Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Cheek: The part of the face that is below the eye and to the side of the nose and mouth.Food Storage: Keeping food for later consumption.Hibernation: The dormant state in which some warm-blooded animal species pass the winter. It is characterized by narcosis and by sharp reduction in body temperature and metabolic activity and by a depression of vital signs.Nitrosamines: A class of compounds that contain a -NH2 and a -NO radical. Many members of this group have carcinogenic and mutagenic properties.MedlinePlus: NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.GreeceDolphins: Mammals of the families Delphinidae (ocean dolphins), Iniidae, Lipotidae, Pontoporiidae, and Platanistidae (all river dolphins). Among the most well-known species are the BOTTLE-NOSED DOLPHIN and the KILLER WHALE (a dolphin). The common name dolphin is applied to small cetaceans having a beaklike snout and a slender, streamlined body, whereas PORPOISES are small cetaceans with a blunt snout and rather stocky body. (From Walker's Mammals of the World, 5th ed, pp978-9)Cetacea: An order of wholly aquatic MAMMALS occurring in all the OCEANS and adjoining seas of the world, as well as in certain river systems. They feed generally on FISHES, cephalopods, and crustaceans. Most are gregarious and most have a relatively long period of parental care and maturation. Included are DOLPHINS; PORPOISES; and WHALES. (From Walker's Mammals of the World, 5th ed, pp969-70)Porpoises: Mammals of the family Phocoenidae comprising four genera found in the North Pacific Ocean and both sides of the North Atlantic Ocean and in various other seas. They differ from DOLPHINS in that porpoises have a blunt snout and a rather stocky body while dolphins have a beak-like snout and a slender, streamlined body. They usually travel in small groups. (From Walker's Mammals of the World, 5th ed, pp1003-4)Bottle-Nosed Dolphin: The species Tursiops truncatus, in the family Delphinidae, characterized by a bottle-shaped beak and slightly hooked broad dorsal fin.Whales: Large marine mammals of the order CETACEA. In the past, they were commercially valued for whale oil, for their flesh as human food and in ANIMAL FEED and FERTILIZERS, and for baleen. Today, there is a moratorium on most commercial whaling, as all species are either listed as endangered or threatened.Motilin: A peptide of about 22-amino acids isolated from the DUODENUM. At low pH it inhibits gastric motor activity, whereas at high pH it has a stimulating effect.Receptors, Gastrointestinal Hormone: Cell surface proteins that bind gastrointestinal hormones with high affinity and trigger intracellular changes influencing the behavior of cells. Most gastrointestinal hormones also act as neurotransmitters so these receptors are also present in the central and peripheral nervous systems.Receptors, Neuropeptide: Cell surface receptors that bind specific neuropeptides with high affinity and trigger intracellular changes influencing the behavior of cells. Many neuropeptides are also hormones outside of the nervous system.Erythromycin: A bacteriostatic antibiotic macrolide produced by Streptomyces erythreus. Erythromycin A is considered its major active component. In sensitive organisms, it inhibits protein synthesis by binding to 50S ribosomal subunits. This binding process inhibits peptidyl transferase activity and interferes with translocation of amino acids during translation and assembly of proteins.Gastrointestinal Agents: Drugs used for their effects on the gastrointestinal system, as to control gastric acidity, regulate gastrointestinal motility and water flow, and improve digestion.Cisapride: A substituted benzamide used for its prokinetic properties. It is used in the management of gastroesophageal reflux disease, functional dyspepsia, and other disorders associated with impaired gastrointestinal motility. (Martindale The Extra Pharmacopoeia, 31st ed)Pyloric Antrum: The region between the sharp indentation at the lower third of the STOMACH (incisura angularis) and the junction of the PYLORUS with the DUODENUM. Pyloric antral glands contain mucus-secreting cells and gastrin-secreting endocrine cells (G CELLS).In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.Mesocricetus: A genus of the family Muridae having three species. The present domesticated strains were developed from individuals brought from Syria. They are widely used in biomedical research.Cricetulus: A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.Polytene Chromosomes: Extra large CHROMOSOMES, each consisting of many identical copies of a chromosome lying next to each other in parallel.Chromosomes, Artificial, Bacterial: DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.Platypus: A small aquatic oviparous mammal of the order Monotremata found in Australia and Tasmania.Mouth Mucosa: Lining of the ORAL CAVITY, including mucosa on the GUMS; the PALATE; the LIP; the CHEEK; floor of the mouth; and other structures. The mucosa is generally a nonkeratinized stratified squamous EPITHELIUM covering muscle, bone, or glands but can show varying degree of keratinization at specific locations.Reference Books: Books designed by the arrangement and treatment of their subject matter to be consulted for definite terms of information rather than to be read consecutively. Reference books include DICTIONARIES; ENCYCLOPEDIAS; ATLASES; etc. (From the ALA Glossary of Library and Information Science, 1983)Scandentia: An order of the class MAMMALS that consists of one family, TUPAIIDAE (tree shrews), 5 genera (one of which is TUPAIA), and 16 species. Their recent distribution is from India to the Philippines, southern China to Java, Borneo, Sumatra, Bali, and other islands in those regions.Insectivora: An order of insect eating MAMMALS including MOLES; SHREWS; HEDGEHOGS and tenrecs.Streptomyces griseus: An actinomycete from which the antibiotics STREPTOMYCIN, grisein, and CANDICIDIN are obtained.Methyl n-Butyl Ketone: An industrial solvent which causes nervous system degeneration. MBK is an acronym often used to refer to it.Lagomorpha: An order of small mammals comprising two families, Ochotonidae (pikas) and Leporidae (RABBITS and HARES). Head and body length ranges from about 125 mm to 750 mm. Hares and rabbits have a short tail, and the pikas lack a tail. Rabbits are born furless and with both eyes and ears closed. HARES are born fully haired with eyes and ears open. All are vegetarians. (From Nowak, Walker's Mammals of the World, 5th ed, p539-41)Sodium Channels: Ion channels that specifically allow the passage of SODIUM ions. A variety of specific sodium channel subtypes are involved in serving specialized functions such as neuronal signaling, CARDIAC MUSCLE contraction, and KIDNEY function.Chordata: Phylum in the domain Eukarya, comprised of animals either with fully developed backbones (VERTEBRATES), or those with notochords only during some developmental stage (CHORDATA, NONVERTEBRATE).TRPV Cation Channels: A subgroup of TRP cation channels named after vanilloid receptor. They are very sensitive to TEMPERATURE and hot spicy food and CAPSAICIN. They have the TRP domain and ANKYRIN repeats. Selectivity for CALCIUM over SODIUM ranges from 3 to 100 fold.Receptor, Melatonin, MT2: A melatonin receptor subtype primarily found expressed in the BRAIN and RETINA.Isoindoles: Benzopyrroles with the nitrogen at the number two carbon, in contrast to INDOLES which have the nitrogen adjacent to the six-membered ring.Enoplida Infections: Infections with nematodes of the order ENOPLIDA.Ants: Insects of the family Formicidae, very common and widespread, probably the most successful of all the insect groups. All ants are social insects, and most colonies contain three castes, queens, males, and workers. Their habits are often very elaborate and a great many studies have been made of ant behavior. Ants produce a number of secretions that function in offense, defense, and communication. (From Borror, et al., An Introduction to the Study of Insects, 4th ed, p676)Capillaria: A genus of trichuroid nematodes parasitic in the liver and intestines of many mammals and birds. Two species, C. hepatica and C. philippinensis, produce often fatal infections in man.Consumer Health Information: Information intended for potential users of medical and healthcare services. There is an emphasis on self-care and preventive approaches as well as information for community-wide dissemination and use.Social Media: Platforms that provide the ability and tools to create and publish information accessed via the INTERNET. Generally these platforms have three characteristics with content user generated, high degree of interaction between creator and viewer, and easily integrated with other sites.WyomingRodentia: A mammalian order which consists of 29 families and many genera.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Liliaceae: A monocot family within the order Liliales. This family is divided by some botanists into other families such as Convallariaceae, Hyacinthaceae and Amaryllidaceae. Amaryllidaceae, which have inferior ovaries, includes CRINUM; GALANTHUS; LYCORIS; and NARCISSUS and are known for AMARYLLIDACEAE ALKALOIDS.

Fitzgerald factor (high molecular weight kininogen) clotting activity in human plasma in health and disease in various animal plasmas. (1/32376)

Fitzgerald factor (high molecular weight kininogen) is an agent in normal human plasma that corrects the impaired in vitro surface-mediated plasma reactions of blood coagulation, fibrinolysis, and kinin generation observed in Fitzgerald trait plasma. To assess the possible pathophysiologic role of Fitzgerald factor, its titer was measured by a functional clot-promoting assay. Mean +/- SD in 42 normal adults was 0.99+/-0.25 units/ml, one unit being the activity in 1 ml of normal pooled plasma. No difference in titer was noted between normal men and women, during pregnancy, or after physical exercise. Fitzgerald factor activity was significantly reduced in the plasmas of eight patients with advanced hepatic cirrhosis (0.40+/-0.09 units/ml) and of ten patients with disseminated intravascular coagulation (0.60+/-0.30 units/ml), but was normal in plasmas of patients with other congenital clotting factor deficiencies, nephrotic syndrome, rheumatoid arthritis, systemic lupus erythematosus, or sarcoidosis, or under treatment with warfarin. The plasmas of 21 mammalian species tested appeared to contain Fitzgerald factor activity, but those of two avian, two repitilian, and one amphibian species did not correct the coagulant defect in Fitzgerald trait plasmas.  (+info)

Differences in benzo(a)pyrene metabolism between rodent liver microsomes and embryonic cells. (2/32376)

Differences in benzo(a)pyrene metabolite pattern have been shown by rodent liver microsomes (Sprague-Dawley) and rodent embryo cells from Syrian hamsters and NIH Swiss mice. Rodent liver induced by methylcholanthrene shows marked quantitative variation between species. Additional pattern changes were found in mouse and hamster embryo secondary cultures with a reduction of the K-region metabolites and a marked increase in 9-hydroxybenzo(a)-pyrene. These results are indicative of a region-specific attack on the carcinogen by the cell monooxygenases which is distinct from the liver attack of microsomal enzymes on benzo(a)pyrene. These results suggest that activation and detoxification of benzo(a)pyrene may be species and tissue variable, and susceptibility and resistence to malignant transformation may be predicted on induction of a fortuitous combination of intermediate metabolic steps.  (+info)

Ambiguity of the thymidine index. (3/32376)

The observed thymidine indices of seven experimental tumor lines are compared as a function of duration of emulsion exposure. The effects of dose level of tritiated thymidine and background threshold are also evaluated. The results indicate that an arbitrary high background threshold discriminates against "lightly" labeled cells at short periods of exposure but that the chosen threshold becomes less critical with longer exposure. The observed thymidine index increases with increasing duration of emulsion exposure but appears to approach a plateau for all tumor systems. The "thymidine index curves" are significantly different for each tumor. There is an inverse relationship between the dose of tritiated thymidine and the duration of exposure required to recognize the same fraction of cells as labeled in a given tumor. Similar experimental conditions do not necessarily guarantee a valid basis for comparison of observed thymidine indices among tumors.  (+info)

Morphogenesis of callosal arbors in the parietal cortex of hamsters. (4/32376)

The morphogenesis of callosal axons originating in the parietal cortex was studied by anterograde labeling with Phaseolus lectin or biocytin injected in postnatal (P) hamsters aged 7-25 days. Some labeled fibers were serially reconstructed. At P7, some callosal fibers extended as far as the contralateral rhinal fissure, with simple arbors located in the homotopic region of the opposite cortical gray matter, and two or three unbranched sprouts along their trajectory. From P7 to P13, the homotopic arbors became more complex, with branches focused predominantly, but not exclusively, in the supra- and infragranular layers of the homotopic region. Simultaneously, the lateral extension of the trunk axon in the white matter became shorter, finally disappearing by P25. Arbors in the gray matter were either bilaminar (layers 2/3 and 5) or supragranular. A heterotopic projection to the lateral cortex was consistently seen at all ages; the heterotopic arbors follow a similar sequence of events to that seen in homotopic regions. These observations document that callosal axons undergo regressive tangential remodeling during the first postnatal month, as the lateral extension of the trunk fiber gets eliminated. Radially, however, significant arborization occurs in layer-specific locations. The protracted period of morphogenesis suggests a correspondingly long plastic period for this system of cortical fibers.  (+info)

A premature termination codon interferes with the nuclear function of an exon splicing enhancer in an open reading frame-dependent manner. (5/32376)

Premature translation termination codon (PTC)-mediated effects on nuclear RNA processing have been shown to be associated with a number of human genetic diseases; however, how these PTCs mediate such effects in the nucleus is unclear. A PTC at nucleotide (nt) 2018 that lies adjacent to the 5' element of a bipartite exon splicing enhancer within the NS2-specific exon of minute virus of mice P4 promoter-generated pre-mRNA caused a decrease in the accumulated levels of P4-generated R2 mRNA relative to P4-generated R1 mRNA, although the total accumulated levels of P4 product remained the same. This effect was seen in nuclear RNA and was independent of RNA stability. The 5' and 3' elements of the bipartite NS2-specific exon enhancer are redundant in function, and when the 2018 PTC was combined with a deletion of the 3' enhancer element, the exon was skipped in the majority of the viral P4-generated product. Such exon skipping in response to a PTC, but not a missense mutation at nt 2018, could be suppressed by frame shift mutations in either exon of NS2 which reopened the NS2 open reading frame, as well as by improvement of the upstream intron 3' splice site. These results suggest that a PTC can interfere with the function of an exon splicing enhancer in an open reading frame-dependent manner and that the PTC is recognized in the nucleus.  (+info)

In vivo chaperone activity of heat shock protein 70 and thermotolerance. (6/32376)

Heat shock protein 70 (Hsp70) is thought to play a critical role in the thermotolerance of mammalian cells, presumably due to its chaperone activity. We examined the chaperone activity and cellular heat resistance of a clonal cell line in which overexpression of Hsp70 was transiently induced by means of the tetracycline-regulated gene expression system. This single-cell-line approach circumvents problems associated with clonal variation and indirect effects resulting from constitutive overexpression of Hsp70. The in vivo chaperone function of Hsp70 was quantitatively investigated by using firefly luciferase as a reporter protein. Chaperone activity was found to strictly correlate to the level of Hsp70 expression. In addition, we observed an Hsp70 concentration dependent increase in the cellular heat resistance. In order to study the contribution of the Hsp70 chaperone activity, heat resistance of cells that expressed tetracycline-regulated Hsp70 was compared to thermotolerant cells expressing the same level of Hsp70 plus all of the other heat shock proteins. Overexpression of Hsp70 alone was sufficient to induce a similar recovery of cytoplasmic luciferase activity, as does expression of all Hsps in thermotolerant cells. However, when the luciferase reporter protein was directed to the nucleus, expression of Hsp70 alone was not sufficient to yield the level of recovery observed in thermotolerant cells. In addition, cells expressing the same level of Hsp70 found in heat-induced thermotolerant cells containing additional Hsps showed increased resistance to thermal killing but were more sensitive than thermotolerant cells. These results suggest that the inducible form of Hsp70 contributes to the stress-tolerant state by increasing the chaperone activity in the cytoplasm. However, its expression alone is apparently insufficient for protection of other subcellular compartments to yield clonal heat resistance to the level observed in thermotolerant cells.  (+info)

Requirement for transcription factor NFAT in interleukin-2 expression. (7/32376)

The nuclear factor of activated T cells (NFAT) transcription factor is implicated in expression of the cytokine interleukin-2 (IL-2). Binding sites for NFAT are located in the IL-2 promoter. Furthermore, pharmacological studies demonstrate that the drug cyclosporin A inhibits both NFAT activation and IL-2 expression. However, targeted disruption of the NFAT1 and NFAT2 genes in mice does not cause decreased IL-2 secretion. The role of NFAT in IL-2 gene expression is therefore unclear. Here we report the construction of a dominant-negative NFAT mutant (dnNFAT) that selectively inhibits NFAT-mediated gene expression. The inhibitory effect of dnNFAT is mediated by suppression of activation-induced nuclear translocation of NFAT. Expression of dnNFAT in cultured T cells caused inhibition of IL-2 promoter activity and decreased expression of IL-2 protein. Similarly, expression of dnNFAT in transgenic mice also caused decreased IL-2 gene expression. These data demonstrate that NFAT is a critical component of the signaling pathway that regulates IL-2 expression.  (+info)

Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis. (8/32376)

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences contained within a YAC by using cDNA representational difference analysis (RDA) to directly isolate transcripts expressed from the YAC in mammalian cells. The RDA tester cDNAs were generated from a previously reported hamster cell line derived by stable transfer of a 590-kb YAC (911D5) that expressed NPC1, the human gene responsible for Niemann-Pick type C (NP-C). The driver cDNAs were generated from a control hamster cell line that did not contain the YAC that expressed NPC1. Among the gene fragments obtained by RDA, NPC1 was the most abundant product. In addition, two non-NPC1 fragments were isolated that were mapped to and expressed from 911D5. One of these RDA gene fragments (7-R) spans more than one exon and has 98% sequence identity with a human cDNA clone reported previously as an expressed sequence tag (EST), but not mapped to a chromosomal region. The other fragment (2-R) that had no significant sequence similarities with known mammalian genes or ESTs, was further localized to the region of overlap between YACs 911D5 and 844E3. The latter YAC is part of a contig across the NP-C candidate region, but does not contain NPC1. This two-part approach in which stable YAC transfer is followed by cDNA RDA should be a useful adjunct strategy to expedite the cloning of human genes when a YAC contig is available across a candidate interval.  (+info)

About 10% of the Chinese population are chronic carriers of hepatitis B virus (HBV). Thus, the development of a highly efficient process for the preparation of a vaccine based on a recombinant hepatitis B surface antigen (HBsAg) is very important to the Chinese national immunization program. To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1% poly(ethylene glycol) (PEG 10,000) to the mobile phase. Furthermore, based on analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the intact glycoprotein form of CHO-HBsAg was completely preserved by the addition of PEG. In the absence of PEG the glycoprotein form of CHO-HBsAg was also spread out into the high salt elution fraction. High-performance size-exclusion chromatography with on-line multiangle-laser-light scattering (HPSEC-MALLS) analysis was performed to monitor the status of the ...
N,N,N,N-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 1000, 2500 and 5000 micrograms/mL in both with and without activation. It produced a positive response in this system with or without metabolic activation, but only at the highest concentration 5,000 micrograms/mL However, according to the OECD guidelines TG 473, the compound is considered to be negative in the CHO chromosomal aberration assay, since the compound is not clastogenic at 0.01M (1,140 micrograms/mL). A confirmatory chromosome aberration assay was performed without activation also showed negative at concentrations up to 3,000 micrograms/mL but positive at the highest concentration.
We have isolated three independent Chinese hamster ovary cell mutants (B3853, I223, and M311) with temperature-sensitive, pleiotropic defects in receptor-mediated endocytosis. Activities affected at 41 degrees C include uptake via the D-mannose 6-phosphate receptor, accumulation of Fe from diferric transferrin, uptake of alpha 2-macroglobulin, compartmentalization of newly synthesized acid hydrolases, resistance to ricin, and sensitivity to diphtheria and Pseudomonas toxins and modeccin. The three mutants also displayed decreased sialylation of some secreted glycoproteins at 41 degrees C, reminiscent of the nonconditional mutant DTG1-5-4 that showed both endocytic and Golgi-associated defects (Robbins, A.R., C. Oliver, J.L. Bateman, S.S. Krag, C.J. Galloway, and I. Mellman, 1984, J. Cell Biol., 99:1296-1308). Phenotypic changes were detectable within 30 min after transfer of the mutants to 41 degrees C; maximal alteration of most susceptible functions was obtained 4 h after temperature shift. At ...
L-Histidine markedly increased the growth- and DNA synthesis-inhibitory effects elicited by hydrogen peroxide in cultured Chinese hamster ovary cells. DNA single-strand breakage was also higher in the presence of the amino acid and, in addition, these breaks were characterized by a slower rate of repair, compared with that of the breaks generated by the oxidant alone. In the presence of L-histidine, hydrogen peroxide also produced DNA double-strand breakage, a lesion that cannot be detected in cells treated with even exceedingly high concentrations of the oxidant alone. Data reported herein suggest that the L-histidine-mediated increase of the cytotoxic response of cultured Chinese hamster ovary cells to hydrogen peroxide may be at least partially dependent on the formation of DNA double-strand breaks. ...
COUPLING OF MUSCARINIC M1, M2 AND M3 ACETYLCHOLINE-RECEPTORS, EXPRESSED IN CHINESE-HAMSTER OVARY CELLS, TO PERTUSSIS-TOXIN-SENSITIVE INSENSITIVE GUANINE-NUCLEOTIDE-BINDING ...
Cabral, F; Gottesman, M M.; Zimmerman, S B.; and Steinert, P M., "Intermediate filaments from chinese hamster ovary cells contain a single protein. Comparison with more complex systems from baby hamster kidney and mouse epidermal cells." (1981). Subject Strain Bibliography 1981. 19 ...
BioAssay record AID 51937 submitted by ChEMBL: K+ channel blocking activity in Chinese hamster ovary cells expressing HERG Kv11.1.
Mitotic Spindle Proteomics in Chinese Hamster Ovary Cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The Antibody Labs proprietary cell line development technology enables drug developers to move seamlessly from preclinical discovery to manufacture of the biologic for clinical testing. BESTcell clonal Chinese hamster ovary cell lines can be rapidly generated to enable preclinical testing of multiple biologic drug candidates. After selection of the final candidate, the respective cell line can be used to manufacture master cell banks. This revolutionary approach shortens timelines and reduces the reproducibility risk associated with changing the source of the biologic during research and development.
Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100 Anti-Chinese Hamster Ovary Cell Host Cell Proteins (CHO-HCPs) IgG, aff pure Antibodies 800-140-11A-100
Recombinant Mouse Itga4&Itgb1 (Accession # AAH68313 (Integrin alpha 4) & P09055 (Integrin beta 1)) was produced in Chinese Hamster Ovary cell line, CHO-derived.
Eukaryotic expression vectors have been used successfully in viral LT-expressing cell lines (ie. COS) to clone cDNAs encoding proteins that can be detected through their bio-activity or reactivity with specific antibodies. Since Chinese hamster ovary cells (CHO) have been used extensively for the is... DRIVER (Chinese) ...
Is there anybody who get a stabile transformation with the pcDNA3 vector (INVITROGEN) in BHK21 (baby hamster kidney) cells and what was the level of overexpression compared to a normal cell line expressing the inserted cDNA? Thanks Dietmar ...
The purpose of this study is to assess the safety, pharmacokinetic and activity profiles of the ch14.18 antibody produced in cells of hamster origin (ch
mutant moments e.p. (2020 reissue) by soft cell buy online at resident, to celebrate 40 years since the release of their first ever recording, soft
BioAssay record AID 41661 submitted by ChEMBL: Beta-3 agonist efficacy in an adenylate cyclase assay performed on chinese hamster ovary cells transfected with human Beta-3 adrenergic receptor; Inactive.
TY - JOUR. T1 - Dietary D-allulose alters cholesterol metabolism in Golden Syrian hamsters partly by reducing serum PCSK9 levels. AU - Kanasaki, Akane. AU - Jiang, Zhe. AU - Mizokami, Takuya. AU - Shirouchi, Bungo. AU - Iida, Tetsuo. AU - Nagata, Yasuo. AU - Sato, Masao. PY - 2019/9/1. Y1 - 2019/9/1. N2 - D-Allulose, a C-3 epimer of D-fructose, is a rare sugar reported to be a non-caloric sweetener having several health beneficial effects including anti-hyperglycemia and anti-obesity. However, the impact of dietary D-allulose on cholesterol metabolism remains unclear. Therefore, we studied the effects of D-allulose on the cholesterol metabolism of Golden Syrian hamsters, an animal model with a lipid metabolism similar to that of humans. Hamsters received either normal diet (ND) or high-fat diet (HFD) with or without 3% D-allulose for 4 or 8 weeks. While there were no significant differences in total serum cholesterol levels between the groups, D-allulose significantly increased HDL-cholesterol ...
Phodopus campbelli is very small in size and the pelage is short and silky. The underside of the animal is covered in soft, buff, light grey fur and the dorsal portions, including the head, are woody brown in color. The underfur is quite short and is a dark slate grey. A defined charcoal stripe runs from between the ears to the tail. The pads of all digits, and the small tail, are covered in silky white fur. Additionally, Campbells hamsters, like other members of the subfamily Cricetinae, possess large internal cheek pouches that terminate above the scapula. Males are larger than females. (Thomas, 1905). Phodopus campbelli is often confused with Phodopus sungorus, Siberian hamsters. However, there are several physical characteristics that distinguish the two species. The ears of Phodopus campbelli are generally smaller than those of Phodopus sungorus. The mid-dorsal stripe of the Campbells hamster is both thin and defined and the area where the dorsal fur meets the ventral fur is a creamy ...
TY - JOUR. T1 - Effect of cholesterol feeding on DNA damage in male and female Syrian hamsters. AU - Turley, E. AU - Armstrong, NC. AU - Wallace, JMW. AU - Gilmore, WS. AU - McKelvey-Martin, VJ. AU - Allen, J. AU - Strain, JJ. PY - 1999/1. Y1 - 1999/1. N2 - Cholesterol oxides are cytotoxic and have been implicated in many disease processes; however, it has been proposed that cholesterol oxides result from cholesterol acting as a sacrificial antioxidant. In this study, the effect of dietary cholesterol on DNA damage, assessed by the alkaline comet assay, was examined in male and female Syrian hamsters. Animals were fed ad libitum a modified AIN-76 diet (control) or a diet with 0.5% cholesterol for 10 weeks. Following the 10-week feeding period, there was no significant difference in body weight between cholesterol-fed and control animals. Cholesterol feeding resulted in significant liver hypertrophy, and increased plasma total and HDL cholesterol in both male and female animals compared with ...
The role of polyamines in macromolecular synthesis has been studied using the synthesis of Semliki-Forest virus (SF virus) in normal and alpha-difluoromethylornithine-treated baby-hamster kidney (BHK21) cells as a model system. The activities of ornithine decarboxylase and S-adenosylmethionine decarboxylase, the rate-limiting enzymes in polyamine biosynthesis, decreased rapidly in mock- and SF-virus-infected cells, indicating that virus production in BHK21 cells was not dependent on polyamines formed after infection. A prolonged treatment of BHK21 cells with alpha-difluoro-methylornithine, a specific inhibitor of polyamine synthesis, resulted in a marked inhibition of the initial rate of virus production, which appeared 72 h after the beginning of the treatment. This inhibition was reversed by putrescine, spermidine and spermine, and at last partially by several other diamines and polyamine homologues. Polyamine-depletion also markedly reduced viral RNA polymerase activity in SF-virus infected ...
TY - JOUR. T1 - Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. AU - Thompson, L. H.. AU - Busch, D. B.. AU - Brookman, K.. AU - Mooney, C. L.. AU - Glaser, D. A.. PY - 1981. Y1 - 1981. N2 - Mutant lines of Chinese hamster ovary cells that show hypersensitivity to killing and mutagenesis by UV light were analyzed by genetic complementation analysis to determine whether defects in different gene loci might underlie a common cellular phenotype. To facilitate rapid screening of mutant clones, a procedure was devised that allowed presumptive complementation to be assessed on the basis of the frequency of UV-resistant cells after fusion by polyethylene glycol. Four classes were identified among 44 clones tested. By using drug-resistance markers for selection of hybrid cells in crosses between UV mutant and wild type, a mutant from each of the four classes was shown to behave as phenotypically recessive. Hybrids were also isolated from crosses between each of the ...
Human apolipoprotein (apo) A-I is secreted as a proprotein of 249 amino acids and is processed extracellularly to the mature form (243 amino acids) by removal of a six-residue propeptide segment. We have examined the role of the apoA-I propeptide in intracellular transport and secretion using transfected baby hamster kidney cells that secreted either proapoA-I (from the wild-type cDNA, A-Iwt) or mature-form apoA-I (from A-I delta pro, a cDNA in which the propeptide sequence was deleted). Deletion of the propeptide from the apoA-I sequence did not affect the rate of apoA-I synthesis, nor did it affect the fidelity of proteolytic removal of the prepeptide. However, the propeptide deletion caused mature-form apoA-I to accumulate within the cells as determined by pulse-chase experiments; the intracellular retention times for the mature-form apoA-I in which the propeptide was prematurely removed was three times longer than that of proapoA-I (t1/2 , 3 h compared with approximately 50 min). There was ...
Goat anti-Golden Syrian hamster IgG recognizes Golden Syrian hamster IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Rhodamine. Golden Syrian hamster IgG whole molecule (PAB10592) - Products - Abnova
Rabbit anti-Golden Syrian hamster IgG recognizes Golden Syrian hamster IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. Golden Syrian hamster IgG whole molecule (PAB9233) - Products - Abnova
The effect of hyperosmolarity on transient recombinant protein production in Chinese hamster ovary (CHO) cells was investigated. Addition of 90 mM NaCl to the production medium ProCHO5 increased the volumetric yield of recombinant antibody up to 4-fold relative to transfection in ProCHO5 alone. Volumetric yields up to 50 mg l(-1) were achieved in a 6 day batch culture of 3 l. In addition, hyperosmolarity reduced cell growth and increased cell size. The addition of salt to cultures of transiently transfected CHO cells is a simple and cost-effective method to increase TGE yields in this host. Zhang, Xiaowei; Garcia, Isabel Fernandez; Baldi, Lucia; Hacker, David L.; Wurm, Florian M.
TY - JOUR. T1 - One-Step Enrichment of Intact Glycopeptides From Glycoengineered Chinese Hamster Ovary Cells. AU - Yang, Ganglong. AU - Höti, Naseruddin. AU - Chen, Shao Yung. AU - Zhou, Yangying. AU - Wang, Qiong. AU - Betenbaugh, Michael. AU - Zhang, Hui. PY - 2020/4/17. Y1 - 2020/4/17. N2 - Recently, the glycoproteomic analysis of intact glycopeptides has emerged as an effective approach to decipher the glycan modifications of glycoproteins at the site-specific level. A rapid method to enrich intact glycopeptides is essential for the analysis of glycoproteins, especially for biopharmaceutical proteins. In this study, we established a one-step method for the rapid capture of intact glycopeptides for analysis by mass spectrometry. Compared to the conventional sequential enrichment method, the one-step intact glycopeptide enrichment method reduced the sample preparation time and improved the detection of intact glycopeptides with long sequences or non-polar amino acids. Moreover, an increased ...
TY - CONF. T1 - Engineering amino acid supply pathways in Chinese hamster ovary cells. AU - Ley, Daniel. AU - Lund, Anne Mathilde. AU - Rank, Julie. AU - Kildegaard, Helene Faustrup. AU - Andersen, Mikael Rørdam. N1 - Conference code: 9. PY - 2014. Y1 - 2014. M3 - Poster. T2 - 9th Danish Conference on Biotechnology and Molecular Biology. Y2 - 22 May 2014 through 23 May 2014. ER - ...
Effects of granulocyte-macrophage colony stimulating factor produced in Chinese hamster ovary cells (regramostim), Escherichia coli (molgramostim) and yeast (sargramostim) on priming peripheral blood progenitor cells for use with autologous bone marrow after high-dose chemotherapy.
Download Caffeine-induced alterations in non-histone chromosomal proteins of Chinese hamster ovary cells ebook by Susan Claire HarrisonType: pdf, ePub, zip, txt
Adhering CHO cell culture - posted in Tissue and Cell Culture: Hi, I am totally new to CHO (chinese hamster ovary) cell culture, and to make things worse, I am in charge now of five different mutant CHO cell lines received by donation (4 day-travel and customs) . So the thing is that to not make mistakes I am growing them in a rich Hams F12 medium containing 10% FBS, pen-streptomycin, glutamine, and non essential amino-acids. They grow quite well according to their passage number,...
Graham R, Bhatia H, Yoon S. Consequences of trace metal variability and supplementation on CHO cell culture performance: a review of key mechanisms and considerations. Biotechnol Bioeng. 2019 Aug 12 ...
The parent line of BHK-21(C-13) was derived from baby hamster kidneys of five unsexed, 1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker. Following 84 days of continuous cultivation, interrupted only by an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation.
The parent line of BHK-21(C-13) was derived from baby hamster kidneys of five unsexed, 1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker. Following 84 days of continuous cultivation, interrupted only by an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation.
Used for carcinoma cells, rat skeletal myoblasts, Chinese hamster lung cells, and rat, rabbit, and chicken embryos. Corning™ cellgro™ DMEM/Hams Medium F-12 Mix is based on Hams F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids. X6 500 mL Hams F-12 Medium w L-glut ...
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Proteolytic processing of PA triggers partitioning of the anthrax toxin into lipid rafts. (A) Wild-type CHO cells were incubated for 1 h at 4°C with 500 ng/ml
We constructed comparative cytogenetic maps of the Chinese hamster to mouse, rat and human by fluorescence in-situ hybridization using 36 cDNA clones of mouse, rat, Syrian hamster, Chinese hamster and human functional genes. In this study, 30 out of the 36 genes were newly mapped to Chinese hamster …
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
I am trying to determine the best dose of hygromycin that i can use to select my transfected colonies. (working with chinese hamster cell lines). I have tried several concentrations (from 50-250 microgram per ml) on my host cells and changing the media plus antibiotic every 1-2 days. Each time the media has turned yellow and lots of dead cells but still surviving colonies can be seen. How could we know if the cell death is because of the overgrowth of the culture and consequent bad condition or because of the antibiotic?. ...
First off, a VERY IMPORTANT NOTE. Mulder is a Roborovski hamster, which is the smallest of the dwarf hamsters. When Scully died, he weighed 22g, or 0.78oz; robos are usually less than 30g, or 1.06oz. Other dwarf hamsters are slightly larger, and can weigh up to 2.5oz. Syrian hamsters, on the other hand, usually weigh 5-7oz. This house is a little less than 450 square inches, which is a decent size for a dwarf hamster, but would be entirely too small for a Syrian hamster. If you need a house for a Syrian hamster, I highly recommend that you consider adapting these instructions for a much larger table, such as the Lack coffee table or two or more connected Lack side tables ...
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A research team at the Northeast Agricultural University in Harbin managed to breed three transgenic pigs by injecting fluorescent green protein and a "bunch of other junk" into embryonic pigs, said Professor Liu Zhonghua. Liu wore a fancy white lab coat and had multiple degrees adorning his walls, so we assume he must be pretty smart ...
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Sigma-Aldrich offers abstracts and full-text articles by [Qiang Li, Xianghua Liu, Yanhua Wu, Jian An, Saiyin Hexige, Yichen Ling, Mingjun Zhang, Xianmei Yang, Long Yu].
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Shop a large selection of products and learn more about CD49b Hamster anti-Mouse, BB700, Clone: HMα2, BD Optibuild 50μg; BB700 50μg; BB700.
Sundaram, H., Strange, Philip G. (1994) Characterisation of the human brain serotonin 5-HT1A receptor expressed in Chinese Hamster Ovary cells. Biochemical Society Transactions, 22 (1). S75-S75. ISSN 0300-5127. (doi:10.1042/bst022075s) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided) ...
Musser, G.G.; Carleton, M.D. (2005). "Subfamily Cricetinae". In Wilson, D.E.; Reeder, D.M. Mammal Species of the World: A ... Phodopus, a genus of rodents in the hamster subfamily Cricetinae-a division of the larger family Cricetidae-is a lineage of ... The genus Phodopus is one of three well-supported lineages in Cricetinae, the other two being the genus Mesocricetus and the ... Phodopus is sister to all other Cricetinae (meaning that it is the first lineage to diverge in the clade). Using several ...
"ADW: Cricetinae: INFORMATION". Animal Diversity Web. Retrieved 2012-11-03. Klabunde RE, Calvello C (November 1995). "Inhibition ... subfamily Cricetinae) is food hoarding. Hamsters carry food to their underground storage chambers using their spacious cheek ...
Two new genera of South American rodents (Cricetinae). Proceedings of the Biological Society of Washington 72:5-10. Hershkovitz ...
access-date= requires ,url= (help) Vorontsov, N.N (1960). "Species of Palaearctic hamsters (Cricetinae, Rodentia) in statu ... Cricetinae Murray, 1866) der Paläarctic". Zeitschrift für Säugetierkunde (in German). 20: 129-149. , ...
Supplementary notes on Neotropical Oryzomys dimidiatus and Oryzomys hammondi (Cricetinae). Journal of Mammalogy 51(4): 789-794 ...
A new rice rat of the Oryzomys palustris group (Cricetinae, Muridae) from northwestern Colombia, with remarks on distribution ( ... Supplementary notes on Neotropical Oryzomys dimidiatus and Oryzomys hammondi (Cricetinae) (subscription required). Journal of ...
A systematic review of the Neotropical water rats of the genus Nectomys (Cricetinae). Miscellaneous Publications, Museum of ... Supplementary notes on Neotropical Oryzomys dimidiatus and Oryzomys hammondi (Cricetinae) (subscription required). Journal of ...
Supplementary notes on Neotropical Oryzomys dimidiatus and Oryzomys hammondi (Cricetinae) (subscription required). Journal of ...
A systematic review of the Neotropical water rats of the genus Nectomys (Cricetinae). Miscellaneous Publications Museum of ...
Subfamily Cricetinae Genus Allocricetulus Species A. curtatus-Mongolian hamster Species A. eversmanni-Eversmann's or Kazakh ... Hamsters are rodents (order Rodentia) belonging to the subfamily Cricetinae, which contains about 25 species classified in six ... Some similar rodents sometimes called "hamsters" are not currently classified in the hamster subfamily Cricetinae. These ... Taxonomists generally disagree about the most appropriate placement of the subfamily Cricetinae within the superfamily Muroidea ...
Karyotypic variation in oryzomyine rodents (Cricetinae) with comments on chromosomal evolution in the Neotropical cricetine ...
The golden hamster, or Syrian hamster (Mesocricetus auratus) is a member of the subfamily Cricetinae, the hamsters. In the wild ...
... and a reappraisal of the dichotomy within New World Cricetinae". Miscellaneous Publications. Museum of Zoology, University of ...
... and a reappraisal of the dichotomy within New World Cricetinae. Miscellaneous Publications, Museum of Zoology, University of ...
... and a reappraisal of the dichotomy within New World Cricetinae. Cuartas-Calle, Carlos Auturo and Javier Muñoz-Arango. 2003. ...
A new rice rat of the Oryzomys palustris group (Cricetinae, Muridae) from northwestern Colombia, with remarks on distribution ( ...
Raquel López-Antoñanzas; Pablo Peláez-Campomanes; Ángeles Álvarez-Sierra (2014). "New Species of Rotundomys (Cricetinae) from ...
Hershkovitz, P. "South American swamp and fossorial rats of the scapteromyine group (Cricetinae, Muridae) with comments to the ...
The common hamster is a nocturnal or crepuscular species. It lives in a complex burrow system. It eats seeds, legumes, root vegetables, grasses and insects. It transports its food in its elastic cheek pouches to the food storage chambers. These may be quite large and may consist of a total of 65 kg of food.[9] It hibernates between October and March. During this time, it wakes every five to seven days to feed from the storage chambers. The adults reach sexual maturity when they are about 43 days old and breed from early April to August. The gestation period is 18-20 days and the size of the litter ranges from three to 15 young, which are weaned when aged three weeks. They are usually solitary animals.[9] ...
Subfamily: Cricetinae *Genus: Cricetulus *Grey dwarf hamster Cricetulus migratorius LR/nt. *Subfamily: Arvicolinae *Genus: ...
The golden hamster or Syrian hamster (Mesocricetus auratus) is a rodent in the subfamily Cricetinae, the hamsters.[2] Their ...
Cricetinae Genus: Cricetulus Grey dwarf hamster Cricetulus migratorius LR/nt Subfamily: Arvicolinae Genus: Microtus Günther's ...
Cricetinae. Genus: Phodopus. Miller, 1910. The dwarf hamsters represent a group of small hamsters in the genus Phodopus. ...
Cricetinae Genus: Cricetus European hamster Cricetus cricetus LR/lc Subfamily: Arvicolinae Genus: Ondatra Muskrat Ondatra ...
Cricetinae Genus: Cricetulus Grey dwarf hamster Cricetulus migratorius LR/nt Subfamily: Arvicolinae Genus: Clethrionomys Bank ...
Results for Cricetinae. Publications & Outputs. *. Time-resolved and two-photon emission imaging microscopy of live cells with ...
The common hamster is a nocturnal or crepuscular species. It lives in a complex burrow system. It eats seeds, legumes, root vegetables, grasses and insects. It transports its food in its elastic cheek pouches to the food storage chambers. These may be quite large and may consist of a total of 65 kg of food.[9] It hibernates between October and March. During this time, it wakes every five to seven days to feed from the storage chambers. The adults reach sexual maturity when they are about 43 days old and breed from early April to August. The gestation period is 18-20 days and the size of the litter ranges from three to 15 young, which are weaned when aged three weeks. They are usually solitary animals.[9] ...
Subfamily: Cricetinae *Genus: Cricetulus *Grey dwarf hamster Cricetulus migratorius LR/nt. *Subfamily: Arvicolinae *Genus: ...
Cricetinae • Genus: Phodopus • Species: Phodopus sungorus (Pallas, 1773) ...
Cricetinae; Cricetulus. Attributes. cell line. CHO-K1. ...
Cricetinae (1). Cricetulus griseus (1). Cryptococcus neoformans (1). Danio rerio (1). Debaryomyces hansenii (1). ...
Expression system: Cricetinae gen. sp.. UniProt: *Canonical: P02787 (Residues: 20-354; Coverage: 49%) ...
Cricetinae * Digestive System / chemistry * Duodenum / metabolism * Erythromycin / pharmacology * Gastrointestinal Agents / ...
Cricetinae * Cricetulus / genetics* * Humans * In Situ Hybridization, Fluorescence * Mice / genetics* * Physical Chromosome ...
Expression System: Cricetinae. *Deposited: 2013-04-01 Released: 2013-07-24 *Deposition Author(s): Wei, A., Anumula, R. ...
Expression System: Cricetinae. *Deposited: 2013-04-02 Released: 2013-08-21 *Deposition Author(s): Wei, A., Anumula, R. ...
Cricetinae. *Dose-Response Relationship, Drug. *Ear, Inner. *Ear, Inner: drug effects. *Ear, Inner: metabolism ...
Cricetinae. Genus: Phodopus. Miller, 1910. The dwarf hamsters represent a group of small hamsters in the genus Phodopus. ...
SUBFAMILY Cricetinae --, GENUS Phodopus SPECIES Phodopus sungorus ...
Musser, G.G.; Carleton, M.D. (2005). "Subfamily Cricetinae". In Wilson, D.E.; Reeder, D.M. Mammal Species of the World: A ... Phodopus, a genus of rodents in the hamster subfamily Cricetinae-a division of the larger family Cricetidae-is a lineage of ... The genus Phodopus is one of three well-supported lineages in Cricetinae, the other two being the genus Mesocricetus and the ... Phodopus is sister to all other Cricetinae (meaning that it is the first lineage to diverge in the clade). Using several ...
Comparative Immunology of Old World Hamsters -- Cricetinae John E. Coe. Pages 95-101 ...
"ADW: Cricetinae: INFORMATION". Animal Diversity Web. Retrieved 2012-11-03. Klabunde RE, Calvello C (November 1995). "Inhibition ... subfamily Cricetinae) is food hoarding. Hamsters carry food to their underground storage chambers using their spacious cheek ...
Cricetinae. Cricetulus. Liver / cytology, metabolism. Multiprotein Complexes / metabolism. Peroxisomes / metabolism*. Rats. ...
Cricetinae. Cricetulus. Databases, Protein. HeLa Cells. Humans. Lysine / metabolism. Nitrogen Isotopes. Nuclear Proteins / ...
Cricetinae; Cricetulus. OX NCBI_TaxID=10029 {ECO:0000313,EMBL:EGW00832.1, ECO:0000313,Proteomes:UP000001075}; RN [1] {ECO: ...
Cricetinae; Cricetulus. OX NCBI_TaxID=10029 {ECO:0000313,EMBL:EGW13235.1, ECO:0000313,Proteomes:UP000001075}; RN [1] {ECO: ...
Subfamilia: Cricetinae Genus: Mesocricetus Species: Mesocricetus auratus Name[edit]. Mesocricetus auratus (Waterhouse, 1839) ...
Subfamilia: Cricetinae Genus: Tscherskia. Species: T. triton Name[edit]. Tscherskia (Ognev, 1914) ...
  • Phodopus is sister to all other Cricetinae (meaning that it is the first lineage to diverge in the clade). (wikipedia.org)
  • Karyotypic variation in Oryzomine rodents (Cricetidae) with comments on chromosomal evolution in the Neotropical cricetinae complex. (thefreedictionary.com)