endogenous retrovirus protein is possibly to protect against infection by mammalian type D retroviruses. ...
PARC (gene)
Cullin-9 is a protein that in humans is encoded by the CUL9 gene. PARC (gene) has been shown to interact with P53. GRCh38: ... Nakayama M, Kikuno R, Ohara O (2003). "Protein-Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a ... 2007). "The conserved CPH domains of Cul7 and PARC are protein-protein interaction modules that bind the tetramerization domain ... X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169-76 ...
Short interspersed nuclear element
Furthermore, SINEs frequently contain motifs for YY1 polycomb proteins. YY1 is a zinc-finger protein that acts as a ... Cai X, Hagedorn CH, Cullen BR (December 2004). "Human microRNAs are processed from capped, polyadenylated transcripts that can ... Thereafter, one of the strands is incorporated into a multi-protein RNA-induced silencing complex (RISC). Among these proteins ... encodes a protein which binds to RNA and acts as a chaperone to facilitate and maintain the LINE protein-RNA complex structure ...
Zostera marina
Jones, BJ; Cullen-Unsworth, LC; Unsworth, RKF (2018). "Tracking Nitrogen Source Using δ15N Reveals Human and Agricultural ... The approximate genome sequence of Z. marina is 202.3 Mb and encodes approximately 20450 protein-coding genes (of which 86,6% ...
WNK1
The WNK1 protein is composed of 2382 amino acids (molecular weight 230 kDa). The protein contains a kinase domain located ... There are two enzymes responsible for WNK1 ubiquitination, kelch like 3 (KLHL3) and cullin 3 (CUL3). KLHL3 serves as an adaptor ... The gene belongs to a group of four related protein kinases (WNK1, WNK2, WNK3, WNK4). Homologs of this protein have been found ... WNK (lysine deficient protein kinase 1), also known as WNK1, is an enzyme that is encoded by the WNK1 gene. WNK1 is serine- ...
Cereblon
... forms an E3 ubiquitin ligase complex with damaged DNA binding protein 1 (DDB1), Cullin-4A (CUL4A), and regulator of ... Cereblon is a protein that in humans is encoded by the CRBN gene. The gene that encodes the cereblon protein is found on the ... CRBN protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) Overview of all the structural ... As they report in the current issue of Science, a protein known as cereblon latched on tightly to the thalidomide. "Thalidomide ...
DDB1
"The cullin 4B-based UV-damaged DNA-binding protein ligase binds to UV-damaged chromatin and ubiquitinates histone H2A". Cancer ... DNA damage-binding protein 1 is a protein that in humans is encoded by the DDB1 gene. The gene's position is on chromosome ... DDB1 serves as a bridge or adaptor protein which interacts with dozens of proteins known as DDB1 and CUL4-associated factors ( ... Leupin O, Bontron S, Strubin M (2003). "Hepatitis B virus X protein and simian virus 5 V protein exhibit similar UV-DDB1 ...
Mauro Ferrari
Dead Sea Research Institute Engineering Leadership Board Cullen College of Engineering, University of Houston. "Mauro Ferrari ... "Particles for oral delivery of peptides and proteins", published 2002 US patent 6405066, Essenpreis M, Desai TA, Ferrari M, ... Cullen College of Engineering, University of Houston Ferrari's research uses nanotechnology, microtechnology, physical sciences ...
SNX8
... are involved in protein-protein interactions. The SNX8 protein, even though is very similar to the other sorting nexins, ... van Weering, Jan R.T.; Verkade, Paul; Cullen, Peter J. (January 2012). "SNX-BAR-Mediated Endosome Tubulation is Co-ordinated ... SNX8 protein regulates cholesterol levels as an activator of the SREBPs (Sterol Regulatory Element Binding Proteins), which is ... As a protein of the SNXs family, the SNX8 is formed of 465 aminoacids and presents a BAR-domain and a PX-domain which are very ...
African swine fever virus
Viral membrane proteins with similarity to cellular adhesion proteins modulate interaction of virus-infected cells and ... that resulted in a cull of around 5,000 sows and piglets by using gas. In February, another massive pig cull was required, ... structural proteins, and many proteins that are not essential for replication in cells, but instead have roles in virus ... China culls 900 pigs after reports of first African swine fever outbreak in country. "China says has culled over 38,000 hogs ...
Plasma medicine
... proteins, carbohydrates, lipids, and amino acids Plasma-assisted prodrug activation One of challenges is the application of non ... Cullen, Patrick J.; Curtin, James F. (24 April 2020). "Cold Atmospheric Plasma Stimulates Clathrin-Dependent Endocytosis to ...
Methylation
Protein methylation typically takes place on arginine or lysine amino acid residues in the protein sequence. Arginine can be ... Styblo, M.; Del Razo, L. M.; Vega, L.; Germolec, D. R.; LeCluyse, E. L.; Hamilton, G. A.; Reed, W.; Wang, C.; Cullen, W. R.; ... Walsh, Christopher (2006). "Chapter 5 - Protein Methylation" (PDF). Posttranslational modification of proteins: expanding ... Protein methylation is one type of post-translational modification. Methyl metabolism is very ancient and can be found in all ...
2019 in New Zealand
Dave Cull, then from 25 October Aaron Hawkins Phil Goff Greg Brownless Andrew King Paula Southgate Justin Lester Andy Foster ... protein chemist (born 1937) 8 September - Ray Hitchcock, cricketer, racehorse breeder (born 1929) 9 September - Alister Taylor ... Lianne Dalziell Dave Cull Aaron Hawkins 5 February - 6 March - Wildfires in Nelson cause thousands to evacuate their homes. 15 ...
Per- and polyfluoroalkyl substances
Jones PD, Hu W, De Coen W, Newsted JL, Giesy JP (November 2003). "Binding of perfluorinated fatty acids to serum proteins". ... Sakr CJ, Leonard RC, Kreckmann KH, Slade MD, Cullen MR (August 2007). "Longitudinal study of serum lipids and liver enzymes in ... They have been shown to bind to blood proteins and accumulate in the livers of marine animals. Another pathway for ... In particular, IgA, IgE (in females only) and C-reactive protein have been shown to decrease whereas antinuclear antibodies ...
Cyclin T2
Cyclin-T2 is a protein that in humans is encoded by the CCNT2 gene. The protein encoded by this gene belongs to the highly ... Bieniasz PD, Grdina TA, Bogerd HP, Cullen BR (1999). "Analysis of the effect of natural sequence variation in Tat and in cyclin ... Kwak YT, Ivanov D, Guo J, Nee E, Gaynor RB (1999). "Role of the human and murine cyclin T proteins in regulating HIV-1 tat- ... Jang MK, Mochizuki K, Zhou M, Jeong HS, Brady JN, Ozato K (2005). "The bromodomain protein Brd4 is a positive regulatory ...
Dicer
Plant genomes encode for dicer like proteins with similar functions and protein domains as animal and insect dicer. For example ... ISBN 978-0-8053-9592-1. Zeng Y, Yi R, Cullen BR (Aug 2003). "MicroRNAs and small interfering RNAs can inhibit mRNA expression ... The protein size is 82 kDa, representing the conserved functional core that was subsequently been found in larger Dicer ... As an example, Drosophila C virus encodes for protein 1A which binds to dsRNA thus protecting it from dicer cleavage as well as ...
Cat coat genetics
The d allele is a single-base deletion that truncates the protein. If the cat has d/d genes, the coat is diluted. If the genes ... Webb AA, Cullen CL (June 2010). "Coat color and coat color pattern-related neurologic and neuro-ophthalmic diseases". Can. Vet ... In cheetahs, a base pair insertion into exon 20 of the protein replaces the 16 C-terminal residues with 109 new ones ( ... The Dense pigment gene, D/d, codes for melanophilin (MLPH; A0SJ36), a protein involved in the transportation and deposition of ...
Camel
Demand is outstripping supply, and governments are being urged not to cull the camels, but redirect the cost of the cull into ... Until recently, camel milk could not be made into camel cheese because rennet was unable to coagulate the milk proteins to ... On the other hand, camel milk and meat are rich in protein, vitamins, glycogen, and other nutrients making them essential in ... Organization of membrane proteins". Biochimica et Biophysica Acta (BBA) - Biomembranes. 426 (4): 647-58. doi:10.1016/0005-2736( ...
Ubiquitin
The anaphase-promoting complex (APC) and the SCF complex (for Skp1-Cullin-F-box protein complex) are two examples of multi- ... coordinating the cellular localization of proteins, activating and inactivating proteins, and modulating protein-protein ... The protein modifications can be either a single ubiquitin protein (monoubiquitylation) or a chain of ubiquitin ( ... The monoubiquitination of a protein can have different effects to the polyubiquitination of the same protein. The addition of a ...
Dysferlin
The human dysferlin protein is a 237 kilodalton type-II transmembrane protein. It contains a large intracellular cytoplasmic N- ... Anderson LV, Davison K, Moss JA, Young C, Cullen MJ, Walsh J, Johnson MA, Bashir R, Britton S, Keers S, Argov Z, Mahjneh I, ... Dysferlin also known as dystrophy-associated fer-1-like protein is a protein that in humans is encoded by the DYSF gene. ... Matsuda C, Aoki M, Hayashi YK, Ho MF, Arahata K, Brown RH (1999). "Dysferlin is a surface membrane-associated protein that is ...
List of University of Wisconsin-Madison people
"Henry Cullen Adams". Biographical Directory of the United States Congress. Retrieved 1 December 2013. "Iajuddin Ahmed". ... for describing the genetic code and how it operates in protein synthesis Franklin Hiram King, soil scientist and early promoter ... for describing the genetic code and how it operates in protein synthesis Virgil Abloh, fashion designer, artistic director of ... Chief Justice of the Wisconsin Supreme Court Henry Cullen Adams, U.S. Representative Iajuddin Ahmed, former President of ...
Nucleoporin 214
The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for ... Bogerd HP, Echarri A, Ross TM, Cullen BR (Nov 1998). "Inhibition of human immunodeficiency virus Rev and human T-cell leukemia ... Nucleoporin 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene. The nuclear pore complex is a massive ... Kraemer D, Wozniak RW, Blobel G, Radu A (Feb 1994). "The human CAN protein, a putative oncogene product associated with myeloid ...
P120 (protein)
Wahl JK, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ (May 2003). "N-cadherin-catenin complexes form prior to cleavage of the ... p120, and called catenin delta-1 is a protein that in humans is encoded by the CTNND1 gene. This gene encodes a member of the ... The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 4 (2): 141-50. ... "Entrez Gene: CTNND1 catenin (cadherin-associated protein), delta 1". Schackmann, R. C. J.; Tenhagen, M.; van de Ven, R. A. H.; ...
MicroRNA
... binding protein], PACT (protein activator of the interferon-induced protein kinase), the SMN complex, fragile X mental ... Cai X, Hagedorn CH, Cullen BR (December 2004). "Human microRNAs are processed from capped, polyadenylated transcripts that can ... HMGA proteins (HMGA1a, HMGA1b and HMGA2) are implicated in cancer, and expression of these proteins is regulated by microRNAs. ... HMGA2 protein specifically targets the promoter of ERCC1, thus reducing expression of this DNA repair gene. ERCC1 protein ...
Bison hunting
In this way, the hunting is used as a part of the wildlife management strategy and to help cull less desirable individuals.[ ... Bison is high in protein levels and low in fat content, and contributed to the wholesome diet of Native Americans. Additionally ...
Seagrass
Arabinogalactan proteins are ubiquitous in seed land plants and have also been found in ferns, lycophytes and mosses. They are ... Unsworth, Richard K. F.; McKenzie, Len J.; Collier, Catherine J.; Cullen-Unsworth, Leanne C.; Duarte, Carlos M.; Eklöf, Johan S ... Nordlund, L. M.; Unsworth, R. K. F.; Gullstrom, M.; Cullen-Unsworth, L. C. (2018). "Global significance of seagrass fishery ... Unsworth, Richard K. F.; Cullen-Unsworth, Leanne C.; Jones, Benjamin L.; Lilley, Richard J. (5 August 2022). "The planetary ...
SCF complex
Skp1 - Skp1 is an adaptor protein that is essential for the recognition and binding of F-box proteins. Cullin (CUL1) - Cullin ... Skp, Cullin, F-box containing complex (or SCF complex) is a multi-protein E3 ubiquitin ligase complex that catalyzes the ... The SCF complex also marks various other cellular proteins for destruction. SCF contains a variable F-box protein and three ... Instead, FBP affinity for protein substrates is regulated through cyclin-CDK-mediated phosphorylation of target proteins. ...
Signalosome
COP9 signalosome: Catalyses the hydrolysis of NEDD8 protein from the Cullin subunit of Cullin-RING ubiquitin ligases (CRL). ... v t e (Protein complexes, All stub articles, Protein stubs). ... Sear, Richard P. (2007). "Dishevelled: a protein that functions ... Schwarz-Romond, T.; Metcalfe, C.; Bienz, M. (2007). "Dynamic recruitment of axin by Dishevelled protein assemblies". Journal of ... Schwarz-Romond, T. (2005). "The Wnt signalling effector Dishevelled forms dynamic protein assemblies rather than stable ...
Tat (HIV)
In molecular biology, Tat is a protein that is encoded for by the tat gene in HIV-1. Tat is a regulatory protein that ... Hauber J, Malim MH, Cullen BR (March 1989). "Mutational analysis of the conserved basic domain of human immunodeficiency virus ... 2001). "Protein Transduction Domain of HIV-1 Tat Protein Promotes Efficient Delivery of DNA into Mammalian Cells". Journal of ... RNA binding proteins. Tat contains a protein transduction domain (PTD), which is also known as a cell-penetrating peptide. ...
Cullin Proteins | Profiles RNS
"Cullin Proteins" by people in this website by year, and whether "Cullin Proteins" was a major or minor topic of these ... "Cullin Proteins" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Cullin Proteins" by people in Profiles. ... Below are MeSH descriptors whose meaning is more general than "Cullin Proteins". ...
Function and regulation of cullin-RING ubiquitin ligases
Owing to the great diversity of their substrate-receptor subunits, it is possible that there are hundreds of distinct cullin- ... Cullin-RING complexes comprise the largest known class of ubiquitin ligases. ... which establishes these enzymes as key mediators of post-translational protein regulation. In this review, we focus on the ... Function and regulation of cullin-RING ubiquitin ligases Nat Rev Mol Cell Biol. 2005 Jan;6(1):9-20. doi: 10.1038/nrm1547. ...
CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression -...
Here, we found that CUL3 (cullin 3), an E … ... A) The protein lysates of the indicated cell lines were ... A Destiny for Degradation: Interplay between Cullin-RING E3 Ligases and Autophagy. Lu G, Wang L, Zhou J, Liu W, Shen HM. Lu G, ... A) BECN1 protein or mRNA downregulation overlap in different tumor samples. (B) The low expression of BECN1 was not due to the ... CUL3 (cullin 3)-mediated ubiquitination and degradation of BECN1 (beclin 1) inhibit autophagy and promote tumor progression ...
Sequence and structural analysis of BTB domain proteins | Genome Biology | Full Text
We surveyed the protein architecture, genomic distribution and sequence conservation of BTB domain proteins in 17 fully ... families of proteins, among others. In contrast, the Skp1 and ElonginC proteins consist almost exclusively of the core BTB fold ... is a versatile protein-protein interaction motif that participates in a wide range of cellular functions, including ... MATH-BTB proteins in Caenorhabditis elegans, and BTB-NPH3 proteins in Arabidopsis thaliana. Using the structural homology ...
RCSB PDB - 7SHK: Structure of Xenopus laevis CRL2Lrr1 (State 1)
CULLIN_2 domain-containing protein. A. 745. Xenopus laevis. Mutation(s): 0 Gene Names: cul2.L, cul2, XELAEV_18030721mg. ... Tceb2-prov protein. B. 119. Xenopus laevis. Mutation(s): 0 Gene Names: elob.L, elob, tceb2, elongin-B, elonginb, siii, tceb2.L ... RING-type domain-containing protein. E [auth R]. 108. Xenopus laevis. Mutation(s): 0 Gene Names: XELAEV_18023559mg, rbx1.L. ...
Table 3 - Candidate Cell Substrates, Vaccine Production, and Transmissible Spongiform Encephalopathies - Volume 17, Number 12...
CUL3-related neurodevelopmental disorder: MedlinePlus Genetics
This gene provides instructions for making a protein called cullin-3. The cullin-3 protein plays a role in the ubiquitin- ... no cullin-3 protein production at all. Without the normal amount of functioning cullin-3 proteins, the breakdown of unwanted ... Since cullin-3 is produced in nerve cells throughout the brain, it is likely that the proteins dysfunction damages nerve cells ... and excess proteins. By breaking down proteins at the right time, this system regulates the timing of critical cell activities ...
CUL2 - Gene - NCBI
cullin 2 [Macaca mulatta (Rhesus monkey)]. Chromosome 9, NC_041762.1 (35966859..36053768, complement). EGK_19580, Cullin-2. ... Protein. aa. Isoform. Status. NM_003591.4 4,183. NP_003582.2 745. c. MANE Select ... cullin 2 [Gallus gallus (chicken)]. Chromosome 2, NC_052533.1 (12973325..13019364). cullin-2. ... Cullin 2 [Drosophila melanogaster (fruit fly)]. Chromosome 2L, NT_033779.5 (21658735..21663698). Dmel_CG1512, CG1512, CUL2, Cul ...
CUL1 ORF clone FXC04681 - KOP/KDRI
Cullin protein. HMMSmart. IPR016158. 447. 594. SM00182. Cullin homology. IPR019559. 703. 770. SM00884. Cullin protein. ... ORF Protein. Cloned ORF protein sequence for pF1KB8598 Download >KIBB8598 776 aa ... Result of homology search against CCDS protein database ( FASTA output, Multiple alignment). ...
Biomarkers Search
The cullin protein family.. Sarikas A; Hartmann T; Pan ZQ. Genome Biol; 2011; 12(4):220. PubMed ID: 21554755. [TBL] ... BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase.. Furukawa M; ... Characterization of cullin-based E3 ubiquitin ligases in intact mammalian cells--evidence for cullin dimerization.. Chew EH; ... Cullin 3 and Its Role in Tumorigenesis.. Chen RH. Adv Exp Med Biol; 2020; 1217():187-210. PubMed ID: 31898229. [TBL] ...
Understanding amyloid fibril formation using protein fragments: structural investigations via vibrational spectroscopy and...
To solve this problem, several research groups have chosen to focus on short fragments of amyloid proteins, sequences that have ... Studying short peptides allows bypassing the complexity of working with full-length proteins and may provide important ... diseases are part of a wider family encompassing more than 50 human pathologies related to aggregation of proteins. Although ... information relative to critical segments of amyloid proteins. To this end, efficient biophysical tools are required. In this ...
Publication Detail
Adaptor Proteins, Signal Transducing/metabolism; Animals; Antioxidants/metabolism; Cullin Proteins/genetics; Cullin Proteins/ ... There are seven known Cullin proteins that form various CRL complexes. They are regulated by neddylation/deneddylation, ... Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/metabolism*; Ubiquitin/genetics; Ubiquitin/metabolism* ... Intracellular Signaling Peptides and Proteins/metabolism; Kelch-Like ECH-Associated Protein 1; NF-E2-Related Factor 2/genetics ...
Ultra-processed Foods, Weight Gain, and Co-morbidity Risk | SpringerLink
Fermentation | Free Full-Text | Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans
These cell wall proteins are capable of recognizing protein ligands [16], glycolipids [19,20,21,22] and carbohydrates [23,24,25 ... McCall, A.D.; Pathirana, R.U.; Prabhakar, A.; Cullen, P.J.; Edgerton, M. Candida albicans biofilm development is governed by ... de Groot, P.W.J.; Hellingwerf, K.J.; Klis, F.M. Genome-wide identification of fungal GPI proteins. Yeast 2003, 20, 781-796. [ ... In a first adhesion step, the C. albicans adhesins of the agglutinin-like sequence (Als) family bind to ECM proteins of the ...
Modified BCG vaccine could prevent TB in cattle and help end culls | New Scientist
SSSNFL H
Neurofilament light protein in blood as a potential biomarker of neurodegeneration in Huntingtons disease: a retrospective ... Olsson B, Portelius E, Cullen NC, Sandelius Å, Zetterberg H, Andreasson U, et al. Association of Cerebrospinal Fluid ... Neurofilaments are the dominant proteins of the neural cytoskeleton and are released into the extracellular space following ... Neurofilament Light Protein Levels With Cognition in Patients With Dementia, Motor Neuron Disease, and Movement Disorders. JAMA ...
NIOSHTIC-2 Search Results - Full View
Animal Model and Therapeutics Evaluation Core
Biomarkers Search
1. Coordinated Actions Between p97 and Cullin-RING Ubiquitin Ligases for Protein Degradation.. Shi W; Ding R; Zhou PP; Fang Y; ... Cullin 3SPOP ubiquitin E3 ligase promotes the poly-ubiquitination and degradation of HDAC6.. Tan Y; Ci Y; Dai X; Wu F; Guo J; ... Regulation of cullin RING ligases.. Hotton SK; Callis J. Annu Rev Plant Biol; 2008; 59():467-89. PubMed ID: 18444905. [TBL] ... 8. NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway.. den Besten W; Verma R; Kleiger G; Oania RS; Deshaies ...
SCOPe 2.07: Domain d3dplr : 3dpl R
Family g.44.1.1: RING finger domain, C3HC4 [57851] (15 proteins). *. Protein RIGG-box protein 1 (RBX1) of SCF ubiquitin ligase ... PDB Description: structural insights into nedd8 activation of cullin-ring ligases: conformational control of conjugation. ... PDB Compounds: (R:) RING-box protein 1. SCOPe Domain Sequences for d3dplr_:. Sequence, based on SEQRES records: (download). > ... Class g: Small proteins [56992] (98 folds). *. Fold g.44: RING/U-box [57849] (1 superfamily). dimetal(zinc)-bound alpha+beta ...
Ancient viral DNA may help humans fight infections | National Institutes of Health (NIH)
Researchers found that stretches of viral DNA long embedded in the human genome can produce proteins that help block infection ... References: Evolution and antiviral activity of a human protein of retroviral origin. Frank JA, Singh M, Cullen HB, Kirou RA, ... Among the potentially useful DNA snippets from viruses are those encoding envelope proteins. Envelope proteins are normally ... "The results show that in the human genome, we have a reservoir of proteins that have the potential to block a broad range of ...
Brenda Schulman, PhD - St. Jude Children's Research Hospital
The glomuvenous malformation protein glomulin binds Rbx1 and regulates cullin RING ligase-mediated turnover of Fbw7. Molecular ... A RING E3-substrate complex poised for ubiquitin-like protein transfer: structural insights into cullin-RING ligases. Nat ... Twists and turns in ubiquitin-like protein conjugation cascades. Protein Sci 20(12):1941-54, 2011. ... Cullin neddylation and substrate-adaptors counteract SCF inhibition by the CAND1-like protein Lag2 in Saccharomyces cerevisiae ...
Frontiers | Regulation of Dynamic Protein S-Acylation
... protein interactions, protein stability, and protein conformation. This process is regulated by palmitoyl acyltransferases that ... protein interactions, protein stability, and protein conformation. This process is regulated by palmitoyl acyltransferases that ... In this review, we will explore the recent findings on protein S-acylation, the enzymatic regulation of this process, and ... In this review, we will explore the recent findings on protein S-acylation, the enzymatic regulation of this process, and ...
TMPL - Publications
Cai, J., Zhao, Y., Glasier, J., Cullen, D., Barnes, S., Turner, C., and Weaver, C. Comparative effect of soy protein, soy ... In vivo protein sampling using capillary ultrafiltration semi-permeable hollow fiber and protein identification via mass ... The effect of isolated soy protein on plasma biomarkers in elderly men with elevated serum prostate specific antigen.. Journal ... Effects of soy on proteins implicated in dementias and other neurodegenerative conditions: the use of proteomics technology to ...
Protein modification as a tool for reprogramming gene activity - Research output - University of Edinburgh Research...
MeSH Browser
Cullin Cullin Domain Protein Cullin Domain Proteins Cullin Protein Cullins Registry Number. 0. Public MeSH Note. 2004. History ... Ubiquitin-Protein Ligases [D08.811.464.938.750] * SKP Cullin F-Box Protein Ligases [D08.811.464.938.750.750] * Cullin Proteins ... Cullin Proteins Preferred Concept UI. M0446344. Registry Number. 0. Scope Note. A family of structurally related proteins that ... Amino Acids, Peptides, and Proteins [D12] * Proteins [D12.776] * Cell Cycle Proteins [D12.776.167] * Anaphase-Promoting Complex ...
LigaseLigasesDomain ProteinsDegradationGeneComplexesNEDD8Target proteinsUbiquitinationRegulationCUL3SubstratePathwaysPeptidesAggregationArabidopsisAbstractDescriptorSequencesGenesBindSkp1AmyloidHomeostasisEukaryoticRegulates2020InteractionsZincNeurodegenerativePost-translationalAspects of proteinPrionChromosomeMiceProgressionFunctionalMutantRoleMoleculeCompositionViralInteractStructuralCattleFunctionAttachmentComplexCell
Ligase13
- BTB-ZF factors recruit the E3 ligase cullin 3 to regulate lymphoid effector programs. (uchicago.edu)
- Recently, BTB proteins have been identified in screens for interaction partners of the Cullin (Cul)3 Skp1-Cullin-F-box (SCF)-like E3 ubiquitin ligase complex, with the BTB domain mediating recruitment of the substrate recognition modules to the Cul3 component of the SCF-like complex [ 18 - 20 ]. (biomedcentral.com)
- Nrf2 is negatively regulated by Keap1, a substrate adaptor protein for the Cullin3 (Cul3)-containing E3-ligase complex, which targets Nrf2 for ubiquitination and degradation by the ubiquitin proteasome system (UPS). (nih.gov)
- Regulation of Nrf2 by the Cul3-Keap1-E3 ligase provides strong evidence that tight regulation of Cullin-ring ligases (CRLs) is imperative to maintain cellular homeostasis. (nih.gov)
- 4. p97/VCP promotes Cullin-RING-ubiquitin-ligase/proteasome-dependent degradation of IκBα and the preceding liberation of RelA from ubiquitinated IκBα. (nih.gov)
- 8. NEDD8 links cullin-RING ubiquitin ligase function to the p97 pathway. (nih.gov)
- 10. Rotavirus NSP1 Associates with Components of the Cullin RING Ligase Family of E3 Ubiquitin Ligases. (nih.gov)
- 13. Cullin 3SPOP ubiquitin E3 ligase promotes the poly-ubiquitination and degradation of HDAC6. (nih.gov)
- Ubls are attached to protein targets by a series of molecular handoffs involving an E1 activating enzyme, an E2 conjugating enzyme (or Ubc), an E3 ligase, and the target. (stjude.org)
- TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes. (stjude.org)
- We report that KLHL6 assembles with CULLIN3 to form a functional CULLIN-Ring ubiquitin ligase. (nih.gov)
- Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. (nih.gov)
- The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. (nih.gov)
Ligases11
- They play important roles in regulation of the CELL CYCLE and as components of UBIQUITIN-PROTEIN LIGASES. (uchicago.edu)
- Cullin-RING complexes comprise the largest known class of ubiquitin ligases. (nih.gov)
- Owing to the great diversity of their substrate-receptor subunits, it is possible that there are hundreds of distinct cullin-RING ubiquitin ligases in eukaryotic cells, which establishes these enzymes as key mediators of post-translational protein regulation. (nih.gov)
- In this review, we focus on the composition, regulation and function of cullin-RING ligases, and describe how these enzymes can be characterized by a set of general principles. (nih.gov)
- Regulation of the Nrf2-Keap1 antioxidant response by the ubiquitin proteasome system: an insight into cullin-ring ubiquitin ligases. (nih.gov)
- 1. Coordinated Actions Between p97 and Cullin-RING Ubiquitin Ligases for Protein Degradation. (nih.gov)
- 2. Cullin-RING E3 Ubiquitin Ligases: Bridges to Destruction. (nih.gov)
- 7. Cullin 5-RING E3 ubiquitin ligases, new therapeutic targets? (nih.gov)
- 19. Roles of Cullin-RING Ubiquitin Ligases in Cardiovascular Diseases. (nih.gov)
- 20. Regulation of cullin RING ligases. (nih.gov)
- structural insights into nedd8 activation of cullin-ring ligases: conformational control of conjugation. (berkeley.edu)
Domain Proteins2
- We surveyed the protein architecture, genomic distribution and sequence conservation of BTB domain proteins in 17 fully sequenced eukaryotes. (biomedcentral.com)
- An earlier study [ 30 ] is consistent with many of the results presented here, and we contribute an expanded structure and genome-centric analysis of BTB domain proteins, with an emphasis on the scope of protein-protein interactions in these proteins. (biomedcentral.com)
Degradation3
- 6. NEDD8 ultimate buster-1 long (NUB1L) protein promotes transfer of NEDD8 to proteasome for degradation through the P97UFD1/NPL4 complex. (nih.gov)
- When their time has come, the gene Cullin 3 tags them for degradation - a process that has to be tightly regulated to prevent proteins from accumulating. (ist.ac.at)
- The HIV-1 Vpr accessory protein induces ubiquitin/proteasome-dependent degradation of many cellular proteins by recruiting them to a cullin4A-DDB1-DCAF1 complex. (elifesciences.org)
Gene10
- This gene provides instructions for making a protein called cullin-3. (medlineplus.gov)
- The CUL3 gene variants that cause CUL3 -related neurodevelopmental disorder can lead to the production of nonfunctional cullin-3 proteins, and in some cases, no cullin-3 protein production at all. (medlineplus.gov)
- nonchromosomal gene is a prion of the HET-s protein of This result is consistent with interaction of the prion domain the filamentous fungus Podospora anserina ( 2 ). (nih.gov)
- One of them is called Cullin 3, and it is a high-risk gene: A mutation of this gene almost certainly leads to a disorder. (ist.ac.at)
- To learn more about it, Jasmin Morandell and Lena Schwarz, PhD students at Professor Gaia Novarino's research group , turned to mice whose Cullin 3 gene has been partially deactivated and compared them to their healthy siblings. (ist.ac.at)
- The mouse with the altered Cullin 3 gene, however, showed no sign of recognition. (ist.ac.at)
- If the Cullin 3 gene is deactivated, the Plastin 3 protein accumulates, causing cells to migrate slower and over shorter distances. (ist.ac.at)
- Most egg allergies are caused by one of just four proteins in the white, and when Doran's colleagues altered the gene that encodes one of these in bacteria, the resulting protein no longer triggered a reaction in blood serum from people who were known to be allergic to it 1 . (nature.com)
- Destroying CCDC137 also mimicked the effects of Vpr on HIV-1 gene expression, increasing the levels of virus proteins in infected cells. (elifesciences.org)
- Kelch-like protein 6 (KLHL6) is an uncharacterized gene mutated in diffuse large B-cell lymphoma (DLBCL). (nih.gov)
Complexes3
- There are seven known Cullin proteins that form various CRL complexes. (nih.gov)
- 5. UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1α accumulation. (nih.gov)
- SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). (nih.gov)
NEDD81
- Structure of a RING E3 trapped in action reveals ligation mechanism for the ubiquitin-like protein NEDD8. (stjude.org)
Target proteins3
- Protein S-acylation is the reversible addition of fatty acids to the cysteine residues of target proteins. (frontiersin.org)
- Protein lipidation is the co-translational or post-translational covalent addition of a variety of lipids to target proteins. (frontiersin.org)
- However, the identities of Vpr target proteins through which these biological effects are exerted are unknown. (elifesciences.org)
Ubiquitination1
- The BTB domain (also known as the POZ domain) is a versatile protein-protein interaction motif that participates in a wide range of cellular functions, including transcriptional regulation, cytoskeleton dynamics, ion channel assembly and gating, and targeting proteins for ubiquitination. (biomedcentral.com)
Regulation3
- A family of structurally related proteins that were originally discovered for their role in cell-cycle regulation in CAENORHABDITIS ELEGANS. (uchicago.edu)
- Dynamic regulation of macroautophagy by distinctive ubiquitin-like proteins. (stjude.org)
- In this review, we will explore the recent findings on protein S-acylation, the enzymatic regulation of this process, and discuss examples of dynamic S-acylation. (frontiersin.org)
CUL31
- Since cullin-3 is produced in nerve cells throughout the brain, it is likely that the protein's dysfunction damages nerve cells, causing many of the signs and symptoms of CUL3 -related neurodevelopmental disorder. (medlineplus.gov)
Substrate5
- 15. Understanding cullin-RING E3 biology through proteomics-based substrate identification. (nih.gov)
- Although they have conserved catalytic cores, DHHC enzymes vary in their protein substrate selection, lipid substrate preference, and regulatory mechanisms. (frontiersin.org)
- In general, prenylation is an enzymatically mediated multi-step process that adds hydrophobic prenyl moieties to the C-terminal cysteines of substrate proteins. (frontiersin.org)
- For the rest of this review, we specifically discuss S-acylation, the covalent linkage of various fatty acids (14-20 carbons) via a thioester bond to the cysteine residues of substrate proteins. (frontiersin.org)
- The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. (nih.gov)
Pathways2
- 1. Alpha 1-Antitrypsin Deficiency: A Disorder of Proteostasis-Mediated Protein Folding and Trafficking Pathways. (nih.gov)
- We believe that determining the mechanisms by which enzymes transfer Ubls will be of broad importance, much like studies of protein kinases have influenced our knowledge of signaling pathways and their roles in diseases. (stjude.org)
Peptides1
- Studying short peptides allows bypassing the complexity of working with full-length proteins and may provide important information relative to critical segments of amyloid proteins. (deepdyve.com)
Aggregation3
- 4. Proteostasis impairment in protein-misfolding and -aggregation diseases. (nih.gov)
- 16. Protein Misfolding and Aggregation: The Relatedness between Parkinson's Disease and Hepatic Endoplasmic Reticulum Storage Disorders. (nih.gov)
- Alzheimer's, Parkinson's, type II diabetes, and Creutzfeldt-Jakob's diseases are part of a wider family encompassing more than 50 human pathologies related to aggregation of proteins. (deepdyve.com)
Arabidopsis1
- There are numerous lineage-specific expansions of BTB proteins, as seen by the relatively large number of BTB-ZF and BBK proteins in vertebrates, MATH-BTB proteins in Caenorhabditis elegans , and BTB-NPH3 proteins in Arabidopsis thaliana . (biomedcentral.com)
Abstract1
- ABSTRACT: The [URE3] prion is a self-propagating amyloid form of the Ure2 protein of Saccharomyces cere V isiae . (nih.gov)
Descriptor1
- Cullin Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
Sequences3
- We use the Structural Classification of Proteins (SCOP) database terminology of 'fold' to describe the set of BTB sequences that are known or predicted to share a secondary structure arrangement and topology, and the term 'family' to describe more highly related sequences that are likely to be functionally similar [ 21 ]. (biomedcentral.com)
- To solve this problem, several research groups have chosen to focus on short fragments of amyloid proteins, sequences that have been found to be of great importance for the amyloid formation process. (deepdyve.com)
- To learn about the virus-fighting potential of human ERVs, a research team led by Dr. Cedric Feschotte of Cornell University scanned the human genome for sequences that might code for receptor-binding portions of envelope proteins. (nih.gov)
Genes3
- McFadden's team has got around this by deleting six protein-coding genes from the BCG vaccine strain. (newscientist.com)
- The nonchromosomal genes [URE3] and [PSI] of Sac- activation activity of the C-terminal domain alone ( 19 ), charomyces cere V isiae are infectious proteins (prions) of apparently by recruiting Gln3p ( 20 ). (nih.gov)
- Recent advances in high-throughput technologies in genomics, proteomics, and metabolomics, in combination with robust bioinformatics enable researchers to rapidly survey thousands of differentially expressed genes, proteins, or metabolites simultaneously, thus employing an integrative approach to identify unique biomarker signatures. (nih.gov)
Bind2
- The function of the Suppressyn protein is poorly understood, but it is known to bind to a cell-surface receptor called ASCT2. (nih.gov)
- For her senior project, she is studying innovative ways to decontaminate water using naturally occurring proteins that bind contaminants and make them easier to remove from water. (villanova.edu)
Skp13
- In contrast, the Skp1 and ElonginC proteins consist almost exclusively of the core BTB fold. (biomedcentral.com)
- Thus, the BTB domain in BTB-zinc finger (ZF), Skp1, ElonginC and voltage-gated potassium channel T1 (T1-Kv) proteins all contain the BTB fold, even though some of these differ in their peripheral secondary structure elements and are involved in different types of protein-protein associations. (biomedcentral.com)
- In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. (nih.gov)
Amyloid3
- Understanding amyloid fibril formation using protein fragments: structural investigations via. (deepdyve.com)
- Auger, Michèle 2018-05-31 00:00:00 It is well established that amyloid proteins play a primary role in neurodegenerative diseases. (deepdyve.com)
- Ure2p1-89) with the C-terminal fragment (Ure2p95-354), but systems is based on the formation of a self-propagating might be an interaction of two parts of the C-terminal amyloid form of the respective protein ( 3 - 9 , reviewed in domain. (nih.gov)
Homeostasis1
- 7. [Alpha-1 antitrypsin deficiency: A model of alteration of protein homeostasis or proteostasis]. (nih.gov)
Eukaryotic1
- Post-translational covalent attachment of Ubls to protein targets is a primary eukaryotic regulatory mechanism. (stjude.org)
Regulates1
- By breaking down proteins at the right time, this system regulates the timing of critical cell activities, such as cell growth and division. (medlineplus.gov)
20201
- Elizabeth Cullen has been awarded a Fulbright U.S. Student Grant for 2019-2020. (villanova.edu)
Interactions4
- The fold has adapted to several different modes of self-association and interactions with non-BTB proteins. (biomedcentral.com)
- As a result, the BTB fold is a versatile scaffold that participates in a variety of types of family-specific protein-protein interactions. (biomedcentral.com)
- Given the range of functions, structures and interactions mediated by BTB domains, we undertook a survey of the abundance, protein architecture, conservation and structure of this fold. (biomedcentral.com)
- However, different Ubls alter the functions of their targets in different ways, such as by changing the target's subcellular localization, enzymatic activity, or interactions with other proteins or DNA. (stjude.org)
Zinc1
- The BTB domain is typically found as a single copy in proteins that contain only one or two other types of domain, and this defines the BTB-zinc finger (BTB-ZF), BTB-BACK-kelch (BBK), voltage-gated potassium channel T1 (T1-Kv), MATH-BTB, BTB-NPH3 and BTB-BACK-PHR (BBP) families of proteins, among others. (biomedcentral.com)
Neurodegenerative1
- 5. Underlying mechanisms and chemical/biochemical therapeutic approaches to ameliorate protein misfolding neurodegenerative diseases. (nih.gov)
Post-translational1
- The Schulman lab studies the structural basis for post-translational modification by ubiquitin and ubiquitin-like proteins (Ubls). (stjude.org)
Aspects of protein1
- 9. In vivo aspects of protein folding and quality control. (nih.gov)
Prion2
Chromosome1
- We show that a chromosome periphery protein, CCDC137/cPERP-B, is targeted for depletion by HIV-1 Vpr, in a cullin4A-DDB1-DCAF1 dependent manner. (elifesciences.org)
Mice2
Progression1
- Cullin-3 protein expression levels correlate with breast cancer progression. (karmanos.org)
Functional1
- In most of these functional classes, the BTB domain acts as a protein-protein interaction module that is able to both self-associate and interact with non-BTB proteins. (biomedcentral.com)
Mutant2
- We were looking at proteins that accumulate in the mutant brain and found a protein called Plastin 3. (ist.ac.at)
- When infected cells carried this mutant protein, they kept on dividing as normal. (elifesciences.org)
Role6
- The cullin-3 protein plays a role in the ubiquitin-proteasome system, which breaks down (degrades) unwanted proteins inside cells. (medlineplus.gov)
- 8. Hepatocyte proteomes reveal the role of protein disulfide isomerase 4 in alpha 1-antitrypsin deficiency. (nih.gov)
- The answer lies in the important role Cullin 3 plays at the end of life of proteins. (ist.ac.at)
- It turned out that the protein Plastin 3, which was previously unknown in the context of neuronal cell migration, actually plays an important role in this process. (ist.ac.at)
- Amongst these proteins, the role of Viral Protein R (Vpr for short) has been particularly enigmatic. (elifesciences.org)
- To investigate the role of CCDC137, Zhang and Bieniasz decreased the levels of the protein in human cells. (elifesciences.org)
Molecule1
- 19. Protein misfolding in disease and small molecule therapies. (nih.gov)
Composition1
- To find out, which proteins are misregulated when Cullin 3 is defective, Morandell and Schwarz systematically analyzed the protein composition of the mouse brain. (ist.ac.at)
Viral3
- Researchers found that stretches of viral DNA long embedded in the human genome can produce proteins that help block infection by viruses. (nih.gov)
- Further identification and study of these protective virus-based proteins could provide new insights for fighting viral infections. (nih.gov)
- Among the HIV-1 accessory proteins, the function of ~14 kDa Viral Protein R (Vpr) remains the most enigmatic. (elifesciences.org)
Interact1
- To investigate, Zhang and Bieniasz conducted a series of experiments to spot the proteins that interact with Vpr in human cells. (elifesciences.org)
Structural1
- SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
Cattle1
- If these six proteins are injected into the skin of cattle vaccinated with the new strain, there will be no response, but in infected cattle there will be a response. (newscientist.com)
Function1
- As a result, unnecessary proteins build up and interfere with the normal function of cells. (medlineplus.gov)
Attachment1
- Protein O -cholesterylation is the attachment of a sterol group to the C-terminus of a protein. (frontiersin.org)
Complex1
- A negative feedback loop mediated by the Bcl6-cullin 3 complex limits Tfh cell differentiation. (uchicago.edu)
Cell2
- The GPI anchor attaches proteins to the exterior surface of the cell through a glycolipid containing phosphatidylinositol. (frontiersin.org)
- While accessory proteins are often dispensable for replication in immortalized cell lines, they are important in a physiological context and typically act by removing or displacing molecules that are deleterious to virus replication. (elifesciences.org)
