Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Cryoprotective Agents: Substances that provide protection against the harmful effects of freezing temperatures.Semen Preservation: The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Propylene Glycol: A clear, colorless, viscous organic solvent and diluent used in pharmaceutical preparations.Vitrification: The transformation of a liquid to a glassy solid i.e., without the formation of crystals during the cooling process.Freezing: Liquids transforming into solids by the removal of heat.Dimethyl Sulfoxide: A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.Fertility Preservation: A method of providing future reproductive opportunities before a medical treatment with known risk of loss of fertility. Typically reproductive organs or tissues (e.g., sperm, egg, embryos and ovarian or testicular tissues) are cryopreserved for future use before the medical treatment (e.g., chemotherapy, radiation) begins.Ethylene Glycol: A colorless, odorless, viscous dihydroxy alcohol. It has a sweet taste, but is poisonous if ingested. Ethylene glycol is the most important glycol commercially available and is manufactured on a large scale in the United States. It is used as an antifreeze and coolant, in hydraulic fluids, and in the manufacture of low-freezing dynamites and resins.Tissue Preservation: The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Botany: The study of the origin, structure, development, growth, function, genetics, and reproduction of plants.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Access to Information: Individual's rights to obtain and use information collected or generated by others.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Seeds: The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.Journalism, Medical: The collection, writing, and editing of current interest material on topics related to biomedicine for presentation through the mass media, including newspapers, magazines, radio, or television, usually for a public audience such as health care consumers.European Union: The collective designation of three organizations with common membership: the European Economic Community (Common Market), the European Coal and Steel Community, and the European Atomic Energy Community (Euratom). It was known as the European Community until 1994. It is primarily an economic union with the principal objectives of free movement of goods, capital, and labor. Professional services, social, medical and paramedical, are subsumed under labor. The constituent countries are Austria, Belgium, Denmark, Finland, France, Germany, Greece, Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden, and the United Kingdom. (The World Almanac and Book of Facts 1997, p842)Cooperative Behavior: The interaction of two or more persons or organizations directed toward a common goal which is mutually beneficial. An act or instance of working or acting together for a common purpose or benefit, i.e., joint action. (From Random House Dictionary Unabridged, 2d ed)International Cooperation: The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.Biomimetic Materials: Materials fabricated by BIOMIMETICS techniques, i.e., based on natural processes found in biological systems.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Biomimetics: An interdisciplinary field in materials science, ENGINEERING, and BIOLOGY, studying the use of biological principles for synthesis or fabrication of BIOMIMETIC MATERIALS.Laboratories: Facilities equipped to carry out investigative procedures.Ice: The solid substance formed by the FREEZING of water.Antifreeze Proteins: Proteins that bind to ice and modify the growth of ice crystals. They perform a cryoprotective role in a variety of organisms.Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Climate: The longterm manifestations of WEATHER. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Advertising as Topic: The act or practice of calling public attention to a product, service, need, etc., especially by paid announcements in newspapers, magazines, on radio, or on television. (Random House Unabridged Dictionary, 2d ed)Freeze Drying: Method of tissue preparation in which the tissue specimen is frozen and then dehydrated at low temperature in a high vacuum. This method is also used for dehydrating pharmaceutical and food products.GeorgiaFreeze Fracturing: Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.Freeze Substitution: A modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low temperature.Exopeptidases: A sub-class of PEPTIDE HYDROLASES that act only near the ends of polypeptide chains.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Esophageal Neoplasms: Tumors or cancer of the ESOPHAGUS.Cell Line, Tumor: A cell line derived from cultured tumor cells.Carcinoma, Squamous Cell: A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Esophagus: The muscular membranous segment between the PHARYNX and the STOMACH in the UPPER GASTROINTESTINAL TRACT.

Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay. (1/2910)

The sperm chromatin structure assay (SCSA) is a flow cytometric (FCM) technique which exploits the metachromatic properties of Acridine Orange to monitor the susceptibility of sperm chromatin DNA to in-situ acid denaturation. SCSA was used to study the chromatin structure variations of human spermatozoa in semen, both before and after swim-up and after cryopreservation. Semen samples were provided by 19 healthy normozoospermic subjects attending pre-marriage checks. Each sample was divided into three aliquots: the first aliquot was evaluated without further treatment, the second underwent swim-up, and the third was stored according to standard cryopreservation techniques in liquid nitrogen at -196 degrees C. Samples were also analysed by light and fluorescence microscopy (after Acridine Orange staining to evaluate the number of green fluorescent sperm heads), and by computer-assisted semen analysis. The results showed that post-rise spermatozoa represent a subpopulation characterized by a general improvement of the morphological (reduction of the percentage of abnormal forms and heads, increase of the green head sperm percentage) and kinetic parameters. This subpopulation also exhibited improved chromatin structure properties, confirming that these cells have the best structural and functional characteristics, indicative of optimal fertilizing ability. On the other hand, overall sperm quality deteriorates after cryopreservation. When thawed spermatozoa underwent an additional swim-up round, a general improvement of nuclear maturity was seen in the post-rise spermatozoa.  (+info)

Arterial damage induced by cryopreservation is irreversible following organ culture. (2/2910)

OBJECTIVES: The aim of the present study was to investigate the changes which occur to the arterial wall following cryopreservation and thawing and to determine whether these changes are reversible after a week of culture in an organ bath. MATERIALS AND METHODS: Rat iliac arterial segments were cryopreserved. Once thawed, the arterial segments were cultured for a period of 0, 1, 2, 4 or 7 days. Freshly isolated rat iliac vessels cultured for 7 days served as the control group. Evaluation was made of ultrastructural changes, the expression of metalloproteinase activity (MMP-1, MMP-3 and MMP-9) and the apoptotic state of cells. RESULTS: The freezing-thawing process induced damage to the arterial segments compared to fresh control vessels. After 1 week of culture, arteries showed a high degree of tissue degeneration. Only a few individual endothelial cells remained on the luminal surface. There was a gradual increase in the proportion of apoptotic cells. The sequential expression of MMP-1 during the first 2 days and subsequent expression of MMP-3 and MMP-9 were of most significance. CONCLUSIONS: Cryopreservation induced damage to the vessels which could not be reversed by organ culture. The changes observed in the expression of metalloproteinases may be indicative of the degenerative process which occurs in the extracellular matrix.  (+info)

Effect of cryopreservation on cytochrome P-450 enzyme induction in cultured rat hepatocytes. (3/2910)

In the present study, we evaluated the inducibility of cytochrome P-450 (CYP) CYP1A, CYP2B, CYP3A, and CYP4A by beta-naphthoflavone, phenobarbital, dexamethasone, and clofibric acid, respectively, in primary hepatocyte cultures prepared from both fresh and cryopreserved rat hepatocytes. Rat hepatocytes were successfully thawed and cultured after cryopreservation in liquid nitrogen for up to 1 month. Percentage of total recovery, viable cell recovery, and final viability of the cells were 68%, 72%, and 85%, respectively. Regardless of whether they were cryopreserved or not, cultured hepatocytes exhibited near-normal morphology. Treatment of cryopreserved hepatocytes with beta-naphthoflavone caused an 8-fold increase in 7-ethoxyresorufin O-dealkylase (CYP1A1/2) activity, with an EC50 of 1.5 microM; treatment with phenobarbital caused a 26-fold increase in 7-pentoxyresorufin O-dealkylase (CYP2B1/2) activity, with an EC50 of 10 microM; treatment with dexamethasone caused a 10-fold increase in testosterone 6beta-hydroxylase (CYP3A1/2) activity, with an EC50 of 1.3 microM, whereas treatment with clofibric acid caused a 3-fold increase in lauric acid 12-hydroxylase (CYP4A1-3) activity, with an EC50 of 170 microM. The induction of CYP1A, CYP2B, CYP3A, and CYP4A enzymes by these inducers was confirmed by Western immunoblotting. The patterns of P-450 induction in cryopreserved rat hepatocytes, in terms of concentration response, reproducibility, magnitude, and specificity of response, were similar to those observed in freshly isolated hepatocytes. Additionally, the magnitude and specificity of induction was similar to that observed in vivo in rats. In conclusion, under the conditions examined, cryopreserved rat hepatocytes appear to be a suitable in vitro system for evaluating xenobiotics as inducers of P-450 enzymes.  (+info)

Binding of annexin V to plasma membranes of human spermatozoa: a rapid assay for detection of membrane changes after cryostorage. (4/2910)

When the cell membrane is disturbed, phospholipid phosphatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane. This is one of the earliest signs of apoptosis and can be monitored by the calcium-dependent binding of annexin V. Therefore, annexin V-binding, in conjunction with flow cytometry, was used to evaluate the integrity of the sperm plasma membrane after different cryostorage protocols: i.e. 10% (v/v) glycerol; sperm maintenance medium (MM); freezing medium TEST yolk buffer (TYB); or cryostorage without protection (cryoshock). Using a combination of two fluorescent dyes, annexin V and propidium iodide (PI), led to three groups of spermatozoa being identified: (i) viable spermatozoa (annexin V-negative and PI-negative); (ii) dead spermatozoa (annexin V-positive and PI-positive); and (iii) cells with impaired but integer plasma membrane (annexin V-positive and PI-negative). The percentage of vital annexin V-negative spermatozoa increased significantly (P < 0.05) from spermatozoa treated by cryoshock (15.0+/-1.2%) to spermatozoa cryopreserved by TYB (26.6+/-2.2%) via cryopreservation by 10% (v/v) glycerol (19.9+/-1.6%) and by MM (22.2 1.8%) and was associated with the percentage of motile spermatozoa (17.6+/-3.4% by glycerol; 19.6+/-3.7% by MM and 22.6+/-3.9% by TYB; P = 0.0001). Of the spermatozoa, 12-22% were annexin V-positive even though they did not bind to PI, indicating viability before as well as after cryostorage. The percentage of vital annexin V-positive spermatozoa was significantly correlated with different sperm motility parameters (velocity straight linear, r = 0.601, P = 0.018; percentage of linearly motile spermatozoa: r = 0.549, P = 0.034). We, therefore, concluded that annexin V-binding is more sensitive in detecting a deterioration of membrane functions than PI staining, and that a considerable percentage of spermatozoa might have dysfunctional plasma membranes besides dead or moribund cells. Of the cryopreservation protocols tested, TYB yielded the most viable spermatozoa. Therefore, we advocate the use of the annexin V-binding assay for the evaluation of the quality and integrity of spermatozoa.  (+info)

Freezer anthropology: new uses for old blood. (5/2910)

Archived blood fractions (plasma, settled red cells, white cells) have proved to be a rich and valuable source of DNA for human genetic studies. Large numbers of such samples were collected between 1960 and the present for protein and blood group studies, many of which are languishing in freezers or have already been discarded. More are discarded each year because the usefulness of these samples is not widely understood. Data from DNA derived from 10-35-year-old blood samples have been used to address the peopling of the New World and of the Pacific. Mitochondrial DNA haplotypes from studies using this source DNA support a single wave of migration into the New World (or a single source population for the New World), and that Mongolia was the likely source of the founding population. Data from Melanesia have shown that Polynesians are recent immigrants into the Pacific and did not arise from Melanesia.  (+info)

Preparation of endometrium for egg donation. (6/2910)

Nowadays oocyte donation is a well established method of assisted reproduction and offers the unique opportunity to treat patients with various clinical indications, with or without ovarian function, in a novel way. In women with ovarian failure, artificial menstrual cycles are required before proceeding to oocyte donation. Oestrogen may be delivered in the form of oral tablets, transdermal patches in order to bypass the gastrointestinal tract thus avoiding first pass metabolism and by vaginal application. Our regimen is oestradiol valerate given in various concentrations, in order to mimic the regular cyclic fluctuations throughout the cycle. Progesterone may be administered in the form of oral tablets, intravaginal suppositories or rings and i.m. injections. Our results, as of most other groups, strongly support the vaginal route of progesterone administration. In women with retained ovarian function, synchronization of donor-recipient cycle presents a special problem, as there is strong evidence that a temporal window of maximal endometrial receptivity exists. Cryopreservation of donated embryos may be used to overcome the problem, but this approach has the important drawback of embryonic loss occurring after freezing and thawing. The method of choice is the administration of gonadotrophin-releasing hormone agonists (GnRHa) to render the patients functionally agonadal in order to circumvent cycle asynchrony between the donor and recipient.  (+info)

Turner's syndrome and pregnancies after oocyte donation. (7/2910)

A total of 20 clinical pregnancies was achieved among 18 women with Turner's syndrome who were treated in an oocyte donation programme. The oocytes were donated by voluntary unpaid donors. A mean of 1.8 embryos per transfer was given to each recipient by way of 28 fresh and 25 frozen embryo transfers. With fresh and frozen embryos, 13 and seven pregnancies respectively were achieved. The clinical pregnancy rate per fresh embryo transfer was 46%, and the implantation rate 30%, being similar to the corresponding rates among our oocyte recipients with primary ovarian failure in general. The corresponding rates with frozen embryos were 28 and 19%. Of these pregnancies, 40% ended in miscarriage. This high rate may be explained by uterine factors. Six women were hypertensive during pregnancy, a rate comparable with that in other oocyte donation pregnancies. All these women delivered by Caesarean section. Pregnancy and implantation rates after oocyte donation were high in women with Turner's syndrome, but the risk of cardiovascular and other complications is high. Careful assessment before and during follow-up of pregnancy are important. Transfer of only one embryo at a time to avoid the additional complications caused by twin pregnancy is recommended.  (+info)

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing. (8/2910)

Uncontrolled-rate freezing techniques represent an attractive alternative to controlled-rate cryopreservation procedures which are time-consuming and require high-level technical expertise. In this study, we report our experience using uncontrolled-rate cryopreservation and mechanical freezer storage at -140 degrees C. Twenty-eight PBPC samples (10 cryovials, 18 freezing bags) from 23 patients were cryopreserved in a cryoprotectant solution composed of phosphate-buffered saline (80%, v/v) supplemented with human serum albumin (10%, v/v) and dimethylsulfoxide (10%, v/v). The cryopreservation procedure required on average 1.5 h. The mean (+/- s.e.m.) storage time of cryovials and bags was 344+/-40 and 299+57 days, respectively. Although cell thawing was associated with a statistically significant reduction of the absolute number of nucleated cells (vials: 0.3x10(9) vs. 0.2x10(9), P< or =0.02; bags: 14x10(9) vs. 11x10(9), P< or =0.0003), the growth of committed progenitors was substantially unaffected by the freezing-thawing procedure, with mean recoveries of CFU-Mix, BFU-E, and CFU-GM ranging from 60+/- 29% to 134+/-15%. Mean recoveries of LTC-IC from cryovials and bags were 262+/-101% and 155+/-27% (P< or =0.2), respectively. In 14 out of 23 patients who underwent high-dose chemotherapy and PBPC reinfusion, the pre-and post-freezing absolute numbers of hematopoietic progenitors cryopreserved in bags were compared. A significant reduction was detected for CFU-Mix (11 vs. 7.4x10(5)), but no significant loss of BFU-E (180 vs. 150x10(5)), CFU-GM (400 vs. 290x10(5)) and LTC-IC (15 vs. 16x10(5)) could be demonstrated. When these patients were reinfused with uncontrolled-rate cryopreserved PBPC, the mean number of days to reach 1x10(9)/l white blood cells and 50x10(9)/l platelets were 9 and 13, respectively. In conclusion, the procedure described here is characterized by short execution time, allows a substantial recovery of primitive and committed progenitors and is associated with prompt hematopoietic recovery following myeloablative therapy even after long-term storage.  (+info)

*Cryopreservation

... in embryo cryopreservation Ovarian tissue in ovarian tissue cryopreservation Plant seeds or shoots may be cryopreserved for ... Cryopreservation for embryos is used for embryo storage, e.g., when in vitro fertilization (IVF) has resulted in more embryos ... Cryopreservation of tissue during recent times began with the freezing of fowl sperm, which during 1957 was cryopreserved by a ... Generally, cryopreservation is easier for thin samples and small clumps of individual cells, because these can be cooled more ...

*Oocyte cryopreservation

With the first cryopreservation of sperm in 1953 and of embryos thirty years later, these techniques have become routine. Dr ... Elective oocyte cryopreservation, also known as social egg freezing, is the term used to describe non-essential egg freezing ... Oocyte cryopreservation is an option for individuals undergoing IVF who object, either for religious or ethical reasons, to the ... Human oocyte cryopreservation (egg freezing) is a procedure to preserve a woman's eggs (oocytes). This technique was mainly ...

*Semen cryopreservation

Cryopreservation of animal genetic resources § Semen Frozen bovine semen Oocyte cryopreservation Planer NEWS and Press Releases ... Semen cryopreservation (commonly called sperm banking) is a procedure to preserve sperm cells. Semen can be used successfully ... Di Santo M, Tarozzi N, Nadalini M, and Borini A (2012). "Human Sperm Cryopreservation: Update on Techniques, Effect on DNA ... Kopeika, J.; Thornhill, A.; Khalaf, Y. (2014). "The effect of cryopreservation on the genome of gametes and embryos: principles ...

*Embryo cryopreservation

... is useful for leftover embryos after a cycle of in vitro fertilisation, as patients who fail to ... Cryopreservation of embryos is the process of preserving an embryo at sub-zero temperatures, generally at an embryogenesis ... Cryopreservation of Embryos in Ethylene Glycol Freeze Media and Direct Transfer in Crossbred Cattle in the Tropics,9th ... Embryo cryopreservation is generally performed as a component of in vitro fertilization (which generally also includes ovarian ...

*Ovarian tissue cryopreservation

... is cryopreservation of tissue of the ovary of a female. Cryopreservation of ovarian tissue is ... Oktay K, Oktem O (November 2008). "Ovarian cryopreservation and transplantation for fertility preservation for medical ... Live birth after ovarian tissue autotransplantation following overnight transportation before cryopreservation. Fertil Steril. ...

*John Jain

Cryopreservation Oocyte cryopreservation KNBC.com June 15, 2005. Retrieved on August 4, 2008 Los Angeles Times, July 18, 2005. ... Jain, J.K.; Francis, M.M.; Bayrak, A.; Quinn, P.; Paulson, R.J. (2005). "Pregnancy Outcome After Cryopreservation of All ... Jain wrote an important review article on oocyte cryopreservation for the journal Fertility Sterility in 2006, identifying him ... "Oocyte cryopreservation". Fertility and Sterility. 86 (4 Suppl): 1037-46. doi:10.1016/j.fertnstert.2006.07.1478. PMID 17008147 ...

*Percutaneous epididymal sperm aspiration

Chian, Ri-Cheng (2010). Fertility Cryopreservation. Cambridge University Press. p. 52. ...

*Cryonics

Cryopreservation may be accomplished by freezing, freezing with cryoprotectant to reduce ice damage, or by vitrification to ... In London in 2016, the English High Court ruled in favor of a mother's right to seek cryopreservation of her terminally ill 14- ... Cryopreservation of humans is not reversible with present technology; cryonicists hope that medical advances will someday allow ... In the initial cryopreservation protocol, the subject is intubated and mechanically ventilated, and a highly efficient ...

*Greg Fahy

He was also Head of the Tissue Cryopreservation Section of the Transfusion and Cryopreservation Research Program of the U.S. ... More recently, he received the Cryopreservation Award from the International Longevity and Cryopreservation Summit held in ... Fahy has over thirty years of experience in the field of cryopreservation. As a scientist with the American Red Cross, he was ... Life extension Fahy GM, Wowk B, Wu J, Phan J, Rasch C, Chang A, Zendejas E (2004). "Cryopreservation of organs by vitrification ...

*21st Century Medicine

Cryobiology Cryopreservation Biobank Tissue Engineering Organ Transplantation Michael J. Taylor; Ying C. Song; Kelvin G.M. ... Gregory M. Fahy, who pioneered the use of vitrification in reproductive cryopreservation, serves on the company's Board of ... Rall WF, Fahy GM (1985). "Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification". Nature. 313 (6003): ... ISBN 0-85200-418-4. Fahy GM, MacFarlane DR, Angell CA, Meryman HT (1984). "Vitrification as an approach to cryopreservation". ...

*Dimethyl sulfoxide

"Principles of Cryopreservation". Cryopreservation and Freeze-Drying Protocols, Second Edition. Methods in Molecular Biology. ...

*Lyopreservation

It has been explored as an alternative technique for cryopreservation. The technique has the advantages of being able to ...

*Immune privilege

CS1 maint: Multiple names: authors list (link) Abazari A1, Jomha NM, Elliott JA, McGann LE (2013). "Cryopreservation of ...

*Papaverine

It is also commonly used in cryopreservation of blood vessels along with the other glycosaminoglycans and protein suspensions. ... Müller-Schweinitzer E, Hasse J, Swoboda L (1993). "Cryopreservation of human bronchi". J Asthma. 30 (6): 451-7. doi:10.3109/ ... Müller-Schweinitzer E, Ellis P (May 1992). "Sucrose promotes the functional activity of blood vessels after cryopreservation in ... Brockbank KG (February 1994). "Effects of cryopreservation upon vein function in vivo". Cryobiology. 31 (1): 71-81. doi:10.1006 ...

*KrioRus

This work is focused on improving the process of cryopreservation itself, e.g., to improve vitrification technology, or to ... Almost as many as 200 Russian citizens have entered into cryopreservation agreements with the company. It is thought that ... Prior to the companys' founding, its prospective staff members have already had some experience in cryopreservation, when in ... This is being justified as follows: "Activities, directed towards cryopreservation of people can be considered fraud if an ...

*Viability assay

Henkelman S, Lagerberg JW, Graaff R, Rakhorst G, Van Oeveren W (2010). "The effects of cryopreservation on red blood cell ... Viability assays are used to assess the success of cryopreservation techniques, the toxicity of substances, or the ... Pichugin Y, Fahy GM, Morin R (2006). "Cryopreservation of rat hippocampal slices by vitrification" (PDF). Cryobiology. 52 (2): ... Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...

*Roger Gosden

Prasath, Ethiraj B. (2008-01-01). "Ovarian tissue cryopreservation: An update". Journal of Human Reproductive Sciences. 1 (2): ... Silber, S. J. (2012-02-01). "Ovary cryopreservation and transplantation for fertility preservation". Molecular Human ... and he did important translational research on ovarian tissue cryopreservation and on ovary transplantation. Gosden was born on ... and he did important translational research on ovarian tissue cryopreservation and on ovary transplantation; his interests also ...

*Suspended animation

"Principles of Cryopreservation by Vitrification". Methods in Molecular Biology. Methods in Molecular Biology. 1257: 30-33. doi: ... "Basic principles of cryopreservation" (PDF). fao.org. "Cryogenic Storage of Human Hematopoietic Progenitor Cells" (PDF). ...

*Neuropreservation

Fahy, Gregory M.; Wowk, Brian (2015). "Principles of Cryopreservation by Vitrification". 1257: 30-33. doi:10.1007/978-1-4939- ... "Cryopreservation of the Brain: An Update" (PDF). Cryonics. http://www.alcor.org/FAQs/faq02.html Fryer, Jane (2006-07-29). "The ...

*Solid nitrogen

Porcu, Eleonora; Ciotti, Patrizia; Venturoli, Stefano (2012-12-06). Handbook of Human Oocyte Cryopreservation. Cambridge ... useful for applications such as sperm cryopreservation. The semi-solid mixture can also be called slush nitrogen or SN2. Solid ... in liquid and slush nitrogen by numerical simulation of cooling rates for French straws used for sperm cryopreservation". ...

*List of monocotyledons of Montana

2005) Biology of Plants, 7th ed., page 459 Reed, Barbara (2008). Plant cryopreservation a practical guide. New York: Springer. ...

*Cell bank

The use of cryopreservation agents is also key to the freezing process. A common cryoprotection agent used is 10% solution of ... Coopman, K (2013). Cryopreservation: Technologies, Applications and Risks/Outcomes (PDF). Nova Science Publishers. pp. 91-108. ... Rapid thaws are recommend in bringing the cells out of cryopreservation and starting up their normal metabolic processes. ... Cell banks are commonly used within fields including stem cell research and pharmaceuticals, with cryopreservation being the ...

*Evans Blue (dye)

Crutchfield A, Diller K, Brand J (1999). "Cryopreservation of Chlamydomonas reinhardtii (Chlorophyta)". European Journal of ...

*Anders Sandberg

"UK Cryonics and Cryopreservation Research Network". Retrieved 2016-02-02. Leonard, Tom (9 June 2013). "Three senior Oxford ... by signing an open letter to support research into cryonics and by being an advisor to the UK Cryonics and Cryopreservation ...

*Gregory Benford

He has also advocated human cryopreservation, for example by signing an open letter to support research into cryonics, being a ... "UK Cryonics and Cryopreservation Research Network". Retrieved 2016-02-02. Benford, Gregory (1992-08-01) [1980]. Timescape. ... member of Alcor, and by being an advisor to a UK cryonics and cryopreservation advocacy group. Gregory Benford became Emeritus ...
Embryo cryopreservation involves the generation of pre-implantation embryos using males provided by the investigator and the controlled rate cooling of collected embryos to -30°C followed by rapid freezing of embryos in liquid nitrogen to -196°C.. While embryo cryopreservation is the gold standard for preserving mouse germplasm, not all mouse strains are suitable for embryo cryopreservation. However, we have been successful at cryopreserving embryos from several different mouse strains.. Embryos for cryopreservation can be obtained in two basic ways: ...
There are many benefits of oocyte and embryo cryopreservation. When used as part of the IVF treatment process, cryopreservation can increase the likelihood of achieving pregnancy for many patients. Cryopreservation also allows couples to continue to see their families grow as IVF cycles can be repeated, years later, from the same group of eggs originally recovered for treatment.. During an IVF cycle, between 10 and 30 eggs can typically be recovered from the ovary. While it is likely that not all of these eggs will be deemed appropriate for cryopreservation, the process frequently allows couples to save a number of eggs for multiple conception attempts. In some cases, this can lead to successful treatment without the use of fertility drugs. It also reduces the likelihood that another egg retrieval procedure will be required. The number of embryos transferred depends on the number of eggs available, age, and other factors that are unique to each patient. In some cases, cryopreservation makes ...
Oocyte Cryopreservation abroad in India offers info on cost Oocyte Cryopreservation India,Oocyte Cryopreservation IVF Doctors &hospitals India.
OBJECTIVE: During IVF, non-transferred embryos are usually selected for cryopreservation on the basis of morphological criteria. This investigation evaluated an application for array comparative genomic hybridization (aCGH) in assessment of surplus embryos prior to cryopreservation. METHODS: First-time IVF patients undergoing elective single embryo transfer and having at least one extra non-transferred embryo suitable for cryopreservation were offered enrollment in the study. Patients were randomized into two groups: Patients in group A (n=55) had embryos assessed first by morphology and then by aCGH, performed on cells obtained from trophectoderm biopsy on post-fertilization day 5. Only euploid embryos were designated for cryopreservation. Patients in group B (n=48) had embryos assessed by morphology alone, with only good morphology embryos considered suitable for cryopreservation. RESULTS: Among biopsied embryos in group A (n=425), euploidy was confirmed in 226 (53.1%). After fresh single embryo
Populations of Redside Dace Clinostomus elongatus have declined in many areas across the speciesNorth American range. Therefore, the development of sperm cryopreservation technology would provide an invaluable means of preserving genetic diversity in populations that are in imminent danger of extirpation.We developed cryopreservation protocols by testing the effects of diluent (buffered sperm motility-inhibiting saline solution [BSMIS]; BSMIS + glycine; sucrose; and Hanks balanced salt solution [HBSS]), cryoprotectant (dimethyl sulfoxide [DMSO]; propylene glycol [PG]; N,N-dimethylacetamide [DMA]; and methanol), freezing rate (1, 5, and 10◦C/min), and male-to-male variation on sperm quality. Incubating sperm in extenders affected motility;BSMIS + glycine + methanol,BSMIS + glycine + PG, and HBSS + methanol were the only treatments for which motility was not significantly different from that of fresh sperm. Sperm frozen with sucrose had higher motility than sperm frozen with BSMIS + glycine, ...
Development of effective cryopreservation protocols will be essential to realizing the potential for clinical application of neural stem and progenitor cells. Current cryopreservation protocols have been largely employed in research, which does not require as stringent consideration of viability and sterility. Therefore, these protocols involve the use of serum and protein additives, which can potentially introduce contaminants, and slow cooling with DMSO/glycerol-based cryopreservation solutions, which impairs cell survival. We investigated whether serum- and protein-free vitrification is effective for functional cryopreservation of neurosphere cultures of neural stem or progenitor cells. To protect the samples from introduction of other contaminants during handling and cryostorage, an original "straw-in-straw" method (250 l sterile straw placed in 500 l straw) for direct immersion into liquid nitrogen and storing the samples was also introduced. The protocol employed brief step-wise ...
Find the best embryo cryopreservation doctors in Mumbai. Get guidance from medical experts to select embryo cryopreservation specialist in Mumbai from trusted hospitals - credihealth.com
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
The UK Cryonics and Cryopreservation Research Network is a group of UK researchers who, together with international advisors, aim to advance research in cryopreservation and its applications. Although we are a small group, we hope to promote academic and industrial activity on cryopreservation, and discuss its potential applications, including the idea of cryopreserving whole humans, commonly known as cryonics. We acknowledge that cryonics is a controversial topic, but like any unprovable approach we think its scientific discussion is necessary to permit its understanding by the public and by the wider scientific community, and it allows us to address many of the misunderstandings surrounding cryonics. We also think that cryopreservation, cryogenics and cryonics are fields with a huge potential impact on human medicine whose societal implications should be considered and debated. We hope to attract and excite students and other researchers about cryobiology, contribute to knowledge exchange and ...
Background Sperm cryopreservation has become an indispensable tool in biology. Initially, studies were aimed towards the development of efficient freezing protocols in different species that would allow for an efficient storage of semen samples for long periods of time, ensuring its viability. Nowadays, it is widely known that an important individual component exists in the cryoresistance of semen, and efforts are aimed at identifying those sperm characteristics that may allow us to predict this cryoresistance. This knowledge would lead, ultimately, to the design of optimized freezing protocols for the sperm characteristics of each male. Methodology/Principal Findings We have evaluated the changes that occur in the sperm head dimensions throughout the cryopreservation process. We have found three different patterns of response, each of one related to a different sperm quality at thawing. We have been able to characterize males based on these patterns. For each male, its pattern remained constant among
Vitrification is an ultra‐rapid cryopreservation technique that provides excellent survivability by using optimal concentrations of cryoprotectants used in a step‐wise process supporting rapid dehydration of the oocyte. The dehydrated oocyte is loaded into a thin storage device that will facilitate ultra‐rapid cooling of the oocyte when plunged into liquid nitrogen - eliminating concerns of damaging ice‐crystal formation associated with traditional slow‐freezing procedures. Thaw solutions are formulated to support rehydration through the rapid warming process that follows vitrification to optimize cellular survival.. Irvine Scientifics vitrification system is being used in hundreds of clinics worldwide to support robust cryopreservation programs that are delivering babies every day. These solutions support implantation and pregnancy rates comparable to those of fresh cycles.. ...
Ovarian tissue cryopreservation and transplantation is a real option to preserve and restore fertility in young cancer patients. However, there is a concern regarding the possible presence of malignant cells in the ovarian tissue, which could lead to recurrence of the primary disease after reimplantation. A review of the existing literature was done to evaluate the risk of transplanting malignant cells in case of the main malignant indications for ovarian tissue cryopreservation. For ovarian tissue from patients with hematologic malignancies, it is of paramount importance to identify minimal residual disease before ovarian tissue transplantation. Indeed, these pathologies, reviewed here in detail, are considered to be most at risk of ovarian metastasis ...
The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for future use.
Jenderek, M.M.; Ambruzs, B.; Tanner, J.; Holman, G.; Ledbetter, C.; Postman, J.; Ellis, D.; Leslie, C. 2014. Extending the dormant bud cryopreservation method to new tree species. In: Reed, B.M. (ed). Proceedings of the International Conference. 2. International Symposium on Plant Cryopreservation. Fort Collins (USA). 11-14 Aug 2013. Conference Paper International Society for Horticultural Science (ISHS). ISBN 978-94-62610-27-9. pp. 133-136. Acta Horticulturae. ISSN 0567-7572. no.1039 ...
CryoSearch was created by BioCision in order to help standardize the field of cryopreservation through the sharing of protocols and encouraging discussion about protocols and methods. At BioCision, our mission is to standardize sample handling. While our products help do that, products are only one part of reducing variability. The other part is how those products get used; in other words, protocols. Since we have somewhat of a specialty in cryopreservation, and there arent any other resources like CryoSearch, we thought this would be a great place to start.. Like the site? Wed love for you to check out our cryopreservation products as well. We have leak-proof and barcoded cryovials, a line of containers for slow, controlled-rate cell freezing, and tube racks that ensure precise temperature control and can also be used for snap-freezing as they are liquid-nitrogen compatible. We have a bunch of other products as well, all designed to help make your science more reliable.. Even if youre not in ...
This advisory is written for those who would like to understand the processes of mouse strain cryopreservation, why it is important and how to take advantage of the services offered. The most efficient method to protect mouse strains is by cryopreservation of gametes or embryos. Cryopreservation, and especially re-animation from frozen material, is not a trivial exercise, nor is the establishment of a cryopreservation and IVF laboratory cheap. There are numerous laboratories around the world that offer these services.
... or embryo freezing is a method used to preserve embryos by cooling and storing them at low temperatures (-196°C). They can then be thawed at a future date and transferred to the uterus, providing additional opportunity for achieving conception.. As part of the usual process of in vitro fertilization, multiple eggs may be stimulated to grow, be recovered from the ovary and become fertilized. This may result in additional embryos in excess of the number that a couple would desire to have transferred back to the uterus at one time. If the additional embryos are of sufficiently good quality to undergo the process of cryopreservation, this can be performed in order to provide another opportunity for embryo transfer. Depending on the embryo stage at the time of freezing, between 60-90% survive freeze/thaw process resulting in a future pregnancy.. If the IVF fresh embryo transfer does not result in pregnancy, the frozen embryos can be subsequently thawed and transferred to the ...
Cryopreserved donor lymphocyte infusion (DLI) products are manufactured and administered to treat relapse after allogeneic hematopoietic stem cell transplantation. Reported clinical responses to DLIs vary broadly, even within the same group of patients. While there is an implicit recognition of the fact that different manufacturing protocols may have specific effects on different cell types, cryopreservation protocols are frequently derived from our experience in the cryopreservation of stem cell products and do not account for the heterogeneous functional nature of DLI T-cell populations. Here, we report the results of a prospective, multicenter trial on the effect of four different cryopreservation solutions that were used to freeze DLIs compared to control DLIs that were refrigerated overnight ...
Abstract: There are many reasons why cryopreservation of gametes are important: 1) maintenance of genetic diversity in domestic and wild species populations (Wildt 1992; Wildt 1997; Critser and Russell 2000), 2) facilitating the distribution of "genetically superior" domestic species lines, 3) treatment of human infertility (Kuczynski et al. 2001; Ranganathan et al. 2002; Tash et al. 2003; Agarwal et al. 2004; Nalesnik et al. 2004), and 4) genetic banking of genetically modified animal models of human health and disease (Critser and Russell 2000; Knight and Abbott 2002). Although cryopreservation of gametes have been routine in many other livestock industries such as the dairy industry. Cryopreservation in the swine industry is still in it infancy. Birth of live offspring has been reported from cryopreserved sperm and embryos, but success is still extremely low. From an industry perspective the low success rate has too much of an economic impact that the integration of the technology has been ...
Taconic offers both embryo and sperm cryopreservation. Taconic also offers revitalization of cryopreserved lines. Learn more today.
An undesired side effect of cancer treatment is potential subfertility or infertility. Timely cryopreservation of semen is the best modality to ensure fertility. This retrospective data analysis established the usage rate of cryopreserved semen from cancer patients. Pubertal and post-pubertal patients who could become infertile as a result of cancer ... read more (treatment) were offered the option to cryopreserve semen prior to treatment. Of the 898 patients who cryopreserved their semen in our hospital, 96 (10.7%) used this for assisted reproductive technology. The live birth rates for intrauterine insemination, in-vitro fertilization, intracytoplasmic sperm injection and cryopreserved embryo transfer were 13%, 29%, 32% and 17%, respectively. Of all couples involved, 77% achieved parenthood, i.e. 60 of the 78 patients (with complete follow-up) fathered at least one child. show less ...
Excessive reactive oxygen species generation during sex sorting and cryopreservation of stallion sperm leads to DNA fragmentation, lipid peroxidation, and motility loss. In this study we investigated whether antioxidant supplementation during sex sorting and cryopreservation could ameliorate the effects of reactive oxygen species on stallion sperm. In experiment 1, the postthaw characteristics of stallion sperm (N = 9) cryopreserved in the presence or absence of catalase (200 U/mL), cysteine (0.2 mg/mL), or quercetin (0.15 mM) was examined. Motility and acrosome integrity were assessed at 0, 1, and 3 hours after thawing. The sperm chromatin structure assay (SCSA; detectable DNA fragmentation index [DFI], mean DFI, and DFI) was used to assess DNA integrity immediately after thawing. Quercetin increased the total postthaw motility (25.3% vs. 20.9%; P | 0.05), but there was no beneficial effect of catalase or cysteine. Based on these results, the effect of quercetin during cryopreservation on the postthaw
DALLAS, Texas (SEND2PRESS NEWSWIRE) -- Women delaying childbearing or requiring fertility preservation prior to undergoing treatment now have an option with the launch of oocyte cryopreservation at Fertility Specialists of Texas. Now there is an option - egg freezing - that enables women to have eggs (also known as oocytes) aspirated before undergoing cancer treatment, and frozen for later use. - News from Fertility Specialists of Texas, issued by Send2Press Newswire
Background Simple and effective cryopreservation of human oocytes would have an enormous impact on the financial and ethical constraints of human assisted reproduction. Recently, studies have demonstrated the potential for cryopreservation in an ice-free glassy state by equilibrating oocytes with high concentrations of cryoprotectants (CPAs) and rapidly cooling to liquid nitrogen temperatures. A major difficulty with this approach is that the high concentrations required for the avoidance of crystal formation (vitrification) also increase the risk of osmotic and toxic damage. We recently described a mathematical optimization approach for designing CPA equilibration procedures that avoid osmotic damage and minimize toxicity, and we presented optimized procedures for human oocytes involving continuous changes in solution composition. Methods Here we adapt and refine our previous algorithm to predict piecewise-constant changes in extracellular solution concentrations in order to make the predicted ...
Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request ...
... of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use.
The use of mixed microbial communities (microbiomes) for biotechnological applications has steadily increased over the past decades. However, these microbiomes are not readily available from public culture collections, hampering their potential for widespread use. The main reason for this lack of availability is the lack of an effective cryopreservation protocol. Due to this critical need, we evaluated the functionality as well as the community structure of three different types of microbiomes before and after cryopreservation with two cryoprotective agents (CPA). Microbiomes were selected based upon relevance towards applications: (1) a methanotrophic co-culture (MOB), with potential for mitigation of greenhouse gas emissions, environmental pollutants removal and bioplastics production; (2) an oxygen limited autotrophic nitrification/denitrification (OLAND) biofilm, with enhanced economic and ecological benefits for wastewater treatment, and (3) fecal material from a human donor, with potential ...
In the past two decades, laboratories around the world have produced thousands of mutant, transgenic, and wild-type zebrafish lines for biomedical research. Although slow-freezing cryopreservation of zebrafish sperm has been available for 30 years, current protocols lack standardization and yield inconsistent post-thaw fertilization rates. Cell cryopreservation cannot be improved without basic physiological knowledge, which was lacking for zebrafish sperm. The first goal was to define basic cryobiological values for wild-type zebrafish sperm and to evaluate how modern physiological methods could aid in developing improved cryopreservation protocols. Coulter counting methods measured an osmotically inactive water fraction (Vb) of 0.37+/-0.02 (SEM), an isosmotic cell volume (V(o)) of 12.1+/-0.2mum(3) (SEM), a water permeability (L(p)) in 10% dimethyl sulfoxide of 0.021+/-0.001(SEM)mum/min/atm, and a cryoprotectant permeability (P(s)) of 0.10+/-0.01 (SEM)x10(-3)cm/min. Fourier transform infrared ...
Attain higher post-thaw sperm recovery with the only CE marked and FDA approved medium with TEST-yolk buffer (TYB) and glycerol for freezing sperm.
I15 Should Cryoprotectants be Formulated According to the Mouse Genome?. Mike Legge, Mat Byers and Stephen Bird. Molecular Embryology Group, Department of Biochemistry, University of Otago, PO BOX 56 Dunedin, New Zealand. The use of penetrating cryporotectants such as dimethyl sulphoxide and I,2-propanediol is well established for mouse embryo cryopreservation. However, the sensitivity of both oocytes and embryos to adverse effects of both cryoprotectants and the freezing process is well documented (Duliquost et al. 1999). For mouse genome cryopreservation this is especially important as there is a correlation between mouse embryo genotype and cryopreservation success. (Schmidt et al. 1985). With the increasing necessity to archive mouse genomes by embryo and gamete cryopreservation the improvement in cryoprotectant formulations is becoming increasingly important. Recently we identified the non-enzymatic formation of formaldehyde in cryoprotectant solutions and that at the micro-molar ...
Mouse half ovaries were cryopreserved and orthotopically transplanted into ovariectomized recipients genetically identical to ovary donors except for the coat color gene. Fertility was reestablished in 57% of the female recipients, which became pregnant in an average of 40 days after transplantation of frozen-thawed half ovaries. These experiments demonstrate that ovary cryopreservation can be a very useful option for banking mouse germplasm, or managing subfertile animal colonies, when embryo or sperm freezing cannot be used or is not cost effective.
It is well known that laser-assisted hatching (LAH) is the most popular and ideal embryo hatching technology, but the relevance to pregnancy outcomes of cryopreserved-thawed embryo transfer (ET) is...
Bioarray offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request ...
The aim of this study was to compare the effects of cryopreservation at approximately -196 °C in liquid nitrogen (N) and freezing at approximately -20 °C in a freezer, on the viability and survival of eight different mastitogenic bacteria inoculated in milk. Bacteria were frozen at approximately -20 °C in a freezer and cryopreserved at approximately -196 °C in liquid nitrogen. An effective preservation method was needed for follow-up samples from cows identified in the South African National Milk Recording Scheme (NMRS) with somatic cell counts above 250 000 cells/mL milk. The organisation responsible for sample collection of the NMRS milk samples also provides producers with liquid nitrogen for their semen flasks at the collection sites. This existing mode of storage and transport could therefore be utilised. Ten samples of each organism were thawed and cultured bi-weekly until week 18 for both temperature treatments. An additional sampling was performed at week 30 for samples frozen at ...
Recently, Ive heard of something called Oocyte cryopreservation, where a (fertilized, I think) egg from a woman is extracted, frozen and later thawed and reinserted into the woman to delay pregnancy.. Now, this is just an idea, I dont know if this is actually possible, but can this frozen egg be implanted into a different woman, who isnt the original owner of the egg? If yes, whose genes would the child inherit? Would it get genes from all three parents, or just from the original owner of the sperm and egg?. ...
A device for use in cryopreservation of blood vessels comprising a pair of styles insertable into the ends of a dissected blood vessel segment. The styles are mountable on a support track whereby the blood vessel can be distended and supported during cryopreservation procedures. Also disclosed is a freezing and thawing profile capable of maximizing endothelial cell survival. The use of chondroitin sulfate or similar compound is discussed as a novel cryoprotectant.
Morphological changes in equine, bovine, and canine erythrocytes, when interacting with 20% dimethylsulfoxide (DMSO) and 30% polyethylene oxide with molecular mass of 1500 (PEO-1500) solutions, as well as after cell cryopreservation at the presence of the mentioned cryoprotectants have been studied in this research using light microscopy. Frozen-thawed erythrocytes of all animal species were established to be transformed into echinocytes under DMSO penetrative cryoprotectant effect and into stomatocytes under a non-penetrative one. The state of animal frozen-thawed erythrocytes when transferring them into physiological conditions was analysed.. ...
The present Core C (Cryopreservation and Stem Cell Biology Core), evolved from a predecessor devoted strictly to cryopreservation. Core C is far more sophistica...
147 Essays on Infinite Lifespans Brian Wowk 17) Fahy GM & MacFarlane DR & Angell CA & Meryman HT; Vitrification as an approach to cryopreservation in: Cryobiology (1984, Vol. 21); pg. 407 426 18) Rall WF & Fahy GM; Ice-free cryopreservation of mouse embryos at -196 degrees C by vitrification in: Nature (1985, Vol. 313); pg. 573 575 19) Song YC & Khirabadi BS & Lightfoot F & Brockbank KG & Taylor MJ; Vitreous cryopreservation maintains the function of vascular grafts in: Nature Biotechnology (2000, Vol. 18); pg. 296 299 20) Fahy GM & Wowk B & Wu J & Paynter S; Improved vitrification solutions based on the predictability of vitrifica- tion solution toxicity in: Cryobiology (in press) 21) Wowk B & Leitl E & Rasch CM & Mesbah-Karimi N & Harris SB & Fahy GM; Vitrification enhancement by synthetic ice blocking agents in: Cryobiology (2000, Vol. 40); pg. 228 236 22) Wowk B & Fahy GM; Inhibition of bacterial ice nucle- ation by polyglycerol polymers in: Cryobiology (2002, Vol. 44); pg. 1423 23) Fahy GM ...
mouse embryos Vitrification Freezing of mouse spermatozoa ICSI Freezing of oocytes Freezing of ovaries Gnotobiology Health monitoring Links Internal Site Bibliography Freezing of mouse spermatozoa Key references Landel CP Archiving mouse strains by cryopreservation Lab Anim NY 2005 34 50 7 PMID 15806091 Marschall S A Boersma and M H de Angelis 2009 Sperm cryopreservation and in vitro fertilization Methods Mol Biol 530 407 420 PMID 19266334 Marschall S Huffstadt U Balling R Hrabe de Angelis M Reliable recovery of inbred mouse lines using cryopreserved spermatozoa Mamm Genome 1999 10 773 6 PMID 10430662 Nakagata N Cryopreservation of mouse spermatozoa Mamm Genome 2000 11 572 6 PMID 10886025 Ogonuki N K Mochida H Miki K Inoue M Fray T Iwaki K Moriwaki Y Obata K Morozumi R Yanagimachi and A Ogura 2006 Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring Proc Natl Acad Sci U S A 103 13098 13103 PMID 16920794 Ostermeier ...
Tobacco BY-2 suspension cells were successfully cryopreserved by a vitrification method combined with an encapsulation technique. Cell cultures cryopreserved using the optimal conditions established in this study could be thawed and grown enough to subculture in fresh medium within 14 days. However, the vitrification method was less effective for cryopreservation of BY-2 than a simplified slow prefreezing method.. ...
The Cantabrian brown bear survives as a small remnant population in northern Spain and semen cryopreservation for future artificial insemination is one of the measures being implemented for conservation of this species. As part of this program we investigated the value of adding heat shock protein A8 (HSPA8) to media (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid-TRIS-fructose with 20% egg yolk) used for chilling and cryopreserving the spermatozoa. Semen samples from eight brown bears were obtained by electroejaculation during the breeding season. In experiment 1, we tested three concentrations of HSPA8 (0.5, 1, and 5 μg/mL) to determine whether sperm motility (computer assisted sperm analysis system) and sperm survival could be improved during refrigeration (5 °C) up to 48 hours. Results showed that sperm viability (test with propidium iodide) was improved by the addition of 0.5 and 5 μg/mL HSPA8. In experiment 2, HSPA8 was added to the cryopreservation media (6% final glycerol ...
Stem cells are the basic cells which have the capacity to develop into almost all types of cells of the body. If given correct environment and necessary stimulus, each stem cell can develop into a specialized tissue/organ. Stem cells can be stored or cryopreserved for years using cryopreservation methods.
On July 5, 2016, the Federal Circuit vacated and remanded a district court decision holding that U.S. Patent No. 7,604,929, which is directed to a…
Results: Significant reduction of the progressive motility, viability and MMP was observed by the procedure of freezing and thawing, while there was not any significant difference between both cryopreservation techniques. Cryopreservation resulted in 48% reduction of the percentage of viable spermatozoa and 54.5% rise in the percentage of dead spermatozoa. In addition, high MMP was reduced by 24% and low MMP was increased by 34.75% in response to freezing and thawing. Progressive motility of spermatozoa was correlated significantly positive with high MMP and significantly negative with low MMP in control as well as post-thawing specimens (r=0.8881/ -0.8412, 0.7461/ -0.7510 and 0.7603/ -0.7839 for control, slow and vitrification respectively, p=0.0001 ...
IV. EFFECTS OF CRYOPRESERVATION ON THE HISTOLOGY OF SELECTED TISSUES (Liver and Kidneys) Histology was evaluated in two animals each from the FIG and FIGP groups, and in one control animal. Only brain histology was evaluated in the straight-frozen control … Continue reading →. ...
Vitrification is now the preferred choice for cryopreservation of human gametes and embryos.. Clinical outcomes obtained from vitrification normally exceed those from slow freezing - and reports of comparable pregnancy and implantation rates from fresh and vitrified cases are becoming more frequent. In addition, vitrification provides the possibility to stop at any time in the process if conditions are less than perfect.. ...
One of the critical elements for the clinical availability of cells for use in immunotherapy is cryopreservation. However, cryopreserving cells relies on antiquated protocols which raise serious safety concerns. DMSO has been associated with post-thaw morphological and epigenetic changes, weakened biodistribution and acute toxicity ...
Fifty years ago, scientists performed the first-ever cryopreservation procedure on a recently-deceased human patient: James Bedford, who had died of pancreatic cancer earlier that day. As per his will, his body was frozen in liquid nitrogen within hours of his clinical death. Kept at a temperature of -196 degrees Celsius for five decades, Bedfords hope was that he could be resuscitated in the future, at a time when our technological advancements had brought about the cure for cancer and... Death itself. January 12th is celebrated by proponents of cryopreservation as Bedford Day . This year is a particularly significant milestone: since Bedford was frozen in 1967, this marks a half century since his death and subsequent preservation. Unfortunately for him, the envisioned future in which cryopreserved corpses can be brought back from the dead-and subsequently the past-has yet to materialize.
Video articles in JoVE about pregnancy outcome include Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface, Modified MicroSecure Vitrification: A Safe, Simple and Highly Effective Cryopreservation Procedure for Human Blastocysts, Accurate and Simple Evaluation of Vascular Anastomoses in Monochorionic Placenta using Colored Dye, Modeling Encephalopathy of Prematurity Using Prenatal Hypoxia-ischemia with Intra-amniotic Lipopolysaccharide in Rats, Co-Transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-Based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery, Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy, Guide Wire Assisted Catheterization and Colored Dye Injection for Vascular Mapping of Monochorionic Twin Placentas.
Efficient cryopreservation of stem cells is essential for the maintenance of consistent stem cell stocks. Many of the existing formulations of cryopreservation media rely on high percentages of poorly defined serum or albumin.
Successful pregnancy and delivery following combined treatment of In Vitro Maturation (IVM) and Testicular Sperm Extraction (TESE). (Fuchinoue K, Nakajo Y, Yagi A, Takeda M, Kyono K. Successful pregnancy and delivery following combined treatment of in vitro maturation (IVM) and testicular sperm extraction (TESE): a case report. J Assist Reprod Genet. 2004;21(10):371-3).. Jaques Donnez reporting on the first Live birth after orthotopic transplantation of cryopreserved ovarian tissue (Donnez J, Dolmans MM, Demylle D, Jadoul P, Pirard C, Squifflet J, Martinez-Madrid B, van Langendonckt A. vebirth after orthotopic transplantation of cryopreserved ovarian tissue. Lancet. 2004:364(9443):1405-10).. Gardner and colleagues performed the worlds first prospective single blastocyst trial, which showed the feasibility of SBT and in keeping high pregnancy rates (Gardner DK, Surrey E, Minjarez D, Leitz A, Stevens J, Schoolcraft WB. Single blastocyst transfer: a prospective randomized trial. Fertil Steril. ...
There are several techniques for embryo cryopreservation, and research is ongoing. Traditional methods include "slow," graduated freezing in a computerized setting, and "rapid" freezing methods, called "vitrification." Most of the data on the success of frozen embryo transfer reported in the medical literature are derived from embryos frozen with the traditional slow freeze method. Recent studies comparing vitrification versus traditional method indicate that vitrification is at least as good as, if not superior to, traditional slow freeze method.. Embryos can be frozen at different stages: pronuclear stage, cleavage stage or blastocyst stage. Depending on the stage at which embryos are frozen, freezing of the embryos can be performed anywhere from the first to the sixth day after fertilization. Generally only normally- developing embryos will be frozen. The embryos will be transferred through a series of solutions with increasing concentration of several cryoprotectants, substances known to ...
Kaczmarczyk, A. and Funnekotter, B. and Menon, A. and Phang, P.Y. and Al-Hanbali, A. and Bunn, E. and Mancera, R. 2012. Current Issues in Plant Cryopreservation, in Katov, I.I. (ed), Current Frontiers in Cryobiology, pp. 417-438. Croatia: InTech ...
Dr. Rita Bakshi offers cryopreservation, sperm freezing, embryo freezing, ovarian tissue freezing centre, clinic in Delhi, India at very low cost.
Lectures were delivered on various topics viz. Methods and techniques of cryopreservation; brood stock management and reproductive health of the broods; nutritional aspects of broodstock and their maintenance; Identifications of quality broods; Milt collection and physical evaluation; Extender preparation technique and its significance etc. More than 60 farmers, officials and entrepreneurs including some progressive farmers from Manipur, participated and showed interest in the gene banking technique. (Source: ICAR-Central Institute on Fisheries Education, Mumbai). ...
Smile Baby IVF : Provides Cryopreservation,Preserving egg/sperm at sub-zero temperature that helps in IVF treatments by Infertility Specialist in Cox Town.
Utility of cryopreserved hepatocytes suspended in serum to predict hepatic clearance in dogs and monkeys.: An in vitro-in vivo correlation analysis between obse
Caylor, R. E., Biesiot, P. M., Franks, J. S. (1994). Culture of Cobia (Rachycentron-canadum) - Cryopreservation of Sperm and Induced Spawning. Aquaculture, 125(41276), 81-92 ...
The Report Cryopreservation Equipments in Stem Cells - A Global Market Watch, 2009-2015 provides information on pricing, market analysis, shares, forecast, and company profiles for key industry participants. - MarketResearchReports.biz
The efficacy of Triladyl®, a commercial cryomedium for bull semen, in the cryopreservation of both human and animal infective trypanosomes as compared to EDTA Saline Glucose (ESG) 10% glycerol was evaluated in the current ...
Press release - Market Insights Reports - Cryopreservation Equipment in Stem Cells Market 2019 Strategic Assessment - Thermo Fisher Scientific, Charter Medicals, praxair - published on openPR.com
Damiano, C.; Caboni, E.; Frattarelli, A.; Condelo, E.; Arias, A.; Engelmann, F. -2011-Cryopreservation of fruit tree species through encapsulation-dehydration at the CRA: Fruit research Centre of Rome -ISHS 908-p. 187- ...
The Society For Venturism has received the remaining $28,000 funding needed for cryopreservation of ALS patient Aaron Winborn at Cryonics Institute, according
Preclinical experiments with allogeneic cardiosphere-derived cells11 have opened up a new treatment paradigm and have made the use of allogeneic hCPC via cell banks a more realistic proposition, assuming that cryopreserved cells retain their original characteristics and are immunologically safe. In the present study, we provide the first detailed description of T-cell responses to cryopreserved allogeneic hCPC. We show that cryopreserved hCPC retain their primitive pluripotent and early cardiac lineage-committed phenotype. Tailored immune assays showed that these cells are hypoimmunogenic because, whether under inflammatory conditions or not, they lack the costimulatory molecules CD80/CD86 required for conventional Th1 or Th2 type T-cell responses. In contrast, the hCPC express the costimulatory molecule PD-L1, which endows them with the capacity to drive significant allogeneic Treg responses and to attenuate an ongoing immune response.. Patients who experience heart failure after MI have ...
Cryopreservation increases the rate of apoptotic spermatozoa with decreased capability to fertilise oocytes. In order to optimise the fertilisation rates, especially in assisted reproduction the use of apoptotic sperms should be avoided. Early events of apoptosis in cryopreserved spermatozoa are not detectable by conventional methods. However, the surface of apoptotic spermatozoa is characterised by externalisation of phosphatidylserine (PS), which has a high affinity to Annexin V. Therefore, colloid paramagnetic Annexin-V-conjugated microbeads (AN-MB) were tested for their ability to eliminate apoptotic spermatozoa from a total of 40 fresh and in TEST yolk buffer cryopreserved semen samples which were provided by 15 healthy volunteers ...
Poster (2015, May 13). Long-term genetic and functional stability is a fundamental requirement for the maintenance of microorganisms. Cryopreservation is the preferred method for the long-term storage of many micro-organisms ... [more ▼]. Long-term genetic and functional stability is a fundamental requirement for the maintenance of microorganisms. Cryopreservation is the preferred method for the long-term storage of many micro-organisms, including cyanobacteria. The BCCM/ULC collection currently holds over 200 cyanobacterial strains as living cultures, but only 62 are maintained in a cryo-preserved state. The main limiting factors are the low levels of survival of some strains, as well as the long periods required to recover from cryopreservation, and thus the inability to rapidly deliver strains to clients.. The BRAIN-be project PRESPHOTO (preservation of photosynthetic micro-algae in the BCCM collections) (www.presphoto.ulg.ac.be) aims to improve the preservation of cyanobacterial and ...
A two-year-old girl has become the youngest person to undergo a new technique that could preserve her fertility after chemotherapy.. The girl is the youngest of 23 cancer patients, aged between two and 31 years, who took part in a pilot study to remove ovarian tissue and immature eggs for cryopreservation.. By freezing these tissues before they are exposed to the potentially fertility-reducing effects of chemotherapy, the technique could allow even very young cancer patients the possibility of having genetically related children later in life.. Professor Timothy Child, from the University of Oxford and medical director of Oxford Fertility, where the pilot is taking place, said: If were looking at fertility preservation in adolescent or pre-pubertal girls, then, around the world in general, not a lot can be done for them. In Oxford, we have taken a belt-and-braces approach.. The procedure involves a laparoscopy to remove some ovarian tissue, which is then frozen and can be transplanted in ...
British scientists have grown mature eggs from undeveloped ones in the laboratory and are currently seeking permission to fertilise them. The new research gives hope to young girls undergoing treatment for cancer that may leave them infertile. Adult women undergoing chemotherapy are offered egg freezing in case they are left infertile by the treatment. But this is not possible for children because their eggs are undeveloped. In this new research, scientists at the University of Edinburgh took tissue containing undeveloped eggs from the ovaries of adult women and grew them to maturity.. Dr Evelyn Telfer and Professor Richard Anderson, who carried out the work, have applied to the Human Fertilisation and Embryology Authority (HFEA) for a licence to fertilise these eggs. While legal and ethical permission is being considered, the researchers are assessing the health of the eggs. The researchers also took tissue samples from the ovaries of girls as young as eight before they had chemotherapy, but ...
WASHINGTON - The treatment beating back 9-year-old Dylan Hanlons cancer also may be destroying his chances of fathering his own children when he grows up.
A team of researchers have developed the first index identifying and documenting concurrent but unrelated diseases among adolescents and young adults with cancer.
The use of fetal bovine serum (FBS) as a cryopreservation supplement is not suitable for the banking of mesenchymal stem cells (MSCs) due to the risk of transmission of disease as well as xenogeneic immune reactions in the transplanted host. Here, we investigated if human serum albumin (HSA), human serum (HS), or knockout serum replacement (KSR) can replace FBS for the cryopreservation of MSCs. In addition, we examined the characteristics of MSCs after multiple rounds of cryopreservation. Human adipose-derived stem cells (ASCs) cryopreserved with three FBS replacements, 9% HSA, 90% HS, or 90% KSR, in combination with 10% dimethyl sulfoxide (Me 2 SO) maintained stem cell properties including growth, immunophenotypes, gene expression patterns, and the potential to differentiate into adipogenic, osteogenic, and chondrogenic lineages, similar to ASCs frozen with FBS ...
A piece of frozen ovarian tissue can be transplanted in the body of the woman from whom it was taken (autografting) after total treatment from a malignant condition.. When the location of transplantation is the anatomic location of the ovary the transplantation is termed orthotopic. If the tissue is replaced in a different part of the body the transplantation is termed heterotopic. In heterotopc transplantation the ovarian tissue can be grafted in a more convenient anatomic position (e.g. under the skin) which facilitates oocyte recovery from follicles developing under drug stimulation. Experimental data have shown restoration of ovarian function for a limited period. Researchers investigate the factors that are responsible for the re-establishment of the function of the transplanted ovarian tissue and the restoration of the woman?s reproductive ability. Autologous and heterologous transplantation is only recommended in special cases after the approval of specialized scientists.. ...
HepatoCells are single-use, cryopreserved cells derived from primary human hepatocytes using Corning proprietary immortalization technology. These cells exhibit the distinct morphology of mature primary hepatocytes and maintain key functional characteristics, while providing the convenience of a renewable resource.
HART IVF Fertility Clinic formerly known as The North Houston Center for Reproductive Medicine (NHCRM) is an office based fertility program offering a wide assortment of diagnostic and therapeutic options for the treatment of infertility.
AequoScreen Double Transfected Cell Line: Human recombinant 5-HT3A receptor in aequorin HEK-293 host cell.. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant channel in a functional assay.. All cell lines are tested for the absence of mycoplasma.. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.. Features:. ...
AequoScreen® Double Transfected Cell Line: Human recombinant P2Y11 receptor in aequorin 1321N1 host cell. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant receptor in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.. Features:. ...
AequoScreen® Double Transfected Cell Line: Human recombinant Dopamine D2L receptor in aequorin CHO-K1 host cell.. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant receptor in a functional assay.. All cell lines are tested for the absence of mycoplasma.. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.. Features:. ...
AequoScreen® Double Transfected Cell Line: Rat Mas-related MrgB3 receptor in aequorin CHO-K1 host cell. We provide two vials of cryopreserved cells (approximately 2.5x106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant channel in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.. Features:. ...
AequoScreen® Double Transfected Cell Lines: Adenosine, A1 subtype. Human Recombinant, in CHO-K1 host cell. Two vials of cryopreserved cells are shipped per order. A detailed technical dossier includes sequence, culture conditions and pharmacological properties of the recombinant receptor. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some products are not available in some countries. Please inquire at your local sales office for more information.. Features:. ...
AequoScreen® Double Transfected Cell Lines: Adrenergic, alpha 2B subtype. Human Recombinant, in CHO-K1 host cell. Two vials of cryopreserved cells are shipped per order. A detailed technical dossier includes sequence, culture conditions and pharmacological properties of the recombinant receptor. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some products are not available in some countries. Please inquire at your local sales office for more information.. Features:. ...
Citation: Jenderek, M.M., Ambruzs, B.D., Holman, G.E., Skogerboe, D.M., Staats, E.R., Turner, M., Ellis, D.D. 2007. Germplasm Preservation of Vegetatively-propagated Crops at the National Center for Genetic Resources Preservation. American Society for Horticultural Science, HortScience. July 16-19, 2007.Scottsdale, Arizona. 42:962. Meeting Abstract. Interpretive Summary: Out of 476,049 germpaslm accessions maintained by the USDA, ARS, National Plant Germplasm System (NPGS), ca. 30,000 are vegetaively-propagated and as such require preservation as non seed propagules. Numerous research reports demonstrated the advantages of long term storage of plant tissues in liquid nitrogen over field only maintained collections. While successful cryopreservation protocols were established for many plant species, the protocols are usually not applicable for the entire collection of a species or genus they were developed, and have to be modified for accessions not responding to the procedure. Currently, the ...
Speaking of brains. Currently, among other things, I develop software for viewing serial block-face microscopy data, on a contract. This is my private opinion, of course - and I am not a neurologist, my main specialization is graphics, I look at neurons to tune and test the software, I dont quite know what all the little bits around are - I look at a bit and Im like, what is it? And then I go to re-read description by one of other people at the project, and I am like, ohh, I think its a mitochondrion inside a dendrite. And then I wonder - why is it here? What does it matter where it is? What is this thing that connects it to the wall? Is it some weird imaging artifact? I do not claim to speak for everyone. Im doing my part which, among other things, can help to figure out how to preserve brains or how to digitize them ...
Is the exposure time of bovine embryos to ethylene glycol (EG) prior to freezing and/or after thawing critical to survival?. John F. Hasler. Efficient and efficacious cryopreservation of bovine embryos is critical to the commercial ET industry because, as shown by the most recent AETA statistics (2011), 72% of embryos were frozen following collection versus only 28% that were transferred fresh into recipients. Following the published report of Voelkel and Hu in 1992 on cryopreservation with EG, the commercial bovine ET industry rather quickly switched from glycerol to EG as the major cryoprotectant in freezing media. The overall percentage of embryos frozen in EG rose rapidly starting in 1992 and reached 97% in 2008, the last year that the AETA collected data on this specific statistic.. During the past 20 years there has been a continuing debate among ET practitioners regarding the question of whether EG is more toxic than glycerol to bovine embryos. This concern has led some practitioners to ...
New York Times: Putting Their Eggs and Hopes on Ice - These Companies Really, Really, Really Want to Freeze Your Eggs. Egg-freezing clinics are aggressively courting a new generation. Younger millennials are heeding the call. Jennifer Lannon lay, her feet propped in stirrups, on an examining table at Extend Fertility, an egg-freezing clinic in Midtown Manhattan…. Read More ». ...
An Australian team at Monash University reports on the methods used to achieve pregnancy in humans by means of in vitro fertilization and freezing of embryos prior to replacement in the womb. The researchers briefly ...
CBS Isothermal Freezers feature a patented liquid Nitrogen jacket to provide uniform storage temperatures in the -190°C range. Request a Quote!
Application Of Liquid Nitrogen Containers In Biological Experiments:the use of the cell culture process,embryo cryopreservation technology,save bacteria, vaccines, bone tissue, sperm and other substances, can achieve very good long-term storage effect.
May God rest their souls To our personal friends: Amanda Khethiwe Fesseh, March 15, 2012 (33 years) Tanya Hill Causey, October 25, 2011 (49 years) We must keep fighting for a cure! Are you a breast cancer survivor? Do you know of any woman who has been or is being treated for breast cancer,or do […]. read more ...
IV. EFFECTS OF CRYOPRESERVATION ON THE HISTOLOGY OF SELECTED TISSUES (Liver and Kidneys) Histology was evaluated in two animals each from the FIG and FIGP groups, and in one control animal. Only brain histology was evaluated in the straight-frozen control … Continue reading →. ...
For the first time ever, a fish embryo survived being frozen and thawed, which could help preserve species and repopulate oceans. For decades, researchers have successfully cryopreserved - frozen and thawed - mammal embryos and a variety of animal sperm, but scientists were unable to do the same for fish embryos. Now, with a pairing of gold nanoparticles and lasers that thaw at millions of degrees per minute, University of Minnesota scientists successfully cryopreserved zebra fish embryos.
Techniques of controlled-rate freezing are utilized that slowly cool embryos in cryoprotectant fluid ("anti-freeze" solution) from body temperature down to -196°C, at which temperature they are stored in containers of liquid nitrogen called dewars. The embryos are actually contained within special indelibly labeled plastic vials, or straws, that are sealed prior to freezing. Once frozen, they are placed inside labeled tubes attached to aluminum canes and stored in numbered canisters within the liquid nitrogen dewar. Site and label designations are stored in three separate file systems to avoid confusion and misidentification of cryopreserved embryos. When it comes time to thaw the embryos, all available identifiers of the stored specimen must match and be confirmed before thawing commences. The embryos are thawed out at room temperature, which takes about one to two minutes. However, the most critical element of the thaw procedure is not the timing but the careful dilution of the cryoprotectant ...
|div>ATCC offers media and supplements, easy-to-use cell dissociation reagents, and hassle-free cryopreservation media optimized for culturing stem cells. |/div> |div> |div> |div id=_com_1> |/div> |/div> |/div>
CryoStor™CS10 is an animal protein-free, serum-free cryopreservation medium containing 10% DMSO to prepare & preserve cells in ultra low temperatures.
From: Mike Darwin <. Date: 31 May 95 00:03:14 EDT Subject: SCI.CRYONICS BPI Tech Brief 16: Canine Brain Cryopreservation A Brief Lay-Level Summary of Biopreservations Canine Brain Cryopreservation Results by Charles Platt In the 1950s, experiments showed that the damage caused when the cells of a mammal are frozen can be reduced if the cells are first treated with a solution of glycerol. More recently, work by Leaf, Darwin, et. al. suggested that damage to cryonics patients might be further minimized if perfusion with glycerol was carefully monitored and controlled, using a solution whose concentration gradually increased during the perfusion process to a very high concentration where much less ice will form than is the case when no cryoprotectant or lower levels of cryoprotectant are used. Until now, there has been no systematic study to verify that this kind of controlled perfusion of cryonics patients really does result in less freezing damage than a simpler protocol. In particular, no ...
MC: Is Jackson struggling to keep up given such large numbers?. CL: We have over 1300 strains live on the shelf and over the years have worked to meticulously manage the supply and demand of the strains so investigators can get a jump start on their experiments. We also scale up our colony sizes for individual investigators who need a larger supply of animals than we currently may have. For strains that have low demand, those mice are available from our cryopreserved stocks. Cryopreservation involves either freezing embryos or sperm. Dr. Robert Taft at Jackson has been on the cutting edge of that technology and recently published his technique that helps recover sperm much more easily. Animals can then be recovered from cryopreserved stocks as needed.. So instead of having to super ovulate 50 or 60 females, fertilize, and bring embryos to the two cell stage for cryopreservation, all we have to do is take two males and freeze down the sperm and that particular model is completely archived. We cut ...
Tämä sähköpostiosoite on suojattu spamboteilta. Tarvitset JavaScript-tuen nähdäksesi sen.. Henkilökohtaiset sähköpostiosoitteet: ...
In a recent research, a group of scientists have found a new and effective method to acquire viable cells from cryopreserved tissue samples, which could help researchers to collect and analyse samples from different study sites to conduct more centralized research. Rheumatoid arthritis (RA) is a chronic disease that causes pain, stiffness, swelling, and limitation in the motion and function of multiple joints. Nobody knows exactly what causes Rheumatoid Arthritis; this is mainly due to imbalance in the immune system. Modern medicines are very effective in treating this arthritis. They help in reducing the pain, joint swelling and also suppress the arthritis. Though joints are the principal body parts affected by RA, inflammation can develop in other organs as well. The researchers aim to discover new, more effective ways to treat the inflammatory response that causes these chronic diseases by identifying the cell types and signals central to driving inflammation in RA patients. Before the ...
To provide a novel fertility preservation option for patients facing a fertility threatening cancer diagnosis or treatment regimen by establishing an ovarian tissue cryopreservation program. To determine if ovarian tissue cryopreservation provides women with a useful, successful option for fertility preservation. The hypothesis is that ovarian tissue cryopreservation for fertility preservation provides an alternative option for fertility preservation. ...
Cryopreservation has been extensively applied to the long-term storage of a diverse range of biological materials. However, no comprehensive study is currently available on the cryopreservation of periodontal ligament stem cell (PDLSC) sheets which have been suggested as excellent transplant materials for periodontal tissue regeneration. The aim of this study is to investigate the effect of cryopreservation on the structural integrity and functional viability of PDLSC sheets. PDLSC sheets prepared from extracted human molars were divided into two groups: the cryopreservation group (cPDLSC sheets) and the freshly prepared control group (fPDLSC sheets). The cPDLSC sheets were cryopreserved in a solution consisting of 90% fetal bovine serum and 10% dimethyl sulfoxide for 3 months. Cell viability and cell proliferation rates of PDLSCs in both groups were evaluated by cell viability assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, respectively. The multilineage
In two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25°C water for 17 s (11.6°C s-1) or in 5°C water for 60 s (3.3°C s-1). The viability of the frozen-thawed semen was measured by determining post-thaw motility and sperm membrane integrity. Two fertility trials were also conducted. There was no effect of trial or thaw rate on post-thaw Show moreIn two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25°C water for 17 s (11.6°C s-1) or in 5°C water for 60 s (3.3°C s-1). The viability of the frozen-thawed semen was measured by determining post-thaw motility and sperm membrane integrity. Two fertility trials were also conducted. There was no effect of trial or thaw rate on ...
Insemination with donor spermatozoa is an integral part of infertility treatment. For the last 3 years in our unit, intrauterine insemination with donor spermatozoa (IUID) has been used in preference to vaginal insemination. In this retrospective study, patients were offered an initial course of five single intrauterine inseminations with cryopreserved donor spermatozoa and treatment was then reviewed. A total of 389 patients received 1465 inseminations. In all, 1119 cycles were monitored using luteinizing hormone serum analyses and 346 cycles using the urine home test kits. The clinical pregnancy rate per insemination for the cycles monitored by the serum assay was 18.0% (202/1119) compared with the urine cycles (13.7%, 46/346) (P ,05). The pregnancy loss rate was not significantly different (14.4%, 29/202 and 21.7%, 10/46) (serum and urine cycles respectively). The viable clinical pregnancy rate was significantly higher (P ,03) for the serum cycles than for the cycles using the urinary ...
[The response of embryos of the Chinese mitten crab (Eriocheir sinensis) in terms of survival to cryoprotectants was investigated for basic knowledge to enhance cryopreservation success of E. sinensis embryos. Five stages of embryonic development (cleavage, blastula, gastrula, eyed stage, and heart-beating stage embryos corresponding to 7, 14, 20, 26, and 31 days post-spawning) were chosen and exposed to various cryoprotectants, namely methanol (MeOH), propylene glycol (PG), dimethyl sulfoxide (DMSO), and dimethylformamide (DMF), at three concentrations (10, 15, 20%) for an experimental period of 30 min. at culture temperature (16°C). The toxicity tolerance of E. sinensis embryos varied with developmental stage and cryoprotectant concentration. Cleavage and blastula embryos were very sensitive to cryoprotectant concentrations above 10%. Gastrula, eyed stage, and heart-beating stage embryos tolerated cryoprotectants to 20%, although a higher survival percentage was observed in heart beating
Abstract Methods are presented for the cryopreservation of a sheathed microfilaria, Brugia malayi, and an unsheathed species, Dirofilaria corynodes. The former survived best when frozen at the rate of -0.8° or -0.5°C per minute using 9% dimethyl sulfoxide (DMSO) as the cryopreservative. Approximately 52-79% of the thawed microfilariae developed to the third stage in Aedes aegypti mosquitoes versus 79% of the unfrozen specimens. For D. corynodes the optimum freezing rate was -2° or -5°C per minute, and 6% DMSO combined with 0.004 M polyvinylpyrrolidone (PVP) afforded the best cryoprotective effect. The development of thawed microfilariae in mosquitoes ranged from 22-32% versus 29% for unfrozen specimens. In general, the viability of both species of microfilaria was retained best when stored in liquid nitrogen (-196°C). The entire life cycle of B. malayi was completed in the laboratory using cryopreserved microfilariae as the initial source. The cryopreservation of Wuchereria bancrofti also is
Interindividual variability in protein expression of organic anion-transporting polypeptides (OATPs) OATP1B1, OATP1B3, OATP2B1, and multidrug resistance-linked P-glycoprotein (P-gp) or ABCB1 was quantified in frozen human livers (n = 64) and cryopreserved human hepatocytes (n = 12) by a validated liquid chromatography tandem mass spectroscopy (LC-MS/MS) method. Membrane isolation, sample workup, and LC-MS/MS analyses were as described before by our laboratory. Briefly, total native membrane proteins, isolated from the liver tissue and cryopreserved hepatocytes, were trypsin digested and quantified by LC-MS/MS using signature peptide(s) unique to each transporter. The mean ± S.D. (maximum/minimum range in parentheses) protein expression (fmol/µg of membrane protein) in human liver tissue was OATP1B1- 2.0 ± 0.9 (7), OATP1B3- 1.1 ± 0.5 (8), OATP2B1- 1 1.7 ± 0.6 (5), and P-gp- 0.4 ± 0.2 (8). Transporter expression in the liver tissue was comparable to that in the cryopreserved hepatocytes. ...
Human dental pulp stem cells cryopreserved at low passage (P2). Dental pulp stem cells can be differentiated to multiple cell types.

Ovarian Tissue Cryopreservation for Fertility Preservation - Full Text View - ClinicalTrials.govOvarian Tissue Cryopreservation for Fertility Preservation - Full Text View - ClinicalTrials.gov

Ovarian Tissue Cryopreservation for Fertility Preservation. The safety and scientific validity of this study is the ... Experimental: Ovarian tissue cryopreservation The ovarian tissue is cryopreserved and stored at Boston IVF, which is an FDA ... The hypothesis is that ovarian tissue cryopreservation for fertility preservation provides an alternative option for fertility ... To determine if ovarian tissue cryopreservation provides women with a useful, successful option for fertility preservation ...
more infohttps://clinicaltrials.gov/ct2/show/NCT01682525?recr=Open&cond=%22Fertility%22&rank=7

Cryopreservation - WikipediaCryopreservation - Wikipedia

... in embryo cryopreservation Ovarian tissue in ovarian tissue cryopreservation Plant seeds or shoots may be cryopreserved for ... Cryopreservation for embryos is used for embryo storage, e.g., when in vitro fertilization (IVF) has resulted in more embryos ... Cryopreservation of tissue during recent times began with the freezing of fowl sperm, which during 1957 was cryopreserved by a ... Generally, cryopreservation is easier for thin samples and small clumps of individual cells, because these can be cooled more ...
more infohttps://en.wikipedia.org/wiki/Cryopreservation

Oocyte cryopreservation - WikipediaOocyte cryopreservation - Wikipedia

With the first cryopreservation of sperm in 1953 and of embryos thirty years later, these techniques have become routine. Dr ... Elective oocyte cryopreservation, also known as social egg freezing, is the term used to describe non-essential egg freezing ... Oocyte cryopreservation is an option for individuals undergoing IVF who object, either for religious or ethical reasons, to the ... Human oocyte cryopreservation (egg freezing) is a procedure to preserve a womans eggs (oocytes). This technique was mainly ...
more infohttps://en.wikipedia.org/wiki/Oocyte_cryopreservation

CryopreservationCryopreservation

In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ... Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in ... Cryopreservation Tools for Microbiology ATCC has the tools you need for the successful cryopreservation of your microbial ... Cryopreservation Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires ...
more infohttp://www.atcc.org/Products/Culture_Reagents/Cryopreservation.aspx?geo_country=us

Oocyte CryopreservationOocyte Cryopreservation

... ABSTRACT: In 2013, the American Society for Reproductive Medicine and the Society for Assisted ... Oktay K, Cil AP, Bang H. Efficiency of oocyte cryopreservation: a meta-analysis. Fertil Steril 2006;86:70-80. [PubMed] [Full ... Human oocyte cryopreservation. Hum Reprod Update 2007;13:591-605. [PubMed] [Full Text] ⇦ ... Mature oocyte cryopreservation is a currently available method of fertility preservation in women of reproductive age. Although ...
more infohttps://www.acog.org/Clinical-Guidance-and-Publications/Committee-Opinions/Committee-on-Gynecologic-Practice/Oocyte-Cryopreservation

Sperm CryopreservationSperm Cryopreservation

... Service. The JAX Sperm Cryopreservation method was developed by our reproductive science experts and has ... Sperm Cryopreservation techniques were developed by JAX scientists and are an essential tool for efficient colony management. ...
more infohttps://www.jax.org/jax-mice-and-services/cryo-and-strain-donation/cryopreservation/sperm-cryopreservation

Cryopreservation of Parathyroid GlandsCryopreservation of Parathyroid Glands

... Marlon A. Guerrero Department of Surgery, The University of Arizona, 1501 N. Campbell ... M. F. Herrera, C. S. Grant, J. A. Van Heerden, D. Jacobsen, A. Weaver, and L. A. Fitzpatrick, "The effect of cryopreservation ... C. R. McHenry, D. B. Stenger, and N. K. Calandro, "The effect of cryopreservation on parathyroid cell viability and function," ... M. F. Brennan, E. M. Brown, H. F. Sears, and G. D. Aurbach, "Human parathyroid cryopreservation: in vitro testing of function ...
more infohttps://www.hindawi.com/journals/ije/2010/829540/ref/

Options for Elective CryopreservationOptions for Elective Cryopreservation

... by R. Michael Perry. [Update of the article Options for Brain-Threatening Disorders ... Resuscitation from cryopreservation is a subject that has many divergent points of view even among those who accept the basic ... Though cryonicists see cryopreservation as a medical procedure, legally it qualifies as "disposal of a dead body" (or other ... Better still would be to have cryopreservation treated as a medical procedure which could be freely chosen and started at any ...
more infohttp://www.alcor.org/Library/html/ElectiveCryopreservation.html

Human Cryopreservation Stabilization MedicationsHuman Cryopreservation Stabilization Medications

... by Aschwin de Wolf The goal of human cryopreservation standby and ... The choice of diprivan is a typical example of the sort of trade off that sometimes needs to be made in human cryopreservation ... In human cryopreservation vasopressors (pressors) are used to increase blood pressure and selectively shift blood flow to the ... Because avoiding some of the side effects of these medications is not as high a priority in human cryopreservation as in ...
more infohttp://www.alcor.org/Library/html/stabilizationmeds.html

Seed Cryopreservation of Some Medicinal LegumesSeed Cryopreservation of Some Medicinal Legumes

P. C. Stanwood, "Cryopreservation of seed germplasm for genetic conservation," in Cryopreservation of Plant Cells and Organs, K ... P. Chmielarz, "Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra," Acta Biologica Hungarica, vol. 61, no. 2, ... V. C. Pence, "Cryopreservation of seeds of Ohio native plants and related species," Seed Science and Technology, vol. 19, no. 2 ... F. Engelmann, "Plant cryopreservation: progress and prospects," In Vitro Cellular and Developmental Biology-Plant, vol. 40, no ...
more infohttps://www.hindawi.com/journals/jb/2012/186891/

Cryopreservation | Laboratory TalkCryopreservation | Laboratory Talk

Cryopreservation. Latest News. Latest developments in the freeze drying of red blood cells using biomimetic apatites. A ...
more infohttp://laboratorytalk.com/category/169/cryopreservation

Coral Reproduction and Cryopreservation | Smithsonians National ZooCoral Reproduction and Cryopreservation | Smithsonian's National Zoo

... which made it practically unsuitable for cryopreservation. With that in mind, the team studied the characteristics of the ... scientists from the Australian Institute of Marine Science and the Taronga Conservation Society in coral reef cryopreservation ...
more infohttps://nationalzoo.si.edu/center-for-species-survival/coral-reproduction-and-cryopreservation

Oocyte Cryopreservation: Paradigm in Assisted Reproduction Technology | SpringerLinkOocyte Cryopreservation: Paradigm in Assisted Reproduction Technology | SpringerLink

Cryopreservation is the branch of science in which the effects of very low temperature on living beings at cellular level are ... Massip A (2001) Cryopreservation of embryos of farm animals. Reprod Domes Anim 36(2):49-55CrossRefGoogle Scholar ... Rall WF, Fahy GM (1985) Ice-free cryopreservation of mouse embryos at −196°C by vitrification. Nature 313:573-575CrossRefPubMed ... Friedler S, Giudice LC, Lamb EJ (1988) Cryopreservation of embryos and ova. Fertil Steril 49(5):743-764CrossRefPubMedGoogle ...
more infohttps://link.springer.com/chapter/10.1007%2F978-981-10-4702-2_21

Natures antifreeze inspires revolutionary bacteria cryopreservation techniqueNature's antifreeze inspires revolutionary bacteria cryopreservation technique

Natures antifreeze inspires revolutionary bacteria cryopreservation technique *Synthetic reproductions of antifreeze proteins ... The Warwick team, led by Professor Matthew I. Gibson, has developed a new method for cryopreservation, inspired by the process ... By combining two polymers to slow ice growth during cryopreservation, the researchers were able to recover more bacteria after ... Natures antifreeze inspires revolutionary bacteria cryopreservation technique * UKs first access course in Islamic Education ...
more infohttps://warwick.ac.uk/newsandevents/pressreleases/natures_antifreeze_inspires/

Natures antifreeze inspires revolutionary bacteria cryopreservation techniqueNature's antifreeze inspires revolutionary bacteria cryopreservation technique

Natures antifreeze inspires revolutionary bacteria cryopreservation technique *Synthetic reproductions of antifreeze proteins ... The Warwick team, led by Professor Matthew I. Gibson, has developed a new method for cryopreservation, inspired by the process ... By combining two polymers to slow ice growth during cryopreservation, the researchers were able to recover more bacteria after ... Natures antifreeze inspires revolutionary bacteria cryopreservation technique * UKs first access course in Islamic Education ...
more infohttps://warwick.ac.uk/newsandevents/pressreleases/natures_antifreeze_inspires

Missouri girl undergoes cryopreservation | KBIAMissouri girl undergoes cryopreservation | KBIA

Upon death, her body was cryopreserved--frozen--and relocated to a cryopreservation facility called the Alcor Life Extension ...
more infohttp://www.kbia.org/post/missouri-girl-undergoes-cryopreservation

cryopreservation Archives - Inside Biobankingcryopreservation Archives - Inside Biobanking

Toward Better Cryopreservation: Xeno-Free, Reduced Toxicity Prot.... Biobanks play a critical role as functional components of ... animals automation best practices blood cancer collaboration conference consent cooling rate CPA cryopreservation data ... Cryopreservation requires a slow cooling process t... by Emily Humphreys / 04.25.2017 ...
more infohttps://www.thermofisher.com/blog/biobanking/tag/cryopreservation/

Recent Articles | Cryopreservation | The Scientist Magazine®Recent Articles | Cryopreservation | The Scientist Magazine®

Researchers report the first evidence of cryopreservation by an overwintering insect in which stores of an uncommon lipid are ... Researchers show that tissues are more likely than single cells to suffer damage during cryopreservation because of the tight ...
more infohttps://www.the-scientist.com/?articles.list/tagNo/1809/tags/cryopreservation/

Cryopreservation of human heart cells.Cryopreservation of human heart cells.

7316904 - Cryopreservation of avian lymphoid cells.. 11079614 - Viability of cells in cryopreserved canine cardiovascular ...
more infohttp://www.biomedsearch.com/nih/Cryopreservation-human-heart-cells/3197434.html

Cryopreservation Stock Photo & More Pictures of Animal Embryo | iStockCryopreservation Stock Photo & More Pictures of Animal Embryo | iStock

Download this Cryopreservation photo now. And search more of iStocks library of royalty-free stock images that features Animal ...
more infohttps://www.istockphoto.com/photo/cryopreservation-gm172640159-576756

Emory Cryopreservation Program Freezes and Stores EmbryosEmory Cryopreservation Program Freezes and Stores Embryos

The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and stores embryos for ... Embryo Cryopreservation The Emory Reproductive Center offers patients an outstanding Cryopreservation Program that freezes and ... The Process of Cryopreservation. At the Emory Reproductive Center, the mechanical process of embryo cryopreservation is ... Advantages of Cryopreservation. The Ethics Committee of the American Society of Reproductive Medicine has suggested the ...
more infohttps://www.emoryhealthcare.org/womens-health/cryopreservation.html

Cryopreservation - Biology-OnlineCryopreservation - Biology-Online

What should we use as medium for cryopreservation of human esophageal cancer cell line - FBS with 10% DMSO OR its own medium ...
more infohttps://www.biology-online.org/biology-forum/viewtopic.php?p=140635

Cryopreservation of Finnhorse semen - NordgenCryopreservation of Finnhorse semen - Nordgen

Three different stallion candidate lists for future cryopreservation were suggested:. *stallions born in the last generation ( ... Saija Tenhunen and Tytti Salonpää evaluated cryopreservation options of stallion semen in their bachelor theses. The population ... as well as assess which stallions would be the best candidates for cryopreservation. The candidates should be healthy, fertile ... thus ex-situ cryopreservation is the recommended. The OCS method selects candidates based on their pedigree information and ...
more infohttps://www.nordgen.org/skand/cryopreservation-of-finnhorse-semen/

Cell Culture Reagents, Cryopreservation MediaCell Culture Reagents, Cryopreservation Media

Cellvation is a unique cryopreservation medium designed for optimal cell recovery and convenience. It does not contain DMSO or ... To complete the serum-free product range, MP introduces CellVationtm, a unique cryopreservation medium which does not contain ...
more infohttps://www.mpbio.com/life-sciences/cell-biology/cell-culture-reagents/media_type_slr:cryopreservation_media

Sperm Cryopreservation - PORTAL MyHEALTHSperm Cryopreservation - PORTAL MyHEALTH

Cryopreservation of human spermatozoa: comparison of two cryopreservation methods and three cryoprotectants. Fertility and ... Sperm Cryopreservation is available for men who wish to bank sperm for future use. In Hospital Kuala Lumpur, sperm ... Sperm cryopreservation allows future use of sperm in fertility treatments such as Intra Uterine Insemination (IUI) or In Vitro ... Sperm cryopreservation (commonly called sperm banking or sperm freezing) is a procedure to preserve sperm cells. ...
more infohttp://www.myhealth.gov.my/en/sperm-cryopreservation/
  • We thus conclude that cryopreservation induces a consistent set of changes in PBMCs from both healthy and HIV-infected individuals, and that certain immunological studies of HIV-infected patients (i.e. studies of immune reconstitution following antiretroviral therapy in HIV-infected patients or studies of HIV-infectivity in vitro using CCR5-tropic strains) should be performed on fresh samples. (nih.gov)
  • In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use. (atcc.org)
  • To complete the serum-free product range, MP introduces CellVationtm, a unique cryopreservation medium which does not contain either DMSO or serum. (mpbio.com)
  • Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. (atcc.org)
  • The goal of human cryopreservation standby and stabilization procedures is to preserve the structure and viability of the brain after medico-legal pronouncement of death. (alcor.org)
  • Resuscitation from cryopreservation is a subject that has many divergent points of view even among those who accept the basic idea of cryonics. (alcor.org)
  • Cryopreservation equipment include drystore freezers, mechanical freezers, cryopreservation freezers, cryopreservation vials, incubators and stem cell research laboratory equipment. (openpr.com)
  • The Warwick team, led by Professor Matthew I. Gibson, has developed a new method for cryopreservation, inspired by the process by which organisms known as extremophiles, survive in some of the coldest regions on Earth. (warwick.ac.uk)
  • This said, conservation of the genetic diversity in the long term should be of top priority to guarantee vitality of the Finnhorse population also in the future, thus ex-situ cryopreservation is the recommended. (nordgen.org)
  • I also agree that, in the future, there will be increasing numbers of organs (and other body parts) based upon a variety of technologies for which cryopreservation will be needed. (cryonet.org)
  • The report also calculates the forthcoming status of Cryopreservation Equipment in Stem Cells market based on thorough analysis. (openpr.com)
  • Top Companies involved in the Global Cryopreservation Equipment in Stem Cells Market are Thermo Fisher Scientific, Charter Medicals, Linde Gas Cryoservices, praxair and other. (openpr.com)
  • Comprehensive assessment of all opportunities and risk in Cryopreservation Equipment in Stem Cells market. (openpr.com)
  • Cryopreservation Equipment in Stem Cells market recent innovations and major events. (openpr.com)
  • Detailed study of business strategies for growth of Cryopreservation Equipment in Stem Cells market-leading players. (openpr.com)
  • Conclusive study about the growth plot of Cryopreservation Equipment in Stem Cells market for forthcoming years. (openpr.com)
  • Finally, all aspects of the Global Cryopreservation Equipment in Stem Cells Market are quantitatively as well qualitatively assessed to study the Global as well as regional market comparatively. (openpr.com)
  • Cryopreservation of hematopoietic stem/progenitor cells for therapeutic use. (biomedsearch.com)
  • A cryonicist wishing immediate cryopreservation thus must induce a state of cardiac and respiratory arrest or clinical death-suicide in the eyes of the law-before the procedure can begin. (alcor.org)
  • Cryopreservation (storage in liquid nitrogen (LN) at −196°C) is the only currently available long-term storage technique that ensures safe conservation of genetic resources of valuable plant species [ 14 - 16 ]. (hindawi.com)
  • By combining two polymers to slow ice growth during cryopreservation, the researchers were able to recover more bacteria after freezing than using conventional methods. (warwick.ac.uk)
  • Researchers report the first evidence of cryopreservation by an overwintering insect in which stores of an uncommon lipid are critical. (the-scientist.com)
  • Researchers show that tissues are more likely than single cells to suffer damage during cryopreservation because of the tight junctions between cells. (the-scientist.com)
  • However, only few and somewhat conflicting data are presently available on the effects of cryopreservation on PBMCs, especially in samples from HIV-infected patients in which assessment of lymphocyte phenotype and function is of the outmost importance. (nih.gov)
  • The Optimal Contribution Selection (OCS) approach was used to calculate the individual genetic contributions to the current population, as well as assess which stallions would be the best candidates for cryopreservation. (nordgen.org)
  • Cellvation is a unique cryopreservation medium designed for optimal cell recovery and convenience. (mpbio.com)
  • Cryopreservation methods seek to reach low temperatures without causing additional damage caused by the formation of ice crystals during freezing. (wikipedia.org)
  • Though cryonicists see cryopreservation as a medical procedure, legally it qualifies as "disposal of a dead body" (or other remains). (alcor.org)
  • ATCC has the tools you need for the successful cryopreservation of your microbial cultures. (atcc.org)
  • Is the Subject Area "Cryopreservation" applicable to this article? (plos.org)
  • 21CM's kidney cryopreservation program has been held up because we're waiting for a new microsurgeon to join the company, which should occur before the end of the year. (cryonet.org)
  • Once we get the program up and running, we'll be aiming at the achievement of kidney cryopreservation as soon as possible. (cryonet.org)
  • Subject: Kidney Cryopreservation And Investors In msg. (cryonet.org)
  • Saija Tenhunen and Tytti Salonpää evaluated cryopreservation options of stallion semen in their bachelor theses. (nordgen.org)