Coumestrol: A daidzein derivative occurring naturally in forage crops which has some estrogenic activity.Phytoestrogens: PLANT EXTRACTS and compounds, primarily ISOFLAVONES, that mimic or modulate endogenous estrogens, usually by binding to ESTROGEN RECEPTORS.Estrogens, Non-Steroidal: Non-steroidal compounds with estrogenic activity.Lignans: A class of dibenzylbutane derivatives which occurs in higher plants and in fluids (bile, serum, urine, etc.) in man and other animals. These compounds, which have a potential anti-cancer role, can be synthesized in vitro by human fecal flora. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Isoflavones: 3-Phenylchromones. Isomeric form of FLAVONOIDS in which the benzene group is attached to the 3 position of the benzopyran ring instead of the 2 position.Plant Root Nodulation: The formation of a nitrogen-fixing cell mass on PLANT ROOTS following symbiotic infection by nitrogen-fixing bacteria such as RHIZOBIUM or FRANKIA.Estrogens: Compounds that interact with ESTROGEN RECEPTORS in target tissues to bring about the effects similar to those of ESTRADIOL. Estrogens stimulate the female reproductive organs, and the development of secondary female SEX CHARACTERISTICS. Estrogenic chemicals include natural, synthetic, steroidal, or non-steroidal compounds.Genitalia, Female: The female reproductive organs. The external organs include the VULVA; BARTHOLIN'S GLANDS; and CLITORIS. The internal organs include the VAGINA; UTERUS; OVARY; and FALLOPIAN TUBES.Estradiol Congeners: Steroidal compounds related to ESTRADIOL, the major mammalian female sex hormone. Estradiol congeners include important estradiol precursors in the biosynthetic pathways, metabolites, derivatives, and synthetic steroids with estrogenic activities.Plant Preparations: Material prepared from plants.Equipment Safety: Freedom of equipment from actual or potential hazards.Device Approval: Process that is gone through in order for a device to receive approval by a government regulatory agency. This includes any required preclinical or clinical testing, review, submission, and evaluation of the applications and test results, and post-marketing surveillance. It is not restricted to FDA.United States Food and Drug Administration: An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.Therapeutic Equivalency: The relative equivalency in the efficacy of different modes of treatment of a disease, most often used to compare the efficacy of different pharmaceuticals to treat a given disease.Kidney Transplantation: The transference of a kidney from one human or animal to another.Hematopoietic Stem Cell Mobilization: The release of stem cells from the bone marrow into the peripheral blood circulation for the purpose of leukapheresis, prior to stem cell transplantation. Hematopoietic growth factors or chemotherapeutic agents often are used to stimulate the mobilization.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Diet, Reducing: A diet designed to cause an individual to lose weight.Weight Loss: Decrease in existing BODY WEIGHT.Arecaceae: The palm family of order Arecales, subclass Arecidae, class Liliopsida.History, 20th Century: Time period from 1901 through 2000 of the common era.Diet: Regular course of eating and drinking adopted by a person or animal.Obesity: A status with BODY WEIGHT that is grossly above the acceptable or desirable weight, usually due to accumulation of excess FATS in the body. The standards may vary with age, sex, genetic or cultural background. In the BODY MASS INDEX, a BMI greater than 30.0 kg/m2 is considered obese, and a BMI greater than 40.0 kg/m2 is considered morbidly obese (MORBID OBESITY).Physician Incentive Plans: Compensatory plans designed to motivate physicians in relation to patient referral, physician recruitment, and efficient use of the health facility.Soybeans: An annual legume. The SEEDS of this plant are edible and used to produce a variety of SOY FOODS.Butylene Glycols: 4-carbon straight chain aliphatic hydrocarbons substituted with two hydroxyl groups. The hydroxyl groups cannot be on the same carbon atom.Soybean Proteins: Proteins which are present in or isolated from SOYBEANS.Medicago sativa: A plant species of the family FABACEAE widely cultivated for ANIMAL FEED.Genistein: An isoflavonoid derived from soy products. It inhibits PROTEIN-TYROSINE KINASE and topoisomerase-II (DNA TOPOISOMERASES, TYPE II); activity and is used as an antineoplastic and antitumor agent. Experimentally, it has been shown to induce G2 PHASE arrest in human and murine cell lines and inhibits PROTEIN-TYROSINE KINASE.Tuna: Common name for various species of large, vigorous ocean fishes in the family Scombridae.Adipogenesis: The differentiation of pre-adipocytes into mature ADIPOCYTES.Rhus: A plant genus of the family Anacardiaceae, order Sapindales, subclass Rosidae. It is a source of gallotannin (TANNIC ACID) and of somewhat edible fruit. Do not confuse with TOXICODENDRON which used to be part of this genus.3T3-L1 Cells: A continuous cell line that is a substrain of SWISS 3T3 CELLS developed though clonal isolation. The mouse fibroblast cells undergo an adipose-like conversion as they move to a confluent and contact-inhibited state.Molecular Mechanisms of Pharmacological Action: Pharmacological activities at the molecular level of DRUGS and other exogenous compounds that are used to treat DISEASES and affect normal BIOCHEMISTRY.Angiotensin Amide: The octapeptide amide of bovine angiotensin II used to increase blood pressure by vasoconstriction.Click Chemistry: Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.Sesquiterpenes, Eudesmane: SESQUITERPENES cyclized into two adjoining cyclohexane rings but with a different configuration from the ARTEMISININS.Endocrinology: A subspecialty of internal medicine concerned with the metabolism, physiology, and disorders of the ENDOCRINE SYSTEM.Practice Guidelines as Topic: Directions or principles presenting current or future rules of policy for assisting health care practitioners in patient care decisions regarding diagnosis, therapy, or related clinical circumstances. The guidelines may be developed by government agencies at any level, institutions, professional societies, governing boards, or by the convening of expert panels. The guidelines form a basis for the evaluation of all aspects of health care and delivery.United StatesSocieties, Medical: Societies whose membership is limited to physicians.Menopause: The last menstrual period. Permanent cessation of menses (MENSTRUATION) is usually defined after 6 to 12 months of AMENORRHEA in a woman over 45 years of age. In the United States, menopause generally occurs in women between 48 and 55 years of age.Clinical Trials as Topic: Works about pre-planned studies of the safety, efficacy, or optimum dosage schedule (if appropriate) of one or more diagnostic, therapeutic, or prophylactic drugs, devices, or techniques selected according to predetermined criteria of eligibility and observed for predefined evidence of favorable and unfavorable effects. This concept includes clinical trials conducted both in the U.S. and in other countries.Societies, Nursing: Societies whose membership is limited to nurses.Talin: A 235-kDa cytoplasmic protein that is also found in platelets. It has been localized to regions of cell-substrate adhesion. It binds to INTEGRINS; VINCULIN; and ACTINS and appears to participate in generating a transmembrane connection between the extracellular matrix and the cytoskeleton.Long QT Syndrome: A condition that is characterized by episodes of fainting (SYNCOPE) and varying degree of ventricular arrhythmia as indicated by the prolonged QT interval. The inherited forms are caused by mutation of genes encoding cardiac ion channel proteins. The two major forms are ROMANO-WARD SYNDROME and JERVELL-LANGE NIELSEN SYNDROME.1,2-Dimethylhydrazine: A DNA alkylating agent that has been shown to be a potent carcinogen and is widely used to induce colon tumors in experimental animals.Genome: The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Dimethylhydrazines: Hydrazines substituted with two methyl groups in any position.Vinculin: A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10.

Induction of hyperplasia and increased DNA content in the uterus of immature rats exposed to coumestrol. (1/37)

Administration of the phytoestrogen coumestrol to ovariectomized rats leads to increases in both wet and dry uterine weights in the absence of an increase in uterine DNA content, as reported by Markaverich et al. [Effects of Coumestrol on Estrogen Receptor Function and Uterine Growth in Ovariectomized Rats. Environ Health Perspect 103:574-581 (1995)]. It was not possible to know if the observed atypical uterotrophic response of coumestrol was associated uniquely with the ovariectomized uterotrophic assay protocol. This question is answered in the present paper. Two experiments are described in which three daily oral gavage administrations of 60 mg/kg/day coumestrol to immature AP rats were followed by assessment of the reproductive tract on the fourth day. In both experiments coumestrol increased uterine fluid content and increased the weights of the uterus, cervix, and vagina. In addition, bromodeoxyuridine staining of uterine sections enabled confirmation of uterine hyperplasia for the coumestrol-treated animals. In the second experiment, total uterine DNA was determined; it doubled in the coumestrol-treated animals. Estradiol benzoate acted as the positive control agent for both of these experiments, and in each case it gave similar responses to those seen for coumestrol. We conclude that the uterotrophic activity of the phytoestrogen coumestrol in the immature intact rat is typical of the activity of the natural estrogen estradiol.  (+info)

Evaluation of a Tier I screening battery for detecting endocrine-active compounds (EACs) using the positive controls testosterone, coumestrol, progesterone, and RU486. (2/37)

After previously examining 12 compounds with known endocrine activities, we have now evaluated 4 additional compounds in a Tier I screening battery for detecting endocrine-active compounds (EACs): a weak estrogen receptor (ER) agonist (coumestrol; COUM), an androgen receptor (AR) agonist (testosterone; TEST), a progesterone receptor (PR) agonist (progesterone; PROG), and a PR antagonist (mifepristone; RU486). The Tier I battery incorporates 2 short-term in vivo tests (5-day ovariectomized female battery; 15-day intact male battery) and an in vitro yeast transactivation system (YTS). The Tier I battery is designed to identify compounds that have the potential to act as agonists or antagonists to the estrogen, androgen, progesterone, or dopamine receptors; steroid biosynthesis inhibitors (aromatase, 5alpha-reductase, and testosterone biosynthesis); or compounds that alter thyroid function. In addition to the Tier I battery, a 15-day dietary restriction experiment was performed using male rats to assess confounding due to treatment-related decreases in body weight. In the Tier I female battery, TEST administration increased uterine weight, uterine stromal cell proliferation, and altered hormonal concentrations (increased serum testosterone [T] and prolactin [PRL]; and decreased serum FSH and LH). In the male battery, TEST increased accessory sex gland weights, altered hormonal concentrations (increased serum T, dihydrotestosterone [DHT], estradiol [E2], and PRL; decreased serum FSH and LH), and produced microscopic changes of the testis (Leydig cell atrophy and spermatid retention). In the YTS, TEST activated gene transcription in the yeast containing the AR or PR. In the female battery, COUM administration increased uterine weight, uterine stromal cell proliferation, and uterine epithelial cell height, and increased serum PRL concentrations. In the male battery, COUM altered hormonal concentrations (decreased serum T, DHT, E2; increased serum PRL) and, in the YTS, COUM activated gene transcription in the yeast containing the ER. In the female battery, PROG administration increased uterine weight, uterine stromal cell proliferation, and uterine epithelial cell height and altered hormonal concentrations (increased serum progesterone and decreased serum FSH and LH). In the male battery, PROG decreased epididymis and accessory sex gland weights, altered hormonal concentrations (decreased serum T, PRL, FSH, and LH; increased serum progesterone and E2), and produced microscopic changes of the testis (Leydig cell atrophy). In the YTS, PROG activated gene transcription in the yeast containing the AR or PR. In the female battery, RU486 administration increased uterine weight and decreased uterine stromal cell proliferation. In the male battery, RU486 decreased epididymis and accessory sex gland weights and increased serum FSH and LH concentrations. In the YTS, RU486 activated gene transcription in the yeast containing the ER, AR, or PR. Dietary restriction data demonstrate that confounding due to decrements in body weight are not observed when body weight decrements are 10% or less in the Tier I male battery. In addition, minimal confounding is observed at body decrements of 15% (relative liver weight, T3, and T4). Hence, compounds can be evaluated in this Tier I at levels that produce a 10% decrease in body weight without confounding of the selected endpoints. Using the responses obtained for all the endpoints in the Tier I battery, a distinct "fingerprint" was produced for each type of endocrine activity against which compounds with unknown activity can be compared. These data demonstrate that the described Tier I battery is useful for identifying EACs and they extend the compounds evaluated to 16.  (+info)

Estradiol-type activity of coumestrol in mature and immature ovariectomized rat uterotrophic assays. (3/37)

Makaverich et al. [Environ Health Perspect 103:574-581 (1995)] reported that the uterotrophic activity of the phytoestrogen coumestrol in the immature ovariectomized rat was atypical in that it was not associated with increased uterine hyperplasia and DNA content. We previously reported that coumestrol gave a typical estradiol-type uterotrophic response in the immature intact rat, yielding increases in uterine epithelial cell height, glandular formation, cell labeling, and DNA content. These papers did not answer the question of whether there is a basic difference between the ovariectomized and the intact rat uterotrophic assays. In this paper, we report that coumestrol gives a typical estradiol-type uterotrophic response in uterotrophic assays using immature intact, immature ovariectomized, and mature ovariectomized rats. We concluded that the uterotrophic activity of coumestrol is typical of the natural estrogen estradiol.  (+info)

Potential estrogenic and antiestrogenic activity of the cyclic siloxane octamethylcyclotetrasiloxane (D4) and the linear siloxane hexamethyldisiloxane (HMDS) in immature rats using the uterotrophic assay. (4/37)

The cyclic siloxane octamethylcyclotetrasiloxane (D4) and the linear siloxane hexamethyldisiloxane (HMDS) have numerous industrial and consumer applications and thus have the potential for human exposure. The present study was undertaken to examine potential estrogenic and antiestrogenic activities of D4 and HMDS. To address potential differences in sensitivity between rat strains the study used both Sprague-Dawley (SD) and Fischer 344 (F-344) rats. Estrogenicity of the test compounds was determined by measuring absolute and relative uterine weights in immature rats and by monitoring uterine epithelial cell height. In order to place the data obtained for D4 into perspective relative to strong and weak estrogenic compounds, the response produced by D4 at 0, 10, 50, 100, 250, 500, and 1000 mg/kg/day was compared to responses produced by ethinyl estradiol (EE) (1, 3, 10, or 30 microg/kg/day), diethylstilbestrol dipropionate (DES-DP) (0.5, 1.5, 5, 15 microg/kg/day), and coumestrol (CE) (10, 35, 75, 150 mg/kg/day). Antiestrogenic effects were evaluated by co-administering D4 (500 mg/kg/day) with EE at 1, 3, 10, and 30 microg /kg/day. All compounds were administered in sesame oil at a volume of 5 mL/kg by oral gavage. Beginning on postnatal day 18 (SD) or 21 (F-344) each pup (12 per group) received a single dose of test compound once a day for 4 consecutive days. The pups were euthanized the morning after the last treatment and their uteri removed, weighed, and processed for histological examination. EE and DES-DP produced a significant dose-dependent increase in absolute and relative uterine weights and uterine cell height. The maximum increase in uterine weight following EE exposure was approximately 350% relative to controls in both strains. The weak phytoestrogen CE also produced a dose-related increase in absolute and relative uterine weight and epithelial cell height, but the response occurred over a much higher range of doses. At the highest dose of CE, uterine weight was increased approximately 230% relative to controls. Following exposure to D4, absolute and relative uterine weights and uterine epithelial cell height were statistically significantly increased in both strains of rats at doses above 100 mg/kg/day. In terms of uterine weight, D4 was approximately 0.6 million times less potent than EE or DES-DP in SD pups and 3.8 million times less potent than EE or DES-DP in F-344 pups. The maximal increase in uterine weight, relative to controls, produced by D4 at 1000 mg/kg/day was approximately 160% in SD rats, while the maximum increase produced by D4 in F-344 rats was 86%. D4 co-administered over a wide range of EE doses, resulted in a significant reduction in uterine weight compared to EE alone. HMDS was evaluated in SD rats only. The response produced by HMDS (600 and 1200 mg/kg/day) was compared to EE (3 microg/kg/day). Antiestrogenic effects were evaluated by co-administering HMDS (1200 mg/kg/day) with EE at 3 microg/kg/day. HMDS had no measurable effect on uterine weight under the experimental conditions described here. However, HMDS coadministered with EE did produce a small, but statistically significant reduction in uterine weight compared to EE alone. In conclusion, D4 showed weak estrogenic and antiestrogenic activity that was several orders of magnitude less potent than EE, and many times less potent than the weak phytoestrogen CE.  (+info)

Prenatal exposure to estrogenic compounds alters the expression pattern of platelet-derived growth factor receptors alpha and beta in neonatal rat testis: identification of gonocytes as targets of estrogen exposure. (5/37)

We examined the effects of maternal exposure to estrogens on platelet-derived growth factor (PDGF) receptor (PDGFR) expression in newborn rat testis. Pregnant rats were treated from gestation Day 14 to birth with corn oil containing diethylstilbestrol, bisphenol A, genistein, or coumestrol by gavage or subcutaneous injection. These treatments induced a dose-dependent increase in the expression of PDGFR alpha and beta mRNAs, determined by semiquantitative reverse transcription polymerase chain reaction, though diethylstilbestrol had a biphasic effect on both mRNAs. In situ hybridization analysis showed that PDGFRalpha mRNA increased mostly in the interstitium, while PDGFRbeta mRNA increased both in the interstitium and seminiferous cords. Immunohistochemical studies of PDGFRalpha and beta proteins revealed that both receptors were present in testis before and after birth and that they were upregulated upon treatment with estrogens in 3-day-old rats, with PDGFRbeta increasing dramatically in gonocytes. PDGFRalpha and beta mRNAs and proteins were also found in purified gonocytes. Our previous finding that PDGF and 17beta-estradiol induce gonocyte proliferation in vitro, together with the present finding that in vivo exposure to estrogens upregulates PDGF receptors in testis, suggest that PDGF pathway is a target of estrogens in testis. In addition, these data identify PDGFRbeta in gonocytes as a major target of gestational estrogen exposure, suggesting that estrogen may have a physiological interaction with PDGF during gonocyte development. These results, however, do not exclude the possibility that the effects of the compounds examined in this study might be due to estrogen receptor-independent action(s).  (+info)

The OECD program to validate the rat uterotrophic bioassay. Phase 2: dietary phytoestrogen analyses. (6/37)

Many commercial laboratory diets have detectable levels of isoflavones (e.g., phytoestrogens such as genistein [GN]) that have weak estrogenic activity both in vitro and in vivo. During validation studies of the uterotrophic bioassay, diet samples from 20 participating laboratories were collected and analyzed for three major phytoestrogens: GN, daidzein (DN), and coumestrol (CM). Soy phytoestrogens GN and DN were found at total phytoestrogen levels from 100 to 540 microg/g laboratory diet; a forage phytoestrogen, CM, ranged from nondetectable to 4 microg/g laboratory diet. The phytoestrogen levels were compared with both baseline uterine weights of the control groups and with the relative uterine weight increase of groups administered two weak estrogen agonists: bisphenol A (BPA) and nonylphenol (NP). The comparison uses a working assumption of additivity among the phytoestrogens, despite several significant qualifications to this assumption, to estimate total genistein equivalents (TGE). Some evidence was found that phytoestrogen levels in the diet > 325-350 microg/g TGE could diminish the responsiveness of the uterotrophic bioassay to weak agonists. This was especially true for the case of the intact, immature female version of the uterotrophic bioassay, where higher food consumption relative to body weight leads to higher intakes of dietary phytoestrogens versus ovariectomized adults. This dietary level is sufficient in the immature female to approach a biological lowest observable effect level for GN of 40-50 mg/kg/day. These same data, however, show that low to moderate levels of dietary phytoestrogens do not substantially affect the responsiveness of the assay with weak estrogen receptor agonists such as NP and BPA. Therefore, laboratories conducting the uterotrophic bioassay for either research or regulatory purposes may routinely use diets containing levels of phytoestrogens < 325-350 microg/g TGE without impairing the responsiveness of the bioassay.  (+info)

Modulation of tumor formation and intestinal cell migration by estrogens in the Apc(Min/+) mouse model of colorectal cancer. (7/37)

Epidemiological studies suggest that post-menopausal hormone replacement therapy (HRT) reduces colorectal cancer (CRC) incidence. Phytoestrogens, including the soy isoflavone genistein and coumestrol, are used by many women as alternatives to HRT. Previous studies showed that ovariectomy induced a 77% increase in intestinal adenoma number in the C57BL/6J-Min/+ (Min/+) mouse, an animal model of adenomatous polyposis coli (APC)-associated CRC. Replacement of estradiol (E(2)) in ovariectomized Min/+ mice reduced tumor number to baseline and up-regulated the expression of estrogen receptor beta (ERbeta). We hypothesized that the phytoestrogens genistein and coumestrol would inhibit intestinal tumorigenesis in ovariectomized Min/+ mice. Min/+ and Apc(+/+) (WT) mice were ovariectomized and assigned to either a control diet or treatment with E(2), genistein or coumestrol. Treatment of ovariectomized Min/+ (Min/+ OX) mice with genistein resulted in a non-significant reduction in tumor number. Min/+ OX mice treated with coumestrol had significantly fewer tumors than untreated Min/+ OX controls and the same number of tumors as non-ovariectomized Min/+ mice. Bromodeoxyuridine migration assays also demonstrated that treatment with E(2) or coumestrol improved enterocyte migration rate. Immunoprecipitation and immunohistochemistry analyses showed that impaired association of the adherens junction proteins E-cadherin and beta-catenin in Min/+ mice was improved by treatment with either E(2) or coumestrol. Immunoblot analyses also showed that expression of ERbeta was elevated in enterocytes of Min/+ OX mice treated with E(2) or coumestrol as compared with those of untreated Min/+ OX mice. In conclusion, both coumestrol and E(2) prevent intestinal tumorigenesis and ameliorate enterocyte migration and intercellular adhesion in the Apc(Min/+) mouse model of CRC.  (+info)

Xenoestrogens at picomolar to nanomolar concentrations trigger membrane estrogen receptor-alpha-mediated Ca2+ fluxes and prolactin release in GH3/B6 pituitary tumor cells. (8/37)

Xenoestrogens (XEs) are widespread in our environment and are known to have deleterious effects in animal (and perhaps human) populations. Acting as inappropriate estrogens, XEs are thought to interfere with endogenous estrogens such as estradiol (E2) to disrupt normal estrogenic signaling. We investigated the effects of E2 versus several XEs representing organochlorine pesticides (dieldrin, endosulfan, o',p'-dichlorodiphenylethylene), plastics manufacturing by-products/detergents (nonylphenol, bisphenol A), a phytoestrogen (coumestrol), and a synthetic estrogen (diethylstilbestrol) on the pituitary tumor cell subline GH3/B6/F10, previously selected for expression of high levels of membrane estrogen receptor-alpha. Picomolar to nanomolar concentrations of both E2 and XEs caused intracellular Ca2+ changes within 30 sec of administration. Each XE produced a unique temporal pattern of Ca2+ elevation. Removing Ca2+ from the extracellular solution abolished both spontaneous and XE-induced intracellular Ca2+ changes, as did 10 microM nifedipine. This suggests that XEs mediate their actions via voltage-dependent L-type Ca2+ channels in the plasma membrane. None of the Ca2+ fluxes came from intracellular Ca2+ stores. E2 and each XE also caused unique time- and concentration-dependent patterns of prolactin (PRL) secretion that were largely complete within 3 min of administration. PRL secretion was also blocked by nifedipine, demonstrating a correlation between Ca2+ influx and PRL secretion. These data indicate that at very low concentrations, XEs mediate membrane-initiated intracellular CCa2+ increases resulting in PRL secretion via a mechanism similar to that for E2, but with distinct patterns and potencies that could explain their abilities to disrupt endocrine functions.  (+info)

Ovariectomized rats were pretreated with intravenous estradiol-17 beta (E2), coumestrol or genistein and were challenged 2 h later with GnRH (50 ng/kg BW, i.v.). Blood samples, drawn at the time of GnRH administration and 15 min afterwards, were assayed for luteinizing hormone (LH). While low-dose E2 pretreatment (10 ng/kg BW) significantly enhanced GnRH-induced LH release,
We recently showed that two different ALDH+ and CD44+/CD24-/low breast cancer stem cells (BSCSs) exhibited stem cell characteristics that include self-renewal, extensive proliferation, the ability to form non-adherent spherical clusters, chemotherapy resistance and high Notch1 expression. We have identified a compound compound: 6-(3-methylbut-2-enyl) coumestrol (Pso) and treatment with Pso resulted in growth inhibition and an EMT phenotype in both BCSCs and BC cells. Oral Pso administration at physiologically achievable doses (25 mg/kg/BW) suppressed the growth of BCSCs and BC xenografts without toxicity. In the current studies, we identified several novel Pso-derived analogs that may be more potent than the parent compound. One such compound, 1-methoxyphaseollidin (1MP), obtained via three main functional group changes: (i) translocation of the isoprenyl moiety from the phenyl ring fused to the pyran ring (as in Pso) to the phenyl ring adjacent to the furan ring, (ii) removal of the carbonyl ...
We are all fans of greens blended in smoothies, but they are also excellent when blended for a soup! This is a very thick but nourishing and light soup that combines so many nutrient-dense greens! Peas are high in fiber at 9g per cup and protein at 8g per cup which makes it a great food for healthy weight control. This soup is full of chlorophyll and rich with important micronutrients such as Vitamin K, manganese, iron, calcium, Vitamin B and Vitamin C. Peas are also high in antioxidants, one called coumestrol that has been shown to protect against stomach cancer ...
Sigma-Aldrich offers abstracts and full-text articles by [Chris Talsness, Konstanze Grote, Sergio Kuriyama, Kenia Presibella, Anja Sterner-Kock, Katia Poça, Ibrahim Chahoud].
Aims: Development of urinary incontinence, for many women, occurs following menopause. Dietary phytoestrogens consumed over the long term may affect the contractile function and maintenance of the urinary bladder in post menopausal women. This study examined the muscarinic receptor mediated contractile responses in the rat isolated bladder in response to ovariectomy and long term dietary phytoestrogen consumption. Main methods: Ovariectomised or sham-operated female Wistar rats (8 weeks) were fed either normal rat chow (soy, phytoestrogens) or a non-soy (phytoestrogen free) diet. Bladders were dissected from rats at 12, 24 and 52 weeks of age and placed in 25 ml organ baths filled with McEwans solution. Key findings: The contractile response to carbachol, in 12 week old female rats did not change as a result of dietary phytoestrogens or ovariectomy (PN0.05). At 24 weeks of age, detrusor muscle strip responses to carbachol from non-soy fed ovariectomised rats were attenuated (Pb0.05). At 52 ...
Phytoestrogens have been investigated as natural alternatives to hormone replacement therapy and their potential as chemopreventive agents. We investigated the effects of equol, genistein, and coumestrol on cell growth in fully estrogenized MCF7 cells, simulating the perimenopausal state, and long-term estrogen-deprived MCF7:5C cells, which simulate the postmenopausal state of a woman after years of estrogen deprivation, and compared the effects with that of steroidal estrogens: 17β estradiol (E2) and equilin present in conjugated equine estrogen. Steroidal and phytoestrogens induce proliferation of MCF7 cells at physiologic concentrations but inhibit the growth and induce apoptosis of MCF7:5C cells. Although steroidal and phytoestrogens induce estrogen-responsive genes, their antiproliferative and apoptotic effects are mediated through the estrogen receptor. Knockdown of ERα using siRNA blocks all estrogen-induced apoptosis and growth inhibition. Phytoestrogens induce endoplasmic reticulum ...
Dietary phytoestrogens present in soy dramatically increase cardiotoxicity in male mice receiving a chemotherapeutic tyrosine kinase inhibitor Journal Article ...
The new bone health nutrient. Potent bone-enhancing benefits are reported for ipriflavone, a type of isoflavone, that works by a non-hormonal mechanism. By inhibiting breakdown of bone, ipriflavone favorably affects bone density. Available separately or as part of our new Osteo-guard Plus Ipriflavone, a more complete bone support formula. Ipriflavone 300 mg. Suggested Usage: 1 capsule twice daily. Category: Flavonoid Antioxidants
Complete osteoporosis support formula, with ipriflavone; reduces the risk of osteoporosis This broad spectrum combination of minerals, vitamins, and herbs is formulated to aid in the formation and strengthening of the skeletal system. It provides highly bioavailable forms of calcium, a mineral essential not only for the maintenance of the functional integrity of the nervous, muscular, and skeletal systems, but also cell membrane and capillary impermeability. This formula also provides substances which nutritionally support and enhance the absorption of calcium and the incorporation of calcium into bone. In addition, it provides nutrients essential to healthy bone structure and formation, the synthesis and strengthening of connective tissue in cartilage and bone, and the formation of elastin. Ipriflavone, an advanced addition to this formula, is a synthetic derivative of naturally occurring isoflavones. It supports healthy bone maintenance by promoting secretion of the hormone calcitonin, and
Dietary phytoestrogen and fiber intake do not appear to prevent vasomotor symptoms (VMS), including hot flashes and night sweats.
Learn more about Ipriflavone at Sky Ridge Medical Center Uses Principal Proposed Uses Preventing and Treating Osteoporosis ...
Learn more about Ipriflavone at Portsmouth Regional Hospital Uses Principal Proposed Uses Preventing and Treating Osteoporosis ...
Compounds with estrogenic potencies and their adverse effects in surface waters have received much attention. Both anthropogenic and natural compounds contribute to overall estrogenic activity in freshwaters. Recently, estrogenic potencies were also found to be associated with cyanobacteria and their blooms in surface waters. The present study developed and compared the solid phase extraction and LC-MS/MS analytical approaches for determination of phytoestrogens (8 flavonoids - biochanin A, coumestrol, daidzein, equol, formononetin, genistein, naringenin, apigenin - and 5 sterols - ergosterol, β-sitosterol, stigmasterol, campesterol, brassicasterol) and cholesterol in water. The method was used for analyses of samples collected in stagnant water bodies dominated by different cyanobacterial species. Concentrations of individual flavonoids ranged from below the limit of detection to 3.58 ng/L. Sterols were present in higher amounts up to 2.25 μg/L. Biological potencies of these phytoestrogens in vitro
The valuable compounds found in pueraria mirifica, miroestrol and deoxymiroestrol, are different and possess highest estrogenic activity among the known phytoestrogen (natural plant estrogen) plants. It has structural similarity to estradiol of the main human estrogen in women bodies, which can bind the female estrogen receptor at the breast tissues. Other phyto-chemicals are: Isoflavones and groups of phytoestrogens e.g. Genistein, Daidzein, Daizin, Genistin and Coumestrol are usually found in soy plant. The estrogenic activity of miroestrol and deoxymiroestrol (in pueraria mirifica) is much more potent than other soy isoflavones. Isoflavones enriched in pueraria mirifica supports healthy cardio-vascular system, enhances breast and skin appearance, support healthy prostate function and healthy bone structure. Its primarily use is as tonic and preserved feminine longevity; firming and tightening breast, nourishing skin, keeps skin supple, smooth and brilliant, black glittering hair and relieving ...
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Baru Rp 350.000 Royalty Breast up Cream Pembesar Payudara ini diformulasikan khusus untuk memperbesar payudara. Selain itu untuk merawat dan mengencangkan payudara agar padat dan montok. Bagian tubuh ini memiliki peranan penting bagi wanita yaitu untuk menunjang penampilan agar lebih cantik dan seksi. Pembesar Payudara Royalty Breast up Cream yang mengandung bahan alami Ekstrak Pueraria Mirifica. Pueraria Mirifica (juga dikenal sebagai Kwao Krua Putih adalah herbal akar berbonggol alami dan mengandung phytoestrogen alami tingkat tinggi, termasuk miroestrol, deoxymiroestrol, daidzein, genistin, B-sitosterol, stigmasterol, coumestrol, Pueraria, campestrol, mirificoumestan, kwakhurin, dan mirificine. MANFAAT PUERARIA MIRIFICA TERHADAP PAYUDARA : Mengangkat dan mengencangkan kembali payudara dan punggung yang mengendur. Memantapkan kembali lagi bentuk payudara menjadikan lebih padat dan berisi. Memperbesar payudara yang mengalami pertumbuhan yang terhambat. Menjadikan payudara lebih seksi. Mencerahkan
BACKGROUND: The causes of prostate cancer are poorly understood, but genetic factors may be more important than for many other malignancies, and dietary phytoestrogens may be protective. Because phytoestrogens bind tightly to the estrogen receptor-beta, we conducted an epidemiologic investigation of synergistic effects between phytoestrogen intake and estrogen receptor-beta gene polymorphisms. METHODS: We performed a population-based case-control study in Sweden. All participants reported their phytoestrogen intake and donated a blood sample. We identified four haplotype-tagging single nucleotide polymorphisms (htSNPs) and genotyped these htSNPs in 1314 prostate cancer patients and 782 controls. Odds ratios were estimated by multivariate logistic regression. Interactions between phytoestrogen intake and estrogen receptor-beta SNPs on prostate cancer risk were evaluated considering both multiplicative and additive effect scales. RESULTS: We found a significant multiplicative interaction (P = ...
... is a synthetic isoflavone derivative, which has been suggested to be an inhibitor of bone resorption and a stimulator of osteoblast activity in vitro in cell cultures and in vivo in experimental models of osteoporosis. Preliminary studies, mainly performed in Italy and Japan, suggested that ipriflavone (typical dosage 600 mg/day) is able to prevent bone loss, and some data even suggested that ipriflavone may increase bone mass in postmenopausal women. However, reports of lymphocytopenia in women taking ipriflavone generated concerns regarding the safety of this particular compound. In order to investigate the effect of oral ipriflavone in prevention of postmenopausal bone loss and to assess the safety profile of long-term treatment with ipriflavone in postmenopausal osteoporotic women, a prospective, randomized, placebo-controlled 4-year study was conducted in 474 postmenopausal white women with bone mineral densities below the threshold for a diagnosis of osteoporosis, according to ...
Epidemiological and pathological data suggest that thyroid cancer may well be an estrogen-dependent disease. In the study of the relationship between thyroid cancer risk and dietary phytoestrogens, which can have both estrogenic and antiestrogenic properties, using the present data from a multiethnic population-based case-control study of thyroid cancer conducted in the San Francisco Bay Area. Of 817 cases diagnosed between 1995 and 1998 (1992 and 1998 for Asian women), 608 (74%). Of 793 controls identified through random-digit dialing, 558 (70%) indicated that phytoestrogen consumption was assessed via a food-frequency questionnaire and a newly developed nutrient database. The consumption of traditional and nontraditional soy-based foods and alfalfa sprouts were associated with reduced risk of thyroid cancer. Consumption of "western" foods with added soy flour or soy protein did not affect risk. Of the seven specific phytoestrogenic compounds examined, the isoflavones, daidzein and genistein ...
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The purpose of this study was to examine the effects of phytoestrogens on luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Phytoestrogens are found in soybean based foods. The rats in a previous study were fed varying amounts of phytoestrogens, euthanized, and their pituitaries were preserved for further research, which is what was used in this study. In the previous study there was an increase in spermatogenesis for the low and high phytoestrogen diet group compared to the pre-treatment and medium group. The LH and FSH levels in the pituitaries were measured and the results were then analyzed to see if there was a correlation in LH and FSH levels to the phytoestrogen diet groups. A coomassie stain was done to ensure that the differences that were seen between the groups were because of varying amounts of phytoestrogens and not varying amounts of protein being loaded into the electrophoresis gels. There was no difference measured in any of the phytoestrogen diet groups for LHf3 and LH
In the study to review the sources, metabolism, potencies, and clinical effects of phytoestrogens on humans, posted in PubMed, suggested that phytoestrogens are among the dietary factors affording protection against cancer and heart disease in vegetarians. With this epidemiologic and cell line evidence, intervention studies are now an appropriate consideration to assess the clinical effects of phytoestrogens because of the potentially important health benefits associated with the consumption of foods containing these compounds ...
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a)Scope of section; definitions - (1)Service providers. (i) On or after January 1, 2018 for Tier I carriers and April 1, 2018 for service providers other than Tier I carriers, the hearing aid compatibility requirements of this section apply to providers of digital mobile service in the United States to the extent that they offer terrestrial mobile service that enables two-way real-time voice communications among members of the public or a substantial portion of the public, including both interconnected and non-interconnected VoIP services, and such service is provided over frequencies in the 698 MHz to 6 GHz bands. (ii) Prior to January 1, 2018 for Tier I carriers and April 1, 2018 for service providers other than Tier I carriers, the hearing aid compatibility requirements of this section apply to providers of digital CMRS in the United States to the extent that they offer real-time, two-way switched voice or data service that is interconnected with the public switched network and utilizes an ...
Phytoestrogens occupy estrogen receptor sites, just as a key blank will plug into a lock and not start your car, phytoestrogens do not key or activate...
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SY09-03 Phytoestrogens are naturally occurring diphenolic compounds from plants that are structurally similar to the hormone 17β-estradiol. Possible anticarcinogenic effects of these compounds in breast cancer are attributed to their capability to bind to estrogen receptors. However, large-scale epidemiological studies have been limited by difficulties in quantifying phytoestrogen intake. Previous assays for phytoestrogens in biological fluids have analysed for a limited number of analytes and have often suffered from complex, multi-stage sample preparation techniques. Using newly developed GC/MS and LC/MS methods incorporating triply 13C-labelled standards, seven phytoestrogens (daidzein, genistein, glycitein, O-desmethylangolensin (O-DMA), equol, enterodiol, and enterolactone), it is now possible to measure urinary and circulating levels of phytoestrogens more accurately 1,2. In the absence of food composition data (although analyses are ongoing for UK foods) either phytoestrogens in either ...
http://ThomasDeLauer.com. 4 Foods That Mess Up Your Hormones. 1. Soy - Specifically Non-Organic. According to the National Institutes of Health, we do not fully understand the health effects of pesticides, but their use is associated with cancer, neurological conditions, and diabetes. (9). Phytoestrogens are found in numerous plants, including legumes, broccoli, beans, and soy products. Studies have found both positive and negative effects of phytoestrogens, so these are not necessarily bad - have been found to inhibit certain types of cancer and improve metabolic parameters (1).. That said, phytoestrogens have a very similar structure to the estrogen found in our bodies. These phytoestrogens both connect to and stimulate estrogen receptors. Case Study. In the Avon Longitudinal Study of Pregnancy and Childhood, it was found that 51 of 7,928 boys born suffered from hypospadias, a conditions where the penis does not work and appears abnormal. It was found that boys born to vegetarian mothers who ...
Because the interaction of phytoestrogens with our hormones and receptors is so complex, the jury is still out on their benefits vs. potential harms. Here I present a detailed discussion of phytoestrogens and their impacts on breast cancer.
Estrogen is a hormone primarily involved in the development, maturation and function of the female reproductive system. Phytoestrogens are natural...
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This is a Phase 2b double-blind, randomized, placebo-controlled parallel-group study evaluating the safety and efficacy of arimoclomol (400 mg t.i.d.) compared to placebo. A safety lead-in phase will be employed to ensure the safety of all study volunteers.. Tier I (Safety Lead-in): During the enrollment period for the safety lead-in phase, 24 volunteers meeting inclusion/exclusion criteria will be randomized at 4 investigative sites. These volunteers will have weekly visits during the first 4 weeks after starting treatment. Pharmacokinetics (PK) will be performed at various timepoints throughout these 4 weeks. After the initial 4 weeks of treatment, visits will continue at 4-week intervals up to Week 36, subsequently visits will occur every 8 weeks up to Week 68. A final visit will occur at Week 72. There will be a 28-day post study medication Follow-Up Telephone Call to assess medical status and adverse events.. Tier II: After the Tier I volunteers finish 4 weeks of treatment, their data will ...
Doctors help you with trusted information about Fibroids in Fibroid: Dr. Derman on fibroids uterine growth pregnancy: Only in very rare instances can fibroids be cancerous. If there is very rapid growth in the size, these are the ones that could be a leiomyosarcomas but again very rare.
The effect of doses of estradiol ranging from 0-0125 to 1-6 mug on the uterine weight of the spayed rat was studied 24 h after a… Expand ...
Ever wanted to create your own custom ROM but dont have enough coding experience to actually get started? Luckily, XDA Senior Moderator Dsixda has created a wonderful kitchen for HTC Android devices that should fulfill all of your ROM baking needs. This kitchen is compatible with both Linux and Mac OS X, so if youre running Windows, you will need to use either Cygwin or a virtualization technology such as VirtualBox. Details for getting either of those solutions are available in the Kitchen FAQ.. According to the developer, this kitchen is targeted towards:. ...
Phytoestrogens are diphenolic, non steroidal compounds which are present in plants and are consumed by both humans and animals. Previous investigations have demonstrated that dietary phytoestrogens appear to have neurobehavioral effects on intermale aggression in male Syrian hamsters and the neural mechanisms require further exploration. In this study experiments involving a phytoestrogen (PE) and a phytoestrogen free (PE Free) diet were performed to determine whether or not diet had an effect on the expression of a and p estrogen receptors in the brain of male Syrian hamsters (Mesocricetus auratus). Twenty male hamsters were used for experimentation and animals were divided into a PE (n = 10) and a PE Free group (n = 10) for a period of 3 weeks. Animals were sacrificed, perfused and brains removed for subsequent protein extraction and immunohistochemisty. Estrogen receptors were quantified using western blot utilizing brains from both the PE and the PE Free group. Results revealed a wide spread
The strong male predominance in esophageal and gastroesophageal junctional adenocarcinoma remains unexplained. Sex hormonal influence has been suggested, but not proven. A protective role of dietary phytoestrogen lignans was hypothesized. A Swedish nationwide population-based case-control study was conducted in 1995-1997, including 181 cases of esophageal adenocarcinoma, 255 cases of gastroesophageal junctional adenocarcinoma, 158 cases of esophageal squamous cell carcinoma, and 806 control subjects. Data on various exposures, including dietary data, were collected through personal interviews and questionnaires. Dietary intake of lignans was assessed using a food frequency questionnaire and categorized into quartiles based on the consumption among the control participants. Unconditional logistic regression was used to calculate odds ratios (ORs) with 95 % confidence intervals (CIs), including adjustment for all established risk factors. Participants in the highest quartile of intake of lignans ...
There seems to be a common misconception in the natural health community that women with high estrogen levels should take phytoestrogen (plant estrogen) because phytoestrogens are "protective estrogens" which reduce estrogenic activity. It is believed that phytoestrogens compete with endogenous estrogens for estrogen receptors. When the estrogen receptors are occupied by phytoestrogens, cell divisions are reduced because phytoestrogens are "weak estrogens". This theory made sense to me until my own experience showed otherwise. With my endometriosis, my pain level was a measurement for my condition. When I tried Don Quei, Licorice, Ginseng and Royal Jelly, I did feel better initially for a few weeks because they helped my blood circulation. As I continued to take these remedies, my pain would come back with a vengeance, more severe than before. I realized that weak estrogens could add up significantly if given enough time. I believe that many women are misled by initial feelings of wellness ...
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The once thriving captive-born southern white rhinoceros population is being threatened by their diet, according to new research. San Diego Zoo Global researchers predict that phytoestrogens in the rhinoceros´ food may be causing reproductive failure in the females.. In a press release detailing the research, Christopher Tubbs, researcher with the San Diego Zoo Institute for Conservation Research explained the importance of the study.. "Understanding why the captive white rhinoceros population has been dwindling for decades is an important part of protecting the future of this species. Our work is the first step toward determining if phytoestrogens are involved in this phenomenon and whether we need to reevaluate captive white rhino diets.". Zoologists have only recently found the northern and southern white rhinoceros to be different species´ rather than a subspecies of the African white rhinoceros. As such, conservation efforts have been specifically designed to increase the population of ...
Learn more about Estrogen at Doctors Hospital of Augusta Folate -Supplementation Possibly Helpful Ipriflavone -...
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Buy ESTROLOGIC - 60 caps Online.ESTROLOGIC is a special formula that contains extracts of plants rich in phytoestrogens or isoflavones, with which the organism will maintain the balance of oestrogen levels and help with the prevention
Scandium triflate is an effective pre-catalyst for the deoxygenative allylation of benzylic alcohols with a narrow substrate window. The reaction is shown to proceed through a
TY - JOUR. T1 - The role of soy phytoestrogens in prostate cancer. AU - Castle, Erik P. AU - Thrasher, J. Brantley. PY - 2002. Y1 - 2002. N2 - Epidemiological data of phytoestrogens and prostate cancer strongly supports the cancer protective effects of isoflavones found in soy products. Inhibition of cell proliferation via hormone-dependent and hormone-independent mechanisms by soy phytochemicals has been studied extensively in cell culture and animal studies. Herein, we review the current literature on the epidemiology and effects of two soy phytoestrogens, genistein and daidzein, and would stress the need for controlled human trials to assess the true preventive and therapeutic effects of these compounds.. AB - Epidemiological data of phytoestrogens and prostate cancer strongly supports the cancer protective effects of isoflavones found in soy products. Inhibition of cell proliferation via hormone-dependent and hormone-independent mechanisms by soy phytochemicals has been studied extensively ...
Abstract: Increasing concerns that environmental contaminants may disrupt the endocrine system require development of mathematical tools to predict the potential for such compounds to significantly alter human endocrine function. The endocrine system is largely self-regulating, compensating for moderate changes in dietary phytoestrogens (e.g., in soy products) and normal variations in physiology. However, severe changes in dietary or oral exposures or in health status (e.g., anorexia) , can completely disrupt the menstrual cycle in women. Thus, risk assessment tools should account for normal regulation and its limits. We present a mathematical model for the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in women as a function of estrogen, progesterone, and inhibin blood levels. The model reproduces the time courses of LH and FSH during the menstrual cycle and correctly predicts observed effects of administered estrogen and progesterone on LH and FSH ...
PLEASE NOTE: The Physical Assessment Entry Exam will be February 22,2016, at 8:00AM (Indianapolis time). CPR/AED must be obtained by the officer on his own and then show proof of completion before he will receive certification. Breath Tests for Intoxication has been removed from our currirulum. Departments must contact the Department of Toxicology, after the officer graduates from the LETB basic course, to schedule a date and time for training. Some of the new subjects that have been added is N.I.M.S. (Incident Management System Number 100 and 700); Human Trafficking; and Physical Training. This is just a few of the changes we will make in our curriculum. The daily schedule of this course and the Reporting Instructions can be accessed through the Calendar on our website . If you have any questions and/or comments please contact Captain Mark Bridge at (317)837-3228 or by email [email protected] The Calendar of Events is only a brief course description. Reporting instructions contain the
TY - JOUR. T1 - Genistein alters methylation patterns in mice. AU - Kevin Day, J.. AU - Bauer, Andrew M.. AU - Desbordes, Charles. AU - Zhuang, Yi. AU - Kim, Byung Eun. AU - Newton, Leslie G.. AU - Nehra, Vedika. AU - Forsee, Kara M.. AU - MacDonald, Ruth S.. AU - Besch-Williford, Cynthia. AU - Huang, Hui-ming. AU - Lubahn, Dennis B.. PY - 2002. Y1 - 2002. N2 - In this study we examine the effect of the phytoestrogen genistein on DNA methylation. DNA methylation is thought to inhibit transcription of genes by regulating alterations in chromatin structure. Estrogenic compounds have been reported to regulate DNA methylation in a small number of studies. Additionally, phytoestrogens are believed to affect progression of some human diseases, such as estrogen-dependent cancers, osteoporosis and cardiovascular disease. Specifically, our working hypothesis is that certain soy phytoestrogens, such as genistein, may be involved in preventing the development of certain prostate and mammary cancers by ...
Soy allergies are rare. The American College of Allergy, Asthma and Immunology estimate less than 1% of all children have a soy allergy.
Balance Plus Therapy an all natural Bio-identical USP progesterone cream features estrogen-like herbs known as phytoestrogens. It is specially formulated for women in menopause and beyond, needing hot flash relief daily and/or other symptoms of estrogen deficiency. Ideal following a hysterectomy for hormone therapy.
To quantify the effects of decreased cellular Tmod3 levels on cell morphology, the mean cell height and cross-sectional area were measured randomly throughout the sample while viewing only the channel showing F-actin; each measurement was later assigned to `transfected or `untransfected while viewing all channels. Expression of the silencing vector resulted in a highly significant decrease in cell height of approximately 30%, and a highly significant increase in cross-sectional area of approximately 40% (Fig. 2J,K; P,0.005). There was also a significant decrease (∼20%) in total F-actin fluorescence intensity in cells expressing the silencing vector, as determined by comparing the average fluorescence intensity in XY projections of image stacks of GFP-expressing transfected cells with untransfected cells in the same fields of view (Fig. 2L; P,0.05). There was no difference in height, cross-sectional area or total F-actin intensity between the untransfected control cells and cells transfected ...
Originally Posted by Family_guy This made me think of something interesting. So to play the devils advocate here dont you think there are other
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Coumestrol has about the same binding affinity for the ER-β estrogen receptor as 17β-estradiol, but much less affinity than 17α ... Coumestans, including coumestrol, a phytoestrogen, are found in a variety of plants. Food sources high in coumestans include ... Coumestrol Wedelolactone Plicadin Singh, Rishi Pal; Singh, Daljeet (1985). "An elegant synthesis of 6H-benzofuro[3,2-c][1] ... although the estrogenic potency of coumestrol at both receptors is much less than that of 17β-estradiol. Because of the ...
Red clover contains coumestrol, a phytoestrogen. Due to its activity on oestrogen receptors, the red clover is contraindicated ...
In plants which produce the phytoestrogen coumestrol, such as alfalfa, damage by aphids is linked with higher concentrations of ... Studies on the Chemical and Biological Properties of Coumestrol and Related Compounds. US Government Printing Office. pp. 47-67 ... coumestrol. The coating of plants with honeydew can contribute to the spread of fungi which can damage plants. Honeydew ...
Structurally, psoralidin is a coumestan derivative; it has an isopentenyl group at the second carbon position of coumestrol. ...
... coumestrol, daidzein, genistein, miroestrol). Those produced by fungi are known as mycoestrogens (e.g., zearalenone). Estrogens ...
Isoflavanes Isoflavandiols Isoflavenes Pterocarpans or Coumestans (phytoestrogens) Coumestrol red clover, alfalfa sprouts, soy ...
Evidence does not support a benefit from phytoestrogens such as coumestrol, femarelle, or the non-phytoestrogen black cohosh. ...
... s have also been shown to contain the phytoestrogen coumestrol, which has a variety of possible health effects. " ...
... coumestrol, kwakhurin, and mirificine), β-sitosterol, stigmasterol, campesterol, and mirificoumestan.[citation needed] There is ...
... anhydrovariabilin and coumestrol dimethyl ether. "The Plant List". Hyde, M. A.; Wursten, B. T.; Ballings, P.; Coates Palgrave, ...
... coumestrol, daidzein, deoxymiroestrol, equol, genistein, miroestrol, many others Selective estrogen receptor modulator ...
... coumestrol MeSH D03.438.150.266.450.400.375 --- genistein MeSH D03.438.150.266.450.400.687 --- pterocarpans MeSH D03.438. ... coumestrol MeSH D03.438.150.446.400 --- esculin MeSH D03.438.150.446.520 --- 4-hydroxycoumarins MeSH D03.438.150.446.520.079 ... coumestrol MeSH D03.830.219.266.450.400.375 --- genistein MeSH D03.830.219.266.450.400.687 --- pterocarpans MeSH D03.830. ... coumestrol MeSH D03.830.219.446.400 --- esculin MeSH D03.830.219.446.520 --- 4-hydroxycoumarins MeSH D03.830.219.446.520.079 ...
... coumestrol, daidzein, genistein, miroestrol) Selective estrogen receptor modulators (e.g., tamoxifen, clomifene, raloxifene) ...
... coumestrol - CP-358,774 - CP-609,754 - CP-724,714 - CP4071 - CpG 7909 - CPT 11 - CQS - craniopharyngioma - craniotomy - ...
... that both deoxymiroestrol and miroestrol were comparable in activity in vitro to other known phytoestrogens such as coumestrol ...
... has been shown to accelerate the onset of puberty in mice. Exposure to coumestrol immediately after birth resulted ... Coumestrol is a natural organic compound in the class of phytochemicals known as coumestans. Coumestrol was first identified as ... Levels of coumestrol within individual plants of the same species are variable. Studies of coumestrol levels in alfalfa suggest ... The highest concentrations of coumestrol are found in clover, Kala Chana, a type of chick pea, and Alfalfa sprouts. Coumestrol ...
Coumestans (e.g., coumestrol, psoralidin). *Flavonoids (incl. 7,8-DHF, 8-prenylnaringenin, apigenin, baicalein, baicalin, ...
Coumestans (e.g., coumestrol, psoralidin). *Flavonoids (incl. 7,8-DHF, 8-prenylnaringenin, apigenin, baicalein, baicalin, ... Plant products with estrogenic activity are called phytoestrogens (e.g., coumestrol, daidzein, genistein, miroestrol). ...
Coumestans (e.g., coumestrol, psoralidin). *Flavonoids (incl. 7,8-DHF, 8-prenylnaringenin, apigenin, baicalein, baicalin, ...
Coumestans (e.g., coumestrol, psoralidin). *Flavonoids (incl. 7,8-DHF, 8-prenylnaringenin, apigenin, baicalein, baicalin, ...
Coumestrol, an isoflavone coumarin derivative, is the only coumestan in foods.[175][176] ...
Coumestrol has been shown to accelerate the onset of puberty in mice. Exposure to coumestrol immediately after birth resulted ... Coumestrol is a natural organic compound in the class of phytochemicals known as coumestans. Coumestrol was first identified as ... Levels of coumestrol within individual plants of the same species are variable. Studies of coumestrol levels in alfalfa suggest ... The highest concentrations of coumestrol are found in clover, Kala Chana, a type of chick pea, and Alfalfa sprouts. Coumestrol ...
This topic has 1 study abstract on Coumestrol indicating that it may have therapeutic value in the treatment of Lupus ... Coumestrol from the national cancer Institutes natural product library is a novel inhibitor of protein kinase CK2.Jul 10, 2013 ... Coumestrol suppresses the accumulation of HIF-1α via suppression of SPHK1 pathway in hypoxic prostate cancer cells.May 31, 2014 ... Coumestrol treatment has potential benefits for either prevention or therapeutics use against cerebral ischemia in males.Feb 28 ...
High-performance liquid chromatographic assay of isoflavonoids and coumestrol from human urine.. Franke AA1, Custer LJ. ... and of coumestrol, another commonly occurring phytoestrogen. Analytes were isolated and concentrated by solid-phase extraction ... and separated by HPLC followed by identification through retention times and UV scans, and in the case of coumestrol ...
Male rats were injected either with 100 μg coumestrol or DMSO... ... Coumestrol had no effect on weights of testes and sex accessory ... Whereas steady state levels of LHβ mRNA in coumestrol-treated rats did not differ from those of controls, steady state levels ... Male rats were injected either with 100 μg coumestrol or DMSO (controls) daily during their first 5 d of life. Pituitary gland ... Neonatal exposure to coumestrol, a phytoestrogen, does not alter spermatogenic potential in rats. ...
These findings suggest that the anti-cancer effect of coumestrol is due to the direct targeting of haspin kinase. Coumestrol ... Haspin kinase was identified as a direct target protein of coumestrol using kinase profiling analysis. Histone H3 is a direct ... In the present study, we investigated coumestrol, a natural compound exhibiting broad anti-cancer effects against skin melanoma ... Coumestrol Epigenetically Suppresses Cancer Cell Proliferation: Coumestrol Is a Natural Haspin Kinase Inhibitor. Jong-Eun Kim 1 ...
Clandestine Coumestrol. Clover and soybean sprouts are highest in a phytoestrogen called coumestrol, a coumestan that can bind ... Brussels sprouts and spinach also contain coumestrol at a lower level, according to researchers of the 2007 mini review ...
Table of Substrates, Inhibitors and Inducers (including: CYP Enzymes, Clinical index drugs, transporters, and examples of clinical substrates, inhibitors, and inducers).
... coumestrol, n = 3; prunetin, n = 1), and the androgen trans-androsterone (n = 1). The poisson GLM indicated that the number of ... coumestrol, daidzein, formononetin, prunetin, and glycitein), and sterols (cholesterol, dihydrocholesterol, and 3β-coprostanol ... coumestrol, daidzein, formononetin, and prunetin) detected, total organic EDCs detected (classical estrogens + phytoestrogens ...
... coumestrol, ICI-164384 , 17 beta-estradiol , estrone, genistein , estriol , nafoxidine, 5-androstene-3 beta, 17 beta-diol , 17 ... coumestrol, ICI-164384 , estrone, 17 alpha-estradiol , nafoxidine, moxestrol , clomifene , estriol, 4-OH-estradiol , tamoxifen ...
Tahara 1987 coumestrolIngham 1988 mirificin, and 2 steroids, estrone and estriol.Sookvanichsilp 2008, Ingham 1986, Ingham 1989 ... coumestrol; genistein/equol; daidzein; resveratrol.Matsumura 2005P. mirifica also had an estrogenic effect on a human HepG2 ...
Coumestrol (CM), a phytoestrogen, and tetrachlorodioxin (TCDD) in concentrations of 10-4 to 10-6M significantly inhibited ... coumestrol and BPA are shown in Figure 3. 17-β-estradiol and coumestrol had significant cell cycle phase effect on actively ... Lower concentrations of coumestrol in long term exposure show increased cell accumulation in S phase and G2M (G2M shift from 10 ... Figure 7. RT-PCR of bcl-2 and p53 total Jurkat T cell RNA after 24 hours exposure to 10-6M 17-β-estradiol (E), coumestrol (CM ...
The science is complex, but imagine tiny messengers landing on a plant leaf in a light mist of water to hold urgent face-to-face meetings with the receptionists waiting on the plants surface.. Their news is so important, the receptionists signal the plants cells, then the whole plant sits up and takes note. It rapidly produces the essential oils needed to gets all its parts moving and swings into action. It marshals its defence forces, repairs breaches in the walls, and builds strength and vigour. If theres an attack, the plant is ready to go to war. If theres not, it is strongly motivated to become the best it can be. ...
Coumestrol Explained. Leave a Comment WHAT IS COUMESTROL? Coumestrol is a coumestan which is part of the phytonutrient (a group ... In this article I will be providing a full breakdown of coumestrol. WHEN WAS COUMESTROL DISCOVERED? Coumestrol was first ... Filed Under: Coumestans Tagged With: antioxidants, coumestans, coumestrol, flavonoids, phenolic compounds, phytochemicals, ... Coumestans (which include coumestrol). - Flavanones (which include hesperidin, naringenin and silymarin). - … [Read more...] ...
Coumestrol Explained. Leave a Comment WHAT IS COUMESTROL? Coumestrol is a coumestan which is part of the phytonutrient (a group ... In this article I will be providing a full breakdown of coumestrol. WHEN WAS COUMESTROL DISCOVERED? Coumestrol was first ... Filed Under: Coumestans Tagged With: antioxidants, coumestans, coumestrol, flavonoids, phenolic compounds, phytochemicals, ... There is relatively little information available on coumestrol although it is believed to have strong cancer fighting ...
In the investigation in vitro of protective effects of coumestrol on mice astrocytes, found that coumestrol induced a modest ... In the investigation of the effects of coumestrol on osteoblasts and osteoclasts, inducated that phytoestrogen coumestrol has a ... but coumestrol had little effect on intestinal ALP activity at 10 days after parturition, according to "Effects of coumestrol ... M coumestrol, according to "The protective effects of coumestrol against amyloid-beta peptide- and lipopolysaccharide-induced ...
Coumestrol inhibits autoantibody production through modulating Th1 response in experimental autoimmune thyroiditis.Aug 15, 2016 ...
The phytoestrogen coumestrol decreases autoantibodies in a mouse model of systemic lupus. Dec 01, 2004. ...
... coumestrol for 4%, and the lignans for 2%. The primary sources of phytoestrogens were tofu (47%), soy milk (22%), and foods ... coumestrol; and the lignans: matairesinol and secoisolariciresinol. ...
... coumestrol for 4%, and the lignans for 2%. The primary sources of phytoestrogens were tofu (47%), soy milk (22%), and foods ... coumestrol; and the lignans: matairesinol and secoisolariciresinol. ...
Coumestrol modulates Akt and Wnt/β-catenin signaling during the attenuation of adipogenesis.Dec 06, 2016. ...
Coumestrol modulates Akt and Wnt/β-catenin signaling during the attenuation of adipogenesis.Dec 06, 2016. ...
Additionally, coumestrol and genistein induced apoptosis in MECs. These results indicate that phytoestrogens weaken the blood- ... The results showed that coumestrol and genistein changed the expression of TJ proteins (claudins-3 and -4 and occludin), ... Therefore, we investigated the influence of phytoestrogens (coumestrol, genistein, and daidzein) by using an in vitro mouse-MEC ... 0 (Caseins); 0 (Isoflavones); 0 (Phytoestrogens); 6287WC5J2L (daidzein); DH2M523P0H (Genistein); V7NW98OB34 (Coumestrol). ...
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model. ...
Medicago 10% Coumestrol Milk Thistle 80% Silymarin (HPLC) Morinda Root 4:1 Extract Motherwort 5:1 Extract Moutan Bark 20:1 ...
Zimmermann, M., Tilghman, S.L., Boue, S.M., Salvo, V.A., Elliott, S., Williams, K., Skripnikova, E.V., Ashe, H., Payton-Stewart, F., Vanhoy-Rhodes, L., Fonseca, J., Corbitt, C., Collins-Burow, B.M., Howell, M.H., Lacey, M., Shih, B.Y., Carter Wientjes, C.H., Cleveland, T.E., Mclachlan, J.A., Wiese, T.E., Beckman, B.S., Burow, M.E. 2010. Glyceollin I, A Novel Antiestrogenic Phytoalexin Isolated from Activated Soy. Journal of Pharmacology and Experimental Therapeutics. 332(1):35-45 ...
  • Coumestrol is the most potent of the phytoestrogens, but is some 100-200 times less potent than 17β-oestradiol, and almost 3000 times less potent than diethylstilboestrol. (bmj.com)
  • A comparative study of the estrogenic properties of phytoestrogens found that both deoxymiroestrol and miroestrol were comparable in activity in vitro to other known phytoestrogens such as coumestrol as 17β-oestradiol agonists. (wikipedia.org)
  • Coumestrol is a coumestan which is part of the phytonutrient (a group of chemical compounds that occur naturally in plants and have multiple health benefits but are not considered essential to human health) family. (freefitnesstips.co.uk)
  • The Coumestan group includes coumestrol. (smart-publications.com)
  • Boué and colleagues 4 -for example, have shown that Aspergillus sojae induces the production of the coumestan phytoalexin, coumestrol, and the pterocarpan phytoalexins, glyceollins I-III in soybean cotyledons. (bmj.com)
  • Brussels sprouts and spinach also contain coumestrol at a lower level, according to researchers of the 2007 mini review published in 'Clinical Cases in Mineral and Bone Metabolism. (livestrong.com)
  • When neonatal female mice were treated with coumestrol, similar reproductive irregularities occurred, and at 22 months old, atypical collagen distribution was observed in the uterine wall. (wikipedia.org)
  • We conclude that neonatal exposure to coumestrol does not alter reproductive organ structure or spermatogenic potential in male rats. (springer.com)
  • Neonatal rats and immature rats exposed to coumestrol had premature estrous cycles. (anabolicminds.com)
  • Coumestrol Confers Partial Resistance in Soybean Plants Against Cercospora Leaf Blight. (pubfacts.com)
  • male rats that nursed from females with coumestrol in their diets were both less likely to mount a female rat and less likely to ejaculate, despite producing normal levels of testosterone. (wikipedia.org)
  • Female rats that were exposed to coumestrol neonatally did not adopt the lordosis position as much as those that were not exposed to coumestrol. (wikipedia.org)
  • Coumestrol has neuroprotective effects before and after global cerebral ischemia in female rats. (greenmedinfo.com)
  • Male rats were injected either with 100 μg coumestrol or DMSO (controls) daily during their first 5 d of life. (springer.com)
  • Similarly, there were no significant differences among serum concentrations of testosterone, LHβ and FSH of coumestrol-treated rats and those of controls. (springer.com)
  • Whereas steady state levels of LHβ mRNA in coumestrol-treated rats did not differ from those of controls, steady state levels of FSHβ mRNA increased (37%) in treated animals. (springer.com)
  • In the estimation the risk of gastric cancer (GC) in relation to the individual and combined consumption of polyphenols and NOC precursors (nitrate and nitrite), found that for the first time, a protective effect for GC because of higher intake of cinnamic acids, secoisolariciresinol and coumestrol , and suggest that these polyphenols reduce GC risk through inhibition of endogenous nitrosation. (blogspot.com)
  • High-performance liquid chromatographic assay of isoflavonoids and coumestrol from human urine. (nih.gov)
  • The chemical shape of coumestrol orients its two hydroxy groups in the same position as the two hydroxy groups in estradiol, allowing it to inhibit the activity of aromatase and 3α-hydroxysteroid dehydrogenase. (wikipedia.org)
  • Coumestrol (CM), a phytoestrogen, and tetrachlorodioxin (TCDD) in concentrations of 10 -4 to 10 -6 M significantly inhibited Jurkat T cell lymphoproliferation, whereas bisphenol A (BPA) and DDT had minimal effect, but did antagonize 17-β-estrtadiol induced effects. (mdpi.com)
  • In the evaluation of Endogenous estrogens dramatic and differential effects on classical endocrine organ and proliferation, found that Using human cervical cancer cells (HeLa cells) as a model, the effects of representative xenoestrogens ( Coumestrol -a phytoestrogen, tetrachlorodioxin (TCDD)-a herbicide and DDT-a pesticide) on proliferation, cell cycle, and apoptosis were examined. (blogspot.com)
  • In the clarification of the effects of coumestrol administration on Ca metabolism during pregnancy and in lactating mice, found that coumestrol did not affect serum Ca and the expression of vitamin D receptor protein in the duodenum and jejunum. (blogspot.com)
  • Antiobesity effects of coumestrol through expansion and activation of brown adipose tissue metabolism. (nih.gov)
  • In the investigation in vitro of protective effects of coumestrol on mice astrocytes, found that coumestrol induced a modest but significant increase in viability of astrocytes, while the viability of astrocytes was reduced following exposure to LPS and amyloid-beta peptide. (blogspot.com)
  • Analytes were isolated and concentrated by solid-phase extraction and separated by HPLC followed by identification through retention times and UV scans, and in the case of coumestrol additionally by fluorometric response. (nih.gov)
  • Coumestrol found in sunflower seeds when fed to the mother rat caused permanent reproductive problems in the rat pups: female pups when grown did not ovulate, and the male pups had altered mounting behavior and fewer ejaculations. (anabolicminds.com)
  • Exposure to coumestrol immediately after birth resulted in an initial increase in uterine weight. (wikipedia.org)
  • Coumestrol has been shown to accelerate the onset of puberty in mice. (wikipedia.org)
  • In the present study, we investigated coumestrol, a natural compound exhibiting broad anti-cancer effects against skin melanoma, lung cancer and colon cancer cell growth. (mdpi.com)
  • Coumestrol inhibits autoantibody production through modulating Th1 response in experimental autoimmune thyroiditis. (greenmedinfo.com)
  • Coumestrol had no effect on weights of testes and sex accessory organs, or sperm count. (springer.com)
  • These findings suggest that the anti-cancer effect of coumestrol is due to the direct targeting of haspin kinase. (mdpi.com)
  • The addition of coumestrol could reverse the toxic effect induced by LPS and amyloid-beta peptide. (blogspot.com)