Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
Substances that provide protection against the harmful effects of freezing temperatures.
The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
The transformation of a liquid to a glassy solid i.e., without the formation of crystals during the cooling process.
A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations.
Liquids transforming into solids by the removal of heat.
A method of providing future reproductive opportunities before a medical treatment with known risk of loss of fertility. Typically reproductive organs or tissues (e.g., sperm, egg, embryos and ovarian or testicular tissues) are cryopreserved for future use before the medical treatment (e.g., chemotherapy, radiation) begins.
A colorless, odorless, viscous dihydroxy alcohol. It has a sweet taste, but is poisonous if ingested. Ethylene glycol is the most important glycol commercially available and is manufactured on a large scale in the United States. It is used as an antifreeze and coolant, in hydraulic fluids, and in the manufacture of low-freezing dynamites and resins.
A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.
The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.
The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.
Centers for acquiring and storing semen.
Clinical and laboratory techniques used to enhance fertility in humans and animals.
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.
Centers for acquiring, characterizing, and storing organs or tissue for future use.
The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.
The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
Procedures to obtain viable sperm from the male reproductive tract, including the TESTES, the EPIDIDYMIS, or the VAS DEFERENS.
The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.
An OOCYTE-containing structure in the cortex of the OVARY. The oocyte is enclosed by a layer of GRANULOSA CELLS providing a nourishing microenvironment (FOLLICULAR FLUID). The number and size of follicles vary depending on the age and reproductive state of the female. The growing follicles are divided into five stages: primary, secondary, tertiary, Graafian, and atretic. Follicular growth and steroidogenesis depend on the presence of GONADOTROPINS.
The span of viability of a tissue or an organ.
A trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, and sweetening agent.
Derivatives of propylene glycol (1,2-propanediol). They are used as humectants and solvents in pharmaceutical preparations.
The capacity to conceive or to induce conception. It may refer to either the male or female.
Cessation of ovarian function after MENARCHE but before the age of 40, without or with OVARIAN FOLLICLE depletion. It is characterized by the presence of OLIGOMENORRHEA or AMENORRHEA, elevated GONADOTROPINS, and low ESTRADIOL levels. It is a state of female HYPERGONADOTROPIC HYPOGONADISM. Etiologies include genetic defects, autoimmune processes, chemotherapy, radiation, and infections.
An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
The ratio of the number of conceptions (CONCEPTION) including LIVE BIRTH; STILLBIRTH; and fetal losses, to the mean number of females of reproductive age in a population during a set time period.
Methods pertaining to the generation of new individuals, including techniques used in selective BREEDING, cloning (CLONING, ORGANISM), and assisted reproduction (REPRODUCTIVE TECHNIQUES, ASSISTED).
Procedures to obtain viable OOCYTES from the host. Oocytes most often are collected by needle aspiration from OVARIAN FOLLICLES before OVULATION.
Inability to reproduce after a specified period of unprotected intercourse. Reproductive sterility is permanent infertility.
Damages to the EMBRYO, MAMMALIAN or the FETUS before BIRTH. Damages can be caused by any factors including biological, chemical, or physical.
An organic amine proton acceptor. It is used in the synthesis of surface-active agents and pharmaceuticals; as an emulsifying agent for cosmetic creams and lotions, mineral oil and paraffin wax emulsions, as a biological buffer, and used as an alkalizer. (From Merck, 11th ed; Martindale, The Extra Pharmacopoeia, 30th ed, p1424)
The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.
The solid substance formed by the FREEZING of water.
A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.
Endometrial implantation of EMBRYO, MAMMALIAN at the BLASTOCYST stage.
Cytoplasm stored in an egg that contains nutritional reserves for the developing embryo. It is rich in polysaccharides, lipids, and proteins.
A count of SPERM in the ejaculum, expressed as number per milliliter.
Diminished or absent ability of a female to achieve conception.
A solid form of carbon dioxide used as a refrigerant.
The cap-like structure covering the anterior portion of SPERM HEAD. Acrosome, derived from LYSOSOMES, is a membrane-bound organelle that contains the required hydrolytic and proteolytic enzymes necessary for sperm penetration of the egg in FERTILIZATION.
Undifferentiated cells resulting from cleavage of a fertilized egg (ZYGOTE). Inside the intact ZONA PELLUCIDA, each cleavage yields two blastomeres of about half size of the parent cell. Up to the 8-cell stage, all of the blastomeres are totipotent. The 16-cell MORULA contains outer cells and inner cells.
The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.
The emission of SEMEN to the exterior, resulting from the contraction of muscles surrounding the male internal urogenital ducts.
A nonreducing disaccharide composed of GLUCOSE and FRUCTOSE linked via their anomeric carbons. It is obtained commercially from SUGARCANE, sugar beet (BETA VULGARIS), and other plants and used extensively as a food and a sweetener.
Below normal weather temperatures that may lead to serious health problems. Extreme cold is a dangerous situation that can bring on health emergencies in susceptible people.
Results of conception and ensuing pregnancy, including LIVE BIRTH; STILLBIRTH; SPONTANEOUS ABORTION; INDUCED ABORTION. The outcome may follow natural or artificial insemination or any of the various ASSISTED REPRODUCTIVE TECHNIQUES, such as EMBRYO TRANSFER or FERTILIZATION IN VITRO.
The event that a FETUS is born alive with heartbeats or RESPIRATION regardless of GESTATIONAL AGE. Such liveborn is called a newborn infant (INFANT, NEWBORN).
A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.
Methods for maintaining or growing CELLS in vitro.
A plant genus of the family Gentianaceae whose members contain SECOIRIDOIDS and have been used in TRADITIONAL MEDICINE for suppressing INFLAMMATION.
The heat flow across a surface per unit area per unit time, divided by the negative of the rate of change of temperature with distance in a direction perpendicular to the surface. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.
Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION.
The inability of the male to effect FERTILIZATION of an OVUM after a specified period of unprotected intercourse. Male sterility is permanent infertility.
The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.
Solutions used to store organs and minimize tissue damage, particularly while awaiting implantation.
The process of protecting various samples of biological material.
Morphological and physiological development of EMBRYOS.
A condition of suboptimal concentration of SPERMATOZOA in the ejaculated SEMEN to ensure successful FERTILIZATION of an OVUM. In humans, oligospermia is defined as a sperm count below 20 million per milliliter semen.
Elements of limited time intervals, contributing to particular results or situations.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
Techniques for the artifical induction of ovulation, the rupture of the follicle and release of the ovum.
Transfer of preovulatory oocytes from donor to a suitable host. Oocytes are collected, fertilized in vitro, and transferred to a host that can be human or animal.
The fusion of a spermatozoon (SPERMATOZOA) with an OVUM thus resulting in the formation of a ZYGOTE.
Any of various ruminant mammals of the order Bovidae. They include numerous species in Africa and the American pronghorn.
An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.
The fertilized OVUM resulting from the fusion of a male and a female gamete.
A plant genus of the family ARECACEAE. It is a tropical palm tree that yields a large, edible hard-shelled fruit from which oil and fiber are also obtained.
The pressure required to prevent the passage of solvent through a semipermeable membrane that separates a pure solvent from a solution of the solvent and solute or that separates different concentrations of a solution. It is proportional to the osmolality of the solution.
A family of marsupials in the order Diprotodontia, native to Australia and possessing vestigial tails. There is a single living genus and species: Phascolarctos cinereus, the koala.
Morphological and physiological development of EMBRYOS or FETUSES.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
The procedure of removing TISSUES, organs, or specimens from DONORS for reuse, such as TRANSPLANTATION.
Aquaporin 3 is an aquaglyceroporin that is expressed in the KIDNEY COLLECTING DUCTS and is constitutively localized at the basolateral MEMBRANE.
The capability of bearing live young (rather than eggs) in nonmammalian species. Some species of REPTILES and FISHES exhibit this.
An absence of warmth or heat or a temperature notably below an accustomed norm.
Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The techniques used to select and/or place only one embryo from FERTILIZATION IN VITRO into the uterine cavity to establish a singleton pregnancy.
A branch of embryology for the study of congenital malformations and developmental abnormalities.
Facilities that collect, store, and distribute tissues, e.g., cell lines, microorganisms, blood, sperm, milk, breast tissue, for use by others. Other uses may include transplantation and comparison of diseased tissues in the identification of cancer.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The condition of carrying two or more FETUSES simultaneously.
A complication of OVULATION INDUCTION in infertility treatment. It is graded by the severity of symptoms which include OVARY enlargement, multiple OVARIAN FOLLICLES; OVARIAN CYSTS; ASCITES; and generalized EDEMA. The full-blown syndrome may lead to RENAL FAILURE, respiratory distress, and even DEATH. Increased capillary permeability is caused by the vasoactive substances, such as VASCULAR ENDOTHELIAL GROWTH FACTORS, secreted by the overly-stimulated OVARIES.

Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay. (1/2910)

The sperm chromatin structure assay (SCSA) is a flow cytometric (FCM) technique which exploits the metachromatic properties of Acridine Orange to monitor the susceptibility of sperm chromatin DNA to in-situ acid denaturation. SCSA was used to study the chromatin structure variations of human spermatozoa in semen, both before and after swim-up and after cryopreservation. Semen samples were provided by 19 healthy normozoospermic subjects attending pre-marriage checks. Each sample was divided into three aliquots: the first aliquot was evaluated without further treatment, the second underwent swim-up, and the third was stored according to standard cryopreservation techniques in liquid nitrogen at -196 degrees C. Samples were also analysed by light and fluorescence microscopy (after Acridine Orange staining to evaluate the number of green fluorescent sperm heads), and by computer-assisted semen analysis. The results showed that post-rise spermatozoa represent a subpopulation characterized by a general improvement of the morphological (reduction of the percentage of abnormal forms and heads, increase of the green head sperm percentage) and kinetic parameters. This subpopulation also exhibited improved chromatin structure properties, confirming that these cells have the best structural and functional characteristics, indicative of optimal fertilizing ability. On the other hand, overall sperm quality deteriorates after cryopreservation. When thawed spermatozoa underwent an additional swim-up round, a general improvement of nuclear maturity was seen in the post-rise spermatozoa.  (+info)

Arterial damage induced by cryopreservation is irreversible following organ culture. (2/2910)

OBJECTIVES: The aim of the present study was to investigate the changes which occur to the arterial wall following cryopreservation and thawing and to determine whether these changes are reversible after a week of culture in an organ bath. MATERIALS AND METHODS: Rat iliac arterial segments were cryopreserved. Once thawed, the arterial segments were cultured for a period of 0, 1, 2, 4 or 7 days. Freshly isolated rat iliac vessels cultured for 7 days served as the control group. Evaluation was made of ultrastructural changes, the expression of metalloproteinase activity (MMP-1, MMP-3 and MMP-9) and the apoptotic state of cells. RESULTS: The freezing-thawing process induced damage to the arterial segments compared to fresh control vessels. After 1 week of culture, arteries showed a high degree of tissue degeneration. Only a few individual endothelial cells remained on the luminal surface. There was a gradual increase in the proportion of apoptotic cells. The sequential expression of MMP-1 during the first 2 days and subsequent expression of MMP-3 and MMP-9 were of most significance. CONCLUSIONS: Cryopreservation induced damage to the vessels which could not be reversed by organ culture. The changes observed in the expression of metalloproteinases may be indicative of the degenerative process which occurs in the extracellular matrix.  (+info)

Effect of cryopreservation on cytochrome P-450 enzyme induction in cultured rat hepatocytes. (3/2910)

In the present study, we evaluated the inducibility of cytochrome P-450 (CYP) CYP1A, CYP2B, CYP3A, and CYP4A by beta-naphthoflavone, phenobarbital, dexamethasone, and clofibric acid, respectively, in primary hepatocyte cultures prepared from both fresh and cryopreserved rat hepatocytes. Rat hepatocytes were successfully thawed and cultured after cryopreservation in liquid nitrogen for up to 1 month. Percentage of total recovery, viable cell recovery, and final viability of the cells were 68%, 72%, and 85%, respectively. Regardless of whether they were cryopreserved or not, cultured hepatocytes exhibited near-normal morphology. Treatment of cryopreserved hepatocytes with beta-naphthoflavone caused an 8-fold increase in 7-ethoxyresorufin O-dealkylase (CYP1A1/2) activity, with an EC50 of 1.5 microM; treatment with phenobarbital caused a 26-fold increase in 7-pentoxyresorufin O-dealkylase (CYP2B1/2) activity, with an EC50 of 10 microM; treatment with dexamethasone caused a 10-fold increase in testosterone 6beta-hydroxylase (CYP3A1/2) activity, with an EC50 of 1.3 microM, whereas treatment with clofibric acid caused a 3-fold increase in lauric acid 12-hydroxylase (CYP4A1-3) activity, with an EC50 of 170 microM. The induction of CYP1A, CYP2B, CYP3A, and CYP4A enzymes by these inducers was confirmed by Western immunoblotting. The patterns of P-450 induction in cryopreserved rat hepatocytes, in terms of concentration response, reproducibility, magnitude, and specificity of response, were similar to those observed in freshly isolated hepatocytes. Additionally, the magnitude and specificity of induction was similar to that observed in vivo in rats. In conclusion, under the conditions examined, cryopreserved rat hepatocytes appear to be a suitable in vitro system for evaluating xenobiotics as inducers of P-450 enzymes.  (+info)

Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. (4/2910)

When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane after different cryostorage protocols: i.e. 10% (v/v) glycerol; sperm maintenance medium (MM); freezing medium TEST yolk buffer (TYB); or cryostorage without protection (cryoshock). Using a combination of two fluorescent dyes, annexin V and propidium iodide (PI), led to three groups of spermatozoa being identified: (i) viable spermatozoa (annexin V-negative and PI-negative); (ii) dead spermatozoa (annexin V-positive and PI-positive); and (iii) cells with impaired but integer plasma membrane (annexin V-positive and PI-negative). The percentage of vital annexin V-negative spermatozoa increased significantly (P < 0.05) from spermatozoa treated by cryoshock (15.0+/-1.2%) to spermatozoa cryopreserved by TYB (26.6+/-2.2%) via cryopreservation by 10% (v/v) glycerol (19.9+/-1.6%) and by MM (22.2 1.8%) and was associated with the percentage of motile spermatozoa (17.6+/-3.4% by glycerol; 19.6+/-3.7% by MM and 22.6+/-3.9% by TYB; P = 0.0001). Of the spermatozoa, 12-22% were annexin V-positive even though they did not bind to PI, indicating viability before as well as after cryostorage. The percentage of vital annexin V-positive spermatozoa was significantly correlated with different sperm motility parameters (velocity straight linear, r = 0.601, P = 0.018; percentage of linearly motile spermatozoa: r = 0.549, P = 0.034). We, therefore, concluded that annexin V-binding is more sensitive in detecting a deterioration of membrane functions than PI staining, and that a considerable percentage of spermatozoa might have dysfunctional plasma membranes besides dead or moribund cells. Of the cryopreservation protocols tested, TYB yielded the most viable spermatozoa. Therefore, we advocate the use of the annexin V-binding assay for the evaluation of the quality and integrity of spermatozoa.  (+info)

Freezer anthropology: new uses for old blood. (5/2910)

Archived blood fractions (plasma, settled red cells, white cells) have proved to be a rich and valuable source of DNA for human genetic studies. Large numbers of such samples were collected between 1960 and the present for protein and blood group studies, many of which are languishing in freezers or have already been discarded. More are discarded each year because the usefulness of these samples is not widely understood. Data from DNA derived from 10-35-year-old blood samples have been used to address the peopling of the New World and of the Pacific. Mitochondrial DNA haplotypes from studies using this source DNA support a single wave of migration into the New World (or a single source population for the New World), and that Mongolia was the likely source of the founding population. Data from Melanesia have shown that Polynesians are recent immigrants into the Pacific and did not arise from Melanesia.  (+info)

Preparation of endometrium for egg donation. (6/2910)

Nowadays oocyte donation is a well established method of assisted reproduction and offers the unique opportunity to treat patients with various clinical indications, with or without ovarian function, in a novel way. In women with ovarian failure, artificial menstrual cycles are required before proceeding to oocyte donation. Oestrogen may be delivered in the form of oral tablets, transdermal patches in order to bypass the gastrointestinal tract thus avoiding first pass metabolism and by vaginal application. Our regimen is oestradiol valerate given in various concentrations, in order to mimic the regular cyclic fluctuations throughout the cycle. Progesterone may be administered in the form of oral tablets, intravaginal suppositories or rings and i.m. injections. Our results, as of most other groups, strongly support the vaginal route of progesterone administration. In women with retained ovarian function, synchronization of donor-recipient cycle presents a special problem, as there is strong evidence that a temporal window of maximal endometrial receptivity exists. Cryopreservation of donated embryos may be used to overcome the problem, but this approach has the important drawback of embryonic loss occurring after freezing and thawing. The method of choice is the administration of gonadotrophin-releasing hormone agonists (GnRHa) to render the patients functionally agonadal in order to circumvent cycle asynchrony between the donor and recipient.  (+info)

Turner's syndrome and pregnancies after oocyte donation. (7/2910)

A total of 20 clinical pregnancies was achieved among 18 women with Turner's syndrome who were treated in an oocyte donation programme. The oocytes were donated by voluntary unpaid donors. A mean of 1.8 embryos per transfer was given to each recipient by way of 28 fresh and 25 frozen embryo transfers. With fresh and frozen embryos, 13 and seven pregnancies respectively were achieved. The clinical pregnancy rate per fresh embryo transfer was 46%, and the implantation rate 30%, being similar to the corresponding rates among our oocyte recipients with primary ovarian failure in general. The corresponding rates with frozen embryos were 28 and 19%. Of these pregnancies, 40% ended in miscarriage. This high rate may be explained by uterine factors. Six women were hypertensive during pregnancy, a rate comparable with that in other oocyte donation pregnancies. All these women delivered by Caesarean section. Pregnancy and implantation rates after oocyte donation were high in women with Turner's syndrome, but the risk of cardiovascular and other complications is high. Careful assessment before and during follow-up of pregnancy are important. Transfer of only one embryo at a time to avoid the additional complications caused by twin pregnancy is recommended.  (+info)

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing. (8/2910)

Uncontrolled-rate freezing techniques represent an attractive alternative to controlled-rate cryopreservation procedures which are time-consuming and require high-level technical expertise. In this study, we report our experience using uncontrolled-rate cryopreservation and mechanical freezer storage at -140 degrees C. Twenty-eight PBPC samples (10 cryovials, 18 freezing bags) from 23 patients were cryopreserved in a cryoprotectant solution composed of phosphate-buffered saline (80%, v/v) supplemented with human serum albumin (10%, v/v) and dimethylsulfoxide (10%, v/v). The cryopreservation procedure required on average 1.5 h. The mean (+/- s.e.m.) storage time of cryovials and bags was 344+/-40 and 299+57 days, respectively. Although cell thawing was associated with a statistically significant reduction of the absolute number of nucleated cells (vials: 0.3x10(9) vs. 0.2x10(9), P< or =0.02; bags: 14x10(9) vs. 11x10(9), P< or =0.0003), the growth of committed progenitors was substantially unaffected by the freezing-thawing procedure, with mean recoveries of CFU-Mix, BFU-E, and CFU-GM ranging from 60+/- 29% to 134+/-15%. Mean recoveries of LTC-IC from cryovials and bags were 262+/-101% and 155+/-27% (P< or =0.2), respectively. In 14 out of 23 patients who underwent high-dose chemotherapy and PBPC reinfusion, the pre-and post-freezing absolute numbers of hematopoietic progenitors cryopreserved in bags were compared. A significant reduction was detected for CFU-Mix (11 vs. 7.4x10(5)), but no significant loss of BFU-E (180 vs. 150x10(5)), CFU-GM (400 vs. 290x10(5)) and LTC-IC (15 vs. 16x10(5)) could be demonstrated. When these patients were reinfused with uncontrolled-rate cryopreserved PBPC, the mean number of days to reach 1x10(9)/l white blood cells and 50x10(9)/l platelets were 9 and 13, respectively. In conclusion, the procedure described here is characterized by short execution time, allows a substantial recovery of primitive and committed progenitors and is associated with prompt hematopoietic recovery following myeloablative therapy even after long-term storage.  (+info)

PromoCell. *Note: Additional companies can be included on request. Moreover, customized report can be available as per the clients wishes or specific needs.. Get free exclusive sample of Cryopreservation Media market report @ https://dataintelo.com/request-sample/?reportId=13252. The report involves an extensive study of the data available for the global Cryopreservation Media market during the historical period, 2015-2019 and makes a robust assessment of the market performance and trend for the base year, 2020. It is an in-depth analysis report of the market that offers vital insights on industry growth opportunities and development, drivers, challenges, and restrains for the global Cryopreservation Media market during the forecast period, 2021-2028.. According to analysis, the global Cryopreservation Media market was valued at USD XX million in 2019 and is projected to reach approximately USD XX million by the end of 2028, expanding at a CAGR of XX% during the forecast period ...
Embryo cryopreservation involves the generation of pre-implantation embryos using males provided by the investigator and the controlled rate cooling of collected embryos to -30°C followed by rapid freezing of embryos in liquid nitrogen to -196°C.. While embryo cryopreservation is the gold standard for preserving mouse germplasm, not all mouse strains are suitable for embryo cryopreservation. However, we have been successful at cryopreserving embryos from several different mouse strains.. Embryos for cryopreservation can be obtained in two basic ways: ...
Anderson J, Toh ZQ, Reitsma A, Do LAH, Nathanielsz J, Licciardi PV. Effect of peripheral blood mononuclear cell cryopreservation on innate and adaptive immune responses. Journal of immunological methods (2018) PubMed ...
There are many benefits of oocyte and embryo cryopreservation. When used as part of the IVF treatment process, cryopreservation can increase the likelihood of achieving pregnancy for many patients. Cryopreservation also allows couples to continue to see their families grow as IVF cycles can be repeated, years later, from the same group of eggs originally recovered for treatment.. During an IVF cycle, between 10 and 30 eggs can typically be recovered from the ovary. While it is likely that not all of these eggs will be deemed appropriate for cryopreservation, the process frequently allows couples to save a number of eggs for multiple conception attempts. In some cases, this can lead to successful treatment without the use of fertility drugs. It also reduces the likelihood that another egg retrieval procedure will be required. The number of embryos transferred depends on the number of eggs available, age, and other factors that are unique to each patient. In some cases, cryopreservation makes ...
Oocyte Cryopreservation abroad in India offers info on cost Oocyte Cryopreservation India,Oocyte Cryopreservation IVF Doctors &hospitals India.
OBJECTIVE: During IVF, non-transferred embryos are usually selected for cryopreservation on the basis of morphological criteria. This investigation evaluated an application for array comparative genomic hybridization (aCGH) in assessment of surplus embryos prior to cryopreservation. METHODS: First-time IVF patients undergoing elective single embryo transfer and having at least one extra non-transferred embryo suitable for cryopreservation were offered enrollment in the study. Patients were randomized into two groups: Patients in group A (n=55) had embryos assessed first by morphology and then by aCGH, performed on cells obtained from trophectoderm biopsy on post-fertilization day 5. Only euploid embryos were designated for cryopreservation. Patients in group B (n=48) had embryos assessed by morphology alone, with only good morphology embryos considered suitable for cryopreservation. RESULTS: Among biopsied embryos in group A (n=425), euploidy was confirmed in 226 (53.1%). After fresh single embryo
The objectives of this course are that at the conclusion of this course students should have a good understanding of: Folliculogenesis and Fertilization; Preimplantation Embryology; sperm and oocytes retrieval; criteria of selected or scoring the gametes and zygotes for cryopreservation; liquid nitrogen handling; principles of cryobiology. Students should be aware of the general aspects and implication of the cryobiology research and the potentiality that this represents for clinical application; The principles of cryobiology; The Cryoprotectants additives and how they protect the cells by stabilizing intracellular proteins; The factors that affect cellular response to freezing; The different cryopreservation protocols and what is ongoing in this field; The cross-contamination of samples in liquid nitrogen; problems in achieving a good result of cryopreservation procedure; The possible epigenetic effects of the cryopreservation procedure; testicular and ovarian tissue cryopreservation ...
Our test system specialists make each preparation with strict quality controls. Learn about our fresh and cryopreserved cell products for in vitro assays.
TY - JOUR. T1 - The use of physical and virtual infrastructures for the validation of algal cryopreservation methods in international culture collections. AU - Day, John. AU - Lorenz, Maike. AU - Wilding, Tom. AU - Friedl, Thomas. AU - Harding, Keith. AU - Pröschold, Thomas. AU - Brennan, Debra. AU - Müller, Julia. AU - Santos, Lilia. AU - Santos, Fatima. AU - Osorio, Uugo. AU - Amaral, Raquel. AU - Elster, Josef. AU - Lukavský, Jaromir. AU - Lukesova, Alena. AU - Hrouzek, P. AU - Lukes, M. AU - Probert, Ian. AU - Ryan, M.J.. AU - Benson, Erica E. PY - 2007. Y1 - 2007. N2 - Two cryopreservation methods, colligative cryoprotection coupled with controlled cooling and vitrification-based, encapsulation-dehydration were validated by five members of the EU Research Infrastructure consortium, COBRA, and two independent external validators. The test strain Chlorella vulgaris SAG 211-11b was successfully cryopreserved using two-step cooling employing passive (Mr Frosty((R))) and Controlled Rate ...
Populations of Redside Dace Clinostomus elongatus have declined in many areas across the speciesNorth American range. Therefore, the development of sperm cryopreservation technology would provide an invaluable means of preserving genetic diversity in populations that are in imminent danger of extirpation.We developed cryopreservation protocols by testing the effects of diluent (buffered sperm motility-inhibiting saline solution [BSMIS]; BSMIS + glycine; sucrose; and Hanks balanced salt solution [HBSS]), cryoprotectant (dimethyl sulfoxide [DMSO]; propylene glycol [PG]; N,N-dimethylacetamide [DMA]; and methanol), freezing rate (1, 5, and 10◦C/min), and male-to-male variation on sperm quality. Incubating sperm in extenders affected motility;BSMIS + glycine + methanol,BSMIS + glycine + PG, and HBSS + methanol were the only treatments for which motility was not significantly different from that of fresh sperm. Sperm frozen with sucrose had higher motility than sperm frozen with BSMIS + glycine, ...
Development of effective cryopreservation protocols will be essential to realizing the potential for clinical application of neural stem and progenitor cells. Current cryopreservation protocols have been largely employed in research, which does not require as stringent consideration of viability and sterility. Therefore, these protocols involve the use of serum and protein additives, which can potentially introduce contaminants, and slow cooling with DMSO/glycerol-based cryopreservation solutions, which impairs cell survival. We investigated whether serum- and protein-free vitrification is effective for functional cryopreservation of neurosphere cultures of neural stem or progenitor cells. To protect the samples from introduction of other contaminants during handling and cryostorage, an original straw-in-straw method (250 l sterile straw placed in 500 l straw) for direct immersion into liquid nitrogen and storing the samples was also introduced. The protocol employed brief step-wise ...
Find the best embryo cryopreservation doctors in Mumbai. Get guidance from medical experts to select embryo cryopreservation specialist in Mumbai from trusted hospitals - credihealth.com
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
TY - JOUR. T1 - Cryopreservation of human prophase I oocytes collected from unstimulated follicles. AU - Toth, T. L.. AU - Lanzendorf, S. E.. AU - Sandow, B. A.. AU - Veeck, L. L.. AU - Hassen, W. A.. AU - Hansen, K.. AU - Hodgen, G. D.. PY - 1994. Y1 - 1994. N2 - Objective: To evaluate the cryopreservation of immature human oocytes obtained from unstimulated ovarian tissue. Design: Immature prophase I oocytes were obtained from unstimulated follicles and were either cryopreserved or cultured as controls. Cryopreservation was performed in a programmable freezing machine using one of two protocols. Method I (n = 133) used a one-step addition of cryoprotectant followed by a slow freeze and thaw protocol. With method II (n = 95), the cryoprotectant was added in a stepwise manner with cryopreservation performed in the presence of 0.2 M sucrose followed by rapid freezing and thawing. Setting: Basic research center at a medical school. Patients: Patients undergoing oophorectomy for nonovarian ...
Background Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols in different species that would allow for an efficient storage of semen samples for long periods of time, ensuring its viability. Nowadays, it is widely known that an important individual component exists in the cryoresistance of semen, and efforts are aimed at identifying those sperm characteristics that may allow us to predict this cryoresistance. This knowledge would lead, ultimately, to the design of optimized freezing protocols for the sperm characteristics of each male. Methodology/Principal Findings We have evaluated the changes that occur in the sperm head dimensions throughout the cryopreservation process. We have found three different patterns of response, each of one related to a different sperm quality at thawing. We have been able to characterize males based on these patterns. For each male, its pattern remained constant among
Vitrification is an ultra‐rapid cryopreservation technique that provides excellent survivability by using optimal concentrations of cryoprotectants used in a step‐wise process supporting rapid dehydration of the oocyte. The dehydrated oocyte is loaded into a thin storage device that will facilitate ultra‐rapid cooling of the oocyte when plunged into liquid nitrogen - eliminating concerns of damaging ice‐crystal formation associated with traditional slow‐freezing procedures. Thaw solutions are formulated to support rehydration through the rapid warming process that follows vitrification to optimize cellular survival.. Irvine Scientifics vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. These solutions support implantation and pregnancy rates comparable to those of fresh cycles.. ...
Ovarian tissue cryopreservation and transplantation is a real option to preserve and restore fertility in young cancer patients. However, there is a concern regarding the possible presence of malignant cells in the ovarian tissue, which could lead to recurrence of the primary disease after reimplantation. A review of the existing literature was done to evaluate the risk of transplanting malignant cells in case of the main malignant indications for ovarian tissue cryopreservation. For ovarian tissue from patients with hematologic malignancies, it is of paramount importance to identify minimal residual disease before ovarian tissue transplantation. Indeed, these pathologies, reviewed here in detail, are considered to be most at risk of ovarian metastasis ...
The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for future use.
Global Stem Cells Cryopreservation Equipments Market: This market research report focuses on Past-Current Size, Price, Trends, Shares, Segment & Forecast
The aim of this study was to characterize the testicular isoform of angio-tensin-converting enzyme (tACE) before and after semen cryopreservation, and in the acrosome reaction of sperm from Nelore bulls in vitro. Ejaculates of 10 sexually mature Nelore bulls were used. After semen was collected, 1.0 mL of the ejaculate was used for the analysis and the rest was subjected to cryopreservation. Fresh semen before freezing, and frozen/thawed semen were centrifuged twice and the pellet was resuspended intyrodes albumin lactate pyruvate (TALP). Thereafter, 100 μL aliquots containing 100 × 106 spermatozoa were prepared. Aliquots of samples were used for western blot analysis, subjected to capacitation, and thereafter, acrosome reaction assays were performed </>in vitro</i>. With the help of an anti-ACE monoclonal antibody, a 100 kDa protein band was identified in the spermatozoa of Nelore bulls. Cryopreservation reduced the intensity of the protein bands obtained by western blot assay to less
Jenderek, M.M.; Ambruzs, B.; Tanner, J.; Holman, G.; Ledbetter, C.; Postman, J.; Ellis, D.; Leslie, C. 2014. Extending the dormant bud cryopreservation method to new tree species. In: Reed, B.M. (ed). Proceedings of the International Conference. 2. International Symposium on Plant Cryopreservation. Fort Collins (USA). 11-14 Aug 2013. Conference Paper International Society for Horticultural Science (ISHS). ISBN 978-94-62610-27-9. pp. 133-136. Acta Horticulturae. ISSN 0567-7572. no.1039 ...
CryoSearch was created by BioCision in order to help standardize the field of cryopreservation through the sharing of protocols and encouraging discussion about protocols and methods. At BioCision, our mission is to standardize sample handling. While our products help do that, products are only one part of reducing variability. The other part is how those products get used; in other words, protocols. Since we have somewhat of a specialty in cryopreservation, and there arent any other resources like CryoSearch, we thought this would be a great place to start.. Like the site? Wed love for you to check out our cryopreservation products as well. We have leak-proof and barcoded cryovials, a line of containers for slow, controlled-rate cell freezing, and tube racks that ensure precise temperature control and can also be used for snap-freezing as they are liquid-nitrogen compatible. We have a bunch of other products as well, all designed to help make your science more reliable.. Even if youre not in ...
This advisory is written for those who would like to understand the processes of mouse strain cryopreservation, why it is important and how to take advantage of the services offered. The most efficient method to protect mouse strains is by cryopreservation of gametes or embryos. Cryopreservation, and especially re-animation from frozen material, is not a trivial exercise, nor is the establishment of a cryopreservation and IVF laboratory cheap. There are numerous laboratories around the world that offer these services.
If during your egg donation procedure with fresh or frozen donor eggs more embryos were obtained than are going to be transferred into the uterus, these embryos, if of good quality, can be frozen. These frozen embryos are property of the couple or a single woman and can be used for the next attempt or a next pregnancy. They may be required if you have not got pregnant in the fresh try or if you have got pregnant and want a brother or sister for your first child several years later.. The benefit of cryopreservation is that a woman has an additional chance to conceive without new stimulation procedure and follicular puncture in the donor. Also new sperm collection is not required. Hence the transfer of frozen embryos is much less time consuming and very cost effective. It requires only one day visit for a woman and does not require a visit of a man. Preparation of uterus to the embryo transfer does not differ much from the preparation to the fresh embryo transfer.. Embryo cryopreservation is the ...
Embryo cryopreservation or embryo freezing is a method used to preserve embryos by cooling and storing them at low temperatures (-196°C). They can then be thawed at a future date and transferred to the uterus, providing additional opportunity for achieving conception.. As part of the usual process of in vitro fertilization, multiple eggs may be stimulated to grow, be recovered from the ovary and become fertilized. This may result in additional embryos in excess of the number that a couple would desire to have transferred back to the uterus at one time. If the additional embryos are of sufficiently good quality to undergo the process of cryopreservation, this can be performed in order to provide another opportunity for embryo transfer. Depending on the embryo stage at the time of freezing, between 60-90% survive freeze/thaw process resulting in a future pregnancy.. If the IVF fresh embryo transfer does not result in pregnancy, the frozen embryos can be subsequently thawed and transferred to the ...
The aim of this study was to characterize the angiotensin-converting enzyme (ACE) in Gir semen before and after cryopreservation. The ejaculate of five sexually mature bulls was used. After collection, one 1-mL aliquot of fresh semen was analyzed immediately, and the rest of the semen was cryopreserved in liquid nitrogen for subsequent analysis. Freshly collected semen and thawed cryopreserved semen were centrifuged twice with Tyrodes albumin lactate pyruvate medium (TALP) to remove plasma and extender, respectively. Samples were then subjected to western blotting, immunocytochemistry, and enzymatic activity techniques. At least one 100 kDa band was observed in every bull analyzed using western blotting with an anti-ACE monoclonal antibody, and band intensity decreased by 70% (p < 0.05) after cryopreservation. Immunocytochemistry showed periacrosomal ACE localization, and the area stained by the fluorescent antibody significantly decreased (p < 0.05) after cryopreservation. Enzyme activity was
Cryopreserved donor lymphocyte infusion (DLI) products are manufactured and administered to treat relapse after allogeneic hematopoietic stem cell transplantation. Reported clinical responses to DLIs vary broadly, even within the same group of patients. While there is an implicit recognition of the fact that different manufacturing protocols may have specific effects on different cell types, cryopreservation protocols are frequently derived from our experience in the cryopreservation of stem cell products and do not account for the heterogeneous functional nature of DLI T-cell populations. Here, we report the results of a prospective, multicenter trial on the effect of four different cryopreservation solutions that were used to freeze DLIs compared to control DLIs that were refrigerated overnight ...
Abstract: There are many reasons why cryopreservation of gametes are important: 1) maintenance of genetic diversity in domestic and wild species populations (Wildt 1992; Wildt 1997; Critser and Russell 2000), 2) facilitating the distribution of genetically superior domestic species lines, 3) treatment of human infertility (Kuczynski et al. 2001; Ranganathan et al. 2002; Tash et al. 2003; Agarwal et al. 2004; Nalesnik et al. 2004), and 4) genetic banking of genetically modified animal models of human health and disease (Critser and Russell 2000; Knight and Abbott 2002). Although cryopreservation of gametes have been routine in many other livestock industries such as the dairy industry. Cryopreservation in the swine industry is still in it infancy. Birth of live offspring has been reported from cryopreserved sperm and embryos, but success is still extremely low. From an industry perspective the low success rate has too much of an economic impact that the integration of the technology has been ...
Clinica Mar&Gen offers Testicular Biopsy Cryopreservation procedures starting from $13,400 and it is specialized in Reproductive Medicine treatments.
Taconic offers both embryo and sperm cryopreservation. Taconic also offers revitalization of cryopreserved lines. Learn more today.
An undesired side effect of cancer treatment is potential subfertility or infertility. Timely cryopreservation of semen is the best modality to ensure fertility. This retrospective data analysis established the usage rate of cryopreserved semen from cancer patients. Pubertal and post-pubertal patients who could become infertile as a result of cancer ... read more (treatment) were offered the option to cryopreserve semen prior to treatment. Of the 898 patients who cryopreserved their semen in our hospital, 96 (10.7%) used this for assisted reproductive technology. The live birth rates for intrauterine insemination, in-vitro fertilization, intracytoplasmic sperm injection and cryopreserved embryo transfer were 13%, 29%, 32% and 17%, respectively. Of all couples involved, 77% achieved parenthood, i.e. 60 of the 78 patients (with complete follow-up) fathered at least one child. show less ...
With Europe Biobank Week 2018 in Antwerp, Belgium coming up (September 4-7th) people will be reviewing the available information on the subject. A definitive book is published by Springer - and of the fourteen chapters, chapter five, The Future of Cell Preservation Strategies by John M. Baust et al will probably interest our customers.. Dr Baust introduces this subject: ... cryopreservation is often viewed as an old school discipline yet modern cryopreservation is undergoing another scientific and technology development growth phase. In this regard, todays cryopreservation processes and cryopreserved products are found at the forefront of research in the areas of discovery science, stem cell research, diagnostic development and personalised medicine. As the utilisation of cryopreserved cells continues to increase, the demands placed on the biobanking industry are increasing and evolving at an accelerated rate. No longer are samples providing for high immediate post-thaw viability ...
Excessive reactive oxygen species generation during sex sorting and cryopreservation of stallion sperm leads to DNA fragmentation, lipid peroxidation, and motility loss. In this study we investigated whether antioxidant supplementation during sex sorting and cryopreservation could ameliorate the effects of reactive oxygen species on stallion sperm. In experiment 1, the postthaw characteristics of stallion sperm (N = 9) cryopreserved in the presence or absence of catalase (200 U/mL), cysteine (0.2 mg/mL), or quercetin (0.15 mM) was examined. Motility and acrosome integrity were assessed at 0, 1, and 3 hours after thawing. The sperm chromatin structure assay (SCSA; detectable DNA fragmentation index [DFI], mean DFI, and DFI) was used to assess DNA integrity immediately after thawing. Quercetin increased the total postthaw motility (25.3% vs. 20.9%; P | 0.05), but there was no beneficial effect of catalase or cysteine. Based on these results, the effect of quercetin during cryopreservation on the postthaw
DALLAS, Texas (SEND2PRESS NEWSWIRE) -- Women delaying childbearing or requiring fertility preservation prior to undergoing treatment now have an option with the launch of oocyte cryopreservation at Fertility Specialists of Texas. Now there is an option - egg freezing - that enables women to have eggs (also known as oocytes) aspirated before undergoing cancer treatment, and frozen for later use. - News from Fertility Specialists of Texas, issued by Send2Press Newswire
Background Simple and effective cryopreservation of human oocytes would have an enormous impact on the financial and ethical constraints of human assisted reproduction. Recently, studies have demonstrated the potential for cryopreservation in an ice-free glassy state by equilibrating oocytes with high concentrations of cryoprotectants (CPAs) and rapidly cooling to liquid nitrogen temperatures. A major difficulty with this approach is that the high concentrations required for the avoidance of crystal formation (vitrification) also increase the risk of osmotic and toxic damage. We recently described a mathematical optimization approach for designing CPA equilibration procedures that avoid osmotic damage and minimize toxicity, and we presented optimized procedures for human oocytes involving continuous changes in solution composition. Methods Here we adapt and refine our previous algorithm to predict piecewise-constant changes in extracellular solution concentrations in order to make the predicted ...
course on the cryopreservation & transplantation of human ovarian tissue as well as preantral follicle isolation & in vitro culture
Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request ...
Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use.
Germinal vesicle and metaphase I oocytes should be matured in vitro before cryopreservation to optimize the reproductive potential of all retrieved oocytes.
The use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential ...
In the past two decades, laboratories around the world have produced thousands of mutant, transgenic, and wild-type zebrafish lines for biomedical research. Although slow-freezing cryopreservation of zebrafish sperm has been available for 30 years, current protocols lack standardization and yield inconsistent post-thaw fertilization rates. Cell cryopreservation cannot be improved without basic physiological knowledge, which was lacking for zebrafish sperm. The first goal was to define basic cryobiological values for wild-type zebrafish sperm and to evaluate how modern physiological methods could aid in developing improved cryopreservation protocols. Coulter counting methods measured an osmotically inactive water fraction (Vb) of 0.37+/-0.02 (SEM), an isosmotic cell volume (V(o)) of 12.1+/-0.2mum(3) (SEM), a water permeability (L(p)) in 10% dimethyl sulfoxide of 0.021+/-0.001(SEM)mum/min/atm, and a cryoprotectant permeability (P(s)) of 0.10+/-0.01 (SEM)x10(-3)cm/min. Fourier transform infrared ...
Mesenchymal stem cells (MSCs) are increasingly used as therapeutic agents as well as research tools in regenerative medicine. Development of technologies which allow storing and banking of MSC with minimal loss of cell viability, differentiation capacity, and function is required for clinical and research applications. Cryopreservation is the most effective way to preserve cells long term, but it involves potentially cytotoxic compounds and processing steps. Here, we investigate the effect of decreasing dimethyl sulfoxide (DMSO) concentrations in cryosolution by substituting with hydroxyethyl starch (HES) of different molecular weights using different freezing rates. Post-thaw viability, phenotype and osteogenic differentiation capacity of MSCs were analysed. The study confirms that, for rat MSC, cryopreservation effects need to be assessed some time after, rather than immediately after thawing. MSCs cryopreserved with HES maintain their characteristic cell surface marker expression as well as the
Attain higher post-thaw sperm recovery with the only CE marked and FDA approved medium with TEST-yolk buffer (TYB) and glycerol for freezing sperm.
I15 Should Cryoprotectants be Formulated According to the Mouse Genome?. Mike Legge, Mat Byers and Stephen Bird. Molecular Embryology Group, Department of Biochemistry, University of Otago, PO BOX 56 Dunedin, New Zealand. The use of penetrating cryporotectants such as dimethyl sulphoxide and I,2-propanediol is well established for mouse embryo cryopreservation. However, the sensitivity of both oocytes and embryos to adverse effects of both cryoprotectants and the freezing process is well documented (Duliquost et al. 1999). For mouse genome cryopreservation this is especially important as there is a correlation between mouse embryo genotype and cryopreservation success. (Schmidt et al. 1985). With the increasing necessity to archive mouse genomes by embryo and gamete cryopreservation the improvement in cryoprotectant formulations is becoming increasingly important. Recently we identified the non-enzymatic formation of formaldehyde in cryoprotectant solutions and that at the micro-molar ...
Mouse half ovaries were cryopreserved and orthotopically transplanted into ovariectomized recipients genetically identical to ovary donors except for the coat color gene. Fertility was reestablished in 57% of the female recipients, which became pregnant in an average of 40 days after transplantation of frozen-thawed half ovaries. These experiments demonstrate that ovary cryopreservation can be a very useful option for banking mouse germplasm, or managing subfertile animal colonies, when embryo or sperm freezing cannot be used or is not cost effective.
Cryopreservation of in vivo derived Jersey bovine embryos have resulted in a 10% lower pregnancy rate compared to other dairy breeds. Poor embryo survival after cryopreservation has been partially attributed to the high lipid content of Jersey embryos. In vitro-produced (IVP) bovine embryos have darker cytoplasm than their in vivo-derived counterparts because of higher lipid accumulation. High lipid accumulation is associated with impaired embryo quality. Forskolin is an adenylate cyclase activator that regulates cAMP levels in cells and has been shown to induce lipolysis in IVP embryos. L-carnitine is required for transport of fatty acids from the intermembrane space of the mitochondria into the mitochondrial matrix to support the process of β-oxidation, and enhances ATP production. We hypothesized that the lipid content of in vivo-produced and IVP Jersey embryos is higher than respective Holstein embryos and that forskolin + L-carnitine would reduce lipid content of IVP embryos and vitrification with
It is well known that laser-assisted hatching (LAH) is the most popular and ideal embryo hatching technology, but the relevance to pregnancy outcomes of cryopreserved-thawed embryo transfer (ET) is...
Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request ...
To provide a novel fertility preservation option for patients facing a fertility threatening cancer diagnosis or treatment regimen by establishing an ovarian tissue cryopreservation program. To determine if ovarian tissue cryopreservation provides women with a useful, successful option for fertility preservation. The hypothesis is that ovarian tissue cryopreservation for fertility preservation provides an alternative option for fertility preservation. ...
BACKGROUND: Cryopreserved human sperm are used in assisted reproductive technology. However, the effect of cryopreservation on sperm DNA integrity is unclear. OBJECTIVES: The objectives of this study were to: (i) determine the impact of semen cryopreservation on human sperm DNA integrity and chromatin structure; (ii) test if parameters obtained from TUNEL and SCSA® correlate; and (iii) verify correlation between sperm motility, morphology and viability with TUNEL and SCSA® parameters. MATERIALS AND METHODS: Men attending a fertility clinic were recruited and grouped according to their sperm parameters (n = 9/group): normozoospermia, oligoasthenoteratozoospermia and teratozoospermia. Each semen sample was processed as follow: (i) directly frozen at -80 °C; (ii) diluted in Sperm Maintenance Medium, cooled for 30 min at 4 °C and frozen at -80 °C; (iii) diluted in Sperm Maintenance Medium; or (iv) in SpermFreeze. Each mixture from method (iii) and (iv) was then suspended for 30 min in liquid nitrogen
Cryopreservation is the preservation of cells, tissues or even organs at very low temperatures, with the intention of future use. At the temperature of -196oC, metabolism stops and the cells enter a state of suspended animation making it possible to store them for a long time. To achieve such a low temperature we use liquid nitrogen. The first successful cryopreservation was performed in frogs in 1945. The first animal sperm banks operated in 1949. The first successful inseminations I the human were reported in 1953 (preservation in dry ice, -70oC) and in 1964 in liquid nitrogen. Embryo cryopreservation was first performed in laboratory animals in 1972 and in human in 1984.. Today, cryopreservation is a routine method, allowing the storage of gametes and embryos for an extended period.. ...
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Whenever a sudden shift is made from fresh embryo transfer to frozen embryo transfer the couple usually have many queries in mind. So, lets evaluate both and see pros and cons.. Fresh embryos transfer is undergoing ovarian hyper stimulation making of embryos and transferring resultant embryos to uterine cavity in same cycle. In frozen embryo transfer first two steps of controlled ovarian hyper stimulation and process of IVF/ICSI are same but the resultant embryos are frozen using vitrification techniques and not transferred in same cycle. They are thawed and then kept uterine cavity after preparing the uterine cavity. In patients who are normal or hyper responders the frozen embryo transfers are known to have better results than the fresh embryo transfer . The reason can be that due to ovarian hyper stimulation and high levels of estrogen may have negative impacts on the endometrial receptivity. In some cycles another hormone called progesterone may also be raised, which again decreases embryo ...
TY - JOUR. T1 - The bioenergetics of mitochondria after cryopreservation. AU - Fuller, Barry J.. AU - Rubinacci, Alessandro. AU - Geboes, Karel. AU - De Loecker, William. PY - 1989. Y1 - 1989. N2 - The functional characteristics of rat liver mitochondria after cryopreservation with and without the addition of the cryoprotectant dimethyl sulfoxide (Me2SO) were evaluated. As criteria of functional integrity, polarographic measurements of substrate-linked oxygen consumption and luminescent assay of adenosine triphosphate (ATP) synthesis were considered before and after cryopreservation. The results demonstrated that mitochondrial damage after freezing was indicated by the polarographic studies but was not evident when ATP synthesis was considered. Me2SO present during cryopreservation was partially protective for mitochondrial substrate-linked oxygen consumption; however, simple exposure to and dilution from Me2SO effected some changes in mitochondrial function.. AB - The functional characteristics ...
Sometimes couples undergoing in vitro fertilization (IVF) need more than a single cycle to conceive. In recent years, frozen embryo transfers (FET) has become a popular option before moving to a fresh IVF cycle. FET at PRC allows you to extend the chance of pregnancy per egg retrieval, saving you time and money.. FROZEN EMBRYO TRANSFER (FET). A frozen embryo transfer (FET) is a cycle in which the frozen embryos from a previous fresh IVF or donor egg cycle are thawed and then transferred back into the womans uterus.. Lower Cost. The cost of frozen egg transfer is significantly lesser than a fresh cycle of IVF. This combination of reduced cost and equal success rates makes frozen embryo transfers an exciting option over fresh IVF cycle at the top IVF & egg fertility clinic in Los Angeles.. Less Medication. In a fresh cycle, you would again need to take stimulation medication. On the other hand FET IVF & egg fertility clinic in Los Angeles just requires you to use estrogen and progesterone to ...
A cohort of 91 children from cryopreserved embryos and 83 control children who were conceived normally had their development assessed using the Griffithss scales of mental development. The controls (81 singletons and two twins) of a similar age, sex, and social class were selected from siblings, cousins, and peers of the cryopreserved embryo group (68 singleton, 20 twins, and three triplets). Children from cryopreserved embryos had a lower mean birth weight and mean gestational age and a higher proportion were born by caesarean section. One child from the cryopreserved embryo group had Downs syndrome, three had squints, and four had conductive hearing loss while in the control children, six had squints, and nine had conductive hearing loss. In both groups, including the child with Downs syndrome, the mean Griffithss quotient was greater than the standard 100. In the children from cryopreserved embryos, the singleton and multiple birth subgroups had statistically similar assessment results. ...
TY - JOUR. T1 - Markers of growth and development in primate primordial follicles are preserved after slow cryopreservation. AU - Jin, Shiying. AU - Lei, Lei. AU - Shea, Lonnie D.. AU - Zelinski, Mary B.. AU - Stouffer, Richard L.. AU - Woodruff, Teresa K.. N1 - Funding Information: Supported by Oncofertility Consortium: National Institutes of Health grants RL1-HD058295 and PL1EB008542 and Training for a New Interdisciplinary Research Workforce (T90) grants 1TL1CA133837 , U54-HD18185 , and NCRR RR00163 . PY - 2010/5/15. Y1 - 2010/5/15. N2 - Objective: To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices. Design: Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters. Setting: University-affiliated laboratory. Animals: Rhesus monkey ovarian tissue. Intervention(s): None. Main Outcome Measure(s): Histologic analysis, follicle counting, ...
BACKGROUND: Vanilla siamensis is listed in Appendix-II of Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) as an endangered species in Thailand. OBJECTIVE: To develop an optimum cryopreservation protocol for V. siamensis. MATERIALS AND METHODS: Protocorms were precultured on solid ½ MS medium with 0.5 M sucrose for 0-7 d. For encapsulation-dehydration, encapsulated protocorms (beads) were dehydrated for 0-6 h. In the case of encapsulation-vitrification, the beads were loaded with a plant vitrification solution 2 (PVS2) at 0°C for 0-90 min. RESULTS: Protocorms precultured for 3 d gave the highest post-cryopreservation survival of 17%. Dehydration of the encapsulated protocorm beads for 4 h gave the highest survival of 33% and a regrowth of 25%. Protocorms subjected to the encapsulation- vitrification method did not survive at all. CONCLUSION: Protocorms precultured with 0.5 M sucrose for 3 d, encapsulated with 3% sodium alginate and dehydrated to a ...
According to a recent report from the HFEA features a pattern towards Assisted Reproductive Technology (ART) enlistments across the globe. It states that frozen embryo transfer has been increasingly seen as a mainstream embryo transfer protocol.. HFEA measurements depict that fresh embryo transfers dropped by 11 percent somewhere in the range of 2013 to 2018. On the other hand, frozen embryo transfers nearly multiplied, representing 38% of all In Vitro Fertilization (IVF) cycles in 2018.. At one center, frozen embryo banks outperformed intercontinental donations as a treatment for donors from upcoming years after 2014. Hence, of the 1283 donor cycles finished somewhere in the range of 2005 to 2013. Out of these, 88 percent were treated as treatments from other states, yet today 95% of egg donors have moved to frozen donor eggs, in egg bank.. Also, the pattern of blastocyst transmission in some centers mirrors a similar trend now, that is currently clear in the developing enlistment information: ...
Both proceedures have seen great success rates in the last five years and thousands of baby boys and girls have been born using this technology.. Endocrine Abstracts says, Semen cryopreservation is a viable fertility preservation option for adolescent cancer patients, and should be offered to all before treatment, acknowledging the caveat of a ~50% chance of success. Pubertal staging is the only significant prognosticator of this and should be routinely assessed as part of the counselling process.. While the ethics surrounding fertility preservation in younger cancer patients are being debated, research is ongoing to take fertility tissue from patients where eggs and sperm are not necessarily matured or viable.. Speak to your health professional about fertility preservation options they may be aware of. The two of you will be able to decide if the process is right for you and decide on your next steps.. If youre wary of fertility options, remember, Geoff has managed to father two beautiful ...
Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different diatom species, representing the major morphological and ecological diatom groups, using a range of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the post-thaw recovery process. Our results show that the effects of cryoprotectant choice, its concentration and the ...
There is a lump sum due at the time of cryopreservation. This pays for the cost of transportation, cryopreservation protocols, and also for ongoing costs of maintenance for the cryonics patient. This lump sum is normally covered with a dedicated life insurance policy.. Cryonics organizations also have membership dues, which are separate from the cost of the cryonics life insurance. There are no additional costs due once a member is in cryopreservation.. ...
Cryopreservation is a reliable means for the long-term conservation of plant genetic resources. It is of particular interest for cocoa (Theobroma cacao L.) whose seeds are recalcitrant to conventional storage methods and field collections susceptible to disease infestations. However, the encapsulationdehydration procedure previously developed for cocoa somatic embryos has resulted in poor survival after retrieval from liquid nitrogen. To examine the causes of such failure, cocoa somatic embryos following each treatment step of the encapsulation-dehydration procedure were examined using a combination of confocal scanning laser microscopy and transmission electron microscopy. Results showed that the parenchymas cells of the hypocotyl and radicle were the major sites of injury possibly due to their large size and non-cytoplasmic nature, whereas the shoot meristem and provascular strand were well preserved throughout the treatments. In general, cell deformation and/or disruption was observed following the
The summer flounder, Paralichthys dentatus L., is a high-value species and considerable research has been conducted to determine practices conducive for its culture. As milt can be limited in this species, experiments were conducted to develop a practical sperm cryopreservation protocol for hatchery use. Two dilution ratios (1:2 and 1:4; sperm:extender), 2 diluents (saline and sucrose-based), 2 cryoprotectants (10% DMSO and 12% glycerol) and 3 freezing rates (?5, ?10 and ?15°C min?1) were evaluated using differential staining to assess post-thaw sperm survival. Seven combinations of the factors examined reduced post-thaw viability by less than 30%. The average viability of sperm from fresh, pooled flounder milt (67.2 ± 2.9%) was not different from that of thawed milt diluted 1:4 with sucrose diluent (10% DMSO) frozen at ?5°C min?1 (38.4 ± 7.7%) and fertilization and hatch success were not different in trials using fresh or thawed, cryopreserved sperm. From these experiments a practical
Principles of Cryopreservation and Optimization of Vitrification This talk discusses about the principles of using cryoprotectants to achieve cryopreservation including slow freezing and vitrification. Based on the principles, several approaches used to improve vitrification are reviewed. The future directions of the c
Despite of important advances in the knowledge of the reproductive biology of several deer species (mainly, red deer), there is a lot to investigate. We are trying to answer a practical and a basic question: how can we improve sperm cryopreservation in cervids? and, which underlying, cellular and molecular, changes occur during cooling and cryopreservation?. These are important questions not only for our Iberian deer species (Iberian red deer and roe deer), but also to endangered cervids elsewhere in the world (specially South-American species such as the huemul and pudu). Technology transfer from well-known species to related but less studied species might improve their conservation.. ...
TY - JOUR. T1 - Ovine semen cryopreservation usingthree extenders and four combinations of permeant and non permeantagents. AU - Rocío Sandoval, M.. AU - Alexei Santiani, A.. AU - Luis Ruiz, G.. AU - Víctor Leyva, V.. AU - Luis Coronado, S.. AU - Alfredo Delgado, C.. PY - 2007/1/1. Y1 - 2007/1/1. N2 - © 2007 Universidad Nacional Mayor de San Marcos. All rights reserved. The objective of the study was to evaluate the effect of three extenders and four combinations of two permeant and two non permeant cryoprotectant agents on the quality of post thaw ram semen. In Experiment 1, three extender were evaluated (A, B, and C) in order to select the best for the next step. In Experiment 2, different combinations of cryoprotectant agents were evaluated as follow: 1) Glycerol-Trehalose, 2) Glycerol- Sucrose, 3) Ethylene glycol-Trehalose, and 4) Ethylene glycol-Sucrose. In experiment 1, motility, viability and acrosomal integrity in extender A were higher than in extender B and extender C, and ...
IVF and ICSI are fully established methods of assisted reproduction which can be used for patients awaiting cytotoxic therapy:. Following the data of the German, Swiss and Austrian network Fertiprotekt (www.fertiprotekt.eu), 164 of 1388 counselled patients have chosen ovarian stimulation and cryopreservation of oocytes as a fertility preservation technique in 2007 2009. Among those patients 2417 oocytes were collected (Mean: 11.8, Range 0 41, STD 7.3). In 125 patients oocytes were fertilized and cryopreserved, resulting in an fertilisation rate of 70.5%/aspirated oocyte. Only in one patient, chemotherapy needed to be postponed due to severe ovarian hyperstimulation syndrome.. These data reveal that ovarian stimulation can result in adequate numbers of oocytes. However, they also demonstrate, that this technique is not successful in all patients. Combination with cryopreservation of ovarian tissue should therefore be considered. FertiPROTEKT performed a study in which 50% of one ovary was removed ...
Guidelines contents   Introduction   Recommendations   Discussing fertility   Management   Impact   Options  
Poster (2015, June 14). Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results ... [more ▼]. Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results obtained with HGL5 granulosa cell line suggest that Z-VAD-FMK is efficient to protect granulosa cells from etoposide or CoCl2 induced apoptosis. What is known already: Removal, cryopreservation and subsequent graft of ovarian strips after cancer treatment have been successfully used to re-establish female fertility. However, the pregnancy rate after autografting of cryopreserved tissue is about 30%. Indeed, the major problem after transplantation is follicular loss due to ischemic reperfusion injury. Study design, size, duration: Three human granulosa cell lines (GC1a, ...
TY - JOUR. T1 - Sucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservation. AU - Coticchio, G.. AU - De Santis, L.. AU - Rossi, G.. AU - Borini, A.. AU - Albertini, D.. AU - Scaravelli, G.. AU - Alecci, C.. AU - Bianchi, V.. AU - Nottola, S.. AU - Cecconi, S.. PY - 2006/7. Y1 - 2006/7. N2 - Background: Recently described slow-cooling cryopreservation protocols involving elevated sucrose concentration have improved survival frequencies of human oocytes, potentially overcoming a major hurdle that has limited the adoption of oocyte storage. Because implantation rates of embryos from frozen oocytes remain generally low, it is still debated whether, irrespective of survival rates, this form of cryopreservation leads inevitably to the disruption or complete loss of the metaphase II (MII) spindle. Methods: Human oocytes with an extruded polar body I (PBI) were cryopreserved using a slow-cooling method including 1.5 ...
This three-days training course is specifically designed for Embryologists interested in acquiring practical skills and updating their knowledge on oocyte, embryo, and blastocyst cryopreservation techniques. The course will focus on didactic and intensive hands-on practical lessons on the vitrification system and media that the embryologist needs to implement, pointing out the tips and tricks to achieve consistent results. Throughout the three-days, embryologists will have the opportunity to vitrify and warm unlimited number of oocytes and embryos needed to understand how important little details can affect results. The course also covers the fundamental principles of the assisted hatching procedure and its multiple applications in different developmental stages. Introduction to the Laser Assisted Hatching and familiarization with different operational modes. Evaluation and discussion about the effect of the size of the hole on the developing embryo by culturing treated embryos in a TL ...
By Leigh E. Towill (auth.), Dr. Leigh E. Towill, Prof. Dr. Y. P. S. Bajaj (eds.). Ex situ upkeep of germplasm for better plant species has been accom- plished utilizing both seeds or clones, yet garage of those less than commonplace condi- tions doesnt give you the severe longevities which are had to reduce danger of loss. expenditures of upkeep and regeneration of shares also are excessive. platforms that supply almost indefinite garage should still complement current tools and its inside this context that cryopreservation is gifted. using low temperature upkeep was once firstly extra a priority of drugs and animal breeding, and used to be multiplied to crops within the Nineteen Seventies. Sur- vival after cryogenic publicity has now been validated for various plant teams together with algae, bryophytes, fungi and better vegetation. If survival is com- monplace, then the eventual program is a cryopreservation process, wherein cells, tissues and organs are held indefinitely to be used, usually ...
The effect of cryopreservation and long-term liquid nitrogen storage on peripheral blood mononuclear cell (PBMC) subsets was prospectively analyzed using monoclonal antibodies and flow cytometry. Brief cryopreservation did not significantly alter the proportion of positively stained cells for CD3+, CD4+, CD8+, CD14+, CD16+, and CD19+ cells. A small but statistically
Research from many laboratories over the past several decades indicates that invertebrate oocytes and eggs are extraordinarily difficult to freeze. Since starfish oocytes, eggs, and embryos are an important cell and developmental biology model system, there is great interest to cryopreserve these cells. Previous starfish oocyte cryopreservation studies using slow cooling protocols revealed that these cells are highly sensitive to osmotic stress and form intracellular ice at very high sub-zero temperatures, suggesting that common freezing methodologies may not prove useful. We report here that a short exposure to 1.5 M Me2SO/1 M trehalose in hypotonic salt solution followed by ultra-rapid cooling to cryogenic temperatures allows starfish oocytes to be cryopreserved with the average survival rate of 34% when normalized to control oocytes that were exposed to CPA, but not frozen. On average, 51% of the oocytes in 77% of the batches of frozen oocytes underwent meiotic maturation in response to the ...
Since the discovery of Vitrification in 2008, the pregnancy rate of frozen embryos is exactly the same or, in some cases, higher than that of fresh embryos transferred after In Vitro Fertilisation. Pregnancy rates are five times higher than the gestation rates of intrauterine insemination, which is why frozen embryo transfer is currently one of the most effective and safest techniques to welcome a baby home.. Frozen embryo transfer is the most effective and safest technique if we want to achieve a full-term pregnancy in cases of infertility or desire to be a mother. Pregnancy rates are related to the age of the couple, especially the mother, since it clearly accentuates the possibility of gestation.. ...
Egg freezing and cryopreservation services provided by Dr. Akin at Bluegrass Fertility Center located in Lexington, KY. Other services include IVF, Assisted Hatching, ICSI and PGD.
Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to Show moreCryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to assess the ...
The delivery rate achieved after the vitrification of early cleavage- and blastocyst-stage embryos is not affected by the embryo developmental stage or any other variable related to the warming cycle.
Frozen embryo transfers have higher success rates than fresh embryo transfers. Within frozen embryo transfers there are natural and programmed cycles. Each have tradeoffs in terms of cost, intensity, drug requirements and more.
Results of this study10 were surprising, to say the least. Without going into the specifics of different types of fertility preservation and their respective indications, less than half of the respondents (45.6%) reported being aware of fertility preservation. Specialists in O&G fared no better in this regard with only half (50.7%) of the respondents reporting themselves as being aware. As expected, O&G specialists were more aware of fertility-preservation techniques in females such as oocyte- and embryo-freezing as well as ovarian tissue freezing, than their non-O&G counterparts. Interestingly, when respondents were further asked about individual fertility-preservation procedures, an increased awareness was found. In fact, a higher percentage of the same O&G specialists in this study reported to be familiar with all of the above fertility-preservation techniques previously itemised, compared with being aware of fertility preservation per se (63.6% vs 50.7%). These findings highlight a ...
Ovarian tissue cryopreservation (OTC) is offered to women treated for acute leukemia to preserve their fertility before hematopoietic stem cell transplantation. The risk of leukemic infiltration in ovarian samples harvested before administration of chemotherapy limits ovarian tissue transplantations. We assessed the minimal residual disease (MRD) by sensitive quantitative polymerase chain reaction in cryopreserved ovarian cortex and medulla samples harvested from 30 patients in complete remission of acute leukemia, including 60 % with negative bone marrow MRD at the time of OTC. Ovarian MRD was undetectable in 21 patients (70%), detectable below 10-4 in 8 patients (27%) and between 10-3 and 10-4 in 1 patient (3%). Twenty patients (67%) had concordant MRD between bone marrow and ovarian samples. Interestingly 4 patients had positive MRD in ovarian samples while undetectable in bone marrow. Our results underline the importance of reaching the best control of the disease with undetectable or low ...
Mary Zelinski, PhD finishes her reports from the annual meeting of the Society for Cryobiology held from Corvallis, Oregon with a final blog about the keynote
Knowing that there are options for many women to protect or preserve their reproductive potential before cancer treatment begins can make it less challenging to discuss the possible reproductive side effects of treatment with your patients
TY - JOUR. T1 - Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. AU - Teo, Ka Yaw. AU - DeHoyos, Tenok O.. AU - Dutton, J. Craig. AU - Grinnell, Frederick. AU - Han, Bumsoo. N1 - Funding Information: This research was supported by grants from the National Institute of Health / National Institute of Biomedical Imaging and Bioengineering , R01 EB008388 , and the National Science Foundation , CBET-1009465 . PY - 2011/8. Y1 - 2011/8. N2 - The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In ...
Not all frozen embryo transfer cycles and protocols are the same; however, I am sharing my FET cycle to provide insight into how FET cycles can go.
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The purpose of this study was to test of efficacy of melatonin on the Optimizing of cryopreservation media in the testis tissue samples. Testes from neonate BALB/c mice were vitrified and then thawed under standard condition with or without the addition of 100 μM melatonin to both of vitrification and thawing solution. After that, Vitrified-thawed whole testes were digested under standard condition and subsequent viability of the cells in the suspension was analyzed using cytotoxicity kit and Apo-Brdu tunnel assay kit. The mean proportion of apoptotic testicular cells in the treated vitrified-thawed testes in comparison to no-treated ones was noted significantly (5.2±0.47 vs. 1.56±0.62, respectively). Moreover, melatonin cause decreasing the viability of the treated vitrified-thawed testicular cells in compared to no-treated vitrified-thawed testicular cells (4.78±0.46 vs. 8.39±0.76, respectively). In addition, the mean cytotoxicity of melatonin on the vitrified-thawed testicular cells was ...
What are benefits of frozen embryo transfer? Before the advent of embryo freezing , surplus embryos had to be discarded after fresh embryo transfer. Read on to find out more
PubMed journal article: Successful elective and medically indicated oocyte vitrification and warming for autologous in vitro fertilization, with predicted birth probabilities for fertility preservation according to number of cryopreserved oocytes and age at retrieval. Download Prime PubMed App to iPhone, iPad, or Android
FERRARI, Edilene Aparecida Preti; COLOMBO, Ronan Carlos; FARIA, Ricardo Tadeu de and TAKANE, Roberto Jun. Criopreservação de sementes de Encholirium spectabile Martius ex Schultes f. pelo método da vitrificação. Rev. Ciênc. Agron. [online]. 2016, vol.47, n.1, pp.172-177. ISSN 0045-6888. http://dx.doi.org/10.5935/1806-6690.20160020.. A bromélia Encholirium spectabile Martius ex Schultes f. é uma espécie endêmica da Caatinga. O objetivo deste trabalho foi avaliar a eficiência de soluções crioprotetoras em sementes de bromélia. Os tratamentos consistiram da imersão das sementes em soluções crioprotetoras e de vitrificação, antes da imersão em nitrogênio líquido (NL) (-196 ºC), conforme os tratamentos a seguir: T1 - controle: sem crioprotetores; T2 - glicerol 2M (20 min) + PVS2 (10 min); T3 - glicerol 2M (20 min) + PVS2 com floroglucinol a 1% (10 min); T4 - sacarose 0,4M (20 min) + PVS2 (10 min); T5 - sacarose 0,4M (20 min) + PVS2 com floroglucinol a 1% (10 min); T6 - glicerol ...
The Fertility Preservation Program also will endeavor to aid women and girls who are starting cancer treatment or preparing for a bone marrow transplant. Currently their choices are quite limited and, for many, biologically impossible or morally unacceptable, noted Joseph S. Sanfilippo, M.D., director of the Center for Fertility and Reproductive Endocrinology at Magee-Womens Hospital.. Women can undergo ovarian stimulation to generate multiple mature eggs, which can then be fertilized with partner or donor sperm to produce embryos for later implantation and possible pregnancy, he said. These techniques are now well-established, and we will offer them to female patients who wish to pursue this option. Another option is ovarian cryopreservation, where an ovary is removed before cancer therapy is initiated with the plan to replace ovarian tissue once the patient is cured.. Still, the stimulation technique takes time and could delay cancer treatment. It also produces high estrogen levels that ...
Cryopreservation[edit]. Cryopreservation of ovarian tissue, often called ovarian tissue cryopreservation, is of interest to ... Oktay K, Oktem O (November 2008). "Ovarian cryopreservation and transplantation for fertility preservation for medical ...
Chian, Ri-Cheng (2010). Fertility Cryopreservation. Cambridge University Press. p. 52. ISBN 9780521517782. v t e. ...
Cryopreservation was applied to human cells beginning in 1954 with frozen sperm, which was thawed and used to inseminate three ... Cryopreservation may be accomplished by freezing, freezing with cryoprotectant to reduce ice damage, or by vitrification to ... In London in 2016, the English High Court ruled in favor of a mother's right to seek cryopreservation of her terminally ill 14- ... In the initial cryopreservation protocol, the subject is intubated and mechanically ventilated, and a highly efficient ...
He was also Head of the Tissue Cryopreservation Section of the Transfusion and Cryopreservation Research Program of the U.S. ... More recently, he received the Cryopreservation Award from the International Longevity and Cryopreservation Summit held in ... Fahy has over thirty years of experience in the field of cryopreservation. As a scientist with the American Red Cross, he was ... Fahy GM, Wowk B, Wu J, Phan J, Rasch C, Chang A, Zendejas E (2004). "Cryopreservation of organs by vitrification: perspectives ...
Pegg, DE (2007). "Principles of Cryopreservation". In Day JG, Stacey GN (eds.). Cryopreservation and Freeze-Drying Protocols. ... Cryopreservation and Freeze-Drying Protocols, Second Edition. Methods in Molecular Biology. 368. Humana Press. pp. 39-57. doi: ...
"Cryopreservation of monocots". Plant Cryopreservation: A Practical Guide. pp. 241-280. doi:10.1007/978-0-387-72276-4_11. ISBN ... 2008). Plant cryopreservation a practical guide. New York: Springer. ISBN 978-0-387-72276-4. Short, Emma; George, Alex (2013). ...
Abazari A, Jomha NM, Elliott JA, McGann LE (2013). "Cryopreservation of articular cartilage". Cryobiology. 66 (3): 201-209. doi ...
Chen, C (April 1986). "Pregnancy after human oocyte cryopreservation". Lancet. 1 (8486): 884-6. doi:10.1016/S0140-6736(86)90989 ... Gook, Debra A. (1 September 2011). "History of oocyte cryopreservation". Reproductive BioMedicine Online. 23 (3): 281-289. doi: ...
It is also commonly used in cryopreservation of blood vessels along with the other glycosaminoglycans and protein suspensions. ... Müller-Schweinitzer E, Hasse J, Swoboda L (1993). "Cryopreservation of human bronchi". J Asthma. 30 (6): 451-7. doi:10.3109/ ... Müller-Schweinitzer E, Ellis P (May 1992). "Sucrose promotes the functional activity of blood vessels after cryopreservation in ... Brockbank KG (February 1994). "Effects of cryopreservation upon vein function in vivo". Cryobiology. 31 (1): 71-81. doi:10.1006 ...
CryopreservationEdit. Main articles: Oocyte cryopreservation and Embryo cryopreservation. Cryopreservation can be performed as ... Also, oocyte cryopreservation can be used for women who are likely to lose their ovarian reserve due to undergoing chemotherapy ... Typically, genetic parents donate the eggs to a fertility clinic or where they are preserved by oocyte cryopreservation or ... By 2017, many centers have adopted embryo cryopreservation as their primary IVF therapy, and perform few or no fresh embryo ...
This work is focused on improving the process of cryopreservation itself, e.g., to improve vitrification technology, or to ... Almost as many as 200 Russian citizens have entered into cryopreservation agreements with the company. It is thought that ... Prior to the companys' founding, its prospective staff members have already had some experience in cryopreservation, when in ... This is being justified as follows: "Activities, directed towards cryopreservation of people can be considered fraud if an ...
Henkelman S, Lagerberg JW, Graaff R, Rakhorst G, Van Oeveren W (November 2010). "The effects of cryopreservation on red blood ... These techniques can be used to assess the success of cell culture techniques, cryopreservation techniques, the toxicity of ... Pichugin Y, Fahy GM, Morin R (April 2006). "Cryopreservation of rat hippocampal slices by vitrification". Cryobiology. 52 (2): ... Crutchfield A, Diller K, Brand J (1999-02-01). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal ...
He did important translational research on ovarian tissue cryopreservation and transplantation. Gosden was born on 23 September ... Prasath, Ethiraj B. (1 January 2008). "Ovarian tissue cryopreservation: An update". Journal of Human Reproductive Sciences. 1 ( ... Silber, S. J. (1 February 2012). "Ovary cryopreservation and transplantation for fertility preservation". Molecular Human ... and he did important translational research on ovarian tissue cryopreservation and on ovary transplantation; his interests also ...
Donor blood cryopreservation also reduces the number of times a donor needs to have blood drawn for cross matching, making ... The implementation of the cryopreservation service allowed NKR centers to dramatically reduce the time to complete a cross ... "National Kidney Registry Initiates Donor Blood Cryo-Preservation" (Press release). National Kidney Registry. December 2, 2014. ... Berz; McCormack; Winer; Colvin; Quesenberry (November 12, 2007). "Cryopreservation of Hematopoietic Stem Cells". Am. J. Hematol ...
Porcu, Eleonora; Ciotti, Patrizia; Venturoli, Stefano (2012-12-06). Handbook of Human Oocyte Cryopreservation. Cambridge ... useful for applications such as sperm cryopreservation. The semi-solid mixture can also be called slush nitrogen or SN2. Solid ... in liquid and slush nitrogen by numerical simulation of cooling rates for French straws used for sperm cryopreservation". ...
2005) Biology of Plants, 7th ed., page 459 Reed, Barbara (2008). Plant cryopreservation a practical guide. New York: Springer. ...
The use of cryopreservation agents is also key to the freezing process. A common cryoprotection agent used is 10% solution of ... Coopman, K (2013). Cryopreservation: Technologies, Applications and Risks/Outcomes (PDF). Nova Science Publishers. pp. 91-108. ... Rapid thaws are recommend in bringing the cells out of cryopreservation and starting up their normal metabolic processes. ... Cell banks are commonly used within fields including stem cell research and pharmaceuticals, with cryopreservation being the ...
These methods include: Cryopreservation Mummification; the most well-known examples are from ancient Egypt Taxidermy; an ...
In 1983, Alan Trounson and Linda Mohr reported the first pregnancy which used embryo cryopreservation (frozen human embryos). ... Christopher Chen reported the first pregnancy which used oocyte cryopreservation (frozen eggs). The ability to freeze sperm has ... Chen, Christopher (1986-04-19). "PREGNANCY AFTER HUMAN OOCYTE CRYOPRESERVATION". The Lancet. Originally published as Volume 1, ...
Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...
Cryopreservation can be performed as oocyte cryopreservation before fertilisation, or as embryo cryopreservation after ... Semen cryopreservation Evans v United Kingdom, a key case at the European Court of Human Rights Sex selection Stem cell ... Also, oocyte cryopreservation can be used for women who are likely to lose their ovarian reserve due to undergoing chemotherapy ... By 2017, many centers have adopted embryo cryopreservation as their primary IVF therapy, and perform few or no fresh embryo ...
"UK Cryonics and Cryopreservation Research Network". Retrieved 2 February 2016. Chibber, Kabir (11 May 2015). "Meet the people ... by signing an open letter to support research into cryonics and by being an advisor to the UK Cryonics and Cryopreservation ...
He has advocated human cryopreservation, for example by signing an open letter to support research into cryonics, being a ... "UK Cryonics and Cryopreservation Research Network". Retrieved 2016-02-02. Benford, Gregory (1992-08-01) [1980]. Timescape. ... member of Alcor, and by being an advisor to a UK cryonics and cryopreservation advocacy group. Gregory Benford retired from the ...
"A ferromagnetic model for the action of electric and magnetic fields in cryopreservation". Cryobiology. 68 (2): 163-165. doi: ... "Electric and magnetic fields in cryopreservation". Cryobiology. 64 (3): 301-3, author reply 304-5. doi:10.1016/j.cryobiol. ...
Storage by cryopreservation, on the other hand, will be in the −80 to −196 °C temperature range. Organs, and tissues are more ... Cryopreservation of cells is guided by the "two-factor hypothesis" of American cryobiologist Peter Mazur, which states that ... Cryopreservation in humans with regards to infertility involves preservation of embryos, sperm, or oocytes via freezing. ... This led to a much wider use of cryopreservation today, with many organs, tissues and cells routinely stored at low ...
Cryopreservation and culture tissue after cryopreservation. Cryopreservation of ovarian tissue is of interest to women who want ... Oktay K, Oktem O (February 2010). "Ovarian cryopreservation and transplantation for fertility preservation for medical ...
Racharaks, Ratanachat; Peccia, Jordan (2019). "Cryopreservation of Synechococcus elongatus UTEX 2973". Journal of Applied ...
... s are found in some science fiction films such as Prometheus (2012) and The Host (2013). Cryopreservation Kopenkoskey, ...
9 February - A breakthrough in cryopreservation is announced, with a rabbit's whole brain shown to have a well-preserved ... "New cryopreservation procedure wins Brain Preservation Prize". KurzweilAI. 9 February 2016. Retrieved 11 February 2016. " ...
They used cryopreservation of gametes and embryos. Using cryopreservation and recent cloning technologies are considerations ...
Main article: Cryopreservation of testicular tissue. Cryopreservation of immature testicular tissue is a developing method to ... Main article: Oocyte cryopreservation. Human oocyte cryopreservation is a new technology in which a womans eggs (oocytes) are ... Perry SF (1995). "Freeze-drying and cryopreservation of bacteria". Cryopreservation and Freeze-Drying Protocols. Methods in ... Main article: Ovarian tissue cryopreservation. Cryopreservation of ovarian tissue is of interest to women who want to preserve ...
Cryopreservation is based on the ability of certain small molecules to enter cells and prevent dehydration and ice crystal ... Cryopreservation, the preservation of cells and tissue by freezing. ... Cryopreservation. Discuss Print Cite verifiedCite While every effort has been made to follow citation style rules, there may be ... Cryopreservation is also used to freeze and store human embryos and sperm. It is especially valuable for the freezing of extra ...
... ABSTRACT: In 2013, the American Society for Reproductive Medicine and the Society for Assisted ... Oktay K, Cil AP, Bang H. Efficiency of oocyte cryopreservation: a meta-analysis. Fertil Steril 2006;86:70-80. [PubMed] [Full ... Human oocyte cryopreservation. Hum Reprod Update 2007;13:591-605. [PubMed] [Full Text] ⇦ ... Mature oocyte cryopreservation is a currently available method of fertility preservation in women of reproductive age. Although ...
In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ... Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in ... Cryopreservation Tools for Microbiology ATCC has the tools you need for the successful cryopreservation of your microbial ... Cryopreservation Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires ...
Embryo cryopreservation is an efficient, reliable, and cost-effective means of safeguarding and maintaining stocks of ... One week before the scheduled cryopreservation date, pick up 1 tube of M2 media, 1 tube of PMSG, and 1 tube of HCG from the ... HCG is given 47 hours after PMS at 9 a.m., one day before the scheduled rederivation date (e.g. Thursday for cryopreservation ... Embryo cryopreservation is an efficient, reliable, and cost-effective means of safeguarding and maintaining stocks of ...
"Here, a paper‐based cell cryopreservation method as an alternative to conventional cryopreservation methods is presented. The ... The paper-based cryopreservation platform can be rolled or folded during storage to save space, and can be cut into small ... These findings, which are described in an article ("Paper‐Based Cell Cryopreservation") in the journal Advanced Biosystems, ... "The continuous development of simple and practical cell cryopreservation methods is of great importance to a variety of sectors ...
... Marlon A. Guerrero Department of Surgery, The University of Arizona, 1501 N. Campbell ... M. F. Herrera, C. S. Grant, J. A. Van Heerden, D. Jacobsen, A. Weaver, and L. A. Fitzpatrick, "The effect of cryopreservation ... C. R. McHenry, D. B. Stenger, and N. K. Calandro, "The effect of cryopreservation on parathyroid cell viability and function," ... M. F. Brennan, E. M. Brown, H. F. Sears, and G. D. Aurbach, "Human parathyroid cryopreservation: in vitro testing of function ...
... Service. The JAX Sperm Cryopreservation method was developed by our reproductive science experts and has ... Sperm Cryopreservation techniques were developed by JAX scientists and are an essential tool for efficient colony management. ...
Panis B, Swennen R (1995). "Cryopreservation of Germplasm of Banana and Plantain (Musa Species)". Cryopreservation of Plant ... Aside from the challenges involved with cryopreservation in general, an important hurdle, when developing cryopreservation ... While this cryopreservation conservation strategy can be used on all plants, it is often only used under certain circumstances ... Plant cryopreservation is a genetic resource conservation strategy that allows plant material, such as seeds, pollen, shoot ...
... while also reviewing the history of cryopreservation. Its most unique feature is the breadth of its coverage, from basic ... information on reproduction in fishes, to such advanced topics as embryo cryopreservation. ... This edited book addresses central issues in fish gamete cryopreservation and breeding, ... along with detailed information on embryo cryopreservation in fishes and crustaceans. The role of cryopreservation in ...
Cryopreservation is the branch of science in which the effects of very low temperature on living beings at cellular level are ... Massip A (2001) Cryopreservation of embryos of farm animals. Reprod Domes Anim 36(2):49-55CrossRefGoogle Scholar ... Rall WF, Fahy GM (1985) Ice-free cryopreservation of mouse embryos at −196°C by vitrification. Nature 313:573-575CrossRefPubMed ... Friedler S, Giudice LC, Lamb EJ (1988) Cryopreservation of embryos and ova. Fertil Steril 49(5):743-764CrossRefPubMedGoogle ...
P. C. Stanwood, "Cryopreservation of seed germplasm for genetic conservation," in Cryopreservation of Plant Cells and Organs, K ... Seed Cryopreservation of Some Medicinal Legumes,. Journal of Botany,. vol. 2012. ,. Article ID 186891. ,. 7. pages. ,. 2012. . ... Seed Cryopreservation of Some Medicinal Legumes. Alla B. Kholina1 and Nina M. Voronkova1. 1Institute of Biology and Soil ... P. Chmielarz, "Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra," Acta Biologica Hungarica, vol. 61, no. 2, ...
... by R. Michael Perry. [Update of the article Options for Brain-Threatening Disorders ... Resuscitation from cryopreservation is a subject that has many divergent points of view even among those who accept the basic ... Though cryonicists see cryopreservation as a medical procedure, legally it qualifies as "disposal of a dead body" (or other ... Better still would be to have cryopreservation treated as a medical procedure which could be freely chosen and started at any ...
... by Aschwin de Wolf The goal of human cryopreservation standby and ... The choice of diprivan is a typical example of the sort of trade off that sometimes needs to be made in human cryopreservation ... In human cryopreservation vasopressors (pressors) are used to increase blood pressure and selectively shift blood flow to the ... Because avoiding some of the side effects of these medications is not as high a priority in human cryopreservation as in ...
Download this Cryopreservation photo now. And search more of iStocks library of royalty-free stock images that features Animal ...
Natures antifreeze inspires revolutionary bacteria cryopreservation technique *Synthetic reproductions of antifreeze proteins ... The Warwick team, led by Professor Matthew I. Gibson, has developed a new method for cryopreservation, inspired by the process ... By combining two polymers to slow ice growth during cryopreservation, the researchers were able to recover more bacteria after ... Natures antifreeze inspires revolutionary bacteria cryopreservation technique * UKs first access course in Islamic Education ...
... which made it practically unsuitable for cryopreservation. With that in mind, the team studied the characteristics of the ... scientists from the Australian Institute of Marine Science and the Taronga Conservation Society in coral reef cryopreservation ...
Effects of cryopreservation on lymphocyte immunophenotype and function.. Costantini A1, Mancini S, Giuliodoro S, Butini L, ... Cryopreservation of isolated peripheral blood mononuclear cells (PBMCs) for phenotypic and functional analyses is considered a ... We thus conclude that cryopreservation induces a consistent set of changes in PBMCs from both healthy and HIV-infected ... However, only few and somewhat conflicting data are presently available on the effects of cryopreservation on PBMCs, especially ...
Researchers report the first evidence of cryopreservation by an overwintering insect in which stores of an uncommon lipid are ... Researchers show that tissues are more likely than single cells to suffer damage during cryopreservation because of the tight ...
JAX cryopreservation followed by high success cryorecovery rates makes your strains available for project reinitiation or ... Mouse Cryopreservation, Recovery and Strain Submission. JAX is the global leader in cryopreservation and recovery. Harness our ... Mouse Cryopreservation. Protect your valuable strains from unfortunate events. JAX Cryopreservation Services offer fast and ... or natural disasters with JAX Cryopreservation and Recovery Services. ...
NutriFreez® brand of cryopreservation media has set the standard for high-quality freezing solutions. It is the first fully ... NutriFreez® D10 Cryopreservation Medium without Phenol Red Item no.: 05-714-1B ... Powerful cryopreservation media optimized for enhanced performance across a broad range of cells. ... NutriFreez® Media maintains animal component-free conditions during cryopreservation, essential to maintaining consistency when ...
CONCLUSION: The data may help establishing better cryopreservation protocols for perspective chorionic cell lines and their ... OBJECTIVE: To investigate the effect of cryopreservation on proliferation and differentiation potential of chorion cell culture ... CryoLetters is a bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or ...
Ovarian tissue cryopreservation Survival of tissue undergoing cryopreservation, thawing and maturation of immature eggs. ... Cryopreservation of Ovarian Tissue. The safety and scientific validity of this study is the responsibility of the study sponsor ... Ovarian tissue cryopreservation. Ovarian tissue transplantation. Fertility preservation. Risk of premature ovarian failure. ... CRYOPRESERVATION OF OVARIAN TISSUE FOR POTENTIAL IN VITRO MATURATION OR AUTOLOGOUS TRANSPLANTATION [ Time Frame: 6 months to a ...
The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for ... Embryo Cryopreservation The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and ... The Process of Cryopreservation. At the Emory Reproductive Center, the mechanical process of embryo cryopreservation is ... Advantages of Cryopreservation. The Ethics Committee of the American Society of Reproductive Medicine has suggested the ...
Upon death, her body was cryopreserved--frozen--and relocated to a cryopreservation facility called the Alcor Life Extension ...
Current Frontiers in Cryopreservation. Edited by: Igor I. Katkov. ISBN 978-953-51-0302-8, PDF ISBN 978-953-51-4326-0, Published ... Current Frontiers in Cryopreservation. Edited by Igor Katkov. Institute for Medical Research, United States of America ... Marine Fish Sperm Cryopreservation and Quality Evaluation in Sperm Structure and Function. By Qing Hua Liu, Zhi Zhong Xiao, Shi ... Review on Ovarian Cryopreservation in Large Animals and Non-Human Primates. By Milan Milenkovic, Cesar Díaz-Garcia and Mats ...
Maximizing the retention of antigen specific lymphocyte function after cryopreservation.. Disis ML1, dela Rosa C, Goodell V, ... We determined several factors which do not affect cell viability after cryopreservation such as shipping frozen samples on dry ... We evaluated a variety of cryopreservation strategies with the aim of developing an optimized protocol for freezing and thawing ... we used an optimized cryopreservation protocol with different media additives to determine if functional T cell responses to ...
Only droplet-vitrification ensured survival and regrowth after cryopreservation. After cryopreservation, regeneration of apical ... Cryopreservation of Redwood (Sequoia Sempervirens (D. DON.) ENDL.) in Vitro Buds Using Vitrification-Based Techniques ... In this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation- ... With basal buds, regeneration after cryopreservation was possible over a larger range of PVS2 treatment durations, between 30 ...
Cryopreservation. Latest News. Latest developments in the freeze drying of red blood cells using biomimetic apatites. A ...
  • The JAX Sperm Cryopreservation method was developed by our reproductive science experts and has become the industry standard. (jax.org)
  • Insure your strain from loss with our Sperm Cryopreservation Service. (jax.org)
  • Sperm Cryopreservation techniques were developed by JAX scientists and are an essential tool for efficient colony management. (jax.org)
  • The project manager, Prof. Robert Patzner from Paris Lodron University of Salzburg (Department of Organismic Biology) explains the advantages of sperm cryopreservation: "Although it is, in principle, technically possible to store eggs instead of sperm, the size, shape and contents of eggs are problematic for cold storage. (scienceblog.com)
  • Sperm cryopreservation (commonly called sperm banking or sperm freezing) is a procedure to preserve sperm cells. (myhealth.gov.my)
  • Sperm Cryopreservation is available for men who wish to bank sperm for future use. (myhealth.gov.my)
  • In Hospital Kuala Lumpur, sperm cryopreservation is done for Oncology patient, Neurology patient, as a back up for Assisted Reproductive Treatment (ART) backup and for men with severe oligospermia (sperm count less than 5×106/ml) who wish to do ART. (myhealth.gov.my)
  • Patients are typically referred to the Andrology Laboratory for sperm cryopreservation by their clinician. (myhealth.gov.my)
  • Sperm cryopreservation allows future use of sperm in fertility treatments such as Intra Uterine Insemination (IUI) or In Vitro Fertilization (IVF) or Intracytoplasmic Sperm Injection (ICSI). (myhealth.gov.my)
  • Subject: antioxidants useless for sperm cryopreservation Am J Vet Res. (cryonet.org)
  • There are a number of reasons to consider sperm cryopreservation (freezing). (pacificfertility.ca)
  • ABSTRACT: In 2013, the American Society for Reproductive Medicine and the Society for Assisted Reproductive Technology published a joint document, Mature Oocyte Cryopreservation: A Guideline, which addresses advances in techniques to freeze human eggs that have resulted in significant recent improvements in pregnancy success. (acog.org)
  • There are not yet sufficient data to recommend oocyte cryopreservation for the sole purpose of circumventing reproductive aging in healthy women. (acog.org)
  • However, as stated in the ASRM-SART guideline, "there are not yet sufficient data to recommend oocyte cryopreservation for the sole purpose of circumventing reproductive aging in healthy women because there are no data to support the safety, efficacy, ethics, emotional risks, and cost-effectiveness of oocyte cryopreservation for this indication" (1). (acog.org)
  • Mature oocyte cryopreservation is a currently available method of fertility preservation in women of reproductive age. (acog.org)
  • An important clinical predictor of outcomes in the observational studies of oocyte cryopreservation and IVF is the age of the oocyte when frozen or vitrified ( 13-16 ). (acog.org)
  • Chen C (1986) Pregnancy after human oocyte cryopreservation. (springer.com)
  • Clark NA, Swain JE (2013) Oocyte cryopreservation: searching for novel improvement strategies. (springer.com)
  • The chapters in this book are divided into five parts and cover subjects such as: immature oocyte cryopreservation, human sperm vitrification and slow-freezing, directional freezing of ovarian tissue, automated vitrification systems, and detailed protocols on popular and commercially available cryopreservation/vitrification systems. (springer.com)
  • DALLAS, Texas, May 23, 2011 (SEND2PRESS NEWSWIRE) - Women delaying childbearing or requiring fertility preservation prior to undergoing treatment now have an option with the launch of oocyte cryopreservation at Fertility Specialists of Texas. (send2press.com)
  • Human egg freezing or oocyte cryopreservation is a novel technique intended to preserve a woman's eggs for later use in life. (send2press.com)
  • Oocyte cryopreservation is a technique with considerable potential in reproductive medicine, including fertility preservation, as a way of delaying childbearing and as part of oocyte donation programs. (who.int)
  • To compare the effectiveness and safety of vitrification and slow freezing as oocyte cryopreservation techniques for fertility outcomes in women undergoing assisted reproduction. (who.int)
  • Scientists report for the first time the ability to both deep freeze and reanimate zebrafish embryos using the process of cryopreservation, a method that could potentially be used to replenish the ocean's biodiversity. (britannica.com)
  • Although in vitro fertilization (IVF) with cryopreservation of embryos is an established method of fertility preservation, it requires that the patient have a male partner or be willing to use donor sperm. (acog.org)
  • Friedler S, Giudice LC, Lamb EJ (1988) Cryopreservation of embryos and ova. (springer.com)
  • JAX Cryopreservation Services offer fast and reliable recovery of archived embryos and sperm so you can feel assured about the safety of your strains. (jax.org)
  • The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for future use. (emoryhealthcare.org)
  • Only high quality embryos are selected for cryopreservation since poor quality embryos seldom survive the freezing and thawing processes. (emoryhealthcare.org)
  • Reduction of the risk of triplets or quadruplets by using cryopreservation to store embryos that exceed an optimal number for transfer. (emoryhealthcare.org)
  • Cutting-edge and practical, Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols includes chapters written by leading experts in the field and is a valuable resource for anyone interested in the field of cryopreservation. (springer.com)
  • The Sydney IVF Cryopreservation Kit is intended for use during in vitro fertilization procedures for cryopreservation of 1-cell to 8-cell embryos. (cookmedical.com)
  • Cryopreservation may be used to preserve embryos, eggs, sperm, and ovarian tissue, which may allow for childbirth when it would otherwise not be possible. (forensisgroup.com)
  • For decades, fertility clinics utilized a slow freeze technique for cryopreservation (freezing of eggs and embryos). (fertilityauthority.com)
  • In the past, the best (most successful) option was to cryopreserve embryos, but now egg cryopreservation provides similar outcomes. (fertilityauthority.com)
  • ATCC has the tools you need for the successful cryopreservation of your microbial cultures. (atcc.org)
  • 130°C) is entirely sufficient for successful cryopreservation. (sarstedt.com)
  • Our understanding of the role of MMP in freeze-thaw death and of the characteristics of the rGLUT2 transporter will lead to the development of more successful cryopreservation protocols. (mit.edu)
  • The key to successful cryopreservation is the ability to warm the embryo fast enough so no ice crystals form in it, Khosla said. (mndaily.com)
  • The first successful cryopreservation studies were performed in 1949 (1), and since then, numerous strategies have been proposed and adopted to facilitate the rapid restoration of cellular function following freezing. (pda.org)
  • Embryo cryopreservation is an efficient, reliable, and cost-effective means of safeguarding and maintaining stocks of critically GEM resources. (bcm.edu)
  • Moreover, energetics studies of gametes have become essential, considering the loss of vigour in the spermatozoa after cryopreservation.The latest development in this context is the cryopreservation of spermatogonial stem cell, which is also covered in the book, along with detailed information on embryo cryopreservation in fishes and crustaceans. (springer.com)
  • Its most unique feature is the breadth of its coverage, from basic information on reproduction in fishes, to such advanced topics as embryo cryopreservation. (springer.com)
  • Camus M (2004) Human embryo cryopreservation: review of clinical issues related to the success rate. (springer.com)
  • The quality of the embryo cryopreservation program at Emory is reflected in the pregnancy rates achieved with frozen embryo transfer. (emoryhealthcare.org)
  • At the Emory Reproductive Center, the mechanical process of embryo cryopreservation is laboratory controlled to reduce the risk of technical failure. (emoryhealthcare.org)
  • MMRRC at UNC offers embryo cryopreservation as a highly effective way to preserve your line. (unc.edu)
  • Embryo cryopreservation is recommended for lines with multiple genetic mutations or uncommon/mixed backgrounds. (unc.edu)
  • In vitro Fertilization (IVF) and embryo cryopreservation can be highly variable between strains as well as with individual mice. (unc.edu)
  • It is recommended that 2 males (10-24 weeks of age) and at least 10 females (3-12 weeks of age) be submitted for embryo cryopreservation. (unc.edu)
  • These can have a significant impact on the IVF and embryo cryopreservation success rates. (unc.edu)
  • How Has Vitrification Improved Egg and Embryo Cryopreservation? (fertilityauthority.com)
  • Home - Egg Freezing - Vitrification - How Has Vitrification Improved Egg and Embryo Cryopreservation? (fertilityauthority.com)
  • Fertility Specialists of Texas provides advanced fertility services to the greater North Texas area, including in vitro fertilization (IVF), donor sperm and donor eggs, embryo freezing or cryopreservation, ICSI, embryo donation and other advanced treatments for both male and female infertility. (send2press.com)
  • Effects of cryopreservation on lymphocyte immunophenotype and function. (nih.gov)
  • However, only few and somewhat conflicting data are presently available on the effects of cryopreservation on PBMCs, especially in samples from HIV-infected patients in which assessment of lymphocyte phenotype and function is of the outmost importance. (nih.gov)
  • Market leaders in temperature controlled microscopy, Linkam Scientific Instruments report on the use of their temperature controlled stages in the study of the effects of cryopreservation of Koala spermatozoa in Australia. (news-medical.net)
  • Retrieved on April 10, 2020 from https://www.news-medical.net/news/20150428/Linkam-stages-used-to-study-the-effects-of-cryopreservation-on-the-quality-of-Koala-spermatozoa-samples.aspx. (news-medical.net)
  • The aim of this study was to examine the effects of cryopreservation on the chondrogenic differentiation characteristics of human mesenchymal derived stem cells isolated from adipose tissue and encapsulated in κ-carrageenan hydrogels. (rsc.org)
  • In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use. (atcc.org)
  • To complete the serum-free product range, MP introduces CellVationtm, a unique cryopreservation medium which does not contain either DMSO or serum. (mpbio.com)
  • What should we use as medium for cryopreservation of human esophageal cancer cell line - FBS with 10% DMSO OR its own medium with 10% DMSO? (biology-online.org)
  • BG01V human embryonic stem cells were frozen in CryoDefend ® Stem Cells Media (Catalog # CCM018), conventional freezing media (90% FBS/10% DMSO) or cryopreservation media from a competitor. (rndsystems.com)
  • Several protocols have been developed for efficient cryopreservation of cells and tissues, and a combination of dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS) is commonly used. (sigmaaldrich.com)
  • i am very new to cell culture and neglected to add a cryopreservation agent (DMSO or glycerol) to cells before freezing them. (protocol-online.org)
  • Engineered cryopreservation media such as the CryoStor brand and home-brew cocktails such as culture media with the cryoprotectant dimethyl sulfoxide (DMSO) can work adequately for a number of cell types. (bioprocessintl.com)
  • Dehydration improves cryopreservation of coconut (Cocos nucifera L.)". Cryobiology. (wikipedia.org)
  • Recently, there have been some serious tectonic shifts in cryobiology which were perhaps not seen on the surface but will have a profound effect on both the future of cryobiology and the development of new cryopreservation methods. (intechopen.com)
  • The present books, "Current Frontiers in Cryobiology" and "Current Frontiers in Cryopreservation" will serve the purpose. (intechopen.com)
  • Advancements in cryobiology, the study of biological systems at a very low temperature, have led many to believe that immortality may be achievable through cryopreservation. (jdsupra.com)
  • Having considered both the national trend towards increased constitutional privacy protection and the substantial advancements in cryobiology and other supporting sciences, this note argues that courts should recognize a terminally-ill brain cancer patient's constitutional right to receive assistance in achieving pre-mortem cryopreservation. (jdsupra.com)
  • Cryopreservation , the preservation of cells and tissue by freezing . (britannica.com)
  • Today there is intense interest in the growth of these cells in tissue culture systems, as well as in the cryopreservation of these cells for future therapy for a wide variety of disorders, including disorders of the nervous and muscle systems and diseases of the liver and heart . (britannica.com)
  • These findings, which are described in an article (" Paper‐Based Cell Cryopreservation ") in the journal Advanced Biosystems , provide a promising prospect for industrial cell banks and researchers engaged in tissue engineering, drug testing, and other fields that rely on long-term, successful preservation of cells, said Mohammad A. Qasaimeh, PhD, assistant professor of mechanical and biomedical engineering. (genengnews.com)
  • Even within the plant itself there can be noticeable differences depending on the tissue that is used, as both structure and composition play an important role during cryopreservation. (wikipedia.org)
  • The Human Tissue Authority (HTA) has produced this information to provide an overview of cryonics - cryopreservation of whole bodies. (hta.gov.uk)
  • Cryogenics does not necessarily have anything to do with biology or preserving tissue, although sometimes the term is used interchangeably with cryopreservation, such as in medicine. (hta.gov.uk)
  • This volume provides a comprehensive and technical presentation of numerous aspects of reproductive cell tissue cryopreservation, and presents readers with current procedures and detailed discussions of novel techniques and the latest innovations. (springer.com)
  • To provide a novel fertility preservation option for patients facing a fertility threatening cancer diagnosis or treatment regimen by establishing an ovarian tissue cryopreservation program. (clinicaltrials.gov)
  • The hypothesis is that ovarian tissue cryopreservation for fertility preservation provides an alternative option for fertility preservation. (clinicaltrials.gov)
  • In addition to biological tissue found in humans, cryopreservation may also be used for studying fungi, moss, and various forms of bacteria. (forensisgroup.com)
  • The extracted tissue is then treated in antibiotic solution before being cooled and stored in the vapour phase of liquid nitrogen, a procedure known as cryopreservation. (sciencephoto.com)
  • Ovarian tissue cryopreservation (freezing) is an experimental method of fertility preservation in which the outer layer of an ovary, which contains a large number of immature eggs, is taken out of the body and frozen for future use. (centerforhumanreprod.com)
  • Combination with cryopreservation of ovarian tissue should therefore be considered. (endocrine-abstracts.org)
  • Cryopreservation based on freeze-drying does not require use of liquid-nitrogen freezers. (britannica.com)
  • Plant cryopreservation is a genetic resource conservation strategy that allows plant material, such as seeds, pollen, shoot tips or dormant buds to be stored indefinitely in liquid nitrogen. (wikipedia.org)
  • Cryopreservation (storage in liquid nitrogen (LN) at −196°C) is the only currently available long-term storage technique that ensures safe conservation of genetic resources of valuable plant species [ 14 - 16 ]. (hindawi.com)
  • The cryopreservation of spermatozoids allows a man's fertility to be preserved during oncological or medical treatment or MAR. After microscopic examination of the concentration and mobility of spermatozoids in the ejaculate, the latter is frozen and then stored in liquid nitrogen at -196°C. (chuv.ch)
  • BG01V human embryonic stem cells were frozen in cryopreservation media from two different competitors or CryoDefend ® Stem Cells Media (Catalog # CCM018) at 1 x 10 6 cells/cryovial and stored in liquid nitrogen for one week. (rndsystems.com)
  • With any cryopreservation method, whole or partial parts of specimens which are sufficiently dehydrated can be vitrified by rapid cooling in liquid nitrogen (LN). Techniques discussed are the vitrification protocol, encapsulation-vitrification protocol, droplet vitrification protocol (DV), vitrification protocol using cryo-plates (V cryo-plate), and air dehydration protocol using cryo-plates (D cryo-plate). (springer.com)
  • columbinus and 1 from P. pulmonarius ) after liquid nitrogen cryopreservation (-196º) was evaluated. (scielo.br)
  • That is what prompted the present study, in which recovery after liquid nitrogen cryopreservation was examined in six strains of edible mushrooms of the Pleurotus genus. (scielo.br)
  • Researchers show that tissues are more likely than single cells to suffer damage during cryopreservation because of the tight junctions between cells. (the-scientist.com)
  • Cryopreservation is the process of preserving cells or complete tissues that are susceptible to damage caused by chemical reactivity and harmful enzymatic activity. (openpr.com)
  • Cryopreservation expert consulting may provide essential information for cases involving such areas as stem cell research, in vitro fertilization (IVF) and other reproductive issues, plants, and other types of organic tissues. (forensisgroup.com)
  • While preservation by freezing seems simple, freezing and thawing of cells and tissues typically compromise their integrity such that specialized protocols are needed for cryopreservation to be even partly successful. (europa.eu)
  • The unique interactions of AFPs with ice crystals suggest their potential as additives for cryopreservation of foods, cells, tissues, and organs. (europa.eu)
  • Cryo-scanning electron microscopy makes it possible to examine the cellular and water behavior in plant tissues when immersed in LN. It has been verified that tissues cryopreserved by the process of vitrification and the cryo-plate protocols are cryopreservation methods for reliable long-term preservation of PGRs. (springer.com)
  • The critical point of cryopreservation is the degree of moisture in the seeds that have gone through this process, because if there is little water in their tissues, they will suffer from excessive dehydration and will not survive. (scielo.br)
  • In this study, we investigated the effects of human plasma as a cryopreservation solution for human adipose tissues. (omicsonline.org)
  • We did not observe any adherent cells when human adipose tissues were preserved without cryopreservation solution at ?20°C. Adherent adipose-derived cells exhibited different growth rates based on the cryopreservation solution and storage temperature. (omicsonline.org)
  • In conclusion, these results demonstrated that human plasma is useful as a cryopreservation solution for human adipose tissues. (omicsonline.org)
  • Morphology of Human Embryonic Stem Cells Following Cryopreservation in CryoDefend ® Stem Cells Media, Conventional Freezing Media, or Freezing Media from a Competitor. (rndsystems.com)
  • Expression of Pluripotency Markers in BG01V Human Embryonic Stem Cells Frozen in CryoDefend ® Stem Cells Media or Cryopreservation Media from Two Different Competitors. (rndsystems.com)
  • Human Embryonic Stem Cells Maintain Pluripotency Following Cryopreservation in CryoDefend ® Stem Cells Media. (rndsystems.com)
  • However, the viability of human iPS cells, like embryonic stem cells, decreases significantly during cryopreservation. (medindia.net)
  • Though cryonicists see cryopreservation as a medical procedure, legally it qualifies as "disposal of a dead body" (or other remains). (alcor.org)
  • A cryonicist wishing immediate cryopreservation thus must induce a state of cardiac and respiratory arrest or clinical death-suicide in the eyes of the law-before the procedure can begin. (alcor.org)
  • Cryopreservation of isolated peripheral blood mononuclear cells (PBMCs) for phenotypic and functional analyses is considered a standard procedure in order to minimize operator-dependent inter-assay variability and to optimize the use of available resources. (nih.gov)
  • Cryonics is an experimental cryopreservation procedure using cryoprotectants and temperatures so cold that a person beyond help by today's medicine might be preserved for decades or centuries until a future medical technology can restore that person to full health. (kurzweilai.net)
  • In this article, we will discuss definition, procedure and risks of cryopreservation. (worldwidehealth.com)
  • With this procedure, more than 23 adventitious buds were produced in each leaf explant, and 100% of SLSs were able to survive and resume shoot regrowth, with more than six shoots per SLS obtained following cryopreservation in three highbush blueberry cultivars. (springer.com)
  • In the ideal case, the cryopreservation procedure begins immediately after the person is pronounced clinically dead, ensuring the brain is cooled and preserved before any damage can occur. (jdsupra.com)
  • The Ethics Committee of the American Society of Reproductive Medicine has suggested the following advantages of cryopreservation. (emoryhealthcare.org)
  • While cryopreservation is used for numerous medical purposes, among other scientific areas of research, it is most commonly utilized for reproductive issues. (forensisgroup.com)
  • In their latest workplace revolution, these companies are now covering cryopreservation for women, a service conducted at Pennsylvania Reproductive Specialists . (sbwire.com)
  • For those interested in cryopreservation and would like to consult a fertility doctor near Philadelphia , visit Pennsylvania Reproductive Specialists online or call 215-822-8400 today. (sbwire.com)
  • Cryopreservation of human spermatozoa: comparison of two cryopreservation methods and three cryoprotectants. (myhealth.gov.my)
  • Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants. (cryonet.org)
  • OBJECTIVE: To evaluate the effect of the addition of enzyme scavengers and antioxidants to the cryopreservation extender on characteristics of equine spermatozoa after freezing and thawing. (cryonet.org)
  • CONCLUSIONS AND CLINICAL RELEVANCE: The addition of antioxidants to the cryopreservation extender did not improve the quality of equine spermatozoa after thawing, which suggests that the role of oxidative stress in cryopreservation-induced damage of equine spermatozoa requires further investigation. (cryonet.org)
  • Head morphomertic characteristics of spermatozoa during the cryopreservation process for the three patterns of response. (plos.org)
  • One that has been highlighted is how incorrect cryopreservation can lead to adverse effects on the motility of spermatozoa samples. (news-medical.net)
  • The study found that the cryopreservation of spermatozoa samples significantly and irreversibly reduced the proportion of cells with a high level of MMP which of course has negative knock on effects for fertility. (news-medical.net)
  • The study also looked at how cryopreservation affects the plasma membrane of the cell, and whether or not cryoprotectants such as Glycerol may improve the viability of spermatozoa. (news-medical.net)
  • Cryopreservation induces differential remodeling of the proteome in mammalian spermatozoa. (diva-portal.org)
  • Cryopreservation quantitatively altered more proteins in ejaculated than cauda epididymal spermatozoa. (diva-portal.org)
  • Differential protein-protein networks highlighted a set of proteins quantitatively altered in ejaculated spermatozoa, directly involved in mitochondrial functionality which would explain why ejaculated spermatozoa deteriorate during cryopreservation. (diva-portal.org)
  • Two Finnish students have finished their bachelor theses in which they assessed the selection of Finnhorse stallions for semen cryopreservation. (nordgen.org)
  • Saija Tenhunen and Tytti Salonpää evaluated cryopreservation options of stallion semen in their bachelor theses. (nordgen.org)
  • Prof. Patzner and Dr. Mansour are keenly aware that semen cryopreservation cannot compensate for the worldwide destruction of the natural habitat of amphibians. (scienceblog.com)
  • Semen collection and AI makes semen cryopreservation and germ plasma preservation possible. (usgs.gov)
  • Yet, semen cryopreservation techniques need improvement before fertility with frozen-thawed semen will equal fertility from AI with fresh semen. (usgs.gov)
  • Semen cryopreservation might be influenced by sperm concentration. (magiran.com)
  • Following the data of the German, Swiss and Austrian network Fertiprotekt ( www.fertiprotekt.eu ), 164 of 1388 counselled patients have chosen ovarian stimulation and cryopreservation of oocytes as a fertility preservation technique in 2007 2009. (endocrine-abstracts.org)
  • What is ovarian cortex cryopreservation? (cancercenter.com)
  • This process is performed in an apparatus known as cryopreservation equipment. (openpr.com)
  • To investigate the effect of cryopreservation on proliferation and differentiation potential of chorion cell culture (ChCC). (ingentaconnect.com)
  • We evaluated a variety of cryopreservation strategies with the aim of developing an optimized protocol for freezing and thawing PBMC to retain viability and function. (nih.gov)
  • A wide variety of cryopreservation solutions have been used, however many are toxic or ineffective for use in extended cryopreservation. (medindia.net)
  • Fahy GM, MacFarlane DR, Angell CA, Meryman HT (1984) Vitrification as an approach to cryopreservation. (springer.com)
  • Fabre J, Dereuddre J (1990) Encapsulation-dehydration: a new approach to cryopreservation of Solanum shoot tips. (springer.com)
  • Cryopreservation is the branch of science in which the effects of very low temperature on living beings at cellular level are observed in order to standardize efficient protocols for cryopreservation. (springer.com)
  • We feel that it is time to revise the previous paradigms and dogmas, discuss the conceptually new cryobiological ideas, and introduce the recently emerged practical protocols for cryopreservation. (intechopen.com)
  • Resuscitation from cryopreservation is a subject that has many divergent points of view even among those who accept the basic idea of cryonics. (alcor.org)
  • This information covers cryonics, the cryopreservation of whole bodies. (hta.gov.uk)
  • Information on cryonics, the cryopreservation of whole bodies and cryogenics. (hta.gov.uk)
  • a specific type of cryopreservation, cryonics is the attempt to preserve a person's entire body after death. (hta.gov.uk)
  • The best scenario for cryonics would be improved, and possibly demonstrably reversible, cryopreservation of animal brains. (fightaging.org)
  • Because of the medicolegal constraints imposed upon cryonics now, and most likely in the foreseeable future, cryopreservation procedures cannot begin until clinical and legal death have occurred. (alcor.org)
  • In addition to funding from individuals, the Life Extension Foundation (LEF) in Fort Lauderdale, Florida is donating $10,000 toward Winborn's cryopreservation costs and LongeCity has raised $2,000 through a matching campaign drive by the William O'Rights Memorial Cryonics Charity Fund. (kurzweilai.net)
  • The results obtained show that the hydrogels withstand the cryopreservation with dimethyl sulfoxide, maintaining their structural integrity, while assisting cells proliferation and chondrogenic potential after cryopreservation. (rsc.org)
  • Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. (atcc.org)
  • Unlike other scaffold-based cryopreservation strategies such as fiber meshes and nanofiber sheets, where the substrates must be repeatedly engineered for their use in cell cryopreservation, this paper-based method is solely based on the ready-to-use papers where cells are preserved with no significant effect on their viability and metabolic activity," she said. (genengnews.com)
  • The goal of human cryopreservation standby and stabilization procedures is to preserve the structure and viability of the brain after medico-legal pronouncement of death. (alcor.org)
  • We determined several factors which do not affect cell viability after cryopreservation such as shipping frozen samples on dry ice, the length of time and speed at which samples are washed and centrifuged after thawing, and the number of cells frozen per container. (nih.gov)
  • Immediately after thawing, the viability percentages in all groups were significantly lower than before cryopreservation (p = 0.0369). (biomedsearch.com)
  • Finally, the Court explained that this case differs because the art taught that cryopreservation steps could only be performed once the cellular viability was compromised, and the claimed process was directed to repetition of cryopreservation steps. (lexology.com)
  • RESULTS: The addition of enzyme scavengers or antioxidants to cryopreservation extender did not improve spermatozoal motility, DNA fragmentation, acrosomal integrity, viability, or mitochondrial membrane potential after thawing. (cryonet.org)
  • Human Embryonic Stem Cell Viability Following Cryopreservation in CryoDefend ® Stem Cells Media or Cryopreservation Media from Two Competitors. (rndsystems.com)
  • In the past 10 years, methods for ultrarapid freezing (vitrification) of oocytes have been refined that optimize oocyte survival after cryopreservation (1, 5-7 ). (acog.org)
  • Cryopreservation of oocytes will have multiple applications for animals and human beings. (springer.com)
  • Vitrification is a cryopreservation method that can be used to preserve human oocytes. (who.int)
  • Nobody is too young to make cryopreservation arrangements. (kbia.org)
  • Keep in mind that process development folks tend to be engineers or manufacturing personnel, not 'basic scientists' who want to make cryopreservation a research project. (bioprocessintl.com)
  • The data may help establishing better cryopreservation protocols for perspective chorionic cell lines and their further application in biotechnology. (ingentaconnect.com)
  • Toward Better Cryopreservation: Xeno-Free, Reduced Toxicity Prot. (thermofisher.com)
  • This cryopreservation method can be considered an efficient cryopreservation of plant shoot tips, and has potential applications to other plant species. (springer.com)
  • Burritt DJ (2008) Efficient cryopreservation of adventitious shoots of Begonia × erythrophylla using encapsulation-dehydration requires pretreatment with both ABA and proline. (springer.com)
  • A team of researchers from Japan compared 12 kinds of commercially prepared and readily available cryopreservation solutions and found that "Cell Banker 3" out-performed the other 11 solutions by allowing iPS cells to be preserved for a year at degrees C in an undifferentiated state, in a study to determine the best cryopreservation (freezing) solution to maintain induced pluripotent stem (iPS) cells. (medindia.net)
  • For information on cryopreservation relating to fertility treatment, please refer to the Human Fertilisation and Embryology Authority (HFEA). (hta.gov.uk)
  • Cryopreservation is based on the ability of certain small molecules to enter cells and prevent dehydration and formation of intracellular ice crystals, which can cause cell death and destruction of cell organelles during the freezing process. (britannica.com)
  • The continuous development of simple and practical cell cryopreservation methods is of great importance to a variety of sectors, especially when considering the efficient short‐ and long‐term storage of cells and their transportation. (genengnews.com)
  • Here, a paper‐based cell cryopreservation method as an alternative to conventional cryopreservation methods is presented. (genengnews.com)
  • The paper platform, working as a 3D shield and carrier for cells during cryopreservation, greatly simplifies the storage, management, and logistics of cell banking," continued Qasaimeh. (genengnews.com)
  • It is the first fully defined, optimized freezing solution designed and validated for the cryopreservation of various cell types, including hESCs, iPSCs, and hMSCs. (sartorius.com)
  • Finally, we used an optimized cryopreservation protocol with different media additives to determine if functional T cell responses to tetanus toxoid could be preserved. (nih.gov)
  • Cryopreservation equipment include drystore freezers, mechanical freezers, cryopreservation freezers, cryopreservation vials, incubators and stem cell research laboratory equipment. (openpr.com)
  • Slow cooling cryopreservation of cell-microcarrier constructs. (biomedsearch.com)
  • Cellvation is a unique cryopreservation medium designed for optimal cell recovery and convenience. (mpbio.com)
  • Cryopreservation is also used for preserving stem cells, which may be utilized in stem cell therapy for the prevention and treatment of different diseases and other medical conditions. (forensisgroup.com)
  • Cryopreservation is essential for the banking of valuable stem cell cultures. (rndsystems.com)
  • R&D Systems offers CryoDefend ® Stem Cells Media, a defined, protein-free media, which has been optimized for stem cell cryopreservation. (rndsystems.com)
  • Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media. (sigmaaldrich.com)
  • Additional cell lines/clones can be expanded and grown in sufficient quantities to produce 4 vials for cryopreservation. (rockland-inc.com)
  • Unfortunately, despite decades of research and increasing emphasis in the clinic, harmonized cryopreservation standards for cell and gene therapies (Advanced Therapy Medicinal Products or ATMPs) do not exist, and cryopreservation protocols vary widely in practice both between, and even within, commercial and academic groups. (pda.org)
  • Cryopreservation Cell Lines Market Outlook 2020 Sales Rev. (pharmiweb.com)
  • Future Market Insights (FMI) presents its new, comprehensive study on the global Cryopreservation cell lines market spanning from 2019 to 2029. (pharmiweb.com)
  • This is sure to impact the growth of the Cryopreservation cell lines market through the pandemic period. (pharmiweb.com)
  • FMI's report includes a dedicated section expounding both the short-term and long-term impact of the pandemic on the Cryopreservation cell lines market. (pharmiweb.com)
  • The study bestows valuable insights into the competitive landscape of the global Cryopreservation cell lines market, by studying numerous players, their growth strategies, and key developments. (pharmiweb.com)
  • What will be the key driving factor propelling the demand for Cryopreservation cell lines during the forecast period? (pharmiweb.com)
  • How current socio-economic trends will impact the Cryopreservation cell lines market? (pharmiweb.com)
  • What are the growth strategies implemented by prominent players in the Cryopreservation cell lines market to maintain their foothold in the competitive landscape? (pharmiweb.com)
  • His opinion is that developers of cell therapy and regenerative medicine products often focus first on cell characterization, method of action, and potency assays, and allow cryopreservation (or any biopreservation processing steps) to become the weak link in their bioprocessing work f low chain. (bioprocessintl.com)
  • If you put a lot of time and effort into your cell therapy and then just freeze it down into a freeze media that you either make in-house or buy from a supplier, are you actually spending the same amount of time and energy making sure that your cryopreservation process is actually just as strong as the rest of your system? (bioprocessintl.com)
  • Cell lines are stored by cryopreservation. (dsmz.de)
  • Cryopreservation methods seek to reach low temperatures without causing additional damage caused by the formation of ice crystals during freezing. (wikipedia.org)
  • A sugar called trehalose , which occurs in organisms capable of surviving extreme dehydration, is used for freeze-drying methods of cryopreservation. (britannica.com)
  • Scientists from the division of engineering at NYU Abu Dhabi (NYUAD) report that they have developed a new technique that utilizes filter paper to cryopreserve human cells, offering scientists an alternative to conventional, long-term cryopreservation methods. (genengnews.com)
  • By combining two polymers to slow ice growth during cryopreservation, the researchers were able to recover more bacteria after freezing than using conventional methods. (warwick.ac.uk)
  • However, despite the clear advantages that sperm offers for cryopreservation, there is still a great deal of potential for optimising methods, and this is precisely the area that Prof. Patzner and his colleague Dr. Nabil Mansour are investigating. (scienceblog.com)
  • New and improved cryopreservation methods are therefore required. (europa.eu)
  • We developed methods for the cryopreservation of sperm of the endangered Colorado pikeminnow Ptychocheilus lucius. (usgs.gov)
  • Richard Paulson, M.D., Director of USC Fertility in Southern California explains that vitrification has significantly improved methods of cryopreservation and continues to improve as research findings emerge. (fertilityauthority.com)
  • Although there are several biopreservation techniques (indicated in "Biopreservation Methods" box), most laboratories use either standard cryopreservation protocols (the far majority) or vitrification (much more limited in broad systems application) when freezing cells for research and clinical applications. (bioprocessintl.com)
  • An important application of cryopreservation is in the freezing and storage of hematopoietic stem cells , which are found in the bone marrow and peripheral blood. (britannica.com)
  • The paper-based cryopreservation platform can be rolled or folded during storage to save space, and can be cut into small pieces during the retrieval of cells. (genengnews.com)
  • Powerful cryopreservation media optimized for enhanced performance across a broad range of cells. (sartorius.com)
  • NutriFreez® Media maintains animal component-free conditions during cryopreservation, essential to maintaining consistency when culturing cells in a xeno-free system. (sartorius.com)
  • Cryopreservation of human heart cells. (biomedsearch.com)
  • The report also calculates the forthcoming status of Cryopreservation Equipment in Stem Cells market based on thorough analysis. (openpr.com)
  • Top Companies involved in the Global Cryopreservation Equipment in Stem Cells Market are Thermo Fisher Scientific, Charter Medicals, Linde Gas Cryoservices, praxair and other. (openpr.com)
  • Comprehensive assessment of all opportunities and risk in Cryopreservation Equipment in Stem Cells market. (openpr.com)
  • Cryopreservation Equipment in Stem Cells market recent innovations and major events. (openpr.com)
  • Detailed study of business strategies for growth of Cryopreservation Equipment in Stem Cells market-leading players. (openpr.com)
  • Conclusive study about the growth plot of Cryopreservation Equipment in Stem Cells market for forthcoming years. (openpr.com)
  • Finally, all aspects of the Global Cryopreservation Equipment in Stem Cells Market are quantitatively as well qualitatively assessed to study the Global as well as regional market comparatively. (openpr.com)
  • Whole blood cryopreservation is a cost-effective approach to large-scale storage of viable cells in epidemiological studies. (aacrjournals.org)
  • On July 5, 2016, the Federal Circuit vacated and remanded a district court decision holding that U.S. Patent No. 7,604,929 , which is directed to a method of cryopreservation of hepatocytes (liver cells) was invalid under 35 U.S.C. § 101 because it was directed to a patent-ineligible concept. (lexology.com)
  • The prior art generally taught that, once cryopreservation was performed once on hepatocytes, the cells would not remain viable for further testing if frozen a second time. (lexology.com)
  • Before cryopreservation, cells should be characterized and checked for contamination. (thermofisher.com)
  • Morphological differences such as extent of membrane blebbing were observed as well, verifying that cells with a high MMP are more likely to survive the cryopreservation process. (mit.edu)
  • Media for defined, protein-free cryopreservation of stem cells. (rndsystems.com)
  • These additional considerations emphasize the importance of using cryopreservation media that has been specifically optimized for the cryopreservation of stem cells. (rndsystems.com)
  • Cells that were cryopreserved in CryoDefend ® Stem Cells Media (Catalog # CCM018) or cryopreservation media from one of two competitors CryoDefend ® Stem Cells Media (Catalog # CCM018) were thawed and cultured for four days in Mouse Embryonic Fibroblast Conditioned Media (Catalog # AR005 ). (rndsystems.com)
  • The cells were subjected to cooling and heating cycles using the Linkam BCS196 cryo-stage to replicate the exact freeze/thaw processes seen in AI cryopreservation techniques. (news-medical.net)
  • We isolated DNA, RNA, proteins, and so forth from cells following cryopreservation and hypothermic preservation (2-8 °C), and observed that apoptotic signaling/death pathways were initiated in addition to necrotic pathways. (bioprocessintl.com)
  • Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termed cryoprotectants . (wikipedia.org)
  • Conventional cryopreservation has depended on covering the material to be solidified with a class of particles named cryoprotectants. (omicsonline.org)
  • Cryopreservation is the use of low temperatures to preserve biological samples. (europa.eu)
  • Chen XL, Li JH, Xin X, Zhang ZE, Xin PP, Lu XX (2011) Cryopreservation of in vitro -grown apical meristems of Lilium by droplet-vitrification. (springer.com)
  • In this study, the efficiency of three vitrification-based cryopreservation techniques, i.e. vitrification, encapsulation-vitrification and droplet-vitrification were compared for cryopreserving Sequoia sempervirens apical and basal buds sampled from in vitro shoot cultures. (ingentaconnect.com)
  • Hirai D, Sakai A (2001) Recovery growth of plants cryopreservation by encapsulation-vitrification. (springer.com)
  • While this cryopreservation conservation strategy can be used on all plants, it is often only used under certain circumstances: 1) crops with recalcitrant seeds e.g. avocado, coconut 2) seedless crops such as cultivated banana and plantains or 3) crops that are clonally propagated such as cassava, sweet potato. (wikipedia.org)
  • The role of cryopreservation in conservation programmes is another important aspect, one that will especially interest biologists.This book addresses central issues in fish gamete cryopreservation and breeding, while also reviewing the history of cryopreservation. (springer.com)
  • This said, conservation of the genetic diversity in the long term should be of top priority to guarantee vitality of the Finnhorse population also in the future, thus ex-situ cryopreservation is the recommended. (nordgen.org)
  • This event will feature the first details about a revolutionary research project to achieve perfected human cryopreservation. (fightaging.org)
  • The Warwick team, led by Professor Matthew I. Gibson, has developed a new method for cryopreservation, inspired by the process by which organisms known as extremophiles, survive in some of the coldest regions on Earth. (warwick.ac.uk)
  • These equipment parts help in completing the process of cryopreservation by providing stable cryogenic storage for biological specimens. (openpr.com)
  • Cryopreservation refers to the process of freezing different types of biological material at extreme temperatures as a means of preserving the material in question. (forensisgroup.com)
  • Similarly, the cryopreservation process varied in length from immediate freezing (LN 2 immersion) to slow cooling over 8 hours. (pda.org)
  • And, while process pause steps were not typically validated as part of the workflow (65%), a large majority (73%) incorporated stability studies to ensure the cryopreservation process did not impact sample integrity. (pda.org)
  • This process is called cryopreservation or freezing. (reproductivefacts.org)
  • Cryopreservation was applied to human materials beginning in 1954 with three pregnancies resulting from the insemination of previously frozen sperm. (wikipedia.org)
  • The choice of diprivan is a typical example of the sort of trade off that sometimes needs to be made in human cryopreservation. (alcor.org)
  • The formation of blood clots during human cryopreservation cases is problematic for a variety of reasons. (alcor.org)
  • In human cryopreservation vasopressors (pressors) are used to increase blood pressure and selectively shift blood flow to the vital organs (including the brain). (alcor.org)
  • Because avoiding some of the side effects of these medications is not as high a priority in human cryopreservation as in conventional medicine, protocol and dosages may differ somewhat from current practice in cardiopulmonary resuscitation. (alcor.org)
  • It is hard to overestimate the importance of restoring adequate cerebral blood flow in the human cryopreservation patient. (alcor.org)
  • The Advances in Human Cryopreservation conference, sponsored by Suspended Animation Inc , starts on May 18th. (fightaging.org)
  • It is generally argued by proponents of human cryopreservation that significant intervals of ischemic injury need not be catastrophic, nor result in the irreversible compromise of mentation and identity. (alcor.org)
  • Subject: Premedication of the Human Cryopreservation Patient II UY-ernative So, what can be done? (cryonet.org)
  • Benson EE, Lynch PT, Stacey GN (1998) Advances in plant cryopreservation technology: current applications in crop plant biotechnology. (springer.com)
  • Aside from the challenges involved with cryopreservation in general, an important hurdle, when developing cryopreservation protocols for storage of plant germplasm, is that plants within a species can have a different tolerance to cryopreservation. (wikipedia.org)
  • Cryopreservation of Plant Germplasm I. Biotechnology in Agriculture and Forestry. (wikipedia.org)
  • Bajaj YPS (ed) (1995) Cryopreservation of plant germplasm I. Biotechnology in agriculture and forestry, vol 32. (springer.com)
  • Protect your strains from pathogen outbreaks, genetic drift, breeding cessation, or natural disasters with JAX Cryopreservation and Recovery Services. (jax.org)
  • The Optimal Contribution Selection (OCS) approach was used to calculate the individual genetic contributions to the current population, as well as assess which stallions would be the best candidates for cryopreservation. (nordgen.org)
  • A project run by the Austrian Science Fund FWF is currently attempting to optimise the cold storage - or cryopreservation - of sperm, which is a useful medium for the practical storage of genetic material. (scienceblog.com)
  • This chapter reviews the current techniques for cryopreservation of plant genetic resources (PGRs). (springer.com)
  • Cryopreservation at least preserves the genetic diversity of the males, and the main advantage is that we can infuse wild genetics into the captive fish with great ease," said Wilson. (outdoornews.com)
  • Marchant R, Power JB, Davey MR, Chartier-Hollis JM, Lynch PT (1993) Cryopreservation of pollen from two rose cultivars. (springer.com)
  • Luza JG, Polito VS (1988) Cryopreservation of English Walnut (Juglans regia L.) pollen. (springer.com)
  • We thus conclude that cryopreservation induces a consistent set of changes in PBMCs from both healthy and HIV-infected individuals, and that certain immunological studies of HIV-infected patients (i.e. studies of immune reconstitution following antiretroviral therapy in HIV-infected patients or studies of HIV-infectivity in vitro using CCR5-tropic strains) should be performed on fresh samples. (nih.gov)
  • Only droplet-vitrification ensured survival and regrowth after cryopreservation. (ingentaconnect.com)
  • This is where the cryopreservation and androgenesis techniques developed in this FWF project can provide an effective solution to help protect the survival of endangered species. (scienceblog.com)
  • 2001). The survival rate of sperm after cryopreservation is highly variable depends on the parameters before being frozen. (myhealth.gov.my)
  • The history of plant cryopreservation started in 1965 when Hirai was studying the biology activities that happened when biological samples were frozen. (wikipedia.org)
  • Upon death, her body was cryopreserved--frozen--and relocated to a cryopreservation facility called the Alcor Life Extension Foundation in Arizona. (kbia.org)
  • Frozen sperm can be used almost indefinitely after cryopreservation. (myhealth.gov.my)
  • The claims at issue are generally directed to a method of cryopreservation of hepatocytes that would enable the hepatocytes to withstand multiple rounds of freezing and thawing. (lexology.com)
  • George Seidel, a professor at Colorado State University who specializes in cryopreservation, called this method innovative. (mndaily.com)
  • We report a new cryopreservation method for highbush blueberry ( Vaccinium corymbosum ) using small leaf squares-bearing adventitious buds. (springer.com)
  • However, the vitrification method was less effective for cryopreservation of BY-2 than a simplified slow prefreezing method. (nii.ac.jp)
  • To summarize, our study advanced the basic mechanisms underlying AFP activity, expand their possible use, and seek to develop approaches for using such proteins in cryopreservation applications. (europa.eu)
  • In: Reed B (ed) Plant cryopreservation: a practical guide. (springer.com)
  • Cryopreservation is a technique that can be used to store plant material without a time limit, since it reduces metabolism to levels so low that all biochemical processes are practically paralyzed ( KARTHA, 1985 ). (scielo.br)
  • The techniques used in the cryopreservation of plants are controlling the rate of refrigeration, vitrification, encapsulation, dehydration, and dormant buds' preservation ( REED, 2008 ). (scielo.br)
  • Maximizing the retention of antigen specific lymphocyte function after cryopreservation. (nih.gov)