A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.
Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
A chromosomal disorder characterized by MENTAL RETARDATION, broad thumbs, webbing of fingers and toes, beaked nose, short upper lip, pouting lower lip, agenesis of corpus callosum, large foramen magnum, keloid formation, pulmonary stenosis, vertebral anomalies, chest wall anomalies, sleep apnea, and megacolon. The disease has an autosomal dominant pattern of inheritance and is associated with deletions of the short arm of chromosome 16 (16p13.3).
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
Formation of an acetyl derivative. (Stedman, 25th ed)
A nuclear receptor coactivator with specificity for ESTROGEN RECEPTORS; PROGESTERONE RECEPTORS; and THYROID HORMONE RECEPTORS. It contains a histone acetyltransferase activity that may play a role in the transcriptional activation of chromatin regions.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A nuclear receptor coactivator with specificity for ESTROGEN RECEPTORS and PROGESTERONE RECEPTORS. It contains a histone acetyltransferase activity that may play a role in CHROMATIN REMODELING during the process of nuclear receptor-induced transcription. The coactivator has been found at elevated levels in certain HORMONE-DEPENDENT NEOPLASMS such as those found in BREAST CANCER.
Established cell cultures that have the potential to propagate indefinitely.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.
Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
An activating transcription factor that regulates expression of a variety of genes including C-JUN GENES and TRANSFORMING GROWTH FACTOR BETA2.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. The tax (trans-activator x; x is undefined) proteins act by binding to enhancer elements in the LTR.
Activating transcription factors were originally identified as DNA-BINDING PROTEINS that interact with early promoters from ADENOVIRUSES. They are a family of basic leucine zipper transcription factors that bind to the consensus site TGACGTCA of the cyclic AMP response element, and are closely related to CYCLIC AMP-RESPONSIVE DNA-BINDING PROTEIN.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
Transport proteins that carry specific substances in the blood or across cell membranes.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.
A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).
A family of immunophilin proteins that bind to the immunosuppressive drugs TACROLIMUS (also known as FK506) and SIROLIMUS. EC 5.2.1.-
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A poly(A) binding protein that has a variety of functions such as mRNA stabilization and protection of RNA from nuclease activity. Although poly(A) binding protein I is considered a major cytoplasmic RNA-binding protein it is also found in the CELL NUCLEUS and may be involved in transport of mRNP particles.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Proteins transcribed from the E1A genome region of ADENOVIRUSES which are involved in positive regulation of transcription of the early genes of host infection.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A family of soluble proteins that bind insulin-like growth factors and modulate their biological actions at the cellular level. (Int J Gynaecol Obstet 1992;39(1):3-9)
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Intracellular proteins that reversibly bind hydrophobic ligands including: saturated and unsaturated FATTY ACIDS; EICOSANOIDS; and RETINOIDS. They are considered a highly conserved and ubiquitously expressed family of proteins that may play a role in the metabolism of LIPIDS.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A poly(A) binding protein that is involved in promoting the extension of the poly A tails of MRNA. The protein requires a minimum of ten ADENOSINE nucleotides in order for binding to mRNA. Once bound it works in conjunction with CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR to stimulate the rate of poly A synthesis by POLY A POLYMERASE. Once poly-A tails reach around 250 nucleotides in length poly(A) binding protein II no longer stimulates POLYADENYLATION. Mutations within a GCG repeat region in the gene for poly(A) binding protein II have been shown to cause the disease MUSCULAR DYSTROPHY, OCULOPHARYNGEAL.
One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
A 12-KDa tacrolimus binding protein that is found associated with and may modulate the function of calcium release channels. It is a peptidyl-prolyl cis/trans isomerase which is inhibited by both tacrolimus (commonly called FK506) and SIROLIMUS.
A family of secreted multidomain proteins that were originally identified by their association with the latent form of TRANSFORMING GROWTH FACTORS. They interact with a variety of EXTRACELLULAR MATRIX PROTEINS and may play a role in the regulation of TGB-beta bioavailability.
A cell line derived from cultured tumor cells.
One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.

The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus. (1/1067)

Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding protein (CBP) and p300. A stable complex containing both Zta and CBP could be isolated from lytically stimulated, but not latently infected RAJI nuclear extracts. Zta-mediated viral reactivation and transcriptional activation were both significantly inhibited by coexpression of the E1A 12S protein but not by an N-terminal deletion mutation of E1A (E1ADelta2-36), which fails to bind CBP. Zta bound directly to two related cysteine- and histidine-rich domains of CBP, referred to as C/H1 and C/H3. These domains both interacted specifically with the transcriptional activation domain of Zta in an electrophoretic mobility shift assay. Interestingly, we found that the C/H3 domain was a potent dominant negative inhibitor of Zta transcriptional activation function. In contrast, an amino-terminal fragment containing the C/H1 domain was sufficient for coactivation of Zta transcription and viral reactivation function. Thus, CBP can stimulate the transcription of latent EBV in a histone acetyltransferase-independent manner mediated by the CBP amino-terminal C/H1-containing domain. We propose that CBP may regulate aspects of EBV latency and reactivation by integrating cellular signals mediated by competitive interactions between C/H1, C/H3, and the Zta activation domain.  (+info)

The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (2/1067)

Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha-stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha-inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness.  (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (3/1067)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP.  (+info)

Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP. (4/1067)

Yeast two-hybrid system was employed to isolate novel proteins that physically interact with PU.1, a member of Ets family transcription factors. Sequence analyses of several isolated clones positive for beta-galactosidase activity revealed that one of these clones was confirmed to encode a transcriptional coactivator, CREB binding protein (CBP). GST binding assay showed that the interacting sites were located at the transcriptional activation domain of PU.1 through 74-122 and the region spanning residues 1283-1915 of CBP. CBP potentiated PU.1-mediated transcription of the reporter gene driven by the multimerized PU.1-binding sites, suggesting that CBP functions as a coactivator for PU.1. Considering that CBP is a limited cellular component to function as a coactivator for several transcription factors, CBP may mediate synergistic and antagonistic interactions between PU.1 and other transcription factors during the process of hematopoietic cell differentiation.  (+info)

c-Jun functions as a calcium-regulated transcriptional activator in the absence of JNK/SAPK1 activation. (5/1067)

Calcium is the principal second messenger in the control of gene expression by electrical activity in neurons. Recruitment of the coactivator CREB-binding protein, CBP, by the prototypical calcium-responsive transcription factor, CREB and stimulation of CBP activity by nuclear calcium signals is one mechanism through which calcium influx into excitable cells activates gene expression. Here we show that another CBP-interacting transcription factor, c-Jun, can mediate transcriptional activation upon activation of L-type voltage-gated calcium channels. Calcium-activated transcription mediated by c-Jun functions in the absence of stimulation of the c-Jun N-terminal protein kinase (JNK/SAPK1) signalling pathway and does not require c-Jun amino acid residues Ser63 and Ser73, the two major phosphorylation sites that regulate c-Jun activity in response to stress signals. Similar to CREB-mediated transcription, activation of c-Jun-mediated transcription by calcium signals requires calcium/ calmodulin-dependent protein kinases and is dependent on CBP function. These results identify c-Jun as a calcium-regulated transcriptional activator and suggest that control of coactivator function (i.e. recruitment of CBP and stimulation of CBP activity) is a general mechanism for gene regulation by calcium signals.  (+info)

Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in hormone signaling. (6/1067)

Disaggregation of the spherical nuclear bodies termed promyelocytic (PML) oncogenic domains (PODs) is a characteristic of acute promyelocytic leukemia. Here, we demonstrate that the cAMP enhancer binding protein (CREB)-binding protein (CBP) associates with PML in vitro and is recruited to the PODs in vivo. Through its association with CBP, wild-type PML dramatically stimulates nuclear receptor transcriptional activity. These results demonstrate that a fraction of CBP is compartmentalized to the POD through its association with PML and thus suggest that PML and other POD-associated proteins may play an unexpectedly broad role in aspects of transcriptional regulation and human disease.  (+info)

CREB binding protein coordinates the function of multiple transcription factors including nuclear factor I to regulate phosphoenolpyruvate carboxykinase (GTP) gene transcription. (7/1067)

Nuclear factor I (NFI) binds to a region of the phosphoenolpyruvate carboxykinase (GTP) (PEPCK) gene promoter adjacent to the cAMP regulatory element (CRE) and inhibits the induction of transcription from the gene promoter caused by the catalytic subunit of protein kinase A. In vivo footprinting studies demonstrated that both the CRE and the NFI-binding site are occupied by transcription factors, regardless of the presence of factors that stimulate (dibutyryl cAMP or dexamethasone) or inhibit (insulin) transcription from the PEPCK gene promoter. The NFI effects on transcription from the PEPCK gene promoter were observed even in the absence of the NFI binding site, suggesting the possibility of other weaker binding sites on the promoter or an interaction of NFI with a transcriptional co-activator. A mammalian two-hybrid system was used to demonstrate direct interaction between the transactivation domain of NFI-C and the CREB binding domain of the CREB-binding protein (CBP). Overexpression of a gene fragment encoding the CREB binding domain of CBP stimulates transcription from the PEPCK gene promoter. The inhibitory effect of NFI on transcription of the PEPCK gene induced by the catalytic subunit of protein kinase A appears to be the result of an interaction between NFI and the CREB-binding protein in which NFI competes with CREB for binding to the CREB-binding site on CBP. In contrast, glucocorticoids and thyroid hormone use the steroid hormone receptor binding domain of CBP to stimulate transcription from the PEPCK gene promoter. NFI-A combines with dexamethasone or thyroid hormone in an additive manner to stimulate PEPCK gene transcription. We conclude that CBP coordinates the action of the multiple factors known to control transcription of the PEPCK gene.  (+info)

CREB-binding protein is a transcriptional coactivator for hepatocyte nuclear factor-4 and enhances apolipoprotein gene expression. (8/1067)

Hepatocyte nuclear factor-4 (HNF-4) is a liver-enriched transcription factor that is crucial in the regulation of a large number of genes involved in glucose, cholesterol, and fatty acid metabolism and in determining the hepatic phenotype. We have previously shown that HNF-4 contains transcription activation functions at the N terminus (AF-1) and the C terminus (AF-2) which work synergistically to confer full HNF-4 activity. Here, we show that HNF-4 recruits the CREB-binding protein (CBP) coactivator on promoters of genes that contain functional HNF-4 sites. HNF-4 interacts with the N-terminal region of CBP (amino acids 1-771) and the C-terminal region of CBP (amino acids 1812-2441). The two activating functions of HNF-4, AF-1 and AF-2, interact with the N terminus and the N and C terminus of CBP, respectively. In addition, we show that in contrast to the other nuclear hormone receptors the interaction between HNF-4 and CBP is ligand-independent. Recruitment of CBP by HNF-4 results in an enhancement of the transcriptional activity of the latter. CBP does not activate gene expression in the absence of HNF-4, and dominant negative forms of HNF-4 prevent transcriptional activation by CBP, suggesting that the mere recruitment of CBP by HNF-4 is not sufficient for enhancement of gene expression. These findings demonstrate that CBP acts as a transcriptional coactivator for HNF-4 and provide new insights into the regulatory function of HNF-4.  (+info)

TY - JOUR. T1 - C/EBPβ activates E2F-regulated genes in vivo via recruitment of the coactivator CREB-binding protein/p300. AU - Wang, Haitao. AU - Larris, Brian. AU - Peiris, T. Harshani. AU - Zhang, Liping. AU - Le Lay, John. AU - Gao, Yan. AU - Greenbaum, Linda E.. PY - 2007/8/24. Y1 - 2007/8/24. N2 - The E2F transcription factors play an essential role in regulating the G1- to S-phase transition of the cell cycle. Previous studies have identified the importance of interactions between E2Fs and other transcription factors as a mechanism for transcriptional control of a subset of E2F regulated target genes. However, the mechanisms responsible for E2F target gene specificity remain incompletely understood. Here we report that in a mammalian in vivo model of synchronized proliferation, C/EBPβ occupancy on the promoters of E2F-regulated growth-related genes increases as a function of cell cycle progression. C/EPBβ binding to these promoters is associated with recruitment of the coactivator ...
Histone lysine acetylation is central to epigenetic control of gene transcription. Bromodomains of chromosomal proteins function as acetyl-lysine (Kac) binding domains. However, how bromodomains recognize site-specific histones remains unanswered. Here, we report three three-dimensional solution structures of the bromodomains of the human transcriptional coactivators CREB-binding protein (CBP) and p300/CBP-associated factor (PCAF) bound to peptides derived from histone acetylation sites at lysines 36 and 9 in H3, and lysine 20 in H4. From structural and biochemical binding analyses, we determine consensus histone recognition by the bromodomains of PCAF and CBP, which represent two different subgroups of the bromodomain family. Through bromodomain residues in the ZA and BC loops, PCAF prefers acetylation sites with a hydrophobic residue at (Kac+2) position and a positively charged or aromatic residue at (Kac+3), whereas CBP favors bulky hydrophobic residues at (Kac+1) and (Kac+2), a positively ...
Members of the CREB-binding protein/p300-interacting transactivator with ED-rich tail (CITED) family bind CREB-binding protein and p300 with high affinity and regulate gene transcription. Gene knockout studies indicate that CITED2 is required for neural crest and neural tube development and that it functions as a co-activator for transcription factor AP-2 (TFAP2). Here we describe human CITED4, a new member of this family, which is encoded by a single exon mapping to chromosome 1p34--1p35. CITED4 and p300/CREB-binding protein are present in endogenous naturally occurring complexes, indicating that they interact physiologically. The interaction occurs between the cysteine-histidine-rich domain 1 of p300 and the carboxyl terminus of CITED4. In keeping with this, CITED4 functions as a transactivator when artificially targeted to a promoter element. CITED4 physically interacts with all TFAP2 isoforms in vitro and strongly co-activates all TFAP2 isoforms in Hep3B cells. Co-activation of TFAP2 requires amino
Mier1 encodes a novel transcriptional regulator and was originally isolated as a fibroblast growth factor early response gene. Two major protein isoforms have been identified, MIER1α and β, which differ in their C-terminal sequence. Previously, we demonstrated that both isoforms recruit histone deacetylase 1 (HDAC1) to repress transcription. To further explore the role of MIER1 in chromatin remodeling, we investigated the functional interaction of MIER1 with the histone acetyltransferase (HAT), Creb-binding protein (CBP). Using GST pull-down assays, we demonstrate that MIER1 interacts with CBP and that this interaction involves the N-terminal half (amino acids 1-283) of MIER1, which includes the acidic activation and ELM2 domains and the C-terminal half (amino acids 1094-2441) of CBP, which includes the bromo-, HAT, C/H3 and glutamine-rich domains. Functional analysis, using HEK293 cells, shows that the CBP bound to MIER1 in vivo has no detectable HAT activity. Histone 4 peptide binding assays
Avots, A., Buttmann, M., Chupvilo, S., Escher, C., Smola, U., Bannister, A.J., Rapp, U.R., Kouzarides, T., Serfling, E. CBP/p300 integrates Raf/Rac-signalling pathways in the transcriptional induction of NF-Atc during T cell activation Immunity 10: 515-524 (1999) Chirmule, N., Avots, A., LakshmiTamma, S.M., Pahwa, S., Serfling, E. CD4-mediated signals induce T cell dysfunction in vivo J. Immunol. 163: 644-649 (1999) Bannert, N., Avots, A., Baier, M., Serfling, E., Kurth, R. GA-binding protein factors, in concert with the coactivator CREB binding protein/p300, control the induction of the interleukin 16 promoter in T lymphocytes Proc. Natl. Acad. Sci. USA 96: 1541-1546 (1999) Chupvilo, S., Zimmer, M., Kerstan, A., Glöckner, J., Avots, A., Escher, C., Fischer, C., Inashkina, I., Jankevics, E., Berberich-Siebelt, F., Schmitt, E., Serfling, E. Alternative polyadenylation events contribute to the induction of NF-ATc in effector T cells Immunity 10: 261-269 (1999) Chupvilo, S., Avots, A., ...
Following the publication of this article [1], the authors found that the primers listed for CREB-binding protein were not correct. This mistake occurred during assembly of the primer table and the authors apologize for this error. This correction does not change the data included in the paper, their interpretation nor the conclusions drawn.
In the present study, we sought to examine the molecular basis for the differential regulation of several members of the IFN-α/β gene family (IFNA and IFNB) by IRF-3 and IRF-7. The IFNB, IFNA1, IFNA2, and RANTES promoters were activated by coexpression of either IRF-3 or IRF-7, whereas the IFNA4, IFNA7, and IFNA14 promoters were exclusively activated by IRF-7 and not by IRF-3. Analysis of protein-DNA interactions revealed that recombinant IRF-3 and IRF-7 selectively bound to different regions of the IFNB promoter; IRF-3 bound preferentially to the PRDIII domain of the IFNB promoter, while IRF-7 interacted exclusively with the PRDI domain. PCR-mediated DNA binding site selection results demonstrated that IRF-3 recognized the IRF consensus element 5′-GAAANNGAAANN-3′. Replacement of a single nucleotide within the GAAA core half-site was sufficient to preclude IRF-3 DNA binding. IRF-7 bound to a related sequence motif but with greater flexibility than IRF-3; a single nucleotide replacement did ...
The major finding in this study is that ethanol induces an increase in gene expression via CREB and PKA. This increase in gene expression requires both PKA and CREB phosphorylation. Although we had previously shown that exposure to ethanol resulted in phosphorylation of CREB in NG108-15 cells, there is accumulating evidence that CREB phosphorylation is not sufficient to regulate gene expression under the control of CREs; activation of the coactivator CREB-binding protein (CBP) and other downstream elements is also required for increases in CRE-mediated gene expression (Montminy, 1997; Cardinaux et al., 2000).Thibault et al. (2000) have reported increases in genes the expression of which is known to be cAMP-dependent. However, ethanol activates many different signal transduction pathways in addition to PKA (Diamond and Gordon, 1997), and most genes have regulatory elements activated or inhibited by all of these pathways. Therefore, the experiments presented here are the first demonstration that ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
We also aim to understand the structure-function relationship of very large (oversized) proteins, practically neglected from a structural point of view thus far. Structural biology has traditionally addressed the structure of small folded proteins, whereas the field of structural disorder has focused on either fully disordered proteins/regions or short disordered elements that undergo induced folding in the presence of their partner. Here we would like to probe into the structure of the very large transcriptional co-activator CREB-binding protein (CBP), by addressing its structure by means traditionally applied in the case of protein complexes. CBP has about seven domains and disordered linker regions connecting them, the topology of which will be outlined by a combination of high-resolution (NMR, X-ray) and low-resolution (MS, EM, AFM) techniques ...
Cancer, Epidermal Growth Factor, Gene, Growth, Survival, Cell Line, Tumors, Disease, Kinase, Mutation, Patient, Phenotype, Cell, Insulin, Mouse, Acetylation, Binding Protein, Creb-binding Protein, Neuroblastoma, Nuclear Export
Function: Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase ...
It does not matter how the diet is restricted. It can be in terms of carbohydrates,fats or proetins. What matters is the diet should be of low calorie. This brings about a change in the CBP proteins that control the genes related to cellular functions ...
The inappropriate sustained SNA increase in OSA patients likely contributes to hypertension, organ damage, and mortality; however, it is unclear how excessive SNA develops in these patients. Several factors, including obesity and increased carotid body chemoreceptor sensitivity due to intermittent hypoxia, have been considered. Obesity could mechanically obstruct the airway and increase SNA through leptin, insulin, angiotensin, and cytokine actions; however, many OSA patients are not obese (23). Carotid body hypersensitivity as a result of intermittent hypoxia has been confirmed in animal models of OSA. In fact, plasticity of the carotid body glomus cells with long-term sensory facilitation and sensitization have been reported (18, 24) and associated with ROS and NOX2-dependent accumulation of HIF1 and the transcriptional coactivator CREB-binding protein (25). Central neuroplasticity. A provocative possibility for OSA-associated SNA dysfunction is that excessive activation of CNS nuclei induces ...
Rubinstein-Taybi syndrome (RSTS) is an uncommon genetic disorder characterised by a typical facies, small stature, broad angulated thumbs and intellectual impairment. Dental changes are a minor, yet significant component of the condition. Craniofacial growth retardation in RSTS is frequently complicated by unerupted teeth, while dental caries is related to the inherent intellectual deficit. Dental problems necessitate interdisciplinary management in terms of oral surgery, conservative dentistry, periodontics and orthodontics. When affected individuals are unco-operative, certain dental procedures may warrant general anaesthesia. In these instances, dental and medical staff will combine their expertise to enhance the well-being of the patient. In addition, specific dental changes may alert the medical practitioner to the possible diagnosis of RSTS. In this article we document the oro-dental manifestations and review the oro-dental approach in the management of three patients with RSTS. Our experience in
Rubinstein, J. Broad thumb-hallux (Rubinstein-Taybi) Syndrome 1957-1988. Am J Med Gen Suppl . vol. 6. 1990. pp. 3-16. (An early review of 571 cases, this article provides a detailed description of the physical findings in this syndrome.). Wiley, S, Swayne, S, Rubinstein, J, Lanphear, N, Stevens, C. Rubinstein-Taybi syndrome medical guidelines. Am J Med Genet. vol. 119A. 2003. pp. 101-110. (This article includes specific surveillance and intervention recommendations compiled by a group of pediatric experts.). Cantani, A, Gagliesi, D. Rubinstein-Taybi syndrome. Review of 732 cases and analysis of typical traits. Eur Rev Med Pharmacol Sci. vol. 2. 1998. pp. 81-87. (This is an analysis of 732 cases and provides a summary of the physical findings of the syndrome and discusses epidemiology and genetics known at the time of publication.). Roelfsema, J, Peters, D. Rubinstein-Taybi syndrome: clinical and molecular overview. Expert Rev Mol Med. vol. 9. 2007. pp. 1-15. (This article details the ...
EP300-related Rubinstein-Taybi syndrome: Highlighted rare phenotypic findings and a genotype-phenotype meta-analysis of 74 patients.
Mutations in the coactivator CREB-binding protein (CBP) are a major cause of the human skeletal dysplasia Rubinstein-Taybi syndrome (RTS); however, the mechanism by which these mutations affect skeletal mineralization and patterning is unknown. Here, we report the identification of 3-phosphoinositide-dependent kinase 1 (PDK1) as a key regulator of CBP activity and demonstrate that its functions map to both osteoprogenitor cells and mature osteoblasts. In osteoblasts, PDK1 activated the CREB/CBP complex, which in turn controlled runt-related transcription factor 2 (RUNX2) activation and expression of bone morphogenetic protein 2 (BMP2). These pathways also operated in vivo, as evidenced by recapitulation of RTS spectrum phenotypes with osteoblast-specific Pdk1 deletion in mice (Pdk1osx mice) and by the genetic interactions observed in mice heterozygous for both osteoblast-specific Pdk1 deletion and either Runx2 or Creb deletion. Finally, treatment of Pdk1osx and Cbp+/- embryos with BMPs in utero ...
This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in ...
hypothetical protein, A306_06942, Anapl_13162, AS27_07110, CBP, CBP/p300, CREB-binding protein, CREB binding protein (Rubinstein-Taybi syndrome), crebbp-a, crebbp-b, D623_10028045, E1A binding protein p300, EP300, H920_13788, hmm291030, KAT3A, M91_18874, MDA_GLEAN10009599, N301_13283, N302_12939, N303_04372, N307_13277, N308_10632, N309_02966, N311_11763, N312_01973, N321_00697, N326_12400, N327_01513, N332_08465, N334_05471, N335_14336, N336_02992, N339_02947, p300, p300/CBP, PAL_GLEAN10011621, RSTS, RTS, UY3_13419, Y1Q_016907, Z169_09090, crebbp ...
TY - JOUR. T1 - Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15. AU - Xie, Fuchun. AU - Fan, Qiuhua. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. PY - 2019/1/1. Y1 - 2019/1/1. N2 - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, we designed an ester prodrug of 666-15 through a long-range O,N-acyl transfer reaction for improved aqueous solubility. Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB.. AB - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, ...
Hop on to get the meaning of p/ CIP acronym / slang / Abbreviation. The Medical & Science Acronym / Slang p/ CIP means... AcronymsAndSlang. The p/ CIP acronym/abbreviation definition. The p/ CIP meaning is p300/ CREB-binding protein (CBP)-interacting protein. The definition of p/ CIP by AcronymAndSlang.com
TY - JOUR. T1 - Nuclear factor-kappaB and cAMP response element binding protein mediate opposite transcriptional effects on the Flk-1/KDR gene promoter.. AU - Illi, B.. AU - Puri, P.. AU - Morgante, L.. AU - Capogrossi, M. C.. AU - Gaetano, C.. PY - 2000/6/23. Y1 - 2000/6/23. N2 - -The vascular endothelial growth factor receptor Flk-1/KDR is highly expressed during development and almost disappears in adult tissues. Despite its biological relevance, little is known about the molecular mechanisms controlling its expression. In the present work, it is shown that cAMP response element binding protein (CREB) and nuclear factor-kappaB (NF-kappaB)-related antigens bind specific sequences in the Flk-1/KDR promoter. Functional studies demonstrate that cAMP represses whereas tumor necrosis factor-alpha, an activator of NF-kappaB, stimulates promoter activity. Histone acetyltransferases (HATs) P/CAF and CBP/p300 together with p65/RelA, the catalytic subunit of NF-kappaB, increase Flk-1/KDR promoter ...
CREBBP is targeted by inactivating mutations in follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL). Here, we provide evidence from transgenic mouse models that Crebbp deletion results in deficits in B-cell development and can cooperate with Bcl2 overexpression to promote B-cell lymphoma. Through transcriptional and epigenetic profiling of these B cells, we found that Crebbp inactivation was associated with broad transcriptional alterations, but no changes in the patterns of histone acetylation at the proximal regulatory regions of these genes. However, B cells with Crebbp inactivation showed high expression of Myc and patterns of altered histone acetylation that were localized to intragenic regions, enriched for Myc DNA binding motifs, and showed Myc binding. Through the analysis of CREBBP mutations from a large cohort of primary human FL and DLBCL, we show a significant difference in the spectrum of CREBBP mutations in these 2 diseases, with higher frequencies of ...
The CREBBP gene is associated with autosomal dominant Rubinstein-Taybi syndrome 1 (RSTS1) (MedGen UID: 48517) and is commonly deleted in the recurrent 16p13.3 microdeletion syndrome (OMIM: 610543), a severe form of RSTS resulting from a contiguous gene deletion involving CREBBP as well as other neighboring genes.
In 1963, Rubinstein and Taybi first described a malformation syndrome characterized by distinctive facies, mental retardation, broad thumbs, and broad great toes as are seen in the images below. {file44122}{file44123}{file44124}Deletions in band 16p13 have been described in association with this disorder, and mutations in the cyclic adenosin...
CREBBP - CREBBP (Myc-DDK-tagged)-Human CREB binding protein (CREBBP), transcript variant 1 available for purchase from OriGene - Your Gene Company.
Sigma-Aldrich offers abstracts and full-text articles by [Q Li, H Peng, H Fan, X Zou, Q Liu, Y Zhang, H Xu, Y Chu, C Wang, K Ayyanathan, F J Rauscher, K Zhang, Z Hou].
The concentration of glucose in the bloodstream is regulated by glucose itself, along with the hormones insulin and glucagon. Glucagon stimulates gluconeogenesis in part by regulating phosphorylation of a transcriptional coactivator known as cyclic adenosine monophosphate response element-binding protein 2 (CRTC2). Dentin et al. (see the Perspective by Birnbaum) found that high concentrations of circulating glucose also regulate CRTC2, but do so through stimulation of the hexosamine biosynthetic pathway and consequent O-linked glycosylation of the same serine residue in CRTC2 that is modified by phosphorylation. Thus, CRTC2 integrates signals from hormones and nutrients and might be a target for efforts to treat abnormalities of glucose homeostasis that are associated with diabetes.. R. Dentin, S. Hedrick, J. Xie, J. Yates, III, M. Montminy, Hepatic glucose sensing via the CREB coactivator CRTC2. Science 319, 1402-1405 (2008). [Abstract] [Full Text]. M. J. Birnbaum, Sweet conundrum. Science 319, ...
Total cell extracts were prepared using a high-salt detergent buffer (20 mm Hepes, pH 7.9, 350 mm NaCl, 20%[vol/vol] glycerol, 1%[wt/vol] NP-40, 1 mm MgCl2, 0.5 mm EDTA, 0.1 mm EGTA, 0.5 mm dithiothreitol, 0.1% PMSF, 1% aprotinin). Cells were harvested by centrifugation, washed once in ice-cold phosphate-buffered saline, and resuspended in four cell volumes of the detergent buffer. The cell lysate was incubated for 30 min on ice and then centrifuged for 5 min at 13,000 g at 4°C. EMSAs were performed with 32P-labeled oligonucleotides of either the inducible NF-κB or the constitutively active cyclic adenosine monophosphate response element binding protein (CREB) as previously described. 20The reaction mixture consisted of 37 μl purified water, 1 μl NF-κB or CREB oligonucleotides (25 ng/μl; Promega, Madison, WI), 5 μl kinase buffer, 5 μl γ-32P-dATP (Amersham International, Braunschweig, Germany), and 1.5 μl T4 kinase (polynucleotide kinase [PNK] buffer and PNK T4 kinase; New England ...
Roelfsema, J. H., White, S. J., Ariyürek, Y., Bartholdi, D., Niedrist, D., Papadia, F., … Peters, D. J. M. (2005). Genetic Heterogeneity in Rubinstein-Taybi Syndrome: Mutations in Both the CBP and EP300 Genes Cause Disease. The American Journal of Human Genetics, 76(4), 572-580. DOI:10.1086/429130 PMID:15706485 ...
Lysine propionylation and butyrylation are protein modifications that were recently identified in histones. The molecular components involved in the two protein modification pathways are unknown, hindering further functional studies. Here we report identification of the first three in vivo non-histone protein substrates of lysine propionylation in eukaryotic cells: p53, p300, and CREB-binding protein. We used mass spectrometry to map lysine propionylation sites within these three proteins. We also identified the first two in vivo eukaryotic lysine propionyltransferases, p300 and CREB-binding protein, and the first eukaryotic depropionylase, Sirt1. p300 was able to perform autopropionylation on lysine residues in cells. Our results suggest that lysine propionylation, like lysine acetylation, is a dynamic and regulatory post-translational modification. Based on these observations, it appears that some enzymes are common to the lysine propionylation and lysine acetylation regulatory pathways. Our ...
In type 2 diabetes, chronic hyperglycemia is detrimental to beta-cells, causing apoptosis and impaired insulin secretion. The transcription factor cAMP-responsive element-binding protein (CREB) is crucial for beta-cell survival and function. We inves
TY - JOUR. T1 - Design, synthesis and biological evaluation of regioisomers of 666-15 as inhibitors of CREB-mediated gene transcription. AU - Xie, Fuchun. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. N1 - Funding Information: This work was made possible through financial supports provided by the United States National Institutes of Health ( R01GM087305 ) and OHSU Office of Technology Transfer and Business Development . PY - 2017/2/1. Y1 - 2017/2/1. N2 - cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the pathogenesis and maintenance of various types of human cancers. Identification of small molecule inhibitors of CREB-mediated gene transcription has been pursued as a novel strategy for developing cancer therapeutics. We recently discovered a potent and cell-permeable CREB inhibitor called 666-15. 666-15 is a bisnaphthamide and has been shown to possess efficacious anti-breast cancer activity without toxicity in vivo. In this study, we ...
TY - JOUR. T1 - Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB. AU - Asahara, H.. AU - Santoso, B.. AU - Guzman, E.. AU - Du, K.. AU - Cole, P. A.. AU - Davidson, I.. AU - Montminy, M.. PY - 2001/11/22. Y1 - 2001/11/22. N2 - The cyclic AMP (cAMP)-responsive factor CREB induces target gene expression via constitutive (Q2) and inducible (KID, for kinase-inducible domain) activation domains that function synergistically in response to cellular signals. KID stimulates transcription via a phospho (Ser133)-dependent interaction with the coactivator paralogs CREB binding protein and p300, whereas Q2 recruits the TFIID complex via a direct association with hTAFII130. Here we investigate the mechanism underlying cooperativity between the Q2 domain and KID in CREB by in vitro transcription assay with naked DNA and chromatin templates containing the cAMP-responsive somatostatin promoter. The Q2 domain was highly active on a naked DNA template, and Ser133 ...
Extensive evidence implicates CREB-dependent gene transcription in memory (Bourtchuladze et al., 1994; Yin et al., 1994; Guzowski and McGaugh, 1997; Bartsch et al., 1998; Kida et al., 2002; Pittenger et al., 2002; Frankland et al., 2004). Multiple signaling pathways phosphorylate CREB at Ser133 (Shaywitz and Greenberg, 1999; Mayr and Montminy, 2001; Lonze and Ginty, 2002), which stimulates the recruitment of coactivators CBP/p300 (Chrivia et al., 1993; Parker et al., 1996). However, this phosphorylation event is not always sufficient to activate transcription (Impey et al., 1996; Mayr and Montminy, 2001) suggesting that CREB-mediated transcription is regulated by additional mechanisms.. In 2003, two laboratories identified a new family of CREB-specific coactivators, now referred to as CRTCs (Iourgenko et al., 2003; Conkright et al., 2003b). CRTC is thought to enhance transcription by facilitating the interaction of CREB with the RNA polymerase II pre-initiation complex (Conkright et al., 2003b; ...
The debate about the presence and role of intermediates in the folding of proteins has been a critical issue, especially for fast folders. One of the classical methodologies to identify such metastable species is the burst-phase analysis, whereby the observed signal amplitude from stopped-flow traces is determined as a function of denaturant concentration. However, a complication may arise when folding is sufficiently fast to jeopardize the reliability of the stopped-flow technique. In this study, we reassessed the folding of the KIX domain from cAMP Response Element-Binding (CREB)-binding protein, which has been proposed to involve the formation of an intermediate that accumulates in the dead time of the stopped flow. By using an in-house-built capillary continuous flow with a 50-μs dead time, we demonstrate that this intermediate is not present; the problem arose because of the instrumental limitation of the standard stopped flow to assess very fast refolding rate constants (e.g., ≥ 500 s⁻¹).
ERES MI HÉROE. Eres mi Héroe es un grupo creado con la intención de ayudar a mejorar la calidad de vida de Iván (www.eresmiheroe.com). Iván nació con cataratas e hipotonía. Los médicos creen que tiene una enfermedad rara de origen genético llamada Síndrome de Rubinstein-Taybi. Iván tiene 18 dioptrías y un 72% de minusvalía. Entre otras cosas, Iván necesita recibir sesiones de fisioterapia y rehabilitación para ganar fuerza y movilidad. ¡Te necesitamos! ¿Nos ayudas?. Created on {2}.
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The AlphaLISA SureFire Ultra p-CREB (Ser133) assay kit (High Volume) is an immunoassay for quantitative detection of phospho-CREB in cellular lysates.
I remember the fateful day when I saw my first Kix Commercial with its slogan Kid Tested, Mother Approved. In my ten-year-old mind, Id finally found the loophole in the cruel system of parental cereal control. Somehow, my mom actually obeyed this mantra. Kix occasionally landed in our cabinets, a brightly colored beacon in a sea of white and brown boxes. Recently, I bought a box to see if they were still as great as I remembered.
Rubinstein-Taybi Syndrome is a rare condition affecting 1:125,000 children. It is associated with short broad radially deviated thumbs, secondary to a delta proximal phalanx of the thumb. We undertook a retrospective review of seven children (13 thumbs) with Rubinstein-Taybi syndrome whose thumbs were treated using a corrective osteotomy to the delta phalanx over a 13 year period. The types of osteotomy used in the series were reverse wedge osteotomy, opening wedge osteotomy and dome shaped osteotomy. The mean preoperative radial deviation of thumbs was 68 degrees (range 45-85 degrees ). At follow up five of the 13 thumbs demonstrated some residual radial deviation. All recurrences occurred in the dome shaped osteotomy group. Our data suggest that surgery is effective in correcting the deformity, but there is a risk of incomplete correction or recurrence. Despite the recurrence the mean postoperative deformity was significantly better than preoperatively and the majority of patients families
Looking for online definition of cAMP-responsive element-binding protein 3-like protein 4 in the Medical Dictionary? cAMP-responsive element-binding protein 3-like protein 4 explanation free. What is cAMP-responsive element-binding protein 3-like protein 4? Meaning of cAMP-responsive element-binding protein 3-like protein 4 medical term. What does cAMP-responsive element-binding protein 3-like protein 4 mean?
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A chromosomal disorder characterized by MENTAL RETARDATION, broad thumbs, webbing of fingers and toes, beaked nose, short upper lip, pouting lower lip, agenesis of corpus callosum, large foramen magnum, keloid formation, pulmonary stenosis, vertebral anomalies, chest wall anomalies, sleep apnea, and megacolon. The disease has an autosomal dominant pattern of inheritance and is associated with deletions of the short arm of chromosome 16 (16p13.3 ...
Most reported microdeletions of the CREB-binding protein (CBP) gene in the Rubinstein-Taybi syndrome (RTS) were detected by fluorescence in situ hybridization (FISH) with a single cosmid probe specific to the 3 region of the gene. In order to test the hypothesis that the rate of microdeletion-posit …
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
TY - JOUR. T1 - The transcriptional coactivator and acetyltransferase p300 in fibroblast biology and fibrosis. AU - Ghosh, Asish K.. AU - Varga, John. PY - 2007/12/1. Y1 - 2007/12/1. N2 - The transcriptional coactivator p300 is a ubiquitous nuclear phosphoprotein and transcriptional cofactor with intrinsic acetyltransferase activity. p300 controls the expression of numerous genes in cell-type and signal-specific manner, and plays a pivotal role in cellular proliferation, apoptosis, and embryogenesis. By catalyzing acetylation of histones and transcription factors, p300 plays a significant role in epigenetic regulation. Recent evidence suggests that abnormal p300 function is associated with deregulated target gene expression, and is implicated in inflammation, cancer, cardiac hypertrophy, and genetic disorders such as the Rubinstein-Taybi syndrome. The activity of p300 is regulated at multiple levels, including developmental stage-specific expression, post-translational modifications, subcellular ...
4-PPBP is a molecule which binds to sigma receptors.[1] 4-PPBP decreases neuronal nitric oxide synthase (nNOS) activity and ischemia-evoked nitric oxide (NO) production. 4-PPBP provides neuroprotection; this involves the prevention of ischemia-induced intracellular Ca2+dysregulation.[2]4-PPBP protects neurons using a mechanism that activates the transcription factor cyclic adenosine monophosphate response element-binding protein (CREB). Neuroprotection that is associated with 4-PPBP increases Bcl-2 expression; Bcl-2 expression is regulated by CREB. [3] ...
RefSeq Summary (NM_001675): This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011 ...
Incubation of 3T3-L1 preadipocytes with isobutylmethylxanthine (IBMX), dexamethasone, and insulin, alone or in combination, demonstrated that IBMX, which increased cAMP-response element-binding protein (CREB) phosphorylation, was the predominant regulator of Pde3b expression. Real time PCR and immunoblotting indicated that in 3T3-L1 preadipocytes, IBMX-stimulated induction of Pde3b mRNA and protein was markedly inhibited by dominant-negative CREB proteins. By transfecting preadipocytes, differentiating preadipocytes, and HEK293A cells with luciferase reporter vectors containing different fragments of the 5- flanking region of the Pde3b gene, we identified a distal promoter that contained canonical cis-acting cAMP-response elements (CRE) and a proximal, GC-rich promoter region, which contained atypical CRE. Mutation of the CRE sequences dramatically reduced distal promoter activity; H89 inhibited IBMX-stimulated CREB phosphorylation and proximal and distal promoter activities. Distal promoter ...
Chromosomal translocations that fuse the mixed lineage leukemia (MLL) gene with multiple partners typify acute leukemias of infancy as well as therapy-related leukemias. We utilized a conditional knockin strategy to bypass the embryonic lethality caused by MLL-CBP expression and to assess the immediate effects of induced MLL-CBP expression on hematopoiesis. Within days of activating MLL-CBP, the fusion protein selectively expanded granulocyte/macrophage progenitors (GMP) and enhanced their self-renewal/proliferation. MLL-CBP altered the gene expression program of GMP, upregulating a subset of genes including Hox a9. Inhibition of Hox a9 expression by RNA interference demonstrated that MLL-CBP required Hox a9 for its enhanced cell expansion. Following exposure to sublethal γ-irradiation or N-ethyl-N-nitrosourea (ENU), MLL-CBP mice developed myelomonocytic hyperplasia and progressed to fatal myeloproliferative disorders. These represented the spectrum of therapy-induced acute myelomonocytic ...
Phosphorylated-cyclic adenosine monophosphate response element-binding protein (Phospho-CREB) has an important role in the pathogenesis of myocardial ischemia. We isolated the iridoid glycoside cornin from the fruit of Verbena officinalis L, investigated its effects against myocardial ischemia and r …
Expression of CREBBP (CBP, KAT3A, RSTS, RTS) in cervix, uterine tissue. Antibody staining with HPA055861 and CAB004212 in immunohistochemistry.
The highly conserved, multifunctional Tudor-SN (also called SND1/p100) modulates gene expression by an assortment of processes in the nucleus and in the cytoplasm. The multifunctional properties of Tudor-SN can be attributed to its complex structure, consisting of multiple domains within the 4SN and Tsn modules (Gutierrez-Beltran et al., 2016). As a key component in RNA splicing, processing, and editing, the 4SN module has RNA-binding and RNA cleavage activities (Caudy et al., 2003; Scadden, 2005; Li et al., 2008). This modular region also exhibits unique protein recognition properties, as it interacts with several components of the basal transcription machinery, including ATP-dependent RNA helicase A (Välineva et al., 2006), Signal Transducer and Activator of Transcription6 (Yang et al., 2002), DNA-directed RNA polymerase II largest subunit, and CREB-binding protein (Välineva et al., 2005; Yang et al., 2007). A subsequent study also demonstrated that the SN-like domains bind to the 3′ UTR ...
Here, we present a structural and dynamic description of CBP-ID4 at atomic resolution. ID4 is the fourth intrinsically disordered linker of CREB-binding protein (CBP). In spite of the largely disordered nature of CBP-ID4, NMR chemical shifts and relaxation measurements show a significant degree of α-helix sampling in the protein regions encompassing residues 2-25 and 101-128 (1852-1875 and 1951-1978 in full-length CBP). Proline residues are uniformly distributed along the polypeptide, except for the two α-helical regions, indicating that they play an active role in modulating the structural features of this CBP fragment. The two helical regions are lacking known functional motifs, suggesting that they represent thus-far uncharacterized functional modules of CBP. This work provides insights into the functions of this protein linker that may exploit its plasticity to modulate the relative orientations of neighboring folded domains of CBP and fine-tune its interactions with a multitude of ...
Somatic mutations in CREBBP occur frequently in B-cell lymphoma. Here, we show that loss of CREBBP facilitates the development of germinal center (GC)-derived lymphomas in mice. In both human and murine lymphomas, CREBBP loss-of-function resulted in focal depletion of enhancer H3K27 acetylation and aberrant transcriptional silencing of genes that regulate B-cell signaling and immune responses, including class II MHC. Mechanistically, CREBBP-regulated enhancers are counter-regulated by the BCL6 transcriptional repressor in a complex with SMRT and HDAC3, which we found to bind extensively to MHC class II loci. HDAC3 loss-of-function rescued repression of these enhancers and corresponding genes, including MHC class II, and more profoundly suppressed CREBBP-mutant lymphomas in vitro and in vivo. Hence, CREBBP loss-of-function contributes to lymphomagenesis by enabling unopposed suppression of enhancers by BCL6/SMRT/HDAC3 complexes, suggesting HDAC3-targeted therapy as a precision approach for ...
LCLs from patients with various kinds of chromatin abnormalities were obtained: ICF problem, CLS, FSHD and RSTS. Two of the three RSTS products had confirmedmutations in CREB binding protein. Nuclear extracts from these LCLs were immunoblotted for ATM s1981. demonstrates that low irradiated LCLs fromICF people displayedmarkedly increased amounts Geneticin manufacturer of ATM s1981 that resembled irradiated normal cells. In contrast to the ICF LCLs, samples from two FSHD patients displayed low phosphorylation levels that resembled the non irradiated get a handle on samples N1 and N 3. An individual with CLS and samples from three RSTS people also exhibited low phosphorylation levels that were slightly greater than the get a handle on samples, an effect that was reproducible. LCLs from an ATM individual failed to show ATM s1981 despite IR, as previously reported. The strong ATM s1981 transmission in the ICF products caused us to further examine these LCLs. We first addressed whether ATM s1981 in ...
The activation of the transcription factor NF-kB is central to the control of the cellular response triggered by many stimuli. Once released from the inhibitory molecule IkB, NF-kB is translocated to the nucleus, and it has to be phosphorylated to activate transcription. In protein kinase C (PKC)-deficient cells, NF-kB is transcriptionally inactive and the phosphorylation of the RelA subunit in response to tumor necrosis factor (TNF-alpha) is severely impaired. In vitro assays showed that PKC directly phosphorylates RelA. Here we demonstrate that Ser311 accounts for PKC phosphorylation of RelA and that this site is phosphorylated in vivo in response to TNF-alpha. Also, an inactivating mutation of that residue severely impairs RelA transcriptional activity, blocks its anti-apoptotic function and abrogates the interaction of RelA with the co-activator CBP as well as its recruitment, and that of RNA polymerase II (Pol II) with the interleukin-6 (IL-6) promoter. The interaction of endogenous CBP ...
Freds Dērsts piedzima Džeksonvilā, Floridā, ASV. Fredu audzināja viņa māte Anita, jo viņa bioloģiskais tēvs aizgāja no ģimenes, kad Fredam bija tikai dažas nedēļas. Freds ar māti dzīvoja ļoti trūcīgi, viņiem nebija ne mājas, ne darba, ne naudas. Viņi dzīvoja baznīcas bēniņos, ēdot bērnu pārtiku, ko nesa draudzes locekļi. Kad Fredam bija divi gadi, Anita iepazinās ar policijas oficieri Bilu, ar kuru drīz vien viņa apprecējās.. Fredam jaunībā patika tā pati mūzika, kas viņa vecākiem, un viņš bieži ākstījās dejojot un iztēlojoties, ka uzstājas publikas priekšā. Kad Freds paaugās, viņš un viņa pusbrālis Korijs (Bila un Anitas dēls) kļuva par Kiss faniem.. Vēlāk Freda ģimene pārvācās no Džeksonvillas, Floridā, uz Gastoniju, Ziemeļkarolīnā, kur Dērsts mācījās Hunter Huss vidusskolā.[1] Šeit viņš sāk aizrauties ar hiphopu un izveido breika dejošanas grupu Reckless Crew. Viņš sāk interesēties par kultūru, kas ir ...
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Vislielāko vīrusu daudzumu inficētie pacienti izdala 2.-3. slimības dienā, t.i., šajā periodā viņi ir visinfekciozākie, tomēr nelielos, inficēšanai pietiekamos daudzumos vīrusa izdale var saglabāties līdz pat 1-2 nedēļām.. Attiecībā uz sabiedrībā valdošo uzskatu, ka gaisa recirkulācijas sistēmas, piemēram, lidmašīnās, paaugstina saaukstēšanās risku, ASV 2002. gadā veiktais pētījums šādu faktu nav apstiprinājis, tāpēc šobrīd jādomā, ka pārneses ceļam ar nemazgātām rokām ir vislielākā nozīme saaukstēšanās vīrusu izplatībā.. Nereti tiek diskutēts par to, cik bieži gada laikā slimot ar saaukstēšanos ir normāli. Vidēji pirmsskolas vecuma bērni slimo 6-8 (līdz pat 12) reizes gadā, katru reizi slimībai ilgstot aptuveni 14 dienas, savukārt vecāki bērni un pieaugušie vidēji slimo 2-4 reizes gadā, katru reizi simptomiem ilgstot 5-7 dienas.. Smēķētājiem gan simptomi parasti ilgst vairāk par nedēļu hroniska elpceļu ...
Gerritsen ME, Williams AJ, Neish AS, Moore S, Shi Y, Collins T (Apr 1997). "CREB-binding protein/p300 are transcriptional ... Aarnisalo P, Palvimo JJ, Jänne OA (Mar 1998). "CREB-binding protein in androgen receptor-mediated signaling". Proceedings of ... "Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with ... Parry GC, Mackman N (Dec 1997). "Role of cyclic AMP response element-binding protein in cyclic AMP inhibition of NF-kappaB- ...
... also called EP300 or E1A binding protein p300) CBP (also known as CREB-binding protein or CREBBP) Both p300 and CBP interact ... Vo N, Goodman RH (Apr 2001). "CREB-binding protein and p300 in transcriptional regulation". The Journal of Biological Chemistry ... "Global transcriptional coactivators CREB-binding protein and p300 are highly essential collectively but not individually in ... "Extensive brain hemorrhage and embryonic lethality in a mouse null mutant of CREB-binding protein". Mechanisms of Development. ...
CREB-binding protein, Cyclin D1, Cyclin-dependent kinase 7, DACH1, Death associated protein 6, L-DOPA, EFCAB6, Epidermal growth ... Frønsdal K, Engedal N, Slagsvold T, Saatcioglu F (November 1998). "CREB binding protein is a coactivator for the androgen ... Aarnisalo P, Palvimo JJ, Jänne OA (March 1998). "CREB-binding protein in androgen receptor-mediated signaling". Proceedings of ... "The role of protein kinase A pathway and cAMP responsive element-binding protein in androgen receptor-mediated transcription at ...
... "p300/cAMP-response-element-binding-protein ('CREB')-binding protein (CBP) modulates co-operation between myocyte enhancer ... "cAMP-response-element-binding-protein-binding protein (CBP) and p300 are transcriptional co-activators of early growth response ... EP300 is closely related to another gene, CREB binding protein, which is found on human chromosome 16. p300 HAT functions as ... Chen Q, Dowhan DH, Liang D, Moore DD, Overbeek PA (July 2002). "CREB-binding protein/p300 co-activation of crystallin gene ...
... encodes CREB-binding protein which contributes to the activation of various genes); 3) TNFSF14 (encodes tumor necrosis factor ... "CREBBP CREB binding protein [Homo sapiens (human)] - Gene - NCBI". "TNFSF14 TNF superfamily member 14 [Homo sapiens (human)] - ... In consequence, CCND1 overexpresses cyclin D1, a protein which promotes the cell cycle and thereby cellular proliferation. The ... a kinase that regulates the activity of various tumor suppressors and cell cycle proteins). It may also involve gains in the ...
"MLL and CREB bind cooperatively to the nuclear coactivator CREB-binding protein". Molecular and Cellular Biology. 21 (7): 2249- ... and mutability at the binding interface between the p160 coactivator and CREB-binding protein". Protein Science. 13 (1): 203-10 ... "Cooperativity in transcription factor binding to the coactivator CREB-binding protein (CBP). The mixed lineage leukemia protein ... It was thus first termed CREB-binding domain. However, when it was later discovered that it also binds many other proteins, the ...
... has been shown to interact with CREB-binding protein. NFAT ENSG00000285485 GRCh38: Ensembl release 89: ENSG00000100968, ... NFATC4+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ... Nuclear factor of activated T-cells, cytoplasmic 4 is a protein that in humans is encoded by the NFATC4 gene. The product of ... Lahti AL, Manninen A, Saksela K (May 2003). "Regulation of T cell activation by HIV-1 accessory proteins: Vpr acts via distinct ...
2002). "CREB-binding protein/p300 co-activation of crystallin gene expression". J. Biol. Chem. 277 (27): 24081-9. doi:10.1074/ ... "CREB-binding protein/p300 co-activation of crystallin gene expression". J. Biol. Chem. United States. 277 (27): 24081-9. doi: ... Prospero homeobox protein 1 is a protein that in humans is encoded by the PROX1 gene. PROX1 has been shown to interact with ... PROX1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) Overview of all the structural ...
Hung HL, Kim AY, Hong W, Rakowski C, Blobel GA (Apr 2001). "Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)- ... Hung HL, Kim AY, Hong W, Rakowski C, Blobel GA (2001). "Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)-mediated ... NFE2 has been shown to interact with CREB-binding protein. GRCh38: Ensembl release 89: ENSG00000123405 - Ensembl, May 2017 ... and NF-E2-binding proteins". J. Biol. Chem. 277 (44): 41563-70. doi:10.1074/jbc.M208184200. PMID 12196550. Marini MG, Asunis I ...
Chen Q, Dowhan DH, Liang D, Moore DD, Overbeek PA (Jul 2002). "CREB-binding protein/p300 co-activation of crystallin gene ... Chen Q, Dowhan DH, Liang D, Moore DD, Overbeek PA (Jul 2002). "CREB-binding protein/p300 co-activation of crystallin gene ... MAF+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... Vanita V, Singh D, Robinson PN, Sperling K, Singh JR (Mar 2006). "A novel mutation in the DNA-binding domain of MAF at 16q23.1 ...
... has been shown to interact with CREB-binding protein. Biochemical pull down assays reveal SS18L1 to interact with ... SS18-like protein 1 is a protein that in humans is encoded by the SS18L1 gene. Synovial sarcomas occur most frequently in the ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode:2005Natur. ... X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 5 (3): ...
... has been shown to interact with CREB-binding protein. It was found that the transcription factor Klf4 present at the ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ...
The first AIRE partner that was identified is the CREB-binding protein (CBP) that is localized in nuclear bodies and is a co- ... Autoimmune regulator has been shown to interact with CREB binding protein. List of human clusters of differentiation for a list ... regulator protein has transcriptional transactivating properties and interacts with the common coactivator CREB-binding protein ... regulator protein has transcriptional transactivating properties and interacts with the common coactivator CREB-binding protein ...
... has been shown to interact with ZNF423 and CREB binding protein. GRCh38: Ensembl release 89: ENSG00000164330 - Ensembl, ... Transcription factor COE1 is a protein that in humans is encoded by the EBF1 gene. EBF1 stands for Early B-Cell Factor 1. EBF1 ... Tsai RY, Reed RR (Jun 1997). "Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 ... Tsai RY, Reed RR (1997). "Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 to ...
May 2009). "Metformin and insulin suppress hepatic gluconeogenesis through phosphorylation of CREB binding protein". Cell. 137 ... Transport of PEP across the mitochondrial membrane is accomplished by dedicated transport proteins; however no such proteins ... it triggers phosphorylation of enzymes and regulatory proteins by Protein Kinase A (a cyclic AMP regulated kinase) resulting in ... In the liver, the FOX protein FoxO normally promotes gluconeogenesis in the fasted state, but insulin blocks Fox0 upon feeding ...
Jin Y, Zeng SX, Dai MS, Yang XJ, Lu H (August 2002). "MDM2 inhibits PCAF (p300/CREB-binding protein-associated factor)-mediated ... The E3 ubiquitin ligase MDM2 is a negative regulator of the p53 tumor suppressor protein. MDM2 binds and ubiquitinates p53, ... HIPK2 is a protein that regulates Mdm2 in this way. The induction of the p14arf protein, the alternate reading frame product of ... Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by ...
"Transforming growth factor-beta regulates DNA binding activity of transcription factor Fli1 by p300/CREB-binding protein- ... "Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) ... Jin Y, Zeng SX, Dai MS, Yang XJ, Lu H (Aug 2002). "MDM2 inhibits PCAF (p300/CREB-binding protein-associated factor)-mediated ... The protein encoded by the PCAF gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, ...
... has been shown to interact with PBX1 and CREB-binding protein. Homeobox GRCh38: Ensembl release 89: ENSG00000260027 - ... "Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins". Genes Dev. 9 (6): 663-674. ... "Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins". Genes Dev. 9 (6): 663-674. ... This gene is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a ...
p90rsk also regulates transcription factors including cAMP response element-binding protein (CREB); estrogen receptor-α (ERα); ... Ribosomal+Protein+S6+Kinases,+90-kDa at the US National Library of Medicine Medical Subject Headings (MeSH) Ribosomal+Protein+ ... Erikson, Eleanor; Maller, James L. (1985). "A Protein Kinase from Xenopus Eggs Specific for Ribosomal Protein S6". PNAS. 82 (3 ... including other ribosomal proteins. Cytosolic substrates of p90rsk include protein phosphatase 1; glycogen synthase kinase 3 ( ...
"Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2" (PDF). Cellular Signalling. 27 (11): ... and protein molecules, and for protein engineering. Site-directed mutagenesis is one of the most important laboratory ... Commercial applications - Proteins may be engineered to produce mutant forms that are tailored for a specific application. For ... Site-directed mutagenesis is used to generate mutations that may produce a rationally designed protein that has improved or ...
"Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2" (PDF). Cell Signaling. 27 (11): 2252-60 ... See figure 3. of a recent study showing that CREB-binding protein is phosphorylated by HIPK2.) In plant physiology, ... Protein phosphorylation can be detected on an autoradiograph, after incubating the protein in vitro with the appropriate kinase ... The radiolabeled phosphate of latter is incorporated into the protein which is isolated via SDS-PAGE and visualized on an ...
... has been shown to interact with CREB-binding protein, MAPK1 and PEA15. GRCh38: Ensembl release 89: ENSG00000177189 - ... Du K, Montminy M (December 1998). "CREB is a regulatory target for the protein kinase Akt/PKB". The Journal of Biological ... Paudel HK (November 1997). "Phosphorylation by neuronal cdc2-like protein kinase promotes dimerization of Tau protein in vitro ... protein S6 kinase, 90kDa, polypeptide 3, also s RPS6KA3, is an enzyme that in humans is encoded by the RPS6KA3 gene. This gene ...
A possible role for interactions between Skn-1a and CREB-binding protein/p300". J. Biol. Chem. 276 (35): 33036-44. doi:10.1074/ ... POU domain, class 2, transcription factor 3 is a protein that in humans is encoded by the POU2F3 gene. GRCh38: Ensembl release ... 2001). "The POU domain factor Skin-1a represses the keratin 14 promoter independent of DNA binding. ...
Hung HL, Kim AY, Hong W, Rakowski C, Blobel GA (Apr 2001). "Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)- ... and Bach proteins (BACH1 and BACH2). sMaf homodimers bind to a palindromic DNA sequence called the Maf recognition element ( ... "A set of Hox proteins interact with the Maf oncoprotein to inhibit its DNA binding, transactivation, and transforming ... "Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells". Biophysical Journal. 83 (6): 3570-7. ...
"Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2" (PDF). Cellular Signalling. 27 (11): ... Green fluorescent protein (GFP) is also a photoprotein isolated and cloned from the jellyfish Aequorea victoria. Variants have ... activators or the different variants/fragments of the same activator are fused to the GAL4 DNA binding module at protein level ... This way the transcriptional activity of the different GAL4 fusion proteins can be directly compared using light as a readout. ...
... encodes CREB-binding protein which contributes to the activation of various genes); 3) TNFSF14 (encodes tumor necrosis factor ... Most such regimens add rituximab (a monoclonal antibody which binds and thereby kills the CD20 cell surface protein on B cells ... None of these protein markers or genomic abnormalities are diagnostic for FL, e.g. the t(14:18)(q32:q21.3) translocation is ... Suspect mutations include those in the following genes: 1) EZH2 (encodes polycomb repressive complex 2 family protein which is ...
Shimazu T, Horinouchi S, Yoshida M (Feb 2007). "Multiple histone deacetylases and the CREB-binding protein regulate pre-mRNA 3 ... SIRT2 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin ... The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from ... Studies of this protein have often been divergent, highlighting the dependence of pleiotropic effects of SIRT2 on cellular ...
This dimer then recruits co-activator CREB-binding protein (CBP) and is further phosphorylated. Once phosphorylated, this CLK- ... proteins causes formation of the PER-TIM heterodimer which prevents the CLK-CYC heterodimer from binding to the E-boxes of per ... leading to the degradation of both proteins. Hypophosphorylated CLK then accumulates, binds to the E-boxes of per and tim and ... The binding of CLOCK-BMAL to an E-box promoter element activates transcription of clock genes such as per1, 2, and 3 and tim in ...
CREB (cAMP response element binding protein) has been implicated in the upregulation of HULC. HULC RNA inhibits miR-372 ... or RNA-binding proteins (RBPs). Similarly, competition by ceRNAs for RNA-binding proteins has also been reported in eukaryotic ... The CD44 3'UTR has been shown to regulate expression of the CD44 protein and cell cycle regulation protein, CDC42, by ... transcriptomic miRNA-binding sites and (2) the impact miRNA concentrations have on the number of binding sites that must be ...
... is a protein that in humans is encoded by the NEUROG1 gene. NEUROG1 has been shown to interact with CREB-binding ... protein and decapentaplegic homolog 1. GRCh38: Ensembl release 89: ENSG00000181965 - Ensembl, May 2017 GRCm38: Ensembl release ...
RNA polymerase II regulatory region sequence-specific DNA binding. • DNA binding. • sequence-specific DNA binding. • ... Homeobox protein Hox-D8 is a protein that in humans is encoded by the HOXD8 gene.[5][6][7] ... 1989). "Complementary homeo protein gradients in developing limb buds". Genes Dev. 3 (5): 641-50. doi:10.1101/gad.3.5.641. PMID ... HOXD8+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) ...
The protein RSK2 which is encoded by the RPS6KA3 gene is a kinase which phosphorylates some substrates like CREB and histone H3 ... Deficiencies of intracellular signaling peptides and proteins. GTP-binding protein regulators. GTPase-activating protein. * ... RSK2 phosphorylates cellular proteins (including histone H3, and CREB), which regulate eukaryotic gene expression. In ... Mutations in the RPS6KA3 disturb the function of the protein, but it is unclear how a lack of this protein causes the signs and ...
protein homodimerization activity. · sequence-specific DNA binding. · metal ion binding. · protein heterodimerization activity ... C/EBP(α、β、γ、δ、ε、ζ) · CREB(1、3、L1) · CREM · DBP · DDIT3 · GABPA · HLF · MAF(B、F、G、K) · NFE(2、L1、L2、L3) · NFIL3 · NRL · NRF(1、2、3 ... DNA binding. · chromatin binding. · double-stranded DNA binding. · sequence-specific DNA binding transcription factor activity ... Rb · RBL1(英语:Retinoblastoma-like protein 1) · RBL2(英语:Retinoblastoma-like protein 2) ...
receptor binding. • связывание с белками плазмы. • гомодимеризация белка. • cell adhesion molecule binding. • protein binding ... positive regulation of CREB transcription factor activity. • heterotypic cell-cell adhesion. • positive regulation of ... positive regulation of protein-glutamine gamma-glutamyltransferase activity. • regulation of neuroinflammatory response. • ... Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins ...
receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ... Tao X, Finkbeiner S, Arnold DB, Shaywitz AJ, Greenberg ME (April 1998). "Ca2+ influx regulates BDNF transcription by a CREB ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... positive regulation of receptor binding. • regulation of protein localization to cell surface. • regulation of receptor ...
Blackwood EM, Eisenman RN (1991). "Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex ... CREB (1, 3, L1) • CREM • DBP • DDIT3 • GABPA • HLF • MAF (B, F, G, K) • NFE (2, L1, L2) • NFIL3 • NRL • NRF1 • XBP1 ... "Control of c-myc mRNA half-life in vitro by a protein capable of binding to a coding region stability determinant". Genes Dev. ... 1988). "A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is ...
Rouaux C, Loeffler JP, Boutillier AL (September 2004). "Targeting CREB-binding protein (CBP) loss of function as a therapeutic ... "Sodium valproate exerts neuroprotective effects in vivo through CREB-binding protein-dependent mechanisms but does not improve ... The UBQLN2 gene encodes the protein ubiquilin 2 which is responsible for controlling the degradation of ubiquitinated proteins ... As that gene's name suggests, BACE1 is an enzymatic protein that cleaves the Amyloid Precursor Protein into the insoluble ...
One of the nuclear targets for PKA is the transcriptional control protein CREB (cAMP response element binding protein).[5] In ... Kandel identified CREB as being a protein involved in long-term memory storage. One result of CREB activation is an increase in ... Neurotransmitters, second messenger systems, protein kinases, ion channels, and transcription factors like CREB have been ... By 1980, collaboration with Paul Greengard resulted in demonstration that cAMP-dependent protein kinase, also known as protein ...
This has revealed a common fold with amino acid-binding bacterial proteins and with the glutamate-binding module of AMPA- ... Hardingham GE, Fukunaga Y, Bading H (May 2002). "Extrasynaptic NMDARs oppose synaptic NMDARs by triggering CREB shut-off and ... Activation of the receptor depends on glutamate binding, D-serine or glycine binding at its GluN1-linked binding site and AMPA ... The NMDA receptor is a glutamate and ion channel protein receptor that is activated when glycine and glutamate bind to it.[2] ...
sequence-specific DNA binding. • DNA binding. • transcription factor binding. • protein domain specific binding. • RNA ... HNF-3G is a member of the forkheadclass of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional ... 2003). "Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte ... transcription factor activity, sequence-specific DNA binding. • transcription regulatory region DNA binding. • ...
... towards the transcriptional control of AMPA receptors in longer-term memory through cAMP response element-binding protein (CREB ... All AMPARs contain PDZ-binding domains, but which PDZ domain they bind to differs. For example, GluA1 binds to SAP97 through ... "Synaptic anchorage of AMPA receptors by cadherins through neural plakophilin-related arm protein AMPA receptor-binding protein ... Of note, AMPARs cannot directly bind to the common synaptic protein PSD-95 owing to incompatible PDZ domains, although they do ...
Protein kodiran ovim genom je član NR1 potfamilije nuklearnih hormonskih receptora. To je DNA-vezujući protein koji se može ... CREB (1, 3, L1) • CREM • DBP • DDIT3 • GABPA • HLF • MAF (B, F, G, K) • NFE (2, L1, L2) • NFIL3 • NRL • NRF1 • XBP1 ... 2001). "X-ray structure of the orphan nuclear receptor RORbeta ligand-binding domain in the active conformation.". Embo J. 20 ( ... 2000). "Differential ligand-dependent protein-protein interactions between nuclear receptors and a neuronal-specific cofactor ...
... and it is the last protein to bind in the complex.. Histones tend to be positively charged proteins with N-terminal tails that ... CREB, c-Jun, C/EBPβ, NF-E2, SREBP, IRF2, Sp3, YY1, KLF13, EVI1, BCL6, HNF-4, ER81, and FOXO4 (AFX).[18] ... structural proteins, polyamines, and proteins involved in nuclear import.[3] Acetylation of these proteins can alter their ... Many MYST proteins also contain a cysteine-rich, zinc-binding domain within the HAT region in addition to an N-terminal ...
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that bind to the sterol regulatory element DNA ... Sterol regulatory element-binding transcription factor 1. X-ray crystallography of Sterol Regulatory Element Binding Protein 1A ... SCAP, in turn, can bind reversibly with another ER-resident membrane protein, INSIG. In the presence of sterols, which bind to ... proteins. However, in contrast to E-box-binding HLH proteins, an arginine residue is replaced with tyrosine making them capable ...
bile acid binding. • protein binding. • ligand-dependent nuclear receptor binding. • transcription factor activity, RNA ... RNA polymerase II distal enhancer sequence-specific DNA binding. • sequence-specific DNA binding. • DNA binding. • ... metal ion binding. • retinoid X receptor binding. • steroid hormone receptor activity. • transcriptional activator activity, ... chenodeoxycholic acid binding. • transcription regulatory region sequence-specific DNA binding. • RNA polymerase II regulatory ...
... using a mechanism that activates the transcription factor cyclic adenosine monophosphate response element-binding protein (CREB ... "Cyclic adenosine monophosphate response element-binding protein phosphorylation and neuroprotection by 4-phenyl-1-(4- ... 4-PPBP is a molecule which binds to sigma receptors.[1] 4-PPBP decreases neuronal nitric oxide synthase (nNOS) activity and ... Neuroprotection that is associated with 4-PPBP increases Bcl-2 expression; Bcl-2 expression is regulated by CREB. [3] ...
Mitchell PJ, Tjian R (1989). „Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins". Science ... CREB (1, 3, L1) • CREM • DBP • DDIT3 • GABPA • HLF • MAF (B, F, G, K) • NFE (2, L1, L2) • NFIL3 • NRL • NRF1 • XBP1 ... Lee TI, Young RA (2000). „Transcription of eukaryotic protein-coding genes". Annu. Rev. Genet. 34: 77-137. PMID 11092823. doi: ... U molekularnoj biologiji i genetici, transkripcioni faktor (za sekvencu-specifični DNK-vezujući faktor) je protein koji se ...
... p38 mitogen-activated protein kinases (p38 Mpk), and cAMP response element-binding protein (CREB) which when activated ... G protein linkage[2]. pathways[2] Prostaglandin DP1 receptor. DP1. PGD2,,PGE2,PGF2α,PGI2=TXA2[6]. relaxant. Gs alpha subunit. ... stimulates PLC, IP3, PKC, ERK, p38 Mpk, and CREB Prostaglandin EP2 receptor. EP2. PGE2,PGF2α=PGI2,PGD2=TXA2[9]. relaxant. Gs ... stimulates AC, PKA, PI3K, AKT, ERK, p38 Mpk, & CREB; raises cAMP Prostaglandin F2α receptor. FP. PGF2α,PGD2,PGE2,PGI2=TXA2[12] ...
Acetylation of interferon regulatory factor-7 by p300/CREB-binding protein (CBP)-associated factor (PCAF) impairs its DNA ... RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity. • DNA binding. ... Morin P, Bragança J, Bandu MT, Lin R, Hiscott J, Doly J, Civas A (2002). «Preferential binding sites for interferon regulatory ... does not require double-stranded RNA-dependent protein kinase R or Ikappa B kinase but is blocked by Vaccinia virus E3L protein ...
protein binding. • RNA polymerase II core promoter proximal region sequence-specific DNA binding. • chromatin binding. • ... DNA binding. • p53 binding. • DNA binding, bending. • RNA polymerase II transcription factor binding. • chromatin DNA binding. ... zinc ion binding. • metal ion binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. • RNA ... core promoter binding. • transcription regulatory region sequence-specific DNA binding. • sequence-specific DNA binding. • RNA ...
MYB factors represent a family of proteins that include the conserved MYB DNA-binding domain. Plants contain a MYB-protein ... "Molecular cloning reveals that the p160 Myb-binding protein is a novel, predominantly nucleolar protein which may play a role ... Myb proto-oncogene protein also known as transcriptional activator Myb is a protein that in humans is encoded by the MYB gene.[ ... The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C- ...
Adenoviral E1A-associated protein of 300kDa (p300) and the CREB-binding protein (CBP) make up the next family of HATs.[10] This ... HDACs 1 and 2 can also bind directly to DNA binding proteins such as Yin and Yang 1 (YY1), Rb binding protein 1 and Sp1.[5] ... For HDACs 4, 5 and 7, conserved binding domains have been discovered that bind for C-terminal binding protein (CtBP), myocyte ... p300/CREB-binding protein associated factor (PCAF), Elp3, HPA2 and HAT1.[10][11] Major features of the GNAT family include HAT ...
DNA binding. • sequence-specific DNA binding. • protein domain specific binding. • transcription factor activity, sequence- ... Forkhead box protein A2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are ... "ErbB3 binding protein 1 represses metastasis-promoting gene anterior gradient protein 2 in prostate cancer". Cancer Res. 70 (1 ... transcription factor binding. • RNA polymerase II core promoter proximal region sequence-specific DNA binding. • transcription ...
The cAMP response element-binding protein (CREB) is a transcription factor which is believed to be important in consolidating ... This protein is an autonomously active form of the enzyme protein kinase C (PKC), known as PKMζ. PKMζ maintains the activity- ... Yin, Jerry CP; Tully, Timothy (1996). "CREB and the formation of long-term memory". Current Opinion in Neurobiology. 6 (2): 264 ... Long-term memory, unlike short-term memory, is dependent upon the synthesis of new proteins.[35] This occurs within the ...
Calcium binds to proteins such as calmodulin (CaM) and an eye-specific protein kinase C (PKC) known as InaC. These proteins ... protein kinase A, and a transcription factor known as CREB. These molecules were shown to be also involved in synaptic ... InaD contains five binding domains called PDZ domain proteins, which specifically bind the C termini of target proteins. ... In addition, proteins called arrestins bind metarhodopsin and prevent it from activating more Gq. A sodium-calcium exchanger ...
In protein synthesis, PKA first directly activates CREB, which binds the cAMP response element, altering the transcription and ... The dimerization and docking (D/D) domain of the dimer binds to the A-kinase binding (AKB) domain of A-kinase anchor protein ( ... Protein Kinase A is directed to specific sub cellular locations after tethering to Protein kinase A anchoring proteins (AKAPs ... The mechanisms of further effects may be divided into direct protein phosphorylation and protein synthesis: *In direct protein ...
McKay DB, Steitz TA (1981). "Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed ... CREB (1, 3, L1) • CREM • DBP • DDIT3 • GABPA • HLF • MAF (B, F, G, K) • NFE (2, L1, L2) • NFIL3 • NRL • NRF1 • XBP1 ... Brennan RG, Matthews BW (1989). "The helix-turn-helix DNA binding motif.". J Biol Chem 264 (4): 1903-6. PMID 2644244. ... Pabo CO, Lewis M (1982). "The operator-binding domain of lambda repressor: structure and DNA recognition.". Nature 298 (5873): ...
protein binding. • neuropeptide hormone activity. • peptide hormone receptor binding. Cellular component. • extracellular ... The PKA then activates other intracellular signaling pathways like the phosphorylation of CREB and other transcriptional ... When VIP binds to VPAC2 receptors, a G-alpha-mediated signalling cascade is triggered. In a number of systems, VIP binding ... positive regulation of protein catabolic process. • regulation of protein localization. • antimicrobial humoral immune response ...
... binds and inhibits the CREB binding protein and p300 histone acetyltransferease activities on a repressed gene target, cyclin ... Nascent long ncRNAs have been shown to increase the activity of CREB binding protein, which in turn increases the transcription ... In the broad sense, this mechanism allows the cell to harness RNA-binding proteins, which make up one of the largest classes ... July 2008). "Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription". Nature. 454 (7200): ...
transcription factor binding. • activating transcription factor binding. • E-box binding. • protein binding. • protein ... DNA binding. • sequence-specific DNA binding. • RNA polymerase II regulatory region sequence-specific DNA binding. • protein ... transcription regulatory region DNA binding. Cellular component. • transcription factor complex. • protein complex. • nuclear ... Heart- and neural crest derivatives-expressed protein 2 is a protein that in humans is encoded by the HAND2 gene.[5][6] ...
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ...
CREB-binding protein, also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene. The CREB protein ... "Histone binding protein RbAp48 interacts with a complex of CREB binding protein and phosphorylated CREB". Molecular and ... CREB-binding protein has been shown to interact with: TF2, AR, AIRE, BRCA1, C-jun, CSK, Ccaat-enhancer-binding proteins, CDX2, ... First isolated as a nuclear protein [Ref] that binds to cAMP-response element-binding protein (CREB), this gene is now known to ...
CREB- binding protein;. CREB,. cAMP response element binding protein;. GST,. glutathione S-transferase;. CAT,. chloramphenicol ... CREB-binding protein (CBP) is a coactivator that interacts with the cAMP-response element binding protein (CREB), a process ... CREB-binding protein/p300 are transcriptional coactivators of p65. Mary E. Gerritsen, Amy J. Williams, Andrew S. Neish, Sarah ... CREB-binding protein/p300 are transcriptional coactivators of p65. Mary E. Gerritsen, Amy J. Williams, Andrew S. Neish, Sarah ...
Here we report that CREB phosphorylated by protein kinase A binds specifically to a nuclear protein of M(r) 265K which we term ... for CREB-binding protein). Fusion of a heterologous DNA-binding domain to the amino terminus of CBP enables the chimaeric ... Phosphorylated CREB binds specifically to the nuclear protein CBP.. Chrivia JC1, Kwok RP, Lamb N, Hagiwara M, Montminy MR, ... Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation ...
CREB-binding protein (CREBBP) and preeclampsia: a new promising target gene. Title: CREB-binding protein (CREBBP) and ... CREBBP CREB binding protein [Homo sapiens] CREBBP CREB binding protein [Homo sapiens]. Gene ID:1387 ... Creb_binding; Creb binding. cl02120. Location:1342 → 1562. HAT_KAT11; Histone acetylation protein. ... HAT_KAT11; Histone acetylation protein. pfam09030. Location:1996 → 2073. Creb_binding; Creb binding. ...
2003). Intrathecal injection of cAMP response element binding protein (CREB) antisense. By Wise Young in forum Neuropathic Pain ... CREB-Binding Protein This sounds promising for the future....Wonder if exercise builds it up?. https://www.eurekalert.org/pub_ ... TAR DNA-binding protein 43 in neurodegenerative disease.. By wildwilly in forum Neurodegeneration Research ... 2001). RNA-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its ... acetyl-CoA + L-lysyl-[protein] = CoA + H+ + N6-acetyl-L-lysyl-[protein] UniProt ... This protein in other organisms (by gene name): Q4LE28 - Homo sapiens 0 * Q6GQV9 - Mus musculus no matching PDB entries ...
Drosophila CREB binding protein (CBP), which is encoded by the nejire (nej) locus, belongs to the CBP/p300 family of proteins ( ... and Dach proteins mediated through CREB binding protein. Mol. Cell. Biol. 22: 6759-6766. ... CREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila. Justin P. Kumar, Tazeen Jamal, Alex ... CREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila. Justin P. Kumar, Tazeen Jamal, Alex ...
Biochemical and NMR Mapping of the Interface between CREB-binding Protein and Ligand Binding Domains of Nuclear Receptor: ... The binding protein of cAMP response element binding protein (CBP) appears to be a promiscuous coactivator for an increasing ... a Transcriptional Activator That Interacts with Multiple Domains of cAMP-responsive Element-binding Protein (CREB)-binding ... CREB Binding Protein Is a Coactivator for the Androgen Receptor and Mediates Cross-talk with AP-1 ...
Our results indicate that Nrf2 exploits the cooperative binding of two independent transactivation domains to CBP in the ... Neh4 and Neh5 both individually and cooperatively bind to CBP (CREB (cAMP Responsive Element Binding protein) Binding Protein ... Two domains of Nrf2 cooperatively bind CBP, a CREB binding protein, and synergistically activate transcription Genes Cells. ... Importantly, the CBP-binding activity of Nrf2 deletion mutants positively correlated with their transactivation activity. Neh5 ...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
The small-molecule ICG-001 binds cAMP-responsive element binding (CREB)-binding protein (CBP) to disrupt its interaction with β ... The CREB-Binding Protein Inhibitor ICG-001 Suppresses Pancreatic Cancer Growth. Michael D. Arensman, Donatello Telesca, Anna R. ... The CREB-Binding Protein Inhibitor ICG-001 Suppresses Pancreatic Cancer Growth. Michael D. Arensman, Donatello Telesca, Anna R. ... The CREB-Binding Protein Inhibitor ICG-001 Suppresses Pancreatic Cancer Growth. Michael D. Arensman, Donatello Telesca, Anna R. ...
... use a wide variety of protein-protein interactions to properly regulate transcription of target genes. In an attempt to ... identify novel PPAR-interacting proteins, a cDNA expression library was screened with bacteria … ... Identification of the CREB-binding protein/p300-interacting protein CITED2 as a peroxisome proliferator-activated receptor ... One of the genes identified as a PPARalpha-associated protein by interaction cloning was the CREB-binding protein/p300- ...
Gerritsen, M.E., Williams, A.J., Neish, A.S., Moore, S., Shi, Y. and Collins, T. (1997) CREBbinding protein/p300 are ... von Mikecz, A., Zhang, S., Montminy, M., Tan, E.M. and Hemmerich, P. (2000) CREBbinding protein (CBP)/p300 and RNA polymerase ... Prothymosin α interacts with the CREBbinding protein and potentiates transcription. Zoe Karetsou, Adroniki Kretsovali, Carol ... We report here that ProTα physically interacts with the CREBbinding protein (CBP), which is a versatile transcription co‐ ...
We have previously shown that the transcriptional cofactor CREB-binding protein (CBP) binds to the zinc finger domain of GATA-1 ... CREB-Binding Protein Acetylates Hematopoietic Transcription Factor GATA-1 at Functionally Important Sites. Hsiao-Ling Hung, ... 1996) CREB-binding protein activates transcription through multiple domains. J. Biol. Chem. 271:28138-28145. ... 1998) CREB-binding protein (CBP) cooperates with transcription factor GATA-1 and is required for erythroid differentiation. ...
The CREB-binding protein(CBP) is a transcriptional co-activators of various sequence-specific DNA-binding transcription factors ... Immunohistochemical Expression of p53 Protein and CREB-binding Protein in Polyps and Adenocarcinomas of Colon. ... RESULTS: 1. p53 protein expression was observed in 15% (9/60) of hyperplastic polyps, in 68.9% (124/180) of adenomatous polyps ... This article examined the expression levels of the p53 protein and CBP as well as their diagnostic value in a biopsy sample. ...
Normal P53 Function Requires An Association With The CREB Binding ... Are Conferred Primarily Through The Binding And Inactivation Of ... Acetylation, Animals, Antigens, Viral, Tumor, CREB-Binding Protein, Cell Line, Humans, Mice, Phosphorylation, Protein Binding, ... Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53.. Borger DR, DeCaprio JA ... Normal p53 function requires an association with the CREB binding protein (CBP)/p300 coactivators, and a ternary complex ...
... cAMP Response Element Binding Protein)(48H2), Artikelnummer: C7915-25.100 von United States Biological bei Biomol kaufen! ... Produktinformationen "Anti-CREB (cAMP Response Element Binding Protein)(48H2)" CREB is a bZIP transcription factor that ... While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a ... Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate ...
1998) cAMP-response-element-binding-protein-binding protein (CBP) and p300 are transcriptional co-activators of early growth ... we show that LPS-mediated TNF-α transcription is dependent upon CREB binding protein (CBP) and p300 coactivator proteins and, ... assembly of the LPS-stimulated TNF-α enhancer complex is dependent upon the coactivator proteins CREB binding protein and p300 ... 1993) Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature 365:855-859. ...
GST-Tat was also used in binding studies with other proteins, including RelA and Stat2. Neither of these proteins bound to GST- ... 1993) Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature 365:855-859. ... Interaction of Human Immunodeficiency Virus Type 1 Tat with the Transcriptional Coactivators p300 and CREB Binding Protein. ... Among the factors associated with the basal transcription complex, p300 and the related CREB binding protein (CBP) (2) have ...
... the authors found that the primers listed for CREB-binding protein were not correct. This mistake occurred during assembly of ... Multiple functions of CREB-binding protein during postembryonic development: identification of target genes. BMC Genomics. 2017 ... Roy, A., George, S. & Palli, S.R. Correction to: Multiple functions of CREB-binding protein during postembryonic development: ... Correction to: Multiple functions of CREB-binding protein during postembryonic development: identification of target genes. * ...
Estradiol Increases ER Activity In Part By Enhancing Interactions Between Its Carboxy-terminal, Ligand-binding ... alpha And ERbeta Are Transcription Factors That Can Be Activated By Both Ligand Binding And Growth Factor Signaling. ... cAMP response element binding protein (CREB) binding ... Read More. protein) families of coactivators. In the absence of ligand ... Binding Sites, CREB-Binding Protein, Cells, Cultured, Estradiol, Estrogen Antagonists, Estrogen Receptor alpha, Female, Gene ...
Here we report that CREB is present in the mitochondrial matrix of neurons and that it binds directly to cyclic AMP response ... underlies the protective effects of CREB and raise the possibility that decreased mitochondrial CREB activity contributes to ... Disruption of CREB activity in the mitochondria decreases the expression of a subset of mitochondrial genes, including the ND5 ... These results demonstrate that regulation of mitochondrial gene expression by mitochondrial CREB, in part, ...
... the transcriptional coactivator p300 and the closely related CREB-binding protein (CBP), having histone acetyltransferase (HAT ... The HIV-1 Tat protein binds to a stem-loop structure at the 5 end of viral mRNA and relieves this inhibition by inducing a ... Tat associates with HAT activity in human nuclear extracts and binds to p300 and CBP both in vitro and in vivo. Integrity of ... the integrated viral promoter is present in a chromatin-bound conformation and is transcriptionally silent in the absence of ...
CREB-Binding Protein; Fungal Proteins/genetics/metabolism; Nuclear Proteins/*chemistry/genetics/*metabolism; Trans-Activators/* ... Recombinant Fusion Proteins/metabolism; Proto-Oncogene Proteins/*metabolism; DNA/metabolism; DNA-Binding Proteins/metabolism; * ... Induction-independent recruitment of CREB-binding protein to the c-fos serum response element through interactions between the ... Induction-independent recruitment of CREB-binding protein to the c-fos serum response element through interactions between the ...
Creb-binding protein (CBP). Using GST pull-down assays, we demonstrate that MIER1 interacts with CBP and that this interaction ... Two major protein isoforms have been identified, MIER1α and β, which differ in their C-terminal sequence. Previously, we ... Histone 4 peptide binding assays demonstrate that this inhibition of HAT activity is not the result of interference with ... Functional analysis, using HEK293 cells, shows that the CBP bound to MIER1 in vivo has no detectable HAT activity. ...
CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including ... Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins ... CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including ... Multiple Functions of CREB-Binding Protein During Postembryonic Development: Identification of Target Genes ...
... cAMP response element-binding protein - CREB Polyclonal \ 18-662-20054 for more molecular products just contact us ... cAMP response element-binding protein - CREB Polyclonal. Related products : cAMP response element-binding protein - CREB ... cAMP response element-binding protein - CREB Polyclonal / Product Detail : 18-662-20054 cAMP response element-binding protein ... 12956721] Cyclic AMP response element-binding (CREB)-like proteins in a molluscan brain: cellular localization and learning- ...
Prothymosin α interacts with the CREBbinding protein and potentiates transcription. Zoe Karetsou, Adroniki Kretsovali, Carol ... ProTα binds to CBP in vivo and in vitro. (A) CBP colocalizes with ProTα. HeLa cells were double‐labeled with the polyclonal ... D) Identification of the CBP‐binding site within the ProTα molecule. ProTα (2 μg) was incubated with GST (lane 1), or the CBP ... PTα corresponds to 0.1 μg of purified protein. Detection was performed by ECL. The upper panel shows Coomassie Blue staining of ...
MOUSE CREB-binding protein gi,481698,pir,,S39161 CREB-binding protein - mouse gi,435855,gb,AAB28651.1, CREB-binding protein; ... CREB binding protein [Homo sapiens] gi,3023440,sp,Q92793,CBP_HUMAN CREB-binding protein gi,2443859,gb,AAC51770.1, CREB-binding ... gi,19547885,gb,AAL87531.1, CREB-binding protein [Mus musculus] gi,19547887,gb,AAL87532.1, CREB-binding protein [Mus musculus] ... CREB-binding protein like fami... 114 1e-24 gi,17554044,ref,NP_499201.1, CREB-binding protein like fami... 79 5e-14 gi,482238, ...
  • The family members share a 300-aa Rel homology region that is responsible for protein dimerization, nuclear localization, and DNA binding to κB elements in the enhancer regions of target genes. (pnas.org)
  • Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. (rcsb.org)
  • Members of the CREB binding protein (CBP)/p300 family have been shown to influence development by (1) acting as bridging molecules between the basal transcriptional machinery and specific DNA-binding transcription factors, (2) physically interacting with terminal members of signaling cascades, (3) acting as transcriptional coactivators of downstream target genes, and (4) playing a key role in chromatin remodeling. (genetics.org)
  • Like other nuclear receptors, the peroxisome proliferator-activated receptors (PPARs) use a wide variety of protein-protein interactions to properly regulate transcription of target genes. (nih.gov)
  • One of the genes identified as a PPARalpha-associated protein by interaction cloning was the CREB-binding protein/p300-interacting transactivator with ED-rich tail 2 (CITED2, also called p35srj/mrg1/msg1). (nih.gov)
  • Genes that were affected by PPARalpha ligands in a CITED2-modulatory manner include angiopoietin-like protein 4, forkhead C2, hypoxia-inducible factor-1alpha, and MAPK phosphatase 1. (nih.gov)
  • Functionally important binding sites for GATA-1 are found in the regulatory regions of essentially all erythroid-cell-specific genes ( 30 ). (asm.org)
  • CREB is a bZIP transcription factor that activates target genes through cAMP response elements. (biomol.com)
  • CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. (biomol.com)
  • Multiple functions of CREB-binding protein during postembryonic development: identification of target genes. (biomedcentral.com)
  • Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins associated with growth and development. (uky.edu)
  • Sterol regulatory element-binding proteins (SREBPs) activate promoters for key genes of metabolism to keep pace with the cellular demand for lipids. (elsevier.com)
  • cAMP can stimulate the transcription of many activity-dependent genes via activation of the transcription factor, cAMP response element-binding protein (CREB). (jneurosci.org)
  • The CREBBP gene provides instructions for making CREB binding protein, which regulates the activity of many genes in tissues throughout the body. (medlineplus.gov)
  • HNF-1α regulates the expression of many liver-specific genes by directly binding to the promoter and enhancer regions, including albumin, fibrinogen, and α 1 -anti-trypsin genes (consensus site: GTTAATNATTAAC, where N = A, C, G, T, or no nucleotide) ( 8 , 9 ). (diabetesjournals.org)
  • The transcription factor BTB and CNC homology 1 (Bach1) is widely expressed in most mammalian tissues and functions primarily as a transcriptional suppressor by heterodimerizing with small Maf proteins and binding to Maf recognition elements in the promoters of targeted genes. (hindawi.com)
  • Once formed, the Bach1-Maf heterodimers inhibit the transcription of many oxidative stress-response genes, including heme oxygenase-1 (HO-1) [ 2 ] and NADPH quinone oxidoreductase 1(NQO1) [ 3 ], by binding to Maf recognition elements (MAREs) in the gene promoters. (hindawi.com)
  • Bach1 competes with nuclear factor (erythroid-derived 2)-like-2 (Nrf2) for binding to the MAREs in oxidative stress-response genes. (hindawi.com)
  • C/EPBβ binding to these promoters is associated with recruitment of the coactivator CBP/p300, histone H4 acetylation, and maximal activation of E2F target genes. (elsevier.com)
  • Moreover, binding of CBP/p300 to E2F targets is markedly reduced in C/EBPβ null mice, resulting in reduced expression of E2F regulated genes. (elsevier.com)
  • Ionov, Y, Matsui, SI & Cowell, JK 2004, ' A role for p300/CREB binding protein genes in promoting cancer progression in colon cancer cell lines with microsatellite instability ', Proceedings of the National Academy of Sciences of the United States of America , vol. 101, no. 5, pp. 1273-1278. (elsevier.com)
  • The serum response factor is a member of the MADS-box ( M CM1, A gamous, D eficiens, and S RF) box superfamily of transcription factors, [5] binding to the serum response element (SRE) in the promoter region of target genes. (wikiversity.org)
  • The murine double minute ( mdm2 ) oncogene , which codes for the Mdm2 protein, was originally cloned, along with two other genes (mdm1 and mdm3) from the transformed mouse cell line 3T3-DM. (wikipedia.org)
  • Simultaneously, a number of other DNA binding transcription factors are expressed and/or activated under redox control, such as Nuclear Respiratory Factor-1 (NRF-1), and lead to the induction of genes involved in both intracellular and mitochondria-specific repair mechanisms. (frontiersin.org)
  • RNA pol II transcribes all protein coding, snoRNA and snRNA genes d. (brainscape.com)
  • This causes a β-catenin cytoplasmic accumulation allowing entry into nucleus, where it exerts its action by binding to a clump of molecules and transcription factors, allowing to express the target genes, including the incretin hormones. (isciii.es)
  • Esto produce una acumulación de esta proteína en el citoplasma celular y permite su entrada al núcleo, donde ejerce su acción al unirse a una serie de moléculas y factores de transcripción, permitiendo de este modo que se expresen los genes diana, entre los que se encuentran los de las hormonas incretinas. (isciii.es)
  • Here we demonstrate that transcriptional activation by HIV-1 Tat involves p300 or the related cellular transcriptional coactivator CREB binding protein (CBP). (asm.org)
  • Transactivation by both components of this complex, serum response factor (SRF) and the ternary complex factor Elk-1, can be potentiated by the coactivator CREB-binding protein (CBP). (cnrs.fr)
  • 2011) A small molecule binding to the coactivator CREB-binding protein blocks apoptosis in cardiomyocytes. (guidetopharmacology.org)
  • The CREB protein domains, KIX, TAZ1 and TAZ2, each bind tightly to a sequence spanning both transactivation domains 9aaTADs of transcription factor p53. (wikipedia.org)
  • First isolated as a nuclear protein [Ref] that binds to cAMP-response element-binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. (wikipedia.org)
  • Cyclic AMP response element-binding protein (CREB) is a widely expressed transcription factor whose role in neuronal protection is now well established. (semanticscholar.org)
  • A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. (childrensmercy.org)
  • We have reported that the nuclear localizing protein p30 II of HTLV-1 functions as a transcription factor, differentially modulates CREB-responsive promoters, and is critical for maintenance of proviral loads in rabbits. (elsevier.com)
  • Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and transcription factor binding . (genecards.org)
  • Some of these putative co-regulatory proteins are members of transcription factor families that all bind to the same DNA sequence elements in vitro and are often expressed in the same cells. (elsevier.com)
  • CREB is a transcription factor implicated in the pathogenesis of multiple cancers. (elsevier.com)
  • Phosphorylation of ETS transcription factor ER81 in a complex with its coactivators CREB-binding protein and p300. (edu.sa)
  • The transcriptional co-activators and histone acetyltransferases p300/CREB-binding protein (CBP) interact with CITED2, a transcription factor AP-2 (TFAP2) co-activator. (ox.ac.uk)
  • Human CREB-binding protein/p300-interacting transactivator with ED-rich tail (CITED) 4, a new member of the CITED family, functions as a co-activator for transcription factor AP-2. (ox.ac.uk)
  • This effect may be attributed to the suppression of GnRH receptor expression in the pituitary and downstream transcription factor c-Jun, Egr-1, Elk-1, and CREB expression in PKC-MAPK and cAMP-PKA signaling transduction pathways by mifepristone. (thefreedictionary.com)
  • The serum response factor (SRF) is a transcription factor protein that binds to the c-fos serum response element (SRE). (wikiversity.org)
  • Transcription factor p65 also known as nuclear factor NF-kappa-B p65 subunit is a protein that in humans is encoded by the RELA gene. (wikipedia.org)
  • CREB-binding protein (CBP) is a coactivator that interacts with the cAMP-response element binding protein (CREB), a process dependent on the cAMP-dependent protein kinase A and its phosphorylation of CREB ( 21 , 22 ). (pnas.org)
  • Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. This modification stimulates interaction with one or more of the general transcription factors or, alternatively, allows recruitment of a co-activator. (nih.gov)
  • CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. (biomol.com)
  • These results are consistent with a model in which CBP is constitutively bound to the SRE in a higher order complex that would facilitate the rapid transcriptional activation of c-fos by signaling-driven phosphorylation. (cnrs.fr)
  • Regulation of genotoxic stress response by homeodomain-interacting protein kinase 2 through phosphorylation of cyclic AMP response element-binding protein at serine 271. (edu.sa)
  • cAMP activates cAMP-dependent protein kinase (PKA), which can directly modulate effector proteins by phosphorylation at PKA-specific serine or threonine residues or can alter the levels of the effector proteins themselves by modulating gene expression. (jneurosci.org)
  • Although CREB phosphorylation is necessary, it may not be sufficient to initiate gene transcription. (jneurosci.org)
  • Another signaling pathway activated by growth factor receptors involves the phosphorylation of signal transducers and activators of transcription (STAT). The duration and intensity of these signals are regulated by phosphatases and proteins that induce the activation of the JAK/STAT pathway, named suppressors of cytokine signaling (SOCS), and particularly SOCS1, a negative regulator of interleukin-6 (IL-6) and interferon (IFN-γ) signaling 8 . (nature.com)
  • Mapping the interaction of corepressor proteins (HDAC1, silencing mediator of retinoid and thyroid receptor/nuclear corepressor of retinoid receptor, and Sin3a) and HATs to IRF5 revealed distinct differences, including the dependence of IRF5 phosphorylation on HAT association resulting in IRF5 acetylation. (jimmunol.org)
  • Phosphorylation of at least two serine residues (Ser 276 and Ser 311 ) has also been shown to be important for interaction of NF-κB with the histone acetyltransferase (HAT) CREB-binding protein (CBP)/p300 ( 20 , 21 ). (jimmunol.org)
  • The paricalcitol-induced EP4 activation mediated phosphorylation of Akt and CREB and inhibited phosphorylation and nuclear translocation of p65 NF-[kappa]B in in vitro and in vivo models. (thefreedictionary.com)
  • Phosphorylation of CREB at the Ser133 site is known to play an important role in regulating the memory process. (thefreedictionary.com)
  • Both classes show an increase in binding after phosphorylation of CREB by protein kinase A (PKA). (uni-muenchen.de)
  • An in vivo phosphorylation-dependent change in binding of CREB increases the occupancy of weak binding sites used for transactivation, such as the TATCRE, while high affinity sites may have constitutive binding of transcriptionally active and inactive CREB dimers, as demonstrated by in vivo footprinting at the PEPCK CRE. (uni-muenchen.de)
  • Phosphorylation of serine 311 by protein kinase C zeta type (PKCζ) serves the same purpose. (wikipedia.org)
  • The fact that RELA can be modified by a collection of kinases via phosphorylation at different sites/regions within the protein under different stimulations might suggest a synergistic effect of these modifications. (wikipedia.org)
  • Phosphorylation at these sites enhances NF-κB transcriptional response via tightened binding to transcription coactivators. (wikipedia.org)
  • In vivo studies revealed that RELA is also under acetylation modification in the nucleus, which is just as important as phosphorylation as a post-translational modification of proteins. (wikipedia.org)
  • Recent results suggest that novel CBP-mediated post-translational N-glycosylation activity alters the conformation of CBP-interacting proteins, leading to regulation of gene expression, cell growth and differentiation, Homeodomain interacting protein kinase 2 (HIPK2) phosphorylates several regions of CBP close to the N-terminal and close to the C-terminal region as well. (wikipedia.org)
  • Here we report that CREB phosphorylated by protein kinase A binds specifically to a nuclear protein of M(r) 265K which we term CBP (for CREB-binding protein). (nih.gov)
  • Fusion of a heterologous DNA-binding domain to the amino terminus of CBP enables the chimaeric protein to function as a protein kinase A-regulated transcriptional activator. (nih.gov)
  • Exposure of monocytes and macrophages to LPS results in activation of the mitogen-activated protein kinase (MAPK) pathway, including the extracellular signal-related kinase (ERK), c-jun NH 2 -terminal kinase (JNK), and p38 cascades (reviewed in reference 12 ). (asm.org)
  • Kovács KA, Steinmann M, Halfon O, Magistretti PJ, Cardinaux J-R (2015) Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2. (edu.sa)
  • Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2. (edu.sa)
  • We found that the p90 ribosomal S6 kinase 2 (RSK2)-cAMP response element-binding protein (CREB) pathway is commonly activated in diverse metastatic human cancer cells, leading to up-regulation of a CREB transcription target Fascin-1. (novartis.com)
  • Greenberg, "Coupling of the RAS-MAPK pathway to gene activation by RSK2, a growth factor-regulated CREB kinase," Science, vol. (thefreedictionary.com)
  • Brain-derived neurotrophic factor (BDNF) and its receptor tropomyosin receptor kinases (TrkB), as well as extracellular signal-regulated kinase (ERK) and its downstream target c-AMP-responsive element binding protein (CREB) are strongly associated with the transmission of nociceptive information. (biologists.org)
  • for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). (wikiversity.org)
  • Widely used inhibitors of cAMP-dependent protein kinase (PKA) have nonspecific side effects. (sciencemag.org)
  • Triggered by lipopolysaccharide (LPS), protein kinase A (PKA) specifically phosphorylates serine 276 in the RHD domain in the cytoplasm, controlling NF-κB DNA-binding and oligomerization. (wikipedia.org)
  • CREBBP (CREB Binding Protein) is a Protein Coding gene. (genecards.org)
  • Mutations in the CREBBP (CREB-binding protein gene) cause Rubinstein-Taybi syndrome (RSTS). (cdc.gov)
  • Crebbp (CREB binding protein, also known as CBP) is a protein acetyltransferase that is required for normal development and acts as a tumor suppressor. (jax.org)
  • The KIX domain mediates binding to HIV-1 Tat. (rcsb.org)
  • The carboxy-terminal region of p300, which binds to E1A, was shown to bind specifically to the highly conserved basic domain of Tat, which also mediates binding to the Tat-responsive region RNA stem-loop structure. (asm.org)
  • The family of Smad proteins mediates transforming growth factor-beta (TGF-beta) signaling in cell growth and differentiation. (thebiogrid.org)
  • Mitochondrial cyclic AMP response element-binding protein (CREB) mediates mitochondrial gene expression and neuronal survival. (semanticscholar.org)
  • Collectively, our results demonstrate that CBP/p300 is a cellular protein target for HTLV-1 p30 II and mediates its transcriptional effects in vivo. (elsevier.com)
  • The N-terminal region of Bach1 contains a BTB/POZ domain, which functions as a protein interaction motif, while the C-terminal bZip domain binds to DNA [ 1 ] and mediates the heterodimerization of Bach1 with small Maf proteins (e.g. (hindawi.com)
  • The p65 subunit, similar to two others in the κB family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein ( 8 ). (pnas.org)
  • In addition, PML and the transactivation cofactor, CREB binding protein (CBP), colocalize within the nucleus. (pnas.org)
  • Importantly, the CBP-binding activity of Nrf2 deletion mutants positively correlated with their transactivation activity. (nih.gov)
  • Our results indicate that Nrf2 exploits the cooperative binding of two independent transactivation domains to CBP in the acquisition of a potent transactivation activity. (nih.gov)
  • DNA binding and glutathione S-transferase pull-down assays demonstrate that binding requires Elk-1(1-212) but not the C-terminal transactivation domain. (cnrs.fr)
  • Gal4(BD)-p30 II -mediated transactivation was enhanced following exogenous expression of p300 and was competitively repressed by the p300 binding protein, adenovirus E1A, and E1ACR2 (mutated for retinoblastoma binding but retaining p300 binding). (elsevier.com)
  • In contrast, E1ACR1 (mutated for p300 binding) failed to alter Gal4(BD)-p30 II -mediated transactivation. (elsevier.com)
  • Notably, a series of posttranslational modifications of NF-κB proteins after IκB degradation appears to regulate nuclear localization, DNA binding, and transcriptional transactivation ( 18 , 19 ). (jimmunol.org)
  • Like other proteins in this complex, RELA contains a N-terminal REL-homology domain (RHD), and also a C-terminal transactivation domain (TAD). (wikipedia.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human CREB Binding Protein (CREBBP) in samples from tissue homogenates or other biological fluids. (ialltjournal.org)
  • We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding protein (CBP) and p300. (elsevier.com)
  • The nuclear localization signal of NF-κB is masked by the binding of an inhibitory protein, IκB, sequestering NF-κB in the cytoplasm in unstimulated cells. (pnas.org)
  • We have previously shown that CREB-binding protein (CBP/p300) act as an important SOX9 co-activator during chondrogenesis. (elsevier.com)
  • This protein shares regions of very high sequence similarity with protein EP300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. (wikipedia.org)
  • In diffuse large B-cell lymphoma (DLBCL), inactivating mutations of the histone acetyltransferases CREB-binding protein (CBP) and EP300 are common. (lu.se)
  • Our results suggest that depletion of CBP and EP300 levels leads to a strong reduction of CD20 expression, accompanied by reduced binding of PU.1 to the CD20 promoter. (lu.se)
  • In CBP-depleted, but not EP300-depleted cells, increased binding of FOXO1 to the CD20 promoter was observed. (lu.se)
  • The CREB protein carries out its function by activating transcription, where interaction with transcription factors is managed by one or more CREB domains: the nuclear receptor interaction domain (RID), the KIX domain (CREB and MYB interaction domain), the cysteine/histidine regions (TAZ1/CH1 and TAZ2/CH3) and the interferon response binding domain (IBiD). (wikipedia.org)
  • The small-molecule ICG-001 binds cAMP-responsive element binding (CREB)-binding protein (CBP) to disrupt its interaction with β-catenin and inhibit CBP function as a coactivator of Wnt/β-catenin-mediated transcription. (aacrjournals.org)
  • The ability of Tat to interact physically and functionally with this coactivator provides a mechanism to assemble a basal transcription complex which may subsequently respond to the effect of Tat on transcriptional elongation and represents a novel interaction between an RNA binding protein and a transcriptional coactivator. (asm.org)
  • Recruitment of human cyclin T1 to nuclear bodies through direct interaction with the PML protein. (semanticscholar.org)
  • To further explore the role of MIER1 in chromatin remodeling, we investigated the functional interaction of MIER1 with the histone acetyltransferase (HAT), Creb-binding protein (CBP). (biomedcentral.com)
  • [10] Interaction of SRF with other proteins, such as steroid hormone receptors, may contribute to regulation of muscle growth by steroids. (wikiversity.org)
  • Using either endogenous p53 or a second fluorescently tagged fusion of p53 with the rotavirus NSP5 protein, we demonstrated p53 oligomerization as well as p53 association with another of its cellular interaction partners, the CREB-binding proteins, within the inclusions. (mcponline.org)
  • Numerous methods are available for addressing whether proteins associate either directly ("interaction") or indirectly (through bridging molecules), but many of these methods can be technically demanding, can be limited by the strength of the association, or cannot resolve whether the association indeed occurs inside cells. (mcponline.org)
  • Nuclear factor κB binding sites were shown to be primarily responsible for the positive activity contributed by the HS1,2 and HS4 regions, and we observed the in vivo interaction of these factors with the human immunoglobulin heavy chain gene enhancer regions in t(14;18) cells. (aacrjournals.org)
  • RHD is involved in DNA binding, dimerization and NF-κB/REL inhibitor interaction. (wikipedia.org)
  • This protein has also been shown to possess acetyltransferase activity, further implicating it in the accessibility of chromatin/histones for active transcription ( 32 , 33 ). (pnas.org)
  • The CREB binding protein ensures the DNA is ready for transcription by attaching a small molecule called an acetyl group to proteins called histones (a process called acetylation). (medlineplus.gov)
  • Histones are structural proteins that bind DNA and give chromosomes their shape. (medlineplus.gov)
  • The CREB-binding protein (CBP) pathway plays an important role in transcription and activity of acetyltransferase that acetylates lysine residues of histones and nonhistone proteins. (cdc.gov)
  • Besides histones, HATs and HDACs may also act on nonhistone proteins such as transcription factors. (jimmunol.org)
  • Lysine propionylation and butyrylation are protein modifications that were recently identified in histones. (mcponline.org)
  • BACKGROUND: Post-translational modifications (PTMs) of histones and other proteins are perturbed in tumours. (biomedsearch.com)
  • Recognition of acetyl-lysine by bromodomain proteins is not limited to histones. (cellsignal.com)
  • In LPS-stimulated macrophages, the ERK substrates Ets and Elk-1 bind to the TNF-α promoter in vivo. (asm.org)
  • The transcription factors ATF-2, c-jun, Egr-1, and Sp1 are also inducibly recruited to the TNF-α promoter in vivo, and the binding sites for each of these activators are required for LPS-stimulated TNF-α gene expression. (asm.org)
  • The finding that a distinct set of transcription factors associates with a fixed set of binding sites on the TNF-α promoter in response to LPS stimulation lends new insights into the mechanisms by which complex patterns of gene regulation are achieved. (asm.org)
  • We identify TNF-α promoter elements critical for LPS induction of the gene and demonstrate that two Sp1 binding sites and three Ets binding sites, in addition to a cyclic AMP response element (CRE)-like site and an Egr site, are critical for LPS induction of the TNF-α gene. (asm.org)
  • Consistent with this functional analysis of the TNF-α promoter, using chromatin immunoprecipitation and formaldehyde crosslinking (ChIP) assays, we directly detect LPS-inducible binding of the transcription factors ATF-2, c-jun, Ets-1 and -2, Elk-1, Egr-1, and Sp1 to the endogenous TNF-α promoter. (asm.org)
  • Thus, a unique TNF-α enhancer complex, including Ets, Elk-1, Sp1, ATF-2-Jun, and the coactivator proteins CBP and p300, is assembled on the TNF-α promoter in LPS-stimulated monocytes. (asm.org)
  • Remarkably, a set of TNF-α promoter elements, which bind NFAT upon induction of the gene by calcineurin-dependent stimuli, also bind the ERK-targeted Ets and Elk proteins and are required in LPS-stimulated TNF-α gene expression. (asm.org)
  • HIV-1 tat transactivator recruits p300 and CREB-binding protein histone acetyltransferases to the viral promoter. (semanticscholar.org)
  • In cells infected with HIV type 1 (HIV-1), the integrated viral promoter is present in a chromatin-bound conformation and is transcriptionally silent in the absence of stimulation. (semanticscholar.org)
  • However, inhibition is not dependent upon the presence of a CRE in the promoter, and does not involve heterodimer formation between CREB and the activator. (umassmed.edu)
  • Human T-lymphotropic virus type 1 (HTLV-1) transcription is controlled, in part, by the CREB/ATF family of transcription factors which bind promoter sequences and function as complexes with the viral oncogenic protein Tax. (elsevier.com)
  • In each SREBP-regulated promoter, at least one ubiquitous co-regulatory factor that binds to a neighboring recognition site is also required for efficient gene induction. (elsevier.com)
  • We have used the chromatin immunoprecipitation (ChIP) technique coupled with a transient complementation assay in Drosophila SL2 cells to directly compare the ability of two members of the CREB/ATF family to function as co-regulatory proteins for SREBP-dependent activation of the HMG-CoA reductase promoter. (elsevier.com)
  • The mouse CREB gene is expressed from a promoter that is situated in a CpG island. (houstonmethodist.org)
  • The promoter contains no TATA or CCAAT box homologies but has a number of putative binding sites for the acidic transcriptional activator Sp1 and a 9 11 match with the initiator region. (houstonmethodist.org)
  • Binding of the transcription factors PU.1 and FOXO1 to the CD20 promoter was determined by chromatin immunoprecipitation coupled with quantitative polymerase chain reaction. (lu.se)
  • We report that CCAAT/enhancer-binding protein (C/EBP)-β interacts with ARE/EpRE in the rGST-Ya promoter and that aryl hydrocarbon receptor (AhR) is present within the protein complex binding to the C/EBP site. (elsevier.com)
  • Inhibition requires that the CREB dimerization and DNA-binding domains are intact. (umassmed.edu)
  • A small molecule inhibitor (I-CBP112) binding to the bromodomain domain of CBP/p300 has been developed for leukemia therapy. (wikipedia.org)
  • Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB. (elsevier.com)
  • Our working model suggests that Smad protein activity is delicately balanced by Ski and CBP in the TGF-beta pathway. (thebiogrid.org)
  • Novel genetic variants in HDAC2 and PPARGC1A of the CREB-binding protein pathway predict survival of non-small-cell lung cancer. (cdc.gov)
  • Accumulating evidence suggests that O -GlcNAc transferase (OGT), an enzyme responsible for modification of proteins with N -acetylglucosamine, may act as a nutrient sensor that links hexosamine biosynthesis pathway to oncogenic signaling and regulation of factors involved in glucose and lipid metabolism. (frontiersin.org)
  • There is also evidence that glucose, through the hexosamine pathway, can induces autocrine activation of Wnt signalling pathway by stimulating secretion of Wnt proteins. (isciii.es)
  • Finally, CBP and p300 were recently shown to acetylate nonhistone proteins such as the tumor suppressor protein p53 ( 16 ), the erythroid Kruppel-like factor (EKLF) ( 48 ), and the basal transcription factors, TFIIE and TFIIF ( 19 ). (asm.org)
  • A stimulus-specific role for CREB-binding protein (CBP) in T cell receptor-activated tumor necrosis factor alpha gene expression. (duke.edu)
  • The present work aimed at analyzing the SOCS1 cell signaling and expression of proteins relevant to tumor development. (nature.com)
  • We also observed that the protein expression patterns of RSK2 and Fascin-1 correlate in primary human tumor tissue samples from head and neck squamous cell carcinoma patients. (novartis.com)
  • The transport via exosomes of EBV-regulated miRNAs and viral proteins contributes to the construction and modification of the inflammatory tumor microenvironment. (springer.com)
  • Mdm2 protein functions both as an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and as an inhibitor of p53 transcriptional activation. (wikipedia.org)
  • The E3 ubiquitin ligase MDM2 is a negative regulator of the p53 tumor suppressor protein. (wikipedia.org)
  • acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). (rcsb.org)
  • Acetylation in vitro did not alter the ability of GATA-1 to bind DNA, and mutations in either motif did not affect DNA binding of GATA-1 expressed in mammalian cells. (asm.org)
  • Therefore, the binding of p53 serves to modify SV40 LT by targeting CBP and p300 binding to direct the acetylation of SV40 LT. (thebiogrid.org)
  • We found that acetylation of E2F1 is, instead, required to stabilize the protein in response to doxorubicin. (elsevier.com)
  • We suggest that MOZ may represent a chromatin-associated acetyltransferase, and raise the possibility that a dominant MOZ-CBP fusion protein could mediate leukaemogenesis via aberrant chromatin acetylation. (elsevier.com)
  • For instance, lysine 221 acetylation facilitates RELA dissociation from IκBα and enhances its DNA-binding affinity. (wikipedia.org)
  • Collectively, these proteins interact with components of the basal transcriptional apparatus to effect dramatic changes in gene expression (for review, see ref. 1 ). (pnas.org)
  • A second class of proteins important in the initiation of transcription by RNA polymerase II are the coactivators (i.e., proteins that bridge the transcriptional activators and the components of the basal transcriptional apparatus). (pnas.org)
  • p300/CBP binds not only to the activation domain of some transcription factors, such as CREB and the glucocorticoid receptor, but also to multiple components of the basal transcriptional machinery, including the TFIIB ( 15 ) and RNA polymerase II holoenzyme complex ( 17 ), suggesting that p300/CBP serves as a molecular bridge between the transcription factors and the basal transcriptional machinery. (diabetesjournals.org)
  • Solution NMR structure of ischemin bound to the bromodomain of CBP. (guidetopharmacology.org)
  • Identified in over one hundred proteins from yeast to man, the approximately 110 amino acid bromodomain can bind to acetylated lysine residues. (cellsignal.com)
  • For example, the bromodomain of CREB binding protein transcriptional coactivator (CBP) allows for recognition of p53 with acetylated Lys382. (cellsignal.com)
  • Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME. (childrensmercy.org)
  • Comprehensive screening of CREB-binding protein gene mutations among patients with Rubinstein-Taybi syndrome using denaturing high-performance liqu. (cdc.gov)
  • It interacts with a significant percentage of mammalian transcriptional regulatory proteins and mutations in the gene have been found in hematopoietic and epithelial tumors. (jax.org)
  • Similar polyacidic tracks are found in several nuclear proteins whose functions are linked to chromatin decondensation, nucleosome assembly/disassembly and establishment of chromatin structures competent for transcription ( Earnshaw, 1987 ). (embopress.org)
  • To date, the cellular cofactors that bind to Tat have not been implicated in chromatin remodeling. (asm.org)
  • The highly conserved coadapters CREB binding protein (CBP) and p300 form complexes with CREB as well as other DNA binding transcription factors to modulate chromatin remodeling and thus transcription. (elsevier.com)
  • The lysine acetyltransferase CREB binding protein (CBP) is required for chromatin changes and transcription at many gene promoters. (movd2016.org)
  • Bromodomains are generally found in proteins that regulate chromatin structure and gene expression, such as histone acetyltransferases and the ATPase component of certain nucleosomes-remodeling complexes. (cellsignal.com)
  • The adenovirus E1A 12S protein, which complexes with CBP and p300, inhibited p65-dependent gene expression. (pnas.org)
  • We propose that CBP may participate in cAMP-regulated gene expression by interacting with the activated phosphorylated form of CREB. (nih.gov)
  • 2001). RNA-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. (rutgers.edu)
  • For PML-green fluorescent protein (GFP) expression, cells were microinjected with a CMX PML-GFP DNA expression construct at 50-200 ng/μl and incubated overnight to allow for expression. (pnas.org)
  • The expression patterns of specific DNA-binding factors that control eye development add an additional layer of complexity ( K umar and M oses 1997 ). (genetics.org)
  • In an attempt to identify novel PPAR-interacting proteins, a cDNA expression library was screened with bacterially expressed PPARalpha. (nih.gov)
  • To determine whether CITED2 affects endogenous gene expression, this protein was stably overexpressed (CITED2+) or repressed by small inhibitor RNA (CITED2-) in immortalized mouse hepatocytes. (nih.gov)
  • This article examined the expression levels of the p53 protein and CBP as well as their diagnostic value in a biopsy sample. (koreamed.org)
  • Strikingly, Ets and Elk-1 bind to two TNF-α nuclear factor of activated T cells (NFAT)-binding sites, which are required for calcineurin and NFAT-dependent TNF-α gene expression in lymphocytes. (asm.org)
  • Calcineurin targets the nuclear factor of activated T cells (NFAT) family of proteins (reviewed in references 11 and 38 ), which are critical for TNF-α gene expression by calcineurin-dependent signal transduction pathways ( 15 , 48 , 49 ). (asm.org)
  • Transient expression of the constructs in COS-1 (Fig. 3C) or HEK293 cells (not really proven) revealed that while every one of the truncated CBP protein had been nuclear just full-length CBP demonstrated a solid association with nuclear physiques. (movd2016.org)
  • Northern blot analysis indicated that expression of the CREB gene is almost ubiquitous with expression at differing levels of multiple transcripts. (houstonmethodist.org)
  • Site-direct mutagenesis and footprint analyses showed that the HNF-1α binding site (+200 to +218) is critical in human GLUT2 gene expression. (diabetesjournals.org)
  • Under normal physiological conditions, nuclear Nrf2 contributes to vascular protection by inducing expression of the glutamate cysteine ligase modulatory subunit (GCLM) and the light chain component of system x c − (xCT) in human endothelial cells, while cytoplasmic Nrf2 is bound and inhibited by Kelch-like ECH-associated protein 1 (Keap1) [ 15 ]. (hindawi.com)
  • Expression of mutant CREB shifted the dose-learning curve for isoproterenol to the right such that a higher dose was required to induce learning. (mun.ca)
  • Expression of CREB shifted the dose-learning curve for isoproterenol to the left, with a lower dose now producing learning. (mun.ca)
  • Phosphorylated ERK and CREB Participate in LPA-Stimulated DR6 Expression. (thefreedictionary.com)
  • subpart (b) is the expression levels of pERK, ERK, pCREB, and CREB proteins. (thefreedictionary.com)
  • Results showed that BDNF, TrkB, phosphor(p)-ERK and p-CREB were up-regulated in the brain neurons of both male and female rats with NTG-induced migraine compared to non-migraine control, whereas their expression levels were decreased in headache-free intervals of the migraine compared to migraine attacks. (biologists.org)
  • Female ovariectomized rats showed significant reduction in the expression of BDNF, TrkB, p-CREB and p-ERK in both attacks and intervals of NTG-induced migraine, relative to rats that have their ovaries. (biologists.org)
  • Myocyte enhancer factor-2 (MEF2) proteins are a family of transcription factors which through control of gene expression are important regulators of cellular differentiation and consequently play a critical role in embryonic development. (wikiversity.org)
  • This research investigated the effects of administration of high doses of DS seeds on the expression of CREB protein in both male and female rats' frontal cortices and its implication in addiction and neurodegeneration. (scirp.org)
  • The rats were euthanized and an analysis of variance (ANOVA) was computed to detect a significant main difference of DS effect on CREB expression for each group, while post hoc Bonferroni Test compared CREB protein expression between male and female groups. (scirp.org)
  • Result: There were significant differences in the expression of CREB protein between the sub-groups and between the male and female rats of treated sub-group (p (scirp.org)
  • We have previously shown that the transcriptional cofactor CREB-binding protein (CBP) binds to the zinc finger domain of GATA-1, markedly stimulates the transcriptional activity of GATA-1, and is required for erythroid differentiation. (asm.org)
  • Our previous studies indicated that a widely expressed transcriptional cofactor, CREB-binding protein (CBP), is a potent coactivator of GATA-1 ( 4 ). (asm.org)
  • Neh4 and Neh5 both individually and cooperatively bind to CBP (CREB (cAMP Responsive Element Binding protein) Binding Protein). (nih.gov)
  • CREB-binding protein, also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene. (wikipedia.org)
  • Mouse double minute 2 homolog ( MDM2 ) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene . (wikipedia.org)
  • The CREB-binding protein(CBP) is a transcriptional co-activators of various sequence-specific DNA-binding transcription factors and is involved in a wide variety of cellular activities, such as DNA repair, cell growth, differentiation, and apoptosis. (koreamed.org)
  • CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. (biomol.com)
  • Among the factors associated with the basal transcription complex, p300 and the related CREB binding protein (CBP) ( 2 ) have emerged as coactivators for a broad group of cellular transcription factors (for reviews, see references 5 and 34 ). (asm.org)
  • Protein-protein associations are vital to cellular functions. (mcponline.org)
  • Background: cAMP response element-binding protein (CREB) is one of the cellular transcription factors found in neurons. (scirp.org)
  • Positional cloning on chromosome 16 implicates the CREB-binding protein (CBP), a transcriptional adaptor/coactivator protein. (elsevier.com)
  • At the chromosome 8 breakpoint we identify a novel gene, MOZ, which encodes a 2,004-amino-acid protein characterized by two C4HC3 zinc fingers and a single C2HC zinc finger in conjunction with a putative acetyltransferase signature. (elsevier.com)
  • Finally Creb-1, the CREB locus, was mapped to the proximal region of mouse chromosome 1. (houstonmethodist.org)
  • Here we report that CREB is present in the mitochondrial matrix of neurons and that it binds directly to cyclic AMP response elements (CREs) found within the mitochondrial genome. (semanticscholar.org)
  • Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. (umassmed.edu)
  • Here, we have examined the effects of CsA on the DNA-binding activity of the cyclic AMP response element-binding protein (CREB) and cell viability, and the effects of CREB on cathepsin B and L synthesis and activity in HGFs. (elsevier.com)
  • Cyclic AMP treatment of hepatoma cells leads to increased protein binding at the cyclic AMP response element (CRE) of the tyrosine aminotransferase (TAT) gene in vivo, as revealed by genomic footprinting, whereas no increase is observed at the CRE of the phosphoenolpyruvate carboxykinase (PEPCK) gene. (uni-muenchen.de)
  • The NICD translocates to the nucleus, where it forms a complex with the DNA binding protein CSL, displacing a histone deacetylase (HDAc)-co-repressor (CoR) complex from CSL. (genome.jp)
  • Human immunodeficiency virus type 1 (HIV-1) encodes the transactivator protein Tat, which is essential for viral replication and progression to disease. (asm.org)
  • The HIV-1 Tat protein binds to a stem-loop structure at the 5' end of viral mRNA and relieves this inhibition by inducing a remodeling of the nucleosome arrangement downstream of the transcription-initiation site. (semanticscholar.org)
  • Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. (elsevier.com)
  • Zta-mediated viral reactivation and transcriptional activation were both significantly inhibited by coexpression of the E1A 12S protein but not by an N-terminal deletion mutation of E1A (E1AΔ2-36), which fails to bind CBP. (elsevier.com)
  • Orthoreovirus protein μNS recruits the orthoreovirus RdRp λ3 to cytoplasmic viral factories or FLS. (mcponline.org)
  • This cell damage caused by oxidative and nitrosative stress leads to mitochondrial protein, DNA, and lipid modifications, which inhibits energy production and contractile function, potentially leading to cell necrosis and/or apoptosis. (frontiersin.org)
  • Importantly, we demonstrate that p30 II colocalizes with p300 in cell nuclei and directly binds to CBP/p300 in cells. (elsevier.com)
  • The cAMP response element binding protein (CREB)-binding protein (CBP)/p300 family of coactivator proteins regulates gene transcription through the integration of multiple signal transduction pathways. (duke.edu)
  • The literature contains reports of the promyelocytic leukemia gene product PML protein functioning as an enhancer of transcription ( 1 ) as well as a suppressor of transcription and growth ( 2 ). (pnas.org)
  • Furthermore, assembly of the LPS-stimulated TNF-α enhancer complex is dependent upon the coactivator proteins CREB binding protein and p300. (asm.org)
  • Chen, YH & Ramos, KS 2000, ' A CCAAT/enhancer-binding protein site within antioxidant/electrophile response element along with CREB-binding protein participate in the negative regulation of rat GST-Ya, gene in vascular smooth muscle cells ', Journal of Biological Chemistry , vol. 275, no. 35, pp. 27366-27376. (elsevier.com)
  • In addition, two Sp1 binding sites in HS4 were also found to positively influence bcl-2 activity, and Sp1 was observed to interact with the human HS4 enhancer in vivo . (aacrjournals.org)
  • abstract = "In this paper we report the isolation and characterization of the mouse CREB gene. (houstonmethodist.org)
  • This is followed by the rapid translocation of NF-κB to the nucleus where it binds to specific κB elements ( 13 , 14 ). (pnas.org)
  • Prothymosin α (ProTα) is a histone H1‐binding protein localized in sites of active transcription in the nucleus. (embopress.org)
  • The IVT proteins had been useful for SUMO assays using recombinant after that … However deletion from the SUMOylated area did not influence the power of CBP to localise towards Ganetespib the nucleus (Figs. 2C and 4A) or its work Ganetespib as a coactivator. (movd2016.org)
  • It can translocate to the cell nucleus and bind to NF-κB p65. (nature.com)
  • The Ca 2+ /cAMP response element-binding protein (CREB) was initially identified as the main interlocutor in the dialogue between the synapse and the nucleus [1]. (wikiversity.org)
  • Transcriptional regulation, a critical control mechanism in fundamental biologic processes, requires the participation of several classes of proteins: those that bind specific DNA sequences, those that associate with transcriptional regulators through protein-protein interactions, known as transcriptional coactivators or corepressors, and those that perform an architectural function. (pnas.org)
  • These results suggest that CREB is essential for the CsA-mediated down-regulation of cathepsin B and L synthesis in HGFs. (elsevier.com)
  • Taken together, our findings for the first time link RSK2-CREB signaling to filopodia formation and bundling through the up-regulation of Fascin-1, providing a proinvasive and prometastatic advantage to human cancers. (novartis.com)
  • We report here that ProTα physically interacts with the CREB‐binding protein (CBP), which is a versatile transcription co‐activator. (embopress.org)
  • First, CBP interacts with TFIIB ( 23 ), TATA-binding protein ( 1 , 12 , 37 , 40 ), and RNA polymerase II ( 10 , 21 , 27 ) and could thus serve as a bridging molecule between DNA-bound transcription factors and the basal transcription machinery. (asm.org)
  • It interacts with a significant percentage of mammalian transcriptional regulatory proteins and has a CH1 domain that binds the C-terminal activation domain (C-TAD) of the hypoxia-inducible transcription factors HIF-1α and HIF-2α. (jax.org)
  • The proteins immunoprecipitated by anti-CREB antibodies were analyzed by ESI-Q-TOF-MS in Bel-7402 cells for three independent times. (hindawi.com)
  • b) Endogenous CREB was immunoprecipitated by anti-CREB antibodies in Bel-7402 and SMMC-7721 cells, and the indicated proteins were measured by WB. (hindawi.com)
  • Cells were obtained for IF by anti-CREB and corresponding antibodies. (hindawi.com)
  • Histone 4 peptide binding assays demonstrate that this inhibition of HAT activity is not the result of interference with histone binding. (biomedcentral.com)
  • Inhibition of Wnt/beta-catenin/CREB binding protein (CBP) signaling reverses pulmonary fibrosis. (qxmd.com)
  • As expected from this shift, CREB overexpression interfered with learning induced by stroking. (mun.ca)
  • They support evidence that CREB overexpression can be deleterious and suggest the hypothesis of an optimal pCREB window for learning. (mun.ca)
  • Overexpression of CREB-binding protein (CBP) nullified repression of rGST-Ya transcription. (elsevier.com)
  • The Notch proteins (Notch1-Notch4 in vertebrates) are single-pass receptors that are activated by the Delta (or Delta-like) and Jagged/Serrate families of membrane-bound ligands. (genome.jp)
  • Xie, F, Fan, Q, Li, BX & Xiao, X 2019, ' Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15 ', Journal of Medicinal Chemistry . (elsevier.com)
  • However, in mouse cortical neuron cultures, prior to synaptogenesis, neither cAMP nor dopamine, which acts via cAMP, stimulated CREB-dependent gene transcription when NR2B-containing NMDA receptors (NMDARs) were blocked. (jneurosci.org)
  • Here, we report that, in immature neurons from embryonic mouse cortex, prior to synaptogenesis, DA and cAMP do not stimulate CREB-dependent gene transcription when NMDA receptors (NMDARs) are blocked. (jneurosci.org)
  • Our results suggest that cAMP stimulates CREB-dependent gene transcription via a two-step process by inducing release of an excitatory amino acid, identified here as l -aspartate, which then activates NMDARs, leading to Ca 2+ entry and gene transcription. (jneurosci.org)
  • Members of the CREB-binding protein/p300-interacting transactivator with ED-rich tail (CITED) family bind CREB-binding protein and p300 with high affinity and regulate gene transcription. (ox.ac.uk)
  • On the basis of this function, CREB binding protein is called a histone acetyltransferase. (medlineplus.gov)
  • Bromodomains (BRDs) are epigenetic reader domains that selectively recognize acetylated lysine residues on the tails of histone proteins, and are the only known protein modules that can target acetylated lysine residues. (genecards.org)
  • Here we report identification of the first three in vivo non-histone protein substrates of lysine propionylation in eukaryotic cells: p53, p300, and CREB-binding protein. (mcponline.org)
  • We used mass spectrometry to map lysine propionylation sites within these three proteins. (mcponline.org)
  • We also identified the first two in vivo eukaryotic lysine propionyltransferases, p300 and CREB-binding protein, and the first eukaryotic depropionylase, Sirt1. (mcponline.org)
  • Several lysine residues in p53 C-terminus have been identified as the sites of ubiquitination, and it has been shown that p53 protein levels are downregulated by Mdm2 in a proteasome-dependent manner. (wikipedia.org)
  • The activity of various DNA-binding transcription factors is regulated by global transcriptional co-activators such as p300 and CREB-binding protein (CBP). (diabetesjournals.org)
  • Functional analysis, using HEK293 cells, shows that the CBP bound to MIER1 in vivo has no detectable HAT activity. (biomedcentral.com)
  • Because one copy of the CREBBP gene is altered in people with Rubinstein-Taybi syndrome, their cells make only half of the normal amount of functional CREB binding protein. (medlineplus.gov)
  • Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2. (ox.ac.uk)
  • The molecular components involved in the two protein modification pathways are unknown, hindering further functional studies. (mcponline.org)
  • Strikingly, CREB inhibits transcription of multiple activators, whose DNA-binding domains and activation regions are unrelated to one another. (umassmed.edu)
  • Androgen deprivation strongly inhibits the ligand-dependent nuclear accumulation of pathogenic AR protein, resulting in a striking improvement in neurological and histopathological findings of male mice. (jneurosci.org)
  • In both examples, we further localized a region of the recruited protein that is key to its recruitment. (mcponline.org)
  • These mechanisms are often mediated by histone linkers or by proteins associated with the recruitment of DNA-binding proteins, HDACI and II interacting proteins and transcriptional activators, coactivators or corepressors. (omicsonline.org)
  • Early odor preference learning in rats is associated with increases of phosphorylated CREB (pCREB) in mitral cells of the olfactory bulb. (mun.ca)
  • In the present study, herpes simplex virus expressing CREB (HSV-CREB) and dominant-negative mutant CREB (HSV-mCREB) have been injected into the bulb to assess a causal role for CREB and pCREB in this model. (mun.ca)
  • When learning occurred, with either CREB or mutant CREB, pCREB was observed to be elevated relative to the nonlearning LacZ control groups. (mun.ca)
  • Unexpectedly, with odor plus stroking in the nonlearning CREB group, the level of pCREB was also higher than with odor plus stroking in LacZ controls that did learn. (mun.ca)
  • The data demonstrate a causal role for CREB and pCREB in early mammalian odor preference learning, reinforcing CREB as a "universal" memory molecule. (mun.ca)
  • Figure 1 shows representative fluorescent bands obtained after immunoblotting using a western blot assay for total CREB (tCREB), pCREB, and [alpha]-tubulin in the brain structures. (thefreedictionary.com)
  • We screened duplication mutants for circadian behavioural phenotypes in Drosophila and identified a role for the transcription co-activator CREB-binding protein (CBP) in the circadian clock, as a co-activator of CLK/CYC-dependent transcription. (biomedcentral.com)