A transferase that catalyzes formation of PHOSPHOCREATINE from ATP + CREATINE. The reaction stores ATP energy as phosphocreatine. Three cytoplasmic ISOENZYMES have been identified in human tissues: the MM type from SKELETAL MUSCLE, the MB type from myocardial tissue and the BB type from nervous tissue as well as a mitochondrial isoenzyme. Macro-creatine kinase refers to creatine kinase complexed with other serum proteins.
A form of creatine kinase found in the BRAIN.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
An amino acid that occurs in vertebrate tissues and in urine. In muscle tissue, creatine generally occurs as phosphocreatine. Creatine is excreted as CREATININE in the urine.
An isoenzyme of creatine kinase found in the MUSCLE.
A form of creatine kinase found in the MITOCHONDRIA.
An isoenzyme of creatine kinase found in the CARDIAC MUSCLE.
Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.
Analyses for a specific enzyme activity, or of the level of a specific enzyme that is used to assess health and disease risk, for early detection of disease or disease prediction, diagnosis, and change in disease status.
An endogenous substance found mainly in skeletal muscle of vertebrates. It has been tried in the treatment of cardiac disorders and has been added to cardioplegic solutions. (Reynolds JEF(Ed): Martindale: The Extra Pharmacopoeia (electronic version). Micromedex, Inc, Englewood, CO, 1996)
An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.
A strain of Rattus norvegicus which is a model for spontaneous insulin-dependent diabetes mellitus (DIABETES MELLITUS, INSULIN-DEPENDENT).
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
An enzyme that catalyzes the phosphorylation of the guanidine nitrogen of arginine in the presence of ATP and a divalent cation with formation of phosphorylarginine and ADP. EC 2.7.3.3.
Contractile tissue that produces movement in animals.
Agents that inhibit PROTEIN KINASES.
A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)
A membrane bound member of the TNF superfamily that is expressed on activated B-LYMPHOCYTES; MACROPHAGES; and DENDRITIC CELLS. The ligand is specific for the 4-1BB RECEPTOR and may play a role in inducing the proliferation of activated peripheral blood T-LYMPHOCYTES.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Antibodies produced by a single clone of cells.
An imprecise term which may refer to a sense of spatial disorientation, motion of the environment, or lightheadedness.
Bony structure of the mouth that holds the teeth. It consists of the MANDIBLE and the MAXILLA.
Any of several large carnivorous mammals of the family CANIDAE that usually hunt in packs.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Electrophoresis in which cellulose acetate is the diffusion medium.
Proteins that are chemically bound to a substrate material which renders their location fixed. The immobilization of proteins allows their use in chemical reactions without being diluted by solvent.
Methods for cultivation of cells, usually on a large-scale, in a closed system for the purpose of producing cells or cellular products to harvest.
Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.
Support systems that provide assistance and encouragement to individuals with physical or emotional disabilities in order that they may better cope. Informal social support is usually provided by friends, relatives, or peers, while formal assistance is provided by churches, groups, etc.
It is a form of protection provided by law. In the United States this protection is granted to authors of original works of authorship, including literary, dramatic, musical, artistic, and certain other intellectual works. This protection is available to both published and unpublished works. (from Circular of the United States Copyright Office, 6/30/2008)
A competitive team sport played on a rectangular field. This is the American or Canadian version of the game and also includes the form known as rugby. It does not include non-North American football (= SOCCER).
Injuries incurred during participation in competitive or non-competitive sports.
A surgical specialty concerned with the treatment of diseases and disorders of the brain, spinal cord, and peripheral and sympathetic nervous system.
A medical specialty concerned with the study of the structures, functions, and diseases of the nervous system.
A genus in the family ORTHOMYXOVIRIDAE causing influenza and other diseases in humans and animals. It contains many strains as well as antigenic subtypes of the integral membrane proteins hemagglutinin (HEMAGGLUTININS) and NEURAMINIDASE. The type species is INFLUENZA A VIRUS.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A family of scavenger receptors that are predominately localized to CAVEOLAE of the PLASMA MEMBRANE and bind HIGH DENSITY LIPOPROTEINS.
Proteins prepared by recombinant DNA technology.
The long cylindrical contractile organelles of STRIATED MUSCLE cells composed of ACTIN FILAMENTS; MYOSIN filaments; and other proteins organized in arrays of repeating units called SARCOMERES .
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
The hollow, muscular organ that maintains the circulation of the blood.
The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.
The rate dynamics in chemical or physical systems.

Production of recombinant human creatine kinase (r-hCK) isozymes by tandem repeat expression of M and B genes and characterization of r-hCK-MB. (1/46)

BACKGROUND: Serum creatine kinase-MB isoenzyme (CK-MB) is widely used as a marker of myocardial injury. We prepared recombinant human CK (r-hCK) MB isoenzyme and examined its potential for use as a control material for assay of CK-MB in serum. METHODS: cDNAs encoding CK-M and CK-B subunits were inserted into the same plasmid vector, followed by transformation of Escherichia coli. The resulting three types of CK isoenzymes were purified by conventional chromatography. RESULTS: The ratio of MB to MM to BB was 50:40:10 on the basis of CK activity. Highly purified CK-MB with a specific activity of 533 U/mg was produced in a yield of 5.7 mg/g of packed cells. Purified r-hCK-MB had the isoelectric point (pI 5.3) and molecular size (46 kDa for the subunit) of native CK-MB. Its immunoreactivity in an ELISA using antibody against native heart enzyme was similar to that of cardiac CK-MB. The r-hCK-MB retained >90% activity for at least 4 months at 11 degrees C in a delipidated serum matrix in a liquid form at a concentration of 118 U/L. CONCLUSIONS: r-hCK-MB shows key properties of the native cardiac isoenzyme and may be useful as a control and calibrator for serum assays of CK-MB.  (+info)

Identification of novel markers for monitoring minimal residual disease in acute lymphoblastic leukemia. (2/46)

To identify new markers of minimal residual disease (MRD) in B-lineage acute lymphoblastic leukemia (ALL), gene expression of leukemic cells obtained from 4 patients with newly diagnosed ALL was compared with that of normal CD19(+)CD10(+) B-cell progenitors obtained from 2 healthy donors. By cDNA array analysis, 334 of 4132 genes studied were expressed 1.5- to 5.8-fold higher in leukemic cells relative to both normal samples; 238 of these genes were also overexpressed in the leukemic cell line RS4;11. Nine genes were selected among the 274 overexpressed in at least 2 leukemic samples, and expression of the encoded proteins was measured by flow cytometry. Two proteins (caldesmon and myeloid nuclear differentiation antigen) were only weakly expressed in leukemic cells despite strong hybridization signals in the array. By contrast, 7 proteins (CD58, creatine kinase B, ninjurin1, Ref1, calpastatin, HDJ-2, and annexin VI) were expressed in B-lineage ALL cells at higher levels than in normal CD19(+)CD10(+) B-cell progenitors (P <.05 in all comparisons). CD58 was chosen for further analysis because of its abundant and prevalent overexpression. An anti-CD58 antibody identified residual leukemic cells (0.01% to 1.13%; median, 0.03%) in 9 of 104 bone marrow samples from children with ALL in clinical remission. MRD estimates by CD58 staining correlated well with those of polymerase chain reaction amplification of immunoglobulin genes. These results indicate that studies of gene expression with cDNA arrays can aid the discovery of leukemia markers. (Blood. 2001;97:2115-2120)  (+info)

Comparisons of the effects of tamoxifen, toremifene and raloxifene on enzyme induction and gene expression in the ovariectomised rat uterus. (3/46)

This study compares the actions of oestradiol, tamoxifen, toremifene and raloxifene on enzyme and gene expression in uterine tissues of ovariectomised rats over 72 h. The time-course for the induction of ornithine decarboxylase by the compounds showed a rapid biphasic response, while for creatine kinase brain type (BB) there was a continued increase over 72 h. The efficacy of induction showed that, with both markers, oestradiol gave the highest induction level, followed by tamoxifen or toremifene and then raloxifene. RT-PCR demonstrated that all compounds decreased oestrogen receptor (ER) alpha, ERbeta and ERbeta2 gene expression, 8-24 h after the first dose, suggesting that down-regulation of ER is not the primary cause of the difference in efficacy between these compounds. Using cDNA arrays, expression of 512 genes was examined in the uteri of oestradiol- or tamoxifen-treated rats. Both compounds resulted in the up-regulation of heat-shock protein 27, telomerase-associated protein 1 and secretin. However, most surprising was the marked down-regulation of Wilms' tumour and retinoblastoma genes. We speculate that this may result in a loss of regulation of the transition from the G1 to the S phase in the cell cycle and may make cells more vulnerable to the carcinogenic effects of tamoxifen in this tissue.  (+info)

Effect of weight bearing on recovery from nerve injury in skeletal muscle. (4/46)

We examined the effect of weight bearing (WB) on muscle recovery after nerve injury. Rats were housed in individual cages for 2 wk under WB or hindlimb suspension (HS) after being subjected to sciatic nerve compression for 1 wk. Sham operated on rats served as controls (sham group). We used 31P- and 19F-nuclear magnetic resonance spectroscopy combined with histochemical, physiological, and biochemical techniques to assess the outcome in the three groups. Creatine kinase-BB (CK-BB) mRNA levels expression, CK activity, and type I fiber density in the WB group were elevated compared with those in the HS group. In addition, sciatic functional index, tetanic tension, energy state, and local circulation dynamics of the WB group were greater than those of the HS group. These results suggested that WB plays an important role in muscle regeneration, inhibits the reduction of CK activity, and facilitates the activation of neural recovery, energy state, and local circulation dynamics.  (+info)

Expression of brain subtype creatine kinase in the zebrafish embryo. (5/46)

Creatine kinases (CK) play crucial roles in intracellular energy transfer. We have isolated a cDNA from zebrafish embryos, which encodes a CK highly related to the mammalian brain subtype creatine kinase (BCK). The bck mRNA is expressed maternally in the zebrafish embryo and transcripts are distributed uniformly in blastula and gastrula stages. Expression becomes restricted to the prechordal plate and the nervous system during subsequent somitogenesis stages. bck transcripts are abundant in primary neurons in the developing central nervous system of the 1-day-old embryo. While some bck expression persists in the hindbrain, expression vanishes in the spinal cord of the 2-day-old embryo. In summary, the expression pattern of bck is highly dynamic and suggests a role for bck during gastrulation and neuronal differentiation.  (+info)

Postoperative patterns and kinetics of cTnI, cTnT, CK-MB-activity and CK-activity after elective aortic valve replacement. (6/46)

OBJECTIVES: The aim of this prospective study was to evaluate postoperative kinetics of four different biochemical ischaemic markers after elective aortic valve replacement (AVR). Additionally, pre-, peri- and postoperative data were analysed in order to identify factors with possible impact on the postoperative release of the selected enzymes. DESIGN: Forty patients (14 males, 26 females, aged 70 +/- 11 years; EF = 54 +/- 18% [mean +/- SD]) undergoing elective AVR were prospectively included in this study. For all patients, serum concentrations of cTnI, cTnT, and serum activities of CK-MB and CK were measured preoperatively as well as 0, 6, 12, 24, 48 and 120 hours after removal of the aortic cross-clamp. Clinical data were assessed in all patients and correlated with postoperative enzyme patterns. RESULTS: There were no major complications. Preoperatively, all patients showed enzyme values in the normal range whereas the four ischaemic markers reached higher values postoperatively. cTnI reached its maximum values 24 hours (XMed = 2.35 micrograms/L, 95%-CI [2.0, 3.3]) and cTnT 48 hours after the operation (XMed = 0.239 microgram/L, 95%-CI [0.174, 0.283]). Typical biphasic release kinetics could be demonstrated for cTnT. There was a high linear correlation between cTnI and cTnT at all sampling times. In contrast, a high linear correlation between cTnI, cTnT, and CK-MB-activity was only found 48 hours after aortic unclamping. cTnI nearly was in normal range 120 h postoperatively (XMed = 0.5 microgram/L, 95%-CI [0.2, 0.6]), whereas cTnT still remained pathologically elevated (XMed = 0.223 microgram/L, 95%-CI [0.137, 0.299]). No linear correlation was found between maximum values of the ischaemic markers postoperatively and age, gender, body surface area, ejection fraction, LV-hypertrophy, operating time, ECC time, time of cardiac arrest, lowest body temperature, perfusion pressure, cardioplegia volume, reperfusion time, postoperative septiformic circulatory instability, or ventilation time. CONCLUSIONS: All four ischaemic markers showed individual peak characteristics and kinetics after uncomplicated AVR. In contrast to previous findings, aortic cross-clamping time had no detectable impact on postoperative peak patterns of any ischaemic marker.  (+info)

Two specific markers for neural differentiation of embryonal carcinoma cells. (7/46)

Two multipotential embryonal carcinoma (EC) cell lines, 1003 and 1009, can be induced to form preferentially neural derivatives in vitro. Synthesis of specific proteins during neural differentiation was followed by two-dimensional gel electrophoresis. The comparison of protein patterns obtained with neural and non-neural derivatives of these EC cell lines indicates that two changes are specific for the neural pathway: (i) the appearance of a new beta-tubulin isoform and (ii) the accumulation of the brain isozyme of creatine phosphokinase already present in small amounts in EC stem cells. These changes were found to take place early in the course of differentiation and to occur even when neurite outgrowth was prevented.  (+info)

Expression of creatine kinase isoenzyme genes during postnatal development of rat brain cerebellum: evidence for transcriptional regulation. (8/46)

Transcription and accumulation of brain-type creatine kinase (CKB) mRNA and its protein was examined during postnatal development of rat brain cerebellum, the brain region containing highest CKB mRNA in the adult. CKB protein was extremely low at day 1, increased about 10-fold until week 4 and remained constant until week 10. This time course was paralleled by cerebellar CKB mRNA, which was also extremely low at day 1 and increased 5-fold during the first 3 weeks and then remained constant. High levels of CKB protein were also detected in cultured primary cerebellar granular neurons. Nuclear run-on assays directly showed that CKB mRNA accumulation during postnatal cerebellar development was due to increased transcription. When compared with cerebrum and whole brain, cerebellar CKB mRNA accumulation during postnatal development was temporally delayed. Analysis of myocyte enhancer factor (MEF)-2 and Sp1, factors known to initiate or sustain CKB transcription in tissues other than brain, revealed that MEF-2 in cerebellum was low at week 1 but increased 3.5-fold by week 7, while Sp1 remained unchanged. The increase in CKB protein during cerebellar postnatal development was coincident with that of the ubiquitous mitochondrial CK protein and mRNA, indicating that a functional phosphocreatine energy shuttle probably exists for efficient ATP regeneration in the cerebellum. This should be beneficial for the many energy-demanding requirements during cerebellar development, as indicated by the observed temporal co-expression of CKB with myelin basic protein, which is involved in axon myelination by oligodendrocytes.  (+info)

Purification and characteriization of the heterogeneous brain-type creatine kinase in chicken, study of the functional aspects of this heterogeneity in different ...
Phagocytosis requires locally coordinated cytoskeletal rearrangements driven by actin polymerization and myosin motor activity. How this actomyosin dynamics is dependent upon systems that provide access to ATP at phagosome microdomains has not been determined. We analyzed the role of brain-type creatine kinase (CK-B), an enzyme involved in high-energy phosphoryl transfer. We demonstrate that endogenous creatine kinase (CK-B), in macrophages is mobilized from the cytosolic pool and coaccumulates with F-actin at nascent phagosomes. Live cell imaging with XFP-tagged CK-B and β-actin revealed the transient and specific nature of this partitioning process. Overexpression of a catalytic dead CK-B or CK-specific cyclocreatine inhibition caused a significant reduction of actin accumulation in the phagocytic cup area, and reduced complement receptor-mediated, but not Fc-γR-mediated, ingestion capacity of macrophages. Finally, we found that inhibition of CK-B affected phagocytosis already at the stage ...
RPC030Mu01, Recombinant Creatine Kinase B (CK-BB), CKB; CKBB; CK-B; CK-1; Creatine Kinase, Brain | Products for research use only!
Persistent infection with hepatitis C pathogen (HCV) is a significant reason behind chronic liver organ diseases. creatine kinase B (CKB) an integral ATP-generating enzyme that regulates ATP in subcellular compartments of nonmuscle cells can be important for effective replication from the HCV genome and propagation of infectious pathogen. CKB interacts with HCV NS4A forms and proteins a organic with NS3-4A which possesses multiple enzyme actions. CKB upregulates both NS3-4A-mediated unwinding of DNA and RNA in vitro and replicase activity in permeabilized HCV replicating cells. Our outcomes support a model where recruitment of CKB towards the HCV RC area which includes high and fluctuating energy needs through its discussion with NS4A can be important for effective replication from the viral genome. The CKB-NS4A association can be a potential focus on for the introduction of a new kind of LX 1606 antiviral restorative technique. Hepatitis C pathogen (HCV) disease represents a substantial LX 1606 ...
Rabbit recombinant monoclonal Creatine kinase B type antibody [EPR3926] validated for WB, IP, IHC, ICC, Flow Cyt and tested in Human, Mouse and Rat. Referenced…
ALDOC Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 364 amino acids (1-364 a.a.).
Because p97/CDC‐48 is involved in protein degradation [5], we reasoned that it might modulate ER‐stress‐induced ckb‐2p transcription by eliminating a transcriptional repressor. To address this hypothesis, we designed an RNAi suppressor screen to identify genes whose knockdown could restore tunicamycin ckb‐2p::gfp activation following ER stress in a cdc‐48.2(−/−) mutant background ([4]; Fig 1C). We performed the screen in liquid culture by feeding cdc‐48.2(−/−); ckb‐2p::gfp synchronized L1 larvae with double‐stranded RNA (dsRNA)‐expressing E. coli derived from the C. elegans ORFeome library that targets 11,698 open reading frames covering 62% of C. elegans genes [6]). We then exposed the worms to a concentration of tunicamycin (0.5 μg/ml for 16 h) leading to maximal ckb‐2p::gfp induction in WT worms grown in liquid culture and analyzed them by flow cytometry [7] to measure their length, number and fluorescence intensity. Each RNAi clone was tested in duplicate, and ...
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How is Creatine Kinase B-Subunit Activity abbreviated? CK-B stands for Creatine Kinase B-Subunit Activity. CK-B is defined as Creatine Kinase B-Subunit Activity very rarely.
Anti-Sarcomeric Alpha Actinin antibody [EA-53] (ab9465) has been cited in 63 publications. References for Human, Mouse, Rat, Goat, Pig, Xl, Xp in Flow Cyt…
引用Abcams Anti-Sarcomeric Alpha Actinin抗体[EP2529Y] (ab68167)的参考文献列表。为您列举引用本产品的发表文章,并提供信息包括论文文献数据库中的检索编号以便您搜寻文章
TY - JOUR. T1 - Creatine kinase-catalyzed reaction rate in the cyanide-poisoned mouse brain. AU - Holtzman, David. AU - Offutt, Martin. AU - Tsuji, Miles. AU - Neuringer, Leo J.. AU - Jacobs, Danny. N1 - Copyright: Copyright 2017 Elsevier B.V., All rights reserved.. PY - 1993. Y1 - 1993. N2 - Brain creatine kinase (CK)-catalyzed phosphorus flux from phosphocreatine (PC) to ATP was measured in vivo in young adult mice made reversibly hypoxic by injection of cyanide. Phosphorus spectra and saturation transfer measurements of CK-catalyzed flux were acquired using a high-field (8.45 T) nuclear magnetic resonance (NMR) spectrometer. After low cyanide doses (1-3 mg/kg of body weight), there were no measurable changes in brain pH or in concentrations of PC, the nucleoside triphosphates (including ATP), and Pi. The CK-catalyzed phosphorus flux increased about 75% after the low cyanide dose. Higher doses (4-6 mg/kg) produced a transient 30-40% decrease in PC concentration, doubling of Pi, and a 0.2 unit ...
Established in 1979 from the bone marrow aspirated from a 55-year-old white man with small cell lung carcinoma prior to treatment; corresponds to NCI-H209; described as expressing neuroendocrine biochemical markers, e.g. neuron-specific enolase, brain creatine kinase, L-DOPA decarboxylase and ...
The carrier status of the HMSN/ACC gene can be confirmed through genetic testing. If you have a family history of HMSN/ACC, you may wish to consult a genetic counselor to understand your chances of having a child affected with HMSN/ACC and discuss family planning alternatives.. A paediatrician and/or neurologist will conduct a clinical examination, as well as genetic testing to confirm diagnosis of HMSN/ACC. Additional testing may be recommended, such as a brain scan performed with highly precise radiography technology (cerebral tomography) or an analysis of the electrical activity in the arm and leg muscles (electromyogram).. Symptoms appear shortly after birth or during the first year of life and are characterized by a lack of muscle strength and a delay in the development of motor skills. HMSN/ACC results in a loss of mobility and developmental abnormalities in the spine, hands, and feet. In addition to the physical symptoms, HMSN/ACC also causes mild to moderate cognitive impairment.. Babies ...
Mouse Monoclonal Anti-Alpha Actinin 4 Antibody (4D10) [DyLight 488]. Validated: WB, ELISA, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat. 100% Guaranteed.
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Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
On sale today. 25% Off nostalgic warehouse ckb_occ oval clear crystal 1-1/8 inch mushroom cabinet knob satin nickel is going fast.
Clearly my myosin LC2 and my alpha actinin genes count as two (or is it 4, because I have two copies of each), but do we have 2 more if we add in those of the mouse? And how about the chimpanzee, if we discover that (unlike the mouse) their genes/alleles are absolutely identical to mine? Do we have 2 more when we add in the alleles of the mayor of my town (which, unbeknowst to her have synonomous substitions that make the protein-encoding portion of her DNA, but not the protein itself, different from mine)? Or how about those of the guy next door, who has a neutral mutation in an intron region? Or my third cousin, who has a neutral AA substition in his proteins ...
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UniProtKB IDs are not shown, 1) if protein (or gene) isotypes are not specified in the referred articles, 2) if their IDs are not found in the UniprotKB database or by us ...
chains in the Genus database with same CATH superfamily 4KYO B; 1QQE A; 2IFU A; 2L6J A; 4HOQ A; 5ARG A; 4AM9 A; 3MA5 A; 2XGO A; 5DIZ A; 5BW8 C; 2P58 C; 3LKU A; 5BT1 A; 3LY8 A; 3U4T A; 2J9Q A; 4UQX A; 2VQ2 A; 3Q47 B; 3CK7 A; 1NZN A; 4G2V A; 4I9E A; 5D0Q D; 2HYZ A; 4I2W A; 1A17 A; 1QZ2 A; 4W9R A; 3CK8 A; 4G23 A; 2E2E A; 3GZ2 A; 2UWJ G; 2KCV A; 3MV2 B; 5AYW D; 1WM5 A; 1KT1 A; 3CKB A; 3MV3 B; 4EQF A; 5CCL A; 2FI7 A; 3AS4 A; 5D0O D; 3RJV A; 5M72 A; 4YVQ C; 3LY9 A; 3RKV A; 2Y4U A; 4UI9 K; 4H7X A; 4JA9 A; 3CKC A; 5HI7 A; 3PE4 A; 5FT9 A; 2HR2 A; 5FZS A; 3JYS A; 4B94 A; 5KJL A; 1PC2 A; 2Y4T A; 2NC9 A; 4JL0 A; 4ME2 A; 4U04 A; 3FFL A; 3LCA A; 4JHR A; 4N3C A; 3ZGQ A; 3ASD A; 4ABN A; 1KT0 A; 1ZBP A; 3N71 A; 4YVO A; 4BWR A; 2XPI A; 4HOT A; 4GCO A; 4UQY A; 2GW1 A; 2YHC A; 3MEK A; 4N3B A; 2KAT A; 4I17 A; 2UY1 A; 4Y6C A; 5A7D B; 2HO1 A; 4N39 A; 4F3V A; 3EDT B; 2AVP A; 4HOR A; 5EX3 A; 3R9A B; 4WNG A; 4LEU A; 3RIB A; 3E4B A; 2VSN A; 2UY1 B; 1P5Q A; 5BNW A; 2FEZ A; 2QFC A; 4XIF A; 3UQ3 A; 3OXL A; 1NA0 A; 5JJ6 A; ...
tomografía axial computarizada (es); CAT (is); Imbasan tomografi berkomputer (ms); Компютърна томография (bg); Bilgisayarlı tomografi (tr); Počítačová tomografia (sk); компютерна томографія (uk); 엑스선 전산화 단층 촬영 (ko); Komputila tomografio (eo); počítačová tomografie (cs); Kompjuterizirana tomografija (bs); tomodensitométrie (fr); računalna tomografija (hr); CT (vi); datortomogrāfija (lv); компјутеризована томографија (sr); tomografia computadorizada (pt-br); x-ray computit tomografie (sco); Computertomographie (lb); komputertomografi (nn); computertomografi (nb); Kompyuter tomoqrafiyası (az); ಗಣಕೀಕೃತ ಛೇದಚಿತ್ರಣ (kn); سی تی سکەن (ckb); computed tomography (en); تصوير مقطعي محوسب (ar); Tomografiez jedet (br); 電腦斷層掃描 (yue); komputertomográfia (hu); Thien-nó Thôn-chhèn Só-mèu (hak); tomografia computada (ca); Computertomographie ...
dolor (es); Ɛyaw (ak); Sársauki (is); sakit (ms); Болка (bg); durere (ro); Bolesť (sk); біль (uk); Doloro (io); 고통 (ko); বিষ (as); doloro (eo); bolest (cs); bol (bs); ব্যথা (bn); douleur (fr); bol (hr); ווייטאג (yi); वेदना (mr); đau (vi); sāpes (lv); Skausmos (sgs); бол (sr); Kurwadziwa (sn); Thiàⁿ (nan); smerte (nb); Ağrı (az); ನೋವು (kn); ئازار (ckb); pain (en); الألم (ar); Poan (br); боль (be-tarask); 痛 (yue); Fájdalom (hu); ህመም (am); poovčâs (smn); Min (eu); smärta (sv); Dolor (ast); Wehdaag (nds); Nanay (qu); poen (cy); Ауырсыну (kk); боль (be); درد (fa); 疼痛 (zh); smerte (da); दुखाई (ne); 疼痛 (ja); Dhimbja (sq); боль (ru); الم (arz); bol (sh); כאב (he); Dolor (la); Ul-ol (war); दर्द (hi); 痛 (wuu); bávččas (se); Ցաւ (hyw); Schmerz (de); dolor (oc); வலி (ta); dolore (it); ਪੀੜ (pa); ความเจ็บปวด (th); Doulè (ht); ...
Recombinant protein from the full-length sequence of homo sapiens actinin alpha 1 (ACTN1), transcript variant 2 (NM_001102), with a His tag., from EUPROTEIN
Gentaur molecular products has all kinds of products like :search , Prospecbio \ Recombinant Human Creatine Kinase Brain, His Tag CKB His \ cki-274 for more molecular products just contact us
Actinin alpha (pan), 0.1 mg. The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins.
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BACKGROUND AND PURPOSE: Brain creatine (Cr) deficiencies (BCr-d) are rare disorders of creatine biosynthesis and transport. We performed consecutive measures of total Cr (tCr) and of its phosphorylated fraction, phosphocreatine (PCr), in the brains of children affected by Cr synthesis defects during a long period of therapy. The aim was to identify the optimal treatment strategy for these disorders. MATERIALS AND METHODS: Two patients with guanidinoacetate methyltransferase defect (GAMT-d) were treated with different amounts of Cr and with diet restrictions aimed at reducing endogenous guanidinoacetate (GAA) synthesis. Three patients with arginine:glycine amidinotransferase defect (AGAT-d) were treated with different Cr intakes. The patients treatments were monitored by means of (1)H- and (31)P-MR spectroscopy. RESULTS: Cr and PCr replenishment was lower in GAMT-d than in AGAT-d even when GAMT-d therapy was carried out with a very high Cr intake. Cr and especially PCr replenishment became more ...
Shop a large selection of All Primary Antibodies products and learn more about Creatine Kinase BB, Mouse anti-Human, DyLight 350, Clone: CKB/1268, Novus Biologicals. 0.1 mL;
Shop a large selection of products and learn more about Creatine Kinase BB, Mouse anti-Human, DyLight 488, Clone: CKB/1268, Novus Biologicals. 0.1 mL; DyLight 488.
REP Prep (Cat. No. 3100), QuickGel Dispo Cup Tray (Cat. No. 3353), SPIFE QuickGel Electrodes (Cat. No. 1111), SPIFE QuickGel Gel Holder (Cat. No. 3358), CK/LD Control (Cat. No. 5134), QuickGel Gel Block Remover (Cat. No. 1262), SPIFE 3000/REP 3 Reagent Spreaders (Cat. No. 3706), SPIFE Dispo Sample Cups - Deep Well (Cat. No. 3360), Chamber Cover (Part No. 8JP34012), SPIFE QuickGel Chamber Alignment Guide (886541003), and SPIFE Reagent Spreader (Cat. No. 3386).. ...
Our results suggest that the clinical differences between mania and depression states are supported by contrasting levels of creatine kinase CK . The lack of correlations between creatine kinase level and motor items suggest that creatine kinase CK level in mania versus depression could emphasize the thinking speed and not the motor one ...
Background: Biomarkers specificity is an important factor for their reliable utilization. Known markers for acute myocardial infarction (AMI) including creati...
Bovine Creatine kinase ELISA Kit product information; Bovine Creatine kinase ELISA Kit is available 5 times from supplier abebio at Gentaur.com shop
sp:CHKB_MOUSE] Chkb, Chetk, Chkl, Ck, Ck/Ek, Ck/Ek-beta, Ckb, Ckekb, Ek; choline kinase beta; K14156 choline/ethanolamine kinase [EC:2.7.1.32 2.7.1.82] ...
AMPK and CKB cooperate under energy stress to compensate for loss of adenosine triphosphate (ATP) by either stimulating ATP-generating and inhibiting ATP-consuming pathways ...
de Almeida Ribeiro E., Pinotsis N., Ghisleni A., Salmazo A., Konarev P. V., Kostan J., Sjöblom B., Schreiner C., Polyansky A., Gkougkoulia E. A., Holt M. R., Aachmann F. L., Žagrović B., Bordignon E., Pirker K. F., Svergun D. I., Gautel M., Djinović-Carugo K. (2014), The structure of human muscle α-actinin: Insight into the intramolecular regulation of ligand binding and Z-disk assembly, Cell 159(6):1447-60. ...
de Almeida Ribeiro E., Pinotsis N., Ghisleni A., Salmazo A., Konarev P. V., Kostan J., Sjöblom B., Schreiner C., Polyansky A., Gkougkoulia E. A., Holt M. R., Aachmann F. L., Žagrović B., Bordignon E., Pirker K. F., Svergun D. I., Gautel M., Djinović-Carugo K. (2014), The structure of human muscle α-actinin: Insight into the intramolecular regulation of ligand binding and Z-disk assembly, Cell 159(6):1447-60. ...
Hee Jeong Son, Yoon Hee Lee, Jeong Hoon Chae, Chang Keun Kim Biol Sport 2015; 32(4):357-361 It is commonly assumed that creatine kinase (CK) activity in plasma is related to a state of an inflammatory response in 24-48 h and ...
article{2910d34e-79d3-4a7a-880b-f6f182695042, abstract = {Objective . Intestinal ischaemia is a life-threatening condition with high mortality, and the lack of accurate and readily available diagnostic methods often results in delay in diagnosis and treatment. The aim of this study was to investigate the accuracy of different plasma biomarkers in diagnosing intestinal ischaemia. Material and methods . Prospective inclusion of patients older than 50 years with acute abdomen admitted to hospital in Karlskrona, Sweden, between 2001 and 2003. Venous blood was sampled prior to any surgery and within 24 h from onset of pain. D-lactate, alpha glutathione S-transferase, intestinal fatty acid binding protein, creatine kinase B, isoenzymes of lactate dehydrogenase (LD) and alkaline liver phosphatase (ALP) were analysed. D-dimer was analysed using four different commercially available test kits. Results . In-hospital mortalities among patients with (n = 10) and without (n = 61) intestinal ischaemia were 40 ...
McQuade and colleagues (1) suggest that achieving predischarge natriuretic peptide (NP) thresholds is associated with reduced heart failure mortality and readmissions. Although this proposed association has biological plausibility, it should be evaluated with caution. One suspects that clinicians would hesitate to discharge patients who seem clinically unstable even if their NP level had decreased. As a result, patients would tend to remain hospitalized longer if their discharge was contingent on achieving both the clinical appearance of stability and a decreasing NP level rather than solely the former. A longer hospital stay in turn would mean that an early relapse would translate into a complicated, prolonged hospitalization rather than a readmission or an early outpatient death. As such, knowing whether the proposed association between a decreasing NP level and better outpatient outcomes persists even after controlling for length of stay would be of interest ...
Hereditary motor and sensory neuropathy associated with agenesis of the corpus callosum (HMSN/ACC; Online Mendelian Inheritance in Man No. 218000) is a severe and progressive autosomal-recessive polyneuropathy associated with hypotonia, mental retardation, dysmorphism, variable degrees of agenesis of the CC, and visual/auditory hallucinations, resulting in death typically during the third decade (Dupré et al., 2003). Our group previously reported that the K+/Cl− cotransporter 3 (KCC3), encoded by the SLC12A6 gene, is disrupted in patients with this disorder (Howard et al., 2002). KCC3 belongs to a family of four K+/Cl− cotransporters that have crucial roles in ion homeostasis, cell volume regulation, and modulation of the cellular responses to GABA during neuronal development (Kahle et al., 2008). Structurally, KCC3 has 12 transmembrane domains and both the N and C termini extend into the cytoplasm (Hiki et al., 1999). The predominant mutation found in HMSN/ACC patients consists of a G ...
INTRODUCTION. Trauma of diverse origins is a common reason for presentation of pets for treatment. It is often difficult clinically to objectively quantify the severity of any trauma suffered by an animal. One approach is to measure changes in the various serum constituents that are known to alter in response to trauma or inflammation. Creatine kinase (CK) is an enzyme found predominantly in skeletal muscle and significantly elevated serum activity is largely associated with muscle damage. It is an extremely sensitive indicator of muscle damage, but is not specific as to cause14,27. Serum elevations in dogs are associated with cell membrane leakage and will therefore be seen in any condition associated with muscular inflammation, necrosis or degeneration. Peak serum activity is expected between 3 and 12 hours after muscular insult and the elevation is roughly proportional to the amount of muscle tissue involved1,5,27.It is also possible to quantify the mass of muscle damage if the changes in CK ...
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Creatine kinase definition, an enzyme that, during muscular activity, catalyzes the transfer of a phosphoryl group from phosphocreatine in muscle to produce ATP. See more.
Signs and symptoms that typically accompany an elevated creatine kinase include cardiac symptoms such as chest pain, breathing trouble, dizziness, excessive sweating and pain that reaches the jaw or...
Expression of CCL16 (CKb12, HCC-4, LCC-1, LEC, LMC, Mtn-1, NCC-4, SCYA16, SCYL4) in seminal vesicle tissue. Antibody staining with in immunohistochemistry.
Creatine+Kinase,+BB+Form at the US National Library of Medicine Medical Subject Headings (MeSH) Human CKB genome location and ... Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein ... Ectopic expression (CKBE) of the B (brain) type of creatine kinase (CK-BB) in red cells and platelets is a rare, benign anomaly ... phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer (CK-BB) in ...
... measures the circulating blood levels of creatine kinase an enzyme found in the heart (CK-MB), brain (CK-BB) and skeletal ... Various forms of family-based treatment have been proven to work in the treatment of adolescent AN including conjoint family ... The lifetime rate of atypical anorexia nervosa, a form of ED-NOS in which the person loses a significant amount of weight and ... Walder A, Baumann P (December 2008). "Increased creatinine kinase and rhabdomyolysis in anorexia nervosa". The International ...
Cytosolic BB-CK, as well as muscle-type MM-CK are both forming banana-shaped symmetric dimers, with one catalytic active site ... Creatine kinase in the blood may be high in health and disease. Exercise increases the outflow of creatine kinase to the blood ... Thus creatine kinase is an important enzyme in such tissues. Clinically, creatine kinase is assayed in blood tests as a marker ... Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phosphocreatine kinase, is an enzyme (EC 2.7.3.2) expressed ...
It has been inferred that levels of CSF neuron-specific enolase, serum NSE, and creatine kinase (type BB) are indicative in the ... In humans there are three subunits of enolase, α, β, and γ, each encoded by a separate gene that can combine to form five ... pyruvate kinase, creatine kinase, and lactate dehydrogenase). The same study showed that the fastest rate of tumor growth ... In human alpha enolase, the two subunits are antiparallel in orientation so that Glu20 of one subunit forms an ionic bond with ...
... itself can be phosphorylated by creatine kinase to form phosphocreatine, which is used as an energy buffer in skeletal ... need phosphocreatine and creatine and indeed kidneys express significant amounts of creatine kinases (BB-CK and u-mtCK ... A cyclic form of creatine, called creatinine, exists in equilibrium with its tautomer and with creatine. Creatine is ... Persky AM, Rawson ES (2007). "Safety of creatine supplementation". Creatine and Creatine Kinase in Health and Disease. ...
Mercer, Donald W (1974). "Separation of tissue and serum creatine kinase isoenzymes by ion-exchange column chromatography". ... This competition led to fast growth of new forms and the fast evolution of IC.[11] A challenge that needs to be overcome in the ... Firstly, CK- MM was eluted, then CK-MB, followed by CK-BB. Therefore, the isoenzymes found in each sample could be used to ... A buffered aqueous solution known as the mobile phase carries the sample from the loop onto a column that contains some form of ...
... creatine kinase MeSH D08.811.913.696.640.150.500 - creatine kinase, bb form MeSH D08.811.913.696.640.150.625 - creatine kinase ... creatine kinase, mitochondrial form MeSH D08.811.913.696.640.150.875 - creatine kinase, mm form MeSH D08.811.913.696.645 - ... map kinase kinase kinase 1 MeSH D08.811.913.696.620.682.700.559.200 - map kinase kinase kinase 2 MeSH D08.811.913.696.620.682. ... map kinase kinase kinase 3 MeSH D08.811.913.696.620.682.700.559.400 - map kinase kinase kinase 4 MeSH D08.811.913.696.620.682. ...
... protein kinase A). AMPK is a heterotrimeric protein complex that is formed by α, β, and γ subunits. Each of these three ... Thomson DM, Porter BB, Tall JH, Kim HJ, Barrow JR, Winder WW (January 2007). "Skeletal muscle and heart LKB1 deficiency causes ... expression in skeletal muscle in response to creatine depletion. PGC-1α is a transcriptional regulator for genes involved in ... liver kinase B1 (LKB1), which works in a complex with STRAD and MO25, Calcium/calmodulin-dependent protein kinase kinase II-( ...
Protein kinase B / Akt is thought to downregulate PGC-1α, but upregulate its downstream effectors, NRF1 and NRF2. Akt itself is ... Lin J, Wu H, Tarr PT, Zhang CY, Wu Z, Boss O, Michael LF, Puigserver P, Isotani E, Olson EN, Lowell BB, Bassel-Duby R, ... Among them creatine, Coenzyme Q10 and mitochondrial targeted antioxidants/peptides are reported to have the most remarkable ... both formed endogenously in the cell as by-products of metabolism but upregulated during times of cellular stress. It is ...
Salvadori A, Fanari P, Ruga S, Brunani A, Longhini E (1992). "Creatine kinase and creatine kinase-MB isoenzyme during and after ... क्रियेटीन काइनेज़ टेस्ट (CK-टेस्ट): इसमें हृदय (CK-MB), मस्तिष्क (CK-BB) और मांसपेशियों (CK-MM) में पाए जाने वाले क्रियेटीन ... Lucka I, Cebella A (2004). "[Characteristics of the forming personality in children suffering from anorexia nervosa]". ... Walder A, Baumann P (2008). "Increased creatinine kinase and rhabdomyolysis in anorexia nervosa". The International Journal of ...
Salvadori A, Fanari P, Ruga S, Brunani A, Longhini E (1992). «Creatine kinase and creatine kinase-MB isoenzyme during and after ... Kreatinkinase er et enzym som finnes i hjertet (CK-MB), i hjernen (CK-BB) og i skjelettmuskulaturen (CK-MM).[105][106] ... Lucka I, Cebella A (2004). «[Characteristics of the forming personality in children suffering from anorexia nervosa]». ... Walder A, Baumann P (2008). «Increased creatinine kinase and rhabdomyolysis in anorexia nervosa». The International Journal of ...
An autosomal dominant form has been associated with mutations in the LRP10 gene. About 5% of people with PD have mutations in ... Tremlett H, Bauer KC, Appel-Cresswell S, Finlay BB, Waubant E (March 2017). "The gut microbiome in human neurological disease: ... Alpha-synuclein activates ATM (ataxia telangiectasia mutated), a major DNA damage repair signaling kinase. In addition, alpha- ... creatine), calcium channel blockers (isradipine) and growth factors (GDNF). Reducing alpha-synuclein pathology is a major focus ...
Fredholm BB, Bättig K, Holmén J, Nehlig A, Zvartau EE (March 1999). "Actions of caffeine in the brain with special reference to ... Caffeine can produce a mild form of drug dependence - associated with withdrawal symptoms such as sleepiness, headache, and ... S2CID 378245.CS1 maint: uses authors parameter (link) Tarnopolsky MA (2010). "Caffeine and creatine use in sport". Annals of ... As a competitive nonselective phosphodiesterase inhibitor, caffeine raises intracellular cAMP, activates protein kinase A, ...
The GTP that is formed by GDP-forming succinyl-CoA synthetase may be utilized by nucleoside-diphosphate kinase to form ATP (the ... Jones RC, Buchanan BB, Gruissem W (2000). Biochemistry & molecular biology of plants (1st ed.). Rockville, Md: American Society ... or used to form ketone bodies, which too can only be burned in tissues other than the liver where they are formed, or excreted ... In mammals a GTP-forming enzyme, succinate-CoA ligase (GDP-forming) (EC 6.2.1.4) also operates. The level of utilization of ...
doi:10.1146/annurev.bb.15.060186.000525. PMID 3013232.. *^ a b Gillen JB, Martin BJ, MacInnis MJ, Skelly LE, Tarnopolsky MA, ... This forms a tetrahedral intermediate and results in the ejection of −SCoA as the carbonyl reforms. The −SCoA is protonated to ... Adenylate kinase. *Creatine kinase. Inner membrane. oxidative phosphorylation. *Coenzyme Q - cytochrome c reductase ... Only when this citroyl-CoA has formed will another conformational change cause thioester hydrolysis and release coenzyme A. ...
Testing for the MB form of creatine kinase provides information about the heart's blood supply, but is used less frequently ... e f g h i j k l m n o p q r s t u v w x y z aa ab ac ad ae af ag ah ai aj ak al am an ao ap aq ar as at au av aw ax ay az ba bb ... Two endocardial tubes form here that fuse to form a primitive heart tube known as the tubular heart.[35] Between the third and ... In tetrapods, the ventral aorta has divided in two; one half forms the ascending aorta, while the other forms the pulmonary ...
Reduction of coenzyme Q from its ubiquinone form (Q) to the reduced ubiquinol form (QH2). ... regulated by Pyruvate dehydrogenase kinase and Pyruvate dehydrogenase phosphatase). to α-ketoglutaric acid. *Glutamate ... Creatine. & Polyamines Ketogenic &. Glucogenic. Amino Acids Amino Acids Shikimate Aromatic Amino. Acids & Histidine ...
Salvadori A, Fanari P, Ruga S, Brunani A, Longhini E (1992). "Creatine kinase and creatine kinase-MB isoenzyme during and after ... ಕ್ರಿಯಟಿನ್ ಕಿನೇಸ್ ಪರೀಕ್ಷೆ(CK-Test): ಕ್ರಿಯಟಿನ ಕಿನೇಸ್ ಎಂಬ ಒಂದು ಕಿಣ್ವವು ಹೃದಯದಲ್ಲಿ (CK-MB), ಮೆದುಳಿ (CK-BB) ನಲ್ಲಿ ಮತ್ತು ಅಸ್ಥಿಪಂಜರ ... Lucka I, Cebella A (2004). "[Characteristics of the forming personality in children suffering from anorexia nervosa]". ... Walder A, Baumann P (2008). "Increased creatinine kinase and rhabdomyolysis in anorexia nervosa". The International Journal of ...
Creatine Kinase, BB Form / metabolism * Energy Metabolism * Extracellular Matrix * Gene Expression Regulation, Neoplastic ... These miRNAs convergently target creatine kinase, brain-type (CKB), which phosphorylates the metabolite creatine, to generate ...
1999 [5]). Cytosolic BB-CK, as well as muscle-type MM-CK are both forming banana-shaped symmetric dimers, with one active site ... Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phosphocreatine kinase, is an enzyme (EC 2.7.3.2) expressed ... Creatine kinase in the blood may be high in health and disease. Exercise increases the outflow of creatine kinase to the blood ... Thus creatine kinase is an important enzyme in such tissues.[3] Clinically, creatine kinase is assayed in blood tests as a ...
Shop a large selection of All Primary Antibodies products and learn more about Creatine Kinase BB, Mouse anti-Human, DyLight ... Please use the form below to provide feedback related to the content on this product. ... Creatine Kinase BB Monoclonal antibody specifically detects Creatine Kinase BB in Human samples. It is validated for Flow ... Creatine Kinase BB, Mouse anti-Human, DyLight 350, Clone: CKB/1268, Novus Biologicals™ ...
Signs and symptoms that typically accompany an elevated creatine kinase include cardiac symptoms such as chest pain, breathing ... CK-MM is a form of creatine kinase found in the heart and skeletal muscles, whereas CK-MB is present in the heart. The type of ... creatine kinase located in the brain is called CK-BB.. A higher creatine kinase level usually manifests a few hours after a ... The heart, brain and skeletal muscles are the parts of the body that primarily contain the enzyme creatine kinase, states ...
In addition to those, there are two mitochondrial creatine kinases, the ubiquitous and sarcomeric form. ... CK-BB occurs mainly in tissues, and its levels do rarely have any significance in bloodstream. Skeletal muscle expresses CK-MM ... Creatine kinase (CK), also known as phosphocreatine kinase or creatine phosphokinase (CPK) is an enzyme (EC 2.7.3.2) expressed ... Thus Creatine Kinase is an important enzyme in such tissues. Clinically, creatine kinase is assayed in blood tests as a marker ...
... are based mostly on the functions of the enzyme creatine kinase (CK) and its high-energy product phosphocreatine (PCr). ... form homo-dimers or hetero-dimers, e.g. MM-CK in skeletal muscle, MM-, MB- and BB-CK in heart, or BB-CK in brain, kidney, ... Molecular structure and function of mitochondrial creatine kinases. In: Vial C (ed), Uversky VN (series ed) Creatine kinase- ... High content of creatine kinase in chicken retina: compartmentalized localization of creatine kinase isoenzymes in ...
... creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating ... With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, ... PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. ... PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. ...
... creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating ... Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form ... in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.By similarity. ... PS00112. PHOSPHAGEN_KINASE. 1 hit. PS51510. PHOSPHAGEN_KINASE_C. 1 hit. PS51509. PHOSPHAGEN_KINASE_N. 1 hit. ...
... measures the circulating blood levels of creatine kinase an enzyme found in the heart (CK-MB), brain (CK-BB) and skeletal ... The lifetime rate of atypical anorexia nervosa, a form of ED-NOS in which not all of the diagnostic criteria for AN are met, is ... Walder A, Baumann P (2008). "Increased creatinine kinase and rhabdomyolysis in anorexia nervosa". The International Journal of ... Blood urea nitrogen (BUN) test: urea nitrogen is the byproduct of protein metabolism first formed in the liver then removed ...
CKBB creatine kinase B-type; creatine kinase, brain; CKBB; Brain creatine kinase; Creatine kinase B-type; Creatine Kinase BB ... Ester dyes are stable in solid form as long as they are protected from light and moisture. Esters are not stable in aqueous ... B-CK; BB-CK; creatine kinase-B; Creatine kinase B chain; ... Anti-Creatine Kinase-BB (CKBB/1268) Antibody # prefix. ... Creatine Kinase B Monoclonal Mouse Antibody (CKBB/1268) Creatine kinases (CK) are a large family of isoenzymes that regulate ...
Rabbit polyclonal Creatine kinase B type antibody validated for WB, ELISA and tested in Human and Mouse. Referenced in 5 ... looking for Ab specific to Creatine Kinase BB, MM and MB that does not crossreact with the other forms check homology human and ... Anti-Creatine kinase B type antibody. See all Creatine kinase B type primary antibodies. ... A KLH conjugated synthetic peptide selected within aa 200~300 of human Creatine Kinase BB. ...
... that binds human creatine Kinase MB (also known as CKMB). ... Creatine Kinase MB antibody LS-C193283 is a protein G-purified ... there are two mitochondrial creatine kinase isoenzymes, the ubiquitous and sarcomeric form. (More About CKMB / Creatine Kinase ... CK-BB and CK-MB. The genes for these subunits are located on different chromosomes: B on 14q32 and M on 19q13. In addition to ... Most Popular CKMB / Creatine Kinase MB Antibodies. Anti-CKMB / Creatine Kinase MB Antibody LS-C14666 ...
Creatine kinase is a dimer occurring in various in three isoenzymic forms, depending on the particular combination of its non- ... identical subunits: BB (brain type); MM (skeletal type); and MB(hybrid type). Creatine kinase-MB isoenzyme is released into ... The sample continues to move to the control area and forms a pink to purple color, indicating the test is working and the ...
... creatine kinase (CK: three isozymes of CK-MM derived from skeletal muscle, CK-BB derived from smooth muscle, and CK-MB derived ... open ring-like configuration (C letter form).. FIG. 2. is a schematic diagram showing a form of the measuring probe in Example. ... The configuration may be a non-linear form or an open ring-form (C letter configuration) as shown in FIGS. 1D and 1E. . ... Binder resin containing polyhydroxyalkanoate, toner containing the binder resin, and image-forming method and image-forming ...
... evidenced by the leakage of creatine kinase-BB, was not seen, the jugular bulb venous hemoglobin saturation levels (,50% in 27 ... evidenced by the leakage of creatine kinase-BB, was not seen, the jugular bulb venous hemoglobin saturation levels (,50% in 27 ... evidenced by the leakage of creatine kinase-BB, was not seen, the jugular bulb venous hemoglobin saturation levels (,50% in 27 ... evidenced by the leakage of creatine kinase-BB, was not seen, the jugular bulb venous hemoglobin saturation levels (,50% in 27 ...
The recombinant BB-CK protein was purified to over 99% homogeneity from the total protein extract of AcDZ1-CKB infected cells ... formed up to 30% of the total soluble cellular protein. Upon analysis by PAGE, zymogram assay and gel filtration chromatography ... A full-length human creatine kinase B (B-CK) cDNA was used to produce a recombinant baculovirus (AcDZ1-BCK). Sf9 cells infected ... the recombinant protein behaved like authentic dimeric human BB-CK protein. Studies with a newly produced monoclonal antibody ( ...
... topographical interaction with the Na+-K+ ATPase and its modulators such as creatine kinase should modulate functions of Cl- ... In addition, activation of these kinases is modulated by humoral factors such as estrogen and taurine. Because these ... KCC2 and NKCC1 functions are also regulated by phosphorylation by enzymes such as PKC, Src-family tyrosine kinases, and WNK1-4 ... BB (the predominant form in brain), MM (in skeletal muscle) and MB (in cardiac muscle). Of these, only CKB interacts with KCC2 ...
Rabbit recombinant monoclonal Creatine kinase B type antibody [EPR3926] validated for WB, IP, IHC, ICC, Flow Cyt and tested in ... ab108388, at 1/100 dilution, staining Creatine Kinase BB in Human heart by Immunohistochemistry, Paraffin-embedded tissue. ... Anti-Creatine kinase B type antibody [EPR3926] (HRP) (ab197066) *Anti-Creatine kinase B type antibody [EPR3926] (Biotin) ( ... Anti-Creatine kinase B type antibody [EPR3926]. See all Creatine kinase B type primary antibodies. ...
Creatine kinase Mb (CKMB) Clinical Use:. Background:. CK-MB is a dimeric enzyme. Three different iso-forms occur in normal ... circulation: CK-MM (muscle type), CK-BB (brain type) and CK-MB that is primarily associated with the myocardium. ... should match on the specimen and request form. This check may be performed prior to centrifugation by the Central Sort ...
CK-BB) Isoenzyme Recombinant. Full length Creatine Kinase BBisoenzyme with amino acid sequence identical to the native enzyme. ... Purified in theenzymatically active form ... creatine kinase B-type; creatine kinase-B; creatine kinase B ... Human Creatine Kinase BB Isoenzyme (DAG279) Human Creatine Kinase BB Isoenzyme, recombinant protein from P. pastoris Datasheet ... CreatineKinase BB (CK-BB) Isoenzyme Recombinant. Full length Creatine Kinase BBisoenzyme with amino acid sequence identical to ...
Unmasking artifactual increases in creatine kinase isoenzymes in patients with renal failure. Together they form a unique ... Unmasking artifactual increases in creatine kinase isoenzymes in patients with renal failure. ...
BB Form Creatine Kinase * Chaperonins * Protein Disulfide-Isomerases * Electrophoresis * Mass spectrometry * Glial Fibrillary ... Together they form a unique fingerprint. * Sort by ...
Chicken heart muscle contains almost exclusively the BB isoenzyme of creatine kinase (CK), its myofibrils, moreover, lack an M- ... Chicken heart myofibrils may thus possess binding sites for both M and B forms of CK. ... Localization of creatine kinase isoenzymes in myofibrils. II. Chicken heart muscle. T Wallimann T Wallimann ... Localization of creatine kinase isoenzymes in myofibrils. I. Chicken skeletal muscle. Isoenzyme-Specific Interaction of Muscle- ...
Creatine Kinase, BB Form - metabolism , Creatine Kinase, BB Form - genetics , Creatine Kinase, BB Form - chemistry , Proteomics ... Creatine Kinase, MM Form - chemistry , Creatine Kinase, BB Form - genetics , Creatine Kinase, BB Form - chemistry , Protein ... Creatine Kinase, BB Form - metabolism , Mice, Knockout , Bone Resorption - enzymology , Creatine Kinase, BB Form - genetics , ... Creatine Kinase, BB Form - biosynthesis , Creatine Kinase, BB Form - genetics , Neoplasm Metastasis - genetics , Animals , Mice ...
MM creatine kinase. Using a polyclonal antisera, we have localized BB creatine kinase to the cuboidal epithelial cells of the ... In human muscle creatine kinase (HMCK) this residue, Cys283, forms part of a conserved cysteine-proline-serine (CPS) motif and ... Several physical properties of creatine kinase (EC 2.7.3.2) isozymes MM (CK-MM, muscle-type) and BB (CK-BB, brain-type), both ... Brain-type creatine kinase BB-CK interacts with the Golgi Matrix Protein GM130 in early prophase.. Bürklen TS, Hirschy A and ...
Creatine Kinase, BB Form/blood , Creatine Kinase, BB Form/drug effects , Gene Expression , Heart Ventricles/drug effects , ... creatine kinase muscle-brain fraction (CK-MB) and gene expressions of SERCA2a, phospholamban (PLB) and ryanodine (RyR). Results ...
BB Form Creatine Kinase Medicine & Life Sciences * Brain Medicine & Life Sciences * Mitochondrial Form Creatine Kinase Medicine ... Creatine kinase-catalyzed reaction rate in the cyanide-poisoned mouse brain. Together they form a unique fingerprint. * ... Creatine kinase-catalyzed reaction rate in the cyanide-poisoned mouse brain. In: Journal of Cerebral Blood Flow and Metabolism ... Creatine kinase-catalyzed reaction rate in the cyanide-poisoned mouse brain. Journal of Cerebral Blood Flow and Metabolism. ...
BB Form Creatine Kinase Medicine & Life Sciences * Myosin-Light-Chain Kinase Medicine & Life Sciences ... Decreased Creatine Kinase Activity in Cultured Duchenne Dystrophic Muscle Cells. Together they form a unique fingerprint. * ... Decreased Creatine Kinase Activity in Cultured Duchenne Dystrophic Muscle Cells. / Iannaccone, S. T.; Nagy, B.; Samaha, F. J. ... Decreased Creatine Kinase Activity in Cultured Duchenne Dystrophic Muscle Cells. Journal of child neurology. 1987 Jan;2(1):17- ...
Creatine Kinase consists of the M and B subunits and is found in the MM, MB and BB isomorphs. The CK-MB isomorph is found ... Form: Freeze-dried. Shelf life: 24 months @ 2-8 C. On-Board Stability: 14 days Sample: Non lipemic, non hemolyzed human serum. ...
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  • Creatine kinase ( CK ), also known as creatine phosphokinase ( CPK ) or phosphocreatine kinase , is an enzyme ( EC 2.7.3.2 ) expressed by various tissues and cell types. (wikipedia.org)
  • Creatine kinase is also sometimes referred to as creatine phosphokinase, creatine phosphotransferase, CPK, or just CK. (aaltoscientific.com)
  • 1. Narayanaswami, A. Creatine Phosphokinase in Mammalian Brain. (aaltoscientific.com)
  • Regulation of creatine phosphokinase B activity by protein kinase C. (semanticscholar.org)
  • It is also known as CPK (Creatine phosphokinase) MB test. (1mg.com)
  • Creatine phosphokinase (CPK) is widely distributed and is primarily involved with ATP regeneration in conjunction with the contractile and transport systems. (creative-enzymes.com)
  • All three of these isoenzymes make up the main enzyme CPK (creatine phosphokinase) (also called CK--creatine kinase). (nurseslearning.com)
  • Most often there is a dramatic increase in a skeletal muscle enzyme known as creatine kinase or creatine phosphokinase (CPK-MM). Smaller amounts of this enzyme are also located in the brain (CPK-BB) and in the heart muscle (CPK-MB). (drjwv.com)
  • The creatine phosphokinase (CPK) isoenzymes test measures the different forms of CPK in the blood. (medlineplus.gov)
  • Creatine phosphokinase (CPK) is an enzyme in the body. (adam.com)
  • Whereas MM-CK is expressed in sarcomeric muscle, that is, skeletal and cardiac muscle, MB-CK is expressed in cardiac muscle, and BB-CK is expressed in smooth muscle and in most non-muscle tissues. (wikipedia.org)
  • The heart, brain and skeletal muscles are the parts of the body that primarily contain the enzyme creatine kinase, states Healthgrades. (reference.com)
  • Increased creatine levels often accompany heart attacks, trauma, stroke, brain injury and conditions that impair the brain or skeletal muscles. (reference.com)
  • CK-MM is a form of creatine kinase found in the heart and skeletal muscles, whereas CK-MB is present in the heart. (reference.com)
  • Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. (uniprot.org)
  • Very high levels of CK are found in skeletal muscle, primarily the MM form. (aaltoscientific.com)
  • While CK MM is found in skeletal muscles and heart, CK BB is found in the brain and smooth muscles. (1mg.com)
  • The production of creatine occurs primarily in the kidneys and the liver, but 95% of it is found in the skeletal muscles. (kidneypedia.net)
  • CK-MM is the predominant form of CK in skeletal muscle. (vitroscient.com)
  • With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. (abcam.cn)
  • Dual regulation of the AMP-activated protein kinase provides a novel mechanism for the control of creatine kinase in skeletal muscle. (semanticscholar.org)
  • Creatine kinase isoenzyme activities in extracts of plasma, skeletal muscle, heart and brain tissue of domestic fowls were separated by anion exchange chromatography and tissue specific distributions of the isoenzyme designated MM-CK, BB-CK1 and BB-CK2 were demonstrated. (sruc.ac.uk)
  • Depending on the tissue, different forms of CK are present: CK-MM is primarily present in skeletal muscles, CK-MB represents 30% of CK from the heart, while CK-BB comes from the brain and smooth muscle, such as the intestinal walls. (biron.com)
  • Creatine kinase isoenzymes from Torpedo californica electric organ, skeletal muscle, and brain were purified and characterized. (mysciencework.com)
  • Torpedo electric organ and skeletal muscle creatine kinase have identical apparent Mr, electrophoretic mobility, and cyanogen bromide fragments. (mysciencework.com)
  • Western blot analysis showed Torpedo electric organ skeletal muscle creatine kinase and acetylcholine receptor-enriched membrane creatine kinase reacted with antiserum specific for canine MM creatine kinase. (mysciencework.com)
  • Creatine kinase (CK) is an enzyme present in cardiac and skeletal muscle that is released into the blood when cells are injured. (pathologystudent.com)
  • CK-BB predominates in brain, CK-MB in cardiac muscle, while CK-MM in skeletal muscle and heart. (com.pk)
  • Chicken heart muscle contains almost exclusively the BB isoenzyme of creatine kinase (CK), its myofibrils, moreover, lack an M-line. (rupress.org)
  • CK MB is a form of the enzyme which means it is an isoenzyme of Creatine kinase (CK). (1mg.com)
  • The MB Isoenzyme of Creatine Kinase as an Indicator of Severity of Myocardial Ischaemia. (aaltoscientific.com)
  • CK-MB is the isoenzyme of Creatine Kinase most involved in the metabolism of cardiac muscle tissue. (custom-monoclonalantibody.com)
  • The protein encoded by this gene, CK-BB, consists of a homodimer of two identical brain-type CK-B subunits. (wikipedia.org)
  • The encoded protein reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. (wikipedia.org)
  • The encoded CK-BB and CK-MB proteins are members of the ATP:guanido phosphotransferase protein family. (wikipedia.org)
  • Sf9 cells infected with this recombinant expressed a homodimeric protein composed of 43 kDa subunits which, under optimal conditions, formed up to 30% of the total soluble cellular protein. (semanticscholar.org)
  • Upon analysis by PAGE, zymogram assay and gel filtration chromatography the recombinant protein behaved like authentic dimeric human BB-CK protein. (semanticscholar.org)
  • Expression of active secreted forms of human amyloid beta-protein precursor by recombinant baculovirus-infected insect cells. (semanticscholar.org)
  • 2. The measuring probe according to claim 1 , wherein the protein layer is formed to have a portion fully encircling an axis of elongation of the fiber-shaped portion. (google.com.au)
  • Whereas many of the identified proteins have been previously implicated in schizophrenia, such as fructose-bisphosphate aldolase C, creatine kinase and neuron-specific enolase, new putative disease markers were also identified such as dihydrolipoyl dehydrogenase, tropomyosin 3, breast cancer metastasis-suppressor 1, heterogeneous nuclear ribonucleoproteins C1/C2 and phosphate carrier protein, mitochondrial precursor. (genes2cognition.org)
  • Protein and myosin content and pyruvate kinase (PK) activity were similar in normal and Duchenne muscular dystrophy cultures. (elsevier.com)
  • Includes C-Reactive Protein (CRP), Rheumatoid Arthritis (RA) Factor, Antinuclear Antibody(ANA), Sedimentation Rate, Uric Acid plus Wellness #2 Essential Panel that includes Complete Blood Count (CBC), Comprehensive Metabolic Panel (CMP-14), Lipid Panel with Total Cholesterol: HDL Ratio, Thyroid Panel plus TSH plus Cyclic Citrullinated Peptide (CCP) Antibodies, Creatine Kinase (CPK), Aldolase and Prostate-specific Antigen. (walkinlab.com)
  • In AD pathology, the tau protein undergoes intense hyperphosphorylation, leading to the clumping of tau protein, which forms intracellular neurofibrillary tangles (NFT). (hindawi.com)
  • CKM (Creatine Kinase, M-Type) is a Protein Coding gene. (genecards.org)
  • CK (creatine kinase) is an enzyme (protein) found in several tissues, including muscles and the heart. (biron.com)
  • Amyloid beta-peptide (1-40)-mediated oxidative stress in cultured hippocampal neurons: protein carbonyl formation, CK BB expression, and the level of Cu, Zn, and Mn SOD mRNA. (springer.com)
  • platelet derived growth factor receptor protein which preferentially binds to the AA homodimer and AB heterodimer forms of platelet derived growth factor and also binds the BB homodimer at high affinity, is described. (thefreedictionary.com)
  • Expression of creatine kinase protein and mRNA was lower in MCT rats compared to CON, with a trend for reversion by BB treatment. (bris.ac.uk)
  • Creatine Kinase consists of the M and B subunits and is found in the MM, MB and BB isomorphs. (mediconsa.com)
  • Creatine kinase is composed of M and B subunits and may be traced in the brain, muscle, and heart. (docksci.com)
  • Creatine kinase (ATP: Creatine N-phosphotransferase, EC2.7.3.2) is a dimeric enzymecomposed of two types of monomer subunits, M (Muscular) and B (Brain). (vitroscient.com)
  • The subunits combine to form three distinct CK. (vitroscient.com)
  • The subunits combine to form three distinct CK isoenzymes, CK-BB (CK-1), CK-MB (CK-2) and CK-MM (CK-3). (vitroscient.com)
  • Creatine Kinase exists as dimeric molecules composed of M and B subunits that form the isoenzymes MM, MB, and BB. (pointescientific.com)
  • These subunits associate to form three isoenzymic forms: CK-BB, CK-MM and CK-MB. (avant-medical.com)
  • A traditional immunoassay that could distinguish CKMB from CKBB and CKMM was a little longer in coming due to the fact that CKMB has no unique subunits compared to these other forms. (aaltoscientific.com)
  • Creatine Kinase MB (CK-MB) is an enzyme present in the cardiac muscle with a molecular weight of 87.0 kDa.1Creatine Kinase is a dimeric molecule formed from two subunits designated as "M" and "B" which combine to form three different isoenzymes, CK-MM, CK-BB, and CK-MB. (custom-monoclonalantibody.com)
  • Hybridization studies demonstrate the Torpedo electric organ creatine kinase to be composed of identical subunits and capable of producing an enzymatically active heterodimer when combined with canine BB creatine kinase. (mysciencework.com)
  • The acetylcholine receptor-associated creatine kinase is, therefore, identical with the cytoplasmic form from the electric organ and is composed of M-subunits. (mysciencework.com)
  • The exercise protocol promoted slight muscle damage, reflected in significant increases in total creatine kinase in all subjects after exercise. (biomedcentral.com)
  • Previously, total creatine kinase, LD, and AST were used for the diagnosis of MI. (com.pk)
  • Elevated CKMB with normal total creatine kinase in suspected myocardial infarction: Associated clinical findings and early prognosis. (openwetware.org)
  • This MAb recognizes the CKBB (Creatine Kinase B) isoenzyme and does not react with the B subunit in CKMB. (fishersci.com)
  • The key cardiac enzymes are, creatine kinase muscle/brain (CKmb/CPKmb), troponin I (TnI) and troponin T (TnT). (dynamicdeism.org)
  • In addition to those three cytosolic CK isoforms, there are two mitochondrial creatine kinase isoenzymes, the ubiquitous and sarcomeric form. (wikipedia.org)
  • While mitochondrial creatine kinase is directly involved in formation of phospho-creatine from mitochondrial ATP, cytosolic CK regenerates ATP from ADP, using PCr. (wikipedia.org)
  • The first X-ray structure of a creatine kinase (CK) family member solved was that of sarcomeric muscle-type mitochondrial CK (s-mtCK (in 1996) (Fritz-Wolf et al. (wikipedia.org)
  • The mitochondrial creatine kinase (CK m ) is present in the mitochondrial intermembrane space, where it regenerates phosphocreatine (PCr) from mitochondrially generated ATP and creatine (Cr) imported from the cytosol . (wikipedia.org)
  • Apart from the two mitochondrial CK isoenzyme forms, that is, ubiquitous mtCK (present in non-muscle tissues) and sarcomeric mtCK (present in sarcomeric muscle), there are three cytosolic CK isoforms present in the cytosol, depending on the tissue. (wikipedia.org)
  • The mitochondrial creatine kinase (CK m ), which produces ATP from ADP by converting creatine phosphate to creatine, is present between the two membranes of the mitochondrion. (wikidoc.org)
  • There is a third gene product which results in the mitochondrial form of CK. (aaltoscientific.com)
  • Mitochondrial creatine kinase: a key enzyme of aerobic energy metabolism. (semanticscholar.org)
  • Mitochondrial creatine kinase in human health and disease. (semanticscholar.org)
  • The biological role of the brain specific creatine kinase energy system in mice. (semanticscholar.org)
  • 1993) CSF and serum brain-specific creatine kinase isoenzyme (CK-BB), neuron-specifi cenolase (NSE), and neural cell adhesion molecule (NCAM) asprognostic markers for hypoxic brain injury after cardiac arrestin man. (qrudestudio.com)
  • Interestingly, other forms of cancer have also resulted in clinically significant levels of elevated CKBB in serum. (aaltoscientific.com)
  • Clinically, creatine kinase is assayed in blood tests as a marker of damage of CK-rich tissue such as in myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy , autoimmune myositides , and acute kidney injury . (wikipedia.org)
  • CK-BB is predominantly expressed in brain and smooth muscle, including vascular and uterine tissue. (wikipedia.org)
  • Cytosolic BB-CK, as well as muscle-type MM-CK are both forming banana-shaped symmetric dimers, with one active site in each subunit. (wikipedia.org)
  • A higher creatine kinase level usually manifests a few hours after a heart attack when the heart muscle cells start to die, says Healthgrades. (reference.com)
  • Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy and in acute renal failure . (wikidoc.org)
  • Creatine (Cr) has emerged as a safe nutritional supplement not only to increase muscle mass and performance, prevent disease-induced muscle atrophy and improve rehabilitation, but also to strengthen cellular energetics in general (see Salomons and Wyss 2007). (springer.com)
  • Its functional entity is a homodimer (CK-BB) in brain and smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone, etc. (wikipedia.org)
  • Three different iso-forms occur in normal circulation: CK-MM (muscle type), CK-BB (brain type) and CK-MB that is primarily associated with the myocardium. (southtees.nhs.uk)
  • After the experimental period, the following variables were analyzed: final body weight (FBW), left ventricular weight (LVW), LVW/FBW ratio, cardiomyocyte cross-sectional area (CSA), collagen fraction, creatine kinase muscle-brain fraction (CK-MB) and gene expressions of SERCA2a, phospholamban (PLB) and ryanodine (RyR). (bvsalud.org)
  • Creatine kinase is an enzyme produced by various tissues that catalyzes the conversion of creatine in muscle activity. (kidneypedia.net)
  • There are two main recognized types of creatine kinase, type B and type M. The B and M stand respectively for "brain" and "muscle. (kidneypedia.net)
  • Normal creatine kinase levels vary by age, gender, muscle mass, and level of activity. (kidneypedia.net)
  • Other possible causes of high serum creatine kinase include muscular dystrophy, severe muscle breakdown, and several other rarer causes. (kidneypedia.net)
  • Lee Biosolutions is the leading manufacturer of human muscle Creatine Kinase MM (CKMM,CK-MM) for medical research and diagnostic manufacturing. (leebio.com)
  • Creatine Kinase (CK) is released from the muscles whenever there is muscle damage. (esamievalori.com)
  • CK-BB mainly located in the brain and smooth muscle: intestine, uterus and prostate. (esamievalori.com)
  • CK-BB is found in brainand smooth muscle. (vitroscient.com)
  • The enzyme is dimeric and exists in three isoenzyme forms: MM (muscle), MB (heart) and BB (brain). (creative-enzymes.com)
  • CK-MM and CK-MB are distributed primarily in the skeletel muscle and heart muscle, respectively, while CK-BB is present mainly in the brain and in tissues composed of smooth muscle. (pointescientific.com)
  • Creatine Kinase (CK) is a dimeric enzyme that catalyzes the conversion of creatine to creatine phosphate, it is composed of two sub-units Muscle (M) and Brain (B) which associate to form three isoenzymes CK-MM, CK-MB and CK-BB. (randoxonlinestore.com)
  • The muscle isoenzyme (MM-CK) was the predominant form in plasma (99 percent) and its activity increased in response to an episode of acute heat stress. (sruc.ac.uk)
  • Muscle-type MM creatine kinase is specifically boundto sarcoplasmic reticulum and can support Ca2+ uptake and regulate local ATP/ADP ratios. (thefreedictionary.com)
  • This enzyme has a short life span in the serum and is most often elevated in the acute form of the disease due to the magnitude of muscle damage and because the owner usually presents the dog while the disease is present. (drjwv.com)
  • Athletes and others who engage in strenuous physical activity have higher creatine kinase without it being a sign of anything necessarily wrong with them. (kidneypedia.net)
  • Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. (wikipedia.org)
  • Creatine+Kinase,+BB+Form at the US National Library of Medicine Medical Subject Headings (MeSH) Human CKB genome location and CKB gene details page in the UCSC Genome Browser. (wikipedia.org)
  • Clinical Applications of Microarray Technology: Creatine Kinase B is an Up-regulated Gene in Epithelial Ovarian Cancer and Shows Promise as a Serum Marker. (aaltoscientific.com)
  • GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and creatine kinase activity . (genecards.org)
  • Reporter-plasmids are transfected into thymocytes in fetal thymic organ culture by accelerated DNA/particle bombardment (gene gun), and the activation of a signaling pathway is determined in the form of a standard luciferase assay. (storysteel.cf)
  • Alleles are forms of the same gene with small differences in their sequence of DNA bases. (weebly.com)
  • Creatine Kinase BB Monoclonal antibody specifically detects Creatine Kinase BB in Human samples. (fishersci.com)
  • This antibody is specific for Creatine kinase BB. (abcam.com)
  • Almost all of the bound enzyme is located within the Z-line region of each sarcomere, as revealed by indirect fluorescent-antibody staining with antiserum against purified chicken BB-CK. (rupress.org)
  • This antibody completely inhibits the activity of CK-MM and half of the activity of CK-MB, while not affecting the B subunit activity of CK-MB and CK-BB. (pointescientific.com)
  • The ELISA analytical biochemical technique of the MBS706326 kit is based on CK-BB antibody-CK-BB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CK-BB antigen targets in samples. (mybiosource.com)
  • Antibody specific for CK-BB has been pre-coated onto a microplate. (mybiosource.com)
  • Standards and samples are pipetted into the wells and any CK-BB present is bound by the immobilized antibody. (mybiosource.com)
  • After removing any unbound substances, a biotin-conjugated antibody specific for CK-BB is added to the wells. (mybiosource.com)
  • CK catalyses the conversion of creatine and utilizes adenosine triphosphate (ATP) to create phosphocreatine (PCr) and adenosine diphosphate (ADP). (wikipedia.org)
  • [2] Thus creatine kinase is an important enzyme in such tissues. (wikipedia.org)
  • CK-BB occurs mainly in tissues, and its levels do rarely have any significance in bloodstream. (wikidoc.org)
  • CK-BB is expressed in all tissues at low levels and has little clinical relevance. (wikidoc.org)
  • Intracellular compartmentation, structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands: the 'phosphocreatine circuit' for cellular energy homeostasis. (semanticscholar.org)
  • No creatine kinase isoenzyme with an electrophoretic mobility similar to mammalian BB creatine kinase was seen in any of the Torpedo tissues examined. (mysciencework.com)
  • Recombinant creatine kinase proteins and proposed standards for creatine kinase isoenzyme and subform assays. (semanticscholar.org)
  • Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). (uniprot.org)
  • One unit will transfer one micromoleof phosphate from creatine phosphate to ADP per minute at 37 o C. Measured at340nm as one equimolar amount of NADH produced by coupled reaction. (creative-diagnostics.com)
  • Creatine Kinase MB reversiblycatalyses the transfer of phosphate between ATP and various phosphogens. (creative-diagnostics.com)
  • One unit will catalyze the transphosphorylation of one micromole of phosphate from creatine phosphate to ADP per minute at 37°C. (leebio.com)
  • The determination of CK using creatine phosphate and adenosine-5'-diphosphate (ADP) as substrates rather than creatine and adenosine-5'-triphosphate (ATP) has several advantages in test performance as it allows for a faster reaction rate resulting in greater sensitivity. (randoxonlinestore.com)
  • Ectopic expression (CKBE) of the B (brain) type of creatine kinase (CK-BB) in red cells and platelets is a rare, benign anomaly detected during a newborn screening program for Duchenne muscular dystrophy. (wikipedia.org)
  • This forms the basis for the measurement of biochemical markers of myocardial injury in blood. (com.pk)
  • Creatine Kinase Isoenzymes in Cerebrospinal Fluid in a Case of Brain Damage. (aaltoscientific.com)
  • Cerebrospinal fluid enzymology: creatine kinase, lactate dehydrogenase activity and isozyme pattern as a brain damage index. (docphin.com)
  • Blood serum and cerebrospinal fluid from a 4.5-year-old girl suffering from convulsive episodes of toxic origin were investigated for lactate dehydrogenase (LDH) and creatine kinase (CK) activities. (docphin.com)
  • Diagnostic value of creatine kinase activity in canine cerebrospinal fluid. (docksci.com)
  • Article Diagnostic value of creatine kinase activity in canine cerebrospinal fluid Alexandra Ferreira Abstract - This study aimed to determine whether creatine kinase (CK) activity in cerebrospinal fluid (CSF) has diagnostic value for various groups of neurological conditions or for different anatomical areas of the nervous system (NS). (docksci.com)
  • Crystal structure of human brain-type creatine kinase with ADP and creatine. (wikipedia.org)
  • A KLH conjugated synthetic peptide selected within aa 200~300 of human Creatine Kinase BB. (abcam.com)
  • This includes physical injury 3,4,5 , various types of cancer 6,7 and neurodegenerative diseases such as Alzheimer's disease 8 , CJD 9 (the human form of cow disease) and a host of others 10 . (aaltoscientific.com)
  • A full-length human creatine kinase B (B-CK) cDNA was used to produce a recombinant baculovirus (AcDZ1-BCK). (semanticscholar.org)
  • A synthetic peptide corresponding to residues in Human Creatine Kinase BB. (abcam.com)
  • Creatine is a substance that is naturally produced by the human body from amino acids. (kidneypedia.net)
  • Vitro CPK (MB) reagent is an immunoinhibition assay intended for the in vitro quantitative determination of Creatine Kinase (CK-MB) in human serum and plasma. (vitroscient.com)
  • CK1 recognises human creatine kinase MB isoenzyme. (abcam.cn)
  • hCK-MB - 100% hCK-BB - 182% hCK-MM - 7.3% Affinity constant: 2x10 9 l/mol human CK-MB. (abcam.cn)
  • NH2-terminal amino acid sequence determinations show considerable sequence homology between human MM, Torpedo electric organ, chicken MM, and porcine MM creatine kinase. (mysciencework.com)
  • Purification and localization of brain-type creatine kinase in sodium chloride transporting epithelia of the spiny dogfish, Squalus acanthias. (semanticscholar.org)
  • Localization of creatine kinase isoenzymes in myofibrils. (rupress.org)
  • Small volumes of CK-BB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. (mybiosource.com)
  • MBS706326 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the creatine kinase BB isoenzyme (CK-BB) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • The ELISA Kit is designed to detect native, not recombinant, CK-BB. (mybiosource.com)
  • abstract = "Brain creatine kinase (CK)-catalyzed phosphorus flux from phosphocreatine (PC) to ATP was measured in vivo in young adult mice made reversibly hypoxic by injection of cyanide. (elsevier.com)
  • 0.05) restored all deranged biochemical parameters (creatine kinase, aspartate amino transferase, alanine amino transferase, alkaline phosphatase, urea, uric acid, bilirubin, MDA, lipid profile as well as superoxide dismutase and catalase) in a dose-dependent manner. (springeropen.com)
  • Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. (creative-enzymes.com)
  • Brain Creatine Kinase in Blood After Acute Brain Injury. (aaltoscientific.com)
  • High creatine kinase can also be a sign of acute renal failure. (kidneypedia.net)
  • Because of the illnesses it can indicate, a test for creatine kinase is indicated whenever a patient suffers from symptoms of heart attack, acute renal failure, or muscular breakdown. (kidneypedia.net)
  • Creatine Kinase Isoenzyme MB (CK-MB) in Acute Coronary Ischemia. (aaltoscientific.com)
  • Masticatory myositis can be divided into an acute and chronic form. (drjwv.com)
  • It must be remembered that one acute attack can lead immediately into the chronic form of the disease, although, more often than not, multiple, recurrent acute attacks are necessary. (drjwv.com)
  • Symptoms of the acute form involve the the swollen, firm and painful temporalis and masseter muscles. (drjwv.com)
  • some of which are shared with other forms of brain trauma such as acute brain injury and other neuropsychiatric disorders such as post-traumatic stress disorder. (frontiersin.org)
  • HLA-Dr-, CD33+, CD56+, CD16- myeloid/natural killer cell acute leukemia: A previously unrecognized form of acute leukemia potentially misdiagnosed as French- American British acute myeloid leukemia-M3. (openwetware.org)
  • Increased Serum Creatine Kinase BB and Neuron Specific Enolase Following Head Injury Indicates Brain Damage. (aaltoscientific.com)
  • Serum Creatine Kinase Isoenzyme BB as an indicator of Active Metastatic Disease. (aaltoscientific.com)
  • The Diagnostic and Prognostic Value of Pretreatment Serum Creatine Kinase BB Levels in Patients with Neroblastoma. (aaltoscientific.com)
  • The normal range of serum creatine kinase is between 10 and 120 micrograms per liter of blood. (kidneypedia.net)
  • When serum creatine kinase is elevated above the normal range, this can indicate any of several conditions. (kidneypedia.net)
  • 3. Smith, A. Diagnostic Value of Serum Creatine Kinase in a Coronary-Care Unit. (aaltoscientific.com)
  • https://doi.org/10.1371/journal.pone.0218329 decreased exercise capacity in treadmill and inverted hanging tests with higher levels of serum creatine kinase activities compared with those in WT mice. (deepdyve.com)
  • Other isoenzymes include CK MM and CK BB. (1mg.com)
  • The determination of CK isoenzymes in 5 brain regions of 2 dogs showed CK-BB, CK-MM, and CK-MB fractions of 95.8% to 98.2%, 1.2% to 3%, and 0.2% to 0.8%, respectively, of the total CK activity (2). (docksci.com)
  • The CK-MB Reagent is for the quantitative determination of the isoenzyme Creatine Kinase-MB (CK-MB) in serum on the Tokyo Boeki Medisys Inc. Biolis 24i analyzer. (pointescientific.com)
  • For the quantitative in vitro determination of Creatine Kinase in serum or plasma. (randoxonlinestore.com)
  • Determination of Creatine Kinase Isoenzyme MB (CK-MB): Comparison of Methods and Clinical Evaluation. (aaltoscientific.com)
  • In clinical diagnostic testing, creatine kinase levels in the blood are measured as part diagnosis of a number of illnesses. (kidneypedia.net)
  • Examples of the diagnostic use of isoenzymes are the study of Lactate Dehydrogenase and Creatine Kinase . (golifescience.com)
  • CreatineKinase BB (CK-BB) Isoenzyme Recombinant. (creative-diagnostics.com)
  • Gel filtration chromatography also showed that the recombinant enzyme existed both as homodimer as well as homotetramer with the dimer being the more abundant form. (thefreedictionary.com)
  • Creatine kinase (CK) activity was lower in Duchenne muscular dystrophy cultures and the isoenzyme distribution indicated MB-CK was significantly lower, while BB-CK was significantly higher in later Duchenne muscular dystrophy cultures. (elsevier.com)
  • This article is about the screening of 528,410, mostly 4-6-week-old, boys in Germany between 1977 and 2011 for high levels of creatine kinase (CK) to identify those with Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD). (cdc.gov)
  • Full length Creatine Kinase BBisoenzyme with amino acid sequence identical to the native enzyme. (creative-diagnostics.com)
  • Isoenzymes that catalyze the same physiologic reaction may also appear in different forms in different animal species. (nurseslearning.com)
  • Determining which isoenzyme (specific form of CK) is elevated will help determine which tissue has been damaged. (medical-labs.net)
  • In heart failure, changes in contributors to the passive properties of the ventricle, such as titin and collagen, and loss of the metabolic enzyme creatine kinase, increase resistance to filling resulting in diastolic dysfunction. (bris.ac.uk)
  • It has also been reported that in humans CSF CK-BB has a prognostic value in subclinical brain trauma (5) and recovery after cardiac arrest (6). (docksci.com)
  • Robbins says that troponin is the best overall but creatine kinase MB is also good. (pathologystudent.com)