Cryoelectron Microscopy
Cryoultramicrotomy
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Models, Molecular
Atadenovirus
Negative Staining
The technique of washing tissue specimens with a concentrated solution of a heavy metal salt and letting it dry. The specimen will be covered with a very thin layer of the metal salt, being excluded in areas where an adsorbed macromolecule is present. The macromolecules allow electrons from the beam of an electron microscope to pass much more readily than the heavy metal; thus, a reversed or negative image of the molecule is created.
Image Processing, Computer-Assisted
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Models, Structural
Electron Microscope Tomography
Encephalomyelitis, Western Equine
A form of arboviral encephalitis (which primarily affects horses) endemic to western and central regions of NORTH AMERICA. The causative organism (ENCEPHALOMYELITIS VIRUS, WESTERN EQUINE) may be transferred to humans via the bite of mosquitoes (CULEX tarsalis and others). Clinical manifestations include headache and influenza-like symptoms followed by alterations in mentation, SEIZURES, and COMA. DEATH occurs in a minority of cases. Survivors may recover fully or be left with residual neurologic dysfunction, including PARKINSONISM, POSTENCEPHALITIC. (From Joynt, Clinical Neurology, 1996, Ch26, pp8-9)
Virion
Protein Structure, Quaternary
Crystallography, X-Ray
Tomography
Virus Assembly
Microscopy
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
Rhinovirus
Crystallization
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Imaging, Three-Dimensional
The process of generating three-dimensional images by electronic, photographic, or other methods. For example, three-dimensional images can be generated by assembling multiple tomographic images with the aid of a computer, while photographic 3-D images (HOLOGRAPHY) can be made by exposing film to the interference pattern created when two laser light sources shine on an object.
X-Ray Diffraction
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Cryopreservation
Immunoglobulin Fab Fragments
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
Binding Sites
Microscopy, Electron, Scanning
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Protein Structure, Secondary
Amino Acid Sequence
Macromolecular Substances
Microscopy, Confocal
Receptors, Virus
Protein Subunits
Dimerization
Protein Binding
Microscopy, Fluorescence
Endoplasmic Reticulum, Rough
Sequence Homology, Amino Acid
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Microscopy, Atomic Force
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
Microscopy, Electron, Transmission
Microscopy, Immunoelectron
Sindbis Virus
The type species of ALPHAVIRUS normally transmitted to birds by CULEX mosquitoes in Egypt, South Africa, India, Malaya, the Philippines, and Australia. It may be associated with fever in humans. Serotypes (differing by less than 17% in nucleotide sequence) include Babanki, Kyzylagach, and Ockelbo viruses.
Cryo-electron microscopy structure of an SH3 amyloid fibril and model of the molecular packing. (1/1790)
Amyloid fibrils are assemblies of misfolded proteins and are associated with pathological conditions such as Alzheimer's disease and the spongiform encephalopathies. In the amyloid diseases, a diverse group of normally soluble proteins self-assemble to form insoluble fibrils. X-ray fibre diffraction studies have shown that the protofilament cores of fibrils formed from the various proteins all contain a cross-beta-scaffold, with beta-strands perpendicular and beta-sheets parallel to the fibre axis. We have determined the threedimensional structure of an amyloid fibril, formed by the SH3 domain of phosphatidylinositol-3'-kinase, using cryo-electron microscopy and image processing at 25 A resolution. The structure is a double helix of two protofilament pairs wound around a hollow core, with a helical crossover repeat of approximately 600 A and an axial subunit repeat of approximately 27 A. The native SH3 domain is too compact to fit into the fibril density, and must unfold to adopt a longer, thinner shape in the amyloid form. The 20x40-A protofilaments can only accommodate one pair of flat beta-sheets stacked against each other, with very little inter-strand twist. We propose a model for the polypeptide packing as a basis for understanding the structure of amyloid fibrils in general. (+info)MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member. (2/1790)
Terminal cell differentiation is correlated with the extensive sequestering of previously active genes into compact transcriptionally inert heterochromatin. In vertebrate blood cells, these changes can be traced to the accumulation of a developmentally regulated heterochromatin protein, MENT. Cryoelectron microscopy of chicken granulocyte chromatin, which is highly enriched with MENT, reveals exceptionally compact polynucleosomes, which maintain a level of higher order folding above that imposed by linker histones. The amino acid sequence of MENT reveals a close structural relationship with serpins, a large family of proteins known for their ability to undergo dramatic conformational transitions. Conservation of the "hinge region" consensus in MENT indicates that this ability is retained by the protein. MENT is distinguished from the other serpins by being a basic protein, containing several positively charged surface clusters, which are likely to be involved in ionic interactions with DNA. One of the positively charged domains bears a significant similarity to the chromatin binding region of nuclear lamina proteins and with the A.T-rich DNA-binding motif, which may account for the targeting of MENT to peripheral heterochromatin. MENT ectopically expressed in a mammalian cell line is transported into nuclei and is associated with intranuclear foci of condensed chromatin. (+info)Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules. (3/1790)
gamma-tubulin exists in two related complexes in Drosophila embryo extracts (Moritz, M., Y. Zheng, B.M. Alberts, and K. Oegema. 1998. J. Cell Biol. 142:1- 12). Here, we report the purification and characterization of both complexes that we name gamma-tubulin small complex (gammaTuSC; approximately 280,000 D) and Drosophila gammaTuRC ( approximately 2,200,000 D). In addition to gamma-tubulin, the gammaTuSC contains Dgrip84 and Dgrip91, two proteins homologous to the Spc97/98p protein family. The gammaTuSC is a structural subunit of the gammaTuRC, a larger complex containing about six additional polypeptides. Like the gammaTuRC isolated from Xenopus egg extracts (Zheng, Y., M.L. Wong, B. Alberts, and T. Mitchison. 1995. Nature. 378:578-583), the Drosophila gammaTuRC can nucleate microtubules in vitro and has an open ring structure with a diameter of 25 nm. Cryo-electron microscopy reveals a modular structure with approximately 13 radially arranged structural repeats. The gammaTuSC also nucleates microtubules, but much less efficiently than the gammaTuRC, suggesting that assembly into a larger complex enhances nucleating activity. Analysis of the nucleotide content of the gammaTuSC reveals that gamma-tubulin binds preferentially to GDP over GTP, rendering gamma-tubulin an unusual member of the tubulin superfamily. (+info)Cryoelectron microscopy of a nucleating model bile in vitreous ice: formation of primordial vesicles. (4/1790)
Because gallstones form so frequently in human bile, pathophysiologically relevant supersaturated model biles are commonly employed to study cholesterol crystal formation. We used cryo-transmission electron microscopy, complemented by polarizing light microscopy, to investigate early stages of cholesterol nucleation in model bile. In the system studied, the proposed microscopic sequence involves the evolution of small unilamellar to multilamellar vesicles to lamellar liquid crystals and finally to cholesterol crystals. Small aliquots of a concentrated (total lipid concentration = 29.2 g/dl) model bile containing 8.5% cholesterol, 22.9% egg yolk lecithin, and 68.6% taurocholate (all mole %) were vitrified at 2 min to 20 days after fourfold dilution to induce supersaturation. Mixed micelles together with a category of vesicles denoted primordial, small unilamellar vesicles of two distinct morphologies (sphere/ellipsoid and cylinder/arachoid), large unilamellar vesicles, multilamellar vesicles, and cholesterol monohydrate crystals were imaged. No evidence of aggregation/fusion of small unilamellar vesicles to form multilamellar vesicles was detected. Low numbers of multilamellar vesicles were present, some of which were sufficiently large to be identified as liquid crystals by polarizing light microscopy. Dimensions, surface areas, and volumes of spherical/ellipsoidal and cylindrical/arachoidal vesicles were quantified. Early stages in the separation of vesicles from micelles, referred to as primordial vesicles, were imaged 23-31 min after dilution. Observed structures such as enlarged micelles in primordial vesicle interiors, segments of bilayer, and faceted edges at primordial vesicle peripheries are probably early stages of small unilamellar vesicle assembly. A decrease in the mean surface area of spherical/ellipsoidal vesicles was correlated with the increased production of cholesterol crystals at 10-20 days after supersaturation by dilution, supporting the role of small unilamellar vesicles as key players in cholesterol nucleation and as cholesterol donors to crystals. This is the first visualization of an intermediate structure that has been temporally linked to the development of small unilamellar vesicles in the separation of vesicles from micelles in a model bile and suggests a time-resolved system for further investigation. (+info)Native display of complete foreign protein domains on the surface of hepatitis B virus capsids. (5/1790)
The nucleocapsid of hepatitis B virus (HBV), or HBcAg, is a highly symmetric structure formed by multiple dimers of a single core protein that contains potent T helper epitopes in its 183-aa sequence. Both factors make HBcAg an unusually strong immunogen and an attractive candidate as a carrier for foreign epitopes. The immunodominant c/e1 epitope on the capsid has been suggested as a superior location to convey high immunogenicity to a heterologous sequence. Because of its central position, however, any c/e1 insert disrupts the core protein's primary sequence; hence, only peptides, or rather small protein fragments seemed to be compatible with particle formation. According to recent structural data, the epitope is located at the tips of prominent surface spikes formed by the very stable dimer interfaces. We therefore reasoned that much larger inserts might be tolerated, provided the individual parts of a corresponding fusion protein could fold independently. Using the green fluorescent protein (GFP) as a model insert, we show that the chimeric protein efficiently forms fluorescent particles; hence, all of its structurally important parts must be properly folded. We also demonstrate that the GFP domains are surface-exposed and that the chimeric particles elicit a potent humoral response against native GFP. Hence, proteins of at least up to 238 aa can be natively displayed on the surface of HBV core particles. Such chimeras may not only be useful as vaccines but may also open the way for high resolution structural analyses of nonassembling proteins by electron microscopy. (+info)Flexibility of the major antigenic loop of foot-and-mouth disease virus bound to a Fab fragment of a neutralising antibody: structure and neutralisation. (6/1790)
The interaction of foot-and-mouth disease virus (FMDV) serotype C (clone C-S8c1) with a strongly neutralising monoclonal antibody (MAb) 4C4 has been studied by combining data from cryoelectron microscopy and x-ray crystallography. The MAb 4C4 binds to the exposed flexible GH-loop of viral protein 1 (VP1), which appears to retain its flexibility, allowing movement of the bound Fab. This is in striking contrast to MAb SD6, which binds to the same GH-loop of VP1 but exhibits no movement of the bound Fab when observed under identical conditions. However, MAbs 4C4 and SD6 have very similar neutralisation characteristics. The known atomic structure of FMDV C-S8c1 and that of the 4C4 Fab cocrystallised with a synthetic peptide corresponding to the GH-loop of VP1 were fitted to the cryoelectron microscope density map. The best fit of the 4C4 Fab is compatible only with monovalent binding of the MAb in agreement with the neutralisation data on 4C4 MAbs, Fab2s, and Fabs. The position of the bound GH-loop is related to other known positions of this loop by a hinge rotation about the base of the loop. The 4C4 Fab appears to interact almost exclusively with the G-H loop of VP1, making no other contacts with the viral capsid. (+info)Visualization of tegument-capsid interactions and DNA in intact herpes simplex virus type 1 virions. (7/1790)
Herpes simplex virus type 1 virions were examined by electron cryomicroscopy, allowing the three-dimensional structure of the infectious particle to be visualized for the first time. The capsid shell is identical to that of B-capsids purified from the host cell nucleus, with the exception of the penton channel, which is closed. The double-stranded DNA genome is organized as regularly spaced ( approximately 26 A) concentric layers inside the capsid. This pattern suggests a spool model for DNA packaging, similar to that for some bacteriophages. The bulk of the tegument is not icosahedrally ordered. However, a small portion appears as filamentous structures around the pentons, interacting extensively with the capsid. Their locations and interactions suggest possible roles for the tegument proteins in regulating DNA transport through the penton channel and binding to cellular transport proteins during viral infection. (+info)Effect of buffer conditions on the position of tRNA on the 70 S ribosome as visualized by cryoelectron microscopy. (8/1790)
The effect of buffer conditions on the binding position of tRNA on the Escherichia coli 70 S ribosome have been studied by means of three-dimensional (3D) cryoelectron microscopy. Either deacylated tRNAfMet or fMet-tRNAfMet were bound to the 70 S ribosomes, which were programmed with a 46-nucleotide mRNA having AUG codon in the middle, under two different buffer conditions (conventional buffer: containing Tris and higher Mg2+ concentration [10-15 mM]; and polyamine buffer: containing Hepes, lower Mg2+ concentration [6 mM], and polyamines). Difference maps, obtained by subtracting 3D maps of naked control ribosome in the corresponding buffer from the 3D maps of tRNA.ribosome complexes, reveal the distinct locations of tRNA on the ribosome. The position of deacylated tRNAfMet depends on the buffer condition used, whereas that of fMet-tRNAfMet remains the same in both buffer conditions. The acylated tRNA binds in the classical P site, whereas deacylated tRNA binds mostly in an intermediate P/E position under the conventional buffer condition and mostly in the position corresponding to the classical P site, i. e. in the P/P state, under the polyamine buffer conditions. (+info)
ASTM E3143 - 18b Standard Practice for Performing Cryo-Transmission Electron Microscopy of Liposomes
Single-step antibody-based affinity cryo-electron microscopy for imaging and structural analysis of macromolecular assemblies<...
ASSISTANT PROFESSOR IN CRYO ELECTRON MICROSCOPY job with University of California, Irvine | 417876
Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs | SpringerLink
Improved methods for single-particle cryogenic electron microscopy
Crucial steps in the structure determination of a coronavirus spike glycoprotein using cryo-electron microscopy | Research -...
Structural markers in prostate cancer serum imaged ex vivo using cryogenic transmission electron microscopy
Resolving the Morphology of Peptoid Vesicles at the 1 nm Length Scale Using Cryogenic Electron Microscopy.
Structural studies of virus-antibody complexes by electron cryomicroscopy and X-ray crystallography<...
RCSB PDB - 3JBP: Cryo-electron microscopy reconstruction of the Plasmodium falciparum 80S ribosome bound to E-tRNA
Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics. - Nuffield Department of Medicine
The resolution revolution continues: GIF Quantum LS imaging filter with K2 Summit camera enables scientists to reach 2.2 Å...
Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome.
Using molecular approaches to understand Complex I deficiency in mouse models
High resolution single-particle cryo-EM of a 200 kDa membrane ion channel | NIH Research Festival
Cryo Electron Microscopy Short Course 2010 | IPRIME
Solubilization of nonionic monoalkyl amphiphile-cholesterol vesicles by octyl glucoside: Cryo-transmission electron microscopy...
High resolution cryo-electron microscopy of clathrin cage networks at University of Warwick on FindAPhD.com
Cryo-EM structure of oxysterol-bound human Smoothened coupled to a heterotrimeric G<sub>i</sub> ...
Localization of eukaryote-specific ribosomal proteins in a 5.5-Å cryo-EM map of the 80S eukaryotic ribosome<...
ccp4bb] EMBO practical course on image processing for cryo-electron microscopy
single-particle electron cryomicroscopy Archives | The Emory Wheel
Cryo electron microscopy of membrane proteins and biomolecular assemblies - Hans Hebert | Department of Biosciences and...
CryoEM Structure Refinement by Integrating NMR Chemical Shifts with Molecular Dynamics Simulations. - Nuffield Department of...
Single particle analysis - Wikipedia
Single-particle cryo-EM of the ryanodine receptor from skeletal muscle by Alex H. Will
A Bayesian adaptive basis algorithm for single particle reconstruction. - PubMed - NCBI
Events - Instruct-ERIC
Stringent Response: Tet(O) cryoEM structure is out!
PDB-6id0: Cryo-EM structure of a human intron lariat spliceosome prior to P... - Yorodumi
PDB-5n5n: Cryo-EM structure of tsA201 cell alpha1B and betaI and betaIVb mi... - Yorodumi
News : EMDataResource
How smart, ultrathin nanosheets go fishing for proteins | Max Planck Institute of Biophysics
Spectroscopy Society of Pittsburgh » February Monthly Meeting
Forschungszentrum Jülich - Ansprechpartner - Prof. Dr. Carsten Sachse
Electron cryomicroscopy shows cardiac thin filament structure -- MEDICA - World Forum for Medicine
APPROACHING VIRUS LIFE CYCLE WITH ELECTRON MICROSCOPY | IMRI
Cryoelectron Microscopy of Icosahedral Virus Particles | SpringerLink
Zinc in the structure of Drosophila Nod (3DC4) and Bovine Tubulin (1JFF) Docked Into The 11-Angstrom Cryo-Em Map of Nucleotide...
Publications
New indication of a link between Alzheimers and diabetes | EurekAlert! Science News
Scientists take cool look at dendrite growth - Materials Today
Mapping the Structure and Function of the E1 and E2 Glycoproteins in Alphaviruses - CaltechAUTHORS
Cryo-electron microscopy of viruses | Gelbart Lab
First appointment in new Salk Fellows Program - Salk Institute for Biological Studies
Cryo-EM structure of a licensed DNA replication origin | Crick
Worlds leading supplier of electron microscopy solutions partners with Imaging CoE for R&D - Imaging CoE
Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics - Fingerprint
- University of...
Phasing of the Triatoma virus diffraction data using a cryo-electron microscopy reconstruction. - Institut Pasteur
Cryo Electron Microscopy and Electron Tomography - Drug Discovery World (DDW)
Cryo-electron tomography of cells: connecting structure and function :: MPG.PuRe
Combined Cryogenic Transmission Electron Microscopy and Electron Spin Resonance Studies of Egg Phosphatidylchloline Liposomes...
Cryo-electron Microscopy Structure of the Native Prototype Foamy Virus Glycoprotein and Virus Architecture - Université...
Cryo Electron Microscopy scoops Nobel Prize in Chemistry. | Department of Political Studies
Insights into bunyavirus architecture from electron cryotomography of Uukuniemi virus
Delmic Microscopy Blog | cryo-electron tomography
Rapid tilt-series method for cryo-electron tomography: Characterizing stage behavior during FISE acquisition - CaltechAUTHORS
Simulating cryo electron tomograms of crowded cell cytoplasm for assessment of automated particle picking | BMC Bioinformatics ...
Joachim Frank Wins Nobel Prize in Chemistry
Structural studies of human immunodeficiency virus type 1 by cryo-electron tomography - CaltechTHESIS
Bsoft: References
Electron Crystallography of Soluble and Membrane Proteins - beck-shop.de
Cryoelectron tomography - Latest research and news | Nature
Structure of the full-length HCV IRES in solution
Structure and conformational states of the bovine mitochondrial ATP synthase by cryo-EM. | MRC Mitochondrial Biology Unit
Protein structure could unlock new treatments for cystic fibrosis - Scienmag: Latest Science and Health News
Capsids of tricorn protease studied by electron cryomicroscopy :: MPG.PuRe
Coacervation of Elastin-Like Recombinamer Microgels
Cryo-EM structure of the small subunit of the mammalian mitochondrial ribosome. « RNomics
Soft cellulose II nanospheres: sol gel behaviour, swelling and material synthesisBeaumont, Marco; Rosenfeldt, Sabine; Tardy,...
Project abstract for group mccormic | Minnesota Supercomputing Institute
Averaging of Viral Envelope Glycoprotein Spikes from Electron Cryotomography Reconstructions using Jsubtomo - Oxford Particle...
Ariane Briegel - Leiden University
Cryo-EM reconstruction of the giant Mimivirus using C5 symmetry | NIH 3D Print Exchange
Viruses | Free Full-Text | Fluorescent Protein-Tagged Sindbis Virus E2 Glycoprotein Allows Single Particle Analysis of Virus...
Horizontal membrane-intrinsic α-helices in the stator a-subunit of an F-type ATP synthase. | MRC Mitochondrial Biology Unit
First atomic model of human telomerase constructed by electron cryo-microscopy
Dirksen E. Bussiere | ACS Division of Biological Chemistry Website
The Stephen Long Lab: Publications | Sloan Kettering Institute
Freezing immunoglobulins to see them move
Virus caught in the act of infecting a cell
Spatial Localization of the Ebola Virus Glycoprotein Mucin-Like Domain Determined by Cryo-Electron Tomography
HIV-1 Biogenesis Studied by Cellular Cryo-Electron Tomography and Biochemical in vitro Reconstitution - ORA - Oxford University...
Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations -...
Glycoprotein Ib clustering in platelets can be inhibited by α-linolenic acid as revealed by cryo-electron tomography |...
Conveyor technology: Non-Contact Optical Vibration Measurement with Subnanometer Resolution
Multivariate Statistical Analysis of Large Datasets: Single Particle Electron Microscopy
Structural properties of AMP-activated protein kinase: dimerization, molecular shape, and changes upon ligand binding.
Magnetic imaging with a Zernike-type phase plate in a transmission electron microscope
Undergraduate | Stanford EE
Ribosome and ribosomal RNA Structure : An experimental and computational analysis of expansion segments in eukaryotic ribosomal...
Colloquia - Math
Colloquia - Math
Transmission electron cryomicroscopy
Presentation on Cryoelectron Microscopy , PharmaXChange.info Shi D, Nannenga BL, Iadanza MG, Gonen T (November 2013). "Three- ... Adrian M, Dubochet J, Lepault J, McDowall AW (1984). "Cryo-electron microscopy of viruses". Nature. 308 (5954): 32-6. Bibcode: ... Murata K, Wolf M (Feb 2018). "Cryo-electron microscopy for structural analysis of dynamic biological macromolecules". ... "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution". The ...
SnRNP
Several human and yeast snRNP structures were determined by the cryo-electron microscopy and successive single particle ... Stark, Holger; Reinhard Lührmann (2006). "Cryo-Electron Microscopy of Spliceosomal Components". Annual Review of Biophysics and ... The structures determined by single particle electron microscopy analysis are: human U1 snRNP, human U11/U12 di-snRNP, human U5 ...
Deborah F. Kelly
"Cryo-Electron Microscopy in Cancer Research". Accelerating Microscopy. 2021-09-16. Retrieved 2022-04-08. "Celebrating Women in ... "Microchip-Based Toolkit to Complement Protein Analysis Using Cryo-Electron Microscopy - Nanoscale & Nanoscale Advances Blog". ... "About MSA - Council". Microscopy Society of America. Retrieved 2022-04-08. (Articles with short description, Short description ... Kelly was elected President of the Microscopy Society of America in 2022. Merryn Voysey; Sue Ann Costa Clemens; Shabir A Madhi ...
Lorena S. Beese
X-ray crystallography and cryo-electron microscopy; structure-based drug design; protein-protein and protein-nucleic acid ...
Cryofixation
Dubochet J, Adrian M, Chang JJ, Homo JC, Lepault J, McDowall AW, Schulz P (1988). "Cryo-electron microscopy of vitrified ... of biological materials as the first step in specimen preparation for electron microscopy and cryo-electron microscopy. Typical ... "The construction and operation of a simple and inexpensive slam freezing device for electron microscopy". Journal of Microscopy ... Ryan, Keith P. (1992). "Cryofixation of tissues for electron microscopy: a review of plunge cooling methods" (PDF). Scan. ...
Amorphous ice
... is used in some scientific experiments, especially in cryo-electron microscopy of biomolecules. The individual ... "Cryo-electron microscopy of vitrified specimens" (PDF). Quarterly Reviews of Biophysics. 21 (2): 129-228. doi:10.1017/ ...
4D scanning transmission electron microscopy
"A Primer to Single-Particle Cryo-Electron Microscopy". Cell. 161 (3): 438-449. doi:10.1016/j.cell.2015.03.050. PMC 4409659. ... 4D scanning transmission electron microscopy (4D STEM) is a subset of scanning transmission electron microscopy (STEM) which ... Electron beam induced deposition Transmission electron microscopy (TEM) Scanning Transmission Electron Microscopy (STEM) Energy ... Scanning confocal electron microscopy (SCEM) Scanning electron microscope (SEM) Detectors for transmission electron microscopy ...
Detectors for transmission electron microscopy
"A Primer to Single-Particle Cryo-Electron Microscopy". Cell. 161 (3): 438-449. doi:10.1016/j.cell.2015.03.050. ISSN 0092-8674. ... "Design and Characterization of 64 MegaPixel Fiber Optic Coupled CMOS Detector for Transmission Electron Microscopy". Microscopy ... "High Dynamic Range Pixel Array Detector for Scanning Transmission Electron Microscopy". Microscopy and Microanalysis. 22 (1): ... There are two main types of direct electron detector, both of which were first introduced to electron microscopy in the 2000s. ...
Joachim Frank
He is regarded as the founder of single-particle cryo-electron microscopy (cryo-EM), for which he shared the Nobel Prize in ... "Nobel Prize in Chemistry Awarded for Cryo-Electron Microscopy". The New York Times. October 4, 2017. Retrieved October 4, 2017 ... "The 16th Annual Wiley Prize in Biomedical Sciences Awarded for Pioneering Developments in Electron Microscopy". newsroom.wiley. ... Frank, Joachim (2009). "Single-particle reconstruction of biological macromolecules in electron microscopy -- 30 years". Q. Rev ...
Electron cryotomography
Zhang, Peijun (2013-10-01). "Correlative cryo-electron tomography and optical microscopy of cells". Current Opinion in ... One solution is to apply correlated cryo-fluorescence light microscopy, and even super-resolution light microscopy (e.g. cryo- ... Electron microscopy Electron tomography Transmission electron cryomicroscopy Transmission electron microscopy Gan, Lu; Jensen, ... "Cryo-electron microscopy of vitreous sections". The EMBO Journal. 23 (18): 3583-3588. doi:10.1038/sj.emboj.7600366. ISSN 0261- ...
EM Data Bank
Cryo-Electron Microscopy Colocalization Benchmark Source Lawson, Catherine L; Baker, Matthew L; Best, Christoph; et al. ( ... "Electron Microscopy Data Bank". Electron Microscopy Data Bank. Retrieved 2021-08-05. Esquivel-Rodríguez, J; Xiong, Y; Han, X; ... Software tools for molecular microscopy EM Data Bank US and UK Search pages Japan Search page (EM Navigator in PDBj) (Articles ... The EM Data Bank or Electron Microscopy Data Bank (EMDB) collects 3D EM maps and associated experimental data determined using ...
Jacques Dubochet
Dubochet developed technologies in cryo-electron microscopy, cryo-electron tomography and cryo-electron microscopy of vitreous ... "Nobel Prize in Chemistry Awarded for Cryo-Electron Microscopy". The New York Times. 4 October 2017. Retrieved 4 October 2017. ... Dubochet, J.; McDowall, A. W. (December 1981). "Vitrification of pure water for electron microscopy". Journal of Microscopy. ... Blog Jacques Dubochet on Nobelprize.org including the Nobel Lecture on 8 December 2017 Early cryo-electron microscopy Portals: ...
Smallpox
"Structure of intracellular mature vaccinia virus observed by cryoelectron microscopy". Journal of Virology. 68 (3): 1935-41. ... All orthopoxviruses exhibit identical brick-shaped virions by electron microscopy. If particles with the characteristic ...
Fusion mechanism
"Cryo-electron microscopy structure of a coronavirus spike glycoprotein trimer". Nature. 531 (7592): 114-117. Bibcode:2016Natur. ...
Ronny Hadani
Viewing angle classification of cryo-electron microscopy images using eigenvectors, A Singer, Z Zhao, Y Shkolnisky, R Hadani, ... Singer, A.; Zhao, Z.; Shkolnisky, Y.; Hadani, R. (2011-01-01). "Viewing Angle Classification of Cryo-Electron Microscopy Images ... Ronny Hadani Representation Theoretic Patterns in 3D Cryo-Electron Microscopy - Prof. Ronny Hadani (CS1 maint: url-status, ... "Representation theoretic patterns in three dimensional Cryo-Electron Microscopy I: The intrinsic reconstitution algorithm". ...
Single particle analysis
Using cryo-electron microscopy it has become possible to generate reconstructions with sub-nanometer resolution and near-atomic ... "Towards atomic resolution structural determination by single-particle cryo-electron microscopy". Current Opinion in Structural ... Transmission electron microscopy images are projections of the object showing the distribution of density through the object, ... EM Data Bank (EM Data Bank) Frank, Joachim (2006). Three-dimensional electron microscopy of macromolecular assemblies: ...
Epithelial sodium channel
"Structure of the human epithelial sodium channel by cryo-electron microscopy". eLife. 7: e39340. doi:10.7554/eLife.39340. PMC ... "Structure of the human epithelial sodium channel by cryo-electron microscopy". eLife. 7: e39340. doi:10.7554/eLife.39340. PMC ...
Chlorosome
Cryo-electron microscopy was used to take pictures of the chlorosomes. The images reveal that the chlorophyll molecules inside ... Genetic techniques to create a mutant bacterium with a more regular internal structure, cryo-electron microscopy to identify ... The combination of NMR, cryo-electron microscopy and modeling enabled the scientists to determine that the chlorophyll ... "Determination of the topography and biometry of chlorosomes by atomic force microscopy". Photosynthesis Research. 71 (1-2): 83- ...
Foldit
November 2019). "Building de novo cryo-electron microscopy structures collaboratively with citizen scientists". PLOS Biology. ...
Bacteriophage pRNA
2001). "Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi29". J. Struct. Biol. 135 (1): ...
Protein structure
For larger protein complexes, cryo-electron microscopy can determine protein structures. The resolution is typically lower than ... cryo electron microscopy (cryo-EM) and dual polarisation interferometry to determine the structure of proteins. Protein ...
Protein
Since then, cryo-electron microscopy (cryo-EM) of large macromolecular assemblies has been developed. Cryo-EM uses protein ... Cryoelectron microscopy is used to produce lower-resolution structural information about very large protein complexes, ... Zhou ZH (April 2008). "Towards atomic resolution structural determination by single-particle cryo-electron microscopy". Current ... This technique also uses an antibody to the protein of interest, along with classical electron microscopy techniques. The ...
Kiyoshi Nagai
From 2014, Nagai's group used cryo-electron microscopy to study the spliceosome. Structures of the U5.U4/U6 tri-snRNP gave the ... Fica, Sebastian M.; Nagai, Kiyoshi (2017-10-05). "Cryo-electron microscopy snapshots of the spliceosome: structural insights ... Insights from Cryo-Electron Microscopy Structures". Chemical Reviews. 118 (8): 4156-4176. doi:10.1021/acs.chemrev.7b00499. ISSN ...
Cation diffusion facilitator
2013) used cryoelectron microscopy to determine a 13 Å resolution structure of a YiiP homolog from Shewanella oneidensis within ... "Inward-facing conformation of the zinc transporter YiiP revealed by cryoelectron microscopy". Proceedings of the National ...
Electron microscope
An entire field called cryo-electron microscopy has branched from this technique. With the development of cryo-electron ... "Vitrification of cryoelectron microscopy specimens revealed by high-speed photographic imaging". Journal of Microscopy. 211 (1 ... Adrian, Marc; Dubochet, Jacques; Lepault, Jean; McDowall, Alasdair W. (1984). "Cryo-electron microscopy of viruses". Nature ( ... Danilatos, G.D. (1986). "Environmental scanning electron microscopy in colour". Journal of Microscopy. 142: 317-325. doi: ...
Michael Rossmann
Using cryo-electron microscopy, in 2016 his lab reported the first known structure of the Zika virus, responsible for a severe ... using a combination of cryo-electron microscopy and X-ray crystallography. Rossmann also had a long-term interest in ... "Visualization of membrane protein domains by cryo-electron microscopy of dengue virus". Nature Structural Biology. 10 (11): 907 ...
Sapovirus
"Antigenic and Cryo-Electron Microscopy Structure Analysis of a Chimeric Sapovirus Capsid". Journal of Virology. 90 (5): 2664- ... Assembly of new virus particles and release by cell lysis." Using electron microscopy, the sapovirus was first seen in ...
DNA nanotechnology
... transmission electron microscopy and cryo-electron microscopy are often used in this case. Extended three-dimensional lattices ... The proposed nanoparticle was characterized by atomic force microscopy (AFM), transmission electron microscopy (TEM) and ... Nucleic acid structures can be directly imaged by atomic force microscopy, which is well suited to extended two-dimensional ... "Designed two-dimensional DNA Holliday junction arrays visualized by atomic force microscopy". Journal of the American Chemical ...
Fourier shell correlation
In cryo-electron microscopy, the two volumes are the result of two three-dimensional reconstructions, each based on half of the ... The FSC originated in cryo-electron microscopy and gradually proliferated to other fields. To measure the FSC, two ... Frank, J. (2006). Three-Dimensional Electron Microscopy of Macromolecular Assemblies. New York: Oxford University Press. ISBN 0 ... "The Electron Microscopy Data Bank". www.ebi.ac.uk. Retrieved 2019-01-07. Harauz, G.; van Heel M. (1986). "Exact filters for ...
Densovirinae
... determined by cryo-electron microscopy". Journal of Molecular Biology. 347 (4): 791-801. doi:10.1016/j.jmb.2005.02.009. PMID ...
Bacillus virus phi29
employed cryo-electron microscopy to determine that, in vivo, only a pentamer or smaller polymer could spatially fit in the ... but these studies had a solely genetic basis rather than a microscopy based approach. In the year 2000, a study by Simpson et ...
Klaus Schulten
A practical guide to combine cryo-electron microscopy and X-ray crystallography". Methods. 49 (2): 174-180. doi:10.1016/j.ymeth ... using molecular dynamics in combination with cryo-electron microscopy and X-ray crystallography. to study the structures of ... "Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics". Nature. 497 (7451): 643-6. Bibcode: ...
Telomerase
Two years later, the first cryo-electron microscopy (cryo-EM) structure of telomerase holoenzyme (Tetrahymena) was determined. ... The negative stain electron microscopy (EM) structures of human and Tetrahymena telomerases were characterized in 2013. ...
Picornavirus
... using X-ray crystallography and cryoelectron microscopy. Depending on the type and degree of dehydration, the viral particle is ...
Spectral signal-to-noise ratio
The SSNR is a popular method for finding the resolution of a class average in cryo-electron microscopy. S S N R ( r ) = ∑ r i ... Frank, Joachim (2006). Three-Dimnsional Electron Microscopy of Macromolecular Assemblies. New York: Oxford University Press. ... Journal of Microscopy. 127 (2): 127-138. doi:10.1111/j.1365-2818.1982.tb00405.x. PMID 7120365. S2CID 27206060. Böttcher, B.; ... "Determination of the fold of the core protein of hepatitis B virus by electron microscopy". Nature. 386 (6620): 88-91. doi: ...
RasMol
... or cryo-electron microscopy. Rasmol can communicate with other programs via Tcl/Tk on Unix platforms, and via Dynamic Data ...
Electron tomography
In the field of biology, bright-field transmission electron microscopy (BF-TEM) and high-resolution TEM (HRTEM) are the primary ... Tomography Tomographic reconstruction Cryo-electron tomography Positron emission tomography Crowther criterion X-ray computed ... Midgley, P. A.; Weyland, M. (2003). "3D electron microscopy in the physical sciences: The development of Z-contrast and EFTEM ... Bals, S.; Kisielowski, C. F.; Croitoru, M.; Tendeloo, G. V. (2005). "Annular Dark Field Tomography in TEM". Microscopy and ...
Kinesin-like protein KIF11
X-ray crystallography, cryo-electron microscopy, and real-time infrared spectroscopy have been used to measure the structure of ...
List of German inventors and discoverers
Shared the Nobel Prize for Medicine 1956 Joachim Frank: co-invented cryo-electron microscopy. Shared the Nobel Prize for ... 600 patents in fields including television and radio, electron microscopy, medical technology, nuclear technology, and plasma ...
Mark Schlissel
... cryo-electron microscopy and RNA-based therapeutics, in addition to several exploratory grants for earlier-phase research. As ...
Vault (organelle)
They measure 34 nm by 60 nm from a negative stain, 26 nm by 49 nm from cryo-electron microscopy, and 35 nm by 59 nm from STEM. ...
Nucleoprotein
... nuclear magnetic resonance and cryo-electron microscopy. Virus genomes (either DNA or RNA) are extremely tightly packed into ...
Bsoft
Heymann JB, Cardone G, Winkler DC and Steven AC (2008) Computational resources for cryo-electron tomography in Bsoft. Journal ... EM Data Bank Bsoft home page Software Tools For Molecular Microscopy (Articles with short description, Short description is ... Heymann JB and Belnap DM (2007) Bsoft: Image processing and molecular modeling for electron microscopy. Journal of Structural ... Image and molecular processing in electron microscopy. Journal of Structural Biology 133(2/3), 156 - 169. ...
Valosin-containing protein
"Motions and negative cooperativity between p97 domains revealed by cryo-electron microscopy and quantised elastic deformational ...
Resolution (electron density)
In cryo-electron microscopy, resolution is typically measured by the Fourier shell correlation (FSC), a three-dimensional ... while resolution in cryo-electron microscopy is a frequency space comparison of two halves of the data, which strives to ... Frank, Joachim (2006). Three-Dimnsional Electron Microscopy of Macromolecular Assemblies. New York: Oxford University Press. ... Journal of Microscopy. 127 (2): 127-138. doi:10.1111/j.1365-2818.1982.tb00405.x. PMID 7120365. S2CID 27206060. Böttcher, B.; ...
Centrosome cycle
Chrétien D, Buendia B, Fuller SD, Karsenti E (November 1997). "Reconstruction of the centrosome cycle from cryoelectron ... "Centriole cycle in Chinese hamster ovary cells as determined by whole-mount electron microscopy". The Journal of Cell Biology. ...
2000s
RNA interference Neutrino insights Genome progress CMB structure and polarization Attosecond physics TRP channels Cryoelectron ... fossil fish Cloaking technology Macular degeneration patients Biodiversity and speciation Sub-diffraction-limit microscopy LTP ...
Metabolon
... and was observed using chemical cross-linking coupled to mass spectrometry and visualized by cryo-electron microscopy. The ...
Biological data visualization
... visualization of larger complexes by cryo-electron microscopy, and visualization of the location of proteins and complexes ... Plattner H, Hentschel J (2006). "Sub-Second Cellular Dynamics: Time-Resolved Electron Microscopy and Functional Correlation". A ... Genomic and assembly data Alignments, phylogeny and evolution Systems biology Molecular graphics Microscopy Magnetic resonance ... electron microscopy and cell and tissue imaging. In contrast, visualization of trajectories has long been a prominent part of ...
Presenilin
Structures have been solved of the assembled gamma secretase complex by cryo-electron microscopy, demonstrating significant ... as well as cryo-electron microscopy of the human gamma-secretase complex, indicate nine transmembrane helices. Presenilins are ...
Shlomo Rakib
"Representation theoretic patterns in three dimensional cryo-electron microscopy III - Presence of Point Symmetries." Gurevich, ...
Pyrenoid
When examined with transmission electron microscopy, the pyrenoid matrix appears as a roughly circular electron dense granular ... "Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography". eLife. 4: e04889. doi: ... visible using light microscopy. By contrast, in diatoms and dinoflagellates, there can be multiple pyrenoids. The Chlamydomonas ...
PSMD1
... provides an alternative structure of 19S base via an integrative approach combining data from cryoelectron microscopy, X-ray ...
Transformative High-Resolution Cryoelectron Microscopy (CryoEM)
The program aims to broaden access to high-resolution cryoelectron microscopy (cryoEM) for biomedical researchers, by creating ... Transformative High Resolution Cryo-Electron Microscopy (CryoEM). *Transformative Research to Address Health Disparities and ... Transformative High-Resolution Cryoelectron Microscopy Program. The program is broadening access to high-resolution ... Putting the freeze on COVID-19: Collecting images of SARS-CoV-2 using cryoelectron microscopy. Read highlight here! ...
The integrative role of cryo electron microscopy in molecular and cellular structural biology
... the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of ... Keywords: Crystallography; Structural biology; Super-resolution microscopy; cryo electron microscopy; cryo electron tomography ... The integrative role of cryo electron microscopy in molecular and cellular structural biology Biol Cell. 2017 Feb;109(2):81-93. ... the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of ...
Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image...
Using a synergistic application of cryo-electron microscopy, cryo-electron tomography, and three-dimensional reconstruction, we ... Kokkinopoulou et al.9, for example, applied transmission electron microscopy (TEM) and cryo-electron microscopy (cryo-EM) to ... Using a synergistic application of cryo-electron microscopy, cryo-electron tomography, and three-dimensional reconstruction, we ... Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image ...
Cryo-Electron Microscopy - EMBL Imaging Centre
The Cryo-Electron Microscopy Service Platform at EMBL Heidelberg is now offering remote service to all interested users, ... EMBL Heidelberg hosts a cryo-electron microscopy (cryo-EM) service platform, available for use by external scientists with both ... Open access to cutting-edge electron and light microscopy We provide researchers from Europe and beyond with a synergistic ... including the COVID-19-related projects (single-particle analysis and cryo-electron tomography). Due to the current situation, ...
Cryo-electron Microscopy Offers Atomic-Level Images of How Drug Binds to Cancer Protein
Researchers have produced atomic-level cryo-electron microscopy (cryo-EM) images detailing how a small molecule allosteric- ... Cryo-electron Microscopy Offers Atomic-Level Images of How Drug Binds to Cancer Protein. ... Researchers have produced atomic-level cryo-electron microscopy (cryo-EM) images detailing how a small molecule allosteric- ... Researchers have produced atomic-level cryo-electron microscopy (cryo-EM) images detailing how a small molecule allosteric- ...
AlbaNova | Computational Reconstruction in Cryo-Electron Microscopy
Novel AI-Based Method to Analyze Cryo-Electron Microscopy Data
Cryo-electron microscopy is a fairly new method used to examine the structure of materials, proteins or cells. Theoretically, ... But, cryo-electron microscopy offers only three-dimensional structures, and it cannot identify proteins to categorize their ... Novel AI-Based Method to Analyze Cryo-Electron Microscopy Data. *Download PDF Copy ... The researchers efficiently developed an AI-based approach for analyzing data from cryo-electron microscopy. In the coming days ...
K2 Summit camera enables scientists to break the 3 Å barrier in structural biology using cryo-electron microscopy (cryo-EM) |...
... high-resolution structures solved by single particle cryo-electron microscopy (cryo-EM) created speculation that 3 Å might be ... K2 Summit camera enables scientists to break the 3 Å barrier in structural biology using cryo-electron microscopy (cryo-EM). ... For many years the lack of sub 3 Å (angstrom) high-resolution structures solved by single particle cryo-electron microscopy ( ... Å resolution reconstruction of the Thermoplasma acidophilum 20 S proteasome using cryo electron microscopy, eLife 2015;10.7554/ ...
Cryo-electron microscopy reveals detailed blueprint of viral genome replication machinery - Master Doctor
Frontiers | Discovery of Exosomes From Tick Saliva and Salivary Glands Reveals Therapeutic Roles for CXCL12 and IL-8 in Wound...
Cryo-electron microscopy (cryo-EM) analysis revealed that tick saliva and salivary glands are composed of heterogeneous ... Cryo-Electron Microscopy. Cryo-electron microscopy (cryo-EM) was performed at two independent institutional facilities [ ... Cryo-Electron Microscopy Analysis Revealed a Novel Discovery of Exosomes in Tick Saliva and Salivary Glands. In this study, we ... A) Cryo-electron microscopy (Cryo-EM) images collected from two independent institutions [University of Texas Medical Branch ( ...
Plasmodium yoelii: influence of immune modulators on the development of the liver stage
Microscopic images and protein [IMAGE] | EurekAlert! Science News Releases
Research methods/Microscopy/Cryo electron microscopy * /Research methods/Microscopy/Electron microscopy/Transmission electron ... A new tool for cryo-electron microscopy. Forschungszentrum Juelich. Journal. Nature Methods. DOI. 10.1038/s41592-022-01586-0. ... www.fz-juelich.de/en/news/archive/notifications/2022/a-new-tool-for-cryo-electron-microscopy ...
Cryoelectron microscopy of mouse mammary tumor virus :: MPG.PuRe
Briggs, J. A. G., Watson, B. E., Gowen, B. E., & Fuller, S. D. (2004). Cryoelectron microscopy of mouse mammary tumor virus. ... Cryoelectron microscopy of Mouse mammary tumor virus, a Betaretrovirus, provided information about glycoprotein structure and ... Cryoelectron microscopy of mouse mammary tumor virus ...
Helmholtz Imaging Modalities - Related facilities to Cryo Electron Microscopy
Cryo-Electron Microscopy Study of Vesicles from Various Species - RICCEM
Advanced Center For Cryo-Electron Microscopy Facility - IISc - Bangalore India.
The Advanced Center for Cryo-Electron Microscopy Facility (ACCEM-IISc) at IISc Bengaluru is a world class cryo-EM facility in ... Copyright: Advanced Center For Cryo-Electron Microscopy Facility - IISc Bangalore. Powered by Surfzone Technologies ... scientists all over the country to implement single particle cryo-EM and cryo-electron tomography (cryo-ET) for high-resolution ...
Muscle cells need calcium ions | Max-Planck-Gesellschaft
Cryo electron microscopy of SARS-CoV-2 spike in prefusion state
Hyderabad: Cryo- electron microscopy ushered in at CSIR-CCMB - Telangana Tribune
Cryo- electron microscopy ushered in at CSIR-CCMB. Hyderabad: An innovative center for cryo-electron microscopy was ushered in ... Hyderabad: Cryo- electron microscopy ushered in at CSIR-CCMB. by Telangana Tribune March 25, 2022 24 0 Comment 3 min read ... "The modern cryo-electron microscopy facility is expected to help us view the functioning of several molecular machines that ... Hyderabad is the 2nd city in the country to organize a contemporary cryo-electron microscopy center. Such a center enables ...
Items where Subject is "Cryo-electron microscopy" - CSHL Scientific Digital Repository
A national facility for biological cryo-electron microscopy. - CAMS Oxford Institute
Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated ... Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an ... three-dimensional electron microscopy, Academies and Institutes, Automation, Cryoelectron Microscopy, Crystallography, United ... Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. It is now able to provide an ...
Research News: U-Wisconsin Adding Cryo-Electron Microscopy to Imaging Arsenal
How cryo-electron microscopy differs from other imaging techniques?. Cryo-EM gives researchers an extremely detailed view of a ... Research News: U-Wisconsin Adding Cryo-Electron Microscopy to Imaging Arsenal Posted by Rebecca Inch-Partridge on Tue, Feb 13, ... Now UW-Madison is adding another piece of equipment to their state-of-the-art imaging arsenal: a cryo-electron microscopy (cryo ... The BOC provides state-of-the-art instrumentation for light and fluorescence based microscopy, including epifluorescence, ...
Omicron's Spike Protein Captured via Cryo-Electron Microscopy | Natural healing and nutrition
Researchers use cryo-electron microscopy to resolve 3D structure of protein nanoparticle
... *Download PDF Copy ... Tags: Compound, Drug Delivery, E. coli, Electron, Electron Microscopy, Helix, Microscopy, Molecule, Nanoparticle, Nanoparticles ... A large amount of TIP60 was expressed in E. coli, and a purified sample was observed at the cryo-electron microscope facility ... research group solved the detailed three-dimensional structure of the TIP60 using single-particle cryo-electron microscopy. ...
Multipartners communication between organelles deciphered by cryo-electron microscopy Daniel Levy PCC Paris
Multipartners communication between organelles deciphered by cryo-electron microscopy Doc envoyé stage_M2-Dlevy-Curie-MCS.pdf ... In vitro and in cellulo characterization of VapB-Microtubules interaction by Cryo Electron Microscopy and optical microscopies ... Multipartners communication between organelles deciphered by cryo-electron microscopy _ Daniel Levy _ PCC _ Paris. Home / stage ... 3D architecture at sub-nanometric resolution of BLOC-1 endosomal complex by cryo-electron microscopy _ Daniel Levy _ PCC _ ...
SARS-CoV-2 neutralizing antibody structures inform therapeutic strategies - CaltechAUTHORS
Antibodies; Cryoelectron microscopy; SARS-CoV-2. Issue or Number:. 7839. PubMed Central ID:. PMC8092461. ... Electron microscopy was performed in the Caltech Beckman Institute Resource Center for Transmission Electron Microscopy with ... C135-S 2P and C144-S 6P complexes have been deposited at the PDB and the Electron Microscopy Data Bank (EMDB) under the ...
Resolving the Morphology of Peptoid Vesicles at the 1 nm Length Scale Using Cryogenic Electron Microscopy (Journal Article) |...
Cryo-electron microscopy of artificial biological membranes journal, November 1985 * Lepault, Jean; Pattus, Franc; Martin, ... MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy journal, February 2017 * Zheng ... A new method to measure bilayer thickness: Cryo-electron microscopy of frozen hydrated liposomes and image simulation journal, ... Electron microscopy of the stacked disk aggregate of tobacco mosaic virus protein journal, August 1974 * Unwin, P. N. T.; Klug ...
Drug Resistance - GEN - Genetic Engineering and Biotechnology News
ElectronCrystallographyTomographySpectroscopyResearchersCharacterizationCentersConfocalFluorescenceEscherichiaReconstructionEnablesStructureResearchScientistsTransmission electron mCRYOEMSingle-particle cryo-electron m2020ProteinsCorrelativeCryogenicMacromolecularStructuresFacilityCryo-electron microscoTechnique called cryo-electron mLight microscopyReconstructionsProteinStructuralBiomoleculesHelicalLife sciencesAtomic resolutionComputationalImageHigh-resolutionReceptor2017BiochemistryElectronsDataRibosomeResolutionMoleculesAdvancesNegative stainPreparation
Electron56
- After gradually moving away from preparation methods prone to artefacts such as plastic embedding and negative staining for cell sections and single particles, the field of cryo electron microscopy (cryo-EM) is now heading off at unprecedented speed towards high-resolution analysis of biological objects of various sizes. (nih.gov)
- Using a synergistic application of cryo-electron microscopy, cryo-electron tomography, and three-dimensional reconstruction, we revealed the unique morphological details of the biomolecules and their distribution/association with the surface of polystyrene NPs at a nanoscale resolution. (nature.com)
- Other techniques to characterize the BC, such as gel electrophoresis, differential centrifugal sedimentation, and conventional electron microscopy, also fail to provide structural details. (nature.com)
- The objective of the current study is to investigate the morphological details of the biomolecules and their distribution and association with the surface of carboxylated polystyrene NPs (PS-COOH NPs) at the nanometer scale using high-resolution transmission electron microscopy (HRTEM), cryo-EM, cryo-electron tomography (cryo-ET), and image analysis. (nature.com)
- EMBL Heidelberg hosts a cryo-electron microscopy (cryo-EM) service platform, available for use by external scientists with both single particle and tomography projects. (embl.org)
- The Cryo-Electron Microscopy Service Platform at EMBL Heidelberg is now offering remote service to all interested users, including the COVID-19-related projects (single-particle analysis and cryo-electron tomography). (embl.org)
- Researchers have produced atomic-level cryo-electron microscopy (cryo-EM) images detailing how a small molecule allosteric-inhibiting drug binds to p97, a cancer cell protein, they reported in the journal Science. (cancernetwork.com)
- The researchers efficiently developed an AI-based approach for analyzing data from cryo-electron microscopy. (azorobotics.com)
- Cryo-electron microscopy is a fairly new method used to examine the structure of materials, proteins or cells. (azorobotics.com)
- But, cryo-electron microscopy offers only three-dimensional structures, and it cannot identify proteins to categorize their identity. (azorobotics.com)
- For many years the lack of sub 3 Å (angstrom) high-resolution structures solved by single particle cryo-electron microscopy (cryo-EM) created speculation that 3 Å might be the fundamental resolution limit of the technique. (gatan.com)
- The research article 2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20 S proteasome using cryo electron microscopy , eLife 2015;10.7554/eLife.06380, demonstrates that with the help of the K2 Summit camera, single-particle cryo-EM can compete with x-ray crystallography to determine protein structures for rational drug design at 2.8 Å resolution. (gatan.com)
- Cryo-electron microscopy (cryo-EM) analysis revealed that tick saliva and salivary glands are composed of heterogeneous populations of in vivo exosomes with sizes ranging from 30 to 200 nm. (frontiersin.org)
- The Advanced Center for Cryo-Electron Microscopy Facility (ACCEM-IISc) at IISc Bengaluru is a world class cryo-EM facility in India, which provides access to the researchers & scientists all over the country to implement single particle cryo-EM and cryo-electron tomography (cryo-ET) for high-resolution imaging and structural characterization of biological macromolecules. (iisc.ac.in)
- An innovative center for cryo-electron microscopy was ushered in by Dr Shekhar Mande, Director-General, Council of Scientific and also Industrial Research (CSIR), at the Centre for Cellular and also Molecular Biology (CCMB) on Friday. (telanganatribune.com)
- A launch claimed, with this center, Hyderabad is the 2nd city in the country to organize a contemporary cryo-electron microscopy center. (telanganatribune.com)
- The modern cryo-electron microscopy facility is expected to help us view the functioning of several molecular machines that operate in the cell that was earlier not amenable to conventional structure determination methods such as X-ray crystallography or Nuclear Magnetic Resonance (NMR)," claimed Dr Rajan Sankaranarayanan, a noteworthy architectural biologist at CCMB. (telanganatribune.com)
- The chasm between structural and cellular biology is diminishing, and this will allow addressing some of the very fundamental and exciting problems of biology with techniques like cryo-electron microscopy," claimed Dr Shekhar Mande, Director-General, CSIR. (telanganatribune.com)
- A national facility for biological cryo-electron microscopy. (ox.ac.uk)
- Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. (ox.ac.uk)
- Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. (ox.ac.uk)
- Now UW-Madison is adding another piece of equipment to their state-of-the-art imaging arsenal: a cryo-electron microscopy (cryo-EM) facility. (biotech-calendar.com)
- How cryo-electron microscopy differs from other imaging techniques? (biotech-calendar.com)
- The near-atomic resolution analysis-using cryo-electron microscopy-yields insights into how the heavily mutated Omicron variant attaches to and infects human cells. (sciencelock.in)
- In the present study, the joint research group solved the detailed three-dimensional structure of the TIP60 using single-particle cryo-electron microscopy. (azolifesciences.com)
- In vitro and in cellulo characterization of VapB-Microtubules interaction by Cryo Electron Microscopy and optical microscopies. (master-physique-biologie.fr)
- A new approach for characterizing the vesicle membrane morphology based on low-dose cryogenic electron microscopy (cryo-EM) is described. (osti.gov)
- Candidates using cryo-electron microscopy techniques to explore cutting-edge questions of structure and function are encouraged to apply. (cell.com)
- Structural transitions in the GTP cap visualized by cryo-electron microscopy of catalytically inactive microtubules. (icr.ac.uk)
- Here, we use cryo-electron microscopy and total internal reflection fluorescence microscopy of GTP hydrolysis-deficient MTs assembled from mutant recombinant human tubulin to investigate the structure of a GTP-bound MT lattice. (icr.ac.uk)
- In situ transmission electron microscopy (TEM) is one of the most powerful approaches for revealing physical and chemical process dynamics at atomic resolutions. (semanticscholar.org)
- Direct Observation of Oxygen Evolution and Surface Restructuring on Mn2O3 Nanocatalysts Using In Situ and Ex Situ Transmission Electron Microscopy. (semanticscholar.org)
- Transmission electron microscopy (TEM) has long been used as the ultimate characterization technique for nanomaterials at the atomic level. (semanticscholar.org)
- Using cryogenic-electron microscopy, we present a model of RyR1 with a 2.45-Å resolution before local refinement, revealing a binding site in the RY1&2 domain (3.10 Å local resolution), where the Rycal ARM210 binds cooperatively with ATP and stabilizes the closed state of RyR1. (cell.com)
- ATP-Bound States of Groel Captured by Cryo-Electron Microscopy. (expasy.org)
- It was obtained through cryo-electron microscopy. (scienceblogs.com)
- The resolution isn't great, because electron microscopy can only do so much, so the structure model is built from the alpha carbon backbones of these 420 proteins. (scienceblogs.com)
- Analytical Cryo-Scanning Electron Microscopy of Hydrated Polymers and Microgels. (stevens.edu)
- Jason has recently joined the Industrial Liaison Office (May 2017) as a cryo-electron microscopy scientist where he will be supporting industry clients seeking advanced imaging capabilities of eBIC. (diamond.ac.uk)
- for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution. (nobelprize.org)
- It provides users with access to a powerful and synergistic portfolio in light and electron microscopy technologies and correlative approaches, including academically developed methods not yet commercially available. (embl.org)
- Users showed particularly strong interest in cryo-electron and super-resolution microscopy technologies, with samples covering different mammalian species, but also plants, bacteria and marine organisms," she said. (embl.org)
- An international team of researchers at OIST and University of Otago have used the Nobel-winning cryo-electron microscopy method to reconstruct t. (oist.jp)
- Using a combination of femtosecond spectroscopy with electron microscopy techniques, the flow of electrons on femtosecond timescales was tracked. (oist.jp)
- The structures were created using the cryo-electron microscopy method, by freezing infectious BKV particles and taking thousands of images using the microscopes. (wordpress.com)
- Professor Neil Ranson, Director of Cryo-Electron Microscopy at the University of Leeds, said: "Cryo-electron microscopy has been around for 30 years and has been incredibly useful, but until recently the technology lacked the ability to routinely look at molecules at the level of detail needed. (wordpress.com)
- We used newly developed techniques for semiautomated reconstruction from serial electron microscopy (EM) [14] to obtain the shapes of neurons, the location of synapses, and the resultant synaptic connectivity. (janelia.org)
- The challenge of recovering the topology of massive neuronal circuits can potentially be met by high throughput Electron Microscopy (EM) imagery. (janelia.org)
- Semi-automated reconstruction of neural circuits using electron microscopy. (janelia.org)
- Previously used to reconstruct the C. elegans wiring diagram, serial-section transmission electron microscopy (ssTEM) is a proven technique for the task. (janelia.org)
- We review progress in the processing of electron microscopy images and, in particular, a semi-automated reconstruction pipeline deployed at Janelia. (janelia.org)
- Increasing depth resolution of electron microscopy of neural circuits using sparse tomographic reconstruction. (janelia.org)
- Jan. 10, 2020-D. Borden Lacy and colleagues used cryo-electron microscopy to define the structure of a C. diff toxin, providing a framework for the design of novel therapeutics. (vumc.org)
- Vicki is visiting us from the newly established Living Systems Institute (LSI) Exeter, where she and her group research protein transport across membranes, with a strong methodological focus on cryo-electron microscopy. (cam.ac.uk)
- JEOL USA awarded two Grand Prizes to winners of its 2020 Electron Microscopy Image Contest, and kicked off its 2021 Image Contest at the beginning of the new year. (labbulletin.com)
- Lita explains that "Manipulating Drosophila melanogaster is a bit challenging from an electron microscopy point of view, but is so indispensable for genomic research in Amyotrophic lateral sclerosis (ALS), Alzheimer's, and other debilitating diseases. (labbulletin.com)
Crystallography1
- Cryoelectron microscopy and x-ray crystallography analyses of NoV VLPs identified the shell (S) and protruding domains (subdomains P1-1, P1-2, and P2) ( 18 ). (cdc.gov)
Tomography1
- The program is broadening access to high-resolution cryoelectron microscopy (cryoEM) and tomography (cryoET) for biomedical researchers by creating national service centers, and cultivating a skilled workforce through the development and implementation of cryoEM tra ining material . (nih.gov)
Spectroscopy1
- This, along with the confocal microscopy, NMR spectroscopy and also X-ray diffraction centers at CCMB, makes it an awesome center for scientists to consider information of living cells like never ever previously. (telanganatribune.com)
Researchers2
- We provide researchers from Europe and beyond with a synergistic portfolio of imaging services including cryo-EM, super-resolution and intravital microscopy to enable new ground-breaking research that crosses the scales of biology. (embl.org)
- To offer researchers from academia and industry rapid access to the most advanced microscopy technologies available, in recent months the EMBL Imaging Centre staff have undertaken testing and equipment commissioning including internal and external user trials. (embl.org)
Characterization1
- Microscopy 101: Scanning probes or scanning electrons: a practical guide to select a method for nanoscale characterization. (stevens.edu)
Centers1
- Did you miss one of the free monthly webinars on cryoEM current practices and strategies hosted by the National Centers for Cryoelectron Microscopy? (nih.gov)
Confocal1
- The BOC provides state-of-the-art instrumentation for light and fluorescence based microscopy, including epifluorescence, confocal, and super-resolution imaging. (biotech-calendar.com)
Fluorescence1
- Single molecule fluorescence microscopy has the unique advantage to provide real-time information on the spatiotemporal assembly of individual protein complexes in cellular membranes. (bvsalud.org)
Escherichia1
- Cryoelectron-microscopy framework of the enteropathogenic Escherichia coli variety Three secretion method EspA filament. (socialmediastore.net)
Reconstruction1
- Finally, I will describe some very recent work where we try to combine experimental and theoretical methods to study ensembles of molecular conformations, and how we would like to use these types of reconstruction methods for wider classes of experimental data, including e.g. neutron scattering and light microscopy. (albanova.se)
Enables1
- For proteins, the microscopy enables highly elaborate images of the structure to be created at the level of single-atom. (azorobotics.com)
Structure1
- Cryoelectron microscopy of Mouse mammary tumor virus, a Betaretrovirus, provided information about glycoprotein structure and core formation. (mpg.de)
Research1
- A form of microscopy is shaking up nanoscience research and forcing scientists to reconsider many established theories. (chemistryworld.com)
Scientists1
- Microscopy is an important tool for scientists in the study of cells, tissues, and organs. (nih.gov)
Transmission electron m8
- The calculated structures are cross-validated using an independent data set of 691 ssNMR constraints and scanning transmission electron microscopy measurements. (mpg.de)
- Transmission electron microscopy and cryo electron microscopy analysis of standard myelinosome preparation from TM4 Sertoli cells and human seminal plasma samples. (archives-ouvertes.fr)
- The technological advances of the 1940s allowed the observation of cellular organelles embedded in heavy metal stains by transmission electron microscopy (TEM). (nih.gov)
- Transmission electron microscopy (TEM) is an essential method in virology because it allows for direct visualization of virus morphology at a nanometer scale. (nature.com)
- Observing viral morphology is essential in virology, for which transmission electron microscopy (TEM) is the most widely used technique because it allows direct visualization at the nanometer scale. (nature.com)
- d ) Transmission electron microscopy (TEM) of heavy-metal-coated virions produces ( e ) a reverse-contrast image of the virus. (nature.com)
- Mineralization in the MVs will be visualized by cryo-transmission electron microscopy with a temporal resolution to decipher the pathway by which mineral is formed in the MVs. (fapesp.br)
- Volta potential phase plate for in-focus phase contrast transmission electron microscopy. (mpg.de)
CRYOEM11
- The Cryo-Electron Microscopy (CRYOEM) core provides the NIDDK scientific community with equipment and training in preparing and imaging negative stain and cryo-EM grids. (nih.gov)
- The program is broadening access to high-resolution cryoelectron microscopy (cryoEM) and tomography (cryoET) for biomedical researchers by creating national service centers, and cultivating a skilled workforce through the development and implementation of cryoEM tra ining material . (nih.gov)
- Did you miss one of the free monthly webinars on cryoEM current practices and strategies hosted by the National Centers for Cryoelectron Microscopy? (nih.gov)
- This NIH Common Fund initiative will establish national service centers to increase research capacity for molecular structure determination by high resolution cryoelectron microscopy (cryoEM). (nih.gov)
- Cryoelectron microscopy (cryoEM) has been selected for Common Fund support because it opens up previously inaccessible levels of biological organization to high resolution structure analysis, and will vastly extend the range of functions and interactions that can be targeted for basic research and structure-based development of therapeutics. (nih.gov)
- An emerging related cryoEM method, cryoelectron tomography (cryoET), can image macromolecular assemblies in intact unstained frozen cells in 3-D. Together, these methods enable high-resolution analysis of previously inaccessible levels of biological organization, ranging from below 100kD to whole cells. (nih.gov)
- A. The purpose of this funding opportunity announcement (FOA) is to provide regional access to state-of-the-art data collection capabilities to cryoelectron microscopy (cryoEM) laboratories. (nih.gov)
- Development of cryogenic electron microscopy (cryoEM) in the 1970s provided the means to image these macromolecules while keeping them hydrated in biochemically functional buffer conditions by instantaneously freezing them at −180°C. However, the resolution attainable for these samples through cryoEM was limited. (nih.gov)
- The lab uses biochemistry, cryo electron microscopy (cryoEM), and other tools to attack the problems, while also working on method development to expand applications of cryoEM on life sciences research. (nih.gov)
- We use cryo electron microscopy (cryoEM) as a primary tool to study the structures of membrane proteins. (nih.gov)
- Given these challenges, we envision that application of the recently reported cryo-electron microscopy (CryoEM) modality micro-crystal electron diffraction (MicroED) could lead to vertical advances in the field of NP discovery directly responsive to this NOSI, as MicroED has recently been demonstrated to provide unambiguous structures from sub-micron-sized crystals of structurally complex chemical compounds that had failed to yield large crystals suitable for X-ray analysis. (nih.gov)
Single-particle cryo-electron m3
- To further investigate the relationships between BtAdVs and CAdVs, we conducted mass spectrometric analysis and single-particle cryo-electron microscopy reconstructions of the BtAdV 250-A capsid and also analyzed the in vitro host ranges of both viruses. (elsevier.com)
- Together, these results provide insights into the reductive nature of microsporidian evolution and unravel a novel mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. (imex-revista.com)
- The conserved theme of ribosome dimerization revealed by single-particle cryo-electron microscopy. (imex-revista.com)
20201
- 2020) Cryo-Electron Microscopy of Endogenous Yeast Exosomes. (cls.edu.cn)
Proteins9
- Electron microscopy, in ever-more-incremental strokes, has revealed this world - without it, we would not be able to see viruses or proteins, keys to understanding the processes of life. (freethink.com)
- IRP researchers led by Sriram Subramaniam, Ph.D. , developed groundbreaking new technology based on cryo-electron microscopy (cryo-EM) to determine the atomic structures of proteins. (nih.gov)
- NIH funded the development and dissemination of cryo-electron microscopy (cryo-EM), a tool that enables high-resolution images of proteins and other biological structures. (nih.gov)
- Using cryo-electron microscopy, they have uncovered the mechanisms by which some HAT proteins transport certain amino acids and not others. (cnio.es)
- Thanks to the latest high-resolution structural technologies such as cryo-electron microscopy, combined with computational modelling and the design of mutants of these proteins, the researchers have been able to observe the structure of one of the members of this protein family in atomic detail and decipher its function. (cnio.es)
- Thanks to cryo-electron microscopy, a field in which Llorca is an international expert, visualisation of the molecular structure of proteins has taken a giant step towards what we now know as the golden age of 3D structures. (cnio.es)
- The researchers combined x-ray crystallographic data, which gives 3-D atomic details of the constituent viral proteins, with cryo-electron microscopy images to determine how proteins in the T4 phage rearrange themselves during cell infection. (infectioncontroltoday.com)
- NINDS Cryo-Electron Microscopy core surves many structural biology labs that use Cryo-EM single particle analysis to study the structures of proteins & macromolecules. (nih.gov)
- High-resolution cryo-electron microscopy determines the atomic-scale structure of proteins and protein complexes to uncover the molecular mechanisms regulating cellular processes. (berstructuralbioportal.org)
Correlative4
- The facility's expert team supports and collaborates on a large number of bio EM techniques ranging from standard SEM and TEM to immuno EM, correlative light and electron microscopy, cryo tomography and single particle analysis. (monash.edu)
- Towards correlative super-resolution fluorescence and electron cryo-microscopy. (nih.gov)
- Photonic-chip assisted correlative light and electron microscopy. (embl.org)
- The CBS is offering a group leader position in cell structural biology for a specialist in cryo-electron microscopy, cryo-tomography and correlative a. (cnrs.fr)
Cryogenic3
- Modified cryogenic electron microscopy can analyze viral structures better than ever before. (the-scientist.com)
- In this project, we propose the use of state-of-art cryogenic electron microscopy techniques to track mineral formation in MVs and their interactions within the extracellular matrix. (fapesp.br)
- Next, the team isolated chlorosomes from the mutant and the wild-type forms of the bacteria and used cryo-electron microscopy -- a type of electron microscopy that is performed at super-cold cryogenic temperatures -- to take pictures of the chlorosomes. (azooptics.com)
Macromolecular3
- Cryo-electron microscopy (cryo-EM), combined with image processing, is an increasingly powerful tool for structure determination of macromolecular protein complexes and assemblies. (ox.ac.uk)
- Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions. (imex-revista.com)
- Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions. (gotonextstep.com)
Structures6
- One method applied for that is cryo electron microscopy (cryo-EM), which can be used to make three-dimensional structures of biomolecules visible. (mpg.de)
- Typically, it is believed that in order to solve structures of biological macromolecules at atomic resolution with cryo-electron microscopy (cryo-EM), a technique for which the 2017 Nobel Prize in chemistry was awarded, a high power microscope like the Krios must be employed. (yalescientific.org)
- This year's prize went to the development of cryo-electron microscopy, a technique that allows the structures of biomolecules to be revealed where other techniques fail. (compoundchem.com)
- Cryo-electron microscopy (Cryo-EM) analysis of the yeast 60S-RQC structures revealed that Rqc2 occupies a large proportion of the inter-subunit surface of 60S and prevents 40S-subunit reassociation after 80S splitting 10 , 12 , 29 . (nature.com)
- used cryo-electron microscopy to look at structures of the M. tuberculosis RNA polymerase in different states, including when it was bound to fidaxomicin. (elifesciences.org)
- Here, we report cryo-electron microscopy structures of Escherichia coli transcription-translation complexes (TTCs) containing different-length mRNA spacers between RNAP and the ribosome active-center P site. (cheric.org)
Facility9
- The Monash Ramaciotti Centre is a leading facility for life sciences electron microscopy. (monash.edu)
- In addition to the confocal microscopy, NMR spectroscopy and X-ray diffraction facilities at CCMB, the addition of cryo-electron microscopy makes it a remarkable facility for researchers to investigate details of living cells like never before. (pharmiweb.com)
- Here, a brief overview is provided of the plans for a UK national three-dimensional electron-microscopy facility for integrated structural biology to enable internationally leading research on the machinery of life. (ox.ac.uk)
- LISCB hosts a Midlands Regional cryo-Electron Microscopy (cryo-EM) Facility. (le.ac.uk)
- The facility provides advanced expertise in electron microscopy, from sample preparation to image analysis, for a wide variety of biological samples. (embl.org)
- UT Southwestern's Cryo-Electron Microscopy Facility is supported by a grant from the Cancer Prevention & Research Institute of Texas. (utsouthwestern.edu)
- The scientists' latest accomplishment hinged on the use of one of the highest-resolution transmission electron microscopes in the world, called TEAM I. It's located at the National Center for Electron Microscopy, which is a Molecular Foundry facility. (nextbigfuture.com)
- Core Facility for Electron Microscopy, and all members of the resulting refined model and half map 2 (SSU-body focused) and EMD-11437-additional map 2. (thecoinradar.com)
- The facility will house cryo-electron microscopy, powerful visualising equipment allowing researchers to examine diseases down to the finest detail, or the molecular and even atomic level. (edu.au)
Cryo-electron microsco1
- Learning from terabytes of images, AI diagnostics can match clinicians in detecting pathologies in medical scan and X-ray data, while cryo-electron microscopy images can reveal 3D molecular structure within cells and viruses much faster than traditional X-ray diffraction techniques. (computerweekly.com)
Technique called cryo-electron m2
- They used a technique called cryo-electron microscopy. (nih.gov)
- To learn how these antibodies neutralize ebolaviruses, LJI Postdoctoral Fellows Xiaoying Yu, Ph.D., and Jake Milligan, Ph.D., spearheaded the use of an imaging technique called cryo-electron microscopy. (scitechdaily.com)
Light microscopy2
- Because the wavelength of electrons [6] is so much shorter than light [7] , much finer detail can be obtained using electron microscopy than with light microscopy. (peterjamesthomas.com)
- History of the electron microscope, spanning from the origins of light microscopy to modern techniques such as cryo-electron microscopy. (thermofisher.com)
Reconstructions2
- The improved resolution allowed for model http://www.pafiry.net/cheap-nitrostat-canada/ building and refinement into electron cryo-microscopy reconstructions. (gotonextstep.com)
- Although tropomyosin is well defined in electron microscopy helical reconstructions of thin filaments, troponin density is mostly lost. (infona.pl)
Protein8
- We used the methods of cryo-electron microscopy with bioinformatics, protein design and expression, biochemistry and immunology for the development of a novel vaccine nanoplatform. (nih.gov)
- But now, armed with a suite of new technology, cryo-electron microscopy has revealed the individual atoms in a protein - and how they really fit together. (freethink.com)
- Both teams imaged apoferritin, a protein, Callaway reports, whose Steelix -like strength and stability makes it a popular cryo-electron microscopy subject. (freethink.com)
- An image of the protein apoferritin created with cryo-electron microscopy. (freethink.com)
- Cryo-electron microscopy (cryo-EM) data define the overall envelope of the assembly and rigid-body orientation of the subunits while solid-state nuclear magnetic resonance (ssNMR) chemical shifts and distance constraints define the local secondary structure, protein fold and inter-subunit interactions. (mpg.de)
- Here, we introduce the nanoparticle-aided cryo-electron microscopy sampling (NACS) method to access the conformational distribution of a protein molecule. (ibs.re.kr)
- By analyzing the chimpanzee adenovirus used by AstraZeneca with cryo-electron microscopy, which allows for molecular-level details to be seen, researchers found that the adenovirus attracts the blood protein known as "platelet factor 4" to it. (ctvnews.ca)
- Structure of the LAT2/CD98hc protein, resolved by cryo-electron microscopy. (cnio.es)
Structural10
- Congratulations to Jacques Dubochet, Joachim Frank, and Richard Henderson, who have received the 2017 Nobel Prize in Chemistry for developing cryo-electron microscopy, a technique that allows scientists to visualize the structural details of biological molecules and gain insights into how living systems function. (emdataresource.org)
- Three-dimensional electron microscopy is an enormously powerful tool for structural biologists. (ox.ac.uk)
- Here, we propose a structural model of the yeast pontin/reptin complex based on a cryo-electron microscopy reconstruction at 13 A. Pontin/reptin hetero-dodecamers were purified from in vivo assembled complexes forming a double ring. (nih.gov)
- Two nanogold particles are labeled at two target sites, and the interparticle distance is measured as a structural parameter via cryo-electron microscopy (cryo-EM). (ibs.re.kr)
- The Rad51-DNA filaments are amenable to structural studies using cryo-electron microscopy (cryo-EM). (uthscsa.edu)
- The SSCB utilizes advanced microscopy methods and cellular biology approaches to elucidate the molecular and structural basis of mechanisms underlying normal and disordered hearing and balance. (nih.gov)
- Training in structural cell biology and advanced microscopy imaging. (nih.gov)
- Structural analysis of the C. elegans dauer larval anterior sensilla by focused ion beam-scanning electron microscopy. (embl.org)
- The combination of structural resolution by cryo-electron microscopy with molecular dynamics calculations and functional studies provides an experimental platform with a lot of potential that allows us to unravel the function of amino acid transporters. (cnio.es)
- To identify the structural determinants of Fdx binding to RNAP, we determined the 3.4 Å cryo-electron microscopy structure of a complete M. tuberculosis RNAP holoenzyme in complex with Fdx. (elifesciences.org)
Biomolecules2
- The Nobel Prize in Chemistry 2017 is awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for the development of cryo-electron microscopy, which both simplifies and improves the imaging of biomolecules. (peterjamesthomas.com)
- Cryo-electron microscopy, which both simplifies and improves the imaging of biomolecules. (tutorialspoint.com)
Helical2
- Structure of HIV-1 capsid assemblies by cryo-electron microscopy and iterative helical real-space reconstruction. (ox.ac.uk)
- Here, we describe purification of full-length recombinant metastable helical nucleocapsid of Hantaan virus ( Hantaviridae family, Bunyavirales order) and determine its structure at 3.3 Å resolution by cryo-electron microscopy. (elifesciences.org)
Life sciences1
- We've created the life sciences learning center to connect scientists around the world to the latest information on electron microscopy technology and to guide you to the best solution that will accelerate your next discovery or breakthrough. (thermofisher.com)
Atomic resolution1
- Research teams at Germany's Max Planck Institute for Biophysical Chemistry and the Medical Research Council Laboratory of Molecular Biology in the U.K. have both recently pushed cryo-electron microscopy into atomic resolution. (freethink.com)
Computational1
- Almost one-half of IRP labs use Biowulf to process and analyze their research data in areas such as genomics, computational chemistry, and cryo-electron microscopy imaging. (nih.gov)
Image3
- We have determined the threedimensional structure of an amyloid fibril, formed by the SH3 domain of phosphatidylinositol-3′-kinase, using cryo-electron microscopy and image processing at 25 A resolution. (pasteur.fr)
- The cryo-electron microscopy image of the chimpanzee adenovirus which delivers the Oxford-AstraZeneca vaccine. (ctvnews.ca)
- EMAN2: an extensible image where can i buy cefzil processing suite for electron microscopy. (gisci.it)
High-resolution6
- Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. (nih.gov)
- Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology. (jic.ac.uk)
- For more details, see the Request for Information (RFI): Screening for High Resolution Cryo-Electron Microscopy ( NOT-RM-21-012 ). (nih.gov)
- High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy. (mpg.de)
- These properties make the single and dual layer graphene films ideal for high resolution TEM imaging, nanoparticles imaging and cryo-electron microscopy. (ravescientific.com)
- High-Resolution Cryoelectron Microscopy Structure of the Cyclic Nucleotide-Modulated Potassium Channel MloK1 in a Lipid Bilayer. (cornell.edu)
Receptor2
- When trying their cryo-electron microscopy techniques on a simplified version of the GABAA receptor - target of anxiety meds and anesthetics, among other things - they saw details that had never been seen before, including water molecules at a site for histamine, a driving factor in allergic reactions. (freethink.com)
- 카이스트(KAIST, 총장 이광형)는 생명과학과 송지준 교수 연구팀이 초저온 전자 현미경(cryo-Electron Microscopy)을 이용해 호르몬 조절 물질인 소마토스타틴(somatostatin)과 그 수용체인 소마토스타틴 리셉터 2(Somatostatin Receptor 2, SSTR2) 복합체의 3차원 원자 해상도 구조를 규명했다고 6일 발표했다. (kaist.ac.kr)
20171
- Cryo-electron microscopy is a relatively new and revolutionary technology (according to Nature) that was awarded a Nobel Prize in 2017. (surf.nl)
Biochemistry1
- The University of Wisconsin-Madison Department of Biochemistry will host the grand opening of its Cryo-Electron Microscopy Research Center (CEMRC) and Midwest Center for Cryo-Electron Tomography (MCCET) at 8:30 a.m. on June 7. (wisc.edu)
Electrons2
- The "cryo" part of cryo-electron microscopy refers to the flash-freezing of the sample before you riddle it with electrons. (freethink.com)
- Cryo-electron microscopy involves flash-freezing the study subject and then bombarding them with electrons to produce microscope images of individual molecules. (ctvnews.ca)
Data3
- Singer is "being recognized for foundational contributions to mathematical data analysis and the mathematics of cryo-electron microscopy. (princeton.edu)
- We were extremely fortunate to have extraordinarily talented scientists such as Petr Leiman and Victor Kostyuchenko as well as equally talented participation of Paul Chipman who did all the electron microscopy data collection, Rossmann added. (infectioncontroltoday.com)
- Meet FAST-EM, a system that creates a fundamental shift in electron microscopy, where unprecedented large areas can be imaged at the nanoscale while freeing up time for operators to focus on data analysis and interpretation. (delmic.com)
Ribosome1
- Cryo-electron microscopy analysis reveals that Hel2-bound ribosome are dominantly the rotated form with hybrid tRNAs. (nature.com)
Resolution4
- Holger Stark from the Max Planck Institute for Biophysical Chemistry in Göttingen and his team have broken a crucial resolution barrier in cryo electron microscopy. (mpg.de)
- The resolution revolution in cryo-electron microscopy]. (nih.gov)
- At Leicester, we are developing cutting-edge super-resolution localisation microscopy. (le.ac.uk)
- We have now determined the structure of these tubes at medium resolution (22 Å) by electron microscopy. (labex-gral.fr)
Molecules3
- Using cryo-electron microscopy, the spatial structure and function of individual molecules can be studied very precisely. (computerweekly.com)
- For more on cryo-EM see the blog post Cryo-Electron Microscopy Reveals Molecules in Ever Greater Detail . (nih.gov)
- They used genetic techniques to create a mutant bacterium with a more regular internal structure, cryo-electron microscopy to identify the larger distance constraints for the chlorosome, solid-state nuclear magnetic resonance (NMR) spectroscopy to determine the structure of the chlorosome's component chlorophyll molecules, and modeling to bring together all of the pieces and create a final picture of the chlorosome. (azooptics.com)
Advances1
- Recent advances in electron cryo microscopy have revolutionized how we address fundamental questions in cell and molecular biology. (snf.ch)
Negative stain1
- We have studied the shape of myosin VI, the actin minus-end directed motor, by negative stain and metal shadow electron microscopy. (infona.pl)
Preparation2
- Expertise in sample preparation for cryo-electron microscopy and cryo-electron tomography. (nih.gov)
- The described methods are useful for sample preparation in crystallographic analysis and other structure determination techniques, such as cryo-electron microscopy. (portlandpress.com)