Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Any method used for determining the location of and relative distances between genes on a chromosome.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The functional hereditary units of BACTERIA.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A form of GENE LIBRARY containing the complete DNA sequences present in the genome of a given organism. It contrasts with a cDNA library which contains only sequences utilized in protein coding (lacking introns).
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.
A contiguous gene syndrome associated with hemizygous deletions of chromosome region 11p13. The condition is marked by the combination of WILMS TUMOR; ANIRIDIA; GENITOURINARY ABNORMALITIES; and INTELLECTUAL DISABILITY.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.
A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The type species of VARICELLOVIRUS causing CHICKENPOX (varicella) and HERPES ZOSTER (shingles) in humans.
A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.
One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence G/AATTC at the slash. EcoRI is from E coliRY13. Several isoschizomers have been identified. EC 3.1.21.-.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
One of the Type II site-specific deoxyribonucleases (EC 3.1.21.4). It recognizes and cleaves the sequence A/AGCTT at the slash. HindIII is from Haemophilus influenzae R(d). Numerous isoschizomers have been identified. EC 3.1.21.-.
The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.
The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.

Analysis of two cosmid clones from chromosome 4 of Drosophila melanogaster reveals two new genes amid an unusual arrangement of repeated sequences. (1/1067)

Chromosome 4 from Drosophila melanogaster has several unusual features that distinguish it from the other chromosomes. These include a diffuse appearance in salivary gland polytene chromosomes, an absence of recombination, and the variegated expression of P-element transgenes. As part of a larger project to understand these properties, we are assembling a physical map of this chromosome. Here we report the sequence of two cosmids representing approximately 5% of the polytenized region. Both cosmid clones contain numerous repeated DNA sequences, as identified by cross hybridization with labeled genomic DNA, BLAST searches, and dot matrix analysis, which are positioned between and within the transcribed sequences. The repetitive sequences include three copies of the mobile element Hoppel, one copy of the mobile element HB, and 18 DINE repeats. DINE is a novel, short repeated sequence dispersed throughout both cosmid sequences. One cosmid includes the previously described cubitus interruptus (ci) gene and two new genes: that a gene with a predicted amino acid sequence similar to ribosomal protein S3a which is consistent with the Minute(4)101 locus thought to be in the region, and a novel member of the protein family that includes plexin and met-hepatocyte growth factor receptor. The other cosmid contains only the two short 5'-most exons from the zinc-finger-homolog-2 (zfh-2) gene. This is the first extensive sequence analysis of noncoding DNA from chromosome 4. The distribution of the various repeats suggests its organization is similar to the beta-heterochromatic regions near the base of the major chromosome arms. Such a pattern may account for the diffuse banding of the polytene chromosome 4 and the variegation of many P-element transgenes on the chromosome.  (+info)

Nonmethylated transposable elements and methylated genes in a chordate genome. (2/1067)

The genome of the invertebrate chordate Ciona intestinalis was found to be a stable mosaic of methylated and nonmethylated domains. Multiple copies of an apparently active long terminal repeat retrotransposon and a long interspersed element are nonmethylated and a large fraction of abundant short interspersed elements are also methylation free. Genes, by contrast, are predominantly methylated. These data are incompatible with the genome defense model, which proposes that DNA methylation in animals is primarily targeted to endogenous transposable elements. Cytosine methylation in this urochordate may be preferentially directed to genes.  (+info)

Three receptor genes for plasminogen related growth factors in the genome of the puffer fish Fugu rubripes. (3/1067)

Plasminogen related growth factors (PRGFs) and their receptors play major roles in embryogenesis, tissue regeneration and neoplasia. In order to investigate the complexity and evolution of the PRGF receptor family we have cloned and sequenced three receptors for PRGFs in the teleost fish Fugu rubripes, a model vertebrate with a compact genome. One of the receptor genes isolated encodes the orthologue of mammalian MET, whilst the other two may represent Fugu rubripes orthologues of RON and SEA. This is the first time three PRGF receptors have been identified in a single species.  (+info)

Complete exon-intron organization of the mouse fibulin-1 gene and its comparison with the human fibulin-1 gene. (4/1067)

Fibulin-1 is a 90 kDa calcium-binding protein present in the extracellular matrix and in the blood. Two major variants, C and D, differ in their C-termini as well as the ability to bind the basement membrane protein nidogen. Here we characterized genomic clones encoding the mouse fibulin-1 gene, which contains 18 exons spanning at least 75 kb of DNA. The two variants are generated by alternative splicing of exons in the 3' end. By searching the database we identified most of the exons encoding the human fibulin-1 gene and showed that its exon-intron organization is similar to that of the mouse gene.  (+info)

Genetic variation of chlorella viruses: variable regions localized on the CVK2 genomic DNA. (5/1067)

A physical map of the Chlorella virus CVK2 genomic DNA has been constructed based on a cosmid contig covering the entire genomic region. By using Southern blot analysis with 22 gene probes, the gene arrangement along the genome was compared between CVK2 and PBCV-1, the prototypic member of Phycodnaviridae, whose genomic sequence is now available. The major rearrangements were (1) an insertion of a 20-kbp region around the left end of CVK2 DNA, (2) a duplication of the gene for major capsid protein in CVK2 DNA, (3) deletions/insertions of some open reading frames, and (4) divergence in the terminal inverted repeat sequences. Despite these changes, extensive colinearity was revealed between most of the genes along the CVK2 and PBCV-1 genomes. These data imply that the Chlorella virus genome has an overall high degree of genomic stability, encompassing specific islands of rearrangements.  (+info)

Comparative genomic analysis of the interferon/interleukin-10 receptor gene cluster. (6/1067)

Interferons and interleukin-10 are involved in key aspects of the host defence mechanisms. Human chromosome 21 harbors the interferon/interleukin-10 receptor gene cluster linked to the GART gene. This cluster includes both components of the interferon alpha/beta-receptor (IFNAR1 and IFNAR2) and the second components of the interferon gamma-receptor (IFNGR2) and of the IL-10 receptor (IL10R2). We report here the complete gene content of this GART-cytokine receptor gene cluster and the use of comparative genomic analysis to identify chicken IFNAR1, IFNAR2, and IL10R2. We show that the large-scale structure of this locus is conserved in human and chicken but not in the pufferfish Fugu rubripes. This establishes that the receptor components of these host defense mechanisms were fixed in an ancestor of the amniotes. The extraordinary diversification of the interferon ligand family during the evolution of birds and mammals has therefore occurred in the context of a fixed receptor structure.  (+info)

Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication. (7/1067)

Human DNA topoisomerase II is essential for chromosome segregation and is the target for several clinically important anticancer agents. It is expressed as genetically distinct alpha and beta isoforms encoded by the TOP2alpha and TOP2beta genes that map to chromosomes 17q21-22 and 3p24, respectively. The genes display different patterns of cell cycle- and tissue-specific expression, with the alpha isoform markedly upregulated in proliferating cells. In addition to the fundamental role of TOP2alpha and TOP2beta genes in cell growth and development, altered expression and rearrangement of both genes are implicated in anticancer drug resistance. Here, we report the complete structure of the human topoisomerase IIalpha gene, which consists of 35 exons spanning 27.5 kb. Sequence data for the exon-intron boundaries were determined and examined in the context of topoisomerase IIalpha protein structure comprising three functional domains associated with energy transduction, DNA breakage-reunion activity and nuclear localization. The organization of the 3' half of human TOP2beta, including sequence specifying the C-terminal nuclear localization domain, was also elucidated. Of the 15 introns identified in this 20 kb region of TOP2beta, the first nine and the last intron align in identical positions and display the same phases as introns in TOP2alpha. Though their extreme 3' ends differ, the striking conservation suggests the two genes diverged recently in evolutionary terms consistent with a gene duplication event. Access to TOP2alpha and TOP2beta gene structures should aid studies of mutations and gene rearrangements associated with anticancer drug resistance.  (+info)

Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis. (8/1067)

Higher plant reproduction is unique because two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that replicates to generate the endosperm, a tissue that supports embryo development. To understand mechanisms that initiate reproduction, we isolated a mutation in Arabidopsis, f644, that allows for replication of the central cell and subsequent endosperm development without fertilization. When mutant f644 egg and central cells are fertilized by wild-type sperm, embryo development is inhibited, and endosperm is overproduced. By using a map-based strategy, we cloned and sequenced the F644 gene and showed that it encodes a SET-domain polycomb protein. Subsequently, we found that F644 is identical to MEDEA (MEA), a gene whose maternal-derived allele is required for embryogenesis [Grossniklaus, U., Vielle-Calzada, J.-P., Hoeppner, M. A. & Gagliano, W. B. (1998) Science 280, 446-450]. Together, these results reveal functions for plant polycomb proteins in the suppression of central cell proliferation and endosperm development. We discuss models to explain how polycomb proteins function to suppress endosperm and promote embryo development.  (+info)

Yeah you could use the simple protocl for transformation like one given in manniatis involving cacl2 treated competent cells. I have used this method for transforming 21 kb cosmid. I hope this helps ...
TY - JOUR. T1 - A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21. AU - Couch, Fergus J.. AU - Castilla, Lucio H.. AU - Xu, Junzhe. AU - Abel, Kenneth J.. AU - Welcsh, Piri. AU - King, Stephanie E.. AU - Wong, Linghua. AU - Ho, Peggy P.. AU - Merajver, Sofia. AU - Brody, Lawrence C.. AU - Yin, Guiying. AU - Hayes, Steve T.. AU - Gieser, Linn M.. AU - Flejter, Wendy L.. AU - Glover, Thomas W.. AU - Friedman, Lori S.. AU - Lynch, Eric D.. AU - Meza, Jose E.. AU - King, Mary Claire. AU - Law, David J.. AU - Deaven, Larry. AU - Bowcock, Anne M.. AU - Collins, Francis S.. AU - Weber, Barbara L.. AU - Chandrasekharappa, Settara C.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - A familial early-onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. To characterize this region and to aid in the identification of the BRCA1 gene, a physical map of a region of 1.0-1.5 Mb between the EDH17B1 and the PPY loci on chromosome 17q21 was generated. The physical map is ...
The set of clone ends is dumped as part of the gff files: http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE/GFF_files.shtml This is the source for the extents displayed in WormBase. The caveat with this is that the true end is not marked up for all clones. The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left and right ends as this became part of the standard procedure. Finally, many of the YACs do not have clone ends because the segment submitted to GenBank/EMBL is much smaller than the full clone, and hence the true ends lie within sequences already finished at that stage of the sequencing (i.e. we never went back to update clone ends in sequence already finished ...
The set of clone ends is dumped as part of the gff files: http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE/GFF_files.shtml This is the source for the extents displayed in WormBase. The caveat with this is that the true end is not marked up for all clones. The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left and right ends as this became part of the standard procedure. Finally, many of the YACs do not have clone ends because the segment submitted to GenBank/EMBL is much smaller than the full clone, and hence the true ends lie within sequences already finished at that stage of the sequencing (i.e. we never went back to update clone ends in sequence already finished ...
A few weeks ago I described some problems we were having with cosmid libraries. I got several good responses. The one below was the best and might be useful to others. What you have been seeing is actually the gradual loss of the selection pressure, which results from the degradation of the ampicillin in the medium by the beta-lactamase produced by the amp- containing clone. This eventually leads to the loss of the amp- containing cosmid. You might recall the growth of satellite colonies around the genuine transformants when you let the incubation of transformation plates go longer, say two days instead of overnight. After 4-6 weeks the clone has lost the amp-containing cosmid and becomes ampicillin sensitive. That is why you transferred the cells to ampicillin-containing liquid medium and you did not see any growth the next day. The same goes for the lower yield of cosmid DNA after 3-4 weeks, since the colony contains a population of resistant and sensitive cells. My suggestion to you is to ...
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pWEB::TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies,The pWEB::TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of genomic DNA for subsequent use in making nested deletion sublibraries. High efficiency cosmid library construction is accomplished using easy and highly repr,biological,biology supply,biology supplies,biology product
10 mg transfection-grade endofree plasmid or cosmid DNA, HiSpeed Mega, HiSpeed Giga, EF plasmid, HiSpeed Mega and Giga EF Plasmid Kits
The DHFR-G8 cell line was produced by M.C. Hung, et al., in 1986 from the NIH/3T3 mouse fibroblast cell line which was cotransfected with the cNEU-p clone and the pSV2-DHFR plasmid. This cell line was developed from one methotrexate resistant colony by selection in 0.6 uM methotrexate and 10% dialyzed calf serum. These cells contain 50-100 copies of the cNeu-P (normal rat cosmid DNA) and produce approximately 4 x 10(5) molecules of encoded p185 protein per cell.
SpinSmart Plasmid Miniprep kits are designed to rapidly purify plasmid DNA from bacterial cultures.The protocol below is appropriate for both CM-410-50 and CM-410-250.
Ji H, Smith L.M, Guilfoyle RA. 1994. Rapid isolation of cosmid insert DNA by triple-helix-mediated affinity capture. Genetic Analysis, Techniques and Applications. 11:43-7. ...
Ji H, Smith L.M, Guilfoyle RA. 1994. Rapid isolation of cosmid insert DNA by triple-helix-mediated affinity capture. Genetic Analysis, Techniques and Applications. 11:43-7. ...
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cosmid definition: Genetics A hybrid vector that has been spliced with plasmid DNA for cloning huge genes or gene fragments.; a kind of plasmid (often utilized as a cloning vector) constructed because…
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The method used for the isolation of large scale cosmid and plasmid DNA is an unpublished modification (16) of an alkaline lysis procedure (17,18) followed by equilibrium ultracentrifugation in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes are precipitated with sodium acetate, and the lysate is cleared first by filtration of precipitate through cheesecloth and then by centrifugation. The DNA-containing supernatant is transferred to a new tube, and the plasmid or cosmid DNA is precipitated by the addition of polyethylene glycol and collected by centrifugation. The DNA pellet is resuspended in a buffer containing cesium chloride and ethidium bromide, which is loaded into polyallomer tubes and subjected to ultracentrifugation overnight. The ethidium bromide stained plasmid or cosmid DNA bands, ...
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The NucleoBond BAC 100 Kit is designed to purify large DNA fragments such as cosmids, bacteriophage P1 clones, PACs, and BACs, without phenol/chloroform extraction. 1 hour protocol accomodates vectors up to to 300 kb.
Maxiprep NucleoBond Plasmid Kits: Purify plasmid DNA, cosmids, BACs, PACs, and YACs in just a few hours-no phenol/chloroform extraction. Use for transfection, in vitro transcription, and automated sequencing.
Kanamycin - The frozen stock solutions of kanamycin are at 50mg/ml in H2O, and are marked with green. The final concentration for LB liquid culture for growing plasmids is 50ug/ml, and for cosmids is 20ug/ml. To obtain 50ng/ml in 100ml of LB, add 100ul stock solution, and to obtain 20ug/ml, add 40ul stock solution ...
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Despite screening various growth media, we failed to detect the production of any 2-hydroxyphenylthiazoline-containing metabolites by S. venezuelae. This is potentially explained by the low levels of sven0516 expression in the bldM mutant (Fig. 3), which is surprising given that sven0517 is likely to be in the same operon, and possibly reflects differential mRNA stability for the two genes. We therefore elected to express the sven0503-sven0517 gene cluster in the engineered host S. coelicolor M1152.23 A clone (SV-2_E03) from an ordered genomic cosmid library of the S. venezuelae chromosome containing a segment extending from sven0496 to sven0518 was PCR-targeted in Escherichia coli with a 5.2 kb SspI fragment from pIJ10702 that contains oriT, and the øC31 integrase gene and phage attachment site (attP). The resulting cosmid, SV-2_E03::SspI, was introduced into S. coelicolor M1152 by conjugation, whereupon it integrated into the chromosomal øC31 attB site. Wild type S. coelicolor M1152 and the ...
TY - JOUR. T1 - Isolation and characterization of a novel gene deleted in digeorge syndrome. AU - Kurahashi, Hiroki. AU - Akagi, Kenzo. AU - Inazawa, Johji. AU - Ohta, Tohru. AU - Niikawa, Norio. AU - Kayatani, Futoshi. AU - Sano, Tetsuya. AU - Okada, Shintaro. AU - Nishisho, Isamu. PY - 1995/4/1. Y1 - 1995/4/1. N2 - The region commonly deleted in DiGeorge syndrome (DGS) has been localized at 22q11.1-q11.2 with the aid of a high resolution banding technique. A 22q11 specific plasmid library was constructed with a microdissection and microcloning method. Dosage analysis proved three of 144 randomly selected microclones to detect hemizygosity in two patients with DGS. Two of the clones were found to contain independent low-copy-number repetitive sequences, all of which were included in the region deleted in the DGS patients. Screening of the cosmid library and subsequent cosmid walking allowed us to obtain two cosmid contigs corresponding to the microclones within the deletion (contig 1 and contig ...
8-base recognition sites will yield pieces 64,000 bases long. Since hundreds of different restriction enzymes have been characterized, DNA can be cut into many different small fragments. Physical Maps Different types of physical maps vary in their degree of resolution. The lowest- resolution physical map is the chromosomal (sometimes called cytogenetic) map, which is based on the distinctive banding patterns observed by light microscopy of stained chromosomes. A cDNA map shows the locations of expressed DNA regions (exons) on the chromosomal map. The more detailed cosmid contig map depicts the order of overlapping DNA fragments spanning the genome. A macrorestriction map describes the order and distance between enzyme cutting (cleavage) sites. The highest- resolution physical map is the complete elucidation of the DNA base- pair sequence of each chromosome in the human genome. Physical maps are described in greater detail below. Low-Resolution Physical Mapping Chromosomal map. In a chromosomal ...
Empirical Bioscience announced the introduction of a new Plasmid Mini-Prep Kit today. Requiring only 1-3mL of bacterial culture, the kit isolates high-quality plasma or cosmid DNA for extraction up to 10 kb in length and yields up to 20 µg per preparation.. In addition to using smaller volumes of cultures, the kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches the optimal pH level for DNA binding, 7.5. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation.. Besides the color changing lysis buffer, the kit includes, neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns and collection tubes. These components create a versatile kit that lends itself well for use in ...
The data section can follow or proceed the configuration section. The two sections can also be intermixed. The data section is a tab or whitespace-delimited file which you can export from a spreadsheet application or word processor file (be sure to save as text only!). Here is an example data section:. Cosmid B0511 . 516-619 Cosmid B0511 . 3185-3294 Cosmid B0511 . 10946-11208 Cosmid B0511 . 13126-13511 Cosmid B0511 . 66-208 Cosmid B0511 . 6354-6499 Cosmid B0511 . 13955-14115 EST yk595e6.5 + 3187-3294 EST yk846e07.3 - 11015-11208 EST yk53c10 yk53c10.5 + 18892-19154 yk53c10.3 - 15000-15500,15700-15800 EST yk53c10.5 + 16032-16105 SwissProt PECANEX + 13153-13656 Swedish fish FGENESH Gene 1 - 1-205,518-616,661-735,3187-3365,3436-3846 Transmembrane domain FGENESH Gene 2 - 16626-17396,17451-17597 Kinase and sushi domains. Each line of the file contains five columns. The columns are:. ...
Cosmid clones containing alpha 1-antitrypsin (alpha 1AT) gene sequences were observed to contain alpha 1AT-like sequences approximately 12 kb downstream of the authentic alpha 1AT gene. Restriction mapping suggested the alpha 1AT-like gene lacks promoter sequences. Cosmid clones from one library con …
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Its another Monday and time to introduce another brand new Cosmid girl to you. Her name is Britta and she is from California. She is a 20 year old student that has a serious artsy vibe to her, front what I can tell though, she seems to be a little earthy, if you know what I mean. She is super nice and has such a great smile. She is 57 as well, so a pretty tall drink of water and have a very well proportioned body. I hope that you enjoy out new cutie.. ...
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TY - JOUR. T1 - Detection of submicroscopic chromosomal deletions in aniridia patients using fluorescence in situ hybridization and a panel of cosmids covering the WT1 gene. AU - Kempski, H.. AU - Cowell, J. K.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - A series of cosmids have been isolated from a human chromosome 11-specific cosmid library using the human Wilms tumour predisposition gene cDNA, WT33. Seven overlapping cosmids were isolated which cover the genomic sequence of WT1 and in situ hybridisation shows that they all localise to the p13 region of chromosome 11. Chromosomes from patients with aniridia and Wilms tumour, and a small subband deletion in 11p13, were analysed and no hybridisation signal was seen on the deletion chromosomes. These cosmids, therefore, can be used to analyse chromosomes from patients with sporadic aniridia for submicroscopic deletions. Aniridia patients who show normal hybridisation patterns on both chromosomes need no longer be screened for Wilms tumours.. AB - A ...
The I-2 locus in tomato confers resistance to race 2 of the soil-borne fungus Fusarium oxysporum f sp lycopersici. The selective restriction fragment amplification (AFLP) positional cloning strategy was used to identify I-2 in the tomato genome. A yeast artificial chromosome (YAC) clone covering ~750 kb encompassing the I-2 locus was isolated, and the AFLP technique was used to derive tightly linked AFLP markers from this YAC clone. Genetic complementation analysis in transgenic R1 plants using a set of overlapping cosmids covering the I-2 locus revealed three cosmids giving full resistance to F. o. lycopersici race 2. These cosmids shared a 7-kb DNA fragment containing an open reading frame encoding a protein with similarity to the nucleotide binding site leucine-rich repeat family of resistance genes. At the I-2 locus, we identified six additional homologs that included the recently identified I-2C-1 and I-2C-2 genes. However, cosmids containing the I-2C-1 or I-2C-2 gene could not confer ...
Empirical Bioscience announced the introduction of a new Plasmid Mini-Prep Kit today. Requiring only 1-3mL of bacterial culture, the kit isolates high-quality plasma or cosmid DNA for extraction up to 10 kb in length and yields up to 20 µg per preparation. In addition to using smaller volumes of cultures, the kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches the optimal pH level for DNA binding, 7.5. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation. Besides the color changing lysis buffer, the kit includes, neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns and collection tubes. These components create a versatile kit that lends itself well for use in ...
A series of 130 DNA probes, hybridized to the CIC YAC library, are ordered along the abscissa. A subset of 58 of the probes, mapped to chromosome 2 by RFLP analysis, serve as contact points between the genetic and physical maps. The positions of markers mapped to the recombinant inbred lines (7) are indicated in cM above the marker names. When a set of probes hybridized to the same YACs, their order is listed by their position in the genetic map (7). If no genetic or auxilliary data (e.g. cosmid contig (4)) was available, the exact probe order is indeterminate and is arbitrarily assigned.. A line drawn above a marker indicates a difference in marker order between the physical and genetic maps. Markers designated RI have been mapped by us to the recombinant inbred populations and are in correct relative position (with one exception, 15414, marked RI-?) to the markers on either side (no number is indicated as adding markers and using a different mapping program generates different absolute ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Brian Robertson wrote: , , The amount of bacterial genome data available as sequenced cosmids of , 30-40 kb is increasing rapidly. Our problem is that we need to keep track , of newly discovered genes as they appear, so they can be incorporated into , our research program as appropriate. For this we need to create lists of , probable genes identified in the annotations for each cosmid. This can , then be circulated to laboratory workers. , , An example of this kind of annotation is shown below. We would like to , extract the /note field, which contains the probable function of the , gene, and create a list of these for each cosmid. , , FT CDS_pept complement(3043..4155) , FT /note=MTCY190.03c, probable anthranilate , FT phosphoribosyltransferase, trpD, len: 370, similar to eg , FT SW:TRPD_LACCA P17170, (43.2% identity in 308 aa overlap), , FT initiation codon uncertain, gtg at 4086 favoured by , FT homology but this has no clear ribosome binding site , , Does anyone know of a way of ...
The kits utilize a novel strategy of cloning end-repaired, randomly sheared DNA instead of the conventional approach of cloning fragments generated by partial restriction endonuclease digestion. First, genomic DNA is sheared by passing it through a syringe needle. The sheared DNA is end-repaired to generate 5-phosphorylated blunt ends and size-selected using a low melting point agarose gel. The size-selected DNA is then ligated into the supplied linearized and dephosphorylated pWEB-TNCTM or pWEBTM Cosmid Vector, packaged using ultra-high efficiency MaxPlaxTM Lambda Packaging Extracts (,109 pfu/μg for phage lambda) and plated on phage T1-resistant EPI100TM-T1R E. coli plating cells, all included in the kit. The result is a complete and unbiased primary cosmid library ...
The kits utilize a novel strategy of cloning end-repaired, randomly sheared DNA instead of the conventional approach of cloning fragments generated by partial restriction endonuclease digestion. First, genomic DNA is sheared by passing it through a syringe needle. The sheared DNA is end-repaired to generate 5-phosphorylated blunt ends and size-selected using a low melting point agarose gel. The size-selected DNA is then ligated into the supplied linearized and dephosphorylated pWEB-TNCTM or pWEBTM Cosmid Vector, packaged using ultra-high efficiency MaxPlaxTM Lambda Packaging Extracts (,109 pfu/μg for phage lambda) and plated on phage T1-resistant EPI100TM-T1R E. coli plating cells, all included in the kit. The result is a complete and unbiased primary cosmid library ...
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From a cosmid gene bank of Bacillus cereus GP4 in Escherichia coli we isolated clones which, after several days of incubation, formed hemolysis zones on erythrocyte agar plates. These clones contained recombinant cosmids with B. cereus DNA insertions of varying lengths which shared some common restriction fragments. The smallest insertionwas recloned as aPstl fragment into pJKK3-1, a shuttle vector which repücates in Bacillus subtilis and E. coli. When this recombinant plasmid (pJKK3-1 hly-1) was transformed into E. coli, it caused hemolysis on erythrocyte agar plates, but in liquid assays no extemal or intemal hemolytic activity could be detected with the E. coli transformants. B. subtilis carrying the same plasmid exhibited hemolytic activity at Ievels comparable to those ofthe B. cereus donor strain. The hemolysin produced in B. subtilis seemed to be indistinguishable from cereolysin in its sensitivity to cholesterol, activation by dithiothreitol, and inactivation by antibodies raised ...
Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. coli) can only contain one fosmid molecule. Fosmids can hold DNA inserts of up to 40 kb in size; often the source of the insert is random genomic DNA. A fosmid library is prepared by extracting the genomic DNA from the target organism and cloning it into the fosmid vector. The ligation mix is then packaged into phage particles and the DNA is transfected into the bacterial host. Bacterial clones propagate the fosmid library. The low copy number offers higher stability than vectors with relatively higher copy numbers, including cosmids. Fosmids may be useful for constructing stable libraries from complex genomes. Fosmids have high structural stability and have been found to maintain human DNA effectively even after 100 generations of bacterial growth. Fosmid clones were used to help assess the accuracy of the Public Human Genome Sequence. The fertility plasmid or F-plasmid ...
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Depending on the particular aim of the experiment, broad host range cosmids, shuttle cosmids or 'mammalian' cosmids (linked to ... Cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. Cosmids can contain 37 ... Selection against wild type cosmid DNA is simply done via size exclusion. Cosmids, therefore, always form colonies and not ... is held by the terminase after the previous cosmid has been packaged, to prevent degradation by cellular DNases. Cosmids are ...
Examples are cosmids and phagemids. Transposons: These are DNA sequences that can move and replicate in different parts of a ...
... ; occipitotemporal sulcus (OTS); collateral sulcus (CoS); mid-fusiform sulcus (MFS). The fusiform gyrus can be ...
cosmid cpDNA See chloroplast DNA. CpG site A sequence of DNA in which a cytosine nucleotide is immediately followed by a ...
cosmid cpDNA See chloroplast DNA. CpG site A sequence of DNA in which a cytosine nucleotide is immediately followed by a ...
Once inside the host, the cosmids circularize with the aid of the host's DNA ligase and then function as plasmids. Cosmids are ... This sequence allows the cosmid to be packaged into bacteriophage λ particles. These particles- containing a linearized cosmid ... Cosmid vectors are plasmids that contain a small region of bacteriophage λ DNA called the cos sequence. ...
Alu repeats and polymorphisms in cosmids sequenced from chromosome 4p16.3". Nature Genetics. 1 (5): 348-53. doi:10.1038/ng0892- ...
Kim UJ, Shizuya H, de Jong PJ, Birren B, Simon MI (March 1992). "Stable propagation of cosmid sized human DNA inserts in an F ... Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. ... The major advantage of Fosmids over other cosmid systems lies in its capability of stably propagating human DNA fragments. ... The low copy number offers higher stability than vectors with relatively higher copy numbers, including cosmids. Fosmids may be ...
In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry ... The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes. Of these, the most commonly ...
... indicating it is on the cosmid 'F38H4', and there are at least 6 other genes on that cosmid. If a gene produces a protein that ... For example, in 2005 a 39 kb cosmid had to be inverted. Other improvements have come from comparing genomic DNA to cDNA ... These species therefore do not have sequence names for CDSs and gene transcripts that are based on cosmid names. Instead they ... which is derived from the cosmid, fosmid or YAC clone on which they reside, for instance F38H4.7, ...
1982). "Isolation of human oncogene sequences (v-fes homolog) from a cosmid library". Science. 216 (4550): 1136-8. Bibcode: ...
It is encoded by the P87050 2247 bp ORF on the cosmid 57A10. The protein is 775 amino acids in length. Cdr2 is a member of the ...
"Physical mapping of the mitochondrial genome of Arabidopsis thaliana by cosmid and YAC clones". Plant Journal. 6 (3): 447-455. ...
A cosmid library (31 249 bp in total) has been made from the genome. The estimated gene density based on the cosmid library is ...
1990). "High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones". Science. 247 (4938): 64-9 ...
June 1994). "Isolation of cosmid and cDNA clones in the region surrounding the BTK gene at Xq21.3-q22". Genomics. 21 (3): 517- ...
The location of frq was verified in 1986 through transformation of cosmids into frq9 and by rescuing the circadian rhythm. frq ...
1994). "Isolation of cosmid and cDNA clones in the region surrounding the BTK gene at Xq21.3-q22". Genomics. 21 (3): 517-24. ...
... a 1 Mbp cosmid and PAC contig". Genes, Chromosomes & Cancer. 31 (3): 228-39. doi:10.1002/gcc.1139. PMID 11391793. S2CID ...
1999). "A 500-kb sequence-ready cosmid contig and transcript map of the MEN1 region on 11q13". Genomics. 55 (1): 49-56. doi: ...
Cosmid/BAC/YAC end sequences use Cosmid/Bacterial artificial chromosome/Yeast artificial chromosome to sequence the genome from ... Cosmid/BAC/YAC can also be used to get bigger clone of DNA fragment than vectors like plasmid and phagemid. A larger insert is ... BAC can't do that, but BACs can reliably represent human DNA much better than YAC or cosmid. Exon trapped sequence is used to ... DDBJ-GSS MEGA- and GIGA preps of cosmid-, BAC-, PAC, YAC-, and P1-DNA with JETSTAR 2.0 "WSSP-04 Chapter 2 - Vectors" (PDF). ...
1994). "Isolation of cosmid and cDNA clones in the region surrounding the BTK gene at Xq21.3-q22". Genomics. 21 (3): 517-24. ...
The sequence was obtained by a combined approach, employing automated DNA sequence analysis of selected cosmids and whole- ...
... cytogenetic band location of 540 cosmids and 70 genes or DNA markers". Genomics. 15 (1): 133-45. doi:10.1006/geno.1993.1021. ...
January 1993). "Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and ...
... cytogenetic band location of 540 cosmids and 70 genes or DNA markers". Genomics. 15 (1): 133-45. doi:10.1006/geno.1993.1021. ...
January 1993). "Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and ...
... cytogenetic band location of 540 cosmids and 70 genes or DNA markers". Genomics. 15 (1): 133-45. doi:10.1006/geno.1993.1021. ...
... cytogenetic band location of 540 cosmids and 70 genes or DNA markers". Genomics. 15 (1): 133-145. doi:10.1006/geno.1993.1021. ...
January 1993). "Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and ...
Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility ... Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility ... Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility ... Southern analysis suggested a single copy of the cosmid had integrated into each of the transformants. Both of the independent ...
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An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region. In: Genomics. 1996 ; Vol. 32, No. 3. pp. 375 ... An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region. Genomics. 1996 Mar 15;32(3):375-387. doi: ... Dive into the research topics of An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region. Together ... An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region. / Osoegawa, Kazutoyo; Susukida, Rie; Okano, ...
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cosmid. phasmid. transposable element. other, please specify. (b) Identity of the vector ...
The COSMID study suggests that gastric bypass…offers a powerful treatment option for Asian Indians with type 2 diabetes, who ... In COSMID, 49% of the patients had a BMI of 25 to 30; 36% had a BMI 30 to 35, and only 15% had a BMI of 35 to 40. ... The COSMID population reflects the diabetic population in India, where people develop type 2 diabetes at a lower BMI and have a ... COSMID was funded by Ethicon, a subsidiary of Johnson & Johnson. Dr Shah is a consultant for Ethicon; disclosures for the ...
Chromosome-19 specific hncDNA clones were hybridized to chromosome 19 cosmids that were previously assembled into contigs ...
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For purification of up to 10 mg transfection-grade plasmid or cosmid DNA ... High-copy, low-copy, cosmid DNA. High-copy, low-copy, cosmid DNA. High-copy, low-copy, cosmid DNA. High-copy, low-copy, cosmid ... Parameters for purification of very low-copy plasmids and cosmids of less than 10 copies per cell ... High-copy, low-copy, cosmid DNA. Processing. Manual (gravity flow). Manual (gravity flow). Manual (gravity flow). Manual ( ...
BACs, Cosmids, Fosmids. 1μg (maximum). 20 picomoles = 1ul of 20uM primer. ...
These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine ...
High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones.. *P. Lichter, C. Tang, +4 authors. ... producing high-resolution maps of human chromosomes by in situ hybridization and show that by hybridizing three or more cosmids ...
Cytoscape node attribute (NOA) file translating cosmid names to 3-letter gene names. (NOA 10 kb) ...
The London Regional Genomics Centre is a Robarts Research Institute and Schulich School of Medicine & Dentistry led initiative to provide Next Generation Sequencing and Bioinformatics services on a fee-for-service/cost recovery basis.. ...
USTAN produced cosmid libraries, detected 9 gene clusters and a series of compounds;. • HZI improved the efficiency of ...
... cosmids C11D2.6 and C27F2.5; and K. Hamming and T. P. Snutch, unpublished results). ...
Ish-Horowicz D., Burke J. F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989-2998 ...
Chromosome-specific cosmid libraries have been prepared for 8 of the 13 chromosomes. Both commercially prepared cDNA libraries ...
Partial genome sequencing and screening of a cosmid DNA library resulted in the identification of a 1,302-bp... ...
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exon 45 cosmid probe (red) shows presence of donor-derived MSCs. (. f. and g. ) Simultaneous hybridization of DMD-BMT1 MSC ...
Isolation of large numbers of chromosome 3-specific cosmids containing clusters of rare restriction-endonuclease sites. ...
... or cosmid DNA. The expression elements of vectors vary in their strengths and specificities. Depending on the host-vector ...
  • Chromosome-19 specific hncDNA clones were hybridized to chromosome 19 cosmids that were previously assembled into contigs covering about 80% of Chr19. (nih.gov)
  • One hundred cosmid clones, each with 40-Kb DNA recognized fungal infection of hibernating bats. (cdc.gov)
  • These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones and are highly efficient for routine cloning applications. (sigmaaldrich.com)
  • High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. (semanticscholar.org)
  • A molecular map of MHC associated complement genes and steroid 21 hydroxylase loci based on EcoRI restriction pattern analysis of clones from a male sheep cosmid library. (biomedcentral.com)
  • This study requires a combination of chromosome painting and fluorescent in situ hybridization (FISH) with yeast artificial chromosomes (YAC) and cosmids. (medscape.com)
  • NEW ORLEANS - In the Comparison of Surgery vs Medicine for Indian Diabetes (COSMID ) study of Asian Indians with type 2 diabetes who were mainly below guideline-recommended body mass index (BMI) levels for bariatric surgery, Roux-en-Y gastric bypass led to diabetes remission more than half of the time. (medscape.com)
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  • The trial randomized 80 Asian Indians with type 2 diabetes who mostly had a BMI of 25 to 35 kg/m 2 to receive Roux-en-Y gastric bypass surgery or an intensive lifestyle-and-medication intervention. (medscape.com)
  • The results demonstrate the feasibility of rapidly producing high-resolution maps of human chromosomes by in situ hybridization and show that by hybridizing three or more cosmids simultaneously, gene order on the chromosome could be established unequivocally. (semanticscholar.org)
  • We present here a high- resolution physical map with megabase-sized cosmid/PAC contigs. (elsevier.com)
  • Rubin's group takes cosmids containing the gene, inserts them into Escherichia coli , and then mutagenizes them, showing that the gene can be inactivated. (the-scientist.com)
  • Chromosome-specific cosmid libraries have been prepared for 8 of the 13 chromosomes. (broadinstitute.org)
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  • Shashank S Shah, MD, a bariatric surgeon at the Laparo Obeso Centre in Pune, India, presented the findings from COSMID at the American Diabetes Association (ADA) 2016 Scientific Sessions last week, where he was awarded ADA's 2016 Vivian Fonseca Scholar Award for this research involving minorities. (medscape.com)
  • The COSMID population reflects the diabetic population in India, where people develop type 2 diabetes at a lower BMI and have a larger deposit of visceral fat than people in Western societies, according to Dr Shah. (medscape.com)
  • Southern analysis suggested a single copy of the cosmid had integrated into each of the transformants. (ejbiotechnology.info)
  • e ) FISH analysis using a dystrophin exon 45 cosmid probe (red) shows presence of donor-derived MSCs. (jci.org)
  • 6 ). A cosmid DNA library was constructed destructans genotype. (cdc.gov)
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  • Introduction of a cosmid carrying the wild-type chv region restored attachment and virulence and restored extracellular glucan production to chvA mutant A2505. (apsnet.org)
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  • The others are all cosmid vectors which allow insertion of up to 46 kb of plant genomic DNA, and which also contain all of the necessary sequences for A. tumefaciens-mediated plant transformation. (caltech.edu)
  • The cosmid vectors either contain a Hph marker (pCIT03), or a kanamycin-resistance marker (pCIT101-104). (caltech.edu)
  • Three of the cosmid vectors (pCIT30, pCIT101, and pCIT103) carry bacteriophage T7 and SP6 promoters flanking the cloning Bg/II site, for synthesis of end-specific RNAs. (caltech.edu)
  • The other two cosmid vectors (pCIT102 and pCIT104) carry restriction sites flanking the insertion site (XhoI) for convenient release of the insert by restriction digests. (caltech.edu)
  • Isolation of clonable DNA (genomic libraries, cDNA synthesis) cloning vectors (plasmids, bacteriophages, cosmids) plasmid incompatibility and control of copy number. (up.ac.za)
  • The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes. (agscientific.com)
  • A DNA library is a There are genomic DNA libraries which contain a representation of an organism https://en.m.wikipedia.org/wiki/CDNA_library A DNA library is a collection of DNA fragments that have been cloned into vectors so that researchers can identify and isolate the DNA fragments that interest them for further study. (northshoregreenbuild.com)
  • We report the complete sequence of two cosmids, SPBC19C7 (34815 bp insert, Accession No. AL023859) and SPBC15D4 (33203 bp insert, Accession No. AL031349), localized on chromosome II of the S. pombe genome. (pasteur.fr)
  • A physical map of the genome has been assembled with a combination of cosmid and YAC genes. (cshlpress.com)
  • Cloning of the complete human cytomegalovirus genome in cosmids. (microbiologyresearch.org)
  • I developed a system to produce mutations in the Epstein-Barr virus (EBV) genome by transfecting cells with cosmid DNAs to obtain recombinant viruses. (nih.gov)
  • We constructed a set of four cosmids that encompass the 125-kb VZV genome. (nih.gov)
  • In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g.-plasmid, cosmid, Lambda phages). (agscientific.com)
  • In vitro packaging of lambda and cosmid DNA. (wikidata.org)
  • Micronuclei obtained from peripheral blood lymphocytes by microdissection were subjected to random amplification of polymorphic DNA (RAPD-PCR), and a unique PCR product was then used to isolate a cosmid clone from a human genomic library. (nih.gov)
  • A Tn7-like element was found in a region downstream of a cosmid (p818.1) isolated from a genomic library of Thiobacillus ferrooxidans ATCC 33020. (uct.ac.za)
  • Rapid and efficient cosmid cloning. (wikidata.org)
  • The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. (qiagen.com)
  • The QIAprep Spin Miniprep Kit enables purification of up to 20 μg molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as PCR, sequencing and cloning. (qiagen.com)
  • Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. (academicinfluence.com)
  • A cosmid clone carrying 16 kilobases (kb) of contiguous genomic. (oregonstate.edu)
  • 4] "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV reveals 12 new open reading frames (ORFs) and an ancient duplication of six ORFs. (tcdb.org)
  • We have therefore identified, in a region consistently deleted in a variety of lung cancers, two cosmids that may contain genes that are candidates for involvement in lung cancer. (elsevier.com)
  • Two known genes were found on each cosmid: cyr1 and uve1 on SPBC19C7, encoding adenylate cyclase and a UV-endonuclease, respectively, and gpt and pho2 on SPBC15D4, encoding an N-acetylglucosamine-1-phosphate transferase and a4-nitrophenylphosphatase, respectively. (pasteur.fr)
  • That one CD1 + cosmid encodes two pCD1 genes demonstrates that pig CD1 genes, like human, are closely linked. (elsevier.com)
  • We have isolated large numbers of human recombinants from a cosmid library constructed from an interspecific (hamster/human )somatic cell hybrid whose only human component is an intact chromosome 3. (elsevier.com)
  • Buroker NE, Magenis RE, Weliky K, Bruns G, Litt M , Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. (coriell.org)
  • Bufton L, Bruns GA, Magenis RE, Tomar D, Shaw D, Brook D, Litt M , Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to chromosome 19. (coriell.org)
  • We were building YAC [yeast artificial chromosome] libraries, cosmid libraries. (nih.gov)
  • Two unpublished cases with partial tandem duplication of 12p and one previously published case were studied by fluorescence in situ hybridization using 11 cosmid DNA probes from 12p. (nih.gov)
  • A new high-capacity cosmid vector and its use. (wikidata.org)
  • A cosmid vector that facilitates restriction enzyme mapping. (wikidata.org)
  • The transforming DNA was molecularly cloned by using cosmid vector pJB8 to determine its structure and origin. (elsevier.com)
  • O ADN xenómico para ERCC1 foi o primeiro xene de reparación do ADN humano en ser illado por clonación molecular. (wikipedia.org)
  • Bio-Rad offers Certified™ Megabase Agarose, Certified Pulsed-Field Agarose, and standards for applications from FIGE separation of cosmid inserts to separation of the largest chromosomes. (bio-rad.com)
  • We identified two cosmids, cA84 (D3S92) and cA199 (D3S93), derived from within chromosomal band 3p21.1. (elsevier.com)
  • A probe made from the Tn7-like element hybridized to restriction fragments of identical size from both cosmid p818.1 and T ferrooxidans chromosomal DNA. (uct.ac.za)
  • Both cosmids contained multiple rare restriction sites that were tightly clustered within the cosmids. (elsevier.com)
  • An 11·5 kb Eco RI fragment was isolated from a cosmid bank of G. alpicola and was shown to complement a recA deletion in Escherichia coli S. 17 and JC10289. (microbiologyresearch.org)
  • It is ideal for many applications, including Northern and Southern blotting, Cosmid library restriction analysis, STS screening, Microsatellite analysis, PCR fragment analysis, RFLP analysis, DNA fingerprinting and high-throughput analysis. (labiol.pl)
  • We have developed a novel method for screening a Mycobacterium bovis (BCG) cosmid library in Mycobacterium smegmatis for the detection of immunostimulatory T-cell antigens (Ag). (elsevier.com)
  • Prepacked QIAGEN-tips (see figure " Anion-exchange tips. For purification of up to 10 mg transfection-grade plasmid or cosmid DNA "> Anion-exchange tips ") operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. (qiagen.com)
  • Transfection of cells in culture with these four cosmids results in recombination and production of infectious VZV. (nih.gov)
  • Tg19959 mice were constructed by injecting FVB x 129S6 F1 embryos with a cosmid insert containing human APP695 with two familial AD mutations (KM670/671NL and V717F), under the control of the hamster PrP promoter. (nih.gov)
  • NEW ORLEANS - In the Comparison of Surgery vs Medicine for Indian Diabetes (COSMID ) study of Asian Indians with type 2 diabetes who were mainly below guideline-recommended body mass index (BMI) levels for bariatric surgery, Roux-en-Y gastric bypass led to diabetes remission more than half of the time. (medscape.com)
  • The trial randomized 80 Asian Indians with type 2 diabetes who mostly had a BMI of 25 to 35 kg/m 2 to receive Roux-en-Y gastric bypass surgery or an intensive lifestyle-and-medication intervention. (medscape.com)
  • Whereas SOX9 maps to 17q, a SOX10 cosmid has previously been mapped by us to the region 22q13.1. (uni.lu)
  • Cara Ruby (who goes by Cara Boyle) has some of the best candid sets on Cosmid. (demokratst.ru)
  • Visit Cosmid for complete sets, poster-size definition, hotter action and videos! (hqbabes.com)
  • The Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wx allele of Zea mays. (wikidata.org)
  • Shashank S Shah, MD, a bariatric surgeon at the Laparo Obeso Centre in Pune, India, presented the findings from COSMID at the American Diabetes Association (ADA) 2016 Scientific Sessions last week, where he was awarded ADA's 2016 Vivian Fonseca Scholar Award for this research involving minorities. (medscape.com)