Counterimmunoelectrophoresis: Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.Immunoelectrophoresis: A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.Precipitins: Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.Latex Fixation Tests: Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)Agglutination Tests: Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)Meningitis: Inflammation of the coverings of the brain and/or spinal cord, which consist of the PIA MATER; ARACHNOID; and DURA MATER. Infections (viral, bacterial, and fungal) are the most common causes of this condition, but subarachnoid hemorrhage (HEMORRHAGES, SUBARACHNOID), chemical irritation (chemical MENINGITIS), granulomatous conditions, neoplastic conditions (CARCINOMATOUS MENINGITIS), and other inflammatory conditions may produce this syndrome. (From Joynt, Clinical Neurology, 1994, Ch24, p6)Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Meningitis, Haemophilus: Infections of the nervous system caused by bacteria of the genus HAEMOPHILUS, and marked by prominent inflammation of the MENINGES. HAEMOPHILUS INFLUENZAE TYPE B is the most common causative organism. The condition primarily affects children under 6 years of age but may occur in adults.Immunodiffusion: Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.Viruses, Unclassified: Viruses whose taxonomic relationships have not been established.Antibodies, Fungal: Immunoglobulins produced in a response to FUNGAL ANTIGENS.Streptococcus pneumoniae: A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals.Haemophilus influenzae: A species of HAEMOPHILUS found on the mucous membranes of humans and a variety of animals. The species is further divided into biotypes I through VIII.Cinchona Alkaloids: Alkaloids extracted from various species of Cinchona.Hemagglutination Tests: Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)Aleutian Mink Disease: A slow progressive disease of mink caused by the ALEUTIAN MINK DISEASE VIRUS. It is characterized by poor reproduction, weight loss, autoimmunity, hypergammaglobulinemia, increased susceptibility to bacterial infections, and death from renal failure. The disease occurs in all color types, but mink which are homozygous recessive for the Aleutian gene for light coat color are particularly susceptible.Evaluation Studies as Topic: Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.Aleutian Mink Disease Virus: A species of PARVOVIRUS that causes a disease in mink, mainly those homozygous for the recessive Aleutian gene which determines a desirable coat color.Streptococcus agalactiae: A bacterium which causes mastitis in cattle and occasionally in man.Immunoelectrophoresis, Two-Dimensional: Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.Cross Reactions: Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.Hepatitis B Antigens: Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Pneumonia, Pneumococcal: A febrile disease caused by STREPTOCOCCUS PNEUMONIAE.Neisseria meningitidis: A species of gram-negative, aerobic BACTERIA. It is a commensal and pathogen only of humans, and can be carried asymptomatically in the NASOPHARYNX. When found in cerebrospinal fluid it is the causative agent of cerebrospinal meningitis (MENINGITIS, MENINGOCOCCAL). It is also found in venereal discharges and blood. There are at least 13 serogroups based on antigenic differences in the capsular polysaccharides; the ones causing most meningitis infections being A, B, C, Y, and W-135. Each serogroup can be further classified by serotype, serosubtype, and immunotype.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Teichoic Acids: Bacterial polysaccharides that are rich in phosphodiester linkages. They are the major components of the cell walls and membranes of many bacteria.Complement Fixation Tests: Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.False Positive Reactions: Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)Typhoid Fever: An acute systemic febrile infection caused by SALMONELLA TYPHI, a serotype of SALMONELLA ENTERICA.Salmonella typhi: A serotype of SALMONELLA ENTERICA which is the etiologic agent of TYPHOID FEVER.Adenoviruses, Human: Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.Copyright: It is a form of protection provided by law. In the United States this protection is granted to authors of original works of authorship, including literary, dramatic, musical, artistic, and certain other intellectual works. This protection is available to both published and unpublished works. (from Circular of the United States Copyright Office, 6/30/2008)Pathology, Clinical: A subspecialty of pathology applied to the solution of clinical problems, especially the use of laboratory methods in clinical diagnosis. (Dorland, 28th ed.)Legionella pneumophila: A species of gram-negative, aerobic bacteria that is the causative agent of LEGIONNAIRES' DISEASE. It has been isolated from numerous environmental sites as well as from human lung tissue, respiratory secretions, and blood.Legionella: Gram-negative aerobic rods, isolated from surface water or thermally polluted lakes or streams. Member are pathogenic for man. Legionella pneumophila is the causative agent for LEGIONNAIRES' DISEASE.Tropical Medicine: The branch of medicine concerned with diseases, mainly of parasitic origin, common in tropical and subtropical regions.Echinococcosis: An infection caused by the infestation of the larval form of tapeworms of the genus Echinococcus. The liver, lungs, and kidney are the most common areas of infestation.Electrophoresis, Cellulose Acetate: Electrophoresis in which cellulose acetate is the diffusion medium.Aspergillosis, Allergic Bronchopulmonary: Hypersensitivity reaction (ALLERGIC REACTION) to fungus ASPERGILLUS in an individual with long-standing BRONCHIAL ASTHMA. It is characterized by pulmonary infiltrates, EOSINOPHILIA, elevated serum IMMUNOGLOBULIN E, and skin reactivity to Aspergillus antigen.Aspergillus fumigatus: A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.Aspergillosis: Infections with fungi of the genus ASPERGILLUS.Pulmonary Aspergillosis: Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).Aspergillus: A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.Nerve Endings: Branch-like terminations of NERVE FIBERS, sensory or motor NEURONS. Endings of sensory neurons are the beginnings of afferent pathway to the CENTRAL NERVOUS SYSTEM. Endings of motor neurons are the terminals of axons at the muscle cells. Nerve endings which release neurotransmitters are called PRESYNAPTIC TERMINALS.Word Association Tests: Lists of words to which individuals are asked to respond ascertaining the conceptual meaning held by the individual.Vocabulary: The sum or the stock of words used by a language, a group, or an individual. (From Webster, 3d ed)Muscle Spindles: Skeletal muscle structures that function as the MECHANORECEPTORS responsible for the stretch or myotactic reflex (REFLEX, STRETCH). They are composed of a bundle of encapsulated SKELETAL MUSCLE FIBERS, i.e., the intrafusal fibers (nuclear bag 1 fibers, nuclear bag 2 fibers, and nuclear chain fibers) innervated by SENSORY NEURONS.Semantics: The relationships between symbols and their meanings.Echinococcosis, Hepatic: Liver disease caused by infections with parasitic tapeworms of the genus ECHINOCOCCUS, such as Echinococcus granulosus or Echinococcus multilocularis. Ingested Echinococcus ova burrow into the intestinal mucosa. The larval migration to the liver via the PORTAL VEIN leads to watery vesicles (HYDATID CYST).Echinococcosis, Pulmonary: Helminth infection of the lung caused by Echinococcus granulosus or Echinococcus multilocularis.Mebendazole: A benzimidazole that acts by interfering with CARBOHYDRATE METABOLISM and inhibiting polymerization of MICROTUBULES.Echinococcus granulosus: A species of hydatid tapeworm (class CESTODA) in the family Taeniidae, whose adult form infects the DIGESTIVE TRACT of DOGS, other canines, and CATS. The larval form infects SHEEP; PIGS; HORSES; and may infect humans, where it migrates to various organs and forms permanent HYDATID CYSTS.

Immunodiagnosis of histoplasmosis in a compromised host. (1/267)

Three serological tests for the diagnosis of histoplasmosis were compared for sensitivity and specificity in serum from blood bank donors, patients with histoplasmosis, and infected or noninfected immunosuppressed patients. The histoplasmin latex agglutination test was positive in 9% of the normal patients, 33% of the histoplasmosis patients, and 61% of the noninfected immunosuppressed patients. Since the test is prone to many false-positive results in patients with inflammatory diseases or non-Histoplasma infections, it has limited potential as a screening test among compromised patients. Immunodiffusion and counterimmunoelectrophoresis using a mycelial antigen were found to be more sensitive than either test using a combined yeast and mycelial antigen or a pure yeast phase antigen. Counterimmunoelectrophoresis at pH 7.2 proved to be the test of choice for serodiagnosis of histoplasmosis, resolving 85% of the immunocompetent infected patients and 100% of the infected immunosuppressed patients. Results indicated that counterimmunoelectrophoresis in conjunction with immunodiffusion could be used as a screening protocol to determine infection in incoming patients in a cancer hospital.  (+info)

Counterimmunoelectrophoretic detection of a high incidence of precipitin reactions in normal human sera against staphylococcal teichoic acids and protein A. (2/267)

The use of counterimmunoelectrophoresis (CIE) for detection of serum antibodies to staphylococcal teichoic acids was evaluated against teichoic acids prepared by sonic treatment or lysostaphin extraction of Staphylococcus aureus (Lafferty strain). Of 54 patient sera from suspected cases of staphylococcal endocarditis, osteomyelitis, or septicemia, 33 (61.1%) were positive by CIE analysis; however, 128 of 291 sera (44.0%) from normal adult donors were also positive. Selected CIE-positive sera from patient and control groups were titered by Ouchterlony gel diffusion. In the control group of normal sera, 65% were also positive by gel diffusion, but only 15% had titers of >/=1:2. Of the patient sera, 44.4% had gel diffusion titers of >/=1:2. In addition to the specific teichoic acid band, a second precipitation band could be demonstrated with both patient or normal sera by CIE or gel diffusion. This second precipitin band was shown to involve interactions of test sera with staphylococcal protein A present in the teichoic acid extracts. The protein A precipitins were detected at high concentrations of the antigen extracts, whereas the anti-teichoic acid precipitins were optimally detected at lower antigen concentrations. The formation of protein A precipitin bands did not correlate with the presence of anti-teichoic acid antibodies, as most sera tested were positive for protein A regardless of anti-teichoic acid activity. This study suggests that a high incidence of normal people have levels of antibodies to teichoic acids which are detectable by the highly sensitive, but nonspecific, technique of CIE.  (+info)

Detection of pneumolysin in sputum. (3/267)

Western blot detection of the species-specific pneumococcal product, pneumolysin (SPN), was shown to be almost as sensitive as PCR for the non-cultural detection of pneumococci in 27 Streptococcus pneumoniae culture-positive sputa from patients stated to have chest infections. Both techniques were considerably more sensitive than counter-current immuno-electrophoresis for pneumococcal capsular polysaccharide antigens (CPS-CIE) on the same specimens. Sensitivities for PCR, SPN-immunoblotting and CPS-CIE were 100%, 85% and 67%, respectively. In 11 S. pneumoniae culture-negative sputa taken from patients receiving antibiotics, but with proven recent pneumococcal infection, PCR and SPN-blot were positive in six (in two of which CPS-CIE was also positive), PCR alone was positive in one and SPN-blot alone was positive in one. In 11 S. pneumoniae culture-negative samples from patients not receiving antibiotics, all three tests were negative in eight, PCR was positive in three (in one of which CPS-CIE was also positive), but SPN-blot was negative in all 11. In 16 S. pneumoniae culture-negative samples from patients receiving antibiotics and with no known recent pneumococcal infections, one or more non-cultural test was positive in 11. Although further evaluation is required to assess the significance of pneumolysin detection in relation to carriage and infection and to devise a more suitable test format, these preliminary studies suggest that pneumolysin detection is a promising new approach to the non-cultural diagnosis of pneumococcal chest infection.  (+info)

Pneumococcal antigen in pneumonia. A post-mortem study with the histological and bacteriological findings. (4/267)

Pneumococcal capsular antigens can be detected in lung tissue by counter-current immunoelectrophoresis even when, following antibiotics, post-mortem bacterilogy suggests that Escherichia coli has replaced pneumococci. The results suggest that antipneumococcal therapy would benefit at least 55% of patients critically ill with lung infection and that the potentially toxic drugs directed at coliform bacteria may be unnecessary.  (+info)

Evaluation of tests for antibody response in the follow-up of patients with acute and chronic forms of paracoccidioidomycosis. (5/267)

Several serological tests have been used successfully in the diagnosis of paracoccidioidomycosis (PCM). In contrast, data about the use of these tests in the follow-up of PCM patients have been heterogeneous. In this study, serum samples from 43 PCM patients with different clinical forms were analysed by counter-immuno-electrophoresis (CIE), complement fixation (CF) and ELISA before treatment. With CIE and ELISA, the chronic unifocal form showed significantly lower antibody levels compared with chronic multifocal and acute forms. Acute form patients had significantly higher titres than patients with multifocal disease by CIE but not by ELISA. No significant differences were observed with CF. Twenty-seven of these patients were followed-up for 2 years and showed a decline in antibody levels by all three tests, paralleling clinical improvement. However, only patients with unifocal disease cleared their antibodies after 1 year of treatment as analysed by CF and ELISA and after 2 years by CIE, suggesting that these patients may need shorter courses of therapy. Patients with the other clinical form of the disease needed > or =2 years of therapy to clear their antibodies. Sera from a further five patients who presented with a relapse were analysed. At the time of relapse all showed increases in antibody levels by CIE and ELISA, but only three showed increases by CF tests. Therefore, CIE and ELISA demonstrated a better clinical correlation than CF, probably reflecting the fungal burden of PCM patients more accurately.  (+info)

Alkyl hydroperoxide reductases C and D are major antigens constitutively expressed by Mycobacterium avium subsp. paratuberculosis. (6/267)

Antigens characteristic for Mycobacterium avium subspecies paratuberculosis were identified by crossed immunoelectrophoresis (CIE) and by absorbing out cross-reactive antigens by using a polyclonal and polyvalent Mycobacterium avium subspecies avium antiserum. Two antigens were present in M. avium subsp. paratuberculosis and not detected in Mycobacterium avium subsp. avium. They were identified as antigens 17 and 20 in a CIE reference system for M. avium subsp. paratuberculosis antigens. Purified antigen 20 was identified as alkyl hydroperoxide reductase C (AhpC) while the N-terminal part of purified antigen 17 showed 80% homology with alkyl hydroperoxide reductase D (AhpD) of Mycobacterium tuberculosis. AhpC had a nonreduced mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis corresponding to a molecular mass of 45 kDa and is probably a homodimer linked with disulfide bridges in its native form. AhpD had a mobility corresponding to 19 kDa. Monospecific rabbit antiserum against AhpC and AhpD reacted with 9 strains of M. avium subsp. paratuberculosis but not with 20 other mycobacterial strains except for a Mycobacterium gordonae strain, against which a weak cross-reactive band was produced. Goats experimentally infected with M. avium subsp. paratuberculosis had strong gamma interferon (IFN-gamma) responses toward both AhpC and AhpD, and they also had antibodies against AhpC. The ability of AhpC and AhpD to induce IFN-gamma production shows that these proteins potentially could be used in future vaccines or in diagnostic assays. These results further show that AhpC and AhpD are immunologically important proteins which are constitutively and highly expressed in M. avium subsp. paratuberculosis without the bacteria being submitted to oxidative stress and that the specificities of antigens can be a matter of different levels of protein expression in various species as well as distinct structural differences.  (+info)

Immune response in cattle vaccinated against rabies. (7/267)

In order to determine the best type of rabies vaccine to use as a booster, 78 serological samples from singly vaccinated cattle were analyzed by counterimmunoelectrophoresis technique. The animals were divided into several groups, received the first vaccine dose with modified live virus vaccine (ERA strain) and were revaccinated with inactivated virus or modified live virus vaccines. Boosters were given at 2, 4, 8, 12 and 16 weeks following first vaccination. Results showed high titres in the cases of booster with inactivated vaccine. In all cases, however, detectable antibody titres declined quickly.  (+info)

Canine experimental infection: intradermal inoculation of Leishmania infantum promastigotes. (8/267)

Five mixed breed dogs were inoculated intradermally (ID) with cultured virulent stationary phase promastigotes of Leishmania infantum Nicole, 1908 stocks recently isolated. Parasite transformations in the skin of ID infected dogs were monitored from the moment of inoculation and for 48 h, by skin biopsies. Anti-Leishmania antibody levels were measured by indirect immunofluorescence assay, counterimmunoelectrophoresis and direct agglutination test, and clinical conditions were examined. Thirty minutes after ID inoculation the first amastigotes were visualised and 3 to 4 h after inoculation the promastigotes were phagocytized by neutrophils and by a few macrophages. These cells parasitised by amastigotes progressively disappeared from the skin and 24 h after inoculation parasites were no longer observed. Local granulomes were not observed, however, serological conversion for antibodies anti-Leishmania was achieved in all dogs. Direct agglutination test was the only technique positive in all inoculated dogs. Amastigotes were found in the popliteal lymph node in one dog three months after inoculation. This work demonstrates that, with this inoculum, the promastigotes were transformed into amastigotes and were up taken by neutrophils and macrophages. The surviving parasites may have been disseminated in the canine organism, eliciting a humoral response in all cases.  (+info)

*Counterimmunoelectrophoresis

... is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is ... http://medical-dictionary.thefreedictionary.com/counter+immunoelectrophoresis Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. ( ... ISBN 0-8385-8529-9. Counterimmunoelectrophoresis at the US National Library of Medicine Medical Subject Headings (MeSH) http:// ...

*Allergic bronchopulmonary aspergillosis

Both are more sensitive than conventional counterimmunoelectrophoresis. IgG may not be entirely specific for ABPA, as high ...

*Pseudallescheria boydii

In serum, Scedosporium infections can be detected by counterimmunoelectrophoresis. Molecular diagnostics appear to be promising ...

*George Siber bibliography

Siber, GR; Skapriwsky, P (Apr 1978). "Counterimmunoelectrophoresis for detection of microbial antigens: increased sensitivity ... influenzae type b infections by latex particles agglutination and counterimmunoelectrophoresis. Presented at the Society for ... "Rapid diagnosis of Hemophilus influenzae type b infections by latex particle agglutination and counterimmunoelectrophoresis". ...

*Aleutian disease

When evidence of ADV shows in a ferret, it is strongly recommended that a CEP (counterimmunoelectrophoresis) blood test or an ...

*List of MeSH codes (E01)

... counterimmunoelectrophoresis MeSH E01.450.495.735.645.350.350.350 --- immunoelectrophoresis, two-dimensional MeSH E01.450. ...
Abstract A modified counterimmunoelectrophoresis and a conventional indirect hemagglutination test were compared for routine diagnosis of human hydatid disease in an endemic area in the Middle East. Counterimmunoelectrophoresis was performed on a cellulose acetate membrane with dilutions of a commercially available antigen which interacts with sera of patients with confirmed hydatid disease to produce the arc 5 precipitin line. The test was performed with unconcentrated human sera and the lines stained in an aqueous solution of Ponceau red. Sensitivity (95.5% vs. 93.2%) and specificity (99.2% vs. 89.9%) were higher with counterimmunoelectrophoresis than with indirect hemagglutination. Cross-reactivity with sera of patients with other parasitic infections was noted with indirect hemagglutination but not with counterimmunoelectrophoresis. There was no cross-reactivity with sera of patients with autoimmune disorders by either test.
The complex antigenic properties of adenovirus-soluble complete and incomplete hemagglutinins were utilized to study the specificity of adenovirus anti-hemagglutinin sera in immunodiffusion and counterimmunoelectrophoresis tests. Pentons, penton dimers, fiber dimers, and/or dodecons of adenovirus types 2, 3, 5, 7a, 8, and 11 were extensively purified by ion-exchange and exclusion chromatographies. Components were determined to be purified proteins by immunologic, electron microscopic, and polyacrylamide gel electrophoretic criteria. Specific antigenic components were identified by immunoelectrophoresis and by various biologic characteristics. Rabbit antisera prepared against these hemagglutinating components were type-specific in standard serologic, Ouchterlony immunodiffusion, immunoelectrophoretic, and counterimmunoelectrophoretic tests. Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for diagnostic purposes.. ...
The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella typhi were compared with those of the Widal test in the serological diagnosis of typhoid fever. A veronal buffer extract yielded precipitation lines against 50 (96%) out of 52 sera collected from patients with typhoid but against none out of 62 sera obtained from control subjects who did not have typhoid. Less satisfactory results were obtained by counterimmunoelectrophoresis when two other preparations, a protein extract (Barber antigen) and an ultrasonic lysate, were used as the antigens. By Widal test the rate of detection in patients sera was 73% and the false-positive rate for control sera 16%. It is concluded that in an area where typhoid is endemic counterimmunoelectrophoresis using veronal buffer extract as the antigen is superior to the Widal test for serological diagnosis of typhoid fever. An additional advantage of counterimmunoelectrophoresis is that results ...
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To ascertain the heterogeneity of anti-prothrombin antibodies (aPT), we compared three in-house aPT ELISAs: A) medium binding plates, phosphatidylserine, prothrombin, Tris-buffered saline, calcium; B) high binding plates, prothrombin, Tris-buffered saline; and C) high binding plates, prothrombin, PBS. One serum, exhibiting high positive aPT in all three ELISAs, was selected as the calibrator. Sera from 47 patients (41 with SLE, 4 with pAPS, 2 with arterial thromboses) were tested for IgG and IgM aPT.. The results showed six different patterns: 1) similar results were obtained with A, B and C (similar analytical sensitivity); 2) similar results were obtained with A and B while C showed lower analitycal sensitivity; 3) B and C seemed analytically less sensitive than A; 4) A and B were analytically less sensitive than C; 5) A showed very low analytical sensitivity; 6) A and C showed lower analytical sensitivity than B.. The analysis of all the presented patterns showed noncomparable results of the ...
Anti-Prothrombin antibody conjugated to Biotin validated for WB, IP, RIA, cELISA and tested in Human. Immunogen corresponding to full length native protein…
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Haemophilus influenzae type B Antigen Detection, LA; Neisseria meningitidis Antigen Detection (Groups A/Y and C/W 135), LA; Neisseria meningitidis Detection (Group B/E. coli K1), LA; Streptococcus, Group B, Antigen Detection, LA; Streptococcus pneumoniae Antigen Detection, LA ...
Approximate amount. All fees are tentative and subject to change. The application fee is nonrefundable. Tuition fees generally increase starting in August. Click here to download a PDF of the CIEP Dates & Fees. ...
Preparing for the CCIE Routing and Switching lab exam typically involves massive amounts of studying. But if you already possess Ciscos CCNP Routing and Switching certification, you already know much of what youll need to succeed on CCIEs labs. This book will help you bridge the gaps in your knowledge quickly and efficiently, and make the most of everything you already know.
Please note: CIE helpdesk is intended to give assistance regarding questions related to CIE publications or the technical work in CIE Technical Committees.. It is not intended to give advice or even provide technical solutions to questions such as what CIE publication to use or queries relating to the interpretation of CIE publications. We hope for your understanding that we will not reply to inquiries of that kind. Please check the CIE website regularly to stay informed about conferences, symposia or trainings for further information and training.. ...
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CIE has formal arrangements with several international and regional organizations, these arrangments are supported by liaison at the administrative level of these orgainzations. CIE also has working liaisons with both these and other orgainzaitons at the technical level, these working liaisons can be found within the Division pages of this website.. ...
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Intróny mohli byť pôvodne genómoví paraziti typu vírusov a transpozónov, ktoré bunke neprinášajú úžitok, iba sa množia tým, že sa nakopírujú a vložia sa na ďalšie miesto v genóme hostiteľa. V populácii sa šíria vertikálne, t.j. z rodiča na potomka. Niektoré intróny sú schopné autosplicingu, vďaka čomu sa dokážu vystrihnúť z mRNA bez cudzej pomoci. Aby intróny nezabíjali bunky, musia sa vedieť v priebehu translácie inaktivovať, na čo slúži práve splicing. ...
Powstanie rezolucja w sprawie zmian, jakie posłowie planują w Ustawie o obronie zwierząt w kontekście uboju rytualnego w Polsce? Hodowc
XXVI/4 - Iné poruchy hybnosti centrálneho pôvodu, poruchy hybnosti po zápaloch mozgu a miechy, degeneratívne a heredofamiliárne ochorenia ovplyvniteľné liečebnou rehabilitáciou, poruchy hybnosti po cievnych a mozgových príhodách, poruchy hybnosti po úrazoch mozgu, poruchy hybnosti po operáciách benígnych nádorov centrálneho nervového sytému do 12 mesicov od operácie alebo vzniku ochorenia ...
Viac informácií o benzol-1-carbonsaeure-4-sulfonsaeuredipropylamid. Umožňujeme pokrok vo vede tým, že ponúkame výber produktov, servisné služby, vedecké inovácie, neustále zlepšovanie a zameriavanie sa na zákazníka.
Semperit Semp Van-Life 195/70 R15 C 104/102 S Letné lacne a SKLADOM. Nakúpte pneu Semperit Semp Van-Life výhodne na E-pneumatiky.sk. Všetky informácie, parametre i energetické štítky.
Uniwersytet Przyrodniczy we Wrocławiu. PI - Monitoring losów absolwentów w kontekście dostosowania oferty edukacyjnej do potrzeb gospodarki opartej na wiedzy i wymogów rynku pracy
Intímny sprchový gél na hygienu žien vo fertilnom veku, vhodný po športovaní, počas menštruácie, pri gynekologických problémoch a zápachu intímnych partií
The SET-RPLA, from Denka Seiken Co. Ltd., Tokio, a commercial reversed passive latex agglutination test kit, has been recommended to establish the enterotoxicity capacity of some staphylococcal strains, implicated in food poisoning outbreaks that produce low levels of enterotoxins (SE). Despite the RPLA specificity, the occurrence of nonspecific reactions when testing low- SE-producing is common. In order to control these nonspecific reactions the addition of purified normal rabbit IgG purified was applied on approximately 350 staphylococcal isolates from human milk and anatomic sites of healthy dental student carriers. The results indicated that addition of 5% (v/v) of purified normal rabbit IgG (0.74 mg/mL) to the culture supernatant fluid is a simple and reliable tool for the controlling of nonspecific reactions in the RPLA assay ...
In a series of 163 ears with chronic, mucoid effusion, free or immune complex bound pneumococcal capsular polysaccharide antigens were demonstrated with Omniserum and counterimmunoelectrophoresis (CIEP) in 37 ears (23%). In crushed adenoid tissue thi
Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV. The results were compared to those obtained for 75 non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P. brasiliensis antibodies were detected in 65% (DID), 79% (CIEP) and 95% (ELISA) of the patients with HIV/AIDS, significantly lower rates than those detected in cases of endemic paracoccidioidomycosis, which were 89%, 99% and 100%, respectively. The reactive sera of HIV-infected patients also showed lower anti-P. brasiliensis antibody ...
TY - JOUR. T1 - La sierologia del Lupus eritematoso. AU - Parodi, A.. AU - Cozzani, E.. AU - Drosera, M.. PY - 2004. Y1 - 2004. N2 - Lupus erythematosus (LE), expecially its systemic form (SLE), is serologically characterized by the presence of autoantibodies directed to nuclear, cytoplasmic and cellular membrane antigens. Some of them are specific of the disease, others characterize only particular substes. Antinuclear antibodies (ANA) can be detected by various assays: indirect immunofluorescence (IIF), immunodiffusion (ID), counterimmunoelectrophoresis (CIE), enzyme linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and immunoblotting (IB). IIF and ELISA are the most used. ELISA is very sensitive, IIF is sensitive, easy to perform and cheap. In IIF monolayers of cultured cells particularly Hep-2 are the best substrate to use. Antibodies to ds-DNA are high specific of SLE and can be used to monitor the clinical course of the disease. Anti-nucleosome antibodies are now considered the ...
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Definition : Serology reagents intended to detect antibodies to teichoic acid, a polymer found in the cell wall and cell membrane of some bacteria. High levels of teichoic acid antibodies in serum indicate the possible presence of bacteria, especially occult Staphylococcus aureus infection.. Entry Terms : "Teichoate Antibody Detection/Identification Reagents" , "Teichoic Acid Antibody Detection/Identification Reagents" , "Reagents, Serology, Bacteria, Teichoic Acid, Antibody". UMDC code : 17396 ...
Prednášky pre 6.ročník „Zubné lekárstvo" zimný semester ak. Rok 2016/2017 Prednášky: 1. - 7., 9. - 13. týždeň „Maxilofaciálna chirurgia", poslucháreň Heydukova (utorok 16.30 - 18.20 hod.) 1. Dentoalveolárna chirurgia - opakovanie (anatómia dutiny ústnej, lokálna anestézia, indikácie, komplikácie lokálne, celkové, Extrakcie zubov, indikácie, technika, komplikácie lokálne, endodonticko- chirurgické ošetrenie devitálnych zubov, indikácie, technika, rtg) 2. Dentoalveolárna chirurgia - opakovanie (tretie moláre, vyšetrenie, indikácie extrakcie, asymptomatický tretí molár, technika extrakcie, chirurgická extrakcia, komplikácie, pooperačná starostlivosť) 3. Dentálna implantológia (princípy, základné typy dentálnych implantátov, chirurgická technika, indikácie, protetická sanácia defektov na dentálnych implantátoch) 4. Pacient na antiagregačnej a antikoagulačnej liečbe ( princípy a typy antiagregačnej a antikoagulačnej liečby, ...
Táto práca preukázala, že pravidelná fyzická aktivita strednej intenzity spôsobuje významné zmeny lipoproteínového profilu už v priebehu prvých 14 dní. Priaznivý efekt cvičenia na lipidový metabolizmus bol zdokumentovaný mnohými prácami. Tieto sa však líšili významne dlhším trvaním tréningového protokolu (2 mesiace až 1 rok) [10- 13,15]. Sledovanie zmien v takto krátkom období doposiaľ nebolo v tomto rozsahu v nami dostupnej literatúre realizované. Výrazné zmeny sme pozorovali v oblasti lipoproteínov bohatých na triacylglycerol (VLDL, IDL). Naša práca preukázala štatisticky významné zníženie koncentrácie VLDL v priebehu 14 dní. V literatúre je opísané zníženie koncentrácie VLDL a triacylglycerolu (TAG) pôsobením tréningového programu s využitím aeróbneho cvičenia strednej intenzity o trvaní 6 mesiacov [11,13,15]. K najmarkantnejšiemu zníženiu koncentrácie VLDL dochádza podľa literatúry u jedincov s ...
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Anyhow a couple of months ago my CCIE study came to an end not because I passed the lab unfortunatly but because the kids were to much of a handful for my wife to handle on her own on weekends. I do hope that not everything has fallen out of my head and that I can pick it up again sometime in the near future maybe in winter where I can trick my kids into believing it is bed time at 3pm in the afternoon :-) It will not be any time soon though because my lab is in pieces as I have decided to move house. Not only have I decided to move into a bigger more expensive house when interest rates are at their lowest levels in hundreds of years but I have also decided to move jobs and take a pay cut at the same time. Nothing like making life difficult for yourself hey? As you can see I am going stuggle with that CCIE as I am not the brightest lamp on the street ...
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CIE 10 PREMIUM線上破解APP新玩法懶人包,詳細解說醫療APP手遊最新APP消息,不能錯過全世界Android App Store、iOS App Store、Windows App Store搶先資訊ICD 10 Indonesia-English app,idc 10熱門APP載點排行榜,idc 10 pinLa CIE10 es el acrónimo de la Clasificación internacional de enfermedades, décima versión correspondiente a la versión en español de la (en inglés) IC
This report is a revision and update of CIE 150:2003 Guide on the Limitation of the Effects of Obtrusive Light from Outdoor Lighting Installations. The purpose of this Guide is to help formulate guidelines for assessing the... more ...
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Kyselina acetylsalicylová, kombinácie s výnimkou psycholeptík, Granulát na perorálnu suspenziu, ATC N02BA51, SPC (Súhrn údajov o prípravku) Terapeutické indikácie: Symptomatická liečba upchatého nosa/nosných dutín (rinosinusitídy) s bolesťou a horúčkou, ktoré sa spájajú s nachladnutím, a/alebo príznakmi podobnými chrípke. ASPIRIN COMPLEX je indikovaný dospelým a dospievajúcim vo veku od 16 rokov.
A designação da OTAN para cada míssil está mostrada entre parênteses, antes do nome próprio.. AA = Ar-Ar, do inglês Air-to-Air;. SA = Superfície-Ar, do inglês Surface-to-Air(p.ex., SAMs);. SS = Superfície-Superfície, do inglês Surface-to-Surface;. SA-N = Superfície-Ar, Naval, do inglês Surface-to-Air, Naval;. SS-N = Superfície-Superfície, Naval do inglês Surface-to-Surface, Naval (p.ex., mísseis lançados de navios ou submarinos).. ...
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NR. 67 p. 1 p. 4 p. 8 p. 14 Bogusław Jasiński To, co ukryte What is hidden The art of kamp offers the carnival for senses. It seems to think in the context of popular art. It is an important artistic trend
O projeto de novos materiais para aplicações tecnológicas em biomateriais e bioengenharia é altamente dependente de como as células aderem à superfície de um material. A adesão e crescimento em biomateriais...
KOMISJA EUROPEJSKA BRUKSELA, DNIA 23/09/2014 R. BUDŻET OGÓLNY 2014 SEKCJA III KOMISJA TYTUŁY 01, 05, 07, 08, 09, 12, 13, 16, 17, 18, 19, 20, 21, 23, 24, 26, 29, 32, 33, Wprowadzenie PRZESUNIĘCIE
Produkty držiteľa rozhodnutia Berlin-Chemie Menarini (DEU) registrované v SR. Informácie o výdaji, doplatkoch, spôsobe užívania. Rozsiahle možnosti vyhľadávania. Denne aktualizované.
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Abstract Echography and/or chest radiograph in association with six serologic (immunologic) methods were tested for their ability to diagnose human hydatidosis. The immunologic techniques used were latex agglutination, counter immunoelectrophoresis, enzyme-linked immunosorbent assay (ELISA) with whole hydatid fluid and with antigen 5, a thermobabile lipoprotein that elicits the arc 5 precipitin line in immunoelectrophoresis. In this report, the results of examinations of 273 patients are presented. Of these, 243 cases were surgically proven to have hydatidosis, whereas 30 of the 273 that were strongly suspected of having hydatid cysts by radiology were shown to have other pathologies. The ELISA was more sensitive than the other methods, with 204 of 243 sera shown to be positive. The remaining 39 sera that gave false-negative results were tested using two recently developed methods, enzyme-linked immunoelectrodiffusion assay (ELIEDA) and immunoblotting (electrophoretic migration of hydatid fluid antigens
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A process for upgrading a liquid petroleum or chemical stream wherein said feedstream flows countercurrent to the flow of a treat gas, such as a hydrogen-containing gas, in at least one reaction zone. The feedstream is treated so that it is substantially free of particulate matter and foulant precursors.
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Tento dokument informuje používateľov distribúcie Debian o hlavných zmenách vo verzii 9 (kódové označenie „stretch"). Poznámky k vydaniu poskytujú informácie o tom ako bezpečne aktualizovať systém z predošlého vydania 8 (kódové označenie jessie) na aktuálne vydanie a informujú používateľov o známych potenciálnych problémoch, s ktorými by sa mohli stretnúť počas aktualizácie. Najnovšia verzia tohto dokumentu je vždy dostupná na https://www.debian.org/releases/stretch/releasenotes. Ak si nie ste istý či čítate aktuálnu verziu, skontrolujte dátum na prvej stránke a uistite sa, že čítate aktuálnu verziu. ...
1) NCEA: at least 14 credits NCEA Level 3 Chemistry, or (2) CIE: at least D grade in CIE AL Chemistry or A grade in CIE ASL Chemistry, or (3) IB: at least Grade 4 in IB HL Chemistry or Grade 6 in IB SL Chemistry, or (4) CHEM114, or at least B Grade in BRDG022.. ...
BS 3900:Introduction, BS 3900:Part D1, BS 3900:Part D5, BS 3900:Part D9, BS 3900:Part D10, CIE 15, CIE 15:Supplement No. 2, CIE 38 ...
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Witajcie po bardzo długiej przerwie. Już do Was wracam! Mam nadzieję, że podczas mojej nieobecności sumiennie przerobiliście wszystkie lekcje ;) Oj wiem, wiem - wakacje :) Pozostając więc w klimatach wakacyjnych, przez najbliższy czas będę przygotowywała lekcje plenerowo-domowe. Lekcja będzie rozpoczynała się na dworze, a kończyła w domu - oczywiście dziełem plastycznym. Zatem zaczynamy lekcje…
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Náš sortiment bazénov zahŕňa vírivé vane, infrasauny a doplnky k bazénom ako napríklad rebríky, vysávače, filtrácie, čerpadlá, a ďalšie.
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Anti-Jo-1 antibody: a marker for myositis with interstitial lung disease. Bernstein, R.M.; Morgan, S.H.; Chapman, J.; Bunn, C.C.; Mathews, M.B.; Turner-Warick, M.; Hughes, G.R.V. // British Medical Journal (Clinical Research Edition);7/21/1984, Vol. 289 Issue 6438, p151 Discusses the function of anti-jo-1 antibody in patients with myositis and cryptogenic fibrosing alveolitis in Great Britain. Definition of myositis; Identification of the antibody by counterimmunoelectrophoresis; Usage of cytotoxic drugs. ...
and chronic bronchitis. Some of them [29] were collected before administration of treatment for at least 7 days before admission the other [31] were collected after initiation of treatment. Each group included different ages and sexes and examined thoroughly clinically and radiologically. Sputa of each group were examined by three method for detection of Streptococcus pneumoniae which is the cause of the most fatal chest diseases. These methods were Gram film, culture and latex agglutination test for pneumococcal antigen detection. In the first group the highest positive results were obtained by Gram film 69% while culture and antigen detection were apparently equal% 55In the second group the highest positive results were obtained by antigen detection latex agglutination test 80.6%, Gram film was positive in 51.6% and culture was positive in only 32.2%. As regard susceptibility of pneumococci to different penicillin generations we found that it is still the drug of choice for the treatment of ...
A total of 15 stone artifacts that included stones from rock-lined pits, plane scrapers and agave knives were submitted for protein residue analysis from several Archaic and Formative period sites located within the Fort Bliss Military Reservation. Previous to this study, burned limestone rocks were submitted to PaleoResearch for organic (FTIR) analysis from these same sites, as well as from a few other sites in the general area (Logan 2010). FTIR analysis was successful in detecting plant residues on these artifacts. The objective of the current analysis is to detect plant and animal protein residues on the 15 stone artifacts using cross-over immunoelectrophoresis (CIEP). Matches with known antisera using CIEP can have greater taxonomic resolution than FTIR analysis; however, potential matches are limited to the available antiserum.
Okay so you might think what the hell has learning Perl got to do with my CCIE. Although it might be a bit of a distraction from my CCIE studies I believe that learning Perl will help my networking career. The senior engineer where I work now uses Perl to go out to all of our routers and automatically back them up. There are tools like CatTools and so on that already do this for you but he also uses it to interogate configs make changes and generally make managing the whole infrastructure a whole lot easier. So I think that Perl will really help me in my career, now the only problem is how to go about learning it. I am not really a programer at heart and I think that a book will be the best way for me to go about learning Perl. The book I am leaning towards is Learning Perl 5th Edition by OReilly so if anyone has any experience of this book or any others that would help me learn Perl, then please let me know ...
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Larbi Hamidi, Chairman of Santé Cie: "This is a new impetus for our business. Well be able to actively contribute to consolidating the French market and launching our European expansion, but also investing in innovative solutions to improve the efficiency of care pathways in the face of new challenges posed by connected healthcare and telehealth.". Jean-Bernard Lafonta, Chairman of HLD Europe, said: "Were proud of Santé Cies journey since 2015 to have become one of the leading home medical assistance players in France. Were delighted to continue our commitment alongside Santé Cies management to develop the business internationally and explore promising new segments such as dialysis at home.". Olivier Jarrousse, Managing Partner of UI Gestion, added: "Were very pleased with Santé Cies progress over the past 5 years and we wish to continue our active contribution to the Groups development.". Yann Bak, Managing Director in the Ardian Buyout team, said: " Were proud to enter Santé ...
Network/Data center engineer (3654459) - I am currently holding my CCIE certification in Data Center. working in Virtual and - Find and hire freelancers on Guru
Creating new databases of archival papers in modern technology, assigning DOI numbers, language correction of manuscripts, creating new databases of reviewers, introduction of anti-plagiarism detection and improvement of publishing process of Journal of Animal and Feed Sciences are financed in the years 2016-2017 by the Ministry of Science and Higher Education from the founds for science popularization activities, Contract No. 730/P-DUN/2016 ...
Všeobecná dermatológia pre deti a dospelých. Preventívne prehliadky - kontrola materských znamienok a prítomnosti rakoviny kože. Laserové, nechirurgické riešenia rušivých problémov s pokožkou.. Bezpečné a efektívne laserové terapie v jednom z najlepšie vybavených esteticko-medicínskych zariadení Európy. Ošetrenie ciev, depilácia, odstránenie tetovania, vyhladenie vrások a spevnenie pokožky. Pozoruhodné obnovenie a omladenie pokožky s prírodným efektom - 25-ročné skúsenosti.. Ordinácia plastickej a cievnej chirurgie. Jednodňové chirurgické operácie v exkluzívnom prostredí súkromnej kliniky. Unikátny laserové pozdvihnutie tváre a spevnenie krku, podbradku, moderná alternatíva klasickej operácie pozdvihnutia tváre ...
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Internetov str nka Rodinnej Farmy Ma ekov Mlyn bola vytvoren za elom zhroma ovania inform ci a ich spr stupnenie a v menu na rovni fariem. Sl i na odovzd vanie inform ci a poznatkov medzi po nohospod rsky zameran mi subjektami a farmami s cie om pom ha jej n v tevn kom n js praktick inform cie a u ito n kontakty v oblasti tradi n ho farm rstva zoh ad uj ce hist riu a miestne zvyky.
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In Merck & Cie v. Gnosis S.p.A., the Federal Circuit affirmed the decision of the USPTO Patent Trial and Appeal Board (PTAB) that held the challenged…
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Písomná informácia pre používateľov. Informácia o použití, čítajte pozorne!. CEFALOTIN 1,0 BIOTIKA. prášok na injekčný roztok. (cefalotinum natricum). Zloženie lieku. Liečivo: Cefalotinum natricum 1,058 g zodpovedá 1,0 g cefalotinum v 1 fľaštičke.. Pomocná látka: žiadna. 1 g Cefalotinu Biotikaobsahuje 2,39 mmol sodíka.. Farmakoterapeutická skupina. Antibiotikum. Charakteristika. Širokospektrálne polosyntetické antibiotikum 1. generácie cefalosporínov určené na parenterálnu aplikáciu. Rovnako ako ostatné betalaktámové antibiotiká blokuje syntézu bunkovej steny baktérií. Je odolný voči niektorým betalaktamázam. Cefalotín veľmi dobre účinkuje proti grampozitívnym kokom vrátane streptokokov skupín A,B,C,G a ďalším, tiež pneumokokov (s výnimkou kmeňov rezistentných voči penicilínu) a stafylokokov (vrátane kmeňov produkujúcich penicilinázu, ale s výnimkou kmeňov rezistentných voči meticilínu (oxacilínu)). Z gramnegatívnych ...
Číslo patentu: E 13665. Dátum: 15.04.2010. Autori: Gelmi Maria Luisa, Fontana Gabriele, Gassa Federico. MPK: C07C 227/32. Značky: derivátov, izoserínových, spôsob, přípravy. Text:. ...diastereoizoméry po jednoduchom kryštalizačnom kroku.0019 Podla tohto spôsobu imín 4 sa najskôr generuje in situ z aldehydu (RCHO) a amínu(RzNHg, kde PhCHZNHZ je výhodný) v acetonitrile pri teplote miestnosti a v prítomnosti molekulámych sít alebo pomocou destilácie azeotropickej zmesi acetonitril/voda. Reakčná teplota je znížená na -30 °C a pridáva sa silylový derivát 3. Potom sa pridáva katalyzátor a zmes sa mieša v priebehu 1 hodiny. Surová.... ...
Občianske združenie IPASK (Iniciatíva pôrodných asistentiek Slovenska) je dobrovoľnou spoločenskou mimovládnou organizáciou, ktorá vznikla z potreby zviditeľniť a posilniť profesijný status, uplatnenie kompetencií a všetkých rolí pôrodnej asistentky (PA) v praxi ako zdravotníckej profesionálky, ako rovnocenného poskytovateľa zdravotnej starostlivosti s autonómnym a regulovaným povolaním v súlade s právnymi normami SR, direktívami EÚ, odporúčaniami Svetovej zdravotníckej organizácie (WHO) a ostatných medzinárodných zdravotníckych inštitúcií (FIGO, ICM, EMA…)
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Aleutian mink disease virus (AMDV) is widespread among ranched and free-ranging American mink in Canada, but there is no information on its prevalence in other wild animal species. This paper describes the prevalence of AMDV of 12 furbearing species in Nova Scotia (NS), Canada. Samples were collected from carcasses of 462 wild animals of 12 furbearing species, trapped in 10 NS counties between November 2009 and February 2011. Viral DNA was tested by PCR using two primer pairs, and anti-viral antibodies were tested by counterimmunoelectrophoresis (CIEP) on spleen homogenates. Positive PCR or CIEP samples were detected in 56 of 60 (93.3%) American mink, 43 of 61 (70.5%) short-tailed weasels, 2 of 8 (25.0%) striped skunks, 2 of 11 (18.2%) North American river otters, 9 of 85 (10.6%) raccoons, and 2 of 20 (10.0%) bobcats. Samples from six fishers, 24 coyotes, 25 red foxes, 58 beavers, 45 red-squirrels and 59 muskrats were negative. Antibodies to AMDV were detected by CIEP in 16 of 56 (28.6%) mink and one of
Rabbit Thymus Endothelial Cells from Creative Bioarray are isolated from thymus tissue of New Zealand White Rabbit. Rabbit Thymus Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic pre-coated with secondary antibody ...
TY - JOUR. T1 - Immunological identification of Vibrio parahaemolyticus. T2 - Staphylococcal coagglutination test and enzyme-linked immunosorbent assay. AU - Shinoda, Sumio. AU - Ikeda, Mitsuyo. AU - Yamada, Sanae. AU - Kane, Haruaki. AU - Miyoshi, Noriko. PY - 1985. Y1 - 1985. N2 - The species-specific antigenicity of the lateral flagella of Vibrio parahaemolyticus was applied to techniques for immunological identification of the vibrio. One was a coagglutination test using staphylococcal cells sensitized with anti-lateral-flagella antibody bound to protein A located in the staphylococcal cell wall. The other technique was an enzyme-linked immunosorbent assay (ELISA) using a four-layer sandwich procedure. By means of the staphylococcal coagglutination technique, about 106 vibrio cells or 60 ng of flagellar protein could be detected and the whole procedure was performed within 1 h. The sandwich ELISA procedure was more sensitive, but the time required for the whole procedure was about 8 h. Both ...
Definition of latex agglutination test in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is latex agglutination test? Meaning of latex agglutination test as a legal term. What does latex agglutination test mean in law?
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Allergic bronchopulmonary aspergillosis - WikipediaAllergic bronchopulmonary aspergillosis - Wikipedia

Both are more sensitive than conventional counterimmunoelectrophoresis. IgG may not be entirely specific for ABPA, as high ...
more infohttps://en.wikipedia.org/wiki/Allergic_bronchopulmonary_aspergillosis

Aleutian mink disease virus in furbearing mammals in Nova Scotia, Canada | Acta Veterinaria Scandinavica | Full TextAleutian mink disease virus in furbearing mammals in Nova Scotia, Canada | Acta Veterinaria Scandinavica | Full Text

Viral DNA was tested by PCR using two primer pairs, and anti-viral antibodies were tested by counterimmunoelectrophoresis (CIEP ... Counterimmunoelectrophoresis (CIEP) was carried out on duplicate 50 μl samples of cell-free supernatants by the Animal Health ... Viral DNA was tested by PCR using two primer pairs, and anti-viral antibodies were tested by counterimmunoelectrophoresis (CIEP ... Cho HJ, Greenfield J: Eradication of Aleutian disease of mink by eliminating positive counterimmunoelectrophoresis test ...
more infohttps://actavetscand.biomedcentral.com/articles/10.1186/1751-0147-55-10

Counterimmunoelectrophoresis - WikipediaCounterimmunoelectrophoresis - Wikipedia

Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is ... http://medical-dictionary.thefreedictionary.com/counter+immunoelectrophoresis Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. ( ... ISBN 0-8385-8529-9. Counterimmunoelectrophoresis at the US National Library of Medicine Medical Subject Headings (MeSH) http:// ...
more infohttps://en.wikipedia.org/wiki/Counterimmunoelectrophoresis

Serological diagnosis of typhoid fever by counterimmunoelectrophoresis. | The BMJSerological diagnosis of typhoid fever by counterimmunoelectrophoresis. | The BMJ

Serological diagnosis of typhoid fever by counterimmunoelectrophoresis. Br Med J (Clin Res Ed) 1981; 282 :1505 ... An additional advantage of counterimmunoelectrophoresis is that results may be obtained on the same day that the serum ... Less satisfactory results were obtained by counterimmunoelectrophoresis when two other preparations, a protein extract (Barber ... The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella ...
more infohttp://www.bmj.com/content/282/6275/1505

Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method | The Journal...Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method | The Journal...

Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method. John C. ... Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for ... Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method ... Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method ...
more infohttp://www.jimmunol.org/content/112/3/987

The American Journal of Tropical Medicine and Hygiene | Detection of Circulating Parasite Antigen in Bancroftian Filariasis by...The American Journal of Tropical Medicine and Hygiene | Detection of Circulating Parasite Antigen in Bancroftian Filariasis by...

Abstract We used counterimmunoelectrophoresis (CIEP) with rabbit antibodies to Dirofilaria immitis and Brugia malayi to detect ... Detection of Circulating Parasite Antigen in Bancroftian Filariasis by Counterimmunoelectrophoresis * G. J. Weil*, H. Kumar, S ... We used counterimmunoelectrophoresis (CIEP) with rabbit antibodies to Dirofilaria immitis and Brugia malayi to detect soluble ...
more infohttp://www.ajtmh.org/content/journals/10.4269/ajtmh.1986.35.565

Risk of congenital complete heart block in newborns of mothers with anti-Ro/SSA antibodies detected by...Risk of congenital complete heart block in newborns of mothers with anti-Ro/SSA antibodies detected by...

Counterimmunoelectrophoresis and immunoblotting were used to test for antibodies to extractable nuclear antigens. ... of congenital complete heart block in newborns of mothers with anti-Ro/SSA antibodies detected by counterimmunoelectrophoresis ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/11508435?dopt=Abstract

Serogrouping of Legionella pneumophila by double diffusion and counter immunoelectrophoresis. | Journal of Clinical PathologySerogrouping of Legionella pneumophila by double diffusion and counter immunoelectrophoresis. | Journal of Clinical Pathology

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
more infohttp://jcp.bmj.com/content/37/11/1308

The American Journal of Tropical Medicine and Hygiene | Counterimmunoelectrophoresis Using an Arc 5 Antigen for the Rapid...The American Journal of Tropical Medicine and Hygiene | Counterimmunoelectrophoresis Using an Arc 5 Antigen for the Rapid...

Counterimmunoelectrophoresis was performed on a cellulose acetate membrane with dilutions of a commercially available antigen ... 89.9%) were higher with counterimmunoelectrophoresis than with indirect hemagglutination. Cross-reactivity with sera of ... Abstract A modified counterimmunoelectrophoresis and a conventional indirect hemagglutination test were compared for routine ... patients with other parasitic infections was noted with indirect hemagglutination but not with counterimmunoelectrophoresis. ...
more infohttp://www.ajtmh.org/content/journals/10.4269/ajtmh.1987.36.592

Get PDF - Serological detection of infectious bursal diseases virus antibodies in chicken's sera in Western Sudan by...Get PDF - Serological detection of infectious bursal diseases virus antibodies in chicken's sera in Western Sudan by...

Application of counterimmunoelectrophoresis for detection of infectious bursal disease virus antibodies in field serum. Indian ... Rapid detection of infectious bursal disease antibodies by counterimmunoelectrophoresis. Avian Pathology 11(4): 611-614, 1982 ... s sera in Western Sudan by counterimmunoelectrophoresis. E.H.ssan, S.M.; Kheir, S.A.M.; Salim, A.I.. Bulletin of Animal Health ... antibodies by counter-immunoelectrophoresis test, 14.6% of the total samples examined were positive. 19.7%, 9.5% and 5.5% ...
more infohttps://eurekamag.com/research/002/219/002219397.php

HKU Scholars Hub: Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear...HKU Scholars Hub: Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear...

Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in ... Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in ... Article: Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen ... Anti-extractable nuclear antigen (ENA) antibodies were assayed by counter immunoelectrophoresis (CIE) and immunoblotting in ...
more infohttp://hub.hku.hk/handle/10722/162283

Development and application of the counterimmunoelectrophoresis test for the immunodiagnosis of C. fetus infection in cattle.Development and application of the counterimmunoelectrophoresis test for the immunodiagnosis of C. fetus infection in cattle.

The counterimmunoelectrophoresis test (CIE) was developed for the diagnosis of C. fetus infection in cattle. It was positive in ... Development and application of the counterimmunoelectrophoresis test for the immunodiagnosis of C. fetus infection in cattle.. ...
more infohttp://erepository.uonbi.ac.ke:8080/xmlui/handle/11295/66177

Serodiagnosis of the Infectious Diseases | SpringerLinkSerodiagnosis of the Infectious Diseases | SpringerLink

Among the several modes of diagnostic testing for infections, serological (i.e. antibody determination) studies are still heavily used. Serodiagnosis of the Infectious Diseases examines this topic in
more infohttps://link.springer.com/book/10.1007%2F978-1-4615-5249-9

Words that end in ectrophoresis | Words ending in ectrophoresisWords that end in ectrophoresis | Words ending in ectrophoresis

counterimmunoelectrophoresis. 26-letter words that end in ectrophoresis. *radioimmunoelectrophoresis. 22-letter words that end ...
more infohttp://www.thefreedictionary.com/words-that-end-in-ectrophoresis

ArboCat Virus: Tahyna (TAHV)ArboCat Virus: Tahyna (TAHV)

HI, CF, NT, IFA, counter immunoelectrophoresis Footnotes. ,,,Click on the PDF icon to the left to view a copy of this virus ...
more infohttps://wwwn.cdc.gov/Arbocat/VirusDetails.aspx?ID=460&SID=4

An A | Elisa | Systemic Lupus ErythematosusAn A | Elisa | Systemic Lupus Erythematosus

counterimmunoelectrophoresis and double immunodiffusion. Maini RN: The consensus workshops for the detection of auto antibodies ...
more infohttps://www.scribd.com/document/91720131/An-A

Bone hydatid disease | Postgraduate Medical JournalBone hydatid disease | Postgraduate Medical Journal

CIE, counterimmunoelectrophoresis. *CT, computed tomography. *EG, Echinococcus granulosus. *ELISA, enzyme-linked immunosorbent ...
more infohttp://pmj.bmj.com/content/83/982/536

Table of Contents - May 09, 1981, 282 (6275) | The BMJTable of Contents - May 09, 1981, 282 (6275) | The BMJ

Serological diagnosis of typhoid fever by counterimmunoelectrophoresis. Br Med J (Clin Res Ed) 1981; 282 :1505 (Published 09 ...
more infohttp://www.bmj.com/content/282/6275

GBMC Test DictionaryGBMC Test Dictionary

Counterimmunoelectrophoresis (CIE) is a procedure in which oppositely charged antigen and antibody are propelled toward each ...
more infohttp://www.specialtylabs.com/clients/gbmc/methodology.asp?id=WBC

The use of laboratory tests in the diagnosis of SLE | Journal of Clinical PathologyThe use of laboratory tests in the diagnosis of SLE | Journal of Clinical Pathology

A comparison of radioimmunoassay, counterimmunoelectrophoresis and indirect immunofluorescence on Crithidia luciliae substrate ...
more infohttps://jcp.bmj.com/content/53/6/424?ijkey=2da99ead19535c3e5dc7b9491a2cec36f8a8edc1&keytype2=tf_ipsecsha

Metwally D[au] - PubMed - NCBIMetwally D[au] - PubMed - NCBI

Serodiagnosis of giardiasis by counterimmunoelectrophoresis and indirect immunofluorescence tests.. Abdel Hamid MY, Makled KM, ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Metwally+D%5Bau%5D&dispmax=50

Journal of Postgraduate Medicine: Back issue statisticsJournal of Postgraduate Medicine: Back issue statistics

Counterimmunoelectrophoresis in the immunodiagnosis of amoebiasis.. MM Bapat, GG Bhave. July-September 1990, 36(3):124-7. PMID: ... Counterimmunoelectrophoresis (CIE) detection of antiamoebic antibodies in the patients sera, has been carried out and ...
more infohttp://www.jpgmonline.com/showstats.asp?sabs=n&issn=0022-3859
  • Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is similar to immunodiffusion, but with the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel. (wikipedia.org)
  • Less satisfactory results were obtained by counterimmunoelectrophoresis when two other preparations, a protein extract (Barber antigen) and an ultrasonic lysate, were used as the antigens. (bmj.com)
  • It is concluded that in an area where typhoid is endemic counterimmunoelectrophoresis using veronal buffer extract as the antigen is superior to the Widal test for serological diagnosis of typhoid fever. (bmj.com)
  • We used counterimmunoelectrophoresis (CIEP) with rabbit antibodies to Dirofilaria immitis and Brugia malayi to detect soluble filarial antigen in sera collected in a Wuchereria bancrofti -endemic area in South India. (ajtmh.org)
  • Counterimmunoelectrophoresis was performed on a cellulose acetate membrane with dilutions of a commercially available antigen which interacts with sera of patients with confirmed hydatid disease to produce the arc 5 precipitin line. (ajtmh.org)
  • Counterimmunoelectrophoresis (CIE) is a procedure in which oppositely charged antigen and antibody are propelled toward each other by an electrical field. (specialtylabs.com)
  • In the remaining 11 cases, the pneumonia was nonbacteremic, in which the diagnostic criterion was the detection of pneumococcal capsular antigen (PCA) in urine by counterimmunoelectrophoresis (CIE) ( 4 ). (asm.org)
  • A modified counterimmunoelectrophoresis and a conventional indirect hemagglutination test were compared for routine diagnosis of human hydatid disease in an endemic area in the Middle East. (ajtmh.org)
  • Cross-reactivity with sera of patients with other parasitic infections was noted with indirect hemagglutination but not with counterimmunoelectrophoresis. (ajtmh.org)
  • Sensitivity (95.5% vs. 93.2%) and specificity (99.2% vs. 89.9%) were higher with counterimmunoelectrophoresis than with indirect hemagglutination. (ajtmh.org)
  • Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for diagnostic purposes. (jimmunol.org)