Cross Reactions: Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.Immune Sera: Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Red Cross: International collective of humanitarian organizations led by volunteers and guided by its Congressional Charter and the Fundamental Principles of the International Red Cross Movement, to provide relief to victims of disaster and help people prevent, prepare for, and respond to emergencies.Anaphylaxis: An acute hypersensitivity reaction due to exposure to a previously encountered ANTIGEN. The reaction may include rapidly progressing URTICARIA, respiratory distress, vascular collapse, systemic SHOCK, and death.Relief Work: Assistance, such as money, food, or shelter, given to the needy, aged, or victims of disaster. It is usually granted on a temporary basis. (From The American Heritage Dictionary, 2d college ed)First Aid: Emergency care or treatment given to a person who suddenly becomes ill or injured before full medical services become available.Food Hypersensitivity: Gastrointestinal disturbances, skin eruptions, or shock due to allergic reactions to allergens in food.Blood DonorsDisaster Planning: Procedures outlined for the care of casualties and the maintenance of services in disasters.Mass Media: Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.Newspapers: Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)Negativism: State of mind or behavior characterized by extreme skepticism and persistent opposition or resistance to outside suggestions or advice. (APA, Thesaurus of Psychological Index Terms, 1994)Television: The transmission and reproduction of transient images of fixed or moving objects. An electronic system of transmitting such images together with sound over a wire or through space by apparatus that converts light and sound into electrical waves and reconverts them into visible light rays and audible sound. (From Webster, 3rd ed)Journalism: The collection, preparation, and distribution of news and related commentary and feature materials through such media as pamphlets, newsletters, newspapers, magazines, radio, motion pictures, television, and books. While originally applied to the reportage of current events in printed form, specifically newspapers, with the advent of radio and television the use of the term has broadened to include all printed and electronic communication dealing with current affairs.NewsEmotions: Those affective states which can be experienced and have arousing and motivational properties.Politics: Activities concerned with governmental policies, functions, etc.New YorkFacies: The appearance of the face that is often characteristic of a disease or pathological condition, as the elfin facies of WILLIAMS SYNDROME or the mongoloid facies of DOWN SYNDROME. (Random House Unabridged Dictionary, 2d ed)Crosses, Genetic: Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.New York CityDental Waste: Any waste product generated by a dental office, surgery, clinic, or laboratory including amalgams, saliva, and rinse water.La Crosse virus: A serotype of the species California encephalitis virus (ENCEPHALITIS VIRUS, CALIFORNIA), in the genus ORTHOBUNYAVIRUS, causing human MENINGOENCEPHALITIS. This is the agent most responsible for California encephalitis (ENCEPHALITIS, CALIFORNIA), the most prevalent mosquito-borne disease recognized in the United States.Palladium: A chemical element having an atomic weight of 106.4, atomic number of 46, and the symbol Pd. It is a white, ductile metal resembling platinum, and following it in abundance and importance of applications. It is used in dentistry in the form of gold, silver, and copper alloys.Borates: Inorganic or organic salts and esters of boric acid.Fluorine Compounds: Inorganic compounds that contain fluorine as an integral part of the molecule.Hydrocarbons, IodinatedPhosphines: Inorganic or organic compounds derived from phosphine (PH3) by the replacement of H atoms. (From Grant & Hackh's Chemical Dictionary, 5th ed)Catalysis: The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.Molecular Structure: The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.Rubber: A high-molecular-weight polymeric elastomer derived from the milk juice (LATEX) of HEVEA brasiliensis and other trees and plants. It is a substance that can be stretched at room temperature to at least twice its original length and after releasing the stress, retract rapidly, and recover its original dimensions fully.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Chaperonin 10: A group I chaperonin protein that forms a lid-like structure which encloses the non-polar cavity of the chaperonin complex. The protein was originally studied in BACTERIA where it is commonly referred to as GroES protein.Pasteurellosis, Pneumonic: Bovine respiratory disease found in animals that have been shipped or exposed to CATTLE recently transported. The major agent responsible for the disease is MANNHEIMIA HAEMOLYTICA and less commonly, PASTEURELLA MULTOCIDA or HAEMOPHILUS SOMNUS. All three agents are normal inhabitants of the bovine nasal pharyngeal mucosa but not the LUNG. They are considered opportunistic pathogens following STRESS, PHYSIOLOGICAL and/or a viral infection. The resulting bacterial fibrinous BRONCHOPNEUMONIA is often fatal.Automobiles: A usually four-wheeled automotive vehicle designed for passenger transportation and commonly propelled by an internal-combustion engine using a volatile fuel. (Webster, 1973)Ships: Large vessels propelled by power or sail used for transportation on rivers, seas, oceans, or other navigable waters. Boats are smaller vessels propelled by oars, paddles, sail, or power; they may or may not have a deck.Autoantibodies: Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.Immunoglobulin Idiotypes: Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.Hybridomas: Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.Lupus Erythematosus, Systemic: A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.Antibodies, Anti-Idiotypic: Antibodies which react with the individual structural determinants (idiotopes) on the variable region of other antibodies.Antibodies, Antinuclear: Autoantibodies directed against various nuclear antigens including DNA, RNA, histones, acidic nuclear proteins, or complexes of these molecular elements. Antinuclear antibodies are found in systemic autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, scleroderma, polymyositis, and mixed connective tissue disease.Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Hawks: Common name for many members of the FALCONIFORMES order, family Accipitridae, generally smaller than EAGLES, and containing short, rounded wings and a long tail.ChicagoBulimia: Eating an excess amount of food in a short period of time, as seen in the disorder of BULIMIA NERVOSA. It is caused by an abnormal craving for food, or insatiable hunger also known as "ox hunger".Raptors: BIRDS that hunt and kill other animals, especially higher vertebrates, for food. They include the FALCONIFORMES order, or diurnal birds of prey, comprised of EAGLES, falcons, HAWKS, and others, as well as the STRIGIFORMES order, or nocturnal birds of prey, which includes OWLS.BostonEagles: Large members of the FALCONIFORMES order of birds, family Accipitridae, most especially the genera Aquila, Haliaeetus, Harpia, and Circaetus. They are characterized by their powerful talons, which carry long, curved, pointed claws and by their opposable hindtoe.Falconiformes: An order of diurnal BIRDS of prey, including EAGLES; HAWKS; buzzards; vultures; and falcons.

Highly sensitive quantitation of methamphetamine by time-resolved fluoroimmunoassay using a new europium chelate as a label. (1/8142)

A simple and highly sensitive time-resolved fluoroimmunoassay of methamphetamine (MA) using a new fluorescent europium chelate (BHHCT-Eu3+) as a label is described. Two variations of competitive immunoassay were attempted. In the first (one-step) assay, microtiter plates coated with anti-MA were used, and the new label was bound to a conjugate of bovine serum albumin and N-(4-aminobutyl)-MA (MA-BSA). In the second (two-step) assay, instead of the labeled MA-BSA, biotinylated MA-BSA and BHHCT-Eu3+-labeled streptavidin-BSA were used. The lowest measurable concentrations of MA for the one-step and the two-step methods were 1 ng/mL (25 pg/assay) and 1 pg/mL (25 fg/assay), respectively. These were 10 to 1000 times superior to the detection limits of MA in any other immunoassay. Intra-assay coefficient of variation was approximately 2-8% at eight different concentrations (n = 4). Analysis of 34 urine samples with the new method and conventional gas chromatography showed a good correlation (r = 0.954). The high detectability of the present assay also enabled segmental hair analysis with a few centimeters of a hair.  (+info)

The levels and bactericidal capacity of antibodies directed against the UspA1 and UspA2 outer membrane proteins of Moraxella (Branhamella) catarrhalis in adults and children. (2/8142)

The UspA1 and UspA2 proteins from Moraxella catarrhalis share antigenic epitopes and are promising vaccine candidates. In this study, the levels and bactericidal activities of antibodies in sera from healthy adults and children toward UspA1 and UspA2 from the O35E strain were measured. Human sera contained antibodies to both proteins, and the levels of immunoglobulin G (IgG) antibodies were age dependent. Adult sera had significantly higher titers of IgG than child sera (P < 0.01). The IgG3 titers to the UspA proteins were higher than the IgG1 titers in the adults' sera, while the IgG1 titers were higher than the IgG3 titers in the children's sera (P < 0.05). The IgG antibodies in the sera from 2-month-old children appeared to be maternally derived, since the mean titer was significantly higher than that in sera from 6- to 7-month-old children (P < 0.05). Serum IgA antibodies to both UspA1 and UspA2 were low during the first 7 months of age but thereafter gradually increased along with the IgG titers. Analysis of sera absorbed with UspA1 or UspA2 showed that the antibodies to UspA1 and UspA2 were cross-reactive with each other and associated with serum bactericidal activity. Examination of affinity-purified human antibodies confirmed that naturally acquired antibodies to UspA1 and UspA2 were bactericidal and cross-reactive. These results support using UspA1 and UspA2 in a vaccine to prevent M. catarrhalis infections.  (+info)

Autoantibodies to RNA polymerases recognize multiple subunits and demonstrate cross-reactivity with RNA polymerase complexes. (3/8142)

OBJECTIVE: To determine the subunit specificity of autoantibody directed to RNA polymerases (RNAP) I, II, and III, which is one of the major autoantibody responses in patients with systemic sclerosis (SSc). METHODS: Thirty-two SSc sera with anti-RNAP antibodies (23 with anti-RNAP I/III, 5 with anti-RNAP I/III and II, and 4 with anti-RNAP II alone) were analyzed by immunoblotting using affinity-purified RNAP and by immunoprecipitation using 35S-labeled cell extracts in which RNAP complexes were dissociated. Antibodies bound to individual RNAP subunits were eluted from preparative immunoblots and were further analyzed by immunoblotting and immunoprecipitation. RESULTS: At least 15 different proteins were bound by antibodies in anti-RNAP-positive SSc sera in various combinations. All 9 sera immunoprecipitating RNAP II and all 28 sera immunoprecipitating RNAP I/III recognized the large subunit proteins of RNAP II and III, respectively. Reactivity to RNAP I large subunits was strongly associated with bright nucleolar staining by indirect immunofluorescence. Affinity-purified antibodies that recognized a 62-kd subunit protein cross-reacted with a 43-kd subunit protein and immunoprecipitated both RNAP I and RNAP III. Antibodies that recognized a 21-kd subunit protein obtained from sera that were positive for anti-RNAP I/III and II antibodies immunoprecipitated both RNAP II and RNAP III. CONCLUSION: Anti-RNAP antibodies recognize multiple subunits of RNAP I, II, and III. Moreover, the results of this study provide the first direct evidence that antibodies that recognize shared subunits of human RNAPs or epitopes present on different human RNAP subunits are responsible for the recognition of multiple RNAPs by SSc sera.  (+info)

Human geranylgeranyl diphosphate synthase. cDNA cloning and expression. (4/8142)

Geranylgeranyl diphosphate (GGPP) synthase (GGPPSase) catalyzes the synthesis of GGPP, which is an important molecule responsible for the C20-prenylated protein biosynthesis and for the regulation of a nuclear hormone receptor (LXR.RXR). The human GGPPSase cDNA encodes a protein of 300 amino acids which shows 16% sequence identity with the known human farnesyl diphosphate (FPP) synthase (FPPSase). The GGPPSase expressed in Escherichia coli catalyzes the GGPP formation (240 nmol/min/mg) from FPP and isopentenyl diphosphate. The human GGPPSase behaves as an oligomeric molecule with 280 kDa on a gel filtration column and cross-reacts with an antibody directed against bovine brain GGPPSase, which differs immunochemically from bovine brain FPPSase. Northern blot analysis indicates the presence of two forms of the mRNA.  (+info)

Serum dilution neutralization test for California group virus identification and serology. (5/8142)

The serum dilution neutralization test was evaluated for serological diagnosis of California group arbovirus infections and identification of virus isolates. The technical advantages and the degree of subtype specificity of the serum dilution neutralization test over the hemagglutination inhibition test and the complement fixation test were demonstrated with paired specimens from human cases, single human survey sera, and sentinel rabbit sera. Twenty-one virus isolates from various geographical areas of the United States were also used to evaluate the efficacy of the serum dilution neutralization test for specific virus identification.  (+info)

Induction of lasting complete regression of preformed distinct solid tumors by targeting the tumor vasculature using two new anti-endoglin monoclonal antibodies. (6/8142)

Endoglin (EDG, CD105) is a proliferation-associated antigen on endothelial cells. In this study, two new anti-EDG monoclonal antibodies (mAbs) Y4-2F1 (or termed SN6j) and P3-2G8 (SN6k) were generated and used for treating distinct preformed tumors. These mAbs, both IgG1-kappa antibodies, cross-reacted weakly with mouse endothelial cells but defined epitopes different from the epitope defined by a previously reported anti-EDG mAb K4-2C10 (B. K. Seon et al., Clin. Cancer Res., 3: 1031-1044, 1997). SN6j and SN6k reacted strongly with human endothelial cells and vascular endothelium of malignant human tissues but showed no significant reactivity with tumor cells per se. The deglycosylated ricin A chain (dgRA) conjugates of the two mAbs showed a weak but specific cytotoxic activity against murine endothelial cells in vitro. In the therapeutic studies, severe combined immunodeficient mice were inoculated s.c. with MCF-7 human breast cancer cells and left untreated until palpable tumors of distinct size (4-6 mm in diameter) appeared. Mice with the distinct tumors were treated by i.v. administration of individual anti-EDG conjugates, unconjugated mAbs, or a control conjugate. Long-lasting complete regression of the tumors was induced in the majority of tumor-bearing mice (n = 8 for each conjugate) when 40 microg of the individual conjugates were administered three times via the tail vein. It is remarkable that the tumors remained regressed without further therapy for as long as the mice were followed (i.e., 100 days). Control conjugate did not induce regression of the tumors in any of the treated mice, although weak nonspecific effects were observed in some of the mice (n = 8). The effects of unconjugated mAbs were small with the dose used, i.e., 34 microg three times. The anti-EDG conjugates showed antiangiogenic activity in the dorsal air sac assay in mice. The results suggest good potential of these conjugates for the clinical application.  (+info)

A new picornavirus isolated from bank voles (Clethrionomys glareolus). (7/8142)

A previously unknown picornavirus was isolated from bank voles (Clethrionomys glareolus). Electron microscopy images and sequence data of the prototype isolate, named Ljungan virus, showed that it is a picornavirus. The amino acid sequences of predicted Ljungan virus capsid proteins VP2 and VP3 were closely related to the human pathogen echovirus 22 (approximately 70% similarity). A partial 5' noncoding region sequence of Ljungan virus showed the highest degree of relatedness to cardioviruses. Two additional isolates were serologically and molecularly related to the prototype.  (+info)

Serological classification of Pseudomonas aeruginosa by a slide agglutination test. (8/8142)

Serological classification of Pseudomonas aeruginosa by the slide agglutination test with live organisms was studied, based on the O antigen schema adopted by the international expert panel sponsored by the Subcommittee on Pseudomonas and Related Organisms of the International Committee on Systematic Bacteriology. The typing results obtained by the slide test with well-absorbed O sera were identical to those obtained by the conventional tube agglutination test with autoclaved organisms. Most O antigens occur singly; but O2, O5, and O16 occur in four combinations. Antigens O13 and O14 are closely related, as are O7 and O8, and it would be convenient to classify organisms possessing these antigens collectively as O7,8 and O13,14.  (+info)

Jeggo, M H. and Wardley, R C., "Generation of cross-reactive cytotoxic t lymphocytes following immunization of mice with various bluetongue virus types." (1982). Subject Strain Bibliography 1982. 3115 ...
In an assay for a target nucleotide sequence employing hybridization with a target probe nucleotide sequence, cross reaction between the target probe and a non-target nucleotide sequence, capable of hybridizing with the target probe, is prevented or diminished by introducing, at some point during the hybridization, a non-target probe which hybridizes preferentially with the non-target nucleotide sequence. The melting temperature for the non-target probe/non-target nucleotide sequence hybrid is preferably greater than that of the target probe/non-target sequence hybrid. The hybridization can be carried out under isothermal conditions or preferentially with gradual cooling.
TY - JOUR. T1 - Immunological cross reaction between calf and Drosophila histones. AU - Bustin, M.. AU - Reeder, R. H.. AU - McKnight, S. L.. PY - 1977. Y1 - 1977. UR - http://www.scopus.com/inward/record.url?scp=0017387756&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0017387756&partnerID=8YFLogxK. M3 - Article. C2 - 856814. AN - SCOPUS:0017387756. VL - 252. SP - 3099. EP - 3101. JO - Journal of Biological Chemistry. JF - Journal of Biological Chemistry. SN - 0021-9258. IS - 9. ER - ...
Heterosubtypic immunity, defined as cross-reactive immune responses to influenza virus of a different serotype than the virus initially encountered, was investigated in association with virus-specific cytotoxic T lymphocyte (CTL) responses induced in systemic and mucosa-associated lymph nodes after immunization via different routes. Mice immunized by the pulmonary route with live nonpathogenic influenza virus, strain Udorn (H3N2), survived challenge with mouse-adapted pathogenic influenza virus, strain PR/8/34 (H1N1). These mice developed strong heterosubtypic CTL responses in spleen, cervical lymph nodes (CLN), and mediastinal lymph nodes (MLN). Alternately, only 20% of mice immunized intravenously, intraperitoneally, or intranasally survived the challenge; all of these developed CTL responses in spleen and CLN, but not in MLN. Direct correlation between short-term and long-term memory heterosubtypic CTL responses induced in MLN and host recovery after lethal infection indicates that these CTL
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
To assess herd immunity to swine influenza viruses, we determined antibodies in 28 paired serum samples from participants in a prospective serologic cohort study in Hong Kong who had seroconverted to pandemic (H1N1) 2009 virus. Results indicated that infection with pandemic (H1N1) 2009 broadens cross-reactive immunity to other recent subtype H1 swine viruses ...
Effect Diluent™ for dilution of bio samples, to avoid Hook Effects, reduce matrix effects and ensure that the signal response is within the standard curve.
1. Antisera to R-salt-azo-benzidine-azo-crystalline egg albumin give precipitates with crystalline egg albumin by virtue of their antidye content.. 2. The quantitative course of the reactions with increasing amounts of antigen is very similar for the dye-antidye and egg albumin-anti-egg albumin systems, but differs markedly for the cross reaction between egg albumin and antidye.. 3. A possible explanation for the occurrence of this one-sided crossreaction is given in terms of reactive groupings on the antigen and antibody.. 4. A qualitative expression of the course of the cross-reaction is given in terms of the laws of classical chemistry.. ...
Heterosubtypic T cell-mediated immunity to influenza A viruses in humans has been widely reported (24-26, 36). While cross-reactive T cells cannot prevent establishment of infection, they can enhance recovery by promoting virus clearance and reduce the severity of illness (15). It has also been demonstrated that in vitro T cell cultures derived from healthy individuals can lyse target cells infected with swine or avian influenza viruses (28, 29). Together with a large number of studies in mice that demonstrated cross-protection conferred by influenza A-specific T cells during subsequent challenges (reviewed in ref. 11), these studies suggest that vaccines stimulating cross-reactive T cell populations, potentially used in conjunction with the current antibody-based strategy, may confer broader and more effective protection against a potential pandemic strain. However, most studies using T cell cultures expanded in vitro were unable to assess accurately the overall influenza-specific memory T cell ...
Control of swine influenza by vaccination has become more difficult in recent decades, as the evolution of the virus has resulted in inconsistent responses to traditional vaccines. Standard commercial swine flu vaccines are effective in controlling the infection when the virus strains match enough to have significant cross-protection, and custom (autogenous) vaccines made from the specific viruses isolated are created and used in the more difficult cases.[80][81] Present vaccination strategies for SIV control and prevention in swine farms typically include the use of one of several bivalent SIV vaccines commercially available in the United States. Of the 97 recent H3N2 isolates examined, only 41 isolates had strong serologic cross-reactions with antiserum to three commercial SIV vaccines. Since the protective ability of influenza vaccines depends primarily on the closeness of the match between the vaccine virus and the epidemic virus, the presence of nonreactive H3N2 SIV variants suggests that ...
Self-reactive CD4 T cells are thought to have a central role in the pathogenesis of many chronic inflammatory human diseases. Microbial peptides can activate self-reactive T cells, but the structural basis for such crossreactivity is not well understood. The Hy.1B11 T cell receptor (TCR) originates from a patient with multiple sclerosis and recognizes the self-antigen myelin basic protein. Here we report the structural mechanism of TCR crossreactivity with two distinct peptides from human pathogens. The structures show that a single TCR residue (CDR3α F95) makes the majority of contacts with the self-peptide and both microbial peptides (66.7-80.6%) due to a highly tilted TCR-binding topology on the peptide-MHC surface. Further, a neighbouring residue located on the same TCR loop (CDR3α E98) forms an energetically critical interaction with the MHC molecule. These data show how binding by a self-reactive TCR favors crossreactivity between self and microbial antigens.
Full FDA Tissue Panel of high quality normal tissues for antibody cross-reactivity studies. Multiple organs from a range of age and gender-specific donors.
Transglutaminase 2 (TG2) is a ubiquitously expressed enzyme that catalyzes the posttranslational modification of glutamine residues on protein or peptide substrates. 3-bromo-4 5 (DHI) scaffold have been widely used to study TG2 biology and are well tolerated in vivo but these compounds have only modest potency and their selectivity toward other transglutaminase homologues is largely unknown. In the present work we first profiled the selectivity of existing inhibitors against the most pertinent TG isoforms (TG1 TG3 and FXIIIa). Significant cross-reactivity of these small molecules with TG1 was observed. Structure-activity and ?selectivity analyses led to the identification of modifications that improved potency and isoform selectivity. Preliminary pharmacokinetic analysis of the most promising analogues was also undertaken. Our new data provides a clear basis for the rational selection of dihydroisoxazole inhibitors as tools for in vivo biological investigation. Kitl Introduction The mammalian ...
TY - JOUR. T1 - Epidemics in Competition. T2 - Partial Cross-Immunity. AU - Andreasen, Viggo. PY - 2018. Y1 - 2018. N2 - The competition between two pathogen strains during the course of an epidemic represents a fundamental step in the early evolution of emerging diseases as well as in the antigenic drift process of influenza. The outcome of the competition, however, depends not only on the epidemic properties of the two strains but also on the timing and size of the introduction, characteristics that are poorly captured by deterministic mean-field epidemic models. We describe those aspects of the competition that can be determined from the mean-field models giving the range of possible final sizes of susceptible hosts and cumulated attack rates that could be observed after an epidemic with two cross-reacting strains. In the limit where the size of the initial infection goes to zero, the possible outcomes lie on a (one dimensional) curve in the outcome space.. AB - The competition between two ...
Interleukin-5 (IL-5) is a hematopoietic growth factor expressed in Th2, mast cells and eosinophils. IL-5 acts through its receptor, the IL-5 receptor (IL-5R) and is involved in B-cell growth and eosinophil activation. IL-5 has been shown to be and is regulated by GATA-3, in addition to other transcription factors. Human and mouse IL-5 are cross-reactive. Recombinant mouse IL-5 is a non-glycosylated, disulfide-linked homodimer. It is comprised of two 113 amino acid chains with a total molecular weight of 26.2 kDa. Ships ambient. Store frozen at -20 to -10C. ...
A gray area at times. Have used the Scytek kit (check out website) for frozen sections, but did not use their peroxidase blocker, it ate frozens off the slide. Substituted a Dako peroxidase blocker for frozens was much friendlier. The kicker with this kit, their special blocker is excellent, and you have to use their chromogen with this kit. What really worked for us was to use their two blockers (not the peroxidase block though),a biotinylated primary in place of a secondary antibody with the Scytek kit since we did not have a purified primary on hand. We also diluted out the biotinylated primary further, so do a more extended dilution panel, this reduced our cross reaction background. This is the basis of the DAKO ARK kit, biotinylating the primary, some antibodies don biotinylate as well, a potential problem, but may work with yours, so another option - DAKO ARK kit. There are others on the market, Zymed, Innovex, Vector MOM kit, etc, all worth a try. Have heard some mixed reviews on Mouse ...
Species: Mouse Monoclonal Clone: 1A4 Isotype: IgG2a,k Species Reactivity: Human, Baboon, Cow, Rabbit, Mouse, Rat, Chicken. Positive Control: Blood vessels Specificity: This antibody recognizes the a-smooth muscle form of actin. It shows no cross reaction with actin from fibroblasts (b- and g-cytoplasmic), striated muscle (a-sarcometric), and myocardium (a-myocardial). Its epitope is composed of the acetyl group and the first 4 amino acids on the N-terminal end of the peptidic chain of a-smooth actin. ScyTek s 1A4 stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained as they also contain this actin ...
Species: Mouse Monoclonal Clone: 1A4 Isotype: IgG2a,k Species Reactivity: Human, Baboon, Cow, Rabbit, Mouse, Rat, Chicken. Positive Control: Blood vessels Specificity: This antibody recognizes the a-smooth muscle form of actin. It shows no cross reaction with actin from fibroblasts (b- and g-cytoplasmic), striated muscle (a-sarcometric), and myocardium (a-myocardial). Its epitope is composed of the acetyl group and the first 4 amino acids on the N-terminal end of the peptidic chain of a-smooth actin. ScyTek s 1A4 stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained as they also contain this actin ...
Although Ag-Ab reactions are highly specific, in some cases antibody elicited by one antigen can cross-react with an unrelated antigen. Such cross-reactivity
Hello, I work with fish in a resarch lab and with some of the antibodies I use I get what I would consider non-specific crossreactivity with mucus either covering the body surface or in mucocyte granules. More often this problem would arise with rabbit polyclonal anti-peptide antibodies using neat serum. I usually get around this by affinity purifying using the peptide but in some cases this is not possible. Someone once told me that mucoproteins have a large number of antigenic determinants increasing the likelyhood of crossreactivity but I could never find a reference. Ive searched through the archives but couldnt find an explanation except for a suggestion that it might be charge related. Could someone please shed some light on this situation for me. It is something that has bugged me for the longest time. Thank you for any insight. Sincerely, Jon _______________________________________________ Histonet mailing list [email protected] ...
Publications 164 Publications PUBLICATIONS 1. Heemskerk B, Veltrop-Duits LA, van Vreeswijk T, ten Dam MM, Heidt S, Toes RE, van Tol MJ, Schilham MW. Extensive cross-reactivity of CD4 + adenovirus-specific
Biomarker studies which were previously limited by poor assay sensitivity, low throughput systems and insufficient sample amount are now feasible in a simple and efficient way.. For the first time it is possible to do high capacity quantification of 92 protein biomarkers in 96 samples. Conventional immunoassays have been unable to scale due to increasing antibody cross-reactivity but TATAA Biocenter offers a solution using the Multiplex platform from Olink Proteomics. It generates high quality data even in highly multiplexed formats and the opportunities are enormous for powerful analysis of multivariate biomarker patterns.. ...
Supplier: Assaypro • Crossreactivity: Bo, Ms, Rt, Pg, Mk, Dg, Rb • Format: Sandwich ELISA • Range: 3.125-200 ng/mL • Min. Sample Size: 50 uL • Sample Types: U, M, Sv, ...
In this thesis the role of bacteria for the induction of autoimmunity was investigated. In detail, it was examined whether bacteria are able to activate autoreactive CD4+-T-cells antigen-specific ("cross-reactivity") or antigen-unspecific ("bystander-activation"). It was shown that the examined transgenic MBP-peptide specific T-cell-receptor recognized many natural occurring cross-reactive peptides of microbial origin, which induced an activation of the T-cells in vitro and which could induce autoimmune encephalomyelitis (EAE) in the T-cell-receptor transgenic mice in vivo. Furthermore, it was examined, whether lipopolysaccharide (LPS) as activator of the innate immune system could induce an unspecific activation of the autoreactive T-cells in vitro and whether administration of LPS in the transgenic mice could induce EAE in vivo. It was shown that LPS activates a small percentage of CD4+ - T-cells. Application of LPS to the transgenic T+alpha- mice induced EAE. Therefore, the role of ...
Despite inheriting half of its genetic material from its father, the fetoplacental unit is not rejected by the maternal immune system because of a unique immunological relationship with the mother
The properties of high affinity and specificity of antibodies are successfully employed in assays used for quantification of specific analytes in complex mixtures. The method is indirect in the sense that it is the antibody-analyte complex that is detected rather than the analyte per se. However, if the quality of the antibody is good, i.e., low cross reactivity with other molecules, there is a strong relationship between the measured signal from the complex and the concentration of the analyte. This relationship can be determined by the use of calibrators with known concentrations, which allow for a standard curve to be constructed and the concentration in a sample to be calculated.. ...
anti-DJ-1 (PARK7), mAb (1D7) is a monoclonal antibody that crossreacts with human, mouse, rat protein. Works in ELISA, WB. Important for Inflammation, Oxidative Stress, ROS, Immunology research.
Citoxlab provides GLP-compliant Tissue Cross-Reactivity (TCR) studies for biologicals and monoclonals with all the tissues recommended by the FDA and EMEA.
anti-BEDF, mAb (PK33-5B8) is a monoclonal antibody that crossreacts with human protein. Works in WB, IP. Important for Inflammation, Oxidative Stress, ROS, Immunology research.
, Rat Serum - 50mL, GTX73216, Applications: Blocking, ELISA, ICC/IF, IHC, WB; Blocking, ELISA, Immunocytochemistry/ Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Western Blot (WB); CrossReactivity:
, Human Brain: Amygdala (Normal) tissue lysate, GTX28766, Applications: ELISA, IP, WB; ELISA, Immunoprecipitation, Western Blot (WB); CrossReactivity:
Newcombe, J, Eales-Reynolds, L, Wootton, L, Gorringe, AR, Funnell, SGP, Taylor, SC and McFadden, JJ (2004) Infection with an avirulent phoP mutant of Neisseria meningitidis confers broad cross-reactive immunity ...
Three anti-horse liver alcohol dehydrogenase (HLADH) monoclonal antibodies are described. Two are specific for ADH and cross-react with class I and II enzymes from mouse, horse and Chinese hamster....
TY - JOUR. T1 - Formalin-inactivated EV71 vaccine candidate induced cross-neutralizing antibody against subgenotypes B1, B4, B5 and C4A in adult volunteers. AU - Chou, Ai Hsiang. AU - Liu, Chia Chyi. AU - Chang, Jui Yuan. AU - Jiang, Renee. AU - Hsieh, Yi Chin. AU - Tsao, Amanda. AU - Wu, Chien Long. AU - Huang, Ju Lan. AU - Fung, Chang Phone. AU - Hsieh, Szu Min. AU - Wang, Ya Fang. AU - Wang, Jen Ren. AU - Hu, Mei Hua. AU - Chiang, Jen Ron. AU - Su, Ih Jen. AU - Chong, Pele Choi Sing. PY - 2013/11/21. Y1 - 2013/11/21. N2 - Background: Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia. No effective EV71 vaccine is available. A randomized and open-label phase I clinical study registered with ClinicalTrials.gov #NCT01268787, aims to evaluate the safety, reactogenicity and immunogenicity of a formalin-inactivated EV71 vaccine candidate (EV71vac) at 5- and 10-μg doses. In this study we report the cross-neutralizing antibody responses from each ...
In a subset of patients with limited cutaneous (lc) systemic sclerosis (SSc), anti-CENP-A antibodies (Ab) cross-react with a peptide (FOXE3p53-62) that presents striking homology with one of the two immunodominant epitopes of CENP-A (Ap17-30). We searched for clinical correlates of anti-FOXE3p53-62 Ab by measuring their levels along with those of Ab to Ap17-30 and to the second immunodominant epitope of CENP-A, namely Ap1-17. Serum samples were obtained from 121 patients with SSc, 46 patients with systemic lupus erythematosus (SLE) and 25 healthy blood donors (HBD). The reactivity of serum IgG to Ap1-17, Ap17-30 and FOXE3p53-62 was measured by ELISA. The corresponding anti-peptide Ab were affinity-purified from pooled SSc sera and used to establish standard curves for quantifying these Ab in patients and HBD. Receiver operating characteristics (ROC) analysis, comparing SSc patients who were positive for anti-CENP Ab (ACA+) to those who were negative, was used to find cut-off points for dichotomizing the
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Abbkine Scientific has announced the launch of its new kit, the EliKine™ Human IL-2 ELISA Kit otherwise known as Human IL2 ELISA Kit. The launch of the product reiterates the companys commitment to enhancing research and investigation in the field of life science.. The product has a human reactivity, employing a two-site sandwich ELISA to quantitate IL-2 in samples. With colorimetric detection method and assay duration with multiple steps standard sandwich ELISA assay with a working time of 3-5 hours, the kit stands tall amongst its peers on the market.. The featured kit includes Human IL-2 microplate, Human IL-2 standard, Human IL-2 detect antibody, EliKine™ Streptavidin-HRP, Standard diluent, Assay buffer, HRP substrate, Stop solution, Wash buffer and Plate covers.. The major feature and benefit of the kit besides having a calibration range of 3.9 pg/ml-250 pg/ml is its high sensitivity and excellent specificity for detection of Human IL-2. It also has no significant cross-reactivity or ...
Human TNF-α ELISA Kit is the latest product from Abbkine Scientific Research Company. As part of the companys plans to ease the research process and ensure that better results are gotten in record time, the company made the announcement to official launch the Human TNF alpha ELISA Kit on the market.. The ELISA Kit is particularly designed to allow for the easy detection of Human TNF-α. Consequently, investigators, researchers and other such users of the product can easily get their desired results in record time. This also ensures that better results are gotten.. EliKine™ Human TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples, it has high sensitivity and excellent specificity for detection of Human TNF-α. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.. The recent launch of the Human TNF-α ELISA Kit has been described by many as a revolutionary introduction to the industry. With a high sensitivity and ...
Objectives. This study tested the hypothesis, first proposed by Chaussinand, that individual-level immunity acquired from exposure to tuberculosis may have contributed to the disappearance of leprosy from western Europe. Methods. The epidemiological consequences of cross-immunity were assessed by the formulation of a mathematical model of the...
Swine flu has been in the headlines for days now. The headlines change every day. By the time this article is printed, the situation will have changed even more. Therefore, it is hard to write a timely article on this particular disease. There are some facts that bear no relation to time. The first is that we have flu epidemics every year. They usually occur in the December to February time frame. We start an immunization program for it prior to that. The viruses contained in the immunization are scientists best guesses as to which flu viruses are going to be prevalent that particular year. They usually guess correctly so many people are protected before each years outbreak. One of the issues with swine flu is that there is currently no immunization for it. Cross reaction to other flu strains is not present. Therefore, no one is immune to the virus. For that reason many more people would be affected by an outbreak. The second fact is that every year people who get the flu die from its ...
Centralized" (ancestral and consensus) HIV-1 envelope immunogens induce broadly cross-reactive T cell responses in laboratory animals; however, their potential to elicit cross-reactive neutralizing antibodies has not been fully explored. Here, we report the construction of a panel of consensus subtype B (ConB) envelopes and compare their biologic, antigenic, and immunogenic properties to those of two wild-type Env controls from individuals with early and acute HIV-1 infection. Glycoprotein expressed from full-length (gp160), uncleaved (gp160-UNC), truncated (gp145), and N-linked glycosylation site deleted (gp160-201N/S) versions of the ConB env gene were packaged into virions and, except for the fusion defective gp160-UNC, mediated infection via the CCR5 co-receptor. Pseudovirions containing ConB Envs were sensitive to neutralization by patient plasma and monoclonal antibodies, indicating the preservation of neutralizing epitopes found in contemporary subtype B viruses. When used as DNA vaccines ...
An agglutination test used in the laboratory to diagnose rickettsial diseases. It depends on a nonspecific cross reaction between antibodies produced by the rickettsial infection with the OX-2, OX-19 and OXK antigens of the Gram negative rod, Proteus.. ...
The participants, recruited by advertisements in two daily newspapers, were obese and asthmatic. Inclusion criteria were ability to cope with the study protocol, body mass index 30-42, age 18-60 years, previously diagnosed asthma with a spontaneous diurnal variation or a bronchodilator response of 15% or more, and being a non-smoker or having stopped smoking for two years or more before age 50. Exclusion criteria were pregnancy, history of bulimia or anorexia, unstable angina or arrhythmia, untreated thyroid disease, symptomatic liver or gall bladder disorder, any other severe disease, insulin treatment, systemic steroid treatment, or history of food allergy or of intolerance to any component of the very low energy dietary preparation that would be used in the study-such as soya, fish, chocolate, or lactose. Participants with a history of adverse reactions to peas, beans, or peanuts were excluded because of possible cross reactions to soya protein.3. Telephone interviews were held with 202 ...
Doctor answers on Symptoms, Diagnosis, Treatment, and More: Dr. Killian on positive skin tb test: There is no cross reaction between them. By the way, up to 5% of normal individuals may test positive to ANA test, usually at low titer. for topic: Positive Skin Tb Test
A family of broadly neutralizing antibodies from a chronically infected donor provides a schematic for designing vaccines and treatments that target multiple strains of the virus.. 0 Comments. ...
A family of broadly neutralizing antibodies from a chronically infected donor provides a schematic for designing vaccines and treatments that target multiple strains of the virus.. 0 Comments. ...
Hi, I have a mouse anti-bovine glutamate dehydrogenase mAb and Im curious whether anyone would know whether it will crossreact with Human GDH. I think the two proteins are fairly similiar but how similiar are humans with cows??? Please send a reply to my E-mail dsho at med.unc.edu Thanx for any thoughts. Moo Moo, Dave Shock ...
Homo sapiens membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) (MCP), transcript variant h, mRNA. (H00004179-R27) - Products - Abnova
Homo sapiens membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) (MCP), transcript variant n, mRNA. (H00004179-R17) - Products - Abnova
We would like to thank Dr. Sylvain Lesne for his interest in our work and for his comments. We would like to clarify some of the points he raised concerning our report, since we disagree with some of his statements.. Regarding Aβ detection, we used the 6E10 antibody to monitor the level of APP transgene expression at the age we performed our analysis of spine density in vivo (i.e., three months postnatal), but our intention was not to characterize the presence of Aβ1-42 oligomers at this age in this mouse strain. They have been extensively characterized previously by others (see below). The 6E10 antibody is known to recognize human APP, CTF-β, and Aβ. We acknowledge that the band running below 15 kDa should be considered as a mixture of CTF/Aβ since tissues were homogenized in RIPA buffer. Regarding the APP signal at ~100 kDa, we could clearly detect a faint signal in the wild-type mice (after overexposure of the membrane), suggesting that the antibody can cross-react with mouse APP. This ...
Browse Item # 73-364, LRRTM2 in the Antibodies, Inc. catalog including Item #,Item Name,Description,Target,Applications,Clone,IgG Isotype,Species Reactivity,Validation,Type,Format,Cross Reactivity,Expected Banding Pattern,Host,Label,Antibody Type,Com
Browse Item # 73-136, GABA(A)R, Alpha1 in the Antibodies, Inc. catalog including Item #,Item Name,Description,Target,Applications,Clone,IgG Isotype,Species Reactivity,Validation,Type,Format,Cross Reactivity,Expected Banding Pattern,Host,Label,Antibod
Anne Schuchat: I think one possibility is that older people might have been exposed to H1N1 strains a long time ago that might have some relation to the strain were seeing now. Our virologists have compared it to all these other strains and this is really different. This is not a close, genetic relative of any of the H1N1s that have been a problem in people, but the immunologic characteristics might be different than the genetic characteristics and so it would be very great if older people had been exposed to some H1N1 a long time ago that had some relationship with this one immunologically and that might lead to that pre-existing immune they we hope is there, but were not sure is there. So we dont have a particular virus that were thinking about because the genetic characteristics are so different, but we are wondering about whether there were some viruses around back in the 30s, 40s or 50s that might be immunologically similar to the one were seeing now ...
Preamble: Stanford points out that the average 70-year-old is on 7 (seven) pharmaceutical agents1. Since literally hundreds of potential adverse reactions can occur with each medication, imagine the potential for cross reactivity (same reference quotes an average number of potential side effects per drug to be 69)…especially if a drugs metabolite can impart adverse reactions too?
1P7K: Structure of an anti-DNA fab complexed with a non-DNA ligand provides insights into cross-reactivity and molecular mimicry.
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Since August 2011, a total of 12 human infections with influenza A (H3N2) variant viruses with genes from avian, swine, and human viruses (i.e., A [H3N2]v) that had acquired the M gene from influenza A (H1N1)pdm09 virus have been reported to CDC. Eleven of the cases occurred in children aged ,10 years. In six cases, no history of recent exposure to swine was noted, suggesting that human-to-human transmission had occurred (1-3). This new gene constellation for A (H3N2)v viruses and its temporal association with an increase in human cases of A (H3N2)v highlight the need to better understand the risk for human infection with these viruses and the extent to which current seasonal vaccines might elicit cross-reactive antibodies to them. CDC conducted a preliminary analysis to evaluate the age-specific presence of serum cross-reactive antibody in U.S. populations vaccinated or not vaccinated with the 2010-11 seasonal trivalent influenza vaccine (TIV). The results indicated that 1) little or no ...
Control of swine influenza by vaccination has become more difficult in recent decades, as the evolution of the virus has resulted in inconsistent responses to traditional vaccines. Standard commercial swine flu vaccines are effective in controlling the infection when the virus strains match enough to have significant cross-protection, and custom (autogenous) vaccines made from the specific viruses isolated are created and used in the more difficult cases.[80][81] Present vaccination strategies for SIV control and prevention in swine farms typically include the use of one of several bivalent SIV vaccines commercially available in the United States. Of the 97 recent H3N2 isolates examined, only 41 isolates had strong serologic cross-reactions with antiserum to three commercial SIV vaccines. Since the protective ability of influenza vaccines depends primarily on the closeness of the match between the vaccine virus and the epidemic virus, the presence of nonreactive H3N2 SIV variants suggests that ...
Extensive cross-reactivity between the different individual species of the genus could be expected, but has not been formally evaluated. (8). A class I chitinase has been reported to be the relevant protein linked to cross-reactions in latex-fruit allergy syndrome, which includes passion fruit, cherimoya, kiwi, papaya, mango, tomato and wheat. (5, 9). A class I chitinase from avocado or latex extract was used as an inhibitor in a study the aim of which was to evaluate the role of chitinases and complex glycans as cross-reactive determinants linked to latex-food allergy. Putative class I chitinases of 30 to 45 kDa were recognised by both specific polyclonal antibodies to chitinases and sera from patients with latex-fruit allergy in chestnut, cherimoya, passion fruit, kiwi, papaya, mango, tomato, and wheat flour extracts. Prs a 1, the major allergen and class I chitinase from avocado, along with the latex extract, strongly or fully inhibited IgE binding by these components when tested in ...
Blair, P B.; Weiss, D W.; and Smith, G H., "Studies on antigenic cross-reactivity of oncogenic rna viruses. Cross-reactions between mouse mammary tumor viruses from different mouse strains." (1970). Subject Strain Bibliography 1970. 940 ...
We determined the antigenic specificity and protective immunogenicity of two chemically synthesized peptides of type 5 streptococcal M protein. The synthetic peptides, designated S-M5(1-20) and S-M5(20-40), represent the amino-terminal amino acid sequence of the native pepsin-extracted M5 molecule, which is known to contain at least one heart cross-reactive epitope. Initial studies showed that neither of the synthetic peptides was able to bind purified heart-reactive M5 antibodies. In addition, S-M5(1-20), but not S-M5(20-40), contained type-specific antigenic determinants as measured by enzyme-linked immunosorbent inhibition assays. When covalently linked to tetanus toxoid, S-M5(1-20), but not S-M5(20-40), evoked significant levels of type-specific, opsonic (and presumably protective) antibodies in rabbits without evoking heart cross-reactive antibodies. ...
Sandwich ELISA is a highly sensitive method that can be used to determine if two epitopes are part of the same macromolecule or supramolecular complex. In the case of plant cell wall glycans, it can reveal the existence of inter-polymers linkages, leading to better understanding of overall cell wall architectures. This development of a conventional sandwich ELISA protocol uses a carbohydrate-binding module (CBM), a small protein domain found in some carbohydrate catalysing or activating enzymes, and rat monoclonal antibodies (mAbs) which can be combined in the same ELISA plate without risk of cross reaction; the secondary anti-rat HRP antibody being only able to bind to the rat mAb and not the CBM. This protocol was developed and modified in the Prof. J. Paul Knox lab at the University of Leeds.
The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in BL21. EV71 contamination had been 90.1, 98.4, 98.8 and 88.7%, respectively; likewise, those of IgG antibodies in serum examples had been 82.4, 89.1, 91.5 and 78.1%, respectively. Five of 80 examples (6.25%) from CA16-infected sufferers were detected positive by ELISA with recombinant VP1 proteins where indicated the cross reactions and 0 of 5 examples from sufferers infected with other enteroviruses including CA4, CA5, CB3, CB5 and echovirus 6. As a result, the recombinant VP1 proteins of EV7l might provide a theoretical guide for building a highly effective antibody testing of IgM for EV71-contaminated patients with medically suspected hand, feet, and mouth area disease (HFMD). that may cause severe problems, from minor HFMD to serious neurological syndromes, such as for example encephalitis, pulmonary edema, and death even. Outbreaks of EV71 infections have already been reported ...
So often in veterinary medicine, as in other medical fields, we are looking for diagnostic tests to aid in treatment and to prognosticate for various diseases. Both infectious and non-infectious diseases may be diagnosed by detecting the causative agent, clinical signs, pathologic changes, biochemical changes and/or surrogate evidence of past or present exposure to an agent (antibody). A large number of the tests that we use are able to detect antibody, however, our dilemma is whether that is what is currently producing the clinical signs or is it a past infection. Alternatively, is the reaction we see a cross reaction to some other agent. Seldom do we think about the test in terms of what information it is going to provide basing our recommendations on the prevalence of the disease in your particular practice area. This is commonly referred to in epidemiology circles as the pre-test probability of the disease in question. Prevalence of disease is different for every area, and will differ in the ...
Whether tumors of different lineages share common Ags is a critical issue for understanding anti-tumor immune responses and for designing Ag-specific tumor immunotherapy. Because of lack of cross-protection among individually derived tumors, it has been proposed that tumor Ags are specific for individual tumors. Here we show that lack of cross-protection is not due to lack of a shared tumor Ag. Thus, a plasmocytoma J558 transfected with the costimulatory molecule B7 activates a cross-reactive CTL response in vivo. The major Ag recognized by the cross-reactive CTL is P1A, which is expressed in mastocytoma P815, plasmocytoma J558, and fibrosarcoma Meth A. Surprisingly, no significant cross-protection can be detected among P1A-expressing tumors after immunization with either P1A-expressing or B7-transfected P815 cells. Our results demonstrate that multiple lineages of tumors are not cross-protected even though they share a tumor Ag that can be recognized by CTL. These results have important ...
SGS, a leading bio/pharmaceutical, analytical and bioanalytical contract solutions provider, has announced that it has invested in multiple highly sensitive and selective LC-MS/MS instruments at its Poitiers, France, laboratory, for the quantification of proteins and peptide therapeutics.. The two systems, which are now installed and validated, are a combination of the AB SCIEX 6500+ and Shimadzu X2 UPLC, which offers high specificity, a wide dynamic range, faster method development and the ability to quantify multiple proteins simultaneously.. LC-MS/MS techniques have a number of advantages compared with standard conventions for protein and peptide quantitation, which are based on ligand-binding assays (LBAs) such as the enzyme-linked immunosorbent assay (ELISA).. Assay development can often be time-consuming and expensive and, in addition, LBA results are often plagued by interferences and high background from antibody cross-reactivity.. "Mass spectrometry platforms have universal ...
Cross-reactive cells are oligoclonal T memory cell expansions. (A) Cross-reactive CD8+ T cells display a restricted BV repertoire. Naive (top) or A/NT/60/68-pri
The exact cause of Guillain-Barré syndrome is unknown. It tends to appear after an infection and as a result of an immune response against nerve tissue. It appears that the immune system forms antibodies to target the antigens of the invading pathogen. These antibodies cross-react with nerve tissue and then directs the immune system to target this tissue. A number of different infections can trigger this immune-mediated response but infections are not the cause of the Guillain-Barré syndrome.. The immune system attacks the outer covering of the nerves known as the myelin sheath. This sheath is responsible for insulating the nerves. When this sheath is damaged it cannot transmit impulses as normal. As a result there is weakening, numbness or paralysis depending on which sensory (sensation) or motor (muscle) nerves are affected. However, in most cases this is not permanent and there is usually complete recovery. In a minority of cases, the weakening, numbness and paralysis may persist.. ...
|strong|Biotin conjugated Rabbit F(ab)|sub|2|/sub| anti Mouse IgG antibody|/strong| recognizes all subclasses of mouse IgG.|br||br|Cross reactivity with rat IgG is expected. Cross reactivity to huma…
TY - JOUR. T1 - Inflammatory co-morbidities in HIV+ individuals: learning lessons from healthy ageing. AU - Hearps, Anna Clare. AU - Martin, Genevieve. AU - Rajasuriar, Reena. AU - Crowe, Suzanne Mary. PY - 2014. Y1 - 2014. N2 - Increased life expectancy due to improved efficacy of cART has uncovered an increased risk of age-related morbidities in HIV+ individuals and catalyzed significant research into mechanisms driving these diseases. HIV infection increases the risk of non-communicable diseases common in the aged, including cardiovascular disease, neurocognitive decline, non-AIDS malignancies, osteoporosis, and frailty. These observations suggest that HIV accelerates immunological ageing, and there are many immunological similarities with the aged, including shortened telomeres, accumulation of senescent T cells and altered monocyte phenotype/function. However, the most critical similarity between HIV+ individuals and the elderly, which most likely underpins the heightened risk of ...
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StabilGuard® Immunoassay Stabilizer (BSA-Free) effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefit of the protein free formulation is the elimination of interference and cross reactivity within immunoassay applications. ...
By manipulating stem cells, scientists have found they can grow beating cardiac tissue in a petri dish. The cells self-organized to form microchamber ...
Citoxlab has experience with a wide variety of compounds and has a team of pathologists dedicated to running TCR and immunohistochemistry studies.
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... ist eine Methode, die die Menge eines Antigens in einer Probe mit hoher Empfindlichkeit messen kann. Prinzipiell kann jede biologisc...
Evidence for a Nonspecific Factor Interfering in the Radioimmunoassay of Somatoliberin-Like Immunoreactivity in Human Seminal ...
The potential of peptide mimics of V. cholerae lipopolysaccharide (LPS) to elicit cross-reactive immune responses against LPS was investigated, as an alternative approach towards the development of a cholera vaccine. Peptide mimics of two protective epitopes were identified. Peptide mimics were identified by screening phage display libraries with two closely related Ogawa serotype specific (monoclonal antibodies) mAbs, S-20-4 and A-20-6 and novel protective mAb 72.1 that cross-reacts with both Inaba and Ogawa serotypes. Six phage clones identified from the S-20-4 panning showed significant binding to both S-20-4 and A-20-6. Thus, it is likely that these phage displayed peptides mimic an important conformational epitope of Ogawa antigens and are not simply functionally recognized by S-20-4. In order to predict how Ogawa peptide mimics interact with S-20-4 compared to its carbohydrate counterpart, one peptide mimic, 4P-8, which is one of the highest affinity binders to anti-LPS Abs and shares ...
Extensive cross-reactivity among the different individual species of the genus could be expected. Extensive cross-reactivity occurs among various members of the Rosaceae family, but to date, the amount of cross-reactivity between strawberry and other members of the family has not been evaluated. (19). Fra a 1 has a 54-61% and a 77-78% sequence identity with Bet v 1 and the homologous apple allergen Mal d 1, respectively. (4, 5) Cross-reactivity with other plants containing a Bet v 1 homologue is therefore possible.. Allergy to Rosaceae fruit is frequently associated with birch pollinosis in central and northern Europe, and with grass pollen allergy in central Spain. The main cross-reactive structures involved in individuals who have birch pollen allergy are Bet v 1 and profilin; in grass pollen-allergic individuals, profilin and carbohydrate determinants. Rosaceae fruit allergy can occasionally occur in patients without pollinosis. Eleven patients from central Spain allergic to apples, peaches, ...
Rickettsioses are caused by species of Rickettsia, a genus comprising organisms characterized by their strictly intracellular location and their association with arthropods. Rickettsia species are difficult to cultivate in vitro and exhibit strong serological cross-reactions with each other. These technical difficulties long prohibited a detailed study of the rickettsiae, and it is only following the recent introduction of novel laboratory methods that progress in this field has been possible. In this review, we discuss the impact that these practical innovations have had on the study of rickettsiae. Prior to 1986, only eight rickettsioses were clinically recognized; however, in the last 10 years, an additional six have been discovered. We describe the different steps that resulted in the description of each new rickettsiosis and discuss the influence of factors as diverse as physicians curiosity and the adoption of molecular biology-based identification in helping to recognize these new ...
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Figure 6. Immunoblots demonstrating localization of PSA3 to the stromal face of the thylakoid membrane. Proteins were fractionated by SDS-PAGE, transferred to nitrocellulose, and probed with the indicated antibodies. An excerpt of an image of each blot stained with Ponceau S is shown to illustrate the total population of proteins in each lane and to serve as a loading control. The large subunit of Rubisco (RbcL) is marked. A, Immunoblot analysis of total leaf proteins from maize, demonstrating specificity of the PSA3 antibody. The size of the major protein detected in the wild type (WT; ∼26 kD) corresponds to that predicted for mature Zm-PSA3. Several cross-reacting proteins accumulate to elevated levels in the mutants. The identities of these proteins are not known. B, Relative PSA3 concentration in extracts of leaf, purified mitochondria (Mito), and purified chloroplasts (CP). A blot that had been used in a previous publication (Kroeger et al., 2009) was reprobed to detect PSA3. D1 (the PsbA ...
Abstract: A radioimmunoassay (RIA) of androstenedione in biological fluids was developed. The sensitivity of the method was 30 pg/ml. The efficiency of the standard detection in blood and saliva was 98.2%. The intra- and interassay variations were 5.6% and 12,7%, respectively. Specificity pf the antiserum was determined in cross reactions using 17 steroids. The specificity depended upon the method used, the duration of immunization and the quantity of the antigen. Mathematic methods were used for definition of optimal values of the main parameters and conditions for RIA. 60 different regimes were investigated. The influence of regular physical exercises on androstenedione content in blood and saliva was investigated in 100 athletes. Physical exercises increased androstenedione concentration in men and women blood and saliva. Concentration of androstenedione was increased 1.3- and 2.2-fold in men blood and saliva, respectively, the concentration of androstenedione was increased 1.6-fold in women ...
Enzyme immunoassay (EIA) drug screen test results, such as results reported in a Clinical Substance Abuse Panel (CSAP) or a Medical Urine Drug Screen or 810M, are screen only test results. "Positive" screen results may be due to other chemical cross reactions, and may not be indicative of substance abuse (e.g. amphetamine positive screen results may be caused by over the counter cold medications ...
In July I had two severe reactions to nutmeg. I thought it was to tree nuts, but I kept having more reactions. Blood tests were positive for Nutmeg, grass, dust, trees, cat and (peanut). Skin Tests to peanuts, tree nuts, seeds and a lot of other things were all negative. Did not skin test nutmeg, too scary. About six months ago almonds started making my lips tingle and swell slightly. My allergist says its a cross reaction to my pollen allergy. So Im avoiding tree nuts. In 2010 on a cruise, fresh nutmeg grated on tropical drinks made my lips swell up really badly. I took benadryl and that helped somewhat. Never thought of it again. Then in July I was on a trip overseas and had to be rushed to the hospital because my throat closed up when I had a small piece of baklava. Three days later, I ate a piece of chicken with Arabic spices, almost the same thing happened. The first time I was in a foreign country, the second reaction I was on a plane. Very traumatic. Had several more reactions in the ...
Objective: Although Chlamgdia pneumoniae is considered a common cause of pneumonia worldwide, the evidence is mainly serologic. Therefore, we examined whether the currently used chlamydial microimmunofluorescence MIF antibody test is specific for C pneumonoae infection. Design and setting: Secondary analysis of data from a cohort study of...
We have recently proposed a new model for antigenic variation in Plasmodium falciparum that relies on a network of partially cross-protective immune responses to orchestrate this complex immune evasion process. In addition to exhibiting prolonged oscillations of single variants that resemble the sequential dominance of immunologically distinct antigenic types, the model implies that a higher efficacy of cross-reactive immunity actually increases the length of infection while reducing severity of disease. Here, we analyse the behaviour of a reduced system under conditions of perfect synchrony between variants to demonstrate that these features of this system can be attributed to the antagonism between cross-reactive and variant-specific responses.
Human norovirus (NoV) strains cause a considerable number of outbreaks of gastroenteritis worldwide. Based on their capsid gene (VP1) sequence, human NoV strains can be grouped into two genogroups (GI and GII) and at least 14 GI and 17 GII genotypes (GI/1-14 and GII/1-17). Human NoV strains cannot be propagated in cell-culture systems, but expression of recombinant VP1 in insect cells results in the formation of virus-like particles (VLPs). In order to understand NoV antigenic relationships better, cross-reactivity among 26 different NoV VLPs was analysed. Phylogenetic analyses grouped these NoV strains into six GI and 12 GII genotypes. An antibody ELISA using polyclonal antisera raised against these VLPs was used to determine cross-reactivity. Antisera reacted strongly with homologous VLPs; however, a number of novel cross-reactivities among different genotypes was observed. For example, GI/11 antiserum showed a broad-range cross-reactivity, detecting two GI and 10 GII genotypes. Likewise, GII/1, GII
Rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) are chronic, debilitating autoimmune diseases predominately affecting women of reproductive age. Maternal exposure to specific child (foreign) antigens during pregnancy in women may influence risk of developing RA, SLE and related clinical outcomes. A better understanding of the relationship between exposure to non-self gene products that occurs during pregnancy will improve the understanding of autoimmune disease pathogenesis in women.. Autoimmune disorders (ADs) represent a major burden to the health care system and are a significant public health concern;as high as 10% of the population is affected by one or more ADs. The overall goal of this proposal is to investigate the role of maternal-child (fetal) genetics using several analytical approaches in two common ADs: rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Female predominance, onset during childbearing years, and immunologic similarities to ...
Findings could lead to improved vaccines for zoonotic diseases A collaborative study led by The Pirbright Institute has provided insights that could lead to the design of better vaccinations for animals and humans.. Swine flu, such as the H1N1 strain that was one of the strains that caused a pandemic in 2009, is an important pathogen in pig herds and can also be dangerous for humans.. Current vaccines are designed to protect against viruses of similar ancestry, so the control of infections where the virus may originate in a different species (i.e. birds) remains a challenge.. Previous research shows that pigs infected with avian-like H1N1 influenza lineage are able to induce complete protection against both the strain and the 2009 pandemic lineage. It also found that protection was conferred, despite the absence of cross-reactive antibodies - suggesting the involvement of cross-reactive T-cells.. To investigate this further, a team led by Dr Maria Montoya, a specialist in porcine immunology at ...
This website is intended as an informational guide. The described approaches and suggested therapies are meant to complement, not replace, professional medical advice and treatment. You are encouraged to seek advice from your doctor on matters related to MS ...
Peptides that combine poorly to MHC course We substances elicit low functional avidity Capital t cell reactions often. immune system reactions can become increased towards Capital t cell epitopes with low practical avidity by raising antigen denseness. We also determined a heteroclitic epitope (RCVIFANI) that elicited a Capital t cell response with almost full cross-reactivity with indigenous epitope and proven improved MHC-peptide plethora likened to indigenous S i9000598. Structural and thermal dissolve studies indicated that the Queen600V replacement improved balance of the peptide-MHC complicated without significantly changing the antigenic surface area, causing in cross-reactive Big t cell reactions extremely. Our data high light that improved pMHC Rabbit polyclonal to IL25 complicated screen contributes to heteroclitic epitope effectiveness and explain guidelines for increasing immune system reactions that cross-react with the indigenous epitope. Intro growth and Virus distance both ...
Hello, Your situation reactive ELISA, indeterminate Western Blot during or after pregnancy is not uncommon. See below. The most common cause is cross-reacting antibodies resulting form the...
... (Anti-Human Gold Conjugates). Affinity isolated anti-human IgA, IgG, IgM (H+L) antibody produced in goat and coupled to 20nm gold nanoparticles (1ml, OD3). Pre-adsorbed with mouse serum for reduced cross reactivity.
... (Anti-Human Gold Conjugates). Affinity isolated anti-human IgA, IgG, IgM (H+L) antibody produced in goat and coupled to 15nm gold nanoparticles (1ml, OD3). Pre-adsorbed with mouse serum for reduced cross reactivity.
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The measurement of chemicals and their metabolites in the blood for biological monitoring of exposure was discussed. As the blood is the transport vehicle for chemicals and their metabolites in the body, most determinants present in the body can be found in measurements of the blood. The advantages of blood monitoring include the fact that relatively small interindividual variations of those blood
Homology in the 16S rDNAs shows that the agent of human granulocytic ehrlichiosis (HGE) is closely related to the veterinary pathogens Erlichia equi and Erlichia phagocytophila. After HGE, patients develop antibodies reactive with E. equi and E. phagocytophila; thus, we hypothesized that these species are closely related and share significant antigenicity. Antisera from humans, horses, dogs, and cattle were tested by indirect fluorescent-antibody assay (IFA) for antibodies reactive with E. equi and other ehrlichiae and tested by immunoblot to identify the specific reactions with E. equi. All convalescent-phase sera from human patients with HGE and from animals infected or immunized with E. equi or E. phagocytophila had antibodies reactive with E. equi by IFA; no reactions with Ehrlichia chaffeensis occurred with these sera, and only one horse naturally infected with E. equi had a serologic reaction against Ehrlichia sennetsu. Human and animal sera obtained after infection or immunization with ...
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Factor H binding protein (fHbp) is one of the main antigens of the 4-component meningococcus B (4CMenB) multicomponent vaccine against disease caused by serogroup B Neisseria meningitidis (MenB). fHbp binds the complement down-regulating protein human factor H (hfH), thus resulting in immune evasion. fHbp exists in 3 variant groups with limited cross-protective responses. Previous studies have described the generation of monoclonal antibodies (mAbs) targeting variant-specific regions of fHbp. Here we report for the first time the functional characterization of two mAbs that recognize a wide panel of fHbp variants and subvariants on the MenB surface and that are able to inhibit fHbp binding to hfH. The antigenic regions targeted by the two mAbs were accurately mapped by hydrogen-deuterium exchange mass spectrometry (HDX-MS), revealing partially overlapping epitopes on the N terminus of fHbp. Furthermore, while none of the mAbs had bactericidal activity on its own, a synergistic effect was observed for
Several viruses in the Arenaviridae family infect humans and cause severe hemorrhagic fevers which lead to high case fatality rates. Due to their pathogenicity and geographic tropisms, these viruses remain very understudied. As a result, an effective vaccine or therapy is urgently needed. Here, we describe efforts to produce cross-reactive monoclonal antibodies that bind to both New and Old World arenaviruses. All of our MAbs seem to be nonneutralizing and nonprotective and target subunit 2 of the glycoprotein. Due to the lack of reagents such as recombinant glycoproteins and antibodies for rapid detection assays, our MAbs could be beneficial as analytic and diagnostic tools.
In Europe, many flaviviruses are endemic (West Nile, Usutu, tick-borne encephalitis viruses) or occasionally imported (dengue, yellow fever viruses). Due to the temporal and geographical co-circulation of flaviviruses in Europe, flavivirus differentiation by diagnostic tests is crucial in the adaptation of surveillance and control efforts. Serological diagnosis of flavivirus infections is complicated by the antigenic similarities among the Flavivirus genus. Indeed, most flavivirus antibodies are directed against the highly immunogenic envelope protein, which contains both flavivirus cross-reactive and virus-specific epitopes. Serological assay results should thus be interpreted with care and confirmed by comparative neutralization tests using a panel of viruses known to circulate in Europe. However, antibody cross-reactivity could be advantageous in efforts to control emerging flaviviruses because it ensures partial cross-protection. In contrast, it might also facilitate subsequent diseases, through a
PAULIN, Lília Márcia Silva et al. Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis). Cienc. Rural [online]. 2012, vol.42, n.9, pp.1621-1626. Epub 14-Ago-2012. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782012005000070.. The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also ...
Cross-reaction toxicity[edit]. Supplemental zinc can prevent iron absorption, leading to iron deficiency and possible ...
"Cross-Coupling Reactions". Encyclopedia of Reagents for Organic Synthesis. doi:10.1002/047084289X.rd100.pub2. Ljungdahl, Thomas ... "Two Competing Mechanisms for the Copper-Free Sonogashira Cross-Coupling Reaction". Organometallics. 27 (11): 2490-2498. doi: ... in an effective catalyst for coupling reactions such as the Kumada coupling and Suzuki reactions (example below). It also ... Kumada, Makota; Tamao, Kohei; Sumitani, Koji (1988). "Phosphine-Nickel Complex Catalyzed Cross-Coupling of Grignard Reagents ...
These enzymes are capable of carry out reaction even in the absence of the protein-based active site organization or carry out ... X. Cross-association of Tert Butyllithium. Kinetics of Tert Butyllithium Dissociation". Journal of the American Chemical ... Her work focused on the kinetic studies of organolithium reactions. Darensbourg received a B.S. in Chemistry from Union College ... According to Darensbourg, The ligands can act as a catalyst to carry out different reactions, depending on which transition ...
Reaction mechanism[edit]. The reaction mechanism is thought to proceed via a standard Pd catalyzed cross-coupling pathway, ... The Negishi coupling is a widely employed transition metal catalyzed cross-coupling reaction. The reaction couples organic ... Sase, S.; Jaric, M.; Metzger, A.; Malakhov, V.; Knochel, P. (2008). "One-Pot Negishi Cross-Coupling Reactions of In Situ ... Nicolaou, K. C.; Bulger, P. G.; Sarlah, D. (2005). "Palladium-catalyzed cross-coupling reactions in total synthesis". Angew. ...
... the Kumada coupling is a type of cross coupling reaction, useful for generating carbon-carbon bonds by the reaction of a ... Heck reaction Hiyama coupling Suzuki reaction Negishi coupling Petasis reaction Stille reaction Sonogashira coupling Corriu, R ... The reaction is notable for being among the first reported catalytic cross-coupling methods. Despite the subsequent development ... Frisch, Anja C.; Beller, Matthias (21 January 2005). "Catalysts for Cross-Coupling Reactions with Non-activated Alkyl Halides ...
Many decades later, interest in base metal catalysis sparked significant efforts to further develop cross-coupling reactions ... Fürstner, Alois; Leitner, Andreas; Méndez, María; Krause, Helga (2002-11-01). "Iron-Catalyzed Cross-Coupling Reactions". ... He developed the Kochi reaction, a variation on the Hunsdiecker reaction. Kochi was of Japanese descent and he and his family ... Kochi's work showed that this reaction proceeds via a complex between benzene (the donor) and nitrosonium ion (the acceptor). ...
These hypersensitivity reactions to tetrazepam share no cross-reactivity with other benzodiazepines. Tetrazepam is used ... Barbaud, A; Girault, PY; Schmutz, JL; Weber-Muller, F; Trechot, P (July 2009). "No cross-reactions between tetrazepam and other ... Kämpgen, E; Bürger, T; Bröcker, EB; Klein, CE (November 1995). "Cross-reactive type IV hypersensitivity reactions to ... Allergic reactions to tetrazepam occasionally occur involving the skin. Allergic reactions can develop to tetrazepam and it is ...
88, 860 (1952). Cross Sections for Nuclear Reactions Involving Nuclear Isomers. Bruno Linder* and Ralph A. James. Department of ... Vol 93, Number 2, p. 288-290 Proton Induced Reactions of Thorium-Fission Yield Curves. Howard A. Tewes and Ralph A. James. ... 85, 369 (1952). Excitation Functions of Proton-Induced Reactions of Nb93. Ralph A. James. Physical Review - January 1954. ...
... reactions; interaction cross sections for Lithium and the production of undesirable particles; interaction cross sections of ... Cross Section of D (d, n) He-3 Reaction. Coon & Graves. Hydrogen Recoil Proportional Counter for Neutron Detection. The ... Manley, J. H., Coon, J. H., & Graves, Elizabeth R. (January 1946). Cross Section of D (D, N) He-3 Reaction. PHYSICAL REVIEW. 70 ... Total Cross Sections of Tritium, Hydrogen, and Helium for Fast Neutrons. Total Cross Sections for 14 MeV Neutrons. Elastic ...
世界の化学者データベース「鈴木章」 Miyaura, Norio; Suzuki, Akira (1995). "Palladium-Catalyzed Cross-Coupling Reactions of Organoboron Compounds ... "A new stereospecific cross-coupling by the palladium-catalyzed reaction of 1-alkenylboranes with 1-alkenyl or 1-alkynyl halides ... who first published the Suzuki reaction, the organic reaction of an aryl- or vinyl-boronic acid with an aryl- or vinyl-halide ... The postdoctoral experience was utilized in the study of the coupling reaction with his assistant Norio Miyaura and led to the ...
These products are useful for cross-coupling reactions. (9) Zirconium-catalyzed carboalumination of alkynes by trimethylalane ... Workup of these reactions should employ extreme pH's (10% hydrochloric acid or 6 N sodium hydroxide), as moderate pH encourages ... Reactions of alkenyl- and alkynylaluminium compounds involve the transfer of a nucleophilic alkenyl or alkynyl group attached ... House, H. O. Modern Synthetic Reactions, 2nd ed., Benjamin/Cummings, Menlo Park, CA, 1972. Eisch, J. J.; Kaska, W. C. J. Am. ...
doi:10.1016/S0040-4039(00)60957-7. Miyaura, Norio; Suzuki, Akira (1995). "Palladium-Catalyzed Cross-Coupling Reactions of ... 2005). Name Reactions and Reagents in Organic Synthesis (2nd ed.). ISBN 0471228540. Comins, Daniel L.; Dehghani, Ali (1992). " ... The vinyl triflates prepared are useful as substrates in the Suzuki reaction. Mundy, Bradford P.; Ellerd, Michael G.; Favaloro ...
These versatile intermediates can then be used for a wide range of chemical transformations such as cross-coupling reactions, ... The reaction is mechanistically related to the Tebbe reaction and can be catalyzed by various Lewis acids (e.g. TiCl4 or Al2Me6 ... This powerful carbon-carbon bond forming cross-coupling reactions combines an organic halide and an organozinc halide reagent ... This group transfer reaction can be used in allylation, or other coupling reactions (such as Negishi coupling). One of the ...
"Palladium-Catalyzed Cross-Coupling Reactions of Organoboron Compounds". Chemical Reviews. 95 (7): 2457-2483. doi:10.1021/ ... This reaction type is exemplified in the Suzuki reaction, which involves coupling of aryl- or vinyl-boronic acid with an aryl- ... A common reagent in this type of reaction is bis(pinacolato)diboron. In organic synthesis the hydroboration reaction is taken ... Compounds of carbon with other elements in the periodic table: Displayed is a reaction sequence starting with reaction of allyl ...
D. Enders, O. Niemeier & T. Balensiefer (2006). "Asymmetric Intramolecular Crossed-Benzoin Reactions by N-Heterocyclic Carbene ... and the reaction mechanism for this organic reaction was proposed in 1903 by A. J. Lapworth. In the first step in this reaction ... The reaction is catalyzed by a nucleophile such as the cyanide anion or an N-heterocyclic carbene. The reaction product is an ... The reaction can be extended to aliphatic aldehydes with base catalysis in the presence of thiazolium salts; the reaction ...
Diederich, F.; de Meijere, A. (Eds.) "Metal-Catalyzed Cross-Coupling Reactions II", Wiley-VCH, Weinheim, 2004. Diederich, F.; ... Diederich, F.; Stang, P. J. (Eds.) "Metal-Catalyzed Cross-Coupling Reactions", Wiley-VCH, Weinheim, 1998. Diederich, F.; Stang ...
Cross-reactions may occur between mango allergens and urushiol. During the primary ripening season of mangoes, contact with ... Oka K, Saito F, Yasuhara T, Sugimoto A (2004). "A study of cross-reactions between mango contact allergens and urushiol". ... Miell J, Papouchado M, Marshall A (1988). "Anaphylactic reaction after eating a mango". British Medical Journal. 297 (6664): ...
Illustrating the Sonogashira cross coupling reactions is in the synthesis of imidazopyridine derivatives. A general mechanism ... In an example of a present-day cross coupling reaction called decarboxylative coupling, a catalytic amount of Cu(I) displaces a ... Beletkaya, I.P.; Cheprakov, A.V. (2004). "Copper in Cross Coupling Reactions: The Post Ullman Chemistry". Coord. Chem. Rev. 248 ... Prior to the development of palladium-catalyzed cross coupling reactions, copper was the preferred catalyst for almost a ...
Cross-allergic reactions occur among the β-lactam antibiotics. To determine whether treatment with a β-lactam is safe when an ... Approximately five percent of patients have some kind of reaction, ranging from maculopapular rash (the most common rash seen ... which reacts with proteins and serves as a hapten to cause an immune reaction. ... allergy is noted, patient history regarding severity of previous reaction is essential. Ferrier, Denise R. (2011). Pharmacology ...
Pathology to observe Blood grouping, Haematology & cross matching reactions. It also involves PRO department for the ... and cross matches units that are negative for the antigen. Urinalysis tests urine for many analytes. Some health care providers ...
Cross-section of part of the planned ITER fusion reaction vessel.. The vacuum vessel is the central part of the ITER machine: a ... There are several reactions that produce tritium within the blanket. 6. Li. produces tritium via n,t reactions with moderated ... A heuristic for estimating reaction rates is that nuclei must be able to get within 100 femtometers (1 × 10−13 meter) of each ... Refine neutron shield/heat conversion technology (most of the energy in the D+T fusion reaction is released in the form of fast ...
Miyaura, Norio; Suzuki, Akira (1979). "Palladium-Catalyzed Cross-Coupling Reactions of Organoboron Compounds". Chemical reviews ... Suzuki, Akira (1991). "Synthetic Studies via the cross-coupling reaction of organoboron derivatives with organic halides". Pure ... Miyaura, Norio; Yamada, Kinji ; Suzuki, Akira (1979). "A new stereospecific cross-coupling by the palladium-catalyzed reaction ... Miyaura, Norio; Suzuki, Akira (1995). "Palladium-Catalyzed Cross-Coupling Reactions of Organoboron Compounds". Chemical Reviews ...
"Mechanistic Duality in Palladium-Catalyzed Cross-Coupling Reactions of Aryldimethylsilanolates. Intermediacy of an 8-Si-4 ... Ogawa, Akiya; Curran, Dennis P. "Benzotrifluoride: A Useful Alternative Solvent for Organic Reactions Currently Conducted in ... and silylation reactions. The dielectric constants for dichloromethane and trifluorotoluene are 9.04 and 9.18, respectively, ... trifluorotoluene is useful in mild Lewis-acid catalyzed reactions, such as the Friedel-Crafts preparations. The most common ...
... s are also starting materials for certain cross-coupling reactions. In the first report on this reaction type the ... This reaction requires a strong base. If sodium methoxide is used as the base the reaction is called a Bamford-Stevens reaction ... In the Shapiro reaction tosylhydrazones are used as a leaving group in elimination reactions. ... New Uses for Classic Reagents in Palladium-Catalyzed Cross-Coupling and Metal-Free Reactions. Angewandte Chemie International ...
The UV radiation drives the polymerization and cross-linking reactions. The resulting "logs" are sticky gels containing 60-70% ... Additional cross-linking agent may be sprayed on the particles' surface; this "surface cross-linking" increases the product's ... cross-linked carboxymethylcellulose, polyvinyl alcohol copolymers, cross-linked polyethylene oxide, and starch grafted ... Low-density cross-linked SAPs generally have a higher absorbent capacity and swell to a larger degree. These types of SAPs also ...
Northrup, A. B.; MacMillan, D. W. C. (2002). "The First Direct and Enantioselective Cross-Aldol Reaction of Aldehydes". Journal ... Additional reactions include aldol reactions, Mannich reaction, Michael reaction, amination, α-oxyamination, and α-halogenation ... Marques, M. M. B. (2006). "Catalytic Enantioselective Cross-Mannich Reaction of Aldehydes". Angewandte Chemie International ... In the Hajos-Parrish-Eder-Sauer-Wiechert reaction, proline catalyses an asymmetric aldol reaction. The zwitterionic character ...
... the mass of any one element at the beginning of a reaction will equal the mass of that element at the end of the reaction.[24]: ... This has led to the concept or area of study known as cross-boundary subsidy.[59][60] "This leads to anomalies, such as food ... These chemical reactions require energy, which mainly comes from the Sun and largely by photosynthesis, although a very small ... The components of food webs, including organisms and mineral nutrients, cross the thresholds of ecosystem boundaries. ...
"A Highly Active Catalyst for Pd-Catalyzed Amination Reactions: Cross-Coupling Reactions Using Aryl Mesylates and the Highly ... Martin, Ruben; Buchwald, Stephen L. (18 November 2008). "Palladium-Catalyzed Suzuki−Miyaura Cross-Coupling Reactions Employing ... Martin, Ruben; Buchwald, Stephen L. (18 November 2008). "Palladium-Catalyzed Suzuki−Miyaura Cross-Coupling Reactions Employing ... Miyaura Cross-Coupling Reactions of C(sp2)-Tosylates and Mesylates". Organic Letters. 11 (17): 3954-3957. doi:10.1021/ol9015892 ...
Also, IgE-tests can be false positive due to sensitization without symptoms, tolerance-development or cross-reactions[12, 31, ... Birch-pollen allergy and cross-reactions to birch-like proteins in vegetables are common in the Swedish population[31]. Others ... especially oral reactions to fruit and gastrointestinal reactions to milk. Acta Paediatr. 2014, Epub 2014/08/12 ... Soost S, Leynaert B, Almqvist C, Edenharter G, Zuberbier T, Worm M: Risk factors of adverse reactions to food in German adults ...
American Red american red cross hygiene kits Cross Woodward Detroit with the much more total ... individuals reactions at the surgical clients demand not less than to speak for their industry the 2 nurses who I wager will be ... American Red american red cross hygiene kits Cross Woodward Detroit with the much more total ensemble in trying to find the ... American Red Cross Woodward Detroit. Published August 31, 2011 , By admin. Nevertheless some standard factors that wishes to ...
... written for Red Cross staff by Dr. B.P.L. (Paddy) Moore (and others), National Director of the Red Cross Blood Group Reference ... An ABO incompatible red cell unit was transfused resulting in a haemolytic transfusion reaction. The blood was issued using an ... Lo and behold - a friend said, Pat, Canadian Red Cross Blood Transfusion Service hires BSc grads because most med lab tech ... see these articles from 45 years ago by Canadian Red Cross staff, including Dr. B.P.L. Moore. The second includes, The ...
The traditional transition metal-catalyzed cross-coupling reaction, although well suited for C(sp2)-C(sp2) cross-coupling, has ... Ni and Fe-Based Cross Coupling Reactions Articles in Focus. Enjoy free access to selected articles from our recently completed ... Article 2: Fe-Catalyzed Cross-Dehydrogenative Coupling Reactions. by Leiyang Lv, Zhiping Li ... Cross-dehydrogenative coupling (CDC), which enables the formation of carbon-carbon (C-C) and C-heteroatom bonds from the direct ...
About the American Red Cross: The American Red Cross shelters, feeds and provides emotional support to victims of disasters; ... The Red Cross is a not-for-profit organization that depends on volunteers and the generosity of the American public to perform ... Its important to be prepared in case of a reaction, and were excited to offer this class to the community. This is a great ... "Its important to be prepared in case of a reaction, and were excited to offer this class to the community," said Kelly Hudson ...
Cross-national evidence of a negativity bias in psychophysiological reactions to news. View ORCID ProfileStuart Soroka, Patrick ... Replication Data for: Cross-National Evidence of a Negativity Bias in Psychophysiological Reactions to News. Harvard Dataverse ... Cross-national evidence of a negativity bias in psychophysiological reactions to news ... Cross-national evidence of a negativity bias in psychophysiological reactions to news ...
The surprise is how many of the reactions crossed the usual political boundaries. ... A Breast-Feeding Plan Mixes Partisan Reactions. By KATE ZERNIKE. FEB. 17, 2011. ... A Breast-Feeding Plan Mixes Partisan Reactions. Order Reprints, Todays Paper,Subscribe ...
Negishi cross-coupling reactions of secondary alkyl zinc halides with aryl halides utilize PEPPSI™-IPent, boasting higher ... In Stille-Migita cross-couplings of heteroaryl stannanes with aryl halides, PEPPSI™-IPent allowed for lower reaction ... The PEPPSI™-IPent (732117) further advances the agility of cross-coupling reactions by being air-stable and thus increasing ... IPent is a highly effective catalyst in challenging Suzuki-Miyaura and Negishi cross-coupling reactions. This produces tetra- ...
... to set up and troubleshoot some of the most commonly used cross-coupling reactions throughout academia and industry.. The Cross ... Setting Up a Cross-Coupling Reaction. General Troubleshooting. Buchwald-Hartwig Amination:. Primary & Secondary Amines. ... Claim Your Complimentary Cross-Coupling Reaction Guide. Please provide the required (*) information below to your free copy of ... with the Cross-Coupling Guide. We are excited to introduce our new Cross-Coupling Guide to support practicing chemists like you ...
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Idiotypic cross-reactions of monoclonal human lupus autoantibodies.. Y Shoenfeld, D A Isenberg, J Rauch, M P Madaio, B D ... Idiotypic cross-reactions of monoclonal human lupus autoantibodies.. Y Shoenfeld, D A Isenberg, J Rauch, M P Madaio, B D ... The idiotypic cross-reactions of immunoglobulins from unrelated patients suggest that the autoantibodies are derived from ... Idiotypic cross-reactions were evaluated in 60 polynucleotide-binding monoclonal lupus autoantibodies produced by human-human ...
CHICAGO -- Heres a quick look at the Chicago Blackhawks 4-3 triple-overtime win over the Boston Bruins in Game 1 of the Stanley Cup finals at the United Center on Wednesday.
Red Cross Tents At Yunnan Earthquake Site, Netizen Reactions. by Peter Barefoot Friday, September 14, 2012, 5:45 pm. 216 ... Those are not the Red Cross Societys tents. The Red Cross takes the money from donors, takes a percentage, and simply does ... What do you think? Of the Red Crosss work in this disaster? Of the netizen publics cynicism towards the Red Cross? ... The Red Cross Society cannot be forgiven, dont think you can put up a few tents and say youre doing good deeds. The money was ...
The neutron incident energy around 14-15 MeV is adequate to excite the nucleus for the reactions such as (n, p), (n,... ... n, α) cross section Nuclear reactions TALYS 1.9 nuclear model code EMPIRE 3.2 (Malta) nuclear model code ... Theoretical cross-sectional calculation of some structural fusion material on (n, α)-induced reactions. ... the reaction cross-sectional data have a critical importance in fusion reactors and development. Neutron irradiation produces ...
... highly chemoselective crossed acyloin condensation reactions between aldehydes and α-ketoesters to afford densely ... NHC-catalysed, chemoselective crossed-acyloin reactions Christopher A. Rose,a Sivaji Gundala,b Claire-Louise Fagan,b Johannes F ... NHC-catalysed, chemoselective crossed-acyloin reactions C. A. Rose, S. Gundala, C. Fagan, J. F. Franz, S. J. Connon and K. ... promote (at low loadings in the presence of base) highly chemoselective crossed acyloin condensation reactions between ...
Cross-Reactions of Streptococcal Group-Specific Substances in Antipneumococcal Serums Message Subject. (Your Name) has ... Cross-Reactions of Streptococcal Group-Specific Substances in Antipneumococcal Serums. By Michael Heidelberger, Richard M. ... Cross-Reactions of Streptococcal Group-Specific Substances in Antipneumococcal Serums. By Michael Heidelberger, Richard M. ...
Sonogashira reaction turned out to be the most effective and convenient method to introduce functionalized alkynyl group into ... In this way the utility of such reactions to heterocyclic systems, which were neglected so far, could be figured out. Halogen ... The introduction of the desired side chains by Carbon-Carbon bond formation reactions was achieved by Sonogashira coupling and ... As a synthetic strategy, palladium-catalyzed coupling reactions were used to introduce side chains and aryl substituents into ...
Although this reaction is catalysed by Ni or Pd complexes, the latter have been much more developed. Nevertheless, Ni ... The Negishi cross-coupling of organozinc reagents is a valuable tool for the formation of C-C bonds in the presence of ... Negishi cross-coupling. reactions: scope and mechanisms V. B. Phapale and D. J. Cárdenas, Chem. Soc. Rev., 2009, 38, 1598 DOI: ... Negishi cross-coupling. of organozinc reagents is a valuable tool for the formation of C-C bonds in the presence of functional ...
... Author(s). McAvoy, Camille Z ... Furthermore, the products of this cross-coupling can be used for a subsequent electrocyclization through a reaction with ... A final C-N bond formation study focuses on the cross-coupling of aryl halides with amidine salts. For this cross-coupling, a ... Thus, methodologies for various palladium-catalyzed, C-N cross-couplings as well as a potential ligand synthesis to be used for ...
For others, the allergic reaction to an insect sting can be life-threatening. ... the reaction to a sting is brief, with redness and swelling followed by pain and itching. ... Sometimes this reaction can be life-threatening.. This severe reaction is a medical emergency that can happen very quickly. It ... The reaction is brief, with redness and swelling followed by pain and itching. Generally, the reaction lasts only a few hours, ...
... and reduced cross sections [equation]allows the comparison of heavy-ion-induced reaction cross sections for a broad range of... ... Angular Distribution Excitation Function Reaction Cross Section Compound Nucleus Coulomb Barrier These keywords were added by ... Cross sections of α-induced reactions for targets with masses A ≈ 20-50 at low energies. ... Motivated by these unexpected discrepancies, the present study analyses \( \alpha\)-induced reaction cross sections for targets ...
... Ya- ... production cross sections in interactions of charged pions on targets at high momentums are analyzed by using a multicomponent ...
... Ian C. Stewart, Christopher J. Douglas and ... Steric bulk of N-heterocyclic carbene ligands affects the efficiency in cross-metathesis reactions. For the formation of ...
The Reductive Cross-Aldol Reaction. A reductive cross-aldol reaction was recently developed by the Baba group (Org. Lett. 2005 ... and the reductive cross-aldol reaction.. II. The Cross-Aldol reaction of aldehyde enolates with aldehydes. The work developed ... Asymmetric Cross-Aldol Reactions Of Aldehydes: A Formidable Synthetic Challenge. The aldol addition reaction is one of the most ... Chem. 1980, 45, 1066). The cross-aldol reaction between an aldehyde and an α,β-unsaturated aldehyde via titanium enolates was ...
  • We hypothesized that reported FHS would be common and that over this age-range, reported FHS to cow´s milk and hen´s egg would decrease while there would be an increase of reported symptoms to foods containing birch-pollen cross-reacting proteins. (biomedcentral.com)
  • article{1315692, abstract = {The helicity dependence of the total cross-section for the gamma n -{\textrangle} p pi(-)pi(0) reaction has been measured for the first time at incident photon energies from 450 to 800MeV. (ugent.be)
  • ENGLISH ABSTRACT: The (p, pα) reaction on 12C was investigated experimentally using polarized incident protons of 100 MeV. (sun.ac.za)
  • The present study describes several novel 2,5-biaryl-3-hexylthiophene derivatives ( 3a - i ) synthesized via a Pd(0)-catalyzed Suzuki cross-coupling reaction in moderate to good yields. (mdpi.com)
  • These composite catalysts also mediated the cross-coupling Suzuki reaction providing the expected products in quantitative yields under relatively mild conditions after 4 h at 50 ºC. (scielo.br)
  • The thick target yield of the reaction D + D yields T + p + 3.98 Mev has been measured, using a heavy ice target, and observations have been made on the angular distribution of the protons. (dtic.mil)
  • The complex 10 (1.5 mmol %) was utilized in Suzuki-Miyaura cross-coupling reaction to prepare substituted biphenyls, terphenyls, and heterobiaryls in good to excellent yields. (banglajol.info)
  • The advantages of the protocol are its generality{,} high yields{,} short reaction time{,} a cleaner reaction profile{,} and simplicity. (ubc.ca)
  • The traditional transition metal-catalyzed cross-coupling reaction, although well suited for C(sp2)-C(sp2) cross-coupling, has proven less amenable toward coupling of C(sp3)-hybridized centers, particularly using functional group tolerant reagents and reaction conditions. (springer.com)
  • The Negishi cross-coupling of organozinc reagents is a valuable tool for the formation of C-C bonds in the presence of functional groups. (rsc.org)
  • The substrate scope of the MnCl 2 -catalyzed cross-coupling between aryl halides and Grignard reagents has been extended to several methyl-substituted aryl iodides by performing the reaction at elevated temperature in a microwave oven. (dtu.dk)
  • From your measurements, what are the reaction orders with respect. (transtutors.com)
  • Electron paramagnetic resonance measurements provided evidence for the formation of superoxide radical anions (O2−⋅) rather than singlet oxygen (1O2) during these photocatalytic reactions. (hku.hk)
  • article{osti_1375973, title = {Cross-section measurements for the Fe 57 ( n , n γ ) Fe 57 and Fe 57 ( n , 2 n γ ) Fe 56 reactions}, author = {Negret, A. and Sin, M. and Borcea, C. and Capote, R. and Dessagne, Ph. (osti.gov)
  • The scope of the process is extraordinarily broad with respect to both coupling partners, and a preliminary study has established the principle that a high degree of stereochemical control over the reaction can also be exercised via the use of a chiral NHC precursor. (rsc.org)
  • The scope of the BrettPhos precatalyst for the cross-coupling of ortho-substituted aryl iodides with amides is studied using substrates with a variety of functional groups. (mit.edu)
  • The results showed that Iso10 and S. boydii cross- reacted but no cross-reaction could be detected between E. fergusonii and Shigella- anitsera, which indicates a previous horizontal gene transfer of the O- antigen gene cluster (rfb) between S. boydii 15 and Iso10. (uib.no)
  • For this reason, bulky, monodentate phosphine ligands encourage reductive elimination and have emerged as a popular ligand class for these reactions. (organicreactions.org)
  • Molecular characterization of an environmental bacterial isolate with strong serological cross-reaction with Shigella boydii 15. (uib.no)
  • This work presents results of crossed molecular beam imaging studies on the reaction of radicals (Cl and CN) with polyatomic hydrocarbons of different functionalities such as pentane isomers, deuterated alkanes, alkenes, and alcohols. (wayne.edu)
  • Current molecular research is providing helpful information in the prediction of cross-reactions. (foodintolerances.org)
  • Among chemical processes, attention is given to ion-molecule reactions, dissociative recombination of molecular ions, and reactions between neutral species. (wright.edu)
  • Compared to native collagen, methionine (Met) and hydroxylysine (Hylys) decreased 60% and 20%, respectively, and both tyrosine (Tyr) and histidine (His) were no longer detected at all in cross-linked collagen type I. After cross-linking, collagen was less well digested into peptides by cyanogen bromide, but was more easily digested by collagenase than was native collagen. (arvojournals.org)
  • Purified a-Ro60 antibodies reacted with both peptides and inhibition experiments revealed the specificity of this reaction. (biomedcentral.com)
  • This study was aimed at analyzing whether lymphocytes of patients with VKH disease have a cross-reaction between melanocyte peptides and virus antigens. (arvojournals.org)
  • The mechanisms involved in Ni-catalysed reactions are different from the usual catalytic cycle involving oxidative addition-transmetalation-reductive elimination that is generally accepted for Pd-catalysed reactions. (rsc.org)
  • Chabon: These two available designers who both know download oxidative cross-coupling and both are different and both are a InsteadTechnical image to be themselves, help into a routine of information with each such. (myocept.com)
  • The rate of oxidative addition is typically independent of the nucleophile, and cross-couplings with TFBs are no exception. (organicreactions.org)
  • Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic Ehrlichia. (asm.org)
  • no reactions with Ehrlichia chaffeensis occurred with these sera, and only one horse naturally infected with E. equi had a serologic reaction against Ehrlichia sennetsu. (asm.org)
  • An example of the confusing serologic reactions which sometimes are obtained may be of interest: A young man developed undulant fever, with the date of onset in June 1936. (jamanetwork.com)
  • It has been shown for the first time that relatively electron deficient triazolium pre- catalysts promote (at low loadings in the presence of base) highly chemoselective crossed acyloin condensation reactions between aldehydes and α-ketoesters to afford densely functionalized products incorporating a quaternary stereocentre of considerable synthetic potential. (rsc.org)
  • This highlight focuses on developments concerning the cross-aldol reactions of aldehydes. (organic-chemistry.org)
  • Scheme 1 - Problems associated with metal-catalysed aldol reactions of aldehydes ( PNAS 2004 , 101 , 5439. (organic-chemistry.org)
  • Several problems are associated with aldol reactions of aldehydes due to the fact they suffer from undesired reactions, such as polyaldolisation (secondary additions to the aldehyde products), oligomerisation of the product (propensity of aldehydes to polymerise under metal-catalysed conditions), dehydration of the product and Tishchenko-type processes (Scheme 1). (organic-chemistry.org)
  • Scheme 2 - Cross-Aldol condensation of aldehydes using a combined reagent of Ti(O- n -Bu) 4 /t-BuOK (1:1). (organic-chemistry.org)
  • The results were validated by comparison with the well-known excitation function of the 64 Ni(p, n) 64 Cu reaction. (degruyter.com)
  • This tutorial review presents the more recent advances in Ni-catalysed Negishi cross-couplings for the formation of different kinds of C-C bonds and discusses their proposed reaction mechanisms. (rsc.org)
  • Devise a model reaction system to study biochemical mechanisms of collagen cross-linking. (arvojournals.org)
  • Purified collagen types I, III and IV were used to assess the mechanisms of cross-linking induced by the photosensitizer riboflavin (RF) and ultraviolet A (UVA) under conditions which resemble those for the clinical treatment of progressive keratoconus. (arvojournals.org)
  • We attempted to determine whether these symptoms could be due to either cross-contamination with gluten-containing foods or cross-reactivity between α-gliadin and non-gluten foods consumed on a GFD. (scirp.org)
  • To investigate whether there was cross-reactivity between α-gliadin antibody and different tissue antigens, we measured the degree to which this antibody bound to these antigens. (scirp.org)
  • We also observed significant cross-reactivity between α-gliadin 33-mer and various food antigens, but some of these reactions were associated with the contamination of non-gluten foods with traces of gluten. (scirp.org)
  • The lack of response of some CD patients may also be due to antibody cross-reactivity with non-gliadin foods. (scirp.org)
  • The lack of improvement in histopathology and clinical symptomatology in a subgroup of patients on a GFD may be associated with dietary non-adherence or cross-reactive epitopes triggering a state of heightened immunological reactivity in gluten-sensitive individuals . (scirp.org)
  • Cross-reactivity or cross-allergy is an immune-mediated phenomenon of IgE antibodies recognizing, binding, and inducing immune responses to molecules with similar structures. (foodintolerances.org)
  • Cross-reactivity between shellfish and house mite allergy is quite common. (foodintolerances.org)
  • Antibody F170-10 is fairly specific for artelinic acid but does bind artemisinin and artemether (3-5% cross-reactivity). (isharonline.org)
  • Through these years he has worked in several topics such as hypervalent iodine chemistry, metal-catalyzed cross-couplings and carbon dioxide fixation. (springer.com)
  • Metal Catalyzed Cross-Coupling Reaction. (whsmith.co.uk)
  • These results revealed a possible cross-reaction between the major linear B-cell epitope of Ro60 protein (216-232aa) and the homologous peptide of Coxsackie virus 3C-protease in SS patients. (biomedcentral.com)
  • Motivated by these unexpected discrepancies, the present study analyses \( \alpha\) -induced reaction cross sections for targets with masses \( A \approx\) 20-50. (springer.com)
  • The (p, tf) reaction on Pu 239 and U 235 targets was used to deduce the σ (Pu 236 (n,f))/σ(U 232 (n,f)) ratio, and the Pu 236 (n,f) cross section was subsequently determined for En=0.5-7.5 MeV. (osti.gov)
  • A newly developed facility at the 3 MV Tandem Accelerator at Dhaka for measurement of proton induced reaction cross sections in the energy region below 5 MeV is outlined and tests for the beam characterization are described. (degruyter.com)
  • Total reaction cross sections are predicted for proton scattering from various nuclei. (edu.au)
  • In this work we studied the proton induced reactions on natural antimony. (elsevier.com)
  • Ko¨ınsidensie data, wat verkry is by tien kwasievrye hoekpare, met proton hoeke tussen 25◦ en 110◦, is geanaliseer in terme van die vervormde-golf-impuls-benadering (DWIA). (sun.ac.za)
  • Carbon-carbon couplings through Suzuki, Sonogashira and Heck reactions are ubiquitous in organic chemistry due to their convenience in producing various chemicals in many industry fields. (scielo.br)
  • Thus, the ability to manipulate the immune system via immunologic cross-reactions should have important potential in both preventive and therapeutic strategies for cancer. (pubmedcentralcanada.ca)
  • Results from performed calculations have been compared with the experimental nuclear reaction data, ENDF/B-VII.b4, JENDL-4.0 and JEFF-3.2 evaluated data. (springer.com)
  • GROUPIE calculates unshielded group averaged cross sections, Bondarenko self-shielded group averaged cross sections, and multiband parameters from data in the ENDF/B format. (oecd-nea.org)
  • LINEAR: Converts cross sections in the ENDF/B format (File 3, 23, and 27) to linearly interpolable form (in energy and cross section) and outputs the result in the ENDF/B format. (oecd-nea.org)
  • RECENT: Reconstructs energy-dependent cross sections from a combination of resonance parameters and tabulated background cross sections in the ENDF/B format. (oecd-nea.org)
  • SIGMA-1: Doppler broadens evaluated cross sections in the linear- linear interpolation form of the ENDF/B format. (oecd-nea.org)
  • The obtained differential cross section is compared to the theoretical results of the Pavia reaction code in which different processes leading to two-nucleon knockout are accounted for microscopically. (surrey.ac.uk)
  • To provide experimental activation cross sections for developing theoretical models and codes allowing calculation of probability of nuclear reactions and data for different practical applications, we perform a systematic study of light ion induced nuclear reactions on various target materials. (elsevier.com)
  • The experimental cross section data of the investigated reactions were compared with values reported in literature and results of theoretical calculations provided by the TALYS code. (elsevier.com)
  • A monoclonal antiidiotype reagent cross-reacted with autoantibodies from six of the seven patients. (rupress.org)
  • We will show that by changing the limiting reagent to the boron compound we can avoid Pd blackout at the end of borylation and achieve faster Suzuki reaction rate and consistent reaction completion times. (aiche.org)
  • Results also supported the contention of emotion-specificity in affective moral reaction: Participants in both India and Britain reported anger in response to autonomy transgressions, but contempt in response to violations of community. (ox.ac.uk)
  • Importantly, these results extend previous research by demonstrating the importance of emotion specificity in moral reactions, as opposed to categorization or dilemma resolution. (ox.ac.uk)
  • Cross-dehydrogenative coupling (CDC), which enables the formation of carbon-carbon (C-C) and C-heteroatom bonds from the direct coupling of two C-H bonds or C-H/X-H bonds, represents a new state of the art in the field of organic chemistry. (springer.com)
  • This review attempts to provide a comprehensive understanding of the evolution of cross-dehydrogenative coupling via iron catalysis, as well as its application in synthetic chemistry. (springer.com)
  • The Cross-Coupling Quick Guide will provide an excellent starting point and solid foothold to demystify palladium cross-coupling chemistry. (sigmaaldrich.com)
  • Home » Questions » Science/Math » Chemistry » Physical chemistry » A chemical reaction takes place in a container of. (transtutors.com)
  • Antisera from humans, horses, dogs, and cattle were tested by indirect fluorescent-antibody assay (IFA) for antibodies reactive with E. equi and other ehrlichiae and tested by immunoblot to identify the specific reactions with E. equi. (asm.org)
  • Some of these cross-reactive antibodies may alter the intestinal barrier integrity, which is the key feature of the early stages of CD and many autoimmune disorders [8, (scirp.org)
  • Cross-reaction was found between S. equina antigens and antibodies in the sera of Wuchereria bancrofti-infected patients, with the highest levels observed between sera of chronic infected patients and Setaria spp. (who.int)
  • A simple reduction scheme using so-called reduced energies \( E_{red}\) and reduced cross sections \( \sigma_{red}\) allows the comparison of heavy-ion-induced reaction cross sections for a broad range of masses of projectile and target and over a wide energy range. (springer.com)
  • A global behavior has been found for strongly bound projectiles whereas much larger reduced cross sections have been observed for weakly bound and halo projectiles. (springer.com)
  • The study shows that the experimental data for \( \alpha\) -induced reactions on nuclei with \( A \approx\) 20-50 deviate slightly from the global behavior of reduced cross sections. (springer.com)
  • Simultaneously with the deviation of reduced cross sections \( \sigma_{red}\) from the general behavior, the outliers 23 Na, 33 S, 36 Ar, and 40 Ar also show significant disagreement between experiment and statistical model calculation. (springer.com)
  • MIXER: Calculates the energy dependent cross sections for a composite mixture. (oecd-nea.org)
  • cross sections, interpolation, reaction rates. (oecd-nea.org)
  • GLUCS is a package of programs for simultaneous evaluation of reaction cross sections. (oecd-nea.org)
  • Evaluations of cross sections and covariances are used as input for incorporat- ing correlated data sets, particularly ratios. (oecd-nea.org)
  • The resulting output evaluations have not only updated cross sections and covariances, but also cross-section covariances. (oecd-nea.org)
  • The cross sections were measured with the activation method using a stacked target irradiation technique. (elsevier.com)
  • The Pu 236 (n,f), Pu 237 (n,f) and Pu 238 (n,f) cross sections have been inferred by utilizing the surrogate ratio method. (osti.gov)
  • Measured and calculated cross sections for interactions of solar radiation with atmospheric species, such as photoabsorption, photoionization, and photodissociation and related processes are surveyed. (wright.edu)
  • Using these descriptors as inputs and reaction yield as output, we show that a random forest algorithm provides significantly improved predictive performance over linear regression analysis. (sciencemag.org)
  • What are the symptoms of an allergic reaction to an insect sting? (ahealthyme.com)
  • Although the cross-sectional design of the study does not allow for conclusions on the direction of effects, findings suggest the importance of motivational factors in the development of specific patterns of depressive symptoms to adverse events. (hindawi.com)
  • The consumption of cross-reactive foods as well as gluten-contaminated foods may be responsible for the continuing symptoms presented by a subgroup of patients with coeliac disease. (scirp.org)
  • Allergic reactions involving the alimentary tract can result in a wide range of symptoms, stretching from harmless tingling of the mouth to life-threatening shock reactions and from lasting a few hours to many days. (foodintolerances.org)
  • Edward Francis (Symptoms, Diagnosis and Pathology of Tularemia, The Journal , Oct. 20, 1928, p. 1155) has called attention to reactions with Brucella abortus in serums from patients with tularemia. (jamanetwork.com)
  • Symptoms of an allergic reaction may worsen over time with repeated exposures to the triggering substance. (livestrong.com)
  • The development of photoredox/Ni dual catalytic methods for cross-coupling has opened new vistas for the construction of carbon-carbon bonds at C(sp3)-hybridized centers. (springer.com)
  • This work accordingly reports results from a 17-country, 6-continent experimental study examining psychophysiological reactions to real video news content. (pnas.org)
  • The study of neutrino reactions will also offer a conclusive test of the hypothesis that the neutrino emitted with the electron is the same as that emitted with the muon. (springer.com)
  • This made it possible to correct the first four experiments against the lexical reaction times collected in the baseline study. (uib.no)
  • Conclusion In this large cross-sectional study, extensive patient reports showed that 'positive' reactions to acupuncture treatment were very common. (bmj.com)
  • This cross-national exploratory study was carried out to assess the nature of traumatic experiences, psychological reactions and challenges related to coping among police officers from Pakistan and the United Kingdom. (hud.ac.uk)
  • In the current study, affective and behavioural reactions to transgressions of autonomy (rights) and community (hierarchy) were examined in India and Britain. (ox.ac.uk)
  • In one computational study, unsaturated organic groups were shown to undergo much easier coupling reaction on the metal center. (wikipedia.org)
  • Results offer the most comprehensive cross-national demonstration of negativity biases to date, but they also serve to highlight considerable individual-level variation in responsiveness to news content. (pnas.org)
  • Results, based on over 1,000 respondents across 17 countries and 6 continents, suggest that there is, on average, a negativity bias in psychophysiological reactions to video news content. (pnas.org)
  • In particular, the results of the reaction nat Ni(p, x) 61 Cu below 3 MeV could serve as beam monitor. (degruyter.com)
  • These results indicate that exposure to RF + UVA causes collagen cross-linking of α1, α2, β1 and β2 chains, producing polymers of approximately 300 kDa and larger. (arvojournals.org)
  • Results At least one short term reaction to acupuncture during or immediately after treatment was reported by 94.6% (CI 94.2 to 95.1) of patients, an average of 1.8 reactions per patient. (bmj.com)
  • Results revealed that although reactions to autonomy transgressions are similar in India and Britain, Indian participants express more moral outrage than do Britons in response to transgressions of community. (ox.ac.uk)
  • The information in this factsheet is to draw your attention to the existence of cross reactions and the fact that they can be the reason for what may initially appear as contradictory results. (wimbledonclinic.co.uk)
  • Stereospecificity is the norm in cross-couplings of stereodefined alkenyl trifluoroborates. (organicreactions.org)
  • For example, in nickel and palladium cross couplings, a zerovalent complex with two vacant sites (or labile ligands) reacts with the carbon halogen bond to form a metal halogen and a metal carbon bond. (wikipedia.org)
  • LINTAB and HEATER Program Package: Computer Codes for Model Parameters and Cross-Section Data (Version 87-1). (oecd-nea.org)
  • Measured data sets, including ratios and absolute and relative cross section data, are read and combined with the input evaluations via the least-squares technique. (oecd-nea.org)
  • The variation of the cross section with energy can only approximately be represented by a Gamow function. (dtic.mil)
  • Determination of the thermal neutron induced O-17(n,alpha) C-14 reaction cross section. (ugent.be)
  • The differential cross section was measured for the 12C(e,e'pp)10Beg.s. reaction at energy and momentum transfers of 163MeV and 198MeV/c, respectively. (surrey.ac.uk)
  • The comparison shows a strong sensitivity to nuclear-structure input and the measured cross section is seen to be dominated by the interplay between long- and short-range nucleon-nucleon correlations. (surrey.ac.uk)
  • Microscopic calculations based on the ab initio self-consistent Green's function method give a reasonable description of the experimental cross section. (surrey.ac.uk)
  • 2016) Reaction Cross Section Of The Two-neutron Halo Nucleus 22C At 235 MeV/nucleon In: The 26th International Nuclear Physics Conference (INPC2016), 11-16 Sep 2016, Adelaide, Australia. (surrey.ac.uk)
  • The reaction cross section (σ R σR ) of the very neutron-rich carbon isotope 22 22 C has been measured on a carbon target at 235 MeV/nucleon. (surrey.ac.uk)
  • Calculated energy-sharing cross section and analyzing power distributions reproduce the data reasonably well. (sun.ac.za)
  • These distributions are very consistent over a wide range of angle pairs at which cross section energy-sharing distributions vary considerably. (sun.ac.za)
  • The effects of spin-orbit distortions were found to be very small near zero recoil momentum and did not destroy the validity of the factorization approximation where the two-body p-α cross section enters as a multiplicative factor in the three-body (p, pα) cross section expression. (sun.ac.za)
  • Although this reaction is catalysed by Ni or Pd complexes, the latter have been much more developed. (rsc.org)