Creatine Kinase
A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins.
Dysprosium
Isoenzymes
Creatine
Phosphatidylinositol 3-Kinases
Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.
Clinical Enzyme Tests
Phosphocreatine
MAP Kinase Signaling System
An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.
Protein Kinases
Protein-Serine-Threonine Kinases
Arginine Kinase
Calcium-Calmodulin-Dependent Protein Kinases
A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)
src-Family Kinases
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
Myocardium
L-Lactate Dehydrogenase
Protein Kinase C
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
Adenosine Triphosphate
Muscle, Skeletal
p38 Mitogen-Activated Protein Kinases
A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.
Electrophoresis, Cellulose Acetate
Cyclic AMP-Dependent Protein Kinases
Mitogen-Activated Protein Kinase 1
Pyruvate Kinase
Adenylate Kinase
Adenosine Diphosphate
p21-Activated Kinases
Mitogen-Activated Protein Kinase Kinases
A serine-threonine protein kinase family whose members are components in protein kinase cascades activated by diverse stimuli. These MAPK kinases phosphorylate MITOGEN-ACTIVATED PROTEIN KINASES and are themselves phosphorylated by MAP KINASE KINASE KINASES. JNK kinases (also known as SAPK kinases) are a subfamily.
JNK Mitogen-Activated Protein Kinases
Guanidinoacetate N-Methyltransferase
Mitogen-Activated Protein Kinase 3
Magnetic Resonance Spectroscopy
Rabbits
Amidinotransferases
Protein-Tyrosine Kinases
Myoglobin
Phosphorylation
CDC2 Protein Kinase
Phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the CELL CYCLE. It is the catalytic subunit of the MATURATION-PROMOTING FACTOR and complexes with both CYCLIN A and CYCLIN B in mammalian cells. The maximal activity of cyclin-dependent kinase 1 is achieved when it is fully dephosphorylated.
Cyclin-Dependent Kinases
MAP Kinase Kinase Kinases
Muscle Fibers, Slow-Twitch
Muscle Fibers, Fast-Twitch
Muscle Fibers, Skeletal
Large, multinucleate single cells, either cylindrical or prismatic in shape, that form the basic unit of SKELETAL MUSCLE. They consist of MYOFIBRILS enclosed within and attached to the SARCOLEMMA. They are derived from the fusion of skeletal myoblasts (MYOBLASTS, SKELETAL) into a syncytium, followed by differentiation.
Muscle Contraction
Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies. (1/79)
The primary aim of this study was to relate molecular and structural properties of in vitro reconstructed cardiac muscle with its electrophysiological function using an in vitro model system based on neonatal rat cardiac myocytes, three-dimensional polymeric scaffolds, and bioreactors. After 1 wk of cultivation, we found that engineered cardiac muscle contained a 120- to 160-microm-thick peripheral region with cardiac myocytes that were electrically connected through gap junctions and sustained macroscopically continuous impulse propagation over a distance of 5 mm. Molecular, structural, and electrophysiological properties were found to be interrelated and depended on specific model system parameters such as the tissue culture substrate, bioreactor, and culture medium. Native tissue and the best experimental group (engineered cardiac muscle cultivated using laminin-coated scaffolds, rotating bioreactors, and low-serum medium) were comparable with respect to the conduction velocity of propagated electrical impulses and spatial distribution of connexin43. Furthermore, the structural and electrophysiological properties of the engineered cardiac muscle, such as cellularity, conduction velocity, maximum signal amplitude, capture rate, and excitation threshold, were significantly improved compared with our previous studies. (+info)Peroxynitrite induced nitration and inactivation of myofibrillar creatine kinase in experimental heart failure. (2/79)
OBJECTIVE: Oxidative stress is implicated in the initiation and progression of congestive heart failure, but the putative reactive species and cellular targets involved remain undefined. We have previously shown that peroxynitrite (ONOO(-), an aggressive biological oxidant and nitrating agent) potently inhibits myofibrillar creatine kinase (MM-CK), a critical controller of contractility known to be impaired during heart failure. Here we hypothesized that nitration and inhibition of MM-CK participate in cardiac failure in vivo. METHODS: Heart failure was induced in rats by myocardial infarction (left coronary artery ligation) and confirmed by histological analysis at 8 weeks postinfarct (1.3+/-1.4 vs. 37.7+/-3.2% left ventricular circumference; sham control vs. CHF, n=10 each). RESULTS: Immunohistochemistry demonstrated significantly increased protein nitration in failing myocardium compared to control (optical density: 0.58+/-0.06 vs. 0.93+/-0.09, sham vs. CHF, P<0.05). Significant decreases in MM-CK activity and content were observed in failing hearts (MM-CK k(cat): 6.0+/-0.4 vs. 3.0+/-0.3 micromol/nM M-CK/min, P<0.05; 6.8+/-1.3 vs. 4.7+/-1.2% myofibrillar protein, P<0.05), with no change in myosin ATPase activity. In separate experiments, isolated rat cardiac myofibrils were exposed to ONOO(-) (2-250 microM) and enzyme studies were conducted. Identical to in vivo studies, selective reductions in MM-CK were observed at ONOO(-) concentrations as low as 2 microM (IC(50)=92.5+/-6.0 microM); myosin ATPase was unaffected with ONOO(-) concentrations as high as 250 microM. Concentration dependent nitration of MM-CK occurred and extent of nitration was statistically correlated to extent of CK inhibition (P<0.001). Immunoprecipitation of MM-CK from failing left ventricle yielded significant evidence of tyrosine nitration. CONCLUSION: These data demonstrate that cardiac ONOO(-) formation and perturbation of myofibrillar energetic controllers occur during experimental heart failure; MM-CK may be a critical cellular target in this setting. (+info)Production of recombinant human creatine kinase (r-hCK) isozymes by tandem repeat expression of M and B genes and characterization of r-hCK-MB. (3/79)
BACKGROUND: Serum creatine kinase-MB isoenzyme (CK-MB) is widely used as a marker of myocardial injury. We prepared recombinant human CK (r-hCK) MB isoenzyme and examined its potential for use as a control material for assay of CK-MB in serum. METHODS: cDNAs encoding CK-M and CK-B subunits were inserted into the same plasmid vector, followed by transformation of Escherichia coli. The resulting three types of CK isoenzymes were purified by conventional chromatography. RESULTS: The ratio of MB to MM to BB was 50:40:10 on the basis of CK activity. Highly purified CK-MB with a specific activity of 533 U/mg was produced in a yield of 5.7 mg/g of packed cells. Purified r-hCK-MB had the isoelectric point (pI 5.3) and molecular size (46 kDa for the subunit) of native CK-MB. Its immunoreactivity in an ELISA using antibody against native heart enzyme was similar to that of cardiac CK-MB. The r-hCK-MB retained >90% activity for at least 4 months at 11 degrees C in a delipidated serum matrix in a liquid form at a concentration of 118 U/L. CONCLUSIONS: r-hCK-MB shows key properties of the native cardiac isoenzyme and may be useful as a control and calibrator for serum assays of CK-MB. (+info)Enhanced expression of the alpha 7 beta 1 integrin reduces muscular dystrophy and restores viability in dystrophic mice. (4/79)
Muscle fibers attach to laminin in the basal lamina using two distinct mechanisms: the dystrophin glycoprotein complex and the alpha 7 beta 1 integrin. Defects in these linkage systems result in Duchenne muscular dystrophy (DMD), alpha 2 laminin congenital muscular dystrophy, sarcoglycan-related muscular dystrophy, and alpha 7 integrin congenital muscular dystrophy. Therefore, the molecular continuity between the extracellular matrix and cell cytoskeleton is essential for the structural and functional integrity of skeletal muscle. To test whether the alpha 7 beta 1 integrin can compensate for the absence of dystrophin, we expressed the rat alpha 7 chain in mdx/utr(-/-) mice that lack both dystrophin and utrophin. These mice develop a severe muscular dystrophy highly akin to that in DMD, and they also die prematurely. Using the muscle creatine kinase promoter, expression of the alpha 7BX2 integrin chain was increased 2.0-2.3-fold in mdx/utr(-/-) mice. Concomitant with the increase in the alpha 7 chain, its heterodimeric partner, beta 1D, was also increased in the transgenic animals. Transgenic expression of the alpha 7BX2 chain in the mdx/utr(-/-) mice extended their longevity by threefold, reduced kyphosis and the development of muscle disease, and maintained mobility and the structure of the neuromuscular junction. Thus, bolstering alpha 7 beta 1 integrin-mediated association of muscle cells with the extracellular matrix alleviates many of the symptoms of disease observed in mdx/utr(-/-) mice and compensates for the absence of the dystrophin- and utrophin-mediated linkage systems. This suggests that enhanced expression of the alpha 7 beta 1 integrin may provide a novel approach to treat DMD and other muscle diseases that arise due to defects in the dystrophin glycoprotein complex. A video that contrasts kyphosis, gait, joint contractures, and mobility in mdx/utr(-/-) and alpha 7BX2-mdx/utr(-/-) mice can be accessed at http://www.jcb.org/cgi/content/full/152/6/1207. (+info)Muscle-specific overexpression of the adenovirus primary receptor CAR overcomes low efficiency of gene transfer to mature skeletal muscle. (5/79)
Significant levels of adenovirus (Ad)-mediated gene transfer occur only in immature muscle or in regenerating muscle, indicating that a developmentally regulated event plays a major role in limiting transgene expression in mature skeletal muscle. We have previously shown that in developing mouse muscle, expression of the primary Ad receptor CAR is severely downregulated during muscle maturation. To evaluate how global expression of CAR throughout muscle affects Ad vector (AdV)-mediated gene transfer into mature skeletal muscle, we produced transgenic mice that express the CAR cDNA under the control of the muscle-specific creatine kinase promoter. Five-month-old transgenic mice were compared to their nontransgenic littermates for their susceptibility to AdV transduction. In CAR transgenics that had been injected in the tibialis anterior muscle with AdVCMVlacZ, increased gene transfer was demonstrated by the increase in the number of transduced muscle fibers (433 +/- 121 in transgenic mice versus 8 +/- 4 in nontransgenic littermates) as well as the 25-fold increase in overall beta-galactosidase activity. Even when the reporter gene was driven by a more efficient promoter (the cytomegalovirus enhancer-chicken beta-actin gene promoter), differential transducibility was still evident (893 +/- 149 versus 153 +/- 30 fibers; P < 0.001). Furthermore, a fivefold decrease in the titer of injected AdV still resulted in significant transduction of muscle (253 +/- 130 versus 14 +/- 4 fibers). The dramatic enhancement in AdV-mediated gene transfer to mature skeletal muscle that is observed in the CAR transgenics indicates that prior modulation of the level of CAR expression can overcome the poor AdV transducibility of mature skeletal muscle and significant transduction can be obtained at low titers of AdV. (+info)Overexpression of the LAR (leukocyte antigen-related) protein-tyrosine phosphatase in muscle causes insulin resistance. (6/79)
Previous reports indicate that the expression and/or activity of the protein-tyrosine phosphatase (PTP) LAR are increased in insulin-responsive tissues of obese, insulin-resistant humans and rodents, but it is not known whether these alterations contribute to the pathogenesis of insulin resistance. To address this question, we generated transgenic mice that overexpress human LAR, specifically in muscle, to levels comparable to those reported in insulin-resistant humans. In LAR-transgenic mice, fasting plasma insulin was increased 2.5-fold compared with wild-type controls, whereas fasting glucose was normal. Whole-body glucose disposal and glucose uptake into muscle in vivo were reduced by 39-50%. Insulin injection resulted in normal tyrosyl phosphorylation of the insulin receptor and insulin receptor substrate 1 (IRS-1) in muscle of transgenic mice. However, phosphorylation of IRS-2 was reduced by 62%, PI3' kinase activity associated with phosphotyrosine, IRS-1, or IRS-2 was reduced by 34-57%, and association of p85alpha with both IRS proteins was reduced by 39-52%. Thus, overexpression of LAR in muscle causes whole-body insulin resistance, most likely due to dephosphorylation of specific regulatory phosphotyrosines on IRS proteins. Our data suggest that increased expression and/or activity of LAR or related PTPs in insulin target tissues of obese humans may contribute to the pathogenesis of insulin resistance. (+info)Screening of dystrophin gene deletions in Egyptian patients with DMD/BMD muscular dystrophies. (7/79)
Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) are allelic disorders caused by mutations within the dystrophin gene. Our study has identified 100 Egyptian families collected from the Human Genetics Clinic, National Research Center, Cairo. All cases were subjected to complete clinical evaluation pedigree analysis, electromyography studies, estimation of serum creatine phosphokinase enzyme (CPK) levels and DNA analysis. Multiplex PCR using 18 pairs of specific primers were used for screening of deletion mutations within the dystrophin gene. A frequency of 55% among the families. Sixty per cent of detected deletions involved multiple exons spanning the major or the minor hot spot of the dystrophin gene. The remainder 40% which mainly involved exon 45. Comparing these findings with frequencies of other countries it was found that our figures fall within the reported range of 40%- distribution of deletions in our study and other different studies was variable and specific ethnic differences do not apparently account for specific deletions. In addition this study concluded that employment of the 18 exon analysis is a cost effective and a highly accurate (97% to launch a nationwide program. (+info)Electrophoresis of creatine kinase isoforms: a highly sensitive fluorescence scanning method. (8/79)
OBJECTIVE: To develop an agarose electrophoretic method for creatine kinase (CK) isoforms, using highly sensitive fluorescence scanning. METHODS: A discontinuous buffer system was used. Electrophoresis on agarose gel was performed under constant current and low voltage. CK isoforms were separated within 30 minutes and detected by fluorescence scanning. RESULTS: There were no significant differences when the activities of CK-MM were between 853.0 U/L and 14.0 U/L and those of CK-MB between 152.0 U/L and 2.4 U/L. The detection limits of stain method for CK-MM and CK-MB isoforms were 36.0 U/L and 12.3 U/L, respectively; while those of fluorescence method were 12.0 U/L and 2.1 U/L. The experimental results showed good precision for CK-MM isoforms, as well for CK-MB isoforms and isoenzymes. CONCLUSION: An agarose electrophoretic method has been developed to measure CK isoenzymes and isoforms clinically. This method is rapid, simple, sensitive, highly reproducible and inexpensive. It is suitable for general laboratories. (+info)
Conformationally Restricted Creatine Analogs and Substrate Specificity of Rabbit Muscle Creatine Kinase<...
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Altered calcium homeostasis and increased susceptibility to ischemia in creatine kinase-deficient hearts - Oxford...
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The mouse muscle creatine kinase promoter faithfully drives reporter gene expression in transgenic Xenopus laevis - Fingerprint...
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Probing into the role of conserved N-glycosylation sites in the Tyrosinase glycoprotein family [email protected]
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Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane...
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Creatine Kinase MB/Creatine Kinase Ratio
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Creatine Kinase (Blood)
Creatine Kinase (Blood)
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CKM (gene)
Creatine+Kinase,+MM+Form at the US National Library of Medicine Medical Subject Headings (MeSH) Human CKM genome location and ... "The generation of the oxidized form of creatine kinase is a negative regulation on muscle creatine kinase". J. Biol. Chem. 282 ... Creatine kinase, muscle also known as MCK is a creatine kinase that in humans is encoded by the MCK gene. In the figure to the ... 2007). "CK-MM and ACE genotypes and physiological prediction of the creatine kinase response to exercise". J. Appl. Physiol. ...
Anorexia nervosa
Creatine Kinase Test (CK-Test): measures the circulating blood levels of creatine kinase an enzyme found in the heart (CK-MB), ... The lifetime rate of atypical anorexia nervosa, a form of ED-NOS in which not all of the diagnostic criteria for AN are met, is ... brain (CK-BB) and skeletal muscle (CK-MM).[88]. *Blood urea nitrogen (BUN) test: urea nitrogen is the byproduct of protein ... Walder A, Baumann P (2008). "Increased creatinine kinase and rhabdomyolysis in anorexia nervosa". The International Journal of ...
Creatine
... itself can be phosphorylated by creatine kinase to form phosphocreatine, which is used as an energy buffer in skeletal ... The phosphate group is attached to an NH center of the creatine. In skeletal muscle, PCr concentrations may reach 20-35 mM or ... A cyclic form of creatine, called creatinine, exists in equilibrium with its tautomer and with creatine. Creatine is ... Persky AM, Rawson ES (2007). "Safety of creatine supplementation". Creatine and Creatine Kinase in Health and Disease. ...
Creatine kinase
1999 [5]). Cytosolic BB-CK, as well as muscle-type MM-CK are both forming banana-shaped symmetric dimers, with one active site ... Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phosphocreatine kinase, is an enzyme (EC 2.7.3.2) expressed ... Creatine kinase in the blood may be high in health and disease. Exercise increases the outflow of creatine kinase to the blood ... Thus creatine kinase is an important enzyme in such tissues.[3] Clinically, creatine kinase is assayed in blood tests as a ...
Duchenne muscular dystrophy
Creatine kinase (CPK-MM) levels in the bloodstream are extremely high. An electromyography (EMG) shows that weakness is caused ... Form-fitting removable leg braces that hold the ankle in place during sleep can defer the onset of contractures. Appropriate ... Those affected also have a high level of creatine kinase in their blood. Although there is no known cure, physical therapy, ... People with Becker's muscular dystrophy, which is milder than DMD, have a form of dystrophin which is functional even though it ...
Neuroleptic malignant syndrome
Latham J, Campbell D, Nichols W, Mott T (2008). "How much can exercise raise creatine kinase level-and does it matter?". The ... Use of high-potency neuroleptics, a rapid increase in the dosage of neuroleptics, and use of long-acting forms of neuroleptics ... Dhib-Jalbut S, Hesselbrock R, Mouradian MM, Means ED (1987). "Bromocriptine treatment of neuroleptic malignant syndrome". The ... The raised white blood cell count and creatine phosphokinase (CPK) plasma concentration seen in those with NMS is due to ...
Inclusion body myositis
Elevated creatine kinase (CK) levels in the blood (at most ~10 times normal) are typical in sIBM but affected individuals can ... Since these forms do not show features of muscle inflammation, they are classified as myopathies rather than forms of myositis ... IBMmyositis.com cureibm.org Machado, P; Dimachkie, MM; Bahron, RJ (October 2014). "Sporadic Inclusion Body Myositis: new ... Other forms of muscular dystrophy (e.g. limb girdle) must be considered as well.[citation needed] The common type is sIBM; it ...
Congenital myopathy
Tests can be run to check creatine kinase in the blood, which is often normal or mildly elevated in congenital myopathies. ... In its severest form, affected babies often die from respiratory failure. To date, 9 gene mutations have been found to cause ... Ryan MM, Schnell C, Strickland CD, et al. (September 2001). "Nemaline myopathy: a clinical study of 143 cases". Annals of ... Diagnosis usually relies on this method, as creatine kinase levels and electromyography can be unreliable and non-specific. ...
Ion chromatography
Mercer, Donald W (1974). "Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography". ... Firstly, CK- MM was eluted, then CK-MB, followed by CK-BB. Therefore, the isoenzymes found in each sample could be used to ... This competition led to fast growth of new forms and the fast evolution of IC.[11] A challenge that needs to be overcome in the ... A buffered aqueous solution known as the mobile phase carries the sample from the loop onto a column that contains some form of ...
Exercise intolerance
... releasing intracellular muscle content into the blood as reflected by elevated blood levels of creatine kinase. Exercise ... 200 mm Hg or resting diastolic blood pressure >110 mm Hg Severe pulmonary hypertension chronic fatigue syndrome Suspected or ... Chronic pain that makes a person unwilling to undertake a physical activity is not, by itself, a form of exercise intolerance. ...
List of MeSH codes (D08)
... creatine kinase, mitochondrial form MeSH D08.811.913.696.640.150.875 - creatine kinase, mm form MeSH D08.811.913.696.645 - ... creatine kinase MeSH D08.811.913.696.640.150.500 - creatine kinase, bb form MeSH D08.811.913.696.640.150.625 - creatine kinase ... map kinase kinase kinase 1 MeSH D08.811.913.696.620.682.700.559.200 - map kinase kinase kinase 2 MeSH D08.811.913.696.620.682. ... map kinase kinase kinase 3 MeSH D08.811.913.696.620.682.700.559.400 - map kinase kinase kinase 4 MeSH D08.811.913.696.620.682. ...
Carnitine palmitoyltransferase II deficiency
Adult patients often have serum and/or urine screen positive for the presence of myoglobin and serum creatine kinase and ... Severe forms may have continual pain from general life activity. The adult form has a variable age of onset. The first ... Nelson DL and Cox MM (2005). "Fatty Acid Catabolism" in Lehninger Principles of Biochemistry, 4th Ed. New York: W.H. Freeman ... The neonatal form is the least common clinical presentation of this disorder and is almost invariably fatal in rapid fashion ...
King brown snake
... include muscle pain and weakness in the presence of an elevated creatine kinase (CK). King brown snake venom has some ... If the smoke resembled the mali, or immaterial form, of a snake, then the person would die, as the victim of a ragalk (sorcerer ... The incubating temperature has been recorded as between 22 and 32 °C (72 and 90 °F). The eggs average 40.1 mm (1 5⁄8 in) in ... Clade II corresponded to a lineage of large snakes found across Australia, clade III was a dwarf form from the Kimberley, and ...
Glycerol-3-phosphate dehydrogenase
substrate pages: glycerol 3-phosphate, dihydroxyacetone phosphate related topics: glycerol phosphate shuttle, creatine kinase, ... White MR, Khan MM, Deredge D, Ross CR, Quintyn R, Zucconi BE, Wysocki VH, Wintrode PL, Wilson GM, Garcin ED (Jan 2015). "A ... As a result, there is a tendency to form adipose tissue leading to an accumulation of fat that favors obesity. GPDH has also ... The reactions catalyzed by cytosolic (soluble) and mitochondrial GPDH are as follows: There are two forms of GPDH: The ...
Chest pain
Liver enzymes Creatine kinase (and CK-MB fraction in many hospitals) D-dimer (when suspicion for pulmonary embolism is present ... Wertli MM, Ruchti KB, Steurer J, Held U (November 2013). "Diagnostic indicators of non-cardiovascular chest pain: a systematic ... Another benign and harmless form of a sharp, localized chest pain often mistaken for heart disease. Breast conditions Herpes ... Burgstaller JM, Jenni BF, Steurer J, Held U, Wertli MM (2014-08-11). "Treatment efficacy for non-cardiovascular chest pain: a ...
Neosaxitoxin
Cheng HS, Chua SO, Hung JS, Yip KK (April 1991). "Creatine kinase MB elevation in paralytic shellfish poisoning". Chest. 99 (4 ... Nav channels form the basis for electrical excitability in animals. Nav channels evolved from Ca2+ channels and were present in ... Zhang MM, Gruszczynski P, Walewska A, Bulaj G, Olivera BM, Yoshikami D (July 2010). "Cooccupancy of the outer vestibule of ... other forms of aquatic animal life other than birds or mammals, and all mollusks; and incorporate the use of "ppm" as another ...
Atorvastatin
Myopathy with elevation of creatine kinase (CK, aka CPK)[unreliable medical source?] and rhabdomyolysis are the most serious ... In other countries, atorvastatin calcium is made in tablet form by generic medication makers under various brand names ... less than 1 mm in size). The FDA received no reports of injury from the contamination. Ranbaxy also issued recalls of bottles ... Interactions with clofibrate, fenofibrate, gemfibrozil, which are fibrates used in accessory therapy in many forms of ...
Neutrophil
Khan HA, Alhomida AS, Sobki SH, Moghairi AA, Koronki HE (2012). "Blood cell counts and their correlation with creatine kinase ... They form an essential part of the innate immune system, with their functions varying in different animals. They are formed ... Clark SR, Ma AC, Tavener SA, McDonald B, Goodarzi Z, Kelly MM, Patel KD, Chakrabarti S, McAvoy E, Sinclair GD, Keys EM, Allen- ... Trapping of bacteria may be a particularly important role for NETs in sepsis, where NETs are formed within blood vessels. ...
Enzyme
... is produced in inactive form as chymotrypsinogen in the pancreas and transported in this form to the stomach where it is ... Cox MM, Nelson DL (2013). "Chapter 6.2: How enzymes work". Lehninger Principles of Biochemistry (6th ed.). New York, N.Y.: W.H ... This is frequently seen in the main enzyme classes such as kinases. Artificial (in vitro) evolution is now commonly used to ... One example of an enzyme that has changed its activity is the ancestor of methionyl amino peptidase (MAP) and creatine ...
HK2
In a similar mechanism, the pro-apoptotic creatine kinase binds and opens VDAC in the absence of HK2.[8] An alternative model ... This gene encodes a 100-kDa, 917-residue enzyme with highly similar N- and C-terminal domains that each form half of the ... Lehto M, Xiang K, Stoffel M, Espinosa R, Groop LC, Le Beau MM, Bell GI (Dec 1993). "Human hexokinase II: localization of the ... kinase activity. • glucose binding. • catalytic activity. • protein binding. • fructokinase activity. • ATP binding. • ...
Myocardial infarction
Commonly used blood tests include troponin and less often creatine kinase MB. Treatment of an MI is time-critical. Aspirin is ... 2 mm (0,2 mV) for males or ≥1.5 mm (0.15 mV) for females or ≥1 mm (0.1 mV) in two other adjacent chest or limb leads. ST ... A collagen scar forms in their place. When an artery is blocked, cells lack oxygen, needed to produce ATP in mitochondria. ATP ... A cholesterol core forms as foam cells die. In response to growth factors secreted by macrophages, smooth muscle and other ...
Animal attacks in Latin America
... creatine kinase increase, metabolic acidosis and hematuria. The patient received 5 ampoules of serum, but the condition ... The documentation and specimen provided indicate a fish that was 133.5 mm in length and had a head with a diameter of 11.5 mm. ... The spots on the head and neck are generally solid, as are those on the tail, where they may merge to form a band. Jaguars ... these spiders grow 30-50 mm in length and 150 mm in wingspan, and are characterized by their aggressive behavior, in the 1970s ...
HK2
In a similar mechanism, the pro-apoptotic creatine kinase binds and opens VDAC in the absence of HK2. An alternative model ... Lehto M, Xiang K, Stoffel M, Espinosa R, Groop LC, Le Beau MM, Bell GI (Dec 1993). "Human hexokinase II: localization of the ... This gene encodes a 100-kDa, 917-residue enzyme with highly similar N- and C-terminal domains that each form half of the ... As an isoform of hexokinase and a member of the sugar kinase family, HK2 catalyzes the rate-limiting and first obligatory step ...
Facioscapulohumeral muscular dystrophy
Creatine kinase (CK) blood level is often ordered when muscle damage is suspected. CK is an enzyme found in muscle, leaking ... 1995 The terms FSHD1A and FSHD1B are introduced to describe 4q-linked and non-4q-linked forms of the disease. 1996 FSHD Region ... Tawil, R; Mah, JK; Baker, S; Wagner, KR; Ryan, MM; Sydney Workshop, Participants. (July 2016). "Clinical practice ... Casein kinase 1 (CK1) inhibition has been identified by Facio Therapies, a Dutch pharmaceutical company, to repress DUX4 ...
Exercise physiology
Lastly, adenylate kinase catalyzes a reaction by which 2 ADP are combined to form ATP and adenosine monophosphate (AMP). This ... but the most readily depleted of the above sources is the PCr system which utilizes the enzyme creatine kinase. This enzyme ... Todd, MM; Dunlop, BJ; Shapiro, HM; Chadwick, HC; Powell, HC (1981). "Ventricular fibrillation in the cat: A model for global ... The creation of AMP resulting from this reaction stimulates AMP-activated protein kinase (AMP kinase) which is the energy ...
Neutrophil
"Blood cell counts and their correlation with creatine kinase and C-reactive protein in patients with acute myocardial ... Any ANC , 1500 cells / mm3 is considered neutropenia, but ,500 cells / mm3 is considered severe.[46] There is also new research ... They are formed from stem cells in the bone marrow and differentiated into subpopulations of neutrophil-killers and neutrophil- ... Trapping of bacteria may be a particularly important role for NETs in sepsis, where NETs are formed within blood vessels.[35] ...
Atorvastatin
Myopathy with elevation of creatine kinase (CK, aka CPK)[39][unreliable medical source?] and rhabdomyolysis are the most ... Atorvastatin metabolism is primarily through cytochrome P450 3A4 hydroxylation to form active ortho- and parahydroxylated ... less than 1 mm in size). The FDA received no reports of injury from the contamination.[90] Ranbaxy also issued recalls of ... In other countries, atorvastatin calcium is made in tablet form by generic medication makers under various brand names ...
Anoreksi
Salvadori A, Fanari P, Ruga S, Brunani A, Longhini E (1992). «Creatine kinase and creatine kinase-MB isoenzyme during and after ... Kreatinkinase er et enzym som finnes i hjertet (CK-MB), i hjernen (CK-BB) og i skjelettmuskulaturen (CK-MM).[105][106] ... Lucka I, Cebella A (2004). «[Characteristics of the forming personality in children suffering from anorexia nervosa]». ... Mroczkowski MM, Redgrave GW, Miller NR, McCoy AN, Guarda AS (2010). «Reversible vision loss secondary to malnutrition in a ...
Citrate synthase
Cox DL, Nelson MM (2005). Lehninger Principles of Biochemistry (4th ed.). New York: W.H. Freeman. pp. 608−9. ISBN 978-0-7167- ... This forms a tetrahedral intermediate and results in the ejection of −SCoA as the carbonyl reforms. The −SCoA is protonated to ... Adenylate kinase. *Creatine kinase. Inner membrane. oxidative phosphorylation. *Coenzyme Q - cytochrome c reductase ... Only when this citroyl-CoA has formed will another conformational change cause thioester hydrolysis and release coenzyme A. ...
Glutamine synthetase
If PII is uridylylated, then it will take on the PIID form. The AT:PIID complex will activate GS by deadenylylation.[24] The AT ... Phosphorylation of NRI is catalyzed by NRII, a protein kinase. If NRII is complexed with PIIA then it will function as a ... Yamashita MM, Almassy RJ, Janson CA, Cascio D, Eisenberg D (October 1989). "Refined atomic model of glutamine synthetase at 3.5 ... Another form is cytosolic. The cytosolic GS gene translation is regulated by its 5' untranslated region (UTR), while its 3' UTR ...
एनोरेक्सिया नर्वोज़ा - विकिपीडिया
Salvadori A, Fanari P, Ruga S, Brunani A, Longhini E (1992). "Creatine kinase and creatine kinase-MB isoenzyme during and after ... Mroczkowski MM, Redgrave GW, Miller NR, McCoy AN, Guarda AS (2010). "Reversible vision loss secondary to malnutrition in a ... Lucka I, Cebella A (2004). "[Characteristics of the forming personality in children suffering from anorexia nervosa]". ... क्रियेटीन काइनेज़ टेस्ट (CK-टेस्ट): इसमें हृदय (CK-MB), मस्तिष्क (CK-BB) और मांसपेशियों (CK-MM) में पाए जाने वाले क्रियेटीन ...
Respiratory complex I
A-form and the other is the catalytically silent, dormant, "deactive", D-form. After exposure of idle enzyme to elevated, but ... Roessler MM, King MS, Robinson AJ, Armstrong FA, Harmer J, Hirst J (February 2010). "Direct assignment of EPR spectra to ... Adenylate kinase. *Creatine kinase. Inner membrane. oxidative phosphorylation. *Coenzyme Q - cytochrome c reductase ... The A-form of complex I is insensitive to sulfhydryl reagents. It was found that these conformational changes may have a very ...
Melatonin
Some of the metabolites formed via the reaction of melatonin with a free radical include cyclic 3-hydroxymelatonin, N1-acetyl- ... Zhdanova IV, Wurtman RJ, Regan MM, Taylor JA, Shi JP, Leclair OU (October 2001). "Melatonin treatment for age-related insomnia ... elevates the intracellular cAMP concentration via beta-adrenergic receptors and activates the cAMP-dependent protein kinase A ( ... 4a-peroxypterin could then react with the active site iron (II) to form an iron-peroxypterin intermediate or directly transfer ...
Amino acid synthesis
The loop formed by strands 2 and 3 forms an anti-terminator and translation of the his genes will continue and histidine will ... Nelson DL, Cox MM (2000). Lehninger, Principles of Biochemistry (3rd ed.). New York: Worth Publishing. ISBN 1-57259-153-6. .. ... Aspartate kinase becomes downregulated by the presence of threonine or lysine. Lysine[edit]. Lysine is synthesized from ... Instead strands 3 and 4 will form a hairpin loop further downstream of the ribosome. The hairpin loop formed by strands 3 and 4 ...
Methamphetamine
Form of neuroplasticity. or behavioral plasticity Type of reinforcer Sources Opiates Psychostimulants High fat or sugar food ... it triggers transporter phosphorylation via protein kinase A (PKA) and protein kinase C (PKC) signaling, ultimately resulting ... Mitler MM, Hajdukovic R, Erman MK (1993). "Treatment of narcolepsy with methamphetamine". Sleep. 16 (4): 306-317. PMC 2267865. ... such as a reduction in N-acetylaspartate and creatine levels and elevated levels of choline and myoinositol.[22] ...
ഓക്സിഡേറ്റീവ് ഫോസ്ഫോറിലേഷൻ - വിക്കിപീഡിയ
Reduction of coenzyme Q from its ubiquinone form (Q) to the reduced ubiquinol form (QH2). ... Nelson DL; Cox MM (2004). Lehninger Principles of Biochemistry (4th ed.). W. H. Freeman. ISBN 0-7167-4339-6. .. ... regulated by Pyruvate dehydrogenase kinase and Pyruvate dehydrogenase phosphatase). to α-ketoglutaric acid. *Glutamate ... Creatine. & Polyamines Ketogenic &. Glucogenic. Amino Acids Amino Acids Shikimate Aromatic Amino. Acids & Histidine ...
ಅನೋರೆಕ್ಸಿಯಾ ನರ್ವೋಸಾ - ವಿಕಿಪೀಡಿಯ
Salvadori A, Fanari P, Ruga S, Brunani A, Longhini E (1992). "Creatine kinase and creatine kinase-MB isoenzyme during and after ... Mroczkowski MM, Redgrave GW, Miller NR, McCoy AN, Guarda AS (2010). "Reversible vision loss secondary to malnutrition in a ... Lucka I, Cebella A (2004). "[Characteristics of the forming personality in children suffering from anorexia nervosa]". ... Walder A, Baumann P (2008). "Increased creatinine kinase and rhabdomyolysis in anorexia nervosa". The International Journal of ...
Chest pain
Creatine kinase (and CK-MB fraction in many hospitals). *D-dimer (when suspicion for pulmonary embolism is present but low) ... Wertli MM, Ruchti KB, Steurer J, Held U (November 2013). "Diagnostic indicators of non-cardiovascular chest pain: a systematic ... Precordial catch syndrome - another benign and harmless form of a sharp, localized chest pain often mistaken for heart disease ... Burgstaller JM, Jenni BF, Steurer J, Held U, Wertli MM (2014-08-11). "Treatment efficacy for non-cardiovascular chest pain: a ...
Transfer RNA
Once the peptide bond is formed, the tRNA in the P/P site is deacylated, or has a free 3' end, and the tRNA in the A/A site ... Emara MM, Ivanov P, Hickman T, Dawra N, Tisdale S, Kedersha N, Hu GF, Anderson P (April 2010). "Angiogenin-induced tRNA-derived ... Adenylate kinase. *Creatine kinase. Inner membrane. oxidative phosphorylation. *Coenzyme Q - cytochrome c reductase ... The anticodon forms three complementary base pairs with a codon in mRNA during protein biosynthesis. On the other end of the ...
Caffeine
Snel J, Lorist MM (2011). "Effects of caffeine on sleep and cognition". Progress in Brain Research. 190: 105-17. doi:10.1016/ ... Caffeine can produce a mild form of drug dependence - associated with withdrawal symptoms such as sleepiness, headache, and ... S2CID 378245.CS1 maint: uses authors parameter (link) Tarnopolsky MA (2010). "Caffeine and creatine use in sport". Annals of ... As a competitive nonselective phosphodiesterase inhibitor, caffeine raises intracellular cAMP, activates protein kinase A, ...
Anti-Creatine Kinase MM antibody (ab233201) | Abcam
Rabbit polyclonal Creatine Kinase MM antibody. Validated in WB, IHC and tested in Pig. Immunogen corresponding to recombinant ... Form. Liquid. * Storage instructions. Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20 ... Anti-Creatine Kinase MM antibody (ab233201) at 2 µg/ml + Recombinant pig Creatine Kinase MM protein.. Predicted band size: 43 ... Anti-Creatine Kinase MM antibody. See all Creatine Kinase MM primary antibodies. ...
Anti-Creatine Kinase MM antibody (Biotin) (ab48652) | Abcam
Rabbit polyclonal Creatine Kinase MM antibody conjugated to Biotin. Validated in WB, IP, ELISA, RIA and tested in Human. ... Anti-Creatine Kinase MM antibody (Biotin). See all Creatine Kinase MM primary antibodies. ... Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase ... Form. Liquid. * Storage instructions. Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid ...
Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres
Creatine kinase-mediated improvement of function in failing mouse hearts provides causal evidence the failing heart is energy...
Despite experimental and clinical HF data showing reduced metabolism through cardiac creatine kinase (CK), the maj … ... Creatine Kinase, MM Form / genetics * Creatine Kinase, MM Form / metabolism* * Disease Models, Animal ... Creatine kinase-mediated improvement of function in failing mouse hearts provides causal evidence the failing heart is energy ... Despite experimental and clinical HF data showing reduced metabolism through cardiac creatine kinase (CK), the major myocardial ...
Creatine Kinase MM Antibody (PA5-86374)
Invitrogen Anti-Creatine Kinase MM Polyclonal, Catalog # PA5-86374. Tested in Western Blot (WB), Immunofluorescence (IF) and ... Protein Aliases: CPK-M; Creatine kinase M chain; Creatine kinase M-type; creatine kinase, muscle; Creatine phosphokinase M-type ... Cite Creatine Kinase MM Polyclonal Antibody. The following product was used in this experiment: Creatine Kinase MM Polyclonal ... Creatine Kinase MM Polyclonal Antibody. View all (18) Creatine Kinase MM antibodies ...
Creatine Kinase MM (CK-MM), Human Heart | LeeBio.com
Buy Creatine Kinase MM from human cardiac tissue - E.C. 2.7.3.2 - Lyophilized Powder - For use in research and diagnostic ... Form. Lyophilized. Activity. > 200 U/mg (Dimension® Clinical Chemistry System). Unit Definition. One unit will catalyze the ... Lee Biosolutions is the leading manufacturer of human Creatine Kinase MM (CK-MM) isoenzyme for medical research and diagnostic ... Home / Antigens / Enzymes / Enzymes / Standards / Controls / Proteins / Creatine Kinase Mm (ck-mm) ...
Creatine Kinase MM (CK-MM), Human Skeletal | LeeBio.com
CK-MM) from human skeletal muscle for diagnostic manufacturing, control, calibrator and research uses - Inquire for details! ... Creatine Kinase MM. Source. Human Skeletal Muscle. Catalogue Number. 190-29. Form. 50% Glycerol. ... Home / Antigens / Enzymes / Antigens / Enzymes / Standards / Controls / Proteins / Creatine Kinase Mm (ck-mm) ... Lee Biosolutions is the leading manufacturer of human muscle Creatine Kinase MM (CKMM,CK-MM) for medical research and ...
Creatine kinase - Wikipedia
1999 [5]). Cytosolic BB-CK, as well as muscle-type MM-CK are both forming banana-shaped symmetric dimers, with one active site ... Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phosphocreatine kinase, is an enzyme (EC 2.7.3.2) expressed ... Creatine kinase in the blood may be high in health and disease. Exercise increases the outflow of creatine kinase to the blood ... Thus creatine kinase is an important enzyme in such tissues.[3] Clinically, creatine kinase is assayed in blood tests as a ...
What are the signs of elevated creatine kinase? | Reference.com
Signs and symptoms that typically accompany an elevated creatine kinase include cardiac symptoms such as chest pain, breathing ... CK-MM is a form of creatine kinase found in the heart and skeletal muscles, whereas CK-MB is present in the heart. The type of ... creatine kinase located in the brain is called CK-BB.. A higher creatine kinase level usually manifests a few hours after a ... The heart, brain and skeletal muscles are the parts of the body that primarily contain the enzyme creatine kinase, states ...
Creatine kinase - wikidoc
In addition to those, there are two mitochondrial creatine kinases, the ubiquitous and sarcomeric form. ... Skeletal muscle expresses CK-MM (98%) and low levels of CK-MB (1%). The myocardium (heart muscle), in contrast, expresses CK-MM ... Creatine kinase (CK), also known as phosphocreatine kinase or creatine phosphokinase (CPK) is an enzyme (EC 2.7.3.2) expressed ... Thus Creatine Kinase is an important enzyme in such tissues. Clinically, creatine kinase is assayed in blood tests as a marker ...
CKM - Creatine kinase M-type - Canis lupus familiaris (Dog) - CKM gene & protein
... creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating ... With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, ... PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. ... PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. ...
CKM - Creatine kinase M-type - Homo sapiens (Human) - CKM gene & protein
... creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating ... Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form ... PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. ... PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. ...
Familial End Stage Hypertrophic Cardiomyopathy
Laboratory investigations showed increased plasma transaminases and creatine kinase (CK) levels. At the age of 14 years, he ... The evolution of HCM to the dilated hypokinetic form (end stage disease) was very fast. The patient died at the age of 17 years ... His Holter monitoring revealed burst of non-sustained ventricular tachycardia (NSVT) and his LVWmax reached 30 mm. ... Patient B, 5 years older than patient A, was diagnosed with HCM (non obstructive form) at the age of 9 years. Ten years later ...
Anti-CKM / Creatine Kinase MM Antibody | Mouse anti-Human ELISA | LSBio
Creatine Kinase MM antibody LS-C14686 is an unconjugated mouse monoclonal antibody to human Creatine Kinase MM (CKM). Validated ... Creatine Kinase MM antibody LS-C14686 is an unconjugated mouse monoclonal antibody to human Creatine Kinase MM (CKM). Validated ... Creatine Kinase MM antibody LS-C14686 is an unconjugated mouse monoclonal antibody to human Creatine Kinase MM (CKM). Validated ... Recognizes Creatine Kinase MM Isoenzyme (CKMM). This antibody does not react with CKBB. Cross-reacts with CKMB: ~52%. ...
CKM (gene) - Wikipedia
Creatine+Kinase,+MM+Form at the US National Library of Medicine Medical Subject Headings (MeSH) Human CKM genome location and ... "The generation of the oxidized form of creatine kinase is a negative regulation on muscle creatine kinase". J. Biol. Chem. 282 ... Creatine kinase, muscle also known as MCK is a creatine kinase that in humans is encoded by the MCK gene. In the figure to the ... 2007). "CK-MM and ACE genotypes and physiological prediction of the creatine kinase response to exercise". J. Appl. Physiol. ...
Creatine-Kinase- and Exercise-Related Muscle Damage Implications for Muscle Performance and Recovery
The appearance of creatine kinase (CK) in blood has been generally considered to be an indirect marker of muscle damage, ... MM and 20-30% MB, while brain has predominantly BB. In mitochondria there are two specific forms of mitochondrial CK (Mt-CK): a ... Potential roles of adenylate kinase (AK) and AMP-activated protein kinase (AMPK) in gross control of creatine kinase (CK) ... "CM-MM and ACE genotypes and physiological prediction of the creatine kinase response to exercise," Journal of Applied ...
Alcohol-Related Neurologic Disease | Encyclopedia.com
... the MM fraction of creatine kinase.. The severe form of acute alcoholic myopathy is associated with the sudden onset of muscle ... In chronic alcoholic myopathy, serum creatine kinase often is normal, and muscle biopsy shows atrophy, or loss of muscle fibers ... Acute alcoholic myopathy can be diagnosed by finding myoglobin in the urine and increased creatine kinase and other blood ... In its mildest form, this breakdown may cause no noticeable symptoms, but may be detected by a temporary elevation in blood ...
Anorexia nervosa - Wikipedia
Creatine Kinase Test (CK-Test): measures the circulating blood levels of creatine kinase an enzyme found in the heart (CK-MB), ... The lifetime rate of atypical anorexia nervosa, a form of ED-NOS in which not all of the diagnostic criteria for AN are met, is ... brain (CK-BB) and skeletal muscle (CK-MM).[88]. *Blood urea nitrogen (BUN) test: urea nitrogen is the byproduct of protein ... Walder A, Baumann P (2008). "Increased creatinine kinase and rhabdomyolysis in anorexia nervosa". The International Journal of ...
Anti-CKMB / Creatine Kinase MB Antibody (Azide-free) LS-C193283
... that binds human creatine Kinase MB (also known as CKMB). ... Creatine Kinase MB antibody LS-C193283 is a protein G-purified ... there are two mitochondrial creatine kinase isoenzymes, the ubiquitous and sarcomeric form. (More About CKMB / Creatine Kinase ... CK-MM, CK-BB and CK-MB. The genes for these subunits are located on different chromosomes: B on 14q32 and M on 19q13. In ... Most Popular CKMB / Creatine Kinase MB Antibodies. Anti-CKMB / Creatine Kinase MB Antibody LS-C14666 ...
Masticatory Muscle Myositis (MMM) in Dogs - PetPlace
MMM) is an inflammatory condition involving the muscles of mastication or chewing in the dog. Learn more about MMM here. ... Serum creatine kinase. *Serum type 2M myosin antibody titer. *Muscle biopsy and immunocytochemistry ... The disease occurs in both acute (sudden) and chronic forms, and the signs may vary with each form. The chronic form is seen ... Masticatory muscle myositis (MMM) is an inflammatory condition involving the muscles of mastication or chewing in the dog. MMM ...
Genetic and Molecular Basis of Cardiac Arrhythmias: Impact on Clinical Management Parts I and II | Circulation
Other candidate genes within the mapped region include apolipoprotein C2, creatine kinase-MM, myotonic dystrophy, troponin T, ... a more common autosomal dominant form with a pure cardiac phenotype (Romano-Ward3 ) and a rarer autosomal recessive form ... and the histidine-rich Ca2+-binding protein (a luminal sarcoplasmic reticulum protein). Myotonic dystrophy, creatine kinase-MM ... The second form of PFHB, known as PFHB-II, presents with complete heart block and narrow QRS complexes and is believed to occur ...
Unruptured left ventricular pseudoaneurysm following myocardial infarction | Heart
... peak MB creatine kinase ⩾ 150 IU/l, not having a mural thrombus, lack of coronary artery collateral vessels in the area of ... Hypotension (blood pressure 85/45 mm Hg) developed two days later and he was managed with aspirin, isosorbide dinitrate, and ... up to 3746 IU/l with MB form of 8.5%). There was no evidence of right ventricular infarction by initial right sided ... myocardial infarction was diagnosed by typical evolutional changes in a 12 lead electrocardiogram and raised creatine kinase ( ...
Detection of reperfusion within 1 hour after coronary recanalisation by analysis of isoforms of the MM creatine kinase...
Together they form a unique fingerprint. * MM Form Creatine Kinase Medicine & Life Sciences ... T1 - Detection of reperfusion within 1 hour after coronary recanalisation by analysis of isoforms of the MM creatine kinase ... title = "Detection of reperfusion within 1 hour after coronary recanalisation by analysis of isoforms of the MM creatine kinase ... Detection of reperfusion within 1 hour after coronary recanalisation by analysis of isoforms of the MM creatine kinase ...
The mouse muscle creatine kinase promoter faithfully drives reporter gene expression in transgenic Xenopus laevis - Fingerprint...
Chronic renal failure|Neurobehavioral changes|Erythropoietin
The creatine kinase activity in brain regions was expressed as μmol of creatine formed/min./mg protein. The creatine kinase ... containing 7 mM phosphocreatine, 9 mM MgSO4, and approximately 1 microgram protein in a final volume of 0.13 mL. After 20 min ... Determination of creatine kinase (CK) activity. The activity of creatine kinase was assayed spectrophotometrically in serum and ... Hughes BP (1962) A method for the estimation of serum creatine kinase and its use in comparing creatine kinase and aldolase ...
CK MB Rapid Test | CE-GMP | 818-591 3030-USA.
Creatine kinase is a dimer occurring in various in three isoenzymic forms, depending on the particular combination of its non- ... identical subunits: BB (brain type); MM (skeletal type); and MB(hybrid type). Creatine kinase-MB isoenzyme is released into ... The sample continues to move to the control area and forms a pink to purple color, indicating the test is working and the ...
Distinct Forms of Mitochondrial TOM-TIM Supercomplexes Define Signal-Dependent States of Preprotein Sorting | Molecular and...
2 mM ATP, and an ATP-regenerating system (5 mM creatine phosphate and 0.1 mg/ml creatine kinase) at 25°C for 15 to 30 min ... The import buffer contained 3% (wt/vol) BSA, 250 mM sucrose, 80 mM KCl, 5 mM MgCl2, 5 mM KPi, 5 mM methionine, and 10 mM MOPS- ... 20 mM HEPES-KOH [pH 8.0], 100 mM NaCl, 10% [vol/vol] glycerol, 40 mM imidazole, 2 mM PMSF, 0.3% [wt/vol] digitonin), and bound ... After the column was washed, proteins were eluted with elution buffer (20 mM Tris-HCl, 200 mM NaCl, and 400 mM imidazole, pH ...
Tight linkage of creatine kinase (CKMM) to myotonic dystrophy on chromosome 19<...
MM Form Creatine Kinase Medicine & Life Sciences * Prenatal Diagnosis Medicine & Life Sciences ... Tight linkage of creatine kinase (CKMM) to myotonic dystrophy on chromosome 19. Together they form a unique fingerprint. * ... Tight linkage of creatine kinase (CKMM) to myotonic dystrophy on chromosome 19. / Yamaoka, L. H.; Pericak-Vance, M. A.; Speer, ... There have been reports of tight linkage to a number of chromosome 19 markers, including APOC2 and creatine kinase muscle type ...
High Purity/Activity Enzymes | LeeBio.com
Creatine Kinase MM (CK-MM) Catalog No: 190-28 Form: Lyophilized 190-28. Human Skeletal Muscle. Highly Purified. Lyophilized. ... Creatine Kinase MM (CK-MM) Catalog No: 190-29 Form: 50% Glycerol ... Creatine Kinase MM (CK-MM) Catalog No: 190-26 Form: Lyophilized ... Creatine Kinase MB (CK-MB) Catalog No: 190-25 Form: Lyophilized 190-25. Human Heart. Purified (Control Grade). Lyophilized. ... Creatine Kinase MB (CK-MB), Type I Catalog No: 190-24-R1 Form: Liquid ...
Frontiers | Development and regulation of chloride homeostasis in the central nervous system | Cellular Neuroscience
... topographical interaction with the Na+-K+ ATPase and its modulators such as creatine kinase should modulate functions of Cl- ... In addition, activation of these kinases is modulated by humoral factors such as estrogen and taurine. Because these ... KCC2 and NKCC1 functions are also regulated by phosphorylation by enzymes such as PKC, Src-family tyrosine kinases, and WNK1-4 ... the predominant form in brain), MM (in skeletal muscle) and MB (in cardiac muscle). Of these, only CKB interacts with KCC2; ...
IsoenzymesSkeletal muscleMuscleCKMMPhosphokinaseFingerprintAntibodySerumDimericType of creatine kinasePhosphateCardiacIsoenzyme of creatineEnzyme creatine kinaseHigher creatine kinaseSubunitsProteinsAbstractCytosolicProtein kinaseGeneAdenosineTissuesDifferentiating patientsBrainMyocardialHeart and skeletal musclesPhosphorylationIsoformActivityDeterminationMusclesRecombinantAcuteHUMANDiagnosticSignificanceMuscularXenopus
Isoenzymes18
- Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. (abcam.com)
- In addition to those three cytosolic CK isoforms, there are two mitochondrial creatine kinase isoenzymes, the ubiquitous and sarcomeric form. (wikipedia.org)
- These subunits allow the formation of three tissue-specific isoenzymes: CK-MB (cardiac muscle), CK-MM (skeletal muscle), and CK-BB (brain). (hindawi.com)
- Creatine kinase (CK) and CK isoenzymes are known to fluctuate in labor. (utmb.edu)
- Scott, R. T. / Creatine kinase and creatine kinase isoenzymes as a marker of uterine activity . (utmb.edu)
- Other isoenzymes include CK MM and CK BB. (1mg.com)
- All isoenzymes of CK catalyze the phosphorylation of creatine to form phosphocreatine. (aaltoscientific.com)
- The Ontogeny of Creatine Kinase Isoenzymes. (aaltoscientific.com)
- Creatine Kinase Isoenzymes in Cerebrospinal Fluid in a Case of Brain Damage. (aaltoscientific.com)
- The Expression of Creatine Kinase Isoenzymes in Neocortex of Patients with Neurodegenerative Disorders: Alzheimer's and Pick's Disease. (aaltoscientific.com)
- Creatine kinase isoenzymes from Torpedo californica electric organ, skeletal muscle, and brain were purified and characterized. (mysciencework.com)
- Localization of creatine kinase isoenzymes in myofibrils. (rupress.org)
- The subunits combine to form three distinct CK isoenzymes, CK-BB (CK-1), CK-MB (CK-2) and CK-MM (CK-3). (vitroscient.com)
- Creatine Kinase exists as dimeric molecules composed of M and B subunits that form the isoenzymes MM, MB, and BB. (pointescientific.com)
- Creatine Kinase (CK) is a dimeric enzyme that catalyzes the conversion of creatine to creatine phosphate, it is composed of two sub-units Muscle (M) and Brain (B) which associate to form three isoenzymes CK-MM, CK-MB and CK-BB. (randoxonlinestore.com)
- Isoenzymes that catalyze the same physiologic reaction may also appear in different forms in different animal species. (nurseslearning.com)
- All three of these isoenzymes make up the main enzyme CPK (creatine phosphokinase) (also called CK--creatine kinase). (nurseslearning.com)
- Creatine Kinase MB (CK-MB) is an enzyme present in the cardiac muscle with a molecular weight of 87.0 kDa.1Creatine Kinase is a dimeric molecule formed from two subunits designated as "M" and "B" which combine to form three different isoenzymes, CK-MM, CK-BB, and CK-MB. (custom-monoclonalantibody.com)
Skeletal muscle15
- When human CK-MM isoenzyme is elevated, this usually indicates injury or stress to skeletal muscle. (leebio.com)
- Typically, the ratio of subunits varies with muscle type: skeletal muscle: 98% MM and 2% MB and cardiac muscle: 70-80% MM and 20-30% MB, while brain has predominantly BB. (hindawi.com)
- In mitochondria there are two specific forms of mitochondrial CK (Mt-CK): a nonsarcomeric type called ubiquitous Mt-CK expressed in various tissues such as brain, smooth muscle, and sperm, and a sarcomeric Mt-CK expressed in cardiac and skeletal muscle [ 1 ]. (hindawi.com)
- Faster O₂ uptake kinetics in canine skeletal muscle in situ after acute creatine kinase inhibition. (nih.gov)
- Creatine kinase (CK) plays a key role both in energy provision and in signal transduction for the increase in skeletal muscle O2 uptake () at exercise onset. (nih.gov)
- Oxidative modification and aggregation of creatine kinase from aged mouse skeletal muscle. (utmb.edu)
- Fingerprint Dive into the research topics of 'Oxidative modification and aggregation of creatine kinase from aged mouse skeletal muscle. (utmb.edu)
- We have blocked creatine kinase (CK) mediated phosphocreatine (PCr) ⇆ ATP transphosphorylation in mitochondria and cytosol of skeletal muscle by knocking out the genes for the mitochondrial (ScCKmit) and the cytosolic (M-CK) CK isoforms in mice. (elsevier.com)
- Very high levels of CK are found in skeletal muscle, primarily the MM form. (aaltoscientific.com)
- Torpedo electric organ and skeletal muscle creatine kinase have identical apparent Mr, electrophoretic mobility, and cyanogen bromide fragments. (mysciencework.com)
- Western blot analysis showed Torpedo electric organ skeletal muscle creatine kinase and acetylcholine receptor-enriched membrane creatine kinase reacted with antiserum specific for canine MM creatine kinase. (mysciencework.com)
- This tissue thus provides an interesting contrast to skeletal muscle, in which some of the MM-CK present as predominant CK isoenzyme is bound at the myofibrillar M-line. (rupress.org)
- With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. (abcam.cn)
- CK-MM is the predominant form of CK in skeletal muscle. (vitroscient.com)
- Creatine kinase isoenzyme activities in extracts of plasma, skeletal muscle, heart and brain tissue of domestic fowls were separated by anion exchange chromatography and tissue specific distributions of the isoenzyme designated MM-CK, BB-CK1 and BB-CK2 were demonstrated. (sruc.ac.uk)
Muscle53
- When PGC-1 alpha is expressed at physiological levels in transgenic mice driven by a muscle creatine kinase (MCK) promoter, a fibre type conversion is observed: muscles normally rich in type II fibres are redder and activate genes of mitochondrial oxidative metabolism. (nih.gov)
- Lee Biosolutions is the leading manufacturer of human muscle Creatine Kinase MM (CKMM,CK-MM) for medical research and diagnostic manufacturing. (leebio.com)
- Clinically, creatine kinase is assayed in blood tests as a marker of damage of CK-rich tissue such as in myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy , autoimmune myositides , and acute kidney injury . (wikipedia.org)
- The first X-ray structure of a creatine kinase (CK) family member solved was that of sarcomeric muscle-type mitochondrial CK (s-mtCK (in 1996) (Fritz-Wolf et al. (wikipedia.org)
- Cytosolic BB-CK, as well as muscle-type MM-CK are both forming banana-shaped symmetric dimers, with one active site in each subunit. (wikipedia.org)
- Apart from the two mitochondrial CK isoenzyme forms, that is, ubiquitous mtCK (present in non-muscle tissues) and sarcomeric mtCK (present in sarcomeric muscle), there are three cytosolic CK isoforms present in the cytosol, depending on the tissue. (wikipedia.org)
- Whereas MM-CK is expressed in sarcomeric muscle, that is, skeletal and cardiac muscle, MB-CK is expressed in cardiac muscle, and BB-CK is expressed in smooth muscle and in most non-muscle tissues. (wikipedia.org)
- A higher creatine kinase level usually manifests a few hours after a heart attack when the heart muscle cells start to die, says Healthgrades. (reference.com)
- Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy and in acute renal failure . (wikidoc.org)
- Creatine kinase, muscle also known as MCK is a creatine kinase that in humans is encoded by the MCK gene. (wikipedia.org)
- Its functional entity is a MM-CK homodimer in striated (sarcomeric) skeletal and cardiac muscle. (wikipedia.org)
- In heart, in addition to the MM-CK homodimer, also the heterodimer MB-CK consisting of one muscle (M-CK) and one brain-type (B-CK) subunit is expressed. (wikipedia.org)
- The appearance of creatine kinase (CK) in blood has been generally considered to be an indirect marker of muscle damage, particularly for diagnosis of medical conditions such as myocardial infarction, muscular dystrophy, and cerebral diseases. (hindawi.com)
- Phosphocreatine (PCr) circuit showing the rephosphorylation of creatine (Cr) in mitochondria using ATP derived from oxidative phosphorylation (oxid phos) and subsequent use of mitochondrial PCr by cytosolic creatine kinase (CK) to resupply ATP for muscle activity, adapted from Saks [ 5 ]. (hindawi.com)
- Masticatory muscle myositis (MMM) is an inflammatory condition involving the muscles of mastication or chewing in the dog. (petplace.com)
- There have been reports of tight linkage to a number of chromosome 19 markers, including APOC2 and creatine kinase muscle type (CKMM), but they did not establish orientation of the 2 markers to DM. (elsevier.com)
- The severe form of the disease may present with muscle, heart, liver and central nervous system involvement. (biomedcentral.com)
- Enzyme expressed in muscle and other tissues that catalyses the conversion of creatine to phosphocreatine and adenosine diphosphate. (biologists.org)
- Miyoshi myopathy (MM) and limb girdle muscular dystrophy (LGMD2B) are distinct clinical entities because different muscle groups are involved at the onset. (neurologyindia.com)
- Patients with classical phenotype of MM and LGMD2B underwent a thorough phenotypic characterization followed by muscle histopathological study including IHC for dysferlin deficiency. (neurologyindia.com)
- 5 mm) on kinetics were studied in isolated canine gastrocnemius muscles in situ (n = 6) during transitions from rest to 3 min of electrically stimulated contractions eliciting ∼70% of muscle peak , and were compared to control (Ctrl) conditions. (nih.gov)
- The caregene study: muscle-specific creatine kinase gene and aerobic power in coronary artery disease. (cdc.gov)
- In 927 biologically unrelated Caucasian patients with coronary artery disease it was investigated whether the NcoI restriction fragment length polymorphism of the muscle-specific creatine kinase (CKMM) gene is associated with aerobic power and with the response to physical training. (cdc.gov)
- In addition to the macroscopic simulation, we analysed the buffering of bursts of high adenosine triphosphate hydrolysis by creatine kinase during cyclical muscle activity at the biochemical pathway level. (royalsocietypublishing.org)
- Three different iso-forms occur in normal circulation: CK-MM (muscle type), CK-BB (brain type) and CK-MB that is primarily associated with the myocardium. (southtees.nhs.uk)
- Muscle creatine kinase (CKm) activity maintains energetic homeostasis as variations in energy requirements dictate that ATP be readily available. (utmb.edu)
- This study examined the association between a DNA polymorphism in the muscle-specific creatine kinase (CKMM) gene and V̇O(2max) in the sedentary state, as well as its response (ΔV̇O(2max)) tO a standardized 20-wk endurance training program. (umn.edu)
- These results indicate that the NcoI polymorphism in the 3' untranslated region of the muscle-specific creatine kinase gene is associated with the ΔV̇O(2max) to endurance training. (umn.edu)
- An isoenzyme of creatine kinase found in the MUSCLE. (bvsalud.org)
- CK-MM rises with other muscle damage. (lomalindahealth.org)
- This results in secretion of therapeutic levels of ARSB when the universally active cytomegalovirus (CMV) but not the muscle-specific muscle creatine kinase (MCK) promoter is used, suggesting that transduction of extramuscular sites rather than enzyme secretion from muscle occurs after muscle ARSB gene transfer. (elsevier.com)
- Passive and active DOMS, isokinetic muscle performance, creatine kinase (CK), myoglobin and interleukin-6 were assessed immediately before and 24, 48 and 72 h after EIMD. (northumbria.ac.uk)
- The events occurring during phorbol ester mediated destruction of myofibrils in differentiated muscle cells were followed at the fluorescence and electron microscope levels using antibodies which bind troponin-T, a newly discovered 185 000 dalton M-line protein called myomesin and muscle type creatine kinase. (elsevier.com)
- Creatine Kinase (CK) is released from the muscles whenever there is muscle damage. (esamievalori.com)
- Creatine kinase is an enzyme produced by various tissues that catalyzes the conversion of creatine in muscle activity. (kidneypedia.net)
- There are two main recognized types of creatine kinase, type B and type M. The B and M stand respectively for "brain" and "muscle. (kidneypedia.net)
- Normal creatine kinase levels vary by age, gender, muscle mass, and level of activity. (kidneypedia.net)
- Other possible causes of high serum creatine kinase include muscular dystrophy, severe muscle breakdown, and several other rarer causes. (kidneypedia.net)
- The patient's serum creatine kinase activity was 310 000 U/L (reference interval, 30-200 U/L), and his serum myoglobin result was 68 860 [micro]g/L (reference interval, 0-110 [micro]g/L). Damaged muscle cells release creatine kinase (MM isoform), myoglobin, and aspartate aminotransferase, as well as lesser amounts of alanine aminotransferase and lactate dehydrogenase. (thefreelibrary.com)
- Depending on the tissue, different forms of CK are present: CK-MM is primarily present in skeletal muscles, CK-MB represents 30% of CK from the heart, while CK-BB comes from the brain and smooth muscle, such as the intestinal walls. (biron.com)
- Creatine kinase is found in muscle tissue and CK-MM enters the blood stream in increased amounts when there is muscle damage. (worldduchenne.org)
- Results showing elevated CK-MM must be confirmed using other testing methods, such as muscle biopsies, genetic and other laboratory tests. (worldduchenne.org)
- Here, we report an inverse relationship between the activity and acetylation of CK muscle form (CKM) in human and mouse failing hearts. (jci.org)
- Chicken heart muscle contains almost exclusively the BB isoenzyme of creatine kinase (CK), its myofibrils, moreover, lack an M-line. (rupress.org)
- The enzyme is dimeric and exists in three isoenzyme forms: MM (muscle), MB (heart) and BB (brain). (creative-enzymes.com)
- We therefore hypothesised that resveratrol treatment would improve muscle cell differentiation and myotube hypertrophy in differentiating C2C12 myoblasts and mature myotubes during GR. Glucose restriction at 0.6 g/L (3.3 mM) blocked differentiation and myotube hypertrophy versus high-glucose (4.5 g/L or 25 mM) differentiation media (DM) conditions universally used for myoblast culture. (springer.com)
- SUMO1 transfection further attenuated HDAC1-induced inhibition of muscle creatine kinase luciferase activity that was reversed in HDAC1 2R. (bvsalud.org)
- CK-MM and CK-MB are distributed primarily in the skeletel muscle and heart muscle, respectively, while CK-BB is present mainly in the brain and in tissues composed of smooth muscle. (pointescientific.com)
- The exercise protocol promoted slight muscle damage, reflected in significant increases in total creatine kinase in all subjects after exercise. (biomedcentral.com)
- Though CK-MM is the most abundant CK isoenzyme in the cardiac muscles, CK-MB constitutes about 20% of the total CK in the cardiac muscle tissue. (avant-medical.com)
- Direct inhibition of the Na + /Ca 2+ exchanger using amiloride (1 mM) had no effect on muscle 45 calcium uptake or CK efflux where active Na + extrusion via Na + /K + exchange had been prevented. (sruc.ac.uk)
- CK-MB is the isoenzyme of Creatine Kinase most involved in the metabolism of cardiac muscle tissue. (custom-monoclonalantibody.com)
- The muscle isoenzyme (MM-CK) was the predominant form in plasma (99 percent) and its activity increased in response to an episode of acute heat stress. (sruc.ac.uk)
CKMM2
- Recognizes Creatine Kinase MM Isoenzyme (CKMM). (lsbio.com)
- A traditional immunoassay that could distinguish CKMB from CKBB and CKMM was a little longer in coming due to the fact that CKMB has no unique subunits compared to these other forms. (aaltoscientific.com)
Phosphokinase6
- Creatine kinase ( CK ), also known as creatine phosphokinase ( CPK ) or phosphocreatine kinase , is an enzyme ( EC 2.7.3.2 ) expressed by various tissues and cell types. (wikipedia.org)
- All cases were subjected to complete clinical evaluation pedigree analysis, electromyography studies, estimation of serum creatine phosphokinase enzyme (CPK) levels and DNA analysis. (cdc.gov)
- It is also known as CPK (Creatine phosphokinase) MB test. (1mg.com)
- Creatine kinase is also sometimes referred to as creatine phosphokinase, creatine phosphotransferase, CPK, or just CK. (aaltoscientific.com)
- 1. Narayanaswami, A. Creatine Phosphokinase in Mammalian Brain. (aaltoscientific.com)
- Creatine phosphokinase (CPK) is widely distributed and is primarily involved with ATP regeneration in conjunction with the contractile and transport systems. (creative-enzymes.com)
Fingerprint1
- Together they form a unique fingerprint. (elsevier.com)
Antibody5
- The following product was used in this experiment: Creatine Kinase MM Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-86374, RRID AB_2803156. (thermofisher.com)
- Creatine Kinase MM antibody LS-C14686 is an unconjugated mouse monoclonal antibody to human Creatine Kinase MM (CKM). (lsbio.com)
- A high affinity purification column was use to purify Creatine Kinase MM (CK-MM) Goat anti- Polyclonal Antibody by Lee Biosolution by chromatographic size exclusion.Lee Biosolution supplies antibodies that are for research of human proteins. (bioxys.com)
- Includes C-Reactive Protein (CRP), Rheumatoid Arthritis (RA) Factor, Antinuclear Antibody(ANA), Sedimentation Rate, Uric Acid plus Wellness #2 Essential Panel that includes Complete Blood Count (CBC), Comprehensive Metabolic Panel (CMP-14), Lipid Panel with Total Cholesterol: HDL Ratio, Thyroid Panel plus TSH plus Cyclic Citrullinated Peptide (CCP) Antibodies, Creatine Kinase (CPK), Aldolase and Prostate-specific Antigen. (walkinlab.com)
- This antibody completely inhibits the activity of CK-MM and half of the activity of CK-MB, while not affecting the B subunit activity of CK-MB and CK-BB. (pointescientific.com)
Serum17
- In results, decreased creatine kinase activity in brain regions and its increased level in serum observed in CRF induced animals. (omicsonline.org)
- Elevated serum values were found for alanine aminotransferase (407 IU/L), aspartate aminotransferase (639 IU/L), gamma-glutamyltranspeptidase (120 IU/L), lactate dehydrogenase (478 IU/L), creatine kinase (407 IU/L) and aldolase (16,9 IU/L). Urinalysis and clotting studies were normal. (biomedcentral.com)
- However the worth of succinylcholine is limited by the frequent occurrence of muscular side effects which manifest biochemically in the form of rise in serum creatine kinase [CK]. (bvsalud.org)
- Blood samples were drawn for estimation of serum creatinine kinase. (bvsalud.org)
- 3. Smith, A. Diagnostic Value of Serum Creatine Kinase in a Coronary-Care Unit. (aaltoscientific.com)
- Serum Myocardial Creatine Kinase (CK-MB) After Coronary Arterial Bypass Surgery. (aaltoscientific.com)
- Interestingly, other forms of cancer have also resulted in clinically significant levels of elevated CKBB in serum. (aaltoscientific.com)
- Increased Serum Creatine Kinase BB and Neuron Specific Enolase Following Head Injury Indicates Brain Damage. (aaltoscientific.com)
- Serum Creatine Kinase Isoenzyme BB as an indicator of Active Metastatic Disease. (aaltoscientific.com)
- The Diagnostic and Prognostic Value of Pretreatment Serum Creatine Kinase BB Levels in Patients with Neroblastoma. (aaltoscientific.com)
- Clinical Applications of Microarray Technology: Creatine Kinase B is an Up-regulated Gene in Epithelial Ovarian Cancer and Shows Promise as a Serum Marker. (aaltoscientific.com)
- The normal range of serum creatine kinase is between 10 and 120 micrograms per liter of blood. (kidneypedia.net)
- When serum creatine kinase is elevated above the normal range, this can indicate any of several conditions. (kidneypedia.net)
- Vitro CPK (MB) reagent is an immunoinhibition assay intended for the in vitro quantitative determination of Creatine Kinase (CK-MB) in human serum and plasma. (vitroscient.com)
- In the absence of disease, most CKactivity in serum is due to the CK-MM isoform. (vitroscient.com)
- The CK-MB Reagent is for the quantitative determination of the isoenzyme Creatine Kinase-MB (CK-MB) in serum on the Tokyo Boeki Medisys Inc. Biolis 24i analyzer. (pointescientific.com)
- For the quantitative in vitro determination of Creatine Kinase in serum or plasma. (randoxonlinestore.com)
Dimeric1
- Creatine kinase (ATP: Creatine N-phosphotransferase, EC2.7.3.2) is a dimeric enzymecomposed of two types of monomer subunits, M (Muscular) and B (Brain). (vitroscient.com)
Type of creatine kinase2
- The type of creatine kinase located in the brain is called CK-BB. (reference.com)
- these are formed from the basic B and M type of creatine kinase. (kidneypedia.net)
Phosphate7
- Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). (abcam.com)
- One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C. (leebio.com)
- The mitochondrial creatine kinase (CK m ), which produces ATP from ADP by converting creatine phosphate to creatine, is present between the two membranes of the mitochondrion. (wikidoc.org)
- The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine and between phospho-creatine and ADP. (wikipedia.org)
- Creatine kinase catalyzes the reversible transfer of the gamma phosphate from ATP to creatine forming the high energy compound creatine phosphate. (utmb.edu)
- Whereas many of the identified proteins have been previously implicated in schizophrenia, such as fructose-bisphosphate aldolase C, creatine kinase and neuron-specific enolase, new putative disease markers were also identified such as dihydrolipoyl dehydrogenase, tropomyosin 3, breast cancer metastasis-suppressor 1, heterogeneous nuclear ribonucleoproteins C1/C2 and phosphate carrier protein, mitochondrial precursor. (genes2cognition.org)
- The determination of CK using creatine phosphate and adenosine-5'-diphosphate (ADP) as substrates rather than creatine and adenosine-5'-triphosphate (ATP) has several advantages in test performance as it allows for a faster reaction rate resulting in greater sensitivity. (randoxonlinestore.com)
Cardiac3
- Despite experimental and clinical HF data showing reduced metabolism through cardiac creatine kinase (CK), the major myocardial energy reserve and temporal ATP buffer, a causal relationship between reduced ATP-CK metabolism and contractile dysfunction in HF has never been demonstrated. (nih.gov)
- Signs and symptoms that typically accompany an elevated creatine kinase include cardiac symptoms such as chest pain, breathing trouble, dizziness, excessive sweating and pain that reaches the jaw or the lower arm, according to Healthgrades. (reference.com)
- Creatine kinase is physically associated with the cardiac ATP-sensitive K+ channel in vivo. (abcam.cn)
Isoenzyme of creatine3
- Recent results from studies of experimental animals suggest that prompt detection of reperfusion is possible based on analysis of sequential changes in the relative activities of individual isoforms of the MM isoenzyme of creatine kinase (CK) in plasma. (elsevier.com)
- CK MB is a form of the enzyme which means it is an isoenzyme of Creatine kinase (CK). (1mg.com)
- The MB Isoenzyme of Creatine Kinase as an Indicator of Severity of Myocardial Ischaemia. (aaltoscientific.com)
Enzyme creatine kinase3
- The heart, brain and skeletal muscles are the parts of the body that primarily contain the enzyme creatine kinase, states Healthgrades. (reference.com)
- This test measures the enzyme Creatine Kinase (CK) MB in blood. (1mg.com)
- Marked changes in the plasma activities of the intracellular enzyme creatine kinase (CK) have been observed in broiler chickens subjected to a range of stressful stimuli. (sruc.ac.uk)
Higher creatine kinase1
- Athletes and others who engage in strenuous physical activity have higher creatine kinase without it being a sign of anything necessarily wrong with them. (kidneypedia.net)
Subunits5
- Creatine Kinase consists of the M and B subunits and is found in the MM, MB and BB isomorphs. (mediconsa.com)
- Hybridization studies demonstrate the Torpedo electric organ creatine kinase to be composed of identical subunits and capable of producing an enzymatically active heterodimer when combined with canine BB creatine kinase. (mysciencework.com)
- The acetylcholine receptor-associated creatine kinase is, therefore, identical with the cytoplasmic form from the electric organ and is composed of M-subunits. (mysciencework.com)
- The subunits combine to form three distinct CK. (vitroscient.com)
- These subunits associate to form three isoenzymic forms: CK-BB, CK-MM and CK-MB. (avant-medical.com)
Proteins5
- but none of the proteins forming the pre-replication complex (e.g. (biologists.org)
- The GSP Neonatal Creatine Kinase-MM kit works by measuring the concentration of a type of protein called CK-MM, which is part of a group of proteins called creatine kinase. (worldduchenne.org)
- Novel insight into the molecular pathogenesis of EGF induced liver cancer was obtained and among the 37 newly identified proteins several are likely candidates for the development of molecularly targeted therapies and include the nucleoside diphosphate kinase A, bifunctional ATP-dependent dihydroyacetone kinase and phosphatidylethanolamine-binding protein1, the latter being an inhibitor of the Raf-1 kinase. (biomedcentral.com)
- An in vitro kinase assay was performed to assess the phosphorylation activities of a novel CaMK-like protein in T. gondii by using purified proteins with various concentrations of calcium, calmodulin antagonists, or T. gondii glideosome proteins. (biomedcentral.com)
- Indirect immunofluorescence microscopy was performed to detect the localization of this protein kinase by using the antibodies against this protein and organellar maker proteins of T. gondii . (biomedcentral.com)
Abstract2
- Sahin, N;Hayirli, A;Orhan, C;Tuzcu, M;Komorowski, J R;Sahin, K 2018-04-01 00:00:00 Abstract This experiment was conducted to compare the effect of the supplemental chromium (Cr) form on performance, egg quality, and metabolic profile in laying hens exposed to heat stress (HS). (deepdyve.com)
- Although reports can be found in abstract form, both supporting (5) and refuting (6-8) the unblemished sensitivity of diffusion-weighted imaging for hyperacute ischemic stroke, false-negative findings in the radiologic literature have generally consisted of resolving deficits (9-11) or radiographically unconfirmed strokes (10, 11) . (ajnr.org)
Cytosolic1
- While mitochondrial creatine kinase is directly involved in formation of phospho-creatine from mitochondrial ATP, cytosolic CK regenerates ATP from ADP, using PCr. (wikipedia.org)
Protein kinase1
- We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. (biomedcentral.com)
Gene3
- There is a third gene product which results in the mitochondrial form of CK. (aaltoscientific.com)
- CKM (Creatine Kinase, M-Type) is a Protein Coding gene. (genecards.org)
- GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and creatine kinase activity . (genecards.org)
Adenosine1
- CK catalyses the conversion of creatine and utilizes adenosine triphosphate (ATP) to create phosphocreatine (PCr) and adenosine diphosphate (ADP). (wikipedia.org)
Tissues3
- [2] Thus creatine kinase is an important enzyme in such tissues. (wikipedia.org)
- No creatine kinase isoenzyme with an electrophoretic mobility similar to mammalian BB creatine kinase was seen in any of the Torpedo tissues examined. (mysciencework.com)
- CK (creatine kinase) is an enzyme (protein) found in several tissues, including muscles and the heart. (biron.com)
Differentiating patients1
- Accordingly, assessment of activities of MM CK isoforms in plasma should prove useful in differentiating patients with and without reperfusion at a time when additional interventions needed to consolidate the gains accompanied by initial pharmacological treatment can still be initiated effectively. (elsevier.com)
Brain5
- Crystal structure of human brain-type creatine kinase with ADP and creatine. (wikipedia.org)
- Increased creatine levels often accompany heart attacks, trauma, stroke, brain injury and conditions that impair the brain or skeletal muscles. (reference.com)
- While CK MM is found in skeletal muscles and heart, CK BB is found in the brain and smooth muscles. (1mg.com)
- In the brain the BB form predominates. (aaltoscientific.com)
- Brain Creatine Kinase in Blood After Acute Brain Injury. (aaltoscientific.com)
Myocardial2
Heart and skeletal muscles1
- CK-MM is a form of creatine kinase found in the heart and skeletal muscles, whereas CK-MB is present in the heart. (reference.com)
Phosphorylation2
- KCC2 and NKCC1 functions are also regulated by phosphorylation by enzymes such as PKC, Src-family tyrosine kinases, and WNK1-4 and their downstream effectors STE20/SPS1-related proline/alanine-rich kinase (SPAK)-oxidative stress responsive kinase-1 (OSR1). (frontiersin.org)
- We identified a novel CaMK homolog in T. gondii , T. gondii CaMK-related kinase (TgCaMKrk), which exhibits calmodulin-independent autophosphorylation and substrate phosphorylation activity. (biomedcentral.com)
Isoform1
- In 11 of the 12 patients without reperfusion, the percentage in plasma of the MM CK isoform released from the heart, MM, did not change or decreased in the first hour. (elsevier.com)
Activity4
- Due to the important physiological role in excitable cells, the activity and level of creatine kinase (CK) play a crucial role in maintaining body functions. (elsevier.com)
- Specimens were analyzed for total CK, CK-MM, CK-MB, and CK-BB activity. (utmb.edu)
- Hyperacetylation of recombinant CKM disrupted MM homodimer formation and reduced enzymatic activity, which could be reversed by sirtuin 2 treatment. (jci.org)
- However, calmodulin antagonists had no effect on its kinase activity. (biomedcentral.com)
Determination1
- Determination of Creatine Kinase Isoenzyme MB (CK-MB): Comparison of Methods and Clinical Evaluation. (aaltoscientific.com)
Muscles3
- MMM is an inflammatory condition which specifically affects the muscles of mastication or chewing. (petplace.com)
- The production of creatine occurs primarily in the kidneys and the liver, but 95% of it is found in the skeletal muscles. (kidneypedia.net)
- ESR was 82 mm in the first hour and CK was 58 U/L. Ultrasound examination revealed diffuse enlargement of muscles with preserved intermuscular fat planes in the anterior aspect of right thigh. (neurologyindia.com)
Recombinant1
- Recombinant fragment corresponding to Pig Creatine Kinase MM aa 11-367. (abcam.com)
Acute5
- The disease occurs in both acute (sudden) and chronic forms, and the signs may vary with each form. (petplace.com)
- Dogs with the acute form of the disease may be lethargic, have a fever, react painfully to touching the head, experience excessive salivation and be reluctant to chew or eat. (petplace.com)
- Creatine Kinase Isoenzyme MB (CK-MB) in Acute Coronary Ischemia. (aaltoscientific.com)
- High creatine kinase can also be a sign of acute renal failure. (kidneypedia.net)
- Because of the illnesses it can indicate, a test for creatine kinase is indicated whenever a patient suffers from symptoms of heart attack, acute renal failure, or muscular breakdown. (kidneypedia.net)
HUMAN7
- Synthetic peptide, corresponding to the N-terminus of Human Creatine Kinase M. (thermofisher.com)
- Lee Biosolutions is the leading manufacturer of human Creatine Kinase MM (CK-MM) isoenzyme for medical research and diagnostic manufacturing. (leebio.com)
- This includes physical injury 3,4,5 , various types of cancer 6,7 and neurodegenerative diseases such as Alzheimer's disease 8 , CJD 9 (the human form of cow disease) and a host of others 10 . (aaltoscientific.com)
- NH2-terminal amino acid sequence determinations show considerable sequence homology between human MM, Torpedo electric organ, chicken MM, and porcine MM creatine kinase. (mysciencework.com)
- Creatine is a substance that is naturally produced by the human body from amino acids. (kidneypedia.net)
- CK1 recognises human creatine kinase MB isoenzyme. (abcam.cn)
- hCK-MB - 100% hCK-BB - 182% hCK-MM - 7.3% Affinity constant: 2x10 9 l/mol human CK-MB. (abcam.cn)
Diagnostic1
- In clinical diagnostic testing, creatine kinase levels in the blood are measured as part diagnosis of a number of illnesses. (kidneypedia.net)
Significance1
- In Xenopus , RPA assembles in nuclear foci that form before DNA synthesis, but their significance in the assembly of replication initiation complexes has been questioned. (biologists.org)
Muscular3
- So far, three main phenotypes have been reported: Miyoshi myopathy (MM), limb girdle muscular dystrophy type 2B (LGMD 2B), and distal myopathy with anterior tibial onset (DMAT). (neurologyindia.com)
- A percentage of CK-MM greater than 95% indicates that the increase in total CK was muscular in origin. (biron.com)
- The GSP Neonatal Creatine Kinase-MM kit is not intended for DMD diagnosis or for screening of other forms of muscular dystrophies. (worldduchenne.org)
Xenopus1
- These data show that in Xenopus , the hypophosphorylated form of RPA34 is a component of the pre-initiation complex. (biologists.org)