Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Microscopy, Electron, Transmission
Urea Cycle Disorders, Inborn
Rare congenital metabolism disorders of the urea cycle. The disorders are due to mutations that result in complete (neonatal onset) or partial (childhood or adult onset) inactivity of an enzyme, involved in the urea cycle. Neonatal onset results in clinical features that include irritability, vomiting, lethargy, seizures, NEONATAL HYPOTONIA; RESPIRATORY ALKALOSIS; HYPERAMMONEMIA; coma, and death. Survivors of the neonatal onset and childhood/adult onset disorders share common risks for ENCEPHALOPATHIES, METABOLIC, INBORN; and RESPIRATORY ALKALOSIS due to HYPERAMMONEMIA.
Stable elementary particles having the smallest known negative charge, present in all elements; also called negatrons. Positively charged electrons are called positrons. The numbers, energies and arrangement of electrons around atomic nuclei determine the chemical identities of elements. Beams of electrons are called CATHODE RAYS.
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The branch of medicine concerned with the application of NANOTECHNOLOGY to the prevention and treatment of disease. It involves the monitoring, repair, construction, and control of human biological systems at the molecular level, using engineered nanodevices and NANOSTRUCTURES. (From Freitas Jr., Nanomedicine, vol 1, 1999).
Morphology of intraepithelial corpuscular nerve endings in the nasal respiratory mucosa of the dog. (1/114)Corpuscular nerve endings in the nasal respiratory mucosa of the dog were investigated by immunohistochemical staining specific for protein gene product 9.5 by light and electron microscopy. In the nasal respiratory mucosa, complex corpuscular endings, which displayed bulbous, laminar and varicose expansions, were distributed on the dorsal elevated part of the nasal septum and on the dorsal nasal concha. The endings were 300-500 microm long and 100-250 microm wide. Some axons gave rise to a single ending while others branched into 2 endings. Cryostat sections revealed that the corpuscular endings were located within the nasal respiratory epithelium. On electron microscopy, immunoreactive nerve terminals that contained organelles, including mitochondria and neurofilaments, were observed within the epithelial layer near the lumen of the nasal cavity. Some terminals contacted the goblet cell. Such terminal regions were covered by the cytoplasmic process of ciliated cells and were never exposed to the lumen of the nasal cavity. These nerve endings are probably activated by pressure changes. (+info)
Electron diffraction provides new information on human stratum corneum lipid organization studied in relation to depth and temperature. (2/114)The outermost layer of mammalian skin, the stratum corneum, provides the body with a barrier against transepidermal water loss and penetration of agents from outside. The lipid-rich extracellular matrix surrounding the corneocytes in the stratum corneum is mainly responsible for this barrier function. In this study (cryo-) electron diffraction was applied to obtain information about the local lateral lipid organization in the extracellular matrix in relation to depth in human stratum corneum. For this purpose, stratum corneum grid-strips were prepared from native skin in vivo and ex vivo. It was found that the lipid packing in samples prepared at room temperature is predominantly orthorhombic. In samples prepared at 32 degrees C the presence of a hexagonal packing is more pronounced in the outer layers of the stratum corneum. Gradually increasing the specimen temperature from 30 to 40 degrees C induced a further transition from an orthorhombic to a hexagonal sublattice. At 90 degrees C all lipids were present in a fluid phase. These results are in good agreement with previously reported wide angle X-ray diffraction and Fourier transformed infrared spectroscopy studies. We conclude that the lipids in human stratum corneum are highly ordered throughout the stratum corneum and that electron diffraction allows monitoring of the local lipid organization, which contributes to the understanding of stratum corneum barrier function. (+info)
Gene delivery to the myocardium by intrapericardial injection. (3/114)Several studies have demonstrated the feasibility of gene transfer into the heart muscle. However, all the available data also indicate that the extent of transfection remains limited. As an alternative method to intravascular administration, we have developed a novel strategy which uses the pericardial sac. When a replication-deficient adenovirus containing the cDNA encoding a bacterial beta-galactosidase is injected into the pericardial sac of adult Wistar rats the staining is exclusively restricted to the pericardial cell layers. However, injecting a mixture of collagenase and hyaluronidase together with the virus, leads to a large diffusion of the transgene activity, reaching up to 40% of the myocardium. Transgene expression is predominant in the left ventricle and the interventricular septum but limited in the right ventricle. In vivo echocardiographic measurements of the left ventricular diameters at end diastolic and end systolic times show no difference between virus- and sham-injected animals, thus indicating a good clinical tolerance to this strategy of virus delivery. The same protocol has been used with the same efficiency in mice, which leads us to propose injection into the pericardial sac as an effective and harmless method for gene transfer into the heart muscle. (+info)
Scanning electron microscopic detection of nuclear structures involved in DNA replication. (4/114)In order to evaluate at the ultrastructural level the three dimensional chromatin arrangement during interphase and particularly during the S phase, the immunogold detection of Bromodeoxyuridine (BrdU), as a marker of DNA synthesis, was performed in human HeLa, HL60, and in murine Friend leukemia cells (FLC). Field emission in lens scanning electron microscopy analysis of ultrathin cryosections revealed the presence of a regular three-dimensional network of fibers in dispersed chromatin. This spatial architecture was apparently constituted mainly of 10 nm filaments organized in loops of about 80-100 nm. Nodal points and the overlapping of such coils appeared as thicker structures of about 30 nm in diameter. Thin filaments of about 5 nm did not show a regular distribution. This three-dimensional fiber organization seemed quite constant in the dispersed chromatin of all the cell lines analyzed. The DNase treatment of the samples selectively removed the 10 nm class fibers, whereas the BrdU labeling confirmed the presence of newly synthesized DNA organized into chromatin units with a regular arrangement. These data suggest that the 10 nm chromatin fiber likely represents the DNA condensation order at which DNA duplication starts and the main weft of a three dimensional network within the interphase nucleus. (+info)
Pseudorabies virus expressing bovine herpesvirus 1 glycoprotein B exhibits altered neurotropism and increased neurovirulence. (5/114)Herpesvirus glycoproteins play dominant roles in the initiation of infection of target cells in culture and thus may also influence viral tropism in vivo. Whereas the relative contribution of several nonessential glycoproteins to neurovirulence and neurotropism of Pseudorabies virus (PrV), an alphaherpesvirus which causes Aujeszky's disease in pigs, has recently been uncovered in studies using viral deletion mutants, the importance of essential glycoproteins is more difficult to assess. We isolated an infectious PrV mutant, PrV-9112C2, which lacks the gene encoding the essential PrV glycoprotein B (gB) but stably carries in its genome and expresses the homologous gene of bovine herpesvirus 1 (BHV-1) (A. Kopp and T. C. Mettenleiter, J. Virol. 66:2754-2762, 1992). Apart from exhibiting a slight delay in penetration kinetics, PrV-9112C2 was similar in its growth characteristics in cell culture to wild-type PrV. To analyze the effect of the exchange of these homologous glycoproteins in PrV's natural host, swine, 4-week-old piglets were intranasally infected with 10(6) PFU of either wild-type PrV strain Kaplan (PrV-Ka), PrV-9112C2, or PrV-9112C2R, in which the PrV gB gene was reinserted instead of the BHV-1 gB gene. Animals infected with PrV-Ka and PrV-9112C2R showed a similar course of disease, i.e., high fever, marked respiratory symptoms but minimal neurological disorders, and excretion of high amounts of virus. All animals survived the infection. In contrast, animals infected with PrV-9112C2 showed no respiratory symptoms and developed only mild fever. However, on day 5 after infection, all piglets developed severe central nervous system (CNS) symptoms leading to death within 48 to 72 h. Detailed histological analyses showed that PrV-9112C2R infected all regions of the nasal mucosa and subsequently spread to the CNS preferentially by the trigeminal route. In contrast, PrV-9112C2 primarily infected the olfactory epithelium and spread via the olfactory route. In the CNS, more viral antigen and significantly more pronounced histological changes resulting in more severe encephalitis were found after PrV-9112C2 infection. Thus, our results demonstrate that replacement of PrV gB by the homologous BHV-1 glycoprotein resulted in a dramatic increase in neurovirulence combined with an alteration in the route of neuroinvasion, indicating that the essential gB is involved in determining neurotropism and neurovirulence of PrV. (+info)
DnaA, the initiator of Escherichia coli chromosomal replication, is located at the cell membrane. (6/114)Given the lack of a nucleus in prokaryotic cells, the significance of spatial organization in bacterial chromosome replication is only beginning to be fully appreciated. DnaA protein, the initiator of chromosomal replication in Escherichia coli, is purified as a soluble protein, and in vitro it efficiently initiates replication of minichromosomes in membrane-free DNA synthesis reactions. However, its conversion from a replicatively inactive to an active form in vitro occurs through its association with acidic phospholipids in a lipid bilayer. To determine whether the in situ residence of DnaA protein is cytoplasmic, membrane associated, or both, we examined the cellular location of DnaA using immunogold cryothin-section electron microscopy and immunofluorescence. Both of these methods revealed that DnaA is localized at the cell membrane, further suggesting that initiation of chromosomal replication in E. coli is a membrane-affiliated event. (+info)
Macrocryosectioning of the prostate: a simple technique. (7/114)Whole mount sections of the prostate are widely used in many laboratories. Macrocryosections of the gland; that is, whole mount frozen sections of the prostate from radical prostatectomies represent a useful new research protocol. The technique is very simple and does not require expensive equipment. (+info)
Apical accumulation of MARCKS in neural plate cells during neurulation in the chick embryo. (8/114)BACKGROUND: The neural tube is formed by morphogenetic movements largely dependent on cytoskeletal dynamics. Actin and many of its associated proteins have been proposed as important mediators of neurulation. For instance, mice deficient in MARCKS, an actin cross-linking membrane-associated protein that is regulated by PKC and other kinases, present severe developmental defects, including failure of cranial neural tube closure. RESULTS: To determine the distribution of MARCKS, and its possible relationships with actin during neurulation, chick embryos were transversely sectioned and double labeled with an anti-MARCKS polyclonal antibody and phalloidin. In the neural plate, MARCKS was found ubiquitously distributed at the periphery of the cells, being conspicuously accumulated in the apical cell region, in close proximity to the apical actin meshwork. This asymmetric distribution was particularly noticeable during the bending process. After the closure of the neural tube, the apically accumulated MARCKS disappeared, and this cell region became analogous to the other peripheral cell zones in its MARCKS content. Actin did not display analogous variations, remaining highly concentrated at the cell subapical territory. The transient apical accumulation of MARCKS was found throughout the neural tube axis. The analysis of another epithelial bending movement, during the formation of the lens vesicle, revealed an identical phenomenon. CONCLUSIONS: MARCKS is transiently accumulated at the apical region of neural plate and lens placode cells during processes of bending. This asymmetric subcellular distribution of MARCKS starts before the onset of neural plate bending. These results suggest possible upstream regulatory actions of MARCKS on some functions of the actin subapical meshwork. (+info)
Leica CM3050 S
Leica CM3050 S,The Leica CM3050 S Cryostat Family features superior user comfort with excellent safety standards for practically all types of cryosectioning applications. The Leica CM3050 S is the instrument of choice for all cryosectioning research applications and also for advanced clinical cryosectioning needs,medicine,medical supply,medical supplies,medical product
Visualization of brain monoamine oxidase B (MAO-B) in dementia of Alzheimers type by means of large cryosection...
Visualization of brain monoamine oxidase B (MAO-B) in dementia of Alzheimers type by means of large cryosection autoradiography: a pilot study. - S S Jossan, P G Gillberg, I Karlsson, C G Gottfries, L Oreland
The laboratory and workshop portions of the course provide hands-on introduction to engineering of mouse models, stem cell technologies and tissue analyses. Experimental techniques include genome editing by CRISPR/Cas9, pronuclear microinjection, isolation and culture/manipulation of pre- and post-implantation embryos, embryo transfer, embryo electroporation and roller bottle culture, chimera generation, generation and differentiation of mouse embryonic stem cells and fibroblasts, vibratome and cryosectioning, in situ RNA hybridization, immunostaining, FACS sorting and analysis of hematopoietic stem cells, skeletal preparation, organ explant culture and fluorescent imaging, including live time-lapse microscopy. ...
frozen frog oocytes
Im trying to section Xenopus oocytes frozen in OCT with poor results. Lots of cracking and shattering. Im figuring that temp plays a roll here and the little things are very yolky. Is there anybody out in histonetland who may have done some sectioning of frog oocytes or something similar? I actually found a Xenopus histo book but no suggestions for cryosectioning. As is usually the case here, the labs bring the tissue already frozen in OCT. What can I suggest they do prior to or during freezing the make the sections cut better? Does anybody know an optimal temp for cutting frog oocytes? Thanks for any help. Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: [email protected]) : ...
An Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution...
We have successfully developed a method for IAA analysis from small numbers of cells of specific cell types using cell sorting of GFP-expressing Arabidopsis lines followed by sensitive MS-based IAA measurements. Using this technique, cell-specific IAA quantification and biosynthesis measurements were possible from lines with GFP expression in as few as four to seven cells per root (pAGL42:GFP, QC cell expressing line) (Nawy et al., 2005). Previously, the only plant tissues in which auxins have been able to be measured at near-cellular resolution are the wood-forming tissues of various tree species, where gradients of IAA concentrations, with maxima in the cambial stem cells, have been observed after cryosectioning of the samples followed by MS analyses (Uggla et al., 1996).. Here, we selected 14 transgenic Arabidopsis lines that collectively express GFP in all cell types of the primary root apex. The lines were selected because they provided the most cell type-specific expression patterns ...
Protocols and Video Articles Authored by Edwin S. Monuki (Translated to Portuguese)
Edwin S. Monuki is the author of these articles in the Journal of Visualized Experiments: Fator-Revestido crescimento Placement Bead em explantes Encéfalo anterior Dorsal, Isolamento do mouse Remoção dorsais Encéfalo anterior, Cultura de rato precursores das células-tronco neurais, Flash Freezing e Cryosectioning E12.5 cérebro de camundongo, Extração de RNA a partir de células Neuroprecursor Usando o Bio-Rad Total de RNA Kit, A genotipagem rápida de tecido Mouse Usando Extract-N-Amp Tissue Sigma PCR Kit
Protocols and Video Articles Authored by D. Spencer Currle (Translated to Portuguese)
D. Spencer Currle is the author of these articles in the Journal of Visualized Experiments: Flash Freezing e Cryosectioning E12.5 cérebro de camundongo, Cultura de rato precursores das células-tronco neurais, Fator-Revestido crescimento Placement Bead em explantes Encéfalo anterior Dorsal, Rato de isolamento de células Adrenal Cromafim
Cryo Diamond Knives
A single slice of a tomogram of an aldehyde fixed and sucrose infiltrated cryosection with a 3D reconstruction. Erik Bos and Peter J. Peters, Netherlands Cancer Institute, Amsterdam. (see: J. Lefman, P. Zhang, T. Hirai, RM. Weis, J. Juliani, D. Bliss, M. Kessel, E. Bos, P.J. Peters, S. Subramaniam: Three-dimensional electron microscopic imaging of membrane invaginations in Echerichia coli overproducing the chemotaxis receptor Tsr. J. Bacteriol. 2004 Aug; 186(15): 5052-61 ...
SECOM: Correlative Light and Electron Microscopy | DELMIC
The SECOM platform is an integrated solution for correlative light and electron microscopythat enables you to do correlative microscopy extremely fast.
Correlative Microscopy at Leiden University Medical Centre - Linkam Scientific
A researcher at LUMC using the THMS600 to look at fluorescently labelled bacteria at liquid nitrogen temperature for correlative light electron microscopy. Professor A.J. Koster and his team at Leiden University Medical Centre are using the THMS600 in a correlative light and electron microscopy setup, to aid the cryo-study of biological specimens.. His group focuses on applications in cell biology, including the study of viral infections and viral replication where fluorescence may be used to pinpoint areas worthy of enhanced investigation. Also of particular interest is the field of vascular biology and the mechanism via which vascular endothelial cells initiate repair in response to injury and inflammation.. His goal is to localize molecular structures in cells using fluorescence microscopy and then transfer the sample to a cryo-electron microscopy (Cryo-EM) set up to image the corresponding macromolecular structures in 3D with nm-scale resolution.. The group wanted a cryo-FM sestup that was ...
Launch of the MirrorCLEM System for Correlative Light and Electron Microscopy : Hitachi High-Technologies in Canada
TOKYO, Japan, July 21, 2016 - Hitachi High-Technologies Corporation (TSE: 8036, Hitachi High-Tech) and RIKEN, one of Japans national institutes for scientific research, announced today that they have jointly developed MirrorCLEM, a system for simplifying correlative light and electron microscopy (CLEM) which enables the observation of one using both light and scanning electron microscopes (SEM). This new system will be launched for sale by Hitachi High-Tech on 25 July, 2016.. Various types of microscopes are used in a wide variety of fields such as nanotechnology, materials, medical and life sciences. In the medical and life sciences field in particular, SEMs are used to clarify the ultrastructure of cells and tissues, while a type of light microscope called fluorescence microscopes are being used increasingly to observe the localization and behavior of proteins at the molecular level. In recent years, CLEM techniques, which correlate electron microscopy with fluorescence microscopy, have ...
Andrea Falqui | Imaging & Microscopy - Research, Development, Production
Andrea Falqui. Jul. 30, 2017Science ITO Coating for Correlative Microscopy Correlative microscopy of immunolabeled cells performed by scanning electron and light microscopy needs a suitable coating.
New Cryo-correlative Microscopy Stage Greatly Improves Workflows Labmate Online
Linkam has been developing cryo stages for correlative microscopy for many years and continues to be at the forefront of cryo correlative microscopy with the latest update of their LINK software for the CMS196M, providing improved imaging capabilities and the new liquid nitrogen autofill system providing longer run times. Click to read more...
Correlative microscopy | Labsoft
Polski) Przypominamy o przetwarzaniu Twoich danych osobowych zgodnych z Rozporządzeniem Parlamentu Europejskiego i Rady (UE) 2016/679 z dnia 27 kwietnia 2016 r. oraz o zasadach, na jakich będzie się to odbywało. Zamykając ten komunikat, zgadzasz się w całości z regulaminem strony ...
High-pressure Freezing | Imaging & Microscopy - Research, Development, Production
High-pressure Freezing. Oct. 26, 2017Science Compression and Crevasses in Vitreous Sections under Different Cutting Conditions Wiley open access: A related research article, again with Jacques
Carl Zeiss to Exhibit Correlative Light and Electron Microscopy
Technology Networks is an internationally recognised publisher that provides access to the latest scientific news, products, research, videos and posters.
Ackerley, Cameron | Laboratory Medicine and Pathobiology --- University of Toronto
The Electron Microscopy facility in the Division of Pathology at the Hospital for Sick Children consists of an EM suite containing three transmission electron microscopes (TEM), a scanning electron microscope (SEM), a field emission SEM (FESEM) with a cryostage and a TEM / scanning transmission electron microscope TEM / STEM. There is a preparative laboratory for routine electron microscopy used primarily for preparing clinical diagnostic specimens as well as preparing specimens for external researchers who wish to do electron microscopy.. In my adjoining laboratory we have all of the preparative equipment required for experimental procedures including, cryofixation for freeze substitution and cryoultramicrotomy and the preparation of thin films. In my laboratory, we have developed novel techniques such as high resolution FESEM secondary electron imaging of tissues and macromolecules in a frozen hydrated state and the ability to image cells, viral particles and large macromolecules in a ...
In situ biomarker discovery and label-free molecular histopathological diagnosis of lung cancer by ambient mass spectrometry...
Sensitive and spatial exploration of the metabolism of tumors at the metabolome level is highly challenging. In this study, we developed an in situ metabolomics method based on ambient mass spectrometry imaging using air flow-assisted desorption electrospray ionization (AFADESI), which can spatially explore the alteration of global metabolites in tissues with high sensitivity. Using this method, we discovered potential histopathological diagnosis biomarkers (including lipids, amino acids, choline, peptides and carnitine) from 52 postoperative lung cancer tissue samples and then subsequently used these biomarkers to generate images for rapid and label-free histopathological diagnosis. These biomarkers were validated with a sensitivity and a specificity of 93.5% and 100%, respectively. Moreover, a single imaging analysis of a cryosection that visualized all these biomarkers, taking tens of minutes, revealed the type and subtype of the cancer. This method could potentially be used as a molecular
For most doctors, any mention of preclinical anatomy teaching evokes memories of the smell of formalin. Medical students in the future, however, are just as likely to use a computer as a scalpel to explore human anatomy, thanks to the Visible Human Project: http://www.nlm.nih.gov/research/visible/visible_human.html. This project, initiated by the US National Library of Medicine, aims to create complete, anatomically detailed, three dimensional representations of male and female human bodies. Transverse computed tomographic, magnetic resonance imaging, and cryosection images have already been collected at 1 mm intervals from a male and a female cadaver-sample images and animations are available on the projects web site ...
Visible Human Project: Anatomy of the whole human body (male cadaver)
The Visible Human Project® is an outgrowth of the NLMs 1986 Long-Range Plan. It is the creation of complete, anatomically detailed, three-dimensional representations of the normal male human body. Cryosection images of representative male cadaver has been completed. The male was sectioned at one millimeter intervals,
Clinical Pathology - Acibadem City Clinic Mladost
Samples (biopsies) taken during surgery to be examined immediately by a cryosection for rapid diagnosis and it can help when resolving about the volume of surgery intervention - in breast surgeries, the genitourinary system, the GI tract, etc. These same samples are subject to a permanent paraffin treatment in regards to providing a precise diagnosis within 2-3 days ...
Correlative light and electron microscopy of autophagosomes<...
TY - CHAP. T1 - Correlative light and electron microscopy of autophagosomes. AU - Gudmundsson, Sigurdur Runar. AU - Kahlhofer, Jenny. AU - Baylac, Nastassia. AU - Kallio, Katri Anneli. AU - Eskelinen, Eeva-Liisa. PY - 2019/1. Y1 - 2019/1. N2 - Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.. AB - Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.. KW - 1182 Biochemistry, cell and molecular ...
Correlative light electron microscopy of giant viruses with the SECOM system - Aix-Marseille Université
J.-P. Baudoin, S. Hari, D. Sahmi-Bounsiar, S. Traoré, J. Bou-Khalil, et al.. Correlative light electron microscopy of giant viruses with the SECOM system. New Microbes and New Infections, Wiley Online Library 2018, 26, pp.110-113. ⟨10.1016/j.nmni.2018.09.006⟩. ⟨hal-02006237⟩ ...
Endophthalmitis | OphEd
Reading Assignment: There are several sections in the Retina and Vitreous section of the BCSC concerning endophthalmitis. Please read through these. In addition, please review the EVS-link attached. ...
Fujiwara, Yoshiyuki - Authors - Tottori University research result repository
Tokuyasu, Naruo/ Shomori, Kohei/ Amano, Kuniki/ Honjo, Soichiro/ Sakamoto, Teruhisa/ Watanabe, Joji/ Amisaki, Masataka/ Morimoto, Masaki/ Uchinaka, Ei/ Yagyu, Takuki/ Saito, Hiroaki/ Ito, Hisao/ Fujiwara, Yoshiyuki/ ...
RE: [Histonet] IHC on brain cryosections... help!!
I agree with Geoff McAuliffe in every detail, you need to perfuse, and you need to fast freeze. If perfusion is new to you, I suggest you review the manual and protocol posted on the left at the following link. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=471001&catdesc=Histology+Equipment&CatThreeID=674&CatOneID=4&subcatdesc=Sacrifice+Equipment&idsubcategory=21 About the need for fast freezing, see http://www.myneurolab.com/global/Manuals/Tips%20and%20Techniques%20Freezing%20Artifact.pdf Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 FAX 314 522 0377 [email protected] http://www.myneurolab.com -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Anna Elisse Beaudin Sent: Tuesday, December 07, 2004 2:29 PM To: [email protected] Subject: [Histonet] IHC on brain cryosections... help!! Hi all, I am ...
New ZEN technology for Microscopy | SelectScience
Learn about the newly expanded ZEN technology and new Correlative Microscopy in this interview with John B Yorston, Carl Zeiss. Capabilities of the Sigma VP Field Emission Scanning Electron Microscope are demonstrated and the LSM 700 Scanning Confocal Microscope is shown. These systems combine ultra-resolution with labelling capabilities for a turn-key solution called Shuttle and Find. Filmed by SelectScience at ASCB 2011.
SEM Analysis of Surface Impact on Biofilm Antibiotic Treatment
Scanning provides an international and interdisciplinary medium for the rapid exchange of information among all scientists interested in scanning electron, scanning probe, and scanning optical microscopies. Areas of specific interest include all aspects of the instrumentation associated with scanning microscopies, correlative microscopy techniques, stereometry, stereology, analytic techniques, and novel applications of the microscopies.
Cryostat lower cylinder: readied for welding
It is one thing to know that the cryostat will be 30 metres high and 30 metres in diameter; it is another to see these dimensions translated into reality. [...]
Real-Time In Situ Correlative Microscopy for Cellular Biology - Australian Microscopy and Microanalysis Society
Sangeetha obtained her Bachelor of Science (Honours) in 2005 and Masters degree (2007) from The University of Delhi, India. She went on to work as a Research Scholar in atomic and molecular physics at the Tata Institute of Fundamental Research, Mumbai, India, until 2011, developing time-of-flight spectroscopy systems for the study of electron-molecule collisions and specific bond breaking in molecules. She went on to do a Ph.D. at the TU Delft (until 2016), The Netherlands, working on a novel technology for sub-30 nm lithography in the Scanning Electron Microscope. She developed imaging techniques to extract quantitative information from high resolution SEM images, using a combination of secondary and backscattered electron imaging with atomic force microscopy. She now works at Delmic BV as an applications specialist in correlative light and electron microscopy, developing new applications with the SECOM platform.. ...
Hyalocytes in idiopathic epiretinal membranes: a correlative light and electron microscopic study. - literature database -...
To describe characteristics of epiretinal cells at the vitreoretinal interface by correlative light and electron microscopy (CLEM).Epiretinal membrane (ERM) specimens and internal limiting membrane (I...
Array Tomography is one way to acquire 3D data series of ultra or semi-thin sections of a tissue sample. Thereby, the sample is cut with an ultramicrotome and the sections are collected on a substrate for subsequent imaging with a SEM. Depending on the research project more or less sections are required to completely reconstruct a region of interest.. In comparison to Serial Block-Face Scanning EM (SBFSEM) and Focused Ion Beam (FIB), Array Tomography is a non-destructive technique. The collected sections can be stored, re-imaged at difference regions and resolutions, re-stained with heavy metals to enhance contrast or with fluorescent markers for correlative light and electron microscopy (CLEM). By now, Array Tomography is not only used for Connectomics but also in other fields of research, e.g. single cell reconstructions or even to gain 3D data of complex materials.. References Q&A: Array tomography. Smith SJ; BMC Biol. 2018 Sep 6;16(1):98.. A targeted 3D EM and correlative microscopy method ...
CIL:40609, Saccharomyces cerevisiae. CIL. Dataset
A 5 nm tomographic slice from a vitreous section of a Saccharomyces cerevisiae cell. (M) is a Mitochondrion and (V) a vacuole. Scale bar, 100 nm. Uppe...
Photoactivated Localization Microscopy (PALM) is a super-resolution imaging technique that achieves nanometre-scale resolution by exploiting the properties of photoactivatable fluorophores to reveal spatial details of tightly packed molecules.
RMS | Virtual Frontiers in Bioimaging 2020
Leandro obtained his PhD in Biophysics in Brazil, studying the cellular structure of parasites and the interaction with host cells. He did a Post-doc at the University of Heidelberg (Germany) working on the cytoskeleton dynamics of the Malaria parasite Plasmodium sp and its structural organization. He took a position as a Research Support Specialist at the Electron Microscopy Resource Center of The Rockefeller University (New York, USA) and afterwards a position of Research Associate at the National Technology Institute in Brazil. Since 2015 he manages the Imaging sector of the Wellcome Centre for Molecular Parasitology at the University of Glasgow. Since his PhD, Leandro has applied optical, electron, super-resolution and cryo-microscopy in his work, as well as correlative microscopy.. ...
Microscope Products for Ultrastructural Investigations
Learn how your ultrastructural investigations can benefit from modern electron microscope techniques. Correlative microscopy provides you deeper insights about cell structure by combination of light and scanning electron microscopy methods.
Focus on Microscopy (FOM) 2014
Visit our booth at FOM 2014 and discover ZEISS systems for light sheet fluorescence microscopy (LSFM), superresolution and correlative microscopy.
Distribution of TRP1 in the Golgi and associated vesicl | Open-i
Distribution of TRP1 in the Golgi and associated vesicles. (A) Ultrathin cryosections of MNT-1 cells were labeled with anti-TRP1 and PAG 5. TRP1 is often enrich
MR images of the intratemporal portion of the facial nerve were obtained with surface coils using a 0.3-T permanent magnet whole-body imaging system. Various 20FT spin-echo pulse sequences were used to produce 5-mm thick sections with 0.5-mm pixels on a 512 × 512 acquisition matrix. The MR images from normal volunteers were correlated with cryosection specimens of three fresh human cadavers. The seventh nerve was followed in the internal auditory and fallopian canal and through temporal bone to the stylomastoid foramen. The entire labyrinthine, tympanic, and mastoid portions, as well as the geniculate ganglion, could be shown with appropriate scan planes. MR produces excellent images of the facial nerve with high-contrast resolution. Unlike CT, no beam-hardening artifact from the temporal bone is apparent. MR should be a sensitive study for the evaluation of intratemporal facial nerve disease.. ...
Cores | OHSU Research News
The Advanced Light Microscopy Core will be holding a Focus on Microscopy forum on Friday, August 2, 2013. The event will be held at the Jungers Center in Vollum M1441 from 2 to 4:30 p.m. The forum will feature presentations on: New EM Capabilities at OHSU by Chris Arthur, Ph.D., from the FEI Living Lab Devil in the Details: Sample Processing for Electron Superressolution and Correlative Light Microscopy by Danielle Robinson from the Zhong Lab Photoactivated Localization Microscopy with … Read More. ...
Responsible Nanotechnology: Microscopy Revolutions
Two new and very cool microscopy techniques have been announced recently. One, the optofluidic microscope, could put an entire microscope, including display, into a device the size of an iPod. The other, photoactivated localization microscopy (PALM), was invented by two...
Elyra P.1 3-D Superresolution Microscopy | Carl Zeiss Spectroscopy GmbH | Feb 2014 | BioPhotonics
Zeiss has added the Elyra P.1 module to its 3-D microscopy portfolio. The system enables superresolution photoactivated localization microscopy (PALM)
Cryosections of normal and diabetic rat retinas immunos | Open-i
Cryosections of normal and diabetic rat retinas immunostained with anti-LOX primary antibody and rhodamine-conjugated secondary antibody. Blood vessel in the di
ZEISS Introduces New Technology for 3D Superresolution Microscopy | SelectScience
Expanding its 3D microcopy portfolio, ZEISS introduced superresolution photoactivated localization microscopy (PALM) in 3D at the Society for Neuroscience Annual Meeting in San Diego, California.
Sydney Research Online: Browsing Sydney Research Online
Assessment of different fixation protocols on the presence of membrane-bound vesicles in Caco-2 cells: A multidimensional view by means of correlative light and 3-D transmission electron microscopy, Micron, vol.67, N/A, 2014,pp 20-29 ...
Cryo-Electron Microscopy Is the Method of the Year 2015 | Labcritics
Nature Methods has named Cryo-Electron Microscopy the method of the year 2015. This structural technique, which was always a step behind
Correlative anatomy of the nervous system - کتابخانه الکترونیک و دیجیتال - آذرسا
Correlative anatomy of the nervous system , Correlative anatomy of the nervous system , کتابخانه الکترونیک و دیجیتال - آذرسا
MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy | eLife
Individuals with congenital amusia have a lifelong history of unreliable pitch processing. Accordingly, they downweight pitch cues during speech perception and instead rely on other dimensions such as duration. We investigated the neural basis for this strategy. During fMRI, individuals with amusia (N=15) and controls (N=15) read sentences where a comma indicated a grammatical phrase boundary. They then heard two sentences spoken that differed only in pitch and/or duration cues, and selected the best match for the written sentence. Prominent reductions in functional connectivity were detected in the amusia group, between left prefrontal language-related regions and right hemisphere pitch-related regions, which reflected the between-group differences in cue weights in the same groups of listeners. Connectivity differences between these regions were not present during a control task. Our results indicate that the reliability of perceptual dimensions is linked with functional connectivity between ...
The combination of neutrophil-to-lymphocyte ratio and serum carbohydrate antigen 19-9 level as a prognostic indicator in...
TY - JOUR. T1 - The combination of neutrophil-to-lymphocyte ratio and serum carbohydrate antigen 19-9 level as a prognostic indicator in patients with recurrent pancreatic cancer. AU - Sakamoto, Teruhisa. AU - Saito, Hiroaki. AU - Uchinaka, Ei. AU - Morimoto, Masaki. AU - Amisaki, Masataka. AU - Tokuyasu, Naruo. AU - Honjo, Soichiro. AU - Ashida, Keigo. AU - Fujiwara, Yoshiyuki. PY - 2018/9. Y1 - 2018/9. N2 - Background/Aim: We retrospectively investigated the relationship between prognosis and combined neutrophil-to-lymphocyte ratio (NLR) and serum carbohydrate antigen 19-9 (CA19-9) levels in patients with recurrent pancreatic cancer. Patients and Methods: We enrolled 66 patients whose pancreatic cancer recurred. Results: Based on ROC analysis results, the patients were divided into NLRHigh (NLR ≥1.69) or NLRLow (NLR ,1.69), and into CA19-9High (CA19-9 ≥107.95 U/ml) or CA19-9Low (CA19-9 ,107.95 U/ml). When the patients were grouped by combined NLR and CA19-9, their 2-year survival rates ...
A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of...
The assembly process of the human immunodeficiency virus 1 (HIV-1) is driven by the viral polyprotein Gag. Fluorescence imaging of Gag protein fusions is widely performed and has revealed important information on viral assembly. Gag fusion proteins are commonly co-transfected with an unlabeled form of Gag to prevent labeling artifacts such as morphological defects and decreased infectivity. Although viral assembly is widely studied on individual cells, the efficiency of the co-transfection rescue has never been tested at the single cell level. Here, we first develop a methodology to quantify levels of unlabeled to labeled Gag in single cells using a fluorescent reporter protein for unlabeled Gag and fluorescence correlation spectroscopy. Using super-resolution imaging based on photoactivated localization microscopy (PALM) combined with molecular counting we then study the nanoscale morphology of Gag clusters as a function of unlabeled to labeled Gag ratios in single cells. We show that for a ...
Diamond Publications - Publication
Abstract: The vertebrate sodium (Nav) channel is composed of an ion-conducting alpha subunit and associated beta subunits. Here, we report the crystal structure of the human beta 3 subunit immunoglobulin (Ig) domain, a functionally important component of Nav channels in neurons and cardiomyocytes. Surprisingly, we found that the beta 3 subunit Ig domain assembles as a trimer in the crystal asymmetric unit. Analytical ultracentrifugation confirmed the presence of Ig domain monomers, dimers, and trimers in free solution, and atomic force microscopy imaging also detected full-length beta 3 subunit monomers, dimers, and trimers. Mutation of a cysteine residue critical for maintaining the trimer interface destabilized both dimers and trimers. Using fluorescence photoactivated localization microscopy, we detected full-length beta 3 subunit trimers on the plasma membrane of transfected HEK293 cells. We further show that beta3 subunits can bind to more than one site on the Nav 1.5 alpha subunit and ...
Can the NucView® caspase-3 substrates be used on (PFA or methanol) fixed cells or tissue cryosections? | Biotium
The NucView® caspase-3 substrates are activity-dependent i.e. require active caspase-3 enzyme. In dead, fixed and preserved cells or tissue sections, there are no active caspases and hence these substrates cannot be used. We also offer TUNEL assay kits that are suitable for apoptosis detection in fixed cells and tissues. The kits employ dUTPs conjugated to our exceptionally bright and photostable CF® dyes for single-step fluorescent TUNEL labeling of DNA strand breaks, a hallmark of apoptotic cells, and are suitable for analysis by fluorescence microscopy or flow cytometry.. ...
Advancing Cryo-Electron Microscopy for Life Sciences
Kristina Kermanshahche demos Cryo-Electron Microscopy for mapping protein structures on the Intel® Scalable System Framework at Supercomputing 2016.
Figure 5 | Scientific Reports
Modulation of p43 signaling affects early muscle fibrosis. (A,C) Sirius Red staining on cryosections of regenerated Tibialis muscles in wild-type, p43−/− and p43-Tg mice at 28 d.p.i and 70.d.p.i. (B,D) Quantification of Sirius Red staining (H) muscles in wild-type, p43−/− and p43-Tg mice at 28 d.p.i. and 70.d.p.i. (n = 6 for each group). Statistical significance: *p , 0.05; **p , 0.01; ***p , 0.001. Students t-test. Results are expressed as ± sem. ...
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He contributed to the development of the electron microscope and was the first person to use the concept of cryo-ultramicrotomy ...
Central University of Venezuela
... to the development of the electron microscope and was the first researcher to introduce the concept of cryoultramicrotomy. ...
In 1952 Humberto Fernandez Morán introduced cryo ultramicrotomy, which is a similar technique but done at freezing temperatures ... between −20 and −150°C. Cryo ultramicrotomy can be used to cut ultra-thin frozen biological specimens. One of the advantages ...
List of MeSH codes (E05)
... cryoultramicrotomy MeSH E05.200.500.620.530.160.260 - frozen sections MeSH E05.200.500.620.620 - replica techniques MeSH ... cryoultramicrotomy MeSH E05.200.750.600.530.160.260 - frozen sections MeSH E05.200.750.600.620 - replica techniques MeSH ...
UCD Conway Institute of Biomolecular and Biomedical Research | Electron Microscopy
Optimization of FIB-SEM Tomography and Reconstruction for Soft, Porous, and Poorly Conducting Materials | Microscopy and...
Microtomy | Profiles RNS
Hamzei-Sichani F, Kamasawa N, Janssen WG, Yasumura T, Davidson KG, Hof PR, Wearne SL, Stewart MG, Young SR, Whittington MA, Rash JE, Traub RD. Gap junctions on hippocampal mossy fiber axons demonstrated by thin-section electron microscopy and freeze fracture replica immunogold labeling. Proc Natl Acad Sci U S A. 2007 Jul 24; 104(30):12548-53 ...
Electron Microscopy Sciences | EMS
Analysis of Multibeam Milling of Soft Films
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MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
Professor Silvia Vignolini | Page 4 | Yusuf Hamied Department of Chemistry
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MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
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Windows-based RC-010 remote control software allows Safematic CCU-010 coaters to be run remotely for the control, monitoring and analysis of coating processes, for example in a glove box. The RC-010 comes ready with pre-defined recipes with optimal settings for the most common metal sputtering targets as well as for carbon. Requires connection from a laptop or computer (not supplied) to the CCU-010 coating system via a USB cable.. ...