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Cryoelectron MicroscopyFreezingCryoultramicrotomyMicroscopy, ElectronModels, MolecularAtadenovirusNegative StainingCapsidImage Processing, Computer-AssistedProtein ConformationModels, StructuralCapsid ProteinsElectron Microscope TomographyEncephalomyelitis, Western EquineVirionProtein Structure, QuaternaryCrystallography, X-RayTomographyVirus AssemblyMicroscopyRhinovirusCrystallizationProtein Structure, TertiaryImaging, Three-DimensionalX-Ray DiffractionCryopreservationImmunoglobulin Fab FragmentsBinding SitesMolecular ConformationMicroscopy, Electron, ScanningBiophysical PhenomenaMolecular Sequence DataProtein Structure, SecondaryAmino Acid SequenceBiophysicsMacromolecular SubstancesMicroscopy, ConfocalReceptors, VirusProtein SubunitsDimerizationProtein BindingMicroscopy, FluorescenceEndoplasmic Reticulum, RoughSequence Homology, Amino AcidEscherichia coliMicroscopy, Atomic ForceRecombinant ProteinsCell LineMicroscopy, Electron, TransmissionMicroscopy, ImmunoelectronSindbis Virus
Cryoelectron Microscopy
Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.
Freezing
Liquids transforming into solids by the removal of heat.
Cryoultramicrotomy
The technique of using a cryostat or freezing microtome, in which the temperature is regulated to -20 degrees Celsius, to cut ultrathin frozen sections for microscopic (usually, electron microscopic) examination.
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Models, Molecular
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Atadenovirus
A genus of ADENOVIRIDAE that comprises viruses of several species of MAMMALS and BIRDS. The type species is Ovine adenovirus D.
Negative Staining
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Capsid
The outer protein protective shell of a virus, which protects the viral nucleic acid.
Image Processing, Computer-Assisted
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Models, Structural
A representation, generally small in scale, to show the structure, construction, or appearance of something. (From Random House Unabridged Dictionary, 2d ed)
Capsid Proteins
Proteins that form the CAPSID of VIRUSES.
Electron Microscope Tomography
A tomographic technique for obtaining 3-dimensional images with transmission electron microscopy.
Encephalomyelitis, Western Equine
A form of arboviral encephalitis (which primarily affects horses) endemic to western and central regions of NORTH AMERICA. The causative organism (ENCEPHALOMYELITIS VIRUS, WESTERN EQUINE) may be transferred to humans via the bite of mosquitoes (CULEX tarsalis and others). Clinical manifestations include headache and influenza-like symptoms followed by alterations in mentation, SEIZURES, and COMA. DEATH occurs in a minority of cases. Survivors may recover fully or be left with residual neurologic dysfunction, including PARKINSONISM, POSTENCEPHALITIC. (From Joynt, Clinical Neurology, 1996, Ch26, pp8-9)
Virion
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
Protein Structure, Quaternary
The characteristic 3-dimensional shape and arrangement of multimeric proteins (aggregates of more than one polypeptide chain).
Crystallography, X-Ray
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Tomography
Imaging methods that result in sharp images of objects located on a chosen plane and blurred images located above or below the plane.
Virus Assembly
The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE.
Microscopy
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Rhinovirus
A genus of PICORNAVIRIDAE inhabiting primarily the respiratory tract of mammalian hosts. It includes over 100 human serotypes associated with the COMMON COLD.
Crystallization
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Imaging, Three-Dimensional
The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object.
X-Ray Diffraction
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Cryopreservation
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
Immunoglobulin Fab Fragments
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Binding Sites
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Molecular Conformation
The characteristic three-dimensional shape of a molecule.
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Biophysical Phenomena
The physical characteristics and processes of biological systems.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Protein Structure, Secondary
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Biophysics
The study of PHYSICAL PHENOMENA and PHYSICAL PROCESSES as applied to living things.
Macromolecular Substances
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
Microscopy, Confocal
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
Receptors, Virus
Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.
Protein Subunits
Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.
Dimerization
The process by which two molecules of the same chemical composition form a condensation product or polymer.
Protein Binding
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Microscopy, Fluorescence
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Endoplasmic Reticulum, Rough
A type of endoplasmic reticulum (ER) where polyribosomes are present on the cytoplasmic surfaces of the ER membranes. This form of ER is prominent in cells specialized for protein secretion and its principal function is to segregate proteins destined for export or intracellular utilization.
Sequence Homology, Amino Acid
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Microscopy, Atomic Force
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
Recombinant Proteins
Proteins prepared by recombinant DNA technology.
Cell Line
Established cell cultures that have the potential to propagate indefinitely.
Microscopy, Electron, Transmission
Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.
Microscopy, Immunoelectron
Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.
Sindbis Virus
The type species of ALPHAVIRUS normally transmitted to birds by CULEX mosquitoes in Egypt, South Africa, India, Malaya, the Philippines, and Australia. It may be associated with fever in humans. Serotypes (differing by less than 17% in nucleotide sequence) include Babanki, Kyzylagach, and Ockelbo viruses.

Cryo-electron microscopy structure of an SH3 amyloid fibril and model of the molecular packing. (1/1790)

Amyloid fibrils are assemblies of misfolded proteins and are associated with pathological conditions such as Alzheimer's disease and the spongiform encephalopathies. In the amyloid diseases, a diverse group of normally soluble proteins self-assemble to form insoluble fibrils. X-ray fibre diffraction studies have shown that the protofilament cores of fibrils formed from the various proteins all contain a cross-beta-scaffold, with beta-strands perpendicular and beta-sheets parallel to the fibre axis. We have determined the threedimensional structure of an amyloid fibril, formed by the SH3 domain of phosphatidylinositol-3'-kinase, using cryo-electron microscopy and image processing at 25 A resolution. The structure is a double helix of two protofilament pairs wound around a hollow core, with a helical crossover repeat of approximately 600 A and an axial subunit repeat of approximately 27 A. The native SH3 domain is too compact to fit into the fibril density, and must unfold to adopt a longer, thinner shape in the amyloid form. The 20x40-A protofilaments can only accommodate one pair of flat beta-sheets stacked against each other, with very little inter-strand twist. We propose a model for the polypeptide packing as a basis for understanding the structure of amyloid fibrils in general.  (+info)

MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member. (2/1790)

Terminal cell differentiation is correlated with the extensive sequestering of previously active genes into compact transcriptionally inert heterochromatin. In vertebrate blood cells, these changes can be traced to the accumulation of a developmentally regulated heterochromatin protein, MENT. Cryoelectron microscopy of chicken granulocyte chromatin, which is highly enriched with MENT, reveals exceptionally compact polynucleosomes, which maintain a level of higher order folding above that imposed by linker histones. The amino acid sequence of MENT reveals a close structural relationship with serpins, a large family of proteins known for their ability to undergo dramatic conformational transitions. Conservation of the "hinge region" consensus in MENT indicates that this ability is retained by the protein. MENT is distinguished from the other serpins by being a basic protein, containing several positively charged surface clusters, which are likely to be involved in ionic interactions with DNA. One of the positively charged domains bears a significant similarity to the chromatin binding region of nuclear lamina proteins and with the A.T-rich DNA-binding motif, which may account for the targeting of MENT to peripheral heterochromatin. MENT ectopically expressed in a mammalian cell line is transported into nuclei and is associated with intranuclear foci of condensed chromatin.  (+info)

Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules. (3/1790)

gamma-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1- 12). Here, we report the purification and characterization of both complexes that we name gamma-tubulin small complex (gammaTuSC; approximately 280,000 D) and Drosophila gammaTuRC ( approximately 2,200,000 D). In addition to gamma-tubulin, the gammaTuSC contains Dgrip84 and Dgrip91, two proteins homologous to the Spc97/98p protein family. The gammaTuSC is a structural subunit of the gammaTuRC, a larger complex containing about six additional polypeptides. Like the gammaTuRC isolated from Xenopus egg extracts (Zheng, Y., M.L. Wong, B. Alberts, and T. Mitchison. 1995. Nature. 378:578-583), the Drosophila gammaTuRC can nucleate microtubules in vitro and has an open ring structure with a diameter of 25 nm. Cryo-electron microscopy reveals a modular structure with approximately 13 radially arranged structural repeats. The gammaTuSC also nucleates microtubules, but much less efficiently than the gammaTuRC, suggesting that assembly into a larger complex enhances nucleating activity. Analysis of the nucleotide content of the gammaTuSC reveals that gamma-tubulin binds preferentially to GDP over GTP, rendering gamma-tubulin an unusual member of the tubulin superfamily.  (+info)

Cryoelectron microscopy of a nucleating model bile in vitreous ice: formation of primordial vesicles. (4/1790)

Because gallstones form so frequently in human bile, pathophysiologically relevant supersaturated model biles are commonly employed to study cholesterol crystal formation. We used cryo-transmission electron microscopy, complemented by polarizing light microscopy, to investigate early stages of cholesterol nucleation in model bile. In the system studied, the proposed microscopic sequence involves the evolution of small unilamellar to multilamellar vesicles to lamellar liquid crystals and finally to cholesterol crystals. Small aliquots of a concentrated (total lipid concentration = 29.2 g/dl) model bile containing 8.5% cholesterol, 22.9% egg yolk lecithin, and 68.6% taurocholate (all mole %) were vitrified at 2 min to 20 days after fourfold dilution to induce supersaturation. Mixed micelles together with a category of vesicles denoted primordial, small unilamellar vesicles of two distinct morphologies (sphere/ellipsoid and cylinder/arachoid), large unilamellar vesicles, multilamellar vesicles, and cholesterol monohydrate crystals were imaged. No evidence of aggregation/fusion of small unilamellar vesicles to form multilamellar vesicles was detected. Low numbers of multilamellar vesicles were present, some of which were sufficiently large to be identified as liquid crystals by polarizing light microscopy. Dimensions, surface areas, and volumes of spherical/ellipsoidal and cylindrical/arachoidal vesicles were quantified. Early stages in the separation of vesicles from micelles, referred to as primordial vesicles, were imaged 23-31 min after dilution. Observed structures such as enlarged micelles in primordial vesicle interiors, segments of bilayer, and faceted edges at primordial vesicle peripheries are probably early stages of small unilamellar vesicle assembly. A decrease in the mean surface area of spherical/ellipsoidal vesicles was correlated with the increased production of cholesterol crystals at 10-20 days after supersaturation by dilution, supporting the role of small unilamellar vesicles as key players in cholesterol nucleation and as cholesterol donors to crystals. This is the first visualization of an intermediate structure that has been temporally linked to the development of small unilamellar vesicles in the separation of vesicles from micelles in a model bile and suggests a time-resolved system for further investigation.  (+info)

Native display of complete foreign protein domains on the surface of hepatitis B virus capsids. (5/1790)

The nucleocapsid of hepatitis B virus (HBV), or HBcAg, is a highly symmetric structure formed by multiple dimers of a single core protein that contains potent T helper epitopes in its 183-aa sequence. Both factors make HBcAg an unusually strong immunogen and an attractive candidate as a carrier for foreign epitopes. The immunodominant c/e1 epitope on the capsid has been suggested as a superior location to convey high immunogenicity to a heterologous sequence. Because of its central position, however, any c/e1 insert disrupts the core protein's primary sequence; hence, only peptides, or rather small protein fragments seemed to be compatible with particle formation. According to recent structural data, the epitope is located at the tips of prominent surface spikes formed by the very stable dimer interfaces. We therefore reasoned that much larger inserts might be tolerated, provided the individual parts of a corresponding fusion protein could fold independently. Using the green fluorescent protein (GFP) as a model insert, we show that the chimeric protein efficiently forms fluorescent particles; hence, all of its structurally important parts must be properly folded. We also demonstrate that the GFP domains are surface-exposed and that the chimeric particles elicit a potent humoral response against native GFP. Hence, proteins of at least up to 238 aa can be natively displayed on the surface of HBV core particles. Such chimeras may not only be useful as vaccines but may also open the way for high resolution structural analyses of nonassembling proteins by electron microscopy.  (+info)

Flexibility of the major antigenic loop of foot-and-mouth disease virus bound to a Fab fragment of a neutralising antibody: structure and neutralisation. (6/1790)

The interaction of foot-and-mouth disease virus (FMDV) serotype C (clone C-S8c1) with a strongly neutralising monoclonal antibody (MAb) 4C4 has been studied by combining data from cryoelectron microscopy and x-ray crystallography. The MAb 4C4 binds to the exposed flexible GH-loop of viral protein 1 (VP1), which appears to retain its flexibility, allowing movement of the bound Fab. This is in striking contrast to MAb SD6, which binds to the same GH-loop of VP1 but exhibits no movement of the bound Fab when observed under identical conditions. However, MAbs 4C4 and SD6 have very similar neutralisation characteristics. The known atomic structure of FMDV C-S8c1 and that of the 4C4 Fab cocrystallised with a synthetic peptide corresponding to the GH-loop of VP1 were fitted to the cryoelectron microscope density map. The best fit of the 4C4 Fab is compatible only with monovalent binding of the MAb in agreement with the neutralisation data on 4C4 MAbs, Fab2s, and Fabs. The position of the bound GH-loop is related to other known positions of this loop by a hinge rotation about the base of the loop. The 4C4 Fab appears to interact almost exclusively with the G-H loop of VP1, making no other contacts with the viral capsid.  (+info)

Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type 1 virions. (7/1790)

Herpes simplex virus type 1 virions were examined by electron cryomicroscopy, allowing the three-dimensional structure of the infectious particle to be visualized for the first time. The capsid shell is identical to that of B-capsids purified from the host cell nucleus, with the exception of the penton channel, which is closed. The double-stranded DNA genome is organized as regularly spaced ( approximately 26 A) concentric layers inside the capsid. This pattern suggests a spool model for DNA packaging, similar to that for some bacteriophages. The bulk of the tegument is not icosahedrally ordered. However, a small portion appears as filamentous structures around the pentons, interacting extensively with the capsid. Their locations and interactions suggest possible roles for the tegument proteins in regulating DNA transport through the penton channel and binding to cellular transport proteins during viral infection.  (+info)

Effect of buffer conditions on the position of tRNA on the 70 S ribosome as visualized by cryoelectron microscopy. (8/1790)

The effect of buffer conditions on the binding position of tRNA on the Escherichia coli 70 S ribosome have been studied by means of three-dimensional (3D) cryoelectron microscopy. Either deacylated tRNAfMet or fMet-tRNAfMet were bound to the 70 S ribosomes, which were programmed with a 46-nucleotide mRNA having AUG codon in the middle, under two different buffer conditions (conventional buffer: containing Tris and higher Mg2+ concentration [10-15 mM]; and polyamine buffer: containing Hepes, lower Mg2+ concentration [6 mM], and polyamines). Difference maps, obtained by subtracting 3D maps of naked control ribosome in the corresponding buffer from the 3D maps of tRNA.ribosome complexes, reveal the distinct locations of tRNA on the ribosome. The position of deacylated tRNAfMet depends on the buffer condition used, whereas that of fMet-tRNAfMet remains the same in both buffer conditions. The acylated tRNA binds in the classical P site, whereas deacylated tRNA binds mostly in an intermediate P/E position under the conventional buffer condition and mostly in the position corresponding to the classical P site, i. e. in the P/P state, under the polyamine buffer conditions.  (+info)

ASTM E3143 - 18b Standard Practice for Performing Cryo-Transmission Electron Microscopy of LiposomesASTM E3143 - 18b Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes
E3143-18b Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes vitrification~ transmission electron microscopes~
https://www.astm.org/Standards/E3143.htm
Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies<...Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies<...
TY - JOUR. T1 - Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies. AU - Yu, Guimei. AU - Vago, Frank. AU - Zhang, Dongsheng. AU - Snyder, Jonathan E.. AU - Yan, Rui. AU - Zhang, Ci. AU - Benjamin, Christopher. AU - Jiang, Xi. AU - Kuhn, Richard J.. AU - Serwer, Philip. AU - Thompson, David H.. AU - Jiang, Wen. N1 - Funding Information: This work was supported in part by NIH Grant R01AI072035 . The negative staining TEM and cryo-EM images were taken at the Purdue Biological Electron Microscopy Facility. We thank Melissa Mikolaj and Thomas J. Edwards for providing some of the Sindbis virus test samples. PY - 2014/7. Y1 - 2014/7. N2 - Single particle cryo-electron microscopy (cryo-EM) is an emerging powerful tool for structural studies of macromolecular assemblies (i.e., protein complexes and viruses). Although single particle cryo-EM requires less concentrated and smaller amounts of samples than X-ray crystallography, it ...
https://scholars.uthscsa.edu/en/publications/single-step-antibody-based-affinity-cryo-electron-microscopy-for-
ASSISTANT PROFESSOR IN CRYO ELECTRON MICROSCOPY job with University of California, Irvine | 417876ASSISTANT PROFESSOR IN CRYO ELECTRON MICROSCOPY job with University of California, Irvine | 417876
ASSISTANT PROFESSOR IN CRYO ELECTRON MICROSCOPY in Full Time, Academia, Life Sciences with University of California, Irvine. Apply Today.
https://jobs.sciencecareers.org/job/417876/assistant-professor-in-cryo-electron-microscopy/
Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs | SpringerLinkSingle-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs | SpringerLink
Single-particle cryo-electron microscopy has become an indispensable technique in structural biology. In particular when studying membrane proteins, it allows the use of membrane-mimicking tools,...
https://link.springer.com/protocol/10.1007%2F978-1-0716-0373-4_17
Improved methods for single-particle cryogenic electron microscopyImproved methods for single-particle cryogenic electron microscopy
Author(s): Palovcak, Eugene Joseph | Advisor(s): Cheng, Yifan | Abstract: Biological macromolecules such as enzymes are nanoscale machines. This is true in a concrete sense: if the atomic structure of a biological macromolecule can be obtained, the theories of mechanics and intermolecular forces can be applied to explain how the machine works in terms that engineers would understand, including motors, ratchets, gates and transducers. Nevertheless, biological macromolecules are complex, fragile and extremely small, so obtaining their structures is a challenging experimental endeavor. Single-particle cryogenic electron microscopy (cryo-EM) is a technique for determining the 3D structure of a biological macromolecule from a large set of 2D electron micrographs of individual structurally-identical particles. To obtain such images, a solution of the macromolecules must be prepared in the frozen-hydrated state, embedded in a thin electron-transparent glassy film of water. This specimen must then be imaged
https://escholarship.org/uc/item/51r7k7hw
Crucial steps in the structure determination of a coronavirus spike glycoprotein using cryo-electron microscopy | Research -...Crucial steps in the structure determination of a coronavirus spike glycoprotein using cryo-electron microscopy | Research -...
Link to Pubmed [PMID] - 27667334. Protein Sci. 2017 01;26(1):113-121. The tremendous pandemic potential of coronaviruses was demonstrated twice in the last 15 years by two global outbreaks of deadly pneumonia. Entry of coronaviruses into cells is mediated by the transmembrane spike glycoprotein S, which forms a trimer carrying receptor-binding and membrane fusion functions. Despite their biomedical importance, coronavirus S glycoproteins have proven difficult targets for structural characterization, precluding high-resolution studies of the biologically relevant trimer. Recent technological developments in single particle cryo-electron microscopy allowed us to determine the first structure of a coronavirus S glycoprotein trimer which provided a framework to understand the mechanisms of viral entry and suggested potential inhibition strategies for this family of viruses. Here, we describe the key factors that enabled this breakthrough.. https://www.ncbi.nlm.nih.gov/pubmed/27667334 ...
https://research.pasteur.fr/en/publication/crucial-steps-in-the-structure-determination-of-a-coronavirus-spike-glycoprotein-using-cryo-electron-microscopy/
Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopyStructural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy
Abstract: We used cryogenic transmission electron microscopy (cryo-TEM) to identify differences in macromolecular structures present in the serum from healthy individuals (HI) and prostate cancer (PCa) patients and show that these differences are potential markers for disease. Using a murine orthotopic model of human PCa, we determined that some of these structural markers in serum are associated with the tumour burden. These findings signify the potential of this nanoscale ex vivo imaging technology of body fluids as a platform for discovering early markers of PCa and other diseases.. ...
https://scialert.net/asci/ascidetail.php?doi=jmicro.2010.451.456&kw=
Resolving the Morphology of Peptoid Vesicles at the 1 nm Length Scale Using Cryogenic Electron Microscopy.Resolving the Morphology of Peptoid Vesicles at the 1 nm Length Scale Using Cryogenic Electron Microscopy.
Author(s): Jiang, Xi; Spencer, Ryan K; Sun, Jing; Ophus, Colin; Zuckermann, Ronald N; Downing, Kenneth H; Balsara, Nitash P | Abstract: Vesicle formation in a series of amphiphilic sequence-defined polypeptoid block co-polymers comprising a phosphonated hydrophilic block and an amorphous hydrophobic block, poly- N-(2-ethyl)hexylglycine- block-poly- N-phosphonomethylglycine (pNeh- b-pNpm), is studied. The hydrophobic/hydrophilic block ratio was varied keeping the total chain length of the co-polymers constant. A new approach for characterizing the vesicle membrane morphology based on low-dose cryogenic electron microscopy (cryo-EM) is described. The individual low-dose micrographs cannot be interpreted directly due to low signal-to-noise ratio. Sorting and averaging techniques, developed in the context of protein structure determination, were thus applied to vesicle micrographs. Molecular dynamic simulations of the vesicles were used to establish the relationship between membrane morphology and averaged
https://escholarship.org/uc/item/7n15h4b6
Structural studies of virus-antibody complexes by electron cryomicroscopy and X-ray crystallography<...Structural studies of virus-antibody complexes by electron cryomicroscopy and X-ray crystallography<...
TY - JOUR. T1 - Structural studies of virus-antibody complexes by electron cryomicroscopy and X-ray crystallography. AU - Chiu, Wah. AU - Smith, Thomas. PY - 1994. Y1 - 1994. N2 - The combined use of electron cryomicroscopy and X-ray crystallography has recently provided unprecedented and unique structural information of virus-antibody complexes. Different kinds of viral proteins have been located and identified on the capsid surface, certain residues of the viral proteins involved in antibody interactions have been identified; the elbow angle of bound antibody has been measured; and the mechanism of antibody-mediated neutralization has been elucidated. Within the next few years, this combined methodology should help investigators resolve the structures of large macromolecular assemblies to even higher resolutions.. AB - The combined use of electron cryomicroscopy and X-ray crystallography has recently provided unprecedented and unique structural information of virus-antibody complexes. ...
https://researchexperts.utmb.edu/en/publications/structural-studies-of-virus-antibody-complexes-by-electron-cryomi
RCSB PDB - 3JBP: Cryo-electron microscopy reconstruction of the Plasmodium falciparum 80S ribosome bound to E-tRNARCSB PDB - 3JBP: Cryo-electron microscopy reconstruction of the Plasmodium falciparum 80S ribosome bound to E-tRNA
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
https://www.rcsb.org/structure/3JBP
Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics. - Nuffield Department of Medicine
Retroviral capsid proteins are conserved structurally but assemble into different morphologies. The mature human immunodeficiency virus-1 (HIV-1) capsid is best described by a fullerene cone model, in which hexamers of the capsid protein are linked to form a hexagonal surface lattice that is closed by incorporating 12 capsid-protein pentamers. HIV-1 capsid protein contains an amino-terminal domain (NTD) comprising seven α-helices and a β-hairpin, a carboxy-terminal domain (CTD) comprising four α-helices, and a flexible linker with a 310-helix connecting the two structural domains. Structures of the capsid-protein assembly units have been determined by X-ray crystallography; however, structural information regarding the assembled capsid and the contacts between the assembly units is incomplete. Here we report the cryo-electron microscopy structure of a tubular HIV-1 capsid-protein assembly at 8 Å resolution and the three-dimensional structure of a native HIV-1 core by cryo-electron tomography. The
https://www.ndm.ox.ac.uk/publications/665213
The resolution revolution continues: GIF Quantum LS imaging filter with K2 Summit camera enables scientists to reach 2.2 Å...The resolution revolution continues: GIF Quantum LS imaging filter with K2 Summit camera enables scientists to reach 2.2 Å...
Structural biology is undergoing a seismic shift as single-particle cryo-electron microscopy (cryo-EM) is increasingly becoming the technique of choice for scientists who want to study DNA, proteins and other biological molecules at atomic resolution. Having just recently broken the 3 Å (angstrom) barrier with the use of the Gatan K2 Summit® direct detection camera, a new structure shows that there is no longer a resolution gap between x-ray crystallography and cryo-EM.
http://www.gatan.com/company/news/resolution-revolution-continues-gif-quantum-ls-imaging-filter-k2-summit-camera-enables
Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome.
Cullin-Ring E3 Ligases (CRLs) regulate a multitude of cellular pathways through specific substrate receptors. The COP9 signalosome (CSN) deactivates CRLs by removing NEDD8 from activated Cullins. Here we present structures of the neddylated and deneddylated CSN-CRL2 complexes by combining single-particle cryo-electron microscopy (cryo-EM) with chemical cross-linking mass spectrometry (XL-MS). These structures suggest a conserved mechanism of CSN activation, consisting of conformational clamping of the CRL2 substrate by CSN2/CSN4, release of the catalytic CSN5/CSN6 heterodimer and finally activation of the CSN5 deneddylation machinery. Using hydrogen-deuterium exchange (HDX)-MS we show that CRL2 activates CSN5/CSN6 in a neddylation-independent manner. The presence of NEDD8 is required to activate the CSN5 active site. Overall, by synergising cryo-EM with MS, we identify sensory regions of the CSN that mediate its stepwise activation and provide a framework for understanding the regulatory ...
https://repository.icr.ac.uk/handle/internal/3354
Using molecular approaches to understand Complex I deficiency in mouse models
Complex I (NADH:ubiquinone oxidoreductase), a major electron entry point to the mitochondrial respiratory chain, couples electron transfer from NADH to ubiquinone to proton pumping across the mitochondrial inner membrane, and generates the proton motive force that drives ATP synthesis and transport processes. The ~1 MDa mammalian complex contains 45 subunits, and pathological mutations in both its mitochondrial and nuclear encoded subunits result in diverse neuro-muscular disorders. Recent high-resolution mammalian complex I structures have been solved by single-particle cryo-electron microscopy (cryo-EM) in characterised biological states and provide mechanistic insights. However, the molecular bases of genetically-determined complex I dysfunctions remain unclear. Here, two mouse models of complex I-linked mitochondrial disease were analysed structurally by cryo-EM to understand the mechanisms of their pathogenesis. The first part of this thesis explores complex I from the ND6-P25 mouse model, ...
https://www.repository.cam.ac.uk/handle/1810/323944
High resolution single-particle cryo-EM of a 200 kDa membrane ion channel | NIH Research FestivalHigh resolution single-particle cryo-EM of a 200 kDa membrane ion channel | NIH Research Festival
Magnesium ions are the most abundant divalent intracellular cations and are essential for life, as they play key roles in signaling, nucleic acid action and metabolism. Mg2+-deficiency is associated with diseases affecting the heart, muscle, bone, immune and nervous system, so it is very important to fully understand this ion uptake system. The ~200 kDa pentameric membrane channel CorA is the major Mg2+ uptake system in bacteria and a homolog of the eukaryotic mitochondrial Mrs2 proteins which it can complement. CorA contributes to Mg2+ homeostasis through a negative feedback loop, where Mg2+ binding at the subunit interface leads to channel closure and low Mg2+ concentrations stabilize the open conformation. Electron paramagnetic resonance (EPR) spectroscopic studies of purified CorA revealed large quaternary conformational changes associated with magnesium binding/unbinding. Using single-particle cryo-EM, we have determined the structure for the closed magnesium-bound state of CorA at a ...
https://researchfestival.nih.gov/2015/posters/high-resolution-single-particle-cryo-em-200-kda-membrane-ion-channel
Cryo Electron Microscopy Short Course 2010 | IPRIMECryo Electron Microscopy Short Course 2010 | IPRIME
Alon McCormick, course coordinator and IPrime NMP Program Leader. This course is intended to enable industrial and academic scientists and engineers with no prior knowledge of electron microscopy to gain insight into cryotechniques to image nanostructures in liquids, soft materials, and biological systems. After attending the course you will be better able to assess what experiments are feasible for samples of interest to you, and to estimate the benefits and costs of these approaches.. The main instructors are innovators and leaders in the field of cryo-electron microscopy, particularly in the study of soft materials, nanomaterials, and virus structure - Jayesh Bellare, Indian Institute of Technology, Bombay, Ishi Talmon, Technion, Israel Institute of Technology, Wei Zhang, U of MN CharFac & Institute for Molecular Virology.. Registration and fees ...
http://www.iprime.umn.edu/cryo-electron-microscopy-short-course-2010
Solubilization of nonionic monoalkyl amphiphile-cholesterol vesicles by octyl glucoside: Cryo-transmission electron microscopy...Solubilization of nonionic monoalkyl amphiphile-cholesterol vesicles by octyl glucoside: Cryo-transmission electron microscopy...
Addresses: UNIV PARIS SUD, EQUIPE PHYSICOCHIM SYST POLYPHASES, URA CNRS 1218, CHATENAY MALABRY, FRANCE. UNIV UPPSALA, INST PHYS CHEM, S-75121 UPPSALA, SWEDEN.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-03-21 ...
http://uu.diva-portal.org/smash/record.jsf?pid=diva2:103010
High resolution cryo-electron microscopy of clathrin cage networks at University of Warwick on FindAPhD.comHigh resolution cryo-electron microscopy of clathrin cage networks at University of Warwick on FindAPhD.com
PhD Project - High resolution cryo-electron microscopy of clathrin cage networks at University of Warwick, listed on FindAPhD.com
https://www.findaphd.com/search/projectdetails.aspx?PJID=79617&LID=1573
Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric G<sub>i</sub> ...
TY - JOUR. T1 - Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric Gi AU - Qi, Xiaofeng. AU - Liu, Heng. AU - Thompson, Bonne. AU - McDonald, Jeffrey. AU - Zhang, Cheng. AU - Li, Xiaochun. N1 - Publisher Copyright: © 2019, The Author(s), under exclusive licence to Springer Nature Limited.. PY - 2019/7/11. Y1 - 2019/7/11. N2 - The oncoprotein Smoothened (SMO), a G-protein-coupled receptor (GPCR) of the Frizzled-class (class-F), transduces the Hedgehog signal from the tumour suppressor Patched-1 (PTCH1) to the glioma-associated-oncogene (GLI) transcription factors, which activates the Hedgehog signalling pathway1,2. It has remained unknown how PTCH1 modulates SMO, how SMO is stimulated to form a complex with heterotrimeric G proteins and whether G-protein coupling contributes to the activation of GLI proteins3. Here we show that 24,25-epoxycholesterol, which we identify as an endogenous ligand of PTCH1, can stimulate Hedgehog signalling in cells and can trigger ...
https://utsouthwestern.pure.elsevier.com/en/publications/cryo-em-structure-of-oxysterol-bound-human-smoothened-coupled-to-
Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome<...
TY - JOUR. T1 - Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome. AU - Armache, Jean Paul. AU - Jarasch, Alexander. AU - Anger, Andreas M.. AU - Villa, Elizabeth. AU - Becker, Thomas. AU - Bhushan, Shashi. AU - Jossinet, Fabrice. AU - Habeck, Michael. AU - Dindar, Gülcin. AU - Franckenberg, Sibylle. AU - Marquez, Viter. AU - Mielke, Thorsten. AU - Thomm, Michael. AU - Berninghausen, Otto. AU - Beatrix, Birgitta. AU - Söding, Johannes. AU - Westhof, Eric. AU - Wilson, Daniel N.. AU - Beckmann, Roland. PY - 2010/11/16. Y1 - 2010/11/16. N2 - Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome ...
https://pennstate.pure.elsevier.com/en/publications/localization-of-eukaryote-specific-ribosomal-proteins-in-a-55-%C3%A5-c
ccp4bb] EMBO practical course on image processing for cryo-electron microscopy
Posted on behalf of Elena Orlova... Dear All, It is a pleasure to announce that we are running the next EMBO practical course on image processing for cryo-electron microscopy in Birkbeck College , ISMB, London . This 10 day course will be held in September (5 - 15), 2017. You can find our announcement on the following sites: Twitter ( https://twitter.com/EMBOevents/status/818487485814730759 ), Facebook ( https://www.facebook.com/events/590421591166869/ ) EMBO events calendar ( http://www.embo.org/events/events-calendar ). Birkbeck College (http://www.bbk.ac.uk/biology/about-us/events/image-processing-for-cryo-electron-microscopy-1) Deadline for applications: Monday 15 May 2017 The aim of the course is to teach the basic principles and practical aspects of image processing to bioscientists and structural biologists wishing to determine macromolecular structures by cryo electron microscopy (EM). The course will concentrate on processing of single particle images, and will be aimed at advanced PhD ...
http://www.ccp4.ac.uk/news/msg00021.html
single-particle electron cryomicroscopy Archives | The Emory Wheelsingle-particle electron cryomicroscopy Archives | The Emory Wheel
The Robert P. Apkarian Integrated Electron Microscopy Core (IECM) facility at Cherry Logan Emerson Hall is expanding to the O. Wayne Rollins Research Center, according to Assistant Dean of Research at the Emory University School of Medicine Michael E. Zwick. ... ...
http://emorywheel.com/tag/single-particle-electron-cryomicroscopy/
Cryo electron microscopy of membrane proteins and biomolecular assemblies - Hans Hebert | Department of Biosciences and...Cryo electron microscopy of membrane proteins and biomolecular assemblies - Hans Hebert | Department of Biosciences and...
Mucins are large glycoproteins that coat the surface of cells in the respiratory, digestive, and urogenital tracts. The first insights into the packing and secretion of intestinal MUC2 in molecular terms have now been obtained. Purified MUC2-N protein was analyzed by electron microscopy and image processing, suggesting how MUC2-N is packed in granulae of goblet cells. Dysregulation of the normal |1000-fold expansion upon release could explain the extreme viscosity of the produced mucus seen in cystic fibrosis.
http://ki.se/en/bionut/cryo-electron-microscopy-of-membrane-proteins-and-biomolecular-assemblies-hans-hebert
CryoEM Structure Refinement by Integrating NMR Chemical Shifts with Molecular Dynamics Simulations. - Nuffield Department of...
Single particle cryoEM has emerged as a powerful method for structure determination of proteins and complexes, complementing X-ray crystallography and NMR spectroscopy. Yet, for many systems, the resolution of cryoEM density map has been limited to 4-6 Å, which only allows for resolving bulky amino acids side chains, thus hindering accurate model building from the density map. On the other hand, experimental chemical shifts (CS) from solution and solid state MAS NMR spectra provide atomic level data for each amino acid within a molecule or a complex; however, structure determination of large complexes and assemblies based on NMR data alone remains challenging. Here, we present a novel integrated strategy to combine the highly complementary experimental data from cryoEM and NMR computationally by molecular dynamics simulations to derive an atomistic model, which is not attainable by either approach alone. We use the HIV-1 capsid protein (CA) C-terminal domain as well as the large capsid assembly to
https://www.ndm.ox.ac.uk/publications/681925
Single particle analysis - WikipediaSingle particle analysis - Wikipedia
Single particle analysis is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM). These methods were developed to improve and extend the information obtainable from TEM images of particulate samples, typically proteins or other large biological entities such as viruses. Individual images of stained or unstained particles are very noisy, and so hard to interpret. Combining several digitized images of similar particles together gives an image with stronger and more easily interpretable features. An extension of this technique uses single particle methods to build up a three-dimensional reconstruction of the particle. Using cryo-electron microscopy it has become possible to generate reconstructions with sub-nanometer resolution and near-atomic resolution first in the case of highly symmetric viruses, and now in smaller, asymmetric proteins as well. Single particle analysis can be done on both negatively stained and vitreous ...
https://en.wikipedia.org/wiki/Single_particle_analysis
Single-particle cryo-EM of the ryanodine receptor from skeletal muscle by Alex H. WillSingle-particle cryo-EM of the ryanodine receptor from skeletal muscle by Alex H. Will
The RyR1 acts as an intracellular calcium channel that allows passage of Ca2+ from the sarcoplasmic reticulum to the cytoplasm; this increase in cytosolic Ca2+ is required for excitation-contraction coupling. Certain mutations in RyR1 have been directly linked to malignant hyperthermia (MH) and central core disease. In a series of studies, the role of Mg2+ has been explored as it pertains to MH, and it has been determined that dysregulation of Mg2+ can even lead to MH in patients without mutations. Consequently, the aim of the study was to insert the RyR1 into nanodiscs, small, circular lipid bilayers used to solubilize membrane proteins, and to use cryo-electron microscopy to assess the conformation of the RyR1 in the presence of Mg2+ and AMP-PCP. Particle reconstruction generated a 9.0 Å resolution map that confirmed successful incorporation of the RyR1 into nanodiscs and allowed visualization of the RyR1 in a physiological closed state.
https://scholarscompass.vcu.edu/etd/4998/
A Bayesian adaptive basis algorithm for single particle reconstruction. - PubMed - NCBIA Bayesian adaptive basis algorithm for single particle reconstruction. - PubMed - NCBI
J Struct Biol. 2012 Jul;179(1):56-67. doi: 10.1016/j.jsb.2012.04.012. Epub 2012 May 1. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
https://www.ncbi.nlm.nih.gov/pubmed?term=22564910
Events - Instruct-ERICEvents - Instruct-ERIC
Agenda. Webinar moderator: Patrick Schultz, IGBMC Strasbourg. Introduction - Integrated Structural Biology at the Centre for Integrative Biology / IGBMC. Speaker: Bruno Klaholz. Talk 1: Structural basis for the allosteric regulation of Human Topoisomerase 2α. Speaker: Valérie Lamour. Affiliation: IGBMC Strasbourg. Abstract: The human type IIA topoisomerases (Top2) are essential enzymes that regulate DNA topology and chromosome organization. The Top2α isoform is a prime target for antineoplastic compounds used in cancer therapy that form ternary cleavage complexes with the DNA. Despite extensive studies, structural information on this large dimeric assembly is limited to the catalytic domains, hindering the exploration of allosteric mechanism governing the enzyme activities. We produced the entire human Top2α in mammalian cells and present cryo-EM structures of the entire human Top2α nucleoprotein complex in different conformations at subnanometer resolutions. Our data unveils the molecular ...
https://instruct-eric.eu/events/instruct-eric-webinar-series-structure-meets-function---webinar-10/
Stringent Response: Tet(O) cryoEM structure is out!Stringent Response: Tet(O) cryoEM structure is out!
So it is finally out: the cryoEM structure of Tet(O) on the ribosome we have collaborated on with Joachim Franks lab is finally published on in Nature Communications. Tet(O) is a bacterial translational GTPase that clears the ribosome from tetracycline antibiotic, and structural data provided in the paper shed light on the mechanism of Tet(O)-mediated resistance. Recently cryoEM of Tet(O)s close relative, Tet(M) was published by Beckmann and Wilson labs, so now one can compare the two. And yes, they look very similar. No surprise there. ...
http://stringentresponse.blogspot.com/2013/02/teto-cryoem-structure-is-out.html
PDB-6id0: Cryo-EM structure of a human intron lariat spliceosome prior to P... - YorodumiPDB-6id0: Cryo-EM structure of a human intron lariat spliceosome prior to P... - Yorodumi
Pre-mRNA splicing is executed by the spliceosome, which has eight major functional states each with distinct composition. Five of these eight human spliceosomal complexes, all preceding exon ligation, have been structurally characterized. In this study, we report the cryo-electron microscopy structures of the human post-catalytic spliceosome (P complex) and intron lariat spliceosome (ILS) at average resolutions of 3.0 and 2.9 Å, respectively. In the P complex, the ligated exon remains anchored to loop I of U5 small nuclear RNA, and the 3-splice site is recognized by the junction between the 5-splice site and the branch point sequence. The ATPase/helicase Prp22, along with the ligated exon and eight other proteins, are dissociated in the P-to-ILS transition. Intriguingly, the ILS complex exists in two distinct conformations, one with the ATPase/helicase Prp43 and one without. Comparison of these three late-stage human spliceosomes reveals mechanistic insights into exon release and spliceosome ...
https://pdbj.org/emnavi/quick.php?id=pdb-6id0
PDB-5n5n: Cryo-EM structure of tsA201 cell alpha1B and betaI and betaIVb mi... - YorodumiPDB-5n5n: Cryo-EM structure of tsA201 cell alpha1B and betaI and betaIVb mi... - Yorodumi
Joachim Frank (Columbia University, New York, USA) is a pioneer of single particle reconstruction, which is the most used reconstruction method for 3DEM structures in EMDB and EM entries in PDB. And also, he is a develper of Spider, which is one of the most famous software in this field, and is used for some EM Navigor data (e.g. map projection/slice images ...
https://pdbj.org/emnavi/quick.php?id=pdb-5n5n
News : EMDataResourceNews : EMDataResource
The use of 3D cryo-electron microscopy reconstruction (3DEM) methods to experimentally determine structures of biological macromolecules, complexes and machines is rapidly increasing in the structural biology community. For journal articles reporting new 3DEM structures via single particle, helical, electron crystallography, and sub-tomogram averaging methods, we find that the overall map-deposition rate to EM Data Bank (EMDB) is approaching 70%. However, the rate varies significantly per journal, and tends to be highest for journals with well-defined and consistently enforced 3DEM deposition policies in place ...
https://www.emdataresource.org/allnews.html
How smart, ultrathin nanosheets go fishing for proteins | Max Planck Institute of BiophysicsHow smart, ultrathin nanosheets go fishing for proteins | Max Planck Institute of Biophysics
With our process, new types of proteins can be isolated from mixtures and characterized within a week, explains Daniel Rhinow from the Max Planck Institute of Biophysics. To date, just the isolation of the proteins was often part of a doctorate lasting several years. Together with Andreas Terfort (Goethe University Frankfurt) and Andrey Turchanin (Friedrich Schiller University Jena), the idea evolved a few years ago of fishing the desired proteins directly out of mixtures by equipping a nanosheet with recognition sites onto which the target protein bonds. The researchers have now succeeded in making proteins directly available for examination using electron cryo-microscopy through a smart nanosheet.. Cryo electron microscopy is based on the shock- reezing of a sample at temperatures below minus 150 degrees Celsius. During this process, the protein maintains its structure, no interfering fixing and coloring agents are needed, and the electrons can easily irradiate the frozen object. The ...
https://www.biophys.mpg.de/2299068/news_publication_15177567_transferred?c=2360
Spectroscopy Society of Pittsburgh » February Monthly MeetingSpectroscopy Society of Pittsburgh » February Monthly Meeting
at the local three-fold axis in the cryoEM map was confirmed by mutagenesis to be essential for function. More recently, the cryo-EM structure of the tube was solved at 8Å resolution and this cryo-EM structure allowed unambiguous modeling and refinement by large-scale molecular dynamics (MD) simulation, resulting in all-atom models for the hexamer-of-hexamer and pentamer-of-hexamer elements of spheroidal capsids. Furthermore, the 3D structure of a native HIV-1 core was determined by cryo-electron tomography (Cryo-ET), which in combination with MD simulations permitted the construction of a realistic all-atom model for the entire capsid, based on the 3D authentic core structure. ...
https://www.ssp-pgh.org/february-2014-monthly-meeting/
Forschungszentrum Jülich - Ansprechpartner - Prof. Dr. Carsten SachseForschungszentrum Jülich - Ansprechpartner - Prof. Dr. Carsten Sachse
I am the director of the newly founded Ernst-Ruska-Centre 3 for Structural Biology at the Forschungszentrum Jülich. I was a group leader at the EMBL from 2010 - 2018. I got in touch with electron cryomicroscopy by studying amyloid fibrils in the groups of Marcus Fändrich (now in Ulm, Germany) and Niko Grigorieff (now at Janelia Farm, USA).. For my postdoctoral period, I went to the MRC Laboratory of Molecular Biology (Cambridge, UK) to work with Richard Henderson and Roger Williams. In the last few years, we set up a group to study the mechanisms of autophagy by electron cryomicroscopy. Traditionally, I have been interested in the advancement of high-resolution single-particle EM with a particular focus on helical reconstruction. These developments have led to our recent helical processing software package Spring. More recently, we became interested in improving means of cryo-EM map interpretation by atomic models.. ...
https://kfa-juelich.de/SharedDocs/Personen/ER-C/ER-C-3/EN/Sachse_C.html?nn=2090670
Electron cryomicroscopy shows cardiac thin filament structure -- MEDICA - World Forum for MedicineElectron cryomicroscopy shows cardiac thin filament structure -- MEDICA - World Forum for Medicine
Researchers at Osaka University used electron cryomicroscopy (CryoEM) to image essential cardiac muscle components, known as thin filaments, with unprecedented resolution. They also discovered the mechanism by which these filaments regulate heartbeat via cardiac muscle contractions in the presence or absence of calcium ions by changing their conformations.
https://www.medica-tradefair.com/en/News/Archive/Electron_cryomicroscopy_shows_cardiac_thin_filament_structure
APPROACHING VIRUS LIFE CYCLE WITH ELECTRON MICROSCOPY | IMRIAPPROACHING VIRUS LIFE CYCLE WITH ELECTRON MICROSCOPY | IMRI
US Air Force Research Lab. Monday, April 24, 2017, Colloquia Auditorium. Electron microscopy has been one of the robust tools in characterizing the life cycle of viruses, such as Semliki Forest Virus (SFV), an alphavirus that belongs to Togavirus family and causes encephalitis in human. The viral particle of SFV is composed of a nucleocapsid enclosed by a lipid-anchored glycoprotein spikes and infects cell through clathrin-dependent endocytosis pathway where it releases the nucleocapsid into the cytoplasm after membrane fusion at the late endosome. Cryo-electron microscopy and single particle reconstruction revealed the structure of SFV virion that both the nucleocapsid and surface spikes adapted into icosahedral symmetry with T4 surface lattice and that the connection between nucleocapsid protein and C-terminal tail of the glycoprotein stabilizes the particle integrity. Mutation at nucleocapsid does not changed the assembly of the viral particle although truncation at the RNA-binding domain ...
http://imri.uci.edu/node/291
Cryoelectron Microscopy of Icosahedral Virus Particles | SpringerLinkCryoelectron Microscopy of Icosahedral Virus Particles | SpringerLink
With the rapid progresses in both instrumentation and computing, it is increasingly straightforward and routine to determine the structures of icosahedral viruses to subnanometer resolutions (6-10 Å)...
https://link.springer.com/protocol/10.1007%2F978-1-59745-294-6_17
Zinc in the structure of Drosophila Nod (3DC4) and Bovine Tubulin (1JFF) Docked Into The 11-Angstrom Cryo-Em Map of Nucleotide...
Mono- and Stereopictres of 5.0 Angstrom coordination sphere of Zinc atom in PDB 3dco: Drosophila Nod (3DC4) and Bovine Tubulin (1JFF) Docked Into The 11-Angstrom Cryo-Em Map of Nucleotide-Free Nod Complexed to the Microtubule
http://zinc.atomistry.com/pdb3dco.html
Publications
by Victor A. Kostyuchenko, Elisa X. Y. Lim, Shuijun Zhang, Guntur Fibriansah, Thiam-Seng Ng, Justin S. G. Ooi, Jian Shi & Shee-Mei Lok. Nature (2016) doi:10.1038/nature17994 Published online 19 April 2016. Zika virus (ZIKV), formerly a neglected pathogen, has recently been associated with microcephaly in fetuses1, and with Guillian-Barré syndrome in adults2. Here we present the 3.7 Å resolution cryo-electron microscopy structure of ZIKV, and show that the overall architecture of the virus is similar to that of other flaviviruses. Sequence and structural comparisons of the ZIKV envelope (E) protein with other flaviviruses show that parts of the E protein closely resemble the neurovirulent West Nile and Japanese encephalitis viruses, while others are similar to dengue virus (DENV). However, the contribution of the E protein to flavivirus pathobiology is currently not understood. The virus particle was observed to be structurally stable even when incubated at 40 °C, in sharp contrast to the less ...
https://cbis.nus.edu.sg/category/publications/
New indication of a link between Alzheimers and diabetes | EurekAlert! Science NewsNew indication of a link between Alzheimers and diabetes | EurekAlert! Science News
Pathological protein clumps are characteristic of a series of diseases, such as Alzheimers disease, Parkinsons disease, and type 2 diabetes. Scientists at Forschungszentrum Jülich, Heinrich Heine University Düsseldorf, and Maastricht University have now used cryo-electron microscopy to obtain a sharp image for the first time of how individual molecules are arranged in protein strings, which constitute the deposits typical for diabetes. The structure of the fibrils is very similar to that of Alzheimers fibrils.
https://www.eurekalert.org/pub_releases/2020-06/fj-nio061520.php
Scientists take cool look at dendrite growth - Materials TodayScientists take cool look at dendrite growth - Materials Today
Scientists have used cryo-electron microscopy to capture the first atomic-level images of the crystalline dendrites that can grow in batteries.
https://www.materialstoday.com/characterization/news/scientists-take-cool-look-at-dendrite-growth/
Mapping the Structure and Function of the E1 and E2 Glycoproteins in Alphaviruses - CaltechAUTHORSMapping the Structure and Function of the E1 and E2 Glycoproteins in Alphaviruses - CaltechAUTHORS
The 9 Å resolution cryo-electron microscopy map of Sindbis virus presented here provides structural information on the polypeptide topology of the E2 protein, on the interactions between the E1 and E2 glycoproteins in the formation of a heterodimer, on the difference in conformation of the two types of trimeric spikes, on the interaction between the transmembrane helices of the E1 and E2 proteins, and on the conformational changes that occur when fusing with a host cell. The positions of various markers on the E2 protein established the approximate topology of the E2 structure. The largest conformational differences between the icosahedral surface spikes at icosahedral 3-fold and quasi-3-fold positions are associated with the monomers closest to the 5-fold axes. The long E2 monomers, containing the cell receptor recognition motif at their extremities, are shown to rotate by about 180^o and to move away from the center of the spikes during fusion. ...
https://authors.library.caltech.edu/63550/
Cryo-electron microscopy of viruses | Gelbart Lab
Cryo-electron microscopy (cryo-EM) is a structural technique that images biological macromolecules in native-like conditions, and has been widely applied to the study of viruses. Virus structures have been determined by cryo-EM at resolutions ranging from molecular (~30-50Angstrom) to near atomic (~4 angstrom).... ...
http://virus.chem.ucla.edu/biblio/cryo-electron-microscopy-viruses
First appointment in new Salk Fellows Program - Salk Institute for Biological StudiesFirst appointment in new Salk Fellows Program - Salk Institute for Biological Studies
Most fellows will come directly from a PhD or MD program and have expertise in a wide range of innovative technologies. Their work will have a combination of novelty, originality and risk, factors that often lower the chances of obtaining support through traditional channels. Salk Fellows will be independent group leaders and will be appointed for an initial period of three years, which may be followed by a two-year extension.. Salks first Fellow in this new program, Dmitry Lyumkis, joined the Salk on July 1 from The Scripps Research Institute and has made groundbreaking innovations in biological imaging. He uses single-particle cryo-electron microscopy-a cutting-edge technology that enables the visualization of large proteins and protein complexes under near-native conditions-to build three-dimensional models of the imaged objects. The resulting 3D models, which can now be resolved at near-atomic resolution with select specimens, facilitate a better understanding of protein function and ...
https://www.salk.edu/news-release/first-appointment-in-new-salk-fellows-program/
Cryo-EM structure of a licensed DNA replication origin | CrickCryo-EM structure of a licensed DNA replication origin | Crick
Eukaryotic origins of replication are licensed upon loading of the MCM helicase motor onto DNA. ATP hydrolysis by MCM is required for loading and the post-catalytic MCM is an inactive double hexamer that encircles duplex DNA. Origin firing depends on MCM engagement of Cdc45 and GINS to form the CMG holo-helicase. CMG assembly requires several steps including MCM phosphorylation by DDK. To understand origin activation, here we have determined the cryo-EM structures of DNA-bound MCM, either unmodified or phosphorylated, and visualize a phospho-dependent MCM element likely important for Cdc45 recruitment. MCM pore loops touch both the Watson and Crick strands, constraining duplex DNA in a bent configuration. By comparing our new MCM-DNA structure with the structure of CMG-DNA, we suggest how the conformational transition from the loaded, post-catalytic MCM to CMG might promote DNA untwisting and melting at the onset of replication. ...
https://www.crick.ac.uk/research/publications/cryo-em-structure-of-a-licensed-dna-replication-origin
Worlds leading supplier of electron microscopy solutions partners with Imaging CoE for R&D - Imaging CoEWorlds leading supplier of electron microscopy solutions partners with Imaging CoE for R&D - Imaging CoE
FEI have recently collaborated with the ARC Centre of Excellence in Advanced Molecular Imaging (Imaging CoE) to test a new approach to optimise samples prior to preparing them for vitrification and subsequent cryo-transmission electron microscopy (cryo-EM).
http://imagingcoe.org/fei-partnership-2015/
Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics - Fingerprint - University of...Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics - Fingerprint - University of...
Fingerprint Dive into the research topics of Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics. Together they form a unique fingerprint. ...
https://experts.illinois.edu/en/publications/mature-hiv-1-capsid-structure-by-cryo-electron-microscopy-and-all/fingerprints/
Phasing of the Triatoma virus diffraction data using a cryo-electron microscopy reconstruction. - Institut PasteurPhasing of the Triatoma virus diffraction data using a cryo-electron microscopy reconstruction. - Institut Pasteur
The blood-sucking reduviid bug Triatoma infestans, one of the most important vector of American human trypanosomiasis (Chagas disease) is infected by the Triatoma virus (TrV). TrV has been classified as a member of the Cripavirus genus (type cricket paralysis virus) in the Dicistroviridae family. This work presents the three-dimensional cryo-electron microscopy (cryo-EM) reconstruction of the TrV capsid at about 25 A resolution and its use as a template for phasing the available crystallographic data by the molecular replacement method. The main structural differences between the cryo-EM reconstruction of TrV and other two viruses, one from the same family, the cricket paralysis virus (CrPV) and the human rhinovirus 16 from the Picornaviridae family are presented and discussed.
https://hal-pasteur.archives-ouvertes.fr/pasteur-00335426
Cryo Electron Microscopy and Electron Tomography - Drug Discovery World (DDW)Cryo Electron Microscopy and Electron Tomography - Drug Discovery World (DDW)
Cryo electron microscopy and electron tomography will play a crucial role in the future of drug development.. The completion of the human genome project has focused tremendous interest in determining the structure and function of the tens of thousands of proteins that the genome encodes. Current structural determination techniques are difficult, time-consuming and not generally applicable to all proteins.. Cryo Electron Tomography (ET) is, by comparison, relatively straightforward and fast. While it does not achieve atomic scale resolution, it can resolve tertiary and quaternary structure - the level at which much of the critical interaction between proteins occurs. Because it does not require crystallisation, it is applicable to most proteins, including large proteins and protein complexes, flexible proteins and membrane proteins. Because it operates on a single protein molecule, it can provide information that is critical to drug development and unavailable from other techniques, for example, ...
https://www.ddw-online.com/cryo-electron-microscopy-and-electron-tomography-1155-200504/
Cryo-electron tomography of cells: connecting structure and function :: MPG.PuReCryo-electron tomography of cells: connecting structure and function :: MPG.PuRe
Author: Lucic, V. et al.; Genre: Journal Article; Published in Print: 2008-08; Keywords: cryo-electron tomography; correlative light microscopy; electron microscopy; Title: Cryo-electron tomography of cells: connecting structure and function
https://pure.mpg.de/pubman/faces/ViewItemOverviewPage.jsp?itemId=item_1579626
Combined Cryogenic Transmission Electron Microscopy and Electron Spin Resonance Studies of Egg Phosphatidylchloline Liposomes...Combined Cryogenic Transmission Electron Microscopy and Electron Spin Resonance Studies of Egg Phosphatidylchloline Liposomes...
Combined Cryogenic Transmission Electron Microscopy and Electron Spin Resonance Studies of Egg Phosphatidylchloline Liposomes Loaded with a Carboranyl Compound Intended for Boron Neutron Capture Therapy ...
http://uu.diva-portal.org/smash/record.jsf?pid=diva2:74380
Cryo-electron Microscopy Structure of the Native Prototype Foamy Virus Glycoprotein and Virus Architecture - Université...
Foamy viruses (FV) belong to the genus Spumavirus, which forms a distinct lineage in the Retroviridae family. Although the infection in natural hosts and zoonotic transmission to humans is asymptomatic, FVs can replicate well in human cells making it an attractive gene therapy vector candidate. Here we present cryo-electron microscopy and (cryo-)electron tomography ultrastructural data on purified prototype FV (PFV) and PFV infected cells. Mature PFV particles have a distinct morphology with a capsid of constant dimension as well as a less ordered shell of density between the capsid and the membrane likely formed by the Gag N-terminal domain and the cytoplasmic part of the Env leader peptide gp18LP. The viral membrane contains trimeric Env glycoproteins partly arranged in interlocked hexagonal assemblies. In situ 3D reconstruction by subtomogram averaging of wild type Env and of a Env gp48TM- gp80SU cleavage site mutant showed a similar spike architecture as well as stabilization of the hexagonal
https://hal.univ-grenoble-alpes.fr/hal-01406339
Cryo Electron Microscopy scoops Nobel Prize in Chemistry. | Department of Political StudiesCryo Electron Microscopy scoops Nobel Prize in Chemistry. | Department of Political Studies
The Nobel prize in chemistry has been awarded to the three scientists for developing a technique to produce images of the molecules of life frozen in time. The technique, called cryo-electron microscopy, allows biomolecules to be visualised in their natural configuration for the first time, triggering a revolution in biochemistry, according to the Nobel committee. The latest versions of the technology mean scientists can record biochemical processes as they unfold in film-like sequences.. Richard Henderson, a Scottish scientist and professor at the MRC Laboratory of Molecular Biology, was the first to successfully modify the electron microscope to image a protein involved in photosynthesis, by using a weaker beam and taking pictures from many angles. Joachim Frank, a German-born professor at Columbia University in New York, developed mathematical algorithms that allowed the method to be applied to a wider array of molecules. Jacques Dubochet, who is Swiss and an honorary professor at the ...
http://www.politics.uct.ac.za/cryo-electron-microscopy-scoops-nobel-prize-chemistry
Insights into bunyavirus architecture from electron cryotomography of Uukuniemi virusInsights into bunyavirus architecture from electron cryotomography of Uukuniemi virus
Bunyaviridae is a large family of viruses that have gained attention as emerging viruses because many members cause serious disease in humans, with an increasing number of outbreaks. These negative-strand RNA viruses possess a membrane envelope covered by glycoproteins. The virions are pleiomorphic and thus have not been amenable to structural characterization using common techniques that involve averaging of electron microscopic images. Here, we determined the three-dimensional structure of a member of the Bunyaviridae family by using electron cryotomography. The genome, incorporated as a complex with the nucleoprotein inside the virions, was seen as a thread-like structure partially interacting with the viral membrane. Although no ordered nucleocapsid was observed, lateral interactions between the two membrane glycoproteins determine the structure of the viral particles. In the most regular particles, the glycoprotein protrusions, or spikes, were seen to be arranged on an icosahedral ...
http://umu.diva-portal.org/smash/record.jsf?faces-redirect=true&language=en&searchType=SIMPLE&query=&af=%5B%5D&aq=%5B%5B%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&pid=diva2%3A408313&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all
Delmic Microscopy Blog | cryo-electron tomographyDelmic Microscopy Blog | cryo-electron tomography
cryo-electron tomography | This blog addresses the various applications and techniques to be discovered in the fields of cathodoluminescence and correlative light and electron microscopy.
https://blog.delmic.com/topic/cryo-electron-tomography
Rapid tilt-series method for cryo-electron tomography: Characterizing stage behavior during FISE acquisition - CaltechAUTHORSRapid tilt-series method for cryo-electron tomography: Characterizing stage behavior during FISE acquisition - CaltechAUTHORS
We and others recently developed rapid tilt-series acquisition methods for cryo-electron tomography on a Titan Krios G3i equipped with a single axis holder and a K-series direct electron detector and showed that one of these, the fast-incremental single exposure (FISE) method, significantly accelerates tilt-series acquisition when compared to traditional methods while preserving the quality of the images. Here, we characterize the behavior of our single axis holder in detail during a FISE experiment to optimally balance data quality with speed. We explain our methodology in detail so others can characterize their own stages, and conclude with recommendations for projects with different resolution goals. ...
https://authors.library.caltech.edu/103403/
Simulating cryo electron tomograms of crowded cell cytoplasm for assessment of automated particle picking | BMC Bioinformatics ...Simulating cryo electron tomograms of crowded cell cytoplasm for assessment of automated particle picking | BMC Bioinformatics ...
Cryo-electron tomography is an important tool to study structures of macromolecular complexes in close to native states. A whole cell cryo electron tomogram contains structural information of all its macromolecular complexes. However, extracting this information remains challenging, and relies on sophisticated image processing, in particular for template-free particle extraction, classification and averaging. To develop these methods it is crucial to realistically simulate tomograms of crowded cellular environments, which can then serve as ground truth models for assessing and optimizing methods for detection of complexes in cell tomograms. We present a framework to generate crowded mixtures of macromolecular complexes for realistically simulating cryo electron tomograms including noise and image distortions due to the missing-wedge effects. Simulated tomograms are then used for assessing the template-free Difference-of-Gaussian (DoG) particle-picking method to detect complexes of different shapes and
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-016-1283-3
Joachim Frank Wins Nobel Prize in ChemistryJoachim Frank Wins Nobel Prize in Chemistry
Now we can see the intricate details of the biomolecules in every corner of our cells, in every drop of our body fluids, says Sara Snogerup Linse, a Swedish scientist who chairs the Nobel committee for chemistry. Frank, who was born in Germany during World War II, received degrees from the Universities of Freiburg and Munich before earning a doctorate in physics from the Technical University of Munich in 1970. Over the next few decades, he held positions at a number of academic institutions in Germany, the United Kingdom, and the United States, all the while researching the computational-imaging methods that eventually made cryo-electron microscopy possible. He joined Columbia as a professor in the Department of Biochemistry and Molecular Biophysics and the Department of Biological Sciences in 2008. In his own research, Frank has used cryo-electron microscopy to investigate the interactions between ribosomes - which serve as the protein factories of the cell - and other molecules. In a 2013 ...
http://magazine.columbia.edu/print/2339
Structural studies of human immunodeficiency virus type 1 by cryo-electron tomography - CaltechTHESISStructural studies of human immunodeficiency virus type 1 by cryo-electron tomography - CaltechTHESIS
Gag, the major structural component of the type 1 human immunodeficiency virus (HIV-1), comprises the matrix (MA), capsid (CA), nucleocapsid (NC), and p6 proteins, as well as the SP1 and SP2 spacer peptides. In the immature HIV-1 virion, the domains of Gag are arranged radially with the amino-terminus of MA at the membrane. Mature viral particles are formed when Gag is proteolytically cleaved, allowing CA to reassemble into the viral core, which contains NC bound to genomic RNA. While the structures of nearly every HIV-1 protein are known in atomic detail from X-ray crystallography and NMR spectroscopy, many questions remain about the intermolecular interactions in both the immature and mature particles. We have obtained three-dimensional structures of individual immature and mature HIV-1 virus-like particles by cryoelectron tomography. Reconstructions of the mature particles revealed diverse core morphologies with a preference for conical shapes consistent with 5,7 fullerene cones. Uniform ...
https://thesis.library.caltech.edu/2459/
Bsoft: References
Dearborn AD, Wall JS, Cheng N, Heymann JB, Kajava AV, Varkey J, Langen R, Steven AC (2016) Alpha-synuclein amyloid fibrils with two entwined, asymmetrically associated protofibrils. J Biol Chem. 291(5): 2310 - 2318.. DiMattia MA, Watts NR, Cheng N, Huang R, Heymann JB, Grimes JM, Wingfield PT, Stuart DI, Steven AC (2016) The structure of hiv-1 rev filaments suggests a bilateral model for rev-rre assembly. Structure. 24(7): 1068 - 1080.. Fontana J, Cardone G, Heymann JB, Winkler DC and Steven AC (2012) Structural Changes in Influenza Virus at Low pH Characterized by Cryo-Electron Tomography. Journal of Virology, 86(6): 2919 - 2929.. Grünewald K, Desai P, Winkler DC, Heymann JB, Belnap DM, Baumeister W and Steven AC (2003). Three-dimensional structure of herpes simplex virus from cryo-electron tomography. Science 302(5649): 1396 - 1398.. Harris A, Cardone G, Winkler DC, Heymann JB, Brecher M, White JM, Steven AC (2006) Influenza virus pleiomorphy characterized by cryoelectron tomography. ...
https://lsbr.niams.nih.gov/bsoft/bsoft_ref.html
Electron Crystallography of Soluble and Membrane Proteins - beck-shop.deElectron Crystallography of Soluble and Membrane Proteins - beck-shop.de
The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment. Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, including protein sample preparation, 2D crystallization, and screening in negative stain over electron cryo-microscopy (cryo-EM) and data processing, as well as modeling of conformational changes. Additional chapters provide perspective on past, present, and future challenges as well as complementary methods. Written for the popular Methods in Molecular Biology™ series, the work contains the kind of detailed ...
http://m.beck-shop.de/item/3131303031323032
Cryoelectron tomography - Latest research and news | NatureCryoelectron tomography - Latest research and news | Nature
Oda and Yanagisawa report 3D cryo-electron tomography structures of Z-disc from porcine cardiac myofibrils in relaxed and activated states. They show that α-actinin dimers exhibit contraction dependent swinging and sliding motions in response to a global twist in the F-actin lattice. These findings provide insights into conformational changes of the Z-disc in the context of active myofibrils.. ...
https://www.nature.com/subjects/cryoelectron-tomography?error=cookies_not_supported&code=cb28e099-ba2b-4011-aa15-b133f5801f66
Structure of the full-length HCV IRES in solution
The 5-untranslated region of the hepatitis C virus genome contains an internal ribosome entry site (IRES) that initiates cap-independent translation of the viral RNA. Until now, the structural characterization of the entire (IRES) remained limited to cryo-electron microscopy reconstructions of the (IRES) bound to different cellular partners. Here we report an atomic model of free full-length hepatitis C virus (IRES) refined by selection against small-angle X-ray scattering data that incorporates the known structures of different fragments. We found that an ensemble of conformers reproduces small-angle X-ray scattering data better than a single structure suggesting in combination with molecular dynamics simulations that the hepatitis C virus (IRES) is an articulated molecule made of rigid parts that move relative to each other. Principal component analysis on an ensemble of physically accessible conformers of hepatitis C virus (IRES) revealed dominant collective motions in the molecule, which may
http://hal.univ-grenoble-alpes.fr/hal-01666137
Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM. | MRC Mitochondrial Biology Unit
Adenosine triphosphate (ATP), the chemical energy currency of biology, is synthesized in eukaryotic cells primarily by the mitochondrial ATP synthase. ATP synthases operate by a rotary catalytic mechanism where proton translocation through the membrane-inserted FO region is coupled to ATP synthesis in the catalytic F1 region via rotation of a central rotor subcomplex. We report here single particle electron cryomicroscopy (cryo-EM) analysis of the bovine mitochondrial ATP synthase. Combining cryo-EM data with bioinformatic analysis allowed us to determine the fold of the a subunit, suggesting a proton translocation path through the FO region that involves both the a and b subunits. 3D classification of images revealed seven distinct states of the enzyme that show different modes of bending and twisting in the intact ATP synthase. Rotational fluctuations of the c8-ring within the FO region support a Brownian ratchet mechanism for proton-translocation-driven rotation in ATP synthases.. ...
http://www.mrc-mbu.cam.ac.uk/publications/2468/structure-and-conformational-states-bovine-mitochondrial-atp-synthase-cryo-em
Protein structure could unlock new treatments for cystic fibrosis - Scienmag: Latest Science and Health NewsProtein structure could unlock new treatments for cystic fibrosis - Scienmag: Latest Science and Health News
The structure of a TMEM16 scramblase, which functions as a lipid transporter and plays an important role in blood coagulation, was already known from previous work. Researchers of the Department of Biochemistry at the University of Zurich have now also succeeded in decrypting the structure of the chloride channel TMEM16A. To do so, the team led by Professor Raimund Dutzler used cryo-electron microscopy (cryo-EM), a technique whose pioneers were recently awarded the Nobel Prize in Chemistry. The molecular architecture of this membrane protein is crucial for the targeted development of drugs for treating cystic fibrosis, emphasizes Dutzler.. Discovery of a novel activation mechanism. The chloride channel TMEM16A can be found in different organs of the body and plays a key role in the secretion of chloride in the lung, the contraction of smooth muscles, and the perception of pain. How its structure differs from closely related scramblases of the same family and how the protein is activated by ...
https://scienmag.com/protein-structure-could-unlock-new-treatments-for-cystic-fibrosis/
Capsids of tricorn protease studied by electron cryomicroscopy :: MPG.PuReCapsids of tricorn protease studied by electron cryomicroscopy :: MPG.PuRe
Author: Walz, J. et al.; Genre: Journal Article; Published in Print: 1999; Keywords: Electron microscopy; Icosahedral capsids; Image analysis; Thermoplasma.; Degradation; Microscopy; Resolution; Products.; Biochemistry & Biophysics in Current Contents(R)/Life Sciences.; Title: Capsids of tricorn protease studied by electron cryomicroscopy
http://pubman.mpdl.mpg.de/pubman/faces/viewItemFullPage.jsp?itemId=escidoc%3A1582434%3A1&view=EXPORT
Coacervation of Elastin-Like Recombinamer MicrogelsCoacervation of Elastin-Like Recombinamer Microgels
The investigation of the coacervation (self-aggregation) behavior of biomicrogels which can potentially be used as drug carriers is an important topic, because self-aggregation can not only cause loss of activity, but also toxicity and immunogenicity. To study this effect microgels from elastin-like recombinamer are synthesized using miniemulsion technique. The existence of coacervation for such microgels, at different concentrations and temperatures, is studied and proved by cryo-field emission scanning clectron microscopy (cryo-FESEM), cryo-transmission electron microscopy (cryo-TEM), and by a novel 1H high-resolution magic angle sample spinning (HRMAS), nuclear magnetic resonance (NMR) spectroscopy, and relaxometry methods. The findings by 1H HRMAS NMR spectroscopy and relaxometry show simultaneous processes of volume phase temperature transition and coacervation with different sensitivity for hydrophobic and hydrophilic amino acid side-chains in the microgel. The coacervation process is more ...
https://uvadoc.uva.es/handle/10324/24805
Cryo-EM structure of the small subunit of the mammalian mitochondrial ribosome. « RNomicsCryo-EM structure of the small subunit of the mammalian mitochondrial ribosome. « RNomics
Related Articles Cryo-EM structure of the small subunit of the mammalian mitochondrial ribosome. Proc Natl Acad Sci U S A. 2014 May 20;111(20):7284-9 Authors: Kaushal PS, Sharma MR, Booth TM, Haque EM, Tung CS, Sanbonmatsu KY, Spremulli LL, Agrawal RK Abstract The mammalian mitochondrial ribosomes (mitoribosomes) are responsible for synthesizing 13 membrane proteins that form…
https://rnomics.wordpress.com/2014/07/18/cryo-em-structure-of-the-small-subunit-of-the-mammalian-mitochondrial-ribosome/
Soft cellulose II nanospheres: sol gel behaviour, swelling and material synthesisBeaumont, Marco; Rosenfeldt, Sabine; Tardy,...Soft cellulose II nanospheres: sol gel behaviour, swelling and material synthesisBeaumont, Marco; Rosenfeldt, Sabine; Tardy,...
High axial aspect crystalline nanomaterials have emerged as polymeric building blocks for the construction of supermaterials. In contrast to this form, amorphous nanospheres have remained largely untapped. This is especially peculiar in the context of material assembly, due to the wide range of opportunities they offer by virtue of their soft particle characteristics, high volume ratio at low solid content and their highly swollen and accessible structure. In the context of cellulose, these colloids represent a new field in the family of nanocelluloses. We report an organic solvent-free, heterogeneous and simple synthesis of spherical carboxylated nanoparticles bearing a distinctive, amorphous outer shell structure. The particle shape is evaluated by atomic force microscopy, cryo-transmission electron microscopy, dynamic light scattering and small-angle X-ray scattering. The soft shell structure of the particles and their responsiveness to ionic strength and pH are quantified by the combination ...
https://www.xenocs.com/soft-cellulose-ii-nanospheres-sol%C2%96gel-behaviour-swelling-and-material-synthesisbeaumont-marco-rosenfeldt-sabine-tardy-blaise-l-gusenbauer-claudia-khakalo-alexey-nonappa-opietnik-ma/
Project abstract for group mccormic | Minnesota Supercomputing InstituteProject abstract for group mccormic | Minnesota Supercomputing Institute
Cryo-Electron Three-Dimensional Imaging of Soft Materials. Cryogenic transmission electron microscopy (Cryo-TEM) is used to characterize labile systems that are not allowed in room temperature electron microscopy due to a high vapor pressure. Although soft materials are characterized with cryo-TEM, the original structure of the sample is lost. This is because a three-dimensional (3D) structure is projected to a two-dimensional (2D) image. These researchers used a single-particle construction method to visualize conventional 2D images to 3D microstructures with a high resolution. They are exploring an electron tomography method to construct 3D images. This method combines a series of 2D images that are collected at different angles and constructs a 3D image. To do this, mathematical algorithms of cross-correlation and an image alignment must be combined and solved simultaneously. Computer calculations are indispensable in structure characterization study and allow researchers to understand the ...
https://www.msi.umn.edu/project-abstract/mccormic/Cryo-Electron%203D%20Imaging%20of%20Soft%20Materials%20%20
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo - Oxford Particle...
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https://www.opic.ox.ac.uk/publications/487909/
Ariane Briegel - Leiden UniversityAriane Briegel - Leiden University
I am professor in electron cryotomography and bacterial chemotaxis. I am interested in understanding how microbes sense and respond to their environment. In order to gain insight into the structure and function of the molecular complexes involved in these behaviors, my lab uses electron cryotomography (ECT). This technique allows us to directly study microbes in their native state at resolutions capable of visualizing individual proteins.
https://www.universiteitleiden.nl/en/staffmembers/ariane-briegel
Cryo-EM reconstruction of the giant Mimivirus using C5 symmetry | NIH 3D Print ExchangeCryo-EM reconstruction of the giant Mimivirus using C5 symmetry | NIH 3D Print Exchange
Mimivirus, Acanthamoeba polyphaga, cryo-electron microscope, Atomic Force Microscope, fiber, DIGESTION, starfish-shaped feature, 5-fold symmetry, nucleocapsid, major capsid ...
https://3dprint.nih.gov/discover/3dpx-015801
Viruses | Free Full-Text | Fluorescent Protein-Tagged Sindbis Virus E2 Glycoprotein Allows Single Particle Analysis of Virus...Viruses | Free Full-Text | Fluorescent Protein-Tagged Sindbis Virus E2 Glycoprotein Allows Single Particle Analysis of Virus...
Sindbis virus (SINV) is an enveloped, mosquito-borne alphavirus. Here we generated and characterized a fluorescent protein-tagged (FP-tagged) SINV and found that the presence of the FP-tag (mCherry) affected glycoprotein transport to the plasma membrane whereas the specific infectivity of the virus was not affected. We examined the virions by transmission electron cryo-microscopy and determined the arrangement of the FP-tag on the surface of the virion. The fluorescent proteins are arranged icosahedrally on the virus surface in a stable manner that did not adversely affect receptor binding or fusion functions of E2 and E1, respectively. The delay in surface expression of the viral glycoproteins, as demonstrated by flow cytometry analysis, contributed to a 10-fold reduction in mCherry-E2 virus titer. There is a 1:1 ratio of mCherry to E2 incorporated into the virion, which leads to a strong fluorescence signal and thus facilitates single-particle tracking experiments. We used the FP-tagged virus for high
http://www.mdpi.com/1999-4915/7/12/6182
Horizontal membrane-intrinsic α-helices in the stator a-subunit of an F-type ATP synthase. | MRC Mitochondrial Biology Unit
ATP, the universal energy currency of cells, is produced by F-type ATP synthases, which are ancient, membrane-bound nanomachines. F-type ATP synthases use the energy of a transmembrane electrochemical gradient to generate ATP by rotary catalysis. Protons moving across the membrane drive a rotor ring composed of 8-15 c-subunits. A central stalk transmits the rotation of the c-ring to the catalytic F1 head, where a series of conformational changes results in ATP synthesis. A key unresolved question in this fundamental process is how protons pass through the membrane to drive ATP production. Mitochondrial ATP synthases form V-shaped homodimers in cristae membranes. Here we report the structure of a native and active mitochondrial ATP synthase dimer, determined by single-particle electron cryomicroscopy at 6.2 Å resolution. Our structure shows four long, horizontal membrane-intrinsic α-helices in the a-subunit, arranged in two hairpins at an angle of approximately 70° relative to the c-ring ...
http://www.mrc-mbu.cam.ac.uk/publications/2697/horizontal-membrane-intrinsic-%CE%B1-helices-stator-subunit-f-type-atp-synthase
First atomic model of human telomerase constructed by electron cryo-microscopyFirst atomic model of human telomerase constructed by electron cryo-microscopy
Telomeres are large nucleoproteins structures that cap the ends of chromosomes in eukaryotic cells. When a cell divides, a small portion of the telomere is lost due to the inherently incomplete process of genome replication. If left unchecked, over time the telomeres will reach a critically short length and the cell will face genomic instability, deterioration or death. To offset this shortening, an essential enzyme called telomerase rebuilds the telomeres by synthesizing new telomeric DNA repeats at chromosome ends. Kelly Nguyens group, in the LMBs Structural Studies Division, has solved the first complete atomic model of this enzyme and discovered a histone dimer as novel telomerase subunits.
https://phys.org/news/2021-04-atomic-human-telomerase-electron-cryo-microscopy.html
Dirksen E. Bussiere | ACS Division of Biological Chemistry WebsiteDirksen E. Bussiere | ACS Division of Biological Chemistry Website
December 9th, 2019 by Dirksen E. Bussiere. Nature Chemical Biology, Published online: 09 December 2019; doi:10.1038/s41589-019-0411-6. The crystal and cryo-electron microscopy structure analysis of the DCAF15-DDB1-DDA1-indisulam-RBM39 complex revealed the detailed mechanism of action of indisulam-induced RBM39 degradation and defined an α-helical degron motif in RBM39 ...
http://www.divbiolchem.org/author/dirksen-e-bussiere/
The Stephen Long Lab: Publications | Sloan Kettering InstituteThe Stephen Long Lab: Publications | Sloan Kettering Institute
Miller, A. N.*, Vaisey, G.*, & Long, S. B. (2019). Molecular mechanisms of gating in the calcium-activated chloride channel bestrophin. eLife (Abstract , open state Cryo-EM map , Atomic Coordinates). *A. N. Miller and G. Vaisey are co-first authors.. Vaisey, G., & Long, S. B. (2018). An allosteric mechanism of inactivation in the calcium-dependent chloride channel BEST1. The Journal of General Physiology, jgp.201812190. (Abstract). Hou, X., Burstein, S. R., & Long, S. B. (2018). Structures reveal opening of the store-operated calcium channel Orai. eLife, 7. (Manuscript , Atomic Coordinates). Baradaran, R., Wang, C., Siliciano, A. F., & Long, S. B. (2018). Cryo-EM structures of fungal and metazoan mitochondrial calcium uniporters. Nature, 559(7715), 580-584. (Abstract , Atomic Coordinates , Cryo-EM map). Melinda Diver, Leanne Pedi, Akiko Koide, Shohei Koide, Stephen B. Long (2018). Atomic structure of the eukaryotic intramembrane RAS methyltransferase ICMT. Nature. Jan 25; 553(7689):526-529. ...
https://www.mskcc.org/research/ski/labs/stephen-long/publications
Freezing immunoglobulins to see them moveFreezing immunoglobulins to see them move
The issue of protein dynamics and its implications in the biological function of proteins are arousing greater and greater interest in different fields of molecular biology. In cryo-electron tomography experiments one may take several snapshots of a given biological macromolecule. In principle, a large enough collection of snapshots of the molecule may then be used to calculate its equilibrium configuration in terms of the experimentally accessible degrees of freedom and, hence, to estimate its potential energy. This information would be crucial in order to analyze the biological functions of biomolecules by directly accessing the relevant dynamical indicators. In this article, we analyze the results of cryo-electron tomography experiments performed on monoclonal murine IgG2a antibodies. We measure the equilibrium distribution of the molecule in terms of the relevant angular coordinates and build a mechanical model of the antibody dynamics. This approach enables us to derive an explicit ...
https://infoscience.epfl.ch/record/147438
Virus caught in the act of infecting a cellVirus caught in the act of infecting a cell
For example, the researchers now know that most of the fibers are usually bound to the virus head rather than extended, as was previously thought. That those fibers are in a dynamic equilibrium between bound and extended states is also new. Molineux said that the idea that phages walk over the cell surface was previously proposed, but their paper provides the first experimental evidence that this is the case. This is also the first time that scientists have made actual images showing how the viruss tail extends into the host - the very action that allows it to infect a cell with its DNA.. I first hypothesized that T7 made an extended tail more than 10 years ago, said Molineux, but this is the first irrefutable experimental evidence for the idea and provides the first images of what it looks like.. The researchers used a combination of genetics and cryo-electron tomography to image the infection process. Cryo-electron tomography is a process similar to a CT scan, but it is scaled to study ...
http://www.innovations-report.com/html/reports/life-sciences/virus-caught-act-infecting-a-cell-207890.html
Spatial Localization of the Ebola Virus Glycoprotein Mucin-Like Domain Determined by Cryo-Electron TomographySpatial Localization of the Ebola Virus Glycoprotein Mucin-Like Domain Determined by Cryo-Electron Tomography
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
https://www.growkudos.com/publications/10.1128%25252Fjvi.00870-14/reader
HIV-1 Biogenesis Studied by Cellular Cryo-Electron Tomography and Biochemical in vitro Reconstitution - ORA - Oxford University...HIV-1 Biogenesis Studied by Cellular Cryo-Electron Tomography and Biochemical in vitro Reconstitution - ORA - Oxford University...
The file(s) for this record are currently under an embargo. If you complete the attached form, we can attempt to contact the author and ask if they are willing to let us send you a copy for your personal research use only. We will then pass this form and your request on to the author and let you know their response. ...
https://ora.ox.ac.uk/objects/uuid:eedd38f6-0deb-45e7-a59a-9a3b35c202ed
Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations -...
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https://www.immunology.ox.ac.uk/publications/1081854
Glycoprotein Ib clustering in platelets can be inhibited by α-linolenic acid as revealed by cryo-electron tomography |...Glycoprotein Ib clustering in platelets can be inhibited by α-linolenic acid as revealed by cryo-electron tomography |...
ALA is a plant-derived n-3 FA readily available in certain plant oils such as flaxseed, soybean and canola oil. Epidemiological studies have shown an inverse correlation between dietary ALA and cardiovascular events,11,30,31 although the molecular mechanisms of this protection are not completely known. Our group has investigated the molecular basis of several cardio-protective effects of ALA, showing that at least some of its effects are mediated by its action on endothelial cells, leukocytes and platelets.9,10,19 In this study, we have focused in particular on platelet adhesion to vWF under high-shear conditions, which represents the first step mediating platelet activation under arterial flow and is especially important in stenosed (atherosclerotic) arteries, where shear can reach extremely high values (,5,000 s−1).7,32,33. Here we show for the first time that ALA is able to partially inhibit platelet adhesion to vWF under a shear flow of 10,000 s−1, when whole blood is pre-incubated for 1 ...
http://www.haematologica.org/node/85732.full.print
Conveyor technology: Non-Contact Optical Vibration Measurement with Subnanometer ResolutionConveyor technology: Non-Contact Optical Vibration Measurement with Subnanometer Resolution
The new LSV laser-interferometric vibrometer is the ideal instrument for accurate, non-contact determination of temporal changes in the positions of objects or surfaces of arbitrary roughness
http://conveyor-tech.com/press/catalog/93/index.html
Multivariate Statistical Analysis of Large Datasets: Single Particle Electron MicroscopyMultivariate Statistical Analysis of Large Datasets: Single Particle Electron Microscopy
Biology is a challenging and complicated mess. Understanding this challenging complexity is the realm of the biological sciences: Trying to make sense of the massive, messy data in terms of discovering patterns and revealing its underlying general rules. Among the most powerful mathematical tools for organizing and helping to structure complex, heterogeneous and noisy data are the tools provided by multivariate statistical analysis (MSA) approaches. These eigenvector/eigenvalue data-compression approaches were first introduced to electron microscopy (EM) in 1980 to help sort out different views of macromolecules in a micrograph. After 35 years of continuous use and developments, new MSA applications are still being proposed regularly. The speed of computing has increased dramatically in the decades since their first use in electron microscopy. However, we have also seen a possibly even more rapid increase in the size and complexity of the EM data sets to be studied. MSA computations had thus become a
https://www.scirp.org/html/13-1240624_70260.htm
Structural properties of AMP-activated protein kinase: dimerization, molecular shape, and changes upon ligand binding.
Heterotrimeric AMP-activated protein kinase (AMPK) is crucial for energy homeostasis of eukaryotic cells and organisms. Here we report on (i) bacterial expression of untagged mammalian AMPK isoform combinations, all containing gamma(1), (ii) an automated four-dimensional purification protocol, and (iii) biophysical characterization of AMPK heterotrimers by small angle x-ray scattering in solution (SAXS), transmission and scanning transmission electron microscopy (TEM, STEM), and mass spectrometry (MS). AMPK in solution at low concentrations (~1 mg/ml) largely consisted of individual heterotrimers in TEM analysis, revealed a precise 1:1:1 stoichiometry of the three subunits in MS, and behaved as an ideal solution in SAXS. At higher AMPK concentrations, SAXS revealed concentration-dependent, reversible dimerization of AMPK heterotrimers and formation of higher oligomers, also confirmed by STEM mass measurements. Single particle reconstruction and averaging by SAXS and TEM, respectively, revealed similar
http://www.hal.inserm.fr/inserm-00390926
Magnetic imaging with a Zernike-type phase plate in a transmission electron microscope
We demonstrate the use of a hole-free phase plate (HFPP) for magnetic imaging in transmission electron microscopy by mapping the domain structure in PrDyFeB samples. The HFPP, a Zernike-like imaging method, allows for detecting magnetic signals in-focus to correlate the sample crystal structure and defects with the local magnetization topography, and to evidence stray fields protruding from the sample. Experimental and simulated results are shown and are compared with conventional Fresnel (out-of-focus) images without a phase plate. A key advantage of HFPP imaging is that the technique is free from the reference wave distortion from long-range fields affecting electron holography ...
http://connection.ebscohost.com/c/articles/87655875/magnetic-imaging-zernike-type-phase-plate-transmission-electron-microscope
Undergraduate | Stanford EEUndergraduate | Stanford EE
Cryo-Electron Microscopy (cryo-EM) is an imaging technology that is revolutionizing structural biology; the Nobel Prize in Chemistry 2017 was recently awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution.. Cryo-electron microscopes produce a large number of very noisy two-dimensional projection images of individual frozen molecules. Unlike related methods, such as computed tomography (CT), the viewing direction of each image is unknown. The unknown directions, together with extreme levels of noise and additional technical factors, make the determination of the structure of molecules challenging.. While other methods for structure determination, such as x-ray crystallography and nuclear magnetic resonance (NMR), measure ensembles of molecules, cryo-electron microscopes produce images of individual molecules. Therefore, cryo-EM could potentially be used to study ...
https://ee.stanford.edu/events/undergraduate
Ribosome and ribosomal RNA Structure : An experimental and computational analysis of expansion segments in eukaryotic ribosomal...Ribosome and ribosomal RNA Structure : An experimental and computational analysis of expansion segments in eukaryotic ribosomal...
Ribosomes are large ribonucleoprotein complexes which incorporate amino acids into peptide chains during translational process in all types of living cells. The eukaryotic ribosome is larger compared to its prokaryotic counterpart. The size differences are due to a larger protein part and that the rRNA contains eukaryote specific expansion segments (ES). Cryo-EM reconstruction has visualized many ES on the ribosomal surface which have given clues about function and structural features. However, the secondary structures of most ES are unknown or ill defined. In this thesis, the secondary and also to a certain extent the tertiary structures of several ES are determined by using computational methods and biochemical experimental techniques. The juxtaposition of ES6 close to ES3 in the Cryo-EM image of the yeast ribosome suggested that ES3 and ES6 might interact. A computational analysis of more than 2900 sequences shows that a complementary helical region of seven to nine contiguous base pairs can ...
http://su.diva-portal.org/smash/record.jsf?pid=diva2:198453
Colloquia - MathColloquia - Math
Title: Inverse Problems and Unsupervised Learning with applications to Cryo-Electron Microscopy (cryo-EM) Abstract: Cryo-EM is an imaging technology that is revolutionizing structural biology; the Nobel Prize in Chemistry 2017 was recently awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution. Cryo-electron microscopes produce a large number of very noisy two-dimensional projection images of individual frozen molecules. Unlike related methods, such as computed tomography (CT), the viewing direction of each image is unknown. The unknown directions, together with extreme levels of noise and additional technical factors, make the determination of the structure of molecules challenging. While other methods for structure determination, such as x-ray crystallography and nuclear magnetic resonance (NMR), measure ensembles of molecules together, cryo-EM produces measurements ...
https://www.math.wisc.edu/wiki/index.php?title=Colloquia&curid=277&oldid=14633
Colloquia - MathColloquia - Math
Title: Inverse Problems and Unsupervised Learning with applications to Cryo-Electron Microscopy (cryo-EM) Abstract: Cryo-EM is an imaging technology that is revolutionizing structural biology; the Nobel Prize in Chemistry 2017 was recently awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution. Cryo-electron microscopes produce a large number of very noisy two-dimensional projection images of individual frozen molecules. Unlike related methods, such as computed tomography (CT), the viewing direction of each image is unknown. The unknown directions, together with extreme levels of noise and additional technical factors, make the determination of the structure of molecules challenging. While other methods for structure determination, such as x-ray crystallography and nuclear magnetic resonance (NMR), measure ensembles of molecules together, cryo-EM produces measurements ...
http://www.math.wisc.edu/wiki/index.php?title=Colloquia&direction=prev&oldid=14710
Transmission electron cryomicroscopyTransmission electron cryomicroscopy
Presentation on Cryoelectron Microscopy , PharmaXChange.info Shi D, Nannenga BL, Iadanza MG, Gonen T (November 2013). "Three- ... Adrian M, Dubochet J, Lepault J, McDowall AW (1984). "Cryo-electron microscopy of viruses". Nature. 308 (5954): 32-6. Bibcode: ... Murata K, Wolf M (Feb 2018). "Cryo-electron microscopy for structural analysis of dynamic biological macromolecules". ... "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution". The ...
https://en.wikipedia.org/wiki/Transmission_electron_cryomicroscopy
SnRNPSnRNP
Several human and yeast snRNP structures were determined by the cryo-electron microscopy and successive single particle ... Stark, Holger; Reinhard Lührmann (2006). "Cryo-Electron Microscopy of Spliceosomal Components". Annual Review of Biophysics and ... The structures determined by single particle electron microscopy analysis are: human U1 snRNP, human U11/U12 di-snRNP, human U5 ...
https://en.wikipedia.org/wiki/SnRNP
Deborah F. KellyDeborah F. Kelly
"Cryo-Electron Microscopy in Cancer Research". Accelerating Microscopy. 2021-09-16. Retrieved 2022-04-08. "Celebrating Women in ... "Microchip-Based Toolkit to Complement Protein Analysis Using Cryo-Electron Microscopy - Nanoscale & Nanoscale Advances Blog". ... "About MSA - Council". Microscopy Society of America. Retrieved 2022-04-08. (Articles with short description, Short description ... Kelly was elected President of the Microscopy Society of America in 2022. Merryn Voysey; Sue Ann Costa Clemens; Shabir A Madhi ...
https://en.wikipedia.org/wiki/Deborah_F._Kelly
Lorena S. BeeseLorena S. Beese
X-ray crystallography and cryo-electron microscopy; structure-based drug design; protein-protein and protein-nucleic acid ...
https://en.wikipedia.org/wiki/Lorena_S._Beese
CryofixationCryofixation
Dubochet J, Adrian M, Chang JJ, Homo JC, Lepault J, McDowall AW, Schulz P (1988). "Cryo-electron microscopy of vitrified ... of biological materials as the first step in specimen preparation for electron microscopy and cryo-electron microscopy. Typical ... "The construction and operation of a simple and inexpensive slam freezing device for electron microscopy". Journal of Microscopy ... Ryan, Keith P. (1992). "Cryofixation of tissues for electron microscopy: a review of plunge cooling methods" (PDF). Scan. ...
https://en.wikipedia.org/wiki/Cryofixation
Amorphous iceAmorphous ice
... is used in some scientific experiments, especially in cryo-electron microscopy of biomolecules. The individual ... "Cryo-electron microscopy of vitrified specimens" (PDF). Quarterly Reviews of Biophysics. 21 (2): 129-228. doi:10.1017/ ...
https://en.wikipedia.org/wiki/Amorphous_ice
4D scanning transmission electron microscopy4D scanning transmission electron microscopy
"A Primer to Single-Particle Cryo-Electron Microscopy". Cell. 161 (3): 438-449. doi:10.1016/j.cell.2015.03.050. PMC 4409659. ... 4D scanning transmission electron microscopy (4D STEM) is a subset of scanning transmission electron microscopy (STEM) which ... Electron beam induced deposition Transmission electron microscopy (TEM) Scanning Transmission Electron Microscopy (STEM) Energy ... Scanning confocal electron microscopy (SCEM) Scanning electron microscope (SEM) Detectors for transmission electron microscopy ...
https://en.wikipedia.org/wiki/4D_scanning_transmission_electron_microscopy
Detectors for transmission electron microscopyDetectors for transmission electron microscopy
"A Primer to Single-Particle Cryo-Electron Microscopy". Cell. 161 (3): 438-449. doi:10.1016/j.cell.2015.03.050. ISSN 0092-8674. ... "Design and Characterization of 64 MegaPixel Fiber Optic Coupled CMOS Detector for Transmission Electron Microscopy". Microscopy ... "High Dynamic Range Pixel Array Detector for Scanning Transmission Electron Microscopy". Microscopy and Microanalysis. 22 (1): ... There are two main types of direct electron detector, both of which were first introduced to electron microscopy in the 2000s. ...
https://en.wikipedia.org/wiki/Detectors_for_transmission_electron_microscopy
Joachim FrankJoachim Frank
He is regarded as the founder of single-particle cryo-electron microscopy (cryo-EM), for which he shared the Nobel Prize in ... "Nobel Prize in Chemistry Awarded for Cryo-Electron Microscopy". The New York Times. October 4, 2017. Retrieved October 4, 2017 ... "The 16th Annual Wiley Prize in Biomedical Sciences Awarded for Pioneering Developments in Electron Microscopy". newsroom.wiley. ... Frank, Joachim (2009). "Single-particle reconstruction of biological macromolecules in electron microscopy -- 30 years". Q. Rev ...
https://en.wikipedia.org/wiki/Joachim_Frank
Electron cryotomographyElectron cryotomography
Zhang, Peijun (2013-10-01). "Correlative cryo-electron tomography and optical microscopy of cells". Current Opinion in ... One solution is to apply correlated cryo-fluorescence light microscopy, and even super-resolution light microscopy (e.g. cryo- ... Electron microscopy Electron tomography Transmission electron cryomicroscopy Transmission electron microscopy Gan, Lu; Jensen, ... "Cryo-electron microscopy of vitreous sections". The EMBO Journal. 23 (18): 3583-3588. doi:10.1038/sj.emboj.7600366. ISSN 0261- ...
https://en.wikipedia.org/wiki/Electron_cryotomography
EM Data BankEM Data Bank
Cryo-Electron Microscopy Colocalization Benchmark Source Lawson, Catherine L; Baker, Matthew L; Best, Christoph; et al. ( ... "Electron Microscopy Data Bank". Electron Microscopy Data Bank. Retrieved 2021-08-05. Esquivel-Rodríguez, J; Xiong, Y; Han, X; ... Software tools for molecular microscopy EM Data Bank US and UK Search pages Japan Search page (EM Navigator in PDBj) (Articles ... The EM Data Bank or Electron Microscopy Data Bank (EMDB) collects 3D EM maps and associated experimental data determined using ...
https://en.wikipedia.org/wiki/EM_Data_Bank
Jacques DubochetJacques Dubochet
Dubochet developed technologies in cryo-electron microscopy, cryo-electron tomography and cryo-electron microscopy of vitreous ... "Nobel Prize in Chemistry Awarded for Cryo-Electron Microscopy". The New York Times. 4 October 2017. Retrieved 4 October 2017. ... Dubochet, J.; McDowall, A. W. (December 1981). "Vitrification of pure water for electron microscopy". Journal of Microscopy. ... Blog Jacques Dubochet on Nobelprize.org including the Nobel Lecture on 8 December 2017 Early cryo-electron microscopy Portals: ...
https://en.wikipedia.org/wiki/Jacques_Dubochet
SmallpoxSmallpox
"Structure of intracellular mature vaccinia virus observed by cryoelectron microscopy". Journal of Virology. 68 (3): 1935-41. ... All orthopoxviruses exhibit identical brick-shaped virions by electron microscopy. If particles with the characteristic ...
https://en.wikipedia.org/wiki/Smallpox
Fusion mechanismFusion mechanism
"Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer". Nature. 531 (7592): 114-117. Bibcode:2016Natur. ...
https://en.wikipedia.org/wiki/Fusion_mechanism
Ronny HadaniRonny Hadani
Viewing angle classification of cryo-electron microscopy images using eigenvectors, A Singer, Z Zhao, Y Shkolnisky, R Hadani, ... Singer, A.; Zhao, Z.; Shkolnisky, Y.; Hadani, R. (2011-01-01). "Viewing Angle Classification of Cryo-Electron Microscopy Images ... Ronny Hadani Representation Theoretic Patterns in 3D Cryo-Electron Microscopy - Prof. Ronny Hadani (CS1 maint: url-status, ... "Representation theoretic patterns in three dimensional Cryo-Electron Microscopy I: The intrinsic reconstitution algorithm". ...
https://en.wikipedia.org/wiki/Ronny_Hadani
Single particle analysisSingle particle analysis
Using cryo-electron microscopy it has become possible to generate reconstructions with sub-nanometer resolution and near-atomic ... "Towards atomic resolution structural determination by single-particle cryo-electron microscopy". Current Opinion in Structural ... Transmission electron microscopy images are projections of the object showing the distribution of density through the object, ... EM Data Bank (EM Data Bank) Frank, Joachim (2006). Three-dimensional electron microscopy of macromolecular assemblies: ...
https://en.wikipedia.org/wiki/Single_particle_analysis
Epithelial sodium channelEpithelial sodium channel
"Structure of the human epithelial sodium channel by cryo-electron microscopy". eLife. 7: e39340. doi:10.7554/eLife.39340. PMC ... "Structure of the human epithelial sodium channel by cryo-electron microscopy". eLife. 7: e39340. doi:10.7554/eLife.39340. PMC ...
https://en.wikipedia.org/wiki/Epithelial_sodium_channel
ChlorosomeChlorosome
Cryo-electron microscopy was used to take pictures of the chlorosomes. The images reveal that the chlorophyll molecules inside ... Genetic techniques to create a mutant bacterium with a more regular internal structure, cryo-electron microscopy to identify ... The combination of NMR, cryo-electron microscopy and modeling enabled the scientists to determine that the chlorophyll ... "Determination of the topography and biometry of chlorosomes by atomic force microscopy". Photosynthesis Research. 71 (1-2): 83- ...
https://en.wikipedia.org/wiki/Chlorosome
FolditFoldit
November 2019). "Building de novo cryo-electron microscopy structures collaboratively with citizen scientists". PLOS Biology. ...
https://en.wikipedia.org/wiki/Foldit
Bacteriophage pRNABacteriophage pRNA
2001). "Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi29". J. Struct. Biol. 135 (1): ...
https://en.wikipedia.org/wiki/Bacteriophage_pRNA
Protein structureProtein structure
For larger protein complexes, cryo-electron microscopy can determine protein structures. The resolution is typically lower than ... cryo electron microscopy (cryo-EM) and dual polarisation interferometry to determine the structure of proteins. Protein ...
https://en.wikipedia.org/wiki/Protein_structure
ProteinProtein
Since then, cryo-electron microscopy (cryo-EM) of large macromolecular assemblies has been developed. Cryo-EM uses protein ... Cryoelectron microscopy is used to produce lower-resolution structural information about very large protein complexes, ... Zhou ZH (April 2008). "Towards atomic resolution structural determination by single-particle cryo-electron microscopy". Current ... This technique also uses an antibody to the protein of interest, along with classical electron microscopy techniques. The ...
https://en.wikipedia.org/wiki/Protein
Kiyoshi NagaiKiyoshi Nagai
From 2014, Nagai's group used cryo-electron microscopy to study the spliceosome. Structures of the U5.U4/U6 tri-snRNP gave the ... Fica, Sebastian M.; Nagai, Kiyoshi (2017-10-05). "Cryo-electron microscopy snapshots of the spliceosome: structural insights ... Insights from Cryo-Electron Microscopy Structures". Chemical Reviews. 118 (8): 4156-4176. doi:10.1021/acs.chemrev.7b00499. ISSN ...
https://en.wikipedia.org/wiki/Kiyoshi_Nagai
Cation diffusion facilitatorCation diffusion facilitator
2013) used cryoelectron microscopy to determine a 13 Å resolution structure of a YiiP homolog from Shewanella oneidensis within ... "Inward-facing conformation of the zinc transporter YiiP revealed by cryoelectron microscopy". Proceedings of the National ...
https://en.wikipedia.org/wiki/Cation_diffusion_facilitator
Electron microscopeElectron microscope
An entire field called cryo-electron microscopy has branched from this technique. With the development of cryo-electron ... "Vitrification of cryoelectron microscopy specimens revealed by high-speed photographic imaging". Journal of Microscopy. 211 (1 ... Adrian, Marc; Dubochet, Jacques; Lepault, Jean; McDowall, Alasdair W. (1984). "Cryo-electron microscopy of viruses". Nature ( ... Danilatos, G.D. (1986). "Environmental scanning electron microscopy in colour". Journal of Microscopy. 142: 317-325. doi: ...
https://en.wikipedia.org/wiki/Electron_microscope
Michael RossmannMichael Rossmann
Using cryo-electron microscopy, in 2016 his lab reported the first known structure of the Zika virus, responsible for a severe ... using a combination of cryo-electron microscopy and X-ray crystallography. Rossmann also had a long-term interest in ... "Visualization of membrane protein domains by cryo-electron microscopy of dengue virus". Nature Structural Biology. 10 (11): 907 ...
https://en.wikipedia.org/wiki/Michael_Rossmann
SapovirusSapovirus
"Antigenic and Cryo-Electron Microscopy Structure Analysis of a Chimeric Sapovirus Capsid". Journal of Virology. 90 (5): 2664- ... Assembly of new virus particles and release by cell lysis." Using electron microscopy, the sapovirus was first seen in ...
https://en.wikipedia.org/wiki/Sapovirus
DNA nanotechnologyDNA nanotechnology
... transmission electron microscopy and cryo-electron microscopy are often used in this case. Extended three-dimensional lattices ... The proposed nanoparticle was characterized by atomic force microscopy (AFM), transmission electron microscopy (TEM) and ... Nucleic acid structures can be directly imaged by atomic force microscopy, which is well suited to extended two-dimensional ... "Designed two-dimensional DNA Holliday junction arrays visualized by atomic force microscopy". Journal of the American Chemical ...
https://en.wikipedia.org/wiki/DNA_nanotechnology
Fourier shell correlationFourier shell correlation
In cryo-electron microscopy, the two volumes are the result of two three-dimensional reconstructions, each based on half of the ... The FSC originated in cryo-electron microscopy and gradually proliferated to other fields. To measure the FSC, two ... Frank, J. (2006). Three-Dimensional Electron Microscopy of Macromolecular Assemblies. New York: Oxford University Press. ISBN 0 ... "The Electron Microscopy Data Bank". www.ebi.ac.uk. Retrieved 2019-01-07. Harauz, G.; van Heel M. (1986). "Exact filters for ...
https://en.wikipedia.org/wiki/Fourier_shell_correlation
DensovirinaeDensovirinae
... determined by cryo-electron microscopy". Journal of Molecular Biology. 347 (4): 791-801. doi:10.1016/j.jmb.2005.02.009. PMID ...
https://en.wikipedia.org/wiki/Densovirinae
Bacillus virus phi29Bacillus virus phi29
employed cryo-electron microscopy to determine that, in vivo, only a pentamer or smaller polymer could spatially fit in the ... but these studies had a solely genetic basis rather than a microscopy based approach. In the year 2000, a study by Simpson et ...
https://en.wikipedia.org/wiki/Bacillus_virus_phi29
Klaus SchultenKlaus Schulten
A practical guide to combine cryo-electron microscopy and X-ray crystallography". Methods. 49 (2): 174-180. doi:10.1016/j.ymeth ... using molecular dynamics in combination with cryo-electron microscopy and X-ray crystallography. to study the structures of ... "Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics". Nature. 497 (7451): 643-6. Bibcode: ...
https://en.wikipedia.org/wiki/Klaus_Schulten
TelomeraseTelomerase
Two years later, the first cryo-electron microscopy (cryo-EM) structure of telomerase holoenzyme (Tetrahymena) was determined. ... The negative stain electron microscopy (EM) structures of human and Tetrahymena telomerases were characterized in 2013. ...
https://en.wikipedia.org/wiki/Telomerase
PicornavirusPicornavirus
... using X-ray crystallography and cryoelectron microscopy. Depending on the type and degree of dehydration, the viral particle is ...
https://en.wikipedia.org/wiki/Picornavirus
Spectral signal-to-noise ratioSpectral signal-to-noise ratio
The SSNR is a popular method for finding the resolution of a class average in cryo-electron microscopy. S S N R ( r ) = ∑ r i ... Frank, Joachim (2006). Three-Dimnsional Electron Microscopy of Macromolecular Assemblies. New York: Oxford University Press. ... Journal of Microscopy. 127 (2): 127-138. doi:10.1111/j.1365-2818.1982.tb00405.x. PMID 7120365. S2CID 27206060. Böttcher, B.; ... "Determination of the fold of the core protein of hepatitis B virus by electron microscopy". Nature. 386 (6620): 88-91. doi: ...
https://en.wikipedia.org/wiki/Spectral_signal-to-noise_ratio
RasMolRasMol
... or cryo-electron microscopy. Rasmol can communicate with other programs via Tcl/Tk on Unix platforms, and via Dynamic Data ...
https://en.wikipedia.org/wiki/RasMol
Electron tomographyElectron tomography
In the field of biology, bright-field transmission electron microscopy (BF-TEM) and high-resolution TEM (HRTEM) are the primary ... Tomography Tomographic reconstruction Cryo-electron tomography Positron emission tomography Crowther criterion X-ray computed ... Midgley, P. A.; Weyland, M. (2003). "3D electron microscopy in the physical sciences: The development of Z-contrast and EFTEM ... Bals, S.; Kisielowski, C. F.; Croitoru, M.; Tendeloo, G. V. (2005). "Annular Dark Field Tomography in TEM". Microscopy and ...
https://en.wikipedia.org/wiki/Electron_tomography
Kinesin-like protein KIF11Kinesin-like protein KIF11
X-ray crystallography, cryo-electron microscopy, and real-time infrared spectroscopy have been used to measure the structure of ...
https://en.wikipedia.org/wiki/Kinesin-like_protein_KIF11
List of German inventors and discoverersList of German inventors and discoverers
Shared the Nobel Prize for Medicine 1956 Joachim Frank: co-invented cryo-electron microscopy. Shared the Nobel Prize for ... 600 patents in fields including television and radio, electron microscopy, medical technology, nuclear technology, and plasma ...
https://en.wikipedia.org/wiki/List_of_German_inventors_and_discoverers
Mark SchlisselMark Schlissel
... cryo-electron microscopy and RNA-based therapeutics, in addition to several exploratory grants for earlier-phase research. As ...
https://en.wikipedia.org/wiki/Mark_Schlissel
Vault (organelle)Vault (organelle)
They measure 34 nm by 60 nm from a negative stain, 26 nm by 49 nm from cryo-electron microscopy, and 35 nm by 59 nm from STEM. ...
https://en.wikipedia.org/wiki/Vault_(organelle)
NucleoproteinNucleoprotein
... nuclear magnetic resonance and cryo-electron microscopy. Virus genomes (either DNA or RNA) are extremely tightly packed into ...
https://en.wikipedia.org/wiki/Nucleoprotein
BsoftBsoft
Heymann JB, Cardone G, Winkler DC and Steven AC (2008) Computational resources for cryo-electron tomography in Bsoft. Journal ... EM Data Bank Bsoft home page Software Tools For Molecular Microscopy (Articles with short description, Short description is ... Heymann JB and Belnap DM (2007) Bsoft: Image processing and molecular modeling for electron microscopy. Journal of Structural ... Image and molecular processing in electron microscopy. Journal of Structural Biology 133(2/3), 156 - 169. ...
https://en.wikipedia.org/wiki/Bsoft
Valosin-containing proteinValosin-containing protein
"Motions and negative cooperativity between p97 domains revealed by cryo-electron microscopy and quantised elastic deformational ...
https://en.wikipedia.org/wiki/Valosin-containing_protein
Resolution (electron density)Resolution (electron density)
In cryo-electron microscopy, resolution is typically measured by the Fourier shell correlation (FSC), a three-dimensional ... while resolution in cryo-electron microscopy is a frequency space comparison of two halves of the data, which strives to ... Frank, Joachim (2006). Three-Dimnsional Electron Microscopy of Macromolecular Assemblies. New York: Oxford University Press. ... Journal of Microscopy. 127 (2): 127-138. doi:10.1111/j.1365-2818.1982.tb00405.x. PMID 7120365. S2CID 27206060. Böttcher, B.; ...
https://en.wikipedia.org/wiki/Resolution_(electron_density)
Centrosome cycleCentrosome cycle
Chrétien D, Buendia B, Fuller SD, Karsenti E (November 1997). "Reconstruction of the centrosome cycle from cryoelectron ... "Centriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy". The Journal of Cell Biology. ...
https://en.wikipedia.org/wiki/Centrosome_cycle
2000s2000s
RNA interference Neutrino insights Genome progress CMB structure and polarization Attosecond physics TRP channels Cryoelectron ... fossil fish Cloaking technology Macular degeneration patients Biodiversity and speciation Sub-diffraction-limit microscopy LTP ...
https://en.wikipedia.org/wiki/2000s
MetabolonMetabolon
... and was observed using chemical cross-linking coupled to mass spectrometry and visualized by cryo-electron microscopy. The ...
https://en.wikipedia.org/wiki/Metabolon
Biological data visualizationBiological data visualization
... visualization of larger complexes by cryo-electron microscopy, and visualization of the location of proteins and complexes ... Plattner H, Hentschel J (2006). "Sub-Second Cellular Dynamics: Time-Resolved Electron Microscopy and Functional Correlation". A ... Genomic and assembly data Alignments, phylogeny and evolution Systems biology Molecular graphics Microscopy Magnetic resonance ... electron microscopy and cell and tissue imaging. In contrast, visualization of trajectories has long been a prominent part of ...
https://en.wikipedia.org/wiki/Biological_data_visualization
PresenilinPresenilin
Structures have been solved of the assembled gamma secretase complex by cryo-electron microscopy, demonstrating significant ... as well as cryo-electron microscopy of the human gamma-secretase complex, indicate nine transmembrane helices. Presenilins are ...
https://en.wikipedia.org/wiki/Presenilin
Shlomo RakibShlomo Rakib
"Representation theoretic patterns in three dimensional cryo-electron microscopy III - Presence of Point Symmetries." Gurevich, ...
https://en.wikipedia.org/wiki/Shlomo_Rakib
PyrenoidPyrenoid
When examined with transmission electron microscopy, the pyrenoid matrix appears as a roughly circular electron dense granular ... "Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography". eLife. 4: e04889. doi: ... visible using light microscopy. By contrast, in diatoms and dinoflagellates, there can be multiple pyrenoids. The Chlamydomonas ...
https://en.wikipedia.org/wiki/Pyrenoid
PSMD1PSMD1
... provides an alternative structure of 19S base via an integrative approach combining data from cryoelectron microscopy, X-ray ...
https://en.wikipedia.org/wiki/PSMD1
Transformative High-Resolution Cryoelectron Microscopy (CryoEM)Transformative High-Resolution Cryoelectron Microscopy (CryoEM)
The program aims to broaden access to high-resolution cryoelectron microscopy (cryoEM) for biomedical researchers, by creating ... Transformative High Resolution Cryo-Electron Microscopy (CryoEM). *Transformative Research to Address Health Disparities and ... Transformative High-Resolution Cryoelectron Microscopy Program. The program is broadening access to high-resolution ... Putting the freeze on COVID-19: Collecting images of SARS-CoV-2 using cryoelectron microscopy. Read highlight here! ...
https://commonfund.nih.gov/cryoem
The integrative role of cryo electron microscopy in molecular and cellular structural biologyThe integrative role of cryo electron microscopy in molecular and cellular structural biology
... the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of ... Keywords: Crystallography; Structural biology; Super-resolution microscopy; cryo electron microscopy; cryo electron tomography ... The integrative role of cryo electron microscopy in molecular and cellular structural biology Biol Cell. 2017 Feb;109(2):81-93. ... the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of ...
https://pubmed.ncbi.nlm.nih.gov/27730650/
Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image...Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image...
Using a synergistic application of cryo-electron microscopy, cryo-electron tomography, and three-dimensional reconstruction, we ... Kokkinopoulou et al.9, for example, applied transmission electron microscopy (TEM) and cryo-electron microscopy (cryo-EM) to ... Using a synergistic application of cryo-electron microscopy, cryo-electron tomography, and three-dimensional reconstruction, we ... Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image ...
https://www.nature.com/articles/s41467-020-20884-9?error=cookies_not_supported&code=e2b1be5b-5800-48d1-b51a-bd8b103b8b09
Cryo-Electron Microscopy - EMBL Imaging Centre
The Cryo-Electron Microscopy Service Platform at EMBL Heidelberg is now offering remote service to all interested users, ... EMBL Heidelberg hosts a cryo-electron microscopy (cryo-EM) service platform, available for use by external scientists with both ... Open access to cutting-edge electron and light microscopy We provide researchers from Europe and beyond with a synergistic ... including the COVID-19-related projects (single-particle analysis and cryo-electron tomography). Due to the current situation, ...
https://www.embl.org/about/info/imaging-centre/cryo-electron-microscopy/
Cryo-electron Microscopy Offers Atomic-Level Images of How Drug Binds to Cancer ProteinCryo-electron Microscopy Offers Atomic-Level Images of How Drug Binds to Cancer Protein
Researchers have produced atomic-level cryo-electron microscopy (cryo-EM) images detailing how a small molecule allosteric- ... Cryo-electron Microscopy Offers Atomic-Level Images of How Drug Binds to Cancer Protein. ... Researchers have produced atomic-level cryo-electron microscopy (cryo-EM) images detailing how a small molecule allosteric- ... Researchers have produced atomic-level cryo-electron microscopy (cryo-EM) images detailing how a small molecule allosteric- ...
https://www.cancernetwork.com/view/cryo-electron-microscopy-offers-atomic-level-images-how-drug-binds-cancer-protein
AlbaNova | Computational Reconstruction in Cryo-Electron MicroscopyAlbaNova | Computational Reconstruction in Cryo-Electron Microscopy
... and extract the inherent molecular flexibility and motions from the cryo-electron micrographs. Finally, I will describe some ...
https://www.albanova.se/event/computational-reconstruction-in-cryo-electron-microscopy/
Novel AI-Based Method to Analyze Cryo-Electron Microscopy DataNovel AI-Based Method to Analyze Cryo-Electron Microscopy Data
Cryo-electron microscopy is a fairly new method used to examine the structure of materials, proteins or cells. Theoretically, ... But, cryo-electron microscopy offers only three-dimensional structures, and it cannot identify proteins to categorize their ... Novel AI-Based Method to Analyze Cryo-Electron Microscopy Data. *Download PDF Copy ... The researchers efficiently developed an AI-based approach for analyzing data from cryo-electron microscopy. In the coming days ...
https://www.azorobotics.com/News.aspx?newsID=12750
K2 Summit camera enables scientists to break the 3 Å barrier in structural biology using cryo-electron microscopy (cryo-EM) |...K2 Summit camera enables scientists to break the 3 Å barrier in structural biology using cryo-electron microscopy (cryo-EM) |...
... high-resolution structures solved by single particle cryo-electron microscopy (cryo-EM) created speculation that 3 Å might be ... K2 Summit camera enables scientists to break the 3 Å barrier in structural biology using cryo-electron microscopy (cryo-EM). ... For many years the lack of sub 3 Å (angstrom) high-resolution structures solved by single particle cryo-electron microscopy ( ... Å resolution reconstruction of the Thermoplasma acidophilum 20 S proteasome using cryo electron microscopy, eLife 2015;10.7554/ ...
https://www.gatan.com/company/news/k2-summit-camera-enables-scientists-break-3-%C3%A5-barrier-structural-biology-using-cryo
Cryo-electron microscopy reveals detailed blueprint of viral genome replication machinery - Master DoctorCryo-electron microscopy reveals detailed blueprint of viral genome replication machinery - Master Doctor
Cryo-electron microscopy reveals detailed blueprint of viral genome replication machinery 13 days ago - By News Medical ...
http://www.masterdoctor.net/news/cryo-electron-microscopy-reveals-detailed-blueprint-of-viral-genome-replication-machinery.n/?channel=
Frontiers | Discovery of Exosomes From Tick Saliva and Salivary Glands Reveals Therapeutic Roles for CXCL12 and IL-8 in Wound...Frontiers | Discovery of Exosomes From Tick Saliva and Salivary Glands Reveals Therapeutic Roles for CXCL12 and IL-8 in Wound...
Cryo-electron microscopy (cryo-EM) analysis revealed that tick saliva and salivary glands are composed of heterogeneous ... Cryo-Electron Microscopy. Cryo-electron microscopy (cryo-EM) was performed at two independent institutional facilities [ ... Cryo-Electron Microscopy Analysis Revealed a Novel Discovery of Exosomes in Tick Saliva and Salivary Glands. In this study, we ... A) Cryo-electron microscopy (Cryo-EM) images collected from two independent institutions [University of Texas Medical Branch ( ...
https://www.frontiersin.org/articles/10.3389/fcell.2020.00554/full
Plasmodium yoelii: influence of immune modulators on the development of the liver stagePlasmodium yoelii: influence of immune modulators on the development of the liver stage
Cryoelectron Microscopy * Cyclophosphamide / pharmacology * Dexamethasone / pharmacology * Female * Glucocorticoids / ...
https://pubmed.ncbi.nlm.nih.gov/20493849/?dopt=Abstract
Microscopic images and protein [IMAGE] | EurekAlert! Science News ReleasesMicroscopic images and protein [IMAGE] | EurekAlert! Science News Releases
Research methods/Microscopy/Cryo electron microscopy * /Research methods/Microscopy/Electron microscopy/Transmission electron ... A new tool for cryo-electron microscopy. Forschungszentrum Juelich. Journal. Nature Methods. DOI. 10.1038/s41592-022-01586-0. ... www.fz-juelich.de/en/news/archive/notifications/2022/a-new-tool-for-cryo-electron-microscopy ...
https://www.eurekalert.org/multimedia/948751
Cryoelectron microscopy of mouse mammary tumor virus :: MPG.PuReCryoelectron microscopy of mouse mammary tumor virus :: MPG.PuRe
Briggs, J. A. G., Watson, B. E., Gowen, B. E., & Fuller, S. D. (2004). Cryoelectron microscopy of mouse mammary tumor virus. ... Cryoelectron microscopy of Mouse mammary tumor virus, a Betaretrovirus, provided information about glycoprotein structure and ... Cryoelectron microscopy of mouse mammary tumor virus ...
https://pure.mpg.de/pubman/faces/ViewItemOverviewPage.jsp?itemId=item_3348934
Helmholtz Imaging Modalities - Related facilities to Cryo Electron Microscopy
related facilities to Cryo Electron Microscopy. Sorry, no facilities related to Cryo Electron Microscopy. ...
https://modalities.helmholtz-imaging.de/related/modality/123/facilities
Cryo-Electron Microscopy Study of Vesicles from Various Species - RICCEMCryo-Electron Microscopy Study of Vesicles from Various Species - RICCEM
Cryo-Electron Microscopy Study of Vesicles from Various Species Evgeny V. Yastremsky, Luiza A. Garaeva, Elena D. Putevich, ...
https://riccem.org/yastremsky-2/
Advanced Center For Cryo-Electron Microscopy Facility - IISc - Bangalore India.
The Advanced Center for Cryo-Electron Microscopy Facility (ACCEM-IISc) at IISc Bengaluru is a world class cryo-EM facility in ... Copyright: Advanced Center For Cryo-Electron Microscopy Facility - IISc Bangalore. Powered by Surfzone Technologies ... scientists all over the country to implement single particle cryo-EM and cryo-electron tomography (cryo-ET) for high-resolution ...
http://mbu.iisc.ac.in/SDLab.htm/index.php
Muscle cells need calcium ions | Max-Planck-GesellschaftMuscle cells need calcium ions | Max-Planck-Gesellschaft
... developed by Max Planck researchers in Göttingen visualizes individual atoms in a protein with cryo electron microscopy for the ... In the current research the vesicles of sarcoplasmic reticulum were purified, imaged by cryo-electron tomography and the ...
https://www.mpg.de/14590554/muscle-cells-need-calcium-ions
Cryo electron microscopy of SARS-CoV-2 spike in prefusion state
Hyderabad: Cryo- electron microscopy ushered in at CSIR-CCMB - Telangana TribuneHyderabad: Cryo- electron microscopy ushered in at CSIR-CCMB - Telangana Tribune
Cryo- electron microscopy ushered in at CSIR-CCMB. Hyderabad: An innovative center for cryo-electron microscopy was ushered in ... Hyderabad: Cryo- electron microscopy ushered in at CSIR-CCMB. by Telangana Tribune March 25, 2022 24 0 Comment 3 min read ... "The modern cryo-electron microscopy facility is expected to help us view the functioning of several molecular machines that ... Hyderabad is the 2nd city in the country to organize a contemporary cryo-electron microscopy center. Such a center enables ...
https://www.telanganatribune.com/hyderabad/hyderabad-cryo-electron-microscopy-ushered-in-at-csir-ccmb/
Items where Subject is Cryo-electron microscopy - CSHL Scientific Digital RepositoryItems where Subject is "Cryo-electron microscopy" - CSHL Scientific Digital Repository
Items where Subject is "Cryo-electron microscopy" Up a level. Export as ASCII Citation. BibTeX. Check URLs. Dublin Core. EP3 ...
http://repository.cshl.edu/view/subjects/Cryo=5Felectron=5Fmicroscopy.html
A national facility for biological cryo-electron microscopy. - CAMS Oxford Institute
Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated ... Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an ... three-dimensional electron microscopy, Academies and Institutes, Automation, Cryoelectron Microscopy, Crystallography, United ... Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an ...
https://www.camsoxford.ox.ac.uk/coi-publications/publications/505154
Research News: U-Wisconsin Adding Cryo-Electron Microscopy to Imaging Arsenal
How cryo-electron microscopy differs from other imaging techniques?. Cryo-EM gives researchers an extremely detailed view of a ... Research News: U-Wisconsin Adding Cryo-Electron Microscopy to Imaging Arsenal Posted by Rebecca Inch-Partridge on Tue, Feb 13, ... Now UW-Madison is adding another piece of equipment to their state-of-the-art imaging arsenal: a cryo-electron microscopy (cryo ... The BOC provides state-of-the-art instrumentation for light and fluorescence based microscopy, including epifluorescence, ...
https://info.biotech-calendar.com/research-news-u-wisconsin-adding-cryo-electron-microscopy-to-imaging-arsenal
Omicron's Spike Protein Captured via Cryo-Electron Microscopy | Natural healing and nutrition
Omicrons Spike Protein Captured via Cryo-Electron Microscopy. by V Rao , Jan 24, 2022 , news , 0 comments ... The near-atomic resolution analysis-using cryo-electron microscopy-yields insights into how the heavily mutated Omicron variant ...
https://sciencelock.in/omicrons-spike-protein-captured-via-cryo-electron-microscopy/
Researchers use cryo-electron microscopy to resolve 3D structure of protein nanoparticleResearchers use cryo-electron microscopy to resolve 3D structure of protein nanoparticle
... *Download PDF Copy ... Tags: Compound, Drug Delivery, E. coli, Electron, Electron Microscopy, Helix, Microscopy, Molecule, Nanoparticle, Nanoparticles ... A large amount of TIP60 was expressed in E. coli, and a purified sample was observed at the cryo-electron microscope facility ... research group solved the detailed three-dimensional structure of the TIP60 using single-particle cryo-electron microscopy. ...
https://www.azolifesciences.com/news/20211006/Researchers-use-cryo-electron-microscopy-to-resolve-3D-structure-of-protein-nanoparticle.aspx
Multipartners communication between organelles deciphered by cryo-electron microscopy Daniel Levy PCC ParisMultipartners communication between organelles deciphered by cryo-electron microscopy Daniel Levy PCC Paris
Multipartners communication between organelles deciphered by cryo-electron microscopy Doc envoyé stage_M2-Dlevy-Curie-MCS.pdf ... In vitro and in cellulo characterization of VapB-Microtubules interaction by Cryo Electron Microscopy and optical microscopies ... Multipartners communication between organelles deciphered by cryo-electron microscopy _ Daniel Levy _ PCC _ Paris. Home / stage ... 3D architecture at sub-nanometric resolution of BLOC-1 endosomal complex by cryo-electron microscopy _ Daniel Levy _ PCC _ ...
https://master-physique-biologie.fr/multipartners-communication-between-organelles-deciphered-by-cryo-electron-microscopy/
SARS-CoV-2 neutralizing antibody structures inform therapeutic strategies - CaltechAUTHORSSARS-CoV-2 neutralizing antibody structures inform therapeutic strategies - CaltechAUTHORS
Antibodies; Cryoelectron microscopy; SARS-CoV-2. Issue or Number:. 7839. PubMed Central ID:. PMC8092461. ... Electron microscopy was performed in the Caltech Beckman Institute Resource Center for Transmission Electron Microscopy with ... C135-S 2P and C144-S 6P complexes have been deposited at the PDB and the Electron Microscopy Data Bank (EMDB) under the ...
https://authors.library.caltech.edu/105592/
Resolving the Morphology of Peptoid Vesicles at the 1 nm Length Scale Using Cryogenic Electron Microscopy (Journal Article) |...Resolving the Morphology of Peptoid Vesicles at the 1 nm Length Scale Using Cryogenic Electron Microscopy (Journal Article) |...
Cryo-electron microscopy of artificial biological membranes journal, November 1985 * Lepault, Jean; Pattus, Franc; Martin, ... MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy journal, February 2017 * Zheng ... A new method to measure bilayer thickness: Cryo-electron microscopy of frozen hydrated liposomes and image simulation journal, ... Electron microscopy of the stacked disk aggregate of tobacco mosaic virus protein journal, August 1974 * Unwin, P. N. T.; Klug ...
https://www.osti.gov/pages/biblio/1492105-img68604-table
Drug Resistance - GEN - Genetic Engineering and Biotechnology NewsDrug Resistance - GEN - Genetic Engineering and Biotechnology News
Therapeutic Target against Common Fungus Uncovered by Cryo-Electron Microscopy. Drug Resistance Phage Used to Clear Antibiotic- ...
https://www.genengnews.com/category/drug-resistance/
Cryoelectron-microscopy framework of the enteropathogenic Escherichia coli variety Three secretion method EspA filament.Cryoelectron-microscopy framework of the enteropathogenic Escherichia coli variety Three secretion method EspA filament.
ElectronCrystallographyTomographySpectroscopyResearchersCharacterizationCentersConfocalFluorescenceEscherichiaReconstructionEnablesStructureResearchScientistsTransmission electron mCRYOEMSingle-particle cryo-electron m2020ProteinsCorrelativeCryogenicMacromolecularStructuresFacilityCryo-electron microscoTechnique called cryo-electron mLight microscopyReconstructionsProteinStructuralBiomoleculesHelicalLife sciencesAtomic resolutionComputationalImageHigh-resolutionReceptor2017BiochemistryElectronsDataRibosomeResolutionMoleculesAdvancesNegative stainPreparation
Electron56
  • After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various sizes. (nih.gov)
  • Using a synergistic application of cryo-electron microscopy, cryo-electron tomography, and three-dimensional reconstruction, we revealed the unique morphological details of the biomolecules and their distribution/association with the surface of polystyrene NPs at a nanoscale resolution. (nature.com)
  • Other techniques to characterize the BC, such as gel electrophoresis, differential centrifugal sedimentation, and conventional electron microscopy, also fail to provide structural details. (nature.com)
  • The objective of the current study is to investigate the morphological details of the biomolecules and their distribution and association with the surface of carboxylated polystyrene NPs (PS-COOH NPs) at the nanometer scale using high-resolution transmission electron microscopy (HRTEM), cryo-EM, cryo-electron tomography (cryo-ET), and image analysis. (nature.com)
  • EMBL Heidelberg hosts a cryo-electron microscopy (cryo-EM) service platform, available for use by external scientists with both single particle and tomography projects. (embl.org)
  • The Cryo-Electron Microscopy Service Platform at EMBL Heidelberg is now offering remote service to all interested users, including the COVID-19-related projects (single-particle analysis and cryo-electron tomography). (embl.org)
  • Researchers have produced atomic-level cryo-electron microscopy (cryo-EM) images detailing how a small molecule allosteric-inhibiting drug binds to p97, a cancer cell protein, they reported in the journal Science. (cancernetwork.com)
  • The researchers efficiently developed an AI-based approach for analyzing data from cryo-electron microscopy. (azorobotics.com)
  • Cryo-electron microscopy is a fairly new method used to examine the structure of materials, proteins or cells. (azorobotics.com)
  • But, cryo-electron microscopy offers only three-dimensional structures, and it cannot identify proteins to categorize their identity. (azorobotics.com)
  • For many years the lack of sub 3 Å (angstrom) high-resolution structures solved by single particle cryo-electron microscopy (cryo-EM) created speculation that 3 Å might be the fundamental resolution limit of the technique. (gatan.com)
  • The research article 2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20 S proteasome using cryo electron microscopy , eLife 2015;10.7554/eLife.06380, demonstrates that with the help of the K2 Summit camera, single-particle cryo-EM can compete with x-ray crystallography to determine protein structures for rational drug design at 2.8 Å resolution. (gatan.com)
  • Cryo-electron microscopy (cryo-EM) analysis revealed that tick saliva and salivary glands are composed of heterogeneous populations of in vivo exosomes with sizes ranging from 30 to 200 nm. (frontiersin.org)
  • The Advanced Center for Cryo-Electron Microscopy Facility (ACCEM-IISc) at IISc Bengaluru is a world class cryo-EM facility in India, which provides access to the researchers & scientists all over the country to implement single particle cryo-EM and cryo-electron tomography (cryo-ET) for high-resolution imaging and structural characterization of biological macromolecules. (iisc.ac.in)
  • An innovative center for cryo-electron microscopy was ushered in by Dr Shekhar Mande, Director-General, Council of Scientific and also Industrial Research (CSIR), at the Centre for Cellular and also Molecular Biology (CCMB) on Friday. (telanganatribune.com)
  • A launch claimed, with this center, Hyderabad is the 2nd city in the country to organize a contemporary cryo-electron microscopy center. (telanganatribune.com)
  • The modern cryo-electron microscopy facility is expected to help us view the functioning of several molecular machines that operate in the cell that was earlier not amenable to conventional structure determination methods such as X-ray crystallography or Nuclear Magnetic Resonance (NMR)," claimed Dr Rajan Sankaranarayanan, a noteworthy architectural biologist at CCMB. (telanganatribune.com)
  • The chasm between structural and cellular biology is diminishing, and this will allow addressing some of the very fundamental and exciting problems of biology with techniques like cryo-electron microscopy," claimed Dr Shekhar Mande, Director-General, CSIR. (telanganatribune.com)
  • A national facility for biological cryo-electron microscopy. (ox.ac.uk)
  • Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. (ox.ac.uk)
  • Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. (ox.ac.uk)
  • Now UW-Madison is adding another piece of equipment to their state-of-the-art imaging arsenal: a cryo-electron microscopy (cryo-EM) facility. (biotech-calendar.com)
  • How cryo-electron microscopy differs from other imaging techniques? (biotech-calendar.com)
  • The near-atomic resolution analysis-using cryo-electron microscopy-yields insights into how the heavily mutated Omicron variant attaches to and infects human cells. (sciencelock.in)
  • In the present study, the joint research group solved the detailed three-dimensional structure of the TIP60 using single-particle cryo-electron microscopy. (azolifesciences.com)
  • In vitro and in cellulo characterization of VapB-Microtubules interaction by Cryo Electron Microscopy and optical microscopies. (master-physique-biologie.fr)
  • A new approach for characterizing the vesicle membrane morphology based on low-dose cryogenic electron microscopy (cryo-EM) is described. (osti.gov)
  • Candidates using cryo-electron microscopy techniques to explore cutting-edge questions of structure and function are encouraged to apply. (cell.com)
  • Structural transitions in the GTP cap visualized by cryo-electron microscopy of catalytically inactive microtubules. (icr.ac.uk)
  • Here, we use cryo-electron microscopy and total internal reflection fluorescence microscopy of GTP hydrolysis-deficient MTs assembled from mutant recombinant human tubulin to investigate the structure of a GTP-bound MT lattice. (icr.ac.uk)
  • In situ transmission electron microscopy (TEM) is one of the most powerful approaches for revealing physical and chemical process dynamics at atomic resolutions. (semanticscholar.org)
  • Direct Observation of Oxygen Evolution and Surface Restructuring on Mn2O3 Nanocatalysts Using In Situ and Ex Situ Transmission Electron Microscopy. (semanticscholar.org)
  • Transmission electron microscopy (TEM) has long been used as the ultimate characterization technique for nanomaterials at the atomic level. (semanticscholar.org)
  • Using cryogenic-electron microscopy, we present a model of RyR1 with a 2.45-Å resolution before local refinement, revealing a binding site in the RY1&2 domain (3.10 Å local resolution), where the Rycal ARM210 binds cooperatively with ATP and stabilizes the closed state of RyR1. (cell.com)
  • ATP-Bound States of Groel Captured by Cryo-Electron Microscopy. (expasy.org)
  • It was obtained through cryo-electron microscopy. (scienceblogs.com)
  • The resolution isn't great, because electron microscopy can only do so much, so the structure model is built from the alpha carbon backbones of these 420 proteins. (scienceblogs.com)
  • Analytical Cryo-Scanning Electron Microscopy of Hydrated Polymers and Microgels. (stevens.edu)
  • Jason has recently joined the Industrial Liaison Office (May 2017) as a cryo-electron microscopy scientist where he will be supporting industry clients seeking advanced imaging capabilities of eBIC. (diamond.ac.uk)
  • for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution. (nobelprize.org)
  • It provides users with access to a powerful and synergistic portfolio in light and electron microscopy technologies and correlative approaches, including academically developed methods not yet commercially available. (embl.org)
  • Users showed particularly strong interest in cryo-electron and super-resolution microscopy technologies, with samples covering different mammalian species, but also plants, bacteria and marine organisms," she said. (embl.org)
  • An international team of researchers at OIST and University of Otago have used the Nobel-winning cryo-electron microscopy method to reconstruct t. (oist.jp)
  • Using a combination of femtosecond spectroscopy with electron microscopy techniques, the flow of electrons on femtosecond timescales was tracked. (oist.jp)
  • The structures were created using the cryo-electron microscopy method, by freezing infectious BKV particles and taking thousands of images using the microscopes. (wordpress.com)
  • Professor Neil Ranson, Director of Cryo-Electron Microscopy at the University of Leeds, said: "Cryo-electron microscopy has been around for 30 years and has been incredibly useful, but until recently the technology lacked the ability to routinely look at molecules at the level of detail needed. (wordpress.com)
  • We used newly developed techniques for semiautomated reconstruction from serial electron microscopy (EM) [14] to obtain the shapes of neurons, the location of synapses, and the resultant synaptic connectivity. (janelia.org)
  • The challenge of recovering the topology of massive neuronal circuits can potentially be met by high throughput Electron Microscopy (EM) imagery. (janelia.org)
  • Semi-automated reconstruction of neural circuits using electron microscopy. (janelia.org)
  • Previously used to reconstruct the C. elegans wiring diagram, serial-section transmission electron microscopy (ssTEM) is a proven technique for the task. (janelia.org)
  • We review progress in the processing of electron microscopy images and, in particular, a semi-automated reconstruction pipeline deployed at Janelia. (janelia.org)
  • Increasing depth resolution of electron microscopy of neural circuits using sparse tomographic reconstruction. (janelia.org)
  • Jan. 10, 2020-D. Borden Lacy and colleagues used cryo-electron microscopy to define the structure of a C. diff toxin, providing a framework for the design of novel therapeutics. (vumc.org)
  • Vicki is visiting us from the newly established Living Systems Institute (LSI) Exeter, where she and her group research protein transport across membranes, with a strong methodological focus on cryo-electron microscopy. (cam.ac.uk)
  • JEOL USA awarded two Grand Prizes to winners of its 2020 Electron Microscopy Image Contest, and kicked off its 2021 Image Contest at the beginning of the new year. (labbulletin.com)
  • Lita explains that "Manipulating Drosophila melanogaster is a bit challenging from an electron microscopy point of view, but is so indispensable for genomic research in Amyotrophic lateral sclerosis (ALS), Alzheimer's, and other debilitating diseases. (labbulletin.com)
Crystallography1
  • Cryoelectron microscopy and x-ray crystallography analyses of NoV VLPs identified the shell (S) and protruding domains (subdomains P1-1, P1-2, and P2) ( 18 ). (cdc.gov)
Tomography1
  • The program is broadening access to high-resolution cryoelectron microscopy (cryoEM) and tomography (cryoET) for biomedical researchers by creating national service centers, and cultivating a skilled workforce through the development and implementation of cryoEM tra ining material . (nih.gov)
Spectroscopy1
  • This, along with the confocal microscopy, NMR spectroscopy and also X-ray diffraction centers at CCMB, makes it an awesome center for scientists to consider information of living cells like never ever previously. (telanganatribune.com)
Researchers2
  • We provide researchers from Europe and beyond with a synergistic portfolio of imaging services including cryo-EM, super-resolution and intravital microscopy to enable new ground-breaking research that crosses the scales of biology. (embl.org)
  • To offer researchers from academia and industry rapid access to the most advanced microscopy technologies available, in recent months the EMBL Imaging Centre staff have undertaken testing and equipment commissioning including internal and external user trials. (embl.org)
Characterization1
  • Microscopy 101: Scanning probes or scanning electrons: a practical guide to select a method for nanoscale characterization. (stevens.edu)
Centers1
  • Did you miss one of the free monthly webinars on cryoEM current practices and strategies hosted by the National Centers for Cryoelectron Microscopy? (nih.gov)
Confocal1
  • The BOC provides state-of-the-art instrumentation for light and fluorescence based microscopy, including epifluorescence, confocal, and super-resolution imaging. (biotech-calendar.com)
Fluorescence1
  • Single molecule fluorescence microscopy has the unique advantage to provide real-time information on the spatiotemporal assembly of individual protein complexes in cellular membranes. (bvsalud.org)
Escherichia1
  • Cryoelectron-microscopy framework of the enteropathogenic Escherichia coli variety Three secretion method EspA filament. (socialmediastore.net)
Reconstruction1
  • Finally, I will describe some very recent work where we try to combine experimental and theoretical methods to study ensembles of molecular conformations, and how we would like to use these types of reconstruction methods for wider classes of experimental data, including e.g. neutron scattering and light microscopy. (albanova.se)
Enables1
  • For proteins, the microscopy enables highly elaborate images of the structure to be created at the level of single-atom. (azorobotics.com)
Structure1
  • Cryoelectron microscopy of Mouse mammary tumor virus, a Betaretrovirus, provided information about glycoprotein structure and core formation. (mpg.de)
Research1
  • A form of microscopy is shaking up nanoscience research and forcing scientists to reconsider many established theories. (chemistryworld.com)
Scientists1
  • Microscopy is an important tool for scientists in the study of cells, tissues, and organs. (nih.gov)
Transmission electron m8
  • The calculated structures are cross-validated using an independent data set of 691 ssNMR constraints and scanning transmission electron microscopy measurements. (mpg.de)
  • Transmission electron microscopy and cryo electron microscopy analysis of standard myelinosome preparation from TM4 Sertoli cells and human seminal plasma samples. (archives-ouvertes.fr)
  • The technological advances of the 1940s allowed the observation of cellular organelles embedded in heavy metal stains by transmission electron microscopy (TEM). (nih.gov)
  • Transmission electron microscopy (TEM) is an essential method in virology because it allows for direct visualization of virus morphology at a nanometer scale. (nature.com)
  • Observing viral morphology is essential in virology, for which transmission electron microscopy (TEM) is the most widely used technique because it allows direct visualization at the nanometer scale. (nature.com)
  • d ) Transmission electron microscopy (TEM) of heavy-metal-coated virions produces ( e ) a reverse-contrast image of the virus. (nature.com)
  • Mineralization in the MVs will be visualized by cryo-transmission electron microscopy with a temporal resolution to decipher the pathway by which mineral is formed in the MVs. (fapesp.br)
  • Volta potential phase plate for in-focus phase contrast transmission electron microscopy. (mpg.de)
CRYOEM11
  • The Cryo-Electron Microscopy (CRYOEM) core provides the NIDDK scientific community with equipment and training in preparing and imaging negative stain and cryo-EM grids. (nih.gov)
  • The program is broadening access to high-resolution cryoelectron microscopy (cryoEM) and tomography (cryoET) for biomedical researchers by creating national service centers, and cultivating a skilled workforce through the development and implementation of cryoEM tra ining material . (nih.gov)
  • Did you miss one of the free monthly webinars on cryoEM current practices and strategies hosted by the National Centers for Cryoelectron Microscopy? (nih.gov)
  • This NIH Common Fund initiative will establish national service centers to increase research capacity for molecular structure determination by high resolution cryoelectron microscopy (cryoEM). (nih.gov)
  • Cryoelectron microscopy (cryoEM) has been selected for Common Fund support because it opens up previously inaccessible levels of biological organization to high resolution structure analysis, and will vastly extend the range of functions and interactions that can be targeted for basic research and structure-based development of therapeutics. (nih.gov)
  • An emerging related cryoEM method, cryoelectron tomography (cryoET), can image macromolecular assemblies in intact unstained frozen cells in 3-D. Together, these methods enable high-resolution analysis of previously inaccessible levels of biological organization, ranging from below 100kD to whole cells. (nih.gov)
  • A. The purpose of this funding opportunity announcement (FOA) is to provide regional access to state-of-the-art data collection capabilities to cryoelectron microscopy (cryoEM) laboratories. (nih.gov)
  • Development of cryogenic electron microscopy (cryoEM) in the 1970s provided the means to image these macromolecules while keeping them hydrated in biochemically functional buffer conditions by instantaneously freezing them at −180°C. However, the resolution attainable for these samples through cryoEM was limited. (nih.gov)
  • The lab uses biochemistry, cryo electron microscopy (cryoEM), and other tools to attack the problems, while also working on method development to expand applications of cryoEM on life sciences research. (nih.gov)
  • We use cryo electron microscopy (cryoEM) as a primary tool to study the structures of membrane proteins. (nih.gov)
  • Given these challenges, we envision that application of the recently reported cryo-electron microscopy (CryoEM) modality micro-crystal electron diffraction (MicroED) could lead to vertical advances in the field of NP discovery directly responsive to this NOSI, as MicroED has recently been demonstrated to provide unambiguous structures from sub-micron-sized crystals of structurally complex chemical compounds that had failed to yield large crystals suitable for X-ray analysis. (nih.gov)
Single-particle cryo-electron m3
  • To further investigate the relationships between BtAdVs and CAdVs, we conducted mass spectrometric analysis and single-particle cryo-electron microscopy reconstructions of the BtAdV 250-A capsid and also analyzed the in vitro host ranges of both viruses. (elsevier.com)
  • Together, these results provide insights into the reductive nature of microsporidian evolution and unravel a novel mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. (imex-revista.com)
  • The conserved theme of ribosome dimerization revealed by single-particle cryo-electron microscopy. (imex-revista.com)
20201
  • 2020) Cryo-Electron Microscopy of Endogenous Yeast Exosomes. (cls.edu.cn)
Proteins9
  • Electron microscopy, in ever-more-incremental strokes, has revealed this world - without it, we would not be able to see viruses or proteins, keys to understanding the processes of life. (freethink.com)
  • IRP researchers led by Sriram Subramaniam, Ph.D. , developed groundbreaking new technology based on cryo-electron microscopy (cryo-EM) to determine the atomic structures of proteins. (nih.gov)
  • NIH funded the development and dissemination of cryo-electron microscopy (cryo-EM), a tool that enables high-resolution images of proteins and other biological structures. (nih.gov)
  • Using cryo-electron microscopy, they have uncovered the mechanisms by which some HAT proteins transport certain amino acids and not others. (cnio.es)
  • Thanks to the latest high-resolution structural technologies such as cryo-electron microscopy, combined with computational modelling and the design of mutants of these proteins, the researchers have been able to observe the structure of one of the members of this protein family in atomic detail and decipher its function. (cnio.es)
  • Thanks to cryo-electron microscopy, a field in which Llorca is an international expert, visualisation of the molecular structure of proteins has taken a giant step towards what we now know as the golden age of 3D structures. (cnio.es)
  • The researchers combined x-ray crystallographic data, which gives 3-D atomic details of the constituent viral proteins, with cryo-electron microscopy images to determine how proteins in the T4 phage rearrange themselves during cell infection. (infectioncontroltoday.com)
  • NINDS Cryo-Electron Microscopy core surves many structural biology labs that use Cryo-EM single particle analysis to study the structures of proteins & macromolecules. (nih.gov)
  • High-resolution cryo-electron microscopy determines the atomic-scale structure of proteins and protein complexes to uncover the molecular mechanisms regulating cellular processes. (berstructuralbioportal.org)
Correlative4
  • The facility's expert team supports and collaborates on a large number of bio EM techniques ranging from standard SEM and TEM to immuno EM, correlative light and electron microscopy, cryo tomography and single particle analysis. (monash.edu)
  • Towards correlative super-resolution fluorescence and electron cryo-microscopy. (nih.gov)
  • Photonic-chip assisted correlative light and electron microscopy. (embl.org)
  • The CBS is offering a group leader position in cell structural biology for a specialist in cryo-electron microscopy, cryo-tomography and correlative a. (cnrs.fr)
Cryogenic3
  • Modified cryogenic electron microscopy can analyze viral structures better than ever before. (the-scientist.com)
  • In this project, we propose the use of state-of-art cryogenic electron microscopy techniques to track mineral formation in MVs and their interactions within the extracellular matrix. (fapesp.br)
  • Next, the team isolated chlorosomes from the mutant and the wild-type forms of the bacteria and used cryo-electron microscopy -- a type of electron microscopy that is performed at super-cold cryogenic temperatures -- to take pictures of the chlorosomes. (azooptics.com)
Macromolecular3
  • Cryo-electron microscopy (cryo-EM), combined with image processing, is an increasingly powerful tool for structure determination of macromolecular protein complexes and assemblies. (ox.ac.uk)
  • Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions. (imex-revista.com)
  • Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions. (gotonextstep.com)
Structures6
  • One method applied for that is cryo electron microscopy (cryo-EM), which can be used to make three-dimensional structures of biomolecules visible. (mpg.de)
  • Typically, it is believed that in order to solve structures of biological macromolecules at atomic resolution with cryo-electron microscopy (cryo-EM), a technique for which the 2017 Nobel Prize in chemistry was awarded, a high power microscope like the Krios must be employed. (yalescientific.org)
  • This year's prize went to the development of cryo-electron microscopy, a technique that allows the structures of biomolecules to be revealed where other techniques fail. (compoundchem.com)
  • Cryo-electron microscopy (Cryo-EM) analysis of the yeast 60S-RQC structures revealed that Rqc2 occupies a large proportion of the inter-subunit surface of 60S and prevents 40S-subunit reassociation after 80S splitting 10 , 12 , 29 . (nature.com)
  • used cryo-electron microscopy to look at structures of the M. tuberculosis RNA polymerase in different states, including when it was bound to fidaxomicin. (elifesciences.org)
  • Here, we report cryo-electron microscopy structures of Escherichia coli transcription-translation complexes (TTCs) containing different-length mRNA spacers between RNAP and the ribosome active-center P site. (cheric.org)
Facility9
  • The Monash Ramaciotti Centre is a leading facility for life sciences electron microscopy. (monash.edu)
  • In addition to the confocal microscopy, NMR spectroscopy and X-ray diffraction facilities at CCMB, the addition of cryo-electron microscopy makes it a remarkable facility for researchers to investigate details of living cells like never before. (pharmiweb.com)
  • Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. (ox.ac.uk)
  • LISCB hosts a Midlands Regional cryo-Electron Microscopy (cryo-EM) Facility. (le.ac.uk)
  • The facility provides advanced expertise in electron microscopy, from sample preparation to image analysis, for a wide variety of biological samples. (embl.org)
  • UT Southwestern's Cryo-Electron Microscopy Facility is supported by a grant from the Cancer Prevention & Research Institute of Texas. (utsouthwestern.edu)
  • The scientists' latest accomplishment hinged on the use of one of the highest-resolution transmission electron microscopes in the world, called TEAM I. It's located at the National Center for Electron Microscopy, which is a Molecular Foundry facility. (nextbigfuture.com)
  • Core Facility for Electron Microscopy, and all members of the resulting refined model and half map 2 (SSU-body focused) and EMD-11437-additional map 2. (thecoinradar.com)
  • The facility will house cryo-electron microscopy, powerful visualising equipment allowing researchers to examine diseases down to the finest detail, or the molecular and even atomic level. (edu.au)
Cryo-electron microsco1
  • Learning from terabytes of images, AI diagnostics can match clinicians in detecting pathologies in medical scan and X-ray data, while cryo-electron microscopy images can reveal 3D molecular structure within cells and viruses much faster than traditional X-ray diffraction techniques. (computerweekly.com)
Technique called cryo-electron m2
  • They used a technique called cryo-electron microscopy. (nih.gov)
  • To learn how these antibodies neutralize ebolaviruses, LJI Postdoctoral Fellows Xiaoying Yu, Ph.D., and Jake Milligan, Ph.D., spearheaded the use of an imaging technique called cryo-electron microscopy. (scitechdaily.com)
Light microscopy2
  • Because the wavelength of electrons [6] is so much shorter than light [7] , much finer detail can be obtained using electron microscopy than with light microscopy. (peterjamesthomas.com)
  • History of the electron microscope, spanning from the origins of light microscopy to modern techniques such as cryo-electron microscopy. (thermofisher.com)
Reconstructions2
  • The improved resolution allowed for model http://www.pafiry.net/cheap-nitrostat-canada/ building and refinement into electron cryo-microscopy reconstructions. (gotonextstep.com)
  • Although tropomyosin is well defined in electron microscopy helical reconstructions of thin filaments, troponin density is mostly lost. (infona.pl)
Protein8
  • We used the methods of cryo-electron microscopy with bioinformatics, protein design and expression, biochemistry and immunology for the development of a novel vaccine nanoplatform. (nih.gov)
  • But now, armed with a suite of new technology, cryo-electron microscopy has revealed the individual atoms in a protein - and how they really fit together. (freethink.com)
  • Both teams imaged apoferritin, a protein, Callaway reports, whose Steelix -like strength and stability makes it a popular cryo-electron microscopy subject. (freethink.com)
  • An image of the protein apoferritin created with cryo-electron microscopy. (freethink.com)
  • Cryo-electron microscopy (cryo-EM) data define the overall envelope of the assembly and rigid-body orientation of the subunits while solid-state nuclear magnetic resonance (ssNMR) chemical shifts and distance constraints define the local secondary structure, protein fold and inter-subunit interactions. (mpg.de)
  • Here, we introduce the nanoparticle-aided cryo-electron microscopy sampling (NACS) method to access the conformational distribution of a protein molecule. (ibs.re.kr)
  • By analyzing the chimpanzee adenovirus used by AstraZeneca with cryo-electron microscopy, which allows for molecular-level details to be seen, researchers found that the adenovirus attracts the blood protein known as "platelet factor 4" to it. (ctvnews.ca)
  • Structure of the LAT2/CD98hc protein, resolved by cryo-electron microscopy. (cnio.es)
Structural10
  • Congratulations to Jacques Dubochet, Joachim Frank, and Richard Henderson, who have received the 2017 Nobel Prize in Chemistry for developing cryo-electron microscopy, a technique that allows scientists to visualize the structural details of biological molecules and gain insights into how living systems function. (emdataresource.org)
  • Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. (ox.ac.uk)
  • Here, we propose a structural model of the yeast pontin/reptin complex based on a cryo-electron microscopy reconstruction at 13 A. Pontin/reptin hetero-dodecamers were purified from in vivo assembled complexes forming a double ring. (nih.gov)
  • Two nanogold particles are labeled at two target sites, and the interparticle distance is measured as a structural parameter via cryo-electron microscopy (cryo-EM). (ibs.re.kr)
  • The Rad51-DNA filaments are amenable to structural studies using cryo-electron microscopy (cryo-EM). (uthscsa.edu)
  • The SSCB utilizes advanced microscopy methods and cellular biology approaches to elucidate the molecular and structural basis of mechanisms underlying normal and disordered hearing and balance. (nih.gov)
  • Training in structural cell biology and advanced microscopy imaging. (nih.gov)
  • Structural analysis of the C. elegans dauer larval anterior sensilla by focused ion beam-scanning electron microscopy. (embl.org)
  • The combination of structural resolution by cryo-electron microscopy with molecular dynamics calculations and functional studies provides an experimental platform with a lot of potential that allows us to unravel the function of amino acid transporters. (cnio.es)
  • To identify the structural determinants of Fdx binding to RNAP, we determined the 3.4 Å cryo-electron microscopy structure of a complete M. tuberculosis RNAP holoenzyme in complex with Fdx. (elifesciences.org)
Biomolecules2
  • The Nobel Prize in Chemistry 2017 is awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for the development of cryo-electron microscopy, which both simplifies and improves the imaging of biomolecules. (peterjamesthomas.com)
  • Cryo-electron microscopy, which both simplifies and improves the imaging of biomolecules. (tutorialspoint.com)
Helical2
  • Structure of HIV-1 capsid assemblies by cryo-electron microscopy and iterative helical real-space reconstruction. (ox.ac.uk)
  • Here, we describe purification of full-length recombinant metastable helical nucleocapsid of Hantaan virus ( Hantaviridae family, Bunyavirales order) and determine its structure at 3.3 Å resolution by cryo-electron microscopy. (elifesciences.org)
Life sciences1
  • We've created the life sciences learning center to connect scientists around the world to the latest information on electron microscopy technology and to guide you to the best solution that will accelerate your next discovery or breakthrough. (thermofisher.com)
Atomic resolution1
  • Research teams at Germany's Max Planck Institute for Biophysical Chemistry and the Medical Research Council Laboratory of Molecular Biology in the U.K. have both recently pushed cryo-electron microscopy into atomic resolution. (freethink.com)
Computational1
  • Almost one-half of IRP labs use Biowulf to process and analyze their research data in areas such as genomics, computational chemistry, and cryo-electron microscopy imaging. (nih.gov)
Image3
  • We have determined the threedimensional structure of an amyloid fibril, formed by the SH3 domain of phosphatidylinositol-3′-kinase, using cryo-electron microscopy and image processing at 25 A resolution. (pasteur.fr)
  • The cryo-electron microscopy image of the chimpanzee adenovirus which delivers the Oxford-AstraZeneca vaccine. (ctvnews.ca)
  • EMAN2: an extensible image where can i buy cefzil processing suite for electron microscopy. (gisci.it)
High-resolution6
  • Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. (nih.gov)
  • Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology. (jic.ac.uk)
  • For more details, see the Request for Information (RFI): Screening for High Resolution Cryo-Electron Microscopy ( NOT-RM-21-012 ). (nih.gov)
  • High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy. (mpg.de)
  • These properties make the single and dual layer graphene films ideal for high resolution TEM imaging, nanoparticles imaging and cryo-electron microscopy. (ravescientific.com)
  • High-Resolution Cryoelectron Microscopy Structure of the Cyclic Nucleotide-Modulated Potassium Channel MloK1 in a Lipid Bilayer. (cornell.edu)
Receptor2
  • When trying their cryo-electron microscopy techniques on a simplified version of the GABAA receptor - target of anxiety meds and anesthetics, among other things - they saw details that had never been seen before, including water molecules at a site for histamine, a driving factor in allergic reactions. (freethink.com)
  • 카이스트(KAIST, 총장 이광형)는 생명과학과 송지준 교수 연구팀이 초저온 전자 현미경(cryo-Electron Microscopy)을 이용해 호르몬 조절 물질인 소마토스타틴(somatostatin)과 그 수용체인 소마토스타틴 리셉터 2(Somatostatin Receptor 2, SSTR2) 복합체의 3차원 원자 해상도 구조를 규명했다고 6일 발표했다. (kaist.ac.kr)
20171
  • Cryo-electron microscopy is a relatively new and revolutionary technology (according to Nature) that was awarded a Nobel Prize in 2017. (surf.nl)
Biochemistry1
  • The University of Wisconsin-Madison Department of Biochemistry will host the grand opening of its Cryo-Electron Microscopy Research Center (CEMRC) and Midwest Center for Cryo-Electron Tomography (MCCET) at 8:30 a.m. on June 7. (wisc.edu)
Electrons2
  • The "cryo" part of cryo-electron microscopy refers to the flash-freezing of the sample before you riddle it with electrons. (freethink.com)
  • Cryo-electron microscopy involves flash-freezing the study subject and then bombarding them with electrons to produce microscope images of individual molecules. (ctvnews.ca)
Data3
  • Singer is "being recognized for foundational contributions to mathematical data analysis and the mathematics of cryo-electron microscopy. (princeton.edu)
  • We were extremely fortunate to have extraordinarily talented scientists such as Petr Leiman and Victor Kostyuchenko as well as equally talented participation of Paul Chipman who did all the electron microscopy data collection, Rossmann added. (infectioncontroltoday.com)
  • Meet FAST-EM, a system that creates a fundamental shift in electron microscopy, where unprecedented large areas can be imaged at the nanoscale while freeing up time for operators to focus on data analysis and interpretation. (delmic.com)
Ribosome1
  • Cryo-electron microscopy analysis reveals that Hel2-bound ribosome are dominantly the rotated form with hybrid tRNAs. (nature.com)
Resolution4
  • Holger Stark from the Max Planck Institute for Biophysical Chemistry in Göttingen and his team have broken a crucial resolution barrier in cryo electron microscopy. (mpg.de)
  • The resolution revolution in cryo-electron microscopy]. (nih.gov)
  • At Leicester, we are developing cutting-edge super-resolution localisation microscopy. (le.ac.uk)
  • We have now determined the structure of these tubes at medium resolution (22 Å) by electron microscopy. (labex-gral.fr)
Molecules3
  • Using cryo-electron microscopy, the spatial structure and function of individual molecules can be studied very precisely. (computerweekly.com)
  • For more on cryo-EM see the blog post Cryo-Electron Microscopy Reveals Molecules in Ever Greater Detail . (nih.gov)
  • They used genetic techniques to create a mutant bacterium with a more regular internal structure, cryo-electron microscopy to identify the larger distance constraints for the chlorosome, solid-state nuclear magnetic resonance (NMR) spectroscopy to determine the structure of the chlorosome's component chlorophyll molecules, and modeling to bring together all of the pieces and create a final picture of the chlorosome. (azooptics.com)
Advances1
  • Recent advances in electron cryo microscopy have revolutionized how we address fundamental questions in cell and molecular biology. (snf.ch)
Negative stain1
  • We have studied the shape of myosin VI, the actin minus-end directed motor, by negative stain and metal shadow electron microscopy. (infona.pl)
Preparation2
  • Expertise in sample preparation for cryo-electron microscopy and cryo-electron tomography. (nih.gov)
  • The described methods are useful for sample preparation in crystallographic analysis and other structure determination techniques, such as cryo-electron microscopy. (portlandpress.com)
En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes | Nature Communications
En route to dynamic life processes by SNARE-mediated fusion of polymer and hybrid membranes | Nature Communications (nature.com)
Cooperative inhibition of SNARE-mediated vesicle fusion by α-synuclein monomers and oligomers | Scientific Reports
Cooperative inhibition of SNARE-mediated vesicle fusion by α-synuclein monomers and oligomers | Scientific Reports (nature.com)
Frontiers | Discovery of Exosomes From Tick Saliva and Salivary Glands Reveals Therapeutic Roles for CXCL12 and IL-8 in Wound...
Frontiers | Discovery of Exosomes From Tick Saliva and Salivary Glands Reveals Therapeutic Roles for CXCL12 and IL-8 in Wound... (frontiersin.org)
Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy | Nature
Backbone structure of the infectious ε15 virus capsid revealed by electron cryomicroscopy | Nature (nature.com)
Microscopic images and protein [IMAGE] | EurekAlert! Science News Releases
Microscopic images and protein [IMAGE] | EurekAlert! Science News Releases (eurekalert.org)
Muscle cells need calcium ions | Max-Planck-Gesellschaft
Muscle cells need calcium ions | Max-Planck-Gesellschaft (mpg.de)
PDF] Recent Progress of In Situ Transmission Electron Microscopy for Energy Materials | Semantic Scholar
PDF] Recent Progress of In Situ Transmission Electron Microscopy for Energy Materials | Semantic Scholar (semanticscholar.org)
Optimization of FIB-SEM Tomography and Reconstruction for Soft, Porous, and Poorly Conducting Materials | Microscopy and...
Optimization of FIB-SEM Tomography and Reconstruction for Soft, Porous, and Poorly Conducting Materials | Microscopy and... (cambridge.org)
A drug and ATP binding site in type 1 ryanodine receptor: Structure
A drug and ATP binding site in type 1 ryanodine receptor: Structure (cell.com)
Nano-capsule technology and Green Chemical Processes - Monash University
Nano-capsule technology and Green Chemical Processes - Monash University (research.monash.edu)
1gr5.1 | SWISS-MODEL Template Library
1gr5.1 | SWISS-MODEL Template Library (swissmodel.expasy.org)
Steven J Ludtke, Ph.D. | BCM
Steven J Ludtke, Ph.D. | BCM (bcm.edu)
Structure and gating mechanism of the transient receptor potential channel TRPV3 | Nature Structural & Molecular Biology
Structure and gating mechanism of the transient receptor potential channel TRPV3 | Nature Structural & Molecular Biology (nature.com)
Cryo-electron Microscopy Offers Atomic-Level Images of How Drug Binds to Cancer Protein
Cryo-electron Microscopy Offers Atomic-Level Images of How Drug Binds to Cancer Protein (cancernetwork.com)
Technology Hotels Archive - Page 10 of 16 - Dutch Techcentre for Life Sciences
Technology Hotels Archive - Page 10 of 16 - Dutch Techcentre for Life Sciences (dtls.nl)
All CryoEM articles | Chemistry World
All CryoEM articles | Chemistry World (chemistryworld.com)
SBWire - The Small Business Newswire | Press Releases
SBWire - The Small Business Newswire | Press Releases (sbwire.com)
Structures and pH-sensing mechanism of the proton-activated chloride channel | Nature
Structures and pH-sensing mechanism of the proton-activated chloride channel | Nature (nature.com)
Structural basis for electron transport mechanism of complex I-like photosynthetic NAD(P)H dehydrogenase | Nature Communications
Structural basis for electron transport mechanism of complex I-like photosynthetic NAD(P)H dehydrogenase | Nature Communications (nature.com)
Structure and dynamics of a mycobacterial type VII secretion system | Nature
Structure and dynamics of a mycobacterial type VII secretion system | Nature (nature.com)
Frontiers | Recent Developments in Data-Assisted Modeling of Flexible Proteins
Frontiers | Recent Developments in Data-Assisted Modeling of Flexible Proteins (frontiersin.org)
Matthew Libera | Stevens Institute of Technology
Matthew Libera | Stevens Institute of Technology (stevens.edu)
SMART: ZnF_C2H2 domain annotation
SMART: ZnF_C2H2 domain annotation (smart.embl.de)
Wissenschaftliche Publikationen | Max-Planck-Gesellschaft
Wissenschaftliche Publikationen | Max-Planck-Gesellschaft (mpg.de)
Frontiers | Teneurin Structures Are Composed of Ancient Bacterial Protein Domains
Frontiers | Teneurin Structures Are Composed of Ancient Bacterial Protein Domains (frontiersin.org)
Jason Van Rooyen - - Diamond Light Source
Jason Van Rooyen - - Diamond Light Source (diamond.ac.uk)