A species of gram-negative bacteria that grows preferentially in the vacuoles of the host cell. It is the etiological agent of Q FEVER.
A genus of gram-negative, rod-shaped bacteria that is widely distributed in TICKS and various mammals throughout the world. Infection with this genus is particularly prevalent in CATTLE; SHEEP; and GOATS.
The phosphoprotein encoded by the BRCA1 gene (GENE, BRCA1). In normal cells the BRCA1 protein is localized in the nucleus, whereas in the majority of breast cancer cell lines and in malignant pleural effusions from breast cancer patients, it is localized mainly in the cytoplasm. (Science 1995;270(5237):713,789-91)
A large, nuclear protein, encoded by the BRCA2 gene (GENE, BRCA2). Mutations in this gene predispose humans to breast and ovarian cancer. The BRCA2 protein is an essential component of DNA repair pathways, suppressing the formation of gross chromosomal rearrangements. (from Genes Dev. 2000;14(11):1400-6)
Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.
A nucleocytoplasmic transport protein that binds to ALPHA KARYOPHERINS and RAN GTP BINDING PROTEIN inside the CELL NUCLEUS and participates in their export into CYTOPLASM. It is also associated with the regulation of APOPTOSIS and microtubule assembly.
An acute infectious disease caused by COXIELLA BURNETII. It is characterized by a sudden onset of FEVER; HEADACHE; malaise; and weakness. In humans, it is commonly contracted by inhalation of infected dusts derived from infected domestic animals (ANIMALS, DOMESTIC).
An enzyme of the transferase class that catalyzes the reaction 5,10-methylenetetrahydrofolate and dUMP to dihydrofolate and dTMP in the synthesis of thymidine triphosphate. (From Dorland, 27th ed) EC 2.1.1.45.
Uracil nucleotides which contain deoxyribose as the sugar moiety.
5-Fluoro-2'-deoxyuridylate. An inhibitor of thymidylate synthetase. Formed from 5-fluorouracil or 5-fluorodeoxyuridine.
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Inflammation of the ENDOCARDIUM caused by BACTERIA that entered the bloodstream. The strains of bacteria vary with predisposing factors, such as CONGENITAL HEART DEFECTS; HEART VALVE DISEASES; HEART VALVE PROSTHESIS IMPLANTATION; or intravenous drug use.
Diseases of the domestic or wild goat of the genus Capra.
A highly reactive aldehyde gas formed by oxidation or incomplete combustion of hydrocarbons. In solution, it has a wide range of uses: in the manufacture of resins and textiles, as a disinfectant, and as a laboratory fixative or preservative. Formaldehyde solution (formalin) is considered a hazardous compound, and its vapor toxic. (From Reynolds, Martindale The Extra Pharmacopoeia, 30th ed, p717)
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
An adrenergic beta-2 agonist used to control PREMATURE LABOR.
A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).
An acute, diffuse, and suppurative inflammation of loose connective tissue, particularly the deep subcutaneous tissues, and sometimes muscle, which is most commonly seen as a result of infection of a wound, ulcer, or other skin lesions.
A tri-hydroxy cyclohexene carboxylic acid important in biosynthesis of so many compounds that the shikimate pathway is named after it.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
EPIDEMIOLOGIC STUDIES based on the detection through serological testing of characteristic change in the serum level of specific ANTIBODIES. Latent subclinical infections and carrier states can thus be detected in addition to clinically overt cases.
The branch of medicine concerned with diseases, mainly of parasitic origin, common in tropical and subtropical regions.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
A genus of gram-negative, aerobic, rod-shaped bacteria often surrounded by a protein microcapsular layer and slime layer. The natural cycle of its organisms generally involves a vertebrate and an invertebrate host. Species of the genus are the etiological agents of human diseases, such as typhus.
A major protein fraction of milk obtained from the WHEY.
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.

Predominant immunoglobulin A response to phase II antigen of Coxiella burnetii in acute Q fever. (1/454)

Diagnosis of acute Q fever is usually confirmed by serology, on the basis of anti-phase II antigen immunoglobulin M (IgM) titers of >/=1:50 and IgG titers of >/=1:200. Phase I antibodies, especially IgG and IgA, are predominant in chronic forms of the disease. However, between January 1982 and June 1998, we observed anti-phase II antigen IgA titers of >/=1:200 as the sole or main antibody response in 10 of 1,034 (0.96%) patients with acute Q fever for whom information was available. In order to determine whether specific epidemiological or clinical factors were associated with these serological profiles, we conducted a retrospective case-control study that included completion of a standardized questionnaire, which was given to 40 matched controls who also suffered from acute Q fever. The mean age of patients with elevated phase II IgA titers was significantly higher than that usually observed for patients with acute Q fever (P = 0.026); the patients were also more likely than controls to live in rural areas (P = 0.026) and to have increased levels of transaminase in blood (P = 0.03). Elevated IgA titers are usually associated with chronic Q fever and are directed mainly at phase I antigens. Although the significance of our findings is unexplained, we herein emphasize the fact that IgA antibodies are not specific for chronic forms of Q fever and that they may occasionally be observed in patients with acute disease. Moreover, as such antibody profiles may not be determined by most laboratories, which test only for total antibody titers to phase I and II antigens, the three isotype-specific Ig titers should be determined as the first step in diagnosing Q fever.  (+info)

Functional analysis of the active partition region of the Coxiella burnetii plasmid QpH1. (2/454)

The partition region qsopAB of the Coxiella burnetii plasmid QpH1 was analyzed. Locus qsopA alone appears to fulfill the partitioning function; QsopA represses its own promoter 17-fold. Two partition-associated incompatibility sites were identified: incA in a 200-bp region covering the qsopA promoter and incB in the qsopB locus.  (+info)

Q fever in Bulgaria and Slovakia. (3/454)

As a result of dramatic political and economic changes in the beginning of the 1990s, Q-fever epidemiology in Bulgaria has changed. The number of goats almost tripled; contact between goat owners (and their families) and goats, as well as goats and other animals, increased; consumption of raw goat milk and its products increased; and goats replaced cattle and sheep as the main source of human Coxiella burnetii infections. Hundreds of overt, serologically confirmed human cases of acute Q fever have occurred. Chronic forms of Q fever manifesting as endocarditis were also observed. In contrast, in Slovakia, Q fever does not pose a serious public health problem, and the chronic form of infection has not been found either in follow-ups of a Q-fever epidemic connected with goats imported from Bulgaria and other previous Q-fever outbreaks or in a serologic survey. Serologic diagnosis as well as control and prevention of Q fever are discussed.  (+info)

Coxiella burnetii infection increases transferrin receptors on J774A. 1 cells. (4/454)

Inoculation with viable, but not inactivated, Coxiella burnetii resulted in the increased expression of transferrin receptors (TfR) in the murine macrophage-like cell line J774A.1. This upregulation was evident in immunoblots as early as 6 h postinfection, with TfR levels continuing to increase through the first 24 h of infection. Fluorescent labeling revealed that TfR upregulation occurred throughout infected monolayers, eliminating the possibility that it reflected a response by a minor subset of host cells. In addition, TfR trafficking did not appear to be affected by C. burnetii infection. Consistent with the increase in TfRs, inoculation with viable C. burnetii resulted in a 2.5-fold increase in total cellular iron by 12 h postinoculation. Our further findings that the chelation of intracellular iron arrests C. burnetii replication and that C. burnetii metabolic activities in vitro are affected by iron concentration suggest that TfR upregulation is a salient factor in C. burnetii infection, and we speculate that it may represent a significant virulence mechanism.  (+info)

Long term vascular complications of Coxiella burnetii infection in Switzerland: cohort study. (5/454)

OBJECTIVE: To evaluate the range of long term vascular manifestations of Coxiella burnetii infection. DESIGN: Cohort study in Switzerland of people affected in 1983 by the largest reported outbreak of Q fever and who were followed up 12 years later. Follow up information about possible vascular disease and endocarditis was obtained through a mailed questionnaire and death certificates. SETTING: Val de Bagnes, a rural Alpine valley in Switzerland. PARTICIPANTS: 2044 (87%) of 2355 people who had serum testing for Coxiella burnetii infection in 1983: 1247 were classed as not having been infected, 411 were classed as having been acutely infected, and 386 were classed as having been infected before 1983. MAIN OUTCOME MEASURES: Relative risk controlled for age and sex and 12 year risk of vascular diseases and endocarditis among infected participants as compared with those who had never been infected. RESULTS: The 12 year risk of endocarditis or venous thromboembolic disease was not increased among those who had been acutely infected. The 12 year risk of arterial disease was significantly higher among those who had been acutely infected (7%) as compared with those who had never been infected (4%) (relative risk 2.2, 95% confidence interval 1.4 to 3.6). Specifically, there was an increased risk of developing a cerebrovascular accident (relative risk 3.7, 1.6 to 8.4) and cardiac ischaemia (relative risk 1.9, 1.04 to 3.4). 12 year mortality was significantly higher among the 411 people who had been acutely infected in 1983 (9.7%; age adjusted relative risk 1.8, 1.2 to 2.6) when compared with the 1247 participants who had remained serologically negative in 1983 (7.0%). CONCLUSIONS: Coxiella burnetii infection may cause long term complications including vascular disease.  (+info)

Short report: prevalence of antibodies against spotted fever, murine typhus, and Q fever rickettsiae in humans living in Zambia. (6/454)

The causative agents of rickettsial diseases (Rickettsia conorii, R. typhi, and Coxiella burnetii) have been reported throughout the African continent. However, there have been no reports on epidemiologic surveys of these infections in Zambia. This study was designed to clarify the prevalence of three rickettsioses in 377 humans in Zambia. The seroprevalence of antibodies against R. conorii, R. typhi, and C. burnetii was 16.7%, 5.0%, and 8.2%, respectively. The rates of antibody positivity against R. conorii and C. burnetii were higher in the eastern (23.1% and 11.8%) and western (16.8% and 7.4%) areas of Zambia than in the northern (3.0% and 3.0%) area of this country. There was little difference among the three areas in the distribution of antibodies against R. typhi. Since cattle breeding is more extensive in the western and eastern areas than in the northern area, it is thought that cattle-breeding areas are foci of R. conorii and C. burnetii infections in Zambia.  (+info)

Coxiella burnetii pericarditis: report of 15 cases and review. (7/454)

Q fever is characterized by its clinical polymorphism, and pericarditis associated with Q fever has occasionally been described. Herein we report 15 cases of Coxiella burnetii pericarditis, 9 from our data bank and 6 encountered within the past 12 months. Three patients presented with life-threatening tamponade. We compare our cases with the 18 previously reported and with 60 Q fever-matched controls at our center. This study showed that Q fever pericarditis can present as acute as well as chronic disease; we describe relapse after 6 months in association with a serological profile compatible with the chronic form of disease (phase I C. burnetii IgG titer of > or = 800). Discriminant factors among patients and controls are age of > 52 years (adjusted odds ratio [OR], 5.66), the occurrence of general symptoms such as arthralgias or myalgias (adjusted OR, 6.54), and a normal erythrocyte sedimentation rate (adjusted OR, 16.37). No specific symptoms or underlying cardiac predispositions are observed.  (+info)

Q fever. (8/454)

Q fever is a zoonosis with a worldwide distribution with the exception of New Zealand. The disease is caused by Coxiella burnetii, a strictly intracellular, gram-negative bacterium. Many species of mammals, birds, and ticks are reservoirs of C. burnetii in nature. C. burnetii infection is most often latent in animals, with persistent shedding of bacteria into the environment. However, in females intermittent high-level shedding occurs at the time of parturition, with millions of bacteria being released per gram of placenta. Humans are usually infected by contaminated aerosols from domestic animals, particularly after contact with parturient females and their birth products. Although often asymptomatic, Q fever may manifest in humans as an acute disease (mainly as a self-limited febrile illness, pneumonia, or hepatitis) or as a chronic disease (mainly endocarditis), especially in patients with previous valvulopathy and to a lesser extent in immunocompromised hosts and in pregnant women. Specific diagnosis of Q fever remains based upon serology. Immunoglobulin M (IgM) and IgG antiphase II antibodies are detected 2 to 3 weeks after infection with C. burnetii, whereas the presence of IgG antiphase I C. burnetii antibodies at titers of >/=1:800 by microimmunofluorescence is indicative of chronic Q fever. The tetracyclines are still considered the mainstay of antibiotic therapy of acute Q fever, whereas antibiotic combinations administered over prolonged periods are necessary to prevent relapses in Q fever endocarditis patients. Although the protective role of Q fever vaccination with whole-cell extracts has been established, the population which should be primarily vaccinated remains to be clearly identified. Vaccination should probably be considered in the population at high risk for Q fever endocarditis.  (+info)

CBSRP : Coxiella burnetii, the causative agent of Q fever, is a small obligately intracellular bacterium, which is associated with animals. It is acquired through aerosol exposure and generally causes mild respiratory disease. A small number of acute cases advance to a chronic infection, which typically manifests as endocarditis. Left untreated, Q fever endocarditis may be fatal. Serologic and histopathologic studies may be nonspecific and subjective, respectively, limiting usefulness for patient diagnosis.   Evaluation of infected tissue, blood, or serum using PCR may be a useful tool for diagnosing some cases of Coxiella burnetii infection. Mayo Medical Laboratories has developed a real-time PCR test that rapidly detects Coxiella burnetii DNA in clinical specimens by targeting a sequence of the shikimate dehydrogenase gene (aroE) unique to Coxiella burnetii.
TY - JOUR. T1 - Dynamics of relationship between the presence of Coxiella burnetii DNA, antibodies, and intrinsic variables in cow milk and bulk tank milk from Danish dairy cattle. AU - Angen,Øystein. AU - Ståhl,Marie. AU - Agerholm,J. S.. AU - Christoffersen,Anna-Bodil. AU - Agger,J. F.. N1 - Copyright 2011 American Dairy Science Association.. PY - 2011. Y1 - 2011. N2 - Milk samples of 12 Danish dairy herds were collected 3 times during an 11-mo period and tested for Coxiella burnetii DNA by real-time PCR, detecting the IS1111 element, and for the presence of antibodies against the bacterium by ELISA. On average, 25% of 1,514 samples were seropositive and 32% were positive for C. burnetii DNA. Among the 485 DNA-positive samples, quantification cycle values ranging from 15.8 to 37.8 were found. Test sensitivity did not increase after DNA extraction from the cream fraction compared with full milk. The relationship between antibody levels and bacterial shedding was investigated among 166 cows ...
Coxiella burnetii, the etiological agent of Q fever, is a Gram-negative bacterium transmitted to humans by inhalation of contaminated aerosols. Acute Q fever is often self-limiting, presenting as a febrile illness that can result in atypical pneumonia. In some cases, Q fever becomes chronic, leading to endocarditis that can be life threatening. The formalin-... ...
BACKGROUND: Prior to the 2007-2010 Q fever epidemic in the Netherlands, the seroprevalence of antibodies against Coxiella burnetii in the general population was 1.5%, which is low compared to other countries. We aimed to determine the seroprevalence after the Q fever epidemic among people living in the affected area, compare ... read more the seroprevalence with the incidence of Q fever notifications during the 2007-2010 Q fever epidemic, and to identify farm exposures associated with having antibodies against C. burnetii. METHODS: During the period March 2014-February 2015, residents aged 18-70 years from two provinces were invited by general practitioners to complete a questionnaire on their symptoms and personal characteristics and to submit a blood sample. We used the mandatory provincial database of livestock licences to calculate distance to farms/farm animals for each participant. To compare ELISA-positive participants for C. burnetii antibodies with those who were negative, we calculated ...
Coxiella burnetii is an intracellular bacterial pathogen that causes Q fever. Infected pregnant goats are a major source of human infection. However, the tissue dissemination and excretion pathway of the pathogen in goats are still poorly understood. To better understand Q fever pathogenesis, we inoculated groups of pregnant goats via the intranasal route with a recent Dutch outbreak C. burnetii isolate. Tissue dissemination and excretion of the pathogen were followed for up to 95 days after parturition. Goats were successfully infected via the intranasal route. PCR and immunohistochemistry showed strong tropism of C. burnetii towards the placenta at two to four weeks after inoculation. Bacterial replication seemed to occur predominantly in the trophoblasts of the placenta and not in other organs of goats and kids. The amount of C. burnetii DNA in the organs of goats and kids increased towards parturition. After parturition it decreased to undetectable levels: after 81 days post-parturition in goats and
Coxiella burnetii is the causative agent of human Q fever, eliciting symptoms that range from acute fever and fatigue to chronic fatal endocarditis. C. burnetii is a Gram-negative intracellular bacterium that replicates within an acidic lysosome-like parasitophorous vacuole (PV) in human macrophages. During intracellular growth, C. burnetii delivers bacterial proteins directly into the host cytoplasm using a Dot/Icm type IV secretion system (T4SS). Multiple T4SS effectors localize to and/or disrupt the endoplasmic reticulum (ER) and secretory transport, but their role in infection is unknown. During microbial infection, unfolded nascent proteins may exceed the folding capacity of the ER, activating the unfolded protein response (UPR) and restoring the ER to its normal physiological state. A subset of intracellular pathogens manipulates the UPR to promote survival and replication in host cells. In this study, we investigated the impact of C. burnetii infection on activation of the three arms of ...
Summary Experimental Coxiella burnetii infection in deer mice, desert wood rats, Montane meadow mice, Ord kangaroo rats, pinyon mice, and laboratory white mice was studied, employing three criteria: 28-day Phase II complement fixing antibody responses, splenomegaly, and tissue infection. Infection in guinea pigs was determined by fever response in addition to the above. All criteria gave similar ID50 values with the exception of splenomegaly, which was absent in some animal species. All tested animals were shown to be readily susceptible to intraperitoneal Q fever infection, the laboratory animals being more susceptible than the wild rodents. Disease manifestations were mild in all animals.
Abstract. Transmission by the oral route of Coxiella burnetii is controversial. Our objective was to evaluate dairy products in the transmission of Q fever. Pasteurized, unpasteurized, and thermized dairy products were tested for C. burnetii by using a quantitative polymerase chain reaction specific for IS1111 and IS30A spacers, culturing in human embryonic lung fibroblasts cells, and inoculation into BALB/c mice. We tested 201 products and C. burnetii was identified in 64%. Cow milk origin products were more frequently positive than goat or ewe products (P = 0.006 and P = 0.0001, respectively), and industrial food was more frequently positive than artisanal food (P < 0.0001). Food made from unpasteurized milk contained higher bacteria concentrations than food made from pasteurized milk (P = 0.02). All cultures were negative and mice did not show signs of illness. Farm animals are highly infected in France but consumption of cheese and yogurt does not seem to pose a public health risk for
The objective of this study was to investigate the prevalence of Coxiella burnetii in two domestic ruminant species (cattle and sheep) and the western grey kangaroo (Macropus fuliginosus) in Western Australia (WA). The IDEXX CHEKiT Q Fever ELISA and CFT were used to test sera from 50 sheep and 329 head of cattle for anti- C. burnetii antibodies and 343 kangaroo sera were tested using an indirect ELISA developed specifically for this study. Faecal or urine samples collected from the same animals were tested with two PCR assays to identify active shedding of C. burnetii in excreta. Only two of the 379 ruminant sera had detectable levels of anti- C. burnetii antibodies according to the ELISA while the CFT did not detect any positive samples. In contrast 115 of the 343 western grey kangaroo serum samples were positive when tested with the antibody-ELISA. The first qPCR assay, targeting the IS1111a element, identified 41 of 379 ruminant and 42 of 343 kangaroo DNA samples as positive for C. burnetii ...
Coxiella burnetii symptoms, causes, diagnosis, and treatment information for Coxiella burnetii (Q fever) with alternative diagnoses, full-text book chapters, misdiagnosis, research treatments, prevention, and prognosis.
Coxiella burnetii, a gram-negative obligate intracellular bacterium, causes human Q fever and is considered a potential agent of bioterrorism. Distinct genomic groups of C. burnetii are revealed by restriction fragment-length polymorphisms (RFLP). Here we comprehensively define the genetic diversity …
Q fever caused by Coxiella burnetii is transmitted to humans by inhalation of aerosols from animal birth products. Q fever in pregnancy is suspected to be a potential cause of fetal and maternal morbidity and fetal mortality but the pathogenesis is poorly understood, and even in Q fever endemic areas, the magnitude of a potential association is not established. We aimed to examine if presence of antibodies to C. burnetii during pregnancy or seroconversion were associated with adverse pregnancy outcomes. The Danish National Birth Cohort collected blood samples and interview data from 100,418 pregnant women (1996-2002). We sampled 397 pregnant women with occupational or domestic exposure to cattle or sheep and a random sample of 459 women with no animal exposure. Outcome measures were spontaneous abortion, preterm birth, birth weight and Small for Gestational Age (SGA). Blood samples collected in pregnancy were screened for antibodies against C. burnetii by enzyme-linked immunosorbent assay (ELISA).
Q fever caused by Coxiella burnetii is transmitted to humans by inhalation of aerosols from animal birth products. Q fever in pregnancy is suspected to be a potential cause of fetal and maternal morbidity and fetal mortality but the pathogenesis is poorly understood, and even in Q fever endemic areas, the magnitude of a potential association is not established.We aimed to examine if presence of antibodies to C. burnetii during pregnancy or seroconversion were associated with adverse pregnancy outcomes ...
The intervening sequence (IVS) of Coxiella burnetii, the agent of Q fever, is a 428-nt selfish genetic element located in helix 45 of the precursor 23S rRNA. The IVS element, in turn, contains an ORF that encodes a hypothetical ribosomal S23 protein (S23p). Although S23p can be synthesized in vitro in the presence of an engineered E. coli promoter and ribosome binding site, results suggest that the protein is not synthesized in vivo. In spite of a high degree of IVS conservation among different strains of C. burnetii, the region immediately upstream of the S23p start codon is prone to change, and the S23p-encoding ORF is evidently undergoing reductive evolution. We determined that IVS excision from 23S rRNA was mediated by RNase III, and IVS RNA was rapidly degraded, thereafter. Levels of the resulting 23S rRNA fragments that flank the IVS, F1 (~1.2 kb) and F2 (~1.7 kb), were quantified over C. burnetiis logarithmic growth phase (1-5d). Results showed that 23S F1 quantities were consistently higher
Genetic variations in innate immunity genes affect response to Coxiella burnetii and are associated with susceptibility to chronic Q ...
McCaul T.F., Williams J.C. and Thompson H.A. (1991) Electron microscopy of Coxiella burnetii in tissue culture. Induction of cell types as products of developmental cycle.. Acta Virologica, 35 6: 545-556. ...
There are comments on PubPeer for publication: Activation of protein tyrosine kinases by Coxiella burnetii: role in actin cytoskeleton reorganization and bacterial phagocytosis (2001)
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Characterization of the GDP-D-Mannose Biosynthesis Pathway in Coxiella burnetii: The Initial Steps for GDP-β-D-Virenose Biosynthesis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
This is a collaborative project between veterinary and medical colleagues from NSW and Queensland to determine the importance of Q fever in the veterinary community as well as the broader Australian population. It brings together veterinarians from the University of Sydney (Assoc. Prof. Jacqui Norris, Dr Katrina Bosward, Dr Navneet Dhand), Charles Sturt University (Dr Jane Heller), the University of Queensland (Dr Rowland Cobbold) and James Cook University as well as medical colleagues from Sydney (Dr Nicholas Wood, Professor Peter McIntyre, Dr Heather Gidding, Professor Dominic Dwyer), the New England/Hunter region (Prof David Durrheim, Assoc. Prof. Stephen Graves), Brisbane (Professor Michael Nissen, Mrs Sarah Tozer, Dr Theo Sloots) and north east Queensland (Townsville, Dr Peter Massey).. Q fever is a ubiquitous zoonotic disease of worldwide importance caused by the bacterium Coxiella burnetii. It is classified as a category B bioterrorism agent and is a significant cause of acute and chronic ...
Q fever is caused by the bacterium Coxiella burnetii and has both acute and chronic forms. The acute disease is a febrile illness often with headache and myalgia that can be self-limiting, whereas the chronic disease typically presents as endocarditis and can be life threatening. The normal therapy for the acute disease is a 2 week course of doxycycline, whereas chronic disease requires 18-24 months of doxycycline in combination with hydroxychloroquine. Alternative treatments are used for pregnant women, young children and those who cannot tolerate doxycycline. Doxycycline resistance is rare, but has been reported. Co-trimoxazole is a currently recommended alternative treatment, but quinolones, rifampin and newer macrolides may also provide some benefit ...
En citoloxía denomínase fagosoma un vacúolo formado arredor dunha partícula que foi absorbida pola célula por fagocitose. Son típicos da célula animal. O vacúolo fórmase pola fusión da membrana celular arredor da partícula. O fagosoma é un compartimento celular no cal poden dixerirse e destruírse microorganismos patóxenos. Os fagosomas fusiónanse con lisosomas no seu proceso de maduración, formando fagolisosomas. Algunhas bacterias patóxenas que chegan ao interior da célula ao seren fagocitadas, poden reproducirse no interior do fagosoma ou mesmo no interior do fagolisosoma, como é o caso da bacteria Coxiella burnetii.[1] Outras pasan ao citoplasma antes de que o fagosoma se fusione co lisosoma, como sucede con Rickettsia[2]. Moitas micobacterias, entre as que se inclúe Mycobacterium tuberculosis[3][4] e a Mycobacteria avium paratuberculosis[5], manipula ao seu hospedador, un macrófago, para impedir que os lisosomas cargados de ácido nitroso se fusionen cos fagosomas e ...
The scope and overall interpretation of our report on the re-evaluation of the fixed, paraffin-embedded tissue samples taken at patient BIs autopsy are necessarily limited by the absence of diagnostic results of any serological or other tests for candidate infective organisms at the time of the viral encephalitis in 1986 (see review [17]). Nevertheless, taken together, the extended laboratory tests with C.b. specific monoclonal antibodies and PCR (COM1 and IS1111a genes) on a range of post mortem specimens suggest that the most compelling and coherent explanation of BIs illness from 1986 to 1996, is one of a severe attack of primary Q fever and a subsequent multisystem organ dysfunction with dissemination of the coxiella throughout the body, ending in 1996 with cardiac and cerebral dysfunction i.e., a complex, severe idiopathic illness labelled descriptively at the time as post (viral) infection fatigue syndrome (PIFS).. An epidemiological and clinical association between ...
Catalyzes the reductive methylation of 2-deoxyuridine-5-monophosphate (dUMP) to 2-deoxythymidine-5-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.
The focus of our research is to understand the molecular and cellular events that enable microbial pathogens to evade host defense mechanisms. In particular, we are interested in how bacteria that replicate inside mammalian cells create specialized vacuoles that support pathogen replication. We have been using Legionella pneumophila and Coxiella burnetii as model pathogens to study this process. We have been characterizing a type IV secretion system called Dot/Icm that delivers bacterial effector proteins into the eukaryotic host cell cytosol. The goals of this research are to determine the mechanism by which these bacterial effector proteins regulate phagosome maturation, modulate host immunity, and subvert eukaryotic cell functions.. Specialized Terms: Molecular; Cellular; Microbial pathogens; Bacteria; Vesicular transport; Legionella pneumophila; Coxiella burnetii; Macrophages; Protozoan; ...
AmJTrop Med Hyg 2009, 81:67-74. 22. Willems H, Thiele D, Frolich-Ritter R, Krauss H: Detection of Coxiella burnetii in cows check details milk using the polymerase chain reaction (PCR). Zentralbl Vet B 1994, 41:580-587. 23. Berri M, Laroucau K, Rodolakis A: The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction. Vet Microbiol 2000, 72:285-293.PubMedCrossRef 24. Barandika JF, Hurtado A, García-Esteban C, Gil H, Escudero R, Barral M, Jado I, Juste RA, Anda P, García-Pérez AL: Tick-borne zoonotic bacteria in wild and domestic small mammals in northern Spain. Appl Environ Microbiol. 2007, 73:6166-6171.PubMedCrossRef 25. Jado I, Escudero R, Gil H, Jiménez-Alonso MI, Sousa R, García-Pérez AL, Rodríguez-Vargas M, Lobo B, Anda P: Molecular method for identification of Rickettsia species in clinical and environmental samples. J Clin Microbiol 2006, 44:4572-4576.PubMedCrossRef 26. Montejo-Baranda M, ...
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Nucleotide excision repair (NER) is a mechanism to recognize and repair bulky DNA damage caused by compounds, environmental carcinogens, and exposure to UV-light. In humans hereditary defects in the NER pathway are linked to at least three diseases: xeroderma pigmentosum (XP), Cockayne syndrome (CS), and trichothiodystrophy (TTD). The repair of damaged DNA involves at least 30 polypeptides within two different sub-pathways of NER known as transcription-coupled repair (TCR-NER) and global genome repair (GGR-NER). TCR refers to the expedited repair of lesions located in the actively transcribed strand of genes by RNA polymerase II (RNAP II). In GGR-NER the first step of damage recognition involves XPC-hHR23B complex together with XPE complex (in prokaryotes, uvrAB complex). The following steps of GGR-NER and TCR-NER are similar ...
Homologous recombination (HR) is essential for the accurate repair of DNA double-strand breaks (DSBs), potentially lethal lesions. HR takes place in the late S-G2 phase of the cell cycle and involves the generation of a single-stranded region of DNA, followed by strand invasion, formation of a Holliday junction, DNA synthesis using the intact strand as a template, branch migration and resolution. It is investigated that RecA/Rad51 family proteins play a central role. The breast cancer susceptibility protein Brca2 and the RecQ helicase BLM (Bloom syndrome mutated) are tumor suppressors that maintain genome integrity, at least in part, through HR ...
1. A 21-day whole body x-irradiation LD50 was determined for guinea pigs, white mice and deer mice. As measured by post-exposure deaths, guinea pigs were most susceptible (LD50: 163 r), white mice less susceptible (LD50: 431 r) and deer mice least susceptible (LD50: 588 r) to the irradiation. 2. In a pilot rickettsiae study, Coxiella burnetii was shown to persist for over 12 weeks in kidneys of guinea pigs and reproductive tract and kidneys of white mice. Fecal material and urine in guinea pigs, white mice and deer mice were infectious for three weeks. The organism persisted a total of six week in deer mouse spleen, kidneys and liver. 3. Whole body x-irradiation in dosages slightly less than or greater than the 21-day LD50 caused a reactivation of C. burnetii infection in guinea pigs, white mice, and deer mice infected three months previously. This reactivation was determined by demonstrating infectious quantities of rickettsiae in various tissues and in urine and feces in these animals, as ...
Vilibić-Čavlek, Tatjana and Kučinar, Jasmina and Kaić, Bernard and Vilibić, Maja and Pandak, Nenad and Barbić, Ljubo and Stevanović, Vladimir and Vraneš, Jasmina (2015) Epidemiology of hepatitis C in Croatia in the European context. World Journal of Gastroenterology, 21 (32). pp. 9476-93. ISSN 1007-9327 Kaić, Bernard (2012) Utjecaj medikamentnog liječenja na trajanje opsežnih lokalnih reakcija nakon primjene kombiniranih acelularnih cjepiva protiv pertusisa [Effect of medication on the duration of large local reactions following administration of combination acellular pertussis vaccines]. PhD thesis, Sveučilište u Zagrebu. Vilibić-Čavlek, Tatjana and Kučinar, Jasmina and Ljubin-Sternak, Sunčanica and Kolarić, Branko and Kaić, Bernard and Lazarić-Stefanović, Lorena and Hunjak, Blaženka and Mlinarić-Galinović, Gordana (2012) Prevalence of Coxiella burnetii antibodies among febrile patients in Croatia, 2008-2010. Vector Borne and Zoonotic Diseases, 12 (4). pp. 293-6. ISSN ...
CIDRAP News) The genetic blueprint of Coxiella burnetii, a category B bioterrorism agent that causes Q fever, has been decoded and analyzed, the National Institute of Allergy and Infectious Diseases (NIAID) announced this week. ...
A high risk for obstetric complications has been reported among women infected with Coxiella burnetii, the causative agent of Q fever, but recent studies have failed to confirm these findings. We reviewed national data collected in Denmark during 2007-2011 and found 19 pregnancies in 12 women during which the mother had a positive or equivocal test for antibodies to C. burnetii (IgM phase I and II titers >64, IgG phase I and II titers >128). Of these 12 women, 4 experienced obstetric complications (miscarriage, preterm delivery, infant small for gestational age, oligohydramnion, fetal growth restriction, or perinatal death); these complications occurred in 9 pregnancies (47% of the 19 total pregnancies identified). Our findings suggest an association between Q fever and adverse pregnancy outcomes, but complications were identified in only 9 pregnancies during the studys 5-year period, indicating that the overall risk is low ...
Dr. Malinda Fitzgerald, Professor of Biology and Alpha Chi faculty advisor, attended the National Alpha Chi Convention in Chicago in March with Johnnie Huddleston. Johnnie graduated with her BFA from CBU while going part time and working in the business office. She is interested in applying to Masters programs in digital media. Johnnie presented a talk on My Vision of Art and placed on the alternate list for the national scholarship to graduate school from AX. This is a very competitive scholarship and if anyone doesnt accept she is next on the list. Dr. Fitzgerald is serving as the president of Regional III for Alpha Chi and it has over 78 schools and is one of the largest regions. ***. This piece on the Science Fair is from Wendy Sumner-Winter, Sr. Director of External Affairs and Advancement for CBU. On Saturday, Feb. 28, students from high schools throughout the Memphis area gathered at Christian Brothers University (CBU) to participate in the Science Olympiad. This years Olympiad ...
The increases in MAP and HR recorded via telemetry during the first week after CBU are very similar to results published previously based on daily recordings with the dog resting quietly in a sling under laboratory conditions (28). The effects of CBU on PRA were also replicated; PRA was elevated on some days during the week following CBU but never suppressed below control levels. Furthermore, we also observed a significant increase in plasma NE in response to CBU. All of these responses are compatible with the hypothesis that CBU caused an increase in sympathetic outflow. However, contrary to our expectations, the initial increases in MAP, HR, and plasma NE were not sustained. Plasma NE and HR declined to control levels by the third week after CBU (Figs. 4 and 7). There was also a decline in MAP over the same time period, but MAP stabilized at a level that averaged 10 ± 3 mmHg above control during the third to fifth weeks after CBU (Fig. 2). There are a number of possibilities that could ...
Q-fever, a rare infection caused by the Coxiella burnetii organism, has been confirmed in workers at the Scotbeef Meat Processing Company in Bridge of Allan.
Time Course of the Levels of Antibodies to Coxiella burnetii and Detection of C. burnetii-DNA in Three Imported Cases of Acute Q ...
9. O. Duron, V. Noël, K. D. McCoy, M. Bonazzi, K. Sidi-Boumedine, O. Morel, F. Vavre, L. Zenner, E. Jourdain, P. Durand, C. Arnathau, F. Renaud, J.F. Trape, A. S. Biguezoton, J. Cremaschi, M. Dietrich, E. Léger, A. Appelgren, M. Dupraz, E. Gomez-Diaz, G. Diatta, G.K. Dayo, Hassane Adakal, S. Zoungrana, L. Vial and C. Chevillon. The Recent Evolution of a Maternally-Inherited Endosymbiont of Ticks Led to the Emergence of the Q Fever Pathogen, Coxiella burnetii. PLOS Pathogen. 2015. May 15. DOI:10.1371/journal.ppat. ...
9. O. Duron, V. Noël, K. D. McCoy, M. Bonazzi, K. Sidi-Boumedine, O. Morel, F. Vavre, L. Zenner, E. Jourdain, P. Durand, C. Arnathau, F. Renaud, J.F. Trape, A. S. Biguezoton, J. Cremaschi, M. Dietrich, E. Léger, A. Appelgren, M. Dupraz, E. Gomez-Diaz, G. Diatta, G.K. Dayo, Hassane Adakal, S. Zoungrana, L. Vial and C. Chevillon. The Recent Evolution of a Maternally-Inherited Endosymbiont of Ticks Led to the Emergence of the Q Fever Pathogen, Coxiella burnetii. PLOS Pathogen. 2015. May 15. DOI:10.1371/journal.ppat. ...
A febre Q é uma zoonose ubíqua provocada por uma rickettsia, Coxiella burnetii, ocorrendo como casos esporádicos em zonas de pastagem de ovinos e caprinos e podendo ser responsáveis por surtos humanos (1). O modo de transmissão predominante é a inalação de aerossóis infectados provenientes do meio ambiente (solo, palha, lã), contaminado após o período de parto do gado ovino, caprino ou bovino (1). Neste artigo, descrevemos um surto urbano de febre Q que ocorreu de Março a Maio de 1996, em Briançon, uma cidade de 12 000 habitantes, situada numa zona de pastagem dos Hautes Alpes.
Get info about CBU dialysis technician program, registering for required degree courses, and taking practice exams for free online. Seek out nationally-accredited nursing programs for the best training. Given a brief hospital internship or prior work experience, you can expect a great return on your education.
Information about CBU associates degrees online. Qualifications for technical jobs vary considerably. Learn about accredited degree programs, and certificate courses that you can take to advance your career.
For example, the mynavy is simply a rehashed version of A800 that you can buy from China retail shops (walk into a electronic centre) and you can see they sell it for about RM$400. If you can buy it from a Chinese shop, hnow much do you think you can buy at OEM volume? Proton is selling it for over $1,000. How much pocket change are they keeping? The maps that can originally be bought from TeleAtlas (very big mapping company) is under RM$50. The current maps powered by Mynavi is by TM. A GPs navigator is a fun system to use, assuming the map is good (which mynavi does not). You still need to provide additional services for a sophisticated consumer, includes ...
Int. J. Softw. Tools Technol. Transf., 18 (6), 581-586. Neveling K, Mensenkamp AR, Derks R, Kwint M, Ouchene H, Steehouwer M, van Lier B, Bosgoed E, Rikken A, Tychon M, Zafeiropoulou D, Castelein S, Hehir-Kwa J, Tjwan Thung D, Hofste T, Lelieveld SH, Bertens SM, Adan IB, Eijkelenboom A, Tops BB, Yntema H, Stokowy T, Knappskog PM, Høberg-Vetti H, Steen VM et al. (2016 ...
AP1_KLULA (P56095 ), ARGC_CHRVO (Q7NRT5 ), BAH_RHOER (Q8RSQ2 ), BTUB_PHOLL (Q7MYE3 ), CBID_PROMP (Q7V3N8 ), CHS1_CRYNH (O13356 ), CLS2_STAAC (Q5HEB2 ), CLS2_STAAR (Q6GEY7 ), CLS_STAAM (P63800 ), CLS_STAAN (P63801 ), CLS_STAAS (Q6G7M2 ), CLS_STAAW (P63802 ), COBT_DEIRA (Q9RYR8 ), DCL4_ORYSJ (A7LFZ6 ), DMXL1_HUMAN (Q9Y485 ), DMXL1_MOUSE (Q6PNC0 ), DNAG_NEIMA (P57028 ), DNAG_NEIMB (P57029 ), DNLJ1_STRGG (B1VUC2 ), DNLJ_FERNB (A7HJG6 ), DRD1_CARAU (P35406 ), EXOC1_DICDI (Q54NV1 ), FIXJ_AZOC5 (P26487 ), FLII_BUCBP (Q89AZ7 ), FMT_GEOSL (Q74GW4 ), FOLD2_ACIAD (Q6F8N7 ), FOLD_ACIBT (A3M846 ), FOLD_CALS4 (Q8RAD0 ), FOLD_MYCPE (Q8EV80 ), FOLD_PSYA2 (Q4FTX6 ), FOLD_PSYCK (Q1QD30 ), FOLD_RUTMC (A1AXA0 ), GLUQ_PROAC (Q6A7Y1 ), GLYA_MYCS2 (A0R2V7 ), GLYA_PROAC (Q6AAU3 ), GUAAB_PICTO (Q6L1Q1 ), GUAA_ASPOR (Q2UFN0 ), HEM1_RHORT (Q2RWE2 ), HIS5_ECOL6 (P60596 ), HIS5_ECOLI (P60595 ), HIS5_SHIBS (Q323I9 ), HIS5_SHIDS (Q32EF2 ), HIS5_SHIFL (Q83KJ5 ), HIS5_SHISS (Q3Z0G2 ), HLYE_ECO57 (Q9REB3 ), HLYE_ECOLI (P77335 ), ...
Mile and nautical mile are both units used to measure distances. The main difference between mile and nautical mile is that the mile is 1.609344 km while...
Damn the pain!. Yesterdays five-miler started out great. After the first warm-up mile, the second mile, without trying too hard, was 6:26.. My best opening mile since July 2007.. The opening mile is actually the second mile - the very first mile is simply a warm-up, in the 8-9 minute range.. Mile three is a recovery mile with slower jogging, three minutes of backwards jogging, some walking and stretching, etc. Mile four is designed to be the fastest mile of the five. I use this for conditioning and especially for learning to gauge pace. I mentally aim for a mile time, then study my 400, 800, and 1,200 split times to see how my pace is going.. I also try to run negative splits, meaning, the second half is faster than the first half. Last month, a did a mile time trial, and clocked 5:38, with 2:53 and 2:45 splits.. I had juice left at the end, because I was running about 90%. Going a full 100% felt risky, since I hadnt run that hard in nealy two years.. Yesterday, however, at the mile ...
TY - JOUR. T1 - Immunohistologic demonstration of coxiella burnetii in the valves of patients with Q fever endocarditis. AU - Brouqui, Philippe. AU - Dumler, J. Stephen. AU - Raoult, Didier. PY - 1994/11. Y1 - 1994/11. N2 - purpose: Cardiac valves that were resected from patients with Q fever endocarditis were examined by immunohistologic methods to correlate the presence of Coxiella burnetii in the valves with the histopathologic, serologic, microbiologic, and clinical findings. patients: Seventeen patients with serologic and microbiologic or clinical evidence of Q fever endocarditis who presented with cardiac failure secondary to valvular dysfunction and required valve replacement surgery were selected from the clinical records of the Unité des Rickettsies, Marseille, France. methods: Clinical data were collected by questionnaire. Serologic characterization was performed by indirect immunofluorescent antibody testing; shell vial cultivation of C burnetii was performed from resected valves and ...
BACKGROUND: Q fever is caused by the intracellular bacterium Coxiella burnetii. Initial infection can present as acute Q fever, while a minority of infected individuals develops chronic Q fever endocarditis or vascular infection months to years after initial infection. Serology is an important diagnostic tool for both acute and chronic Q fever. However, since immunosuppressive drugs may hamper the humoral immune response, diagnosis of Q fever might be blurred when these drugs are used. CASE PRESENTATION: A 71-year-old Caucasian male was diagnosed with symptomatic acute Q fever (based on positive C. burnetii PCR followed by seroconversion) while using anti-tumor necrosis factor-alpha (anti-TNFalpha) drugs for rheumatoid arthritis (RA). He was treated for two weeks with moxifloxacin. After 24 months of follow-up, the diagnosis of probable chronic Q fever was established based on increasing anti-C. burnetii phase I IgG antibody titres in a immunocompromised patient combined with clinical suspicion ...
The first case of Q fever in Taiwan was reported in 1993. The disease is considered to be emerging in Taiwan, but the route of transmission has remained unclear. The annual number of confirmed Q fever cases has been increasing up to more than 100 cases since 2005, comparing with less than 30 before 2003. The purpose of this study was to determine the seroprevalence and risk factors of Coxiella burnetii infection in veterinary-associated populations in southern Taiwan. A total of 228 serum samples of high risk individuals engaging in veterinary-related work or animal-farm work, were collected between March and June in 2007. The study individuals were interviewed by a structured questionnaire designed for Q fever investigation. Serum samples from different animal species were also obtained for Q fever analysis in the same study areas. Serological test was conducted by indirect immunofluorescence antibody assay (IFA). The result demonstrated the overall seroprevalence of Q fever was 26.3% in ...
Q fever bacteria (Coxiella burnetii, yellow), coloured transmission electron micrograph (TEM). Q fever is a rare livestock disease that can be spread to humans through inhalation of contaminated particles. It is considered the worlds most infectious disease, as just one bacterium is capable of causing infection. It causes flu-like symptoms including fever, headache and nausea. It can also lead to hepatitis, pneumonia or inflammation of the heart lining, all of which can be fatal. Magnification: x900 when printed 10 centimetres wide. - Stock Image C002/5597
Q fever, a rickettsial infection caused by Coxiella burnetii, has been recognized as a widely distributed zoonosis with the potential for causing both sporadic and epidemic disease. The resistance of Coxiella burnetii to heat, chemical agents, and desiccation allows the agent to survive for extended periods outside the host.. The infection is spread by the inhalation of infected material, mainly from sheep and goats. They shed the organism in feces, milk, nasal discharge, placental tissue, and amniotic fluid.. The clinical spectrum of disease ranges from unapparent to fatal. Respiratory manifestations usually predominate; endocarditis and hepatitis can be complications.. During the course of the infection, the outer membrane of the organism undergoes changes in its lipopolysaccharide structure, called phase variation. Differences in phase I and phase II antigen presentation can help determine if the infection is acute or chronic:. -In acute Q fever, the phase II antibody is usually higher than ...
To increase understanding of human bacterial and parasitic pathogens in bats, we investigated the prevalence of Babesia spp., Rickettsia spp., Anaplasma spp. and Coxiella burnetii in bats from China. Bats were captured from Mengyin County, Shandong Province of China using nets. DNA was extracted from the blood and spleen of bats for molecular detection of Babesia spp., Rickettsia spp., Anaplasma spp. and Coxiella burnetii with specific primers for each species. A total of 146 spleen samples and 107 blood samples of insectivorous bats, which belonged to 6 species within two families, were collected from Mengyin County, Shandong Province of China. We found that two Eptesicus serotinus (2/15, 13.3%) were positive for Babesia vesperuginis. We were unable to detect genomic sequences for Rickettsia spp., Anaplasma spp. and Coxiella burnetii. To our knowledge, our study showed for the first time the presence of Babesia vesperuginis in Eptesicus serotinus collected from China, suggesting that Babesia
For the detection and semi-quantitation of IgM antibodies to Coxiella burnetii, the etiologic agent of Q fever in human serum. Slide wells have individual spots of C. burnetii phase I and C. burnetii phase II in each well.. ...
Q fever is a disease of humans, caused by Coxiella burnetii, and a large range of animals can be infected. This paper presents a review of the epidemiology of Q fever in humans and farm animals between 1982 and 2010, using case studies from four European countries (Bulgaria, France, Germany and the Netherlands). The Netherlands had a large outbreak between 2007 and 2010, and the other countries a history of Q fever and Q fever research. Within all four countries, the serological prevalence of C. burnetii infection and reported incidence of Q fever varies broadly in both farm animals and humans. Proximity to farm animals and contact with infected animals or their birth products have been identified as the most important risk factors for human disease. Intrinsic farm factors, such as production systems and management, influence the number of outbreaks in an area. A number of disease control options have been used in these four countries, including measures to increase diagnostic accuracy and general
Recent reports have shown that some of the immunological aspects of Q fever, a rickettsiosis caused by Coxiella burnetii, could be related to self-antigen responses. The aim of this study was to determine the specificity of the autoantibody response of patients with acute and chronic Coxiella infections. Smooth muscle and cardiac muscle-specific autoantibodies were observed in significant percentages in acutely or chronically affected Q fever patients when compared to healthy volunteers. Moreover, the incidence of cardiac muscle-specific autoantibody was significantly higher among chronically ill patients compared to acutely ill patients. Moreover, a band of 50 kD of a HeLa extract was detected in most of the sera of individuals with chronic infections and previous sequence analysis suggests that this antigen presents a high degree of homology with the human actin elongation factor 1 alpha. Further research would be necessary to confirm if antibodies to human cytoskeletal proteins could be of clinical
Main Article. The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.. ...
Coxiella burnetii is the etiological agent of the worldwide distributed zoonosis Q fever. This review gives a comprehensive overview about the etiological agent and epidemiology of C. burnetii. Moreover, the role of arthropod vectors in transmission of C. burnetii is discussed. Detailed information is provided about current diagnostics and vaccination and importantly the most recent developments for improving the respective measures in animals. Immunoproteomic approaches are presented aimed to replace currently used whole cell antigen with well-defined and reproducible epitopes. Ruminants are considered as the main reservoir for human infections. The often subclinical infection in animals and suboptimal sensitivity of diagnostics hamper the identification of infected animals, while effectiveness of the current vaccine is questionable. New strategies based on advanced diagnostics and vaccines in animals will have the potential to prevent Q fever spreading and transmission to humans.
Q fever is a disease acquired from animals caused by Coxiella burnetii, a rickettsialike organism of low virulence but remarkable infectivity. A single organism can cause illness. In addition, despite the fact that C burnetii is unable to grow or replicate outside host cells, a sporelike form of the organism is extremely resistant to heat, pressure, and many disinfectants. This allows C burnetii to persist in the environment for long periods under harsh conditions. In contrast to this high degree of inherent resilience and transmissibility, the acute clinical disease associated with Q fever is usually a benign, although temporarily incapacitating, illness in humans ...
Q fever, or query fever, is caused by the bacterium Coxiella burnetti, an organism commonly found around farm animals. Q fever mainly affects pregnant female cats, resulting in weak newborns, stillbirths, and miscarriages. - Wag! (formerly Vetary)
In 1989, an outbreak of Q fever (C. burnetii infection) with 147 confirmed cases occurred in Solihull, West Midlands. Three patients developed cardiom
To assess Q fever in France, we analyzed data for 1985-2009 from the French National Reference Center. A total of 179,794 serum samples were analyzed; 3,723 patients (one third female patients) had acute Q fever. Yearly distribution of acute Q fever showed a continuous increase. Periodic variations were observed in monthly distribution during January 2000-December 2009; cases peaked during April-September. Q fever was diagnosed more often in patients in southeastern France, where our laboratory is situated, than in other areas. Reevaluation of the current positive predictive value of serologic analysis for endocarditis was performed. We propose a change in the phase I (virulent bacteria) immunoglobulin G cutoff titer to &gt;1,600. Annual incidences of acute Q fever and endocarditis were 2.5/100,000 persons and 0.1/100,000 persons, respectively. Cases and outbreaks of Q fever have increased in France.
Context Diagnosis of infective endocarditis (IE), particularly in blood culture-negative patients, has been a problem and requires further investigations. Aim This study was designed to evaluate the added value of a commercial multiplex PCR performed on blood in the early diagnosis of IE and compare its performance with conventional blood culture and serological testing, at a tertiary care center in Egypt. Settings and design Thirty-seven cases of diagnosed definite endocarditis were enrolled in the study. Patients and methods For each patient, blood culture was prepared and serum sample was obtained for serology testing for Brucella spp., Bartonella spp., Coxiella burnetii antibodies, and Aspergillus galactomannan antigen. Patients were selected for commercial Seegene multiplex PCR (sepsis screening) when their blood culture remained negative after 24 h incubation. Statistical analysis Sensitivity, specificity, positive predictive value, negative predictive value, and κ-test were used in ...
SUMMARYAs Q fever is associated with an inflammatory syndrome, we determined circulating levels of inflammatory cytokines, cytokine antagonists, and activation markers of leucocytes in patients with acute Q fever and Q fever endocarditis. Tumour necrosis factor (TNF) and IL-6, but not IL-1β, were ma
Q fever is a widespread zoonotic infection caused by the pathogen,Coxiella burnetii. The designation Q fever (from Query) was made in 1935 following an outbreak of a febrile illness in slaughterhouse workers in Queensland, Australia. The disease is r
Q Fever is a rickettsial disease caused by Coxiella burnetii. Humans can get the disease from infected animal tissues or possibly through insect and tick bites.
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adsbygoogle = window.adsbygoogle ,, []).push({}); * Due to an intracellular bacteria (macrophages) Coxiella burnetii. * This is a ubiquitous zoonosis; human contamination ...
Un instituto de investigación eslovaco ha desarrollado nuevos antígenos corpusculares de Coxiella burnetii y algunas especies de chlamidya y rickettsia. Estos antígenos se han diseñado para medir de forma precisa anticuerpos contra estas bacterias en muestras de suero, plasma y leche de animales y humanos. También pone a disposición una vacuna corpuscular eficiente para la profilaxis contra coxielosis en ganado. El instituto está interesado en establecer acuerdos de licencia o joint venture ...
Video articles in JoVE about high content screening include Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening, A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening, High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC), Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants, High Content Screening in Neurodegenerative Diseases, High Content Screeningはゆゆェソトリゲェトは祥絍紬胧は毐怣ェトソェ - ADVERTISEMENT, A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos, A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules, A High-content In Vitro Pancreatic Islet β-cell Replication Discovery Platform, 3D Microtissues for Injectable Regenerative Therapy and
3SLH: 1.70 Angstrom resolution structure of 3-phosphoshikimate 1-carboxyvinyltransferase(AroA) from Coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
Most patients with Q fever endocarditis require 18 months of antibiotic therapy to protect native heart valves and 24 months for prosthetic valves, data from a French registry suggest.
Coxiella burnetti can induce abortion in domestic mammals (cat, dog, rabbit) and ruminants, and these animals represent their main reservoir. The danger posed by Coxiella is that they can be excreted by animals exhibiting no apparent clinical signs of the disease, whether following natural infection or because the animals have been vaccinated with an ineffective vaccine. This can then be tranported to humans via inhalation or tick bite ...
As with other Sciences departments, the Physics Department has a student organization on campus. The CBU chapter of the Society of Physics Students (SPS) is open to all students with an interest in physics. Dr. Ted Clarke serves as the faculty moderator. The chapter has been active this year holding a Science Trivia Night with CBU and Rhodes SPS in fall, helping out with the Science Olympiad where they served as supervisors for 3 events and assisted with 5 other events, and assisting with the Science Fair.. Faculty members in the department are committed to teaching physics and continually hone their craft. Dr. Clarke was elected TAAPT president for the 2014-15 year. CBU will host the conference in the coming spring (2015).. The department will offer honors sections of Physics I and Physics II beginning in the fall 2014 semester. These sections are open to students that participate in the Honors Program (http://www.cbu.edu/academicsupport/honors/) at CBU. These two courses, PHYS 150 and 251, ...
Get info about CBU tissue printing, registering in a technical training program, and taking free tests online. Seek out nationally-accredited engineering programs for the best training. Given a brief internship or prior work-experience, you can expect a great return on your education.
How Q fever bacteria spread from animals to humans, symptoms, treatment for Q fever, who is at risk, and how Q fever can be prevented.
Cerebral palsy (CP) is a group of neurodevelopmental conditions with abnormal movement and posture resulted from a non-progressive cerebral disturbance. It is the most common cause of motor disability in childhood. Most therapies are palliative rather than restorative. Umbilical cord blood (UCB) may be used as restorative approach for children with CP.. Many experimental animal studies have revealed that UCB is beneficial to improve and repair neurological injuries, this effect achieved due to immune regulation and angiogenesis as well as the neuroprotective effect.. Based on animal studies and some clinical trials, UCB is suggested as a potential therapy for children with CP.. This study is prospective, non randomized (open label) with control group. СLINICAL PURPOSES Estimation of the efficiency of the method of transfusion of umbilical blood hematopoietic cells to patients with diagnosed infantile cerebral palsy depending on the degrees of compatibility of donor and recipient.. RESEARCH ...
Does anyone know where I may find some ino about q fever, or query fever, on trhe internet. Please contact ASNT at airmail.net. Thanks ...
N.C. Communicable Disease Branch page for Q fever. Includes a definition of the illness, prevention info, and links to relevant CDC resources.
Where did February go?? January wasnt too bad but February has been intense. Where I was at the start of February. I managed to make plasmid #8, and I was in the process of putting it into Coxiella and checking to make sure the yellow-red protein was being made. I was also trying to make…
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October 2018 P hD t ion s - MRC Cognition and Brain Sciences Unit Reading and Download User Manuals and Owners Guides of application of cbu 2018 intake
Volkswagen has launched its super-successful New Beetle in the Indian market today. The car has been introduced with a price tag of Rs 20.45 lakh, ex-showroom Delhi. The car will be available as a CBU through Volkswagen dealerships. The New ... Read More ». ...
An average person can walk one mile on a flat, well-groomed surface in 17 to 20 minutes. A fit person walking briskly can walk a mile in 13 minutes. An estimated average walking speed for calculating...
Race Description Out and Back One Mile Run on the Blue Ash Taxi Runway during Summer at Summit Park in Blue Ash. The Mile Run where we would like to see...
The Inn on the Mile: Rooms were decent sized and extremely comfortable - See 1,220 traveller reviews, 382 candid photos, and great deals for The Inn on the Mile at TripAdvisor.
adaa....tgh ready nk g keje la ni..tapi....ukail....bangunlaa...ummi nk g keje ni..nanti guru besar marah ummi lak..kang ummi simbah air kat muke kang ...
Dennis Stevenson was delivering mail one freezing-cold day a couple of years ago when an elderly woman stopped him at her door. Her heat was out, she said, and she couldn`t find anyone to fix
Last year I got in 787 miles, still short of 1000, and short of my total for the last two years. But thats why we have another year right? Im not really sure where I got derailed but anyway, I did run three half-marathons, seven 5ks, five 10ks, four 15ks, one 8k, and one 4 mile race. We also raced in 5 states. Here is my collection of hardware for the year ...
Coxiella burnetii)" [Is A29, B12 vasculitis caused by the Q fever agent? (Coxiella burnetii)]. Journal Français d'Ophtalmologie ... Coxiella burnetii - named for Cox and Burnet - is no longer regarded as closely related to the Rickettsiae, but as similar to ... Q fever or query fever is a disease caused by infection with Coxiella burnetii, a bacterium that affects humans and other ... Camuset, P; Remmy, D (2008). "Q Fever (Coxiella burnetii) Eradication in a Dairy Herd by Using Vaccination with a Phase 1 ...
Coxiella burnetii, Bartonella spp., Borrelia burgdorferi, Venezuelan equine encephalitis virus, Eastern equine encephalitis ...
Coxiella burnetii Causes Q fever. Francisella tularensis Causes tularemia. Legionella pneumophila Causes a severe form of ...
Coxiella burnetii is also a coccobacillus. Bacteria from the genus Brucella are medically important coccobacilli that cause ...
Coxiella (i.e. Coxiella burnetii). *Dendrosporobacter. *Desulfotomaculum. *Desulfosporomusa. *Desulfosporosinus. * ...
It is a potential vector for Coxiella burnetii. "Species Details : Amblyomma gervaisi Lucas, 1847". Catalogue of Life. ...
Coxiella burnetii is a species in this order. Another is Rickettsiella melolonthae. Legionellales "List of genera included in ...
Coxiella burnetii (Agent OU), causative agent of Q fever; and Bacillus globigii (Agent BG). During Project SHAD, Bacillus ...
Also, H. leporispalustris transmits Coxiella burnetii and Francisella tularensis. Strains of Anaplasma bovis have also been ...
... s may serve as a primary reservoir for Coxiella burnetii. Infection is transmitted among them by ticks. These are then ...
However, in 2009 scientists reported a technique allowing the Q-fever pathogen Coxiella burnetii to grow in an axenic culture ... "Host cell-free growth of the Q fever bacterium Coxiella burnetii". Proceedings of the National Academy of Sciences USA. 106 (11 ... Rickettsia Coxiella Certain species of Mycobacterium such as Mycobacterium leprae Certain protozoa, including: Apicomplexans ( ...
Wachter, R. F.; Briggs, G. P.; Pedersen, C. E. (November 1975). "Precipitation of phase I antigen of Coxiella burnetii by ...
Wachter, R. F.; Briggs, G. P.; Pedersen, C. E. (1975). "Precipitation of phase I antigen of Coxiella burnetii by sodium sulfite ...
2003). "Complete genome sequence of the Q-fever pathogen Coxiella burnetii". Proc. Natl. Acad. Sci. U.S.A. 100 (9): 5455-60. ...
"Biochemical stratagem for obligate parasitism of eukaryotic cells by Coxiella burnetii". Proceedings of the National Academy of ... They either reproduce inside of the phagolysosome (e.g. Coxiella spp.) or escape into the cytoplasm before the phagosome fuses ...
2009). "Host cell-free growth of the Q fever bacterium Coxiella burnetii". PNAS. 106 (11): 4430-4434. Bibcode:2009PNAS.. ... Coxiella burnetii. Asepsis Gnotobiotic animal Germ-free animal Sterilization (microbiology) Thain, M.; Hickman, M. (1994). ...
Complete inactivation of Coxiella burnetii (which was thought at the time to cause Q fever by oral ingestion of infected milk) ... Enright, J.B.; Sadler, W.W.; Thomas, R.C. (1957). "Thermal inactivation of Coxiella burnetii and its relation to pasteurization ... Cerf, O.; Condron, R. (2006). "Coxiella burnetii and milk pasteurization: an early application of the precautionary principle ... Practice for Milk notes that milk pasteurization is designed to achieve at least a 5 log10 reduction of Coxiella burnetii. The ...
Coxiella burnetii, the causative agent of Q fever, thrives and replicates in the acidic phagolysosomes of its host cell. The ... Maurin, M.; Benoliel, A. M.; Bongrand, P.; Raoult, D. (1992-12-01). "Phagolysosomes of Coxiella burnetii-infected cell lines ... Howe, Dale; Mallavia, Louis P. (2016-11-19). "Coxiella burnetii Exhibits Morphological Change and Delays Phagolysosomal Fusion ... acidity of the phagolysosome is essential for C.burnetii to transport glucose, glutamate, and proline, as well as for its ...
... is also commonly mentioned as a potential vector of Q fever (Coxiella burnetii). The ornate kangaroo tick ( ...
"Coxiella burnetii". Proc Natl Acad Sci U S A. 78 (5): 3240-3244. doi:10.1073/pnas.78.5.3240. PMC 319537. PMID 6942430.. .mw- ... They either reproduce inside of the phagolysosome (e.g. Coxiella spp.)[1] or escape into the cytoplasm before the phagosome ...
1995). "Q fever (Coxiella burnetii) reservoir in wild brown rat (Rattus norvegicus) populations in the UK". Parasitology. 110: ... In the United Kingdom, brown rats are an important reservoir for Coxiella burnetii, the bacterium that causes Q fever, with ...
Atypical bacteria causing pneumonia are Coxiella burnetii, Chlamydophila pneumoniae (J16.0), Mycoplasma pneumoniae (J15.7), and ... erythromycin Coxiella burnetti - doxycycline Legionella pneumophila - erythromycin, with rifampicin sometimes added. People who ...
"Specificity of Legionella pneumophila and Coxiella burnetii vacuoles and versatility of Legionella pneumophila revealed by ... Legionella has a correlation with another genus called Coxiella. Both cause lung infection that can eventually lead to ...
Duron O (September 2015). "The IS1111 insertion sequence used for detection of Coxiella burnetii is widespread in Coxiella-like ... Since Coxiella and Francisella endosymbionts are closely related to pathogens, there is a substantial risk of misidentification ... Although Coxiella and Francisella endosymbionts are distantly related bacteria, they have converged towards an analogous B ... December 2017). "A Coxiella mutualist symbiont is essential to the development of Rhipicephalus microplus". Scientific Reports ...
For example, Q fever is caused by Coxiella burnetii and Legionella pneumophila causes Legionnaires' disease and Pontiac fever. ... They comprise two families, typified by Legionella and Coxiella, both of which include notable pathogens. ...
... the first vaccine against Coxiella burnetii, the bacteria that causes Q fever. He observed that Q fever led to a post-viral ...
Chlamydophila pecorum and Coxiella burnetii from aborted ruminant's clinical samples using multiplex PCR". BMC Microbiology. 9 ...
"Chronic fatigue following infection by Coxiella burnetii (Q fever): ten-year follow-up of the 1989 UK outbreak cohort". QJM. 95 ...
Under Housewright's direction, the scientists at Fort Detrick developed a cocktail of Coxiella burnetii, the causative agent of ...
Coxiella burnetii, and rickettsia species. Excessive alcohol consumption is a significant cause of hepatitis and is the most ...
1995). "Q fever (Coxiella burnetii) reservoir in wild brown rat (Rattus norvegicus) populations in the UK". Parasitology. 110: ... In the United Kingdom, brown rats are an important reservoir for Coxiella burnetii, the bacterium that causes Q fever, with ...
Coxiella burnetii[28]. *Ehrlichia[29]. *Leptospira. *Listeria[30]. *Mycobacterium tuberculosis[31]. *Nocardia[32] ...
Coxiella burnetii livestock and other domestic animals such as dogs and cats inhalation of spores, contact with bodily fluid or ...
Coxiella burnetii *Q fever. Thiotrichales. *Francisella tularensis *Tularemia. Vibrionaceae. *Vibrio cholerae *Cholera ...
However, in 2009 scientists reported a technique allowing the Q-fever pathogen Coxiella burnetii to grow in an axenic culture ... "Host cell-free growth of the Q fever bacterium Coxiella burnetii". Proceedings of the National Academy of Sciences USA. 106 (11 ...
Coxiella. *Coxiella burnetii. E. *Eksperimen evolusi jangka panjang E. coli. *Escherichia coli ...
Coxiella burnetii *Q fever. Thiotrichales. *Francisella tularensis *Tularemia. Vibrionaceae. *Vibrio cholerae *Cholera ...
Coxiella burnetii. Q fever. Number of bacteria needed to infect a person is one - the lowest known to man[6]p.67. Can live on ...
Legionella pneumophila/Legionella longbeachae (Legionellosis) · Coxiella burnetii (ക്യു പനി). Thiotrichales. Francisella ...
Coxiella. *Coxiella burnetii. D. *Desulfotomaculum. E. *Enterobacter sakazakii. *Enterococcus. *Bacillus thuringiensis. * ...
Coxiella burnetii. Q fever. Q fever vaccine. Q-Vax Haemophilus influenzae type B (Hib). Epiglottitis, meningitis, pneumonia. ...
Coxiella burnetii *Q fever. Thiotrichales. *Francisella tularensis *Tularemia. Vibrionaceae. *Vibrio cholerae *Cholera ...
Coxiella burnetii, Venezuelan equine encephalitis virus, Staphylococcal enterotoxin B). The use of chemicals to induce altered ...
Coxiella burnetii, agen penyebab demam Q, tumbuh subur dan bereplikasi dalam fagolisosom berkat kondisi asam dari sel inangnya. ... "Phagolysosomes of Coxiella burnetii-infected cell lines maintain an acidic pH during persistent infection". Infection and ... "Coxiella burnetii Exhibits Morphological Change and Delays Phagolysosomal Fusion after Internalization by J774A.1 Cells". ... 6] Keasaman fagolisosom sangat penting bagi C. burnetii untuk mengangkut glukosa, glutamat, dan prolin, serta untuk sintesis ...
Coxiella burnetii Rabies Rabies virus Relapsing fever Borrelia hermsii, Borrelia recurrentis, and other Borrelia species ...
Legionella pneumophila/Legionella longbeachae (Legionellosis) · Coxiella burnetii (Q fever). Thiotrichales. Francisella ...
Coxiella burnetii *Q fever. Thiotrichales. *Francisella tularensis *Tularemia. Vibrionaceae. *Vibrio cholerae *Cholera ...
Enright, J.B.; Sadler, W.W.; Thomas, R.C. (1957). "Thermal inactivation of Coxiella burnetii and its relation to pasteurization ... Complete inactivation of Coxiella burnetii (which was thought at the time to cause Q fever by oral ingestion of infected milk)[ ... "Coxiella burnetii and milk pasteurization: an early application of the precautionary principle?". Epidemiology & Infection. 134 ... Practice for Milk notes that milk pasteurization is designed to achieve at least a 5 log10 reduction of Coxiella burnetii.[47] ...
Chlamydia pneumoniae, Chlamydia psittaci, Legionella pneumophila, Coxiella burnetii, respiratorni sincicijski virus, virusi ... Izpostavljenost pticam je v zvezi s Chlamydia psittaci, izpostavljenost domačim živalim s Coxiella burnetti; vdihavanje ...
Coxiella burnetii *Q fever. Thiotrichales. *Francisella tularensis *Tularemia. Vibrionaceae. *Vibrio cholerae *Cholera ...
Coxiella burnetii. *Ebola. *Equine encephalitis (Eastern. *Western. *Venezuelan). *Foodborne illness. *Fungi. *Glanders ...
Coxiella burnetii (Q-fever) Venezuelan equine encephalitis virus (VEE) Botulinum toxin (botulism) Staphylococcal enterotoxin B ...
Hackstadt T and Williams J C "Biochemical stratagem for obligate parasitism of eukaryotic cells by Coxiella burnetii." Proc ... é o caso da bacteria Coxiella burnetii.[1] Outras pasan ao citoplasma antes de que o fagosoma se fusione co lisosoma, como ...
The cause was found to be Coxiella burnetii, with their findings published in 1948 in the American Journal of Public Health. ... Huebner found that the C. burnetii bacteria could survive temperatures of up to 60 °C (140 °F) in sealed containers for as long ...
... the exposure of volunteers to aerosols containing a highly pathogenic strain of Coxiella burnetii, the causal agent of Q fever ...
By the late 1970s, in addition to the work on Coxiella burnetii and other rickettsiae, research priorities had expanded to ... the exposure of volunteers to aerosols containing a highly pathogenic strain of Coxiella burnetii, the causal agent of Q fever ...
Coxiella burnetii is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. The genus Coxiella is ... C. burnetii, the causative agent of Q fever Shaw EI, Voth DE (January 2019). "Coxiella burnetii: A Pathogenic Intracellular ... Coxiella burnetii small RNAs (CbSRs 1, 11, 12, and 14) are encoded within intergenic region (IGR). CbSRs 2, 3, 4 and 9 are ... Sankaran N (2000). "Coxiella burnetii". Microbes and people : an A-Z of microorganisms in our lives. Phoenix, Arizona: The Oryx ...
Coxiella burnetii is a bacterial intracellular parasite of eucaryotic cells that replicates within a membrane-bound compartment ... The Coxiella burnetii parasitophorous vacuole.. Ghigo E1, Colombo MI, Heinzen RA. ... C. burnetii resists the degradative functions of the vacuole while at the same time exploiting the acidic pH for metabolic ... burnetii lipopolysaccharide phase variants in human mononuclear phagocytes. ...
Coxiella burnetii. Q Fever. The pathogens occur all over the world and can result in a Q fever infection in humans. The ... Coxiella (C.) burnetii is a pleomorphic (rod-like, coccoid lanceolate) gram-variable bacterium from the family of ... Coxiella brunetii has a broad host range but is detected primarily in sheep, goats, cattle and wild ruminants. In natural herds ... C. burnetii is characterised by a high resistance to chemical and physical influences. The duration of survival is particularly ...
... caused by infection of the bacteria Coxiella burnetii) is an animal-to-human infection. The illness brings severe flu-like ... Preventing Coxiella burnetii Infection * {"smallUrl":"https:\/\/www.wikihow.com\/images\/thumb\/1\/10\/Prevent-Q-Fever-% ... Know if you are at risk. Coxiella burnetii is excreted in the milk, urine, and feces of infected animals. Cows, sheep, and ... Based on where your facility is located, you may or may not be able to legally use the Coxiella burnetii vaccine.[10] X ...
Q fever, caused by the pathogen Coxiella burnetii, is an acute disease that can progress to become a serious chronic illness. ...
Fusogenicity of the Coxiella burnetii parasitophorous vacuole.. Howe D1, Melnicákova J, Barák I, Heinzen RA. ... burnetii protein synthesis and replication is required for maintenance of the fusogenic character of the Coxiella ... burnetii protein synthesis, but not replication, is required for homotypic fusion and maturation of nascent C. burnetii PV. We ... J774A.1 murine macrophage-like cells with mature C. burnetii PV were incubated with latex beads and the trafficking of beads to ...
Characterization of Coxiella burnetii outbreak strains. Status: Completed Start project:. Sep 1, 2011. End project:. Sep 1, ... Characterization of Coxiella burnetii outbreak strains Q fever is a worldwide zoonotic infectious disease caused by the ... burnetii outbreak strains. This thesis research focused on the characterization of C. burnetii outbreak strains isolated from ... bacterium Coxiella burnetii. During 2007-2010, the largest Dutch Q fever outbreak was reported, reporting more than 4000 human ...
To estimate the prevalence of Coxiella burnetii infection and identify associated risk factors, we conducted a study covering ... To estimate the prevalence of Coxiella burnetii infection and identify associated risk factors, we conducted a study covering ... We conclude that C. burnetii infection is prevalent among sheep producers in North Dakota. As the result, Q fever became a ... The number of sheep raised was a good predictor of C. burnetii infection. Lambing outdoors and frequent physical contacts with ...
... Start module. <;url> onclick=return prtflo_click(); target=_blank class=add_to_ ... Coxiella burnetii infections (Q fever) Rated -1/5 based on 0 customer reviews ... When to suspect Coxiella burnetii infection (Q fever), including both acute and chronic infections ... How to diagnose Coxiella burnetii infections *How to investigate for Coxiella burnetii infections ...
Coxiella Burnetii (Q Fever) Antibodies - Serum (Surplus) (ssqfev_c) Data File: ssqfev_c.xpt First Published: February 2008. ... Q fever is a zoonotic disease with acute and chronic stages caused by the rickettsia-like organism Coxiella burnetii. The ... The final results of C. burnetii infection was determined if Phase I or Phase II IgG IFA titer was , = 1:16. IFA Final ... and adapted to C. burnetii (purified phases I and II, strain Nine Mile; Rocky Mountain Laboratories, Hamilton, Mont.) as ...
Coxiella burnetii. Emerging Infectious Diseases. 2008;14(10):1566. doi:10.3201/eid1410.e11410.. ... 2008). Coxiella burnetii. Emerging Infectious Diseases, 14(10), 1566. https://dx.doi.org/10.3201/eid1410.e11410.. ... C. burnetii belongs in the family Coxiellaceae, which consists of gram-negative rods without flagella or a capsule. The ... Coxiella burnetii. Emerg Infect Dis. 2008;14(10):1566. https://dx.doi.org/10.3201/eid1410.e11410. ...
Pentose phosphate pathway - Coxiella burnetii CbuK_Q154 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Homologous recombination - Coxiella burnetii Dugway 5J108-111 [ Pathway menu , Organism menu , Pathway entry , Download KGML , ...
Coxiella burnetii (strain RSA 331 / Henzerling II). Coxiella burnetii (strain RSA 493 / Nine Mile phase I). Coxiella burnetii. ... Coxiella burnetii (strain RSA 331 / Henzerling II). Coxiella burnetii (strain RSA 493 / Nine Mile phase I). Coxiella burnetii. ... sp,A9KF61,TYSY_COXBN Thymidylate synthase OS=Coxiella burnetii (strain Dugway 5J108-111) GN=thyA PE=3 SV=1 ... cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › CoxiellaCoxiella ...
Coxiella burnetii Intratracheal Aerosol Infection Model in Mice, Guinea Pigs, and Nonhuman Primates Coxiella burnetii, the ... Coxiella burnetii Blocks Intracellular Interleukin-17 Signaling in Macrophages Coxiella burnetii is an obligate intracellular ... Coxiella burnetii Requires Host Eukaryotic Initiation Factor 2α Activity for Efficient Intracellular Replication Coxiella ... Viable Coxiella burnetii Induces Differential Cytokine Responses in Chronic Q Fever Patients Compared to Heat-Killed Coxiella ...
Coxiella burnetii. Mutation(s): 0 Gene Names: CBU_0775. Find proteins for Q83DG1 (Coxiella burnetii (strain RSA 493 / Nine Mile ... Coxiella burnetii is a highly infectious bacterium and potential agent of bioterrorism. However, it has not been studied as ... Coxiella burnetii is a highly infectious bacterium and potential agent of bioterrorism. However, it has not been studied as ... Structural genomics for drug design against the pathogen Coxiella burnetii.. Franklin, M.C., Cheung, J., Rudolph, M.J., ...
Coxiella burnetii:. C burnetii is a zoonotic bacterial infection associated primarily with parturient ruminants although ... Coxiella burnetii has been detected on four farms in 2018 in four Colorado counties. ... C burnetii is a reportable disease in Colorado (to the State Veterinarians Office and Colorado Department of Public Health and ... C burnetii is common in livestock and animal testing does have limitations, as shedding can be intermittent. ELISA and PCR ...
... burnetii are different from that of Coxiella-like bacteria found in ticks. Horizontally acquired genes that allow C. burnetii ... burnetii are different from that of Coxiella-like bacteria found in ticks. Horizontally acquired genes that allow C. burnetii ... very little is known about genes that facilitate Coxiellas intracellular growth. Recent studies indicate that C. burnetii ... very little is known about genes that facilitate Coxiellas intracellular growth. Recent studies indicate that C. burnetii ...
High numbers of C. burnetii were excreted during abortion, but also during parturition of liveborn kids. C. burnetii was not ... C. burnetii has a strong tropism for the trophoblasts of the placenta and is not excreted before parturition; pathogen ... The amount of C. burnetii DNA in the organs of goats and kids increased towards parturition. After parturition it decreased to ... Besides abortions, normal deliveries in C. burnetii-infected goats should be considered as a major zoonotic risk for Q fever in ...
Coxiella burnetii. Coxiella burnetii (strain CbuK_Q154) (Coxiella burnetii (strain Q154)). Coxiella burnetii (strain RSA 331 / ... Coxiella burnetii (strain Dugway 5J108-111). Coxiella burnetii (strain CbuK_Q154) (Coxiella burnetii (strain Q154)). Coxiella ... Coxiella burnetii (strain RSA 493 / Nine Mile phase I). Coxiella burnetii. 465. UniRef90_A9KBJ3. Cluster: Glutamate--tRNA ... Coxiella burnetii (strain CbuG_Q212) (Coxiella burnetii (strain Q212)). ,p>This subsection of the ,a href="http://www.uniprot. ...
Identification of rickettsiae isolated in Japan as Coxiella burnetii by 16S rRNA sequencing. Int J Syst Bacteriol. 1997;47:883- ... Coxiella burnetii. isolated from an Amblyomma parvum. tick. Note large vacuoles enclosing a seething mass of microorganisms. ... Coxiella burnetii in Ticks, Argentina. Emerging Infectious Diseases. 2013;19(2):344-346. doi:10.3201/eid1902.120362.. ... Pacheco, R. C., Echaide, I. E., Alves, R. N., Beletti, M. E., Nava, S., & Labruna, M. B. (2013). Coxiella burnetii in Ticks, ...
Coxiella burnetii RSA 331. Mutation(s): 0 Gene Names: clpP, COXBURSA331_A1213. EC: 3.4.21.92. ... Structure of the ClpP subunit of the ATP-dependent Clp Protease from Coxiella burnetii. Anderson, S.M., Wawrzak, Z., Gordon, E. ... Find proteins for Q83DJ2 (Coxiella burnetii (strain RSA 493 / Nine Mile phase I)) ... Structure of the ClpP subunit of the ATP-dependent Clp Protease from Coxiella burnetii. *DOI: 10.2210/pdb3Q7H/pdb ...
... and treatment information for Coxiella burnetii (Q fever) with alternative diagnoses, full-text book chapters, misdiagnosis, ... Coxiella burnetii. Description of Coxiella burnetii. Coxiella burnetii (medical condition): A disease caused by Coxiella ... Coxiella burnetii: Related Diseases. Coxiella burnetii: Coxiella burnetii is listed as a type of (or associated with) the ... Coxiella burnetii) Coxiella burnetii: Article Excerpts about Q fever Q fever is a zoonotic disease caused by Coxiella burnetii, ...
Evaluation of infected tissue, blood, or serum using PCR may be a useful tool for diagnosing some cases of Coxiella burnetii ... Mayo Medical Laboratories has developed a real-time PCR test that rapidly detects Coxiella burnetii DNA in clinical specimens ... by targeting a sequence of the shikimate dehydrogenase gene (aroE) unique to Coxiella burnetii. ... Coxiella burnetii, the causative agent of Q fever, is a small obligately intracellular bacterium, which is associated with ...
Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants in DOAJ. DOAJ is an online directory ... Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction ... Conversely, seronegative ruminants can shed C. burnetii. Rapid disintegration of C. burnetii DNA during the storage period is ... cattle were positive for Coxiella burnetii antibodies by enzyme linked immunosorbent assay kit (IDEXX, Liebefeld, Switzerland ...
Alfonso Carbonero, Lucía T. Guzmán, Karen Montaño, Alicia Torralbo, Antonio Arenas-Montes, Luis R. Saa, Coxiella burnetii ... The purpose of this study was to determine the seroprevalence and risk factors of Coxiella burnetii infection in veterinary- ... Mohammad Khalili, Morteza Mosavi, Hamzeh Ghobadian Diali, Hossein Norouzian Mirza, Serologic survey for Coxiella burnetii phase ... Identification of Risk Factors of Coxiella burnetii (Q fever) Infection in Veterinary-Associated Populations in Southern Taiwan ...
Mast Cell Cytonemes as a Defense Mechanism against Coxiella burnetii. Soraya Mezouar, Joana Vitte, Laurent Gorvel, Amira Ben ... Mast Cell Cytonemes as a Defense Mechanism against Coxiella burnetii. Soraya Mezouar, Joana Vitte, Laurent Gorvel, Amira Ben ... Mast Cell Cytonemes as a Defense Mechanism against Coxiella burnetii. Soraya Mezouar, Joana Vitte, Laurent Gorvel, Amira Ben ... Mast Cell Cytonemes as a Defense Mechanism against Coxiella burnetii Message Subject (Your Name) has forwarded a page to you ...
... supported excellent growth of Coxiella burnetii. Titration of the viable rickettsial content of samples taken during the growth ... Growth of Coxiella Burnetii in Agar Tissue Culture * Author(s): Edgar G. Pickens and Jakob A. Gaon ... supported excellent growth of Coxiella burnetii. Titration of the viable rickettsial content of samples taken during the growth ...
The widespread occurrence, maintenance and conservation of the IVS in C. burnetii imply that it plays an adaptive role or has a ... In spite of a high degree of IVS conservation among different strains of C. burnetii, the region immediately upstream of the ... In spite of a high degree of IVS conservation among different strains of C. burnetii, the region immediately upstream of the ... The widespread occurrence, maintenance and conservation of the IVS in C. burnetii imply that it plays an adaptive r... ...
Analysis of seroprevalence against Coxiella burnetii in a sample of farm workers in Western Sicily. MG Verso, G Vesco, S ... Prevalence of Coxiella burnetii antibodies in wild ruminants in Kavecany zoo, Kosice, eastern Slovakia.. Erik Dorko, Kvetoslava ... Serological prevalence of Coxiella burnetii in dairy goats and ewes diagnosed with adverse pregnancy outcomes in Greece. George ... Prevalence of Coxiella burnetii infection in foresters in the south-eastern Poland and comparison of diagnostic methods. Monika ...
  • Coxiella burnetii is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. (wikipedia.org)
  • C. burnetii, the causative agent of Q fever Shaw EI, Voth DE (January 2019). (wikipedia.org)
  • Q fever (caused by infection of the bacteria Coxiella burnetii ) is an animal-to-human infection. (wikihow.com)
  • Q fever, caused by the pathogen Coxiella burnetii , is an acute disease that can progress to become a serious chronic illness. (ingentaconnect.com)
  • Q fever is a worldwide zoonotic infectious disease caused by the bacterium Coxiella burnetii . (wur.nl)
  • In order to minimize such complications in the future, it was considered crucial to have a thorough understanding of C. burnetii virulence and to develop effective Q fever vaccines. (wur.nl)
  • Q fever is a zoonotic disease with acute and chronic stages caused by the rickettsia-like organism Coxiella burnetii. (cdc.gov)
  • Coxiella burnetii is the causative agent of human Q fever, eliciting symptoms that range from acute fever and fatigue to chronic fatal endocarditis. (asm.org)
  • The zoonotic bacterial pathogen Coxiella burnetii is the causative agent of Q fever, a febrile illness which can cause a serious chronic infection. (asm.org)
  • Coxiella burnetii , the etiological agent of Q fever, is a Gram-negative bacterium transmitted to humans by inhalation of contaminated aerosols. (asm.org)
  • Coxiella burnetii is an obligate intracellular Gram-negative bacterium which causes human Q fever. (asm.org)
  • Coxiella burnetii is an obligate intracellular bacterium and the etiological agent of Q fever. (asm.org)
  • Coxiella burnetii , the etiologic agent of acute Q fever and chronic endocarditis, has a unique biphasic life cycle, which includes a metabolically active intracellular form that occupies a large lysosome-derived acidic vacuole. (frontiersin.org)
  • Coxiella burnetii is the etiological agent of acute Q fever and a chronic disease commonly manifested as endocarditis ( Maurin and Raoult, 1999 ). (frontiersin.org)
  • The Gammaproteobacterium Coxiella burnetii is the causative agent of acute Q fever and chronic endocarditis in humans worldwide. (cdc.gov)
  • Coxiella burnetii (medical condition): A disease caused by Coxiella burnetti which causes fever. (rightdiagnosis.com)
  • Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed globally. (rightdiagnosis.com)
  • Coxiella burnetii , the causative agent of Q fever, is a small obligately intracellular bacterium, which is associated with animals. (mayomedicallaboratories.com)
  • 4. Fenollar F, Fournier PE, Raoult D: Molecular detection of Coxiella burnetii in the sera of patients with Q fever endocarditis or vascular infection. (mayomedicallaboratories.com)
  • Finally, this study documented that people with prior knowledge of Q fever were less likely to be seropositive for C. burnetii . (wiley.com)
  • Here, we describe a nonpreviously reported antibacterial mechanism used by MCs against Coxiella burnetii , the agent of Q fever. (asm.org)
  • The intervening sequence (IVS) of Coxiella burnetii , the agent of Q fever, is a 428-nt selfish genetic element located in helix 45 of the precursor 23S rRNA. (frontiersin.org)
  • INTRODUCTION: Coxiella burnetii, the causative agent of Q fever, may cause endocarditis and vascular infections that result in severe morbidity and mortality. (biomedsearch.com)
  • Coxiella burnetii , the causative organism of Q fever, is increasingly reported to be associated with infections of abdominal aortic aneurysms and vascular grafts. (biomedsearch.com)
  • Coxiella burnetii is a bacterium that causes Q fever, a zoonosis that affects large numbers of both humans and animals. (rivm.nl)
  • In 2009, field studies were started to investigate if C. burnetii DNA can be detected in aerosols on and in the near vicinity of Q fever affected farms. (rivm.nl)
  • C. burnetii phase II antigen, indicative of acute Q fever, were detected. (aaem.pl)
  • The Q fever agent Coxiella burnetii is a Gram-negative bacterium that invades macrophages and replicates inside a specialized lysosomal vacuole. (asm.org)
  • Coxiella burnetii is a Gram-negative intracellular pathogen that causes human Q fever, a zoonotic disease that is commonly transmitted to humans through inhalation of by-products generated by infected domestic livestock ( 1 ). (asm.org)
  • During a large Q fever outbreak, routine C. burnetii screening starting at 20 weeks of gestation was not associated with a relevant reduction in obstetric complications and should therefore not be recommended. (eurosurveillance.org)
  • Monocytic THP-1 cells were infected with Coxiella burnetii , the etiological agent of Q fever, and then treated with IFN-γ. (jimmunol.org)
  • The onset of each clinical form of Q fever does not depend on the specific phenotype of C. burnetii 3 , but is determined by the host immune response. (jimmunol.org)
  • van Roeden Sonja E , Holsboer Eleonoor W , Oosterheert Jan Jelrik , van Kats Jorge P , van Beckhoven Jacqueline , Hogema Boris M , van Wijk Marja J . Seroprevalence of Coxiella burnetii antibodies and chronic Q fever among post-mortal and living donors of tissues and cells from 2010 to 2015 in the Netherlands. (eurosurveillance.org)
  • We performed a retrospective observational cohort study among 15,133 Dutch donors of tissues and stem cells from 2010 to 2015 to assess seroprevalence of Coxiella burnetii antibodies, to identify factors associated with presence of C. burnetii antibodies, and to assess the proportion of undiagnosed chronic Q fever cases. (eurosurveillance.org)
  • Screening for Coxiella burnetii seroprevalence in chronic Q fever high-risk groups reveals the magnitude of the Dutch Q fever outbreak. (eurosurveillance.org)
  • From Q fever to Coxiella burnetii: a paradigm change. (eurosurveillance.org)
  • Q fever and Coxiella burnetii: a model for host-parasite interactions. (asm.org)
  • The obligate intracellular bacterium Coxiella burnetii , the agent of Q fever in humans and of coxiellosis in other animals, survives and replicates within large, acidified, phagolysosome-like vacuoles known to fuse homo- and heterotypically with other vesicles. (asm.org)
  • Coxiella burnetii is the causative agent of human Q fever, a zoonotic disease that can cause a debilitating, flu‐like illness in acute cases, or a life‐threatening endocarditis in chronic patients. (iupui.edu)
  • Coxiella burnetii is a Gram-negative, obligate intracellular bacterium and the causative agent of Q fever, a widespread zoonotic infectious disease ( 2 , 11 ). (asm.org)
  • Sixty percent of patients exposed to C. burnetii seroconvert asymptomatically, whereas 40% develop a disease known as acute Q fever, which in general presents as a mild flu-like disease. (asm.org)
  • Primary C. burnetii infection may progress to chronic Q fever, presenting as endocarditis or vascular disease in 78% and 9% of chronic Q fever patients, respectively ( 19 ). (asm.org)
  • Dairy cattle are a primary reservoir of Coxiella burnetii, which causes Q fever. (cdc.gov)
  • Coxiella burnetii is an intracellular bacterium that causes the disease Q fever, which presents in both acute and chronic forms in humans ( 1 ). (asm.org)
  • Q fever is transmitted by inhalation of contaminated aerosols, and infectious aerosols are typically contaminated with C. burnetii from the waste products of infected animals. (asm.org)
  • Goats are known reservoirs of Coxiella burnetii, the etiologic agent of Q fever. (biomedcentral.com)
  • Coxiella burnetii , the causative agent of Q fever, is a zoonotic pathogen associated with sylvatic or domestic transmission cycles, with rodents being suspected to link the two transmission cycles. (biomedcentral.com)
  • Coxiella burnetii is a zoonotic pathogen that resides in wild and domesticated animals across the globe and causes a febrile illness, Q fever, in humans. (biomedcentral.com)
  • Sporadic outbreaks of Q fever have occurred in the United Kingdom, but the local genetic make-up of C. burnetii has not been studied in detail. (biomedcentral.com)
  • Coxiella burnetii is an obligate intracellular pathogen and the etiological agent of Q-fever, a zoonotic disease of humans which has been reported from almost every country worldwide [ 1 ]. (biomedcentral.com)
  • In a previous study of a Q fever outbreak in Birmingham, our group identified a non-infective complex of Coxiella burnetii ( C.b. ) antigens able to survive in the host and provoked aberrant humoral and cell-mediated immunity responses. (biomedcentral.com)
  • Coxiella burnetii, a gram-negative obligate intracellular bacterium, causes human Q fever and is considered a potential agent of bioterrorism. (nih.gov)
  • Q-fever is an underreported disease caused by the bacterium Coxiella burnetii , which is highly infectious and has the ability to disperse great distances. (microbiologyresearch.org)
  • 1. Seroprevalence and risk factors of Coxiella burnetii (Q fever) infection in humans in Bura irrigation scheme, Tana River County, Kenya Mwololo D.K.,1, 3 Kitala P.M.,1 Wanyoike S.K.2 and Bett B.3 1. (slideshare.net)
  • 2. INTRODUCTION Coxiellosis (Q fever) is a zoonotic disease caused by the bacterium Coxiella burnetii (CB) (Brom et al. (slideshare.net)
  • Infections from Coxiella burnetii , resulting in what is known as Q fever, are relatively rare and difficult to diagnose. (scientificscholar.in)
  • Coxiella burnetii is a ubiquitous zoonotic bacterial pathogen and the cause of human acute Q fever, a disabling influenza-like illness. (grantome.com)
  • BACKGROUND: Prior to the 2007-2010 Q fever epidemic in the Netherlands, the seroprevalence of antibodies against Coxiella burnetii in the general population was 1.5%, which is low compared to other countries. (uu.nl)
  • the seroprevalence with the incidence of Q fever notifications during the 2007-2010 Q fever epidemic, and to identify farm exposures associated with having antibodies against C. burnetii. (uu.nl)
  • We compared the C. burnetii seroprevalence in the period March 2014-February 2015 with the incidence of Q fever notifications during the 2007-2010 Q fever epidemic at municipal level by calculating the Spearman correlation coefficient. (uu.nl)
  • There was no significant correlation between C. burnetii seroprevalence and Q fever incidence during the 2007-2010 epidemic (r s = 0.42, p = 0.156). (uu.nl)
  • It confirms previous evidence that the Q fever epidemic was primarily the result of airborne C. burnetii transmission from Q fever affected goat farms. (uu.nl)
  • Coxiella burnetii is an intracellular bacterial pathogen that causes human Q fever, an acute flu-like illness that can progress to chronic endocarditis and liver and bone infections. (elsevier.com)
  • PCR amplification has been used to detect C. burnetii DNA in clinical samples from acute and chronic Q fever patients. (microbenotes.com)
  • purpose: Cardiac valves that were resected from patients with Q fever endocarditis were examined by immunohistologic methods to correlate the presence of Coxiella burnetii in the valves with the histopathologic, serologic, microbiologic, and clinical findings. (elsevier.com)
  • Brouqui, P, Dumler, JS & Raoult, D 1994, ' Immunohistologic demonstration of coxiella burnetii in the valves of patients with Q fever endocarditis ', The American journal of medicine , vol. 97, no. 5, pp. 451-458. (elsevier.com)
  • Although prevalence of C. burnetii is high, few Q fever cases are reported in the U.S. and we have a limited understanding of their connectedness due to difficulties in genotyping. (edu.au)
  • Coxiella burnetii (C. burnetii) is an intracellular gram-negative bacterium, which is the causative agent of the widespread zoonotic disease Q fever. (edu.au)
  • Coxiella burnetii is the etiologic agent of human Q fever and targets alveolar phagocytic cells in vivo wherein the pathogen generates a phagolysosome-like parasitophorous vacuole (PV) for replication. (sparrho.com)
  • Coxiella burnetii is the causative agent of the zoonotic disease Q fever. (biomedcentral.com)
  • Q fever is a zoonotic disease caused by the bacterium Coxiella burnetii . (biomedcentral.com)
  • An easy-to-handle microarray assay based on the cost-effective ArrayTube™ platform has been designed for the rapid and unequivocal identification of Coxiella burnetii, the causative agent of Q fever. (biomedcentral.com)
  • Coxiella burnetii is a human pathogen that causes the serious zoonotic disease Q fever. (cdc.gov)
  • From 2007 through 2010, over 4,000 human cases were notified and approximately a twelve-fold higher number was probably infected by Coxiella burnetii, the causative agent of Q fever. (uu.nl)
  • Modelled airborne Coxiella burnetii concentrations were a better predictor for Q fever incidence than distance alone. (uu.nl)
  • 4) Several variables related to transmission through re-aerosolisation from a contaminated environment - such as the sensitivity of soils to wind erosion - increased the correlation to reported Q fever incidence rates and thus probably influenced Coxiella burnetii exposure. (uu.nl)
  • Q fever, which is caused by the bacterium Coxiella burnetii , has rapidly emerged in French Guiana since 1996 ( 1 ). (blogspot.com)
  • The most common clinical feature of Q fever in Cayenne is pneumonia, and C. burnetii is the causative agent of 24% of all community-acquired pneumonias ( 4 ). (blogspot.com)
  • Cultivation in ACCM-2 will accelerate development of C. burnetii genetic tools and provide a sensitive means of primary isolation of the pathogen from Q fever patients. (scienceexchange.com)
  • Coxiella burnetii is a Gram-negative pleomorphic bacterium and the causative agent of Q fever. (nebraska.edu)
  • As Q fever , caused by Coxiella burnetii, is a major health challenge due to its cardiovascular complications, we aim to detect acute Q fever valvular injury to improve therapeutic management.In the French national reference center for Q fever , we prospectively collected data from patients with acute Q fever and valvular injury. (tripdatabase.com)
  • acute Q fever endocarditis, defined as valvular lesion potentially caused by C. burnetii: vegetation, valvular nodular thickening, rupture of chorda tendinae and valve or chorda tendinae thickness. (tripdatabase.com)
  • Coxiella burnetii, the agent causing Q fever , has been associated with B-cell non-Hodgkin lymphoma (NHL). (tripdatabase.com)
  • Coxiella burnetii is a Gram-negative obligate intracellular bacterium that infects a wide range of mammalian species, and causes the disease syndrome Q fever. (beds.ac.uk)
  • Q fever is a zoonosis caused by the intracellular bacterium Coxiella burnetii . (biomedcentral.com)
  • We therefore inoculated nulliparous goats with C. burnetii , isolated from the outbreak of Q fever in the Netherlands, to gain a better understanding of the role of non-pregnant goats. (biomedcentral.com)
  • Q fever articular infections may be undiagnosed, and we strongly urge the use of positron emission tomography scanning in patients with high C. burnetii antibody titers to localize the site of C. burnetii infection. (biomedcentral.com)
  • Q fever or query fever is a disease caused by infection with Coxiella burnetii, a bacterium that affects humans and other animals. (wikipedia.org)
  • Background and Aim: In the course of our Indian Council of Medical Research project on coxiellosis in Puducherry and Tamil Nadu, 5.64% goat, 1.85% sheep, 1.06% buffaloes, and 0.97% cattle were positive for Coxiella burnetii antibodies by enzyme linked immunosorbent assay kit (IDEXX, Liebefeld, Switzerland). (doaj.org)
  • Prevalence of Coxiella burnetii antibodies in wild ruminants in Kavecany zoo, Kosice, eastern Slovakia. (aaem.pl)
  • Prevalence of antibodies to Coxiella burnetii among farming population in eastern Poland. (aaem.pl)
  • This study shows that seroprevalence of C. burnetii antibodies among donors of tissues and cells in the Netherlands after 2014 was similar to pre-outbreak levels in the general population. (eurosurveillance.org)
  • A recent seroprevalence study in the United States found that 3.1% of the adult population in the United States has antibodies against C. burnetii ( 4 ). (asm.org)
  • Banked serum samples were secondarily tested for C. burnetii specific antibodies. (biomedcentral.com)
  • abstract = "Milk samples of 12 Danish dairy herds were collected 3 times during an 11-mo period and tested for Coxiella burnetii DNA by real-time PCR, detecting the IS1111 element, and for the presence of antibodies against the bacterium by ELISA. (dtu.dk)
  • The prevalence levels of C. burnetii DNA and antibodies in the herds were found to be rather stable for 6 of the herds. (dtu.dk)
  • The antibody levels in bulk tank milk and prevalence levels of C. burnetii DNA and antibodies in individual cow milk samples were correlated. (dtu.dk)
  • Several studies using polyclonal and monoclonal antibodies revealed different binding patterns with LPS from different C. burnetii isolates [ 14 , 15 ]. (biomedcentral.com)
  • 9. SAMPLE ANALYSIS A commercial ELISA antibody test kit (SERION ELISA classic Coxiella burnetii Phase 1 IgG) (www.virion-serion.de) was used for the detection of human Coxiella burnetii antibodies in serum. (slideshare.net)
  • To compare ELISA-positive participants for C. burnetii antibodies with those who were negative, we calculated prevalence ratios (PR) using binominal regression. (uu.nl)
  • and pathologic and immunohistologic testing for localization of C burnetii in resected valves were performed by standard methods using both polyclonal and monoclonal C burnetii antibodies. (elsevier.com)
  • Immunogenicity tests are commonly used for detection of antibodies against C. burnetii in wildlife species. (edu.au)
  • There is, however, no validated ELISA test for detection of C. burnetii antibodies across Australian wildlife species. (edu.au)
  • Bulk tank milk (BTM) samples were collected from 81 sheep flocks in the Basque Country, Spain, in 2015 and were analysed for antibodies against Coxiella burnetii by ELISA and for C. burnetii DNA by real-time PCR. (biomedcentral.com)
  • Using antibodies against the C. burnetii T4BSS homologs IcmT, IcmV, and DotH, IFA show that these proteins are localized to the poles of the bacterium. (nebraska.edu)
  • IEM supports this finding, showing that antibodies against C. burnetii IcmT and DotH preferentially localize to the bacterial cell pole(s). (nebraska.edu)
  • Serum was examined for anti- C. burnetii antibodies by enzyme-linked immunosorbent assay (ELISA). (beds.ac.uk)
  • Serology revealed that 70% of the cattle were positive for antibodies to C. burnetii , while PCR analysis identified 25% of endocarditis valve samples as being positive. (beds.ac.uk)
  • National surveys of antibodies against C. burnetii were performed for dairy cattle (n=1537), dairy goats (n=58) and sheep (n=518). (biomedcentral.com)
  • The prevalence of antibodies against C. burnetii in dairy cattle in Sweden shows large regional differences. (biomedcentral.com)
  • Antibodies against C. burnetii are usually detected by enzyme-linked immunosorbent assays (ELISAs), indirect immunofluorescence (IFA) or by the complement fixation test (CFT). (biomedcentral.com)
  • Also, a comparison of two ELISAs for the detection of antibodies against C. burnetii in cattle, sheep and goats is reported. (biomedcentral.com)
  • The prevalence of antibodies to C. burnetii among dairy herds was determined in a national bulk milk survey conducted in November 2008 and in June 2009. (biomedcentral.com)
  • Coxiella burnetii is an obligate intracellular bacterial pathogen that replicates inside the lysosome-derived Coxiella -containing vacuole (CCV). (asm.org)
  • The pathogen persists in the environment as a metabolically quiescent small cell variant (SCV), which transforms into a metabolically active large cell variant (LCV) within a lysosome-derived, acidic (pH ~4.5), Coxiella -containing vacuole (CCV) ( Voth and Heinzen, 2007 ). (frontiersin.org)
  • The pathogen employs a type 4B secretion system (T4BSS) to deliver effector proteins into the host cell that modify the Coxiella- containing vacuole (CCV) into a replication-permissive niche. (asm.org)
  • Together, these data demonstrate that specific inhibition of mTORC1 by C. burnetii , but not amplified cell catabolism via autophagy, is required for optimal pathogen replication. (asm.org)
  • The obligate intracellular pathogen Coxiella burnetii has long been considered the most heat resistant pathogen in raw milk, making it the reference pathogen for determining pasteurisation conditions for milk products. (unboundmedicine.com)
  • An obligate intracellular bacterial pathogen, Coxiella survives and multiplies in a large lysosome‐like vacuole known as the Coxiella parasitophorous vacuole (CPV). (iupui.edu)
  • Using pathogen-specific polymerase chain reaction assays targeting the 16S rRNA gene, C. burnetii was detected for the first time in 45% of rodents (9/20), in one shrew and in 10% of domestic dogs (15/150) screened in Zambia. (biomedcentral.com)
  • This study aimed to detect and characterize C. burnetii in rodents and semi-confined domestic dogs from rural and peri-urban settings, respectively, to ascertain the potential role of these animal hosts in the epidemiological cycle of this pathogen. (biomedcentral.com)
  • As a species, C. burnetii has been considered a clonal pathogen with low genetic diversity at the nucleotide level. (biomedcentral.com)
  • Our results thereby increase our understanding of the global genetic diversity of C. burnetii and provide new insights into the evolution of this emerging zoonotic pathogen. (biomedcentral.com)
  • Using Himar1, we have generated mCherry and GFP-expressing Coxiella useful for host-pathogen interaction studies. (grantome.com)
  • Humans are typically infected by aerosol-mediated transmission, and C. burnetii initially targets alveolar macrophages wherein the pathogen replicates in a phagolysosome-like niche known as the parasitophorous vacuole (PV). (elsevier.com)
  • As it is an intracellular pathogen, infection by C. burnetii requires adaptation to its eukaryotic host and intracellular environment. (biomedcentral.com)
  • Here, we describe modified ACCM and culture conditions that support improved growth of C. burnetii and their use in genetic transformation and pathogen isolation from tissue samples. (scienceexchange.com)
  • The results suggest that C. burnetii is a rare pathogen among Swedish moose, dairy goat and sheep. (biomedcentral.com)
  • An acidified citrate cysteine medium (ACCM-2) has been developed which mimics the intracellular replicative niche of C. burnetii and allows axenic growth of the bacteria. (asm.org)
  • Recent studies indicate that C. burnetii evolved from a tick-associated ancestor and that the metabolic capabilities of C. burnetii are different from that of Coxiella -like bacteria found in ticks. (frontiersin.org)
  • The data reveal that in response to C. burnetii , MCs release extracellular actin filaments that contain antimicrobial agents and are capable to trap and kill bacteria. (asm.org)
  • Overall, this study reports an unexpected role for MCs during infection with intracellular bacteria and suggests that MC response to C. burnetii infection is a protective defense mechanism during pregnancy. (asm.org)
  • In contrast, Coxiella burnetii bacteria live and replicate in acidified compartments with phagolysosomal characteristics ( 23 ). (asm.org)
  • Ruminant livestock are common reservoirs for C. burnetii , and bacteria present in aerosols derived from the waste of infected animals can infect humans. (asm.org)
  • There is evidence that C. burnetii is a public health hazard in the US with humans being exposed to the bacteria through milk. (biomedcentral.com)
  • As observed in other gram-negative bacteria, Tn7 inserts with high frequency into a single intergenic site downstream from Coxiella glmS encoding glucosamine-6-phophosphate synthetase. (grantome.com)
  • The PhoBR two-component system of Coxiella is especially intriguing as homologous systems in other bacteria regulate virulence gene expression. (grantome.com)
  • As an amenable model to help better understand the biological relevance of Coxiella differentiation, we established that SCV/LCV transitions are recapitulated by organisms growing in the third-generation axenic media, ACCM-D. This discovery enables studies of Coxiella developmental biology without experimental difficulties encountered with host cell-propagated bacteria. (grantome.com)
  • However, with the discovery and study of Coxiella-like bacteria (CLB), many PCR genes targeted in routine molecular detection assays were found to also be present in CLB. (edu.au)
  • Histologically, bacteria other than C. burnetii were observed in most cases. (beds.ac.uk)
  • However, some bacteria, such as C. burnetii, cannot be cultured by standard bacteriological methods as the bacterium requires cell cultures for propagation due to its intracellular nature. (beds.ac.uk)
  • The Coxiella burnetii parasitophorous vacuole. (nih.gov)
  • Coxiella burnetii is a bacterial intracellular parasite of eucaryotic cells that replicates within a membrane-bound compartment, or "parasitophorous vacuole" (PV). (nih.gov)
  • Fusogenicity of the Coxiella burnetii parasitophorous vacuole. (nih.gov)
  • This study investigated whether C. burnetii protein synthesis and replication is required for maintenance of the fusogenic character of the Coxiella parasitophorous vacuole (PV). (nih.gov)
  • C. burnetii is a Gram-negative intracellular bacterium that replicates within an acidic lysosome-like parasitophorous vacuole (PV) in human macrophages. (asm.org)
  • C. burnetii resists the degradative functions of the vacuole while at the same time exploiting the acidic pH for metabolic activation. (nih.gov)
  • Successful host cell infection requires the Coxiella type IVB secretion system (T4BSS), which translocates bacterial effector proteins across the vacuole membrane into the host cytoplasm, where they. (asm.org)
  • IMPORTANCE Coxiella burnetii is an intracellular pathogenic bacterium that replicates within a lysosomal vacuole. (asm.org)
  • Biogenesis of the Coxiella -containing vacuole (CCV) requires effector proteins delivered into the host cell cytosol by the type 4B secretion system (T4BSS). (asm.org)
  • C. burnetii colonizes alveolar macrophages, where it occupies a vacuole that matures canonically within the endosomal pathway, culminating in lysosome fusion ( 2 ). (asm.org)
  • By mechanisms not fully understood, C. burnetii resists degradation by acid hydrolases delivered by ongoing fusion of lysosomes with the Coxiella -containing vacuole (CCV). (asm.org)
  • Furthermore, 3-methyladenine and wortmannin, agents known to inhibit early stages in the autophagic process, each blocked Coxiella vacuole formation. (asm.org)
  • Prevalence of Coxiella burnetii infections among North Dakota sheep producers. (cdc.gov)
  • To estimate the prevalence of Coxiella burnetii infection and identify associated risk factors, we conducted a study covering the whole state. (cdc.gov)
  • Prevalence of Coxiella burnetii and Brucella spp. (biomedcentral.com)
  • A study based on bulk tank milk samples from 120 randomly selected dairy cattle herds was conducted to estimate the prevalence of Coxiella burnetii seropositive dairy herds, to describe the geographical distribut. (biomedcentral.com)
  • Muskens J, Van Engelen E, Van Maanen C, Bartels C, Lam T. Prevalence of Coxiella burnetii infection in Dutch dairy herds based on testing bulk tank milk and individual samples by PCR and ELISA. (ac.ir)
  • There is also evidence of antigenic variation between C. burnetii isolates, which includes both the O-antigen of the lipopolysaccharide (LPS) as well as antigenic proteins [ 13 ]. (biomedcentral.com)
  • Here we comprehensively define the genetic diversity of C. burnetii by hybridizing the genomes of 20 RFLP-grouped and four ungrouped isolates from disparate sources to a high-density custom Affymetrix GeneChip containing all open reading frames (ORFs) of the Nine Mile phase I (NMI) reference isolate. (nih.gov)
  • A total of 139 chromosomal and plasmid ORFs were polymorphic among all C. burnetii isolates, representing ca. 7% of the NMI coding capacity. (nih.gov)
  • Comparison of the genomes of 22 C. burnetii isolates with the genome of the. (nih.gov)
  • Molecular characterization of Coxiella burnetii isolates by infrequent restriction site-PCR and MLVA typing. (microbiologyresearch.org)
  • The high passage phase II isolates in our stock collection are not clonal and contain a small subpopulation of Coxiella still expressing full-length phase I LPS. (grantome.com)
  • In the event of an outbreak or intentional release, laboratory strain typing methods can contribute to epidemiological investigations, law enforcement investigation and the public health response by providing critical information about the relatedness between C. burnetii isolates collected from different sources. (cdc.gov)
  • We obtained 5 Coxiella burnetii isolates from samples of patients from Cayenne and found an epidemic clone circulating in Cayenne. (blogspot.com)
  • Similar growth was achieved with the virulent Nine Mile phase I and G isolates of C. burnetii. (scienceexchange.com)
  • Also, in scientific reports submitted to the European Food Safety Authority (EFSA) ELISAs using C. burnetii antigens prepared from ruminant isolates are recommended [ 11 , 12 ]. (biomedcentral.com)
  • The causes of the Dutch outbreak were not well-understood and one of the main reasons speculated involved potential hyper-virulence of C. burnetii outbreak strains. (wur.nl)
  • The results from this thesis research suggested that virulence potential of C. burnetii strains was mediated by genetic differences between strains associated with mobility of genetic elements and/or differential regulation of gene expression. (wur.nl)
  • Our analyses identified a large number of putative foreign-origin genes in C. burnetii , including tRNA Glu 2 that is potentially required for heme biosynthesis, and genes involved in the production of lipopolysaccharide-a virulence factor, and of critical metabolites such as fatty acids and biotin. (frontiersin.org)
  • A unique characteristic of the CPV is the acidic environment (pH ∼5.0), which is required to activate Coxiella metabolism and the Coxiella type 4 secretion system (T4SS), a major virulence factor required for intracellular survival. (iupui.edu)
  • Together, these data suggest that CPV pH is an important limiting factor for Coxiella growth and virulence. (iupui.edu)
  • An improved understanding of the genetic diversity of C. burnetii and its virulence mechanisms is essential for the development of diagnostics, vaccines and therapeutics. (biomedcentral.com)
  • Very few virulence-associated genes are annotated and virulence mechanisms of C. burnetii are still poorly understood. (biomedcentral.com)
  • Our results provide new insight into the genetic diversity and virulence potential of Coxiella species. (nih.gov)
  • Collectively, our repertoire of Coxiella genetic tools now allows traditional mutation and complementation strategies for virulence factor discovery. (grantome.com)
  • The only genetic lesions proven to result in attenuated Coxiella virulence in an immunocompetent animal model are associated with defective LPS synthesis. (grantome.com)
  • Given the critical role of LPS in Coxiella virulence, it is important to understand the molecular basis of phase variation. (grantome.com)
  • We studied C. burnetii genome-wide transcriptional patterns in vivo (mice spleen) and in cell and cell-free in vitro culture models to examine its metabolic pathways and virulence associated gene expression patterns that are required to colonize and persist in different environments. (biomedcentral.com)
  • T4BSSs have only been described in L. pneumophila and C. burnetii, marking it a unique virulence determinate. (nebraska.edu)
  • The first description of what may have been Coxiella burnetii was published in 1925 by Hideyo Noguchi, but since his samples did not survive, it remains unclear as to whether it was the same organism. (wikipedia.org)
  • Around the same time, Derrick proposed the name Rickettsia burnetii, in recognition of Burnet's contribution in identifying the organism as a Rickettsia. (wikipedia.org)
  • C burnetii is highly infectious, and a single organism can reportedly cause infection via the aerosol route in people. (colorado.gov)
  • A negative result does not negate the presence of the organism or active disease, as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of Coxiella burnetii in quantities less than the limit of detection of the assay. (mayomedicallaboratories.com)
  • The lack of methods to genetically manipulate Coxiella significantly impedes study of the organism. (grantome.com)
  • In humans, infection with Coxiella burnetii can cause endocarditis, but this has not been investigated thoroughly in animals. (beds.ac.uk)
  • Despite exposure of cattle to C. burnetii , the significance of this particular bacterium for development and progression of endocarditis has not been studied in detail. (beds.ac.uk)
  • The presence of C. burnetii DNA is relatively common in cattle affected with valvular endocarditis. (beds.ac.uk)
  • Based on the comparative aspects in humans, where C. burnetii is a well-known cause of endocarditis, it could be suspected that C. burnetii may also be implicated in the development or progression of endocarditis in cattle under certain circumstances. (beds.ac.uk)
  • Vero cells were infected with C. burnetii, (Nine Mile strain in phase II) at a multiplicity of infection of approximately 10 and simultaneously treated with bacteriostatic concentrations of chloramphenicol or carbenicillin. (nih.gov)
  • In comparison to wild-type C. burnetii , a strain that lacks tRNA Glu 2 exhibited reduced growth, indicating its importance to Coxiella 's physiology. (frontiersin.org)
  • Although the presence of a hypervirulent strain is hypothesized, epidemiological evidence, such as the animal reservoir(s) and genotype of the C. burnetii strain(s) involved, is still lacking. (asm.org)
  • Within each model, the gene expression profiles of the Dutch C. burnetii outbreak strain (602) and NM reference strains were largely similar. (biomedcentral.com)
  • Two strains of C. burnetii, our isolate from TOL cells and the original isolate in 1935, the Nine Mile strain from American Type Culture Collection (ATCC, U.S.A), were inoculated to PBL cultures. (ndsl.kr)
  • Building upon epidemiological knowledge from limited, historical strain collections and typing data is essential to more accurately infer C. burnetii phylogeny. (cdc.gov)
  • Subsequently, we have hypothesized the existence of a specific source of C. burnetii responsible for human infections, which is unidentified to date, and the existence of a different strain of C. burnetii that circulates in Cayenne. (blogspot.com)
  • Analysis of the C. burnetii (RSA 493 strain) genome sequence indicates that C. burnetii contains genes with homology to the Legionella pneumophila Dot/Icm type IVB secretion system (T4BSS). (nebraska.edu)
  • This thesis research focused on the characterization of C. burnetii outbreak strains isolated from infected goats, cattle, sheep and clinical human samples. (wur.nl)
  • In spite of a high degree of IVS conservation among different strains of C. burnetii , the region immediately upstream of the S23p start codon is prone to change, and the S23p-encoding ORF is evidently undergoing reductive evolution. (frontiersin.org)
  • SNP genotyping appeared to be highly suitable for discrimination of C. burnetii strains and easy to perform with clinical samples. (asm.org)
  • Indeed, we have constructed knockout strains in both virulent and avirulent Coxiella, including those with deletions in genes encoding components of the Dot/Icm type IVB secretion system (T4BSS) and secreted proteins. (grantome.com)
  • Thirteen strains within the six major genetic groups of Coxiella were evaluated for pathogenicity in a guinea pig model of infection. (grantome.com)
  • Strains of C. burnetii differ in their plasmids which they carry. (microbenotes.com)
  • In this study, we isolated 5 C. burnetii strains from biologic samples of patients from Cayenne. (blogspot.com)
  • Coxiella brunetii has a broad host range but is detected primarily in sheep, goats, cattle and wild ruminants. (bund.de)
  • Further testing revealed C burnetii in additional goats, cows, and yaks on the farm. (colorado.gov)
  • Unadjusted odds ratios showed that governorate of residence, consumption of raw milk, and ownership of sheep, goats, and dogs were significantly ( P ≤ 0.05) associated with C. burnetii seropositivity. (ajtmh.org)
  • However, there has been very little research on the prevalence of C. burnetii exposure and risk in meat goats farmed in the US. (biomedcentral.com)
  • This study indicates that seroprevalence of C. burnetii in Boer goats raised in Missouri was low, but it does not preclude the existence of a higher level of infection in Missouri's meat goat herds. (biomedcentral.com)
  • No studies have evaluated the prevalence and risk factors of C. burnetii infections in meat goats farmed in Missouri. (biomedcentral.com)
  • The aim of this pilot investigation was to determine whether Boer goats, the preponderant meat goat breed in Missouri, were exposed to C. burnetii and to estimate seroprevalence of the exposure. (biomedcentral.com)
  • Animals infected by C. burnetii often show no clinical signs, although some may develop reproductive problems such as stillbirth and abortion in cattle, sheep, and goats. (edu.au)
  • To study the effect of breeding on the excretion of C. burnetii , the goats were naturally bred and monitored during gestation and after lambing. (biomedcentral.com)
  • Therefore, the goal of this study was to assess C. burnetii infection and (milk) excretion in non-pregnant nulliparous goats up to the outcome of the first pregnancy and start of lactation. (biomedcentral.com)
  • Goats were synchronized and bred, and after parturition, placenta's, kids, mammary glands, and colostrum were investigated by C. burnetii -specific PCR. (biomedcentral.com)
  • The aim was to investigate the presence of or exposure to C. burnetii in cattle, sheep, goats and moose, and to compare two enzyme-linked immunosorbent assays (ELISAs). (biomedcentral.com)
  • This study presents a series of investigations into the presence of C. burnetii in Swedish cattle, sheep, goats and moose. (biomedcentral.com)
  • To survive in macrophages, Coxiella burnetii hijacks the activation pathway of macrophages. (frontiersin.org)
  • In macrophages challenged with LPS from the avirulent variants of C. burnetii , TLR-4 and TLR-2 co-immunoprecipitated. (frontiersin.org)
  • These studies have confirmed that T4BSS function is critical for Coxiella growth in macrophages. (grantome.com)
  • Avirulent and virulent C. burnetii triggered increased levels of phosphorylated VASP in macrophage-like THP-1 cells and primary human alveolar macrophages, and this event required the Cα subunit of PKA. (elsevier.com)
  • C burnetii were present nearly exclusively in macrophages in sites of inflammation and valvular injury and only in the vegetations. (elsevier.com)
  • Coxiella burnetii alters cyclic AMP-dependent protein kinase signaling during growth in macrophages. (sparrho.com)
  • Cloning and characterization of an autonomous replication sequence from Coxiella burnetii. (semanticscholar.org)
  • Characterization of a Coxiella burnetii ftsZ mutant generated by Himar1 transposon mutagenesis. (semanticscholar.org)
  • This disease is created by an obligate intracellular Rickettsia called Coxiella burnetii (C. burnetii) . (ac.ir)
  • The genus Coxiella is morphologically similar to Rickettsia, but with a variety of genetic and physiological differences. (wikipedia.org)
  • As it became clear that the species differed significantly from other Rickettsia, it was first elevated to a subgenus named after Cox, Coxiella, and then in 1948 to its own genus of that name, proposed by Cornelius B. Philip, another RML researcher. (wikipedia.org)
  • Borrelia burgdorferi sensu lato, Coxiella burnetii, and Rickettsia spp. (google.com)
  • Coxiella burnetii, and Rickettsia spp. (google.com)
  • [1] X Trustworthy Source Centers for Disease Control and Prevention Main public health institute for the US, run by the Dept. of Health and Human Services Go to source Unlike many other bacterial infections, Coxiella burnetii is resistant to heat and to dry climate, and also shows resistance to common household disinfectants. (wikihow.com)
  • Most human infections occur through inhalation of aerosols originating from ruminants that shed C. burnetii during parturition and in milk. (frontiersin.org)
  • Occupationally exposed persons may reduce their risk of infection with burnetii by wearing respirators that prevent aerosol infections. (microbenotes.com)
  • sheep farms, were identified as the major source of these human infections with high Coxiella burnetii shedding rates during parturition of the animals. (uu.nl)
  • Overall, field data and data from experimental infections are contradictory and do not explain how a C. burnetii infection is maintained in a herd. (biomedcentral.com)
  • This data will be used in future investigations, in which we will combine molecular detection and typing methods for C. burnetii in aerosols with mathematical modelling to get more insight in the transmission of C. burnetii via aerosols and track (individual) sources for C. burnetii infection. (rivm.nl)
  • Immunodetection and molecular detection are commonly used to diagnose infection with C. burnetii. (edu.au)
  • Findings from this project address the importance of appropriate molecular methods used for the detection of C. burnetii. (edu.au)
  • This result suggests that de novo C. burnetii protein synthesis, but not replication, is required for homotypic fusion and maturation of nascent C. burnetii PV. (nih.gov)
  • however, because of its obligate intracellular replication, C. burnetii would have lost most genes that have been rendered redundant due to the availability of metabolites within the host cell. (frontiersin.org)
  • The acid environment appears to be essential for C. burnetii replication since raising the lysosomal pH with lysosomotropic amines or proton pump V-ATPase inhibitors reduces the growth of C. burnetii ( 22 , 24 ). (asm.org)
  • A Coxiella burnetii chromosomal fragment capable of functioning as an origin for the replication of a kanamycin resistance (Kanr) plasmid was isolated by use of origin search methods utilizing an Escherichia coli host. (semanticscholar.org)
  • C. burnetii manipulates host cAMP-dependent protein kinase (PKA) signaling to promote PV formation, cell survival, and bacterial replication. (elsevier.com)
  • Coxiella burnetii exploits host cAMP-dependent protein kinase signalling to promote macrophage survival. (sparrho.com)
  • Evaluation of infected tissue, blood, or serum using PCR may be a useful tool for diagnosing some cases of Coxiella burnetii infection. (mayomedicallaboratories.com)
  • Results: A total of 15 antibody positive and three antibody-negative serum samples belonging to 11 goat, 4 sheep, 1 cattle, and 2 buffaloes were tested in duplicate for the presence of C. burnetii DNA by the commercial agar gel PCR kit and an in-house Trans-PCR. (doaj.org)
  • Only one buffalo serum sample was positive for C. burnetii with a band at 243 bp in in-house Trans-PCR. (doaj.org)
  • We have now developed a serum-free version of ACCM termed ACCM-2 that supports improved Coxiella growth in both liquid and solid media. (grantome.com)
  • Immunogenicity tests were applied to three antigen candidates using C. burnetii positive and negative goat serum samples by Western blotting and indirect ELISA. (edu.au)
  • Clinical evaluation of a new PCR assay for detection of Coxiella burnetii in human serum samples. (ac.ir)
  • An immunofluorescence assay of an acute serum sample was positive for C. burnetii and he was treated with 200 mg doxycycline for 21 days. (biomedcentral.com)
  • An immunofluorescence assay of convalescent serum sampled after 2 months revealed very high C. burnetii antibody titers. (biomedcentral.com)
  • Since asymptomatic Coxiella burnetii infection has been associated with maternal and obstetric complications, evidence about the effectiveness of routine screening during pregnancy in outbreak areas is needed. (eurosurveillance.org)
  • With this new method, we show that the Dutch outbreak is caused by at least 5 different C. burnetii genotypes. (asm.org)
  • SNP genotyping data from clinical samples clearly demonstrate that at least 5 different C. burnetii genotypes are involved in the Dutch outbreak. (asm.org)
  • Soil, vacuum, and sponge samples collected on seven farms epidemiologically linked to the outbreak were tested for the presence of C. burnetii DNA by quantitative PCR. (asm.org)
  • Follow-up sampling at one of the farms 1 year after the outbreak found small quantities of C. burnetii DNA in air samples and large quantities of C. burnetii persisting in soil and vacuum samples. (asm.org)
  • C burnetii is a zoonotic bacterial infection associated primarily with parturient ruminants although domestic animals, such as cats and a variety of wild animals, have been identified as sources of infection. (colorado.gov)
  • Reports have implicated C burnetii as a cause of infertility and sporadic abortion with necrotizing placentitis in ruminants. (colorado.gov)
  • Conversely, seronegative ruminants can shed C. burnetii. (doaj.org)
  • While infected ruminants remain largely asymptomatic, the primary signs of C. burnetii include spontaneous, late-term abortions in pregnant animals. (biomedcentral.com)
  • Genotyping of Coxiella burnetii from domestic ruminants in northern Spain. (microbiologyresearch.org)
  • The presence of C. burnetii in the Swedish goat population had not been investigated nor have studies been performed in wild ruminants. (biomedcentral.com)
  • Based on these observations, we reasoned that horizontally derived biosynthetic genes that have been retained in the reduced genome of C. burnetii are ideal candidates to begin to uncover its intracellular metabolic requirements. (frontiersin.org)
  • In spite of its intracellular nature, where chances for horizontal gene transfer are minimum, the genome of C. burnetii contains 31 insertion sequence (IS) elements spread across its genome, an intein in the C-terminal region of the replicative DNA helicase (DnaB) and two self-splicing group I introns (Cbu_L1917 and Cbu_L1951) along with an IVS in the 23S rRNA gene ( Minnick and Raghavan, 2011 ). (frontiersin.org)
  • Viability of the Coxiella in milk was shown by a ≥ 0.5 log genome equivalent (ge)/ml increase in the quantity of IS111a gene from the baseline post-infection (day 0) level after 9-11 d propagation. (unboundmedicine.com)
  • Here, we report whole genome data for nine C. burnetii sequences obtained in the UK. (biomedcentral.com)
  • All four genomes of C. burnetii from cattle, as well as one sheep sample, belonged to Multi-spacer sequence type (MST) 20, whereas the goat samples were MST33 (three genomes) and MST32 (one genome), two genotypes that have not been described to be present in the UK to date. (biomedcentral.com)
  • Coxiella plates with high efficiency on ACCM-2 agarose ( 1 to 2 genome equivalents per colony) and the resulting contain clonal populations. (grantome.com)
  • The adaptation to different hosts or extracellular environments has been assumed to involve genome-wide modulation of C. burnetii gene expression. (biomedcentral.com)
  • Genome-wide expression profiles of C. burnetii from in vitro cell culture were more similar to those seen for in vivo conditions, while gene expression profiles of cell-free culture were more distant to in vivo. (biomedcentral.com)
  • Our study shows that C. burnetii, with its relative small genome, requires only a subset of core gene functions to survive under in vitro conditions, but requires the induction of full repertoire of genes for successful pathogenesis and thriving in harsh environments in vivo. (biomedcentral.com)
  • This result is inconclusive because this study was disadvantaged by the small number of individual animal and herds tested, which compromised the statistical power of this study to detect a possible higher seroprevalence of C. burnetii in this population, if present. (biomedcentral.com)
  • The aim was to identify risk factors associated with Coxiella burnetii antibody positivity in bulk tank milk (BTM) samples from 100 randomly selected Danish dairy cattle herds. (biomedcentral.com)
  • Rahimi E, Doosti A, Ameri M, Kabiri E, Sharifian B. Detection of Coxiella burnetii by nested PCR in bulk milk samples from dairy bovine, ovine, and caprine herds in Iran. (ac.ir)
  • C. burnetii is a small obligate intracellular Gram-negative bacterium related to the Rickettsiaceae family. (biomedsearch.com)
  • C burnetii is common in livestock and animal testing does have limitations, as shedding can be intermittent. (colorado.gov)
  • CONCLUSIONS: Results showed a remarkable spatial variation in C. burnetii seroprevalence in a relatively small livestock dense area. (uu.nl)
  • Livestock are a significant source of human infection as they shed C. burnetii cells in birth tissues, milk, urine and feces. (edu.au)
  • The main conclusions of this work include: 1) Livestock-related sources of Coxiella burnetii could be identified, even in the early stages of local outbreaks assuming an exponential incidence-distance method. (uu.nl)
  • Taken together, our results suggest that MCs use an extracellular sophisticated mechanism of defense to eliminate intracellular pathogens, such as C. burnetii , before their entry into host cells. (asm.org)
  • Coxiella burnetii is excreted in the milk, urine, and feces of infected animals. (wikihow.com)
  • New milk formulations and novel non-thermal processes require validation of effectiveness which requires a more practical method for analysis than using the currently used animal model for assessing Coxiella survival. (unboundmedicine.com)
  • Also, there is an interest in better characterising thermal inactivation of Coxiella in various milk formulations. (unboundmedicine.com)
  • To avoid the use of the guinea pig model for evaluating Coxiella survival, an Integrated Cell Culture-PCR (ICC-PCR) method was developed for determining Coxiella viability in milk. (unboundmedicine.com)
  • Vero cell cultures were directly infected from Coxiella-contaminated milk in duplicate 24-well plates. (unboundmedicine.com)
  • Coxiella in skim, 2%, and whole milk, and half and half successfully infected Vero cells and increased in number by at least 2 logs using a 48-h infection period followed by 9-d propagation time. (unboundmedicine.com)
  • As few as 125 Coxiella ge/ml in whole milk was shown to infect and propagate at least 2 logs in the optimised ICC-PCR assay, though variable confirmation of propagation was shown for as low as 25 Coxiella ge/ml. (unboundmedicine.com)
  • Applicability of the ICC-PCR method was further proven in an MPN format to quantitate the number of viable Coxiella remaining in whole milk after 60 °C thermal treatment at 0, 20, 40, 60 and 90 min. (unboundmedicine.com)
  • TY - JOUR T1 - Quantitation of viable Coxiella burnetii in milk using an integrated cell culture-polymerase chain reaction (ICC-PCR) assay. (unboundmedicine.com)
  • A multivariable analysis identified a significant increase in C. burnetii DNA shedding with increasing parity and increasing protein concentration in milk. (dtu.dk)
  • A significant correlation was also found between the quantification cycle values of the cow samples (weighted according to milk yield) and the C. burnetii concentration in bulk tank milk. (dtu.dk)
  • C. burnetii , and in particular one genotype (ST20), is commonly found in cow's milk across the entire dairy industry of the USA. (microbiologyresearch.org)
  • Estimated herd prevalence and sequence types of Coxiella burnetii in bulk tank milk samples from commercial dairies in Indiana. (microbiologyresearch.org)
  • The bacterium Coxiella burnetii has been detected in the fetal membranes, birth fluids and vaginal mucus, as well as in the milk and other excretions of several domestic mammals. (biomedcentral.com)
  • This study showed that 20 samples (8.4%) of 238 non-pasteurized dairy products were reported positive for C. burnetii (13 of 48 (27.08%) raw milk, 3 of 48 (6.25%) yogurt, 2 of 46 (4.35%) cheese, 2 of 48 (4.16%) dough and 0 of 48 ice cream samples). (ac.ir)
  • Cerf O, Condron R. Coxiella burnetii and milk pasteurization: an early application of the precautionary principle? (ac.ir)
  • Investigation of Coxiella burnetii contamination in commercial milk and PCR method for the detection of C. burnetii in egg. (ac.ir)
  • Öngör H, Cetinkaya B, Karahan M, Açik MN, Bulut H, Muz A. Detection of Coxiella burnetii by immunomagnetic separation-PCR in the milk of sheep in Turkey. (ac.ir)
  • Detection of Coxiella burnetii in commercially available raw milk from the United States. (ac.ir)
  • Jamshidi A, Razmyar J, Borji S. Identification of Coxiella burnetii by touch-down PCR assay in unpasteurized milk and dairy products in North-East of Iran. (ac.ir)
  • Kazemi Kheirabadi E, Behzadnia A, Rahimi E. Coxiella burnetii in traditional raw milk, cheese and ice cream in Iran. (ac.ir)
  • Published trials proved that use of a registered phase vaccine (Coxevac) on infected farms is a tool of major interest to manage or prevent early or late abortion, repeat breeding, anoestrus, silent oestrus, metritis, and decreases in milk yield when C. burnetii is the major cause of these problems. (wikipedia.org)
  • Heart valves of normal cattle should be investigated to assess if C. burnetii may be present without preexisting lesions. (beds.ac.uk)
  • A total of 781 individuals from 11 governorates of Jordan were tested by SERION ELISA classic C. burnetii IgG Phase 2. (ajtmh.org)
  • Has serologic evidence of elevated phase II IgG or immunoglobulin M (IgM) antibody reactive with C. burnetii antigen by enzyme-linked immunosorbent assay (ELISA), dot-ELISA, or latex agglutination. (cdc.gov)
  • Three out of the four C. burnetii proteins selected (CBU0109, CBU0612, CBU0891, and CBU1910) as antigen candidates for ELISA development were expressed and purified. (edu.au)
  • The results revealed that none of the antigen candidates were suitable for ELISA development as all reacted with C. burnetii negative goat sera. (edu.au)
  • however, very little is known about genes that facilitate Coxiella 's intracellular growth. (frontiersin.org)
  • These data reveal a complex interplay between lysosomal function and host cell metabolism that regulates C. burnetii intracellular growth. (asm.org)
  • C. burnetii experienced nutrient limitation and anaerobiosis as major stressors, while phosphate limitation was identified as an important signal for intracellular growth inside eukaryotic host cells. (biomedcentral.com)
  • 40 tick species can be infected with C. burnetii, direct transmission of this agent to humans from infected ticks has never been properly documented. (cdc.gov)
  • Considering the close proximity of domestic dogs and rodents to humans, especially in resource-limited communities, the presence of C. burnetii in these animals could be of significant public health importance. (biomedcentral.com)
  • The current project was based on these two pilot studies and aimed at (1) modelling the airborne dispersion of Coxiella burnetii in the environment with a focus on farm-to-human transmission, and (2) identifying environmental risk factors for the transmission of Coxiella burnetii from infected farms to humans. (uu.nl)
  • 1. Frangoulidis D, Meyer H, Kahlhofer C, Splettstoesser WD: 'Real-time' PCR-based detection of Coxiella burnetii using conventional techniques. (mayomedicallaboratories.com)
  • Coxiella burnetii prosthetic joint infection-case report and assay for detection. (mayomedicallaboratories.com)
  • Berri M, Arricau-Bouvery N, Rodolakis A. PCR-based detection of Coxiella burnetii from clinical samples. (ac.ir)
  • Guatteo R, Beaudeau F, Berri M, Rodolakis A, Joly A, Seegers H. Shedding routes of Coxiella burnetii in dairy cows: implications for detection and control. (ac.ir)
  • This is an extremely low infectious dose (only 1-10 organisms required), making C. burnetii one of the most infectious known organisms. (wikipedia.org)
  • Coxiella burnetii is a highly infectious bacterium and potential agent of bioterrorism. (rcsb.org)
  • The number of sheep raised was a good predictor of C. burnetii infection. (cdc.gov)
  • We conclude that C. burnetii infection is prevalent among sheep producers in North Dakota. (cdc.gov)
  • The presence of C. burnetii in domestic animal populations in Sweden is known since the early 1990's, when the bacterium was first isolated from a sheep placenta [ 7 ]. (biomedcentral.com)
  • Physical and genetic map of the obligate intracellular bacterium Coxiella burnetii. (semanticscholar.org)
  • An improved understanding of the genetic diversity of C. burnetii is essential for the development of diagnostics, vaccines and therapeutics, but genotyping data is lacking from many parts of the world. (biomedcentral.com)
  • The genetic make-up of C. burnetii in the UK is similar to the one in neighboring European countries. (biomedcentral.com)
  • ACCM-2 will facilitate biochemical studies and, as described below, aid development of Coxiella genetic systems. (grantome.com)
  • 2) Development of new tools for Coxiella genetic manipulation. (grantome.com)
  • We found that Coxiella genetic diversity correlates with disease pathogenesis. (grantome.com)
  • Isolation from animal tissue and genetic transformation of Coxiella burnetii are facilitated by an improved axenic growth medium. (scienceexchange.com)
  • A completely axenic C. burnetii genetic transformation system was developed using ACCM-2 that allowed isolation of transformants in about 2 1/2 weeks. (scienceexchange.com)
  • To better clarify this link, we analysed the genetic transcriptomic profile of peripheral blood leukocytes from patients with C. burnetii infection to identify possible links to lymphoma. (tripdatabase.com)
  • In this chapter, we review current knowledge of PV nature and development, and discuss disparate data related to the ultimate maturation state of PV harboring virulent or avirulent C. burnetii lipopolysaccharide phase variants in human mononuclear phagocytes. (nih.gov)
  • Maximum parsimony phylogeny of 35 MST genotypes for Coxiella burnetii . (cdc.gov)
  • Reactive oxygen intermediates were not required for C. burnetii killing, since monocytes from patients with chronic granulomatous disease were microbicidal in response to IFN-γ. (jimmunol.org)
  • Only a few studies, mostly related to human clinical cases or seroepidemiogic evaluation of healthy animals, have reported C. burnetii in South America ( 2 - 4 ). (cdc.gov)
  • Mayo Medical Laboratories has developed a real-time PCR test that rapidly detects Coxiella burnetii DNA in clinical specimens by targeting a sequence of the shikimate dehydrogenase gene ( aroE ) unique to Coxiella burnetii . (mayomedicallaboratories.com)
  • Isolation of C. burnetii from a clinical specimen by culture. (cdc.gov)
  • The pathologic findings and distribution of C burnetii in the damaged valve tissues explain the clinical findings of valve failure and occasional embolic episodes, as well as the frequent ability to isolate C burnetii from the peripheral blood of infected patients. (elsevier.com)
  • This study suggests that preventive measures should be practiced in ruminant farms in Jordan to avoid C . burnetii infection. (ajtmh.org)
  • Prevalence data of C. burnetii infection in different ruminant species are important to support risk assessments or decisions on preventive measures regarding public and animal health. (biomedcentral.com)
  • To understand the role of major histocompatibility complex class I (MHC-I) and MHC-II in vaccine-mediated protection against Coxiella burnetii , we evaluated the protective efficacy of a formalin-inactivated C. burnetii Nine Mile phase I vaccine (PIV) in β 2 -microglobulin-deficient (B2m KO. (asm.org)
  • Levels of the resulting 23S rRNA fragments that flank the IVS, F1 (~1.2 kb) and F2 (~1.7 kb), were quantified over C. burnetii 's logarithmic growth phase (1-5 d). (frontiersin.org)
  • The localization and role of wild-type and mutated Rab5 and Rab7, markers of early and late endosomes, respectively, were also examined to determine the role of these small GTPases in the trafficking of C. burnetii phase II. (asm.org)
  • Lysosomal membrane markers and enzymes, as well as molecules internalized by fluid phase endocytosis, are easily found in vacuoles containing C. burnetii ( 23 ), and. (asm.org)
  • Transition of LCV back to SCV occurs coincident with Coxiella entry into stationary growth phase, with nearly homogeneous SCV present upon extended incubation (2 to 4 weeks) of infected cell cultures. (grantome.com)
  • To establish this unique niche, C. burnetii requires the Dot/Icm type IV secretion system (T4SS) to translocate a cohort of effector proteins into. (asm.org)
  • Also, more effort should be invested in determining time-dependent emission rates of Coxiella burnetii and defining a protocol for systematic and active surveillance (including air sampling) during future outbreaks of Coxiella burnetii or other zoonoses. (uu.nl)
  • Variation is mainly achieved through SNPs, many of which are non-synonymous, thereby confirming that evolution of C. burnetii is based on modification of existing genes. (biomedcentral.com)
  • Thus, it is not possible to distinguish between C. burnetii and CLB based on assays targeting these genes. (edu.au)
  • Mature, spacious PV containing multiple nonreplicating C. burnetii were clearly visible in infected Vero cells treated with the cell wall inhibitor carbenicillin. (nih.gov)
  • Vero cells infected by Coxiella burnetii isolated from an Amblyomma parvum tick. (cdc.gov)
  • To characterize T4BSS subcellular localization in C. burnetii, we analyzed C. burnetii-infected Vero cells by indirect immunofluorescent antibody (IFA) and immunoelectron microscopy (IEM). (nebraska.edu)