AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureHumanitiesInformation ScienceHealth CareGeographicals
TomographyCryoelectron MicroscopyElectron Microscope TomographyMicroscopy, ElectronTomography, X-Ray ComputedMicroscopy, Electron, ScanningElectronsPositron-Emission TomographyElectron TransportTomography, Optical CoherenceMicroscopy, Electron, TransmissionTomography, Emission-ComputedMicroscopy, Electron, Scanning TransmissionTomography, Spiral ComputedMicroscopyTomography, OpticalHistocytochemistryMultidetector Computed TomographyAutoradiographyTomography, Emission-Computed, Single-PhotonOrganoidsStaining and LabelingHistological TechniquesElectron Probe MicroanalysisCone-Beam Computed TomographyImaging, Three-DimensionalCell BiologyImage Processing, Computer-AssistedOsmiumTomography, X-RayNucleocapsidMicroscopy, Atomic ForceFlagellaSoftwareMolecular Sequence DataIllusionsAnalytic Sample Preparation MethodsHeavy IonsHeavy Ion RadiotherapyLinear Energy TransferSpecimen HandlingAutomation, LaboratoryArchivesSuccinic AnhydridesBiological Science DisciplinesPeriodicals as TopicPubMedDirectories as TopicCongresses as TopicFellowships and ScholarshipsScienceCaliforniaArizonaHistory, 20th CenturyAttentional Blink
Tomography: Imaging methods that result in sharp images of objects located on a chosen plane and blurred images located above or below the plane.Cryoelectron Microscopy: Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.Electron Microscope Tomography: A tomographic technique for obtaining 3-dimensional images with transmission electron microscopy.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Tomography, X-Ray Computed: Tomography using x-ray transmission and a computer algorithm to reconstruct the image.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Electrons: Stable elementary particles having the smallest known negative charge, present in all elements; also called negatrons. Positively charged electrons are called positrons. The numbers, energies and arrangement of electrons around atomic nuclei determine the chemical identities of elements. Beams of electrons are called CATHODE RAYS.Positron-Emission Tomography: An imaging technique using compounds labelled with short-lived positron-emitting radionuclides (such as carbon-11, nitrogen-13, oxygen-15 and fluorine-18) to measure cell metabolism. It has been useful in study of soft tissues such as CANCER; CARDIOVASCULAR SYSTEM; and brain. SINGLE-PHOTON EMISSION-COMPUTED TOMOGRAPHY is closely related to positron emission tomography, but uses isotopes with longer half-lives and resolution is lower.Electron Transport: The process by which ELECTRONS are transported from a reduced substrate to molecular OXYGEN. (From Bennington, Saunders Dictionary and Encyclopedia of Laboratory Medicine and Technology, 1984, p270)Tomography, Optical Coherence: An imaging method using LASERS that is used for mapping subsurface structure. When a reflective site in the sample is at the same optical path length (coherence) as the reference mirror, the detector observes interference fringes.Microscopy, Electron, Transmission: Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.Tomography, Emission-Computed: Tomography using radioactive emissions from injected RADIONUCLIDES and computer ALGORITHMS to reconstruct an image.Microscopy, Electron, Scanning Transmission: A type of TRANSMISSION ELECTRON MICROSCOPY in which the object is examined directly by an extremely narrow electron beam scanning the specimen point-by-point and using the reactions of the electrons that are transmitted through the specimen to create the image. It should not be confused with SCANNING ELECTRON MICROSCOPY.Tomography, Spiral Computed: Computed tomography where there is continuous X-ray exposure to the patient while being transported in a spiral or helical pattern through the beam of irradiation. This provides improved three-dimensional contrast and spatial resolution compared to conventional computed tomography, where data is obtained and computed from individual sequential exposures.Microscopy: The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.Tomography, Optical: Projection of near-IR light (INFRARED RAYS), in the 700-1000 nm region, across an object in parallel beams to an array of sensitive photodetectors. This is repeated at various angles and a mathematical reconstruction provides three dimensional MEDICAL IMAGING of tissues. Based on the relative transparency of tissues to this spectra, it has been used to monitor local oxygenation, brain and joints.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Multidetector Computed Tomography: Types of spiral computed tomography technology in which multiple slices of data are acquired simultaneously improving the resolution over single slice acquisition technology.Autoradiography: The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)Tomography, Emission-Computed, Single-Photon: A method of computed tomography that uses radionuclides which emit a single photon of a given energy. The camera is rotated 180 or 360 degrees around the patient to capture images at multiple positions along the arc. The computer is then used to reconstruct the transaxial, sagittal, and coronal images from the 3-dimensional distribution of radionuclides in the organ. The advantages of SPECT are that it can be used to observe biochemical and physiological processes as well as size and volume of the organ. The disadvantage is that, unlike positron-emission tomography where the positron-electron annihilation results in the emission of 2 photons at 180 degrees from each other, SPECT requires physical collimation to line up the photons, which results in the loss of many available photons and hence degrades the image.Organoids: An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Histological Techniques: Methods of preparing tissue for examination and study of the origin, structure, function, or pathology.Electron Probe Microanalysis: Identification and measurement of ELEMENTS and their location based on the fact that X-RAYS emitted by an element excited by an electron beam have a wavelength characteristic of that element and an intensity related to its concentration. It is performed with an electron microscope fitted with an x-ray spectrometer, in scanning or transmission mode.Cone-Beam Computed Tomography: Computed tomography modalities which use a cone or pyramid-shaped beam of radiation.Imaging, Three-Dimensional: The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object.Cell Biology: The study of the structure, behavior, growth, reproduction, and pathology of cells; and the function and chemistry of cellular components.Image Processing, Computer-Assisted: A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.Osmium: Osmium. A very hard, gray, toxic, and nearly infusible metal element, atomic number 76, atomic weight 190.2, symbol Os. (From Dorland, 28th ed)Tomography, X-Ray: Tomography using x-ray transmission.Nucleocapsid: A protein-nucleic acid complex which forms part or all of a virion. It consists of a CAPSID plus enclosed nucleic acid. Depending on the virus, the nucleocapsid may correspond to a naked core or be surrounded by a membranous envelope.Microscopy, Atomic Force: A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.Flagella: A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Software: Sequential operating programs and data which instruct the functioning of a digital computer.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Illusions: The misinterpretation of a real external, sensory experience.Analytic Sample Preparation Methods: Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.Heavy Ions: Positively-charged atomic nuclei that have been stripped of their electrons. These particles have one or more units of electric charge and a mass exceeding that of the Helium-4 nucleus (alpha particle).Heavy Ion Radiotherapy: The use of a heavy ion particle beam for radiotherapy, such as the HEAVY IONS of CARBON.Linear Energy Transfer: Rate of energy dissipation along the path of charged particles. In radiobiology and health physics, exposure is measured in kiloelectron volts per micrometer of tissue (keV/micrometer T).Specimen Handling: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.Automation, Laboratory: Controlled operations of analytic or diagnostic processes, or systems by mechanical or electronic devices.ArchivesSuccinic Anhydrides: A subclass of anhydrides with the general structure of dihydrofurandione. They can be substituted on any carbon atom. They modify and inhibit proteins and enzymes and are used in the acylation of amino- and hydroxyl groups.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Congresses as Topic: Conferences, conventions or formal meetings usually attended by delegates representing a special field of interest.Fellowships and Scholarships: Stipends or grants-in-aid granted by foundations or institutions to individuals for study.Science: The study of natural phenomena by observation, measurement, and experimentation.CaliforniaArizonaHistory, 20th Century: Time period from 1901 through 2000 of the common era.Attentional Blink: Temporary visual deficit or impaired visual processing occurring in a rapid serial visual presentation task. After a person identifies the first of two visual targets, the ability to detect the second target is impaired for the next few hundred milliseconds. This phenomenon is called attentional blink.
AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureHumanitiesInformation ScienceHealth CareGeographicals
TomographyCryoelectron MicroscopyElectron Microscope TomographyMicroscopy, ElectronTomography, X-Ray ComputedMicroscopy, Electron, ScanningElectronsPositron-Emission TomographyElectron TransportTomography, Optical CoherenceMicroscopy, Electron, TransmissionTomography, Emission-ComputedMicroscopy, Electron, Scanning TransmissionTomography, Spiral ComputedMicroscopyTomography, OpticalHistocytochemistryMultidetector Computed TomographyAutoradiographyTomography, Emission-Computed, Single-PhotonOrganoidsStaining and LabelingHistological TechniquesElectron Probe MicroanalysisCone-Beam Computed TomographyImaging, Three-DimensionalCell BiologyImage Processing, Computer-AssistedOsmiumTomography, X-RayNucleocapsidMicroscopy, Atomic ForceFlagellaSoftwareMolecular Sequence DataIllusionsAnalytic Sample Preparation MethodsHeavy IonsHeavy Ion RadiotherapyLinear Energy TransferSpecimen HandlingAutomation, LaboratoryArchivesSuccinic AnhydridesBiological Science DisciplinesPeriodicals as TopicPubMedDirectories as TopicCongresses as TopicFellowships and ScholarshipsScienceCaliforniaArizonaHistory, 20th CenturyAttentional Blink
Cryogenic electron microscopyCryogenic electron microscopy
Scanning electron cryomicroscopy (cryoSEM), is scanning electron microscopy technique with a scanning electron microscope's ... Electron tomography. References[edit]. *^ Cheng Y, Grigorieff N, Penczek PA, Walz T (April 2015). "A primer to single-particle ... Cryogenic electron microscopy (cryo-EM) is an electron microscopy (EM) technique applied on samples cooled to cryogenic ... Transmission electron cryomicroscopy[edit]. Main article: Transmission electron cryomicroscopy. Transmission electron ...
Cryo-electron tomographyCryo-electron tomography
Samples are imaged in a transmission electron microscope (TEM). As in other electron tomography techniques, the sample is ... ice and imaged under cryogenic conditions (< −150 °C), allowing them to be imaged without dehydration or chemical fixation, ... Electron cryo-tomography (ECT, also called cryo-electron tomography, cryo-ET or CET) is an imaging technique used to produce ... In contrast to other electron tomography techniques, samples are immobilized in non-crystalline ("vitreous") ...
CubosomeCubosome
... from a self-assembled lipid mixture and studied by means of high-resolution cryogenic transmission electron microscope (cryo- ... "Direct visualization of dispersed lipid bicontinuous cubic phases by cryo-electron tomography". Nature.com. Nature ...
Atom probeAtom probe
... s are unlike conventional optical or electron microscopes, in that the magnification effect comes from the ... Modern day atom probe tomography (APT) uses a position-sensitive detector to deduce the lateral location of atoms. The idea of ... After introduction into the vacuum system, the sample is reduced to cryogenic temperatures (typically 20-100 K) and manipulated ... so unlike for conventional electron microscopes, there is often little direct control on magnification, and furthermore, ...
ScintillatorScintillator
The holes associated with electrons in the conduction band are independent from the latter. Those holes and electrons are ... The scintillations produced by the screen were visible to the naked eye if viewed by a microscope in a darkened room; the ... Mikhailik, V. B.; Kraus, H. (2010). "Scintillators for cryogenic applications; state-of-art". Journal of Physical Studies. 14 ( ... computed tomography and gas exploration. Other applications of scintillators include CT scanners and gamma cameras in medical ...
Amira (software)Amira (software)
"Evidence of direct cell-cell fusion in Borrelia by cryogenic electron tomography". Cellular Microbiology. Wiley Online Library ... Of The Airways And Lungs For The FABP/CFTR-Knockout Mouse Using Micro-Computed Tomography And Large Image Microscope Array" ( ... on out-of-core data Anatomy Biochemistry Biophysics Cellular microbiology Computational fluid dynamics Cryo-electron tomography ... Specific readers for microscopy data Image deconvolution Exploration of 3D imagery obtained from virtually any microscope ...
InterferometryInterferometry
... with electrons,[11][12] and with buckyball molecules large enough to be seen under an electron microscope.[13] ... optical coherence tomography Optical coherence tomography (OCT) is a medical imaging technique using low-coherence ... cryogenic optical resonators Figure 8. Fourier transform spectroscopy Figure 9. A picture of the solar corona taken with the ... Electron holography is an imaging technique that photographically records the electron interference pattern of an object, which ...
Index of physics articles (C)Index of physics articles (C)
Search Cryogenic Rare Event Search with Superconducting Thermometers Cryogenic particle detectors Cryogenic processor Cryogenic ... Core electron Core-mantle boundary Coriolis effect Coriolis field Coriolis-Stokes force Corium (nuclear reactor) Cornelis Dirk ... Conductivity of transparency Conductor gallop Cone beam computed tomography Configuration interaction Configuration space ... sensor Catapult effect Catastrophic optical damage Catherine Bréchignac Cathodoluminescence Cathodoluminescence microscope ...
Confocal microscopyConfocal microscopy
... especially the microscope objective) in a cryogenic storage dewar.[13] This second approach, although more cumbersome, ... obtained is best explained by comparing it with another scanning technique like that of the scanning electron microscope (SEM ... "Confocal X-ray Fluorescence Imaging and XRF Tomography for Three Dimensional Trace Element Microanalysis". Microscopy and ... The Tandem-Scanning-Microscope[edit]. Scheme of Petráň's Tandem-Scanning-Microscope. Red bar added to indicate the Nipkow-Disk. ...
TomographyTomography
Cryogenic electron tomography Cryogenic transmission electron microscopy CryoET Electrical capacitance tomography Electrical ... Optical diffusion tomography Absorption of light ODT Optical projection tomography Optical microscope OPT ... Electrical impedance tomography Electrical impedance EIT 1984 Electron tomography Transmission electron microscopy ET 1968[6][7 ... Positron emission tomography Positron emission PET Positron emission tomography - computed tomography Positron emission & X-ray ...
AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureHumanitiesInformation ScienceHealth CareGeographicals
TomographyCryoelectron MicroscopyElectron Microscope TomographyMicroscopy, ElectronTomography, X-Ray ComputedMicroscopy, Electron, ScanningElectronsPositron-Emission TomographyElectron TransportTomography, Optical CoherenceMicroscopy, Electron, TransmissionTomography, Emission-ComputedMicroscopy, Electron, Scanning TransmissionTomography, Spiral ComputedMicroscopyTomography, OpticalHistocytochemistryMultidetector Computed TomographyAutoradiographyTomography, Emission-Computed, Single-PhotonOrganoidsStaining and LabelingHistological TechniquesElectron Probe MicroanalysisCone-Beam Computed TomographyImaging, Three-DimensionalCell BiologyImage Processing, Computer-AssistedOsmiumTomography, X-RayNucleocapsidMicroscopy, Atomic ForceFlagellaSoftwareMolecular Sequence DataIllusionsAnalytic Sample Preparation MethodsHeavy IonsHeavy Ion RadiotherapyLinear Energy TransferSpecimen HandlingAutomation, LaboratoryArchivesSuccinic AnhydridesBiological Science DisciplinesPeriodicals as TopicPubMedDirectories as TopicCongresses as TopicFellowships and ScholarshipsScienceCaliforniaArizonaHistory, 20th CenturyAttentional Blink
Visualizing Cell Architecture and Molecular Location Using Soft X-Ray Tomography and Correlated Cryo-Light MicroscopyVisualizing Cell Architecture and Molecular Location Using Soft X-Ray Tomography and Correlated Cryo-Light Microscopy
18) created the worlds first cryogenic light microscope capable of operation at high numerical aperture. Integration of this ... Baumeister W. Electron tomography: towards visualizing the molecular organization of the cytoplasm. Curr. Opin. Struct. Biol. ... Cryogen(ic). typically temperatures below −160°C. Soft X-rays. X-rays with energies less than 1 keV. Tomography. a method for ... Soft X-ray tomography and cryogenic light microscopy: the cool combination in cellular imaging. Trends Cell Biol. 2009;19:587- ...
pubmedcentralcanada.ca
Integrative Structural Biology | Thermo Fisher ScientificIntegrative Structural Biology | Thermo Fisher Scientific
... and cryo-electron microscopy (cryo-EM)-an approach known as integrative structural biology. ... Besides SPA, Cryo-electron tomography and sub-tomogram averaging result in particle structures at lower resolution, however ... The Thermo Scientific™ Talos™ Arctica is a 200kV FEG Transmission and Scanning Electron Microscope (S/TEM). It is a powerful, ... Its highly stable 300 kV TEM platform and industry-leading Autoloader (cryogenic sample manipulation robot) are designed for ...
fei.com
Single Particle Analysis Workflow, The Challenges of Protein Complex Analysis | Thermo Fisher ScientificSingle Particle Analysis Workflow, The Challenges of Protein Complex Analysis | Thermo Fisher Scientific
Comparison of the cryoEM pore complex to the prepore structure obtained by electron cryo-tomography and the x-ray structure of ... The Talos Arctica is a 200kV FEG Transmission and Scanning Electron Microscope (STEM). It is a powerful, stable, and versatile ... It features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot) and the same innovative ... The Thermo Scientific™ Talos™ Arctica is a 200kV FEG Transmission and Scanning Electron Microscope (S/TEM). It is a powerful, ...
fei.com
Krios G3i Cryo-TEM for Life Sciences | Thermo Fisher ScientificKrios G3i Cryo-TEM for Life Sciences | Thermo Fisher Scientific
Its highly stable 300 kV TEM platform and industry-leading Autoloader (cryogenic sample manipulation robot) are designed for ... automated applications, such as single particle analysis (SPA) and cryo-tomography. ... G3i Cryo Transmission Electron Microscope (Cryo-TEM) enables life science researchers to unravel life at the molecular level- ... Transmission Electron Microscopes. Krios G3i Cryo-TEM for Life Sciences. Unravelling Life at the Molecular Level - Easier, ...
fei.com
Cryo-Tomography, In Situ Structural Biology Workflow will expose the Molecular Machinery of Cells | Thermo Fisher ScientificCryo-Tomography, In Situ Structural Biology Workflow will expose the Molecular Machinery of Cells | Thermo Fisher Scientific
... and high-resolution cryo-electron tomography (Cryo-ET). ... Cryo Transmission Electron Microscope (Cryo-TEM) delivers a ... It features 200 kV XFEG optics, the industry-leading Autoloader (cryogenic sample manipulation robot) and the same innovative ... Tomography 4.0 software. The fast, easy and complete solution for electron tomography ... Step four: Cryo-TEM Tomography. A series of 2D projection images is captured using very low electron dose.. ...
fei.com
Staff Scientist for VIB-VUB facility for Bio Electron Cryogenic Microscopy (BECM) - EPSOStaff Scientist for VIB-VUB facility for Bio Electron Cryogenic Microscopy (BECM) - EPSO
BECM is a state-of-the-art cryo-EM facility equipped with JEOL cryoARM300 electron microscope with cold FEG, in-column energy ... center in single-particle cryo-EM and cryo-tomography projects. ... Staff Scientist for VIB-VUB facility for Bio Electron Cryogenic ... Home » Staff Scientist for VIB-VUB facility for Bio Electron Cryogenic Microscopy (BECM) ... in Brussels seeks to recruit a staff scientist to support operation of the VIB-VUB Bio Electron Cryogenic Microscopy (BECM) ...
epsoweb.org
Atom Probe Tomography | Max-Planck-Institut für Eisenforschung GmbHAtom Probe Tomography | Max-Planck-Institut für Eisenforschung GmbH
... of a world unique setup allowing for controlled transport of APT specimens from a glovebox to a scanning-electron microscope / ... Transport of specimens can also be performed at cryogenic temperatures. This infrastructure was funded jointly by the BMBF and ... The Atom Probe Tomography Group focuses on applying this burgeoning microscopy and microanalysis technique to a wide range of ... Atom Probe Tomography (APT) is a high-resolution characterization technique that provides three-dimensional elemental mapping ...
mpie.de
Cryo-electron tomography of cells: connecting structure and function | SpringerLinkCryo-electron tomography of cells: connecting structure and function | SpringerLink
Cryo-electron tomography (cryo-ET) allows the visualization of cellular structures under close-to-life conditions and at ... Mastronarde DN (2005) Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152 ... Also, samples are kept and imaged in EM at cryogenic temperatures to avoid devitrification. ... Cryo-electron tomography Correlative light microscopy Electron microscopy Histochemistry and Cell Biology Lecture presented at ...
link.springer.com
Sperm Cell Tails Have Helix Structures, Could Help With InfertilitySperm Cell Tails Have Helix Structures, Could Help With Infertility
Cryogenic electron tomography combines electron microscopy with CT scans. In a cryo-ET study, a biological sample, such as a ... Then, the sample is thinned out and imaged with an electron microscope. Freezing the sample ensures that it stays hydrated and ... But this knowledge gap could shrink, with a new imaging technique called cryogenic electron tomography. Also known as cryo-ET, ...
nationalgeographic.com
Cryogenic electron microscopy - WikipediaCryogenic electron microscopy - Wikipedia
Scanning electron cryomicroscopy (cryoSEM), is scanning electron microscopy technique with a scanning electron microscopes ... Electron tomography. References[edit]. *^ Cheng Y, Grigorieff N, Penczek PA, Walz T (April 2015). "A primer to single-particle ... Cryogenic electron microscopy (cryo-EM) is an electron microscopy (EM) technique applied on samples cooled to cryogenic ... Transmission electron cryomicroscopy[edit]. Main article: Transmission electron cryomicroscopy. Transmission electron ...
en.wikipedia.org
Northwestern University and Argonne National Lab Using Breakthrough Xradia Microscopy Solution to Advance Life Science Research...Northwestern University and Argonne National Lab Using Breakthrough Xradia Microscopy Solution to Advance Life Science Research...
The product range includes light and laser scanning microscopes, X-ray microscopes, electron and ion microscopes and ... computed tomography (CT), visible light microscopy and electron microscopy, often using different samples and different ... "The Bionanoprobes cryogenic sample-handling system allows researchers to move the same cryogenically preserved sample from the ... "Traditionally, looking at tissue under a regular microscope then moving to an electron microscope requires that we use ...
xradia.com
Structural Biochemistry/Imaging cellular architecture with X-rays - Wikibooks, open books for an open worldStructural Biochemistry/Imaging cellular architecture with X-rays - Wikibooks, open books for an open world
Soft x-ray tomography is in a sense similar to light and electron microscopes in that it can also generate two-dimensional ... As stated before, it is crucial that specialized cryogenic tomography stages occur during this process of X-ray imaging. A key ... There are three main broad categories of tomography - full-field, projection tomography and confocal tomography. Full-field ... Hard X-ray Fluorescence Tomography[edit]. Hard X-ray Fluorescence Tomography was a method originally created to detect the ...
en.wikibooks.org
Cryogenic SamplesCryogenic Samples
... setup of an x-ray tomography stage using linear stages and 360 degree rotation stages from PI allows to investigate cryogenic ... Electron Microscopy. Electron Microscopy. Nano precise positioning of samples is essential in all TEM and SEM microscopes in ... IsoView Light Sheet Microscope. IsoView Light Sheet Microscope. Light Sheet Microscopy is a fascinating technology with a huge ... Optical Coherence Tomography. Piezo Technology and Optical Coherence Tomography (OCT). Piezo actuators and drives, e.g., PILine ...
physikinstrumente.com
Real-time cryo-electron microscopy data preprocessing with Warp | Nature MethodsReal-time cryo-electron microscopy data preprocessing with Warp | Nature Methods
... data from biological specimens must be tightly coupled to data preprocessing to ensure the best data quality and microscope ... The acquisition of cryo-electron microscopy (cryo-EM) ... Mastronarde, D. N. Automated electron microscope tomography ... McLeod, R. A., Kowal, J., Ringler, P. & Stahlberg, H. Robust image alignment for cryogenic transmission electron microscopy. J ... Hagen, W. J. H., Wan, W. & Briggs, J. A. G. Implementation of a cryo-electron tomography tilt-scheme optimized for high ...
nature.com
Transmission Electron Microscopes | Aalto UniversityTransmission Electron Microscopes | Aalto University
TEM and STEM tomography; and in situ observation of samples. ... Yes, cryogenic liquid-nitrogen cooled sample holder. Contact. [ ...
aalto.fi
Fireside Chat: Jensen Huang from NVIDIA on how AI will revolutionize Medicine - insideHPCFireside Chat: Jensen Huang from NVIDIA on how AI will revolutionize Medicine - insideHPC
RELION is the imaging software of the Nobel Prize-winning cryogenic electron microscope. Cryo-EM lets researchers freeze ... Invented 15 years earlier, iterative reconstruction was a new algorithm in computed tomography that promised X-ray dose ...
insidehpc.com
Buy Hydroxyzine OnlineBuy Hydroxyzine Online
Argon broad ion beam tomography in a cryogenic scanning electron microscope: a novel tool for the investigation of ... Electron-density maps for the crystal structures of membrane proteins often show features suggesting binding of lipids and/or ... Multiple acyl-CoA-dehydrogenase deficiency (MADD)--a novel mutation of electron-transferring-flavoprotein dehydrogenase ETFDH. ... the present study is to perform detailed three-dimensional measurements of individual corneocytes with atomic force microscope ...
termsreign.ga
Cryo-electron tomography - WikipediaCryo-electron tomography - Wikipedia
Samples are imaged in a transmission electron microscope (TEM). As in other electron tomography techniques, the sample is ... ice and imaged under cryogenic conditions (< −150 °C), allowing them to be imaged without dehydration or chemical fixation, ... Electron cryo-tomography (ECT, also called cryo-electron tomography, cryo-ET or CET) is an imaging technique used to produce ... In contrast to other electron tomography techniques, samples are immobilized in non-crystalline ("vitreous") ...
en.wikipedia.org
JEOL USA Tomography SolutionJEOL USA Tomography Solution
Core product groups include electron microscopes (SEMs and TEMs), instruments for the semiconductor industry (electron beam ... JEOL is a world leader in electron optical equipment and instrumentation for high-end scientific and industrial research and ... Cryogenic Probes for NMR. *Liquids/Solution State Probes. *AutoMAS Solids Probe. *HCN MAS and HXY NMR Probes ... Scanning Electron Microscopes (SEM). Benchtop: NeoScope. HV/LV Tungsten/LaB6 SEMs: IT200 , IT500. High-Res, Large-Chamber SEM: ...
jeolusa.com
Tulane University - CIF Microscopy LabTulane University - CIF Microscopy Lab
The Tecnai G2 F30 TWIN is a 300 kV / FEG Transmission Electron Microscope. It is a high-resolution microscope optimized for ... The Tecnai G2 TEMs are equipped with Gatan cryogenic TEM system with FEI low dose capability, the most advanced SDD EDS system ... complete and versatile performance with high-tilt applications such as diffraction experiments and tomography for a cross- ... Scanning Electron Microscopy. A variable pressure Hitachi 3400 electron microscope was installed in 2005. ...
www2.tulane.edu
Electron Microscopy - Instruct-ERICElectron Microscopy - Instruct-ERIC
There are two main types of electron microscopes, the scanning electron microscope and the transmission electron microscope. ... Electron tomography. Electron tomography can nowadays be used for quasi in vivo studies of non-repetitive structures, such as ... Cryogenic electron microscopy. Over the past decade, cryogenic electron microscopy (cryo-EM) has increasingly replaced the ... Transmission electron microscopy. A transmission electron microscope (TEM) uses a technique whereby a beam of electrons is ...
structuralbiology.eu
Integrated Cryo-electron-Tomography Workflow | Thermo Fisher Scientific - USIntegrated Cryo-electron-Tomography Workflow | Thermo Fisher Scientific - US
... electron microscope for thinning. The Aquilos Cryo-FIB combines a scanning electron beam (SEM) and a focused ion beam (FIB). ... The cryogenic freezing process is so fast that liquid water forms non-crystalline vitreous ice, thus avoiding the damage caused ... cryo-transmission electron microscope), where the actual tomographic image acquisition takes place. The images in the ... Sample thinning is essential for the tomography workflow because the electron beam in the TEM can only pass through samples ...
thermofisher.com
High resolution (cryo-)TEM - WURHigh resolution (cryo-)TEM - WUR
Transmission Electron Microscopy has many applications across a multitude of industry sectors. It allows 2D and 3D-imaging of ... Wageningen Electron Microscopy Center The Transmission Electron Microscopes and sample preparation facilities are embedded in ... Tomography Tomography data collection program SerialEM can be used to automate acquisition of tilt-series. These EM tilt series ... The machine accommodates measurements under cryogenic temperatures, using the available cryo-holder and sample preparation ...
wur.nl
Dr. T. Rajagopalan | Amrita Vishwa VidyapeethamDr. T. Rajagopalan | Amrita Vishwa Vidyapeetham
Cryogenic transmission electron microscope (cryo-TEM) and scanning electron microscope (SEM) were employed to image the aqueous ... electron tomography and HR-SEM, is highlighted in this review. More »». ... between the bundled nanowires has been accomplished by high-resolution transmission electron microscope (HRTEM). Cryogenic-TEM ... Cryogenic-transmission electron microscopy and thermogravimetric analysis techniques are used for the characterization of these ...
amrita.edu
FIB | Imaging & Microscopy - Research, Development, ProductionFIB | Imaging & Microscopy - Research, Development, Production
A Universal Scanning Electron Microscope. In the last years SEM & FIB equipment with analytical add-ons has followed a trend of ... Focused Ion Beam / Scanning Electron Microscopy tomography is an unique method for the 3D description of different materials on ... is the first institution to obtain a novel FEI Scios dual-beam microscope, with an adaptation for use at cryogenic temperatures ... Microscopes dont exactly lie, but their limitations affect the truths they can tell. For example, scanning electron ...
imaging-git.com
AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureHumanitiesInformation ScienceHealth CareGeographicals
TomographyCryoelectron MicroscopyElectron Microscope TomographyMicroscopy, ElectronTomography, X-Ray ComputedMicroscopy, Electron, ScanningElectronsPositron-Emission TomographyElectron TransportTomography, Optical CoherenceMicroscopy, Electron, TransmissionTomography, Emission-ComputedMicroscopy, Electron, Scanning TransmissionTomography, Spiral ComputedMicroscopyTomography, OpticalHistocytochemistryMultidetector Computed TomographyAutoradiographyTomography, Emission-Computed, Single-PhotonOrganoidsStaining and LabelingHistological TechniquesElectron Probe MicroanalysisCone-Beam Computed TomographyImaging, Three-DimensionalCell BiologyImage Processing, Computer-AssistedOsmiumTomography, X-RayNucleocapsidMicroscopy, Atomic ForceFlagellaSoftwareMolecular Sequence DataIllusionsAnalytic Sample Preparation MethodsHeavy IonsHeavy Ion RadiotherapyLinear Energy TransferSpecimen HandlingAutomation, LaboratoryArchivesSuccinic AnhydridesBiological Science DisciplinesPeriodicals as TopicPubMedDirectories as TopicCongresses as TopicFellowships and ScholarshipsScienceCaliforniaArizonaHistory, 20th CenturyAttentional Blink
Focused ionDetectorSpectroscopyTemperaturesParticleReconstructionCrystallographyTransmission electronSpecimensCharacterizationSeries of 2D projection imagesConfocal microscope2019Structural biologyJEOLCorrelativeTomographicSoft X-ray micrStructuresVisible light microscopyVisualizationCryomicroscopyScanning probe microscopeDoseMolecularQuantitativeVirusesMagnificationErnst RuskaLight microscopesResearchersElementalSituAtomic force micrElectrical conductivitySubcellularCombinesImagesIncident electron beam2017Temperature
Focused ion12
Detector11
Spectroscopy6
Temperatures12
Particle14
Reconstruction6
Crystallography6
Transmission electron34
Specimens6
Characterization4
Series of 2D projection images2
Confocal microscope3
20192
  • Retrieved on September 21, 2019 from https://www.news-medical.net/life-sciences/Cryo-electron-Tomography-Applications.aspx. (news-medical.net)
  • Clabbers Max T. B., Gruene Tim, van Genderen Eric, Abrahams Jan Pieter (2019), Reducing dynamical electron scattering reveals hydrogen atoms, in Acta Crystallographica Section A Foundations and Advances , 75(1), 82-93. (snf.ch)
Structural biology3
JEOL5
  • It is complemented with two screening JEOL JEM-1400 120 kV microscopes and also includes a state of the art sample preparation laboratory and computational facilities. (epsoweb.org)
  • JEOL USA offers tomography support that consists of three software packages. (jeolusa.com)
  • World-renowned electron microscopists will join Dr. Xiaoqing Pan, Director of the University of California Irvine Materials Research Institute (IMRI), for the Grand Opening of the JEOL Center for Nanoscale Solutions and a three-day symposium June 6-8, 2018. (nanotech-now.com)
  • The JEOL Center for Nanoscale Solutions will be the most advanced electron microscopy cluster available for probing the atomic structure and properties of materials," says JEOL USA President Peter Genovese. (nanotech-now.com)
  • JEOL is pleased to introduce the JEM-ARM300F2 GRAND ARM™2 as the newest offering in the Atomic Resolution analytical Microscope (ARM) Series. (jeolusa.com)
Correlative2
Tomographic1
  • The Bionanoprobe is also the first imaging solution to combine ultra-high resolution trace element mapping with cryogenic sample preservation and tomographic capabilities. (xradia.com)
Soft X-ray micr3
Structures7
Visible light microscopy1
  • Previously, to examine cells and other samples at progressively higher resolutions, researchers typically switched between multiple techniques such as magnetic resonance imaging (MRI), computed tomography (CT), visible light microscopy and electron microscopy, often using different samples and different preparation techniques for each one. (xradia.com)
Visualization2
Cryomicroscopy2
Scanning probe microscope1
Dose3
  • Total dose for the entire +/- 60° tilt series was 34 electrons/Angstrom2. (jeolusa.com)
  • The Tecnai G2 TEM's are equipped with Gatan cryogenic TEM system with FEI low dose capability, the most advanced SDD EDS system, FEI's total solution for 3D imaging, FEI Eagle 4k high sensitivity CCD camera and the Windows XP operating system. (tulane.edu)
  • Electron tomography of single particles had been proposed by Hoppe and his co-workers , however, due to the radiation-sensitivity of biological macromolecules to electrons, it is not feasible to expose a biological molecule to the dose required to reveal the 3D structure from one hundred different projection images of the same molecule. (scirp.org)
Molecular4
  • The research team, led by Hong Zhou, UCLA professor of microbiology, immunology and molecular genetics, used cryo-electron microscopy to image the structure at 3.3 angstroms. (blogspot.com)
  • This allows samples produced with molecular beam epitaxy (MBE) or chemical vapor deposition (CVD) to be brought directly onto the microscope stage without exposing them to air. (witec.de)
  • Electron tomography sets the bar in cellular imaging resolution [ 1 , 2 ], providing highly detailed views of thin biological samples, while fluorescence imaging, in conjunction with endogenous fluorescent labels, reveals intricate molecular interactions within whole cells [ 3 , 4 ], but at a lower resolution. (emergtoplifesci.org)
  • At an intermediate submicrometric scale, ID21, the spectromicroscopy beamline, offers complementary tools to the NINA beamlines with an X-ray microscope in the tender X-ray domain (2-9 keV) for 2D X-ray fluorescence (XRF) and XAS, a FTIR microscope for molecular mapping, including a side branch that should soon provide users with a stable and easy to use instrument for 2D µXRF and µXRD mapping. (esrf.eu)
Quantitative5
Viruses2
Magnification1
Ernst Ruska1
  • The electron microscope (EM) instrument, initially developed by Ernst Ruska in the early nineteen thirties , became a routine scientific instrument during the nineteen fifties and sixties. (scirp.org)
Light microscopes1
  • Until the end of the 20th century, light microscopes were subject to a major restriction: their resolution was limited by the wave nature of light. (mpg.de)
Researchers6
Elemental3
  • Ivan Povstugar, Pyuck-Pa Choi, Steffen Neumeier, Alexander Bauer, Christopher H. Zenk, Mathias Göken, and Dierk Raabe, "Elemental partitioning and mechanical properties of Ti- and Ta-containing Co-Al-W-base superalloys studied by atom probe tomography and nanoindentation," Acta Materialia 78 , 78-85 (2014). (mpie.de)
  • WEMC provides technical support, training and consultation in the area of electron microscopy and elemental analysis. (wur.nl)
  • It is also possible to filter electrons based on their energy loss and thus create an elemental map or improve image quality by removing inelastically scattered electrons. (soquelec.com)
Situ2
  • The Core also features widefield fluorescence and color imaging microscopes that can perform integrated analysis and acquisition for high content imaging experiments and can image tissues in live animals in situ with macro to micro imaging resolution. (unc.edu)
  • Zeiss Sigma HD VP Field Emission SEM with EDAX EBSD and Octane Plus EDX, backscattered electron and variable pressure detectors, three axis micromanipulator, cryogenic-can and in-situ Ar ion sputter cleaning. (bristol.ac.uk)
Atomic force micr1
  • At a temperature of 5 Kelvin, one end of the DNA strand was then pulled upwards using the tip of an atomic force microscope. (bionity.com)
Electrical conductivity1
Subcellular1
Combines1
Images2
Incident electron beam1
  • The movement was induced by the incident electron beam at room temperature. (gatan.com)
20171
  • In 2017, the Nobel Prize in Chemistry was awarded to Jacques Dubochet , Joachim Frank , and Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution. (wikipedia.org)
Temperature1
  • The real vehicle environmental wind tunnel which can reproduce all environmental conditions, such as solar radiation which considered temperature (From cryogenic temperature to high temperature), humidity, and an illuminating angle. (calsonic.com)