A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
The organ of sight constituting a pair of globular organs made up of a three-layered roughly spherical structure specialized for receiving and responding to light.
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
A species of fruit fly much used in genetics because of the large size of its chromosomes.
Specialized cells in the invertebrates that detect and transduce light. They are predominantly rhabdomeric with an array of photosensitive microvilli. Illumination depolarizes invertebrate photoreceptors by stimulating Na+ influx across the plasma membrane.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
A member of the p300-CBP transcription factors that was originally identified as a binding partner for ADENOVIRUS E1A PROTEINS.
Enzymes that catalyze acyl group transfer from ACETYL-CoA to HISTONES forming CoA and acetyl-histones.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
Profound physical changes during maturation of living organisms from the immature forms to the adult forms, such as from TADPOLES to frogs; caterpillars to BUTTERFLIES.
Enzymes catalyzing the transfer of an acetyl group, usually from acetyl coenzyme A, to another compound. EC 2.3.1.
Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.
Formation of an acetyl derivative. (Stedman, 25th ed)

The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus. (1/1067)

Latent Epstein-Barr virus (EBV) is maintained as a nucleosome-covered episome that can be transcriptionally activated by overexpression of the viral immediate-early protein, Zta. We show here that reactivation of latent EBV by Zta can be significantly enhanced by coexpression of the cellular coactivators CREB binding protein (CBP) and p300. A stable complex containing both Zta and CBP could be isolated from lytically stimulated, but not latently infected RAJI nuclear extracts. Zta-mediated viral reactivation and transcriptional activation were both significantly inhibited by coexpression of the E1A 12S protein but not by an N-terminal deletion mutation of E1A (E1ADelta2-36), which fails to bind CBP. Zta bound directly to two related cysteine- and histidine-rich domains of CBP, referred to as C/H1 and C/H3. These domains both interacted specifically with the transcriptional activation domain of Zta in an electrophoretic mobility shift assay. Interestingly, we found that the C/H3 domain was a potent dominant negative inhibitor of Zta transcriptional activation function. In contrast, an amino-terminal fragment containing the C/H1 domain was sufficient for coactivation of Zta transcription and viral reactivation function. Thus, CBP can stimulate the transcription of latent EBV in a histone acetyltransferase-independent manner mediated by the CBP amino-terminal C/H1-containing domain. We propose that CBP may regulate aspects of EBV latency and reactivation by integrating cellular signals mediated by competitive interactions between C/H1, C/H3, and the Zta activation domain.  (+info)

The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness. (2/1067)

Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha-stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha-inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness.  (+info)

Differences in the interactions of oncogenic adenovirus 12 early region 1A and nononcogenic adenovirus 2 early region 1A with the cellular coactivators p300 and CBP. (3/1067)

Association with the cellular coactivators p300 and CBP is required for the growth-regulatory function of adenoviral (Ad) early region 1A (E1A) proteins. E1A regions necessary for these interactions overlap with domains involved in the induction of tumours in immunocompetent rodents through highly oncogenic Ad12. Differences in the association of cellular factors with the respective E1A domains of Ad12 and nononcogenic Ad2 might therefore be involved in serotype-specific oncogenicity. We analyzed the interaction of the Ad12 E1A 235R protein with p300 and CBP. Here we demonstrate that in the case of Ad12, but not Ad2/5, amino acids (aa) 1-29 of E1A proteins are sufficient to bind the p300-C/H3 domain in vivo and wild-type p300 in vitro. The conserved arginine-2, which is essential for the interaction between Ad2 E1A and p300, was dispensable for the Ad12 E1A 235R-p300 interaction in vitro. In addition to the p300-C/H3 region, we identified a second domain within p300 (aa 1999-2200) binding to the 235R protein. Contrary to p300, the amino-terminus and CR1 are necessary to associate with CBP. The aa 1-29 of the 235R protein but not CR1 are essential for the repression of colTRE-driven gene expression. This repression function is strictly dependent on p300 but not on CBP.  (+info)

Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP. (4/1067)

Yeast two-hybrid system was employed to isolate novel proteins that physically interact with PU.1, a member of Ets family transcription factors. Sequence analyses of several isolated clones positive for beta-galactosidase activity revealed that one of these clones was confirmed to encode a transcriptional coactivator, CREB binding protein (CBP). GST binding assay showed that the interacting sites were located at the transcriptional activation domain of PU.1 through 74-122 and the region spanning residues 1283-1915 of CBP. CBP potentiated PU.1-mediated transcription of the reporter gene driven by the multimerized PU.1-binding sites, suggesting that CBP functions as a coactivator for PU.1. Considering that CBP is a limited cellular component to function as a coactivator for several transcription factors, CBP may mediate synergistic and antagonistic interactions between PU.1 and other transcription factors during the process of hematopoietic cell differentiation.  (+info)

c-Jun functions as a calcium-regulated transcriptional activator in the absence of JNK/SAPK1 activation. (5/1067)

Calcium is the principal second messenger in the control of gene expression by electrical activity in neurons. Recruitment of the coactivator CREB-binding protein, CBP, by the prototypical calcium-responsive transcription factor, CREB and stimulation of CBP activity by nuclear calcium signals is one mechanism through which calcium influx into excitable cells activates gene expression. Here we show that another CBP-interacting transcription factor, c-Jun, can mediate transcriptional activation upon activation of L-type voltage-gated calcium channels. Calcium-activated transcription mediated by c-Jun functions in the absence of stimulation of the c-Jun N-terminal protein kinase (JNK/SAPK1) signalling pathway and does not require c-Jun amino acid residues Ser63 and Ser73, the two major phosphorylation sites that regulate c-Jun activity in response to stress signals. Similar to CREB-mediated transcription, activation of c-Jun-mediated transcription by calcium signals requires calcium/ calmodulin-dependent protein kinases and is dependent on CBP function. These results identify c-Jun as a calcium-regulated transcriptional activator and suggest that control of coactivator function (i.e. recruitment of CBP and stimulation of CBP activity) is a general mechanism for gene regulation by calcium signals.  (+info)

Modulation of CREB binding protein function by the promyelocytic (PML) oncoprotein suggests a role for nuclear bodies in hormone signaling. (6/1067)

Disaggregation of the spherical nuclear bodies termed promyelocytic (PML) oncogenic domains (PODs) is a characteristic of acute promyelocytic leukemia. Here, we demonstrate that the cAMP enhancer binding protein (CREB)-binding protein (CBP) associates with PML in vitro and is recruited to the PODs in vivo. Through its association with CBP, wild-type PML dramatically stimulates nuclear receptor transcriptional activity. These results demonstrate that a fraction of CBP is compartmentalized to the POD through its association with PML and thus suggest that PML and other POD-associated proteins may play an unexpectedly broad role in aspects of transcriptional regulation and human disease.  (+info)

CREB binding protein coordinates the function of multiple transcription factors including nuclear factor I to regulate phosphoenolpyruvate carboxykinase (GTP) gene transcription. (7/1067)

Nuclear factor I (NFI) binds to a region of the phosphoenolpyruvate carboxykinase (GTP) (PEPCK) gene promoter adjacent to the cAMP regulatory element (CRE) and inhibits the induction of transcription from the gene promoter caused by the catalytic subunit of protein kinase A. In vivo footprinting studies demonstrated that both the CRE and the NFI-binding site are occupied by transcription factors, regardless of the presence of factors that stimulate (dibutyryl cAMP or dexamethasone) or inhibit (insulin) transcription from the PEPCK gene promoter. The NFI effects on transcription from the PEPCK gene promoter were observed even in the absence of the NFI binding site, suggesting the possibility of other weaker binding sites on the promoter or an interaction of NFI with a transcriptional co-activator. A mammalian two-hybrid system was used to demonstrate direct interaction between the transactivation domain of NFI-C and the CREB binding domain of the CREB-binding protein (CBP). Overexpression of a gene fragment encoding the CREB binding domain of CBP stimulates transcription from the PEPCK gene promoter. The inhibitory effect of NFI on transcription of the PEPCK gene induced by the catalytic subunit of protein kinase A appears to be the result of an interaction between NFI and the CREB-binding protein in which NFI competes with CREB for binding to the CREB-binding site on CBP. In contrast, glucocorticoids and thyroid hormone use the steroid hormone receptor binding domain of CBP to stimulate transcription from the PEPCK gene promoter. NFI-A combines with dexamethasone or thyroid hormone in an additive manner to stimulate PEPCK gene transcription. We conclude that CBP coordinates the action of the multiple factors known to control transcription of the PEPCK gene.  (+info)

CREB-binding protein is a transcriptional coactivator for hepatocyte nuclear factor-4 and enhances apolipoprotein gene expression. (8/1067)

Hepatocyte nuclear factor-4 (HNF-4) is a liver-enriched transcription factor that is crucial in the regulation of a large number of genes involved in glucose, cholesterol, and fatty acid metabolism and in determining the hepatic phenotype. We have previously shown that HNF-4 contains transcription activation functions at the N terminus (AF-1) and the C terminus (AF-2) which work synergistically to confer full HNF-4 activity. Here, we show that HNF-4 recruits the CREB-binding protein (CBP) coactivator on promoters of genes that contain functional HNF-4 sites. HNF-4 interacts with the N-terminal region of CBP (amino acids 1-771) and the C-terminal region of CBP (amino acids 1812-2441). The two activating functions of HNF-4, AF-1 and AF-2, interact with the N terminus and the N and C terminus of CBP, respectively. In addition, we show that in contrast to the other nuclear hormone receptors the interaction between HNF-4 and CBP is ligand-independent. Recruitment of CBP by HNF-4 results in an enhancement of the transcriptional activity of the latter. CBP does not activate gene expression in the absence of HNF-4, and dominant negative forms of HNF-4 prevent transcriptional activation by CBP, suggesting that the mere recruitment of CBP by HNF-4 is not sufficient for enhancement of gene expression. These findings demonstrate that CBP acts as a transcriptional coactivator for HNF-4 and provide new insights into the regulatory function of HNF-4.  (+info)

Histone lysine acetylation is central to epigenetic control of gene transcription. Bromodomains of chromosomal proteins function as acetyl-lysine (Kac) binding domains. However, how bromodomains recognize site-specific histones remains unanswered. Here, we report three three-dimensional solution structures of the bromodomains of the human transcriptional coactivators CREB-binding protein (CBP) and p300/CBP-associated factor (PCAF) bound to peptides derived from histone acetylation sites at lysines 36 and 9 in H3, and lysine 20 in H4. From structural and biochemical binding analyses, we determine consensus histone recognition by the bromodomains of PCAF and CBP, which represent two different subgroups of the bromodomain family. Through bromodomain residues in the ZA and BC loops, PCAF prefers acetylation sites with a hydrophobic residue at (Kac+2) position and a positively charged or aromatic residue at (Kac+3), whereas CBP favors bulky hydrophobic residues at (Kac+1) and (Kac+2), a positively ...
Members of the CREB-binding protein/p300-interacting transactivator with ED-rich tail (CITED) family bind CREB-binding protein and p300 with high affinity and regulate gene transcription. Gene knockout studies indicate that CITED2 is required for neural crest and neural tube development and that it functions as a co-activator for transcription factor AP-2 (TFAP2). Here we describe human CITED4, a new member of this family, which is encoded by a single exon mapping to chromosome 1p34--1p35. CITED4 and p300/CREB-binding protein are present in endogenous naturally occurring complexes, indicating that they interact physiologically. The interaction occurs between the cysteine-histidine-rich domain 1 of p300 and the carboxyl terminus of CITED4. In keeping with this, CITED4 functions as a transactivator when artificially targeted to a promoter element. CITED4 physically interacts with all TFAP2 isoforms in vitro and strongly co-activates all TFAP2 isoforms in Hep3B cells. Co-activation of TFAP2 requires amino
Mier1 encodes a novel transcriptional regulator and was originally isolated as a fibroblast growth factor early response gene. Two major protein isoforms have been identified, MIER1α and β, which differ in their C-terminal sequence. Previously, we demonstrated that both isoforms recruit histone deacetylase 1 (HDAC1) to repress transcription. To further explore the role of MIER1 in chromatin remodeling, we investigated the functional interaction of MIER1 with the histone acetyltransferase (HAT), Creb-binding protein (CBP). Using GST pull-down assays, we demonstrate that MIER1 interacts with CBP and that this interaction involves the N-terminal half (amino acids 1-283) of MIER1, which includes the acidic activation and ELM2 domains and the C-terminal half (amino acids 1094-2441) of CBP, which includes the bromo-, HAT, C/H3 and glutamine-rich domains. Functional analysis, using HEK293 cells, shows that the CBP bound to MIER1 in vivo has no detectable HAT activity. Histone 4 peptide binding assays
Avots, A., Buttmann, M., Chupvilo, S., Escher, C., Smola, U., Bannister, A.J., Rapp, U.R., Kouzarides, T., Serfling, E. CBP/p300 integrates Raf/Rac-signalling pathways in the transcriptional induction of NF-Atc during T cell activation Immunity 10: 515-524 (1999) Chirmule, N., Avots, A., LakshmiTamma, S.M., Pahwa, S., Serfling, E. CD4-mediated signals induce T cell dysfunction in vivo J. Immunol. 163: 644-649 (1999) Bannert, N., Avots, A., Baier, M., Serfling, E., Kurth, R. GA-binding protein factors, in concert with the coactivator CREB binding protein/p300, control the induction of the interleukin 16 promoter in T lymphocytes Proc. Natl. Acad. Sci. USA 96: 1541-1546 (1999) Chupvilo, S., Zimmer, M., Kerstan, A., Glöckner, J., Avots, A., Escher, C., Fischer, C., Inashkina, I., Jankevics, E., Berberich-Siebelt, F., Schmitt, E., Serfling, E. Alternative polyadenylation events contribute to the induction of NF-ATc in effector T cells Immunity 10: 261-269 (1999) Chupvilo, S., Avots, A., ...
Following the publication of this article [1], the authors found that the primers listed for CREB-binding protein were not correct. This mistake occurred during assembly of the primer table and the authors apologize for this error. This correction does not change the data included in the paper, their interpretation nor the conclusions drawn.
In the present study, we sought to examine the molecular basis for the differential regulation of several members of the IFN-α/β gene family (IFNA and IFNB) by IRF-3 and IRF-7. The IFNB, IFNA1, IFNA2, and RANTES promoters were activated by coexpression of either IRF-3 or IRF-7, whereas the IFNA4, IFNA7, and IFNA14 promoters were exclusively activated by IRF-7 and not by IRF-3. Analysis of protein-DNA interactions revealed that recombinant IRF-3 and IRF-7 selectively bound to different regions of the IFNB promoter; IRF-3 bound preferentially to the PRDIII domain of the IFNB promoter, while IRF-7 interacted exclusively with the PRDI domain. PCR-mediated DNA binding site selection results demonstrated that IRF-3 recognized the IRF consensus element 5′-GAAANNGAAANN-3′. Replacement of a single nucleotide within the GAAA core half-site was sufficient to preclude IRF-3 DNA binding. IRF-7 bound to a related sequence motif but with greater flexibility than IRF-3; a single nucleotide replacement did ...
The major finding in this study is that ethanol induces an increase in gene expression via CREB and PKA. This increase in gene expression requires both PKA and CREB phosphorylation. Although we had previously shown that exposure to ethanol resulted in phosphorylation of CREB in NG108-15 cells, there is accumulating evidence that CREB phosphorylation is not sufficient to regulate gene expression under the control of CREs; activation of the coactivator CREB-binding protein (CBP) and other downstream elements is also required for increases in CRE-mediated gene expression (Montminy, 1997; Cardinaux et al., 2000).Thibault et al. (2000) have reported increases in genes the expression of which is known to be cAMP-dependent. However, ethanol activates many different signal transduction pathways in addition to PKA (Diamond and Gordon, 1997), and most genes have regulatory elements activated or inhibited by all of these pathways. Therefore, the experiments presented here are the first demonstration that ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
We also aim to understand the structure-function relationship of very large (oversized) proteins, practically neglected from a structural point of view thus far. Structural biology has traditionally addressed the structure of small folded proteins, whereas the field of structural disorder has focused on either fully disordered proteins/regions or short disordered elements that undergo induced folding in the presence of their partner. Here we would like to probe into the structure of the very large transcriptional co-activator CREB-binding protein (CBP), by addressing its structure by means traditionally applied in the case of protein complexes. CBP has about seven domains and disordered linker regions connecting them, the topology of which will be outlined by a combination of high-resolution (NMR, X-ray) and low-resolution (MS, EM, AFM) techniques ...
Cancer, Epidermal Growth Factor, Gene, Growth, Survival, Cell Line, Tumors, Disease, Kinase, Mutation, Patient, Phenotype, Cell, Insulin, Mouse, Acetylation, Binding Protein, Creb-binding Protein, Neuroblastoma, Nuclear Export
Function: Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase ...
It does not matter how the diet is restricted. It can be in terms of carbohydrates,fats or proetins. What matters is the diet should be of low calorie. This brings about a change in the CBP proteins that control the genes related to cellular functions ...
The inappropriate sustained SNA increase in OSA patients likely contributes to hypertension, organ damage, and mortality; however, it is unclear how excessive SNA develops in these patients. Several factors, including obesity and increased carotid body chemoreceptor sensitivity due to intermittent hypoxia, have been considered. Obesity could mechanically obstruct the airway and increase SNA through leptin, insulin, angiotensin, and cytokine actions; however, many OSA patients are not obese (23). Carotid body hypersensitivity as a result of intermittent hypoxia has been confirmed in animal models of OSA. In fact, plasticity of the carotid body glomus cells with long-term sensory facilitation and sensitization have been reported (18, 24) and associated with ROS and NOX2-dependent accumulation of HIF1 and the transcriptional coactivator CREB-binding protein (25). Central neuroplasticity. A provocative possibility for OSA-associated SNA dysfunction is that excessive activation of CNS nuclei induces ...
Rubinstein-Taybi syndrome (RSTS) is an uncommon genetic disorder characterised by a typical facies, small stature, broad angulated thumbs and intellectual impairment. Dental changes are a minor, yet significant component of the condition. Craniofacial growth retardation in RSTS is frequently complicated by unerupted teeth, while dental caries is related to the inherent intellectual deficit. Dental problems necessitate interdisciplinary management in terms of oral surgery, conservative dentistry, periodontics and orthodontics. When affected individuals are unco-operative, certain dental procedures may warrant general anaesthesia. In these instances, dental and medical staff will combine their expertise to enhance the well-being of the patient. In addition, specific dental changes may alert the medical practitioner to the possible diagnosis of RSTS. In this article we document the oro-dental manifestations and review the oro-dental approach in the management of three patients with RSTS. Our experience in
Rubinstein, J. Broad thumb-hallux (Rubinstein-Taybi) Syndrome 1957-1988. Am J Med Gen Suppl . vol. 6. 1990. pp. 3-16. (An early review of 571 cases, this article provides a detailed description of the physical findings in this syndrome.). Wiley, S, Swayne, S, Rubinstein, J, Lanphear, N, Stevens, C. Rubinstein-Taybi syndrome medical guidelines. Am J Med Genet. vol. 119A. 2003. pp. 101-110. (This article includes specific surveillance and intervention recommendations compiled by a group of pediatric experts.). Cantani, A, Gagliesi, D. Rubinstein-Taybi syndrome. Review of 732 cases and analysis of typical traits. Eur Rev Med Pharmacol Sci. vol. 2. 1998. pp. 81-87. (This is an analysis of 732 cases and provides a summary of the physical findings of the syndrome and discusses epidemiology and genetics known at the time of publication.). Roelfsema, J, Peters, D. Rubinstein-Taybi syndrome: clinical and molecular overview. Expert Rev Mol Med. vol. 9. 2007. pp. 1-15. (This article details the ...
EP300-related Rubinstein-Taybi syndrome: Highlighted rare phenotypic findings and a genotype-phenotype meta-analysis of 74 patients.
Mutations in the coactivator CREB-binding protein (CBP) are a major cause of the human skeletal dysplasia Rubinstein-Taybi syndrome (RTS); however, the mechanism by which these mutations affect skeletal mineralization and patterning is unknown. Here, we report the identification of 3-phosphoinositide-dependent kinase 1 (PDK1) as a key regulator of CBP activity and demonstrate that its functions map to both osteoprogenitor cells and mature osteoblasts. In osteoblasts, PDK1 activated the CREB/CBP complex, which in turn controlled runt-related transcription factor 2 (RUNX2) activation and expression of bone morphogenetic protein 2 (BMP2). These pathways also operated in vivo, as evidenced by recapitulation of RTS spectrum phenotypes with osteoblast-specific Pdk1 deletion in mice (Pdk1osx mice) and by the genetic interactions observed in mice heterozygous for both osteoblast-specific Pdk1 deletion and either Runx2 or Creb deletion. Finally, treatment of Pdk1osx and Cbp+/- embryos with BMPs in utero ...
hypothetical protein, A306_06942, Anapl_13162, AS27_07110, CBP, CBP/p300, CREB-binding protein, CREB binding protein (Rubinstein-Taybi syndrome), crebbp-a, crebbp-b, D623_10028045, E1A binding protein p300, EP300, H920_13788, hmm291030, KAT3A, M91_18874, MDA_GLEAN10009599, N301_13283, N302_12939, N303_04372, N307_13277, N308_10632, N309_02966, N311_11763, N312_01973, N321_00697, N326_12400, N327_01513, N332_08465, N334_05471, N335_14336, N336_02992, N339_02947, p300, p300/CBP, PAL_GLEAN10011621, RSTS, RTS, UY3_13419, Y1Q_016907, Z169_09090, crebbp ...
TY - JOUR. T1 - Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15. AU - Xie, Fuchun. AU - Fan, Qiuhua. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. PY - 2019/1/1. Y1 - 2019/1/1. N2 - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, we designed an ester prodrug of 666-15 through a long-range O,N-acyl transfer reaction for improved aqueous solubility. Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB.. AB - CREB is a transcription factor implicated in the pathogenesis of multiple cancers. Targeting CREB is a promising strategy to develop potential cancer therapeutics. Previously, we identified 666-15 as a potent CREB inhibitor. Herein, ...
Hop on to get the meaning of p/ CIP acronym / slang / Abbreviation. The Medical & Science Acronym / Slang p/ CIP means... AcronymsAndSlang. The p/ CIP acronym/abbreviation definition. The p/ CIP meaning is p300/ CREB-binding protein (CBP)-interacting protein. The definition of p/ CIP by AcronymAndSlang.com
TY - JOUR. T1 - Nuclear factor-kappaB and cAMP response element binding protein mediate opposite transcriptional effects on the Flk-1/KDR gene promoter.. AU - Illi, B.. AU - Puri, P.. AU - Morgante, L.. AU - Capogrossi, M. C.. AU - Gaetano, C.. PY - 2000/6/23. Y1 - 2000/6/23. N2 - -The vascular endothelial growth factor receptor Flk-1/KDR is highly expressed during development and almost disappears in adult tissues. Despite its biological relevance, little is known about the molecular mechanisms controlling its expression. In the present work, it is shown that cAMP response element binding protein (CREB) and nuclear factor-kappaB (NF-kappaB)-related antigens bind specific sequences in the Flk-1/KDR promoter. Functional studies demonstrate that cAMP represses whereas tumor necrosis factor-alpha, an activator of NF-kappaB, stimulates promoter activity. Histone acetyltransferases (HATs) P/CAF and CBP/p300 together with p65/RelA, the catalytic subunit of NF-kappaB, increase Flk-1/KDR promoter ...
CREBBP is targeted by inactivating mutations in follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL). Here, we provide evidence from transgenic mouse models that Crebbp deletion results in deficits in B-cell development and can cooperate with Bcl2 overexpression to promote B-cell lymphoma. Through transcriptional and epigenetic profiling of these B cells, we found that Crebbp inactivation was associated with broad transcriptional alterations, but no changes in the patterns of histone acetylation at the proximal regulatory regions of these genes. However, B cells with Crebbp inactivation showed high expression of Myc and patterns of altered histone acetylation that were localized to intragenic regions, enriched for Myc DNA binding motifs, and showed Myc binding. Through the analysis of CREBBP mutations from a large cohort of primary human FL and DLBCL, we show a significant difference in the spectrum of CREBBP mutations in these 2 diseases, with higher frequencies of ...
The CREBBP gene is associated with autosomal dominant Rubinstein-Taybi syndrome 1 (RSTS1) (MedGen UID: 48517) and is commonly deleted in the recurrent 16p13.3 microdeletion syndrome (OMIM: 610543), a severe form of RSTS resulting from a contiguous gene deletion involving CREBBP as well as other neighboring genes.
In 1963, Rubinstein and Taybi first described a malformation syndrome characterized by distinctive facies, mental retardation, broad thumbs, and broad great toes as are seen in the images below. {file44122}{file44123}{file44124}Deletions in band 16p13 have been described in association with this disorder, and mutations in the cyclic adenosin...
CREBBP - CREBBP (Myc-DDK-tagged)-Human CREB binding protein (CREBBP), transcript variant 1 available for purchase from OriGene - Your Gene Company.
Sigma-Aldrich offers abstracts and full-text articles by [Q Li, H Peng, H Fan, X Zou, Q Liu, Y Zhang, H Xu, Y Chu, C Wang, K Ayyanathan, F J Rauscher, K Zhang, Z Hou].
The concentration of glucose in the bloodstream is regulated by glucose itself, along with the hormones insulin and glucagon. Glucagon stimulates gluconeogenesis in part by regulating phosphorylation of a transcriptional coactivator known as cyclic adenosine monophosphate response element-binding protein 2 (CRTC2). Dentin et al. (see the Perspective by Birnbaum) found that high concentrations of circulating glucose also regulate CRTC2, but do so through stimulation of the hexosamine biosynthetic pathway and consequent O-linked glycosylation of the same serine residue in CRTC2 that is modified by phosphorylation. Thus, CRTC2 integrates signals from hormones and nutrients and might be a target for efforts to treat abnormalities of glucose homeostasis that are associated with diabetes.. R. Dentin, S. Hedrick, J. Xie, J. Yates, III, M. Montminy, Hepatic glucose sensing via the CREB coactivator CRTC2. Science 319, 1402-1405 (2008). [Abstract] [Full Text]. M. J. Birnbaum, Sweet conundrum. Science 319, ...
Lysine propionylation and butyrylation are protein modifications that were recently identified in histones. The molecular components involved in the two protein modification pathways are unknown, hindering further functional studies. Here we report identification of the first three in vivo non-histone protein substrates of lysine propionylation in eukaryotic cells: p53, p300, and CREB-binding protein. We used mass spectrometry to map lysine propionylation sites within these three proteins. We also identified the first two in vivo eukaryotic lysine propionyltransferases, p300 and CREB-binding protein, and the first eukaryotic depropionylase, Sirt1. p300 was able to perform autopropionylation on lysine residues in cells. Our results suggest that lysine propionylation, like lysine acetylation, is a dynamic and regulatory post-translational modification. Based on these observations, it appears that some enzymes are common to the lysine propionylation and lysine acetylation regulatory pathways. Our ...
In type 2 diabetes, chronic hyperglycemia is detrimental to beta-cells, causing apoptosis and impaired insulin secretion. The transcription factor cAMP-responsive element-binding protein (CREB) is crucial for beta-cell survival and function. We inves
TY - JOUR. T1 - Design, synthesis and biological evaluation of regioisomers of 666-15 as inhibitors of CREB-mediated gene transcription. AU - Xie, Fuchun. AU - Li, Bingbing X.. AU - Xiao, Xiangshu. N1 - Funding Information: This work was made possible through financial supports provided by the United States National Institutes of Health ( R01GM087305 ) and OHSU Office of Technology Transfer and Business Development . PY - 2017/2/1. Y1 - 2017/2/1. N2 - cAMP-response element binding protein (CREB) is a nuclear transcription factor that has been implicated in the pathogenesis and maintenance of various types of human cancers. Identification of small molecule inhibitors of CREB-mediated gene transcription has been pursued as a novel strategy for developing cancer therapeutics. We recently discovered a potent and cell-permeable CREB inhibitor called 666-15. 666-15 is a bisnaphthamide and has been shown to possess efficacious anti-breast cancer activity without toxicity in vivo. In this study, we ...
TY - JOUR. T1 - Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB. AU - Asahara, H.. AU - Santoso, B.. AU - Guzman, E.. AU - Du, K.. AU - Cole, P. A.. AU - Davidson, I.. AU - Montminy, M.. PY - 2001/11/22. Y1 - 2001/11/22. N2 - The cyclic AMP (cAMP)-responsive factor CREB induces target gene expression via constitutive (Q2) and inducible (KID, for kinase-inducible domain) activation domains that function synergistically in response to cellular signals. KID stimulates transcription via a phospho (Ser133)-dependent interaction with the coactivator paralogs CREB binding protein and p300, whereas Q2 recruits the TFIID complex via a direct association with hTAFII130. Here we investigate the mechanism underlying cooperativity between the Q2 domain and KID in CREB by in vitro transcription assay with naked DNA and chromatin templates containing the cAMP-responsive somatostatin promoter. The Q2 domain was highly active on a naked DNA template, and Ser133 ...
Extensive evidence implicates CREB-dependent gene transcription in memory (Bourtchuladze et al., 1994; Yin et al., 1994; Guzowski and McGaugh, 1997; Bartsch et al., 1998; Kida et al., 2002; Pittenger et al., 2002; Frankland et al., 2004). Multiple signaling pathways phosphorylate CREB at Ser133 (Shaywitz and Greenberg, 1999; Mayr and Montminy, 2001; Lonze and Ginty, 2002), which stimulates the recruitment of coactivators CBP/p300 (Chrivia et al., 1993; Parker et al., 1996). However, this phosphorylation event is not always sufficient to activate transcription (Impey et al., 1996; Mayr and Montminy, 2001) suggesting that CREB-mediated transcription is regulated by additional mechanisms.. In 2003, two laboratories identified a new family of CREB-specific coactivators, now referred to as CRTCs (Iourgenko et al., 2003; Conkright et al., 2003b). CRTC is thought to enhance transcription by facilitating the interaction of CREB with the RNA polymerase II pre-initiation complex (Conkright et al., 2003b; ...
The AlphaLISA SureFire Ultra p-CREB (Ser133) assay kit (High Volume) is an immunoassay for quantitative detection of phospho-CREB in cellular lysates.
I remember the fateful day when I saw my first Kix Commercial with its slogan Kid Tested, Mother Approved. In my ten-year-old mind, Id finally found the loophole in the cruel system of parental cereal control. Somehow, my mom actually obeyed this mantra. Kix occasionally landed in our cabinets, a brightly colored beacon in a sea of white and brown boxes. Recently, I bought a box to see if they were still as great as I remembered.
Rubinstein-Taybi Syndrome is a rare condition affecting 1:125,000 children. It is associated with short broad radially deviated thumbs, secondary to a delta proximal phalanx of the thumb. We undertook a retrospective review of seven children (13 thumbs) with Rubinstein-Taybi syndrome whose thumbs were treated using a corrective osteotomy to the delta phalanx over a 13 year period. The types of osteotomy used in the series were reverse wedge osteotomy, opening wedge osteotomy and dome shaped osteotomy. The mean preoperative radial deviation of thumbs was 68 degrees (range 45-85 degrees ). At follow up five of the 13 thumbs demonstrated some residual radial deviation. All recurrences occurred in the dome shaped osteotomy group. Our data suggest that surgery is effective in correcting the deformity, but there is a risk of incomplete correction or recurrence. Despite the recurrence the mean postoperative deformity was significantly better than preoperatively and the majority of patients families
Looking for online definition of cAMP-responsive element-binding protein 3-like protein 4 in the Medical Dictionary? cAMP-responsive element-binding protein 3-like protein 4 explanation free. What is cAMP-responsive element-binding protein 3-like protein 4? Meaning of cAMP-responsive element-binding protein 3-like protein 4 medical term. What does cAMP-responsive element-binding protein 3-like protein 4 mean?
Looking for online definition of cAMP-responsive element-binding protein 3-like protein 1 in the Medical Dictionary? cAMP-responsive element-binding protein 3-like protein 1 explanation free. What is cAMP-responsive element-binding protein 3-like protein 1? Meaning of cAMP-responsive element-binding protein 3-like protein 1 medical term. What does cAMP-responsive element-binding protein 3-like protein 1 mean?
Most reported microdeletions of the CREB-binding protein (CBP) gene in the Rubinstein-Taybi syndrome (RTS) were detected by fluorescence in situ hybridization (FISH) with a single cosmid probe specific to the 3 region of the gene. In order to test the hypothesis that the rate of microdeletion-posit …
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
TY - JOUR. T1 - The transcriptional coactivator and acetyltransferase p300 in fibroblast biology and fibrosis. AU - Ghosh, Asish K.. AU - Varga, John. PY - 2007/12/1. Y1 - 2007/12/1. N2 - The transcriptional coactivator p300 is a ubiquitous nuclear phosphoprotein and transcriptional cofactor with intrinsic acetyltransferase activity. p300 controls the expression of numerous genes in cell-type and signal-specific manner, and plays a pivotal role in cellular proliferation, apoptosis, and embryogenesis. By catalyzing acetylation of histones and transcription factors, p300 plays a significant role in epigenetic regulation. Recent evidence suggests that abnormal p300 function is associated with deregulated target gene expression, and is implicated in inflammation, cancer, cardiac hypertrophy, and genetic disorders such as the Rubinstein-Taybi syndrome. The activity of p300 is regulated at multiple levels, including developmental stage-specific expression, post-translational modifications, subcellular ...
4-PPBP is a molecule which binds to sigma receptors.[1] 4-PPBP decreases neuronal nitric oxide synthase (nNOS) activity and ischemia-evoked nitric oxide (NO) production. 4-PPBP provides neuroprotection; this involves the prevention of ischemia-induced intracellular Ca2+dysregulation.[2]4-PPBP protects neurons using a mechanism that activates the transcription factor cyclic adenosine monophosphate response element-binding protein (CREB). Neuroprotection that is associated with 4-PPBP increases Bcl-2 expression; Bcl-2 expression is regulated by CREB. [3] ...
RefSeq Summary (NM_001675): This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011 ...
Incubation of 3T3-L1 preadipocytes with isobutylmethylxanthine (IBMX), dexamethasone, and insulin, alone or in combination, demonstrated that IBMX, which increased cAMP-response element-binding protein (CREB) phosphorylation, was the predominant regulator of Pde3b expression. Real time PCR and immunoblotting indicated that in 3T3-L1 preadipocytes, IBMX-stimulated induction of Pde3b mRNA and protein was markedly inhibited by dominant-negative CREB proteins. By transfecting preadipocytes, differentiating preadipocytes, and HEK293A cells with luciferase reporter vectors containing different fragments of the 5- flanking region of the Pde3b gene, we identified a distal promoter that contained canonical cis-acting cAMP-response elements (CRE) and a proximal, GC-rich promoter region, which contained atypical CRE. Mutation of the CRE sequences dramatically reduced distal promoter activity; H89 inhibited IBMX-stimulated CREB phosphorylation and proximal and distal promoter activities. Distal promoter ...
Chromosomal translocations that fuse the mixed lineage leukemia (MLL) gene with multiple partners typify acute leukemias of infancy as well as therapy-related leukemias. We utilized a conditional knockin strategy to bypass the embryonic lethality caused by MLL-CBP expression and to assess the immediate effects of induced MLL-CBP expression on hematopoiesis. Within days of activating MLL-CBP, the fusion protein selectively expanded granulocyte/macrophage progenitors (GMP) and enhanced their self-renewal/proliferation. MLL-CBP altered the gene expression program of GMP, upregulating a subset of genes including Hox a9. Inhibition of Hox a9 expression by RNA interference demonstrated that MLL-CBP required Hox a9 for its enhanced cell expansion. Following exposure to sublethal γ-irradiation or N-ethyl-N-nitrosourea (ENU), MLL-CBP mice developed myelomonocytic hyperplasia and progressed to fatal myeloproliferative disorders. These represented the spectrum of therapy-induced acute myelomonocytic ...
Expression of CREBBP (CBP, KAT3A, RSTS, RTS) in cervix, uterine tissue. Antibody staining with HPA055861 and CAB004212 in immunohistochemistry.
The highly conserved, multifunctional Tudor-SN (also called SND1/p100) modulates gene expression by an assortment of processes in the nucleus and in the cytoplasm. The multifunctional properties of Tudor-SN can be attributed to its complex structure, consisting of multiple domains within the 4SN and Tsn modules (Gutierrez-Beltran et al., 2016). As a key component in RNA splicing, processing, and editing, the 4SN module has RNA-binding and RNA cleavage activities (Caudy et al., 2003; Scadden, 2005; Li et al., 2008). This modular region also exhibits unique protein recognition properties, as it interacts with several components of the basal transcription machinery, including ATP-dependent RNA helicase A (Välineva et al., 2006), Signal Transducer and Activator of Transcription6 (Yang et al., 2002), DNA-directed RNA polymerase II largest subunit, and CREB-binding protein (Välineva et al., 2005; Yang et al., 2007). A subsequent study also demonstrated that the SN-like domains bind to the 3′ UTR ...
Here, we present a structural and dynamic description of CBP-ID4 at atomic resolution. ID4 is the fourth intrinsically disordered linker of CREB-binding protein (CBP). In spite of the largely disordered nature of CBP-ID4, NMR chemical shifts and relaxation measurements show a significant degree of α-helix sampling in the protein regions encompassing residues 2-25 and 101-128 (1852-1875 and 1951-1978 in full-length CBP). Proline residues are uniformly distributed along the polypeptide, except for the two α-helical regions, indicating that they play an active role in modulating the structural features of this CBP fragment. The two helical regions are lacking known functional motifs, suggesting that they represent thus-far uncharacterized functional modules of CBP. This work provides insights into the functions of this protein linker that may exploit its plasticity to modulate the relative orientations of neighboring folded domains of CBP and fine-tune its interactions with a multitude of ...
Somatic mutations in CREBBP occur frequently in B-cell lymphoma. Here, we show that loss of CREBBP facilitates the development of germinal center (GC)-derived lymphomas in mice. In both human and murine lymphomas, CREBBP loss-of-function resulted in focal depletion of enhancer H3K27 acetylation and aberrant transcriptional silencing of genes that regulate B-cell signaling and immune responses, including class II MHC. Mechanistically, CREBBP-regulated enhancers are counter-regulated by the BCL6 transcriptional repressor in a complex with SMRT and HDAC3, which we found to bind extensively to MHC class II loci. HDAC3 loss-of-function rescued repression of these enhancers and corresponding genes, including MHC class II, and more profoundly suppressed CREBBP-mutant lymphomas in vitro and in vivo. Hence, CREBBP loss-of-function contributes to lymphomagenesis by enabling unopposed suppression of enhancers by BCL6/SMRT/HDAC3 complexes, suggesting HDAC3-targeted therapy as a precision approach for ...
The activation of the transcription factor NF-kB is central to the control of the cellular response triggered by many stimuli. Once released from the inhibitory molecule IkB, NF-kB is translocated to the nucleus, and it has to be phosphorylated to activate transcription. In protein kinase C (PKC)-deficient cells, NF-kB is transcriptionally inactive and the phosphorylation of the RelA subunit in response to tumor necrosis factor (TNF-alpha) is severely impaired. In vitro assays showed that PKC directly phosphorylates RelA. Here we demonstrate that Ser311 accounts for PKC phosphorylation of RelA and that this site is phosphorylated in vivo in response to TNF-alpha. Also, an inactivating mutation of that residue severely impairs RelA transcriptional activity, blocks its anti-apoptotic function and abrogates the interaction of RelA with the co-activator CBP as well as its recruitment, and that of RNA polymerase II (Pol II) with the interleukin-6 (IL-6) promoter. The interaction of endogenous CBP ...
Freds Dērsts piedzima Džeksonvilā, Floridā, ASV. Fredu audzināja viņa māte Anita, jo viņa bioloģiskais tēvs aizgāja no ģimenes, kad Fredam bija tikai dažas nedēļas. Freds ar māti dzīvoja ļoti trūcīgi, viņiem nebija ne mājas, ne darba, ne naudas. Viņi dzīvoja baznīcas bēniņos, ēdot bērnu pārtiku, ko nesa draudzes locekļi. Kad Fredam bija divi gadi, Anita iepazinās ar policijas oficieri Bilu, ar kuru drīz vien viņa apprecējās.. Fredam jaunībā patika tā pati mūzika, kas viņa vecākiem, un viņš bieži ākstījās dejojot un iztēlojoties, ka uzstājas publikas priekšā. Kad Freds paaugās, viņš un viņa pusbrālis Korijs (Bila un Anitas dēls) kļuva par Kiss faniem.. Vēlāk Freda ģimene pārvācās no Džeksonvillas, Floridā, uz Gastoniju, Ziemeļkarolīnā, kur Dērsts mācījās Hunter Huss vidusskolā.[1] Šeit viņš sāk aizrauties ar hiphopu un izveido breika dejošanas grupu Reckless Crew. Viņš sāk interesēties par kultūru, kas ir ...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
Vislielāko vīrusu daudzumu inficētie pacienti izdala 2.-3. slimības dienā, t.i., šajā periodā viņi ir visinfekciozākie, tomēr nelielos, inficēšanai pietiekamos daudzumos vīrusa izdale var saglabāties līdz pat 1-2 nedēļām.. Attiecībā uz sabiedrībā valdošo uzskatu, ka gaisa recirkulācijas sistēmas, piemēram, lidmašīnās, paaugstina saaukstēšanās risku, ASV 2002. gadā veiktais pētījums šādu faktu nav apstiprinājis, tāpēc šobrīd jādomā, ka pārneses ceļam ar nemazgātām rokām ir vislielākā nozīme saaukstēšanās vīrusu izplatībā.. Nereti tiek diskutēts par to, cik bieži gada laikā slimot ar saaukstēšanos ir normāli. Vidēji pirmsskolas vecuma bērni slimo 6-8 (līdz pat 12) reizes gadā, katru reizi slimībai ilgstot aptuveni 14 dienas, savukārt vecāki bērni un pieaugušie vidēji slimo 2-4 reizes gadā, katru reizi simptomiem ilgstot 5-7 dienas.. Smēķētājiem gan simptomi parasti ilgst vairāk par nedēļu hroniska elpceļu ...
May 2009). "Metformin and insulin suppress hepatic gluconeogenesis through phosphorylation of CREB binding protein". Cell. 137 ... Transport of PEP across the mitochondrial membrane is accomplished by dedicated transport proteins; however no such proteins ... it triggers phosphorylation of enzymes and regulatory proteins by Protein Kinase A (a cyclic AMP regulated kinase) resulting in ... In the liver, the FOX protein FoxO normally promotes gluconeogenesis in the fasted state, but insulin blocks Fox0 upon feeding ...
p90rsk also regulates transcription factors including cAMP response element-binding protein (CREB); estrogen receptor-α (ERα); ... Ribosomal+Protein+S6+Kinases,+90-kDa at the US National Library of Medicine Medical Subject Headings (MeSH) Ribosomal+Protein+ ... Erikson, Eleanor; Maller, James L. (1985). "A Protein Kinase from Xenopus Eggs Specific for Ribosomal Protein S6". PNAS. 82 (3 ... including other ribosomal proteins. Cytosolic substrates of p90rsk include protein phosphatase 1; glycogen synthase kinase 3 ( ...
"Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2" (PDF). Cellular Signalling. 27 (11): ... and protein molecules, and for protein engineering. Site-directed mutagenesis is one of the most important laboratory ... Commercial applications - Proteins may be engineered to produce mutant forms that are tailored for a specific application. For ... Site-directed mutagenesis is used to generate mutations that may produce a rationally designed protein that has improved or ...
"Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2" (PDF). Cell Signaling. 27 (11): 2252-60 ... See figure 3. of a recent study showing that CREB-binding protein is phosphorylated by HIPK2.) In plant physiology, ... Protein phosphorylation can be detected on an autoradiograph, after incubating the protein in vitro with the appropriate kinase ... The radiolabeled phosphate of latter is incorporated into the protein which is isolated via SDS-PAGE and visualized on an ...
"Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2" (PDF). Cellular Signalling. 27 (11): ... Green fluorescent protein (GFP) is also a photoprotein isolated and cloned from the jellyfish Aequorea victoria. Variants have ... activators or the different variants/fragments of the same activator are fused to the GAL4 DNA binding module at protein level ... This way the transcriptional activity of the different GAL4 fusion proteins can be directly compared using light as a readout. ...
CREB (cAMP response element binding protein) has been implicated in the upregulation of HULC. HULC RNA inhibits miR-372 ... or RNA-binding proteins (RBPs). Similarly, competition by ceRNAs for RNA-binding proteins has also been reported in eukaryotic ... The CD44 3'UTR has been shown to regulate expression of the CD44 protein and cell cycle regulation protein, CDC42, by ... transcriptomic miRNA-binding sites and (2) the impact miRNA concentrations have on the number of binding sites that must be ...
Transfection of N-cad/CTF2 decreases nuclear CBP (CREB-binding protein) and increases cytosolic CBP. Furthermore, N-cad/CTF2 co ... For example, the cadherin-α-catenin complex binds the actin cytoskeleton, though whether it binds via binding proteins or ... In homodimeric form, α-catenins do not bind β- catenins, but preferentially bind F-actin and other proteins promoting F-actin ... and contain both extracellular Ca2+ binding domains as well as intracellular domains for binding their protein partners. A ...
Mechanistically, signaling molecules phosphorylate cAMP-responsive element binding protein (CREB), which is a transcription ... cAMP-responsive element-binding (CREB) protein, a transcription factor, is also implicated in changes in synaptic plasticity. ... Inhibition of CREB translation likewise inhibited synaptic changes. CREB is activated in its phosphorylated form, acting as a ... CaMKII (Calcium-calmodulin-dependent protein kinase II) is a protein kinase that must be activated by calmodulin in order to ...
"Target Structure-Based Discovery of Small Molecules that Block Human p53 and CREB Binding Protein (CBP) Association". Chemistry ... Selectively Inhibiting Human Immunodeficiency Virus 1 Tat Association with the Human Coactivator p300/CREB Binding Protein- ... Qian C.; Zhang Q.; Li S.; Zeng L.; Walsh M.J.; Zhou M.-M. (2005). "Structure and Chromosomal DNA Binding of the SWIRM Domain". ... Yan K.S.; Yan S.; Farooq A.; Han A.; Zeng L.; Zhou M.-M. (2003). "Structure and Conserved RNA Binding of the PAZ Domain". ...
The transcription factor cAMP response element-binding protein (CREB) is a well-studied mechanism of neuronal memory allocation ... CREB may be activated by multiple pathways. For example, the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) ... and this is accompanied by a reduction in the transcription of genes modulated by the CREB protein. Metaplasticity is a term ... cyclic AMP responsive element-binding protein) in this process. Certain synapses on recruited neurons are more likely to ...
One of the nuclear targets for PKA is the transcriptional control protein CREB (cAMP response element binding protein). In ... Kandel identified CREB as being a protein involved in long-term memory storage. One result of CREB activation is an increase in ... Neurotransmitters, second messenger systems, protein kinases, ion channels, and transcription factors like CREB have been ... Kandel, Eric R. (May 14, 2012). "The molecular biology of memory: cAMP, PKA, CRE, CREB-1, CREB-2, and CPEB". Molecular Brain. 5 ...
Activators of CREB phosphorylation, for instance forskolin and KSHV binding to endothelial cell targets, can also enhance miR- ... In this instance, the target of translational suppression appears to be p300, a protein that associates with CREB and is an ... Vo N, Klein ME, Varlamova O, Keller DM, Yamamoto T, Goodman RH, Impey S (2005). "A cAMP-response element binding protein- ... The transcription of this cluster was found to be enhanced by the transcription factor CREB (cAMP-response element binding ...
... using a mechanism that activates the transcription factor cyclic adenosine monophosphate response element-binding protein (CREB ... Yang S, Alkayed NJ, Hurn PD, Kirsch JR (March 2009). "Cyclic adenosine monophosphate response element-binding protein ... 4-PPBP is a molecule which binds to sigma receptors. 4-PPBP decreases neuronal nitric oxide synthase (nNOS) activity and ... Neuroprotection that is associated with 4-PPBP increases Bcl-2 expression; Bcl-2 expression is regulated by CREB. 3-PPP Yang S ...
... monophosphate response element binding protein). According to the model proposed by Carlezon et al., use of cocaine increases ... This indicates that increasing CREB reverses the positive effects of cocaine. Northern blot analysis several days after CREB ... "Inhibition of cAMP response element-binding protein or dynorphin in the nucleus accumbens produces an antidepressant-like ... whereas overexpressing wild-type CREB caused an increase in depression-like symptoms. As described previously, CREB increases ...
This gene encodes CREB-binding protein, a protein that activates various transcription factors some of which are implicated in ... a protein that regulates cell proliferation and the CDKN2B gene which encodes multiple tumor suppressor 2, a protein that ... This gene encodes SET domain containing 2, a protein that acts to reduce the occurrence of gene deletions and tumor formation. ... TP53 encodes tumor suppressor p53, a protein that regulates cell proliferation, death, and tumor formation. Mutations in JAK- ...
The activated CREB binds along with CREB binding protein, co-activator to the CRE region of the DNA in the nucleus. CREB and ... When NMB binds to its 7-TMR, the heterotrimeric G protein that is attached to the receptor is activated. The G-protein is ... In the activated NMBR/G-protein complex, there occurs an exchange of GTP for GDP bound to G-α subunit. The G-α subunit, in turn ... cAMP activates of the enzyme Protein Kinase A (PKA). PKA enters the nucleus and activates the cAMP response element-binding ...
... transcription requires the protein kinase-A-mediated phosphorylation of the cAMP-response element-binding protein(CREB). ... "AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic ... Lee JA, Kim H, Lee YS, Kaang BK (2003). "Overexpression and RNA interference of Ap-cyclic AMP-response element binding protein- ... "Overexpression of and RNA interference with the CCAAT enhancer-binding protein on long-term facilitation of Aplysia sensory to ...
Histone acetyltransferase enzymes (HATs) such as CREB-binding protein also dissociate the DNA from the histone complex, ... or protein binding. In both prokaryotes and eukaryotes, a large number of RNA binding proteins exist, which often are directed ... Such 3'-UTRs often contain both binding sites for microRNAs (miRNAs) as well as for regulatory proteins. By binding to specific ... Trithorax-group protein, Polycomb-group protein, or any other DNA-binding element to which a good antibody is available. ...
When cAMP levels are increased in neurons, this may trigger activation of cAMP response element-binding proteins (CREB). CREBs ...
This gene is regulated by the cAMP response element-binding (CREB) protein pathway and is capable of enhancing dendritic growth ... Arc is a member of a class of proteins called immediate early genes (IEG) that are rapidly transcribed in response to synaptic ... It is the variety of proteins and effect of the signal that fundamentally lead to the plasticity feature. Activity-dependent ... This component of the neuron contains a variety of chemical messengers and proteins that allow for the transmission of ...
The CREBBP gene makes a protein that helps control the activity of many other genes. The protein, called CREB-binding protein, ... cells make only half of the normal amount of CREB binding protein. A reduction in the amount of this protein disrupts normal ... These mutations result in the loss of one copy of the gene in each cell, which reduces the amount of p300 protein by half. Some ... Although researchers do not know how a reduction in the amount of p300 protein leads to the specific features of Rubinstein- ...
This binding leads to an increase in calcium levels which in turn can activate the cAMP response element-binding protein (CREB ... This binding impacts the expression of proteins encoded by those mRNAs by either inducing the cleavage of the mRNA, ... This leads to hemimethylated DNA, where an older methylated strand is bound to a younger unmethylated one. DNMT1 is an enzyme ... This is relevant because Sp1 binds to GC-rich promoter regions which prevent the methylation of these regions during embryonic ...
... a novel p300/CREB-binding protein-specific inhibitor of acetyltransferase, represses the acetylation of histone/nonhistone ... protein) M. R. S. Rao India portal Biology portal His thesis won the Giri Memorial Award for best thesis in Biochemistry. ... Compounds that selectively bind to copper ions in a sample and methods of preparation and use - Patent No. 8642764. Missing or ... Zinc-Metalloprotein Nature of Rat Spermatidal Protein TP2 and its Interactions with DNA. After a short stint at the National ...
Another possible contributing factor are mutations in the CREB-binding protein, whose function includes activating ... Such gene encodes for the protein Rb responsible for stopping the cell cycle at the G1 phase. ...
SRCAP encodes a SNF2-related chromatin-remodeling ATPase that is a coactivator for CREB-binding protein (or CBP), which is the ... This disrupted interaction between the proteins most likely explains the clinical overlap between FHS and RTS. SRCAP has been ... February 2012). "Mutations in SRCAP, encoding SNF2-related CREBBP activator protein, cause Floating-Harbor syndrome". American ... and three C-terminal AT-hook DNA-binding motifs). The CBP interaction domain of SRCAP is located centrally. Thus, the mechanism ...
... especially transcription factor cAMP response element binding protein (CREB). cAMP/PKA/CREB axis mediates the inhibition of TNF ... The latent complex is covalently linked to the extracellular matrix by binding to latent TGF-β-binding proteins. TGF-β is ... αvβ6 integrin is able to mediate activation of TGF-β by binding to TGF-β1 LAP, which serves as a ligand binding site for the ... on iron-binding proteins, and on the synthesis of toxic cationic polypeptides. Macrophages possess a repertoire of ...
Upon calcium influx, a cAMP-response element binding protein (CREB) is phosphorylated, altering the potassium concentrations ... or binding to calcium-binding proteins such as parvalbumin and calretinin. Retrograde signaling is necessary in the calyx of ... Eph proteins also ensure that while axons pass through the ipsilateral MNTB, branching and final termination of these ... Additionally, Eph proteins are integral for further auditory circuit system development after initial embryonic calyx formation ...
CREB, a cAMP response element-binding protein and transcriptional activator, binds CBP forming the CREB: CBP complex. This ... such as the CREB binding protein (which has HAT activity). By observing the rates of acetylation in the insular cortex ... Mice that have genetic disruptions for CpG binding protein 2 (MeCP2) have been shown to have significant problems in ... such as Rett syndrome.Rubinstein-Tabyi syndrome causes mental retardation through possible mutations in CREB-binding protein ...
... "cAMP response element binding protein" (CREB). The nucleus accumbens (NAcc) is one brain structure that has been implicated in ... In addition to CREB, it is hypothesized that stress mechanisms play a role in dependence. Koob and Kreek have hypothesized that ... In the NAcc, CREB is activated by cyclic adenosine monophosphate (cAMP) immediately after a high and triggers changes in gene ... A sustained activation of CREB thus forces a larger dose to be taken to reach the same effect. In addition, it leaves the user ...
Jin Y, Zeng SX, Dai MS, Yang XJ, Lu H (August 2002). "MDM2 inhibits PCAF (p300/CREB-binding protein-associated factor)-mediated ... protein binding. • enzyme binding. • metal ion binding. • identical protein binding. • ubiquitin protein ligase binding. • p53 ... 5S rRNA binding. • zinc ion binding. • ribonucleoprotein complex binding. • protein N-terminus binding. • ubiquitin protein ... disordered domain specific binding. • protein domain specific binding. • receptor serine/threonine kinase binding. • peroxisome ...
RNA polymerase II regulatory region sequence-specific DNA binding. • DNA binding. • sequence-specific DNA binding. • ... Homeobox protein Hox-D8 is a protein that in humans is encoded by the HOXD8 gene.[5][6][7] ... 1989). "Complementary homeo protein gradients in developing limb buds". Genes Dev. 3 (5): 641-50. doi:10.1101/gad.3.5.641. PMID ... HOXD8+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) ...
protein homodimerization activity. · sequence-specific DNA binding. · metal ion binding. · protein heterodimerization activity ... C/EBP(α、β、γ、δ、ε、ζ) · CREB(1、3、L1) · CREM · DBP · DDIT3 · GABPA · HLF · MAF(B、F、G、K) · NFE(2、L1、L2、L3) · NFIL3 · NRL · NRF(1、2、3 ... DNA binding. · chromatin binding. · double-stranded DNA binding. · sequence-specific DNA binding transcription factor activity ... Rb · RBL1(英语:Retinoblastoma-like protein 1) · RBL2(英语:Retinoblastoma-like protein 2) ...
receptor binding. • связывание с белками плазмы. • гомодимеризация белка. • cell adhesion molecule binding. • protein binding ... positive regulation of CREB transcription factor activity. • heterotypic cell-cell adhesion. • positive regulation of ... positive regulation of protein-glutamine gamma-glutamyltransferase activity. • regulation of neuroinflammatory response. • ... Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins ...
receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ... Tao X, Finkbeiner S, Arnold DB, Shaywitz AJ, Greenberg ME (April 1998). "Ca2+ influx regulates BDNF transcription by a CREB ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... positive regulation of receptor binding. • regulation of protein localization to cell surface. • regulation of receptor ...
Blackwood EM, Eisenman RN (1991). "Max: a helix-loop-helix zipper protein that forms a sequence-specific DNA-binding complex ... CREB (1, 3, L1) • CREM • DBP • DDIT3 • GABPA • HLF • MAF (B, F, G, K) • NFE (2, L1, L2) • NFIL3 • NRL • NRF1 • XBP1 ... "Control of c-myc mRNA half-life in vitro by a protein capable of binding to a coding region stability determinant". Genes Dev. ... 1988). "A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is ...
Rouaux C, Loeffler JP, Boutillier AL (September 2004). "Targeting CREB-binding protein (CBP) loss of function as a therapeutic ... "Sodium valproate exerts neuroprotective effects in vivo through CREB-binding protein-dependent mechanisms but does not improve ... The UBQLN2 gene encodes the protein ubiquilin 2 which is responsible for controlling the degradation of ubiquitinated proteins ... As that gene's name suggests, BACE1 is an enzymatic protein that cleaves the Amyloid Precursor Protein into the insoluble ...
One of the nuclear targets for PKA is the transcriptional control protein CREB (cAMP response element binding protein).[5] In ... Kandel identified CREB as being a protein involved in long-term memory storage. One result of CREB activation is an increase in ... Neurotransmitters, second messenger systems, protein kinases, ion channels, and transcription factors like CREB have been ... By 1980, collaboration with Paul Greengard resulted in demonstration that cAMP-dependent protein kinase, also known as protein ...
sequence-specific DNA binding. • DNA binding. • transcription factor binding. • protein domain specific binding. • RNA ... HNF-3G is a member of the forkheadclass of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional ... 2003). "Transcriptional regulation of human CYP3A4 basal expression by CCAAT enhancer-binding protein alpha and hepatocyte ... transcription factor activity, sequence-specific DNA binding. • transcription regulatory region DNA binding. • ...
... towards the transcriptional control of AMPA receptors in longer-term memory through cAMP response element-binding protein (CREB ... All AMPARs contain PDZ-binding domains, but which PDZ domain they bind to differs. For example, GluA1 binds to SAP97 through ... "Synaptic anchorage of AMPA receptors by cadherins through neural plakophilin-related arm protein AMPA receptor-binding protein ... Of note, AMPARs cannot directly bind to the common synaptic protein PSD-95 owing to incompatible PDZ domains, although they do ...
Protein kodiran ovim genom je član NR1 potfamilije nuklearnih hormonskih receptora. To je DNA-vezujući protein koji se može ... CREB (1, 3, L1) • CREM • DBP • DDIT3 • GABPA • HLF • MAF (B, F, G, K) • NFE (2, L1, L2) • NFIL3 • NRL • NRF1 • XBP1 ... 2001). "X-ray structure of the orphan nuclear receptor RORbeta ligand-binding domain in the active conformation.". Embo J. 20 ( ... 2000). "Differential ligand-dependent protein-protein interactions between nuclear receptors and a neuronal-specific cofactor ...
... and it is the last protein to bind in the complex.. Histones tend to be positively charged proteins with N-terminal tails that ... CREB, c-Jun, C/EBPβ, NF-E2, SREBP, IRF2, Sp3, YY1, KLF13, EVI1, BCL6, HNF-4, ER81, and FOXO4 (AFX).[18] ... structural proteins, polyamines, and proteins involved in nuclear import.[3] Acetylation of these proteins can alter their ... Many MYST proteins also contain a cysteine-rich, zinc-binding domain within the HAT region in addition to an N-terminal ...
Sterol regulatory element-binding proteins (SREBPs) are transcription factors that bind to the sterol regulatory element DNA ... Sterol regulatory element-binding transcription factor 1. X-ray crystallography of Sterol Regulatory Element Binding Protein 1A ... SCAP, in turn, can bind reversibly with another ER-resident membrane protein, INSIG. In the presence of sterols, which bind to ... proteins. However, in contrast to E-box-binding HLH proteins, an arginine residue is replaced with tyrosine making them capable ...
bile acid binding. • protein binding. • ligand-dependent nuclear receptor binding. • transcription factor activity, RNA ... RNA polymerase II distal enhancer sequence-specific DNA binding. • sequence-specific DNA binding. • DNA binding. • ... metal ion binding. • retinoid X receptor binding. • steroid hormone receptor activity. • transcriptional activator activity, ... chenodeoxycholic acid binding. • transcription regulatory region sequence-specific DNA binding. • RNA polymerase II regulatory ...
Mitchell PJ, Tjian R (1989). „Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins". Science ... CREB (1, 3, L1) • CREM • DBP • DDIT3 • GABPA • HLF • MAF (B, F, G, K) • NFE (2, L1, L2) • NFIL3 • NRL • NRF1 • XBP1 ... Lee TI, Young RA (2000). „Transcription of eukaryotic protein-coding genes". Annu. Rev. Genet. 34: 77-137. PMID 11092823. doi: ... U molekularnoj biologiji i genetici, transkripcioni faktor (za sekvencu-specifični DNK-vezujući faktor) je protein koji se ...
... p38 mitogen-activated protein kinases (p38 Mpk), and cAMP response element-binding protein (CREB) which when activated ... G protein linkage[2]. pathways[2] Prostaglandin DP1 receptor. DP1. PGD2,,PGE2,PGF2α,PGI2=TXA2[6]. relaxant. Gs alpha subunit. ... stimulates PLC, IP3, PKC, ERK, p38 Mpk, and CREB Prostaglandin EP2 receptor. EP2. PGE2,PGF2α=PGI2,PGD2=TXA2[9]. relaxant. Gs ... stimulates AC, PKA, PI3K, AKT, ERK, p38 Mpk, & CREB; raises cAMP Prostaglandin F2α receptor. FP. PGF2α,PGD2,PGE2,PGI2=TXA2[12] ...
Acetylation of interferon regulatory factor-7 by p300/CREB-binding protein (CBP)-associated factor (PCAF) impairs its DNA ... RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity. • DNA binding. ... Morin P, Bragança J, Bandu MT, Lin R, Hiscott J, Doly J, Civas A (2002). «Preferential binding sites for interferon regulatory ... does not require double-stranded RNA-dependent protein kinase R or Ikappa B kinase but is blocked by Vaccinia virus E3L protein ...
protein binding. • RNA polymerase II core promoter proximal region sequence-specific DNA binding. • chromatin binding. • ... DNA binding. • p53 binding. • DNA binding, bending. • RNA polymerase II transcription factor binding. • chromatin DNA binding. ... zinc ion binding. • metal ion binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. • RNA ... core promoter binding. • transcription regulatory region sequence-specific DNA binding. • sequence-specific DNA binding. • RNA ...
MYB factors represent a family of proteins that include the conserved MYB DNA-binding domain. Plants contain a MYB-protein ... "Molecular cloning reveals that the p160 Myb-binding protein is a novel, predominantly nucleolar protein which may play a role ... Myb proto-oncogene protein also known as transcriptional activator Myb is a protein that in humans is encoded by the MYB gene.[ ... The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C- ...
DNA binding. • sequence-specific DNA binding. • protein domain specific binding. • transcription factor activity, sequence- ... Forkhead box protein A2 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are ... "ErbB3 binding protein 1 represses metastasis-promoting gene anterior gradient protein 2 in prostate cancer". Cancer Res. 70 (1 ... transcription factor binding. • RNA polymerase II core promoter proximal region sequence-specific DNA binding. • transcription ...
The cAMP response element-binding protein (CREB) is a transcription factor which is believed to be important in consolidating ... This protein is an autonomously active form of the enzyme protein kinase C (PKC), known as PKMζ. PKMζ maintains the activity- ... Yin, Jerry CP; Tully, Timothy (1996). "CREB and the formation of long-term memory". Current Opinion in Neurobiology. 6 (2): 264 ... Long-term memory, unlike short-term memory, is dependent upon the synthesis of new proteins.[35] This occurs within the ...
Calcium binds to proteins such as calmodulin (CaM) and an eye-specific protein kinase C (PKC) known as InaC. These proteins ... protein kinase A, and a transcription factor known as CREB. These molecules were shown to be also involved in synaptic ... InaD contains five binding domains called PDZ domain proteins, which specifically bind the C termini of target proteins. ... In addition, proteins called arrestins bind metarhodopsin and prevent it from activating more Gq. A sodium-calcium exchanger ...
In protein synthesis, PKA first directly activates CREB, which binds the cAMP response element, altering the transcription and ... The dimerization and docking (D/D) domain of the dimer binds to the A-kinase binding (AKB) domain of A-kinase anchor protein ( ... Protein Kinase A is directed to specific sub cellular locations after tethering to Protein kinase A anchoring proteins (AKAPs ... The mechanisms of further effects may be divided into direct protein phosphorylation and protein synthesis: *In direct protein ...
McKay DB, Steitz TA (1981). "Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed ... CREB (1, 3, L1) • CREM • DBP • DDIT3 • GABPA • HLF • MAF (B, F, G, K) • NFE (2, L1, L2) • NFIL3 • NRL • NRF1 • XBP1 ... Brennan RG, Matthews BW (1989). "The helix-turn-helix DNA binding motif.". J Biol Chem 264 (4): 1903-6. PMID 2644244. ... Pabo CO, Lewis M (1982). "The operator-binding domain of lambda repressor: structure and DNA recognition.". Nature 298 (5873): ...
protein binding. • neuropeptide hormone activity. • peptide hormone receptor binding. Cellular component. • extracellular ... The PKA then activates other intracellular signaling pathways like the phosphorylation of CREB and other transcriptional ... When VIP binds to VPAC2 receptors, a G-alpha-mediated signalling cascade is triggered. In a number of systems, VIP binding ... positive regulation of protein catabolic process. • regulation of protein localization. • antimicrobial humoral immune response ...
... binds and inhibits the CREB binding protein and p300 histone acetyltransferease activities on a repressed gene target, cyclin ... Nascent long ncRNAs have been shown to increase the activity of CREB binding protein, which in turn increases the transcription ... In the broad sense, this mechanism allows the cell to harness RNA-binding proteins, which make up one of the largest classes ... July 2008). "Induced ncRNAs allosterically modify RNA-binding proteins in cis to inhibit transcription". Nature. 454 (7200): ...
transcription factor binding. • activating transcription factor binding. • E-box binding. • protein binding. • protein ... DNA binding. • sequence-specific DNA binding. • RNA polymerase II regulatory region sequence-specific DNA binding. • protein ... transcription regulatory region DNA binding. Cellular component. • transcription factor complex. • protein complex. • nuclear ... Heart- and neural crest derivatives-expressed protein 2 is a protein that in humans is encoded by the HAND2 gene.[5][6] ...
a b C. S. Breivogel, G. Griffin, V. Di Marzo, B. R. Martin: Evidence for a new G protein-coupled cannabinoid receptor in mouse ... Influence of the N-1 alkyl chain length of cannabimimetic indoles upon CB1 and CB2 receptor binding. . In: Drug and Alcohol ... Reduced expression of glutamate receptors and phosphorylation of CREB are responsible for in vivo Δ9-THC exposure-impaired ... Der CNR1 wird auf dem Chromosomenabschnitt 6q14-q15 kodiert und wird in ein 472 Aminosäuren großes Protein transkribiert. Es ...
"Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2". Cellular Signalling. 27 (11): 2252-60 ... Commercial applications - Proteins may be engineered to produce mutant forms that are tailored for a specific application. For ... Investigative tools - specific mutations in DNA allow the function and properties of a DNA sequence or a protein to be ... This approach has been used to uncover the phosphorylation of the protein CBP by the kinase HIPK2 [22] ...
Regulatory motifs for CREB-binding protein and Nfe2l2 transcription factors in the upstream enhancer of the mitochondrial ... A new polymorphic site located in the human UCP1 gene controls the in vitro binding of CREB-like factor. . In: Int. J. Obes. ... Thermogenin (auch entkoppelndes Protein, engl. Uncoupling Protein 1 bzw. UCP1 genannt[1]) ist ein Transmembranprotein, das sich ... The uncoupling protein homologues: UCP1, UCP2, UCP3, StUCP and AtUCP. . In: Biochem. J.. . 345 Pt 2, 2000, S. 161-79. PMID ...
We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their ... InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites ...
CREB-binding protein, also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene. The CREB protein ... "Histone binding protein RbAp48 interacts with a complex of CREB binding protein and phosphorylated CREB". Molecular and ... CREB-binding protein has been shown to interact with: TF2, AR, AIRE, BRCA1, C-jun, CSK, Ccaat-enhancer-binding proteins, CDX2, ... First isolated as a nuclear protein [Ref] that binds to cAMP-response element-binding protein (CREB), this gene is now known to ...
CREB- binding protein;. CREB,. cAMP response element binding protein;. GST,. glutathione S-transferase;. CAT,. chloramphenicol ... CREB-binding protein (CBP) is a coactivator that interacts with the cAMP-response element binding protein (CREB), a process ... CREB-binding protein/p300 are transcriptional coactivators of p65. Mary E. Gerritsen, Amy J. Williams, Andrew S. Neish, Sarah ... CREB-binding protein/p300 are transcriptional coactivators of p65. Mary E. Gerritsen, Amy J. Williams, Andrew S. Neish, Sarah ...
Here we report that CREB phosphorylated by protein kinase A binds specifically to a nuclear protein of M(r) 265K which we term ... for CREB-binding protein). Fusion of a heterologous DNA-binding domain to the amino terminus of CBP enables the chimaeric ... Phosphorylated CREB binds specifically to the nuclear protein CBP.. Chrivia JC1, Kwok RP, Lamb N, Hagiwara M, Montminy MR, ... Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation ...
PREDICTED: CREB-binding protein-like [Danio rerio] PREDICTED: CREB-binding protein-like [Danio rerio]. gi,528473282,ref,XP_ ... PREDICTED: CREB-binding protein-like [Danio rerio]. NCBI Reference Sequence: XP_005163780.1 ... The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in ... The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in ...
2003). Intrathecal injection of cAMP response element binding protein (CREB) antisense. By Wise Young in forum Neuropathic Pain ... CREB-Binding Protein This sounds promising for the future....Wonder if exercise builds it up?. https://www.eurekalert.org/pub_ ... TAR DNA-binding protein 43 in neurodegenerative disease.. By wildwilly in forum Neurodegeneration Research ... 2001). RNA-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its ... acetyl-CoA + L-lysyl-[protein] = CoA + H+ + N6-acetyl-L-lysyl-[protein] UniProt ... This protein in other organisms (by gene name): Q4LE28 - Homo sapiens 0 * Q6GQV9 - Mus musculus no matching PDB entries ...
Drosophila CREB binding protein (CBP), which is encoded by the nejire (nej) locus, belongs to the CBP/p300 family of proteins ( ... and Dach proteins mediated through CREB binding protein. Mol. Cell. Biol. 22: 6759-6766. ... CREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila. Justin P. Kumar, Tazeen Jamal, Alex ... CREB Binding Protein Functions During Successive Stages of Eye Development in Drosophila. Justin P. Kumar, Tazeen Jamal, Alex ...
Biochemical and NMR Mapping of the Interface between CREB-binding Protein and Ligand Binding Domains of Nuclear Receptor: ... The binding protein of cAMP response element binding protein (CBP) appears to be a promiscuous coactivator for an increasing ... a Transcriptional Activator That Interacts with Multiple Domains of cAMP-responsive Element-binding Protein (CREB)-binding ... CREB Binding Protein Is a Coactivator for the Androgen Receptor and Mediates Cross-talk with AP-1 ...
Our results indicate that Nrf2 exploits the cooperative binding of two independent transactivation domains to CBP in the ... Neh4 and Neh5 both individually and cooperatively bind to CBP (CREB (cAMP Responsive Element Binding protein) Binding Protein ... Two domains of Nrf2 cooperatively bind CBP, a CREB binding protein, and synergistically activate transcription Genes Cells. ... Importantly, the CBP-binding activity of Nrf2 deletion mutants positively correlated with their transactivation activity. Neh5 ...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
Gerritsen, M.E., Williams, A.J., Neish, A.S., Moore, S., Shi, Y. and Collins, T. (1997) CREBbinding protein/p300 are ... von Mikecz, A., Zhang, S., Montminy, M., Tan, E.M. and Hemmerich, P. (2000) CREBbinding protein (CBP)/p300 and RNA polymerase ... Prothymosin α interacts with the CREBbinding protein and potentiates transcription. Zoe Karetsou, Adroniki Kretsovali, Carol ... We report here that ProTα physically interacts with the CREBbinding protein (CBP), which is a versatile transcription co‐ ...
We have previously shown that the transcriptional cofactor CREB-binding protein (CBP) binds to the zinc finger domain of GATA-1 ... CREB-Binding Protein Acetylates Hematopoietic Transcription Factor GATA-1 at Functionally Important Sites. Hsiao-Ling Hung, ... 1996) CREB-binding protein activates transcription through multiple domains. J. Biol. Chem. 271:28138-28145. ... 1998) CREB-binding protein (CBP) cooperates with transcription factor GATA-1 and is required for erythroid differentiation. ...
The CREB-binding protein(CBP) is a transcriptional co-activators of various sequence-specific DNA-binding transcription factors ... Immunohistochemical Expression of p53 Protein and CREB-binding Protein in Polyps and Adenocarcinomas of Colon. ... RESULTS: 1. p53 protein expression was observed in 15% (9/60) of hyperplastic polyps, in 68.9% (124/180) of adenomatous polyps ... This article examined the expression levels of the p53 protein and CBP as well as their diagnostic value in a biopsy sample. ...
Normal P53 Function Requires An Association With The CREB Binding ... Are Conferred Primarily Through The Binding And Inactivation Of ... Acetylation, Animals, Antigens, Viral, Tumor, CREB-Binding Protein, Cell Line, Humans, Mice, Phosphorylation, Protein Binding, ... Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53.. Borger DR, DeCaprio JA ... Normal p53 function requires an association with the CREB binding protein (CBP)/p300 coactivators, and a ternary complex ...
... cAMP Response Element Binding Protein)(48H2), Artikelnummer: C7915-25.100 von United States Biological bei Biomol kaufen! ... Produktinformationen "Anti-CREB (cAMP Response Element Binding Protein)(48H2)" CREB is a bZIP transcription factor that ... While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a ... Additionally, CREB signaling is involved in learning and memory in several organisms. CREB is able to selectively activate ...
1998) cAMP-response-element-binding-protein-binding protein (CBP) and p300 are transcriptional co-activators of early growth ... we show that LPS-mediated TNF-α transcription is dependent upon CREB binding protein (CBP) and p300 coactivator proteins and, ... assembly of the LPS-stimulated TNF-α enhancer complex is dependent upon the coactivator proteins CREB binding protein and p300 ... 1993) Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature 365:855-859. ...
GST-Tat was also used in binding studies with other proteins, including RelA and Stat2. Neither of these proteins bound to GST- ... 1993) Phosphorylated CREB binds specifically to the nuclear protein CBP. Nature 365:855-859. ... Interaction of Human Immunodeficiency Virus Type 1 Tat with the Transcriptional Coactivators p300 and CREB Binding Protein. ... Among the factors associated with the basal transcription complex, p300 and the related CREB binding protein (CBP) (2) have ...
... the authors found that the primers listed for CREB-binding protein were not correct. This mistake occurred during assembly of ... Multiple functions of CREB-binding protein during postembryonic development: identification of target genes. BMC Genomics. 2017 ... Roy, A., George, S. & Palli, S.R. Correction to: Multiple functions of CREB-binding protein during postembryonic development: ... Correction to: Multiple functions of CREB-binding protein during postembryonic development: identification of target genes. * ...
Here we report that CREB is present in the mitochondrial matrix of neurons and that it binds directly to cyclic AMP response ... underlies the protective effects of CREB and raise the possibility that decreased mitochondrial CREB activity contributes to ... Disruption of CREB activity in the mitochondria decreases the expression of a subset of mitochondrial genes, including the ND5 ... These results demonstrate that regulation of mitochondrial gene expression by mitochondrial CREB, in part, ...
... the transcriptional coactivator p300 and the closely related CREB-binding protein (CBP), having histone acetyltransferase (HAT ... The HIV-1 Tat protein binds to a stem-loop structure at the 5 end of viral mRNA and relieves this inhibition by inducing a ... Tat associates with HAT activity in human nuclear extracts and binds to p300 and CBP both in vitro and in vivo. Integrity of ... the integrated viral promoter is present in a chromatin-bound conformation and is transcriptionally silent in the absence of ...
CREB-Binding Protein; Fungal Proteins/genetics/metabolism; Nuclear Proteins/*chemistry/genetics/*metabolism; Trans-Activators/* ... Recombinant Fusion Proteins/metabolism; Proto-Oncogene Proteins/*metabolism; DNA/metabolism; DNA-Binding Proteins/metabolism; * ... Induction-independent recruitment of CREB-binding protein to the c-fos serum response element through interactions between the ... Induction-independent recruitment of CREB-binding protein to the c-fos serum response element through interactions between the ...
Creb-binding protein (CBP). Using GST pull-down assays, we demonstrate that MIER1 interacts with CBP and that this interaction ... Two major protein isoforms have been identified, MIER1α and β, which differ in their C-terminal sequence. Previously, we ... Histone 4 peptide binding assays demonstrate that this inhibition of HAT activity is not the result of interference with ... Functional analysis, using HEK293 cells, shows that the CBP bound to MIER1 in vivo has no detectable HAT activity. ...
CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including ... Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins ... CREB-binding protein (CBP) is a universal transcriptional co-regulator. It controls the expression of several genes including ... Multiple Functions of CREB-Binding Protein During Postembryonic Development: Identification of Target Genes ...
... cAMP response element-binding protein - CREB Polyclonal \ 18-662-20054 for more molecular products just contact us ... cAMP response element-binding protein - CREB Polyclonal. Related products : cAMP response element-binding protein - CREB ... cAMP response element-binding protein - CREB Polyclonal / Product Detail : 18-662-20054 cAMP response element-binding protein ... 12956721] Cyclic AMP response element-binding (CREB)-like proteins in a molluscan brain: cellular localization and learning- ...
Prothymosin α interacts with the CREBbinding protein and potentiates transcription. Zoe Karetsou, Adroniki Kretsovali, Carol ... ProTα binds to CBP in vivo and in vitro. (A) CBP colocalizes with ProTα. HeLa cells were double‐labeled with the polyclonal ... D) Identification of the CBP‐binding site within the ProTα molecule. ProTα (2 μg) was incubated with GST (lane 1), or the CBP ... PTα corresponds to 0.1 μg of purified protein. Detection was performed by ECL. The upper panel shows Coomassie Blue staining of ...
MOUSE CREB-binding protein gi,481698,pir,,S39161 CREB-binding protein - mouse gi,435855,gb,AAB28651.1, CREB-binding protein; ... CREB binding protein [Homo sapiens] gi,3023440,sp,Q92793,CBP_HUMAN CREB-binding protein gi,2443859,gb,AAC51770.1, CREB-binding ... gi,19547885,gb,AAL87531.1, CREB-binding protein [Mus musculus] gi,19547887,gb,AAL87532.1, CREB-binding protein [Mus musculus] ... CREB-binding protein like fami... 114 1e-24 gi,17554044,ref,NP_499201.1, CREB-binding protein like fami... 79 5e-14 gi,482238, ...
MOUSE CREB-binding protein gi,481698,pir,,S39161 CREB-binding protein - mouse gi,435855,gb,AAB28651.1, CREB-binding protein; ... CREB binding protein [Homo sapiens] gi,3023440,sp,Q92793,CBP_HUMAN CREB-binding protein gi,2443859,gb,AAC51770.1, CREB-binding ... gi,19547885,gb,AAL87531.1, CREB-binding protein [Mus musculus] gi,19547887,gb,AAL87532.1, CREB-binding protein [Mus musculus] ... CREB-binding protein like fami... 81 2e-14 gi,17551090,ref,NP_510006.1, CREB-binding protein like fami... 81 3e-14 gi,17508323, ...
Non-enzymatic BRD containing proteins. Detailed annotation on the structure, function, physiology, pharmacology and clinical ... 2011) A small molecule binding to the coactivator CREB-binding protein blocks apoptosis in cardiomyocytes. Chem. Biol., 18 (4 ... Non-enzymatic BRD containing proteins: CREB binding protein. Last modified on 12/07/2018. Accessed on 04/12/2020. IUPHAR/BPS ... 2006) Target structure-based discovery of small molecules that block human p53 and CREB binding protein association. Chem. Biol ...
Here we show that CREB and the highly related protein ATF-1 are also potent transcription inhibitors. Strikingly, CREB inhibits ... Inhibition requires that the CREB dimerization and DNA-binding domains are intact. However, inhibition is not dependent upon ... The ability of an activator protein to inhibit transcription in such a promiscuous fashion has not been previously reported. ... CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP ...
  • A small molecule inhibitor (I-CBP112) binding to the bromodomain domain of CBP/p300 has been developed for leukemia therapy. (wikipedia.org)
  • CBP (CREB-binding protein) and p300 are versatile coactivators that link transcriptional activators to the basal transcriptional apparatus. (pnas.org)
  • Direct physical interactions of CBP/p300 with p65 were demonstrated by glutathione S -transferase fusion protein binding, and coimmunoprecipitation/Western blot studies. (pnas.org)
  • The adenovirus E1A 12S protein, which complexes with CBP and p300, inhibited p65-dependent gene expression. (pnas.org)
  • Members of the CREB binding protein (CBP)/p300 family have been shown to influence development by (1) acting as bridging molecules between the basal transcriptional machinery and specific DNA-binding transcription factors, (2) physically interacting with terminal members of signaling cascades, (3) acting as transcriptional coactivators of downstream target genes, and (4) playing a key role in chromatin remodeling. (genetics.org)
  • Finally, CBP and p300 were recently shown to acetylate nonhistone proteins such as the tumor suppressor protein p53 ( 16 ), the erythroid Kruppel-like factor (EKLF) ( 48 ), and the basal transcription factors, TFIIE and TFIIF ( 19 ). (asm.org)
  • Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53. (thebiogrid.org)
  • Normal p53 function requires an association with the CREB binding protein (CBP)/p300 coactivators, and a ternary complex containing SV40 LT, p53, and CBP/p300 has been identified previously. (thebiogrid.org)
  • In this report, we have evaluated a secondary function of p53 bound to the SV40 LT complex in mediating the binding of human CBP/p300. (thebiogrid.org)
  • We demonstrate that p53 associated with SV40 LT was posttranslationally modified in a manner consistent with the binding of CBP/p300. (thebiogrid.org)
  • Therefore, the binding of p53 serves to modify SV40 LT by targeting CBP and p300 binding to direct the acetylation of SV40 LT. (thebiogrid.org)
  • Furthermore, assembly of the LPS-stimulated TNF-α enhancer complex is dependent upon the coactivator proteins CREB binding protein and p300. (asm.org)
  • Furthermore, we show that LPS-mediated TNF-α transcription is dependent upon CREB binding protein (CBP) and p300 coactivator proteins and, moreover, that the intrinsic transcriptional activity of CBP and p300 is potentiated by LPS. (asm.org)
  • Thus, a unique TNF-α enhancer complex, including Ets, Elk-1, Sp1, ATF-2-Jun, and the coactivator proteins CBP and p300, is assembled on the TNF-α promoter in LPS-stimulated monocytes. (asm.org)
  • Here we demonstrate that transcriptional activation by HIV-1 Tat involves p300 or the related cellular transcriptional coactivator CREB binding protein (CBP). (asm.org)
  • The carboxy-terminal region of p300, which binds to E1A, was shown to bind specifically to the highly conserved basic domain of Tat, which also mediates binding to the Tat-responsive region RNA stem-loop structure. (asm.org)
  • Among the factors associated with the basal transcription complex, p300 and the related CREB binding protein (CBP) ( 2 ) have emerged as coactivators for a broad group of cellular transcription factors (for reviews, see references 5 and 34 ). (asm.org)
  • HIV-1 tat transactivator recruits p300 and CREB-binding protein histone acetyltransferases to the viral promoter. (semanticscholar.org)
  • A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. (childrensmercy.org)
  • Finally, we report that the formation of E2F1-p300/CREB-binding protein-associated factor (P/CAF) complexes is preferentially induced in doxorubicin-treated cells, and that P/CAF acetyltransferase (HAT), but not p300 HAT activity, is required for a significant E2F1 stabilization and accumulation. (elsevier.com)
  • Phosphorylation of ETS transcription factor ER81 in a complex with its coactivators CREB-binding protein and p300. (edu.sa)
  • We have previously shown that CREB-binding protein (CBP/p300) act as an important SOX9 co-activator during chondrogenesis. (elsevier.com)
  • Physical and functional interactions among AP-2 transcription factors, p300/CREB-binding protein, and CITED2. (ox.ac.uk)
  • The transcriptional co-activators and histone acetyltransferases p300/CREB-binding protein (CBP) interact with CITED2, a transcription factor AP-2 (TFAP2) co-activator. (ox.ac.uk)
  • These findings demonstrated that HNF-1α binds to the 5′-untranslated region of GLUT2 and that p300 acts as a transcriptional co-activator for HNF-1α. (diabetesjournals.org)
  • The activity of various DNA-binding transcription factors is regulated by global transcriptional co-activators such as p300 and CREB-binding protein (CBP). (diabetesjournals.org)
  • p300/CBP binds not only to the activation domain of some transcription factors, such as CREB and the glucocorticoid receptor, but also to multiple components of the basal transcriptional machinery, including the TFIIB ( 15 ) and RNA polymerase II holoenzyme complex ( 17 ), suggesting that p300/CBP serves as a molecular bridge between the transcription factors and the basal transcriptional machinery. (diabetesjournals.org)
  • For example, CBP and p300 binding to RELA are enhanced when serine 276 or 311 is phosphorylated. (wikipedia.org)
  • Human CREB-binding protein/p300-interacting transactivator with ED-rich tail (CITED) 4, a new member of the CITED family, functions as a co-activator for transcription factor AP-2. (ox.ac.uk)
  • Members of the CREB-binding protein/p300-interacting transactivator with ED-rich tail (CITED) family bind CREB-binding protein and p300 with high affinity and regulate gene transcription. (ox.ac.uk)
  • CITED4 and p300/CREB-binding protein are present in endogenous naturally occurring complexes, indicating that they interact physiologically. (ox.ac.uk)
  • The cAMP response element binding protein (CREB)-binding protein (CBP)/p300 family of coactivator proteins regulates gene transcription through the integration of multiple signal transduction pathways. (duke.edu)
  • Ionov, Y, Matsui, SI & Cowell, JK 2004, ' A role for p300/CREB binding protein genes in promoting cancer progression in colon cancer cell lines with microsatellite instability ', Proceedings of the National Academy of Sciences of the United States of America , vol. 101, no. 5, pp. 1273-1278. (elsevier.com)
  • p/ CIP stands for p300/ CREB-binding protein (CBP)-interacting protein. (acronymsandslang.com)
  • What is the abbreviation for p300/ CREB-binding protein (CBP)-interacting protein? (acronymsandslang.com)
  • What is shorthand of p300/ CREB-binding protein (CBP)-interacting protein? (acronymsandslang.com)
  • In this study, we found that thapsigargin-induced ER stress augmented recruitment of IFN regulatory factor-3, CREB binding protein/p300, and transcriptional machinery to the murine ifnb1 promoter during LPS stimulation. (jimmunol.org)
  • This grouping, termed the "enhanceosome," recruits histone acetylases, such as CREB binding protein (CBP/p300), a large flexible transcription coactivator that may interact simultaneously with multiple transcription factors (activating transcription factor [ATF]-2, c-Jun, p65, and IRF-3/7) ( 10 , 11 ). (jimmunol.org)
  • The Crebbp gene encodes the CREB binding protein and the Ep300 gene encodes the E1A binding protein p300, both of which are highly conserved coactivators that promote transcription and are important to the function of many hematopoietic transcription factors. (jax.org)
  • 2002. A transcription-factor-binding surface of coactivator p300 is required for haematopoiesis. (jax.org)
  • Here we report identification of the first three in vivo non-histone protein substrates of lysine propionylation in eukaryotic cells: p53, p300, and CREB-binding protein. (mcponline.org)
  • We also identified the first two in vivo eukaryotic lysine propionyltransferases, p300 and CREB-binding protein, and the first eukaryotic depropionylase, Sirt1. (mcponline.org)
  • Here, we report three three-dimensional solution structures of the bromodomains of the human transcriptional coactivators CREB-binding protein (CBP) and p300/CBP-associated factor (PCAF) bound to peptides derived from histone acetylation sites at lysines 36 and 9 in H3, and lysine 20 in H4. (proteopedia.org)
  • CREB-binding protein, also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene. (wikipedia.org)
  • CREBBP (CREB Binding Protein) is a Protein Coding gene. (genecards.org)
  • Description: A sandwich quantitative ELISA assay kit for detection of Human CREB Binding Protein (CREBBP) in samples from tissue homogenates or other biological fluids. (ialltjournal.org)
  • Description: A sandwich ELISA for quantitative measurement of Rat CREB binding protein(CREBBP) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (ialltjournal.org)
  • The CREBBP gene provides instructions for making CREB binding protein, which regulates the activity of many genes in tissues throughout the body. (medlineplus.gov)
  • Because one copy of the CREBBP gene is altered in people with Rubinstein-Taybi syndrome, their cells make only half of the normal amount of functional CREB binding protein. (medlineplus.gov)
  • Although researchers are uncertain how a reduction in the amount of this protein leads to the features of Rubinstein-Taybi syndrome, it is clear that variations in the CREBBP gene disrupt normal development before and after birth. (medlineplus.gov)
  • Mutations in the CREBBP (CREB-binding protein gene) cause Rubinstein-Taybi syndrome (RSTS). (cdc.gov)
  • Crebbp (CREB binding protein, also known as CBP) is a protein acetyltransferase that is required for normal development and acts as a tumor suppressor. (jax.org)
  • The family members share a 300-aa Rel homology region that is responsible for protein dimerization, nuclear localization, and DNA binding to κB elements in the enhancer regions of target genes. (pnas.org)
  • Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. (rcsb.org)
  • Functionally important binding sites for GATA-1 are found in the regulatory regions of essentially all erythroid-cell-specific genes ( 30 ). (asm.org)
  • CREB is a bZIP transcription factor that activates target genes through cAMP response elements. (biomol.com)
  • CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. (biomol.com)
  • Multiple functions of CREB-binding protein during postembryonic development: identification of target genes. (biomedcentral.com)
  • Knockdown of CBP caused a decrease in the expression of 1306 genes coding for transcription factors and other proteins associated with growth and development. (uky.edu)
  • One of the best characterized stimulus-induced transcription factors, cyclic AMP response element (CRE)-binding protein (CREB), activates transcription of target genes in response to a diverse array of stimuli, including peptide hormones, growth factors, and neuronal activity, that activate a variety of protein kinases including protein kinase A (PKA), pp90 ribosomal S6 kinase (pp90RSK), and Ca2+/calmodulin-dependent protein kinases (CaMKs)[corrected]. (nih.gov)
  • cAMP can stimulate the transcription of many activity-dependent genes via activation of the transcription factor, cAMP response element-binding protein (CREB). (jneurosci.org)
  • Sterol regulatory element-binding proteins (SREBPs) activate promoters for key genes of metabolism to keep pace with the cellular demand for lipids. (elsevier.com)
  • CONCLUSIONS Impaired expression of ICER contributes to elevation in CREB target genes and, therefore, to the development of insulin resistance in obesity. (diabetesjournals.org)
  • The transcription factor BTB and CNC homology 1 (Bach1) is widely expressed in most mammalian tissues and functions primarily as a transcriptional suppressor by heterodimerizing with small Maf proteins and binding to Maf recognition elements in the promoters of targeted genes. (hindawi.com)
  • Once formed, the Bach1-Maf heterodimers inhibit the transcription of many oxidative stress-response genes, including heme oxygenase-1 (HO-1) [ 2 ] and NADPH quinone oxidoreductase 1(NQO1) [ 3 ], by binding to Maf recognition elements (MAREs) in the gene promoters. (hindawi.com)
  • Bach1 competes with nuclear factor (erythroid-derived 2)-like-2 (Nrf2) for binding to the MAREs in oxidative stress-response genes. (hindawi.com)
  • HNF-1α regulates the expression of many liver-specific genes by directly binding to the promoter and enhancer regions, including albumin, fibrinogen, and α 1 -anti-trypsin genes (consensus site: GTTAATNATTAAC, where N = A, C, G, T, or no nucleotide) ( 8 , 9 ). (diabetesjournals.org)
  • Two genes with mutant versions that act as dominant suppressors of the wing phenotype were identified: the PKA-C1/DCO gene, encoding the catalytic subunit of cyclic AMP protein kinase, and ash1, a member of the trithorax group (trxG) of chromatin modifiers. (elsevier.com)
  • The serum response factor is a member of the MADS-box ( M CM1, A gamous, D eficiens, and S RF) box superfamily of transcription factors, [5] binding to the serum response element (SRE) in the promoter region of target genes. (wikiversity.org)
  • The murine double minute ( mdm2 ) oncogene , which codes for the Mdm2 protein, was originally cloned, along with two other genes (mdm1 and mdm3) from the transformed mouse cell line 3T3-DM. (wikipedia.org)
  • Simultaneously, a number of other DNA binding transcription factors are expressed and/or activated under redox control, such as Nuclear Respiratory Factor-1 (NRF-1), and lead to the induction of genes involved in both intracellular and mitochondria-specific repair mechanisms. (frontiersin.org)
  • RNA pol II transcribes all protein coding, snoRNA and snRNA genes d. (brainscape.com)
  • This causes a β-catenin cytoplasmic accumulation allowing entry into nucleus, where it exerts its action by binding to a clump of molecules and transcription factors, allowing to express the target genes, including the incretin hormones. (isciii.es)
  • Esto produce una acumulación de esta proteína en el citoplasma celular y permite su entrada al núcleo, donde ejerce su acción al unirse a una serie de moléculas y factores de transcripción, permitiendo de este modo que se expresen los genes diana, entre los que se encuentran los de las hormonas incretinas. (isciii.es)
  • Genomics is the study of all the genes in an organism, how genes and proteins work together and interact. (brainscape.com)
  • CREB-binding protein (CBP) is a coactivator that interacts with the cAMP-response element binding protein (CREB), a process dependent on the cAMP-dependent protein kinase A and its phosphorylation of CREB ( 21 , 22 ). (pnas.org)
  • Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. This modification stimulates interaction with one or more of the general transcription factors or, alternatively, allows recruitment of a co-activator. (nih.gov)
  • CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling. (biomol.com)
  • These results are consistent with a model in which CBP is constitutively bound to the SRE in a higher order complex that would facilitate the rapid transcriptional activation of c-fos by signaling-driven phosphorylation. (cnrs.fr)
  • These kinases all phosphorylate CREB at a particular residue, serine 133 (Ser133), and phosphorylation of Ser133 is required for CREB-mediated transcription. (nih.gov)
  • This review discusses the molecular mechanisms by which Ser133-phosphorylated CREB activates transcription, intracellular signaling pathways that lead to phosphorylation of CREB at Ser133, and features of each signaling pathway that impart specificity at the level of CREB activation. (nih.gov)
  • cAMP activates cAMP-dependent protein kinase (PKA), which can directly modulate effector proteins by phosphorylation at PKA-specific serine or threonine residues or can alter the levels of the effector proteins themselves by modulating gene expression. (jneurosci.org)
  • Although CREB phosphorylation is necessary, it may not be sufficient to initiate gene transcription. (jneurosci.org)
  • Regulation of genotoxic stress response by homeodomain-interacting protein kinase 2 through phosphorylation of cyclic AMP response element-binding protein at serine 271. (edu.sa)
  • Both classes show an increase in binding after phosphorylation of CREB by protein kinase A (PKA). (uni-muenchen.de)
  • An in vivo phosphorylation-dependent change in binding of CREB increases the occupancy of weak binding sites used for transactivation, such as the TATCRE, while high affinity sites may have constitutive binding of transcriptionally active and inactive CREB dimers, as demonstrated by in vivo footprinting at the PEPCK CRE. (uni-muenchen.de)
  • Phosphorylation of serine 311 by protein kinase C zeta type (PKCζ) serves the same purpose. (wikipedia.org)
  • The fact that RELA can be modified by a collection of kinases via phosphorylation at different sites/regions within the protein under different stimulations might suggest a synergistic effect of these modifications. (wikipedia.org)
  • Phosphorylation at these sites enhances NF-κB transcriptional response via tightened binding to transcription coactivators. (wikipedia.org)
  • In vivo studies revealed that RELA is also under acetylation modification in the nucleus, which is just as important as phosphorylation as a post-translational modification of proteins. (wikipedia.org)
  • Another signaling pathway activated by growth factor receptors involves the phosphorylation of signal transducers and activators of transcription (STAT). The duration and intensity of these signals are regulated by phosphatases and proteins that induce the activation of the JAK/STAT pathway, named suppressors of cytokine signaling (SOCS), and particularly SOCS1, a negative regulator of interleukin-6 (IL-6) and interferon (IFN-γ) signaling 8 . (nature.com)
  • Mapping the interaction of corepressor proteins (HDAC1, silencing mediator of retinoid and thyroid receptor/nuclear corepressor of retinoid receptor, and Sin3a) and HATs to IRF5 revealed distinct differences, including the dependence of IRF5 phosphorylation on HAT association resulting in IRF5 acetylation. (jimmunol.org)
  • Hippocampal cAMP levels were examined by enzyme-linked immunosorbent assay, whereas PKAca, phosphorylation of CREB (pCREB), CREB, and GLT1 in the hippocampus of rats were subjected to Western blot analysis. (dovepress.com)
  • Inhibition of PKA activity reduced cAMP response element-binding protein (CREB) phosphorylation, thereby inhibiting GH and PRL transcription and secretion. (sigmaaldrich.com)
  • Recent results suggest that novel CBP-mediated post-translational N-glycosylation activity alters the conformation of CBP-interacting proteins, leading to regulation of gene expression, cell growth and differentiation, Homeodomain interacting protein kinase 2 (HIPK2) phosphorylates several regions of CBP close to the N-terminal and close to the C-terminal region as well. (wikipedia.org)
  • Here we report that CREB phosphorylated by protein kinase A binds specifically to a nuclear protein of M(r) 265K which we term CBP (for CREB-binding protein). (nih.gov)
  • Fusion of a heterologous DNA-binding domain to the amino terminus of CBP enables the chimaeric protein to function as a protein kinase A-regulated transcriptional activator. (nih.gov)
  • Exposure of monocytes and macrophages to LPS results in activation of the mitogen-activated protein kinase (MAPK) pathway, including the extracellular signal-related kinase (ERK), c-jun NH 2 -terminal kinase (JNK), and p38 cascades (reviewed in reference 12 ). (asm.org)
  • Extracellular stimuli elicit changes in gene expression in target cells by activating intracellular protein kinase cascades that phosphorylate transcription factors within the nucleus. (nih.gov)
  • Kovács KA, Steinmann M, Halfon O, Magistretti PJ, Cardinaux J-R (2015) Complex regulation of CREB-binding protein by homeodomain-interacting protein kinase 2. (edu.sa)
  • Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2. (edu.sa)
  • Brain-derived neurotrophic factor (BDNF) and its receptor tropomyosin receptor kinases (TrkB), as well as extracellular signal-regulated kinase (ERK) and its downstream target c-AMP-responsive element binding protein (CREB) are strongly associated with the transmission of nociceptive information. (biologists.org)
  • Triggered by lipopolysaccharide (LPS), protein kinase A (PKA) specifically phosphorylates serine 276 in the RHD domain in the cytoplasm, controlling NF-κB DNA-binding and oligomerization. (wikipedia.org)
  • We found that the p90 ribosomal S6 kinase 2 (RSK2)-cAMP response element-binding protein (CREB) pathway is commonly activated in diverse metastatic human cancer cells, leading to up-regulation of a CREB transcription target Fascin-1. (novartis.com)
  • for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). (wikiversity.org)
  • Next, we also investigated the alterations of cyclic adenosine monophosphate (cAMP), protein kinase A (PKA) catalytic subunit (PKAca), cAMP response element-binding (CREB), and glutamate transporter 1 (GLT1) levels produced by GEBR-7b in the rats model of depression. (dovepress.com)
  • The results showed that BHBA decreased intracellular cAMP levels and a subsequent reduction in protein kinase A (PKA) activity. (sigmaaldrich.com)
  • Signaling through the cyclic adenosine monophosphate-dependent protein kinase [protein kinase A (PKA)] is an important and widely studied area of signal transduction research. (sciencemag.org)
  • cAMP has three direct intracellular targets: protein kinase A (PKA), the exchange protein activated by cAMP (Epac), and cyclic nucleotide-gated ion channels (CNGCs). (sciencemag.org)
  • The CREB protein domains, KIX, TAZ1 and TAZ2, each bind tightly to a sequence spanning both transactivation domains 9aaTADs of transcription factor p53. (wikipedia.org)
  • First isolated as a nuclear protein [Ref] that binds to cAMP-response element-binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. (wikipedia.org)
  • Cyclic AMP response element-binding protein (CREB) is a widely expressed transcription factor whose role in neuronal protection is now well established. (semanticscholar.org)
  • Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and transcription factor binding . (genecards.org)
  • Some of these putative co-regulatory proteins are members of transcription factor families that all bind to the same DNA sequence elements in vitro and are often expressed in the same cells. (elsevier.com)
  • CREB is a transcription factor implicated in the pathogenesis of multiple cancers. (elsevier.com)
  • Transcription factor p65 also known as nuclear factor NF-kappa-B p65 subunit is a protein that in humans is encoded by the RELA gene. (wikipedia.org)
  • Although full synergistic IFN-β production requires X-box binding protein 1 (XBP-1), this UPR-regulated transcription factor did not appreciably bind the ifnb1 promoter. (jimmunol.org)
  • The serum response factor (SRF) is a transcription factor protein that binds to the c-fos serum response element (SRE). (wikiversity.org)
  • Transcriptional responses to increased cAMP occur through activation of the cAMP response element-binding protein (CREB), cAMP response element modulator (CREM), and activating transcription factor 1 (ATF1) ( 9 ). (sciencemag.org)
  • Transactivation by both components of this complex, serum response factor (SRF) and the ternary complex factor Elk-1, can be potentiated by the coactivator CREB-binding protein (CBP). (cnrs.fr)
  • 2011) A small molecule binding to the coactivator CREB-binding protein blocks apoptosis in cardiomyocytes. (guidetopharmacology.org)
  • This protein shares regions of very high sequence similarity with protein EP300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. (wikipedia.org)
  • In diffuse large B-cell lymphoma (DLBCL), inactivating mutations of the histone acetyltransferases CREB-binding protein (CBP) and EP300 are common. (lu.se)
  • Our results suggest that depletion of CBP and EP300 levels leads to a strong reduction of CD20 expression, accompanied by reduced binding of PU.1 to the CD20 promoter. (lu.se)
  • In CBP-depleted, but not EP300-depleted cells, increased binding of FOXO1 to the CD20 promoter was observed. (lu.se)
  • This protein has also been shown to possess acetyltransferase activity, further implicating it in the accessibility of chromatin/histones for active transcription ( 32 , 33 ). (pnas.org)
  • The CREB binding protein ensures the DNA is ready for transcription by attaching a small molecule called an acetyl group to proteins called histones (a process called acetylation). (medlineplus.gov)
  • Histones are structural proteins that bind DNA and give chromosomes their shape. (medlineplus.gov)
  • The CREB-binding protein (CBP) pathway plays an important role in transcription and activity of acetyltransferase that acetylates lysine residues of histones and nonhistone proteins. (cdc.gov)
  • Besides histones, HATs and HDACs may also act on nonhistone proteins such as transcription factors. (jimmunol.org)
  • Lysine propionylation and butyrylation are protein modifications that were recently identified in histones. (mcponline.org)
  • BACKGROUND: Post-translational modifications (PTMs) of histones and other proteins are perturbed in tumours. (biomedsearch.com)
  • Recognition of acetyl-lysine by bromodomain proteins is not limited to histones. (cellsignal.com)
  • acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). (rcsb.org)
  • Acetylation in vitro did not alter the ability of GATA-1 to bind DNA, and mutations in either motif did not affect DNA binding of GATA-1 expressed in mammalian cells. (asm.org)
  • We found that acetylation of E2F1 is, instead, required to stabilize the protein in response to doxorubicin. (elsevier.com)
  • We suggest that MOZ may represent a chromatin-associated acetyltransferase, and raise the possibility that a dominant MOZ-CBP fusion protein could mediate leukaemogenesis via aberrant chromatin acetylation. (elsevier.com)
  • For instance, lysine 221 acetylation facilitates RELA dissociation from IκBα and enhances its DNA-binding affinity. (wikipedia.org)
  • Hung HL, Kim AY, Hong W, Rakowski C, Blobel GA. Stimulation of NF-E2 DNA binding by CREB-binding protein (CBP)-mediated acetylation. (proteopedia.org)
  • After export into the cytoplasm, Bach1 forms fiber-like structures on microtubules by colocalizing with intracellular hyaluronic acid-binding protein (IHABP), which regulates the subcellular localization of Bach1 [ 13 ]. (hindawi.com)
  • The CREB protein carries out its function by activating transcription, where interaction with transcription factors is managed by one or more CREB domains: the nuclear receptor interaction domain (RID), the KIX domain (CREB and MYB interaction domain), the cysteine/histidine regions (TAZ1/CH1 and TAZ2/CH3) and the interferon response binding domain (IBiD). (wikipedia.org)
  • The ability of Tat to interact physically and functionally with this coactivator provides a mechanism to assemble a basal transcription complex which may subsequently respond to the effect of Tat on transcriptional elongation and represents a novel interaction between an RNA binding protein and a transcriptional coactivator. (asm.org)
  • Recruitment of human cyclin T1 to nuclear bodies through direct interaction with the PML protein. (semanticscholar.org)
  • To further explore the role of MIER1 in chromatin remodeling, we investigated the functional interaction of MIER1 with the histone acetyltransferase (HAT), Creb-binding protein (CBP). (biomedcentral.com)
  • The small-molecule ICG-001 binds cAMP-responsive element binding (CREB)-binding protein (CBP) to disrupt its interaction with β-catenin and inhibit CBP function as a coactivator of Wnt/β-catenin-mediated transcription. (qxmd.com)
  • The N-terminal region of Bach1 contains a BTB/POZ domain, which functions as a protein interaction motif, while the C-terminal bZip domain binds to DNA [ 1 ] and mediates the heterodimerization of Bach1 with small Maf proteins (e.g. (hindawi.com)
  • RHD is involved in DNA binding, dimerization and NF-κB/REL inhibitor interaction. (wikipedia.org)
  • In this CBP KIX mutant strain the interaction between the phosphorylated CREB and CREB-binding protein is blocked. (jax.org)
  • The interaction between the phosphorylated CREB and CREB-binding protein is blocked in these mutants. (jax.org)
  • [10] Interaction of SRF with other proteins, such as steroid hormone receptors, may contribute to regulation of muscle growth by steroids. (wikiversity.org)
  • Using either endogenous p53 or a second fluorescently tagged fusion of p53 with the rotavirus NSP5 protein, we demonstrated p53 oligomerization as well as p53 association with another of its cellular interaction partners, the CREB-binding proteins, within the inclusions. (mcponline.org)
  • Numerous methods are available for addressing whether proteins associate either directly ("interaction") or indirectly (through bridging molecules), but many of these methods can be technically demanding, can be limited by the strength of the association, or cannot resolve whether the association indeed occurs inside cells. (mcponline.org)
  • Nuclear factor κB binding sites were shown to be primarily responsible for the positive activity contributed by the HS1,2 and HS4 regions, and we observed the in vivo interaction of these factors with the human immunoglobulin heavy chain gene enhancer regions in t(14;18) cells. (aacrjournals.org)
  • Unexpectedly, we discovered a small molecule 11 (653-47) that can potentiate the CREB inhibitory activity of 666-15 although 653-47 alone does not inhibit CREB. (elsevier.com)
  • In LPS-stimulated macrophages, the ERK substrates Ets and Elk-1 bind to the TNF-α promoter in vivo. (asm.org)
  • The transcription factors ATF-2, c-jun, Egr-1, and Sp1 are also inducibly recruited to the TNF-α promoter in vivo, and the binding sites for each of these activators are required for LPS-stimulated TNF-α gene expression. (asm.org)
  • The finding that a distinct set of transcription factors associates with a fixed set of binding sites on the TNF-α promoter in response to LPS stimulation lends new insights into the mechanisms by which complex patterns of gene regulation are achieved. (asm.org)
  • We identify TNF-α promoter elements critical for LPS induction of the gene and demonstrate that two Sp1 binding sites and three Ets binding sites, in addition to a cyclic AMP response element (CRE)-like site and an Egr site, are critical for LPS induction of the TNF-α gene. (asm.org)
  • Consistent with this functional analysis of the TNF-α promoter, using chromatin immunoprecipitation and formaldehyde crosslinking (ChIP) assays, we directly detect LPS-inducible binding of the transcription factors ATF-2, c-jun, Ets-1 and -2, Elk-1, Egr-1, and Sp1 to the endogenous TNF-α promoter. (asm.org)
  • Remarkably, a set of TNF-α promoter elements, which bind NFAT upon induction of the gene by calcineurin-dependent stimuli, also bind the ERK-targeted Ets and Elk proteins and are required in LPS-stimulated TNF-α gene expression. (asm.org)
  • In cells infected with HIV type 1 (HIV-1), the integrated viral promoter is present in a chromatin-bound conformation and is transcriptionally silent in the absence of stimulation. (semanticscholar.org)
  • However, inhibition is not dependent upon the presence of a CRE in the promoter, and does not involve heterodimer formation between CREB and the activator. (umassmed.edu)
  • In each SREBP-regulated promoter, at least one ubiquitous co-regulatory factor that binds to a neighboring recognition site is also required for efficient gene induction. (elsevier.com)
  • We have used the chromatin immunoprecipitation (ChIP) technique coupled with a transient complementation assay in Drosophila SL2 cells to directly compare the ability of two members of the CREB/ATF family to function as co-regulatory proteins for SREBP-dependent activation of the HMG-CoA reductase promoter. (elsevier.com)
  • Binding of the transcription factors PU.1 and FOXO1 to the CD20 promoter was determined by chromatin immunoprecipitation coupled with quantitative polymerase chain reaction. (lu.se)
  • The mouse CREB (cAMP responsive element binding protein) gene: structure, promoter analysis, and chromosomal localization," Co-Author, (1992) Genomics 13 (4), 974-982. (taylorenglish.com)
  • We report that CCAAT/enhancer-binding protein (C/EBP)-β interacts with ARE/EpRE in the rGST-Ya promoter and that aryl hydrocarbon receptor (AhR) is present within the protein complex binding to the C/EBP site. (elsevier.com)
  • Trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. (uniprot.org)
  • Binds also to the transferrin receptor promoter. (uniprot.org)
  • The nuclear localization signal of NF-κB is masked by the binding of an inhibitory protein, IκB, sequestering NF-κB in the cytoplasm in unstimulated cells. (pnas.org)
  • As these data are limited to the dynamically distributed PML protein, we chose to focus on providing a role for the PML-containing nuclear body itself. (pnas.org)
  • Immunohistochemical analysis demonstrates the PML-containing nuclear body to be composed of at least four proteins, including PML, PML-associated factor (PAF31) (J. D. Chen and R.M.E., unpublished data), the SP-100 autoantigen of primary biliary cirrhosis, and the nuclear matrix protein NDP55 ( 20 - 23 ). (pnas.org)
  • A potential solution to this paradigm is to have a ubiquitously expressed protein act as a conduit for linking signaling pathways to nuclear transcription factors by interacting with (1) terminal members of the many signaling cascades and (2) the specific combination of transcription factors that are expressed in each different cell type. (genetics.org)
  • Similar polyacidic tracks are found in several nuclear proteins whose functions are linked to chromatin decondensation, nucleosome assembly/disassembly and establishment of chromatin structures competent for transcription ( Earnshaw, 1987 ). (embopress.org)
  • Strikingly, Ets and Elk-1 bind to two TNF-α nuclear factor of activated T cells (NFAT)-binding sites, which are required for calcineurin and NFAT-dependent TNF-α gene expression in lymphocytes. (asm.org)
  • Calcineurin targets the nuclear factor of activated T cells (NFAT) family of proteins (reviewed in references 11 and 38 ), which are critical for TNF-α gene expression by calcineurin-dependent signal transduction pathways ( 15 , 48 , 49 ). (asm.org)
  • RESEARCH DESIGN AND METHODS Total RNA and nuclear proteins were prepared from visceral adipose tissue (VAT) of human nonobese or obese subjects and white adipose tissue (WAT) of C57Bl6-Rj mice that were fed with normal or high-fat diet for 16 weeks. (diabetesjournals.org)
  • Transient expression of the constructs in COS-1 (Fig. 3C) or HEK293 cells (not really proven) revealed that while every one of the truncated CBP protein had been nuclear just full-length CBP demonstrated a solid association with nuclear physiques. (movd2016.org)
  • Under normal physiological conditions, nuclear Nrf2 contributes to vascular protection by inducing expression of the glutamate cysteine ligase modulatory subunit (GCLM) and the light chain component of system x c − (xCT) in human endothelial cells, while cytoplasmic Nrf2 is bound and inhibited by Kelch-like ECH-associated protein 1 (Keap1) [ 15 ]. (hindawi.com)
  • RELA, also known as p65, is a REL-associated protein involved in NF-κB heterodimer formation, nuclear translocation and activation. (wikipedia.org)
  • Protein stability and expression are unaltered as detected by Western Blot analysis of nuclear extracts from brain tissue of homozygous animals. (jax.org)
  • Androgen deprivation strongly inhibits the ligand-dependent nuclear accumulation of pathogenic AR protein, resulting in a striking improvement in neurological and histopathological findings of male mice. (jneurosci.org)
  • Inhibition requires that the CREB dimerization and DNA-binding domains are intact. (umassmed.edu)
  • The p65 subunit, similar to two others in the κB family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein ( 8 ). (pnas.org)
  • In addition, PML and the transactivation cofactor, CREB binding protein (CBP), colocalize within the nucleus. (pnas.org)
  • Importantly, the CBP-binding activity of Nrf2 deletion mutants positively correlated with their transactivation activity. (nih.gov)
  • Our results indicate that Nrf2 exploits the cooperative binding of two independent transactivation domains to CBP in the acquisition of a potent transactivation activity. (nih.gov)
  • DNA binding and glutathione S-transferase pull-down assays demonstrate that binding requires Elk-1(1-212) but not the C-terminal transactivation domain. (cnrs.fr)
  • Like other proteins in this complex, RELA contains a N-terminal REL-homology domain (RHD), and also a C-terminal transactivation domain (TAD). (wikipedia.org)
  • The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. (wikipedia.org)
  • At the chromosome 8 breakpoint we identify a novel gene, MOZ, which encodes a 2,004-amino-acid protein characterized by two C4HC3 zinc fingers and a single C2HC zinc finger in conjunction with a putative acetyltransferase signature. (elsevier.com)
  • On the basis of this function, CREB binding protein is called a histone acetyltransferase. (medlineplus.gov)
  • The lysine acetyltransferase CREB binding protein (CBP) is required for chromatin changes and transcription at many gene promoters. (movd2016.org)
  • This strain may be useful in studies of the role of the CREB-binding protein histone acetyltransferase and hematopoiesis. (jax.org)
  • HBO1, histone acetyltransferase bound to ORC1. (mcponline.org)
  • Mitochondrial cyclic AMP response element-binding protein (CREB) mediates mitochondrial gene expression and neuronal survival. (semanticscholar.org)
  • Cyclic AMP response element binding protein (CREB) activates transcription of cAMP response element (CRE)-containing promoters following an elevation of intracellular cAMP. (umassmed.edu)
  • Cyclic AMP treatment of hepatoma cells leads to increased protein binding at the cyclic AMP response element (CRE) of the tyrosine aminotransferase (TAT) gene in vivo, as revealed by genomic footprinting, whereas no increase is observed at the CRE of the phosphoenolpyruvate carboxykinase (PEPCK) gene. (uni-muenchen.de)
  • Here, we have examined the effects of CsA on the DNA-binding activity of the cyclic AMP response element-binding protein (CREB) and cell viability, and the effects of CREB on cathepsin B and L synthesis and activity in HGFs. (elsevier.com)
  • Collectively, these proteins interact with components of the basal transcriptional apparatus to effect dramatic changes in gene expression (for review, see ref. 1 ). (pnas.org)
  • A second class of proteins important in the initiation of transcription by RNA polymerase II are the coactivators (i.e., proteins that bridge the transcriptional activators and the components of the basal transcriptional apparatus). (pnas.org)
  • The HIV-1 Tat protein binds to a stem-loop structure at the 5' end of viral mRNA and relieves this inhibition by inducing a remodeling of the nucleosome arrangement downstream of the transcription-initiation site. (semanticscholar.org)
  • Histone 4 peptide binding assays demonstrate that this inhibition of HAT activity is not the result of interference with histone binding. (biomedcentral.com)
  • Inhibition of Wnt/beta-catenin/CREB binding protein (CBP) signaling reverses pulmonary fibrosis. (qxmd.com)
  • β-Hydroxybutyric sodium salt inhibition of growth hormone and prolactin secretion via the cAMP/PKA/CREB and AMPK signaling pathways in dairy cow anterior pituitary cells. (sigmaaldrich.com)
  • HER-2/neu overexpression resulted in the induction and activation of CREB protein in vitro and in vivo , whereas short hairpin RNA (shRNA)-mediated inhibition of HER-2/neu correlated with downregulated CREB activity. (aacrjournals.org)
  • CREB activation in HER-2/neu-transformed cells enhanced distinct signal transduction pathways, whereas their inhibition negatively interfered with CREB expression and/or activation. (aacrjournals.org)
  • Solution NMR structure of ischemin bound to the bromodomain of CBP. (guidetopharmacology.org)
  • Identified in over one hundred proteins from yeast to man, the approximately 110 amino acid bromodomain can bind to acetylated lysine residues. (cellsignal.com)
  • For example, the bromodomain of CREB binding protein transcriptional coactivator (CBP) allows for recognition of p53 with acetylated Lys382. (cellsignal.com)
  • From structural and biochemical binding analyses, we determine consensus histone recognition by the bromodomains of PCAF and CBP, which represent two different subgroups of the bromodomain family. (proteopedia.org)
  • A heterodimer composed of a p50 subunit bound to a p65 (Rel A) subunit is the common dimer. (pnas.org)
  • Polyglutamine-induced transcriptional dysregulation of the dynactin p150 subunit (dynactin 1), an axonal motor-associated protein, resulted in perturbation of retrograde axonal transport in spinal motor neurons in the early stage of the disease. (jneurosci.org)
  • Agonist occupation of G s -coupled receptors catalyzes the exchange of guanosine diphosphate (GDP) to guanosine triphosphate (GTP) on the α subunit of the G protein, causing a conformational change and dissociation of this complex from the βγ subunits. (sciencemag.org)
  • The KIX domain mediates binding to HIV-1 Tat. (rcsb.org)
  • Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME. (childrensmercy.org)
  • Comprehensive screening of CREB-binding protein gene mutations among patients with Rubinstein-Taybi syndrome using denaturing high-performance liqu. (cdc.gov)
  • It interacts with a significant percentage of mammalian transcriptional regulatory proteins and mutations in the gene have been found in hematopoietic and epithelial tumors. (jax.org)
  • These CBP KIX mice carry triple point mutations (Tyr650Ala, Ala654Gln, and Tyr658Ala ) in the KIX domain encoding exon, resulting in the production of a hypomorphic protein that cannot bind CREB or c-Myb via this domain. (jax.org)
  • Novel genetic variants in HDAC2 and PPARGC1A of the CREB-binding protein pathway predict survival of non-small-cell lung cancer. (cdc.gov)
  • Accumulating evidence suggests that O -GlcNAc transferase (OGT), an enzyme responsible for modification of proteins with N -acetylglucosamine, may act as a nutrient sensor that links hexosamine biosynthesis pathway to oncogenic signaling and regulation of factors involved in glucose and lipid metabolism. (frontiersin.org)
  • There is also evidence that glucose, through the hexosamine pathway, can induces autocrine activation of Wnt signalling pathway by stimulating secretion of Wnt proteins. (isciii.es)
  • In this study, we investigated whether impaired expression of the inducible cAMP early repressor (ICER), a transcriptional antagonist of CREB, is responsible for the increased CREB activity in adipocytes of obese mice and humans. (diabetesjournals.org)
  • The impact of increased Creb activity has been further validated by overexpressing a dominant-negative form of Creb in adipocytes ( 9 ). (diabetesjournals.org)
  • ProTα binds to CBP in vivo and in vitro . (embopress.org)
  • This model was confirmed by the findings that the proteins interact strongly in vitro and colocalize at specific sites on polytene chromosomes. (elsevier.com)
  • This ELISA kit applies to the in vitro quantitative determination of Rat CREB concentrations in serum, plasma and other biological fluids. (srgroupchemical.com)
  • To establish a mechanistic link between HER-2/neu-mediated transformation and CREB protein expression and function, in vitro models of HER-2/neu-overexpressing and HER-2/neu-negative/silenced counterparts as well as human mammary carcinoma lesions with defined HER-2/neu status were used. (aacrjournals.org)
  • A stimulus-specific role for CREB-binding protein (CBP) in T cell receptor-activated tumor necrosis factor alpha gene expression. (duke.edu)
  • The present work aimed at analyzing the SOCS1 cell signaling and expression of proteins relevant to tumor development. (nature.com)
  • We also observed that the protein expression patterns of RSK2 and Fascin-1 correlate in primary human tumor tissue samples from head and neck squamous cell carcinoma patients. (novartis.com)
  • Mdm2 protein functions both as an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and as an inhibitor of p53 transcriptional activation. (wikipedia.org)
  • The E3 ubiquitin ligase MDM2 is a negative regulator of the p53 tumor suppressor protein. (wikipedia.org)
  • Studies have also shown other intracellular signal transduction pathways culminating in the activation of CREB, as well as various paradigms of drug abuse which activate this key molecular substrate. (scirp.org)
  • We have previously shown that the transcriptional cofactor CREB-binding protein (CBP) binds to the zinc finger domain of GATA-1, markedly stimulates the transcriptional activity of GATA-1, and is required for erythroid differentiation. (asm.org)
  • Our previous studies indicated that a widely expressed transcriptional cofactor, CREB-binding protein (CBP), is a potent coactivator of GATA-1 ( 4 ). (asm.org)
  • To date, the cellular cofactors that bind to Tat have not been implicated in chromatin remodeling. (asm.org)
  • The trxG proteins are thought to maintain gene expression during development by creating domains of open chromatin structure. (elsevier.com)
  • Our results thus implicate a second class of chromatin-associated proteins in mediating dCBP function and imply that dCBP might be involved in the regulation of higher-order chromatin structure. (elsevier.com)
  • Bromodomains are generally found in proteins that regulate chromatin structure and gene expression, such as histone acetyltransferases and the ATPase component of certain nucleosomes-remodeling complexes. (cellsignal.com)
  • In fact, the specific inhibitor of CBP, adenovirus E1A protein, significantly reduced Nrf2 activity. (nih.gov)
  • The cAMP response element binding protein, CREB, is a potent inhibitor" by Frederic P. Lemaigre, Christopher I. Ace et al. (umassmed.edu)
  • Previously, we identified 666-15 as a potent CREB inhibitor. (elsevier.com)
  • Xie, F, Fan, Q, Li, BX & Xiao, X 2019, ' Discovery of a Synergistic Inhibitor of cAMP-Response Element Binding Protein (CREB)-Mediated Gene Transcription with 666 - 15 ', Journal of Medicinal Chemistry . (elsevier.com)
  • We propose that CBP may participate in cAMP-regulated gene expression by interacting with the activated phosphorylated form of CREB. (nih.gov)
  • 2001). RNA-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. (rutgers.edu)
  • For PML-green fluorescent protein (GFP) expression, cells were microinjected with a CMX PML-GFP DNA expression construct at 50-200 ng/μl and incubated overnight to allow for expression. (pnas.org)
  • The expression patterns of specific DNA-binding factors that control eye development add an additional layer of complexity ( K umar and M oses 1997 ). (genetics.org)
  • This article examined the expression levels of the p53 protein and CBP as well as their diagnostic value in a biopsy sample. (koreamed.org)
  • In some cases, signaling pathways target additional sites on CREB or proteins associated with CREB, permitting CREB to regulate distinct programs of gene expression under different conditions of stimulation. (nih.gov)
  • Transcriptional activity of Creb is abnormally elevated in adipocytes of obese mice fed with a high-fat diet (HFD), resulting in elevation of the expression of its target gene, Atf3 ( 9 ). (diabetesjournals.org)
  • Results showed that BDNF, TrkB, phosphor(p)-ERK and p-CREB were up-regulated in the brain neurons of both male and female rats with NTG-induced migraine compared to non-migraine control, whereas their expression levels were decreased in headache-free intervals of the migraine compared to migraine attacks. (biologists.org)
  • Female ovariectomized rats showed significant reduction in the expression of BDNF, TrkB, p-CREB and p-ERK in both attacks and intervals of NTG-induced migraine, relative to rats that have their ovaries. (biologists.org)
  • This research investigated the effects of administration of high doses of DS seeds on the expression of CREB protein in both male and female rats' frontal cortices and its implication in addiction and neurodegeneration. (scirp.org)
  • The rats were euthanized and an analysis of variance (ANOVA) was computed to detect a significant main difference of DS effect on CREB expression for each group, while post hoc Bonferroni Test compared CREB protein expression between male and female groups. (scirp.org)
  • Result: There were significant differences in the expression of CREB protein between the sub-groups and between the male and female rats of treated sub-group (p (scirp.org)
  • Site-direct mutagenesis and footprint analyses showed that the HNF-1α binding site (+200 to +218) is critical in human GLUT2 gene expression. (diabetesjournals.org)
  • Expression of mutant CREB shifted the dose-learning curve for isoproterenol to the right such that a higher dose was required to induce learning. (mun.ca)
  • Expression of CREB shifted the dose-learning curve for isoproterenol to the left, with a lower dose now producing learning. (mun.ca)
  • Myocyte enhancer factor-2 (MEF2) proteins are a family of transcription factors which through control of gene expression are important regulators of cellular differentiation and consequently play a critical role in embryonic development. (wikiversity.org)
  • CREB downregulation in HER-2/neu-transformed cells by shRNA and by the inhibitors KG-501 and lapatinib caused morphologic changes, reduced cell proliferation with G 0 -G 1 cell-cycle arrest, which was rescued by CREB expression. (aacrjournals.org)
  • The literature contains reports of the promyelocytic leukemia gene product PML protein functioning as an enhancer of transcription ( 1 ) as well as a suppressor of transcription and growth ( 2 ). (pnas.org)
  • Chen, YH & Ramos, KS 2000, ' A CCAAT/enhancer-binding protein site within antioxidant/electrophile response element along with CREB-binding protein participate in the negative regulation of rat GST-Ya, gene in vascular smooth muscle cells ', Journal of Biological Chemistry , vol. 275, no. 35, pp. 27366-27376. (elsevier.com)
  • In addition, two Sp1 binding sites in HS4 were also found to positively influence bcl-2 activity, and Sp1 was observed to interact with the human HS4 enhancer in vivo . (aacrjournals.org)
  • Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2. (biomol.com)
  • they include actions on other protein kinases and signaling molecules and also on basic cellular functions, such as transcription. (sciencemag.org)
  • This is followed by the rapid translocation of NF-κB to the nucleus where it binds to specific κB elements ( 13 , 14 ). (pnas.org)
  • Prothymosin α (ProTα) is a histone H1‐binding protein localized in sites of active transcription in the nucleus. (embopress.org)
  • The IVT proteins had been useful for SUMO assays using recombinant after that … However deletion from the SUMOylated area did not influence the power of CBP to localise towards Ganetespib the nucleus (Figs. 2C and 4A) or its work Ganetespib as a coactivator. (movd2016.org)
  • It can translocate to the cell nucleus and bind to NF-κB p65. (nature.com)
  • The Ca 2+ /cAMP response element-binding protein (CREB) was initially identified as the main interlocutor in the dialogue between the synapse and the nucleus [1]. (wikiversity.org)
  • The NICD translocates to the nucleus, where it forms a complex with the DNA binding protein CSL, displacing a histone deacetylase (HDAc)-co-repressor (CoR) complex from CSL. (genome.jp)
  • Bromodomains (BRDs) are epigenetic reader domains that selectively recognize acetylated lysine residues on the tails of histone proteins, and are the only known protein modules that can target acetylated lysine residues. (genecards.org)
  • We used mass spectrometry to map lysine propionylation sites within these three proteins. (mcponline.org)
  • Several lysine residues in p53 C-terminus have been identified as the sites of ubiquitination, and it has been shown that p53 protein levels are downregulated by Mdm2 in a proteasome-dependent manner. (wikipedia.org)
  • Bromodomains of chromosomal proteins function as acetyl-lysine (Kac) binding domains. (proteopedia.org)
  • Transcriptional regulation, a critical control mechanism in fundamental biologic processes, requires the participation of several classes of proteins: those that bind specific DNA sequences, those that associate with transcriptional regulators through protein-protein interactions, known as transcriptional coactivators or corepressors, and those that perform an architectural function. (pnas.org)
  • CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. (biomol.com)
  • These results suggest that CREB is essential for the CsA-mediated down-regulation of cathepsin B and L synthesis in HGFs. (elsevier.com)
  • Taken together, our findings for the first time link RSK2-CREB signaling to filopodia formation and bundling through the up-regulation of Fascin-1, providing a proinvasive and prometastatic advantage to human cancers. (novartis.com)
  • Receptors coupled to a different G protein, G i , cause down-regulation of adenylyl cyclase activity and consequent lowering of cAMP concentrations ( Fig. 1 ). (sciencemag.org)
  • They result in changes to single protein building blocks (amino acids) in CREB binding protein. (medlineplus.gov)
  • RELA protein has four isoforms, the longest and the predominant one being 551 amino acids. (wikipedia.org)
  • The CREB-binding protein(CBP) is a transcriptional co-activators of various sequence-specific DNA-binding transcription factors and is involved in a wide variety of cellular activities, such as DNA repair, cell growth, differentiation, and apoptosis. (koreamed.org)
  • Background: cAMP response element-binding protein (CREB) is one of the cellular transcription factors found in neurons. (scirp.org)
  • Protein-protein associations are vital to cellular functions. (mcponline.org)
  • The cyclic (c)AMP responsive element binding protein (CREB) plays a key role in many cellular processes, including differentiation, proliferation, and signal transduction. (aacrjournals.org)
  • Epac is a guanine nucleotide exchange factor for the small G protein Rap1 and has been implicated in a number of cellular processes such as insulin secretion, neurotransmitter release, and integrin-mediated cell adhesion ( 4 - 6 ). (sciencemag.org)
  • As expected from this shift, CREB overexpression interfered with learning induced by stroking. (mun.ca)
  • They support evidence that CREB overexpression can be deleterious and suggest the hypothesis of an optimal pCREB window for learning. (mun.ca)
  • Overexpression of CREB-binding protein (CBP) nullified repression of rGST-Ya transcription. (elsevier.com)
  • Furthermore, CREB overexpression was found in tumors of distinct origin and evidence suggests an association with tumorigenicity. (aacrjournals.org)
  • We report here that ProTα physically interacts with the CREB‐binding protein (CBP), which is a versatile transcription co‐activator. (embopress.org)
  • First, CBP interacts with TFIIB ( 23 ), TATA-binding protein ( 1 , 12 , 37 , 40 ), and RNA polymerase II ( 10 , 21 , 27 ) and could thus serve as a bridging molecule between DNA-bound transcription factors and the basal transcription machinery. (asm.org)
  • Neh4 and Neh5 both individually and cooperatively bind to CBP (CREB (cAMP Responsive Element Binding protein) Binding Protein). (nih.gov)
  • It's possible small volumes of the Mouse cAMP response element binding protein(CREB)ELISA Kit to stick on the lids and on the walls of the vials due to handling and transportation. (antibody-antibodies.com)
  • However, in mouse cortical neuron cultures, prior to synaptogenesis, neither cAMP nor dopamine, which acts via cAMP, stimulated CREB-dependent gene transcription when NR2B-containing NMDA receptors (NMDARs) were blocked. (jneurosci.org)
  • Neuronal levels of cAMP are regulated by G-protein-coupled receptors linked to adenylyl cyclase (AC). (jneurosci.org)
  • Here, we report that, in immature neurons from embryonic mouse cortex, prior to synaptogenesis, DA and cAMP do not stimulate CREB-dependent gene transcription when NMDA receptors (NMDARs) are blocked. (jneurosci.org)
  • Our results suggest that cAMP stimulates CREB-dependent gene transcription via a two-step process by inducing release of an excitatory amino acid, identified here as l -aspartate, which then activates NMDARs, leading to Ca 2+ entry and gene transcription. (jneurosci.org)
  • Interactions between β-catenin and transforming growth factor-β signaling pathways mediate epithelial-mesenchymal transition and are dependent on the transcriptional co-activator cAMP-response element-binding protein (CREB)-binding protein (CBP). (qxmd.com)
  • OBJECTIVE Increase in adipose cAMP-responsive element\x{2013}binding protein (CREB) activity promotes adipocyte dysfunction and systemic insulin resistance in obese mice. (diabetesjournals.org)
  • This involves the cAMP-responsive element\x{2013}binding protein (CREB) transcriptional factors ( 9 ). (diabetesjournals.org)
  • The activity of the CREB factors is finely tuned by the level of the inducible cAMP early repressor (ICER). (diabetesjournals.org)
  • However, repeated GEBR-7b administration significantly reversed CUS-induced depression-like behavior and changes of cAMP/PKA/CREB/GLT1 signaling. (dovepress.com)
  • cAMP is synthesized from adenosine triphosphate (ATP) by adenylyl cyclase and is broken down to 5′ AMP by a class of proteins known as phosphodiesterases (PDEs) ( 1 , 2 ). (sciencemag.org)
  • The proteins immunoprecipitated by anti-CREB antibodies were analyzed by ESI-Q-TOF-MS in Bel-7402 cells for three independent times. (hindawi.com)
  • b) Endogenous CREB was immunoprecipitated by anti-CREB antibodies in Bel-7402 and SMMC-7721 cells, and the indicated proteins were measured by WB. (hindawi.com)
  • Cells were obtained for IF by anti-CREB and corresponding antibodies. (hindawi.com)
  • CREB-binding protein homolog - fruit. (imp.ac.at)
  • Mouse double minute 2 homolog ( MDM2 ) also known as E3 ubiquitin-protein ligase Mdm2 is a protein that in humans is encoded by the MDM2 gene . (wikipedia.org)
  • In both examples, we further localized a region of the recruited protein that is key to its recruitment. (mcponline.org)
  • These mechanisms are often mediated by histone linkers or by proteins associated with the recruitment of DNA-binding proteins, HDACI and II interacting proteins and transcriptional activators, coactivators or corepressors. (omicsonline.org)
  • Functional analysis, using HEK293 cells, shows that the CBP bound to MIER1 in vivo has no detectable HAT activity. (biomedcentral.com)
  • The molecular components involved in the two protein modification pathways are unknown, hindering further functional studies. (mcponline.org)
  • ILF2 was identified to directly interact with CREB. (hindawi.com)
  • Using immunocolocalization, we showed that the ASH1 protein is specifically expressed in the majority of the dCBP-overexpressing cells, suggesting that these proteins have the potential to interact biochemically. (elsevier.com)
  • This cell damage caused by oxidative and nitrosative stress leads to mitochondrial protein, DNA, and lipid modifications, which inhibits energy production and contractile function, potentially leading to cell necrosis and/or apoptosis. (frontiersin.org)
  • For instance, IKK2 is found to be mainly responsible for phosphorylating serine 536 in monocytes and macrophages, or in CD40 receptor binding in hepatic stellate cells. (wikipedia.org)
  • Further, CsA has no effect on cell viability and dramatically impairs CREB-DNA binding activity. (elsevier.com)
  • Importantly, the synthesis of cathepsin B and L is down-regulated, and their activity is also significantly impaired in HGFs transfected with plasmid expressing dominant-negative CREB. (elsevier.com)
  • A unique synaptic activity-responsive element (SARE) sequence, composed of the consensus binding sites for SRF, MEF2 and CREB, is necessary for control of transcriptional upregulation of the Arc gene in response to synaptic activity. (wikiversity.org)
  • In this study, we detected the effects of BHBA on the activities of G protein signaling pathways, AMPK-α activity, GH, and PRL gene transcription, and GH and PRL secretion in dairy cow anterior pituitary cells (DCAPCs). (sigmaaldrich.com)
  • Mdm2 has been identified as a p53 interacting protein that represses p53 transcriptional activity. (wikipedia.org)
  • Adenylyl cyclase activity can be modulated by agonist binding at GPCRs. (sciencemag.org)
  • Strikingly, CREB inhibits transcription of multiple activators, whose DNA-binding domains and activation regions are unrelated to one another. (umassmed.edu)
  • abstract = "CREB-binding protein (CBP) is a coactivator for multiple transcription factors that transduce a variety of signaling pathways. (elsevier.com)
  • Three of these proteins, including PML, T18 ( 14 ), and Ret ( 15 ), participate as fusion proteins that transform through unknown mechanisms. (pnas.org)
  • Purified GST (lane 1) or GST fusion proteins (2 μg each) encoding different CBP fragments (1-1098, 1098-1620, 1620-1897, 1897-2440, 1-771, lanes 2-6) were immobilized on glutathione-agarose beads and incubated with 2 μg of ProTα as indicated in Methods. (embopress.org)