OrganismsDiseasesChemicals and DrugsPhenomena and Processes
CrithidiaCrithidia fasciculataDNA, KinetoplastTrypanosomatinaTrypanosomaEukaryotaAmide SynthasesDNA, CircularRNA, ProtozoanTrypanosoma brucei bruceiRNA, GuideGenes, ProtozoanDNA, ProtozoanTrypanosoma lewisiLeishmaniaEuglenozoa Infections
Crithidia
A genus of parasitic protozoans found in the digestive tract of invertebrates, especially insects. Organisms of this genus have an amastigote and choanomastigote stage in their life cycle.
Crithidia fasciculata
A species of monogenetic, parasitic protozoa usually found in insects.
DNA, Kinetoplast
DNA of kinetoplasts which are specialized MITOCHONDRIA of trypanosomes and related parasitic protozoa within the order KINETOPLASTIDA. Kinetoplast DNA consists of a complex network of numerous catenated rings of two classes; the first being a large number of small DNA duplex rings, called minicircles, approximately 2000 base pairs in length, and the second being several dozen much larger rings, called maxicircles, approximately 37 kb in length.
Trypanosomatina
A suborder of monoflagellate parasitic protozoa that lives in the blood and tissues of man and animals. Representative genera include: Blastocrithidia, Leptomonas, CRITHIDIA, Herpetomonas, LEISHMANIA, Phytomonas, and TRYPANOSOMA. Species of this suborder may exist in two or more morphologic stages formerly named after genera exemplifying these forms - amastigote (LEISHMANIA), choanomastigote (CRITHIDIA), promastigote (Leptomonas), opisthomastigote (Herpetomonas), epimastigote (Blastocrithidia), and trypomastigote (TRYPANOSOMA).
Trypanosoma
A genus of flagellate protozoans found in the blood and lymph of vertebrates and invertebrates, both hosts being required to complete the life cycle.
Eukaryota
One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.
Amide Synthases
Enzymes that catalyze the joining of either ammonia or an amide with another molecule, in which the linkage is in the form of a carbon-nitrogen bond. EC 6.3.1.
DNA, Circular
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
RNA, Protozoan
Ribonucleic acid in protozoa having regulatory and catalytic roles as well as involvement in protein synthesis.
Trypanosoma brucei brucei
A hemoflagellate subspecies of parasitic protozoa that causes nagana in domestic and game animals in Africa. It apparently does not infect humans. It is transmitted by bites of tsetse flies (Glossina).
RNA, Guide
Small kinetoplastid mitochondrial RNA that plays a major role in RNA EDITING. These molecules form perfect hybrids with edited mRNA sequences and possess nucleotide sequences at their 5'-ends that are complementary to the sequences of the mRNA's immediately downstream of the pre-edited regions.
Genes, Protozoan
The functional hereditary units of protozoa.
DNA, Protozoan
Deoxyribonucleic acid that makes up the genetic material of protozoa.
Trypanosoma lewisi
A trypanosome found in the blood of adult rats and transmitted by the rat flea. It is generally non-pathogenic in adult rats but can cause lethal infection in suckling rats.
Leishmania
A genus of flagellate protozoa comprising several species that are pathogenic for humans. Organisms of this genus have an amastigote and a promastigote stage in their life cycles. As a result of enzymatic studies this single genus has been divided into two subgenera: Leishmania leishmania and Leishmania viannia. Species within the Leishmania leishmania subgenus include: L. aethiopica, L. arabica, L. donovani, L. enrietti, L. gerbilli, L. hertigi, L. infantum, L. major, L. mexicana, and L. tropica. The following species are those that compose the Leishmania viannia subgenus: L. braziliensis, L. guyanensis, L. lainsoni, L. naiffi, and L. shawi.
Euglenozoa Infections
Infections with the protozoa of the phylum EUGLENOZOA.

Isolation of tubulin polyglutamylase from Crithidia; binding to microtubules and tubulin, and glutamylation of mammalian brain alpha- and beta-tubulins. (1/119)

Trypanosomatids have a striking cage-like arrangement of submembraneous microtubules. We previously showed that alpha- and beta- tubulins of these stable microtubules are extensively modified by polyglutamylation. Cytoskeletal microtubular preparations obtained by Triton extraction of Leishmania tarentolae and Crithidia fasciculata retain an enzymatic activity that incorporates radioactive glutamic acid in a Mg2+-ATP-dependent manner into alpha- and beta-tubulins. The tubulin polyglutamylase is extracted by 0.25 M salt. The Crithidia enzyme can be purified by ATP-affinity chromatography, glycerol-gradient centrifugation and ion-exchange chromatography. After extraction from the microtubular cytoskeleton the glutamylase forms a complex with alphabeta tubulin, but behaves after removal of tubulin as a globular protein with a molecular mass of 38x10(3). In highly enriched fractions a corresponding band is the major polypeptide visible in SDS-PAGE. The enzyme from Crithidia recognises mammalian brain tubulin, where it incorporates glutamic acid preferentially into the more acidic variants of both alpha- and beta-tubulins. Synthetic peptides with an oligoglutamyl side chain, corresponding to the carboxy-terminal end of brain alpha- and beta-tubulins, are accepted by the enzyme, albeit at low efficiency. The polyglutamylase elongates the side chain by up to 3 and 5 residues, respectively. Other properties of the tubulin polyglutamylase are also discussed.  (+info)

The kinetoplast structure-specific endonuclease I is related to the 5' exo/endonuclease domain of bacterial DNA polymerase I and colocalizes with the kinetoplast topoisomerase II and DNA polymerase beta during replication. (2/119)

The mitochondrial DNA (kinetoplast DNA) of the trypanosomatid Crithidia fasciculata has an unusual structure composed of minicircles and maxicircles topologically interlocked into a single network and organized in a disc-shaped structure at the base of the flagellum. We previously purified a structure-specific endonuclease (SSE1), based on its RNase H activity, that is enriched in isolated kinetoplasts. The endonuclease gene has now been cloned, sequenced, and found to be closely related to the 5' exonuclease domain of bacterial DNA polymerase I proteins. Although the protein does not contain a typical mitochondrial leader sequence, the enzyme is shown to colocalize with a type II DNA topoisomerase and a DNA polymerase beta at antipodal sites flanking the kinetoplast disc. Cell synchronization studies with an epitope-tagged construct show that the localization of the endonuclease to the antipodal sites varies in a cell cycle-dependent manner similar to that of the DNA polymerase beta [Johnson, C. E. & Englund, P. T. (1998) J. Cell Biol. 143, 911-919]. Immunofluorescent localization of SSE1 to the antipodal sites is only observed during kinetoplast replication. Together, these results suggest a point of control for kinetoplast DNA replication through the regulation of the availability of DNA replication proteins and a possible role for the antipodal sites in removal of RNA primers and the repair of gaps in newly replicated minicircles.  (+info)

Molecular characterization of a hyperinducible, surface membrane-anchored, class I nuclease of a trypanosomatid parasite. (3/119)

The 3'-nucleotidase/nuclease (3'-NT/NU) is a surface enzyme unique to trypanosomatid parasites. These organisms lack the pathway for de novo purine biosynthesis and thus are entirely dependent upon their hosts to supply this nutrient for their survival, growth, and multiplication. The 3'-NT/NU is involved in the salvage of preformed purines via the hydrolysis of either 3'-nucleotides or nucleic acids. In Crithidia luciliae, this enzyme is highly inducible. For example, in these organisms purine starvation triggers an approximately 1000-fold up-expression of 3'-NT/NU activity. In the present study, we cloned and characterized a gene encoding this intriguing enzyme from C. luciliae (Cl). Sequence analysis showed that the Cl 3'-NT/NU deduced protein possessed five regions, which we defined here as being characteristic of members of the class I nuclease family. Further, we demonstrated that the Cl 3'-NT/NU-expressed protein possessed both 3'-nucleotidase and nuclease activities. Moreover, we showed that the dramatic up-expression of 3'-NT/NU activity in response to purine starvation of C. luciliae was concomitant with the approximately 100-fold elevation in steady-state mRNA specific for this gene. Finally, results of our nuclear run-on analyses demonstrated that such up-regulation in 3'-NT/NU enzyme activity was mediated at the posttranscriptional level.  (+info)

Atypical processing in domain III of 23S rRNA of Rhizobium leguminosarum ATCC 10004(T) at a position homologous to an rRNA fragmentation site in protozoa. (4/119)

For still unknown reasons, the 23S rRNA of many alpha-Proteobacteria shows a unique fragmentation pattern compared to other bacteria. The 23S rRNA processing involves RNase III and additional, yet unidentified enzymes. The alpha-proteobacterium Rhizobium leguminosarum ATCC 10004(T) possesses two fragmentation sites in its 23S rRNA. The first one harbors an intervening sequence in helix 9 which is cleaved by RNase III. We demonstrate that the mature 5' end of the resulting 2.6-kb rRNA fragment is generated by additional removal of helix 10. A fraction of the 2.6-kb rRNA is further processed in domain III, giving rise to two 1.3-kb rRNA fragments. We mapped the domain III fragmentation site and found it to be at a position which has only been reported for trypanosomatid protozoa. This fragmentation site is also unique in that it lacks an intervening sequence. We found that the simultaneous occurrence of 2.6-kb and 1.3-kb rRNA fragments is not due to interoperonal sequence differences but rather reflects slow processing. The different characteristics of the two fragmentation sites in the 23S rRNA suggest that they are processed by different mechanisms. Interestingly, the amount of 2.6-kb rRNA varies during culture growth. We observed a transient increase in the relative amount of 2.6-kb rRNA fragments during the first hours after inoculation, which points to changes in the ratio of rRNA synthesis rate to domain III processing rate during the growth of a culture.  (+info)

Site-specific interactions of JBP with base and sugar moieties in duplex J-DNA. Evidence for both major and minor groove contacts. (5/119)

Beta-D-Glucosyl-hydroxymethyluracil, also called base J, is an unusually modified DNA base conserved among Kinetoplastida. Base J is found predominantly in repetitive DNA and correlates with epigenetic silencing of telomeric variant surface glycoprotein genes. We have previously identified a J-binding protein (JBP) in Trypanosoma, Leishmania, and Crithidia, and we have shown that it is a structure-specific binding protein. Here we examine the molecular interactions that contribute to recognition of the glycosylated base in synthetic DNA substrates using modification interference, modification protection, DNA footprinting, and photocross-linking techniques. We find that the two primary requirements for J-DNA recognition include contacts at base J and a base immediately 5' of J (J-1). Methylation interference analysis indicates that the requirement of the base at position J-1 is due to a major groove contact independent of the sequence. DNA footprinting of the JBP.J-DNA complex with 1,10-phenanthroline-copper demonstrates that JBP contacts the minor groove at base J. Substitution of the thymine moiety of J with cytosine reduces the affinity for JBP approximately 15-fold. These data indicate that the sole sequence dependence for JBP binding may lie in the thymine moiety of base J and that recognition requires only two specific base contacts, base J and J-1, within both the major and minor groove of the J-DNA duplex.  (+info)

A single enzyme catalyses formation of Trypanothione from glutathione and spermidine in Trypanosoma cruzi. (6/119)

Protozoa of the order Kinetoplastida differ from other organisms in their ability to conjugate glutathione (l-gamma-glutamyl-cysteinyl-glycine) and spermidine to form trypanothione [N(1),N(8)-bis(glutathionyl)spermidine], a metabolite involved in defense against chemical and oxidant stress and other biosynthetic functions. In Crithidia fasciculata, trypanothione is synthesized from GSH and spermidine via the intermediate glutathionylspermidine in two distinct ATP-dependent reactions catalyzed by glutathionylspermidine synthetase (GspS; EC ) and trypanothione synthetase (TryS; EC ), respectively. Here we have cloned a single copy gene (TcTryS) from Trypanosoma cruzi encoding a protein with 61% sequence identity with CfTryS but only 31% with CfGspS. Saccharomyces cerevisiae transformed with TcTryS were able to synthesize glutathionylspermidine and trypanothione, suggesting that this enzyme is able to catalyze both biosynthetic steps, unlike CfTryS. When cultures were supplemented with aminopropylcadaverine, yeast transformants contained glutathionylaminopropylcadaverine and homotrypanothione [N(1),N(9)-bis(glutathionyl)aminopropylcadaverine], metabolites that have been previously identified in T. cruzi, but not in C. fasciculata. Kinetic studies on recombinant TcTryS purified from Escherichia coli revealed that the enzyme displays high-substrate inhibition with glutathione (K(m) and K(i) of 0.57 and 1.2 mm, respectively, and k(cat) of 3.4 s(-1)), but obeys Michaelis-Menten kinetics with spermidine, aminopropylcadaverine, glutathionylspermidine, and MgATP as variable substrate. The recombinant enzyme possesses weak amidase activity and can hydrolyze trypanothione, homotrypanothione, or glutathionylspermidine to glutathione and the corresponding polyamine.  (+info)

Isoenzyme clustering of Trypanosomatidae Colombian populations. (7/119)

Thirty-six Trypanosomatidae stocks isolated from various hosts and geographical areas in Colombia and 7 others from Bolivia, Chile, Honduras and Panama have been surveyed by Multilocus Enzyme Electrophoresis (MLEE). Part of the Colombian stocks were previously characterized by morphology and biological behavior as belonging to Trypanosoma cruzi and Trypanosoma rangeli taxa, others were unknown species. The genetic variability observed at 13 different loci was considerable, since 38 zymodemes could be distinguished and 2 upper branches were observed. The first branch corresponded to T. cruzi and was divided in the two major phylogenetic subdivision of T. cruzi (T. cruzi I, T. cruzi II). The majority of the Colombian T. cruzi stocks (92%) felt into T. cruzi I. Only two stocks, isolated from sylvatic mammals, belonged to T. cruzi II. Among T. cruzi I, we did not observed any additional phylogenetic subdivision and host-dependent genotype specificity. The second branch was genetically very heterogeneous and included all T. rangeli stocks, the stocks isolated from bats and one stock isolated from a sylvatic R. prolixus vector. The stocks belonging to T. rangeli presented only one locus instead of two for the malic enzyme system. Since, the upper level of resolution of the isoenzyme method was exceeded, the current clustering study failed to draw a clear distinction between such a diverse set of Trypanosomatidae species.  (+info)

Genome scan stratified by the presence of anti-double-stranded DNA (dsDNA) autoantibody in pedigrees multiplex for systemic lupus erythematosus (SLE) establishes linkages at 19p13.2 (SLED1) and 18q21.1 (SLED2). (8/119)

Anti-double-stranded DNA (anti-dsDNA) is arguably one of the most specific autoantibodies in systemic lupus erythematosus (SLE). This antibody is associated with more severe SLE and with glomerulonephritis. From 196 pedigrees multiplex for SLE, we selected those that had any SLE affected positive for anti-dsDNA by the Crithidia luciliae kinetoplast imunofluorescence assay. This stratification strategy tested the hypothesis that anti-dsDNA would identify a more genetically homogeneous group of pedigrees, in which previously undetected linkage effects could be established. A genome screen data for linkage to SLE was available at 307 microsatellite markers for this selected group of 71 pedigrees: 37 European-American, 29 African-American, and five others. The most significant results were obtained at 19p13.2 (LOD(max) = 4.93), named SLED1, in the 37 European-American pedigrees using a dominant model with mixed penetrances (92% for females and 49% for males) at 100% homogeneity (theta = 0). A second linkage effect, SLED2, was established in the 29 African-American pedigrees at 18q21.1 (LOD(max) = 3.40) using a recessive model with 100% penetrance (theta = 0.1). Parametric and non-parametric multipoint analyses were performed, which provided further evidence and support of susceptibility genes residing in these regions. In conclusion, two powerful linkages have been detected with SLE based on the presence of anti-dsDNA. These findings show SLE to be a richly complicated disease phenotype that is now ripe for important new discovery through a genetic approach.  (+info)

Tubulin heterogeneity in the trypanosome Crithidia fasciculata...Tubulin heterogeneity in the trypanosome Crithidia fasciculata...
Tubulin heterogeneity in the trypanosome Crithidia fasciculata.: The interphase cell of Crithidia fasciculata has three discrete tubulin populations: the subpel
https://www.mysciencework.com/publication/show/tubulin-heterogeneity-in-the-trypanosome-crithidia-fasciculata
Larvae act as a transient transmission hub for the prevalent bumblebee parasite Crithidia bombi - Research - Royal Holloway,...Larvae act as a transient transmission hub for the prevalent bumblebee parasite Crithidia bombi - Research - Royal Holloway,...
Disease transmission networks are key for understanding parasite epidemiology. Within the social insects, structured contact networks have been suggested to limit the spread of diseases to vulnerable members of their society, such as the queen or brood. However, even these complex social structures do not provide complete protection, as some diseases, which are transmitted by workers during brood care, can still infect the brood. Given the high rate of feeding interactions that occur in a social insect colony, larvae may act as disease transmission hubs. Here we use the bumblebee Bombus terrestris and its parasite Crithidia bombi to determine the role of brood in bumblebee disease transmission networks. Larvae that were artificially inoculated with C. bombi showed no signs of infection seven days after inoculation. However, larvae that received either an artificial inoculation or a contaminated feed from brood-caring workers were able to transmit the parasite to naive workers. These results ...
https://pure.royalholloway.ac.uk/portal/en/publications/larvae-act-as-a-transient-transmission-hub-for-the-prevalent-bumblebee-parasite-crithidia-bombi
Inmunofluorescencia con Crithidia luciliae para la detección de anticuerpos anti-ADN: Imágenes atípicas y su relación con...Inmunofluorescencia con Crithidia luciliae para la detección de anticuerpos anti-ADN: Imágenes atípicas y su relación con...
GRIEMBERG, Gloria et al. Immunofluorescence assay with Crithidia luciliae for the detection of anti-DNA antibodies: Atypical images and their relationship with Chagas disease and leishmaniasis. Medicina (B. Aires) [online]. 2006, vol.66, n.1, pp. 3-8. ISSN 1669-9106.. Anti-native DNA antibodies can be detected by indirect immunofluorescence assay with Crithidia luciliae, displaying an annular image due to a kinetoplast containing double stranded DNA. Other structures such as membrane, flagellum and basal corpuscle can be stained as well, showing what is called atypical fluorescent images. As C. luciliae belongs to the Trypanosomatidae family, which include the human pathogens Trypanosoma cruzi and Leishmania spp., it was considered that these atypical images could be caused by cross-reactions. Serological studies for Chagas disease were performed in 105 serum samples displaying atypical images. Sixty four percent of the samples from non endemic and 78.3% from endemic areas for Chagas disease ...
http://www.scielo.org.ar/scielo.php?script=sci_abstract&pid=S0025-76802006000100002&lng=es&nrm=iso&tlng=en
Crithidia luciliae - WikipediaCrithidia luciliae - Wikipedia
Crithidia luciliae is a flagellate parasitic excavates known to use the housefly Musca domestica as a host.[2] As part of the family of Trypanosomatidae, they are characterised by the presence of a kinetoplast; a complex network of interlocking circular dsDNA molecules. The presence of this kinetoplast means they are important in the diagnosis of systemic lupus erythamatosus (SLE). By using C.luciliae as a substrate for immunofluorescence, the organelle can be used to detect anti-dsDNA antibodies, a common feature of the disease.[3][4]. ...
https://en.wikipedia.org/wiki/Crithidia_luciliae
The Effects of Laser Microbeam Irradiation on the Flagellum of Crithidia (Strigomonas) Oncopelti | Journal of Experimental...The Effects of Laser Microbeam Irradiation on the Flagellum of Crithidia (Strigomonas) Oncopelti | Journal of Experimental...
I. Flagella and cell bodies of Crithidia (Strigomonas) oncopelti were irradiated at preselected points with a pulsed ruby laser microbeam. Results were recorded by high-speed cinephotomicrography. A flagellum could be completely amputated at the irradiated point.. 2. The portion of the flagellum between the cell body and the irradiated point beat from the base after irradiation. The amputated portion of the flagellum could beat from either the tip or the irradiated point, and could beat first from the tip and then from the irradiated point or vice versa. Beating could continue for up to ten cycles.. 3. For flagellar activity in this organism neither a unique region of the flagellum nor the cell body is necessary. Wave propagation appears to involve the transmission of a signal at a greater velocity than that of the wave. The results favour a model of bend propagation which allows for a distribution of autonomous initiators along the flagellar length.. ...
http://jeb.biologists.org/content/53/2/401
Micromanipulation of the Flagellum of Crithidia Oncopelti | Journal of Experimental BiologyMicromanipulation of the Flagellum of Crithidia Oncopelti | Journal of Experimental Biology
When starting her own lab at James Cook University, Australia, Jodie Rummer applied for a Travelling Fellowship from JEB to gather data on oxygen consumption rates of coral reef fishes at the Northern Great Barrier Reef. A few years later, Björn Illing, from the Institute for Hydrobiology and Fisheries Science, Germany, followed in Jodies footsteps and used a JEB Travelling Fellowship to visit Jodies lab. There, he studied the effects of temperature on the survival of larval cinnamon clownfish. Jodie and Björns collaboration was so successful that they have written a collaborative paper, and Björn has now returned to continue his research as a post-doc in Jodies Lab. Read their story here.. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 30 Nov 2017. Apply now!. ...
http://jeb.biologists.org/content/60/2/437
Crithidia - WikipediaCrithidia - Wikipedia
Not to be confused with the fungi genus Chytridium. Crithidia is a genus of trypanosome Euglenozoa. They are parasites that exclusively parasitise arthropods, mainly insects. They pass from host to host as cysts in infective faeces and typically, the parasites develop in the digestive tracts of insects and interact with the intestinal epithelium using their flagellum. They display very low host-specificity and a single parasite can infect a large range of invertebrate hosts. At different points in its life-cycle, it passes through amastigote, promastigote, and epimastigote phases; the last is particularly characteristic, and similar stages in other trypanosomes are often called crithidial. Crithidia bombi is perhaps the most well documented species and is the most prevalent parasite of bumblebees, including common species like Bombus terrestris, Bombus muscorum, and Bombus hortorum. The parasites negatively impact reproductive fitness of Bombus queens, as they affect their ovarian development as ...
https://en.wikipedia.org/wiki/Crithidia
Preparation of Mitochondria from Protozoa and Algae<...Preparation of Mitochondria from Protozoa and Algae<...
TY - JOUR. T1 - Preparation of Mitochondria from Protozoa and Algae. AU - Buetow, D. E.. PY - 1970/1/1. Y1 - 1970/1/1. N2 - This chapter discusses the preparation of mitochondria from protozoa and algae. The isolation and properties of mitochondria from various higher plants and from the tissues of animals are extensively studied. Oxidative phosphorylation has been observed in cell-free systems isolated from bacteria, yeast, and Neurospora. Until recently there was little success in the isolation of mitochondria from protozoa and algae. This chapter brings together the recently developed techniques on these latter cell types. So far, tightly coupled mitochondria have been isolated only from Tetrahymena pyriformis and from Prototheca zopfii. A wide variety of techniques for determining yields of mitochondria1 protein are available. Two commonly used techniques are those of Lowry et al. and Gornall et al. The trypanosome Crithidia fasciculata has been used recently in mitochondria studies by ...
https://experts.illinois.edu/en/publications/preparation-of-mitochondria-from-protozoa-and-algae
Pollen extracts increase growth of a trypanosome parasite of bumble bees [PeerJ Preprints]Pollen extracts increase growth of a trypanosome parasite of bumble bees [PeerJ Preprints]
Phytochemicals produced by plants, including at flowers, function in protection against plant diseases, and have a long history of use against trypanosome infection. Floral nectar and pollen, the sole food sources for many species of insect pollinators, contain phytochemicals that have been shown to reduce trypanosome infection in bumble and honey bees when fed as isolated compounds. Nectar and pollen, however, consist of phytochemical mixtures, which can have greater antimicrobial activity than do single compounds. This study tested the hypothesis that pollen extracts would inhibit parasite growth.Extracts of six different pollens were tested for direct inhibitory activity against cell cultures of the bumble bee trypanosome gut parasite Crithidia bombi. Surprisingly, pollen extracts increased parasite growth rather than inhibiting it. Experimental manipulations of growth media showed that supplemental monosaccharides (glucose and fructose) were sufficient to promote growth, while a common floral
https://peerj.com/preprints/2730/
Crithidia confusa Maslov and Lukes ATCC ® PRA-346™Crithidia confusa Maslov and Lukes ATCC ® PRA-346™
Crithidia confusa ATCC ® PRA-346™ Designation: 320AR Isolation: Intestine of Largus cf. cinctus (Heteroptera), Alajuela Province, Costa Rica, 2009
https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Protozoa_Alphanumeric/PRA-346.aspx
Orgentec « ЕЛТА 90Orgentec « ЕЛТА 90
Anti-Nuclear Antibodies HEp-2 (ANA-HEp-2), HEp-2 Cells; Anti-nDNA Antibodies (nDNA), Crithidia Luciliae; Anti-Endomysium Antibodies (AEA), Monkey Esophagus; Anti-Thyroid Antibodies (ATA), Monkey Thyroid; Autoantibodies RL/RK/RS, Rat Liver/Kidney/Stomach; Autoantibodies MsL/MsK/MsS Mouse Liver/Kidney/Stomach; Autoantibodies RL/RKm/RS Rat Liver / Kidney (with medulla/Stomach); Anti-Smooth Muscle Antibodies (ASMA) RatStomach; Anti-Gliadin Antibodies (AGA) Rat Kidney; Anti-Mitochondrial Antibodies (AMA) Rat Kidney; Autoantibodies RK/RS Rat Kidney / Stomach; Autoantibodies MsK/MsS Mouse Kidney / Stomach; Anti-Nuclear Antibodies RL (ANA-RL) Rat Liver. Cells Slides Boxes: ANA-HEp-2 Anti-nDNA Antibodies (nDNA ...
http://www.elta90.com/%D0%BF%D1%80%D0%BE%D0%B4%D1%83%D0%BA%D1%82%D0%B8/%D0%B4%D0%B8%D0%B0%D0%B3%D0%BD%D0%BE%D1%81%D1%82%D0%B8%D1%87%D0%BD%D0%B8-%D0%BA%D0%B8%D1%82%D0%BE%D0%B2%D0%B5/orgentec/
Agashya: Dore ikintu gikomeye utaruzi ko Cristiano Ronaldo na Messi bahuriyeho - YEGOBAgashya: Dore ikintu gikomeye utaruzi ko Cristiano Ronaldo na Messi bahuriyeho - YEGOB
Mu nkuru zacu zashize arizo:Biratangaje: Umunsi w’amateka mu buzima bwa Cristiano Ronaldo wageze, nyuma yo gusezererwa mu irushanwa rya Confederation, ndetse Amakuru mashya : Cristiano Ronaldo yibarutse abana babiri b’impanga (isomere) twabagejejeho ko uyu mugabo Cristiano Ronaldo aherutse kwibaruka impanga zabana babiri, gusa akaba agiye nawe kubabona ku nshuro ye yambere nyuma yo kuva mu marushanwa ya Confederation, igitangaje nuko umwana umwe muri izi mpanga yahisemo kumwita izina rya Mateo Ronaldo. Igitangaje muri ibi nuko na Lionel Messi afite umwana umwe muri babiri afite witwa Mateo Messi, aba bagabo bombi bafatwa nkabahangana cyane ku isi bakaba bafite abana bitiranwa, gusa amakuru dukesha inshuti za hafi za Ronaldo nuko iri zina rya Mateo yarivanye ku mwana wa Lionel Messi mu rwego rwo kwereka isi ko nta bukeba buri hagati yabo bombi ndetse ko ari ninshuti mu buzima busanzwe.. ...
http://yegob.rw/agashya-dore-ikintu-gikomeye-utaruzi-ko-cristiano-ronaldo-na-messi-bahuriyeho/
Pumpa CO-TWO Syncros HV Mini - SyncrosPumpa CO-TWO Syncros HV Mini - Syncros
CO-TWO je praktické kombo CO2 hustilky a klasické mini. Hliníkové tělo v kombinaci s CO2 bombičkou nahustí cyklopneumatiku téměř okamžitě. Dodávaná s duální koncovkou pro galuskové i auto ventilky.
http://www.syncros.com/cz/cs/products/238607/pumpa-co-two-syncros-hv-mini
umukunzi - Tubeho Neza
Kugira ngo inyungu yumvikane neza, turavuga ku bashakanye bombi umugabo numugore. Niba umubano wanyu wari usanzwe umeze neza, ibihe bya « Guma mu rugo » ni ibyo kurushaho kuwenyegeza. Niba wacumbagiraga, ni umwanya wo kuwubyutsa mukawubungabunga ugasubira kuba mwiza. Ibi mbasabye kubyitondera cyane kuko hari abo « Guma mu rugo » yagizeho ingaruka mbi, nko mu Bushinwa icyorezo cyageze …. « Guma mu rugo »; uko abashakanye babyungukiramo Read More ». ...
https://tubehoneza.com/tag/umukunzi/
Nosema bombi (Microsporidia: Nosematidae) and trypanosomatid prevalence in spring bumble bee queens (Hymenoptera: Apidae:...Nosema bombi (Microsporidia: Nosematidae) and trypanosomatid prevalence in spring bumble bee queens (Hymenoptera: Apidae:...
Several species of bumble bees are declining in the United States; these declining populations often show higher prevalence of Nosema bombi, a microsporidian pathogen. To date, surveys of bumble bee pathogens in the United States have only been conducted on workers and males, yet the health of a population is ultimately dependent on the success of colony-founding queens. We conducted a molecular-diagnostic survey of the prevalence of N. bombi and trypanosomatids, such as Crithidia bombi, in six species of spring queens (n = 142) collected in 2011 and 2013 at three sites in central Kansas. Nosema bombi was found in 27% of Bombus pensylvanicus and 13% of B. auricomus but was not found in the other species sampled. Trypanosomatids were only found in B. pensylvanicus (9%) during the May 2013 sampling period. The high prevalence of N. bombi in B. pensylvanicus is consistent with other surveys for this pathogen in other castes, but the high prevalence of N. bombi in B. auricomus is a novel finding. ...
http://krex.k-state.edu/dspace/handle/2097/18657
Large-scale cultivation of the bumblebee gut microbiota reveals an underestimated bacterial species diversity capable of...Large-scale cultivation of the bumblebee gut microbiota reveals an underestimated bacterial species diversity capable of...
A total of 1940 isolates from gut samples of 60 bumblebees representing Bombus pascuorum, Bombus terrestris, Bombus lucorum and Bombus lapidarius was collected and identified through state-of the-art taxonomic methods. The bacterial species diversity in these Bombus species exceeded that suggested by phylotype analysis through 16S rRNA amplicon sequencing, and revealed that B. pascuorum and B. terrestris had a unique microbiota composition, each. Representatives of most phylotypes reported earlier and detected in the present study were effectively isolated, and included several novel bacterial taxa and species reported for the first time in the bumblebee gut. Isolates were screened in pectin degradation assays and growth inhibition assays against the honeybee pathogens Paenibacillus larvae, Melissococcus plutonius and Ascosphaera apis and the bumblebee parasite Crithidia bombi. While inhibitory activity against each of these pathogens was observed, only one single culture was able to degrade ...
https://jgi.doe.gov/publication/large-scale-cultivation-of-the-bumblebee-gut-microbiota-reveals-an-underestimated-bacterial-species-diversity-capable-of-pathogen-inhibition/
SAT0662 Evaluation of anti-double stranded dna antibodies in the monitoring of systemic lupus erythematosus | Annals of the...SAT0662 Evaluation of anti-double stranded dna antibodies in the monitoring of systemic lupus erythematosus | Annals of the...
Methods A cohort of 36 subjects with active SLE presenting with classical complement activation were enrolled and followed monthly for 1 year. At each study visit blood was collected, serum isolated and frozen until analysis. A total of 371 specimens were collected. Disease activity was scored on the day of each study visit according to the SELENA-SLEDAI method excluding anti-dsDNA or complement components (non-serological [ns] SELENA-SLEDAI). All specimens were tested using four different anti-dsDNA kits; QUANTA Lite, QUANTA Flash, a high Avidity anti-dsDNA ELISA, and the Crithidia luciliae indirect immunofluorescence assay (CLIFT) (Inova Diagnostics, San Diego, CA). Study visits presenting with inactive disease (ns-SELENA- SLEDAI score=0) were compared to those presenting with active disease (ns-SELENA- SLEDAI,0). The longitudinal data were analyzed using linear mixed effect modeling with the ns-SELENA-SLEDAI as dependent variable and the anti-dsDNA titers as fixed effect predictors. Marginal ...
http://ard.bmj.com/content/76/Suppl_2/1025.1
The ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a functionThe ubiquitous gp63-like metalloprotease from lower trypanosomatids: in the search for a function
In addition to these heteroxenous organisms, several genera such as Crithidia, Blastocrithidia, Leptomonas, Herpetomonas and Rhynchoidomonas are restricted to a single host from such widely diverse groups of invertebrates as ciliates, rotifers, nematodes, mollusks, annelids and arachnids, but are found predominantly in insects (Wallace 1966, McGhee and Cosgrove 1980, Vickerman 1994). Insect trypanosomatids have been traditionally allocated to distinct genera that were described based on morphological features, host and geographical origin (Wallace et al. 1983, Momen 2001). However, for identification purposes, these criteria proved to be impractical and insufficient, because different genera share the same evolutive forms (Wallace 1966) and because a diverse range of insects and plants can alberg lower trypanosomatids (Catarino et al. 2001). Therefore, the same trypanosomatid species may be recovered from diverse species of insects or plants and the same insect species may harbor various species ...
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400006&lng=en&tlng=en
Methods » Sivert, Dolok, Charles and Taklar Hong kongMethods » Sivert, Dolok, Charles and Taklar Hong kong
The most parsimonious tree was obtained via using DHFR-TS sequences of Crithidia fasciculata, a monogenetic trypanosomatid, as outgroup (Fernandes and Beverley
http://trainer-trainer.com/t240f14-Sivert-Dolok-Charles-and-Taklar-Hong-kong.html
Qualitatively different immune response of the bumblebee host, Bombus terrestris, to infection by different genotypes of the...Qualitatively different immune response of the bumblebee host, Bombus terrestris, to infection by different genotypes of the...
Qualitatively different immune response of the bumblebee host, Bombus terrestris, to infection by different genotypes of the trypanosome gut parasite, Crithidia ...
https://www.research-collection.ethz.ch/handle/20.500.11850/72286
RCSB PDB for 1OC8RCSB PDB for 1OC8
1OC8: Tryparedoxins from Crithidia Fasciculata and Trypanosoma Brucei: Photoreduction of the Redox Disulfide Using Synchrotron Radiation and Evidence for a Conformational Switch Implicated in Function
http://www.rcsb.org/pdb/explore/biologyAndChemistry.do?structureId=1OC8
10:13 am10:13 am
The deformed wing virus (DWV), known to be ubiquitous in honey bees, has now been detected in bumblebees. In addition, the neogregarine Apicystis bombi has been discovered to be more prevalent than previously thought. Here, we assess for the first time the lethal and sublethal effects of these parasites during single and mixed infections of worker bumblebees (Bombus terrestris). Overall, we find that A. bombi exhibits both lethal and sublethal effects. DWV causes lethal effect and may reduce the sub lethal effects imposed by A. bombi. The results show that both parasites have significant, negative effects on bumblebee health, making them potentially of conservation concern. more here: http://graystock.info/publications/single-and-mixed-infections-of-apicystis-bombi-and-deformed-wing-virus-2015/. ...
http://www.graystock.info/deadly-effects-of-emerging-disease-apicystis-bombi-and-deformed-wing-virus-in-bumblebees/
Poop Proof: Ancient Greeks Suffered from Gut Parasites | Live SciencePoop Proof: Ancient Greeks Suffered from Gut Parasites | Live Science
Researchers recently unearthed the first archaeological evidence of intestinal parasites described by Hippocrates 2,500 years ago.
https://www.livescience.com/61198-hippocrates-poop-parasites-proof.html
Excavata Page 4Excavata Page 4
ATCC holdings include genera such as Crithidia, Leishmania, Trypanosoma, Leptomonas, Euglena, Naegleria, Trichomonas, and Giardia.
https://www.atcc.org/en/Products/Cells_and_Microorganisms/Protozoa/Living_Stock_Protists/Excavata.aspx?dsNav=Ro:10,Up:Page_Product_Listing
Description of the Pictorial Assessment of Interpersonal Relationship (PAIR) in: Magyar Pszichológiai Szemle Volume 67 Issue 2 ...Description of the Pictorial Assessment of Interpersonal Relationship (PAIR) in: Magyar Pszichológiai Szemle Volume 67 Issue 2 ...
A tanulmány a Bombi és munkatársai (Bombi, Pinto és Cannoni, 2007) által kifejlesztett PAIR (Pictorial Assessment of Interpersonal Relationship) rajzelemző eljárást ismerteti, mely módszer segítségével a 6-14 éves gyermekek interperszonális kapcsolatokat ábrázoló rajzai elemezhetők és hasonlíthatók össze hat fő szempont mentén (kohézió, távolítás, hasonlóság, érték, érzelmek és konfliktus). Az eljárás olyan kommunikációs eszközként kezeli a rajzokat, mely kiválóan alkalmas a gyermekek szociális világról, társas jelenségekről és társas érzelmekről való tudása tudományosan megbízható megismerésére. Segítségével feltárható hogyan értelmezik a gyermekek az interperszonális jelenségeket (például barátság, versengés, együttműködés stb.), a társadalmi státuszt (szegény, gazdag), milyen módon differenciálnak a különböző társas kapcsolatok között (például baráti és testvérkapcsolat, győztes és vesztes), és ...
https://akjournals.com/abstract/journals/0016/67/2/article-p267.xml
www.military-paintball.cz :: Zobrazit t ma - Pistole T68www.military-paintball.cz :: Zobrazit t ma - Pistole T68
Trochu bych to poopravil, zku enost s Tiberiem m m, mysl m v ce ne dost. Kule pou v m z sadn Frostbite, kter maj velkou odolnost v i deformac m a po as (dvou a t denn akci v pohod vydr ). P esnost jsou sice hor oproti Titanium premium, kter pou v m v hlavn zbrani, ale netrhnou se a d se s nimi i m it a zas hnout, co je u prachovek s zka do loterie. Pl tv n s bombi kami se vyh b m t m, e v z lo n m z sobn ku bombi ku pouze z lehka dot hnu a propichuji t sn p ed zasunut m do zbran (u z sobn k nov ho typu, se star m proveden m je to t kop dn ). ...
http://forum.military-paintball.cz/viewtopic.php?p=120347
Comprehensive Digestive Stool Analysis - DoctorMyhillComprehensive Digestive Stool Analysis - DoctorMyhill
The test gives an idea of how well foods are digested and absorbed, gives some products of gut fermentation, looks for blood in the stool, gives counts of bacteria and yeast, identifies those bacteria which should not be there and gives a list of antibiotic and anti-fungal preparations, both herbal and drug, to which that micro-organism is sensitive. N.B. If this test is requested as a second or subsequent test on an order, please contact the Office because there will be a small additional interpretation fee due to the complexity of this test result. Please see Ordering Tests for worked examples of how to calculate the costs of tests and their interpretations. The stool analysis is carried out at Genova Diagnostics in the US. Please, note this test does not include looking for gut parasites. For that, see Comprehensive Digestive Stool Analysis with parasitology or Parasitology. A second test, CDSA 2.0 was then designed with some markers that supersede the original CDSA. CDSA 2.0 does not report ...
http://drmyhill.co.uk/wiki/Comprehensive_Digestive_Stool_Analysis
People - Sanford School of Public PolicyPeople - Sanford School of Public Policy
Putnick, DL; Bornstein, MH; Lansford, JE; Malone, PS; Pastorelli, C; Skinner, AT; Sorbring, E; Tapanya, S; Uribe Tirado, LM; Zelli, A; Alampay, LP; Al-Hassan, SM; Bacchini, D; Bombi, AS; Chang, L; Deater-Deckard, K; Di Giunta, L; Dodge, KA; Oburu, P. Perceived mother and father acceptance-rejection predict four unique aspects of child adjustment across nine countries.. The Journal of Child Psychology and Psychiatry and Allied Disciplines 56.8 (August, 2015): 923-932. [doi] [abs] ...
http://fds.duke.edu/db/Sanford/faculty/dodge/publications
THE LOSS OF KINETOPLASTIC DNA IN TWO SPECIES OF TRYPANOSOMATIDAE TREATED WITH ACRIFLAVINE | JCBTHE LOSS OF KINETOPLASTIC DNA IN TWO SPECIES OF TRYPANOSOMATIDAE TREATED WITH ACRIFLAVINE | JCB
The effects of acriflavine on two species of Trypanosomatidae, Crithidia luciliae and Trypanosoma mega, have been investigated. It has been observed that kinetoplastic (i.e. mitochondrial) DNA is lost in a high percentage of acriflavine-treated cells. Resting flagellates, from stationary-phase or hemin-deficient cultures, are considerably more resistant to the acridine than are flagellates from a log-phase culture. When the kinetoplast has retained some DNA and still remains visible in stained smears, it appears reduced in size, and its ultrastructure is extremely abnormal: the DNA fibrils, clearly visible in normal kinetoplasts, are condensed; they appear as an electron-opaque, apparently homogeneous mass, separated from the membranes by a space of low electron-opacity. Analyses of DNA extracts, with high speed centrifugation in CsCl density gradients, revealed that the satellite band, presumably kinetoplastic DNA, is lost by trypanosomes grown for 5 days in the presence of acriflavine. ...
http://jcb.rupress.org/content/34/2/489
Diagnostic accuracy of currently available anti-double-stranded DNA antibody assays. An Italian multicentre study | IRIS...
Objective: An Italian multicentre study was promoted in order to assess the accuracy of four anti-double-stranded DNA (dsDNA) antibody assays for SLE diagnosis and monitoring. Methods: Two hundred and twenty-three patients with established SLE according to ACR classification criteria were enrolled from 9 centres. They included 59 patients at first evaluation (disease duration ,12 months) and 164 with longer disease duration (median disease duration 120 months). The sera from 55 healthy subjects and 161 patients with rheumatic, infectious or neoplastic diseases were tested as controls. SLE activity was measured by ECLAM score. Anti-dsDNA antibodies were detected in serum by means of FarrzymeTM assay, fluoroenzymeimmunoassay (EliATM), Crithidia luciliae indirect immunofluorescence (CLIFT) or Farr radioimmunoassay (Farr). Cut-off values of quantitative assays were chosen by ROC curves analysis. Statistics were conducted by SPSS software package. Results: Sensitivity for SLE diagnosis ranged between ...
https://iris.uniroma1.it/handle/11573/380380
Bumble Bee Disease & Reproduction Is Shaped by Flowering Strip PlantsBumble Bee Disease & Reproduction Is Shaped by Flowering Strip Plants
Flowering strips - pollinator-friendly rows of plants that increase foraging habitat for bees - can help offset pollinator decline but may also bring risks of higher pathogen infection rates for pollinators foraging in those strips.. Researchers from North Carolina State University and the University of Massachusetts Amherst show that flowering strip plants generally benefitted bee colony reproduction by adding floral resources for common eastern bumble bees (Bombus impatiens). The results came with a tradeoff, however, as bumble bees exposed to certain plants showed higher rates of infection by a bee pathogen acquired on flowers - Crithidia bombi, which is associated with reduced bee-foraging abilities as well as mortality in food-compromised bees.. The study showed that certain high-infection plants doubled C. bombi infection intensity when compared with low-infection plant species. Bees feeding mostly on canola plants - a major bee foraging plant and important U.S. crop - showed infection ...
https://scitechdaily.com/bumble-bee-disease-reproduction-is-shaped-by-flowering-strip-plants/
Proteasomal degradation of a trypanosomal ornithine decarboxylaseProteasomal degradation of a trypanosomal ornithine decarboxylase
article{9d3be0e5-e41f-4a44-8c51-3977de05b2b1, abstract = {Mammalian ornithine decarboxylase (ODC), which catalyses the first step in polyamine biosynthesis, has a very fast turnover. It is degraded by the 26S proteasome in an ubiquitin-independent process and the degradation is stimulated by polyamines in a feedback control of the enzyme. Interestingly, there is a major difference in the metabolic stability between ODCs from various trypanosomatids. Trypanosoma brucei and Leishmania donovani both contain stable ODCs, whereas Crithidia fasciculata has an ODC with a rapid turnover. In spite of the difference in stability there is a high degree of sequence homology between C. fasciculata ODC and L. donovani ODC. In the present study we demonstrate that C. fasciculata ODC is rapidly degraded also in mammalian systems like CHO cells and rabbit reticulocyte lysate, suggesting that the degradation signals of the enzyme are recognised by the mammalian systems. L. donovani ODC, on the other hand, is ...
https://lup.lub.lu.se/search/publication/296553
Efficacy of common laboratory disinfectants and heat on killing trypanosomatid parasites | Parasites & Vectors | Full TextEfficacy of common laboratory disinfectants and heat on killing trypanosomatid parasites | Parasites & Vectors | Full Text
The disinfectants TriGene, bleach, ethanol and liquid hand soap, and water and temperature were tested for their ability to kill bloodstream forms of Trypanosoma brucei, epimastigotes of Trypanosoma rangeli and promastigotes of Leishmania major. A 5-min exposure to 0.2% TriGene, 0.1% liquid hand soap and 0.05% bleach (0.05% NaOCl) killed all three trypanosomatids. Ethanol and water destroyed the parasites within 5 min at concentrations of 15-17.5% and 80-90%, respectively. All three organisms were also killed when treated for 5 min at 50°C. The results indicate that the disinfectants, water and temperature treatment (i.e. autoclaving) are suitable laboratory hygiene measures against trypanosomatid parasites.
https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-1-35
Kaelynn Parker - UMW Undergraduate Research and CreativityKaelynn Parker - UMW Undergraduate Research and Creativity
By Kaelynn Parker and Abigail Delapenha. Faculty mentor: Professor Swati Agrawal. Crithidia fasciculata belongs to a group of parasites called kinetoplastids that comprise many important human pathogens. Evidence of apoptosis has been found in these parasites with pathways that appear to be different than in mammalian cells. Therefore, careful characterization of these pathways can provide ways to manipulate parasite infection which could be used to create better treatments for these diseases. In this study, potential apoptosis genes conserved across all kinetoplastid parasites were identified using gene prediction programs in Tri-TrypDB and BLAST searches. Homologous genes were identified in C. fasciculata and a comprehensive q-PCR analysis showed differential upregulation upon induction of apoptosis. One of the genes significantly changed was Bax1 inhibitory gene (Bax1i), an inhibitor of the putative apoptosis promoting Bax1. In order to characterize this gene further we made gene modification ...
https://2021.umwrcd.net/tag/kaelynn-parker/
Abigail Delapenha - UMW Undergraduate Research and CreativityAbigail Delapenha - UMW Undergraduate Research and Creativity
By Kaelynn Parker and Abigail Delapenha. Faculty mentor: Professor Swati Agrawal. Crithidia fasciculata belongs to a group of parasites called kinetoplastids that comprise many important human pathogens. Evidence of apoptosis has been found in these parasites with pathways that appear to be different than in mammalian cells. Therefore, careful characterization of these pathways can provide ways to manipulate parasite infection which could be used to create better treatments for these diseases. In this study, potential apoptosis genes conserved across all kinetoplastid parasites were identified using gene prediction programs in Tri-TrypDB and BLAST searches. Homologous genes were identified in C. fasciculata and a comprehensive q-PCR analysis showed differential upregulation upon induction of apoptosis. One of the genes significantly changed was Bax1 inhibitory gene (Bax1i), an inhibitor of the putative apoptosis promoting Bax1. In order to characterize this gene further we made gene modification ...
https://2021.umwrcd.net/tag/abigail-delapenha/
Prediction of inhibitor binding free energies by quantum neural networks. Nucleoside analogues binding to trypanosomal...Prediction of inhibitor binding free energies by quantum neural networks. Nucleoside analogues binding to trypanosomal...
TY - JOUR. T1 - Prediction of inhibitor binding free energies by quantum neural networks. Nucleoside analogues binding to trypanosomal nucleoside hydrolase. AU - Braunheim, Benjamin B.. AU - Miles, Robert W.. AU - Schramm, Vern L.. AU - Schwartz, Steven D.. PY - 1999/12/7. Y1 - 1999/12/7. N2 - A computational method has been developed to predict inhibitor binding energy for untested inhibitor molecules. A neural network is trained from the electrostatic potential surfaces of known inhibitors and their binding energies. The algorithm is then able to predict, with high accuracy, the binding energy of unknown inhibitors. IU-nucleoside hydrolase from Crithidia fasciculata and the inhibitor molecules described previously [Miles, R. W. Tyler, P. C. Evans, G. Fumeaux R. H., ParK(i)n, D. W., and Schramm, V. L. (1999) Biochemistry 38, xxxx-xxxx] are used as the test system. Discrete points on the molecular electrostatic potential surface of inhibitor molecules are input to neural networks to identify the ...
https://einstein.pure.elsevier.com/en/publications/prediction-of-inhibitor-binding-free-energies-by-quantum-neural-n-2
Project : USDA ARSProject : USDA ARS
Objective 1: Improve the production and management of non-Apis bees such as blue orchard bees, bumble bees, and alfalfa leafcutting bees for crop pollination by increasing knowledge of bee nutritional needs and environmental effects on bee physiology (especially on diapause and overwintering). Sub-Objective 1.1: Identify the pollen and nectar requirements for maintaining non-Apis bee fitness, in both native and managed ecosystems. Sub-Objective 1.2: Develop a better understanding of the environmental factors that affect diapause in non-Apis bees, and develop methods to improve winter survival. Objective 2: Identify environmental (e.g. poor nutrition) and biological factors associated with bee declines (non-Apis species and the honey bee) and develop methods to diagnose and control non-Apis mortality, such as pollen ball and chalkbrood, that are caused by parasites, pathogens (e.g. Crithidia and viruses of bumble bees), and pesticides. Sub-Objective 2.1: For non-Apis bees, develop methods to ...
https://www.ars.usda.gov/research/project/?accnNo=425663
Slow Growth Rate for Fahaka - Page 2 - The Puffer ForumSlow Growth Rate for Fahaka - Page 2 - The Puffer Forum
Gut parasites tend to shed eggs/infectious sections constantly when mature. Other fish definitely investigate the waste o other fish, as few fish have appetite suppression when they are full - when food is abundant (standard in captivity if suitable foods are used), fish will continue eating so long as food is available, even if undigested food is being pushed out the other end. Investigating other fishs waste is actually and effective feeding practice in the wild. There it lack the density we have in captive systems. But it does increase to chance of parasite infestation. In the wild, if that gives the fish the extra nutrition need to reproduce sooner or lager numbers of eggs/sperm, they may increase theor numbers in the next generation even if they may die sooner from parasites. Survival goals in captivity are quite different drom those in the wild ...
https://www.thepufferforum.com/forum/viewtopic.php?p=329660
Amphidinium | Semantic ScholarAmphidinium | Semantic Scholar
Abstract. We show using PCR that psbC, atpA and petB genes are present in the plastid DNA minicircles from the dinoflagellate… Expand ...
https://www.semanticscholar.org/topic/Amphidinium/525126
Center for Child & Family PolicyCenter for Child & Family Policy
Lansford, JE; Woodlief, D; Malone, PS; Oburu, P; Pastorelli, C; Skinner, AT; Sorbring, E; Tapanya, S; Tirado, LMU; Zelli, A; Al-Hassan, SM; Alampay, LP; Bacchini, D; Bombi, AS; Bornstein, MH; Chang, L; Deater-Deckard, K; Di Giunta, L; Dodge, KA, A longitudinal examination of mothers and fathers social information processing biases and harsh discipline in nine countries., Development and Psychopathology, vol. 26 no. 3 (August, 2014), pp. 561-573, ISSN 0954-5794 [doi] [abs] [parenting ...
https://fds.duke.edu/db?pubs-67--1666---9-1958
Glycerol - WikipédiaGlycerol - Wikipédia
Glycerol sa používa o. i. v kozmetických výrobkoch, hlavne ako prísada v hydratačných krémoch a mydlách, ako prísada do zubných pást, pri výrobe plastických hmôt hlavne ako zmäkčovadlo, liečiv, žuvačiek, pást, farbív a výbušnín. Používa sa tiež pri výrobe bezvodého etanolu pre odstránenie prímesi vody. Je súčasťou nemrznúcich zmesí, väčšinou v kombinácii s etylénglykolom. V potravinárskom priemysle sa užíva ako prísada pre úpravu nápojov a mene kvalitných vín, ako sladidlo napr. v šľahačkách v bombičkách, ako zmäkčovadlo želé a iných cukroviniek a pod. Ako súčasť potravín má označenie E 422. V lekárstve sa užíva pri liečbe opuchov mozgu ako prísada infúzií, na znižovanie vnútroočného tlaku, pri zápchach, na zmäkčenie stolice vo forme glycerínových čípkov alebo ako súčasť klystíru. ...
https://sk.m.wikipedia.org/wiki/Glycer%C3%ADn
Continental Class installations: Discontinuity drive - Everything2.comContinental Class installations: Discontinuity drive - Everything2.com
This node is fictional. Refer to Continental Class Space Battleships. The discontinuity drive a/k/a D generator is what propels a Continenta...
https://everything2.com/title/Continental+Class+installations%253A+Discontinuity+drive
Moho Discontinuity - Explanation, Discovery, Role and FAQsMoho Discontinuity - Explanation, Discovery, Role and FAQs
Learn about moho discontinuity topic of Geography in details explained by subject experts on vedantu.com. Register free for online tutoring session to clear your doubts.
https://www.vedantu.com/geography/moho-discontinuity
MYSTERIES OF THE TRYPANOSOMATID MAXICIRCLES: CHARACTERIZATION OF THE M by Preethi Ranganathan IyengarMYSTERIES OF THE TRYPANOSOMATID MAXICIRCLES: CHARACTERIZATION OF THE M by Preethi Ranganathan Iyengar
The trypanosomatid protists belonging to Order Kinetoplastida are some of the most successful parasites ever known to mankind. Their extreme physiological diversity and adaptability to different environmental conditions and host systems make them some of the most widespread parasites, causing deadly diseases in humans and other vertebrates. This project focuses on their unique mitochondrion, called the kinetoplast, and more specifically involves the characterization of a part of their mitochondrial DNA (also called kinetoplast DNA or kDNA), the maxicircles, which are functional homologs of eukaryotic mitochondrial DNA in the kinetoplastid protists. We have sequenced and characterized the maxicircle genomes of 20 new trypanosomatids and compared them with 8 previously published maxicircle genomes of other trypanosomatids. Transcripts of ~13 of the 20 total genes in these maxicircles undergo post-transcriptional modifications involving the insertion and deletion of U residues at precise sites, to yield
https://scholarscompass.vcu.edu/etd/4010/
MC-Easy™ Minicircle DNA Production Kit (with Competent Cells) | System BiosciencesMC-Easy™ Minicircle DNA Production Kit (with Competent Cells) | System Biosciences
When you want sustained transgene expression without introducing any foreign DNA-such as for model animal and gene therapy development-Minicircle Technology is a great gene expression option. Produced as small excised, circular DNA fragments from a parental plasmid, the non-viral, episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than whats possible with standard-sized plasmids, and, while Minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.. Product Note:. Parental minicircle plasmids and the ZYCY10P3S2T Producer Bacterial Strain are available for purchase by not-for-profit researchers only. Commercial users may purchase pre-made, ready-to-transfect minicircle DNA only. SBI also offers custom parental plasmid cloning and minicircle ...
https://systembio.com/shop/mc-easy-minicircle-dna-production-kit-includes-zycy10p3s2t-producer-e-coli-strain/
The American Journal of Tropical Medicine and Hygiene | The Replication of Kinetoplast DNAThe American Journal of Tropical Medicine and Hygiene | The Replication of Kinetoplast DNA
Abstract Kinetoplast DNA is the mitochondrial DNA of trypanosomatids. This DNA consists primarily of thousands of small minicircles which are linked together to form a giant network. Replication of this DNA involves release of individual minicircles from the network to form free minicircles. The free minicircles then replicate and the two progeny are reattached to the network. When all minicircles within the network have replicated, the double-sized network divides to form two progeny structures which are each identical to the parent network.
http://www.ajtmh.org/content/journals/10.4269/ajtmh.1980.29.1064
Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis | BMC...Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis | BMC...
Many trypanosomatid protozoa are important human or animal pathogens. The well defined morphology and precisely choreographed division of trypanosomatid cells makes morphological analysis a powerful tool for analyzing the effect of mutations, chemical insults and changes between lifecycle stages. High-throughput image analysis of micrographs has the potential to accelerate collection of quantitative morphological data. Trypanosomatid cells have two large DNA-containing organelles, the kinetoplast (mitochondrial DNA) and nucleus, which provide useful markers for morphometric analysis; however they need to be accurately identified and often lie in close proximity. This presents a technical challenge. Accurate identification and quantitation of the DNA content of these organelles is a central requirement of any automated analysis method. We have developed a technique based on double staining of the DNA with a minor groove binding (4, 6-diamidino-2-phenylindole (DAPI)) and a base pair intercalating
https://bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-10-1
Can Eating These Healthy Vegetables Lead to Dementia? - Awakening From AlzheimersCan Eating These Healthy Vegetables Lead to Dementia? - Awakening From Alzheimers
Research has confirmed the important connection between our brain and our colon. When we talk about a gut feeling, its because this connection is genuinely physical.. The brain and the gut are directly connected by the vagus nerve. It extends from the brainstem to the abdomen, and is an open line of communication. Cells in the gut produce the neurotransmitter serotonin.. Research has confirmed the connection between a healthy gut and a reduced risk of Alzheimers disease and dementia,4 as well as how certain gut parasites can compromise brain health.5. In a recent study, scientists transferred gut bacteria from old mice to younger mice. The younger mice developed age-related chronic inflammation as a result.6 Research has helped explain just how this type of long-term inflammation in the gut leads to diminished brain health.7. When dietary factors like lectins make changes to the structure of the gut, especially when they allow bacteria to escape, it results in low-grade inflammation in the ...
http://www.awakeningfromalzheimers.com/can-eating-these-healthy-vegetables-lead-to-dementia/
Policies | Bombus.CoPolicies | Bombus.Co
TERMS & CONDITIONS. www.bombusco.com (the Site) is operated by Bombus.Co (ABN 44 583 510 944) (Bombus.Co or We). When you use the site, you agree to the terms and conditions that follow. These terms and conditions also govern the supply of any goods to you by Bombus.Co whether facilitated by the Site or by any other means. If you do not agree to these terms and conditions, you should immediately cease use of the Site. The use of the Site and the provision of goods to you by Bombus.Co is conditional on your acceptance without modification of the terms and conditions.. ​. ALLERGIES. Bombus.Co will not be responsible for any allergy-related issues. Unless it is specified otherwise on your order, all products may contain or come into contact with dairy, wheat, nuts, soy, and other allergens. It is the clients responsibility to inform their guests of any risks.. ​. RETURN POLICY​. ​. We confirm that in the unlikely event that there is a problem with your order we will refund your ...
https://www.bombusco.com/about-1
EFSA Journal | Europäische Behörde für LebensmittelsicherheitEFSA Journal | Europäische Behörde für Lebensmittelsicherheit
ATVing & Dirt Biking<...ATVing & Dirt Biking<...
If you are looking for track and cross country adventure. The Kootenays offers a Motocross track and cross country off road motorcycle facility, with 18 km of single track. Offering 3 loops for the intermediate to difficult. Located on Beavervale Creek Forest Service Road,on the Bombi Summit 4Km from the Salmo side, 21 km from the Castlegar side. Day Passes available. Check out the West Kootenay Recreational Dirt Bike & ATV Society for more information about membership and scheduled events.. ...
https://www.discovernelson.com/activities/atving-dirt-biking/
Publications | Microbiology Department at UMass Amherst
Morris JC, Drew ME, Klingbeil MM, Motyka SA, Saxowsky TT, Wang Z, Englund PT. 2001. Replication of kinetoplast DNA: an update for the new millennium.. Int J Parasitol. 31(5-6):453-8. ...
https://www.micro.umass.edu/faculty-and-research/publications?page=3&s=title&%3Bamp%3Bo=asc&%3Bamp%3Bf%5Bauthor%5D=232&%3Bf%5Bauthor%5D=593&f%5Bauthor%5D=180
Science
To bring promising anti- trypanosomatid drug discovery initiatives forward with the help of key experts in industry and academia to create a unique and powerful drug discovery platform with the common objective of advancing promising laboratory- driven discoveries into clinical utility. EU FP7 work programme : HEALTH.2013.2.3.4-2: Drug development for neglected parasitic diseases. FP7-HEALTH-2013-INNOVATION-1. Duration: 36 months Scientific oordinator Dr. Jane MacDougall PhD [email protected] ...
http://kindred-fp7.com
CrithidiaCrithidia
C. deanei is atypical of the Crithidia genus, and it has been argued not a member of the Crithidia at all. It is not typical of ... Crithidia mellificae is a parasite of the bee. Crithidia brevicula might incorporate species of the genus Wallaceina ( ... Wikimedia Commons has media related to Crithidia. "Crithidia" at the Encyclopedia of Life (Articles with short description, ... Crithidia is a genus of trypanosomatid Euglenozoa. They are parasites that exclusively parasitise arthropods, mainly insects. ...
https://en.wikipedia.org/wiki/Crithidia
Crithidia fasciculataCrithidia fasciculata
... is a species of parasitic excavates. C. fasciculata, like other species of Crithidia have a single host ... ISBN 0-486-65126-6. Awadelkariem, FM; Hunter, KJ; Kirby, GC; Warhurst, DC (February 1995). "Crithidia fasciculata as Feeder ... "An Insight into the Proteome of Crithidia fasciculata Choanomastigotes as a Comparative Approach to Axenic Growth, Peanut ... and Cellular Adhesion in the Protozoan Parasite Crithidia fasciculata". Current Microbiology. 69 (4): 541-8. doi:10.1007/s00284 ...
https://en.wikipedia.org/wiki/Crithidia_fasciculata
Crithidia otongatchiensisCrithidia otongatchiensis
... is a species of monoxenous trypanosomatid. It is known to parasitise Brachycera flies, and was first ... "Crithidia otongatchiensis" at the Encyclopedia of Life v t e (Articles with short description, Short description is different ...
https://en.wikipedia.org/wiki/Crithidia_otongatchiensis
Crithidia pragensisCrithidia pragensis
... is a species of monoxenous trypanosomatid. It is known to parasitise Brachycera flies, and was first found ...
https://en.wikipedia.org/wiki/Crithidia_pragensis
Crithidia luciliaeCrithidia luciliae
... is a flagellate parasite that uses the housefly, Musca domestica, as a host. As part of the family of ... Hall, ST; Penny, JI; Gero, AM; Krishna, S (October 1998). "Crithidia luciliae: functional expression of nucleoside and ... Slater NG, Cameron JS, Lessof MH (September 1976). "The Crithidia luciliae kinetoplast immunofluorescence test in systemic ...
https://en.wikipedia.org/wiki/Crithidia_luciliae
Doris MackinnonDoris Mackinnon
... and Crithidia hystrighopsyllae, n. sp". Parasitology. 2 (3): 288-296. doi:10.1017/S0031182000001736. Mackinnon, D. L. ( ...
https://en.wikipedia.org/wiki/Doris_Mackinnon
Trypanothione synthaseTrypanothione synthase
Comini M, Menge U, Wissing J, Flohé L (February 2005). "Trypanothione synthesis in crithidia revisited". J. Biol. Chem. 280 (8 ... "Cloning and characterization of the two enzymes responsible for trypanothione biosynthesis in Crithidia fasciculata". J. Biol. ... "Purification of glutathionylspermidine and trypanothione synthetases from Crithidia fasciculata". Protein Sci. 1 (7): 874-83. ...
https://en.wikipedia.org/wiki/Trypanothione_synthase
BumblebeeBumblebee
... s are parasitised by tracheal mites, Locustacarus buchneri; protozoans including Crithidia bombi and Apicystis bombi; ...
https://en.wikipedia.org/wiki/Bumblebee
cytochrome c)-lysine N-methyltransferasecytochrome c)-lysine N-methyltransferase
Valentine J, Pettigrew GW (1982). "A cytochrome c methyltransferase from Crithidia oncopelti". Biochem. J. 201 (2): 329-38. PMC ...
https://en.wikipedia.org/wiki/(cytochrome_c)-lysine_N-methyltransferase
Antinuclear antibodyAntinuclear antibody
Crithidia luciliae are haemoflaggelate single celled protists. They are used as a substrate in immunofluorescence for the ... Slater, NG; Cameron, JS; Lessof, MH (September 1976). "The Crithidia luciliae kinetoplast immunofluorescence test in systemic ...
https://en.wikipedia.org/wiki/Antinuclear_antibody
Angomonas deaneiAngomonas deanei
d'Avila-Levy CM, Santos LO, Marinho FA, Matteoli FP, Lopes AH, Motta MC, Santos AL, Branquinha MH (2008). "Crithidia deanei: ... As more structural and molecular details were studied, the distinction of A. deanei from other Crithidia species became more ... Angomonas deanei was originally described as Crithidia deanei. In 1973, a Brazilian graduate student Aurora Luiza de Moura ... Sousa, M.A. (1991). "Postnuclear kinetoplast in choanomastigotes of Crithidia deanei Carvalho, 1973. Proposal of a new genus". ...
https://en.wikipedia.org/wiki/Angomonas_deanei
Glutathionylspermidine synthaseGlutathionylspermidine synthase
"Purification of glutathionylspermidine and trypanothione synthetases from Crithidia fasciculata". Protein Sci. 1 (7): 874-83. ...
https://en.wikipedia.org/wiki/Glutathionylspermidine_synthase
H. E. CarterH. E. Carter
A novel branched-chain sphingolipid base from Crithidia facsiculata. Biochem. Biophys. Res. Commun. 22: 316-320. Carter, H. E ...
https://en.wikipedia.org/wiki/H._E._Carter
Anti-dsDNA antibodiesAnti-dsDNA antibodies
Crithidia luciliae is a haemoflagellate protist with an organelle known as the kinetoplast. This organelle contains a high ... The Farr assay is one of the only tests available that detects high avidity antibodies (along with Crithidia luciliae) and also ... ISBN 0-7044-2437-1. Slater NG, Cameron JS, Lessof MH (September 1976). "The Crithidia luciliae kinetoplast immunofluorescence ...
https://en.wikipedia.org/wiki/Anti-dsDNA_antibodies
Bombus muscorumBombus muscorum
The presence of Crithidia bombi has been found to be higher in populations with lower genetic diversity. As B. muscorum ... B. muscorum may contract infections of Crithidia bombi, a trypanosome parasite, from infected nest mates or from others while ... Yourth, Christopher P; Schmid-Hempel, Paul (2006-03-22). "Serial passage of the parasite Crithidia bombi within a colony of its ... Infections have been linked to reduced individual and colony fitness, but the specific relationship between Crithidia bombi and ...
https://en.wikipedia.org/wiki/Bombus_muscorum
Two-spotted bumble beeTwo-spotted bumble bee
B. bimaculatus can also be infected by Crithidia bombi and Apicystis bombi. Both are protozoans, but C. bombi is known to ...
https://en.wikipedia.org/wiki/Two-spotted_bumble_bee
Jaenimonas drosophilaeJaenimonas drosophilae
Additionally, Crithidia trypanosomes are important parasites of Bumblebees implicated in colony collapse disorder. Hamilton; et ...
https://en.wikipedia.org/wiki/Jaenimonas_drosophilae
WallaceinaWallaceina
It is therefore suggested to reassign Wallaceina species either to Crithidia brevicula (for Wallaceina brevicula, W. inconstans ... Crithidia, Leptomonas, Herpetomonas, and Rhynchoidomonas. Wallaceina is characterized by endomastigote morphological forms, ... "Morphological Discordance of the New Trypanosomatid Species Phylogenetically Associated with the Genus Crithidia". Protist. 159 ...
https://en.wikipedia.org/wiki/Wallaceina
LeptomonasLeptomonas
In addition to Leptomonas, one-host trypanosomatids from insects have been traditionally placed in genera Crithidia, ... "Morphological Discordance of the New Trypanosomatid Species Phylogenetically Associated with the Genus Crithidia". Protist. 159 ...
https://en.wikipedia.org/wiki/Leptomonas
BlastocrithidiaBlastocrithidia
Laird, Marshall (1959-10-01). "Blastocrithidia n.g. (Mastigophora: Protomonadina) for Crithidia (in part), with a subarctic ... one-host trypanosomatids from hemipteran and dipteran insects have been traditionally placed in genera Crithidia, Leptomonas, ... "Morphological Discordance of the New Trypanosomatid Species Phylogenetically Associated with the Genus Crithidia". Protist. 159 ...
https://en.wikipedia.org/wiki/Blastocrithidia
Strigomonas culicisStrigomonas culicis
"Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia culicis and Crithidia oncopelti". The ...
https://en.wikipedia.org/wiki/Strigomonas_culicis
Irene McCullochIrene McCulloch
Her dissertation at UC Berkeley compared the life cycle Crithidia and Trypanosoma within invertebrate hosts. When McCulloch ... A comparison of the life cycle of Crithidia with that of Trypanosoma in the invertebrate host. Berkeley: [s.n.] doi:10.5962/bhl ...
https://en.wikipedia.org/wiki/Irene_McCulloch
Bombus hortorumBombus hortorum
... serve as hosts for Crithidia bombi, a widespread gut parasite that is present in many bumblebee species. The ... While inside their host bodies, Crithidia bombi have been discovered to reproduce clonally as well as sexually. After being ... and multiple hosts prevent local adaptation of Crithidia bombi, a parasite of bumblebees (Bombus spp.)". Ecology and Evolution ...
https://en.wikipedia.org/wiki/Bombus_hortorum
Bombus vosnesenskiiBombus vosnesenskii
For the Bombus vosnesenskii, parasitic organisms include the phoretic mite species Kuzinia and the protozoan Crithidia bombi. ...
https://en.wikipedia.org/wiki/Bombus_vosnesenskii
Marguerite LwoffMarguerite Lwoff
She thus showed that hematin, a chemical substance, could substitute to blood for nutrition of Crithidia fasciculata. This ...
https://en.wikipedia.org/wiki/Marguerite_Lwoff
LeishmaniaLeishmania
The four genera Leptomonas, Crithidia, Leishmania, and Endotrypanum form the terminal branches, suggesting a relatively recent ...
https://en.wikipedia.org/wiki/Leishmania
JaenimonasJaenimonas
... is a genus of trypanosomatid parasite that infects mushroom-feeding Drosophila, similar to Crithidia parasites of ...
https://en.wikipedia.org/wiki/Jaenimonas
Andrew G. CampbellAndrew G. Campbell
... complementation of an Escherichia coli ribonuclease H mutation by a cloned genomic fragment from the trypanosomatid Crithidia ...
https://en.wikipedia.org/wiki/Andrew_G._Campbell
Donald GanemDonald Ganem
... and Crithidia". PLOS ONE. 6 (6): e20656. doi:10.1371/journal.pone.0020656. ISSN 1932-6203. PMC 3110205. PMID 21687739. "Beverly ...
https://en.wikipedia.org/wiki/Donald_Ganem
Trypanothione-disulfide reductaseTrypanothione-disulfide reductase
... including those from Crithidia fasciculata, Leishmania infantum, Trypanosoma brucei and Trypanosoma cruzi. The structures ... "Purification and characterization of trypanothione reductase from Crithidia fasciculata, a newly discovered member of the ...
https://en.wikipedia.org/wiki/Trypanothione-disulfide_reductase
Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia...Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia...
COMBINATION OF THE ESSENTIAL OIL CONSTITUENTS CITRAL, EUGENOL AND THYMOL ENHANCE THEIR INHIBITORY EFFECT ON CRITHIDIA ... eugenol and thymol enhance their inhibitory effect on Crithidia fasciculata and Trypanosoma cruzi growth. Revista Brasileira de ... on the proliferation of the trypanosomatids Crithidia fasciculata and Trypanosoma cruzi. The constituents were initially added ...
https://www.arca.fiocruz.br/handle/icict/15601
Figure 2 - Cutaneous Leishmaniasis Caused by an Unknown Leishmania Strain, Arizona, USA - Volume 27, Number 6-June 2021 -...
Sequences of Crithidia fasciculata and Leptomonas seymouri are included as references. L. (V.) panamensis (GenBank accession no ... KP717894); and Crithidia fasciculata (accession no. Y00055). The 2 non-Leishmania trypanosomatids (Leptomonas seymore and ... Sequences from Crithidia fasciculata and Leptomonas seymouri were included as references. Numbers along branches indicate ... Crithidia fasciculata) were included in the phylogenetic tree because they were previously described as co-infecting parasites ...
https://wwwnc.cdc.gov/eid/article/27/6/20-4198-f2
Characterization of kinetoplast minicircle DNA in the lower trypanosomatid Crithidia oncopelti<...
Characterization of kinetoplast minicircle DNA in the lower trypanosomatid Crithidia oncopelti. Dmitriy G. Pestov, Larisa A. ... Characterization of kinetoplast minicircle DNA in the lower trypanosomatid Crithidia oncopelti. / Pestov, Dmitriy G.; Gladkaya ... Characterization of kinetoplast minicircle DNA in the lower trypanosomatid Crithidia oncopelti. In: Molecular and Biochemical ... The kinetoplast DNA of the lower trypanosomatid Crithidia oncopelti contains minicircles of four major size classes. Attempts ...
https://www.researchwithrowan.com/en/publications/characterization-of-kinetoplast-minicircle-dna-in-the-lower-trypa
CURE ExamplesCURE Examples
Biochemistry of Crithidia fasciculata Amy Greene, Albright College. Students in my second-semester biochemistry lab will study ... the biochemistry of the flagellated parasite Crithidia fasciculata. Students in Paul Ulrichs CURE at Georgia State generate C ...
https://serc.carleton.edu/curenet/institutes/ccri/examples.html
ApidologieApidologie
European propolis is highly active against trypanosomatids including Crithidia fasciculata. Abdullah Alotaibi, Godwin U. ...
https://www.apidologie.org/component/citedby/?task=crossref&doi=10.1051/apido/2010016
Clinical Chemistry and Laboratory Medicine (CCLM) Volume 56 Issue 1Clinical Chemistry and Laboratory Medicine (CCLM) Volume 56 Issue 1
To assess anti-dsDNA antibodies, the Crithidia luciliae -based indirect immunofluorescence test (CLIFT) is one of the assays ... Performance analysis of automated evaluation of Crithidia luciliae-based indirect immunofluorescence tests in a routine setting ...
https://www.degruyter.com/journal/key/cclm/56/1/html
Volume 49 Issue 6 | Environmental EntomologyVolume 49 Issue 6 | Environmental Entomology
... infected with the gut parasites Crithidia bombi (Lipa & Triggiani) (Trypanosomatida: Trypanosomatidae) and Nosema ceranae ( ...
https://bioone.org/journals/environmental-entomology/volume-49/issue-6
Carla Santos - Articles - Scientific Research PublishingCarla Santos - Articles - Scientific Research Publishing
Crithidia deanei infection in normal and dexamethasone-immunosuppressed Balb/c mice (Articles) ...
https://scirp.org/journal/articles.aspx?searchCode=Carla+Santos&searchField=authors&page=1
US20040092467A1 - Biological carriers for the delivery of proteins or peptides - Google Patents
241000222716 Crithidia Species 0.000 claims description 8 * 108020005203 Oxidases Proteins 0.000 claims description 8 ...
https://patents.google.com/patent/US20040092467A1/en
Replication Protein A Presents Canonical Functions and Is Also Involved in the Differentiation Capacity of Trypanosoma cruzi |...Replication Protein A Presents Canonical Functions and Is Also Involved in the Differentiation Capacity of Trypanosoma cruzi |...
In 1992, Brown and collaborators purified the RPA complex from Crithidia fasciculata and demonstrated that RPA is a nuclear ... Conservation of structure and function of DNA replication protein A in the trypanosomatid Crithidia fasciculata. Proc Natl Acad ...
https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005181
Infliximab improves signs and symptoms of psoriatic arthritis: results of the IMPACT 2 trial | Annals of the Rheumatic DiseasesInfliximab improves signs and symptoms of psoriatic arthritis: results of the IMPACT 2 trial | Annals of the Rheumatic Diseases
Crowe W, Kushner I. An immunofluorescent method using Crithidia luciliae to detect antibodies to double-stranded DNA. Arthritis ...
https://ard.bmj.com/content/64/8/1150?ijkey=22e70d15dbae13d5e8328e66670514e151a98e90&keytype2=tf_ipsecsha
Publications - Professor Matthew PickeringPublications - Professor Matthew Pickering
DNA and Crithidia. Functional analysis indicated that one subclass of antibodies bound strongly to decondensed DNA areas in ...
https://www.imperial.ac.uk/people/matthew.pickering/publications.html
CZCZ
Molecular mechanisms of thermal resistance of the insect trypanosomatid Crithidia thermophilal. Aygul Ishemgulova , Anzhelika ...
https://prf.osu.cz/kbe/pavel-flegontov/65957/
WHO EMRO | Immunoinflammatory markers and disease activity in systemic lupus erythematosus: something old, something new |...
... and anti-dsDNA were detected by indirect immunofluorescent assay on mouse kidney and stomach slides and Crithidia luciliae ...
https://www.emro.who.int/emhj-volume-16-2010/volume-16-issue-8/article-12.html
Find Research outputs - Augusta University Research Profiles
Plus itPlus it
Double-stranded DNA antibodies were measured using the Crithidia luciliae method and titers ≥ 1:10 were considered positive. ...
https://www.jrheum.org/content/41/9/1834
Find Research outputs - Discovery - the University of Dundee Research Portal
Brandywine hosts annual undergraduate research showcase EURECA | Penn State Brandywine
"Location and Function of Dynamin-Like Protein in Crithidia Fasciculata." ...
https://www.brandywine.psu.edu/story/1030/2014/04/25/brandywine-hosts-annual-undergraduate-research-showcase-eureca
TRYPANOSOMA CRUZI OLEATE DESATURASE: MOLECULAR CHARACTERIZATION AND COMPARATIVE ANALYSIS IN OTHER TRYPANOSOMATIDS | Journal of...TRYPANOSOMA CRUZI OLEATE DESATURASE: MOLECULAR CHARACTERIZATION AND COMPARATIVE ANALYSIS IN OTHER TRYPANOSOMATIDS | Journal of...
Crithidia fasciculata, Herpetomonas megaseliae, Leptomonas seymouri, Trypanosoma freitasi, Trypanosoma rangeli, Trypanosoma ...
https://meridian.allenpress.com/journal-of-parasitology/article-abstract/92/5/1064/3871/TRYPANOSOMA-CRUZI-OLEATE-DESATURASE-MOLECULAR?redirectedFrom=fulltext
DataCite SearchDataCite Search
We inoculated individual bumblebees with Crithidia bombi, an intestinal parasite, and tested effects of eight naturally ...
https://search.datacite.org/works?resource-type-id=dataset&affiliation-id=ror.org%2F0072zz521®istered=2015
Figure 2 - Cutaneous Leishmaniasis Caused by an Unknown Leishmania Strain, Arizona, USA - Volume 27, Number 6-June 2021 -...
Sequences of Crithidia fasciculata and Leptomonas seymouri are included as references. L. (V.) panamensis (GenBank accession no ... KP717894); and Crithidia fasciculata (accession no. Y00055). The 2 non-Leishmania trypanosomatids (Leptomonas seymore and ... Sequences from Crithidia fasciculata and Leptomonas seymouri were included as references. Numbers along branches indicate ... Crithidia fasciculata) were included in the phylogenetic tree because they were previously described as co-infecting parasites ...
https://wwwnc-origin.cdc.gov/eid/article/27/6/20-4198-f2
Find Research outputs - Albert Einstein College of MedicineFind Research outputs - Albert Einstein College of Medicine
4-Aminopyrimidine - LKT Labs4-Aminopyrimidine - LKT Labs
Rembold H. [Inhibition of Crithidia growth by 4-aminopyrimidine]. Hoppe Seylers Z Physiol Chem. 1964;339(1):258-259. PMID: ...
https://lktlabs.com/product/4-aminopyrimidine/
RETRACTED ARTICLE: Universal minicircle sequence binding protein of Leishmania donovani regulates pathogenicity by controlling...RETRACTED ARTICLE: Universal minicircle sequence binding protein of Leishmania donovani regulates pathogenicity by controlling...
The RNAi study in Crithidia spp. showed the post-replication functions of UMSBP [17]. In L. donovani, UMSBPs post-replication ... We observed that LdUMSBP binds with UMS in its reduced form, not in oxidized form (Additional file 1: Figure S4). In Crithidia, ... Tzfati Y, Abeliovich H, Kapeller I, Shlomai J. A single-stranded DNA-binding protein from Crithidia fasciculata recognizes the ... In Crithidia fasciculata, UMSBP binds specifically to the UMS and initiates kDNA replication [11, 12] and this UMS-binding ...
https://cellandbioscience.biomedcentral.com/articles/10.1186/s13578-016-0072-z
Enterokinase Cleavage Capture Kit | 69067
2005) Trypanothione synthesis in Crithidia revisited. Journal of Biological Chemistry 280, 6850-6860. ...
https://www.emdmillipore.com/PR/en/product/Enterokinase-Cleavage-Capture-Kit,EMD_BIO-69067
Extension entomologists, graduate students honored by entomological societyExtension entomologists, graduate students honored by entomological society
Her poster was titled "Molecular survey for the honeybee Trypanosome parasites crithidia mellificae and Lotmaria passim." ...
https://www.uaex.uada.edu/media-resources/news/2017/april2017/04-05-2017-Ark-enotomology-awards.aspx
Can Sunflowers Help Defeat the Insect Apocalypse? | Center for Agriculture, Food, and the Environment at UMass AmherstCan Sunflowers Help Defeat the 'Insect Apocalypse'? | Center for Agriculture, Food, and the Environment at UMass Amherst
... including Crithidia bombi. Since insect pollinators are annually responsible for ecosystem services estimated at well over $200 ...
https://ag.umass.edu/cafe/news/can-sunflowers-help-defeat-insect-apocalypse
Code System Concept
Crithidia (organism). Code System Preferred Concept Name. Crithidia (organism). Concept Status. Published. ...
https://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=56700008
FasciculataBombiMellificaeImmunofluorescenceParasiteELISAInfectionGrowthLuciliaeTrypanosomaIndirect immunofluorescenceLeishmaniaLeptomonasTubulin PolyglutamylaseParasitesOncopeltiTrypanosomeIsolationMosquitoesFound
Fasciculata7
  • L.M. Perry, Myrtaceae (clove) and Thymus vulgaris L., Lamiaceae (thyme), on the proliferation of the trypanosomatids Crithidia fasciculata and Trypanosoma cruzi. (fiocruz.br)
  • Sequences of Crithidia fasciculata and Leptomonas seymouri are included as references. (cdc.gov)
  • and Crithidia fasciculata (accession no. (cdc.gov)
  • The 2 non- Leishmania trypanosomatids ( Leptomonas seymore and Crithidia fasciculata ) were included in the phylogenetic tree because they were previously described as co-infecting parasites in human leishmaniasis cases. (cdc.gov)
  • Students in my second-semester biochemistry lab will study the biochemistry of the flagellated parasite Crithidia fasciculata. (carleton.edu)
  • The role of glutathionylspermidine and trypanothione in regulation of intracellular spermidine levels during growth of Crithidia fasciculata. (dundee.ac.uk)
  • This year's recipient was freshman Marina Mathew for her work titled "Location and Function of Dynamin-Like Protein in Crithidia Fasciculata. (psu.edu)
Bombi3
  • One place of special concern is the way that pathogens have affected, and are devastating, plant pollinators-think of bees, for instance, which have faced waves of die-offs over the past decade, in part due to specific pathogens, including Crithidia bombi. (umass.edu)
  • New research published in the journal Ecology conclusively shows that certain physical traits of flowers affect the health of bumblebees by modulating the transmission of a harmful pathogen called Crithidia bombi. (phys.org)
  • To determine how secondary compound consumption affects survival and pathogen load in Bombus impatiens , we manipulated the presence of a common gut parasite, Crithidia bombi , and dietary concentration of anabasine, a nectar alkaloid produced by Nicotiana spp. (dartmouth.edu)
Mellificae2
  • https://bioviz.org) Annotation and sequence data are from the paper - A draft genome of the honey bee trypanosomatid parasite Crithidia mellificae. (igbquickload.org)
  • 2013) Comprehensive bee pathogen screening in Belgium reveals Crithidia mellificae as a new contributory factor to winter mortality. (prelekara.sk)
Immunofluorescence3
  • Serum antibodies to double-stranded DNA were measured by ELISA, and by Crithidia immunofluorescence. (biomedcentral.com)
  • Kidneys of 13 mice (five Crithidia positive knockout mice and eight controls) were evaluated by light microscopy and immunofluorescence. (biomedcentral.com)
  • That's why AVISE CTD measures ANA by IFA using digital imaging as well as ELISA, and confirms positive ds-DNA results using immunofluorescence Crithidia assay. (exagen.com)
Parasite1
  • Jaenimonas is a genus of trypanosomatid parasite that infects mushroom-feeding Drosophila, similar to Crithidia parasites of Bumblebees. (edayfm.com)
ELISA2
  • Five of the homozygous knockout mice with the highest ELISA titers had IgG anti-dsDNA antibodies as evaluated by Crithidia. (biomedcentral.com)
  • Ganglio Combi MAG ELISA, el screening y monitoreo de neuropatías periféricas más sensible y eficiente: Detección de anticuerpos anti-MAG y anti-gangliósidos en un solo ensayo. (biocientifica.com.ar)
Infection2
  • En règle générale, l'identification du ou des agents pathogènes pouvant causer une infection nécessite des amorces moléculaires spécialisées pour la petite sous-unité de la cassette d'ADNr [11]. (2imanagement.ch)
  • Crithidia infection reduced survival time by more than two days, but this effect was not significant. (dartmouth.edu)
Growth1
  • Rembold H. [Inhibition of Crithidia growth by 4-aminopyrimidine]. (lktlabs.com)
Luciliae3
  • The Food and Drug Administration has granted 510(k) clearance to PerkinElmer for its Euroimmun Crithidia luciliae immunofluorescence test (CLIFT) and CLIFT sensitive assays. (aacc.org)
  • Fourteen of these sera were available for more detailed investigation including the autoantibody profile as determined by several methods including an addressable laser bead assay, Crithidia luciliae indirect immunofluorescence test (CLIFT) and a PM1-Alpha ELISA. (elsevier.com)
  • An ELISA kit and the indirect immunofluorescence method with Crithidia luciliae were used for determining the presence of anti-P ribosomal and anti-dsDNA antibodies, respectively. (finder-articles.com)
Trypanosoma5
  • Sur un trypanosomide nouveau d'une nyctéribie et sur les relations des formes Trypanosoma, Herpetomonas, Leptomonas et Crithidia. (nih.gov)
  • 1948. Primera comprabación de Trypanosoma rangeli en el organisms humana por cultiva sangria perifica. (aavp.org)
  • Reservorios domésticos de Trypanosoma cruzi y Trypanosoma rangeli en Venezuela. (aavp.org)
  • 1995). Kinetoplast maxicircle DNA replication in Crithidia fasciculata and Trypanosoma brucei. (microbiologiaitalia.it)
  • These studies utilize the distinct advantages of Trypanosoma brucei and Crithidia fasciculata to address how mitochondrial shape relates to metabolic adaptation in different host environments. (megpovelab.com)
Indirect immunofluorescence1
  • We used indirect immunofluorescence, the crithidia assay for anti-dsDNA antibodies, and multiple ELISAs for extractable nuclear antigens. (biomedcentral.com)
Leishmania6
  • A set of specific primers based on GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) gene were used to distinguish between Crithidia and Leishmania genera. (nih.gov)
  • The 2 non- Leishmania trypanosomatids ( Leptomonas seymore and Crithidia fasciculata ) were included in the phylogenetic tree because they were previously described as co-infecting parasites in human leishmaniasis cases. (cdc.gov)
  • During the last several decades, researchers have described rare cases of patients co-infected with both Leishmania and other groups of protozoan parasites that usually infect insects, including Crithidia . (nih.gov)
  • We report high-quality draft assemblies and gene annotation for 13 species and/or strains of the protozoan parasite genera Leishmania, Endotrypanum and Crithidia , which span the phylogenetic diversity of the Leishmaniinae sub-family within the kinetoplastid order of the phylum Euglenazoa. (biorxiv.org)
  • Leishmania er en intracellulær protozo som fører til sykdommen leishmaniasis. (uib.no)
  • Pour identifier les espèces de Leishmania , des techniques moléculaires ont été appliquées sur des échantillons prélevés chez 64 patients atteints de leishmaniose cutanée orientés vers l'hôpital régional d'Herat en 2013. (who.int)
Leptomonas3
  • Sequences of Crithidia fasciculata and Leptomonas seymouri are included as references. (cdc.gov)
  • To be able to use this method for further diagnostics, experiments will have to be done comparing the binding specificity and avidity, and testing the similarities of similar genus like Crithidia and Leptomonas, which also produce AAP transporters. (uib.no)
  • More recently, coinfections with Crithidia -like ( 6 ) or Leptomonas- like ( 7 ) parasites have been reported. (cdc.gov)
Tubulin Polyglutamylase1
  • Reinigung und Charakterisierung von Tubulin Polyglutamylase aus Crithidia fasciculata. (mpg.de)
Parasites3
  • Some Crithidia parasites are known to parasitize anopheline mosquitoes. (nih.gov)
  • The current study of parasites isolated from a Brazilian patient confirms that Crithidia parasites also can infect people. (nih.gov)
  • To confirm that these Crithidia parasites could infect mammals, the researchers exposed mice to the parasites isolated from the patient, both intravenously and by injection into the skin, and found that both types of parasite infected the liver. (nih.gov)
Oncopelti1
  • in vitro studies with mammalian tissues and the protozoan Crithidia oncopelti indicate that the drug interferes with nuclear metabolism producing inhibition of the synthesis of DNA, RNA, phospholipids and proteins. (rpsi.ir)
Trypanosome1
  • Flagellar regeneration of the trypanosome Crithidia fasciculata involves post-translational modification of cytoplasmic alpha tubulin. (ox.ac.uk)
Isolation1
  • Isolation of Crithidia spp. (nih.gov)
Mosquitoes3
  • Instead, they were more closely related to Crithidia fasciculata , a parasite that usually colonizes mosquitoes. (nih.gov)
  • They expressed concern that the disease may be mosquito-borne because Anopheles and Culex mosquitoes can host the Crithidia parasite. (nih.gov)
  • We have found that free swimming flagellated cells of Crithidia fasciculata , a kinetoplastid parasite of mosquitoes, can rapidly and dramatically alter their cellular and gene expression programs to resemble the adherent, stationary forms observed in mosquitoes. (megpovelab.com)
Found1
  • Being infected with Crithidia shortens a bee's lifespan, but consuming anabasine doesn't, the researchers found. (conservationmagazine.org)
Clinical Chemistry and Laboratory Medicine (CCLM) Volume 56 Issue 1
Clinical Chemistry and Laboratory Medicine (CCLM) Volume 56 Issue 1 (degruyter.com)
Volume 49 Issue 6 | Environmental Entomology
Volume 49 Issue 6 | Environmental Entomology (bioone.org)
Begriffe mit L | Medizin-Lexikon | gesundheit.de
Begriffe mit L | Medizin-Lexikon | gesundheit.de (gesundheit.de)
Publications - Professor Matthew Pickering
Publications - Professor Matthew Pickering (imperial.ac.uk)
CZ
CZ (prf.osu.cz)
WHO EMRO | Immunoinflammatory markers and disease activity in systemic lupus erythematosus: something old, something new |...
WHO EMRO | Immunoinflammatory markers and disease activity in systemic lupus erythematosus: something old, something new |... (emro.who.int)
Find Research outputs - Augusta University Research Profiles
Find Research outputs - Augusta University Research Profiles (augusta.pure.elsevier.com)
Find Research outputs - Discovery - the University of Dundee Research Portal
Find Research outputs - Discovery - the University of Dundee Research Portal (discovery.dundee.ac.uk)
4-Aminopyrimidine - LKT Labs
4-Aminopyrimidine - LKT Labs (lktlabs.com)
RETRACTED ARTICLE: Universal minicircle sequence binding protein of Leishmania donovani regulates pathogenicity by controlling...
RETRACTED ARTICLE: Universal minicircle sequence binding protein of Leishmania donovani regulates pathogenicity by controlling... (cellandbioscience.biomedcentral.com)
Pb]Doença similar à leishmaniose, porém mais grave, é descoberta no país[:en]Similar but more serious leishmaniasis disease is...
Pb]Doença similar à leishmaniose, porém mais grave, é descoberta no país[:en]Similar but more serious leishmaniasis disease is... (isc.ufba.br)
Can Sunflowers Help Defeat the 'Insect Apocalypse'? | Center for Agriculture, Food, and the Environment at UMass Amherst
Can Sunflowers Help Defeat the 'Insect Apocalypse'? | Center for Agriculture, Food, and the Environment at UMass Amherst (ag.umass.edu)
Index Catalog // Royal Botanic Gardens, Kew research repository
Index Catalog // Royal Botanic Gardens, Kew research repository (kew.iro.bl.uk)
Autoimmunity in IgM-deficient mice | Arthritis Research & Therapy | Full Text
Autoimmunity in IgM-deficient mice | Arthritis Research & Therapy | Full Text (arthritis-research.biomedcentral.com)
Publikationen | Max-Planck-Institut für Multidisziplinäre Naturwissenschaften
Publikationen | Max-Planck-Institut für Multidisziplinäre Naturwissenschaften (mpinat.mpg.de)
Dose-dependent effects of antibiotic intake on Bombus Terrestris (Linnaeus, 1758) dietary intake, survival and parasite...
Dose-dependent effects of antibiotic intake on Bombus Terrestris (Linnaeus, 1758) dietary intake, survival and parasite... (oparu.uni-ulm.de)
Confirmation Test
Confirmation Test (biognost.be)
Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia...
Combination of the essential oil constituents citral, eugenol and thymol enhance their inhibitory effect on Crithidia... (arca.fiocruz.br)
TRYPANOSOMIC - Definition and synonyms of trypanosomic in the English dictionary
TRYPANOSOMIC - Definition and synonyms of trypanosomic in the English dictionary (educalingo.com)
Revue des parasites Nosema chez les abeilles
Revue des parasites Nosema chez les abeilles (2imanagement.ch)
Autoimmunity - Biocientifica
Autoimmunity - Biocientifica (biocientifica.com.ar)
Index Catalog // STAX
Index Catalog // STAX (stax.strath.ac.uk)
ZIB
ZIB (bib.zib.de)
Emerging Parasitic Disease Mimics the Symptoms of Visceral Leishmaniasis in People | NIH: National Institute of Allergy and...
Emerging Parasitic Disease Mimics the Symptoms of Visceral Leishmaniasis in People | NIH: National Institute of Allergy and... (niaid.nih.gov)
IndexCat
IndexCat (indexcat.nlm.nih.gov)