Cryoprotective Agents: Substances that provide protection against the harmful effects of freezing temperatures.Ethylene Glycol: A colorless, odorless, viscous dihydroxy alcohol. It has a sweet taste, but is poisonous if ingested. Ethylene glycol is the most important glycol commercially available and is manufactured on a large scale in the United States. It is used as an antifreeze and coolant, in hydraulic fluids, and in the manufacture of low-freezing dynamites and resins.Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Freezing: Liquids transforming into solids by the removal of heat.Propylene Glycol: A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations.Dimethyl Sulfoxide: A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.Semen Preservation: The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Glycerol: A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.Sperm Motility: Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.Cell Membrane Permeability: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.Spermatozoa: Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.Ice: The solid substance formed by the FREEZING of water.Temperature: The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.Preservation, Biological: The process of protecting various samples of biological material.Antarctic Regions: The continent lying around the South Pole and the southern waters of the Atlantic, Pacific, and Indian Oceans. It includes the Falkland Islands Dependencies. (From Webster's New Geographical Dictionary, 1988, p55)Chironomidae: A family of nonbiting midges, in the order DIPTERA. Salivary glands of the genus Chironomus are used in studies of cellular genetics and biochemistry.DimethylformamidePovidone: A polyvinyl polymer of variable molecular weight; used as suspending and dispersing agent and vehicle for pharmaceuticals; also used as blood volume expander.Dehydration: The condition that results from excessive loss of water from a living organism.TrehaloseL-Lactate Dehydrogenase: A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.Acclimatization: Adaptation to a new environment or to a change in the old.Sucrose: A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.Cold Temperature: An absence of warmth or heat or a temperature notably below an accustomed norm.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Research Report: Detailed account or statement or formal record of data resulting from empirical inquiry.Mortuary Practice: Activities associated with the disposition of the dead. It excludes cultural practices such as funeral rites.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Software: Sequential operating programs and data which instruct the functioning of a digital computer.ArchivesApolipoprotein B-48: A 241-kDa protein synthesized only in the INTESTINES. It serves as a structural protein of CHYLOMICRONS. Its exclusive association with chylomicron particles provides an indicator of intestinally derived lipoproteins in circulation. Apo B-48 is a shortened form of apo B-100 and lacks the LDL-receptor region.Apolipoprotein B-100: A 513-kDa protein synthesized in the LIVER. It serves as the major structural protein of low-density lipoproteins (LIPOPROTEINS, LDL; LIPOPROTEINS, VLDL). It is the ligand for the LDL receptor (RECEPTORS, LDL) that promotes cellular binding and internalization of LDL particles.Fatty Acids, Nonesterified: FATTY ACIDS found in the plasma that are complexed with SERUM ALBUMIN for transport. These fatty acids are not in glycerol ester form.Lipoproteins: Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes.Anomura: An infraorder of CRUSTACEA, in the order DECAPODA comprising the hermit crabs and characterized by a small fifth pair of legs.TriglyceridesInfusions, Intravenous: The long-term (minutes to hours) administration of a fluid into the vein through venipuncture, either by letting the fluid flow by gravity or by pumping it.Honey: A sweet viscous liquid food, produced in the honey sacs of various bees from nectar collected from flowers. The nectar is ripened into honey by inversion of its sucrose sugar into fructose and glucose. It is somewhat acidic and has mild antiseptic properties, being sometimes used in the treatment of burns and lacerations.Glucose Oxidase: An enzyme of the oxidoreductase class that catalyzes the conversion of beta-D-glucose and oxygen to D-glucono-1,5-lactone and peroxide. It is a flavoprotein, highly specific for beta-D-glucose. The enzyme is produced by Penicillium notatum and other fungi and has antibacterial activity in the presence of glucose and oxygen. It is used to estimate glucose concentration in blood or urine samples through the formation of colored dyes by the hydrogen peroxide produced in the reaction. (From Enzyme Nomenclature, 1992) EC 1.1.3.4.Apitherapy: The medical use of honey bee products such as BEE VENOM; HONEY; bee pollen; PROPOLIS; and royal jelly.Leptospermum: A plant genus of the family MYRTACEAE. The common name of tea tree is also used for MELALEUCA and KUNZEA.Magnesium Oxide: Magnesium oxide (MgO). An inorganic compound that occurs in nature as the mineral periclase. In aqueous media combines quickly with water to form magnesium hydroxide. It is used as an antacid and mild laxative and has many nonmedicinal uses.Fluorometry: An analytical method for detecting and measuring FLUORESCENCE in compounds or targets such as cells, proteins, or nucleotides, or targets previously labeled with FLUORESCENCE AGENTS.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Thumb: The first digit on the radial side of the hand which in humans lies opposite the other four.Papua New Guinea: A country consisting of the eastern half of the island of New Guinea and adjacent islands, including New Britain, New Ireland, the Admiralty Islands, and New Hanover in the Bismarck Archipelago; Bougainville and Buka in the northern Solomon Islands; the D'Entrecasteaux and Trobriand Islands; Woodlark (Murua) Island; and the Louisiade Archipelago. It became independent on September 16, 1975. Formerly, the southern part was the Australian Territory of Papua, and the northern part was the UN Trust Territory of New Guinea, administered by Australia. They were administratively merged in 1949 and named Papua and New Guinea, and renamed Papua New Guinea in 1971.Propylene Glycols: Derivatives of propylene glycol (1,2-propanediol). They are used as humectants and solvents in pharmaceutical preparations.Image Processing, Computer-Assisted: A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.Carpometacarpal Joints: The articulations between the CARPAL BONES and the METACARPAL BONES.Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase: An amidohydrolase that removes intact asparagine-linked oligosaccharide chains from glycoproteins. It requires the presence of more than two amino-acid residues in the substrate for activity. This enzyme was previously listed as EC 3.2.2.18.

Freeze-fracture replication of organized tissue without cryoprotection. (1/576)

Fresh pieces of rat liver and pancreas were rapidly frozen without prior chemical fixation or cryoprotection, and replicated folloing freeze-fracture. Replicas revealed small peripheral areas free of ice crystals or damage and, within such areas, general ultrastructural morphology was essentially similar to that seen in conventionally processed material. On fracture faces of plasma and nuclear membranes a population of less prominent particles in addition to conventional membrane-associated particles was seen, and smooth areas devoid of particles of any type were seen on some nuclear membranes. These smooth areas did not appear to be similar to smooth areas allegedly arising as artifacts of conventional processing. Tight junctions and gap junctions appeared as they do in cryoprotected specimens. The results provide a base-line for assessing the possible effects of processing steps or agents on the ultrastructure of organized tissues as revealed in freeze-fracture replicas.  (+info)

Vitrification of mouse germinal vesicle oocytes: effect of treatment temperature and egg yolk on chromatin and spindle normality and cumulus integrity. (2/576)

The success rates for cryopreservation of immature oocytes from several species including human remain low, in contrast to major improvements with mature oocytes. In this study, a new approach has been developed using a short exposure ultra-rapid freezing protocol, examining the effect of temperature and egg yolk (two factors which may be expected to influence membrane flexibility) on the cryostability of immature mouse oocytes and cumulus complexes. These two factors were tested in various patterns for their cryoprotective effect using ethylene glycol as the principal cryoprotectant. The results showed that 37 degrees C pre- and post-freeze exposure significantly improved both survival and normal spindle configuration after in-vitro maturation. Egg yolk was found to produce further beneficial effects on both the oocyte and cumulus cell integrity, with the best effects being obtained at 37 degrees C with inclusion of egg yolk both before and after the freezing. This protocol produced > 80% normal survival post-thaw with intact and attached cumulus complex, 84% maturation rate and 45% normal metaphase configuration. In summary, a unique combination of high survival and meiotic normality together with good preservation of the attached cumulus cell mass has been achieved using a simple new vitrification procedure.  (+info)

Effects of commonly used cryoprotectants on glycogen phosphorylase activity and structure. (3/576)

The effects of a number of cryoprotectants on the kinetic and structural properties of glycogen phosphorylase b have been investigated. Kinetic studies showed that glycerol, one of the most commonly used cryoprotectants in X-ray crystallographic studies, is a competitive inhibitor with respect to substrate glucose-1-P with an apparent Ki value of 3.8% (v/v). Cryogenic experiments, with the enzyme, have shown that glycerol binds at the catalytic site and competes with glucose analogues that bind at the catalytic site, thus preventing the formation of complexes. This necessitated a change in the conditions for cryoprotection in crystallographic binding experiments with glycogen phosphorylase. It was found that 2-methyl-2,4-pentanediol (MPD), polyethylene glycols (PEGs) of various molecular weights, and dimethyl sulfoxide (DMSO) activated glycogen phosphorylase b to different extents, by stabilizing its most active conformation, while sucrose acted as a noncompetitive inhibitor and ethylene glycol as an uncompetitive inhibitor with respect to glucose-1-P. A parallel experimental investigation by X-ray crystallography showed that, at 100 K, both MPD and DMSO do not bind at the catalytic site, do not induce any significant conformational change on the enzyme molecule, and hence, are more suitable cryoprotectants than glycerol for binding studies with glycogen phosphorylase.  (+info)

The cryopreservation protocol optimal for progenitor recovery is not optimal for preservation of marrow repopulating ability. (4/576)

The efficiency of five different cryopreservation protocols (our original controlled-rate and noncontrolled-rate protocols) was evaluated on the basis of the recovery after thawing of very primitive pluripotent hemopoietic stem cells (MRA(CFU-GM), pluripotent progenitors (CFU-Sd12) and committed granulocyte-monocyte progenitors (CFU-GM) in mouse bone marrow. Although the nucleated cell recovery and viability determined immediately after the thawing and washing of the cells were found to be similar, whether controlled-rate or noncontrolled-rate cryopreservation protocols were used, the recovery of MRA(CFU-GM), CFU-Sd12 and CFU-GM varied depending on the type of protocol and the cryoprotector (DMSO) concentrations used. It was shown that the controlled-rate protocol was more efficient, enabling better MRA(CFU-GM), CFU-Sd12 and CFU-GM recovery from frozen samples. The most efficient was the controlled-rate protocol of cryopreservation designed to compensate for the release of fusion heat, which enabled a better survival of CFU-Sd12 and CFU-GM when combined with a lower (5%) DMSO concentration. On the contrary, a satisfactory survival rate of very primitive stem cells (MRA(CFU-GM)) was achieved only when 10% DMSO was included with a five-step protocol of cryopreservation. These results point to adequately used controlled-rate freezing as essential for a highly efficient cryopreservation of some of the categories of hematopoietic stem and progenitor cells. At the same time, it was obvious that a higher DMSO concentration was necessary for the cryopreservation of very primitive stem cells, but not, however, for more mature progenitor cells (CFU-S, CFU-GM). These results imply the existence of a mechanism that decreases the intracellular concentration of DMSO in primitive MRA cells, which is not the case for less primitive progenitors.  (+info)

Differences in the width of the intercellular spaces in the epithelial basal infolding and the renal glomerular filtration site between freeze-substitution and conventional fixation. (5/576)

After aldehyde prefixation, pretreatment with cryoprotectant and subsequent freeze-substitution with OsO4 in acetone (AC-FS), extensive gap junction-like close membrane appositions are frequently found in the basal infolding of the salivary gland epithelium, although the desmosomal intercellular space had the same width as with conventional electron microscopy. The intercellular space between podocyte pedicles and endothelial cells at the renal glomerular filtration site was narrower by the total width of 2 laminae lucidae following AC-FS than with conventional electron microscopy and was occupied by a homogeneous lamina densa without a lamina lucida, although no marked difference was discernable in the thickness of the lamina densa itself between the 2 preparative procedures. In addition, a decrease in the thickness of the glycocalyx was evident in the intestinal epithelial microvilli following AC-FS. It is thus likely that osmication in acetone at freezing temperatures remove the glycocalyx and related structures to a variable extent, and that this loss is responsible for reducing the intercellular spaces at some of the simple appositions narrower to the dimensions of the gap junction. It is also responsible for disappearance of the lamina lucida of the basement membrane.  (+info)

Ooplasmic injections of rabbit round spermatid nuclei or intact round spermatids from fresh, cryopreserved and cryostored samples. (6/576)

We compared the outcome of ooplasmic round spermatid nuclear injections (ROSNI) versus intact round spermatid injections (ROSI). Rabbit round spermatid nuclei and intact round spermatids were recovered and injected into rabbit oocytes (groups A and B, respectively). Fertilization, cleavage and embryonic development rates were compared. In additional studies, five protocols for cryopreservation of round spermatids and two protocols for cryostorage of round spermatids were applied. The outcome of ROSNI techniques using frozen-thawed or cryostored-warmed round spermatids was evaluated. The cleavage rate and the overall morula plus blastocyst development rate were significantly larger in group A than group B. ROSNI procedures are superior to ROSI techniques in the rabbit. The largest fertilization, cleavage and embryonic development rates after ROSNI techniques using cryopreserved or cryostored round spermatids were demonstrated in groups of round spermatids in which a mixture of seminal plasma plus test yolk buffer was employed as an extender, and dimethyl sulphoxide plus a high concentration of glycerol served as cryoprotectants. It appears that the seminal plasma contains factors protecting round spermatids during cryopreservation or cryostorage, and/or the employment of two cryoprotectants has a beneficial role in the maintenance of round spermatid reproductive capacity.  (+info)

Effects of cryoprotectants and ice-seeding temperature on intracellular freezing and survival of human oocytes. (7/576)

The accurate determination of the freezing conditions that promote intracellular ice formation (IIF) is crucial for designing cryopreservation protocols for cells. In this paper, the range of temperatures at which IIF occurs in human oocytes was determined. Fresh oocytes with a germinal vesicle, failed-to-fertilize (metaphase I and metaphase II stages) and polyspermic eggs were used for this study. The occurrence of IIF was first visualized at a cooling rate of 120 degrees C/min using a programmable thermal microscope stage connected to a videomicroscope. Then, with a cooling rate of 0.2 degrees C/min, the seeding temperature of the extracellular ice was modified to decrease the incidence of IIF and increase the survival rate of frozen-thawed human oocytes. After adding different cryoprotectants, the median temperature of IIF (TMED) was decreased by approximately 23 degrees C in mouse and only by approximately 6.5 degrees C in human oocytes. Using 1.5 M propylene glycol and seeding temperatures of -8.0, -6.0 and -4.5 degrees C, the incidence of IIF was 22/28 (78%), 8/24 (33%) and 0/33 (0%) and the 24 h post-thaw survival rate was 10/31(32%), 19/34 (56%) and 52/56 (93%) respectively. The results show that IIF occurs more readily in human oocytes, and that ice seeding between -6 degrees C and -8 degrees C triggers IIF in a large number of human oocytes. Undesirable IIF can be prevented and survival rates maximized by raising the seeding temperature as close as possible to the melting point of the solution, which in our instrument was -4.5 degrees C.  (+info)

Subzero water permeability parameters of mouse spermatozoa in the presence of extracellular ice and cryoprotective agents. (8/576)

Optimization of techniques for cryopreservation of mammalian sperm is limited by a lack of knowledge regarding water permeability characteristics during freezing in the presence of extracellular ice and cryoprotective agents (CPAs). Cryomicroscopy cannot be used to measure dehydration during freezing in mammalian sperm because they are highly nonspherical and their small dimensions are at the limits of light microscopic resolution. Using a new shape-independent differential scanning calorimeter (DSC) technique, volumetric shrinkage during freezing of ICR mouse epididymal sperm cell suspensions was obtained at cooling rates of 5 and 20 degrees C/min in the presence of extracellular ice and CPAs. Using previously published data, the mouse sperm cell was modeled as a cylinder (122-microm long, radius 0.46 microm) with an osmotically inactive cell volume (V(b)) of 0.61V(o), where V(o) is the isotonic cell volume. By fitting a model of water transport to the experimentally obtained volumetric shrinkage data, the best-fit membrane permeability parameters (L(pg) and E(Lp)) were determined. The "combined best-fit" membrane permeability parameters at 5 and 20 degrees C/min for mouse sperm cells in solution are as follows: in D-PBS: L(pg) = 1.7 x 10(-15) m(3)/Ns (0.01 microm/min-atm) and E(Lp) = 94.1 kJ/mole (22.5 kcal/mole) (R(2) = 0.94); in "low" CPA media (consisting of 1% glycerol, 6% raffinose, and 15% egg yolk in D-PBS): L(pg)[cpa] = 1.7 x 10(-15) m(3)/Ns (0.01 microm/min-atm) and E(Lp)[cpa] = 122.2 kJ/mole (29.2 kcal/mole) (R(2) = 0.98); and in "high" CPA media (consisting of 4% glycerol, 16% raffinose, and 15% egg yolk in D-PBS): L(pg)[cpa] = 0.68 x 10(-15) m(3)/Ns (0.004 microm/min-atm) and E(Lp)[cpa] = 63.6 kJ/mole (15.2 kcal/mole) (R(2) = 0.99). These parameters are significantly different than previously published parameters for mammalian sperm obtained at suprazero temperatures and at subzero temperatures in the absence of extracellular ice. The parameters obtained in this study also suggest that damaging intracellular ice formation (IIF) could occur in mouse sperm cells at cooling rates as low as 25-45 degrees C/min, depending on the concentrations of the CPAs. This may help to explain the discrepancy between the empirically determined optimal cryopreservation cooling rates, 10-40 degrees C/min, and the numerically predicted optimal cooling rates, greater than 5000 degrees C/min, obtained using suprazero mouse sperm permeability parameters that do not account for the presence of extracellular ice. As an independent test of this prediction, the percentages of viable and motile sperm cells were obtained after freezing at two different cooling rates ("slow" or 5 degrees C/min; "fast," or 20 degrees C/min) in both the low and high CPA media. The greatest sperm motility and viability was found with the low CPA media under fast (20 degrees C/min) cooling conditions.  (+info)

*Hepatocyte

"Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes". Biotechnology and Bioengineering ... in which calcium is removed to disrupt cell-cell tight junctions by the use of a calcium chelating agent. Next, a solution ...

*List of MeSH codes (D16)

... cariostatic agents MeSH D27.505.696.706.320 --- cryoprotective agents MeSH D27.505.696.706.548 --- neuroprotective agents MeSH ... cardiotonic agents MeSH D27.720.799.113 --- cariostatic agents MeSH D27.720.799.180 --- cryoprotective agents MeSH D27.720. ... anti-allergic agents MeSH D27.505.954.122 --- anti-infective agents MeSH D27.505.954.122.085 --- anti-bacterial agents MeSH ... antiviral agents MeSH D27.505.954.122.388.077 --- anti-retroviral agents MeSH D27.505.954.122.388.077.088 --- anti-hiv agents ...

*Fixation (histology)

Small pieces of tissue (5×5×3mm) are placed in a cryoprotective embedding medium-OCT, TBS, or cryogel-then snap frozen in ... Antimicrobial Agents and Chemotherapy. 44 (8): 2086-2092. doi:10.1128/AAC.44.8.2086-2092.2000. PMC 90018 . PMID 10898680. http ...

*Cryobiology

Slow cooling methods rely on the fact that cells contain few nucleating agents, but contain naturally occurring vitrifying ... cryoprotective compounds, medical applications of reduced temperature, cryosurgery, hypothermia, and perfusion of organs). Cryo ...
Vitrification is a promising approach for cryopreservation of adherent cells because it allows complete avoidance of ice formation. However, high cryoprotectant (CPA) concentrations are required to prevent freezing, and exposure to high CPA concentrations increases the risk of osmotic and toxic damage. Although cell membrane transport modeling can be used for rational design of CPA equilibration procedures, the necessary permeability data is extremely scarce for adherent cells. This study validates a method for in situ measurement of water and CPA permeability in adherent cells based on the fluorescence quenching of intracellular calcein. Permeability parameters for endothelial monolayers were measured during exposure to four common cryoprotectants (dimethyl sulfoxide, ethylene glycol, propylene glycol and glycerol) at temperatures of 4°C, 21°C and 37°C. Propylene glycol exhibited the highest permeability and glycerol the lowest. The data was fit using an Arrhenius model, yielding activation ...
Cryoprotective Efficiency of Medium Combining Non-Penetrating and Penetrating Cryoprotectants When Freezing Erythrocyte Suspensions of Various Volumes
Microbial communities enriched from diverse environments have shown considerable promise for the targeted discovery of microorganisms and enzymes for bioconversion of lignocellulose to liquid fuels. While preservation of microbial communities is important for commercialization and research, few studies have examined storage conditions ideal for preservation. The goal of this study was to evaluate the impact of preservation method on composition of microbial communities enriched on switchgrass before and after storage. The enrichments were completed in a high-solid and aerobic environment at 55 °C. Community composition was examined for each enrichment to determine when a stable community was achieved. Preservation methods included cryopreservation with the cryoprotective agents DMSO and glycerol, and cryopreservation without cryoprotective agents. Revived communities were examined for their ability to decompose switchgrass under high-solid and thermophilic conditions. High-throughput 16S rRNA ...
Before freezing, embryos are first equilibrated in special solutions containing cryoprotecting agents. These agents protect the embryos from intracellular ice formation, which would be detrimental to their viability. The embryos are then placed in special straws, sealed and put in a machine with an integrated computer. This machine, the programmable freezer, lowers the temperature in a slow controlled manner until -196oC. There are several protocols of slow freezing, depending on the stage of the embryos and the type of cryoprotectant solutions used. The embryos are then plunged in liquid nitrogen and are stored until used.. Usually 1-3 embryos are placed in each straw. In this state, embryos may be preserved for a very long period of time. Embryos can be frozen at the 2PN stage (Day 1), cleavage stage (2-8 cells; Days 2-3), or blastocyst stage (Days 5-6 post oocyte retrieval).. Cryopreservation in liquid nitrogen at a temperature of -196oC does not require electric power. The only requirement ...
On Tue, 6 Jul 1999, jim wrote much, including: , Now to your point: A cryoprotectant which only surrounds and not infiltrates , the specimen can only be useful for the second method, since such a medium , contributes to the bulk of the specimen and does not lower the freezing point , of the specimen itself. Obviously this is true. I dont think even the makers of OCT compound and other such goo would claim a cryoprotective action. These materials serve to glue the frozen specimen to the cryostat chuck or bit of cork, and also provide some protection against sublimation of the ice (freeze drying) in stored frozen specimens. Gelatin (approx 2%) has a similar consistency and does the same job more cheaply. , To be effective during vitrification the cryoprotectant , must infiltrate the specimen. It must indeed. A.G.E.Pearse (Histochemistry Vol 1) makes the point that most of the cryoprotectives used with tissues - sucrose being the most popular one - penetrate only the extracellular spaces. He ...
... on the pregnancy rate of the vitrified-thawed Formosan sambar deer embryos. Hsin-Hung Lin, Chih-Hua Wang, Shann-Ren Kang, Chin-Hui Tseng, Mu-Jung Cheng, Wen-Lin Song, Ting-Chieh Kang, Shyh-Shyan Liu, and Perng-Chih Shen. The aim of this study was to investigate the effect of cryoprotectant components on the pregnancy rate of the vitrified-thawed Formosan Sambar deer blastocysts. The does were implanted with CIDR for 12 days and superovulated by intramuscular injection with eCG at day 10. The 4- to 8-cell stage embryos were collected by flushing the oviduct through midventral laparotomy at day 4 after mating. After collection, these embryos were subsequently cultured in the synthetic oviduct fluid solution (SOF) medium. Results showed that the blastocyst rate of cultured embryos was 29.6%. Thereafter, the blastocysts were vitrified with freeze medium containing 16.5% EG plus 16.5% DMSO (A) and 6.8 M EG (B), respectively. The pregnancy rate of vitrified ...
You might find some useful information in The Zebrafish Book, Chapter 7: http://zfin.org/zf_info/zfbook/cont.html#cont7 ======= Carrie R. Jones Zebrafish International Resource Center 5274 University of Oregon Eugene, OR 97403-5274 Phone: (541) 346-6028 ext. 22 Fax: (541) 346-6151 Email: cjones at zfin.org ======= May-Su YOU wrote: , I am Maysu You, now working at IMCB (Institute of Molecular and Cell , Biology) in Singapore. We are now working on sperm , squeezing,freezing and in vitro fertilization of Zebrafish. We got , around 90% of IVF successful rate when we used fresh sperm (from , both squeezed sperm and dissected testes) . So we moved to freezing , step. We tried two different cryoprotectants (methanol and BSMIS: , Buffered sperm motality inhibitor solution). After thawing, we , cannt get any IVF embryo. Therefore, we doubt about the freezing , procedure which we used at the moment. We also tried to observe the , sperm, but even with trypan blue, we are not sure wheather the , sperm ...
Cryoprotective solutions have been supplemented with macromolecules such as sucrose and human serum albumin (HSA). Several studies have shown that such molecules help to reduce physical damage and help to maintain osmotic pressure of the extracellular fluid (Shaw, et al. 2000). Besides its cryoprotective role, HSA facilitates gamete or embryo manipulation by preventing adsorption to the surface of petri dishes and pipettes through saturation of the potential binding sites. Also, the increased viscosity of the media, caused by the addition of HSA, promotes the ease of embryo handling and manipulation (Trounson and Gardner 2000 ...
Cryobiology: International Journal of Low Temperature Biology and Medicine is the official journal of the Society for Cryobiology.. It is published bi-monthly by Elsevier and contains research articles on all aspects of low temperature biology and medicine including:. ...
Dear Patsy, Your cryoprotectant solution sounds suspiciously like the solution we use to store vibratomed sections at -20C to actually keep them FROM freezing. It allows us to keep them very cold but without the damage of actually freezing them. The only difference is that we add glycerine to the solution. I suspect the ethylene glycol is the problem, I dont think it will allow the tissue to freeze. Try leaving it out of the mix and just make a 30% sucrose/0.1M phosphate buffered solution. Just my thoughts. Take care, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: [email protected] Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Patsy Ruegg Sent: Friday, January 12, 2007 12:54 PM To: histonet Subject: ...
Cryobiology: International Journal of Low Temperature Biology and Medicine publishes research articles on all aspects of low temperature biology and...
Audrey U. Smith, circa 1960s Audrey U. Smith (1915-1981), the mother of Cryobiology, was born in India on 21 May 1915. She was educated Kings College, London (first-class B.Sc., 1935); Bedford College for Women (first-class B.Sc. in physiology, 1936); registered … Continue reading →. ...
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Excellent freezing of the specimens intended for examination in the electron microscope is one of the most important prerequisites for achieving reproducible results from the various subsequent cryopreparation methods.. The freezing method should produce microcrystalline or amorphous ice from the specimen water. To achieve this, the specimens must be frozen as quickly as possible at a freezing rate no lower than 10 000°C/s.. The conventional freezing methods in use are plunge freezing, jet spray and cold block (slamming) cryo fixation. However, due to the poor heat conductance of water, these methods can only satisfactorily freeze specimens measuring up to between 10 and 20µm.. Thicker specimens (such as tissue samples) could only be frozen in the past, if a cryoprotectant was added to lower the freezing point of the water in the specimen. The disadvantage of chemical cryoprotectants is that they often affect certain cell structures, causing different types of undesirable artefacts.. By using ...
On the strategy of reimbursing after the years premiums are paid, I also believe thats a crap shoot. It may not have been challenged yet under audit (Ive not heard of any cases), maybe because it hasnt been found (after all you have personal checks to vouch you paid personally, right?) If it gets caught AFTER one becomes disabled....that annual benefit will be a HUGE incentive for the IRS to attack that strategy. Thats a headache I dont want if my family is relying on ALL that tax free income ...
A novel model capable of quantitatively describing and predicting Intracellular Ice Formation (IIF) as a function of temperature in a cell population during the cooling stage of a cryopreservation protocol, without Cryo-Protective Agent (CPA) is proposed. The model accounts for water osmosis and IIF occurrence during freezing of the cell population, whose size distribution dynamics is simulated by means of a suitable population balance approach. It is found that IIF temperature depends upon the cell size, i.e. it is higher for larger cells. Correspondingly, the Probability of IIF (PIIF) results to be dependent on the initial size distribution of the cell population. Model reliability is successfully verified by predicting experimental data available in the literature of PIIF at different, constant cooling rates with better accuracy as compared to previous theoretical approaches.. ...
Populations of Redside Dace Clinostomus elongatus have declined in many areas across the speciesNorth American range. Therefore, the development of sperm cryopreservation technology would provide an invaluable means of preserving genetic diversity in populations that are in imminent danger of extirpation.We developed cryopreservation protocols by testing the effects of diluent (buffered sperm motility-inhibiting saline solution [BSMIS]; BSMIS + glycine; sucrose; and Hanks balanced salt solution [HBSS]), cryoprotectant (dimethyl sulfoxide [DMSO]; propylene glycol [PG]; N,N-dimethylacetamide [DMA]; and methanol), freezing rate (1, 5, and 10◦C/min), and male-to-male variation on sperm quality. Incubating sperm in extenders affected motility;BSMIS + glycine + methanol,BSMIS + glycine + PG, and HBSS + methanol were the only treatments for which motility was not significantly different from that of fresh sperm. Sperm frozen with sucrose had higher motility than sperm frozen with BSMIS + glycine, ...
Hepatocytes are widely used in the pharmaceutical and medical fields for drug metabolism studies, bioartificial liver devices, and repopulation of damaged livers as an alternative to transplantation. However, these cells are scarce and difficult to maintain in culture for prolonged periods of time. Banks of cryopreserved liver cells would significantly alleviate issues of hepatocyte availability, and efforts are being made to improve the viability and functionality of frozen hepatocytes. Previously, most work on improving post-thaw viability has hinged on limiting the physical damage of freezing by adding cryoprotective agents and optimizing cooling rates. Membrane-permeable cryoprotectants, such as dimethyl sulfoxide, though widely used, can be extremely toxic to the cell. More natural, non-membrane-permeable cryoprotectants, inspired by freeze-tolerant animals have also been used. A non-metabolizable glucose analog, 3-0-methyl- glucose (30MG), has shown promise with hepatocytes and was used in ...
Jenderek, M.M.; Ambruzs, B.; Tanner, J.; Holman, G.; Ledbetter, C.; Postman, J.; Ellis, D.; Leslie, C. 2014. Extending the dormant bud cryopreservation method to new tree species. In: Reed, B.M. (ed). Proceedings of the International Conference. 2. International Symposium on Plant Cryopreservation. Fort Collins (USA). 11-14 Aug 2013. Conference Paper International Society for Horticultural Science (ISHS). ISBN 978-94-62610-27-9. pp. 133-136. Acta Horticulturae. ISSN 0567-7572. no.1039 ...
Oocyte Cryopreservation abroad in India offers info on cost Oocyte Cryopreservation India,Oocyte Cryopreservation IVF Doctors &hospitals India.
I15 Should Cryoprotectants be Formulated According to the Mouse Genome?. Mike Legge, Mat Byers and Stephen Bird. Molecular Embryology Group, Department of Biochemistry, University of Otago, PO BOX 56 Dunedin, New Zealand. The use of penetrating cryporotectants such as dimethyl sulphoxide and I,2-propanediol is well established for mouse embryo cryopreservation. However, the sensitivity of both oocytes and embryos to adverse effects of both cryoprotectants and the freezing process is well documented (Duliquost et al. 1999). For mouse genome cryopreservation this is especially important as there is a correlation between mouse embryo genotype and cryopreservation success. (Schmidt et al. 1985). With the increasing necessity to archive mouse genomes by embryo and gamete cryopreservation the improvement in cryoprotectant formulations is becoming increasingly important. Recently we identified the non-enzymatic formation of formaldehyde in cryoprotectant solutions and that at the micro-molar ...
The use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential ...
Development of effective cryopreservation protocols will be essential to realizing the potential for clinical application of neural stem and progenitor cells. Current cryopreservation protocols have been largely employed in research, which does not require as stringent consideration of viability and sterility. Therefore, these protocols involve the use of serum and protein additives, which can potentially introduce contaminants, and slow cooling with DMSO/glycerol-based cryopreservation solutions, which impairs cell survival. We investigated whether serum- and protein-free vitrification is effective for functional cryopreservation of neurosphere cultures of neural stem or progenitor cells. To protect the samples from introduction of other contaminants during handling and cryostorage, an original "straw-in-straw" method (250 l sterile straw placed in 500 l straw) for direct immersion into liquid nitrogen and storing the samples was also introduced. The protocol employed brief step-wise ...
View details for Studies on the effect of certain supplements in cryoprotective medium on zebrafish (Danio rerio) oocytes quality after controlled slow cooling..
Background Simple and effective cryopreservation of human oocytes would have an enormous impact on the financial and ethical constraints of human assisted reproduction. Recently, studies have demonstrated the potential for cryopreservation in an ice-free glassy state by equilibrating oocytes with high concentrations of cryoprotectants (CPAs) and rapidly cooling to liquid nitrogen temperatures. A major difficulty with this approach is that the high concentrations required for the avoidance of crystal formation (vitrification) also increase the risk of osmotic and toxic damage. We recently described a mathematical optimization approach for designing CPA equilibration procedures that avoid osmotic damage and minimize toxicity, and we presented optimized procedures for human oocytes involving continuous changes in solution composition. Methods Here we adapt and refine our previous algorithm to predict piecewise-constant changes in extracellular solution concentrations in order to make the predicted ...
CryoSearch was created by BioCision in order to help standardize the field of cryopreservation through the sharing of protocols and encouraging discussion about protocols and methods. At BioCision, our mission is to standardize sample handling. While our products help do that, products are only one part of reducing variability. The other part is how those products get used; in other words, protocols. Since we have somewhat of a specialty in cryopreservation, and there arent any other resources like CryoSearch, we thought this would be a great place to start.. Like the site? Wed love for you to check out our cryopreservation products as well. We have leak-proof and barcoded cryovials, a line of containers for slow, controlled-rate cell freezing, and tube racks that ensure precise temperature control and can also be used for snap-freezing as they are liquid-nitrogen compatible. We have a bunch of other products as well, all designed to help make your science more reliable.. Even if youre not in ...
Cryobiology is the branch of biology that studies the effects of low temperatures on living things within Earths cryosphere or in science. The word cryobiology is derived from the Greek words κρῧος [kryos], "cold", βίος [bios], "life", and λόγος [logos], "word" (hence science). In practice, cryobiology is the study of biological material or systems at temperatures below normal. Materials or systems studied may include proteins, cells, tissues, organs, or whole organisms. Temperatures may range from moderately hypothermic conditions to cryogenic temperatures. At least six major areas of cryobiology can be identified: 1) study of cold-adaptation of microorganisms, plants (cold hardiness), and animals, both invertebrates and vertebrates (including hibernation), 2) cryopreservation of cells, tissues, gametes, and embryos of animal and human origin for (medical) purposes of long-term storage by cooling to temperatures below the freezing point of water. This usually requires the ...
Embryo cryopreservation involves the generation of pre-implantation embryos using males provided by the investigator and the controlled rate cooling of collected embryos to -30°C followed by rapid freezing of embryos in liquid nitrogen to -196°C.. While embryo cryopreservation is the gold standard for preserving mouse germplasm, not all mouse strains are suitable for embryo cryopreservation. However, we have been successful at cryopreserving embryos from several different mouse strains.. Embryos for cryopreservation can be obtained in two basic ways: ...
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
In the past two decades, laboratories around the world have produced thousands of mutant, transgenic, and wild-type zebrafish lines for biomedical research. Although slow-freezing cryopreservation of zebrafish sperm has been available for 30 years, current protocols lack standardization and yield inconsistent post-thaw fertilization rates. Cell cryopreservation cannot be improved without basic physiological knowledge, which was lacking for zebrafish sperm. The first goal was to define basic cryobiological values for wild-type zebrafish sperm and to evaluate how modern physiological methods could aid in developing improved cryopreservation protocols. Coulter counting methods measured an osmotically inactive water fraction (Vb) of 0.37+/-0.02 (SEM), an isosmotic cell volume (V(o)) of 12.1+/-0.2mum(3) (SEM), a water permeability (L(p)) in 10% dimethyl sulfoxide of 0.021+/-0.001(SEM)mum/min/atm, and a cryoprotectant permeability (P(s)) of 0.10+/-0.01 (SEM)x10(-3)cm/min. Fourier transform infrared ...
There are many benefits of oocyte and embryo cryopreservation. When used as part of the IVF treatment process, cryopreservation can increase the likelihood of achieving pregnancy for many patients. Cryopreservation also allows couples to continue to see their families grow as IVF cycles can be repeated, years later, from the same group of eggs originally recovered for treatment.. During an IVF cycle, between 10 and 30 eggs can typically be recovered from the ovary. While it is likely that not all of these eggs will be deemed appropriate for cryopreservation, the process frequently allows couples to save a number of eggs for multiple conception attempts. In some cases, this can lead to successful treatment without the use of fertility drugs. It also reduces the likelihood that another egg retrieval procedure will be required. The number of embryos transferred depends on the number of eggs available, age, and other factors that are unique to each patient. In some cases, cryopreservation makes ...
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A device for use in cryopreservation of blood vessels comprising a pair of styles insertable into the ends of a dissected blood vessel segment. The styles are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant.
Citation: Jenderek, M.M., Ambruzs, B.D., Holman, G.E., Skogerboe, D.M., Staats, E.R., Turner, M., Ellis, D.D. 2007. Germplasm Preservation of Vegetatively-propagated Crops at the National Center for Genetic Resources Preservation. American Society for Horticultural Science, HortScience. July 16-19, 2007.Scottsdale, Arizona. 42:962. Meeting Abstract. Interpretive Summary: Out of 476,049 germpaslm accessions maintained by the USDA, ARS, National Plant Germplasm System (NPGS), ca. 30,000 are vegetaively-propagated and as such require preservation as non seed propagules. Numerous research reports demonstrated the advantages of long term storage of plant tissues in liquid nitrogen over field only maintained collections. While successful cryopreservation protocols were established for many plant species, the protocols are usually not applicable for the entire collection of a species or genus they were developed, and have to be modified for accessions not responding to the procedure. Currently, the ...
1. Perrotti M, Badger WJ, McLeod D et al. Does laparo-scopy beget underuse of partial nephrectomy for T(1) renal masses? Competing treatment decision pathways may influence utilization. J Endourol 2007; 21(10): 1223-1128. 2. Phillips B, Ball C, Sackett D et al. Levels of evidence and grades of recommendation. Oxford Centre for Evi-dence based Medicine. Downloaded at http://www. cebm.net/levels_of_evidence.asp. 3. Acker JP, Larese A, Yang H et al. Intracellular ice formation is affected by cell interactions. Cryobiology 1999; 38(4): 363-371. 4. Hoffmann NE, Bischof JC. The cryobiology of cryo-surgical injury. Urology 2002; 60 (2 Suppl 1): 40-49. 5. Baust JG, Gage AA. The molecular basis of cryo-sur-gery. BJU Int 2005; 95(9): 1187-1191. 6. Mouraviev V, Joniau S, Van Poppel H et al. Current status of minimally invasive ablative techniques in the treatment of small renal tumors. Eur Urol 2007; 51(2): 328-336. 7. Woolley ML, Schulsinger DA, Durand DB et al. Effect of freezing parameters (freeze cycle ...
So does it really make sense to preserve the body of a person if, for some reason, perfusion has not been done? Lets imagine that the deceased person was taken without any perfusion and placed in regular water ice or in dry ice and then sent, for example, by plane from the USA to Russia, after which that person was cooled to the temperature of dry ice and then even further to -196°C.. To understand the situation, we need to return to the past, to the early days of the cryonics history. Its history began in the 1960-s when the book "The Prospects of Immortality" by Robert Ettinger was published. I hope everyone reading this have read the book by "the father of cryonics", because its really accessible and contains just a minimum of technical and biomedical details. The book focuses more on moral and philosophical questions, arguing for the benefits of cryonics, while raising questions about why it should be done and how it will be done in the future, how is it compatible with religion.... This ...
This advisory is written for those who would like to understand the processes of mouse strain cryopreservation, why it is important and how to take advantage of the services offered. The most efficient method to protect mouse strains is by cryopreservation of gametes or embryos. Cryopreservation, and especially re-animation from frozen material, is not a trivial exercise, nor is the establishment of a cryopreservation and IVF laboratory cheap. There are numerous laboratories around the world that offer these services.
Recently, Ive heard of something called Oocyte cryopreservation, where a (fertilized, I think) egg from a woman is extracted, frozen and later thawed and reinserted into the woman to delay pregnancy.. Now, this is just an idea, I dont know if this is actually possible, but can this frozen egg be implanted into a different woman, who isnt the original owner of the egg? If yes, whose genes would the child inherit? Would it get genes from all three parents, or just from the original owner of the sperm and egg?. ...
Speaking of brains. Currently, among other things, I develop software for viewing serial block-face microscopy data, on a contract. This is my private opinion, of course - and I am not a neurologist, my main specialization is graphics, I look at neurons to tune and test the software, I dont quite know what all the little bits around are - I look at a bit and Im like, what is it? And then I go to re-read description by one of other people at the project, and I am like, ohh, I think its a mitochondrion inside a dendrite. And then I wonder - why is it here? What does it matter where it is? What is this thing that connects it to the wall? Is it some weird imaging artifact? I do not claim to speak for everyone. Im doing my part which, among other things, can help to figure out how to preserve brains or how to digitize them ...
Techniques of controlled-rate freezing are utilized that slowly cool embryos in cryoprotectant fluid ("anti-freeze" solution) from body temperature down to -196°C, at which temperature they are stored in containers of liquid nitrogen called dewars. The embryos are actually contained within special indelibly labeled plastic vials, or straws, that are sealed prior to freezing. Once frozen, they are placed inside labeled tubes attached to aluminum canes and stored in numbered canisters within the liquid nitrogen dewar. Site and label designations are stored in three separate file systems to avoid confusion and misidentification of cryopreserved embryos. When it comes time to thaw the embryos, all available identifiers of the stored specimen must match and be confirmed before thawing commences. The embryos are thawed out at room temperature, which takes about one to two minutes. However, the most critical element of the thaw procedure is not the timing but the careful dilution of the cryoprotectant ...
Is the exposure time of bovine embryos to ethylene glycol (EG) prior to freezing and/or after thawing critical to survival?. John F. Hasler. Efficient and efficacious cryopreservation of bovine embryos is critical to the commercial ET industry because, as shown by the most recent AETA statistics (2011), 72% of embryos were frozen following collection versus only 28% that were transferred fresh into recipients. Following the published report of Voelkel and Hu in 1992 on cryopreservation with EG, the commercial bovine ET industry rather quickly switched from glycerol to EG as the major cryoprotectant in freezing media. The overall percentage of embryos frozen in EG rose rapidly starting in 1992 and reached 97% in 2008, the last year that the AETA collected data on this specific statistic.. During the past 20 years there has been a continuing debate among ET practitioners regarding the question of whether EG is more toxic than glycerol to bovine embryos. This concern has led some practitioners to ...
Stem cells are the basic cells which have the capacity to develop into almost all types of cells of the body. If given correct environment and necessary stimulus, each stem cell can develop into a specialized tissue/organ. Stem cells can be stored or cryopreserved for years using cryopreservation methods.
From: Mike Darwin <. Date: 31 May 95 00:03:14 EDT Subject: SCI.CRYONICS BPI Tech Brief 16: Canine Brain Cryopreservation A Brief Lay-Level Summary of Biopreservations Canine Brain Cryopreservation Results by Charles Platt In the 1950s, experiments showed that the damage caused when the cells of a mammal are frozen can be reduced if the cells are first treated with a solution of glycerol. More recently, work by Leaf, Darwin, et. al. suggested that damage to cryonics patients might be further minimized if perfusion with glycerol was carefully monitored and controlled, using a solution whose concentration gradually increased during the perfusion process to a very high concentration where much less ice will form than is the case when no cryoprotectant or lower levels of cryoprotectant are used. Until now, there has been no systematic study to verify that this kind of controlled perfusion of cryonics patients really does result in less freezing damage than a simpler protocol. In particular, no ...
Date: 19 Mar 96 00:18:15 EST From: Mike Darwin ,. , Subject: BPI TECH BRIEF #18 Cryopreservation of CryoCare Patient #C-2150 by Mike Darwin Introduction On December 12th, 1995 James Gallagher, a 55-year-old software developer from Sunset Beach, California, became CryoCares first member to enter cryopreservation. He also became the first patient ever to benefit from new technologies developed to reduce three forms of injury: * pre-mortem shock * warm ischemia (the time interval between pronouncement of death and restoration of adequate blood circulation) * cold ischemia experienced during initial blood washout and cooling, and also during iced-transport from the location where legal death occurred to the facility where cryoprotective perfusion is carried out. The following is not quite a full technical report, but neither is it simply a lay-level of summary of key events without reference to the technical details and the impact those details had upon this patients care and potentially, future ...
One of the critical elements for the clinical availability of cells for use in immunotherapy is cryopreservation. However, cryopreserving cells relies on antiquated protocols which raise serious safety concerns. DMSO has been associated with post-thaw morphological and epigenetic changes, weakened biodistribution and acute toxicity ...
Efficient cryopreservation of stem cells is essential for the maintenance of consistent stem cell stocks. Many of the existing formulations of cryopreservation media rely on high percentages of poorly defined serum or albumin.
Fifty years ago, scientists performed the first-ever cryopreservation procedure on a recently-deceased human patient: James Bedford, who had died of pancreatic cancer earlier that day. As per his will, his body was frozen in liquid nitrogen within hours of his clinical death. Kept at a temperature of -196 degrees Celsius for five decades, Bedfords hope was that he could be resuscitated in the future, at a time when our technological advancements had brought about the cure for cancer and... Death itself. January 12th is celebrated by proponents of cryopreservation as Bedford Day . This year is a particularly significant milestone: since Bedford was frozen in 1967, this marks a half century since his death and subsequent preservation. Unfortunately for him, the envisioned future in which cryopreserved corpses can be brought back from the dead-and subsequently the past-has yet to materialize.
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... of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use.
IV. EFFECTS OF CRYOPRESERVATION ON THE HISTOLOGY OF SELECTED TISSUES (Liver and Kidneys) Histology was evaluated in two animals each from the FIG and FIGP groups, and in one control animal. Only brain histology was evaluated in the straight-frozen control … Continue reading →. ...
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The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for future use.
Dr. Rita Bakshi offers cryopreservation, sperm freezing, embryo freezing, ovarian tissue freezing centre, clinic in Delhi, India at very low cost.
The Society For Venturism has received the remaining $28,000 funding needed for cryopreservation of ALS patient Aaron Winborn at Cryonics Institute, according
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DISCUSSION AND CONCLUSIONS In considering the meaning of the observations reported here, we will discuss the following three general questions. 1) What can be said to have learned from the observations reported here? 2) Are our observations consistent with, and are they illuminated by, cryobiological findings obtained on simpler systems (particularly whole organs)? And finally, 3) what are the implications of our findings with respect to the repair of freezing damage in cryopreserved humans and the feasibility of cryonics in general? 1. What have we learned from the present investigation? Caveats. With respect to this question, it is first important to define the limitations of this study. First, the observations were made on a single patient only, and could theoretically be unique to this patient, making our observations no better than "anecdotal." Second, we do not know what the tissue levels of glycerol were in the areas subjected to investigation, so it is difficult to estimate the amount of ...
By Natasha Vita-More First published in Cryonics Magazine "If the aging process is controlled in a similar way in worms and humans, then we can use what we learn about worms to speed our study of higher organisms." - Cynthia Kenyon Preserving memory after cryonic preservation is a breakthrough science for cryonics, which has been a huge hurdle for cryonics. The research leading to this breakthroug.... Read More » ...
Before we begin, a one question quiz: What is your practice overhead? Is it 60%? Maybe 70%? As low as 40%? That would be spectacular. If you dont have your overhead numbers pinned down, its time for a meeting with your CPA. According to the Dental Sales Academy, in 1999, average dental practice overhead was at 58% of revenue. When we fast forward to 2011, it jumps to a steep 69%. If your overhead is looming in that ballpark or worse, its time to think, what is my blueprint for 2013 to bring that number back to earth ...
Have a seat, business executives, and listen to one of the more eye-opening stats Ive seen in a long time: More than 60 percent of employees surveyed in a…
Before this technology can be used in human preservation the perfusion rates and the levels of anti-coagulant will need to be optimised for different sized ovaries and of course trials on the normality of offspring will be required. Additionally it could also perhaps be applied to the cryo-preservation of other organs and even one day major ones such as kidneys where there are considerable difficulties in storing donated tissue. Although an organ, the ovary is small - though still with multiple cell types and a separate blood supply. So far attempts to freeze and store larger whole organs such as the heart, liver and kidney have proved unsuccessful. The primary challenge with whole organ cryo-preservation is that of preserving an intact vascular system and the Nottingham work, funded by the UKs Medical Research Council - is a major step in the right direction ...
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CryoStor™CS10 is an animal protein-free, serum-free cryopreservation medium containing 10% DMSO to prepare & preserve cells in ultra low temperatures.
The peculiarities of development of hypertonic cryohemolysis of human erythrocytes at cooling from 37 down to 0°Сunder the influence of different cryoprotectors and aluminum chloride were investigated. It was istablished, that the presence of PEG-1500 and also aluminum chloride in the medium noticeably enhanced the stability of cells to temperature-osmotic effects. The use of penetrating cryoprotectors leads to increasing of hypertonic cryohemolysis level at the second stage of incubation in hypertonic conditions.. ...
Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to Show moreCryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to assess the ...
The summer flounder, Paralichthys dentatus L., is a high-value species and considerable research has been conducted to determine practices conducive for its culture. As milt can be limited in this species, experiments were conducted to develop a practical sperm cryopreservation protocol for hatchery use. Two dilution ratios (1:2 and 1:4; sperm:extender), 2 diluents (saline and sucrose-based), 2 cryoprotectants (10% DMSO and 12% glycerol) and 3 freezing rates (?5, ?10 and ?15°C min?1) were evaluated using differential staining to assess post-thaw sperm survival. Seven combinations of the factors examined reduced post-thaw viability by less than 30%. The average viability of sperm from fresh, pooled flounder milt (67.2 ± 2.9%) was not different from that of thawed milt diluted 1:4 with sucrose diluent (10% DMSO) frozen at ?5°C min?1 (38.4 ± 7.7%) and fertilization and hatch success were not different in trials using fresh or thawed, cryopreserved sperm. From these experiments a practical
Vitrification is an ultra‐rapid cryopreservation technique that provides excellent survivability by using optimal concentrations of cryoprotectants used in a step‐wise process supporting rapid dehydration of the oocyte. The dehydrated oocyte is loaded into a thin storage device that will facilitate ultra‐rapid cooling of the oocyte when plunged into liquid nitrogen - eliminating concerns of damaging ice‐crystal formation associated with traditional slow‐freezing procedures. Thaw solutions are formulated to support rehydration through the rapid warming process that follows vitrification to optimize cellular survival.. Irvine Scientifics vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. These solutions support implantation and pregnancy rates comparable to those of fresh cycles.. ...
Taconic offers both embryo and sperm cryopreservation. Taconic also offers revitalization of cryopreserved lines. Learn more today.
There is a lump sum due at the time of cryopreservation. This pays for the cost of transportation, cryopreservation protocols, and also for ongoing costs of maintenance for the cryonics patient. This lump sum is normally covered with a dedicated life insurance policy.. Cryonics organizations also have membership dues, which are separate from the cost of the cryonics life insurance. There are no additional costs due once a member is in cryopreservation.. ...
Induct yourself into a one-on-one training to develop knowledge and hands-on skills in all aspects of Cryotech vitrification of human oocytes and embryos. Key expert tips & tricks to achieve 100% survival gained will be imparted. At the end of the course, the candidates will be confident to achieve near 100% survival for eggs and embryos in their laboratories.. Course Objectives. During the course each participant receives one-on-one training to :. ...
Some key ideas, and experimental finding concerning the probability that crystallization of a liquid or its binary solutions will occur at moderate cooling rates are discussed, with emphasis on the case of aqueous solutions. The use of cryoprotectants and of pressure to dimimish these probabilities, hence to promote vitrification, is rationalized. Some new data on crystallization of bulk and emulsified aqueous solutions of the cryoprotectant glycerol are presented to illustrate the principles. (Keywords:) crystallization, cryproltection, nucleation, vitrification.*NUCLEATION
Morphological changes in equine, bovine, and canine erythrocytes, when interacting with 20% dimethylsulfoxide (DMSO) and 30% polyethylene oxide with molecular mass of 1500 (PEO-1500) solutions, as well as after cell cryopreservation at the presence of the mentioned cryoprotectants have been studied in this research using light microscopy. Frozen-thawed erythrocytes of all animal species were established to be transformed into echinocytes under DMSO penetrative cryoprotectant effect and into stomatocytes under a non-penetrative one. The state of animal frozen-thawed erythrocytes when transferring them into physiological conditions was analysed.. ...
Tobacco BY-2 suspension cells were successfully cryopreserved by a vitrification method combined with an encapsulation technique. Cell cultures cryopreserved using the optimal conditions established in this study could be thawed and grown enough to subculture in fresh medium within 14 days. However, the vitrification method was less effective for cryopreservation of BY-2 than a simplified slow prefreezing method.. ...
Looking for Vitrification? Find out information about Vitrification. Heat treatment of a material such as a ceramic to produce a glazed surface. Formation of a glassy or noncrystalline material. An experimental procedure for... Explanation of Vitrification
On July 5, 2016, the Federal Circuit vacated and remanded a district court decision holding that U.S. Patent No. 7,604,929, which is directed to a…
Brain tissue was obtained from eight CTB-injected rats (6-12 d after tracer injection) and 10 uninjected rats that had been deeply anesthetized with pentobarbital and perfused transcardially with 100 ml of PBS followed by 500 ml of 4% paraformaldehyde in 100 mm phosphate buffer. After postfixation in the same fixative for 24-48 h, series of coronal sections (30 μm) were cut using a vibrating microtome and stored in cryoprotectant solution at −20°C for up to 2 weeks as described previously (Rosin et al., 2006). Sections were incubated free-floating in primary antibodies in 0.1 m Tris-buffered saline, pH 7.4, with 10% horse serum and 0.1% Triton X-100 for 24 h at 4°C unless otherwise noted. CTB was detected using a rabbit antibody (1:1000, 48 h incubation; Sigma-Aldrich, St. Louis, MO; #C3062) followed by a Cy3-tagged donkey anti-rabbit IgG (1:200; Jackson ImmunoResearch, West Grove, PA); this rabbit anti-CTB antibody gives the same pattern of labeling as a goat anti-CTB (List Biological ...
Video articles in JoVE about pregnancy outcome include Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface, Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts, Accurate and Simple Evaluation of Vascular Anastomoses in Monochorionic Placenta using Colored Dye, Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats, Co-Transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-Based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery, Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy, Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas.
For decades, fertility clinics utilized a slow freeze technique for freezing of eggs and embryos. This method was successful with embryos, but frozen eggs showed only a 50% survival rate. It was clear that a more efficient and successful technique was needed. Today, vitrification allows for 90% or greater survival for both embryos and eggs.
Vitrification is now the preferred choice for cryopreservation of human gametes and embryos.. Clinical outcomes obtained from vitrification normally exceed those from slow freezing - and reports of comparable pregnancy and implantation rates from fresh and vitrified cases are becoming more frequent. In addition, vitrification provides the possibility to stop at any time in the process if conditions are less than perfect.. ...
Vitrification is a newer technique which uses ultra-rapid cooling, together with a much higher concentration of cryoprotectants. Vitrification is used for cryopreservation of eggs and embryos ,
Introduction. Biology coursework Investigation- Affect of sucrose concentration on the rate of respiration. Planning Aim and Background information The aim of this investigation is to find out how the affect of changing the sucrose concentration affects the rate of respiration of yeast. The reaction can be measured by the amount of carbon dioxide given of by yeast and ethanol is also produced as a result of the reaction. Yeast (Saccharomyces cerevisiae) is a unicellular fungus, which is frequently used in baking. The precise classification is a field that uses the characteristics of the cell, ascospore and colony. Physiological characteristics are also used to identify species. One of the better-known characteristics is the ability to ferment sugars for the production of ethanol. Budding yeasts are true fungi of the phylum Ascomycetes, class Hemiascomycetes. The true yeasts are separated into one main order Saccharomycetales. Yeasts multiply as single cells that divide by budding (eg ...
The present Core C (Cryopreservation and Stem Cell Biology Core), evolved from a predecessor devoted strictly to cryopreservation. Core C is far more sophistica...
Thawing - should occur quickly and cells diluted in pre-warmed culture medium to prevent any toxic effects of cryoprotectants in super-zero temperatures. Incubate and examine (phase contrast) the next day. Cells may need to be washed in media if cryoprotectant has a known adverse cytopathic effect ...
CBS Isothermal Freezers feature a patented liquid Nitrogen jacket to provide uniform storage temperatures in the -190°C range. Request a Quote!
HART IVF Fertility Clinic formerly known as The North Houston Center for Reproductive Medicine (NHCRM) is an office based fertility program offering a wide assortment of diagnostic and therapeutic options for the treatment of infertility.
NextGen Add-On Transmitter for Remote Extender (AAA-TX433) This NextGen RF battery transmitter for remote extender is for the LRRX Invisible multi-room remote extender. This transmitter allows for additional remotes to control other home electronics near the RF receiving unit. Includes rechargeable battery, AA adapter sleeve, and transmitter. One transmitter/battery is required for each additional remote to convert to RF. Solid Signal is your source for the NextGen RF battery transmitter for remote extender. Key features For use with the LRRX Invisible multi-room remote extender Control A/V components up to 100+ feet Includes rechargeable battery, AA adapter sleeve, and transmitter
New York Times: Putting Their Eggs and Hopes on Ice - These Companies Really, Really, Really Want to Freeze Your Eggs. Egg-freezing clinics are aggressively courting a new generation. Younger millennials are heeding the call. Jennifer Lannon lay, her feet propped in stirrups, on an examining table at Extend Fertility, an egg-freezing clinic in Midtown Manhattan…. Read More ». ...
For more than 20 years, BroadcastMed has been innovating digital strategies for healthcare organizations. The company was first in the world to broadcast live surgeries on the internet using its ORLive solution which provides an intimate look inside the operating room.. ...
For the first time ever, a fish embryo survived being frozen and thawed, which could help preserve species and repopulate oceans. For decades, researchers have successfully cryopreserved - frozen and thawed - mammal embryos and a variety of animal sperm, but scientists were unable to do the same for fish embryos. Now, with a pairing of gold nanoparticles and lasers that thaw at millions of degrees per minute, University of Minnesota scientists successfully cryopreserved zebra fish embryos.
Automatic Fill Liquid Nitrogen Freezers for Cryo preservation and storage in cryogenic labs, medical facilities and research labs.
Caylor, R. E., Biesiot, P. M., Franks, J. S. (1994). Culture of Cobia (Rachycentron-canadum) - Cryopreservation of Sperm and Induced Spawning. Aquaculture, 125(41276), 81-92 ...
The efficacy of Triladyl®, a commercial cryomedium for bull semen, in the cryopreservation of both human and animal infective trypanosomes as compared to EDTA Saline Glucose (ESG) 10% glycerol was evaluated in the current ...
Effect of varying sucrose concentration on macrobehavioral aspects of licking in the rat.: Rats (eight male, eight female) were trained to lick 32% and 4% sucro
Press release - Market Insights Reports - Cryopreservation Equipment in Stem Cells Market 2019 Strategic Assessment - Thermo Fisher Scientific, Charter Medicals, praxair - published on openPR.com
The Report Cryopreservation Equipments in Stem Cells - A Global Market Watch, 2009-2015 provides information on pricing, market analysis, shares, forecast, and company profiles for key industry participants. - MarketResearchReports.biz
[The response of embryos of the Chinese mitten crab (Eriocheir sinensis) in terms of survival to cryoprotectants was investigated for basic knowledge to enhance cryopreservation success of E. sinensis embryos. Five stages of embryonic development (cleavage, blastula, gastrula, eyed stage, and heart-beating stage embryos corresponding to 7, 14, 20, 26, and 31 days post-spawning) were chosen and exposed to various cryoprotectants, namely methanol (MeOH), propylene glycol (PG), dimethyl sulfoxide (DMSO), and dimethylformamide (DMF), at three concentrations (10, 15, 20%) for an experimental period of 30 min. at culture temperature (16°C). The toxicity tolerance of E. sinensis embryos varied with developmental stage and cryoprotectant concentration. Cleavage and blastula embryos were very sensitive to cryoprotectant concentrations above 10%. Gastrula, eyed stage, and heart-beating stage embryos tolerated cryoprotectants to 20%, although a higher survival percentage was observed in heart beating
Improved fertility following artificial insemination with frozen-thawed spermatozoa would offer rabbit producers faster genetic improvement. Previous work investigating cryoprotectants for rabbit spermatozoa have reported inconsistent results. Semen was collected from three rabbit bucks by artificial vagina and frozen using a standard procedure with varied cryodiluent components. Post-thaw analysis encompassed motility, sperm kinematic parameters and acrosome and membrane integrity. Spermatozoa were evaluated at 0, 2 and 4 h after thawing. Experiment 1 compared diluents with 3.5% dimethyl sulfoxide (DMSO), 1.5% acetamide, 1.75% DMSO + 0.75% acetamide or 3.5% DMSO + 1.5% acetamide. The treatment that resulted in the highest post-thaw motility (P,0.001) and acrosome integrity (P,0.001) was DMSO alone. Experiment 2 compared 3.5, 7 and 10% DMSO in the cryodiluent. The best post-thaw sperm motility (P,0.001) and linearity (P=.002) was in 3.5% DMSO, while 10% DMSO afforded higher acrosome/membrane ...
OBJECTIVE To evaluate the effect of vitrification of mouse oocytes on the behavior of adult offspring. STUDY DESIGN Oocytes from mice were vitrified, warmed and inseminated, and two-cell embryos were transferred to foster mothers. The behavioral characterization of the offspring was detected by the Morris water maze test, forced swimming test, and elevated plus maze test, and compared to that of offspring from fresh oocytes. RESULTS Offspring produced by vitrified oocytes showed normal motor function. In the Morris water maze test of spatial learning there was a slightly decreased time spent in the quadrant containing the platform relative to mice from fresh oocytes, but the difference did not reach statistical significance. In addition, offspring from vitrified oocytes did not exhibit alterations in emotional behavior. CONCLUSION No alterations were found in the behavioral characterization of adult offspring from vitrified oocytes compared with those from fresh oocytes.
Propylene Glycol Isostearate, Propylene Glycol Laurate, Propylene Glycol Myristate, Propylene Glycol Oleate and Propylene Glycol Oleate SE are monoesters of propylene glycol and fatty acids.. SE indicates that it is a self-emulsifying form that contains some sodium and/or potassium oleate. Propylene Glycol Dicaprylate, Propylene Glycol Dicaprylate/Dicaprate, Propylene Glycol Dicocoate, Propylene Glycol Dipelargonate, Propylene Glycol Oleate, Propylene Glycol Dicaprate, Propylene Glycol Diisostearate and Propylene Glycol Dilaurate are diesters of proplyene glycol and fatty acids. The use of the "/" in the name indicates that the ingredient is a mixture of Propylene Glycol Dicaprylate and Propylene Glycol Dicaprate. In cosmetics and personal care products, Propylene Glycol monoesters and diesters are used in the formulation of moisturizers, cleansing products, fragrance products, and makeup products such as foundations and lipsticks.. ...
Sperm cryopreservation is the most efficient method for storing boar sperm samples for a long time. However, one of the inconveniences of this method is the large variation between and within boars in the cryopreservation success of their sperm. The aim of the present work was thus to find reliable and useful predictive biomarkers of the good and poor capacity to withstand the freeze-thawing process in boar ejaculates. To find these biomarkers, the amount of proteins present in the total proteome in sperm cells were compared between good freezability ejaculates (GFE) and poor freezability ejaculates (PFE) using the two-dimensional difference gel electrophoresis technique. Samples were classified as GFE and PFE using progressive motility and viability of the sperm at 30 and 240 minutes after thawing, and the proteomes from each group, before starting cryopreservation protocols, were compared. Because two proteins, acrosin binding protein (ACRBP) and triosephosphate isomerase (TPI), presented the highest
Excessive reactive oxygen species generation during sex sorting and cryopreservation of stallion sperm leads to DNA fragmentation, lipid peroxidation, and motility loss. In this study we investigated whether antioxidant supplementation during sex sorting and cryopreservation could ameliorate the effects of reactive oxygen species on stallion sperm. In experiment 1, the postthaw characteristics of stallion sperm (N = 9) cryopreserved in the presence or absence of catalase (200 U/mL), cysteine (0.2 mg/mL), or quercetin (0.15 mM) was examined. Motility and acrosome integrity were assessed at 0, 1, and 3 hours after thawing. The sperm chromatin structure assay (SCSA; detectable DNA fragmentation index [DFI], mean DFI, and DFI) was used to assess DNA integrity immediately after thawing. Quercetin increased the total postthaw motility (25.3% vs. 20.9%; P | 0.05), but there was no beneficial effect of catalase or cysteine. Based on these results, the effect of quercetin during cryopreservation on the postthaw

Polyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties.  - PubMed - NCBIPolyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties. - PubMed - NCBI

Dimethyl sulfoxide (DMSO) has been used for several decades as the most efficient cryoprotective agent (CPA) for many types of ... Polyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties.. Matsumura K1, Hyon SH. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/19515417

The influence of different freezing procedures and different cryoprotective agents on the immunological capacity of frozen...The influence of different freezing procedures and different cryoprotective agents on the immunological capacity of frozen...

Cryoprotective Agents / pharmacology*. Dimethyl Sulfoxide / pharmacology. Freezing*. Glycerol / pharmacology. Humans. Lectins ... 0/Cryoprotective Agents; 0/Lectins; 0/Mitogens; 11028-71-0/Concanavalin A; 56-81-5/Glycerol; 67-68-5/Dimethyl Sulfoxide ... The influence of different freezing procedures and different cryoprotective agents on the immunological capacity of frozen- ...
more infohttp://www.biomedsearch.com/nih/influence-different-freezing-procedures-cryoprotective/971588.html

Sulfinylbismethane
        -
        Analgesics, Non-Narcotic,  Cryoprotective Agents,  Free Radical Scavengers,  Solvents, ...Sulfinylbismethane - Analgesics, Non-Narcotic, Cryoprotective Agents, Free Radical Scavengers, Solvents, ...

Dimethyl Sulfoxide may have anti-inflammatory, antioxidant and analgesic activities. Dimethyl Sulfoxide also readily penetrates cellular membranes. The membrane-penetrating ability of dimethyl sulfoxide may enhance diffusion of other substances through the skin. For this reason, mixtures of idoxuridine and dimethyl sulfoxide have been used for topical treatment of herpes zoster in the United Kingdom ...
more infohttps://pharmacycode.com/Sulfinylbismethane.html

Intracellular pH changes in isolated bovine articular chondrocytes during the loading and removal of cryoprotective agents. -...Intracellular pH changes in isolated bovine articular chondrocytes during the loading and removal of cryoprotective agents. -...

The addition and removal of a cryoprotective agent (CPA) are necessary steps in the cryopreservation of natural or engineered ... Intracellular pH changes in isolated bovine articular chondrocytes during the loading and removal of cryoprotective agents. ... The addition and removal of a cryoprotective agent (CPA) are necessary steps in the cryopreservation of natural or engineered ... Intracellular pH changes in isolated bovine articular chondrocytes during the loading and removal of cryoprotective agents. ...
more infohttps://www.dpag.ox.ac.uk/publications/61762

Cryopreserved or Fresh Mesenchymal Stromal Cells: Only a Matter of Taste or Key to Unleash the Full Clinical Potential of MSC...Cryopreserved or Fresh Mesenchymal Stromal Cells: Only a Matter of Taste or Key to Unleash the Full Clinical Potential of MSC...

Cryoprotective agent. DMSO. Dimethyl sulfoxide. FCS. Fetal calf serum. GvHD. Graft-versus-host disease ... as a rescue agent for severe refractory acute graft-versus-host disease in pediatric patients. Biol Blood Marrow Transplant J ...
more infohttps://link.springer.com/chapter/10.1007%2F978-3-319-45457-3_7

Apitherapy News: January 2014Apitherapy News: January 2014

Evaluating with its contents, it has been shown that propolis is an appropriate cryoprotective agent in fish semen. ... Methylglyoxal-augmented manuka honey as a topical anti-Staphylococcus aureus biofilm agent: safety and efficacy in an in vivo ... The mechanism of the anti-cancer activity of honey as chemopreventive and therapeutic agent has not been completely understood ... Our results suggest that AMCH might be beneficial as a potent agent for treatment of AD-like lesion. ...
more infohttp://apitherapy.blogspot.com/2014/01/

RCSB PDB - GOL Ligand Summary PageRCSB PDB - GOL Ligand Summary Page

Cryoprotective Agents. *Cytochrome P-450 CYP2E1 Inducers. *Drugs for Constipation. *Organic Chemicals ... It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent. [PubChem]. ...
more infohttp://www.rcsb.org/ligand/GOL

Determining the Responsiveness of Intestinal Lipoprotein Production to an Elevation of Plasma Free Fatty Acids - Full Text View...Determining the Responsiveness of Intestinal Lipoprotein Production to an Elevation of Plasma Free Fatty Acids - Full Text View...

Any lipid lowering or hypoglycemic agents. *Will not donate blood three months before start or three months after completing ...
more infohttps://clinicaltrials.gov/show/NCT00152945

Idiopathic Hypertensive Anal Canal: a Place of Internal Sphincterotomy - Full Text View - ClinicalTrials.govIdiopathic Hypertensive Anal Canal: a Place of Internal Sphincterotomy - Full Text View - ClinicalTrials.gov

Cryoprotective Agents. Protective Agents. Neuromuscular Agents. Peripheral Nervous System Agents. Vasodilator Agents. ...
more infohttps://clinicaltrials.gov/ct2/show/NCT00927849?term=Hypertonia&rank=16

Global Cell and Tissue Culture Supplies Market Study: Growing Demand for Artificial Organs Noticed by Variant Market Research |...Global Cell and Tissue Culture Supplies Market Study: Growing Demand for Artificial Organs Noticed by Variant Market Research |...

Type segment includes consumable products (media, reagent, sera, contamination detection kits, cryoprotective agents) and ... Functional Clothing Fibers Protect Wearer from Chemical Agents. HemaShock Auto-Transfusion Tourniquet to Save Lives from Heart ...
more infohttps://www.medgadget.com/2017/11/global-cell-and-tissue-culture-supplies-market-study-growing-demand-for-artificial-organs-noticed-by-variant-market-research.html

The Role of Biotechnology in Exploring and Protecting Agricultural Genetic ResourcesThe Role of Biotechnology in Exploring and Protecting Agricultural Genetic Resources

6.3.4 Penetrating cryoprotective substances. Commonly used penetrating cryoprotective agents are dimethyl sulphoxide (DMSO) and ... the water content is lowered before cooling by adding high concentrations of cryoprotective agents (CPA). Thus, no ice is ... 6.3.3 Non-penetrating cryoprotective substances. Osmotic dehydration can be obtained through the application of non-penetrating ... Since plant cells rarely contain ice-nucleating agents, during a slow cooling process, crystallization is initiated first in ...
more infohttp://www.fao.org/docrep/009/a0399e/A0399E06.htm

Protective effect of intracellular ice during freezing?Protective effect of intracellular ice during freezing?

Methods to protect cells from the damaging effects of freezing have focused on the addition of cryoprotective chem ... Cryoprotective Agents*. Fibroblasts / physiology. Freezing*. Ice*. Intracellular Fluid / chemistry. Kidney / cytology, ... Methods to protect cells from the damaging effects of freezing have focused on the addition of cryoprotective chemicals and the ...
more infohttp://www.biomedsearch.com/nih/Protective-effect-intracellular-ice-during/12686211.html

MEDLINE - Results of the search |page  1|
	MEDLINE - Results of the search |page 1|

Cryoprotective Agents. Liliaceae/embryology. Meliaceae/embryology. Strychnos/embryology. Water. [Pt] Publication type:. JOURNAL ... Hypoglycemic Agents/chemistry. Hypoglycemic Agents/pharmacology. Imino Sugars/chemistry. Indolizines/chemistry. Piperidines/ ... Anti-Inflammatory Agents/isolation & purification. Anti-Inflammatory Agents/pharmacology. Female. Mice. Plant Extracts/ ... Hypoglycemic Agents/therapeutic use. Indolizines/therapeutic use. [Mh] MeSH terms secundary:. Alkaloids/pharmacology. Animals. ...
more infohttp://bases.bireme.br/cgi-bin/wxislind.exe/iah/online/?IsisScript=iah/iah.xis&base=MEDLINE&lang=i&nextAction=lnk&isisFrom=1&count=10&exprSearch=Castanospermum

Pyrogen-free excipients: offering pyrogen-free products for pharmaPyrogen-free excipients: offering pyrogen-free products for pharma

Cryoprotective agent.. In some cases, mannitol has been used for its scavenging properties. In this case, it allows to avoid ... As a physiological compatible pH regulation agent. Main characteristics of our SODIUM GLUCONATE PHARMA. *Crystalline, yellowish ... The function of dextrose as an isoosmotic isotonic agent is well established. ...
more infohttps://www.roquette.com/pharma/selected-solutions/pharma-pyrogen-free

Contemporary Techniques for Freezing Mouse Spermatozoa - Current ProtocolsContemporary Techniques for Freezing Mouse Spermatozoa - Current Protocols

Combination medium of cryoprotective agents containing l‐glutamine and methyl‐{beta}‐cyclodextrin in a preincubation medium ... This has been made possible through the use of refined cryoprotective agents and the development of improved in vitro ... Support Protocol 1: Preparation of Sperm Cryoprotective Agent Supplemented with 100 mM L‐Glutamine. ... Support Protocol 1: Preparation of Sperm Cryoprotective Agent Supplemented with 100 mM L‐Glutamine ...
more infohttp://www.currentprotocols.com/WileyCDA/CPUnit/refId-mo140065.html?quicktabs_cp=materials

Propylene glycolPropylene glycol

Pharmacological action: cryoprotective agents, solvents, vehicles.. MediLexicon propylene glycol - Medical Dictionary ...
more infohttp://scitoys.com/scichem/jqp004/1030.html

Hepatocyte - WikipediaHepatocyte - Wikipedia

"Wheat extracts as an efficient cryoprotective agent for primary cultures of rat hepatocytes". Biotechnology and Bioengineering ... in which calcium is removed to disrupt cell-cell tight junctions by the use of a calcium chelating agent. Next, a solution ...
more infohttps://en.wikipedia.org/wiki/Hepatocyte

Patent US5145769 - Method for cryopreserving blood vessels - Google PatentsPatent US5145769 - Method for cryopreserving blood vessels - Google Patents

... the type and concentration of cryoprotective agents in the media; temperature and time of exposure to those agents, cooling ... Dimethylsulfoxide was found to be more effective than other cryoprotective agents tested including glycerol, hydroxyethyl ... Owner name: GENERAL ELECTRIC CAPITAL CORPORATION, AS AGENT, MA. Free format text: SECURITY AGREEMENT;ASSIGNOR:CRYOLIFE, INC.; ... Owner name: HEALTHCARE FINANCIAL SOLUTIONS, LLC, AS AGENT, MAR. Free format text: SECURITY INTEREST;ASSIGNORS:CRYOLIFE, INC., ...
more infohttp://www.google.com/patents/US5145769?dq=6011510

Search | Journal of Biomechanical Engineering | ASME DCSearch | Journal of Biomechanical Engineering | ASME DC

Continuum Mechanics Analysis of Fracture Progression in the Vitrified Cryoprotective Agent DP6 PDF ... The Effect of Synthetic Ice Blockers on Thermal Expansion of the Cryoprotective Cocktail DP6 PDF ...
more infohttp://biomechanical.asmedigitalcollection.asme.org/solr/searchresults.aspx?q=Yoed+Rabin&p=1&s=19&c=0&t=

Fyzikální ústav AV ČR, v. v. i.Fyzikální ústav AV ČR, v. v. i.

Klíčová slova: cryoprotective agents * freezing * cryopreservation Kód oboru RIV: BO - Biofyzika Obor OECD: Biophysics. Golan, ... Freezing processes in cryoprotective solutions of ME2SO, trehalose and antifreeze protein APAFP752 and their impact on ...
more infohttps://www.lib.cas.cz/arl/publikace/riv/fzu-d

IUCr) Acta Crystallographica Section D Volume 68, Part 1, January 2012IUCr) Acta Crystallographica Section D Volume 68, Part 1, January 2012

The use of tri-methylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents ... In addition to TMAO, two other methylamine osmolytes, sarcosine and betaine, are shown to be effective cryoprotective agents ...
more infohttp://journals.iucr.org/d/issues/2012/01/00/index.html

US20130184696A1 - Cryogenic Needle with Freeze Zone Regulation 
        - Google PatentsUS20130184696A1 - Cryogenic Needle with Freeze Zone Regulation - Google Patents

Use of cryoprotective agent compounds during cryosurgery US5976505A (en) 1997-07-02. 1999-11-02. Hcs Trust. Method for ...
more infohttps://patents.google.com/patent/US20130184696A1/en
  • 3 Over the years since, more cryoprotective agents (CPAs) have been discovered. (parentsguidecordblood.org)
  • One method of storing islets intended for transplantation is via cryobanking at very low temperatures (-196 °C). Cryobanking islets without the use of cryoprotecting agents (CPAs) contributes to cellular shear stress and cell death. (pubfacts.com)
  • Background Vitrification is a method of cryopreservation by which cells and tissues can be preserved at low temperatures using cryoprotective agents (CPAs) at high concentrations (typically ⩾6.0 M) to limit the harmful effects of ice crystals that can form during cooling processes. (ualberta.ca)
  • Improved cryopreservation yield of pancreatic islets using combination of lower dose permeable cryoprotective agents. (pubfacts.com)
  • As a part of an ongoing effort to study the continuum mechanics effects associated with cryopreservation, the current report focuses on fracture formation in vitrified thin films of cryoprotective agents. (nih.gov)