Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow.
A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
Antibodies which elicit IMMUNOPRECIPITATION when combined with antigen.
Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Inflammation of the coverings of the brain and/or spinal cord, which consist of the PIA MATER; ARACHNOID; and DURA MATER. Infections (viral, bacterial, and fungal) are the most common causes of this condition, but subarachnoid hemorrhage (HEMORRHAGES, SUBARACHNOID), chemical irritation (chemical MENINGITIS), granulomatous conditions, neoplastic conditions (CARCINOMATOUS MENINGITIS), and other inflammatory conditions may produce this syndrome. (From Joynt, Clinical Neurology, 1994, Ch24, p6)
Substances elaborated by bacteria that have antigenic activity.
Infections of the nervous system caused by bacteria of the genus HAEMOPHILUS, and marked by prominent inflammation of the MENINGES. HAEMOPHILUS INFLUENZAE TYPE B is the most common causative organism. The condition primarily affects children under 6 years of age but may occur in adults.
Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.
Viruses whose taxonomic relationships have not been established.
Immunoglobulins produced in a response to FUNGAL ANTIGENS.
A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals.
A species of HAEMOPHILUS found on the mucous membranes of humans and a variety of animals. The species is further divided into biotypes I through VIII.
Alkaloids extracted from various species of Cinchona.
Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)
A slow progressive disease of mink caused by the ALEUTIAN MINK DISEASE VIRUS. It is characterized by poor reproduction, weight loss, autoimmunity, hypergammaglobulinemia, increased susceptibility to bacterial infections, and death from renal failure. The disease occurs in all color types, but mink which are homozygous recessive for the Aleutian gene for light coat color are particularly susceptible.
Studies determining the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. For drugs and devices, CLINICAL TRIALS AS TOPIC; DRUG EVALUATION; and DRUG EVALUATION, PRECLINICAL are available.
A species of PARVOVIRUS that causes a disease in mink, mainly those homozygous for the recessive Aleutian gene which determines a desirable coat color.
A bacterium which causes mastitis in cattle and occasionally in man.
Immunoelectrophoresis in which a second electrophoretic transport is performed on the initially separated antigen fragments into an antibody-containing medium in a direction perpendicular to the first electrophoresis.
Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.
Antigens of the virion of the HEPATITIS B VIRUS or the Dane particle, its surface (HEPATITIS B SURFACE ANTIGENS), core (HEPATITIS B CORE ANTIGENS), and other associated antigens, including the HEPATITIS B E ANTIGENS.
Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.
A febrile disease caused by STREPTOCOCCUS PNEUMONIAE.
A species of gram-negative, aerobic BACTERIA. It is a commensal and pathogen only of humans, and can be carried asymptomatically in the NASOPHARYNX. When found in cerebrospinal fluid it is the causative agent of cerebrospinal meningitis (MENINGITIS, MENINGOCOCCAL). It is also found in venereal discharges and blood. There are at least 13 serogroups based on antigenic differences in the capsular polysaccharides; the ones causing most meningitis infections being A, B, C, Y, and W-135. Each serogroup can be further classified by serotype, serosubtype, and immunotype.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Bacterial polysaccharides that are rich in phosphodiester linkages. They are the major components of the cell walls and membranes of many bacteria.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
An acute systemic febrile infection caused by SALMONELLA TYPHI, a serotype of SALMONELLA ENTERICA.
A serotype of SALMONELLA ENTERICA which is the etiologic agent of TYPHOID FEVER.
Species of the genus MASTADENOVIRUS, causing a wide range of diseases in humans. Infections are mostly asymptomatic, but can be associated with diseases of the respiratory, ocular, and gastrointestinal systems. Serotypes (named with Arabic numbers) have been grouped into species designated Human adenovirus A-F.
Specific hemagglutinin subtypes encoded by VIRUSES.
The branch of medicine concerned with diseases, mainly of parasitic origin, common in tropical and subtropical regions.
An infection caused by the infestation of the larval form of tapeworms of the genus Echinococcus. The liver, lungs, and kidney are the most common areas of infestation.
Electrophoresis in which cellulose acetate is the diffusion medium.
It is a form of protection provided by law. In the United States this protection is granted to authors of original works of authorship, including literary, dramatic, musical, artistic, and certain other intellectual works. This protection is available to both published and unpublished works. (from Circular of the United States Copyright Office, 6/30/2008)
A subspecialty of pathology applied to the solution of clinical problems, especially the use of laboratory methods in clinical diagnosis. (Dorland, 28th ed.)
A species of gram-negative, aerobic bacteria that is the causative agent of LEGIONNAIRES' DISEASE. It has been isolated from numerous environmental sites as well as from human lung tissue, respiratory secretions, and blood.
Gram-negative aerobic rods, isolated from surface water or thermally polluted lakes or streams. Member are pathogenic for man. Legionella pneumophila is the causative agent for LEGIONNAIRES' DISEASE.
An acute, sometimes fatal, pneumonia-like bacterial infection characterized by high fever, malaise, muscle aches, respiratory disorders and headache. It is named for an outbreak at the 1976 Philadelphia convention of the American Legion.
Hypersensitivity reaction (ALLERGIC REACTION) to fungus ASPERGILLUS in an individual with long-standing BRONCHIAL ASTHMA. It is characterized by pulmonary infiltrates, EOSINOPHILIA, elevated serum IMMUNOGLOBULIN E, and skin reactivity to Aspergillus antigen.
A species of imperfect fungi from which the antibiotic fumigatin is obtained. Its spores may cause respiratory infection in birds and mammals.
Infections with fungi of the genus ASPERGILLUS.
Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).
A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
Pulmonary diseases caused by fungal infections, usually through hematogenous spread.
Branch-like terminations of NERVE FIBERS, sensory or motor NEURONS. Endings of sensory neurons are the beginnings of afferent pathway to the CENTRAL NERVOUS SYSTEM. Endings of motor neurons are the terminals of axons at the muscle cells. Nerve endings which release neurotransmitters are called PRESYNAPTIC TERMINALS.
Lists of words to which individuals are asked to respond ascertaining the conceptual meaning held by the individual.
The sum or the stock of words used by a language, a group, or an individual. (From Webster, 3d ed)
Skeletal muscle structures that function as the MECHANORECEPTORS responsible for the stretch or myotactic reflex (REFLEX, STRETCH). They are composed of a bundle of encapsulated SKELETAL MUSCLE FIBERS, i.e., the intrafusal fibers (nuclear bag 1 fibers, nuclear bag 2 fibers, and nuclear chain fibers) innervated by SENSORY NEURONS.
The relationships between symbols and their meanings.
Cells specialized to transduce mechanical stimuli and relay that information centrally in the nervous system. Mechanoreceptor cells include the INNER EAR hair cells, which mediate hearing and balance, and the various somatosensory receptors, often with non-neural accessory structures.
Text editing and storage functions using computer software.

Immunodiagnosis of histoplasmosis in a compromised host. (1/267)

Three serological tests for the diagnosis of histoplasmosis were compared for sensitivity and specificity in serum from blood bank donors, patients with histoplasmosis, and infected or noninfected immunosuppressed patients. The histoplasmin latex agglutination test was positive in 9% of the normal patients, 33% of the histoplasmosis patients, and 61% of the noninfected immunosuppressed patients. Since the test is prone to many false-positive results in patients with inflammatory diseases or non-Histoplasma infections, it has limited potential as a screening test among compromised patients. Immunodiffusion and counterimmunoelectrophoresis using a mycelial antigen were found to be more sensitive than either test using a combined yeast and mycelial antigen or a pure yeast phase antigen. Counterimmunoelectrophoresis at pH 7.2 proved to be the test of choice for serodiagnosis of histoplasmosis, resolving 85% of the immunocompetent infected patients and 100% of the infected immunosuppressed patients. Results indicated that counterimmunoelectrophoresis in conjunction with immunodiffusion could be used as a screening protocol to determine infection in incoming patients in a cancer hospital.  (+info)

Counterimmunoelectrophoretic detection of a high incidence of precipitin reactions in normal human sera against staphylococcal teichoic acids and protein A. (2/267)

The use of counterimmunoelectrophoresis (CIE) for detection of serum antibodies to staphylococcal teichoic acids was evaluated against teichoic acids prepared by sonic treatment or lysostaphin extraction of Staphylococcus aureus (Lafferty strain). Of 54 patient sera from suspected cases of staphylococcal endocarditis, osteomyelitis, or septicemia, 33 (61.1%) were positive by CIE analysis; however, 128 of 291 sera (44.0%) from normal adult donors were also positive. Selected CIE-positive sera from patient and control groups were titered by Ouchterlony gel diffusion. In the control group of normal sera, 65% were also positive by gel diffusion, but only 15% had titers of >/=1:2. Of the patient sera, 44.4% had gel diffusion titers of >/=1:2. In addition to the specific teichoic acid band, a second precipitation band could be demonstrated with both patient or normal sera by CIE or gel diffusion. This second precipitin band was shown to involve interactions of test sera with staphylococcal protein A present in the teichoic acid extracts. The protein A precipitins were detected at high concentrations of the antigen extracts, whereas the anti-teichoic acid precipitins were optimally detected at lower antigen concentrations. The formation of protein A precipitin bands did not correlate with the presence of anti-teichoic acid antibodies, as most sera tested were positive for protein A regardless of anti-teichoic acid activity. This study suggests that a high incidence of normal people have levels of antibodies to teichoic acids which are detectable by the highly sensitive, but nonspecific, technique of CIE.  (+info)

Detection of pneumolysin in sputum. (3/267)

Western blot detection of the species-specific pneumococcal product, pneumolysin (SPN), was shown to be almost as sensitive as PCR for the non-cultural detection of pneumococci in 27 Streptococcus pneumoniae culture-positive sputa from patients stated to have chest infections. Both techniques were considerably more sensitive than counter-current immuno-electrophoresis for pneumococcal capsular polysaccharide antigens (CPS-CIE) on the same specimens. Sensitivities for PCR, SPN-immunoblotting and CPS-CIE were 100%, 85% and 67%, respectively. In 11 S. pneumoniae culture-negative sputa taken from patients receiving antibiotics, but with proven recent pneumococcal infection, PCR and SPN-blot were positive in six (in two of which CPS-CIE was also positive), PCR alone was positive in one and SPN-blot alone was positive in one. In 11 S. pneumoniae culture-negative samples from patients not receiving antibiotics, all three tests were negative in eight, PCR was positive in three (in one of which CPS-CIE was also positive), but SPN-blot was negative in all 11. In 16 S. pneumoniae culture-negative samples from patients receiving antibiotics and with no known recent pneumococcal infections, one or more non-cultural test was positive in 11. Although further evaluation is required to assess the significance of pneumolysin detection in relation to carriage and infection and to devise a more suitable test format, these preliminary studies suggest that pneumolysin detection is a promising new approach to the non-cultural diagnosis of pneumococcal chest infection.  (+info)

Pneumococcal antigen in pneumonia. A post-mortem study with the histological and bacteriological findings. (4/267)

Pneumococcal capsular antigens can be detected in lung tissue by counter-current immunoelectrophoresis even when, following antibiotics, post-mortem bacterilogy suggests that Escherichia coli has replaced pneumococci. The results suggest that antipneumococcal therapy would benefit at least 55% of patients critically ill with lung infection and that the potentially toxic drugs directed at coliform bacteria may be unnecessary.  (+info)

Evaluation of tests for antibody response in the follow-up of patients with acute and chronic forms of paracoccidioidomycosis. (5/267)

Several serological tests have been used successfully in the diagnosis of paracoccidioidomycosis (PCM). In contrast, data about the use of these tests in the follow-up of PCM patients have been heterogeneous. In this study, serum samples from 43 PCM patients with different clinical forms were analysed by counter-immuno-electrophoresis (CIE), complement fixation (CF) and ELISA before treatment. With CIE and ELISA, the chronic unifocal form showed significantly lower antibody levels compared with chronic multifocal and acute forms. Acute form patients had significantly higher titres than patients with multifocal disease by CIE but not by ELISA. No significant differences were observed with CF. Twenty-seven of these patients were followed-up for 2 years and showed a decline in antibody levels by all three tests, paralleling clinical improvement. However, only patients with unifocal disease cleared their antibodies after 1 year of treatment as analysed by CF and ELISA and after 2 years by CIE, suggesting that these patients may need shorter courses of therapy. Patients with the other clinical form of the disease needed > or =2 years of therapy to clear their antibodies. Sera from a further five patients who presented with a relapse were analysed. At the time of relapse all showed increases in antibody levels by CIE and ELISA, but only three showed increases by CF tests. Therefore, CIE and ELISA demonstrated a better clinical correlation than CF, probably reflecting the fungal burden of PCM patients more accurately.  (+info)

Alkyl hydroperoxide reductases C and D are major antigens constitutively expressed by Mycobacterium avium subsp. paratuberculosis. (6/267)

Antigens characteristic for Mycobacterium avium subspecies paratuberculosis were identified by crossed immunoelectrophoresis (CIE) and by absorbing out cross-reactive antigens by using a polyclonal and polyvalent Mycobacterium avium subspecies avium antiserum. Two antigens were present in M. avium subsp. paratuberculosis and not detected in Mycobacterium avium subsp. avium. They were identified as antigens 17 and 20 in a CIE reference system for M. avium subsp. paratuberculosis antigens. Purified antigen 20 was identified as alkyl hydroperoxide reductase C (AhpC) while the N-terminal part of purified antigen 17 showed 80% homology with alkyl hydroperoxide reductase D (AhpD) of Mycobacterium tuberculosis. AhpC had a nonreduced mobility in sodium dodecyl sulfate-polyacrylamide gel electrophoresis corresponding to a molecular mass of 45 kDa and is probably a homodimer linked with disulfide bridges in its native form. AhpD had a mobility corresponding to 19 kDa. Monospecific rabbit antiserum against AhpC and AhpD reacted with 9 strains of M. avium subsp. paratuberculosis but not with 20 other mycobacterial strains except for a Mycobacterium gordonae strain, against which a weak cross-reactive band was produced. Goats experimentally infected with M. avium subsp. paratuberculosis had strong gamma interferon (IFN-gamma) responses toward both AhpC and AhpD, and they also had antibodies against AhpC. The ability of AhpC and AhpD to induce IFN-gamma production shows that these proteins potentially could be used in future vaccines or in diagnostic assays. These results further show that AhpC and AhpD are immunologically important proteins which are constitutively and highly expressed in M. avium subsp. paratuberculosis without the bacteria being submitted to oxidative stress and that the specificities of antigens can be a matter of different levels of protein expression in various species as well as distinct structural differences.  (+info)

Immune response in cattle vaccinated against rabies. (7/267)

In order to determine the best type of rabies vaccine to use as a booster, 78 serological samples from singly vaccinated cattle were analyzed by counterimmunoelectrophoresis technique. The animals were divided into several groups, received the first vaccine dose with modified live virus vaccine (ERA strain) and were revaccinated with inactivated virus or modified live virus vaccines. Boosters were given at 2, 4, 8, 12 and 16 weeks following first vaccination. Results showed high titres in the cases of booster with inactivated vaccine. In all cases, however, detectable antibody titres declined quickly.  (+info)

Canine experimental infection: intradermal inoculation of Leishmania infantum promastigotes. (8/267)

Five mixed breed dogs were inoculated intradermally (ID) with cultured virulent stationary phase promastigotes of Leishmania infantum Nicole, 1908 stocks recently isolated. Parasite transformations in the skin of ID infected dogs were monitored from the moment of inoculation and for 48 h, by skin biopsies. Anti-Leishmania antibody levels were measured by indirect immunofluorescence assay, counterimmunoelectrophoresis and direct agglutination test, and clinical conditions were examined. Thirty minutes after ID inoculation the first amastigotes were visualised and 3 to 4 h after inoculation the promastigotes were phagocytized by neutrophils and by a few macrophages. These cells parasitised by amastigotes progressively disappeared from the skin and 24 h after inoculation parasites were no longer observed. Local granulomes were not observed, however, serological conversion for antibodies anti-Leishmania was achieved in all dogs. Direct agglutination test was the only technique positive in all inoculated dogs. Amastigotes were found in the popliteal lymph node in one dog three months after inoculation. This work demonstrates that, with this inoculum, the promastigotes were transformed into amastigotes and were up taken by neutrophils and macrophages. The surviving parasites may have been disseminated in the canine organism, eliciting a humoral response in all cases.  (+info)

Abstract A modified counterimmunoelectrophoresis and a conventional indirect hemagglutination test were compared for routine diagnosis of human hydatid disease in an endemic area in the Middle East. Counterimmunoelectrophoresis was performed on a cellulose acetate membrane with dilutions of a commercially available antigen which interacts with sera of patients with confirmed hydatid disease to produce the arc 5 precipitin line. The test was performed with unconcentrated human sera and the lines stained in an aqueous solution of Ponceau red. Sensitivity (95.5% vs. 93.2%) and specificity (99.2% vs. 89.9%) were higher with counterimmunoelectrophoresis than with indirect hemagglutination. Cross-reactivity with sera of patients with other parasitic infections was noted with indirect hemagglutination but not with counterimmunoelectrophoresis. There was no cross-reactivity with sera of patients with autoimmune disorders by either test.
The complex antigenic properties of adenovirus-soluble complete and incomplete hemagglutinins were utilized to study the specificity of adenovirus anti-hemagglutinin sera in immunodiffusion and counterimmunoelectrophoresis tests. Pentons, penton dimers, fiber dimers, and/or dodecons of adenovirus types 2, 3, 5, 7a, 8, and 11 were extensively purified by ion-exchange and exclusion chromatographies. Components were determined to be purified proteins by immunologic, electron microscopic, and polyacrylamide gel electrophoretic criteria. Specific antigenic components were identified by immunoelectrophoresis and by various biologic characteristics. Rabbit antisera prepared against these hemagglutinating components were type-specific in standard serologic, Ouchterlony immunodiffusion, immunoelectrophoretic, and counterimmunoelectrophoretic tests. Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for diagnostic purposes.. ...
The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella typhi were compared with those of the Widal test in the serological diagnosis of typhoid fever. A veronal buffer extract yielded precipitation lines against 50 (96%) out of 52 sera collected from patients with typhoid but against none out of 62 sera obtained from control subjects who did not have typhoid. Less satisfactory results were obtained by counterimmunoelectrophoresis when two other preparations, a protein extract (Barber antigen) and an ultrasonic lysate, were used as the antigens. By Widal test the rate of detection in patients sera was 73% and the false-positive rate for control sera 16%. It is concluded that in an area where typhoid is endemic counterimmunoelectrophoresis using veronal buffer extract as the antigen is superior to the Widal test for serological diagnosis of typhoid fever. An additional advantage of counterimmunoelectrophoresis is that results ...
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To ascertain the heterogeneity of anti-prothrombin antibodies (aPT), we compared three in-house aPT ELISAs: A) medium binding plates, phosphatidylserine, prothrombin, Tris-buffered saline, calcium; B) high binding plates, prothrombin, Tris-buffered saline; and C) high binding plates, prothrombin, PBS. One serum, exhibiting high positive aPT in all three ELISAs, was selected as the calibrator. Sera from 47 patients (41 with SLE, 4 with pAPS, 2 with arterial thromboses) were tested for IgG and IgM aPT.. The results showed six different patterns: 1) similar results were obtained with A, B and C (similar analytical sensitivity); 2) similar results were obtained with A and B while C showed lower analitycal sensitivity; 3) B and C seemed analytically less sensitive than A; 4) A and B were analytically less sensitive than C; 5) A showed very low analytical sensitivity; 6) A and C showed lower analytical sensitivity than B.. The analysis of all the presented patterns showed noncomparable results of the ...
Anti-Prothrombin antibody conjugated to Biotin validated for WB, IP, RIA, cELISA and tested in Human. Immunogen corresponding to full length native protein…
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Haemophilus influenzae type B Antigen Detection, LA; Neisseria meningitidis Antigen Detection (Groups A/Y and C/W 135), LA; Neisseria meningitidis Detection (Group B/E. coli K1), LA; Streptococcus, Group B, Antigen Detection, LA; Streptococcus pneumoniae Antigen Detection, LA ...
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GCE CIE Biology - In this article we will explain the primary structure, secondary structure, tertiary structure and quaternary structure of proteins, and also describe the four ma
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Notes for the CIE O Level Chemistry module: 2. The particulate nature of matter. These have been made according to the specifications 5070 (2017-2019) and cover all the relevant topics for examination in May/June.. CIE O Level 2. The particulate nature of matter Notes:. ...
Witam, ostatnio skanowałem sobie system programem Malwarebytes Anti-Malware i wykrył mi on szkodniki w systemie. Wszystkie oczywiście usunął, natomiast dodatkowo chciałbym prosić szanowni forumowicze żebyście dokonali …
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The SET-RPLA, from Denka Seiken Co. Ltd., Tokio, a commercial reversed passive latex agglutination test kit, has been recommended to establish the enterotoxicity capacity of some staphylococcal strains, implicated in food poisoning outbreaks that produce low levels of enterotoxins (SE). Despite the RPLA specificity, the occurrence of nonspecific reactions when testing low- SE-producing is common. In order to control these nonspecific reactions the addition of purified normal rabbit IgG purified was applied on approximately 350 staphylococcal isolates from human milk and anatomic sites of healthy dental student carriers. The results indicated that addition of 5% (v/v) of purified normal rabbit IgG (0.74 mg/mL) to the culture supernatant fluid is a simple and reliable tool for the controlling of nonspecific reactions in the RPLA assay ...
In a series of 163 ears with chronic, mucoid effusion, free or immune complex bound pneumococcal capsular polysaccharide antigens were demonstrated with Omniserum and counterimmunoelectrophoresis (CIEP) in 37 ears (23%). In crushed adenoid tissue thi
Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV. The results were compared to those obtained for 75 non-HIV-infected patients with endemic paracoccidioidomycosis. Anti-P. brasiliensis antibodies were detected in 65% (DID), 79% (CIEP) and 95% (ELISA) of the patients with HIV/AIDS, significantly lower rates than those detected in cases of endemic paracoccidioidomycosis, which were 89%, 99% and 100%, respectively. The reactive sera of HIV-infected patients also showed lower anti-P. brasiliensis antibody ...
Bruder RC et al. The importance of counterimmunoelectrophoresis in the detection of extractable nuclear antigens for the diagnosis of systemic rheumatic diseases. J. Bras. Patol. Med. Lab. vol.40 no.1 Rio de Janeiro Feb. 2004. Autoimmune Manifestations in Acute Viral Hepatitides ...
TY - JOUR. T1 - La sierologia del Lupus eritematoso. AU - Parodi, A.. AU - Cozzani, E.. AU - Drosera, M.. PY - 2004. Y1 - 2004. N2 - Lupus erythematosus (LE), expecially its systemic form (SLE), is serologically characterized by the presence of autoantibodies directed to nuclear, cytoplasmic and cellular membrane antigens. Some of them are specific of the disease, others characterize only particular substes. Antinuclear antibodies (ANA) can be detected by various assays: indirect immunofluorescence (IIF), immunodiffusion (ID), counterimmunoelectrophoresis (CIE), enzyme linked immunosorbent assay (ELISA), radioimmunoassay (RIA) and immunoblotting (IB). IIF and ELISA are the most used. ELISA is very sensitive, IIF is sensitive, easy to perform and cheap. In IIF monolayers of cultured cells particularly Hep-2 are the best substrate to use. Antibodies to ds-DNA are high specific of SLE and can be used to monitor the clinical course of the disease. Anti-nucleosome antibodies are now considered the ...
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Definition : Serology reagents intended to detect antibodies to teichoic acid, a polymer found in the cell wall and cell membrane of some bacteria. High levels of teichoic acid antibodies in serum indicate the possible presence of bacteria, especially occult Staphylococcus aureus infection.. Entry Terms : Teichoate Antibody Detection/Identification Reagents , Teichoic Acid Antibody Detection/Identification Reagents , Reagents, Serology, Bacteria, Teichoic Acid, Antibody. UMDC code : 17396 ...
Gresca CCIE Redirecting and Changing is the most well-known certification within the CCIE monitor. It is worldwide recognized as probably the most authoritative abilities in the THIS industry. This particular Cisco certification verifies the actual candidates capability to plan, handle and troubleshoot complex business network infrastructures. The CCIE R&S is made for network technicians of the Professional level who are able to detect regions of vulnerabilities as well as develop ones theoretical information as well as useful skills. This specific certification is actually aimed at designers and applicants looking for the particular Cisco qualifications including CCNA/CCNP Service provider or even CCNP R&S or CCIE R&S V5 or CCIE Service Provider V3. LSAT dumps Ms Certified Expert (MCP) is really a gateway towards the certifications better level. It is sometimes considered as the very first stop on the path to the Microsof company Certified Techniques Engineer (MCSE) or microsoft Certified ...
Group members: Dr. Frida Piper, Dr. Alex Fajardo. Within the line of research, Terrestrial Ecosystems of the CIEP, diverse analyses are done associated with the research projects. The processing and analysis of vegetative matter demands effort and time. The process, from the moment the plants are gathered in the field to when they are converted into data with statistical value, requires a high investment in specialized human capital. For this reason, and because these are contextualized in projects of scientific research, the vegetal analyses done at the CIEP provide an opportunity to develop theses for masters degrees and PhDs. The aforementioned analyses are not offered as services.. Entry and processing of vegetative material. The plants or vegetal tissues are generally collected in the field, rarely coming from controlled conditions (nurseries, greenhouses). The first step consists of drying the material, then weighing it, and finally grinding it. For this purpose, the laboratory has a stove ...
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Developments in spectroscopic analysis and routine availability of X-ray crystallography have simplified structural elucidation of natural compounds. However, isolating bioactive components of interest from crude extracts of either plants or animals has always been challenging for two reasons: these extracts contain hundreds of compounds with a range of polarities, and the bioactivity of the compound of interest should be maintained throughout the purification process. This is where countercurrent chromatography (CCC) is helpful.
Prednášky pre 6.ročník „Zubné lekárstvo zimný semester ak. Rok 2016/2017 Prednášky: 1. - 7., 9. - 13. týždeň „Maxilofaciálna chirurgia, poslucháreň Heydukova (utorok 16.30 - 18.20 hod.) 1. Dentoalveolárna chirurgia - opakovanie (anatómia dutiny ústnej, lokálna anestézia, indikácie, komplikácie lokálne, celkové, Extrakcie zubov, indikácie, technika, komplikácie lokálne, endodonticko- chirurgické ošetrenie devitálnych zubov, indikácie, technika, rtg) 2. Dentoalveolárna chirurgia - opakovanie (tretie moláre, vyšetrenie, indikácie extrakcie, asymptomatický tretí molár, technika extrakcie, chirurgická extrakcia, komplikácie, pooperačná starostlivosť) 3. Dentálna implantológia (princípy, základné typy dentálnych implantátov, chirurgická technika, indikácie, protetická sanácia defektov na dentálnych implantátoch) 4. Pacient na antiagregačnej a antikoagulačnej liečbe ( princípy a typy antiagregačnej a antikoagulačnej liečby, ...
Táto práca preukázala, že pravidelná fyzická aktivita strednej intenzity spôsobuje významné zmeny lipoproteínového profilu už v priebehu prvých 14 dní. Priaznivý efekt cvičenia na lipidový metabolizmus bol zdokumentovaný mnohými prácami. Tieto sa však líšili významne dlhším trvaním tréningového protokolu (2 mesiace až 1 rok) [10- 13,15]. Sledovanie zmien v takto krátkom období doposiaľ nebolo v tomto rozsahu v nami dostupnej literatúre realizované. Výrazné zmeny sme pozorovali v oblasti lipoproteínov bohatých na triacylglycerol (VLDL, IDL). Naša práca preukázala štatisticky významné zníženie koncentrácie VLDL v priebehu 14 dní. V literatúre je opísané zníženie koncentrácie VLDL a triacylglycerolu (TAG) pôsobením tréningového programu s využitím aeróbneho cvičenia strednej intenzity o trvaní 6 mesiacov [11,13,15]. K najmarkantnejšiemu zníženiu koncentrácie VLDL dochádza podľa literatúry u jedincov s ...
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Anyhow a couple of months ago my CCIE study came to an end not because I passed the lab unfortunatly but because the kids were to much of a handful for my wife to handle on her own on weekends. I do hope that not everything has fallen out of my head and that I can pick it up again sometime in the near future maybe in winter where I can trick my kids into believing it is bed time at 3pm in the afternoon :-) It will not be any time soon though because my lab is in pieces as I have decided to move house. Not only have I decided to move into a bigger more expensive house when interest rates are at their lowest levels in hundreds of years but I have also decided to move jobs and take a pay cut at the same time. Nothing like making life difficult for yourself hey? As you can see I am going stuggle with that CCIE as I am not the brightest lamp on the street ...
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CIE 10 PREMIUM線上破解APP新玩法懶人包,詳細解說醫療APP手遊最新APP消息,不能錯過全世界Android App Store、iOS App Store、Windows App Store搶先資訊ICD 10 Indonesia-English app,idc 10熱門APP載點排行榜,idc 10 pinLa CIE10 es el acrónimo de la Clasificación internacional de enfermedades, décima versión correspondiente a la versión en español de la (en inglés) IC
OBJETIVOS: Avaliar as alterações clínicas da superfície ocular, citologia de impressão (CI) e sintomas de olho seco (OS) dos pacientes com Espondilite Anquilosante (EA) e Artrite Reumatoide (AR). Classificar...
Agentúra ECHA podniká kroky na zlepšenie kvality informácií v registračných dokumentáciách s cieľom splniť svoj prvý strategický cieľ, ktorým je „maximalizovať dostupnosť vysokokvalitných údajov na umožnenie bezpečnej výroby a používania chemických látok. V rámci týchto krokov agentúra ECHA využíva skúsenosti získané z hodnotenia registračných dokumentácií a časté nedostatky premieta do počítačových algoritmov, ktoré sa môžu použiť na kontrolu celej databázy. To umožňuje agentúre získať lepší prehľad o kvalite registračných údajov a pomáha jej účinnejšie riešiť zistené nezrovnalosti.. Výsledky pôvodných kontrol sa oznamujú registrujúcim prostredníctvom neformálnych listov, ktoré takisto obsahujú informácie o tom, ako odstrániť možné nezrovnalosti. Agentúra očakáva, že spoločnosti budú brať tieto listy vážne a spontánne aktualizovať svoje registrácie, pričom vyriešia zdôraznené výhrady a tieto ...
Double immunodiffusion (DID) and counterimmunoelectrophoresis (CIEP), two forms of gel-based techniques, are used to gain ... Bunn, C.; Kveder, T. (1996). "Counterimmunoelectrophoresis and immunodiffusion for the detection of antibodies to soluble ... and counterimmunoelectrophoresis (CIEP). Both of these techniques require the precipitation of antigens for valid results. ...
... of congenital complete heart block in newborns of mothers with anti-Ro/SSA antibodies detected by counterimmunoelectrophoresis ...
When evidence of ADV shows in a ferret, it is strongly recommended that a CEP (counterimmunoelectrophoresis) blood test or an ...
... counterimmunoelectrophoresis MeSH H01.181.529.307.437.568.520 - two-dimensional immunoelectrophoresis MeSH H01.181.529.307. ...
... counterimmunoelectrophoresis MeSH E05.478.605.492.350.350.350 - immunoelectrophoresis, two-dimensional MeSH E05.478.605.825 - ... counterimmunoelectrophoresis MeSH E05.196.401.568.520 - immunoelectrophoresis, two-dimensional MeSH E05.196.401.663 - ...
... counterimmunoelectrophoresis MeSH E01.450.495.735.645.350.350.350 - immunoelectrophoresis, two-dimensional MeSH E01.450.495.735 ...
... is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is ... ISBN 978-0-8385-8529-0. Counterimmunoelectrophoresis at the US National Library of Medicine Medical Subject Headings (MeSH) ... http://medical-dictionary.thefreedictionary.com/counter+immunoelectrophoresis Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. ( ...
The two most commonly used methods of confirming allergen sensitization are skin testing and allergy blood testing. Both methods are recommended by the NIH guidelines and have similar diagnostic value in terms of sensitivity and specificity.[1][2] Advantages of the allergy blood test range from: excellent reproducibility across the full measuring range of the calibration curve, it has very high specificity as it binds to allergen specific IgE, and extremely sensitive too, when compared with skin prick testing. In general, this method of blood testing (in-vitro, out of body) vs skin-prick testing (in-vivo, in body) has a major advantage: it is not always necessary to remove the patient from an anthihistamine medication regimen, and if the skin conditions (such as eczema) are so widespread that allergy skin testing cannot be done. Allergy blood tests, such as ImmunoCAP, are performed without procedure variations, and the results are of excellent standardization.[3] Adults and children of any age ...
Once the solid substrate bead technology has been chosen, antibodies are coupled to the beads and the antibody-coated-beads can be added to the heterogeneous protein sample (e.g. homogenized tissue). At this point, antibodies that are immobilized to the beads will bind to the proteins that they specifically recognize. Once this has occurred the immunoprecipitation portion of the protocol is actually complete, as the specific proteins of interest are bound to the antibodies that are themselves immobilized to the beads. Separation of the immunocomplexes from the lysate is an extremely important series of steps, because the protein(s) must remain bound to each other (in the case of co-IP) and bound to the antibody during the wash steps to remove non-bound proteins and reduce background. When working with agarose beads, the beads must be pelleted out of the sample by briefly spinning in a centrifuge with forces between 600-3,000 x g (times the standard gravitational force). This step may be ...
A latex fixation test, also called a latex agglutination assay or test (LA assay or test), is an assay used clinically in the identification and typing of many important microorganisms. These tests use the patient's antigen-antibody immune response. This response occurs when the body detects a pathogen and forms an antibody specific to an identified antigen (a protein configuration) present on the surface of the pathogen. Agglutination tests, specific to a variety of pathogens, can be designed and manufactured for clinicians by coating microbeads of latex with pathogen-specific antigens or antibodies. In performing a test, laboratory clinicians will mix a patient's cerebrospinal fluid, serum or urine with the coated latex particles in serial dilutions with normal saline (important to avoid the prozone effect) and observe for agglutination (clumping). Agglutination of the beads in any of the dilutions is considered a positive result, confirming either that the patient's body has produced the ...
The direct method is a one-step staining method and involves a labeled antibody (e.g. FITC-conjugated antiserum) reacting directly with the antigen in tissue sections. While this technique utilizes only one antibody and therefore is simple and rapid, the sensitivity is lower due to little signal amplification, in contrast to indirect approaches.[4] However, this strategy is used less frequently than its multi-phase counterpart. The indirect method involves an unlabeled primary antibody (first layer) that binds to the target antigen in the tissue and a labeled secondary antibody (second layer) that reacts with the primary antibody. As mentioned above, the secondary antibody must be raised against the IgG of the animal species in which the primary antibody has been raised. This method is more sensitive than direct detection strategies because of signal amplification due to the binding of several secondary antibodies to each primary antibody if the secondary antibody is conjugated to the ...
Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is ... ISBN 978-0-8385-8529-0. Counterimmunoelectrophoresis at the US National Library of Medicine Medical Subject Headings (MeSH) ... http://medical-dictionary.thefreedictionary.com/counter+immunoelectrophoresis Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. ( ...
Serological diagnosis of typhoid fever by counterimmunoelectrophoresis. Br Med J (Clin Res Ed) 1981; 282 :1505 ... An additional advantage of counterimmunoelectrophoresis is that results may be obtained on the same day that the serum ... Less satisfactory results were obtained by counterimmunoelectrophoresis when two other preparations, a protein extract (Barber ... The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella ...
Counterimmunoelectrophoresis and immunoblotting were used to test for antibodies to extractable nuclear antigens. ... of congenital complete heart block in newborns of mothers with anti-Ro/SSA antibodies detected by counterimmunoelectrophoresis ...
Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method. John C. ... Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for ... Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method ... Counterimmunoelectrophoresis with Adenovirus Type-Specific Anti-Hemagglutinin Sera As a Rapid Diagnostic Method ...
Abstract We used counterimmunoelectrophoresis (CIEP) with rabbit antibodies to Dirofilaria immitis and Brugia malayi to detect ... Detection of Circulating Parasite Antigen in Bancroftian Filariasis by Counterimmunoelectrophoresis * G. J. Weil*, H. Kumar, S ... We used counterimmunoelectrophoresis (CIEP) with rabbit antibodies to Dirofilaria immitis and Brugia malayi to detect soluble ...
Counterimmunoelectrophoresis was performed on a cellulose acetate membrane with dilutions of a commercially available antigen ... 89.9%) were higher with counterimmunoelectrophoresis than with indirect hemagglutination. Cross-reactivity with sera of ... Abstract A modified counterimmunoelectrophoresis and a conventional indirect hemagglutination test were compared for routine ... patients with other parasitic infections was noted with indirect hemagglutination but not with counterimmunoelectrophoresis. ...
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
Application of counterimmunoelectrophoresis for detection of infectious bursal disease virus antibodies in field serum. Indian ... Rapid detection of infectious bursal disease antibodies by counterimmunoelectrophoresis. Avian Pathology 11(4): 611-614, 1982 ... s sera in Western Sudan by counterimmunoelectrophoresis. E.H.ssan, S.M.; Kheir, S.A.M.; Salim, A.I.. Bulletin of Animal Health ... antibodies by counter-immunoelectrophoresis test, 14.6% of the total samples examined were positive. 19.7%, 9.5% and 5.5% ...
Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in ... Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen antibodies in ... Article: Comparison of counter immunoelectrophoresis with immunoblotting for detection of anti-extractable nuclear antigen ... Anti-extractable nuclear antigen (ENA) antibodies were assayed by counter immunoelectrophoresis (CIE) and immunoblotting in ...
The full text of research papers can be submitted to the repository via Pure, the Universitys research information system. For help see our guide: How to deposit in Pure.. ...
Shivananda PG, Sarvamangala Devi JN, Rao PV, Baby H. Counterimmunoelectrophoresis in respiratory candidiasis. Indian Journal of ...
Double immunodiffusion (DID) and counterimmunoelectrophoresis (CIEP), two forms of gel-based techniques, are used to gain ... Bunn, C.; Kveder, T. (1996). "Counterimmunoelectrophoresis and immunodiffusion for the detection of antibodies to soluble ... and counterimmunoelectrophoresis (CIEP). Both of these techniques require the precipitation of antigens for valid results. ...
Uses of Counterimmunoelectrophoresis. Counterimmunoelectrophoresis is used to detect presence of capsular antigens in CSF of ... Counterimmunoelectrophoresis (CIE): Principle, Procedure and Uses. Counterimmunoelectrophoresis (CIE): Principle, Procedure and ... Counterimmunoelectrophoresis (CIE) is a modification of Ouchterlony method that speeds up migration of an antigen and antibody ...
Sputum counterimmunoelectrophoresis in the diagnosis of pneumococcal pneumonia. The American Review of Respiratory Disease ... Coagglutination and counterimmunoelectrophoresis for detection of pneumococcal antigens in the sputum of pneumonia patients. ( ... Detection of pneumococcal polysaccharide in the sputum of patients with pneumococcal pneumonia by counterimmunoelectrophoresis ... 12 had pneumococcal antigens detected in sputum by counterimmunoelectrophoresis (CIE), 13 had a positive sputum culture, and 12 ...
Among the several modes of diagnostic testing for infections, serological (i.e. antibody determination) studies are still heavily used. Serodiagnosis of the Infectious Diseases examines this topic in
counterimmunoelectrophoresis. 26-letter words that end in ectrophoresis. *radioimmunoelectrophoresis. 22-letter words that end ...
The counterimmunoelectrophoresis test (CIE) was developed for the diagnosis of C. fetus infection in cattle. It was positive in ... Development and application of the counterimmunoelectrophoresis test for the immunodiagnosis of C. fetus infection in cattle.. ...
... by counterimmunoelectrophoresis) and anti-dsDNA (by enzyme-immunoassay) in the sera of 488 consecutive autoimmune patients. It ...
HI, CF, NT, IFA, counter immunoelectrophoresis Footnotes. ,,,Click on the PDF icon to the left to view a copy of this virus ...
Comparison of counterimmunoelectrophoresis and complement fixation. Author(s): *Bueno, J. P. ... counterimmunoelectrophoresis (CIE) and complement fixation. Two control groups composed of 19 healthy individuals and 12 ...
Serological diagnosis of typhoid fever by counterimmunoelectrophoresis. Br Med J (Clin Res Ed) 1981; 282 :1505 (Published 09 ...
counterimmunoelectrophoresis and double immunodiffusion. Maini RN: The consensus workshops for the detection of auto antibodies ...
Serodiagnosis of giardiasis by counterimmunoelectrophoresis and indirect immunofluorescence tests.. Abdel Hamid MY, Makled KM, ...
Counterimmunoelectrophoresis. Fluorescent-Antibody Assays. Poloymerase Chain Reactions. c. Drugs and Vaccines. 16. ...
Counterimmunoelectrophoresis assay for detection of adenovirus antigen. S D Mankikar, P J Middleton, M Petric ... Detection of adenovirus antibody by counterimmunoelectrophoresis. M Petric, P J Middleton, S D Mankikar ...
Comparison with counterimmunoelectrophoresis and erythro-immunoassay. Mycopathologia81:31-37.. OpenUrl. *6.↵. Camargo, Z. P., J ...
CIE, counterimmunoelectrophoresis. *CT, computed tomography. *EG, Echinococcus granulosus. *ELISA, enzyme-linked immunosorbent ...
Antiri-bonucleoprotein antibodies in connective tissue diseases: examination by counterimmunoelectrophoresis. Br J Med. 1977;1: ...
  • Counterimmunoelectrophoresis is a laboratory technique used to evaluate the binding of an antibody to its antigen, it is similar to immunodiffusion, but with the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel. (wikipedia.org)
  • Less satisfactory results were obtained by counterimmunoelectrophoresis when two other preparations, a protein extract (Barber antigen) and an ultrasonic lysate, were used as the antigens. (bmj.com)
  • It is concluded that in an area where typhoid is endemic counterimmunoelectrophoresis using veronal buffer extract as the antigen is superior to the Widal test for serological diagnosis of typhoid fever. (bmj.com)
  • We used counterimmunoelectrophoresis (CIEP) with rabbit antibodies to Dirofilaria immitis and Brugia malayi to detect soluble filarial antigen in sera collected in a Wuchereria bancrofti -endemic area in South India. (ajtmh.org)
  • Counterimmunoelectrophoresis was performed on a cellulose acetate membrane with dilutions of a commercially available antigen which interacts with sera of patients with confirmed hydatid disease to produce the arc 5 precipitin line. (ajtmh.org)
  • Counterimmunoelectrophoresis (CIE) is a modification of Ouchterlony method that speeds up migration of an antigen and antibody by applying an electrical current. (microbeonline.com)
  • Counterimmunoelectrophoresis assay for detection of adenovirus antigen. (asm.org)
  • Counterimmunoelectrophoresis (CIE) is a procedure in which oppositely charged antigen and antibody are propelled toward each other by an electrical field. (specialtylabs.com)
  • Although pneumococcal polysaccharide antigen was found by counterimmunoelectrophoresis in 25 of 30 middle ear effusions, it could not be detected in the serum from infected animals. (umn.edu)
  • By immunoelectrophoresis or counterimmunoelectrophoresis, type III antigen failed to react with group-specific antiserum and the group B antigen failed to react with type III antiserum. (asm.org)
  • Methods: Sera of 625 consecutive patients with either limited cutaneous or diffuse cutaneous SSc were tested for antibodies to Ku antigen together with other extractable nuclear antigens by counterimmunoelectrophoresis. (elsevier.com)
  • Resumen en inglés A latex reagent and an antiserum for counterimmunoelectrophoresis (CIE)and capsular swelling (CS), both produced by the Acute Bacterial Respiratory Infections Lab of "Pedro Kourí" Institute were tested in the detection of a Haemophilus influenzae type B- specific capsular antigen. (worldwidescience.org)
  • Counterimmunoelectrophoresis and immunoblotting were used to test for antibodies to extractable nuclear antigens. (nih.gov)
  • Of 14 patients with definite or probable pneumococcal pneumonia, 12 had pneumococcal antigens detected in sputum by counterimmunoelectrophoresis (CIE), 13 had a positive sputum culture, and 12 had a Gram-stained smear of sputum suggestive of the diagnosis. (meta.org)
  • Sera were tested for the presence of antibodies to extractable nuclear antigens (anti-ENA) by counterimmunoelectrophoresis, enzyme-linked and immunoblot (IB) assay, and for the presence of anti-centromere antibodies (ACA) by indirect immunofluorescence on HEp-2 cells and IB. (mendeley.com)
  • The complex antigenic properties of adenovirus-soluble complete and incomplete hemagglutinins were utilized to study the specificity of adenovirus anti-hemagglutinin sera in immunodiffusion and counterimmunoelectrophoresis tests. (jimmunol.org)
  • Double immunodiffusion (DID) and counterimmunoelectrophoresis (CIEP), two forms of gel-based techniques, are used to gain information on the clinical significance and the role of these antibodies in those with CTDs. (wikipedia.org)
  • Two widely used gel-based techniques were used to identify anti-ENAs and their associations to disease in early work, double immunodiffusion (DID) [1] and counterimmunoelectrophoresis (CIEP). (wikipedia.org)
  • Immuno Concepts compared the results to reference methods such as Ouchterlony immunodiffusion, counterimmunoelectrophoresis, other ELISA methods and Western blotting. (bio-medicine.org)
  • Detection of adenovirus antibody by counterimmunoelectrophoresis. (asm.org)
  • Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
  • RÉSUMÉ L'objectif de cette étude réalisée en Iraq était de définir la sensibilité et la spécificité d'un test ELISA commercial pour la détection de l'antigène de Giardia lamblia dans les selles. (who.int)
  • Assesment of validity of counterimmunoelectrophoresis and ELISA in the routine diagnosis of amoebiasis. (fcorel.ru)
  • For this purpose, we retrospectively analysed the results of tests for FANA (on Hep-2 cells, positive FANA titers greater than or equal to 1:80), anti-ENA (antibodies to Ro (SSA), La(SSB), U1 nRNP and Sm, by counterimmunoelectrophoresis) and anti-dsDNA (by enzyme-immunoassay) in the sera of 488 consecutive autoimmune patients. (nih.gov)
  • An additional advantage of counterimmunoelectrophoresis is that results may be obtained on the same day that the serum specimens are received. (bmj.com)
  • Serum from male rabbits was assayed for precipitins against Aspergillus (A.) terreus by counterimmunoelectrophoresis. (cdc.gov)
  • Serological diagnosis of typhoid fever by counterimmunoelectrophoresis. (bmj.com)
  • The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella typhi were compared with those of the Widal test in the serological diagnosis of typhoid fever. (bmj.com)
  • Tsang R S , Chau P Y . Serological diagnosis of typhoid fever by counterimmunoelectrophoresis. (bmj.com)
  • A modified counterimmunoelectrophoresis and a conventional indirect hemagglutination test were compared for routine diagnosis of human hydatid disease in an endemic area in the Middle East. (ajtmh.org)
  • The counterimmunoelectrophoresis test (CIE) was developed for the diagnosis of C. fetus infection in cattle. (ac.ke)
  • Latex agglutination (LA) was compared with counterimmunoelectrophoresis (CIE) for the diagnosis of pneumonia due to Streptococcus pneumoniae or Haemophilus influenzae type b in children less than 6 years old in Papua New Guinea. (eurekamag.com)
  • Circulating antibodies to P. brasiliensis can be detected in the patients' sera by serological assays such as DI, counterimmunoelectrophoresis and immunoenzymatic assays (6, 20). (scielo.br)
  • Cross-reactivity with sera of patients with other parasitic infections was noted with indirect hemagglutination but not with counterimmunoelectrophoresis. (ajtmh.org)
  • Counterimmunoelectrophoresis was particularly well suited to a rapid identification and typing of adenovirus isolates for diagnostic purposes. (jimmunol.org)
  • Antibodies against P. brasiliensis were detected by indirect immunofluorescence (IF) (IgG, IgM and IgA isotypes), counterimmunoelectrophoresis (CIE) and complement fixation. (unesp.br)
  • Both are more sensitive than conventional counterimmunoelectrophoresis. (we-rock.cc)
  • Sensitivity (95.5% vs. 93.2%) and specificity (99.2% vs. 89.9%) were higher with counterimmunoelectrophoresis than with indirect hemagglutination. (ajtmh.org)
  • Development and application of the counterimmunoelectrophoresis test for the immunodiagnosis of C. fetus infection in cattle. (ac.ke)
  • Nous recommandons l'utilisation de la méthode immunoenzymatique dans les études épidémiologiques en Iraq, ainsi que pour confirmer le diagnostic chez les patients présentant des symptômes cliniques caractéristiques de la giardiase mais dont les résultats par microscopie directe sont négatifs. (who.int)
  • Melar Immunoprecipitation Chromatin immunoprecipitation Immunodiffusion Ouchterlony double immunodiffusion Radial immunodiffusion Immunoelectrophoresis Counterimmunoelectrophoresis. (baustyle.ru)