Cryptosporidium parvum
Cryptosporidium
Cryptosporidiosis
Oocysts
Coccidia
Giardia
Feces
RNA, Ribosomal, 18S
Giardiasis
Giardia lamblia
RNA, Protozoan
Protozoan Infections
Water Supply
Sporozoites
Cattle
Diarrhea
Cattle Diseases
Apicomplexa
Water
Water Purification
Propionibacterium acnes
Antigens, Protozoan
Disinfection
Polymerase Chain Reaction
Fluorescent Antibody Technique, Direct
Immunomagnetic Separation
Amebicides
Intestinal Diseases, Parasitic
Host-Parasite Interactions
Water Microbiology
Polymorphism, Restriction Fragment Length
IMP Dehydrogenase
Isospora
Molecular Sequence Data
Food Parasitology
Zoonoses
Cryptosporidium parvum sporozoite pellicle antigen recognized by a neutralizing monoclonal antibody is a beta-mannosylated glycolipid. (1/679)
The protozoan parasite Cryptosporidium parvum is an important cause of diarrhea in humans, calves, and other mammals worldwide. No approved vaccines or parasite-specific drugs are currently available for the control of cryptosporidiosis. To effectively immunize against C. parvum, identification and characterization of protective antigens are required. We previously identified CPS-500, a conserved, neutralization-sensitive antigen of C. parvum sporozoites and merozoites defined by monoclonal antibody 18.44. In the present study, the biochemical characteristics and subcellular location of CPS-500 were determined. CPS-500 was chloroform extractable and eluted with acetone and methanol in silicic acid chromatography, consistent with being a polar glycolipid. Following chloroform extraction and silicic acid chromatography, CPS-500 was isolated by high-pressure liquid chromatography for glycosyl analysis, which indicated the presence of mannose and inositol. To identify which component of CPS-500 comprised the neutralization-sensitive epitope recognized by 18.44, the ability of the monoclonal antibody to bind CPS-500 treated with proteases, or with alpha- or beta-glycosidases, was determined. Monoclonal antibody 18.44 did not bind antigen treated with beta-D-mannosidase but did bind antigen treated with alpha-D-mannosidase, other alpha- or beta-glycosidases, or a panel of proteases. These data indicated that the target epitope was dependent on terminal beta-D-mannopyranosyl residues. By immunoelectron microscopy, 18.44 binding was localized to the pellicle and an intracytoplasmic tubulovesicular network in sporozoites. Monoclonal antibody 18.44 also bound to antigen deposited and released onto substrate over the course travelled by gliding sporozoites and merozoites. Surface localization, adhesion and release during locomotion, and neutralization sensitivity suggest that CPS-500 may be involved in motility and invasion processes of the infective zoite stages. (+info)Cytokine profile induced by Cryptosporidium antigen in peripheral blood mononuclear cells from immunocompetent and immunosuppressed persons with cryptosporidiosis. (2/679)
The proliferative response of peripheral blood mononuclear cells (PBMC) to a crude extract from Cryptosporidium parvum (CCE) was studied in persons who acquired cryptosporidiosis in the same outbreak (15 immunocompetent subjects with prior cryptosporidiosis and 22 human immunodeficiency virus [HIV]-positive persons with various levels of immunosuppression and active cryptosporidiosis) and in individual patients (8 HIV-positive patients with active cryptosporidiosis and 15 HIV-positive persons without history of cryptosporidiosis). PBMC from HIV-positive persons showed less proliferation to CCE and mitogens than did PBMC from immunocompetent subjects with prior cryptosporidiosis, independent of CD4 cell count. In immunocompetent subjects, cytokine gene expression was consistent with cytokine production, whereas in HIV-positive subjects it was not. The production of interferon-gamma in CCE-stimulated PBMC from both immunocompetent and HIV-positive subjects with cryptosporidiosis and the lack of interferon-gamma in CCE-stimulated PBMC from HIV-positive subjects without cryptosporidiosis indicate that C. parvum mainly induces a Th1 response. (+info)Characterization of an immunogenic glycocalyx on the surfaces of Cryptosporidium parvum oocysts and sporozoites. (3/679)
Ruthenium red staining of Cryptosporidium parvum oocysts revealed the presence of a carbohydrate matrix on their outer bilayers that is characteristic of a glycocalyx. Surface labeling of intact oocysts identified material of high molecular weight (>10(6)) that reacted positively with sera from cryptosporidium-infected patients and with immunoglobulin A monoclonal antibodies. (+info)beta-tubulin mRNA as a marker of Cryptosporidium parvum oocyst viability. (4/679)
Determining the viability of waterborne Cryptosporidium parvum oocysts remains a technical challenge. rRNA and mRNA were evaluated in a reverse transcription (RT)-PCR assay as potential markers of oocyst viability. The rationale for this approach is the rapid turnover and postmortem decay of cellular RNA. The beta-tubulin mRNA and an anonymous mRNA transcript were chosen as potential markers because they are the only mRNA species in C. parvum known to possess introns. This feature facilitated the distinction between genuine RT-PCR products and PCR products originating from copurifying DNA. Prolonged incubation at room temperature of initially viable oocysts resulted in a gradual decrease in mRNA levels, which correlated with the loss of oocyst infectivity to neonatal mice. In contrast, oocysts stored at 4 degrees C for over 39 weeks maintained their infectivity and displayed no decrease in the level of beta-tubulin RT-PCR product. The postmortem decay of two mRNA species demonstrates that RT-PCR analysis can provide information on the viability of C. parvum oocysts. The methodological similarity between PCR detection and RT-PCR viability analysis could facilitate the development of a combined detection and viability assay. (+info)The Cryptosporidium "mouse" genotype is conserved across geographic areas. (5/679)
A 298-bp region of the Cryptosporidium parvum 18S rRNA gene and a 390-bp region of the acetyl coenzyme A synthetase gene were sequenced for a range of Cryptosporidium isolates from wild house mice (Mus domesticus), a bat (Myotus adversus), and cattle from different geographical areas. Previous research has identified a distinct genotype, referred to as the "mouse"-derived Cryptosporidium genotype, common to isolates from Australian mice. Comparison of a wider range of Australian mouse isolates with United Kingdom and Spanish isolates from mice and cattle and also an Australian bat-derived Cryptosporidium isolate revealed that the "mouse" genotype is conserved across geographic areas. Mice are also susceptible to infection with the "cattle" Cryptosporidium genotype, which has important implications for their role as reservoirs of infection for humans and domestic animals. (+info)Detection by enzyme immunoassay of serum immunoglobulin G antibodies that recognize specific Cryptosporidium parvum antigens. (6/679)
Human infection with Cryptosporidium parvum usually elicits characteristic immunoglobulin G (IgG), IgA, and IgM antibody responses against two sporozoite surface antigens with apparent molecular masses of approximately 27 and 17 kDa. We have determined that these two antigens are actually complex families of related antigens. We have developed two new enzyme-linked immunosorbent assays (ELISAs) for the detection and quantitation of serum IgG antibodies against both antigens. The assays utilize a recombinant form of the 27-kDa antigen and a partially purified native fraction isolated from sonicated whole oocysts that contains 17-kDa antigen. An immunoblot assay previously developed in our laboratory served as the reference, or "gold standard," seroassay for the assessment of the new ELISAs. Positive responses with the recombinant-27-kDa-antigen ELISA were correlated with the immunoblot results for the 27-kDa antigen, with a sensitivity and specificity of 90 and 92%, respectively. Similarly, positive responses with the partially purified native-17-kDa-antigen ELISA correlated with the immunoblot results for the 17-kDa antigen, with a sensitivity and specificity of 90 and 94%, respectively. For both ELISAs the median IgG antibody levels for serum sets collected during outbreaks of waterborne C. parvum infection were at least 2.5-fold higher than the levels determined for a nonoutbreak set. Using the immunoblot as the "gold standard," the new ELISAs were more specific and, in the case of the 27-kDa-antigen ELISA, more sensitive than the crude oocyst antigen ELISA currently in use. These assays will be useful in future epidemiologic studies. (+info)Use of a sentinel system for field measurements of Cryptosporidium parvum oocyst inactivation in soil and animal waste. (7/679)
A small-volume sentinel chamber was developed to assess the effects of environmental stresses on survival of sucrose-Percoll-purified Cryptosporidium parvum oocysts in soil and animal wastes. Chambers were tested for their ability to equilibrate with external chemical and moisture conditions. Sentinel oocysts were then exposed to stresses of the external environment that affected their viability (potential infectivity), as indicated by results of a dye permeability assay. Preliminary laboratory experiments indicated that temperatures between 35 and 50 degrees C and decreases in soil water potential (-0.003 to -3.20 MPa) increased oocyst inactivation rates. The effects of two common animal waste management practices on oocyst survival were investigated on three dairy farms in Delaware County, N.Y., within the New York City watershed: (i) piling wastes from dairy youngstock (including neonatal calves) and (ii) spreading wastes as a soil amendment on an agricultural field. Sentinel containers filled with air-dried and sieved (2-mm mesh) youngstock waste or field soil were wetted and inoculated with 2 million oocysts in an aqueous suspension and then placed in waste piles on two different farms and in soil within a cropped field on one farm. Controls consisted of purified oocysts in either phosphate-buffered saline or distilled water contained in sealed microcentrifuge tubes. Two microdata loggers recorded the ambient temperature at each field site. Sentinel experiments were conducted during the fall and winter (1996 to 1997) and winter (1998). Sentinel containers and controls were removed at 2- to 4-week intervals, and oocysts were extracted and tested by the dye permeability assay. The proportions of potentially infective oocysts exposed to the soil and waste pile material decreased more rapidly than their counterpart controls exposed to buffer or water, indicating that factors other than temperature affected oocyst inactivation in the waste piles and soil. The effect of soil freeze-thaw cycles was evident in the large proportion of empty sentinel oocysts. The potentially infective sentinel oocysts were reduced to <1% while the proportions in controls did not decrease below 50% potentially infective during the first field experiment. Microscopic observations of empty oocyst fragments indicated that abrasive effects of soil particles were a factor in oocyst inactivation. A similar pattern was observed in a second field experiment at the same site. (+info)CpABC, a Cryptosporidium parvum ATP-binding cassette protein at the host-parasite boundary in intracellular stages. (8/679)
The intracellular parasite Cryptosporidium parvum develops inside a vacuole at the apex of its epithelial host cell. The developing parasite is separated from the host cell cytoplasm by a zone of attachment that consists of an extensively folded membranous structure known as the feeder organelle. It has been proposed that the feeder organelle is the site of regulation of transport of nutrients and drugs into the parasite. In this report, we localize an approximately 200-kDa integral membrane protein, CpABC, from Cryptosporidium parvum to the host-parasite boundary, possibly the feeder organelle. The predicted amino acid sequence of CpABC has significant structural similarity with the cystic fibrosis conductance regulator and the multidrug resistance protein subfamily of ATP-binding cassette proteins. This is an example of a parasite-encoded transport protein localized at the parasite-host interface of an intracellular protozoan. (+info)Cryptosporidiosis is a parasitic infection caused by the Cryptosporidium parasite. It is a common cause of diarrhea, especially in children and people with weakened immune systems. The parasite is transmitted through contaminated water or food, or by contact with infected feces. Symptoms of cryptosporidiosis include watery diarrhea, stomach cramps, nausea, and vomiting. In severe cases, it can lead to dehydration and malnutrition. Cryptosporidiosis is typically diagnosed through stool tests and can be treated with antiparasitic medications. Prevention measures include proper hand hygiene, safe food and water handling, and avoiding contact with feces.
DNA, Protozoan refers to the genetic material of protozoans, which are single-celled organisms that belong to the kingdom Protista. Protozoans are a diverse group of organisms that can be found in a variety of environments, including soil, water, and the human body. Protozoans have their own unique DNA, which contains the genetic information necessary for their growth, development, and reproduction. This DNA is organized into chromosomes, which are structures that contain the genetic material of an organism. In the medical field, knowledge of the DNA of protozoans is important for understanding the biology of these organisms and for developing treatments for infections caused by protozoans. For example, the DNA of the protozoan Plasmodium, which causes malaria, has been extensively studied in order to develop drugs and vaccines to treat and prevent this disease.
RNA, Ribosomal, 18S is a type of ribosomal RNA (rRNA) that is a component of the small ribosomal subunit in eukaryotic cells. It is responsible for binding to the mRNA (messenger RNA) and facilitating the process of protein synthesis by the ribosome. The 18S rRNA is one of the three main types of rRNA found in eukaryotic cells, along with 5.8S rRNA and 28S rRNA. Abnormalities in the expression or function of 18S rRNA have been associated with various diseases, including cancer and neurological disorders.
Giardiasis is a common parasitic infection caused by the protozoan parasite Giardia lamblia. It is transmitted through contaminated water or food, or by direct contact with an infected person or animal. The parasite infects the small intestine and can cause a range of symptoms, including diarrhea, abdominal cramps, bloating, gas, nausea, and vomiting. In some cases, giardiasis can lead to malnutrition, dehydration, and weight loss. Treatment typically involves the use of antiparasitic medications, although in some cases, the infection may resolve on its own. Prevention measures include practicing good hygiene, avoiding contaminated water and food, and properly cooking and handling food.
RNA, Protozoan refers to the ribonucleic acid (RNA) molecules that are found in protozoan organisms. Protozoa are a diverse group of single-celled eukaryotic organisms that include many parasites, such as Plasmodium (which causes malaria) and Trypanosoma (which causes African sleeping sickness). RNA is a nucleic acid that plays a crucial role in the expression of genetic information in cells. It is involved in the process of transcription, where the genetic information stored in DNA is copied into RNA, and in the process of translation, where the RNA is used to synthesize proteins. Protozoan RNA can be studied to understand the biology and pathogenesis of these organisms, as well as to develop new treatments for the diseases they cause. For example, researchers have used RNA interference (RNAi) to silence specific genes in protozoan parasites, which can help to block their ability to infect and cause disease in humans and animals.
Protozoan infections are a group of diseases caused by single-celled organisms called protozoa. Protozoa are microscopic organisms that can be found in various environments, including soil, water, and the human body. They can cause a wide range of infections in humans, including gastrointestinal, respiratory, and bloodstream infections. Protozoan infections can be transmitted through various routes, including contaminated food and water, sexual contact, and contact with infected animals. Some common examples of protozoan infections include giardiasis, cryptosporidiosis, toxoplasmosis, and malaria. The symptoms of protozoan infections can vary depending on the type of infection and the severity of the illness. Some common symptoms include diarrhea, abdominal pain, nausea, vomiting, fever, headache, and fatigue. In severe cases, protozoan infections can lead to complications such as organ damage, anemia, and death. Treatment for protozoan infections typically involves the use of antiprotozoal medications, which are designed to kill or inhibit the growth of the protozoa. In some cases, supportive care such as fluid replacement and electrolyte replacement may also be necessary. Prevention of protozoan infections involves practicing good hygiene, avoiding contaminated food and water, and taking precautions when traveling to areas where these infections are common.
Paromomycin is an antibiotic medication that is used to treat a variety of bacterial and parasitic infections. It is a member of the aminoglycoside class of antibiotics, which work by binding to and inhibiting the function of bacterial ribosomes, preventing the production of essential proteins and ultimately leading to bacterial cell death. Paromomycin is primarily used to treat infections caused by the parasite Trypanosoma brucei, which is responsible for African sleeping sickness. It is also used to treat infections caused by other parasites, such as Leishmania donovani, which can cause leishmaniasis, and Entamoeba histolytica, which can cause amoebic dysentery. In addition to its use in treating parasitic infections, paromomycin is also used to treat certain bacterial infections, such as typhoid fever and cholera. It is typically administered intravenously or orally, depending on the specific infection being treated and the severity of the illness. It is important to note that paromomycin can cause serious side effects, including hearing loss, kidney damage, and allergic reactions. It should only be used under the supervision of a healthcare professional, and patients should be closely monitored for any signs of adverse reactions while taking the medication.
Antibodies, Protozoan refers to a type of antibody that is produced by the immune system in response to infections caused by protozoan parasites. Protozoan parasites are single-celled organisms that can cause a variety of diseases in humans and animals, including malaria, sleeping sickness, and giardiasis. Antibodies are proteins that are produced by immune cells called B cells. They are designed to recognize and bind to specific molecules on the surface of pathogens, such as viruses, bacteria, and parasites. When an antibody binds to a pathogen, it can help to neutralize the pathogen or mark it for destruction by other immune cells. Antibodies, Protozoan are specific to the antigens found on the surface of protozoan parasites. They are produced in response to an infection with a specific protozoan parasite and can help to protect the body against future infections with that parasite.
Diarrhea is a medical condition characterized by the passage of loose, watery stools more than three times a day. It can be acute, meaning it lasts for a short period of time, or chronic, meaning it persists for more than four weeks. Diarrhea can be caused by a variety of factors, including infections, food poisoning, medications, underlying medical conditions, and stress. It can also be a symptom of other medical conditions, such as inflammatory bowel disease, celiac disease, and irritable bowel syndrome. Diarrhea can cause dehydration, electrolyte imbalances, and malnutrition if it persists for an extended period of time. Treatment for diarrhea depends on the underlying cause and may include medications, dietary changes, and fluid replacement therapy. In severe cases, hospitalization may be necessary.
Protozoan proteins are proteins that are produced by protozoa, which are single-celled organisms that belong to the kingdom Protista. Protozoa are found in a wide range of environments, including soil, water, and the bodies of animals and humans. Protozoan proteins can be of interest in the medical field because some protozoa are pathogenic, meaning they can cause disease in humans and other animals. For example, the protozoan parasite Trypanosoma brucei, which causes African sleeping sickness, produces a number of proteins that are important for its survival and replication within the host organism. Protozoan proteins can also be studied as potential targets for the development of new drugs to treat protozoan infections. For example, researchers are exploring the use of antibodies that target specific protozoan proteins to prevent or treat diseases caused by these organisms. In addition to their potential medical applications, protozoan proteins are also of interest to researchers studying the evolution and biology of these organisms. By studying the proteins produced by protozoa, scientists can gain insights into the genetic and biochemical mechanisms that underlie the biology of these organisms.
Cattle diseases refer to any illness or condition that affects cattle, which are domesticated animals commonly raised for meat, milk, and other products. These diseases can be caused by a variety of factors, including bacteria, viruses, fungi, parasites, and environmental conditions. In the medical field, cattle diseases are typically studied and treated by veterinarians who specialize in animal health. Some common cattle diseases include bovine respiratory disease (BRD), Johne's disease, foot-and-mouth disease, and mastitis. These diseases can have significant economic impacts on farmers and the cattle industry, as they can lead to decreased productivity, increased mortality rates, and the need for costly treatments. To prevent and control cattle diseases, veterinarians and farmers may use a variety of strategies, including vaccination, proper nutrition and hygiene, and the use of antibiotics and other medications when necessary. Additionally, monitoring and surveillance efforts are often implemented to detect and respond to outbreaks of new or emerging diseases.
In the medical field, water is a vital substance that is essential for the proper functioning of the human body. It is a clear, odorless, tasteless liquid that makes up the majority of the body's fluids, including blood, lymph, and interstitial fluid. Water plays a crucial role in maintaining the body's temperature, transporting nutrients and oxygen to cells, removing waste products, and lubricating joints. It also helps to regulate blood pressure and prevent dehydration, which can lead to a range of health problems. In medical settings, water is often used as a means of hydration therapy for patients who are dehydrated or have fluid imbalances. It may also be used as a diluent for medications or as a component of intravenous fluids. Overall, water is an essential component of human health and plays a critical role in maintaining the body's normal functions.
Intestinal diseases caused by parasites are a group of conditions that affect the digestive system and are caused by the presence of parasites in the intestines. These parasites can be protozoa, helminths, or other microorganisms that live in the digestive tract and cause damage to the lining of the intestine, leading to symptoms such as abdominal pain, diarrhea, nausea, vomiting, and weight loss. Some common examples of parasitic intestinal diseases include: 1. Ascariasis: caused by the roundworm Ascaris lumbricoides, which can cause abdominal pain, diarrhea, and coughing up worms. 2. Giardiasis: caused by the protozoan Giardia lamblia, which can cause diarrhea, abdominal cramps, and bloating. 3. Hookworm infection: caused by the hookworms Necator americanus and Ancylostoma duodenale, which can cause anemia, abdominal pain, and weight loss. 4. Trichomoniasis: caused by the protozoan Trichomonas vaginalis, which can cause diarrhea, abdominal pain, and nausea. 5. Schistosomiasis: caused by parasitic flatworms called schistosomes, which can cause abdominal pain, diarrhea, and blood in the stool. Treatment for parasitic intestinal diseases typically involves the use of antiparasitic medications, although in some cases, surgery may be necessary. Prevention measures include practicing good hygiene, avoiding contaminated food and water, and using insect repellent to prevent mosquito bites.
IMP dehydrogenase (Inosine 5'-Monophosphate Dehydrogenase) is an enzyme that plays a crucial role in the metabolism of purines, which are essential building blocks of nucleic acids such as DNA and RNA. The enzyme catalyzes the conversion of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP), which is a precursor for the synthesis of guanine nucleotides. IMP dehydrogenase is involved in the regulation of purine biosynthesis and is a key target for the treatment of certain diseases, including cancer. In cancer cells, the enzyme is often overexpressed, leading to an increased production of guanine nucleotides and promoting cell proliferation and survival. Therefore, inhibitors of IMP dehydrogenase have been developed as potential cancer therapeutics. In addition to its role in purine metabolism, IMP dehydrogenase has also been implicated in the regulation of other cellular processes, such as cell differentiation and apoptosis.
In the medical field, sewage refers to the waste water that is generated from households, industries, and commercial establishments. It contains a mixture of water, solid waste, and various contaminants such as bacteria, viruses, parasites, and chemicals. Sewage is considered a potential source of disease transmission and can pose a risk to public health if not properly treated and disposed of. Therefore, the collection, treatment, and disposal of sewage are important public health measures to prevent the spread of waterborne diseases.
Zoonoses are infectious diseases that are transmitted from animals to humans. These diseases can be caused by bacteria, viruses, parasites, or fungi, and can be transmitted through direct contact with animals, their bodily fluids, or their feces, or through the bites of infected insects or ticks. Examples of zoonoses include rabies, Lyme disease, brucellosis, and salmonellosis. Zoonoses can be a significant public health concern, as they can spread rapidly and cause serious illness or even death in humans. They can also have a significant economic impact, as they can affect livestock and wildlife populations, and can lead to the closure of farms or the destruction of animals to prevent the spread of disease.
Chlorine compounds are chemical compounds that contain chlorine as an element. In the medical field, chlorine compounds are commonly used as disinfectants, antiseptics, and antifungals. They are also used in the production of various pharmaceuticals and medical devices. One of the most well-known chlorine compounds used in medicine is hypochlorous acid (HOCl), which is produced by the immune system as a natural defense against bacteria, viruses, and fungi. It is also used as a disinfectant in hospitals and other healthcare facilities. Other chlorine compounds used in medicine include chlorhexidine, which is used as an antiseptic in mouthwashes and skin cleansers, and chloramphenicol, which is used as an antibiotic to treat bacterial infections. However, it is important to note that some chlorine compounds can be toxic and can cause harm if not used properly. Therefore, it is essential to follow proper safety protocols when handling and using chlorine compounds in the medical field.
Cryptosporidium parvum
Happy mapping
Gene density
Mitochondrion
NIAID ChemDB
Paternal mtDNA transmission
Intracellular parasite
Carcinogenic parasite
Sewage
Herbert L. DuPont
Cryptosporidium
List of sequenced eukaryotic genomes
List of sequenced protist genomes
Pyruvate dehydrogenase (NADP+)
Bacterial adhesion in aquatic system
Cryspovirus
Catskill-Delaware Water Ultraviolet Disinfection Facility
Protein O-GlcNAc transferase
Phytaspase
Cryptosporidium hominis
Nicotinamide adenine dinucleotide
Opportunistic infection
FGD4
Acid-fastness
Cryptosporidiosis
Cryptosporidium muris
Nonpoint source water pollution regulations in the United States
Host adaptation
FAM76A
Limosilactobacillus reuteri
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Giardia4
- Water samples from the sources and intermediaries were analysed for thermotolerant faecal coliforms (FCs), Giardia lamblia, Cryptosporidium parvum and microsporidia, using immunofluorescent antibody detection for G. lamblia and C. parvum, and chromotrope-2 stain for microsporidia. (who.int)
- FCs), Giardia lamblia , Cryptosporidium parvum and microsporidia, using immunofluorescent antibody detection for G. lamblia and C. parvum , and chromotrope-2 stain for microsporidia. (who.int)
- Des échantillons d'eau à la source et au niveau des intermédiaires ont été analysés afin de détecter la présence de coliformes thermotolérants, de Giardia lamblia , de Cryptosporidium parvum et de microsporidies, à l'aide de la recherche des anticorps par immunofluorescence pour G. lamblia and C. parvum , et de la coloration au chromotrope 2R pour les microsporidies. (who.int)
- LifeStraw®, the original ultralight backpacking and travel-friendly "straw" water filter, makes contaminated water safe to drink and helps to prevent most waterborne diseases by removing bacteria (like E.coli), parasites (like Giardia and Cryptosporidium) and microplastics. (canadiangreatoutdoors.com)
Oocysts4
- The oocysts of C.parvum, an obligate intracellular parasite, cause infection when ingested orally. (bvsalud.org)
- For the study, 50 ml of unpasteurized cow's milk was contaminated with 5 X 105 C.parvum oocysts and portioned into 1.5 ml microcentrifuge tubes. (bvsalud.org)
- As a result, it was concluded that live C.parvum cysts in milk could be detected by PMA-qPCR method and live oocysts could be found in pasteurized milk. (bvsalud.org)
- Cryptosporidium muris oocysts found in human feces. (wikidoc.org)
Cryptosporidiosis2
- Cryptosporidium is a protozoan pathogen of the Phylum Apicomplexa and causes a diarrheal illness called cryptosporidiosis . (wikidoc.org)
- Cryptosporidiosis (Cryptosporidium spp. (cdc.gov)
Campylobacter1
- H7), Campylobacter jejuni, and Cryptosporidium parvum have been documented to occur in the dairy farm environment. (cdc.gov)
Pathogens1
- One of these pathogens is Cryptosporidium parvum. (bvsalud.org)
Diarrhea3
- Cryptosporidium is a protozoan that is often associated with diarrhea in humans. (wikidoc.org)
- 29- Diarrhea caused by Cryptosporidium parvum in Kut, Iraq using different methods. (edu.iq)
- An illness caused by the protozoan Cryptosporidium parvum and characterized by diarrhea, abdominal cramps, loss of appetite, low-grade fever, nausea, and vomiting. (cdc.gov)
Lamblia1
- Although FCs, as well as G. lamblia , C. parvum and were estimated to be caused by inad- FCs may be pathogenic, most often microsporidia. (who.int)
Isolates2
- We aimed at characterizing the genome of CSpV1 from France and used sequence analysis from Cryptosporidium isolates to explore whether CSpV1 genome diversity varies over time, with geographical sampling location, C. parvum genetic diversity, or ruminant host species. (bvsalud.org)
- Subtyping analysis of the C. parvum isolates revealed the presence of two zoonotic subtype families IIa and IId. (bvsalud.org)
Genome2
- Full-length genome sequences have been obtained from C. parvum from Iowa (Iowa), Kansas (KSU) and China. (bvsalud.org)
- The genome of Cryptosporidium parvum was sequenced in 2004 and was found to be unusual amongst Eukaryotes in that the mitochondria seem not to contain DNA [1] . (wikidoc.org)
Diagnosis1
- 26- Comparison among Modified Acid-Fast Stain and Some Immunological Methods in Diagnosis of Cryptosporidium parvum in Kut City. (edu.iq)
Humans1
- In humans, the main causes of disease are C. parvum and C. hominis (previously C. parvum genotype 1). (wikidoc.org)
Genus1
- Cryptosporidium parvum virus 1 (CSpV1), of the genus Cryspovirus, was first reported to be present in the cytoplasm of C. parvum in 1997. (bvsalud.org)
Filtration1
- Because of this resistance, water purification to eliminate Cryptosporidium generally relies upon coagulation followed by filtration or boiling. (wikidoc.org)
Species3
- Yet, there is no distinct clustering of CSpV1 per French department or ruminants, probably due to trade, and transmission of C. parvum among host species. (bvsalud.org)
- A number of species of Cryptosporidium infect mammals. (wikidoc.org)
- Several species of the Cryptosporidium family (C. serpentes and others) are involved, and outside of geckos it has been found in monitor lizards, iguanas, tortoises as well as several snake species. (wikidoc.org)
Occur1
- With the proper concentrations and contact time, Cryptosporidium inactivation will occur with chlorine dioxide and ozone treatment. (wikidoc.org)
Illness1
- Product size at microsatellite locus ML1 with number of Cryptosporidium parvum case-patients who touched or handled farm animals before onset of illness. (cdc.gov)
Sequence1
- Sequence analysis of CSpV1 revealed that all CSpV1 from France, regardless of the subtype of C. parvum (IIaA15G2R1, IIaA17G2R1 and IIdA18G1R1) are more closely related to CSpV1 from Turkey, and cluster on a distinct branch from CSpV1 collected from C. parvum subtype IIaA15G2R1 from Asia and North America. (bvsalud.org)
Water2
- It also stimulated efforts to develop regulatory standards for Cryptosporidium in drinking water. (cdc.gov)
- Recently, it has been discovered that Cryptosporidium is sensitive to ultraviolet light and ozonation , and water treatments based on these sterilization methods are being developed. (wikidoc.org)
Host3
- We also found that samples collected on a given year or successive years in a given location are more likely to host the same subtype of C. parvum and the same CSpV1 strain. (bvsalud.org)
- Unlike Plasmodium , which transmits via a mosquito vector, Cryptosporidium does not utilize an insect vector and is capable of completing its life cycle within a single host, resulting in cyst stages which are excreted in feces and are capable of transmission to a new host. (wikidoc.org)
- Cryptosporidium has a spore phase ( oocyst ) and in this state can survive for lengthy periods outside a host and also can resist many common disinfectants , notably chlorine based disinfectants. (wikidoc.org)
Study1
- The aim of this study was to investigate the viability of C.parvum by propidium monoazide (PMA)-quantitative polymerase chain reaction (qPCR) method after heat treatment applied to contaminated raw cow's milk. (bvsalud.org)
Giardia lamblia4
- FCs), Giardia lamblia , Cryptosporidium parvum and microsporidia, using immunofluorescent antibody detection for G. lamblia and C. parvum , and chromotrope-2 stain for microsporidia. (who.int)
- Des échantillons d'eau à la source et au niveau des intermédiaires ont été analysés afin de détecter la présence de coliformes thermotolérants, de Giardia lamblia , de Cryptosporidium parvum et de microsporidies, à l'aide de la recherche des anticorps par immunofluorescence pour G. lamblia and C. parvum , et de la coloration au chromotrope 2R pour les microsporidies. (who.int)
- Water samples from the sources and intermediaries were analysed for thermotolerant faecal coliforms (FCs), Giardia lamblia, Cryptosporidium parvum and microsporidia, using immunofluorescent antibody detection for G. lamblia and C. parvum, and chromotrope-2 stain for microsporidia. (who.int)
- however, a wide spectrum of accompanying infectious microorganisms are also found in surface waters including Burkholderia pseudomallei , Salmonella, Cryptosporidium parvum , Giardia lamblia , in addition to parasitic worms including the Schistosoma type, and viruses such as norovirus [7]. (dysona.org)
Parasites1
- Stool specimens obtained from two of these persons were negative for bacterial pathogens and for ova and parasites but were not tested for C. parvum. (cdc.gov)
Infection2
- PRECLINICAL EVALUATION OF THERAPIES OF CRYPTOSPORIDIUM PARVUM NIH GUIDE, Volume 25, Number 10, March 29, 1996 RFP AVAILABLE: NIH-NIAID-DAIDS-97-05 P.T. 34 Keywords: Chemotherapy Infectious Diseases/Agents National Institute of Allergy and Infectious Diseases The NIAID, DAIDS, has a requirement to quantitatively evaluate therapeutic agents for efficacy against Cryptosporidium parvum infection in a standardized, validated immunocompromised mouse model test system. (nih.gov)
- The offeror should have available a primary mouse model of Cryptosporidium infection and an in vitro system (including relevant Cryptosporidium isolates) suitable for the evaluation of therapies with potential for treatment of Cryptosporidium infections in people with AIDS. (nih.gov)
Confirmation1
- Confirmation of the presence of Cryptosporidium in environmental samples is laborious, costly and often difficult. (who.int)
Surface2
- Editorial Note: Known modes of transmission of C. parvum include consumption of contaminated surface or ground water (1,2), exposure to contaminated recreational water (3), animal-to-person contact (2), and person-to-person contact (2). (cdc.gov)
- Labeling surface epitopes to identify Cryptosporidium life stages using a scanning electron microscopy-based immunogold approach. (nih.gov)
Treatment1
- Cryptosporidium and Giardia: treatment options and prospects for new drugs. (nih.gov)