CREB-Binding Protein: A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.Cyclic AMP Response Element-Binding Protein: A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Nuclear Proteins: Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.p300-CBP Transcription Factors: A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.Histone Acetyltransferases: Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.E1A-Associated p300 Protein: A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.Rubinstein-Taybi Syndrome: A chromosomal disorder characterized by MENTAL RETARDATION, broad thumbs, webbing of fingers and toes, beaked nose, short upper lip, pouting lower lip, agenesis of corpus callosum, large foramen magnum, keloid formation, pulmonary stenosis, vertebral anomalies, chest wall anomalies, sleep apnea, and megacolon. The disease has an autosomal dominant pattern of inheritance and is associated with deletions of the short arm of chromosome 16 (16p13.3).Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Transcriptional Activation: Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Acetyltransferases: Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.Acetylation: Formation of an acetyl derivative. (Stedman, 25th ed)Nuclear Receptor Coactivator 1: A nuclear receptor coactivator with specificity for ESTROGEN RECEPTORS; PROGESTERONE RECEPTORS; and THYROID HORMONE RECEPTORS. It contains a histone acetyltransferase activity that may play a role in the transcriptional activation of chromatin regions.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Nuclear Receptor Coactivator 3: A nuclear receptor coactivator with specificity for ESTROGEN RECEPTORS and PROGESTERONE RECEPTORS. It contains a histone acetyltransferase activity that may play a role in CHROMATIN REMODELING during the process of nuclear receptor-induced transcription. The coactivator has been found at elevated levels in certain HORMONE-DEPENDENT NEOPLASMS such as those found in BREAST CANCER.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Histones: Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Poly(A)-Binding Proteins: Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.Genes, Reporter: Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Activating Transcription Factor 1: An activating transcription factor that regulates expression of a variety of genes including C-JUN GENES and TRANSFORMING GROWTH FACTOR BETA2.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Gene Products, tax: Transcriptional trans-acting proteins of the promoter elements found in the long terminal repeats (LTR) of HUMAN T-LYMPHOTROPIC VIRUS 1 and HUMAN T-LYMPHOTROPIC VIRUS 2. The tax (trans-activator x; x is undefined) proteins act by binding to enhancer elements in the LTR.Activating Transcription Factors: Activating transcription factors were originally identified as DNA-BINDING PROTEINS that interact with early promoters from ADENOVIRUSES. They are a family of basic leucine zipper transcription factors that bind to the consensus site TGACGTCA of the cyclic AMP response element, and are closely related to CYCLIC AMP-RESPONSIVE DNA-BINDING PROTEIN.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Carrier Proteins: Transport proteins that carry specific substances in the blood or across cell membranes.Cyclic AMP-Dependent Protein Kinases: A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.Response Elements: Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.Human T-lymphotropic virus 1: A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).Tacrolimus Binding Proteins: A family of immunophilin proteins that bind to the immunosuppressive drugs TACROLIMUS (also known as FK506) and SIROLIMUS. EC 5.2.1.-RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Poly(A)-Binding Protein I: A poly(A) binding protein that has a variety of functions such as mRNA stabilization and protection of RNA from nuclease activity. Although poly(A) binding protein I is considered a major cytoplasmic RNA-binding protein it is also found in the CELL NUCLEUS and may be involved in transport of mRNP particles.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Insulin-Like Growth Factor Binding Proteins: A family of soluble proteins that bind insulin-like growth factors and modulate their biological actions at the cellular level. (Int J Gynaecol Obstet 1992;39(1):3-9)Chromatin Immunoprecipitation: A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Luciferases: Enzymes that oxidize certain LUMINESCENT AGENTS to emit light (PHYSICAL LUMINESCENCE). The luciferases from different organisms have evolved differently so have different structures and substrates.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.RNA-Binding Proteins: Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Fatty Acid-Binding Proteins: Intracellular proteins that reversibly bind hydrophobic ligands including: saturated and unsaturated FATTY ACIDS; EICOSANOIDS; and RETINOIDS. They are considered a highly conserved and ubiquitously expressed family of proteins that may play a role in the metabolism of LIPIDS.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Poly(A)-Binding Protein II: A poly(A) binding protein that is involved in promoting the extension of the poly A tails of MRNA. The protein requires a minimum of ten ADENOSINE nucleotides in order for binding to mRNA. Once bound it works in conjunction with CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR to stimulate the rate of poly A synthesis by POLY A POLYMERASE. Once poly-A tails reach around 250 nucleotides in length poly(A) binding protein II no longer stimulates POLYADENYLATION. Mutations within a GCG repeat region in the gene for poly(A) binding protein II have been shown to cause the disease MUSCULAR DYSTROPHY, OCULOPHARYNGEAL.Insulin-Like Growth Factor Binding Protein 3: One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.Periplasmic Binding Proteins: Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.Tacrolimus Binding Protein 1A: A 12-KDa tacrolimus binding protein that is found associated with and may modulate the function of calcium release channels. It is a peptidyl-prolyl cis/trans isomerase which is inhibited by both tacrolimus (commonly called FK506) and SIROLIMUS.Latent TGF-beta Binding Proteins: A family of secreted multidomain proteins that were originally identified by their association with the latent form of TRANSFORMING GROWTH FACTORS. They interact with a variety of EXTRACELLULAR MATRIX PROTEINS and may play a role in the regulation of TGB-beta bioavailability.Cell Line, Tumor: A cell line derived from cultured tumor cells.Insulin-Like Growth Factor Binding Protein 2: One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.

The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus. (1/1067)

Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding protein (CBP) and p300. A stable complex containing both Zta and CBP could be isolated from lytically stimulated, but not latently infected RAJI nuclear extracts. Zta-mediated viral reactivation and transcriptional activation were both significantly inhibited by coexpression of the E1A 12S protein but not by an N-terminal deletion mutation of E1A (E1ADelta2-36), which fails to bind CBP. Zta bound directly to two related cysteine- and histidine-rich domains of CBP, referred to as C/H1 and C/H3. These domains both interacted specifically with the transcriptional activation domain of Zta in an electrophoretic mobility shift assay. Interestingly, we found that the C/H3 domain was a potent dominant negative inhibitor of Zta transcriptional activation function. In contrast, an amino-terminal fragment containing the C/H1 domain was sufficient for coactivation of Zta transcription and viral reactivation function. Thus, CBP can stimulate the transcription of latent EBV in a histone acetyltransferase-independent manner mediated by the CBP amino-terminal C/H1-containing domain. We propose that CBP may regulate aspects of EBV latency and reactivation by integrating cellular signals mediated by competitive interactions between C/H1, C/H3, and the Zta activation domain.  (+info)

The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (2/1067)

Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha-stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha-inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness.  (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (3/1067)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP.  (+info)

Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP. (4/1067)

Yeast two-hybrid system was employed to isolate novel proteins that physically interact with PU.1, a member of Ets family transcription factors. Sequence analyses of several isolated clones positive for beta-galactosidase activity revealed that one of these clones was confirmed to encode a transcriptional coactivator, CREB binding protein (CBP). GST binding assay showed that the interacting sites were located at the transcriptional activation domain of PU.1 through 74-122 and the region spanning residues 1283-1915 of CBP. CBP potentiated PU.1-mediated transcription of the reporter gene driven by the multimerized PU.1-binding sites, suggesting that CBP functions as a coactivator for PU.1. Considering that CBP is a limited cellular component to function as a coactivator for several transcription factors, CBP may mediate synergistic and antagonistic interactions between PU.1 and other transcription factors during the process of hematopoietic cell differentiation.  (+info)

c-Jun functions as a calcium-regulated transcriptional activator in the absence of JNK/SAPK1 activation. (5/1067)

Calcium is the principal second messenger in the control of gene expression by electrical activity in neurons. Recruitment of the coactivator CREB-binding protein, CBP, by the prototypical calcium-responsive transcription factor, CREB and stimulation of CBP activity by nuclear calcium signals is one mechanism through which calcium influx into excitable cells activates gene expression. Here we show that another CBP-interacting transcription factor, c-Jun, can mediate transcriptional activation upon activation of L-type voltage-gated calcium channels. Calcium-activated transcription mediated by c-Jun functions in the absence of stimulation of the c-Jun N-terminal protein kinase (JNK/SAPK1) signalling pathway and does not require c-Jun amino acid residues Ser63 and Ser73, the two major phosphorylation sites that regulate c-Jun activity in response to stress signals. Similar to CREB-mediated transcription, activation of c-Jun-mediated transcription by calcium signals requires calcium/ calmodulin-dependent protein kinases and is dependent on CBP function. These results identify c-Jun as a calcium-regulated transcriptional activator and suggest that control of coactivator function (i.e. recruitment of CBP and stimulation of CBP activity) is a general mechanism for gene regulation by calcium signals.  (+info)

Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in hormone signaling. (6/1067)

Disaggregation of the spherical nuclear bodies termed promyelocytic (PML) oncogenic domains (PODs) is a characteristic of acute promyelocytic leukemia. Here, we demonstrate that the cAMP enhancer binding protein (CREB)-binding protein (CBP) associates with PML in vitro and is recruited to the PODs in vivo. Through its association with CBP, wild-type PML dramatically stimulates nuclear receptor transcriptional activity. These results demonstrate that a fraction of CBP is compartmentalized to the POD through its association with PML and thus suggest that PML and other POD-associated proteins may play an unexpectedly broad role in aspects of transcriptional regulation and human disease.  (+info)

CREB binding protein coordinates the function of multiple transcription factors including nuclear factor I to regulate phosphoenolpyruvate carboxykinase (GTP) gene transcription. (7/1067)

Nuclear factor I (NFI) binds to a region of the phosphoenolpyruvate carboxykinase (GTP) (PEPCK) gene promoter adjacent to the cAMP regulatory element (CRE) and inhibits the induction of transcription from the gene promoter caused by the catalytic subunit of protein kinase A. In vivo footprinting studies demonstrated that both the CRE and the NFI-binding site are occupied by transcription factors, regardless of the presence of factors that stimulate (dibutyryl cAMP or dexamethasone) or inhibit (insulin) transcription from the PEPCK gene promoter. The NFI effects on transcription from the PEPCK gene promoter were observed even in the absence of the NFI binding site, suggesting the possibility of other weaker binding sites on the promoter or an interaction of NFI with a transcriptional co-activator. A mammalian two-hybrid system was used to demonstrate direct interaction between the transactivation domain of NFI-C and the CREB binding domain of the CREB-binding protein (CBP). Overexpression of a gene fragment encoding the CREB binding domain of CBP stimulates transcription from the PEPCK gene promoter. The inhibitory effect of NFI on transcription of the PEPCK gene induced by the catalytic subunit of protein kinase A appears to be the result of an interaction between NFI and the CREB-binding protein in which NFI competes with CREB for binding to the CREB-binding site on CBP. In contrast, glucocorticoids and thyroid hormone use the steroid hormone receptor binding domain of CBP to stimulate transcription from the PEPCK gene promoter. NFI-A combines with dexamethasone or thyroid hormone in an additive manner to stimulate PEPCK gene transcription. We conclude that CBP coordinates the action of the multiple factors known to control transcription of the PEPCK gene.  (+info)

CREB-binding protein is a transcriptional coactivator for hepatocyte nuclear factor-4 and enhances apolipoprotein gene expression. (8/1067)

Hepatocyte nuclear factor-4 (HNF-4) is a liver-enriched transcription factor that is crucial in the regulation of a large number of genes involved in glucose, cholesterol, and fatty acid metabolism and in determining the hepatic phenotype. We have previously shown that HNF-4 contains transcription activation functions at the N terminus (AF-1) and the C terminus (AF-2) which work synergistically to confer full HNF-4 activity. Here, we show that HNF-4 recruits the CREB-binding protein (CBP) coactivator on promoters of genes that contain functional HNF-4 sites. HNF-4 interacts with the N-terminal region of CBP (amino acids 1-771) and the C-terminal region of CBP (amino acids 1812-2441). The two activating functions of HNF-4, AF-1 and AF-2, interact with the N terminus and the N and C terminus of CBP, respectively. In addition, we show that in contrast to the other nuclear hormone receptors the interaction between HNF-4 and CBP is ligand-independent. Recruitment of CBP by HNF-4 results in an enhancement of the transcriptional activity of the latter. CBP does not activate gene expression in the absence of HNF-4, and dominant negative forms of HNF-4 prevent transcriptional activation by CBP, suggesting that the mere recruitment of CBP by HNF-4 is not sufficient for enhancement of gene expression. These findings demonstrate that CBP acts as a transcriptional coactivator for HNF-4 and provide new insights into the regulatory function of HNF-4.  (+info)

*CREB-binding protein

... , also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene. The CREB protein ... "Histone binding protein RbAp48 interacts with a complex of CREB binding protein and phosphorylated CREB". Molecular and ... CREB-binding protein has been shown to interact with: TF2, AR, AIRE, BRCA1, C-jun, CSK, Ccaat-enhancer-binding proteins, CDX2, ... First isolated as a nuclear protein that binds to cAMP-response element-binding protein (CREB), this gene is now known to play ...

*RELA

Gerritsen ME, Williams AJ, Neish AS, Moore S, Shi Y, Collins T (Apr 1997). "CREB-binding protein/p300 are transcriptional ... Aarnisalo P, Palvimo JJ, Jänne OA (Mar 1998). "CREB-binding protein in androgen receptor-mediated signaling". Proceedings of ... "Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with ... Parry GC, Mackman N (Dec 1997). "Role of cyclic AMP response element-binding protein in cyclic AMP inhibition of NF-kappaB- ...

*P300-CBP coactivator family

... also called EP300 or E1A binding protein p300) CBP (also known as CREB-binding protein or CREBBP) Both p300 and CBP interact ... Vo N, Goodman RH (Apr 2001). "CREB-binding protein and p300 in transcriptional regulation". The Journal of Biological Chemistry ... "Global transcriptional coactivators CREB-binding protein and p300 are highly essential collectively but not individually in ... "Extensive brain hemorrhage and embryonic lethality in a mouse null mutant of CREB-binding protein". Mechanisms of Development. ...

*KIX domain

"MLL and CREB bind cooperatively to the nuclear coactivator CREB-binding protein". Molecular and Cellular Biology. 21 (7): 2249- ... and mutability at the binding interface between the p160 coactivator and CREB-binding protein". Protein Science. 13 (1): 203-10 ... "Cooperativity in transcription factor binding to the coactivator CREB-binding protein (CBP). The mixed lineage leukemia protein ... It was thus first termed CREB-binding domain. However, when it was later discovered that it also binds many other proteins, the ...

*EP300

... "p300/cAMP-response-element-binding-protein ('CREB')-binding protein (CBP) modulates co-operation between myocyte enhancer ... "cAMP-response-element-binding-protein-binding protein (CBP) and p300 are transcriptional co-activators of early growth response ... EP300 is closely related to another gene, CREB binding protein, which is found on human chromosome 16. p300 HAT functions as ... Chen Q, Dowhan DH, Liang D, Moore DD, Overbeek PA (July 2002). "CREB-binding protein/p300 co-activation of crystallin gene ...

*Tony Kouzarides

Wolf, D.; Rodova, M.; Miska, E. A.; Calvet, J. P.; Kouzarides, T. (2002). "Acetylation of beta -Catenin by CREB-binding Protein ... Reid, J. L.; Bannister, A. J.; Zegerman, P.; Martínez-Balbás, M. A.; Kouzarides, T. (1998). "E1A directly binds and regulates ... Kouzarides, T.; Brehm, A.; Miska, E. A.; McCance, D. J.; Reid, J. L.; Bannister, A. J. (1998). "Retinoblastoma protein recruits ... Kouzarides, T.; Brehm, A.; Miska, E. A.; McCance, D. J.; Reid, J. L.; Bannister, A. J. (1998). "Retinoblastoma protein recruits ...

*NFATC4

... has been shown to interact with CREB-binding protein. NFAT GRCh38: Ensembl release 89: ENSG00000100968 - Ensembl, May ... NFATC4 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ... Nuclear factor of activated T-cells, cytoplasmic 4 is a protein that in humans is encoded by the NFATC4 gene. The product of ... Lahti AL, Manninen A, Saksela K (May 2003). "Regulation of T cell activation by HIV-1 accessory proteins: Vpr acts via distinct ...

*PROX1

2002). "CREB-binding protein/p300 co-activation of crystallin gene expression". J. Biol. Chem. 277 (27): 24081-9. doi:10.1074/ ... "CREB-binding protein/p300 co-activation of crystallin gene expression". J. Biol. Chem. United States. 277 (27): 24081-9. doi: ... Prospero homeobox protein 1 is a protein that in humans is encoded by the PROX1 gene. PROX1 has been shown to interact with ... PROX1 protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ...

*NFE2

Hung HL, Kim AY, Hong W, Rakowski C, Blobel GA (Apr 2001). "Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)- ... Hung HL, Kim AY, Hong W, Rakowski C, Blobel GA (2001). "Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)-mediated ... NFE2 has been shown to interact with CREB-binding protein. GRCh38: Ensembl release 89: ENSG00000123405 - Ensembl, May 2017 ... and NF-E2-binding proteins". J. Biol. Chem. 277 (44): 41563-70. doi:10.1074/jbc.M208184200. PMID 12196550. Marini MG, Asunis I ...

*MAF (gene)

Chen Q, Dowhan DH, Liang D, Moore DD, Overbeek PA (Jul 2002). "CREB-binding protein/p300 co-activation of crystallin gene ... Chen Q, Dowhan DH, Liang D, Moore DD, Overbeek PA (Jul 2002). "CREB-binding protein/p300 co-activation of crystallin gene ... MAF protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... Vanita V, Singh D, Robinson PN, Sperling K, Singh JR (Mar 2006). "A novel mutation in the DNA-binding domain of MAF at 16q23.1 ...

*SS18L1

... has been shown to interact with CREB-binding protein. Biochemical pull down assays reveal SS18L1 to interact with ... SS18-like protein 1 is a protein that in humans is encoded by the SS18L1 gene. Synovial sarcomas occur most frequently in the ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ... X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169-76 ...

*KLF4

... has been shown to interact with CREB-binding protein. It was found that the transcription factor Klf4 present at the ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. PMID ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ... are dependent on acidic amino acid residues and protein-protein interaction". Nucleic Acids Research. 28 (5): 1106-13. doi: ...

*Autoimmune regulator

... has been shown to interact with CREB binding protein. List of human clusters of differentiation for a list ... regulator protein has transcriptional transactivating properties and interacts with the common coactivator CREB-binding protein ... regulator protein has transcriptional transactivating properties and interacts with the common coactivator CREB-binding protein ... AIRE protein at the US National Library of Medicine Medical Subject Headings (MeSH) Human AIRE genome location and AIRE gene ...

*EBF1

... has been shown to interact with ZNF423 and CREB binding protein. GRCh38: Ensembl release 89: ENSG00000164330 - Ensembl, ... Transcription factor COE1 is a protein that in humans is encoded by the EBF1 gene. EBF1 stands for Early B-Cell Factor 1. EBF1 ... Tsai RY, Reed RR (Jun 1997). "Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 ... Tsai RY, Reed RR (1997). "Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 to ...

*Mdm2

Jin Y, Zeng SX, Dai MS, Yang XJ, Lu H (August 2002). "MDM2 inhibits PCAF (p300/CREB-binding protein-associated factor)-mediated ... HIPK2 is a protein that regulates Mdm2 in this way. The induction of the p14arf protein, the alternate reading frame product of ... Mouse double minute 2 homolog (MDM2) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by ... Wang P, Gao H, Ni Y, Wang B, Wu Y, Ji L, Qin L, Ma L, Pei G (February 2003). "Beta-arrestin 2 functions as a G-protein-coupled ...

*PCAF

"Transforming growth factor-beta regulates DNA binding activity of transcription factor Fli1 by p300/CREB-binding protein- ... "Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) ... Jin Y, Zeng SX, Dai MS, Yang XJ, Lu H (Aug 2002). "MDM2 inhibits PCAF (p300/CREB-binding protein-associated factor)-mediated ... The protein encoded by the PCAF gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, ...

*Sterol regulatory element-binding protein 2

SREBF2 has been shown to interact with INSIG1 and CREB-binding protein. Sterol regulatory element-binding protein GRCh38: ... Sterol regulatory element-binding protein 2 (SREBP-2) also known as sterol regulatory element binding transcription factor 2 ( ... "SREBP transcriptional activity is mediated through an interaction with the CREB-binding protein". Genes & Development. 10 (22 ... "SREBP transcriptional activity is mediated through an interaction with the CREB-binding protein". Genes & Development. 10 (22 ...

*HOXB7

... has been shown to interact with PBX1 and CREB-binding protein. Homeobox GRCh38: Ensembl release 89: ENSG00000260027 - ... "Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins". Genes Dev. 9 (6): 663-74. ... "Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins". Genes Dev. 9 (6): 663-74. ... This gene is a member of the Antp homeobox family and encodes a protein with a homeobox DNA-binding domain. It is included in a ...

*Androgen receptor

CREB-binding protein, Cyclin D1, Cyclin-dependent kinase 7, DACH1, Death associated protein 6, L-DOPA, EFCAB6, Epidermal growth ... Frønsdal K, Engedal N, Slagsvold T, Saatcioglu F (November 1998). "CREB binding protein is a coactivator for the androgen ... Aarnisalo P, Palvimo JJ, Jänne OA (March 1998). "CREB-binding protein in androgen receptor-mediated signaling". Proceedings of ... primarily targeting the ligand binding domain of the protein, while inhibitors that target the N-terminal domain of the protein ...

*Sterol regulatory element-binding protein 1

CREB-binding protein, DAX1 LMNA, and TWIST2. BHLHE40 BHLHE41 Sterol regulatory element-binding protein GRCh38: Ensembl release ... Sterol regulatory element-binding transcription factor 1 (SREBF1) also known as sterol regulatory element-binding protein 1 ( ... "SREBP transcriptional activity is mediated through an interaction with the CREB-binding protein". Genes & Development. 10 (22 ... DNA binding, and transcriptional activation domains of sterol regulatory element-binding protein-1 (SREBP-1)". The Journal of ...

*RPS6KA3

... has been shown to interact with CREB-binding protein, MAPK1 and PEA15. GRCh38: Ensembl release 89: ENSG00000177189 - ... Du K, Montminy M (December 1998). "CREB is a regulatory target for the protein kinase Akt/PKB". The Journal of Biological ... Paudel HK (November 1997). "Phosphorylation by neuronal cdc2-like protein kinase promotes dimerization of Tau protein in vitro ... Ribosomal protein S6 kinase, 90kDa, polypeptide 3, also known as RPS6KA3, is an enzyme that in humans is encoded by the RPS6KA3 ...

*Protein SET

"Functional interaction between nucleosome assembly proteins and p300/CREB-binding protein family coactivators". Molecular and ... Protein SET is a protein that in humans is encoded by the SET gene. Protein SET has been shown to interact with: Acidic leucine ... a novel protein that binds to the acute undifferentiated leukemia-associated protein SET". European Journal of Biochemistry / ... Qu D, Li Q, Lim HY, Cheung NS, Li R, Wang JH, Qi RZ (Mar 2002). "The protein SET binds the neuronal Cdk5 activator p35nck5a and ...

*POU2F3

A possible role for interactions between Skn-1a and CREB-binding protein/p300". J. Biol. Chem. 276 (35): 33036-44. doi:10.1074/ ... POU domain, class 2, transcription factor 3 is a protein that in humans is encoded by the POU2F3 gene. GRCh38: Ensembl release ... 2001). "The POU domain factor Skin-1a represses the keratin 14 promoter independent of DNA binding. ...

*MAFG

Hung HL, Kim AY, Hong W, Rakowski C, Blobel GA (Apr 2001). "Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)- ... and Bach proteins (BACH1 and BACH2). sMaf homodimers bind to a palindromic DNA sequence called the Maf recognition element ( ... "A set of Hox proteins interact with the Maf oncoprotein to inhibit its DNA binding, transactivation, and transforming ... "Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells". Biophysical Journal. 83 (6): 3570-7. ...

*Sirtuin 2

Shimazu T, Horinouchi S, Yoshida M (Feb 2007). "Multiple histone deacetylases and the CREB-binding protein regulate pre-mRNA 3 ... SIRT2 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin ... The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from ... Studies of this protein have often been divergent, highlighting the dependence of pleiotropic effects of SIRT2 on cellular ...

*NFIA

"CREB binding protein coordinates the function of multiple transcription factors including nuclear factor I to regulate ... proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. ... NFIA protein, human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ... Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.[supplied by ...
Histone lysine acetylation is central to epigenetic control of gene transcription. Bromodomains of chromosomal proteins function as acetyl-lysine (Kac) binding domains. However, how bromodomains recognize site-specific histones remains unanswered. Here, we report three three-dimensional solution structures of the bromodomains of the human transcriptional coactivators CREB-binding protein (CBP) and p300/CBP-associated factor (PCAF) bound to peptides derived from histone acetylation sites at lysines 36 and 9 in H3, and lysine 20 in H4. From structural and biochemical binding analyses, we determine consensus histone recognition by the bromodomains of PCAF and CBP, which represent two different subgroups of the bromodomain family. Through bromodomain residues in the ZA and BC loops, PCAF prefers acetylation sites with a hydrophobic residue at (Kac+2) position and a positively charged or aromatic residue at (Kac+3), whereas CBP favors bulky hydrophobic residues at (Kac+1) and (Kac+2), a positively ...
Mier1 encodes a novel transcriptional regulator and was originally isolated as a fibroblast growth factor early response gene. Two major protein isoforms have been identified, MIER1α and β, which differ in their C-terminal sequence. Previously, we demonstrated that both isoforms recruit histone deacetylase 1 (HDAC1) to repress transcription. To further explore the role of MIER1 in chromatin remodeling, we investigated the functional interaction of MIER1 with the histone acetyltransferase (HAT), Creb-binding protein (CBP). Using GST pull-down assays, we demonstrate that MIER1 interacts with CBP and that this interaction involves the N-terminal half (amino acids 1-283) of MIER1, which includes the acidic activation and ELM2 domains and the C-terminal half (amino acids 1094-2441) of CBP, which includes the bromo-, HAT, C/H3 and glutamine-rich domains. Functional analysis, using HEK293 cells, shows that the CBP bound to MIER1 in vivo has no detectable HAT activity. Histone 4 peptide binding assays
Avots, A., Buttmann, M., Chupvilo, S., Escher, C., Smola, U., Bannister, A.J., Rapp, U.R., Kouzarides, T., Serfling, E. CBP/p300 integrates Raf/Rac-signalling pathways in the transcriptional induction of NF-Atc during T cell activation Immunity 10: 515-524 (1999) Chirmule, N., Avots, A., LakshmiTamma, S.M., Pahwa, S., Serfling, E. CD4-mediated signals induce T cell dysfunction in vivo J. Immunol. 163: 644-649 (1999) Bannert, N., Avots, A., Baier, M., Serfling, E., Kurth, R. GA-binding protein factors, in concert with the coactivator CREB binding protein/p300, control the induction of the interleukin 16 promoter in T lymphocytes Proc. Natl. Acad. Sci. USA 96: 1541-1546 (1999) Chupvilo, S., Zimmer, M., Kerstan, A., Glöckner, J., Avots, A., Escher, C., Fischer, C., Inashkina, I., Jankevics, E., Berberich-Siebelt, F., Schmitt, E., Serfling, E. Alternative polyadenylation events contribute to the induction of NF-ATc in effector T cells Immunity 10: 261-269 (1999) Chupvilo, S., Avots, A., ...
In the present study, we sought to examine the molecular basis for the differential regulation of several members of the IFN-α/β gene family (IFNA and IFNB) by IRF-3 and IRF-7. The IFNB, IFNA1, IFNA2, and RANTES promoters were activated by coexpression of either IRF-3 or IRF-7, whereas the IFNA4, IFNA7, and IFNA14 promoters were exclusively activated by IRF-7 and not by IRF-3. Analysis of protein-DNA interactions revealed that recombinant IRF-3 and IRF-7 selectively bound to different regions of the IFNB promoter; IRF-3 bound preferentially to the PRDIII domain of the IFNB promoter, while IRF-7 interacted exclusively with the PRDI domain. PCR-mediated DNA binding site selection results demonstrated that IRF-3 recognized the IRF consensus element 5′-GAAANNGAAANN-3′. Replacement of a single nucleotide within the GAAA core half-site was sufficient to preclude IRF-3 DNA binding. IRF-7 bound to a related sequence motif but with greater flexibility than IRF-3; a single nucleotide replacement did ...
The major finding in this study is that ethanol induces an increase in gene expression via CREB and PKA. This increase in gene expression requires both PKA and CREB phosphorylation. Although we had previously shown that exposure to ethanol resulted in phosphorylation of CREB in NG108-15 cells, there is accumulating evidence that CREB phosphorylation is not sufficient to regulate gene expression under the control of CREs; activation of the coactivator CREB-binding protein (CBP) and other downstream elements is also required for increases in CRE-mediated gene expression (Montminy, 1997; Cardinaux et al., 2000).Thibault et al. (2000) have reported increases in genes the expression of which is known to be cAMP-dependent. However, ethanol activates many different signal transduction pathways in addition to PKA (Diamond and Gordon, 1997), and most genes have regulatory elements activated or inhibited by all of these pathways. Therefore, the experiments presented here are the first demonstration that ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
We also aim to understand the structure-function relationship of very large ("oversized") proteins, practically neglected from a structural point of view thus far. Structural biology has traditionally addressed the structure of small folded proteins, whereas the field of structural disorder has focused on either fully disordered proteins/regions or short disordered elements that undergo induced folding in the presence of their partner. Here we would like to probe into the structure of the very large transcriptional co-activator CREB-binding protein (CBP), by addressing its structure by means traditionally applied in the case of protein complexes. CBP has about seven domains and disordered linker regions connecting them, the topology of which will be outlined by a combination of high-resolution (NMR, X-ray) and low-resolution (MS, EM, AFM) techniques ...
Cancer, Epidermal Growth Factor, Gene, Growth, Survival, Cell Line, Tumors, Disease, Kinase, Mutation, Patient, Phenotype, Cell, Insulin, Mouse, Acetylation, Binding Protein, Creb-binding Protein, Neuroblastoma, Nuclear Export
It does not matter how the diet is restricted. It can be in terms of carbohydrates,fats or proetins. What matters is the diet should be of low calorie. This brings about a change in the CBP proteins that control the genes related to cellular functions ...
The inappropriate sustained SNA increase in OSA patients likely contributes to hypertension, organ damage, and mortality; however, it is unclear how excessive SNA develops in these patients. Several factors, including obesity and increased carotid body chemoreceptor sensitivity due to intermittent hypoxia, have been considered. Obesity could mechanically obstruct the airway and increase SNA through leptin, insulin, angiotensin, and cytokine actions; however, many OSA patients are not obese (23). Carotid body hypersensitivity as a result of intermittent hypoxia has been confirmed in animal models of OSA. In fact, plasticity of the carotid body glomus cells with long-term sensory facilitation and sensitization have been reported (18, 24) and associated with ROS and NOX2-dependent accumulation of HIF1 and the transcriptional coactivator CREB-binding protein (25). Central neuroplasticity. A provocative possibility for OSA-associated SNA dysfunction is that excessive activation of CNS nuclei induces ...
Rubinstein-Taybi syndrome (RSTS) is an uncommon genetic disorder characterised by a typical facies, small stature, broad angulated thumbs and intellectual impairment. Dental changes are a minor, yet significant component of the condition. Craniofacial growth retardation in RSTS is frequently complicated by unerupted teeth, while dental caries is related to the inherent intellectual deficit. Dental problems necessitate interdisciplinary management in terms of oral surgery, conservative dentistry, periodontics and orthodontics. When affected individuals are unco-operative, certain dental procedures may warrant general anaesthesia. In these instances, dental and medical staff will combine their expertise to enhance the well-being of the patient. In addition, specific dental changes may alert the medical practitioner to the possible diagnosis of RSTS. In this article we document the oro-dental manifestations and review the oro-dental approach in the management of three patients with RSTS. Our experience in
Rubinstein, J. "Broad thumb-hallux (Rubinstein-Taybi) Syndrome 1957-1988". Am J Med Gen Suppl . vol. 6. 1990. pp. 3-16. (An early review of 571 cases, this article provides a detailed description of the physical findings in this syndrome.). Wiley, S, Swayne, S, Rubinstein, J, Lanphear, N, Stevens, C. "Rubinstein-Taybi syndrome medical guidelines". Am J Med Genet. vol. 119A. 2003. pp. 101-110. (This article includes specific surveillance and intervention recommendations compiled by a group of pediatric experts.). Cantani, A, Gagliesi, D. "Rubinstein-Taybi syndrome. Review of 732 cases and analysis of typical traits". Eur Rev Med Pharmacol Sci. vol. 2. 1998. pp. 81-87. (This is an analysis of 732 cases and provides a summary of the physical findings of the syndrome and discusses epidemiology and genetics known at the time of publication.). Roelfsema, J, Peters, D. "Rubinstein-Taybi syndrome: clinical and molecular overview". Expert Rev Mol Med. vol. 9. 2007. pp. 1-15. (This article details the ...
Mutations in the coactivator CREB-binding protein (CBP) are a major cause of the human skeletal dysplasia Rubinstein-Taybi syndrome (RTS); however, the mechanism by which these mutations affect skeletal mineralization and patterning is unknown. Here, we report the identification of 3-phosphoinositide-dependent kinase 1 (PDK1) as a key regulator of CBP activity and demonstrate that its functions map to both osteoprogenitor cells and mature osteoblasts. In osteoblasts, PDK1 activated the CREB/CBP complex, which in turn controlled runt-related transcription factor 2 (RUNX2) activation and expression of bone morphogenetic protein 2 (BMP2). These pathways also operated in vivo, as evidenced by recapitulation of RTS spectrum phenotypes with osteoblast-specific Pdk1 deletion in mice (Pdk1osx mice) and by the genetic interactions observed in mice heterozygous for both osteoblast-specific Pdk1 deletion and either Runx2 or Creb deletion. Finally, treatment of Pdk1osx and Cbp+/- embryos with BMPs in utero ...
hypothetical protein, A306_06942, Anapl_13162, AS27_07110, CBP, CBP/p300, CREB-binding protein, CREB binding protein (Rubinstein-Taybi syndrome), crebbp-a, crebbp-b, D623_10028045, E1A binding protein p300, EP300, H920_13788, hmm291030, KAT3A, M91_18874, MDA_GLEAN10009599, N301_13283, N302_12939, N303_04372, N307_13277, N308_10632, N309_02966, N311_11763, N312_01973, N321_00697, N326_12400, N327_01513, N332_08465, N334_05471, N335_14336, N336_02992, N339_02947, p300, p300/CBP, PAL_GLEAN10011621, RSTS, RTS, UY3_13419, Y1Q_016907, Z169_09090, crebbp ...
TY - JOUR. T1 - Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15. AU - Xie, Fuchun. AU - Fan, Qiuhua. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. PY - 2019/1/1. Y1 - 2019/1/1. N2 - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, we designed an ester prodrug of 666-15 through a long-range O,N-acyl transfer reaction for improved aqueous solubility. Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB.. AB - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, ...
Hop on to get the meaning of p/ CIP acronym / slang / Abbreviation. The Medical & Science Acronym / Slang p/ CIP means... AcronymsAndSlang. The p/ CIP acronym/abbreviation definition. The p/ CIP meaning is p300/ CREB-binding protein (CBP)-interacting protein. The definition of p/ CIP by AcronymAndSlang.com
TY - JOUR. T1 - Nuclear factor-kappaB and cAMP response element binding protein mediate opposite transcriptional effects on the Flk-1/KDR gene promoter.. AU - Illi, B.. AU - Puri, P.. AU - Morgante, L.. AU - Capogrossi, M. C.. AU - Gaetano, C.. PY - 2000/6/23. Y1 - 2000/6/23. N2 - -The vascular endothelial growth factor receptor Flk-1/KDR is highly expressed during development and almost disappears in adult tissues. Despite its biological relevance, little is known about the molecular mechanisms controlling its expression. In the present work, it is shown that cAMP response element binding protein (CREB) and nuclear factor-kappaB (NF-kappaB)-related antigens bind specific sequences in the Flk-1/KDR promoter. Functional studies demonstrate that cAMP represses whereas tumor necrosis factor-alpha, an activator of NF-kappaB, stimulates promoter activity. Histone acetyltransferases (HATs) P/CAF and CBP/p300 together with p65/RelA, the catalytic subunit of NF-kappaB, increase Flk-1/KDR promoter ...
CREBBP is targeted by inactivating mutations in follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL). Here, we provide evidence from transgenic mouse models that Crebbp deletion results in deficits in B-cell development and can cooperate with Bcl2 overexpression to promote B-cell lymphoma. Through transcriptional and epigenetic profiling of these B cells, we found that Crebbp inactivation was associated with broad transcriptional alterations, but no changes in the patterns of histone acetylation at the proximal regulatory regions of these genes. However, B cells with Crebbp inactivation showed high expression of Myc and patterns of altered histone acetylation that were localized to intragenic regions, enriched for Myc DNA binding motifs, and showed Myc binding. Through the analysis of CREBBP mutations from a large cohort of primary human FL and DLBCL, we show a significant difference in the spectrum of CREBBP mutations in these 2 diseases, with higher frequencies of ...
The CREBBP gene is associated with autosomal dominant Rubinstein-Taybi syndrome 1 (RSTS1) (MedGen UID: 48517) and is commonly deleted in the recurrent 16p13.3 microdeletion syndrome (OMIM: 610543), a severe form of RSTS resulting from a contiguous gene deletion involving CREBBP as well as other neighboring genes.
In 1963, Rubinstein and Taybi first described a malformation syndrome characterized by distinctive facies, mental retardation, broad thumbs, and broad great toes as are seen in the images below. {file44122}{file44123}{file44124}Deletions in band 16p13 have been described in association with this disorder, and mutations in the cyclic adenosin...
CREBBP - CREBBP (Myc-DDK-tagged)-Human CREB binding protein (CREBBP), transcript variant 1 available for purchase from OriGene - Your Gene Company.
Sigma-Aldrich offers abstracts and full-text articles by [Q Li, H Peng, H Fan, X Zou, Q Liu, Y Zhang, H Xu, Y Chu, C Wang, K Ayyanathan, F J Rauscher, K Zhang, Z Hou].
The concentration of glucose in the bloodstream is regulated by glucose itself, along with the hormones insulin and glucagon. Glucagon stimulates gluconeogenesis in part by regulating phosphorylation of a transcriptional coactivator known as cyclic adenosine monophosphate response element-binding protein 2 (CRTC2). Dentin et al. (see the Perspective by Birnbaum) found that high concentrations of circulating glucose also regulate CRTC2, but do so through stimulation of the hexosamine biosynthetic pathway and consequent O-linked glycosylation of the same serine residue in CRTC2 that is modified by phosphorylation. Thus, CRTC2 integrates signals from hormones and nutrients and might be a target for efforts to treat abnormalities of glucose homeostasis that are associated with diabetes.. R. Dentin, S. Hedrick, J. Xie, J. Yates, III, M. Montminy, Hepatic glucose sensing via the CREB coactivator CRTC2. Science 319, 1402-1405 (2008). [Abstract] [Full Text]. M. J. Birnbaum, Sweet conundrum. Science 319, ...
Lysine propionylation and butyrylation are protein modifications that were recently identified in histones. The molecular components involved in the two protein modification pathways are unknown, hindering further functional studies. Here we report identification of the first three in vivo non-histone protein substrates of lysine propionylation in eukaryotic cells: p53, p300, and CREB-binding protein. We used mass spectrometry to map lysine propionylation sites within these three proteins. We also identified the first two in vivo eukaryotic lysine propionyltransferases, p300 and CREB-binding protein, and the first eukaryotic depropionylase, Sirt1. p300 was able to perform autopropionylation on lysine residues in cells. Our results suggest that lysine propionylation, like lysine acetylation, is a dynamic and regulatory post-translational modification. Based on these observations, it appears that some enzymes are common to the lysine propionylation and lysine acetylation regulatory pathways. Our ...
In type 2 diabetes, chronic hyperglycemia is detrimental to beta-cells, causing apoptosis and impaired insulin secretion. The transcription factor cAMP-responsive element-binding protein (CREB) is crucial for beta-cell survival and function. We inves
TY - JOUR. T1 - Design, synthesis and biological evaluation of regioisomers of 666-15 as inhibitors of CREB-mediated gene transcription. AU - Xie, Fuchun. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. PY - 2016/11/3. Y1 - 2016/11/3. N2 - cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the pathogenesis and maintenance of various types of human cancers. Identification of small molecule inhibitors of CREB-mediated gene transcription has been pursued as a novel strategy for developing cancer therapeutics. We recently discovered a potent and cell-permeable CREB inhibitor called 666-15. 666-15 is a bisnaphthamide and has been shown to possess efficacious anti-breast cancer activity without toxicity in vivo. In this study, we designed and synthesized a series of analogs of 666-15 to probe the importance of regiochemistry in naphthalene ring B. Biological evaluations of these analogs demonstrated that the substitution pattern of the alkoxy and ...
Extensive evidence implicates CREB-dependent gene transcription in memory (Bourtchuladze et al., 1994; Yin et al., 1994; Guzowski and McGaugh, 1997; Bartsch et al., 1998; Kida et al., 2002; Pittenger et al., 2002; Frankland et al., 2004). Multiple signaling pathways phosphorylate CREB at Ser133 (Shaywitz and Greenberg, 1999; Mayr and Montminy, 2001; Lonze and Ginty, 2002), which stimulates the recruitment of coactivators CBP/p300 (Chrivia et al., 1993; Parker et al., 1996). However, this phosphorylation event is not always sufficient to activate transcription (Impey et al., 1996; Mayr and Montminy, 2001) suggesting that CREB-mediated transcription is regulated by additional mechanisms.. In 2003, two laboratories identified a new family of CREB-specific coactivators, now referred to as CRTCs (Iourgenko et al., 2003; Conkright et al., 2003b). CRTC is thought to enhance transcription by facilitating the interaction of CREB with the RNA polymerase II pre-initiation complex (Conkright et al., 2003b; ...
The AlphaLISA SureFire Ultra p-CREB (Ser133) assay kit (High Volume) is an immunoassay for quantitative detection of phospho-CREB in cellular lysates.
I remember the fateful day when I saw my first Kix Commercial with its slogan Kid Tested, Mother Approved. In my ten-year-old mind, Id finally found the loophole in the cruel system of parental cereal control. Somehow, my mom actually obeyed this mantra. Kix occasionally landed in our cabinets, a brightly colored beacon in a sea of white and brown boxes. Recently, I bought a box to see if they were still as great as I remembered.
Looking for online definition of cAMP-responsive element-binding protein 3-like protein 4 in the Medical Dictionary? cAMP-responsive element-binding protein 3-like protein 4 explanation free. What is cAMP-responsive element-binding protein 3-like protein 4? Meaning of cAMP-responsive element-binding protein 3-like protein 4 medical term. What does cAMP-responsive element-binding protein 3-like protein 4 mean?
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BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
TY - JOUR. T1 - The transcriptional coactivator and acetyltransferase p300 in fibroblast biology and fibrosis. AU - Ghosh, Asish K.. AU - Varga, John. PY - 2007/12/1. Y1 - 2007/12/1. N2 - The transcriptional coactivator p300 is a ubiquitous nuclear phosphoprotein and transcriptional cofactor with intrinsic acetyltransferase activity. p300 controls the expression of numerous genes in cell-type and signal-specific manner, and plays a pivotal role in cellular proliferation, apoptosis, and embryogenesis. By catalyzing acetylation of histones and transcription factors, p300 plays a significant role in epigenetic regulation. Recent evidence suggests that abnormal p300 function is associated with deregulated target gene expression, and is implicated in inflammation, cancer, cardiac hypertrophy, and genetic disorders such as the Rubinstein-Taybi syndrome. The activity of p300 is regulated at multiple levels, including developmental stage-specific expression, post-translational modifications, subcellular ...
RefSeq Summary (NM_001675): This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011 ...
Incubation of 3T3-L1 preadipocytes with isobutylmethylxanthine (IBMX), dexamethasone, and insulin, alone or in combination, demonstrated that IBMX, which increased cAMP-response element-binding protein (CREB) phosphorylation, was the predominant regulator of Pde3b expression. Real time PCR and immunoblotting indicated that in 3T3-L1 preadipocytes, IBMX-stimulated induction of Pde3b mRNA and protein was markedly inhibited by dominant-negative CREB proteins. By transfecting preadipocytes, differentiating preadipocytes, and HEK293A cells with luciferase reporter vectors containing different fragments of the 5- flanking region of the Pde3b gene, we identified a distal promoter that contained canonical cis-acting cAMP-response elements (CRE) and a proximal, GC-rich promoter region, which contained atypical CRE. Mutation of the CRE sequences dramatically reduced distal promoter activity; H89 inhibited IBMX-stimulated CREB phosphorylation and proximal and distal promoter activities. Distal promoter ...
Chromosomal translocations that fuse the mixed lineage leukemia (MLL) gene with multiple partners typify acute leukemias of infancy as well as therapy-related leukemias. We utilized a conditional knockin strategy to bypass the embryonic lethality caused by MLL-CBP expression and to assess the immediate effects of induced MLL-CBP expression on hematopoiesis. Within days of activating MLL-CBP, the fusion protein selectively expanded granulocyte/macrophage progenitors (GMP) and enhanced their self-renewal/proliferation. MLL-CBP altered the gene expression program of GMP, upregulating a subset of genes including Hox a9. Inhibition of Hox a9 expression by RNA interference demonstrated that MLL-CBP required Hox a9 for its enhanced cell expansion. Following exposure to sublethal γ-irradiation or N-ethyl-N-nitrosourea (ENU), MLL-CBP mice developed myelomonocytic hyperplasia and progressed to fatal myeloproliferative disorders. These represented the spectrum of therapy-induced acute myelomonocytic ...
Expression of CREBBP (CBP, KAT3A, RSTS, RTS) in cervix, uterine tissue. Antibody staining with HPA055861 and CAB004212 in immunohistochemistry.
Here, we present a structural and dynamic description of CBP-ID4 at atomic resolution. ID4 is the fourth intrinsically disordered linker of CREB-binding protein (CBP). In spite of the largely disordered nature of CBP-ID4, NMR chemical shifts and relaxation measurements show a significant degree of α-helix sampling in the protein regions encompassing residues 2-25 and 101-128 (1852-1875 and 1951-1978 in full-length CBP). Proline residues are uniformly distributed along the polypeptide, except for the two α-helical regions, indicating that they play an active role in modulating the structural features of this CBP fragment. The two helical regions are lacking known functional motifs, suggesting that they represent thus-far uncharacterized functional modules of CBP. This work provides insights into the functions of this protein linker that may exploit its plasticity to modulate the relative orientations of neighboring folded domains of CBP and fine-tune its interactions with a multitude of ...
Somatic mutations in CREBBP occur frequently in B-cell lymphoma. Here, we show that loss of CREBBP facilitates the development of germinal center (GC)-derived lymphomas in mice. In both human and murine lymphomas, CREBBP loss-of-function resulted in focal depletion of enhancer H3K27 acetylation and aberrant transcriptional silencing of genes that regulate B-cell signaling and immune responses, including class II MHC. Mechanistically, CREBBP-regulated enhancers are counter-regulated by the BCL6 transcriptional repressor in a complex with SMRT and HDAC3, which we found to bind extensively to MHC class II loci. HDAC3 loss-of-function rescued repression of these enhancers and corresponding genes, including MHC class II, and more profoundly suppressed CREBBP-mutant lymphomas in vitro and in vivo. Hence, CREBBP loss-of-function contributes to lymphomagenesis by enabling unopposed suppression of enhancers by BCL6/SMRT/HDAC3 complexes, suggesting HDAC3-targeted therapy as a precision approach for ...
The activation of the transcription factor NF-kB is central to the control of the cellular response triggered by many stimuli. Once released from the inhibitory molecule IkB, NF-kB is translocated to the nucleus, and it has to be phosphorylated to activate transcription. In protein kinase C (PKC)-deficient cells, NF-kB is transcriptionally inactive and the phosphorylation of the RelA subunit in response to tumor necrosis factor (TNF-alpha) is severely impaired. In vitro assays showed that PKC directly phosphorylates RelA. Here we demonstrate that Ser311 accounts for PKC phosphorylation of RelA and that this site is phosphorylated in vivo in response to TNF-alpha. Also, an inactivating mutation of that residue severely impairs RelA transcriptional activity, blocks its anti-apoptotic function and abrogates the interaction of RelA with the co-activator CBP as well as its recruitment, and that of RNA polymerase II (Pol II) with the interleukin-6 (IL-6) promoter. The interaction of endogenous CBP ...
Freds Dērsts piedzima Džeksonvilā, Floridā, ASV. Fredu audzināja viņa māte Anita, jo viņa bioloģiskais tēvs aizgāja no ģimenes, kad Fredam bija tikai dažas nedēļas. Freds ar māti dzīvoja ļoti trūcīgi, viņiem nebija ne mājas, ne darba, ne naudas. Viņi dzīvoja baznīcas bēniņos, ēdot bērnu pārtiku, ko nesa draudzes locekļi. Kad Fredam bija divi gadi, Anita iepazinās ar policijas oficieri Bilu, ar kuru drīz vien viņa apprecējās.. Fredam jaunībā patika tā pati mūzika, kas viņa vecākiem, un viņš bieži ākstījās dejojot un iztēlojoties, ka uzstājas publikas priekšā. Kad Freds paaugās, viņš un viņa pusbrālis Korijs (Bila un Anitas dēls) kļuva par Kiss faniem.. Vēlāk Freda ģimene pārvācās no Džeksonvillas, Floridā, uz Gastoniju, Ziemeļkarolīnā, kur Dērsts mācījās Hunter Huss vidusskolā.[1] Šeit viņš sāk aizrauties ar hiphopu un izveido breika dejošanas grupu Reckless Crew. Viņš sāk interesēties par kultūru, kas ir ...
Vislielāko vīrusu daudzumu inficētie pacienti izdala 2.-3. slimības dienā, t.i., šajā periodā viņi ir "visinfekciozākie", tomēr nelielos, inficēšanai pietiekamos daudzumos vīrusa izdale var saglabāties līdz pat 1-2 nedēļām.. Attiecībā uz sabiedrībā valdošo uzskatu, ka gaisa recirkulācijas sistēmas, piemēram, lidmašīnās, paaugstina saaukstēšanās risku, ASV 2002. gadā veiktais pētījums šādu faktu nav apstiprinājis, tāpēc šobrīd jādomā, ka pārneses ceļam ar nemazgātām rokām ir vislielākā nozīme saaukstēšanās vīrusu izplatībā.. Nereti tiek diskutēts par to, cik bieži gada laikā slimot ar saaukstēšanos ir "normāli". Vidēji pirmsskolas vecuma bērni slimo 6-8 (līdz pat 12) reizes gadā, katru reizi slimībai ilgstot aptuveni 14 dienas, savukārt vecāki bērni un pieaugušie vidēji slimo 2-4 reizes gadā, katru reizi simptomiem ilgstot 5-7 dienas.. Smēķētājiem gan simptomi parasti ilgst vairāk par nedēļu hroniska elpceļu ...
Rubinstein-Taybi syndrome (RSTS) is a rare condition with a prevalence of 1 in 125,000-720,000 births and characterized by clinical features that include facial, dental, and limb dysmorphology and growth retardation. Most cases of RSTS occur sporadically and are caused by de novo mutations. Cytogenetic or molecular abnormalities are detected in only 55% of RSTS cases. Previous genetic studies have yielded inconsistent results due to the variety of methods used for genetic analysis. The purpose of this study was to use whole exome sequencing (WES) to evaluate the genetic causes of RSTS in a young girl presenting with an Autism phenotype. We used the Autism diagnostic observation schedule (ADOS) and Autism diagnostic interview revised (ADI-R) to confirm her diagnosis of Autism. In addition, various questionnaires were used to evaluate other psychiatric features. We used WES to analyze the DNA sequences of the patient and her parents and to search for de novo variants. The patient showed all the typical
One of the major functions of the metal response element-binding transcription factor 1(MTF-1) is to sense and maintain sub-nanomolar to nanomolar zinc levels in response to influxes of labile zinc within the cell. MTF-1 responses to elevated zinc include up regulation of metallothionein (MT-I & II) and efflux transporter (ZnT1) genes. MTF-1 also responds to oxidative stress and heavy metal loads. Due to a lack of liver development, MTF-1 is essential for embryogenesis as determined from knockout mice. The zinc dependence of DNA-binding and interactions with other transcription factors has been identified as major determinants in the homeostatic regulation of labile intracellular zinc by MTF-1. p300 along with its paralog, cyclic-AMP response element binding protein (CBP), have histone acetyltransferase protein scaffold functions and interact with other transcription factors. Previous studies have shown that p300, Sp1 and MTF-1 form a complex. It was also found that a zinc dependent interaction ...
A total of 82 genes have now been located on chromosome 16, with 8 new assignments since HGM 11. Three new disease-gene localizations were reported at the workshop.. Two patients with Rubinstein-Taybi syndrome (dysmorphic facies, broad thumbs, big toes, and mental retardation) were reported to have a reciprocal translocation involving the short arm of chromosome 16. Martijn Breuning (Leiden University, Netherlands) reported that 6 of 24 patients with this syndrome were found by FISH to have submicroscopic deletions.. The second disease localization was reported by Dan Kastner (NIH, Bethesda). Familial Mediterranean Fever, an autosomal recessive disorder characterized by acute attacks of fever with sterile peritonitis, pleurisy, or synovitis, was genetically mapped to the chromosome 16 short arm. Linkage disequilibrium between different ethnic groups strongly suggests the presence of at least two mutant alleles with different clinical manifestations.. The third new disease assignment was a gene ...
Centromere protein J is a protein that in humans is encoded by the CENPJ gene. It is also known as centrosomal P4.1-associated protein (CPAP). During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with Seckel syndrome and primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and mental retardation. The Drosophila ortholog, sas-4, has been shown to be a scaffold for a cytoplasmic complex of Cnn, Asl, CP-190, tubulin and D-PLP (similar to the human proteins PCNT and AKAP9). These complexes are then anchored at the centriole to begin formation of the ...
Expression of CREBBP (CBP, KAT3A, RSTS, RTS) in pancreas tissue. Antibody staining with HPA055861 and CAB004212 in immunohistochemistry.
The cellular transcription factor CREB (cAMP response element-binding protein) helps learning and the stabilization and retrieval of fear-based, long-term memories. This is done mainly through its expression in the hippocampus and the amygdala. Studies supporting the role of CREB in cognition include those that knock out the gene, reduce its expression, or overexpress it. Research suggests that CREB has a role in the molecular steps that stabilize memory in the brain, including that of emotional memory. Evidence of CREBs role in emotional memory falls into three experimental categories: negative manipulations (where the levels of CREB were lowered), positive manipulations (where the levels of CREB were increased), and non-interventions (where the endogenous levels of CREB were tracked before and after learning). Knockout studies in Aplysia sea slugs indicated that decreasing CREB function blocks long-term changes in synaptic function, but not short-term ones. Changes in synaptic function (i.e., ...
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TY - JOUR. T1 - CREB activation in the rapid, intermediate, and delayed ischemic preconditioning against hypoxic-ischemia in neonatal rat. AU - Lin, Wan Ying. AU - Chang, Ying Chao. AU - Lee, Hsueh Te. AU - Huang, Chao Ching. PY - 2009/2. Y1 - 2009/2. N2 - Ischemic preconditioning (IP) is a defense program in which exposure to sublethal ischemia followed by a period of reperfusion results in subsequent resistance to severe ischemic insults. Very few in vivo IP models have been established for neonatal brain. We examined whether rapid, intermediate, and delayed IP against hypoxic-ischemia (HI) could be induced in neonatal brain, and if so, whether the IP involved phosphorylation of cAMP response element-binding protein (pCREB) after HI. Postnatal day 7 rat pups were subjected to HI at 2 h (2-h IP), 6 h (6-h IP), or 22 h (22-h IP) after IP. We found all three IP groups had significantly reduced neuronal damage and TUNEL-(+) cells 24 h post-HI than no-IP group. Compared with control, the no-IP ...
Օրթոֆոսֆորական թթու (ֆոսֆորական թթու), միջին ուժի անօրգանական թթու է, քիմիական բանաձևն է H3PO4, այն ստանդարտ պայմաններում իրենից ներկայացնում է անգույն հիդրոսկոպիկ բյուրեղներ։ 213 °C ջերմաստրճանից բարձր ջերմաստիճանում փոխակերպվում է պիրոֆոսֆորական թթվի H4P2O7։ Ջրում լավ լուծելի է։ Հիմնականում օրթոֆոսֆորական թթու անվանում են 85%-անոց ջրային լուծույթը(անգույն, անհոտ հեղուկ է)։ Լուծվում է նաև էթանոլում և այլ լուծիչներում։ ...
We have shown previously that chronic administration of adrenocorticotropic hormone (ACTH) causes a significant decrease in hippocampal cell proliferation and neurogenesis. This effect in rats treated chronically with ACTH was not influenced by the chronic administration of imipramine, but was reversed by coadministration of imipramine and lithium. The present study was undertaken to further characterize the mechanism underlying the effect of imipramine and lithium on hippocampal cell proliferation and neurogenesis, by investigating the effects of treatment on the expression of brain-derived neurotrophic factor (BDNF), total cyclic adenosine monophosphate response element-binding protein (CREB), and phosphorylated CREB (pCREB) of the CREB signaling system, as well as Wnt 3a and cyclin D1 of the Wnt signaling pathway in the hippocampus of saline- and ACTH-treated rats. ACTH treatment significantly decreased the expression of cyclin D1. Treatment with imipramine and lithium increased the expression of
TY - JOUR. T1 - Specific role for p300/CREB-binding protein-associated factor activity in E2F1 stabilization in response to DNA damage. AU - Ianari, Alessandra. AU - Gallo, Rita. AU - Palma, Marzia. AU - Alesse, Edoardo. AU - Gulino, Alberto. PY - 2004/7/16. Y1 - 2004/7/16. N2 - E2F1, a member of the E2F family of transcription factors, plays a pivotal role in controlling both physiological cell-cycle progression and apoptotic cell death in response to DNA damage and oncogene activation. In response to genotoxic stresses, E2F1 is stabilized by signals that include ATM-dependent phosphorylation. We recently demonstrated that DNA damage induces also E2F1 acetylation, which is required for its recruitment onto apoptotic gene promoters. Here we show that E2F1 is stabilized in response to doxorubicin and cisplatin treatments even in the absence of either ATM-dependent phosphorylation or p53 and cAbl, two major transducers of DNA damage signaling. We found that acetylation of E2F1 is, instead, ...
J Immunol. 2001 Oct 1;167(7):3626-34. Harton JA, Zika E, Ting JP. The histone acetyltransferase domains of CREB-binding protein (CBP) and p300/CBP-associated factor are not necessary for cooperativity with the class II transactivator. J Biol Chem. 2001 Oct 19;276(42):38715-20. Deffrennes V, Vedrenne J, Stolzenberg MC, Piskurich J, Barbieri G, Ting JP, Charron D, Alcaide-Loridan C. Constitutive expression of MHC class II genes in melanoma cell lines results from the transcription of class II transactivator abnormally initiated from its B cell-specific promoter. J Immunol. 2001 Jul 1;167(1):98-106. Li G, Harton JA, Zhu X, Ting JP. Downregulation of CIITA function by protein kinase a (PKA)-mediated phosphorylation: mechanism of prostaglandin E, cyclic AMP, and PKA inhibition of class II major histocompatibility complex expression in monocytic lines. Mol Cell Biol. 2001 Jul;21(14):4626-35. Linhoff MW, Harton JA, Cressman DE, Martin BK, Ting JP. Two distinct domains within CIITA mediate ...
The present report details genotype and phenotype of nine patients with an interstitial 16p13.3 duplication. Three patients that were previously reported were also included,5-7 with more precise genotypic and phenotypic data for one of these (patient 2).6 Careful assessment of the phenotypic features of these individuals enabled the description of a characteristic phenotype, with normal to moderately retarded mental development, mild arthrogryposis-like anomalies of the musculoskeletal system (club feet, congenital hip dislocation, or camptodactyly of the fingers and toes), mild facial dysmorphism that changes with age and occasional anomalies of the heart (atrial septal defect, tetralogy of Fallot).. It is noteworthy that all patients were selected for study because of MR and/or congenital anomalies. There might thus be-as in most genetic disorders-a bias towards the more severe end of the spectrum in the described phenotype; normal or mildly affected individuals do not present at the genetics ...
The optical constraints of the adult Drosophila compound eye require that during development every cell must make the appropriate cell fate choice and position itself correctly within the growing retinal lattice. Early models predicted that each cell would express an "individualized" set of membrane-bound receptors and specific DNA-binding transcription factors, which would then be linked to the basal transcriptional machinery by yet another set of "personalized" bridging molecules. However, experimental evidence points to a much more complicated mechanism for producing the fly eye. It is clear that a cell within the developing eye will be presented with many extracellular signals and will express several receptors along with overlapping sets of transcription factors. How a cell sorts through this information and ultimately makes the correct choice is a problem that is not restricted to the insect eye but rather is a common theme in metazoan development. The fly eye has proven to be a tractable ...
CG-001 is a selective Wnt/β-catenin signalling inhibitor with an IC50 of 3μM. ICG 001, a small molecule that down-regulates beta-catenin/T cell factor signaling by specifically binding to cyclic AMP response element-binding protein. ICG001 selectively ind
TY - JOUR. T1 - Epistatic interaction of CREB1 and KCNJ6 on rumination and negative emotionality. AU - Lazary, Judit. AU - Juhasz, Gabriella. AU - Anderson, Ian M.. AU - Jacob, Christian P.. AU - Nguyen, T. Trang. AU - Lesch, Klaus Peter. AU - Reif, Andreas. AU - Deakin, J. F.William. AU - Bagdy, Gyorgy. PY - 2011/1/1. Y1 - 2011/1/1. N2 - G protein-activated K+ channel 2 (GIRK2) and cAMP-response element binding protein (CREB1) are involved in synaptic plasticity and their genes have been implicated depression and memory processing. Excessive rumination is a core cognitive feature of depression which is also present in remission. High scores on the Ruminative Response Scale (RRS) questionnaire are predictive of relapse and recurrence. Since rumination involves memory, we tested the hypothesis that variation in the genes encoding GIRK2 (KCNJ6) and CREB1 mechanisms would influence RRS scores. GIRK2 and CREB1 polymorphisms were studied in two independent samples (n = 651 and n = 1174) from the ...
SMCA4_HUMAN] Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific ...
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Will I eat the Joel Fuhrman way everyday? Probably not. Okay, definitely not. But Ill try to work some of his principles in (especially that part about more veggies) where I can. And Im thinking I might do a whole Eat to Live cleanse in January, but only for 30 days. I cant live without Gardein for THAT long, after all. If I had cancer or some life-threatening disease or if I was overweight, I might consider adopting this diet for real-real. But Im pretty healthy just the way I am now. But its nice to know I wont have to make too many changes to adopt a healthier diet ...
TransAM CREB and TransAM pCREB Kits are DNA-binding ELISAs that quanify the activated transcription factors using a method that is faster and more sensitive than gelshift, without radioactivity and gels.
Ca2+ release induced by depolarization of skeletal muscle cells can be separated into two independent components; the fast Ca2+ transient, homogenously distributed through the cell that corresponds to excitation-contraction coupling, and the slow Ca2+ transient, with a distinct nuclear component, generated by IP3 (Jaimovich et al., 2000). Slow Ca2+ signals with similar characteristics, although displaying particular kinetics can also be elicited by androgens like testosterone (Estrada et al., 2000; Estrada et al., 2003), or hormones such as insulin-like growth factor (Espinosa et al., 2004). Slow Ca2+ signals have been shown to participate in phosphorylation of both MAP kinases ERK 1 and 2, and transcription factor CREB, as well as in the expression of early genes fos, jun and egr-1 (Powell et al., 2001; Carrasco et al., 2003). In this work, we determined that type 1 and 3 IP3Rs are expressed and are functional in myonuclei, mediating nucleoplasmic Ca2+ changes, which in turn induce CREB ...
Co-localization of p-CREB and GFAP in coronal brain sections of ACC. Photomicrographs showed the expression of p-CREB (red) and GFAP (an astrocytic marker, gree
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p300 and its family member, CREB-binding protein (CBP), function as key transcriptional coactivators by virtue of their interaction with the activated forms of certain transcription factors. In a search for additional cellular targets of p300/CBP, a protein-protein cloning strategy, surprisingly identified SRC-1, a coactivator involved in nuclear hormone receptor transcriptional activity, as a p300/CBP interactive protein. p300 and SRC-1 interact, specifically, in vitro and they also form complexes in vivo. Moreover, we show that SRC-1 encodes a new member of the basic helix-loop-helix-PAS domain family and that it physically interacts with the retinoic acid receptor in response to hormone binding. Together, these results implicate p300 as a component of the retinoic acid signaling pathway, operating, in part, through specific interaction with a nuclear hormone receptor coactivator, SRC-1.. ...
The formation of complex ectodermal organs begins with multipotent stem cells that undergo many basic cellular events. During the formation of a complex organ, there are many factors that need to be considered such as patterning, size, and shape in order to maintain proper organ function. Feather development is a good model. The feather field must be patterned to establish how many feathers, the size must be determined, and the shape of the feather must be appropriate for its function. Canonical and non-canonical Wnt signaling has been implicated in many crucial steps in feather bud development.; Canonical Wnt signaling involves the stabilization and accumulation of beta-catenin, which is subsequently translocated to the nucleus. There, beta-catenin interacts with various coactivators including CREB-binding protein (CBP) and p300, which results in the expression of different genes downstream of beta-catenin/TCF that may direct cells towards a path of pluripotency or differentiation. One of the ...
Mucoepidermoid carcinoma (MEC) arises from multiple organs and accounts for the most common types of salivary gland malignancies. Currently, patients with unresectable and metastatic MEC have poor long-term clinical outcomes and no targeted therapies are available. The majority of MEC tumors contain a t(11;19) chromosomal translocation that fuses two genes, CRTC1 and MAML2, to generate the chimeric protein CRTC1-MAML2. CRTC1-MAML2 displays transforming activity in vitro and is required for human MEC cell growth and survival, partially due to its ability to constitutively activate CREB-mediated transcription. Consequently, CRTC1-MAML2 is implicated as a major etiologic molecular event and a therapeutic target for MEC. However, the molecular mechanisms underlying CRTC1-MAML2 oncogenic action in MEC have not yet been systematically analyzed. Elucidation of the CRTC1-MAML2-regulated transcriptional program and its underlying mechanisms will provide important insights into MEC pathogenesis that are essential
Peptides , Signal Transduction Peptides , Steroid Receptor Coactivator-1, SRC-1 (686-700); This peptide is amino acids 686 to 700 fragment containing the second LXXLL motif, derived from NR box II of steroid receptor coactivator (SRC1). Coactivator proteins interact with nuclear receptors in a ligand-dependent manner and augment transcription.; RHKILHRLLQEGSPS; H-Arg-His-Lys-Ile-Leu-His-Arg-Leu-Leu-Gln-Glu-Gly-Ser-Pro-Ser-OH
Defects in gene transcription and mitochondrial function have been implicated in the dominant disease process that leads to the loss of striatal neurons in Huntingtons disease (HD). Here we have used precise genetic HD mouse and striatal cell models to investigate the hypothesis that decreased cAMP responsive element (CRE)-mediated gene transcription may reflect impaired energy metabolism. We found that reduced CRE-signaling in HdhQ111 striatum, monitored by brain derived neurotrophic factor and phospho-CRE binding protein (CREB), predated inclusion formation. Furthermore, cAMP levels in HdhQ111 striatum declined from an early age (10 weeks), and cAMP was significantly decreased in HD postmortem brain and lymphoblastoid cells, attesting to a chronic deficit in man. Reduced CRE-signaling in cultured STHdhQ111 striatal cells was associated with cytosolic CREB binding protein that mirrored diminished cAMP synthesis. Moreover, mutant cells exhibited mitochondrial respiratory chain impairment, ...
Our hypothesis is based on several lines of evidence. First, the effects of cAMP and DA on both 4xCRE (Figs. 3a,b, 5) and c-fos and BDNF mRNA expression (Fig. 6) are blocked by NMDAR antagonists. Second, two structurally distinct inhibitors of neuronal EAA uptake, TBOA (Fig. 7) and trans-PDC (supplemental Fig. S3, available at www.jneurosci.org as supplemental material), potentiated the stimulation of gene transcription by cAMP. Third, the aspartate+glutamate-, but not the glutamate-only-, scavenging system abolished stimulation of CREB-dependent gene transcription by forskolin (Fig. 8); the aspartate-scavenging enzyme, GOT, degrades l- but not d-aspartate demonstrating that l-aspartate is the active extracellular EAA in this signaling pathway. Finally, forskolin was found to induce release of aspartate but not glutamate (Fig. 9). Together, these results lead to the conclusion that cAMP-induced release of aspartate and the resulting activation of NR2B-containing NMDARs mediate the effects of ...
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor ...
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Targeting hepatic CREB to reduce glucose output as a treatment option for diabetes is a highly controversial notion. Inhibition of CREB activity in the liver us...
A-D: Phospho-CREB-positive cells are increased after beraprost treatment. (7A and 7C). Representative images of brain sections show p-CREB immunostaining in Y
The interferon regulatory factor (IRF) family of transcription factors control the expression of several interferon genes, and are involved in the JAK-STAT signaling pathway. Interferon regulatory factor-3 (IRF-3) was discovered based on its homology to IRF-1 and IRF-2. IRF3 is produced as an inactive cytoplasmic form that dimerizes and forms a complex with CREB-binding protein, after phosphorylation of Ser-386. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. IRF-3 plays an important role in the innate immune systems response to viral infection. Double-stranded RNA (dsRNA) and toll-like receptor (TLR) signaling can also induce dimerization and phosphorylation of IRF-3. IRF-3 has been shown to induce significant apoptosis in primary macrophages.. ...
The interferon regulatory factor (IRF) family of transcription factors control the expression of several interferon genes, and are involved in the JAK-STAT signaling pathway. Interferon regulatory factor-3 (IRF-3) was discovered based on its homology to IRF-1 and IRF-2. IRF3 is produced as an inactive cytoplasmic form that dimerizes and forms a complex with CREB-binding protein, after phosphorylation of Ser-386. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. IRF-3 plays an important role in the innate immune systems response to viral infection. Double-stranded RNA (dsRNA) and toll-like receptor (TLR) signaling can also induce dimerization and phosphorylation of IRF-3. IRF-3 has been shown to induce significant apoptosis in primary macrophages.. ...
PDZ-LIM proteins form a family of the scaffolding protein essential for both embryonic and post-natal development. ENH1 (PDLIM5) is a PDZ-LIM protein, composed of an N-terminal PDZ domain and 3 LIM domains at the C-terminal end. The enh gene encodes for several splice variants that have opposite functions. ENH1 promotes the cardiomyocytes hypertrophy whereas ENH splice variants lacking LIM domains prevents it. At the molecular level, ENH1 interacts with Protein kinase C (PKC) and Protein Kinase D1 (PKD1) both kinases playing a pivotal role in the pathological remodeling of the heart. In addition, the binding of ENH1s LIM domains to PKC is sufficient to activate the kinase without any stimulation. However, the downstream events of the ENH1-PKC/PKD1 complex remain unknown. PKC and PKD1 are known to phosphorylate the transcription factor cAMP-response element binding protein (CREB) in cardiomyocytes. We therefore hypothesized that ENH1 could be a play a role in the PKC/PKD1-dependent activation of ...
Alzheimers disease (AD) is a progressive neurodegenerative disease and the most common form of senile dementia. Recently, scientists have put significant effort into exploring the molecular mechanisms involved in the pathological processes leading to the disease. A vast number of studies have focused on understanding the nitric oxide (NO) signaling pathway, which culminates with the phosphorylation of the transcription factor cAMP-responsive element-binding protein (CREB) through the increase of the second messenger cyclic guanosine monophosphate (cGMP) and activation of cGMP-dependent protein kinase. This book chapter provides an overview of the progress being made in modulating the hippocampal synaptic transmissions, which are critical for learning and memory, by targeting the different components of the NO/cGMP/CREB phosphorylation signaling pathway. Furthermore, a description of recent research on this pathway through the use of phosphodiesterase inhibitors is emphasized.
LATS1/LATS2 are primarily considered as central players in the Hippo tumour suppressor pathway, however they also participate in a range of other signalling pathways. (Zhao,2010). The core of the Hippo pathway consists of Hpo, Wts, Sav and Mats. Hippo (Hpo) is a Ste20 family protein kinase that complexes with the regulatory scaffold protein Salvador (Sav). The Hpo/Sav complex then phosphorylates and activates Warts (Wts). Wts has an activating subunit Mats. The active Wts/Mats complex then in turn phosphorylates the transcriptional co-activators Yorkie (Yki) in flies, and YAP and TAZ in mammals. Phosphorylation of these transcriptional co-activators leads to their association with 14-3-3 family proteins, cytoplasmic retention and inactivation (Zhao,2007). In the presence of active CK1 kinase, LATS1/2 mediated phosphorylation of YAP also leads to the recruitment of the beta-TRCP SCF E3 ligase thereby triggering the subsequent proteasomal degradation of YAP (Zhao,2010). The LATS kinases prefer to ...
... HOT new cereal coupon from General Mills for a $.75 off any one box of Kix, Kix Berry or Kix Honey brand cereal! Kix cereal printable coupons This coupon is located on my printable coupon page under foods. You can print it twice..... The two I printed had an expiration date 30
The overall goal of this project is to elucidate the molecular basis for how protein acetyltransferases (PATs) recognize and acetylate their cognate protein sub...
The enzyme histone acetyltransferases (HATs) are injected in high amount into a yeast cell. Explain how this might impact DNA packing and/or cellular function.
Youve probably heard that life is short and that things can change at the drop of a hat, but a surprise diagnosis can still come as quite a shock. When Ashley Niels took her 12-year-old dog Spunky to the vet recently, she never expected what they told her after running some tests. googletag.cmd.push(function() { googletag.display(VN_PG_DCBP_ATF); […]. ...
p300 (EP300) and CBP (CREBBP) are transcriptional coactivators with histone acetyltransferase activity. Various beta cell transcription factors can recruit p300/CBP, and thus the coactivators could be important for beta cell function and health in vivo. We hypothesized that p300/CBP contribute to the development and proper function of pancreatic islets. To test this, we bred and studied mice lacking p300/CBP in their islets. Mice lacking either p300 or CBP in islets developed glucose intolerance attributable to impaired insulin secretion, together with reduced alpha and beta cell area and islet insulin content. These phenotypes were exacerbated in mice with only a single copy of p300 or CBP expressed in islets. Removing p300 in pancreatic endocrine progenitors impaired proliferation of neonatal alpha and beta cells. Mice lacking all four copies of p300/CBP in pancreatic endocrine progenitors failed to establish alpha and beta cell mass postnatally. Transcriptomic analyses revealed significant ...
Mitigating Ischemic Injury of Stem Cell-Derived Insulin-Producing Cells after Transplant Scientists showed that more than half of stem cell-derived insulin-producing cells (SCIPCs) die shortly after transplantation. Nutrient deprivation and hypoxia acted synergistically to kill SCIPCs in vitro. Amino acid supplementation rescued SCIPCs from nutrient deprivation, likely by providing cellular energy. [Stem Cell Reports] Full Article , Graphical Abstract Glucose Potentiates β-Cell Function by Inducing Tph1 Expression in Rat Islets Global gene expression patterns revealed that tryptophan hydroxylase 1 (Tph1) was the most profound of genes that are up-regulated in rat islets exposed to high glucose. Calcium and cAMP signals synergistically mediated glucose-stimulated Tph1 transcription in β-cells by activating cAMP-responsive element-binding protein and promoting its binding with a Tph1 promoter. [FASEB J] Abstract Differential Effects of Linagliptin on the Function of Human Islets Isolated from ...
Cell-permeable. A potent, non-competitive inhibitor of p300 and PCAF (p300/CBP-associated factor) histone acetyltransferase (HAT) activities (IC50~8.5 µM and ~5 µM, res
201 Page 210 202 D. 3 KID domain from CREB, phosphorylated on Ser133 Snovitra xl 60 mg in a complex with the KIX domain of CBP. AlвBadr were applied successfully for the determination of miconazole and the other anti- fungal drug in their pharmaceutical formulation. The snovitra and ventricular electrograms are on top and the s novitra markers at snovtira bottom.
GO Terms Descrition:, locomotor rhythm, positive regulation of transcription from RNA polymerase II promoter, R3/R4 cell fate commitment, synapse assembly, compound eye development, hemopoiesis, transcription factor binding, compound eye morphogenesis, protein complex, protein binding, smoothened signaling pathway, neurogenesis, Wnt signaling pathway, glial cell migration, zinc ion binding, nucleus, neurotransmitter secretion, transcription cofactor activity, histone acetyltransferase activity, regulation of transcription, DNA-templated, histone H4-K12 acetylation, histone H4-K8 acetylation, histone H3-K27 acetylation, histone H3-K18 acetylation, histone acetyltransferase activity (H3-K18 specific), histone acetyltransferase activity (H3-K27 specific), cAMP response element binding protein binding, circadian regulation of gene expression, DNA replication checkpoint, R7 cell differentiation, transcription coactivator activity, regulation of mitosis ...
Early odor preference learning in rats is associated with increases of phosphorylated CREB (pCREB) in mitral cells of the olfactory bulb. In the present study, herpes simplex virus expressing CREB (HSV-CREB) and dominant-negative mutant CREB (HSV-mCREB) have been injected into the bulb to assess a causal role for CREB and pCREB in this model. Odor paired with stroking or with the β-adrenoceptor agonist isoproterenol produces odor approach 24 hr later. Isoproterenol-induced learning exhibits an inverted U curve dose-dependent learning relationship with both low and high doses failing to produce learning. pCREB increases have only been seen at the learning effective dose. In the present study, injection of an HSV vector expressing mutant CREB into the olfactory bulb prevented learning induced by stroking. Control HSV expressing LacZ was without effect. Expression of mutant CREB shifted the dose-learning curve for isoproterenol to the right such that a higher dose was required to induce learning. ...
Given the known roles of BET proteins as transcriptional co-activators, we next investigated whether BET inhibition led to changes in gene expression in T cells during TH17 polarization. Consistent with the failure of naive T cells to differentiate into TH17 cells in the presence of JQ1, we found the transcription of TH17 canonical genes, such as those encoding IL-17A, IL-17F, IL-21, IL-22, IL-23R, RORα, and RORγt, to be significantly reduced by quantitative RT-PCR (qPCR) analysis (unpublished data) after 48 h of polarization. Suppression of proinflammatory gene transcription was selective, as Tnf remained unchanged in the presence of JQ1. Furthermore, JQ1 had no effect on the expression of Il6r (unpublished data), complementing the data reported above (Fig. 2 D, top) for a role of BET in TH17 differentiation independent of early events in IL-6 signaling. These observations suggest that pharmacological blockade of BET bromodomains does not afford general gene suppression but rather a targeted ...
This is the place to find free crochet hat patterns. The page includes pictures of each hat so you can easily choose a project to work on ...

Specific role for p300/CREB-binding protein-associated factor activity in E2F1 stabilization in response to DNA damage<...Specific role for p300/CREB-binding protein-associated factor activity in E2F1 stabilization in response to DNA damage<...

Ianari A, Gallo R, Palma M, Alesse E, Gulino A. Specific role for p300/CREB-binding protein-associated factor activity in E2F1 ... Specific role for p300/CREB-binding protein-associated factor activity in E2F1 stabilization in response to DNA damage. In: ... Ianari, A, Gallo, R, Palma, M, Alesse, E & Gulino, A 2004, Specific role for p300/CREB-binding protein-associated factor ... Specific role for p300/CREB-binding protein-associated factor activity in E2F1 stabilization in response to DNA damage. / ...
more infohttps://moh-it.pure.elsevier.com/en/publications/specific-role-for-p300creb-binding-protein-associated-factor-acti

Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15<...Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15<...

Xie, F, Fan, Q, Li, BX & Xiao, X 2019, Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)- ... Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15. ... Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15 ... Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15 ...
more infohttps://ohsu.pure.elsevier.com/en/publications/discovery-of-a-synergistic-inhibitor-of-camp-response-element-bin

2rny - Proteopedia, life in 3D2rny - Proteopedia, life in 3D

Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)-mediated acetylation. J Biol Chem. 2001 Apr 6;276(14):10715-21. ... Chromosomal protein , Creb , Disease mutation , Dna-binding , Histone , Host-virus interaction , Metal-binding , Methylation , ... CREB-binding protein References *↑ Murata T, Kurokawa R, Krones A, Tatsumi K, Ishii M, Taki T, Masuno M, Ohashi H, Yanagisawa M ... Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its ...
more infohttp://proteopedia.org/wiki/index.php/2rny

CREBBP | SelfDecode | Genome AnalysisCREBBP | SelfDecode | Genome Analysis

CREB-binding protein, CREB binding protein (Rubinstein-Taybi syndrome), crebbp-a, crebbp-b, D623_10028045, E1A binding protein ... Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its ... Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair ( ... hypothetical protein, A306_06942, Anapl_13162, AS27_07110, CBP, CBP/p300, ...
more infohttps://www.selfdecode.com/gene/crebbp/

Genetics of Rubinstein-Taybi Syndrome Follow-up: Further Outpatient Care, Complications, PrognosisGenetics of Rubinstein-Taybi Syndrome Follow-up: Further Outpatient Care, Complications, Prognosis

Transgenic mice expressing a truncated form of CREB-binding protein (CBP) exhibit deficits in hippocampal synaptic plasticity ... Truncated CBP protein leads to classical Rubinstein-Taybi syndrome phenotypes in mice: implications for a dominant-negative ...
more infohttps://emedicine.medscape.com/article/948453-followup

Duplications of the critical Rubinstein-Taybi deletion region on chromosome 16p13.3 cause a novel recognisable syndrome |...Duplications of the critical Rubinstein-Taybi deletion region on chromosome 16p13.3 cause a novel recognisable syndrome |...

Curcumin, a novel p300/CREB-binding protein-specific inhibitor of acetyltransferase, represses the acetylation of histone/ ... nonhistone proteins and histone acetyltransferase-dependent chromatin transcription. J Biol Chem 2004;279:51163-71. ...
more infohttp://jmg.bmj.com/content/47/3/155

Gene | CREBBPGene | CREBBP

The CREB binding protein (CREBBP) acts as a transcriptional coactivator for many signal transduction pathways that play an ... Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature. 1994; 370(6486):223-6. PMID: 7913207 ... Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature. 1994; 370(6486):223-6. PMID: 7913207 ...
more infohttps://www.invitae.com/en/physician/genes/20705/

CREB-binding protein (Q92793) | InterPro | EMBL-EBICREB-binding protein (Q92793) | InterPro | EMBL-EBI

We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ...
more infohttp://www.ebi.ac.uk/interpro/protein/Q92793

CREB-Binding ProteinCREB-Binding Protein

2003). Intrathecal injection of cAMP response element binding protein (CREB) antisense. By Wise Young in forum Neuropathic Pain ... CREB-Binding Protein This sounds promising for the future....Wonder if exercise builds it up?. https://www.eurekalert.org/pub_ ... TAR DNA-binding protein 43 in neurodegenerative disease.. By wildwilly in forum Neurodegeneration Research ... 2001). RNA-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of ...
more infohttp://sci.rutgers.edu/forum/showthread.php?264425-CREB-Binding-Protein&s=979b54b2cf1ece4809b7620792861544&p=1877253&viewfull=1

CREB-binding protein/p300 are transcriptional coactivators of p65 | PNASCREB-binding protein/p300 are transcriptional coactivators of p65 | PNAS

CREB- binding protein;. CREB,. cAMP response element binding protein;. GST,. glutathione S-transferase;. CAT,. chloramphenicol ... CREB-binding protein (CBP) is a coactivator that interacts with the cAMP-response element binding protein (CREB), a process ... CREB-binding protein/p300 are transcriptional coactivators of p65. Mary E. Gerritsen, Amy J. Williams, Andrew S. Neish, Sarah ... CREB-binding protein/p300 are transcriptional coactivators of p65. Mary E. Gerritsen, Amy J. Williams, Andrew S. Neish, Sarah ...
more infohttps://www.pnas.org/content/94/7/2927?ijkey=e4cb9e3eab51f003381149d6703ec6cbabd33ad7&keytype2=tf_ipsecsha

PREDICTED: CREB-binding protein-like [Danio rerio] - Protein - NCBIPREDICTED: CREB-binding protein-like [Danio rerio] - Protein - NCBI

PREDICTED: CREB-binding protein-like [Danio rerio] PREDICTED: CREB-binding protein-like [Danio rerio]. gi,528473282,ref,XP_ ... PREDICTED: CREB-binding protein-like [Danio rerio]. NCBI Reference Sequence: XP_005163780.1 ... The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in ... The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in ...
more infohttps://www.ncbi.nlm.nih.gov/protein/XP_005163780.1?report=GenPept

CREB-binding protein - WikipediaCREB-binding protein - Wikipedia

CREB-binding protein, also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene. The CREB protein ... "Histone binding protein RbAp48 interacts with a complex of CREB binding protein and phosphorylated CREB". Molecular and ... CREB-binding protein has been shown to interact with: TF2, AR, AIRE, BRCA1, C-jun, CSK, Ccaat-enhancer-binding proteins, CDX2, ... First isolated as a nuclear protein that binds to cAMP-response element-binding protein (CREB), this gene is now known to play ...
more infohttps://en.wikipedia.org/wiki/CREB-binding_protein

CREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila | GeneticsCREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila | Genetics

Drosophila CREB binding protein (CBP), which is encoded by the nejire (nej) locus, belongs to the CBP/p300 family of proteins ( ... and Dach proteins mediated through CREB binding protein. Mol. Cell. Biol. 22: 6759-6766. ... CREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila. Justin P. Kumar, Tazeen Jamal, Alex ... CREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila. Justin P. Kumar, Tazeen Jamal, Alex ...
more infohttp://www.genetics.org/content/168/2/877

Localization of nascent RNA and CREB binding protein with the PML-containing nuclear body | PNASLocalization of nascent RNA and CREB binding protein with the PML-containing nuclear body | PNAS

The Kaposis Sarcoma-Associated Herpesvirus K8 Protein Interacts with CREB-Binding Protein (CBP) and Represses CBP-Mediated ... The Epstein-Barr Virus BZLF1 Protein Interacts Physically and Functionally with the Histone Acetylase CREB-Binding Protein ... CREB binding protein;. FITC,. fluorescein isothiocyanate;. aa,. amino acid(s);. pol I and II,. polymerase I and II;. GFP,. ... Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in ...
more infohttps://www.pnas.org/content/95/9/4991?ijkey=035e8e49b77e33c126b391fadc4f8c8ef6aed4f0&keytype2=tf_ipsecsha

Down-regulation of CREB-binding protein expression inhibits thrombin-induced proliferation of endothelial cells: possible...Down-regulation of CREB-binding protein expression inhibits thrombin-induced proliferation of endothelial cells: possible...

If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
more infohttp://onlinelibrary.wiley.com/doi/10.1042/CBI20090304/pdf

CREB-Binding Protein Acetylates Hematopoietic Transcription Factor GATA-1 at Functionally Important Sites | Molecular and...CREB-Binding Protein Acetylates Hematopoietic Transcription Factor GATA-1 at Functionally Important Sites | Molecular and...

We have previously shown that the transcriptional cofactor CREB-binding protein (CBP) binds to the zinc finger domain of GATA-1 ... CREB-Binding Protein Acetylates Hematopoietic Transcription Factor GATA-1 at Functionally Important Sites. Hsiao-Ling Hung, ... 1996) CREB-binding protein activates transcription through multiple domains. J. Biol. Chem. 271:28138-28145. ... 1998) CREB-binding protein (CBP) cooperates with transcription factor GATA-1 and is required for erythroid differentiation. ...
more infohttps://mcb.asm.org/content/19/5/3496?ijkey=9517af90c6376297f6f96a7c5af034e60ae85cf1&keytype2=tf_ipsecsha

Immunohistochemical Expression of p53 Protein and CREB-binding Protein in Polyps and Adenocarcinomas of ColonImmunohistochemical Expression of p53 Protein and CREB-binding Protein in Polyps and Adenocarcinomas of Colon

The CREB-binding protein(CBP) is a transcriptional co-activators of various sequence-specific DNA-binding transcription factors ... Immunohistochemical Expression of p53 Protein and CREB-binding Protein in Polyps and Adenocarcinomas of Colon. ... RESULTS: 1. p53 protein expression was observed in 15% (9/60) of hyperplastic polyps, in 68.9% (124/180) of adenomatous polyps ... This article examined the expression levels of the p53 protein and CBP as well as their diagnostic value in a biopsy sample. ...
more infohttps://koreamed.org/article/0037JKSS/2005.69.1.48

RCSB PDB - 1KDX: KIX DOMAIN OF MOUSE CBP (CREB BINDING PROTEIN) IN COMPLEX WITH PHOSPHORYLATED KINASE INDUCIBLE DOMAIN (PKID)...RCSB PDB - 1KDX: KIX DOMAIN OF MOUSE CBP (CREB BINDING PROTEIN) IN COMPLEX WITH PHOSPHORYLATED KINASE INDUCIBLE DOMAIN (PKID)...

OF RAT CREB (CYCLIC AMP RESPONSE ELEMENT BINDING PROTEIN), NMR 17 STRUCTURES ... CREB BINDING PROTEIN) IN COMPLEX WITH PHOSPHORYLATED KINASE INDUCIBLE DOMAIN (PKID) ... KIX DOMAIN OF MOUSE CBP (CREB BINDING PROTEIN) IN COMPLEX WITH PHOSPHORYLATED KINASE INDUCIBLE DOMAIN (PKID) OF RAT CREB ( ... Solution structure of the KIX domain of CBP bound to the transactivation domain of CREB: a model for activator:coactivator ...
more infohttps://www.rcsb.org/structure/1KDX

Interaction of Human Immunodeficiency Virus Type 1 Tat with the Transcriptional Coactivators p300 and CREB Binding Protein |...Interaction of Human Immunodeficiency Virus Type 1 Tat with the Transcriptional Coactivators p300 and CREB Binding Protein |...

GST-Tat was also used in binding studies with other proteins, including RelA and Stat2. Neither of these proteins bound to GST- ... 1993) Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature 365:855-859. ... Interaction of Human Immunodeficiency Virus Type 1 Tat with the Transcriptional Coactivators p300 and CREB Binding Protein. ... Among the factors associated with the basal transcription complex, p300 and the related CREB binding protein (CBP) (2) have ...
more infohttps://jvi.asm.org/content/72/10/8252?ijkey=fd5790a5043a0b944e7d95c56b7fa669292b5eb1&keytype2=tf_ipsecsha

A Lipopolysaccharide-Specific Enhancer Complex Involving Ets, Elk-1, Sp1, and CREB Binding Protein and p300 Is Recruited to the...A Lipopolysaccharide-Specific Enhancer Complex Involving Ets, Elk-1, Sp1, and CREB Binding Protein and p300 Is Recruited to the...

1998) cAMP-response-element-binding-protein-binding protein (CBP) and p300 are transcriptional co-activators of early growth ... we show that LPS-mediated TNF-α transcription is dependent upon CREB binding protein (CBP) and p300 coactivator proteins and, ... assembly of the LPS-stimulated TNF-α enhancer complex is dependent upon the coactivator proteins CREB binding protein and p300 ... 1993) Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature 365:855-859. ...
more infohttps://mcb.asm.org/content/20/16/6084?ijkey=0823e1da91978c3d2d3afd4c84068fd26ff2d2a9&keytype2=tf_ipsecsha

HIV-1 tat transactivator recruits p300 and CREB-binding protein histone acetyltransferases to the viral promoter. - Semantic...HIV-1 tat transactivator recruits p300 and CREB-binding protein histone acetyltransferases to the viral promoter. - Semantic...

... the transcriptional coactivator p300 and the closely related CREB-binding protein (CBP), having histone acetyltransferase (HAT ... The HIV-1 Tat protein binds to a stem-loop structure at the 5 end of viral mRNA and relieves this inhibition by inducing a ... Tat associates with HAT activity in human nuclear extracts and binds to p300 and CBP both in vitro and in vivo. Integrity of ... the integrated viral promoter is present in a chromatin-bound conformation and is transcriptionally silent in the absence of ...
more infohttps://www.semanticscholar.org/paper/HIV-1-tat-transactivator-recruits-p300-and-protein-Marzio-Tyagi/9deac158402b614a737b4e599d57b17d46406842

The transcriptional cofactor MIER1-beta negatively regulates histone acetyltransferase activity of the CREB-binding protein |...The transcriptional cofactor MIER1-beta negatively regulates histone acetyltransferase activity of the CREB-binding protein |...

Creb-binding protein (CBP). Using GST pull-down assays, we demonstrate that MIER1 interacts with CBP and that this interaction ... Two major protein isoforms have been identified, MIER1α and β, which differ in their C-terminal sequence. Previously, we ... Histone 4 peptide binding assays demonstrate that this inhibition of HAT activity is not the result of interference with ... Functional analysis, using HEK293 cells, shows that the CBP bound to MIER1 in vivo has no detectable HAT activity. ...
more infohttps://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-1-68

Prothymosin α interacts with the CREB‐binding protein and potentiates transcription | EMBO ReportsProthymosin α interacts with the CREBbinding protein and potentiates transcription | EMBO Reports

Gerritsen, M.E., Williams, A.J., Neish, A.S., Moore, S., Shi, Y. and Collins, T. (1997) CREBbinding protein/p300 are ... von Mikecz, A., Zhang, S., Montminy, M., Tan, E.M. and Hemmerich, P. (2000) CREBbinding protein (CBP)/p300 and RNA polymerase ... Prothymosin α interacts with the CREBbinding protein and potentiates transcription. Zoe Karetsou, Adroniki Kretsovali, Carol ... We report here that ProTα physically interacts with the CREBbinding protein (CBP), which is a versatile transcription co‐ ...
more infohttp://embor.embopress.org/content/3/4/361

Multiple Functions of CREB-Binding Protein During Postembryonic Develo by Amit Roy, Smitha George et al."Multiple Functions of CREB-Binding Protein During Postembryonic Develo" by Amit Roy, Smitha George et al.

CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including ... Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins ... CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including ... Multiple Functions of CREB-Binding Protein During Postembryonic Development: Identification of Target Genes ...
more infohttps://uknowledge.uky.edu/entomology_facpub/128/

Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2. - Radcliffe...Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2. - Radcliffe...

The transcriptional co-activators and histone acetyltransferases p300/CREB-binding protein (CBP) interact with CITED2, a ... DNA-Binding Proteins, Dimerization, E1A-Associated p300 Protein, Mice, Nuclear Proteins, Precipitin Tests, Repressor Proteins, ... Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2. ... Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2. ...
more infohttps://www.rdm.ox.ac.uk/publications/110443
  • Finally, we report that the formation of E2F1-p300/CREB-binding protein-associated factor (P/CAF) complexes is preferentially induced in doxorubicin-treated cells, and that P/CAF acetyltransferase (HAT), but not p300 HAT activity, is required for a significant E2F1 stabilization and accumulation. (elsevier.com)
  • Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB. (elsevier.com)
  • Similar polyacidic tracks are found in several nuclear proteins whose functions are linked to chromatin decondensation, nucleosome assembly/disassembly and establishment of chromatin structures competent for transcription ( Earnshaw, 1987 ). (embopress.org)
  • This protein shares regions of very high-sequence similarity with protein EP300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. (wikipedia.org)
  • A small molecule inhibitor (I-CBP112) binding to the bromodomain domain of CBP/p300 has been developed for leukaemia therapy. (wikipedia.org)
  • In the cell, NF-κB exists as homo- or heterodimers with distinct DNA binding specificities (for review, see refs. (pnas.org)
  • CREB binding protein [Homo sapi. (imp.ac.at)
  • Novel genetic variants in HDAC2 and PPARGC1A of the CREB-binding protein pathway predict survival of non-small-cell lung cancer. (cdc.gov)
  • monocyte chemotactic protein-1 enzymes (inducible nitric oxide synthase and cyclooxygenase II), and the endothelial-leukocyte adhesion molecules [intercellular adhesion molecule 1, vascular cell adhesion molecule 1 (VCAM-1), and E-selectin]. (pnas.org)
  • D ) Identification of the CBP‐binding site within the ProTα molecule. (embopress.org)
  • The adenovirus E1A 12S protein, which complexes with CBP and p300, inhibited p65-dependent gene expression. (pnas.org)
  • Finally, we report that the formation of E2F1-p300/CREB-binding protein-associated factor (P/CAF) complexes is preferentially induced in doxorubicin-treated cells, and that P/CAF acetyltransferase (HAT), but not p300 HAT activity, is required for a significant E2F1 stabilization and accumulation. (elsevier.com)
  • CITED4 and p300/CREB-binding protein are present in endogenous naturally occurring complexes, indicating that they interact physiologically. (ox.ac.uk)
  • The carboxy-terminal region of p300, which binds to E1A, was shown to bind specifically to the highly conserved basic domain of Tat, which also mediates binding to the Tat-responsive region RNA stem-loop structure. (asm.org)
  • The HIV-1 Tat protein binds to a stem-loop structure at the 5' end of viral mRNA and relieves this inhibition by inducing a remodeling of the nucleosome arrangement downstream of the transcription-initiation site. (semanticscholar.org)
  • Strikingly, Ets and Elk-1 bind to two TNF-α nuclear factor of activated T cells (NFAT)-binding sites, which are required for calcineurin and NFAT-dependent TNF-α gene expression in lymphocytes. (asm.org)
  • Remarkably, a set of TNF-α promoter elements, which bind NFAT upon induction of the gene by calcineurin-dependent stimuli, also bind the ERK-targeted Ets and Elk proteins and are required in LPS-stimulated TNF-α gene expression. (asm.org)
  • Restoration of CREB function is linked to completion and stabilization of adaptive cardiac hypertrophy in response to exercise. (semanticscholar.org)
  • Direct physical interactions of CBP/p300 with p65 were demonstrated by glutathione S -transferase fusion protein binding, and coimmunoprecipitation/Western blot studies. (pnas.org)
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
  • 2001). RNA-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. (rutgers.edu)
  • The expression patterns of specific DNA-binding factors that control eye development add an additional layer of complexity ( K umar and M oses 1997 ). (genetics.org)
  • For PML-green fluorescent protein (GFP) expression, cells were microinjected with a CMX PML-GFP DNA expression construct at 50-200 ng/μl and incubated overnight to allow for expression. (pnas.org)
  • This article examined the expression levels of the p53 protein and CBP as well as their diagnostic value in a biopsy sample. (koreamed.org)
  • The transcription factors ATF-2, c-jun, Egr-1, and Sp1 are also inducibly recruited to the TNF-α promoter in vivo, and the binding sites for each of these activators are required for LPS-stimulated TNF-α gene expression. (asm.org)
  • We propose that CBP may participate in cAMP-regulated gene expression by interacting with the activated phosphorylated form of CREB. (nih.gov)
  • This results in the production of the PML-retinoic acid receptor fusion protein, which is the presumed etiologic agent of acute promyelocytic leukemia ( 3 - 12 ). (pnas.org)
  • In LPS-stimulated macrophages, the ERK substrates Ets and Elk-1 bind to the TNF-α promoter in vivo. (asm.org)