Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Cryoprotective Agents: Substances that provide protection against the harmful effects of freezing temperatures.Semen Preservation: The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Vitrification: The transformation of a liquid to a glassy solid i.e., without the formation of crystals during the cooling process.Propylene Glycol: A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations.Freezing: Liquids transforming into solids by the removal of heat.Fertility Preservation: A method of providing future reproductive opportunities before a medical treatment with known risk of loss of fertility. Typically reproductive organs or tissues (e.g., sperm, egg, embryos and ovarian or testicular tissues) are cryopreserved for future use before the medical treatment (e.g., chemotherapy, radiation) begins.Ethylene Glycol: A colorless, odorless, viscous dihydroxy alcohol. It has a sweet taste, but is poisonous if ingested. Ethylene glycol is the most important glycol commercially available and is manufactured on a large scale in the United States. It is used as an antifreeze and coolant, in hydraulic fluids, and in the manufacture of low-freezing dynamites and resins.Dimethyl Sulfoxide: A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.Tissue Preservation: The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Sperm Motility: Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.Spermatozoa: Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.Fertilization in Vitro: An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.Embryo Transfer: The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.Sperm Banks: Centers for acquiring and storing semen.Reproductive Techniques, Assisted: Clinical and laboratory techniques used to enhance fertility in humans and animals.Oocytes: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).Organ Preservation: The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Ovary: The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.Tissue Banks: Centers for acquiring, characterizing, and storing organs or tissue for future use.Blood Preservation: The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Cell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Semen Analysis: The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Blastocyst: A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.Sperm Retrieval: Procedures to obtain viable sperm from the male reproductive tract, including the TESTES, the EPIDIDYMIS, or the VAS DEFERENS.Embryo Culture Techniques: The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.Ovarian Follicle: An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS.Tissue Survival: The span of viability of a tissue or an organ.Glycerol: A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.Propylene Glycols: Derivatives of propylene glycol (1,2-propanediol). They are used as humectants and solvents in pharmaceutical preparations.Fertility: The capacity to conceive or to induce conception. It may refer to either the male or female.Primary Ovarian Insufficiency: Cessation of ovarian function after MENARCHE but before the age of 40, without or with OVARIAN FOLLICLE depletion. It is characterized by the presence of OLIGOMENORRHEA or AMENORRHEA, elevated GONADOTROPINS, and low ESTRADIOL levels. It is a state of female HYPERGONADOTROPIC HYPOGONADISM. Etiologies include genetic defects, autoimmune processes, chemotherapy, radiation, and infections.Sperm Injections, Intracytoplasmic: An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Pregnancy Rate: The ratio of the number of conceptions (CONCEPTION) including LIVE BIRTH; STILLBIRTH; and fetal losses, to the mean number of females of reproductive age in a population during a set time period.Reproductive Techniques: Methods pertaining to the generation of new individuals, including techniques used in selective BREEDING, cloning (CLONING, ORGANISM), and assisted reproduction (REPRODUCTIVE TECHNIQUES, ASSISTED).Oocyte Retrieval: Procedures to obtain viable OOCYTES from the host. Oocytes most often are collected by needle aspiration from OVARIAN FOLLICLES before OVULATION.Infertility: Inability to reproduce after a specified period of unprotected intercourse. Reproductive sterility is permanent infertility.Prenatal Injuries: Damages to the EMBRYO, MAMMALIAN or the FETUS before BIRTH. Damages can be caused by any factors including biological, chemical, or physical.Tromethamine: An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)Semen: The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.Ice: The solid substance formed by the FREEZING of water.Raffinose: A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.Embryo Implantation: Endometrial implantation of EMBRYO, MAMMALIAN at the BLASTOCYST stage.Egg Yolk: Cytoplasm stored in an egg that contains nutritional reserves for the developing embryo. It is rich in polysaccharides, lipids, and proteins.Sperm Count: A count of SPERM in the ejaculum, expressed as number per milliliter.Infertility, Female: Diminished or absent ability of a female to achieve conception.Dry Ice: A solid form of carbon dioxide used as a refrigerant.Acrosome: The cap-like structure covering the anterior portion of SPERM HEAD. Acrosome, derived from LYSOSOMES, is a membrane-bound organelle that contains the required hydrolytic and proteolytic enzymes necessary for sperm penetration of the egg in FERTILIZATION.Blastomeres: Undifferentiated cells resulting from cleavage of a fertilized egg (ZYGOTE). Inside the intact ZONA PELLUCIDA, each cleavage yields two blastomeres of about half size of the parent cell. Up to the 8-cell stage, all of the blastomeres are totipotent. The 16-cell MORULA contains outer cells and inner cells.Cleavage Stage, Ovum: The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.Ejaculation: The emission of SEMEN to the exterior, resulting from the contraction of muscles surrounding the male internal urogenital ducts.TrehaloseSucrose: A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.Extreme Cold: Below normal weather temperatures that may lead to serious health problems. Extreme cold is a dangerous situation that can bring on health emergencies in susceptible people.Pregnancy Outcome: Results of conception and ensuing pregnancy, including LIVE BIRTH; STILLBIRTH; SPONTANEOUS ABORTION; INDUCED ABORTION. The outcome may follow natural or artificial insemination or any of the various ASSISTED REPRODUCTIVE TECHNIQUES, such as EMBRYO TRANSFER or FERTILIZATION IN VITRO.Live Birth: The event that a FETUS is born alive with heartbeats or RESPIRATION regardless of GESTATIONAL AGE. Such liveborn is called a newborn infant (INFANT, NEWBORN).Zona Pellucida: A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.Cell Culture Techniques: Methods for maintaining or growing CELLS in vitro.Gentiana: A plant genus of the family Gentianaceae whose members contain SECOIRIDOIDS and have been used in TRADITIONAL MEDICINE for suppressing INFLAMMATION.Thermal Conductivity: The heat flow across a surface per unit area per unit time, divided by the negative of the rate of change of temperature with distance in a direction perpendicular to the surface. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Spermatogonia: Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.Insemination, Artificial: Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION.Infertility, Male: The inability of the male to effect FERTILIZATION of an OVUM after a specified period of unprotected intercourse. Male sterility is permanent infertility.Testis: The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.Organ Preservation Solutions: Solutions used to store organs and minimize tissue damage, particularly while awaiting implantation.Preservation, Biological: The process of protecting various samples of biological material.Embryonic Development: Morphological and physiological development of EMBRYOS.Oligospermia: A condition of suboptimal concentration of SPERMATOZOA in the ejaculated SEMEN to ensure successful FERTILIZATION of an OVUM. In humans, oligospermia is defined as a sperm count below 20 million per milliliter semen.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Specimen Handling: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.Ovulation Induction: Techniques for the artifical induction of ovulation, the rupture of the follicle and release of the ovum.Oocyte Donation: Transfer of preovulatory oocytes from donor to a suitable host. Oocytes are collected, fertilized in vitro, and transferred to a host that can be human or animal.Fertilization: The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE.Antelopes: Any of various ruminant mammals of the order Bovidae. They include numerous species in Africa and the American pronghorn.Morula: An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.Zygote: The fertilized OVUM resulting from the fusion of a male and a female gamete.Cocos: A plant genus of the family ARECACEAE. It is a tropical palm tree that yields a large, edible hard-shelled fruit from which oil and fiber are also obtained.Osmotic Pressure: The pressure required to prevent the passage of solvent through a semipermeable membrane that separates a pure solvent from a solution of the solvent and solute or that separates different concentrations of a solution. It is proportional to the osmolality of the solution.Phascolarctidae: A family of marsupials in the order Diprotodontia, native to Australia and possessing vestigial tails. There is a single living genus and species: Phascolarctos cinereus, the koala.Embryonic and Fetal Development: Morphological and physiological development of EMBRYOS or FETUSES.Tissue Culture Techniques: A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.Tissue and Organ Harvesting: The procedure of removing TISSUES, organs, or specimens from DONORS for reuse, such as TRANSPLANTATION.Aquaporin 3: Aquaporin 3 is an aquaglyceroporin that is expressed in the KIDNEY COLLECTING DUCTS and is constitutively localized at the basolateral MEMBRANE.Viviparity, Nonmammalian: The capability of bearing live young (rather than eggs) in nonmammalian species. Some species of REPTILES and FISHES exhibit this.Cold Temperature: An absence of warmth or heat or a temperature notably below an accustomed norm.Centrifugation: Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Single Embryo Transfer: The techniques used to select and/or place only one embryo from FERTILIZATION IN VITRO into the uterine cavity to establish a singleton pregnancy.Teratology: A branch of embryology for the study of congenital malformations and developmental abnormalities.Biological Specimen Banks: Facilities that collect, store, and distribute tissues, e.g., cell lines, microorganisms, blood, sperm, milk, breast tissue, for use by others. Other uses may include transplantation and comparison of diseased tissues in the identification of cancer.Mice, Inbred ICRCell Membrane Permeability: A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.Pregnancy, Multiple: The condition of carrying two or more FETUSES simultaneously.Ovarian Hyperstimulation Syndrome: A complication of OVULATION INDUCTION in infertility treatment. It is graded by the severity of symptoms which include OVARY enlargement, multiple OVARIAN FOLLICLES; OVARIAN CYSTS; ASCITES; and generalized EDEMA. The full-blown syndrome may lead to RENAL FAILURE, respiratory distress, and even DEATH. Increased capillary permeability is caused by the vasoactive substances, such as VASCULAR ENDOTHELIAL GROWTH FACTORS, secreted by the overly-stimulated OVARIES.

Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay. (1/2910)

The sperm chromatin structure assay (SCSA) is a flow cytometric (FCM) technique which exploits the metachromatic properties of Acridine Orange to monitor the susceptibility of sperm chromatin DNA to in-situ acid denaturation. SCSA was used to study the chromatin structure variations of human spermatozoa in semen, both before and after swim-up and after cryopreservation. Semen samples were provided by 19 healthy normozoospermic subjects attending pre-marriage checks. Each sample was divided into three aliquots: the first aliquot was evaluated without further treatment, the second underwent swim-up, and the third was stored according to standard cryopreservation techniques in liquid nitrogen at -196 degrees C. Samples were also analysed by light and fluorescence microscopy (after Acridine Orange staining to evaluate the number of green fluorescent sperm heads), and by computer-assisted semen analysis. The results showed that post-rise spermatozoa represent a subpopulation characterized by a general improvement of the morphological (reduction of the percentage of abnormal forms and heads, increase of the green head sperm percentage) and kinetic parameters. This subpopulation also exhibited improved chromatin structure properties, confirming that these cells have the best structural and functional characteristics, indicative of optimal fertilizing ability. On the other hand, overall sperm quality deteriorates after cryopreservation. When thawed spermatozoa underwent an additional swim-up round, a general improvement of nuclear maturity was seen in the post-rise spermatozoa.  (+info)

Arterial damage induced by cryopreservation is irreversible following organ culture. (2/2910)

OBJECTIVES: The aim of the present study was to investigate the changes which occur to the arterial wall following cryopreservation and thawing and to determine whether these changes are reversible after a week of culture in an organ bath. MATERIALS AND METHODS: Rat iliac arterial segments were cryopreserved. Once thawed, the arterial segments were cultured for a period of 0, 1, 2, 4 or 7 days. Freshly isolated rat iliac vessels cultured for 7 days served as the control group. Evaluation was made of ultrastructural changes, the expression of metalloproteinase activity (MMP-1, MMP-3 and MMP-9) and the apoptotic state of cells. RESULTS: The freezing-thawing process induced damage to the arterial segments compared to fresh control vessels. After 1 week of culture, arteries showed a high degree of tissue degeneration. Only a few individual endothelial cells remained on the luminal surface. There was a gradual increase in the proportion of apoptotic cells. The sequential expression of MMP-1 during the first 2 days and subsequent expression of MMP-3 and MMP-9 were of most significance. CONCLUSIONS: Cryopreservation induced damage to the vessels which could not be reversed by organ culture. The changes observed in the expression of metalloproteinases may be indicative of the degenerative process which occurs in the extracellular matrix.  (+info)

Effect of cryopreservation on cytochrome P-450 enzyme induction in cultured rat hepatocytes. (3/2910)

In the present study, we evaluated the inducibility of cytochrome P-450 (CYP) CYP1A, CYP2B, CYP3A, and CYP4A by beta-naphthoflavone, phenobarbital, dexamethasone, and clofibric acid, respectively, in primary hepatocyte cultures prepared from both fresh and cryopreserved rat hepatocytes. Rat hepatocytes were successfully thawed and cultured after cryopreservation in liquid nitrogen for up to 1 month. Percentage of total recovery, viable cell recovery, and final viability of the cells were 68%, 72%, and 85%, respectively. Regardless of whether they were cryopreserved or not, cultured hepatocytes exhibited near-normal morphology. Treatment of cryopreserved hepatocytes with beta-naphthoflavone caused an 8-fold increase in 7-ethoxyresorufin O-dealkylase (CYP1A1/2) activity, with an EC50 of 1.5 microM; treatment with phenobarbital caused a 26-fold increase in 7-pentoxyresorufin O-dealkylase (CYP2B1/2) activity, with an EC50 of 10 microM; treatment with dexamethasone caused a 10-fold increase in testosterone 6beta-hydroxylase (CYP3A1/2) activity, with an EC50 of 1.3 microM, whereas treatment with clofibric acid caused a 3-fold increase in lauric acid 12-hydroxylase (CYP4A1-3) activity, with an EC50 of 170 microM. The induction of CYP1A, CYP2B, CYP3A, and CYP4A enzymes by these inducers was confirmed by Western immunoblotting. The patterns of P-450 induction in cryopreserved rat hepatocytes, in terms of concentration response, reproducibility, magnitude, and specificity of response, were similar to those observed in freshly isolated hepatocytes. Additionally, the magnitude and specificity of induction was similar to that observed in vivo in rats. In conclusion, under the conditions examined, cryopreserved rat hepatocytes appear to be a suitable in vitro system for evaluating xenobiotics as inducers of P-450 enzymes.  (+info)

Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. (4/2910)

When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane after different cryostorage protocols: i.e. 10% (v/v) glycerol; sperm maintenance medium (MM); freezing medium TEST yolk buffer (TYB); or cryostorage without protection (cryoshock). Using a combination of two fluorescent dyes, annexin V and propidium iodide (PI), led to three groups of spermatozoa being identified: (i) viable spermatozoa (annexin V-negative and PI-negative); (ii) dead spermatozoa (annexin V-positive and PI-positive); and (iii) cells with impaired but integer plasma membrane (annexin V-positive and PI-negative). The percentage of vital annexin V-negative spermatozoa increased significantly (P < 0.05) from spermatozoa treated by cryoshock (15.0+/-1.2%) to spermatozoa cryopreserved by TYB (26.6+/-2.2%) via cryopreservation by 10% (v/v) glycerol (19.9+/-1.6%) and by MM (22.2 1.8%) and was associated with the percentage of motile spermatozoa (17.6+/-3.4% by glycerol; 19.6+/-3.7% by MM and 22.6+/-3.9% by TYB; P = 0.0001). Of the spermatozoa, 12-22% were annexin V-positive even though they did not bind to PI, indicating viability before as well as after cryostorage. The percentage of vital annexin V-positive spermatozoa was significantly correlated with different sperm motility parameters (velocity straight linear, r = 0.601, P = 0.018; percentage of linearly motile spermatozoa: r = 0.549, P = 0.034). We, therefore, concluded that annexin V-binding is more sensitive in detecting a deterioration of membrane functions than PI staining, and that a considerable percentage of spermatozoa might have dysfunctional plasma membranes besides dead or moribund cells. Of the cryopreservation protocols tested, TYB yielded the most viable spermatozoa. Therefore, we advocate the use of the annexin V-binding assay for the evaluation of the quality and integrity of spermatozoa.  (+info)

Freezer anthropology: new uses for old blood. (5/2910)

Archived blood fractions (plasma, settled red cells, white cells) have proved to be a rich and valuable source of DNA for human genetic studies. Large numbers of such samples were collected between 1960 and the present for protein and blood group studies, many of which are languishing in freezers or have already been discarded. More are discarded each year because the usefulness of these samples is not widely understood. Data from DNA derived from 10-35-year-old blood samples have been used to address the peopling of the New World and of the Pacific. Mitochondrial DNA haplotypes from studies using this source DNA support a single wave of migration into the New World (or a single source population for the New World), and that Mongolia was the likely source of the founding population. Data from Melanesia have shown that Polynesians are recent immigrants into the Pacific and did not arise from Melanesia.  (+info)

Preparation of endometrium for egg donation. (6/2910)

Nowadays oocyte donation is a well established method of assisted reproduction and offers the unique opportunity to treat patients with various clinical indications, with or without ovarian function, in a novel way. In women with ovarian failure, artificial menstrual cycles are required before proceeding to oocyte donation. Oestrogen may be delivered in the form of oral tablets, transdermal patches in order to bypass the gastrointestinal tract thus avoiding first pass metabolism and by vaginal application. Our regimen is oestradiol valerate given in various concentrations, in order to mimic the regular cyclic fluctuations throughout the cycle. Progesterone may be administered in the form of oral tablets, intravaginal suppositories or rings and i.m. injections. Our results, as of most other groups, strongly support the vaginal route of progesterone administration. In women with retained ovarian function, synchronization of donor-recipient cycle presents a special problem, as there is strong evidence that a temporal window of maximal endometrial receptivity exists. Cryopreservation of donated embryos may be used to overcome the problem, but this approach has the important drawback of embryonic loss occurring after freezing and thawing. The method of choice is the administration of gonadotrophin-releasing hormone agonists (GnRHa) to render the patients functionally agonadal in order to circumvent cycle asynchrony between the donor and recipient.  (+info)

Turner's syndrome and pregnancies after oocyte donation. (7/2910)

A total of 20 clinical pregnancies was achieved among 18 women with Turner's syndrome who were treated in an oocyte donation programme. The oocytes were donated by voluntary unpaid donors. A mean of 1.8 embryos per transfer was given to each recipient by way of 28 fresh and 25 frozen embryo transfers. With fresh and frozen embryos, 13 and seven pregnancies respectively were achieved. The clinical pregnancy rate per fresh embryo transfer was 46%, and the implantation rate 30%, being similar to the corresponding rates among our oocyte recipients with primary ovarian failure in general. The corresponding rates with frozen embryos were 28 and 19%. Of these pregnancies, 40% ended in miscarriage. This high rate may be explained by uterine factors. Six women were hypertensive during pregnancy, a rate comparable with that in other oocyte donation pregnancies. All these women delivered by Caesarean section. Pregnancy and implantation rates after oocyte donation were high in women with Turner's syndrome, but the risk of cardiovascular and other complications is high. Careful assessment before and during follow-up of pregnancy are important. Transfer of only one embryo at a time to avoid the additional complications caused by twin pregnancy is recommended.  (+info)

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing. (8/2910)

Uncontrolled-rate freezing techniques represent an attractive alternative to controlled-rate cryopreservation procedures which are time-consuming and require high-level technical expertise. In this study, we report our experience using uncontrolled-rate cryopreservation and mechanical freezer storage at -140 degrees C. Twenty-eight PBPC samples (10 cryovials, 18 freezing bags) from 23 patients were cryopreserved in a cryoprotectant solution composed of phosphate-buffered saline (80%, v/v) supplemented with human serum albumin (10%, v/v) and dimethylsulfoxide (10%, v/v). The cryopreservation procedure required on average 1.5 h. The mean (+/- s.e.m.) storage time of cryovials and bags was 344+/-40 and 299+57 days, respectively. Although cell thawing was associated with a statistically significant reduction of the absolute number of nucleated cells (vials: 0.3x10(9) vs. 0.2x10(9), P< or =0.02; bags: 14x10(9) vs. 11x10(9), P< or =0.0003), the growth of committed progenitors was substantially unaffected by the freezing-thawing procedure, with mean recoveries of CFU-Mix, BFU-E, and CFU-GM ranging from 60+/- 29% to 134+/-15%. Mean recoveries of LTC-IC from cryovials and bags were 262+/-101% and 155+/-27% (P< or =0.2), respectively. In 14 out of 23 patients who underwent high-dose chemotherapy and PBPC reinfusion, the pre-and post-freezing absolute numbers of hematopoietic progenitors cryopreserved in bags were compared. A significant reduction was detected for CFU-Mix (11 vs. 7.4x10(5)), but no significant loss of BFU-E (180 vs. 150x10(5)), CFU-GM (400 vs. 290x10(5)) and LTC-IC (15 vs. 16x10(5)) could be demonstrated. When these patients were reinfused with uncontrolled-rate cryopreserved PBPC, the mean number of days to reach 1x10(9)/l white blood cells and 50x10(9)/l platelets were 9 and 13, respectively. In conclusion, the procedure described here is characterized by short execution time, allows a substantial recovery of primitive and committed progenitors and is associated with prompt hematopoietic recovery following myeloablative therapy even after long-term storage.  (+info)

Embryo cryopreservation involves the generation of pre-implantation embryos using males provided by the investigator and the controlled rate cooling of collected embryos to -30°C followed by rapid freezing of embryos in liquid nitrogen to -196°C.. While embryo cryopreservation is the gold standard for preserving mouse germplasm, not all mouse strains are suitable for embryo cryopreservation. However, we have been successful at cryopreserving embryos from several different mouse strains.. Embryos for cryopreservation can be obtained in two basic ways: ...
There are many benefits of oocyte and embryo cryopreservation. When used as part of the IVF treatment process, cryopreservation can increase the likelihood of achieving pregnancy for many patients. Cryopreservation also allows couples to continue to see their families grow as IVF cycles can be repeated, years later, from the same group of eggs originally recovered for treatment.. During an IVF cycle, between 10 and 30 eggs can typically be recovered from the ovary. While it is likely that not all of these eggs will be deemed appropriate for cryopreservation, the process frequently allows couples to save a number of eggs for multiple conception attempts. In some cases, this can lead to successful treatment without the use of fertility drugs. It also reduces the likelihood that another egg retrieval procedure will be required. The number of embryos transferred depends on the number of eggs available, age, and other factors that are unique to each patient. In some cases, cryopreservation makes ...
Oocyte Cryopreservation abroad in India offers info on cost Oocyte Cryopreservation India,Oocyte Cryopreservation IVF Doctors &hospitals India.
OBJECTIVE: During IVF, non-transferred embryos are usually selected for cryopreservation on the basis of morphological criteria. This investigation evaluated an application for array comparative genomic hybridization (aCGH) in assessment of surplus embryos prior to cryopreservation. METHODS: First-time IVF patients undergoing elective single embryo transfer and having at least one extra non-transferred embryo suitable for cryopreservation were offered enrollment in the study. Patients were randomized into two groups: Patients in group A (n=55) had embryos assessed first by morphology and then by aCGH, performed on cells obtained from trophectoderm biopsy on post-fertilization day 5. Only euploid embryos were designated for cryopreservation. Patients in group B (n=48) had embryos assessed by morphology alone, with only good morphology embryos considered suitable for cryopreservation. RESULTS: Among biopsied embryos in group A (n=425), euploidy was confirmed in 226 (53.1%). After fresh single embryo
Populations of Redside Dace Clinostomus elongatus have declined in many areas across the speciesNorth American range. Therefore, the development of sperm cryopreservation technology would provide an invaluable means of preserving genetic diversity in populations that are in imminent danger of extirpation.We developed cryopreservation protocols by testing the effects of diluent (buffered sperm motility-inhibiting saline solution [BSMIS]; BSMIS + glycine; sucrose; and Hanks balanced salt solution [HBSS]), cryoprotectant (dimethyl sulfoxide [DMSO]; propylene glycol [PG]; N,N-dimethylacetamide [DMA]; and methanol), freezing rate (1, 5, and 10◦C/min), and male-to-male variation on sperm quality. Incubating sperm in extenders affected motility;BSMIS + glycine + methanol,BSMIS + glycine + PG, and HBSS + methanol were the only treatments for which motility was not significantly different from that of fresh sperm. Sperm frozen with sucrose had higher motility than sperm frozen with BSMIS + glycine, ...
Development of effective cryopreservation protocols will be essential to realizing the potential for clinical application of neural stem and progenitor cells. Current cryopreservation protocols have been largely employed in research, which does not require as stringent consideration of viability and sterility. Therefore, these protocols involve the use of serum and protein additives, which can potentially introduce contaminants, and slow cooling with DMSO/glycerol-based cryopreservation solutions, which impairs cell survival. We investigated whether serum- and protein-free vitrification is effective for functional cryopreservation of neurosphere cultures of neural stem or progenitor cells. To protect the samples from introduction of other contaminants during handling and cryostorage, an original straw-in-straw method (250 l sterile straw placed in 500 l straw) for direct immersion into liquid nitrogen and storing the samples was also introduced. The protocol employed brief step-wise ...
Find the best embryo cryopreservation doctors in Mumbai. Get guidance from medical experts to select embryo cryopreservation specialist in Mumbai from trusted hospitals - credihealth.com
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
Background Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols in different species that would allow for an efficient storage of semen samples for long periods of time, ensuring its viability. Nowadays, it is widely known that an important individual component exists in the cryoresistance of semen, and efforts are aimed at identifying those sperm characteristics that may allow us to predict this cryoresistance. This knowledge would lead, ultimately, to the design of optimized freezing protocols for the sperm characteristics of each male. Methodology/Principal Findings We have evaluated the changes that occur in the sperm head dimensions throughout the cryopreservation process. We have found three different patterns of response, each of one related to a different sperm quality at thawing. We have been able to characterize males based on these patterns. For each male, its pattern remained constant among
Vitrification is an ultra‐rapid cryopreservation technique that provides excellent survivability by using optimal concentrations of cryoprotectants used in a step‐wise process supporting rapid dehydration of the oocyte. The dehydrated oocyte is loaded into a thin storage device that will facilitate ultra‐rapid cooling of the oocyte when plunged into liquid nitrogen - eliminating concerns of damaging ice‐crystal formation associated with traditional slow‐freezing procedures. Thaw solutions are formulated to support rehydration through the rapid warming process that follows vitrification to optimize cellular survival.. Irvine Scientifics vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. These solutions support implantation and pregnancy rates comparable to those of fresh cycles.. ...
Ovarian tissue cryopreservation and transplantation is a real option to preserve and restore fertility in young cancer patients. However, there is a concern regarding the possible presence of malignant cells in the ovarian tissue, which could lead to recurrence of the primary disease after reimplantation. A review of the existing literature was done to evaluate the risk of transplanting malignant cells in case of the main malignant indications for ovarian tissue cryopreservation. For ovarian tissue from patients with hematologic malignancies, it is of paramount importance to identify minimal residual disease before ovarian tissue transplantation. Indeed, these pathologies, reviewed here in detail, are considered to be most at risk of ovarian metastasis ...
The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for future use.
Global Stem Cells Cryopreservation Equipments Market: This market research report focuses on Past-Current Size, Price, Trends, Shares, Segment & Forecast
Jenderek, M.M.; Ambruzs, B.; Tanner, J.; Holman, G.; Ledbetter, C.; Postman, J.; Ellis, D.; Leslie, C. 2014. Extending the dormant bud cryopreservation method to new tree species. In: Reed, B.M. (ed). Proceedings of the International Conference. 2. International Symposium on Plant Cryopreservation. Fort Collins (USA). 11-14 Aug 2013. Conference Paper International Society for Horticultural Science (ISHS). ISBN 978-94-62610-27-9. pp. 133-136. Acta Horticulturae. ISSN 0567-7572. no.1039 ...
CryoSearch was created by BioCision in order to help standardize the field of cryopreservation through the sharing of protocols and encouraging discussion about protocols and methods. At BioCision, our mission is to standardize sample handling. While our products help do that, products are only one part of reducing variability. The other part is how those products get used; in other words, protocols. Since we have somewhat of a specialty in cryopreservation, and there arent any other resources like CryoSearch, we thought this would be a great place to start.. Like the site? Wed love for you to check out our cryopreservation products as well. We have leak-proof and barcoded cryovials, a line of containers for slow, controlled-rate cell freezing, and tube racks that ensure precise temperature control and can also be used for snap-freezing as they are liquid-nitrogen compatible. We have a bunch of other products as well, all designed to help make your science more reliable.. Even if youre not in ...
This advisory is written for those who would like to understand the processes of mouse strain cryopreservation, why it is important and how to take advantage of the services offered. The most efficient method to protect mouse strains is by cryopreservation of gametes or embryos. Cryopreservation, and especially re-animation from frozen material, is not a trivial exercise, nor is the establishment of a cryopreservation and IVF laboratory cheap. There are numerous laboratories around the world that offer these services.
If during your egg donation procedure with fresh or frozen donor eggs more embryos were obtained than are going to be transferred into the uterus, these embryos, if of good quality, can be frozen. These frozen embryos are property of the couple or a single woman and can be used for the next attempt or a next pregnancy. They may be required if you have not got pregnant in the fresh try or if you have got pregnant and want a brother or sister for your first child several years later.. The benefit of cryopreservation is that a woman has an additional chance to conceive without new stimulation procedure and follicular puncture in the donor. Also new sperm collection is not required. Hence the transfer of frozen embryos is much less time consuming and very cost effective. It requires only one day visit for a woman and does not require a visit of a man. Preparation of uterus to the embryo transfer does not differ much from the preparation to the fresh embryo transfer.. Embryo cryopreservation is the ...
Embryo cryopreservation or embryo freezing is a method used to preserve embryos by cooling and storing them at low temperatures (-196°C). They can then be thawed at a future date and transferred to the uterus, providing additional opportunity for achieving conception.. As part of the usual process of in vitro fertilization, multiple eggs may be stimulated to grow, be recovered from the ovary and become fertilized. This may result in additional embryos in excess of the number that a couple would desire to have transferred back to the uterus at one time. If the additional embryos are of sufficiently good quality to undergo the process of cryopreservation, this can be performed in order to provide another opportunity for embryo transfer. Depending on the embryo stage at the time of freezing, between 60-90% survive freeze/thaw process resulting in a future pregnancy.. If the IVF fresh embryo transfer does not result in pregnancy, the frozen embryos can be subsequently thawed and transferred to the ...
Cryopreserved donor lymphocyte infusion (DLI) products are manufactured and administered to treat relapse after allogeneic hematopoietic stem cell transplantation. Reported clinical responses to DLIs vary broadly, even within the same group of patients. While there is an implicit recognition of the fact that different manufacturing protocols may have specific effects on different cell types, cryopreservation protocols are frequently derived from our experience in the cryopreservation of stem cell products and do not account for the heterogeneous functional nature of DLI T-cell populations. Here, we report the results of a prospective, multicenter trial on the effect of four different cryopreservation solutions that were used to freeze DLIs compared to control DLIs that were refrigerated overnight ...
Abstract: There are many reasons why cryopreservation of gametes are important: 1) maintenance of genetic diversity in domestic and wild species populations (Wildt 1992; Wildt 1997; Critser and Russell 2000), 2) facilitating the distribution of genetically superior domestic species lines, 3) treatment of human infertility (Kuczynski et al. 2001; Ranganathan et al. 2002; Tash et al. 2003; Agarwal et al. 2004; Nalesnik et al. 2004), and 4) genetic banking of genetically modified animal models of human health and disease (Critser and Russell 2000; Knight and Abbott 2002). Although cryopreservation of gametes have been routine in many other livestock industries such as the dairy industry. Cryopreservation in the swine industry is still in it infancy. Birth of live offspring has been reported from cryopreserved sperm and embryos, but success is still extremely low. From an industry perspective the low success rate has too much of an economic impact that the integration of the technology has been ...
Clinica Mar&Gen offers Testicular Biopsy Cryopreservation procedures starting from $13,400 and it is specialized in Reproductive Medicine treatments.
Taconic offers both embryo and sperm cryopreservation. Taconic also offers revitalization of cryopreserved lines. Learn more today.
An undesired side effect of cancer treatment is potential subfertility or infertility. Timely cryopreservation of semen is the best modality to ensure fertility. This retrospective data analysis established the usage rate of cryopreserved semen from cancer patients. Pubertal and post-pubertal patients who could become infertile as a result of cancer ... read more (treatment) were offered the option to cryopreserve semen prior to treatment. Of the 898 patients who cryopreserved their semen in our hospital, 96 (10.7%) used this for assisted reproductive technology. The live birth rates for intrauterine insemination, in-vitro fertilization, intracytoplasmic sperm injection and cryopreserved embryo transfer were 13%, 29%, 32% and 17%, respectively. Of all couples involved, 77% achieved parenthood, i.e. 60 of the 78 patients (with complete follow-up) fathered at least one child. show less ...
With Europe Biobank Week 2018 in Antwerp, Belgium coming up (September 4-7th) people will be reviewing the available information on the subject. A definitive book is published by Springer - and of the fourteen chapters, chapter five, The Future of Cell Preservation Strategies by John M. Baust et al will probably interest our customers.. Dr Baust introduces this subject: ... cryopreservation is often viewed as an old school discipline yet modern cryopreservation is undergoing another scientific and technology development growth phase. In this regard, todays cryopreservation processes and cryopreserved products are found at the forefront of research in the areas of discovery science, stem cell research, diagnostic development and personalised medicine. As the utilisation of cryopreserved cells continues to increase, the demands placed on the biobanking industry are increasing and evolving at an accelerated rate. No longer are samples providing for high immediate post-thaw viability ...
Excessive reactive oxygen species generation during sex sorting and cryopreservation of stallion sperm leads to DNA fragmentation, lipid peroxidation, and motility loss. In this study we investigated whether antioxidant supplementation during sex sorting and cryopreservation could ameliorate the effects of reactive oxygen species on stallion sperm. In experiment 1, the postthaw characteristics of stallion sperm (N = 9) cryopreserved in the presence or absence of catalase (200 U/mL), cysteine (0.2 mg/mL), or quercetin (0.15 mM) was examined. Motility and acrosome integrity were assessed at 0, 1, and 3 hours after thawing. The sperm chromatin structure assay (SCSA; detectable DNA fragmentation index [DFI], mean DFI, and DFI) was used to assess DNA integrity immediately after thawing. Quercetin increased the total postthaw motility (25.3% vs. 20.9%; P | 0.05), but there was no beneficial effect of catalase or cysteine. Based on these results, the effect of quercetin during cryopreservation on the postthaw
DALLAS, Texas (SEND2PRESS NEWSWIRE) -- Women delaying childbearing or requiring fertility preservation prior to undergoing treatment now have an option with the launch of oocyte cryopreservation at Fertility Specialists of Texas. Now there is an option - egg freezing - that enables women to have eggs (also known as oocytes) aspirated before undergoing cancer treatment, and frozen for later use. - News from Fertility Specialists of Texas, issued by Send2Press Newswire
Background Simple and effective cryopreservation of human oocytes would have an enormous impact on the financial and ethical constraints of human assisted reproduction. Recently, studies have demonstrated the potential for cryopreservation in an ice-free glassy state by equilibrating oocytes with high concentrations of cryoprotectants (CPAs) and rapidly cooling to liquid nitrogen temperatures. A major difficulty with this approach is that the high concentrations required for the avoidance of crystal formation (vitrification) also increase the risk of osmotic and toxic damage. We recently described a mathematical optimization approach for designing CPA equilibration procedures that avoid osmotic damage and minimize toxicity, and we presented optimized procedures for human oocytes involving continuous changes in solution composition. Methods Here we adapt and refine our previous algorithm to predict piecewise-constant changes in extracellular solution concentrations in order to make the predicted ...
Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request ...
Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use.
Germinal vesicle and metaphase I oocytes should be matured in vitro before cryopreservation to optimize the reproductive potential of all retrieved oocytes.
The use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential ...
In the past two decades, laboratories around the world have produced thousands of mutant, transgenic, and wild-type zebrafish lines for biomedical research. Although slow-freezing cryopreservation of zebrafish sperm has been available for 30 years, current protocols lack standardization and yield inconsistent post-thaw fertilization rates. Cell cryopreservation cannot be improved without basic physiological knowledge, which was lacking for zebrafish sperm. The first goal was to define basic cryobiological values for wild-type zebrafish sperm and to evaluate how modern physiological methods could aid in developing improved cryopreservation protocols. Coulter counting methods measured an osmotically inactive water fraction (Vb) of 0.37+/-0.02 (SEM), an isosmotic cell volume (V(o)) of 12.1+/-0.2mum(3) (SEM), a water permeability (L(p)) in 10% dimethyl sulfoxide of 0.021+/-0.001(SEM)mum/min/atm, and a cryoprotectant permeability (P(s)) of 0.10+/-0.01 (SEM)x10(-3)cm/min. Fourier transform infrared ...
Attain higher post-thaw sperm recovery with the only CE marked and FDA approved medium with TEST-yolk buffer (TYB) and glycerol for freezing sperm.
I15 Should Cryoprotectants be Formulated According to the Mouse Genome?. Mike Legge, Mat Byers and Stephen Bird. Molecular Embryology Group, Department of Biochemistry, University of Otago, PO BOX 56 Dunedin, New Zealand. The use of penetrating cryporotectants such as dimethyl sulphoxide and I,2-propanediol is well established for mouse embryo cryopreservation. However, the sensitivity of both oocytes and embryos to adverse effects of both cryoprotectants and the freezing process is well documented (Duliquost et al. 1999). For mouse genome cryopreservation this is especially important as there is a correlation between mouse embryo genotype and cryopreservation success. (Schmidt et al. 1985). With the increasing necessity to archive mouse genomes by embryo and gamete cryopreservation the improvement in cryoprotectant formulations is becoming increasingly important. Recently we identified the non-enzymatic formation of formaldehyde in cryoprotectant solutions and that at the micro-molar ...
Mouse half ovaries were cryopreserved and orthotopically transplanted into ovariectomized recipients genetically identical to ovary donors except for the coat color gene. Fertility was reestablished in 57% of the female recipients, which became pregnant in an average of 40 days after transplantation of frozen-thawed half ovaries. These experiments demonstrate that ovary cryopreservation can be a very useful option for banking mouse germplasm, or managing subfertile animal colonies, when embryo or sperm freezing cannot be used or is not cost effective.
Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have darker cytoplasm than their in vivo-derived counterparts because of higher lipid accumulation. High lipid accumulation is associated with impaired embryo quality. Forskolin is an adenylate cyclase activator that regulates cAMP levels in cells and has been shown to induce lipolysis in IVP embryos. L-carnitine is required for transport of fatty acids from the intermembrane space of the mitochondria into the mitochondrial matrix to support the process of β-oxidation, and enhances ATP production. We hypothesized that the lipid content of in vivo-produced and IVP Jersey embryos is higher than respective Holstein embryos and that forskolin + L-carnitine would reduce lipid content of IVP embryos and vitrification with
It is well known that laser-assisted hatching (LAH) is the most popular and ideal embryo hatching technology, but the relevance to pregnancy outcomes of cryopreserved-thawed embryo transfer (ET) is...
Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request ...
The aim of this study was to compare the effects of cryopreservation at approximately -196 °C in liquid nitrogen (N) and freezing at approximately -20 °C in a freezer, on the viability and survival of eight different mastitogenic bacteria inoculated in milk. Bacteria were frozen at approximately -20 °C in a freezer and cryopreserved at approximately -196 °C in liquid nitrogen. An effective preservation method was needed for follow-up samples from cows identified in the South African National Milk Recording Scheme (NMRS) with somatic cell counts above 250 000 cells/mL milk. The organisation responsible for sample collection of the NMRS milk samples also provides producers with liquid nitrogen for their semen flasks at the collection sites. This existing mode of storage and transport could therefore be utilised. Ten samples of each organism were thawed and cultured bi-weekly until week 18 for both temperature treatments. An additional sampling was performed at week 30 for samples frozen at ...
Recently, Ive heard of something called Oocyte cryopreservation, where a (fertilized, I think) egg from a woman is extracted, frozen and later thawed and reinserted into the woman to delay pregnancy.. Now, this is just an idea, I dont know if this is actually possible, but can this frozen egg be implanted into a different woman, who isnt the original owner of the egg? If yes, whose genes would the child inherit? Would it get genes from all three parents, or just from the original owner of the sperm and egg?. ...
A device for use in cryopreservation of blood vessels comprising a pair of styles insertable into the ends of a dissected blood vessel segment. The styles are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant.
Morphological changes in equine, bovine, and canine erythrocytes, when interacting with 20% dimethylsulfoxide (DMSO) and 30% polyethylene oxide with molecular mass of 1500 (PEO-1500) solutions, as well as after cell cryopreservation at the presence of the mentioned cryoprotectants have been studied in this research using light microscopy. Frozen-thawed erythrocytes of all animal species were established to be transformed into echinocytes under DMSO penetrative cryoprotectant effect and into stomatocytes under a non-penetrative one. The state of animal frozen-thawed erythrocytes when transferring them into physiological conditions was analysed.. ...
The present Core C (Cryopreservation and Stem Cell Biology Core), evolved from a predecessor devoted strictly to cryopreservation. Core C is far more sophistica...
147 Essays on Infinite Lifespans Brian Wowk 17) Fahy GM & MacFarlane DR & Angell CA & Meryman HT; Vitrification as an approach to cryopreservation in: Cryobiology (1984, Vol. 21); pg. 407 426 18) Rall WF & Fahy GM; Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification in: Nature (1985, Vol. 313); pg. 573 575 19) Song YC & Khirabadi BS & Lightfoot F & Brockbank KG & Taylor MJ; Vitreous cryopreservation maintains the function of vascular grafts in: Nature Biotechnology (2000, Vol. 18); pg. 296 299 20) Fahy GM & Wowk B & Wu J & Paynter S; Improved vitrification solutions based on the predictability of vitrifica- tion solution toxicity in: Cryobiology (in press) 21) Wowk B & Leitl E & Rasch CM & Mesbah-Karimi N & Harris SB & Fahy GM; Vitrification enhancement by synthetic ice blocking agents in: Cryobiology (2000, Vol. 40); pg. 228 236 22) Wowk B & Fahy GM; Inhibition of bacterial ice nucle- ation by polyglycerol polymers in: Cryobiology (2002, Vol. 44); pg. 1423 23) Fahy GM ...
We offer a highly reasonable cost for cryopreservation. Our low-cost is the reason that patients from over the world choose India for receiving the cryopreservation treatment
mouse embryos Vitrification Freezing of mouse spermatozoa ICSI Freezing of oocytes Freezing of ovaries Gnotobiology Health monitoring Links Internal Site Bibliography Freezing of mouse spermatozoa Key references Landel CP Archiving mouse strains by cryopreservation Lab Anim NY 2005 34 50 7 PMID 15806091 Marschall S A Boersma and M H de Angelis 2009 Sperm cryopreservation and in vitro fertilization Methods Mol Biol 530 407 420 PMID 19266334 Marschall S Huffstadt U Balling R Hrabe de Angelis M Reliable recovery of inbred mouse lines using cryopreserved spermatozoa Mamm Genome 1999 10 773 6 PMID 10430662 Nakagata N Cryopreservation of mouse spermatozoa Mamm Genome 2000 11 572 6 PMID 10886025 Ogonuki N K Mochida H Miki K Inoue M Fray T Iwaki K Moriwaki Y Obata K Morozumi R Yanagimachi and A Ogura 2006 Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring Proc Natl Acad Sci U S A 103 13098 13103 PMID 16920794 Ostermeier ...
Tobacco BY-2 suspension cells were successfully cryopreserved by a vitrification method combined with an encapsulation technique. Cell cultures cryopreserved using the optimal conditions established in this study could be thawed and grown enough to subculture in fresh medium within 14 days. However, the vitrification method was less effective for cryopreservation of BY-2 than a simplified slow prefreezing method.. ...
The Cantabrian brown bear survives as a small remnant population in northern Spain and semen cryopreservation for future artificial insemination is one of the measures being implemented for conservation of this species. As part of this program we investigated the value of adding heat shock protein A8 (HSPA8) to media (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid-TRIS-fructose with 20% egg yolk) used for chilling and cryopreserving the spermatozoa. Semen samples from eight brown bears were obtained by electroejaculation during the breeding season. In experiment 1, we tested three concentrations of HSPA8 (0.5, 1, and 5 μg/mL) to determine whether sperm motility (computer assisted sperm analysis system) and sperm survival could be improved during refrigeration (5 °C) up to 48 hours. Results showed that sperm viability (test with propidium iodide) was improved by the addition of 0.5 and 5 μg/mL HSPA8. In experiment 2, HSPA8 was added to the cryopreservation media (6% final glycerol ...
To provide a novel fertility preservation option for patients facing a fertility threatening cancer diagnosis or treatment regimen by establishing an ovarian tissue cryopreservation program. To determine if ovarian tissue cryopreservation provides women with a useful, successful option for fertility preservation. The hypothesis is that ovarian tissue cryopreservation for fertility preservation provides an alternative option for fertility preservation. ...
Whenever a sudden shift is made from fresh embryo transfer to frozen embryo transfer the couple usually have many queries in mind. So, lets evaluate both and see pros and cons.. Fresh embryos transfer is undergoing ovarian hyper stimulation making of embryos and transferring resultant embryos to uterine cavity in same cycle. In frozen embryo transfer first two steps of controlled ovarian hyper stimulation and process of IVF/ICSI are same but the resultant embryos are frozen using vitrification techniques and not transferred in same cycle. They are thawed and then kept uterine cavity after preparing the uterine cavity. In patients who are normal or hyper responders the frozen embryo transfers are known to have better results than the fresh embryo transfer . The reason can be that due to ovarian hyper stimulation and high levels of estrogen may have negative impacts on the endometrial receptivity. In some cycles another hormone called progesterone may also be raised, which again decreases embryo ...
TY - JOUR. T1 - The bioenergetics of mitochondria after cryopreservation. AU - Fuller, Barry J.. AU - Rubinacci, Alessandro. AU - Geboes, Karel. AU - De Loecker, William. PY - 1989. Y1 - 1989. N2 - The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me2SO effected some changes in mitochondrial function.. AB - The functional characteristics ...
Sometimes couples undergoing in vitro fertilization (IVF) need more than a single cycle to conceive. In recent years, frozen embryo transfers (FET) has become a popular option before moving to a fresh IVF cycle. FET at PRC allows you to extend the chance of pregnancy per egg retrieval, saving you time and money.. FROZEN EMBRYO TRANSFER (FET). A frozen embryo transfer (FET) is a cycle in which the frozen embryos from a previous fresh IVF or donor egg cycle are thawed and then transferred back into the womans uterus.. Lower Cost. The cost of frozen egg transfer is significantly lesser than a fresh cycle of IVF. This combination of reduced cost and equal success rates makes frozen embryo transfers an exciting option over fresh IVF cycle at the top IVF & egg fertility clinic in Los Angeles.. Less Medication. In a fresh cycle, you would again need to take stimulation medication. On the other hand FET IVF & egg fertility clinic in Los Angeles just requires you to use estrogen and progesterone to ...
A cohort of 91 children from cryopreserved embryos and 83 control children who were conceived normally had their development assessed using the Griffithss scales of mental development. The controls (81 singletons and two twins) of a similar age, sex, and social class were selected from siblings, cousins, and peers of the cryopreserved embryo group (68 singleton, 20 twins, and three triplets). Children from cryopreserved embryos had a lower mean birth weight and mean gestational age and a higher proportion were born by caesarean section. One child from the cryopreserved embryo group had Downs syndrome, three had squints, and four had conductive hearing loss while in the control children, six had squints, and nine had conductive hearing loss. In both groups, including the child with Downs syndrome, the mean Griffithss quotient was greater than the standard 100. In the children from cryopreserved embryos, the singleton and multiple birth subgroups had statistically similar assessment results. ...
Both proceedures have seen great success rates in the last five years and thousands of baby boys and girls have been born using this technology.. Endocrine Abstracts says, Semen cryopreservation is a viable fertility preservation option for adolescent cancer patients, and should be offered to all before treatment, acknowledging the caveat of a ~50% chance of success. Pubertal staging is the only significant prognosticator of this and should be routinely assessed as part of the counselling process.. While the ethics surrounding fertility preservation in younger cancer patients are being debated, research is ongoing to take fertility tissue from patients where eggs and sperm are not necessarily matured or viable.. Speak to your health professional about fertility preservation options they may be aware of. The two of you will be able to decide if the process is right for you and decide on your next steps.. If youre wary of fertility options, remember, Geoff has managed to father two beautiful ...
There is a lump sum due at the time of cryopreservation. This pays for the cost of transportation, cryopreservation protocols, and also for ongoing costs of maintenance for the cryonics patient. This lump sum is normally covered with a dedicated life insurance policy.. Cryonics organizations also have membership dues, which are separate from the cost of the cryonics life insurance. There are no additional costs due once a member is in cryopreservation.. ...
The summer flounder, Paralichthys dentatus L., is a high-value species and considerable research has been conducted to determine practices conducive for its culture. As milt can be limited in this species, experiments were conducted to develop a practical sperm cryopreservation protocol for hatchery use. Two dilution ratios (1:2 and 1:4; sperm:extender), 2 diluents (saline and sucrose-based), 2 cryoprotectants (10% DMSO and 12% glycerol) and 3 freezing rates (?5, ?10 and ?15°C min?1) were evaluated using differential staining to assess post-thaw sperm survival. Seven combinations of the factors examined reduced post-thaw viability by less than 30%. The average viability of sperm from fresh, pooled flounder milt (67.2 ± 2.9%) was not different from that of thawed milt diluted 1:4 with sucrose diluent (10% DMSO) frozen at ?5°C min?1 (38.4 ± 7.7%) and fertilization and hatch success were not different in trials using fresh or thawed, cryopreserved sperm. From these experiments a practical
Despite of important advances in the knowledge of the reproductive biology of several deer species (mainly, red deer), there is a lot to investigate. We are trying to answer a practical and a basic question: how can we improve sperm cryopreservation in cervids? and, which underlying, cellular and molecular, changes occur during cooling and cryopreservation?. These are important questions not only for our Iberian deer species (Iberian red deer and roe deer), but also to endangered cervids elsewhere in the world (specially South-American species such as the huemul and pudu). Technology transfer from well-known species to related but less studied species might improve their conservation.. ...
IVF and ICSI are fully established methods of assisted reproduction which can be used for patients awaiting cytotoxic therapy:. Following the data of the German, Swiss and Austrian network Fertiprotekt (www.fertiprotekt.eu), 164 of 1388 counselled patients have chosen ovarian stimulation and cryopreservation of oocytes as a fertility preservation technique in 2007 2009. Among those patients 2417 oocytes were collected (Mean: 11.8, Range 0 41, STD 7.3). In 125 patients oocytes were fertilized and cryopreserved, resulting in an fertilisation rate of 70.5%/aspirated oocyte. Only in one patient, chemotherapy needed to be postponed due to severe ovarian hyperstimulation syndrome.. These data reveal that ovarian stimulation can result in adequate numbers of oocytes. However, they also demonstrate, that this technique is not successful in all patients. Combination with cryopreservation of ovarian tissue should therefore be considered. FertiPROTEKT performed a study in which 50% of one ovary was removed ...
Poster (2015, June 14). Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results ... [more ▼]. Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results obtained with HGL5 granulosa cell line suggest that Z-VAD-FMK is efficient to protect granulosa cells from etoposide or CoCl2 induced apoptosis. What is known already: Removal, cryopreservation and subsequent graft of ovarian strips after cancer treatment have been successfully used to re-establish female fertility. However, the pregnancy rate after autografting of cryopreserved tissue is about 30%. Indeed, the major problem after transplantation is follicular loss due to ischemic reperfusion injury. Study design, size, duration: Three human granulosa cell lines (GC1a, ...
TY - JOUR. T1 - Sucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservation. AU - Coticchio, G.. AU - De Santis, L.. AU - Rossi, G.. AU - Borini, A.. AU - Albertini, D.. AU - Scaravelli, G.. AU - Alecci, C.. AU - Bianchi, V.. AU - Nottola, S.. AU - Cecconi, S.. PY - 2006/7. Y1 - 2006/7. N2 - Background: Recently described slow-cooling cryopreservation protocols involving elevated sucrose concentration have improved survival frequencies of human oocytes, potentially overcoming a major hurdle that has limited the adoption of oocyte storage. Because implantation rates of embryos from frozen oocytes remain generally low, it is still debated whether, irrespective of survival rates, this form of cryopreservation leads inevitably to the disruption or complete loss of the metaphase II (MII) spindle. Methods: Human oocytes with an extruded polar body I (PBI) were cryopreserved using a slow-cooling method including 1.5 ...
This three-days training course is specifically designed for Embryologists interested in acquiring practical skills and updating their knowledge on oocyte, embryo, and blastocyst cryopreservation techniques. The course will focus on didactic and intensive hands-on practical lessons on the vitrification system and media that the embryologist needs to implement, pointing out the tips and tricks to achieve consistent results. Throughout the three-days, embryologists will have the opportunity to vitrify and warm unlimited number of oocytes and embryos needed to understand how important little details can affect results. The course also covers the fundamental principles of the assisted hatching procedure and its multiple applications in different developmental stages. Introduction to the Laser Assisted Hatching and familiarization with different operational modes. Evaluation and discussion about the effect of the size of the hole on the developing embryo by culturing treated embryos in a TL ...
By Leigh E. Towill (auth.), Dr. Leigh E. Towill, Prof. Dr. Y. P. S. Bajaj (eds.). Ex situ upkeep of germplasm for better plant species has been accom- plished utilizing both seeds or clones, yet garage of those less than commonplace condi- tions doesnt give you the severe longevities which are had to reduce danger of loss. expenditures of upkeep and regeneration of shares also are excessive. platforms that supply almost indefinite garage should still complement current tools and its inside this context that cryopreservation is gifted. using low temperature upkeep was once firstly extra a priority of drugs and animal breeding, and used to be multiplied to crops within the Nineteen Seventies. Sur- vival after cryogenic publicity has now been validated for various plant teams together with algae, bryophytes, fungi and better vegetation. If survival is com- monplace, then the eventual program is a cryopreservation process, wherein cells, tissues and organs are held indefinitely to be used, usually ...
The effect of cryopreservation and long-term liquid nitrogen storage on peripheral blood mononuclear cell (PBMC) subsets was prospectively analyzed using monoclonal antibodies and flow cytometry. Brief cryopreservation did not significantly alter the proportion of positively stained cells for CD3+, CD4+, CD8+, CD14+, CD16+, and CD19+ cells. A small but statistically
Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to Show moreCryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to assess the ...
Frozen embryo transfers have higher success rates than fresh embryo transfers. Within frozen embryo transfers there are natural and programmed cycles. Each have tradeoffs in terms of cost, intensity, drug requirements and more.
Results of this study10 were surprising, to say the least. Without going into the specifics of different types of fertility preservation and their respective indications, less than half of the respondents (45.6%) reported being aware of fertility preservation. Specialists in O&G fared no better in this regard with only half (50.7%) of the respondents reporting themselves as being aware. As expected, O&G specialists were more aware of fertility-preservation techniques in females such as oocyte- and embryo-freezing as well as ovarian tissue freezing, than their non-O&G counterparts. Interestingly, when respondents were further asked about individual fertility-preservation procedures, an increased awareness was found. In fact, a higher percentage of the same O&G specialists in this study reported to be familiar with all of the above fertility-preservation techniques previously itemised, compared with being aware of fertility preservation per se (63.6% vs 50.7%). These findings highlight a ...
Ovarian tissue cryopreservation (OTC) is offered to women treated for acute leukemia to preserve their fertility before hematopoietic stem cell transplantation. The risk of leukemic infiltration in ovarian samples harvested before administration of chemotherapy limits ovarian tissue transplantations. We assessed the minimal residual disease (MRD) by sensitive quantitative polymerase chain reaction in cryopreserved ovarian cortex and medulla samples harvested from 30 patients in complete remission of acute leukemia, including 60 % with negative bone marrow MRD at the time of OTC. Ovarian MRD was undetectable in 21 patients (70%), detectable below 10-4 in 8 patients (27%) and between 10-3 and 10-4 in 1 patient (3%). Twenty patients (67%) had concordant MRD between bone marrow and ovarian samples. Interestingly 4 patients had positive MRD in ovarian samples while undetectable in bone marrow. Our results underline the importance of reaching the best control of the disease with undetectable or low ...
Mary Zelinski, PhD finishes her reports from the annual meeting of the Society for Cryobiology held from Corvallis, Oregon with a final blog about the keynote
The purpose of this study was to test of efficacy of melatonin on the Optimizing of cryopreservation media in the testis tissue samples. Testes from neonate BALB/c mice were vitrified and then thawed under standard condition with or without the addition of 100 μM melatonin to both of vitrification and thawing solution. After that, Vitrified-thawed whole testes were digested under standard condition and subsequent viability of the cells in the suspension was analyzed using cytotoxicity kit and Apo-Brdu tunnel assay kit. The mean proportion of apoptotic testicular cells in the treated vitrified-thawed testes in comparison to no-treated ones was noted significantly (5.2±0.47 vs. 1.56±0.62, respectively). Moreover, melatonin cause decreasing the viability of the treated vitrified-thawed testicular cells in compared to no-treated vitrified-thawed testicular cells (4.78±0.46 vs. 8.39±0.76, respectively). In addition, the mean cytotoxicity of melatonin on the vitrified-thawed testicular cells was ...
FERRARI, Edilene Aparecida Preti; COLOMBO, Ronan Carlos; FARIA, Ricardo Tadeu de and TAKANE, Roberto Jun. Criopreservação de sementes de Encholirium spectabile Martius ex Schultes f. pelo método da vitrificação. Rev. Ciênc. Agron. [online]. 2016, vol.47, n.1, pp.172-177. ISSN 0045-6888. http://dx.doi.org/10.5935/1806-6690.20160020.. A bromélia Encholirium spectabile Martius ex Schultes f. é uma espécie endêmica da Caatinga. O objetivo deste trabalho foi avaliar a eficiência de soluções crioprotetoras em sementes de bromélia. Os tratamentos consistiram da imersão das sementes em soluções crioprotetoras e de vitrificação, antes da imersão em nitrogênio líquido (NL) (-196 ºC), conforme os tratamentos a seguir: T1 - controle: sem crioprotetores; T2 - glicerol 2M (20 min) + PVS2 (10 min); T3 - glicerol 2M (20 min) + PVS2 com floroglucinol a 1% (10 min); T4 - sacarose 0,4M (20 min) + PVS2 (10 min); T5 - sacarose 0,4M (20 min) + PVS2 com floroglucinol a 1% (10 min); T6 - glicerol ...
The Fertility Preservation Program also will endeavor to aid women and girls who are starting cancer treatment or preparing for a bone marrow transplant. Currently their choices are quite limited and, for many, biologically impossible or morally unacceptable, noted Joseph S. Sanfilippo, M.D., director of the Center for Fertility and Reproductive Endocrinology at Magee-Womens Hospital.. Women can undergo ovarian stimulation to generate multiple mature eggs, which can then be fertilized with partner or donor sperm to produce embryos for later implantation and possible pregnancy, he said. These techniques are now well-established, and we will offer them to female patients who wish to pursue this option. Another option is ovarian cryopreservation, where an ovary is removed before cancer therapy is initiated with the plan to replace ovarian tissue once the patient is cured.. Still, the stimulation technique takes time and could delay cancer treatment. It also produces high estrogen levels that ...
Tissue banking involves the collection and storage of patient tissue samples for future biomedical research [1]. Banking of human tissue is an important tool for advancing translational research, allowing the study of genes, RNA and proteins to explore the biological mechanisms that underpin disease aetiology and biology, and the development of novel treatments [2]. In cancer, biobanking and the research that follows has capacity to facilitate more personalised therapies, with greater likelihood of benefit and fewer adverse effects. Given that patients who have been diagnosed with cancer may be more likely to experience some degree of benefit from biobanking, and can provide tissues of most interest to translational biomedical research, there is a critical need to understand potential donors willingness to participate in tissue banking, the factors influencing their decisions, and their preferences for how their samples are used.. Studies of the general public suggest a variable willingness to ...
Although semen cryopreservation is widely and commonly used in the bovine breeding industry, half the spermatozoa do not survive and most of those that do survive undergo numerous physiological changes that affect their fertilising ability. The aim of the present study was to determine how cryopreservation affects the intracellular events involved in sperm capacitation and acrosome reaction. Immediately after thawing and washing, almost 50% of spermatozoa were capacitated and more than 20% had lost their acrosome. The sperm cAMP concentration was lower than that in freshly ejaculated spermatozoa, but the cytosolic pH (pHcyt) was in the expected range. The free cytosolic Ca2+ concentration ([Ca2+]cyt) was higher than in fresh spermatozoa and cryopreserved spermatozoa had internally stored Ca2+. Phenylarsine oxide increased pHcyt and both cytosolic and stored Ca2+ concentrations, whereas orthovanadate enhanced acrosome loss and protein tyrosine phosphorylation (P-Tyr). Heparin increased the ...
Factors such as gender, education and insurance status may impact whether patients take actions to preserve fertility during cancer treatment.
Thermal exposure indicators, cryopreservation, IVF, egg bank, frozen eggs, frozen sperm, cord blood, oocytes, embryos, bone marrow, vitrified specimens, stem cells, frozen blood plasma, bone banks, bone grafts, tissue banks, sperm cells, cold chain management, frozen vaccines, frozen pharmaceuticals, clinical specimens, premixed adhesives, heat sensitive materials
TY - JOUR. T1 - Cryopreservation of adenovirus-transfected dendritic cells (DCs) for clinical use. AU - Gülen, D.. AU - Maas, S.. AU - Julius, H.. AU - Warkentin, P.. AU - Britton, H.. AU - Younos, I.. AU - Senesac, J.. AU - Pirruccello, Samuel M.. AU - Talmadge, J. E.. PY - 2012/5. Y1 - 2012/5. N2 - In this study, we examined the effects of cryoprotectant, freezing and thawing, and adenovirus (Adv) transduction on the viability, transgene expression, phenotype, and function of human dendritic cells (DCs). DCs were differentiated from cultured peripheral blood (PB) monocytes following Elutra isolation using granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) for 6 days and then transduced using an Adv vector with an IL-12 transgene. Fresh, cryopreserved, and thawed transduced immature DCs were examined for their: 1) cellular concentration and viability; 2) antigenicity using an allogeneic mixed lymphocyte reaction (MLR); 3) phenotype (HLA-DR and CD11c) and ...
Induct yourself into a one-on-one training to develop knowledge and hands-on skills in all aspects of Cryotech vitrification of human oocytes and embryos. Key expert tips & tricks to achieve 100% survival gained will be imparted. At the end of the course, the candidates will be confident to achieve near 100% survival for eggs and embryos in their laboratories.. Course Objectives. During the course each participant receives one-on-one training to :. ...
Pre-Mortem Cryopreservation: Recognizing a Patients Right to Die in Order to Live Ryan Sullivan* I. INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 II. THE SCIENCE OF CRYONIC PRESERVATION . . . . . . . . . . . . 53 A. History of Cryonics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 B. The Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 C. Current Science . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 1. Cryobiology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 a. Successful Births with Once-Frozen Embryos. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 b. If You Can Thaw a Frozen Dog . . . . . . . 63 c. Surgeries Performed During Suspended Animation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 d. Successful Revival After Three Hours of Clinical Death . . . . . . . . . . . . . . . . . . . . . . . . . . 65 2. Advancements in ...
Hematopoietic stem cell cryopreservation significantly contributed to autologous stem cell transplantation (ASCT). Cryopreserved stem cell units (SCU) are expected to be used soon after harvesting for most purposes, but, in a number of cases, they remain stored for some time, creating an increasing load for SCU depositories. Disposal policies vary widely in each center, and the existing guidelines are insufficient. METHODS: We conducted a survey of seven Gruppo Italiano Trapianto di Midollo Osseo centers to investigate the outcome of SCU harvested from January 2005 to December 2009 for ASCT. The data from 1603 collections were gathered, for a total of 5822 SCU. RESULTS: In our cohort, 79% of patients collected ,5 × 10⁶ CD34+ cells/kg, and 3.4% collected ,2 × 10⁶ CD34+ cells/kg. Up to 21% of all the patients and 42% of those with acute leukemia did not undergo reinfusion, and 37% of the cryopreserved SCU were excess, resulting from patients not reinfusing or partially reinfusing. Less than ...
For decades, fertility clinics utilized a slow freeze technique for freezing of eggs and embryos. This method was successful with embryos, but frozen eggs showed only a 50% survival rate. It was clear that a more efficient and successful technique was needed. Today, vitrification allows for 90% or greater survival for both embryos and eggs.
Microbial communities enriched from diverse environments have shown considerable promise for the targeted discovery of microorganisms and enzymes for bioconversion of lignocellulose to liquid fuels. While preservation of microbial communities is important for commercialization and research, few studies have examined storage conditions ideal for preservation. The goal of this study was to evaluate the impact of preservation method on composition of microbial communities enriched on switchgrass before and after storage. The enrichments were completed in a high-solid and aerobic environment at 55 °C. Community composition was examined for each enrichment to determine when a stable community was achieved. Preservation methods included cryopreservation with the cryoprotective agents DMSO and glycerol, and cryopreservation without cryoprotective agents. Revived communities were examined for their ability to decompose switchgrass under high-solid and thermophilic conditions. High-throughput 16S rRNA ...
As many of you may know, when Andrew and I began the process of IVF, we did not anticipate doing a frozen embryo transfer. Our plan was to have the egg retrieval, and then have a fresh transfer 3-5 days later. Due to complications (OHSS), we were not able to have a fresh transfer, and had to wait several weeks before
Any cell that has ever survived freezing or vitrification has recovered from imperfect preservation. Cells cooled below -100ºC enter an alien state in which most cell water is replaced by solutes, molecules deform from normal shapes, and even cell membranes undergo phase transitions. After warming and removal of cryoprotectant, cells engage in considerable self-repair before operating normally again.. It is a premise of cryonics that natural self-repair is not all that will ever exist in medicine. And indeed, it already is not, since molecular intervention in cell death following cryopreservation has already begun in mainstream cryobiology. Cryonicists have been envisioning cell repair augmentation by drugs, synthetic enzymes, viruses, and macrophages since the 1960s. These ideas, part of a biological tradition of diffusion-driven chemistry, are now termed wet nanotechnology. In the 1980s, a new type of nanotechnology based on positional control of chemical reactions was proposed in a ...
PURPOSE Cryopreservation of testicular tissue with subsequent reimplantation after therapy has the potential to preserve fertility for prepubertal boys with cancer. We present the histology and feasibility of testicular tissue procurement for this novel approach. MATERIALS AND METHODS We performed a prospective cohort study of boys at significant risk for treatment associated gonadotoxicity who were eligible for an experimental research protocol between 2008 and 2011. Open testicular biopsy was performed while the patients were anesthetized for another treatment related procedure. Half of the specimen was reserved for cryopreservation, while the other half was used for research purposes. Semithin sections of the biopsy specimens were evaluated for histological features and compared to age adjusted reference values. RESULTS A total of 34 boys underwent biopsy between March 2008 and October 2011. Of the patients 29 had solid tumors and 5 underwent hematopoietic stem cell transplantation for benign
What should we use as medium for cryopreservation of human esophageal cancer cell line - FBS with 10% DMSO OR its own medium with 10% DMSO ...
0028] The pellet-forming receptacle preferably comprises a material that has the characteristics of physical flexibility, pliability, and resistance to breaking or cracking. The material used in forming the surface of the receptacle is preferably hydrophobic, and more preferably allows a resting water droplet to exhibit a contact angle (θc) of greater than about 90°. The surface of the receptacle preferably has low chemical reactivity and thus is substantially non-stick to facilitate complete release of the pellets from the receptacle wells after cryopreservation, as described herein. Advantageously, because the pellets are completely released intact leaving behind no residue, the receptacle can then be reused. The material used to form the surface preferably has a very low coefficient of friction (and preferably less than about 0.5, more preferably less than about 0.1) at temperatures below zero degrees Celsius. More preferably, the material retains one or more of the foregoing ...
The Congress Secretariat and Organizers cannot accept liability for either personal accidents nor loss of or damage to private property of participants, either during or directly arising from The 3rd International Congress on Controversies in Cryopreservation of Reproductive cells, Tissue and Organs (CRYO). Participants should make their own arrangements with respect to health and travel insurance. ...
Citation: Volk, G.M. 2011. Strategies for improved fruit crop cyropreservation [meeting abstract]. In: Proceedings of The First International Symposium on Cyropreservation of Horticultural Crops in China, June 28-30, 2011, Yangling, Shaanxi, China. p 43. Interpretive Summary: Technical Abstract: Fruit crop collections kept in fields or greenhouses are expensive to maintain. Cryopreservation has been implemented as a method to back-up some of these costly collections, but it is a labor-intensive process. My laboratory has been working toward finding practical approaches to decrease the labor inputs and costs of the cryopreservation process. These include ensuring that the appropriate conservation target is used (shoot tips, dormant buds, pollen, seeds) and that the cryopreservation strategy increases the likelihood of survival with less handling. Recently we have identified successful methods for conserving diverse Citrus species using shoot tips derived directly from screenhouse-grown plants. We ...
TY - JOUR. T1 - Beneficial effect of microinjected trehalose on the cryosurvival of human oocytes. AU - Eroglu, Ali. AU - Toner, Mehmet. AU - Toth, Thomas L.. PY - 2002/2/5. Y1 - 2002/2/5. N2 - Objective: To determine the effectiveness of trehalose as an intracellular cryoprotectant for the cryopreservation of human oocytes. Design: In vitro comparative study. Setting: Clinical and academic research environment at a medical school teaching hospital. Patient(s): Women undergoing in vitro fertilization (IVF). Intervention(s): Discarded human oocytes, obtained from IVF patients, were randomly distributed into three groups: control group (no trehalose), extracellular trehalose group (0.5 M extracellular trehalose), and intracellular trehalose group (0.15 M intra- and 0.5 M extracellular trehalose). Trehalose was introduced into oocytes by microinjection. The oocytes in each group were cooled to different temperatures (i.e., -15°C, -30°C, and -60°C) at rate of 1°C/minute and thawed at ambient air ...
Fertility Preservation options for men & women GnRH analog, semen & egg freezing & Ovarian tissue cryo-banking due to cancer, aging, reproduction problems. Call us to make an appointment to preserve your fertility with family preservation treatments. We use the latest therapies to achieve successful results.
Vitrification is a method of cryopreserving tissue for future use. It is widely used to preserve extra, good quality embryos generated from infertility treatments. It is becoming popular for the preservation of oocytes (eggs) as well, but is still considered investigational in this respect. The purpose of this study is to provide egg freezing for patients desiring fertility preservation. Although commonly used in clinics around the world, it should be offered as an IRB approved study procedure until it is no longer considered investigational by the American Society of Reproductive Medicine ...
Fertility Preservation options for men & women GnRH analog, semen & egg freezing & Ovarian tissue cryo-banking due to cancer, aging, reproduction problems.
Cryotop (Kitazato BioPharma Co, Fuji-Shizuoka, Japan) oocyte vitrification was performed at room temperature in a home made solution comprising 15% dimethylsulphoxide (DMSO- D 2438 Sigma Aldrich, Steinheim, Germany), 15% ethylene glycol (EG 10.246-6 Sigma Aldrich) and 0.5 mol/L sucrose (Sigma Aldrich), after a gradual initial equilibration of 15 minutes in a solution comprising 7.5% DMSO and 7.5% EG (Kuwayama et al, 2005, Rienzi et al., 2009). For the ultra-rapid cooling, the Cryotops - containing 1-2 oocytes- were plunged into UV-sterilised LN2 . and closed with their plastic caps. Then, the Cryotops of each patient were enclosed in home made hermetical aluminium cylindric containers (high security goblets). which can contain up to 6 Cryotops each. These goblets had been previously submerged vertically in LN2 in order to avoid the infiltration of LN2 and checked for an inner temperature of -196° C at the end of UV-sterilisation process. The Cryotops were inserted into the high security ...
If we were to compare the progression of cryopreservation technology during recent years, we would find that from 2004 to 2013 over a timeframe of a decade, there has been around a 2.5 times increase in the rate of frozen-thawed embryo transfers (FETs) that were reported to the Society for Assisted Reproductive Technology (SART). While the rate of fresh embryo transfers (ET) that were completed was the same.. ...
Cancer survivors, oncologists, and reproductive specialists discuss exploring fertility preservation options for young adults diagnosed with cancer.
Hello, I have my Frozen embryo transfer planned for this Thursday. I only have one that is of good quality and was frozen at 5 days. Has anyone had success with just one? Feeling a little defeated as this is our last chance. Many thanks
Cryopreservation Oocyte cryopreservation KNBC.com June 15, 2005. Retrieved on August 4, 2008 Los Angeles Times, July 18, 2005. ... Jain, J.K.; Francis, M.M.; Bayrak, A.; Quinn, P.; Paulson, R.J. (2005). "Pregnancy Outcome After Cryopreservation of All ... Jain wrote an important review article on oocyte cryopreservation for the journal Fertility Sterility in 2006, identifying him ... "Oocyte cryopreservation". Fertility and Sterility. 86 (4 Suppl): 1037-46. doi:10.1016/j.fertnstert.2006.07.1478. PMID 17008147 ...
Chian, Ri-Cheng (2010). Fertility Cryopreservation. Cambridge University Press. p. 52. ...
Cryopreservation may be accomplished by freezing, freezing with cryoprotectant to reduce ice damage, or by vitrification to ... In London in 2016, the English High Court ruled in favor of a mother's right to seek cryopreservation of her terminally ill 14- ... Cryopreservation of humans is not reversible with present technology; cryonicists hope that medical advances will someday allow ... In the initial cryopreservation protocol, the subject is intubated and mechanically ventilated, and a highly efficient ...
He was also Head of the Tissue Cryopreservation Section of the Transfusion and Cryopreservation Research Program of the U.S. ... More recently, he received the Cryopreservation Award from the International Longevity and Cryopreservation Summit held in ... Fahy has over thirty years of experience in the field of cryopreservation. As a scientist with the American Red Cross, he was ... Life extension Fahy GM, Wowk B, Wu J, Phan J, Rasch C, Chang A, Zendejas E (2004). "Cryopreservation of organs by vitrification ...
Cryobiology Cryopreservation Biobank Tissue Engineering Organ Transplantation Michael J. Taylor; Ying C. Song; Kelvin G.M. ... Gregory M. Fahy, who pioneered the use of vitrification in reproductive cryopreservation, serves on the company's Board of ... Rall WF, Fahy GM (1985). "Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification". Nature. 313 (6003): ... ISBN 0-85200-418-4. Fahy GM, MacFarlane DR, Angell CA, Meryman HT (1984). "Vitrification as an approach to cryopreservation". ...
"Principles of Cryopreservation". Cryopreservation and Freeze-Drying Protocols, Second Edition. Methods in Molecular Biology. ...
It has been explored as an alternative technique for cryopreservation. The technique has the advantages of being able to ...
CS1 maint: Multiple names: authors list (link) Abazari A1, Jomha NM, Elliott JA, McGann LE (2013). "Cryopreservation of ...
It is also commonly used in cryopreservation of blood vessels along with the other glycosaminoglycans and protein suspensions. ... Müller-Schweinitzer E, Hasse J, Swoboda L (1993). "Cryopreservation of human bronchi". J Asthma. 30 (6): 451-7. doi:10.3109/ ... Müller-Schweinitzer E, Ellis P (May 1992). "Sucrose promotes the functional activity of blood vessels after cryopreservation in ... Brockbank KG (February 1994). "Effects of cryopreservation upon vein function in vivo". Cryobiology. 31 (1): 71-81. doi:10.1006 ...
This work is focused on improving the process of cryopreservation itself, e.g., to improve vitrification technology, or to ... Almost as many as 200 Russian citizens have entered into cryopreservation agreements with the company. It is thought that ... Prior to the companys' founding, its prospective staff members have already had some experience in cryopreservation, when in ... This is being justified as follows: "Activities, directed towards cryopreservation of people can be considered fraud if an ...
Henkelman S, Lagerberg JW, Graaff R, Rakhorst G, Van Oeveren W (2010). "The effects of cryopreservation on red blood cell ... Viability assays are used to assess the success of cryopreservation techniques, the toxicity of substances, or the ... Pichugin Y, Fahy GM, Morin R (2006). "Cryopreservation of rat hippocampal slices by vitrification" (PDF). Cryobiology. 52 (2): ... Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...
Prasath, Ethiraj B. (2008-01-01). "Ovarian tissue cryopreservation: An update". Journal of Human Reproductive Sciences. 1 (2): ... Silber, S. J. (2012-02-01). "Ovary cryopreservation and transplantation for fertility preservation". Molecular Human ... and he did important translational research on ovarian tissue cryopreservation and on ovary transplantation. Gosden was born on ... and he did important translational research on ovarian tissue cryopreservation and on ovary transplantation; his interests also ...
"Principles of Cryopreservation by Vitrification". Methods in Molecular Biology. Methods in Molecular Biology. 1257: 30-33. doi: ... "Basic principles of cryopreservation" (PDF). fao.org. "Cryogenic Storage of Human Hematopoietic Progenitor Cells" (PDF). ...
Fahy, Gregory M.; Wowk, Brian (2015). "Principles of Cryopreservation by Vitrification". 1257: 30-33. doi:10.1007/978-1-4939- ... "Cryopreservation of the Brain: An Update" (PDF). Cryonics. http://www.alcor.org/FAQs/faq02.html Fryer, Jane (2006-07-29). "The ...
Porcu, Eleonora; Ciotti, Patrizia; Venturoli, Stefano (2012-12-06). Handbook of Human Oocyte Cryopreservation. Cambridge ... useful for applications such as sperm cryopreservation. The semi-solid mixture can also be called slush nitrogen or SN2. Solid ... in liquid and slush nitrogen by numerical simulation of cooling rates for French straws used for sperm cryopreservation". ...
2005) Biology of Plants, 7th ed., page 459 Reed, Barbara (2008). Plant cryopreservation a practical guide. New York: Springer. ...
The use of cryopreservation agents is also key to the freezing process. A common cryoprotection agent used is 10% solution of ... Coopman, K (2013). Cryopreservation: Technologies, Applications and Risks/Outcomes (PDF). Nova Science Publishers. pp. 91-108. ... Rapid thaws are recommend in bringing the cells out of cryopreservation and starting up their normal metabolic processes. ... Cell banks are commonly used within fields including stem cell research and pharmaceuticals, with cryopreservation being the ...
Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...
"UK Cryonics and Cryopreservation Research Network". Retrieved 2016-02-02. Leonard, Tom (9 June 2013). "Three senior Oxford ... by signing an open letter to support research into cryonics and by being an advisor to the UK Cryonics and Cryopreservation ...
He has also advocated human cryopreservation, for example by signing an open letter to support research into cryonics, being a ... "UK Cryonics and Cryopreservation Research Network". Retrieved 2016-02-02. Benford, Gregory (1992-08-01) [1980]. Timescape. ... member of Alcor, and by being an advisor to a UK cryonics and cryopreservation advocacy group. Gregory Benford became Emeritus ...
Donor blood cryo-preservation also reduces the number of times a donor needs to have blood drawn for cross matching, making ... The implementation of the cryo-preservation service allowed NKR centers to dramatically reduce the time to complete a cross ... doi:10.1111/j.1600-6143.2012.04070.x. "National Kidney Registry Initiates Donor Blood Cryo-Preservation" (Press release). ... Berz; McCormack; Winer; Colvin; Quesenberry (12 Nov 2007). "Cryopreservation of Hematopoietic Stem Cells" (PDF). Am. J. Hematol ...
"Electric and magnetic fields in cryopreservation". Cryobiology. 64 (3): 301-3; author reply 304-5. doi:10.1016/j.cryobiol. ...
Storage by cryopreservation, on the other hand, will be in the −80 to −196 °C temperature range. Organs, and tissues are more ... Cryopreservation of cells is guided by the "two-factor hypothesis" of American cryobiologist Peter Mazur, which states that ... Cryopreservation in humans with regards to infertility involves preservation of embryos, sperm, or oocytes via freezing. ... This led to a much wider use of cryopreservation today, with many organs, tissues and cells routinely stored at low ...
Cryopreservation and culture tissue after cryopreservation. Cryopreservation of ovarian tissue is of interest to women who want ... Oktay K, Oktem O (November 2008). "Ovarian cryopreservation and transplantation for fertility preservation for medical ...
... s are found in some science fiction films such as Prometheus (2012) and The Host (2013). Cryopreservation Kopenkoskey, ...
9 February - A breakthrough in cryopreservation is announced, with a rabbit's whole brain shown to have a well-preserved ... "New cryopreservation procedure wins Brain Preservation Prize". KurzweilAI. 9 February 2016. Retrieved 11 February 2016. " ...
Antifreeze protein Cryobiology Cryopreservation Thorsen, Stig Morten; Höglind, Mats (2010-12-15). "Modelling cold hardening and ...
Pichugin; Fahy; Morin (April 2006). "Cryopreservation of rat hippocampal slices by vitrification". Cryobiology. 52 (2): 228-240 ... Ben thus helped to ensure the completion of the Hippocampal Slice Cryopreservation Project (HSCP), which had begun in 1998, as ... mainly as a result of the discontinuation of service in 1999 by its cryopreservation provider (Biopreservation), he helped ... Interview of Ben Best Cryonics Institute CryoCare Foundation Cryonics Society of Canada The Hippocampal Slice Cryopreservation ...
They used cryopreservation of gametes and embryos. Using cryopreservation and recent cloning technologies are considerations ...
Sperm, eggs and embryos are stored in liquid nitrogen using cryopreservation (defined as the freezing of cells or whole tissues ... Includes safety procedure regulations at fertility clinics; includes safe cryopreservation of eggs and embryos. Eggs and ... A cryoprotective compound (a liquid called cryopreservation medium), along with carefully controlled cooling and warming cycles ...
JAX cryopreservation followed by high success cryorecovery rates makes your strains available for project reinitiation or ... Mouse Cryopreservation, Recovery and Strain Submission. JAX is the global leader in cryopreservation and recovery. Harness our ... Mouse Cryopreservation. Protect your valuable strains from unfortunate events. JAX Cryopreservation Services offer fast and ... or natural disasters with JAX Cryopreservation and Recovery Services. ...
The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for ... Embryo Cryopreservation The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and ... The Process of Cryopreservation. At the Emory Reproductive Center, the mechanical process of embryo cryopreservation is ... Advantages of Cryopreservation. The Ethics Committee of the American Society of Reproductive Medicine has suggested the ...
... Assessment - Thermo Fisher Scientific, Charter Medicals, praxair ... Cryopreservation Equipment Market Stoking Growth by 2025 Cryopreservation Equipment Market: Snapshot Cryopreservation equipment ... Cryopreservation Equipment Market Key Opportunities Prospects 2025 Cryopreservation Equipment Market: Snapshot Cryopreservation ... Cryopreservation equipment include drystore freezers, mechanical freezers, cryopreservation freezers, cryopreservation vials, ...
Patent Eligibility of Cryopreservation Technique Considered by Federal Circuit Blog FDA Life McGuireWoods LLP ... 7,604,929, which is directed to a method of cryopreservation of hepatocytes (liver cells) was invalid under 35 U.S.C. § 101 ... The claims at issue are generally directed to a method of cryopreservation of hepatocytes that would enable the hepatocytes to ... The prior art generally taught that, once cryopreservation was performed once on hepatocytes, the cells would not remain viable ...
28,000 funding needed for cryopreservation of ALS patient Aaron Winborn at Cryonics Institute, according ... HomeNewsFunding for ALS patient Aaron Winborns cryopreservation meets goal Funding for ALS patient Aaron Winborns ... The Society For Venturism has received the remaining $28,000 funding needed for cryopreservation of ALS patient Aaron Winborn ... Cryonics is an experimental cryopreservation procedure using cryoprotectants and temperatures so cold that a person beyond help ...
ForensisGroup will connect you with a highly qualified cryopreservation expert who best fits the specific requirements of your ... Cryopreservation may be used for various purposes, making it all the more important that the right cryopreservation expert is ... Are you looking for a reputable and highly skilled cryopreservation expert witness? Cryopreservation refers to the process of ... Cryopreservation Application Development Immunology Artificial Embryos Biology Cattle Cow Cytology Fertility Infertility In ...
Conclusions/Significance Changes in the sperm head during cryopreservation process have resulted useful to identify the ability ... Background Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the ... Findings We have evaluated the changes that occur in the sperm head dimensions throughout the cryopreservation process. We have ... Sperm Cell Population Dynamics in Ram Semen during the Cryopreservation Process. Table 1. Head morphomertic characteristics of ...
... or requiring fertility preservation prior to undergoing treatment now have an option with the launch of oocyte cryopreservation ... Fertility Specialists of Texas Offers Comprehensive Care Addressing Oocyte Cryopreservation. 2011 ARCHIVAL NEWS CONTENT. PRESS ... Human egg freezing or oocyte cryopreservation is a novel technique intended to preserve a womans eggs for later use in life. ... Fertility Specialists of Texas Offers Comprehensive Care Addressing Oocyte Cryopreservation. Source: Fertility Specialists of ...
Cryopreservation of tobacco BY-2 suspension cell cultures by vitrification with encapsulation * * Kobayashi Toshihiro KOBAYASHI ... Cryopreservation of axillary shoot tips of in vitro-grown grape (vitis) by a two-step vitrification protocol MATSUMOTO T ... Cryopreservation of in vitro-grown axially shoot tips meristems of mint (Mentha spicata L.) by encapsulation vitrification ... Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var Brasiliensis Tanaka) by vitrification SAKAI A. ...
Cryopreservation michelle 2018-01-19T14:16:14+00:00 CRYOPRESERVATION. A full range of cool products ... Vitrification is now the preferred choice for cryopreservation of human gametes and embryos. ...
Cryopreservation is preserving supernumerary embryos at sub-zero temperatures for use in subsequent treatment cycles. The long ... Successful embryo cryopreservation is an important step for IVF laboratories to maximize a patients IVF cycle. Irvine ... Irvine Scientifics vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation ... Vitrification is an ultra‐rapid cryopreservation technique that provides excellent survivability by using optimal ...
Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro ... evaluate Jersey IVP survival rates after three cryopreservation procedures. The factorial experimental design for objectives ... Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy ... Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy ...
Oocyte Cryopreservation abroad in India offers info on cost Oocyte Cryopreservation India,Oocyte Cryopreservation IVF Doctors & ... Egg Cryopreservation To Healthy Women, Human Oocyte Cryopreservation, Oocyte Cryopreservation Delhi, Oocyte Cryopreservation ... Oocyte Cryopreservation, Oocyte Cryopreservation India, Oocyte, Egg Freezing, Womans Eggs, Cryopreservation, Biological Clock ... Home , Fertility , Egg Donation , Oocyte Cryopreservation. Overview. Oocyte Cryopreservation in India. Sperm and embryos ( ...
... in embryo cryopreservation Ovarian tissue in ovarian tissue cryopreservation Plant seeds or shoots may be cryopreserved for ... Cryopreservation for embryos is used for embryo storage, e.g., when in vitro fertilization (IVF) has resulted in more embryos ... Cryopreservation of tissue during recent times began with the freezing of fowl sperm, which during 1957 was cryopreserved by a ... Generally, cryopreservation is easier for thin samples and small clumps of individual cells, because these can be cooled more ...
Embryo cryopreservation is useful for leftover embryos after a cycle of in vitro fertilisation, as patients who fail to ... Cryopreservation of embryos is the process of preserving an embryo at sub-zero temperatures, generally at an embryogenesis ... Cryopreservation of Embryos in Ethylene Glycol Freeze Media and Direct Transfer in Crossbred Cattle in the Tropics,9th ... Embryo cryopreservation is generally performed as a component of in vitro fertilization (which generally also includes ovarian ...
... ABSTRACT: In 2013, the American Society for Reproductive Medicine and the Society for Assisted ... Oktay K, Cil AP, Bang H. Efficiency of oocyte cryopreservation: a meta-analysis. Fertil Steril 2006;86:70-80. [PubMed] [Full ... Human oocyte cryopreservation. Hum Reprod Update 2007;13:591-605. [PubMed] [Full Text] ⇦ ... Mature oocyte cryopreservation is a currently available method of fertility preservation in women of reproductive age. Although ...
In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ... Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in ... Cryopreservation Tools for Microbiology ATCC has the tools you need for the successful cryopreservation of your microbial ... Cryopreservation Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires ...
Embryo cryopreservation is an efficient, reliable, and cost-effective means of safeguarding and maintaining stocks of ... One week before the scheduled cryopreservation date, pick up 1 tube of M2 media, 1 tube of PMSG, and 1 tube of HCG from the ... HCG is given 47 hours after PMS at 9 a.m., one day before the scheduled rederivation date (e.g. Thursday for cryopreservation ... Embryo cryopreservation is an efficient, reliable, and cost-effective means of safeguarding and maintaining stocks of ...
"Here, a paper‐based cell cryopreservation method as an alternative to conventional cryopreservation methods is presented. The ... The paper-based cryopreservation platform can be rolled or folded during storage to save space, and can be cut into small ... These findings, which are described in an article ("Paper‐Based Cell Cryopreservation") in the journal Advanced Biosystems, ... "The continuous development of simple and practical cell cryopreservation methods is of great importance to a variety of sectors ...
... Marlon A. Guerrero Department of Surgery, The University of Arizona, 1501 N. Campbell ... M. F. Herrera, C. S. Grant, J. A. Van Heerden, D. Jacobsen, A. Weaver, and L. A. Fitzpatrick, "The effect of cryopreservation ... C. R. McHenry, D. B. Stenger, and N. K. Calandro, "The effect of cryopreservation on parathyroid cell viability and function," ... M. F. Brennan, E. M. Brown, H. F. Sears, and G. D. Aurbach, "Human parathyroid cryopreservation: in vitro testing of function ...
... Service. The JAX Sperm Cryopreservation method was developed by our reproductive science experts and has ... Sperm Cryopreservation techniques were developed by JAX scientists and are an essential tool for efficient colony management. ...
... while also reviewing the history of cryopreservation. Its most unique feature is the breadth of its coverage, from basic ... information on reproduction in fishes, to such advanced topics as embryo cryopreservation. ... This edited book addresses central issues in fish gamete cryopreservation and breeding, ... along with detailed information on embryo cryopreservation in fishes and crustaceans. The role of cryopreservation in ...
Cryopreservation is the branch of science in which the effects of very low temperature on living beings at cellular level are ... Massip A (2001) Cryopreservation of embryos of farm animals. Reprod Domes Anim 36(2):49-55CrossRefGoogle Scholar ... Rall WF, Fahy GM (1985) Ice-free cryopreservation of mouse embryos at −196°C by vitrification. Nature 313:573-575CrossRefPubMed ... Friedler S, Giudice LC, Lamb EJ (1988) Cryopreservation of embryos and ova. Fertil Steril 49(5):743-764CrossRefPubMedGoogle ...
P. C. Stanwood, "Cryopreservation of seed germplasm for genetic conservation," in Cryopreservation of Plant Cells and Organs, K ... P. Chmielarz, "Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra," Acta Biologica Hungarica, vol. 61, no. 2, ... V. C. Pence, "Cryopreservation of seeds of Ohio native plants and related species," Seed Science and Technology, vol. 19, no. 2 ... F. Engelmann, "Plant cryopreservation: progress and prospects," In Vitro Cellular and Developmental Biology-Plant, vol. 40, no ...
... by R. Michael Perry. [Update of the article Options for Brain-Threatening Disorders ... Resuscitation from cryopreservation is a subject that has many divergent points of view even among those who accept the basic ... Though cryonicists see cryopreservation as a medical procedure, legally it qualifies as "disposal of a dead body" (or other ... Better still would be to have cryopreservation treated as a medical procedure which could be freely chosen and started at any ...
... by Aschwin de Wolf The goal of human cryopreservation standby and ... The choice of diprivan is a typical example of the sort of trade off that sometimes needs to be made in human cryopreservation ... In human cryopreservation vasopressors (pressors) are used to increase blood pressure and selectively shift blood flow to the ... Because avoiding some of the side effects of these medications is not as high a priority in human cryopreservation as in ...
Download this Cryopreservation photo now. And search more of iStocks library of royalty-free stock images that features Animal ...
Natures antifreeze inspires revolutionary bacteria cryopreservation technique *Synthetic reproductions of antifreeze proteins ... The Warwick team, led by Professor Matthew I. Gibson, has developed a new method for cryopreservation, inspired by the process ... By combining two polymers to slow ice growth during cryopreservation, the researchers were able to recover more bacteria after ... Natures antifreeze inspires revolutionary bacteria cryopreservation technique * UKs first access course in Islamic Education ...
... which made it practically unsuitable for cryopreservation. With that in mind, the team studied the characteristics of the ... scientists from the Australian Institute of Marine Science and the Taronga Conservation Society in coral reef cryopreservation ...
Home , Cryonics - the cryopreservation of whole bodies , Cryopreservation of tissue. Cryopreservation of tissue. The ... For information on cryopreservation relating to fertility treatment, please refer to the Human Fertilisation and Embryology ... cryopreservation of entire bodies has never been proven to work. However, it is possible to cryopreserve smaller body parts and ...
Effects of cryopreservation on lymphocyte immunophenotype and function.. Costantini A1, Mancini S, Giuliodoro S, Butini L, ... Cryopreservation of isolated peripheral blood mononuclear cells (PBMCs) for phenotypic and functional analyses is considered a ... We thus conclude that cryopreservation induces a consistent set of changes in PBMCs from both healthy and HIV-infected ... However, only few and somewhat conflicting data are presently available on the effects of cryopreservation on PBMCs, especially ...
Researchers report the first evidence of cryopreservation by an overwintering insect in which stores of an uncommon lipid are ... Researchers show that tissues are more likely than single cells to suffer damage during cryopreservation because of the tight ...
NutriFreez® brand of cryopreservation media has set the standard for high-quality freezing solutions. It is the first fully ... NutriFreez® D10 Cryopreservation Medium without Phenol Red Item no.: 05-714-1B ... Powerful cryopreservation media optimized for enhanced performance across a broad range of cells. ... NutriFreez® Media maintains animal component-free conditions during cryopreservation, essential to maintaining consistency when ...
CONCLUSION: The data may help establishing better cryopreservation protocols for perspective chorionic cell lines and their ... OBJECTIVE: To investigate the effect of cryopreservation on proliferation and differentiation potential of chorion cell culture ... CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or ...
Ovarian tissue cryopreservation Survival of tissue undergoing cryopreservation, thawing and maturation of immature eggs. ... Cryopreservation of Ovarian Tissue. The safety and scientific validity of this study is the responsibility of the study sponsor ... Ovarian tissue cryopreservation. Ovarian tissue transplantation. Fertility preservation. Risk of premature ovarian failure. ... CRYOPRESERVATION OF OVARIAN TISSUE FOR POTENTIAL IN VITRO MATURATION OR AUTOLOGOUS TRANSPLANTATION [ Time Frame: 6 months to a ...
Upon death, her body was cryopreserved--frozen--and relocated to a cryopreservation facility called the Alcor Life Extension ...
Current Frontiers in Cryopreservation. Edited by: Igor I. Katkov. ISBN 978-953-51-0302-8, PDF ISBN 978-953-51-4326-0, Published ... Current Frontiers in Cryopreservation. Edited by Igor Katkov. Institute for Medical Research, United States of America ... Marine Fish Sperm Cryopreservation and Quality Evaluation in Sperm Structure and Function. By Qing Hua Liu, Zhi Zhong Xiao, Shi ... Review on Ovarian Cryopreservation in Large Animals and Non-Human Primates. By Milan Milenkovic, Cesar Díaz-Garcia and Mats ...
Maximizing the retention of antigen specific lymphocyte function after cryopreservation.. Disis ML1, dela Rosa C, Goodell V, ... We determined several factors which do not affect cell viability after cryopreservation such as shipping frozen samples on dry ... We evaluated a variety of cryopreservation strategies with the aim of developing an optimized protocol for freezing and thawing ... we used an optimized cryopreservation protocol with different media additives to determine if functional T cell responses to ...
Only droplet-vitrification ensured survival and regrowth after cryopreservation. After cryopreservation, regeneration of apical ... Cryopreservation of Redwood (Sequoia Sempervirens (D. DON.) ENDL.) in Vitro Buds Using Vitrification-Based Techniques ... In this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation- ... With basal buds, regeneration after cryopreservation was possible over a larger range of PVS2 treatment durations, between 30 ...
Cryopreservation. Latest News. Latest developments in the freeze drying of red blood cells using biomimetic apatites. A ...
  • For the third objective, grade 1 Jersey IVP blastocysts (n=356) were divided into six treatments using a 2x3 factorial design comparing intact (IB) vs collapsed blastocoele (CB) and three cryopreservation methods: slow freezing (SF) vs vitrification using open pulled straws (OPS) or cryotop (CT). (calpoly.edu)
  • Vitrification now is considered the most effective method of embryo cryopreservation and performed mostly in all IVF clinics all other the world. (olgafertilityclinic.com)
  • Vitrification and freeze thaw are the two methods of cryopreservation in IVF. (transparencymarketresearch.com)
  • Oocyte cryopreservation, like sperm cryopreservation, presents us with the possible opportunity to preserve one's fertility while avoiding these ethical dilemmas. (indiahospitaltour.com)
  • Sperm cryopreservation is also done in which the sperms are saved and preserved in a lab for future use. (ivfsurrogacy.in)
  • Although both sperm and embryo cryopreservation have become commonplace, oocyte preservation or the freezing of unfertilized oocytes (or eggs) for similar applications in women has not historically delivered the acceptable success rates necessary to drive adoption across the board. (indiahospitaltour.com)
  • In addition, oocytes are more vulnerable ice damage due to microtubule structure, which is likely to support growth of the global cryopreservation for in-vitro fertilization (IVF) market in the near future. (transparencymarketresearch.com)
  • Background Simple and effective cryopreservation of human oocytes would have an enormous impact on the financial and ethical constraints of human assisted reproduction. (niu.edu)
  • Recently, studies have demonstrated the potential for cryopreservation in an ice-free glassy state by equilibrating oocytes with high concentrations of cryoprotectants (CPAs) and rapidly cooling to liquid nitrogen temperatures. (niu.edu)
  • In addition, cryopreservation of embryos and gametes for bio banks and IVF settings has emerged as an alternative option to usual practices for utilizing fresh embryos. (transparencymarketresearch.com)
  • Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). (uantwerpen.be)
  • Conclusions The piecewise-constant procedures described in this study are experimentally facile and are predicted to be less toxic than conventional procedures for human oocyte cryopreservation. (niu.edu)
  • Only high quality embryos are selected for cryopreservation since poor quality embryos seldom survive the freezing and thawing processes. (emoryhealthcare.org)
  • However, not all the extra embryos produced in the procedure are suitable for cryopreservation and only the best quality embryos are selected for the procedure. (ivfsurrogacy.in)
  • While there is an implicit recognition of the fact that different manufacturing protocols may have specific effects on different cell types, cryopreservation protocols are frequently derived from our experience in the cryopreservation of stem cell products and do not account for the heterogeneous functional nature of DLI T-cell populations. (plu.mx)
  • 2. International Symposium on Plant Cryopreservation. (cgiar.org)
  • The main reason for this lack of availability is the lack of an effective cryopreservation protocol. (uantwerpen.be)
  • Here, we report the results of a prospective, multicenter trial on the effect of four different cryopreservation solutions that were used to freeze DLIs compared to control DLIs that were refrigerated overnight. (plu.mx)
  • Cryopreservation and orthotopic transplantation of mouse ovaries: new" by J Sztein, H Sweet et al. (jax.org)
  • Cryopreservation and orthotopic transplantation of mouse ovaries: new approach in gamete banking. (jax.org)
  • The quality of the embryo cryopreservation program at Emory is reflected in the pregnancy rates achieved with frozen embryo transfer. (emoryhealthcare.org)
  • The prior art generally taught that, once cryopreservation was performed once on hepatocytes, the cells would not remain viable for further testing if frozen a second time. (lexology.com)
  • Recently, I've heard of something called Oocyte cryopreservation , where a (fertilized, I think) egg from a woman is extracted, frozen and later thawed and reinserted into the woman to delay pregnancy. (stackexchange.com)
  • CONCLUSION: While aCGH and subsequent frozen embryo transfer are currently used to screen embryos, this is the first investigation to quantify the impact of aCGH specifically on embryo cryopreservation. (ox.ac.uk)
  • Cryopreservation may be used to preserve embryos, eggs, sperm, and ovarian tissue, which may allow for childbirth when it would otherwise not be possible. (forensisgroup.com)
  • In addition to biological tissue found in humans, cryopreservation may also be used for studying fungi, moss, and various forms of bacteria. (forensisgroup.com)
  • The benefit of cryopreservation is that a woman has an additional chance to conceive without new stimulation procedure and follicular puncture in the donor. (olgafertilityclinic.com)
  • Clinical methods of cryopreservation for donor lymphocyte infusions vary in their ability to preserve functional T-cell subpopulations. (plu.mx)
  • Our low-cost is the reason that patients from over the world choose India for receiving the cryopreservation treatment . (ivfsurrogacy.in)
  • Our cryopreservation cost in India is the most reasonable, which is why international patients choose our services. (ivfsurrogacy.in)
  • JAX Cryopreservation Services offer fast and reliable recovery of archived embryos and sperm so you can feel assured about the safety of your strains. (jax.org)
  • Protect your strains from pathogen outbreaks, genetic drift, breeding cessation, or natural disasters with JAX Cryopreservation and Recovery Services. (jax.org)
  • Cryopreservation of surplus embryos is a biological 'support' for the couple since it is not known whether the next possible fertilization attempt will be successful or the same donor will be available. (olgafertilityclinic.com)
  • This investigation evaluated an application for array comparative genomic hybridization (aCGH) in assessment of surplus embryos prior to cryopreservation. (ox.ac.uk)
  • IVF patients should be counseled that the benefits of aCGH screening will likely come at the cost of sharply limiting the number of surplus embryos available for cryopreservation. (ox.ac.uk)
  • After three months of cryopreservation at -80 degrees C, we found that metabolic activity, in terms of the specific activity recovery of MOB, aerobic ammonium oxidizing bacteria (AerAOB) and anaerobic AOB (AnAOB, anammox) in the OLAND mixed culture, resumes sooner when one of our selected CPA [dimethyl sulfoxide (DMSO) and DMSO plus trehalose and tryptic soy broth (DMSO+TT)] was added. (uantwerpen.be)
  • The claims at issue are generally directed to a method of cryopreservation of hepatocytes that would enable the hepatocytes to withstand multiple rounds of freezing and thawing. (lexology.com)
  • Cryopreservation refers to the process of freezing different types of biological material at extreme temperatures as a means of preserving the material in question. (forensisgroup.com)
  • Human egg freezing or oocyte cryopreservation is a novel technique intended to preserve a woman's eggs for later use in life. (send2press.com)
  • Fertility Specialists of Texas provides advanced fertility services to the greater North Texas area, including in vitro fertilization (IVF), donor sperm and donor eggs, embryo freezing or cryopreservation, ICSI, embryo donation and other advanced treatments for both male and female infertility. (send2press.com)
  • For women age 41-55, the ability to preserve their eggs (and thus their future fertility) through egg freezing, oocyte cryopreservation, and egg harvesting gives them more flexibility as to when they can start their families. (indiahospitaltour.com)
  • Given the magnitude of the need, clinicians around the world have raced to develop a Oocyte cryopreservation technique for successful egg-freezing, and, beginning in 2002, promising results ranging from 20-40% successful pregnancy rate (on par with a woman's natural peak fertility rate) were published. (indiahospitaltour.com)
  • These experiments demonstrate that ovary cryopreservation can be a very useful option for banking mouse germplasm, or managing subfertile animal colonies, when embryo or sperm freezing cannot be used or is not cost effective. (jax.org)
  • DALLAS, Texas, May 23, 2011 (SEND2PRESS NEWSWIRE) - Women delaying childbearing or requiring fertility preservation prior to undergoing treatment now have an option with the launch of oocyte cryopreservation at Fertility Specialists of Texas. (send2press.com)
  • Embryo cryopreservation is fairly economical since preservation, unfreezing and embryo transfer costs are much lower compared to the cost of a new donor stimulation and egg collection. (olgafertilityclinic.com)
  • The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for future use. (emoryhealthcare.org)
  • At the Emory Reproductive Center, the mechanical process of embryo cryopreservation is laboratory controlled to reduce the risk of technical failure. (emoryhealthcare.org)
  • The Ethics Committee of the American Society of Reproductive Medicine has suggested the following advantages of cryopreservation. (emoryhealthcare.org)
  • Cryopreservation expert consulting may provide essential information for cases involving such areas as stem cell research, in vitro fertilization (IVF) and other reproductive issues, plants, and other types of organic tissues. (forensisgroup.com)
  • While cryopreservation is used for numerous medical purposes, among other scientific areas of research, it is most commonly utilized for reproductive issues. (forensisgroup.com)
  • With considerable progress made in the success rate of pregnancy in storage for reproductive materials for long-terms, the global cryopreservation for in-vitro fertilization (IVF) market is likely to observe growth over the timeframe of analysis, from 2020 to 2030. (transparencymarketresearch.com)
  • Development of novel cryopreservation methods and increased adoption of assisted reproductive technology are likely to drive therapeutic applications in the IVF sector. (transparencymarketresearch.com)
  • METHODS: First-time IVF patients undergoing elective single embryo transfer and having at least one extra non-transferred embryo suitable for cryopreservation were offered enrollment in the study. (ox.ac.uk)
  • Patients in group B (n=48) had embryos assessed by morphology alone, with only good morphology embryos considered suitable for cryopreservation. (ox.ac.uk)
  • The report also calculates the forthcoming status of Cryopreservation Equipment in Stem Cells market based on thorough analysis. (openpr.com)
  • Cryopreservation is the process of preserving cells or complete tissues that are susceptible to damage caused by chemical reactivity and harmful enzymatic activity. (openpr.com)
  • Top Companies involved in the Global Cryopreservation Equipment in Stem Cells Market are Thermo Fisher Scientific, Charter Medicals, Linde Gas Cryoservices, praxair and other. (openpr.com)
  • Comprehensive assessment of all opportunities and risk in Cryopreservation Equipment in Stem Cells market. (openpr.com)
  • Cryopreservation Equipment in Stem Cells market recent innovations and major events. (openpr.com)
  • Detailed study of business strategies for growth of Cryopreservation Equipment in Stem Cells market-leading players. (openpr.com)
  • Conclusive study about the growth plot of Cryopreservation Equipment in Stem Cells market for forthcoming years. (openpr.com)
  • Finally, all aspects of the Global Cryopreservation Equipment in Stem Cells Market are quantitatively as well qualitatively assessed to study the Global as well as regional market comparatively. (openpr.com)
  • On July 5, 2016, the Federal Circuit vacated and remanded a district court decision holding that U.S. Patent No. 7,604,929 , which is directed to a method of cryopreservation of hepatocytes (liver cells) was invalid under 35 U.S.C. § 101 because it was directed to a patent-ineligible concept. (lexology.com)
  • Cryopreservation is also used for preserving stem cells, which may be utilized in stem cell therapy for the prevention and treatment of different diseases and other medical conditions. (forensisgroup.com)
  • With a CAGR of 23.7%, global market value for cryopreservation equipments used in stem cells industry is anticipated to worth US$2.2 billion by 2015. (marketresearchreports.biz)
  • The growth of the global cryopreservation for in-vitro fertilization (IVF) market has observed a considerable drop in the year 2020 owing to the outbreak of Covid-19. (transparencymarketresearch.com)
  • This factor is likely to support expansion of the global cryopreservation for in-vitro fertilization (IVF) market in the years to come. (transparencymarketresearch.com)
  • Repeated ovarian stimulation in IVF treatments comes with health risks is anticipated to foster development of the global cryopreservation for in-vitro fertilization (IVF) market over the assessment timeline, from 2020 to 2030. (transparencymarketresearch.com)
  • Application, type, and region are important factors in the market that has been considered for the classification of the global cryopreservation for in-vitro fertilization (IVF) market in the near future. (transparencymarketresearch.com)
  • In addition, players in the global cryopreservation for in-vitro fertilization (IVF) market are focusing on the expansion of infrastructure and strategic collaborations to better access to consumers. (transparencymarketresearch.com)
  • The below-mentioned factors are projected to give a glimpse into the nature of the business pertaining to the global cryopreservation for in-vitro fertilization (IVF) market over the assessment period, from 2020 to 2030. (transparencymarketresearch.com)
  • In addition, increased investment into the techniques of development for assisted reproduction of cancer-stricken patients is likely to favor growth of the global cryopreservation for in-vitro fertilization (IVF) market in the near future. (transparencymarketresearch.com)
  • Asia Pacific is likely to come up as one of the leading regions in the global cryopreservation for in-vitro fertilization (IVF) market. (transparencymarketresearch.com)
  • Oocyte cryopreservation will allow those who need to resort to egg donation (the use of someone else's eggs due to the diminished fertility potential of their own eggs) more affordable female fertility treatment options. (indiahospitaltour.com)
  • Cryopreservation is a method of preserving the eggs or embryos for later use. (ivfsurrogacy.in)
  • The main aim of the process is that the woman undergoing the oocyte cryopreservation can use her eggs at any time in the future by thawing them. (ivfsurrogacy.in)
  • Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. (calpoly.edu)
  • Reduction of the risk of triplets or quadruplets by using cryopreservation to store embryos that exceed an optimal number for transfer. (emoryhealthcare.org)
  • Cryopreservation equipment include drystore freezers, mechanical freezers, cryopreservation freezers, cryopreservation vials, incubators and stem cell research laboratory equipment. (openpr.com)
  • Extending the dormant bud cryopreservation method to new tree species. (cgiar.org)
  • This process is performed in an apparatus known as cryopreservation equipment. (openpr.com)
  • These equipment parts help in completing the process of cryopreservation by providing stable cryogenic storage for biological specimens. (openpr.com)
  • Finally, the Court explained that this case differs because the art taught that cryopreservation steps could only be performed once the cellular viability was compromised, and the claimed process was directed to repetition of cryopreservation steps. (lexology.com)
  • Embryo cryopreservation is the process of preserving an embryo at sub-zero temperatures, generally at an embryogenesis stage corresponding to pre-implantation. (olgafertilityclinic.com)
  • If you are searching for the best fertility centre that provides an affordable cost for cryopreservation, then there is no better option than Go IVF Surrogacy. (ivfsurrogacy.in)