Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
Substances that provide protection against the harmful effects of freezing temperatures.
The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
The transformation of a liquid to a glassy solid i.e., without the formation of crystals during the cooling process.
A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations.
Liquids transforming into solids by the removal of heat.
A method of providing future reproductive opportunities before a medical treatment with known risk of loss of fertility. Typically reproductive organs or tissues (e.g., sperm, egg, embryos and ovarian or testicular tissues) are cryopreserved for future use before the medical treatment (e.g., chemotherapy, radiation) begins.
A colorless, odorless, viscous dihydroxy alcohol. It has a sweet taste, but is poisonous if ingested. Ethylene glycol is the most important glycol commercially available and is manufactured on a large scale in the United States. It is used as an antifreeze and coolant, in hydraulic fluids, and in the manufacture of low-freezing dynamites and resins.
A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.
The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
Centers for acquiring and storing semen.
Clinical and laboratory techniques used to enhance fertility in humans and animals.
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.
Centers for acquiring, characterizing, and storing organs or tissue for future use.
The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
Procedures to obtain viable sperm from the male reproductive tract, including the TESTES, the EPIDIDYMIS, or the VAS DEFERENS.
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS.
The span of viability of a tissue or an organ.
A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.
Derivatives of propylene glycol (1,2-propanediol). They are used as humectants and solvents in pharmaceutical preparations.
The capacity to conceive or to induce conception. It may refer to either the male or female.
Cessation of ovarian function after MENARCHE but before the age of 40, without or with OVARIAN FOLLICLE depletion. It is characterized by the presence of OLIGOMENORRHEA or AMENORRHEA, elevated GONADOTROPINS, and low ESTRADIOL levels. It is a state of female HYPERGONADOTROPIC HYPOGONADISM. Etiologies include genetic defects, autoimmune processes, chemotherapy, radiation, and infections.
An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
The ratio of the number of conceptions (CONCEPTION) including LIVE BIRTH; STILLBIRTH; and fetal losses, to the mean number of females of reproductive age in a population during a set time period.
Methods pertaining to the generation of new individuals, including techniques used in selective BREEDING, cloning (CLONING, ORGANISM), and assisted reproduction (REPRODUCTIVE TECHNIQUES, ASSISTED).
Procedures to obtain viable OOCYTES from the host. Oocytes most often are collected by needle aspiration from OVARIAN FOLLICLES before OVULATION.
Inability to reproduce after a specified period of unprotected intercourse. Reproductive sterility is permanent infertility.
Damages to the EMBRYO, MAMMALIAN or the FETUS before BIRTH. Damages can be caused by any factors including biological, chemical, or physical.
An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)
The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.
The solid substance formed by the FREEZING of water.
A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.
Endometrial implantation of EMBRYO, MAMMALIAN at the BLASTOCYST stage.
Cytoplasm stored in an egg that contains nutritional reserves for the developing embryo. It is rich in polysaccharides, lipids, and proteins.
A count of SPERM in the ejaculum, expressed as number per milliliter.
Diminished or absent ability of a female to achieve conception.
A solid form of carbon dioxide used as a refrigerant.
The cap-like structure covering the anterior portion of SPERM HEAD. Acrosome, derived from LYSOSOMES, is a membrane-bound organelle that contains the required hydrolytic and proteolytic enzymes necessary for sperm penetration of the egg in FERTILIZATION.
Undifferentiated cells resulting from cleavage of a fertilized egg (ZYGOTE). Inside the intact ZONA PELLUCIDA, each cleavage yields two blastomeres of about half size of the parent cell. Up to the 8-cell stage, all of the blastomeres are totipotent. The 16-cell MORULA contains outer cells and inner cells.
The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.
The emission of SEMEN to the exterior, resulting from the contraction of muscles surrounding the male internal urogenital ducts.
A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.
Below normal weather temperatures that may lead to serious health problems. Extreme cold is a dangerous situation that can bring on health emergencies in susceptible people.
Results of conception and ensuing pregnancy, including LIVE BIRTH; STILLBIRTH; SPONTANEOUS ABORTION; INDUCED ABORTION. The outcome may follow natural or artificial insemination or any of the various ASSISTED REPRODUCTIVE TECHNIQUES, such as EMBRYO TRANSFER or FERTILIZATION IN VITRO.
The event that a FETUS is born alive with heartbeats or RESPIRATION regardless of GESTATIONAL AGE. Such liveborn is called a newborn infant (INFANT, NEWBORN).
A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.
Methods for maintaining or growing CELLS in vitro.
A plant genus of the family Gentianaceae whose members contain SECOIRIDOIDS and have been used in TRADITIONAL MEDICINE for suppressing INFLAMMATION.
The heat flow across a surface per unit area per unit time, divided by the negative of the rate of change of temperature with distance in a direction perpendicular to the surface. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.
Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION.
The inability of the male to effect FERTILIZATION of an OVUM after a specified period of unprotected intercourse. Male sterility is permanent infertility.
The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.
Solutions used to store organs and minimize tissue damage, particularly while awaiting implantation.
The process of protecting various samples of biological material.
Morphological and physiological development of EMBRYOS.
A condition of suboptimal concentration of SPERMATOZOA in the ejaculated SEMEN to ensure successful FERTILIZATION of an OVUM. In humans, oligospermia is defined as a sperm count below 20 million per milliliter semen.
Elements of limited time intervals, contributing to particular results or situations.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
Techniques for the artifical induction of ovulation, the rupture of the follicle and release of the ovum.
Transfer of preovulatory oocytes from donor to a suitable host. Oocytes are collected, fertilized in vitro, and transferred to a host that can be human or animal.
The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE.
Any of various ruminant mammals of the order Bovidae. They include numerous species in Africa and the American pronghorn.
An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.
The fertilized OVUM resulting from the fusion of a male and a female gamete.
A plant genus of the family ARECACEAE. It is a tropical palm tree that yields a large, edible hard-shelled fruit from which oil and fiber are also obtained.
The pressure required to prevent the passage of solvent through a semipermeable membrane that separates a pure solvent from a solution of the solvent and solute or that separates different concentrations of a solution. It is proportional to the osmolality of the solution.
A family of marsupials in the order Diprotodontia, native to Australia and possessing vestigial tails. There is a single living genus and species: Phascolarctos cinereus, the koala.
Morphological and physiological development of EMBRYOS or FETUSES.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
The procedure of removing TISSUES, organs, or specimens from DONORS for reuse, such as TRANSPLANTATION.
Aquaporin 3 is an aquaglyceroporin that is expressed in the KIDNEY COLLECTING DUCTS and is constitutively localized at the basolateral MEMBRANE.
The capability of bearing live young (rather than eggs) in nonmammalian species. Some species of REPTILES and FISHES exhibit this.
An absence of warmth or heat or a temperature notably below an accustomed norm.
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The techniques used to select and/or place only one embryo from FERTILIZATION IN VITRO into the uterine cavity to establish a singleton pregnancy.
A branch of embryology for the study of congenital malformations and developmental abnormalities.
Facilities that collect, store, and distribute tissues, e.g., cell lines, microorganisms, blood, sperm, milk, breast tissue, for use by others. Other uses may include transplantation and comparison of diseased tissues in the identification of cancer.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The condition of carrying two or more FETUSES simultaneously.
A complication of OVULATION INDUCTION in infertility treatment. It is graded by the severity of symptoms which include OVARY enlargement, multiple OVARIAN FOLLICLES; OVARIAN CYSTS; ASCITES; and generalized EDEMA. The full-blown syndrome may lead to RENAL FAILURE, respiratory distress, and even DEATH. Increased capillary permeability is caused by the vasoactive substances, such as VASCULAR ENDOTHELIAL GROWTH FACTORS, secreted by the overly-stimulated OVARIES.

Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay. (1/2910)

The sperm chromatin structure assay (SCSA) is a flow cytometric (FCM) technique which exploits the metachromatic properties of Acridine Orange to monitor the susceptibility of sperm chromatin DNA to in-situ acid denaturation. SCSA was used to study the chromatin structure variations of human spermatozoa in semen, both before and after swim-up and after cryopreservation. Semen samples were provided by 19 healthy normozoospermic subjects attending pre-marriage checks. Each sample was divided into three aliquots: the first aliquot was evaluated without further treatment, the second underwent swim-up, and the third was stored according to standard cryopreservation techniques in liquid nitrogen at -196 degrees C. Samples were also analysed by light and fluorescence microscopy (after Acridine Orange staining to evaluate the number of green fluorescent sperm heads), and by computer-assisted semen analysis. The results showed that post-rise spermatozoa represent a subpopulation characterized by a general improvement of the morphological (reduction of the percentage of abnormal forms and heads, increase of the green head sperm percentage) and kinetic parameters. This subpopulation also exhibited improved chromatin structure properties, confirming that these cells have the best structural and functional characteristics, indicative of optimal fertilizing ability. On the other hand, overall sperm quality deteriorates after cryopreservation. When thawed spermatozoa underwent an additional swim-up round, a general improvement of nuclear maturity was seen in the post-rise spermatozoa.  (+info)

Arterial damage induced by cryopreservation is irreversible following organ culture. (2/2910)

OBJECTIVES: The aim of the present study was to investigate the changes which occur to the arterial wall following cryopreservation and thawing and to determine whether these changes are reversible after a week of culture in an organ bath. MATERIALS AND METHODS: Rat iliac arterial segments were cryopreserved. Once thawed, the arterial segments were cultured for a period of 0, 1, 2, 4 or 7 days. Freshly isolated rat iliac vessels cultured for 7 days served as the control group. Evaluation was made of ultrastructural changes, the expression of metalloproteinase activity (MMP-1, MMP-3 and MMP-9) and the apoptotic state of cells. RESULTS: The freezing-thawing process induced damage to the arterial segments compared to fresh control vessels. After 1 week of culture, arteries showed a high degree of tissue degeneration. Only a few individual endothelial cells remained on the luminal surface. There was a gradual increase in the proportion of apoptotic cells. The sequential expression of MMP-1 during the first 2 days and subsequent expression of MMP-3 and MMP-9 were of most significance. CONCLUSIONS: Cryopreservation induced damage to the vessels which could not be reversed by organ culture. The changes observed in the expression of metalloproteinases may be indicative of the degenerative process which occurs in the extracellular matrix.  (+info)

Effect of cryopreservation on cytochrome P-450 enzyme induction in cultured rat hepatocytes. (3/2910)

In the present study, we evaluated the inducibility of cytochrome P-450 (CYP) CYP1A, CYP2B, CYP3A, and CYP4A by beta-naphthoflavone, phenobarbital, dexamethasone, and clofibric acid, respectively, in primary hepatocyte cultures prepared from both fresh and cryopreserved rat hepatocytes. Rat hepatocytes were successfully thawed and cultured after cryopreservation in liquid nitrogen for up to 1 month. Percentage of total recovery, viable cell recovery, and final viability of the cells were 68%, 72%, and 85%, respectively. Regardless of whether they were cryopreserved or not, cultured hepatocytes exhibited near-normal morphology. Treatment of cryopreserved hepatocytes with beta-naphthoflavone caused an 8-fold increase in 7-ethoxyresorufin O-dealkylase (CYP1A1/2) activity, with an EC50 of 1.5 microM; treatment with phenobarbital caused a 26-fold increase in 7-pentoxyresorufin O-dealkylase (CYP2B1/2) activity, with an EC50 of 10 microM; treatment with dexamethasone caused a 10-fold increase in testosterone 6beta-hydroxylase (CYP3A1/2) activity, with an EC50 of 1.3 microM, whereas treatment with clofibric acid caused a 3-fold increase in lauric acid 12-hydroxylase (CYP4A1-3) activity, with an EC50 of 170 microM. The induction of CYP1A, CYP2B, CYP3A, and CYP4A enzymes by these inducers was confirmed by Western immunoblotting. The patterns of P-450 induction in cryopreserved rat hepatocytes, in terms of concentration response, reproducibility, magnitude, and specificity of response, were similar to those observed in freshly isolated hepatocytes. Additionally, the magnitude and specificity of induction was similar to that observed in vivo in rats. In conclusion, under the conditions examined, cryopreserved rat hepatocytes appear to be a suitable in vitro system for evaluating xenobiotics as inducers of P-450 enzymes.  (+info)

Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. (4/2910)

When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane after different cryostorage protocols: i.e. 10% (v/v) glycerol; sperm maintenance medium (MM); freezing medium TEST yolk buffer (TYB); or cryostorage without protection (cryoshock). Using a combination of two fluorescent dyes, annexin V and propidium iodide (PI), led to three groups of spermatozoa being identified: (i) viable spermatozoa (annexin V-negative and PI-negative); (ii) dead spermatozoa (annexin V-positive and PI-positive); and (iii) cells with impaired but integer plasma membrane (annexin V-positive and PI-negative). The percentage of vital annexin V-negative spermatozoa increased significantly (P < 0.05) from spermatozoa treated by cryoshock (15.0+/-1.2%) to spermatozoa cryopreserved by TYB (26.6+/-2.2%) via cryopreservation by 10% (v/v) glycerol (19.9+/-1.6%) and by MM (22.2 1.8%) and was associated with the percentage of motile spermatozoa (17.6+/-3.4% by glycerol; 19.6+/-3.7% by MM and 22.6+/-3.9% by TYB; P = 0.0001). Of the spermatozoa, 12-22% were annexin V-positive even though they did not bind to PI, indicating viability before as well as after cryostorage. The percentage of vital annexin V-positive spermatozoa was significantly correlated with different sperm motility parameters (velocity straight linear, r = 0.601, P = 0.018; percentage of linearly motile spermatozoa: r = 0.549, P = 0.034). We, therefore, concluded that annexin V-binding is more sensitive in detecting a deterioration of membrane functions than PI staining, and that a considerable percentage of spermatozoa might have dysfunctional plasma membranes besides dead or moribund cells. Of the cryopreservation protocols tested, TYB yielded the most viable spermatozoa. Therefore, we advocate the use of the annexin V-binding assay for the evaluation of the quality and integrity of spermatozoa.  (+info)

Freezer anthropology: new uses for old blood. (5/2910)

Archived blood fractions (plasma, settled red cells, white cells) have proved to be a rich and valuable source of DNA for human genetic studies. Large numbers of such samples were collected between 1960 and the present for protein and blood group studies, many of which are languishing in freezers or have already been discarded. More are discarded each year because the usefulness of these samples is not widely understood. Data from DNA derived from 10-35-year-old blood samples have been used to address the peopling of the New World and of the Pacific. Mitochondrial DNA haplotypes from studies using this source DNA support a single wave of migration into the New World (or a single source population for the New World), and that Mongolia was the likely source of the founding population. Data from Melanesia have shown that Polynesians are recent immigrants into the Pacific and did not arise from Melanesia.  (+info)

Preparation of endometrium for egg donation. (6/2910)

Nowadays oocyte donation is a well established method of assisted reproduction and offers the unique opportunity to treat patients with various clinical indications, with or without ovarian function, in a novel way. In women with ovarian failure, artificial menstrual cycles are required before proceeding to oocyte donation. Oestrogen may be delivered in the form of oral tablets, transdermal patches in order to bypass the gastrointestinal tract thus avoiding first pass metabolism and by vaginal application. Our regimen is oestradiol valerate given in various concentrations, in order to mimic the regular cyclic fluctuations throughout the cycle. Progesterone may be administered in the form of oral tablets, intravaginal suppositories or rings and i.m. injections. Our results, as of most other groups, strongly support the vaginal route of progesterone administration. In women with retained ovarian function, synchronization of donor-recipient cycle presents a special problem, as there is strong evidence that a temporal window of maximal endometrial receptivity exists. Cryopreservation of donated embryos may be used to overcome the problem, but this approach has the important drawback of embryonic loss occurring after freezing and thawing. The method of choice is the administration of gonadotrophin-releasing hormone agonists (GnRHa) to render the patients functionally agonadal in order to circumvent cycle asynchrony between the donor and recipient.  (+info)

Turner's syndrome and pregnancies after oocyte donation. (7/2910)

A total of 20 clinical pregnancies was achieved among 18 women with Turner's syndrome who were treated in an oocyte donation programme. The oocytes were donated by voluntary unpaid donors. A mean of 1.8 embryos per transfer was given to each recipient by way of 28 fresh and 25 frozen embryo transfers. With fresh and frozen embryos, 13 and seven pregnancies respectively were achieved. The clinical pregnancy rate per fresh embryo transfer was 46%, and the implantation rate 30%, being similar to the corresponding rates among our oocyte recipients with primary ovarian failure in general. The corresponding rates with frozen embryos were 28 and 19%. Of these pregnancies, 40% ended in miscarriage. This high rate may be explained by uterine factors. Six women were hypertensive during pregnancy, a rate comparable with that in other oocyte donation pregnancies. All these women delivered by Caesarean section. Pregnancy and implantation rates after oocyte donation were high in women with Turner's syndrome, but the risk of cardiovascular and other complications is high. Careful assessment before and during follow-up of pregnancy are important. Transfer of only one embryo at a time to avoid the additional complications caused by twin pregnancy is recommended.  (+info)

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing. (8/2910)

Uncontrolled-rate freezing techniques represent an attractive alternative to controlled-rate cryopreservation procedures which are time-consuming and require high-level technical expertise. In this study, we report our experience using uncontrolled-rate cryopreservation and mechanical freezer storage at -140 degrees C. Twenty-eight PBPC samples (10 cryovials, 18 freezing bags) from 23 patients were cryopreserved in a cryoprotectant solution composed of phosphate-buffered saline (80%, v/v) supplemented with human serum albumin (10%, v/v) and dimethylsulfoxide (10%, v/v). The cryopreservation procedure required on average 1.5 h. The mean (+/- s.e.m.) storage time of cryovials and bags was 344+/-40 and 299+57 days, respectively. Although cell thawing was associated with a statistically significant reduction of the absolute number of nucleated cells (vials: 0.3x10(9) vs. 0.2x10(9), P< or =0.02; bags: 14x10(9) vs. 11x10(9), P< or =0.0003), the growth of committed progenitors was substantially unaffected by the freezing-thawing procedure, with mean recoveries of CFU-Mix, BFU-E, and CFU-GM ranging from 60+/- 29% to 134+/-15%. Mean recoveries of LTC-IC from cryovials and bags were 262+/-101% and 155+/-27% (P< or =0.2), respectively. In 14 out of 23 patients who underwent high-dose chemotherapy and PBPC reinfusion, the pre-and post-freezing absolute numbers of hematopoietic progenitors cryopreserved in bags were compared. A significant reduction was detected for CFU-Mix (11 vs. 7.4x10(5)), but no significant loss of BFU-E (180 vs. 150x10(5)), CFU-GM (400 vs. 290x10(5)) and LTC-IC (15 vs. 16x10(5)) could be demonstrated. When these patients were reinfused with uncontrolled-rate cryopreserved PBPC, the mean number of days to reach 1x10(9)/l white blood cells and 50x10(9)/l platelets were 9 and 13, respectively. In conclusion, the procedure described here is characterized by short execution time, allows a substantial recovery of primitive and committed progenitors and is associated with prompt hematopoietic recovery following myeloablative therapy even after long-term storage.  (+info)

PromoCell. *Note: Additional companies can be included on request. Moreover, customized report can be available as per the clients wishes or specific needs.. Get free exclusive sample of Cryopreservation Media market report @ The report involves an extensive study of the data available for the global Cryopreservation Media market during the historical period, 2015-2019 and makes a robust assessment of the market performance and trend for the base year, 2020. It is an in-depth analysis report of the market that offers vital insights on industry growth opportunities and development, drivers, challenges, and restrains for the global Cryopreservation Media market during the forecast period, 2021-2028.. According to analysis, the global Cryopreservation Media market was valued at USD XX million in 2019 and is projected to reach approximately USD XX million by the end of 2028, expanding at a CAGR of XX% during the forecast period ...
Embryo cryopreservation involves the generation of pre-implantation embryos using males provided by the investigator and the controlled rate cooling of collected embryos to -30°C followed by rapid freezing of embryos in liquid nitrogen to -196°C.. While embryo cryopreservation is the gold standard for preserving mouse germplasm, not all mouse strains are suitable for embryo cryopreservation. However, we have been successful at cryopreserving embryos from several different mouse strains.. Embryos for cryopreservation can be obtained in two basic ways: ...
Anderson J, Toh ZQ, Reitsma A, Do LAH, Nathanielsz J, Licciardi PV. Effect of peripheral blood mononuclear cell cryopreservation on innate and adaptive immune responses. Journal of immunological methods (2018) PubMed ...
There are many benefits of oocyte and embryo cryopreservation. When used as part of the IVF treatment process, cryopreservation can increase the likelihood of achieving pregnancy for many patients. Cryopreservation also allows couples to continue to see their families grow as IVF cycles can be repeated, years later, from the same group of eggs originally recovered for treatment.. During an IVF cycle, between 10 and 30 eggs can typically be recovered from the ovary. While it is likely that not all of these eggs will be deemed appropriate for cryopreservation, the process frequently allows couples to save a number of eggs for multiple conception attempts. In some cases, this can lead to successful treatment without the use of fertility drugs. It also reduces the likelihood that another egg retrieval procedure will be required. The number of embryos transferred depends on the number of eggs available, age, and other factors that are unique to each patient. In some cases, cryopreservation makes ...
Oocyte Cryopreservation abroad in India offers info on cost Oocyte Cryopreservation India,Oocyte Cryopreservation IVF Doctors &hospitals India.
OBJECTIVE: During IVF, non-transferred embryos are usually selected for cryopreservation on the basis of morphological criteria. This investigation evaluated an application for array comparative genomic hybridization (aCGH) in assessment of surplus embryos prior to cryopreservation. METHODS: First-time IVF patients undergoing elective single embryo transfer and having at least one extra non-transferred embryo suitable for cryopreservation were offered enrollment in the study. Patients were randomized into two groups: Patients in group A (n=55) had embryos assessed first by morphology and then by aCGH, performed on cells obtained from trophectoderm biopsy on post-fertilization day 5. Only euploid embryos were designated for cryopreservation. Patients in group B (n=48) had embryos assessed by morphology alone, with only good morphology embryos considered suitable for cryopreservation. RESULTS: Among biopsied embryos in group A (n=425), euploidy was confirmed in 226 (53.1%). After fresh single embryo
The objectives of this course are that at the conclusion of this course students should have a good understanding of: Folliculogenesis and Fertilization; Preimplantation Embryology; sperm and oocytes retrieval; criteria of selected or scoring the gametes and zygotes for cryopreservation; liquid nitrogen handling; principles of cryobiology. Students should be aware of the general aspects and implication of the cryobiology research and the potentiality that this represents for clinical application; The principles of cryobiology; The Cryoprotectants additives and how they protect the cells by stabilizing intracellular proteins; The factors that affect cellular response to freezing; The different cryopreservation protocols and what is ongoing in this field; The cross-contamination of samples in liquid nitrogen; problems in achieving a good result of cryopreservation procedure; The possible epigenetic effects of the cryopreservation procedure; testicular and ovarian tissue cryopreservation ...
Our test system specialists make each preparation with strict quality controls. Learn about our fresh and cryopreserved cell products for in vitro assays.
TY - JOUR. T1 - The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections. AU - Day, John. AU - Lorenz, Maike. AU - Wilding, Tom. AU - Friedl, Thomas. AU - Harding, Keith. AU - Pröschold, Thomas. AU - Brennan, Debra. AU - Müller, Julia. AU - Santos, Lilia. AU - Santos, Fatima. AU - Osorio, Uugo. AU - Amaral, Raquel. AU - Elster, Josef. AU - Lukavský, Jaromir. AU - Lukesova, Alena. AU - Hrouzek, P. AU - Lukes, M. AU - Probert, Ian. AU - Ryan, M.J.. AU - Benson, Erica E. PY - 2007. Y1 - 2007. N2 - Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate ...
Populations of Redside Dace Clinostomus elongatus have declined in many areas across the speciesNorth American range. Therefore, the development of sperm cryopreservation technology would provide an invaluable means of preserving genetic diversity in populations that are in imminent danger of extirpation.We developed cryopreservation protocols by testing the effects of diluent (buffered sperm motility-inhibiting saline solution [BSMIS]; BSMIS + glycine; sucrose; and Hanks balanced salt solution [HBSS]), cryoprotectant (dimethyl sulfoxide [DMSO]; propylene glycol [PG]; N,N-dimethylacetamide [DMA]; and methanol), freezing rate (1, 5, and 10◦C/min), and male-to-male variation on sperm quality. Incubating sperm in extenders affected motility;BSMIS + glycine + methanol,BSMIS + glycine + PG, and HBSS + methanol were the only treatments for which motility was not significantly different from that of fresh sperm. Sperm frozen with sucrose had higher motility than sperm frozen with BSMIS + glycine, ...
Development of effective cryopreservation protocols will be essential to realizing the potential for clinical application of neural stem and progenitor cells. Current cryopreservation protocols have been largely employed in research, which does not require as stringent consideration of viability and sterility. Therefore, these protocols involve the use of serum and protein additives, which can potentially introduce contaminants, and slow cooling with DMSO/glycerol-based cryopreservation solutions, which impairs cell survival. We investigated whether serum- and protein-free vitrification is effective for functional cryopreservation of neurosphere cultures of neural stem or progenitor cells. To protect the samples from introduction of other contaminants during handling and cryostorage, an original straw-in-straw method (250 l sterile straw placed in 500 l straw) for direct immersion into liquid nitrogen and storing the samples was also introduced. The protocol employed brief step-wise ...
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The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
TY - JOUR. T1 - Cryopreservation of human prophase I oocytes collected from unstimulated follicles. AU - Toth, T. L.. AU - Lanzendorf, S. E.. AU - Sandow, B. A.. AU - Veeck, L. L.. AU - Hassen, W. A.. AU - Hansen, K.. AU - Hodgen, G. D.. PY - 1994. Y1 - 1994. N2 - Objective: To evaluate the cryopreservation of immature human oocytes obtained from unstimulated ovarian tissue. Design: Immature prophase I oocytes were obtained from unstimulated follicles and were either cryopreserved or cultured as controls. Cryopreservation was performed in a programmable freezing machine using one of two protocols. Method I (n = 133) used a one-step addition of cryoprotectant followed by a slow freeze and thaw protocol. With method II (n = 95), the cryoprotectant was added in a stepwise manner with cryopreservation performed in the presence of 0.2 M sucrose followed by rapid freezing and thawing. Setting: Basic research center at a medical school. Patients: Patients undergoing oophorectomy for nonovarian ...
Background Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols in different species that would allow for an efficient storage of semen samples for long periods of time, ensuring its viability. Nowadays, it is widely known that an important individual component exists in the cryoresistance of semen, and efforts are aimed at identifying those sperm characteristics that may allow us to predict this cryoresistance. This knowledge would lead, ultimately, to the design of optimized freezing protocols for the sperm characteristics of each male. Methodology/Principal Findings We have evaluated the changes that occur in the sperm head dimensions throughout the cryopreservation process. We have found three different patterns of response, each of one related to a different sperm quality at thawing. We have been able to characterize males based on these patterns. For each male, its pattern remained constant among
Vitrification is an ultra‐rapid cryopreservation technique that provides excellent survivability by using optimal concentrations of cryoprotectants used in a step‐wise process supporting rapid dehydration of the oocyte. The dehydrated oocyte is loaded into a thin storage device that will facilitate ultra‐rapid cooling of the oocyte when plunged into liquid nitrogen - eliminating concerns of damaging ice‐crystal formation associated with traditional slow‐freezing procedures. Thaw solutions are formulated to support rehydration through the rapid warming process that follows vitrification to optimize cellular survival.. Irvine Scientifics vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. These solutions support implantation and pregnancy rates comparable to those of fresh cycles.. ...
Ovarian tissue cryopreservation and transplantation is a real option to preserve and restore fertility in young cancer patients. However, there is a concern regarding the possible presence of malignant cells in the ovarian tissue, which could lead to recurrence of the primary disease after reimplantation. A review of the existing literature was done to evaluate the risk of transplanting malignant cells in case of the main malignant indications for ovarian tissue cryopreservation. For ovarian tissue from patients with hematologic malignancies, it is of paramount importance to identify minimal residual disease before ovarian tissue transplantation. Indeed, these pathologies, reviewed here in detail, are considered to be most at risk of ovarian metastasis ...
The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for future use.
Global Stem Cells Cryopreservation Equipments Market: This market research report focuses on Past-Current Size, Price, Trends, Shares, Segment & Forecast
The aim of this study was to characterize the testicular isoform of angio-tensin-converting enzyme (tACE) before and after semen cryopreservation, and in the acrosome reaction of sperm from Nelore bulls in vitro. Ejaculates of 10 sexually mature Nelore bulls were used. After semen was collected, 1.0 mL of the ejaculate was used for the analysis and the rest was subjected to cryopreservation. Fresh semen before freezing, and frozen/thawed semen were centrifuged twice and the pellet was resuspended intyrodes albumin lactate pyruvate (TALP). Thereafter, 100 μL aliquots containing 100 × 106 spermatozoa were prepared. Aliquots of samples were used for western blot analysis, subjected to capacitation, and thereafter, acrosome reaction assays were performed </>in vitro</i>. With the help of an anti-ACE monoclonal antibody, a 100 kDa protein band was identified in the spermatozoa of Nelore bulls. Cryopreservation reduced the intensity of the protein bands obtained by western blot assay to less
Jenderek, M.M.; Ambruzs, B.; Tanner, J.; Holman, G.; Ledbetter, C.; Postman, J.; Ellis, D.; Leslie, C. 2014. Extending the dormant bud cryopreservation method to new tree species. In: Reed, B.M. (ed). Proceedings of the International Conference. 2. International Symposium on Plant Cryopreservation. Fort Collins (USA). 11-14 Aug 2013. Conference Paper International Society for Horticultural Science (ISHS). ISBN 978-94-62610-27-9. pp. 133-136. Acta Horticulturae. ISSN 0567-7572. no.1039 ...
CryoSearch was created by BioCision in order to help standardize the field of cryopreservation through the sharing of protocols and encouraging discussion about protocols and methods. At BioCision, our mission is to standardize sample handling. While our products help do that, products are only one part of reducing variability. The other part is how those products get used; in other words, protocols. Since we have somewhat of a specialty in cryopreservation, and there arent any other resources like CryoSearch, we thought this would be a great place to start.. Like the site? Wed love for you to check out our cryopreservation products as well. We have leak-proof and barcoded cryovials, a line of containers for slow, controlled-rate cell freezing, and tube racks that ensure precise temperature control and can also be used for snap-freezing as they are liquid-nitrogen compatible. We have a bunch of other products as well, all designed to help make your science more reliable.. Even if youre not in ...
This advisory is written for those who would like to understand the processes of mouse strain cryopreservation, why it is important and how to take advantage of the services offered. The most efficient method to protect mouse strains is by cryopreservation of gametes or embryos. Cryopreservation, and especially re-animation from frozen material, is not a trivial exercise, nor is the establishment of a cryopreservation and IVF laboratory cheap. There are numerous laboratories around the world that offer these services.
If during your egg donation procedure with fresh or frozen donor eggs more embryos were obtained than are going to be transferred into the uterus, these embryos, if of good quality, can be frozen. These frozen embryos are property of the couple or a single woman and can be used for the next attempt or a next pregnancy. They may be required if you have not got pregnant in the fresh try or if you have got pregnant and want a brother or sister for your first child several years later.. The benefit of cryopreservation is that a woman has an additional chance to conceive without new stimulation procedure and follicular puncture in the donor. Also new sperm collection is not required. Hence the transfer of frozen embryos is much less time consuming and very cost effective. It requires only one day visit for a woman and does not require a visit of a man. Preparation of uterus to the embryo transfer does not differ much from the preparation to the fresh embryo transfer.. Embryo cryopreservation is the ...
Embryo cryopreservation or embryo freezing is a method used to preserve embryos by cooling and storing them at low temperatures (-196°C). They can then be thawed at a future date and transferred to the uterus, providing additional opportunity for achieving conception.. As part of the usual process of in vitro fertilization, multiple eggs may be stimulated to grow, be recovered from the ovary and become fertilized. This may result in additional embryos in excess of the number that a couple would desire to have transferred back to the uterus at one time. If the additional embryos are of sufficiently good quality to undergo the process of cryopreservation, this can be performed in order to provide another opportunity for embryo transfer. Depending on the embryo stage at the time of freezing, between 60-90% survive freeze/thaw process resulting in a future pregnancy.. If the IVF fresh embryo transfer does not result in pregnancy, the frozen embryos can be subsequently thawed and transferred to the ...
The aim of this study was to characterize the angiotensin-converting enzyme (ACE) in Gir semen before and after cryopreservation. The ejaculate of five sexually mature bulls was used. After collection, one 1-mL aliquot of fresh semen was analyzed immediately, and the rest of the semen was cryopreserved in liquid nitrogen for subsequent analysis. Freshly collected semen and thawed cryopreserved semen were centrifuged twice with Tyrodes albumin lactate pyruvate medium (TALP) to remove plasma and extender, respectively. Samples were then subjected to western blotting, immunocytochemistry, and enzymatic activity techniques. At least one 100 kDa band was observed in every bull analyzed using western blotting with an anti-ACE monoclonal antibody, and band intensity decreased by 70% (p < 0.05) after cryopreservation. Immunocytochemistry showed periacrosomal ACE localization, and the area stained by the fluorescent antibody significantly decreased (p < 0.05) after cryopreservation. Enzyme activity was
Cryopreserved donor lymphocyte infusion (DLI) products are manufactured and administered to treat relapse after allogeneic hematopoietic stem cell transplantation. Reported clinical responses to DLIs vary broadly, even within the same group of patients. While there is an implicit recognition of the fact that different manufacturing protocols may have specific effects on different cell types, cryopreservation protocols are frequently derived from our experience in the cryopreservation of stem cell products and do not account for the heterogeneous functional nature of DLI T-cell populations. Here, we report the results of a prospective, multicenter trial on the effect of four different cryopreservation solutions that were used to freeze DLIs compared to control DLIs that were refrigerated overnight ...
Abstract: There are many reasons why cryopreservation of gametes are important: 1) maintenance of genetic diversity in domestic and wild species populations (Wildt 1992; Wildt 1997; Critser and Russell 2000), 2) facilitating the distribution of genetically superior domestic species lines, 3) treatment of human infertility (Kuczynski et al. 2001; Ranganathan et al. 2002; Tash et al. 2003; Agarwal et al. 2004; Nalesnik et al. 2004), and 4) genetic banking of genetically modified animal models of human health and disease (Critser and Russell 2000; Knight and Abbott 2002). Although cryopreservation of gametes have been routine in many other livestock industries such as the dairy industry. Cryopreservation in the swine industry is still in it infancy. Birth of live offspring has been reported from cryopreserved sperm and embryos, but success is still extremely low. From an industry perspective the low success rate has too much of an economic impact that the integration of the technology has been ...
Clinica Mar&Gen offers Testicular Biopsy Cryopreservation procedures starting from $13,400 and it is specialized in Reproductive Medicine treatments.
Taconic offers both embryo and sperm cryopreservation. Taconic also offers revitalization of cryopreserved lines. Learn more today.
An undesired side effect of cancer treatment is potential subfertility or infertility. Timely cryopreservation of semen is the best modality to ensure fertility. This retrospective data analysis established the usage rate of cryopreserved semen from cancer patients. Pubertal and post-pubertal patients who could become infertile as a result of cancer ... read more (treatment) were offered the option to cryopreserve semen prior to treatment. Of the 898 patients who cryopreserved their semen in our hospital, 96 (10.7%) used this for assisted reproductive technology. The live birth rates for intrauterine insemination, in-vitro fertilization, intracytoplasmic sperm injection and cryopreserved embryo transfer were 13%, 29%, 32% and 17%, respectively. Of all couples involved, 77% achieved parenthood, i.e. 60 of the 78 patients (with complete follow-up) fathered at least one child. show less ...
With Europe Biobank Week 2018 in Antwerp, Belgium coming up (September 4-7th) people will be reviewing the available information on the subject. A definitive book is published by Springer - and of the fourteen chapters, chapter five, The Future of Cell Preservation Strategies by John M. Baust et al will probably interest our customers.. Dr Baust introduces this subject: ... cryopreservation is often viewed as an old school discipline yet modern cryopreservation is undergoing another scientific and technology development growth phase. In this regard, todays cryopreservation processes and cryopreserved products are found at the forefront of research in the areas of discovery science, stem cell research, diagnostic development and personalised medicine. As the utilisation of cryopreserved cells continues to increase, the demands placed on the biobanking industry are increasing and evolving at an accelerated rate. No longer are samples providing for high immediate post-thaw viability ...
Excessive reactive oxygen species generation during sex sorting and cryopreservation of stallion sperm leads to DNA fragmentation, lipid peroxidation, and motility loss. In this study we investigated whether antioxidant supplementation during sex sorting and cryopreservation could ameliorate the effects of reactive oxygen species on stallion sperm. In experiment 1, the postthaw characteristics of stallion sperm (N = 9) cryopreserved in the presence or absence of catalase (200 U/mL), cysteine (0.2 mg/mL), or quercetin (0.15 mM) was examined. Motility and acrosome integrity were assessed at 0, 1, and 3 hours after thawing. The sperm chromatin structure assay (SCSA; detectable DNA fragmentation index [DFI], mean DFI, and DFI) was used to assess DNA integrity immediately after thawing. Quercetin increased the total postthaw motility (25.3% vs. 20.9%; P | 0.05), but there was no beneficial effect of catalase or cysteine. Based on these results, the effect of quercetin during cryopreservation on the postthaw
DALLAS, Texas (SEND2PRESS NEWSWIRE) -- Women delaying childbearing or requiring fertility preservation prior to undergoing treatment now have an option with the launch of oocyte cryopreservation at Fertility Specialists of Texas. Now there is an option - egg freezing - that enables women to have eggs (also known as oocytes) aspirated before undergoing cancer treatment, and frozen for later use. - News from Fertility Specialists of Texas, issued by Send2Press Newswire
Background Simple and effective cryopreservation of human oocytes would have an enormous impact on the financial and ethical constraints of human assisted reproduction. Recently, studies have demonstrated the potential for cryopreservation in an ice-free glassy state by equilibrating oocytes with high concentrations of cryoprotectants (CPAs) and rapidly cooling to liquid nitrogen temperatures. A major difficulty with this approach is that the high concentrations required for the avoidance of crystal formation (vitrification) also increase the risk of osmotic and toxic damage. We recently described a mathematical optimization approach for designing CPA equilibration procedures that avoid osmotic damage and minimize toxicity, and we presented optimized procedures for human oocytes involving continuous changes in solution composition. Methods Here we adapt and refine our previous algorithm to predict piecewise-constant changes in extracellular solution concentrations in order to make the predicted ...
course on the cryopreservation & transplantation of human ovarian tissue as well as preantral follicle isolation & in vitro culture
Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request ...
Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use.
Germinal vesicle and metaphase I oocytes should be matured in vitro before cryopreservation to optimize the reproductive potential of all retrieved oocytes.
The use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential ...
In the past two decades, laboratories around the world have produced thousands of mutant, transgenic, and wild-type zebrafish lines for biomedical research. Although slow-freezing cryopreservation of zebrafish sperm has been available for 30 years, current protocols lack standardization and yield inconsistent post-thaw fertilization rates. Cell cryopreservation cannot be improved without basic physiological knowledge, which was lacking for zebrafish sperm. The first goal was to define basic cryobiological values for wild-type zebrafish sperm and to evaluate how modern physiological methods could aid in developing improved cryopreservation protocols. Coulter counting methods measured an osmotically inactive water fraction (Vb) of 0.37+/-0.02 (SEM), an isosmotic cell volume (V(o)) of 12.1+/-0.2mum(3) (SEM), a water permeability (L(p)) in 10% dimethyl sulfoxide of 0.021+/-0.001(SEM)mum/min/atm, and a cryoprotectant permeability (P(s)) of 0.10+/-0.01 (SEM)x10(-3)cm/min. Fourier transform infrared ...
Mesenchymal stem cells (MSCs) are increasingly used as therapeutic agents as well as research tools in regenerative medicine. Development of technologies which allow storing and banking of MSC with minimal loss of cell viability, differentiation capacity, and function is required for clinical and research applications. Cryopreservation is the most effective way to preserve cells long term, but it involves potentially cytotoxic compounds and processing steps. Here, we investigate the effect of decreasing dimethyl sulfoxide (DMSO) concentrations in cryosolution by substituting with hydroxyethyl starch (HES) of different molecular weights using different freezing rates. Post-thaw viability, phenotype and osteogenic differentiation capacity of MSCs were analysed. The study confirms that, for rat MSC, cryopreservation effects need to be assessed some time after, rather than immediately after thawing. MSCs cryopreserved with HES maintain their characteristic cell surface marker expression as well as the
Attain higher post-thaw sperm recovery with the only CE marked and FDA approved medium with TEST-yolk buffer (TYB) and glycerol for freezing sperm.
I15 Should Cryoprotectants be Formulated According to the Mouse Genome?. Mike Legge, Mat Byers and Stephen Bird. Molecular Embryology Group, Department of Biochemistry, University of Otago, PO BOX 56 Dunedin, New Zealand. The use of penetrating cryporotectants such as dimethyl sulphoxide and I,2-propanediol is well established for mouse embryo cryopreservation. However, the sensitivity of both oocytes and embryos to adverse effects of both cryoprotectants and the freezing process is well documented (Duliquost et al. 1999). For mouse genome cryopreservation this is especially important as there is a correlation between mouse embryo genotype and cryopreservation success. (Schmidt et al. 1985). With the increasing necessity to archive mouse genomes by embryo and gamete cryopreservation the improvement in cryoprotectant formulations is becoming increasingly important. Recently we identified the non-enzymatic formation of formaldehyde in cryoprotectant solutions and that at the micro-molar ...
Mouse half ovaries were cryopreserved and orthotopically transplanted into ovariectomized recipients genetically identical to ovary donors except for the coat color gene. Fertility was reestablished in 57% of the female recipients, which became pregnant in an average of 40 days after transplantation of frozen-thawed half ovaries. These experiments demonstrate that ovary cryopreservation can be a very useful option for banking mouse germplasm, or managing subfertile animal colonies, when embryo or sperm freezing cannot be used or is not cost effective.
Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have darker cytoplasm than their in vivo-derived counterparts because of higher lipid accumulation. High lipid accumulation is associated with impaired embryo quality. Forskolin is an adenylate cyclase activator that regulates cAMP levels in cells and has been shown to induce lipolysis in IVP embryos. L-carnitine is required for transport of fatty acids from the intermembrane space of the mitochondria into the mitochondrial matrix to support the process of β-oxidation, and enhances ATP production. We hypothesized that the lipid content of in vivo-produced and IVP Jersey embryos is higher than respective Holstein embryos and that forskolin + L-carnitine would reduce lipid content of IVP embryos and vitrification with
It is well known that laser-assisted hatching (LAH) is the most popular and ideal embryo hatching technology, but the relevance to pregnancy outcomes of cryopreserved-thawed embryo transfer (ET) is...
Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request ...
To provide a novel fertility preservation option for patients facing a fertility threatening cancer diagnosis or treatment regimen by establishing an ovarian tissue cryopreservation program. To determine if ovarian tissue cryopreservation provides women with a useful, successful option for fertility preservation. The hypothesis is that ovarian tissue cryopreservation for fertility preservation provides an alternative option for fertility preservation. ...
BACKGROUND: Cryopreserved human sperm are used in assisted reproductive technology. However, the effect of cryopreservation on sperm DNA integrity is unclear. OBJECTIVES: The objectives of this study were to: (i) determine the impact of semen cryopreservation on human sperm DNA integrity and chromatin structure; (ii) test if parameters obtained from TUNEL and SCSA® correlate; and (iii) verify correlation between sperm motility, morphology and viability with TUNEL and SCSA® parameters. MATERIALS AND METHODS: Men attending a fertility clinic were recruited and grouped according to their sperm parameters (n = 9/group): normozoospermia, oligoasthenoteratozoospermia and teratozoospermia. Each semen sample was processed as follow: (i) directly frozen at -80 °C; (ii) diluted in Sperm Maintenance Medium, cooled for 30 min at 4 °C and frozen at -80 °C; (iii) diluted in Sperm Maintenance Medium; or (iv) in SpermFreeze. Each mixture from method (iii) and (iv) was then suspended for 30 min in liquid nitrogen
Cryopreservation is the preservation of cells, tissues or even organs at very low temperatures, with the intention of future use. At the temperature of -196oC, metabolism stops and the cells enter a state of suspended animation making it possible to store them for a long time. To achieve such a low temperature we use liquid nitrogen. The first successful cryopreservation was performed in frogs in 1945. The first animal sperm banks operated in 1949. The first successful inseminations I the human were reported in 1953 (preservation in dry ice, -70oC) and in 1964 in liquid nitrogen. Embryo cryopreservation was first performed in laboratory animals in 1972 and in human in 1984.. Today, cryopreservation is a routine method, allowing the storage of gametes and embryos for an extended period.. ...
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Whenever a sudden shift is made from fresh embryo transfer to frozen embryo transfer the couple usually have many queries in mind. So, lets evaluate both and see pros and cons.. Fresh embryos transfer is undergoing ovarian hyper stimulation making of embryos and transferring resultant embryos to uterine cavity in same cycle. In frozen embryo transfer first two steps of controlled ovarian hyper stimulation and process of IVF/ICSI are same but the resultant embryos are frozen using vitrification techniques and not transferred in same cycle. They are thawed and then kept uterine cavity after preparing the uterine cavity. In patients who are normal or hyper responders the frozen embryo transfers are known to have better results than the fresh embryo transfer . The reason can be that due to ovarian hyper stimulation and high levels of estrogen may have negative impacts on the endometrial receptivity. In some cycles another hormone called progesterone may also be raised, which again decreases embryo ...
TY - JOUR. T1 - The bioenergetics of mitochondria after cryopreservation. AU - Fuller, Barry J.. AU - Rubinacci, Alessandro. AU - Geboes, Karel. AU - De Loecker, William. PY - 1989. Y1 - 1989. N2 - The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me2SO effected some changes in mitochondrial function.. AB - The functional characteristics ...
Sometimes couples undergoing in vitro fertilization (IVF) need more than a single cycle to conceive. In recent years, frozen embryo transfers (FET) has become a popular option before moving to a fresh IVF cycle. FET at PRC allows you to extend the chance of pregnancy per egg retrieval, saving you time and money.. FROZEN EMBRYO TRANSFER (FET). A frozen embryo transfer (FET) is a cycle in which the frozen embryos from a previous fresh IVF or donor egg cycle are thawed and then transferred back into the womans uterus.. Lower Cost. The cost of frozen egg transfer is significantly lesser than a fresh cycle of IVF. This combination of reduced cost and equal success rates makes frozen embryo transfers an exciting option over fresh IVF cycle at the top IVF & egg fertility clinic in Los Angeles.. Less Medication. In a fresh cycle, you would again need to take stimulation medication. On the other hand FET IVF & egg fertility clinic in Los Angeles just requires you to use estrogen and progesterone to ...
A cohort of 91 children from cryopreserved embryos and 83 control children who were conceived normally had their development assessed using the Griffithss scales of mental development. The controls (81 singletons and two twins) of a similar age, sex, and social class were selected from siblings, cousins, and peers of the cryopreserved embryo group (68 singleton, 20 twins, and three triplets). Children from cryopreserved embryos had a lower mean birth weight and mean gestational age and a higher proportion were born by caesarean section. One child from the cryopreserved embryo group had Downs syndrome, three had squints, and four had conductive hearing loss while in the control children, six had squints, and nine had conductive hearing loss. In both groups, including the child with Downs syndrome, the mean Griffithss quotient was greater than the standard 100. In the children from cryopreserved embryos, the singleton and multiple birth subgroups had statistically similar assessment results. ...
TY - JOUR. T1 - Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation. AU - Jin, Shiying. AU - Lei, Lei. AU - Shea, Lonnie D.. AU - Zelinski, Mary B.. AU - Stouffer, Richard L.. AU - Woodruff, Teresa K.. N1 - Funding Information: Supported by Oncofertility Consortium: National Institutes of Health grants RL1-HD058295 and PL1EB008542 and Training for a New Interdisciplinary Research Workforce (T90) grants 1TL1CA133837 , U54-HD18185 , and NCRR RR00163 . PY - 2010/5/15. Y1 - 2010/5/15. N2 - Objective: To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices. Design: Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters. Setting: University-affiliated laboratory. Animals: Rhesus monkey ovarian tissue. Intervention(s): None. Main Outcome Measure(s): Histologic analysis, follicle counting, ...
BACKGROUND: Vanilla siamensis is listed in Appendix-II of Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) as an endangered species in Thailand. OBJECTIVE: To develop an optimum cryopreservation protocol for V. siamensis. MATERIALS AND METHODS: Protocorms were precultured on solid ½ MS medium with 0.5 M sucrose for 0-7 d. For encapsulation-dehydration, encapsulated protocorms (beads) were dehydrated for 0-6 h. In the case of encapsulation-vitrification, the beads were loaded with a plant vitrification solution 2 (PVS2) at 0°C for 0-90 min. RESULTS: Protocorms precultured for 3 d gave the highest post-cryopreservation survival of 17%. Dehydration of the encapsulated protocorm beads for 4 h gave the highest survival of 33% and a regrowth of 25%. Protocorms subjected to the encapsulation- vitrification method did not survive at all. CONCLUSION: Protocorms precultured with 0.5 M sucrose for 3 d, encapsulated with 3% sodium alginate and dehydrated to a ...
According to a recent report from the HFEA features a pattern towards Assisted Reproductive Technology (ART) enlistments across the globe. It states that frozen embryo transfer has been increasingly seen as a mainstream embryo transfer protocol.. HFEA measurements depict that fresh embryo transfers dropped by 11 percent somewhere in the range of 2013 to 2018. On the other hand, frozen embryo transfers nearly multiplied, representing 38% of all In Vitro Fertilization (IVF) cycles in 2018.. At one center, frozen embryo banks outperformed intercontinental donations as a treatment for donors from upcoming years after 2014. Hence, of the 1283 donor cycles finished somewhere in the range of 2005 to 2013. Out of these, 88 percent were treated as treatments from other states, yet today 95% of egg donors have moved to frozen donor eggs, in egg bank.. Also, the pattern of blastocyst transmission in some centers mirrors a similar trend now, that is currently clear in the developing enlistment information: ...
Both proceedures have seen great success rates in the last five years and thousands of baby boys and girls have been born using this technology.. Endocrine Abstracts says, Semen cryopreservation is a viable fertility preservation option for adolescent cancer patients, and should be offered to all before treatment, acknowledging the caveat of a ~50% chance of success. Pubertal staging is the only significant prognosticator of this and should be routinely assessed as part of the counselling process.. While the ethics surrounding fertility preservation in younger cancer patients are being debated, research is ongoing to take fertility tissue from patients where eggs and sperm are not necessarily matured or viable.. Speak to your health professional about fertility preservation options they may be aware of. The two of you will be able to decide if the process is right for you and decide on your next steps.. If youre wary of fertility options, remember, Geoff has managed to father two beautiful ...
Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different diatom species, representing the major morphological and ecological diatom groups, using a range of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the post-thaw recovery process. Our results show that the effects of cryoprotectant choice, its concentration and the ...
There is a lump sum due at the time of cryopreservation. This pays for the cost of transportation, cryopreservation protocols, and also for ongoing costs of maintenance for the cryonics patient. This lump sum is normally covered with a dedicated life insurance policy.. Cryonics organizations also have membership dues, which are separate from the cost of the cryonics life insurance. There are no additional costs due once a member is in cryopreservation.. ...
Cryopreservation is a reliable means for the long-term conservation of plant genetic resources. It is of particular interest for cocoa (Theobroma cacao L.) whose seeds are recalcitrant to conventional storage methods and field collections susceptible to disease infestations. However, the encapsulationdehydration procedure previously developed for cocoa somatic embryos has resulted in poor survival after retrieval from liquid nitrogen. To examine the causes of such failure, cocoa somatic embryos following each treatment step of the encapsulation-dehydration procedure were examined using a combination of confocal scanning laser microscopy and transmission electron microscopy. Results showed that the parenchymas cells of the hypocotyl and radicle were the major sites of injury possibly due to their large size and non-cytoplasmic nature, whereas the shoot meristem and provascular strand were well preserved throughout the treatments. In general, cell deformation and/or disruption was observed following the
The summer flounder, Paralichthys dentatus L., is a high-value species and considerable research has been conducted to determine practices conducive for its culture. As milt can be limited in this species, experiments were conducted to develop a practical sperm cryopreservation protocol for hatchery use. Two dilution ratios (1:2 and 1:4; sperm:extender), 2 diluents (saline and sucrose-based), 2 cryoprotectants (10% DMSO and 12% glycerol) and 3 freezing rates (?5, ?10 and ?15°C min?1) were evaluated using differential staining to assess post-thaw sperm survival. Seven combinations of the factors examined reduced post-thaw viability by less than 30%. The average viability of sperm from fresh, pooled flounder milt (67.2 ± 2.9%) was not different from that of thawed milt diluted 1:4 with sucrose diluent (10% DMSO) frozen at ?5°C min?1 (38.4 ± 7.7%) and fertilization and hatch success were not different in trials using fresh or thawed, cryopreserved sperm. From these experiments a practical
Principles of Cryopreservation and Optimization of Vitrification This talk discusses about the principles of using cryoprotectants to achieve cryopreservation including slow freezing and vitrification. Based on the principles, several approaches used to improve vitrification are reviewed. The future directions of the c
Despite of important advances in the knowledge of the reproductive biology of several deer species (mainly, red deer), there is a lot to investigate. We are trying to answer a practical and a basic question: how can we improve sperm cryopreservation in cervids? and, which underlying, cellular and molecular, changes occur during cooling and cryopreservation?. These are important questions not only for our Iberian deer species (Iberian red deer and roe deer), but also to endangered cervids elsewhere in the world (specially South-American species such as the huemul and pudu). Technology transfer from well-known species to related but less studied species might improve their conservation.. ...
TY - JOUR. T1 - Ovine semen cryopreservation usingthree extenders and four combinations of permeant and non permeantagents. AU - Rocío Sandoval, M.. AU - Alexei Santiani, A.. AU - Luis Ruiz, G.. AU - Víctor Leyva, V.. AU - Luis Coronado, S.. AU - Alfredo Delgado, C.. PY - 2007/1/1. Y1 - 2007/1/1. N2 - © 2007 Universidad Nacional Mayor de San Marcos. All rights reserved. The objective of the study was to evaluate the effect of three extenders and four combinations of two permeant and two non permeant cryoprotectant agents on the quality of post thaw ram semen. In Experiment 1, three extender were evaluated (A, B, and C) in order to select the best for the next step. In Experiment 2, different combinations of cryoprotectant agents were evaluated as follow: 1) Glycerol-Trehalose, 2) Glycerol- Sucrose, 3) Ethylene glycol-Trehalose, and 4) Ethylene glycol-Sucrose. In experiment 1, motility, viability and acrosomal integrity in extender A were higher than in extender B and extender C, and ...
IVF and ICSI are fully established methods of assisted reproduction which can be used for patients awaiting cytotoxic therapy:. Following the data of the German, Swiss and Austrian network Fertiprotekt (, 164 of 1388 counselled patients have chosen ovarian stimulation and cryopreservation of oocytes as a fertility preservation technique in 2007 2009. Among those patients 2417 oocytes were collected (Mean: 11.8, Range 0 41, STD 7.3). In 125 patients oocytes were fertilized and cryopreserved, resulting in an fertilisation rate of 70.5%/aspirated oocyte. Only in one patient, chemotherapy needed to be postponed due to severe ovarian hyperstimulation syndrome.. These data reveal that ovarian stimulation can result in adequate numbers of oocytes. However, they also demonstrate, that this technique is not successful in all patients. Combination with cryopreservation of ovarian tissue should therefore be considered. FertiPROTEKT performed a study in which 50% of one ovary was removed ...
Guidelines contents   Introduction   Recommendations   Discussing fertility   Management   Impact   Options  
Poster (2015, June 14). Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results ... [more ▼]. Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results obtained with HGL5 granulosa cell line suggest that Z-VAD-FMK is efficient to protect granulosa cells from etoposide or CoCl2 induced apoptosis. What is known already: Removal, cryopreservation and subsequent graft of ovarian strips after cancer treatment have been successfully used to re-establish female fertility. However, the pregnancy rate after autografting of cryopreserved tissue is about 30%. Indeed, the major problem after transplantation is follicular loss due to ischemic reperfusion injury. Study design, size, duration: Three human granulosa cell lines (GC1a, ...
TY - JOUR. T1 - Sucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservation. AU - Coticchio, G.. AU - De Santis, L.. AU - Rossi, G.. AU - Borini, A.. AU - Albertini, D.. AU - Scaravelli, G.. AU - Alecci, C.. AU - Bianchi, V.. AU - Nottola, S.. AU - Cecconi, S.. PY - 2006/7. Y1 - 2006/7. N2 - Background: Recently described slow-cooling cryopreservation protocols involving elevated sucrose concentration have improved survival frequencies of human oocytes, potentially overcoming a major hurdle that has limited the adoption of oocyte storage. Because implantation rates of embryos from frozen oocytes remain generally low, it is still debated whether, irrespective of survival rates, this form of cryopreservation leads inevitably to the disruption or complete loss of the metaphase II (MII) spindle. Methods: Human oocytes with an extruded polar body I (PBI) were cryopreserved using a slow-cooling method including 1.5 ...
This three-days training course is specifically designed for Embryologists interested in acquiring practical skills and updating their knowledge on oocyte, embryo, and blastocyst cryopreservation techniques. The course will focus on didactic and intensive hands-on practical lessons on the vitrification system and media that the embryologist needs to implement, pointing out the tips and tricks to achieve consistent results. Throughout the three-days, embryologists will have the opportunity to vitrify and warm unlimited number of oocytes and embryos needed to understand how important little details can affect results. The course also covers the fundamental principles of the assisted hatching procedure and its multiple applications in different developmental stages. Introduction to the Laser Assisted Hatching and familiarization with different operational modes. Evaluation and discussion about the effect of the size of the hole on the developing embryo by culturing treated embryos in a TL ...
By Leigh E. Towill (auth.), Dr. Leigh E. Towill, Prof. Dr. Y. P. S. Bajaj (eds.). Ex situ upkeep of germplasm for better plant species has been accom- plished utilizing both seeds or clones, yet garage of those less than commonplace condi- tions doesnt give you the severe longevities which are had to reduce danger of loss. expenditures of upkeep and regeneration of shares also are excessive. platforms that supply almost indefinite garage should still complement current tools and its inside this context that cryopreservation is gifted. using low temperature upkeep was once firstly extra a priority of drugs and animal breeding, and used to be multiplied to crops within the Nineteen Seventies. Sur- vival after cryogenic publicity has now been validated for various plant teams together with algae, bryophytes, fungi and better vegetation. If survival is com- monplace, then the eventual program is a cryopreservation process, wherein cells, tissues and organs are held indefinitely to be used, usually ...
The effect of cryopreservation and long-term liquid nitrogen storage on peripheral blood mononuclear cell (PBMC) subsets was prospectively analyzed using monoclonal antibodies and flow cytometry. Brief cryopreservation did not significantly alter the proportion of positively stained cells for CD3+, CD4+, CD8+, CD14+, CD16+, and CD19+ cells. A small but statistically
Research from many laboratories over the past several decades indicates that invertebrate oocytes and eggs are extraordinarily difficult to freeze. Since starfish oocytes, eggs, and embryos are an important cell and developmental biology model system, there is great interest to cryopreserve these cells. Previous starfish oocyte cryopreservation studies using slow cooling protocols revealed that these cells are highly sensitive to osmotic stress and form intracellular ice at very high sub-zero temperatures, suggesting that common freezing methodologies may not prove useful. We report here that a short exposure to 1.5 M Me2SO/1 M trehalose in hypotonic salt solution followed by ultra-rapid cooling to cryogenic temperatures allows starfish oocytes to be cryopreserved with the average survival rate of 34% when normalized to control oocytes that were exposed to CPA, but not frozen. On average, 51% of the oocytes in 77% of the batches of frozen oocytes underwent meiotic maturation in response to the ...
Since the discovery of Vitrification in 2008, the pregnancy rate of frozen embryos is exactly the same or, in some cases, higher than that of fresh embryos transferred after In Vitro Fertilisation. Pregnancy rates are five times higher than the gestation rates of intrauterine insemination, which is why frozen embryo transfer is currently one of the most effective and safest techniques to welcome a baby home.. Frozen embryo transfer is the most effective and safest technique if we want to achieve a full-term pregnancy in cases of infertility or desire to be a mother. Pregnancy rates are related to the age of the couple, especially the mother, since it clearly accentuates the possibility of gestation.. ...
Egg freezing and cryopreservation services provided by Dr. Akin at Bluegrass Fertility Center located in Lexington, KY. Other services include IVF, Assisted Hatching, ICSI and PGD.
Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to Show moreCryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to assess the ...
The delivery rate achieved after the vitrification of early cleavage- and blastocyst-stage embryos is not affected by the embryo developmental stage or any other variable related to the warming cycle.
Frozen embryo transfers have higher success rates than fresh embryo transfers. Within frozen embryo transfers there are natural and programmed cycles. Each have tradeoffs in terms of cost, intensity, drug requirements and more.
Results of this study10 were surprising, to say the least. Without going into the specifics of different types of fertility preservation and their respective indications, less than half of the respondents (45.6%) reported being aware of fertility preservation. Specialists in O&G fared no better in this regard with only half (50.7%) of the respondents reporting themselves as being aware. As expected, O&G specialists were more aware of fertility-preservation techniques in females such as oocyte- and embryo-freezing as well as ovarian tissue freezing, than their non-O&G counterparts. Interestingly, when respondents were further asked about individual fertility-preservation procedures, an increased awareness was found. In fact, a higher percentage of the same O&G specialists in this study reported to be familiar with all of the above fertility-preservation techniques previously itemised, compared with being aware of fertility preservation per se (63.6% vs 50.7%). These findings highlight a ...
Ovarian tissue cryopreservation (OTC) is offered to women treated for acute leukemia to preserve their fertility before hematopoietic stem cell transplantation. The risk of leukemic infiltration in ovarian samples harvested before administration of chemotherapy limits ovarian tissue transplantations. We assessed the minimal residual disease (MRD) by sensitive quantitative polymerase chain reaction in cryopreserved ovarian cortex and medulla samples harvested from 30 patients in complete remission of acute leukemia, including 60 % with negative bone marrow MRD at the time of OTC. Ovarian MRD was undetectable in 21 patients (70%), detectable below 10-4 in 8 patients (27%) and between 10-3 and 10-4 in 1 patient (3%). Twenty patients (67%) had concordant MRD between bone marrow and ovarian samples. Interestingly 4 patients had positive MRD in ovarian samples while undetectable in bone marrow. Our results underline the importance of reaching the best control of the disease with undetectable or low ...
Mary Zelinski, PhD finishes her reports from the annual meeting of the Society for Cryobiology held from Corvallis, Oregon with a final blog about the keynote
Knowing that there are options for many women to protect or preserve their reproductive potential before cancer treatment begins can make it less challenging to discuss the possible reproductive side effects of treatment with your patients
TY - JOUR. T1 - Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. AU - Teo, Ka Yaw. AU - DeHoyos, Tenok O.. AU - Dutton, J. Craig. AU - Grinnell, Frederick. AU - Han, Bumsoo. N1 - Funding Information: This research was supported by grants from the National Institute of Health / National Institute of Biomedical Imaging and Bioengineering , R01 EB008388 , and the National Science Foundation , CBET-1009465 . PY - 2011/8. Y1 - 2011/8. N2 - The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In ...
Not all frozen embryo transfer cycles and protocols are the same; however, I am sharing my FET cycle to provide insight into how FET cycles can go.
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The purpose of this study was to test of efficacy of melatonin on the Optimizing of cryopreservation media in the testis tissue samples. Testes from neonate BALB/c mice were vitrified and then thawed under standard condition with or without the addition of 100 μM melatonin to both of vitrification and thawing solution. After that, Vitrified-thawed whole testes were digested under standard condition and subsequent viability of the cells in the suspension was analyzed using cytotoxicity kit and Apo-Brdu tunnel assay kit. The mean proportion of apoptotic testicular cells in the treated vitrified-thawed testes in comparison to no-treated ones was noted significantly (5.2±0.47 vs. 1.56±0.62, respectively). Moreover, melatonin cause decreasing the viability of the treated vitrified-thawed testicular cells in compared to no-treated vitrified-thawed testicular cells (4.78±0.46 vs. 8.39±0.76, respectively). In addition, the mean cytotoxicity of melatonin on the vitrified-thawed testicular cells was ...
What are benefits of frozen embryo transfer? Before the advent of embryo freezing , surplus embryos had to be discarded after fresh embryo transfer. Read on to find out more
PubMed journal article: Successful elective and medically indicated oocyte vitrification and warming for autologous in vitro fertilization, with predicted birth probabilities for fertility preservation according to number of cryopreserved oocytes and age at retrieval. Download Prime PubMed App to iPhone, iPad, or Android
FERRARI, Edilene Aparecida Preti; COLOMBO, Ronan Carlos; FARIA, Ricardo Tadeu de and TAKANE, Roberto Jun. Criopreservação de sementes de Encholirium spectabile Martius ex Schultes f. pelo método da vitrificação. Rev. Ciênc. Agron. [online]. 2016, vol.47, n.1, pp.172-177. ISSN 0045-6888. A bromélia Encholirium spectabile Martius ex Schultes f. é uma espécie endêmica da Caatinga. O objetivo deste trabalho foi avaliar a eficiência de soluções crioprotetoras em sementes de bromélia. Os tratamentos consistiram da imersão das sementes em soluções crioprotetoras e de vitrificação, antes da imersão em nitrogênio líquido (NL) (-196 ºC), conforme os tratamentos a seguir: T1 - controle: sem crioprotetores; T2 - glicerol 2M (20 min) + PVS2 (10 min); T3 - glicerol 2M (20 min) + PVS2 com floroglucinol a 1% (10 min); T4 - sacarose 0,4M (20 min) + PVS2 (10 min); T5 - sacarose 0,4M (20 min) + PVS2 com floroglucinol a 1% (10 min); T6 - glicerol ...
The Fertility Preservation Program also will endeavor to aid women and girls who are starting cancer treatment or preparing for a bone marrow transplant. Currently their choices are quite limited and, for many, biologically impossible or morally unacceptable, noted Joseph S. Sanfilippo, M.D., director of the Center for Fertility and Reproductive Endocrinology at Magee-Womens Hospital.. Women can undergo ovarian stimulation to generate multiple mature eggs, which can then be fertilized with partner or donor sperm to produce embryos for later implantation and possible pregnancy, he said. These techniques are now well-established, and we will offer them to female patients who wish to pursue this option. Another option is ovarian cryopreservation, where an ovary is removed before cancer therapy is initiated with the plan to replace ovarian tissue once the patient is cured.. Still, the stimulation technique takes time and could delay cancer treatment. It also produces high estrogen levels that ...
Perry SF (1995). "Freeze-drying and cryopreservation of bacteria". Cryopreservation and Freeze-Drying Protocols. Methods in ... in embryo cryopreservation Ovarian tissue in ovarian tissue cryopreservation Plant seeds or shoots tips or dormant buds are ... "Principles of cryopreservation". Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology. Vol. 368. pp. 39- ... Cryopreservation for embryos is used for embryo storage, e.g., when IVF has resulted in more embryos than is currently needed. ...
Panis B, Swennen R (1995). "Cryopreservation of Germplasm of Banana and Plantain (Musa Species)". Cryopreservation of Plant ... Aside from the challenges involved with cryopreservation in general, an important hurdle, when developing cryopreservation ... While this cryopreservation conservation strategy can be used on all plants, it is often only used under certain circumstances ... Plant cryopreservation is a genetic resource conservation strategy that allows plant material, such as seeds, pollen, shoot ...
The cryopreservation of embryos was first successfully attempted in 1984 in the case of Zoe Leyland, the first baby to be born ... Embryo cryopreservation is useful for leftover embryos after a cycle of in vitro fertilisation, as patients who fail to ... The practice of cryopreservation of embryos has increased in recent years. While the original purpose of freezing embryos was ... Cryopreservation enables the embryos to be safely stored for extensive periods of time. Individuals are then able choose the ...
Cryopreservation itself has always played a central role in assisted reproductive technology. With the first cryopreservation ... Human oocyte cryopreservation (egg freezing) is a procedure to preserve a woman's eggs (oocytes). This technique has been used ... Oocyte cryopreservation can increase the chance of a future pregnancy for three key groups of women: those diagnosed with ... Oocyte cryopreservation is an option for individuals undergoing IVF who object, either for religious or ethical reasons, to the ...
Cryopreservation of animal genetic resources § Semen Frozen bovine semen Oocyte cryopreservation Reed, ML; Ezeh, PC; Hamic, A; ... Semen cryopreservation (commonly called sperm banking or sperm freezing) is a procedure to preserve sperm cells. Semen can be ... Di Santo, M; Tarozzi, N; Nadalini, M; Borini, A (2012). "Human Sperm Cryopreservation: Update on Techniques, Effect on DNA ... Kopeika, J.; Thornhill, A.; Khalaf, Y. (2014). "The effect of cryopreservation on the genome of gametes and embryos: principles ...
... is cryopreservation of tissue of the ovary of a female. Cryopreservation of ovarian tissue is ... Oktay K, Oktem O (November 2008). "Ovarian cryopreservation and transplantation for fertility preservation for medical ... Live birth after ovarian tissue autotransplantation following overnight transportation before cryopreservation. Fertil Steril. ...
In the future, cryopreservation of testicular tissue has the potential to be used to help transgender women have children. ... Cryopreservation can either be done by slow freezing or vitrification. Slow freezing allows the temperature of the cells and ... Cryopreservation of testicular tissue is an experimental method being used to preserve fertility in pre-pubescent males, or ... Instead, cryopreservation of testicular tissue prior to cancer treatment can be offered to preserve fertility. This is ...
"Cryopreservation". Archived from the original on 15 November 1999. "HFEA - Storage of Gametes and Embryos". Archived from the ... Sperm, eggs and embryos are stored in liquid nitrogen using cryopreservation (defined as the freezing of cells or whole tissues ... Includes safety procedure regulations at fertility clinics; includes safe cryopreservation of eggs and embryos. Eggs and ... A cryoprotective compound (a liquid called cryopreservation medium), along with carefully controlled cooling and warming cycles ...
Pai, H. D.; Baid, R.; Palshetkar, N. P.; Pai, A.; Pai, R. D.; Palshetkar, R. (2021). "Oocyte Cryopreservation". Journal of ... "Oocyte Cryopreservation - Current Scenario and Future Perspectives: A Narrative Review", 2021, Hrishikesh D Pai,1 Rashmi Baid,1 ...
Chian, Ri-Cheng (2010). Fertility Cryopreservation. Cambridge University Press. p. 52. ISBN 9780521517782. v t e (Male genital ...
Cryopreservation was applied to human cells beginning in 1954 with frozen sperm, which was thawed and used to inseminate three ... Cryopreservation has long been used by medical laboratories to maintain animal cells, human embryos, and even some organized ... Cryopreservation may be accomplished by freezing, freezing with cryoprotectant to reduce ice damage, or by vitrification to ... In London in 2016, the English High Court ruled in favor of a mother's right to seek cryopreservation of her terminally ill 14- ...
He was also Head of the Tissue Cryopreservation Section of the Transfusion and Cryopreservation Research Program of the U.S. ... He received the Cryopreservation Award from the International Longevity and Cryopreservation Summit held in Madrid, Spain in ... Fahy GM, Wowk B, Wu J, Phan J, Rasch C, Chang A, Zendejas E (2004). "Cryopreservation of organs by vitrification: perspectives ... As a scientist with the American Red Cross, Fahy was the originator of the first practical method of cryopreservation by ...
Pegg, DE (2007). "Principles of Cryopreservation". In Day JG, Stacey GN (eds.). Cryopreservation and Freeze-Drying Protocols. ... Cryopreservation and Freeze-Drying Protocols, Second Edition. Methods in Molecular Biology. Vol. 368. Humana Press. pp. 39-57. ...
ISBN 9783662035337., In Kubitzki & Huber (1998). Panis, Bart (2008). "Cryopreservation of monocots". Plant Cryopreservation: A ... 2008). Plant cryopreservation a practical guide. New York: Springer. ISBN 978-0-387-72276-4. Short, Emma; George, Alex (2013). ...
Abazari A, Jomha NM, Elliott JA, McGann LE (2013). "Cryopreservation of articular cartilage". Cryobiology. 66 (3): 201-209. doi ...
Chen, C (April 1986). "Pregnancy after human oocyte cryopreservation". Lancet. 1 (8486): 884-6. doi:10.1016/S0140-6736(86)90989 ... Gook, Debra A. (1 September 2011). "History of oocyte cryopreservation". Reproductive BioMedicine Online. 23 (3): 281-289. doi: ...
Cryopreservation of embryos is dependent on the species and the stage of development of the embryo. Pig embryos are the most ... Cryopreservation requires equipment to collect biological material and test tubes for storage. Price is highly variable based ... "Cryopreservation of the Germplasm of Animals Used in Biological and Medical Research: Importance, Impact, Status, and Future ... "Cryopreservation of Human Embryos by Vitrification or Slow Freezing: A Systematic Review and Meta-analysis." Techniques and ...
It is also commonly used in cryopreservation of blood vessels along with the other glycosaminoglycans and protein suspensions. ... Müller-Schweinitzer E, Hasse J, Swoboda L (1993). "Cryopreservation of human bronchi". J Asthma. 30 (6): 451-7. doi:10.3109/ ... Müller-Schweinitzer E, Ellis P (May 1992). "Sucrose promotes the functional activity of blood vessels after cryopreservation in ... Brockbank KG (February 1994). "Effects of cryopreservation upon vein function in vivo". Cryobiology. 31 (1): 71-81. doi:10.1006 ...
Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...
Some of the company founders had past experience in the field of cryopreservation. For instance, in 2003, Igor ARyukhov was the ... Almost as many as 200 Russian citizens have entered into cryopreservation agreements with the company. KrioRus is considered to ... José Luis Cordeiro (2016-03-09). "Immortality and Plan B: Cryopreservation". Archived from the original on ... Yuri Pichugin; Gregory M. Fahy; Robert Morin (April 2008). "Cryopreservation of rat hippocampal slices by vitrification". ...
Henkelman S, Lagerberg JW, Graaff R, Rakhorst G, Van Oeveren W (November 2010). "The effects of cryopreservation on red blood ... These techniques can be used to assess the success of cell culture techniques, cryopreservation techniques, the toxicity of ... Pichugin Y, Fahy GM, Morin R (April 2006). "Cryopreservation of rat hippocampal slices by vitrification". Cryobiology. 52 (2): ... Crutchfield A, Diller K, Brand J (1999-02-01). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal ...
Cryopreservation Freeze-drying Pilhofer, Martin; Ladinsky, Mark S.; McDowall, Alasdair W.; Jensen, Grant J. (2010). "Bacterial ...
He did important translational research on ovarian tissue cryopreservation and transplantation. Gosden was born on 23 September ... Prasath, Ethiraj B. (1 January 2008). "Ovarian tissue cryopreservation: An update". Journal of Human Reproductive Sciences. 1 ( ... Silber, S. J. (1 February 2012). "Ovary cryopreservation and transplantation for fertility preservation". Molecular Human ... and he did important translational research on ovarian tissue cryopreservation and on ovary transplantation; his interests also ...
"Cryopreservation of Mammalian Cells - US". Retrieved 2022-12-02. Freed, Lisa E.; Guilak, Farshid (2007-01 ...
Donor blood cryopreservation also reduces the number of times a donor needs to have blood drawn for cross matching, making ... The implementation of the cryopreservation service allowed NKR centers to dramatically reduce the time to complete a cross ... "National Kidney Registry Initiates Donor Blood Cryo-Preservation" (Press release). National Kidney Registry. December 2, 2014. ... Berz; McCormack; Winer; Colvin; Quesenberry (November 12, 2007). "Cryopreservation of Hematopoietic Stem Cells". Am. J. Hematol ...
Porcu, Eleonora; Ciotti, Patrizia; Venturoli, Stefano (2012-12-06). Handbook of Human Oocyte Cryopreservation. Cambridge ... useful for applications such as sperm cryopreservation. The semi-solid mixture can also be called slush nitrogen or SN2. Solid ... in liquid and slush nitrogen by numerical simulation of cooling rates for French straws used for sperm cryopreservation". ...
2005) Biology of Plants, 7th ed., page 459 Reed, Barbara (2008). Plant cryopreservation a practical guide. New York: Springer. ...
The use of cryopreservation agents is also key to the freezing process. A common cryoprotection agent used is 10% solution of ... Coopman, K (2013). Cryopreservation: Technologies, Applications and Risks/Outcomes (PDF). Nova Science Publishers. pp. 91-108. ... Rapid thaws are recommend in bringing the cells out of cryopreservation and starting up their normal metabolic processes. ... Cell banks are commonly used within fields including stem cell research and pharmaceuticals, with cryopreservation being the ...
These methods include: Cryopreservation Mummification; the most well-known examples are from ancient Egypt Taxidermy; an ...
In 1983, Alan Trounson and Linda Mohr reported the first pregnancy which used embryo cryopreservation (frozen human embryos). ... Christopher Chen reported the first pregnancy which used oocyte cryopreservation (frozen eggs). The ability to freeze sperm has ... Chen, Christopher (1986-04-19). "Pregnancy After Human Oocyte Cryopreservation". The Lancet. Originally published as Volume 1, ...
Read this new article to learn about the successes of cryopreservation. What are the latest techniques and how do they work? ... Cryopreservation of oocytes and embryos is a fashionable subject of reviews. This is not only the subjective impression of the ... During the past decade, cryopreservation of oocytes and embryos has become one of the most exciting areas in human-assisted ... is dealing with embryo and oocyte cryopreservation. The number is disproportionally low, especially if it is compared with the ...
Please contact Leo Ennen ([email protected]) to learn more about cryopreservation, revitalization and sanitation at the NKI. ... In close collaboration with the NKIs Animal Facility, we perform cryopreservation of mouse strains by freezing sperm or embryos ...
Cryopreservation of the brain could be used for cryogenic suspension of those who are critically ill for possible revival in ... Abstract - Aldehyde-stabilized cryopreservation. We describe here a new cryobiological and neurobiological technique, aldehyde- ... stabilized cryopreservation (ASC), which demonstrates the relevance and utility of advanced cryopreservation science for the ... Rabbit brain defrosted from cryopreservation without damage. April 7, 2017. February 12, 2016. by Brian Wang ...
Cryopreservation, Breeding and Maintenance of genetically altered mice as a service ... Cryopreservation, Breeding and Maintenance of genetically altered mice as a service This service licence allows the University ... Read cryopreservation, breeding and maintenance of genetically altered mice as a service non-technical summary (PDF). This PDF ... altered rodent colonies and the preservation and archiving of important genetically altered rodent lines by cryopreservation to ...
The findings of the present study indicated that cryopreservation of sperm in glucose-Tris-based extender using 0.5-mL straws ... 0.5 mL) on Nile tilapia sperm quality after cryopreservation. Sperm was frozen according to conventional slow freezing ... Cryopreservation in Eukaryotes Edited by Francisco Marco-Jimenez. Cryopreservation in Eukaryotes. Edited by Francisco Marco- ... During the cryopreservation process, some factors may change the physiological status of sperm. The success of cryopreservation ...
Cryopreservation. Cryopreservation or cell freezing is the process of using low temperatures to preserve cells and tissues for ... To learn about the fundamentals of cryopreservation, view our Cryopreservation Basics Guide > ... Tech Tip Cryopreservation and Thawing of Pluripotent Stem Cells: Technical Tips and Media Recommendations ...
Ovarian Tissue Cryopreservation and Transportation. Society for Low Temperature Biology (SLBT). 26 May 2020. ... In collaboration with I³, SLTB will organize a session on ovarian tissue cryopreservation. ...
Robust cryopreservation solutions optimized for enhanced performance across many cells, including stem and T cells. Maintain ... NutriFreez® cell freezing solutions are designed and validated for the cryopreservation of cells including stem cells, T cells ... They support animal component-free conditions during cryopreservation, essential to maintaining consistency when culturing ...
... Multiple formulation available to help cell recovery, attachment and growth. ...
CryoMACS® Freezing Bags are individually packed and highly durable for safe cryopreservation. , Suomi ... Bags for safe cell culture and cryopreservation CryoMACS® Freezing Bags and CryoMACS DMSO are the right step towards safe ... cryopreservation of cells at ultra-low temperatures. Miltenyi Biotec offers MACS® GMP Cell Differentiation and MACS GMP Cell ...
... is cryopreservation and egg freezing the same thing with regard to infertility treatment? ... Dr Silber answers questions on FB Live - is cryopreservation and egg freezing the same thing with regard to infertility ...
Cryopreservation was efficient for the embryonic axes desiccated to 13%-15% moisture content. Freezing of embryonic axes at ... Successful cryopreservation was achieved with all three methods using embryonic axes; however, the recovery growth percentage ... after cryopreservation. Out of the three methods employed, the encapsulation-dehydration method gave the best recovery values ... after cryopreservation. In the encapsulation-dehydration method, encapsulated axes precultured on 0.5 M sucrose medium followed ...
Cryopreservation Barrel-Aged Pistachio Ice Cream Stout: Anaheim, CA ... Cryopreservation Barrel-Aged Pistachio Ice Cream Stout. 500 ml Bottle. $25.99 ...
Animals, Cell Membrane, Cell Survival, Cryopreservation, Fertility, Freezing, Male, Mitochondria, Sea Bream, Semen Preservation ... Cryopreservation produces several types of damage in spermatozoa, leading to fertility impairment. The reduction arises both ... In the present study, we have analysed the effect of cryopreservation in 5 ml macrotubes on the quality of post-thawed gilthead ... Mitochondrial status was affected by cryopreservation since there was a decrease in the parameters of sperm motility, ATP ...
... zeteki spermatozoa after cryopreservation can be achieved by equilibrating the ARS-diluted samples for 5 min at 4 °C in CPA3- ... IMPACT OF MEDIUM OSMOLALITY AND CRYOPRESERVATION ON MOTILITY AND CELL VIABILITY. ...
Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo. Stem Cell ... Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo. In: Stem ... Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo. / Wakeman ... title = "Cryopreservation Maintains Functionality of Human iPSC Dopamine Neurons and Rescues Parkinsonian Phenotypes In Vivo", ...
3 comment on "Elective fertility cryo-preservation instigates debate in the Netherlands" * perceval. ... For a few years, technology has been available for the cryo-preservation of oocytes or ovarian tissue, which is used to help ... We argue that there are no convincing a priori moral reasons why cryopreservation of ovarian tissue or oocytes should not also ... Elective fertility cryo-preservation instigates debate in the Netherlands. November 30, 2022Rense Nieuwenhuis ...
Ex situ conservation and cryopreservation of orchid germplasm. David Merritt, F. Hay, N.D. Swarts, K.D. Sommerville, Kingsley ... Dive into the research topics of Ex situ conservation and cryopreservation of orchid germplasm. Together they form a unique ...
2018 15 years have passed since the first cryopreservation procedure was conducted outside the United States. The first patient ... 15 years anniversary of a cryopreservation. May 12, 2018 15 years have passed since the first cryopreservation procedure was ... a CryoGen ICO was started issuing cryptocurrency CRYO tokens that will become the official means of paying for cryopreservation ...
Tämä sähköpostiosoite on suojattu spamboteilta. Tarvitset JavaScript-tuen nähdäksesi sen.. Henkilökohtaiset sähköpostiosoitteet: ...
Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm Olvido Chereguini1, Rosa Maria Cal2, Catherine ... Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two ... Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl ... Cryopreservation of great scallop (Pecten maximus) sperm: effect of extender, cryoprotectant and cooling rate on sperm survival ...
A discussion on the integration of high cell density cryopreservation in the seed train andkey considerations for developing a ... In this white paper we discuss integration of high cell density cryopreservation (HCDC) in the seed train and explore key ... considerations for developing a cryopreservation process including choice of cryoprotectant and freezing techniques. ...
... ... FUJIFILM Irvine Scientifics PRIME-XV Stem FreezIS DMSO-Free* cryopreservation medium is a complete and ready-to-use, ... The medium is validated for use in mesenchymal stromal cells and CD34+ cells, and optimized cryopreservation protocols for ... For more information on FUJIFILM Irvine Scientifics PRIME-XV Stem FreezIS DMSO-Free* cryopreservation medium, please visit ...
Ovarian tissue cryopreservation and transplantation is a promising option for fertility preservation in pre-pubertal girls and ... Ovarian tissue cryopreservation and transplantation is a promising option for fertility preservation in pre-pubertal girls and ... Ovarian tissue cryopreservation and transplantation is a promising option for fertility preservation in pre-pubertal girls and ... Ovarian tissue cryopreservation and transplantation is a promising option for fertility preservation in pre-pubertal girls and ...
View details for Effect of cryoprotectants exposure and cryopreservation on zebrafish (Danio rerio) ovarian tissues. ... Effect of cryoprotectants exposure and cryopreservation on zebrafish (Danio rerio) ovarian tissues. Authors: Zhang, T., ... Conference: Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissues and Organs. ...
Cryopreservation and re-establishment of neurospheres:. Step 8 - add the contents of the cryovial slowly to the conical tube ... Cryopreservation and re-establishment of neurospheres:. *. Pool the culture medium with the neurospheres from a 24 multi-well ... Cryopreservation and re-establishment of neurospheres:. If low numbers of neurospheres are obtained after the freezing process ... Procedures for the long term passage of neurospheres and the cryopreservation of neurospheres are also provided. In addition to ...
... Embryo freezing involves the freezing and storing of embryos obtained by ovarian stimulation, egg ...
  • The CARD-RPCI Sperm and Embryo Cryopreservation Workshop was recently held in Buffalo NY USA on the campus of Roswell Park Cancer Institute. (
  • Lluis Montoliu provided lectures in current methods of embryo cryopreservation and CRISPR/Cas9 methods of generating genetically engineered mice, and Barbara Stone provided training in using the NSET method of embryo implant. (
  • Mature oocyte and embryo cryopreservation are established fertility preservation methods associated with high success rates. (
  • Embryo cryopreservation is a well-established technique and has been used in fertility centers worldwide for the past 30 years in noncancer patients. (
  • During the past decade, cryopreservation of oocytes and embryos has become one of the most exciting areas in human-assisted reproduction. (
  • Cryopreservation of oocytes and embryos is a fashionable subject of reviews. (
  • In close collaboration with the NKIs Animal Facility, we perform cryopreservation of mouse strains by freezing sperm or embryos to conserve new strains and strains not needed in the near future to reduce the amount of alive animals in stock. (
  • The remaining embryos can be preserved in the lab, via a method known as cryopreservation, so as to remain available for future use if that is deemed desired. (
  • Improved cryopreservation of embryos in the field of IVF would increase fertility odds for Would-Be parents and the health of their future babies. (
  • IMSEAR at SEARO: Cryopreservation of mouse embryos at -196 degrees C by vitrification. (
  • Agrawal KP, Polge C. Cryopreservation of mouse embryos at -196 degrees C by vitrification. (
  • What is the Process of Cryopreservation of Embryos? (
  • 2) suggested that cryopreservation of bilateral ovarian cortex followed by COH is a feasible and safe approach to preserve fertility before gonadotoxic treatment, and that the number of cryopreserved embryos was similar to the controls. (
  • This is not only the subjective impression of the authors, it can be justified by a simple PubMed search for 'cryopreservation' or 'vitrification' or 'freezing', and 'oocyte' or 'embryo', with the additional filters for '10 years', 'humans' and 'review', resulting in 360 hits, with 87 of them focusing primarily on cryopreservation (based on individual evaluation of abstracts performed by authors). (
  • One of our arguments is that out of the 486 thematic papers published in the seven volumes of Expert Review of Obstetrics and Gynecology , only three (including a short Editorial) is dealing with embryo and oocyte cryopreservation. (
  • However, established methods for fertility preservation, including embryo or oocyte cryopreservation, are not always suitable for female cancer patients because of complicated individual conditions and treatment methods. (
  • Oocyte cryopreservation is a tool that allows you to keep the probabilities of becoming a mother with your own eggs, independently from your biological age. (
  • Oocyte cryopreservation is also an alternative for patients who have to undergo an oncological treatment, ovarian surgery, endometriosis or that suffer from any illness that damages their fertility. (
  • Oocyte cryopreservation and storage of frozen eggs during the first year. (
  • Controlled ovarian stimulation (COS) followed by embryo and/or oocyte cryopreservation is the FP method recommended by both the ASCO and ISFP. (
  • Currently, oocyte cryopreservation seems to be the most feasible technique for fertility preservation when there's some kind of a time constraint in adolescents and adults. (
  • Predicting the likelihood of live birth for elective oocyte cryopreservation: a counseling tool for physicians and patients. (
  • The study of the seed storage behavior and cryopreservation of embryonic axes was attempted in Citrus jambhiri genotypes using air desiccation-freezing, vitrification, and encapsulation-dehydration methods. (
  • In the vitrification method, embryonic axes subjected to PVS2 treatment for 40 min had the highest recovery rate of 25% after cryopreservation. (
  • Drs. Naomi Nakagata and Toru Takeo brought their team from CARD to assist the trainees in learning the latest techniques in embryo vitrification, sperm cryopreservation, in vitro fertilization, and the new method of vitrifying oocytes. (
  • There are currently two methods of cryopreservation used in ART: slow freezing and vitrification. (
  • The use of DMSO during cell therapies has historically been a process critical step used to maintain cell viability during cryopreservation, however DMSO toxicity has been well characterized in cells throughout the human body and can cause a wide range of undesirable side effects following stem cell therapy. (
  • Cryopreservation media, or freezing media, are used to maintain viability of living cells in frozen storage. (
  • Unlike other scaffold-based cryopreservation strategies such as fiber meshes and nanofiber sheets, where the substrates must be repeatedly engineered for their use in cell cryopreservation, this paper-based method is solely based on the ready-to-use papers where cells are preserved with no significant effect on their viability and metabolic activity," said Roaa Alnemari, a former Research Assistant with Qasaimeh's lab. (
  • We observed that cryopreservation of CB in HSA, undiluted autologous human plasma and 50% diluted plasma was equivalent in terms of cell recovery and cell viability . (
  • However, the viability of leukocytes after cryopreservation varied significantly (p (
  • Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications. (
  • This closed cryopreservation/defrost system allows for sterility in addition to increased viability, recovery and safety of tissues that can be used for in vitro culture or surgical transplantation. (
  • In collaboration with I³, SLTB will organize a session on ovarian tissue cryopreservation. (
  • Alse, these authors evaluate the alternative of proactive IVF, and contemplate on the 'conditions for offering cryopreservation of ovarian tissue or oocytes' to healthy women. (
  • We argue that there are no convincing a priori moral reasons why cryopreservation of ovarian tissue or oocytes should not also be available for healthy women. (
  • Ovarian tissue cryopreservation and transplantation is a promising option for fertility preservation in pre-pubertal girls and adult patients with cancer who require immediate treatment, or who are not eligible to undergo ovarian stimulation. (
  • This review introduces various methods and strategies to improve ovarian tissue cryopreservation and transplantation outcomes, to help patients and clinicians choose the best option when considering the potential complexity of a patient's situation. (
  • We are equipped to carry out cryopreservation of mouse germ-line tissue. (
  • According to a study published April 2019 by Ellen Cristina Rivas Leonel, Carolina M. Lucci, Christiani A. Amorim, "Cryopreservation of human ovarian tissue has been increasingly applied worldwide to safeguard fertility in cancer patients, notably in young girls and women who cannot delay the onset of their treatment. (
  • BioLife Solutions, Inc.(BioLife, a leading developer, manufacturer and marketer of proprietary clinical grade cell and tissue hypothermic storage and cryopreservation freeze media, announced that its media products are embedded into the autologous cell therapy being developed by Pittsburgh-based Cook MyoSite, a subsidiary of the Cook Group, for treatment of female stress urinary incontinence. (
  • Although ovarian tissue cryopreservation is still considered as an experimental technique, several authors from around the world have reported successful and promising results. (
  • We first performed laparoscopic ovarian resection for ovarian tissue cryopreservation and then started COH on postoperative day 0 or 1 in each patient (Table 1). (
  • Dolmans MM, Marotta ML, Pirard C, Donnez J, Donnez O. Ovarian tissue cryopreservation followed by controlled ovarian stimulation and pick-up of mature oocytes does not impair the number or quality of retrieved oocytes. (
  • Please contact Leo Ennen ([email protected]) to learn more about cryopreservation, revitalization and sanitation at the NKI. (
  • Dublin And the 21 July 2022 /PRNewswire/ - file "Cryopreservation Tools Market Forecast to 2028 - Influence of COVID-19 and International Evaluation by Sort, Refrigeration Sort, Software and Finish Consumer" Report added to Present. (
  • Cryopreservation or cell freezing is the process of using low temperatures to preserve cells and tissues for future use. (
  • NutriFreez® cell freezing solutions are designed and validated for the cryopreservation of cells including stem cells, T cells, PBMCs, CHO, and Vero cells. (
  • CryoMACS® Freezing Bags and CryoMACS DMSO are the right step towards safe cryopreservation of cells at ultra-low temperatures. (
  • Are Cryopreservation and Egg Freezing the Same? (
  • Dr Silber answers questions on FB Live - is cryopreservation and egg freezing the same thing with regard to infertility treatment? (
  • In this white paper we discuss integration of high cell density cryopreservation (HCDC) in the seed train and explore key considerations for developing a cryopreservation process including choice of cryoprotectant and freezing techniques. (
  • A cryopreservation protocol, using a mixture of 30% glycerol and 70% concentrated P. persalinus cell culture, incorporating rate‐controlled freezing at −80 °C before liquid nitrogen storage, maintained a high recovery efficiency after 8 wk of storage. (
  • Cryopreservation can also be a procedure to help men maintain their fertility through freezing of their sperm. (
  • Solutions and reagents for cryopreservation: cell freezing media, Bambanker freezing and storage solutions. (
  • As NYUAD Assistant Professor of Mechanical and Biomedical Engineering Mohammad A. Qasaimeh and colleagues explain in the study, "Paper-based Cell Cryopreservation," published in the journal Advanced Biosystems , a conventional filter paper made of cellulose fibers offers a simple and robust alternative, allowing for easy loading and efficient freezing of cells. (
  • Cryopreservation is a method used to scale back cell harm that happens throughout freezing and storage of organic supplies akin to tissues, micro organism, fungi, viruses, and mammalian cells. (
  • Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissues and Organs. (
  • Cryopreservation is the use of very low temperatures to preserve structurally intact living cells and tissues. (
  • Researchers at the National Eye Institute (NEI), have developed a cryopreservation and cell recovery system designed specifically for the efficient cryopreservation, transportation and subsequent thawing of monolayers and tissues on a substrate. (
  • Genetically secure dwelling tissues and cells preserved by cryopreservation can be utilized in analysis and different biomedical functions. (
  • This review summarizes the main features of the technical development, including both achievements and controversies, and outlines the emerging assisted reproductive technique strategies based partially or predominantly on cryopreservation. (
  • The main aim of this study is to determine the effect of the straw volume (0.25 vs. 0.5 mL) on Nile tilapia sperm quality after cryopreservation. (
  • Evaluation of gilthead sea bream, Sparus aurata, sperm quality after cryopreservation in 5 ml macrotubes. (
  • An exhaustive determination of sperm quality before and after cryopreservation was investigated. (
  • It is concluded that the addition of synthetic amino acids in the semen of sheep before cryopreservation improves sperm quality and fertilization potential and can thus be added in cryopreservation protocols. (
  • Cryopreservation was efficient for the embryonic axes desiccated to 13%-15% moisture content. (
  • Cathodic amelioration of the adverse effects of oxidative stress accompanying procedures necessary for cryopreservation of embryonic axes of recalcitrant-seeded species. (
  • Although cryopreservation techniques have not yet been perfected, more than 100 people worldwide have already been cryogenically frozen after death by companies like Alcor. (
  • Mitochondrial status was affected by cryopreservation since there was a decrease in the parameters of sperm motility, ATP content (3.17 nmol ATP/10(5) spermatozoa to 1.7 nmol ATP/10(5) spermatozoa in 1:20 frozen samples) and an increase of the percentage of cells with mitochondrial depolarized membranes (11% for fresh and 27% for 1:20 frozen samples). (
  • The localized version of cryopreservation is placing a frozen bag, or a cloth with ice cubes, etc. this will reduce the pain and swelling. (
  • Cryopreservation techniques aim to minimize ice crystal formation and keep corals and their cells alive while they're being frozen. (
  • A major challenge for clinical application of pluripotent stem cell therapy for Parkinson's disease (PD) is large-scale manufacturing and cryopreservation of neurons that can be efficiently prepared with minimal manipulation. (
  • Cryopreservation is the next exciting stage of research in stem cell therapy. (
  • Based mostly on the tip consumer, the cryopreservation tools market is segmented into stem cell banks, biotechnology and pharmaceutical organizations, stem cell analysis laboratories, and others. (
  • Cord blood collection is typically depleted of red blood cells before cryopreservation to ensure high rates of stem cell recovery. (
  • Procedures for the long term passage of neurospheres and the cryopreservation of neurospheres are also provided. (
  • Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk. (
  • FUJIFILM Irvine Scientific, Inc., a world leader in the development and manufacture of serum-free and chemically defined cell culture media for bioproduction and cell therapy manufacturing, today announced that its PRIME-XV Stem FreezIS DMSO-Free * cryopreservation medium is to be used as an excipient in a FDA-authorized clinical trial conducted by Vitro Biopharma. (
  • The PTHS trial will utilize FUJIFILM Irvine Scientific's PRIME-XV Stem FreezIS DMSO-Free* cryopreservation medium as an excipient during the process. (
  • FUJIFILM Irvine Scientific's PRIME-XV Stem FreezIS DMSO-Free* cryopreservation medium is a complete and ready-to-use, chemically defined, animal component- and protein-free solution that does not contain DMSO. (
  • We are pleased to support Vitro Biopharma's clinical trial via the provision of our PRIME-XV Stem FreezIS DMSO-Free cryopreservation medium. (
  • Storage behavior and cryopreservation studies in Indian rough lemon (C" by MONY RADHIKA ROHINI, SURENDRA KUMAR MALIK et al. (
  • Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. (
  • With Expell cryogenic products you save up to 23% of your cryopreservation storage space. (
  • Additionally, you can store approximately 30% more of your cryopreservation samples on the ULT (ultralow temperature) freezer when using the 0.5mL Expell cryotubes with the right match racks for storage on ULT and 32mm cryo boxes, compared to the use of conventional 2.0mL cryotubes and 50mm boxes. (
  • Cryopreservation refers to the storage of a living organism at ultra-low-temperature such that it can be revived and restored to the same living state as before it was stored. (
  • image: The paper-based cryopreservation platform can be rolled or folded during storage to save space, and can be cut into small pieces during the retrieval of cells. (
  • The paper platform, working as a 3D shield and carrier for cells during cryopreservation, greatly simplifies the storage, management, and logistics of cell banking," said lead researcher and corresponding author Qasaimeh. (
  • However, it is unclear which are the best methodologies for cryopreservation and storage of the sample aliquots. (
  • The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines. (
  • Cryopreservation performs an essential position within the area of regenerative medication as a result of it facilitates the secure and secure storage of cells and different associated parts for a very long time. (
  • May 12, 2018 15 years have passed since the first cryopreservation procedure was conducted outside the United States. (
  • Optimizing cord blood sample cryopreservation. (
  • RÉSUMÉ La présente étude menée en Turquie a évalué l'impact de la loi rendant obligatoire le transfert d'un embryon unique en fonction de l'âge et de l'augmentation consécutive des transferts d'embryons congelés-décongelés sur l'issue de la grossesse des patientes bénéficiant d'une fécondation in vitro. (
  • Le transfert d'un embryon unique, le transfert d'embryons congelés-décongelés et le transfert de deux embryons ont été réalisés chez 5632 patientes après l'entrée en vigueur de la loi, tandis que l'approche traditionnelle par fécondation in vitro et par transferts d'embryons congelés-décongelés a été utilisée chez 6029 patientes avant le vote de cette loi. (
  • Future research should focus on in vitro production and cryopreservation of African strains of N. tanajoae in order to develop specific primers for detecting African isolates. (
  • Cryomesh is a specially fabricated mesh used as substrate in cryopreservation. (
  • They support animal component-free conditions during cryopreservation, essential to maintaining consistency when culturing cells in xeno-free and animal component-free systems. (
  • The percentage of viable cells slightly decreased after cryopreservation, however plasma membrane was affected by cryopreservation, since cells could not resist the hyperosmotic shock. (
  • The medium is validated for use in mesenchymal stromal cells and CD34+ cells, and optimized cryopreservation protocols for peripheral blood mononuclear cells and T cells have also been published. (
  • We have discovered that a microchip has an unexpected effect for cryopreservation of adhered mammalian cells. (
  • Abu Dhabi, UAE - 29 January 2020: Researchers from the Division of Engineering at NYU Abu Dhabi (NYUAD) have developed a new technique that utilizes filter paper to cryopreserve human cells, offering scientists an efficient alternative to conventional, long-term cryopreservation methods. (
  • This study examined the influence of the collection and cryopreservation of buccal cells on cell survival and DNA integrity. (
  • Cryopreservation produces several types of damage in spermatozoa, leading to fertility impairment. (
  • and 3) some recovery of viable A. zeteki spermatozoa after cryopreservation can be achieved by equilibrating the ARS-diluted samples for 5 min at 4 °C in CPA3-REY, using step-wise cooling before plunging the samples in LN2. (
  • Practices include the use of embryo re-derivation, general breeding and maintenance of genetically altered rodent colonies and the preservation and archiving of important genetically altered rodent lines by cryopreservation to ensure against loss as well as to avoid the unnecessary breeding of animals that may not be needed. (
  • Read cryopreservation, breeding and maintenance of genetically altered mice as a service non-technical summary (PDF). (
  • If cryonics were to become a reality, society would have a challenging time integrating cryonics patients, who would have been revived from cryopreservation, into the new world. (
  • Cryopreservation of the brain could be used for cryogenic suspension of those who are critically ill for possible revival in the future after medical advances. (
  • Capp Expell cryogenic products represent a new approach to space optimization in cryopreservation. (
  • Addressing the trend of cryopreservation samples being smaller and smaller, Capp developed one of the smallest cryogenic consumables available on the market - the Expell cryo tube of 0.5 mL. (
  • Optimize your cryopreservation capacity and test Expell cryogenic consumables free of charge worldwide. (
  • On the premise of cryogenic sort, the worldwide cryopreservation tools market is segmented into liquid nitrogen, oxygen, liquid helium, argon, and others. (
  • On the premise of sort, the worldwide cryopreservation tools market is segmented into freezers, pattern preparation programs, and equipment. (
  • Additionally, our review is also addressed to infertility doctors and embryologists who are marginally or actively involved in cryopreservation. (
  • Cryopreservation biotechnology has important roles for aquaculture industry and also for conservation of aquatic genetic resources. (
  • We also found that cryopreservation of CB samples in either cryovials or cryobags displayed equivalent thermal characteristics. (
  • Caboux E, Paciencia M, Durand G, Robinot N, Wozniak MB, Galateau-Salle F, Byrnes G, Hainaut P, Le Calvez-Kelm F. Impact of delay to cryopreservation on RNA integrity and genome-wide expression profiles in resected tumor samples. (
  • To accept mice for cryopreservation of your strain you will first need to submit the strain information. (
  • You can then submit another strain or proceed to 'submit mice for cryopreservation' where you can enter the individual mouse details for the mice you wish to send. (
  • Mastering cryopreservation of the brain and other organs can also improve organ transplantation. (
  • Effective multidisciplinary oncofertility strategies, involving the inclusion of a highly skilled and experienced oncofertility team that considers cryopreservation methods, thawing processes and devices, surgical procedures for transplantation, and advances in technologies, are necessary to provide high-quality care to a cancer patient. (
  • If the donor desires to use a different FDA compliant laboratory for cryopreservation, then that laboratory must agree to conduct all screening, testing, and FDA donor eligibility determination. (
  • In the encapsulation-dehydration method, encapsulated axes precultured on 0.5 M sucrose medium followed by 6 h of desiccation had the highest recovery growth of 30%-60% after cryopreservation. (
  • Cryopreservation also influenced the integrity of DNA in the comet assay. (
  • and how can we accelerate the advancement towards a standardized, widely accepted, simple and reliable new cryopreservation approach that may profoundly change the current practices in human-assisted reproductive techniques (ART). (
  • To demonstrate the feasibility of ASC, we perfuse-fixed rabbit and pig brains with a glutaraldehyde-based fixative, then slowly perfused increasing concentrations of ethylene glycol over several hours in a manner similar to techniques used for whole organ cryopreservation. (
  • In this field, sperm cryopreservation has been used for transporting of genetic material between facilities, optimal using of gametes in aquaculture, reducing risk of spreading infections, performing of hybridization studies, conserving of protecting endangered species, and also for conserving of biodiversity [ 1 , 2 ]. (
  • Moreover, we will also deal with some new, exciting developments, that - if confirmed by future studies - may further improve the efficiency of cryopreservation and the whole assisted reproduction. (