Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.
Semi-synthetic derivative of penicillin that functions as an orally active broad-spectrum antibiotic.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Elements of limited time intervals, contributing to particular results or situations.
Nonsusceptibility of a microbe to the action of ampicillin, a penicillin derivative that interferes with cell wall synthesis.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A live VACCINIA VIRUS vaccine of calf lymph or chick embryo origin, used for immunization against smallpox. It is now recommended only for laboratory workers exposed to smallpox virus. Certain countries continue to vaccinate those in the military service. Complications that result from smallpox vaccination include vaccinia, secondary bacterial infections, and encephalomyelitis. (Dorland, 28th ed)
An acute, highly contagious, often fatal infectious disease caused by an orthopoxvirus characterized by a biphasic febrile course and distinctive progressive skin eruptions. Vaccination has succeeded in eradicating smallpox worldwide. (Dorland, 28th ed)
Hostile conflict between organized groups of people.
A disorder characterized by recurrent episodes of paroxysmal brain dysfunction due to a sudden, disorderly, and excessive neuronal discharge. Epilepsy classification systems are generally based upon: (1) clinical features of the seizure episodes (e.g., motor seizure), (2) etiology (e.g., post-traumatic), (3) anatomic site of seizure origin (e.g., frontal lobe seizure), (4) tendency to spread to other structures in the brain, and (5) temporal patterns (e.g., nocturnal epilepsy). (From Adams et al., Principles of Neurology, 6th ed, p313)
The type species of ORTHOPOXVIRUS, related to COWPOX VIRUS, but whose true origin is unknown. It has been used as a live vaccine against SMALLPOX. It is also used as a vector for inserting foreign DNA into animals. Rabbitpox virus is a subspecies of VACCINIA VIRUS.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
Management control systems for structuring health care delivery strategies around case types, as in DRGs, or specific clinical services.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Research that involves the application of the natural sciences, especially biology and physiology, to medicine.
Ordered compilations of item descriptions and sufficient information to afford access to them.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development.
Higher plants that live primarily in terrestrial habitats, although some are secondarily aquatic. Most obtain their energy from PHOTOSYNTHESIS. They comprise the vascular and non-vascular plants.
A monophyletic group of green plants that includes all land plants (EMBRYOPHYTA) and all green algae (CHLOROPHYTA and STREPTOPHYTA).
Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.
Mapping of the linear order of genes on a chromosome with units indicating their distances by using methods other than genetic recombination. These methods include nucleotide sequencing, overlapping deletions in polytene chromosomes, and electron micrography of heteroduplex DNA. (From King & Stansfield, A Dictionary of Genetics, 5th ed)
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
A congenital cardiovascular malformation in which the AORTA arises entirely from the RIGHT VENTRICLE, and the PULMONARY ARTERY arises from the LEFT VENTRICLE. Consequently, the pulmonary and the systemic circulations are parallel and not sequential, so that the venous return from the peripheral circulation is re-circulated by the right ventricle via aorta to the systemic circulation without being oxygenated in the lungs. This is a potentially lethal form of heart disease in newborns and infants.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
Educational institutions.
Common name for one of five species of small PARROTS, containing long tails.
An order of fungi in the phylum ASCOMYCOTA that are important plant pathogens.
Instruments used for injecting or withdrawing fluids. (Stedman, 25th ed)
Organized efforts to insure obedience to the laws of a community.
A complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.
Bacteria which lose crystal violet stain but are stained pink when treated by Gram's method.
Bacteria which retain the crystal violet stain when treated by Gram's method.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Substances that reduce the growth or reproduction of BACTERIA.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Aminoglycoside produced by Streptomyces hygroscopicus. It is used as an anthelmintic against swine infections by large roundworms, nodular worms, and whipworms.
PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.
A plant genus of the family CAPPARACEAE that contains cleogynol and 15alpha-acetoxycleomblynol (dammaranes) and 1-epibrachyacarpone (a triterpene), and ISOTHIOCYANATES.

Analysis of two cosmid clones from chromosome 4 of Drosophila melanogaster reveals two new genes amid an unusual arrangement of repeated sequences. (1/1067)

Chromosome 4 from Drosophila melanogaster has several unusual features that distinguish it from the other chromosomes. These include a diffuse appearance in salivary gland polytene chromosomes, an absence of recombination, and the variegated expression of P-element transgenes. As part of a larger project to understand these properties, we are assembling a physical map of this chromosome. Here we report the sequence of two cosmids representing approximately 5% of the polytenized region. Both cosmid clones contain numerous repeated DNA sequences, as identified by cross hybridization with labeled genomic DNA, BLAST searches, and dot matrix analysis, which are positioned between and within the transcribed sequences. The repetitive sequences include three copies of the mobile element Hoppel, one copy of the mobile element HB, and 18 DINE repeats. DINE is a novel, short repeated sequence dispersed throughout both cosmid sequences. One cosmid includes the previously described cubitus interruptus (ci) gene and two new genes: that a gene with a predicted amino acid sequence similar to ribosomal protein S3a which is consistent with the Minute(4)101 locus thought to be in the region, and a novel member of the protein family that includes plexin and met-hepatocyte growth factor receptor. The other cosmid contains only the two short 5'-most exons from the zinc-finger-homolog-2 (zfh-2) gene. This is the first extensive sequence analysis of noncoding DNA from chromosome 4. The distribution of the various repeats suggests its organization is similar to the beta-heterochromatic regions near the base of the major chromosome arms. Such a pattern may account for the diffuse banding of the polytene chromosome 4 and the variegation of many P-element transgenes on the chromosome.  (+info)

Nonmethylated transposable elements and methylated genes in a chordate genome. (2/1067)

The genome of the invertebrate chordate Ciona intestinalis was found to be a stable mosaic of methylated and nonmethylated domains. Multiple copies of an apparently active long terminal repeat retrotransposon and a long interspersed element are nonmethylated and a large fraction of abundant short interspersed elements are also methylation free. Genes, by contrast, are predominantly methylated. These data are incompatible with the genome defense model, which proposes that DNA methylation in animals is primarily targeted to endogenous transposable elements. Cytosine methylation in this urochordate may be preferentially directed to genes.  (+info)

Three receptor genes for plasminogen related growth factors in the genome of the puffer fish Fugu rubripes. (3/1067)

Plasminogen related growth factors (PRGFs) and their receptors play major roles in embryogenesis, tissue regeneration and neoplasia. In order to investigate the complexity and evolution of the PRGF receptor family we have cloned and sequenced three receptors for PRGFs in the teleost fish Fugu rubripes, a model vertebrate with a compact genome. One of the receptor genes isolated encodes the orthologue of mammalian MET, whilst the other two may represent Fugu rubripes orthologues of RON and SEA. This is the first time three PRGF receptors have been identified in a single species.  (+info)

Complete exon-intron organization of the mouse fibulin-1 gene and its comparison with the human fibulin-1 gene. (4/1067)

Fibulin-1 is a 90 kDa calcium-binding protein present in the extracellular matrix and in the blood. Two major variants, C and D, differ in their C-termini as well as the ability to bind the basement membrane protein nidogen. Here we characterized genomic clones encoding the mouse fibulin-1 gene, which contains 18 exons spanning at least 75 kb of DNA. The two variants are generated by alternative splicing of exons in the 3' end. By searching the database we identified most of the exons encoding the human fibulin-1 gene and showed that its exon-intron organization is similar to that of the mouse gene.  (+info)

Genetic variation of chlorella viruses: variable regions localized on the CVK2 genomic DNA. (5/1067)

A physical map of the Chlorella virus CVK2 genomic DNA has been constructed based on a cosmid contig covering the entire genomic region. By using Southern blot analysis with 22 gene probes, the gene arrangement along the genome was compared between CVK2 and PBCV-1, the prototypic member of Phycodnaviridae, whose genomic sequence is now available. The major rearrangements were (1) an insertion of a 20-kbp region around the left end of CVK2 DNA, (2) a duplication of the gene for major capsid protein in CVK2 DNA, (3) deletions/insertions of some open reading frames, and (4) divergence in the terminal inverted repeat sequences. Despite these changes, extensive colinearity was revealed between most of the genes along the CVK2 and PBCV-1 genomes. These data imply that the Chlorella virus genome has an overall high degree of genomic stability, encompassing specific islands of rearrangements.  (+info)

Comparative genomic analysis of the interferon/interleukin-10 receptor gene cluster. (6/1067)

Interferons and interleukin-10 are involved in key aspects of the host defence mechanisms. Human chromosome 21 harbors the interferon/interleukin-10 receptor gene cluster linked to the GART gene. This cluster includes both components of the interferon alpha/beta-receptor (IFNAR1 and IFNAR2) and the second components of the interferon gamma-receptor (IFNGR2) and of the IL-10 receptor (IL10R2). We report here the complete gene content of this GART-cytokine receptor gene cluster and the use of comparative genomic analysis to identify chicken IFNAR1, IFNAR2, and IL10R2. We show that the large-scale structure of this locus is conserved in human and chicken but not in the pufferfish Fugu rubripes. This establishes that the receptor components of these host defense mechanisms were fixed in an ancestor of the amniotes. The extraordinary diversification of the interferon ligand family during the evolution of birds and mammals has therefore occurred in the context of a fixed receptor structure.  (+info)

Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication. (7/1067)

Human DNA topoisomerase II is essential for chromosome segregation and is the target for several clinically important anticancer agents. It is expressed as genetically distinct alpha and beta isoforms encoded by the TOP2alpha and TOP2beta genes that map to chromosomes 17q21-22 and 3p24, respectively. The genes display different patterns of cell cycle- and tissue-specific expression, with the alpha isoform markedly upregulated in proliferating cells. In addition to the fundamental role of TOP2alpha and TOP2beta genes in cell growth and development, altered expression and rearrangement of both genes are implicated in anticancer drug resistance. Here, we report the complete structure of the human topoisomerase IIalpha gene, which consists of 35 exons spanning 27.5 kb. Sequence data for the exon-intron boundaries were determined and examined in the context of topoisomerase IIalpha protein structure comprising three functional domains associated with energy transduction, DNA breakage-reunion activity and nuclear localization. The organization of the 3' half of human TOP2beta, including sequence specifying the C-terminal nuclear localization domain, was also elucidated. Of the 15 introns identified in this 20 kb region of TOP2beta, the first nine and the last intron align in identical positions and display the same phases as introns in TOP2alpha. Though their extreme 3' ends differ, the striking conservation suggests the two genes diverged recently in evolutionary terms consistent with a gene duplication event. Access to TOP2alpha and TOP2beta gene structures should aid studies of mutations and gene rearrangements associated with anticancer drug resistance.  (+info)

Control of fertilization-independent endosperm development by the MEDEA polycomb gene in Arabidopsis. (8/1067)

Higher plant reproduction is unique because two cells are fertilized in the haploid female gametophyte. Egg and sperm nuclei fuse to form the embryo. A second sperm nucleus fuses with the central cell nucleus that replicates to generate the endosperm, a tissue that supports embryo development. To understand mechanisms that initiate reproduction, we isolated a mutation in Arabidopsis, f644, that allows for replication of the central cell and subsequent endosperm development without fertilization. When mutant f644 egg and central cells are fertilized by wild-type sperm, embryo development is inhibited, and endosperm is overproduced. By using a map-based strategy, we cloned and sequenced the F644 gene and showed that it encodes a SET-domain polycomb protein. Subsequently, we found that F644 is identical to MEDEA (MEA), a gene whose maternal-derived allele is required for embryogenesis [Grossniklaus, U., Vielle-Calzada, J.-P., Hoeppner, M. A. & Gagliano, W. B. (1998) Science 280, 446-450]. Together, these results reveal functions for plant polycomb proteins in the suppression of central cell proliferation and endosperm development. We discuss models to explain how polycomb proteins function to suppress endosperm and promote embryo development.  (+info)

Yeah you could use the simple protocl for transformation like one given in manniatis involving cacl2 treated competent cells. I have used this method for transforming 21 kb cosmid. I hope this helps ...
TY - JOUR. T1 - A YAC-, P1-, and cosmid-based physical Map of the BRCA1 region on chromosome 17q21. AU - Couch, Fergus J.. AU - Castilla, Lucio H.. AU - Xu, Junzhe. AU - Abel, Kenneth J.. AU - Welcsh, Piri. AU - King, Stephanie E.. AU - Wong, Linghua. AU - Ho, Peggy P.. AU - Merajver, Sofia. AU - Brody, Lawrence C.. AU - Yin, Guiying. AU - Hayes, Steve T.. AU - Gieser, Linn M.. AU - Flejter, Wendy L.. AU - Glover, Thomas W.. AU - Friedman, Lori S.. AU - Lynch, Eric D.. AU - Meza, Jose E.. AU - King, Mary Claire. AU - Law, David J.. AU - Deaven, Larry. AU - Bowcock, Anne M.. AU - Collins, Francis S.. AU - Weber, Barbara L.. AU - Chandrasekharappa, Settara C.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - A familial early-onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. To characterize this region and to aid in the identification of the BRCA1 gene, a physical map of a region of 1.0-1.5 Mb between the EDH17B1 and the PPY loci on chromosome 17q21 was generated. The physical map is ...
The set of clone ends is dumped as part of the gff files: http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE/GFF_files.shtml This is the source for the extents displayed in WormBase. The caveat with this is that the true end is not marked up for all clones. The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left and right ends as this became part of the standard procedure. Finally, many of the YACs do not have clone ends because the segment submitted to GenBank/EMBL is much smaller than the full clone, and hence the true ends lie within sequences already finished at that stage of the sequencing (i.e. we never went back to update clone ends in sequence already finished ...
The set of clone ends is dumped as part of the gff files: http://www.sanger.ac.uk/Projects/C_elegans/WORMBASE/GFF_files.shtml This is the source for the extents displayed in WormBase. The caveat with this is that the true end is not marked up for all clones. The early cosmids do not have such annotations because nobody thought about marking them up. Later cosmids do have clone left and right ends as this became part of the standard procedure. Finally, many of the YACs do not have clone ends because the segment submitted to GenBank/EMBL is much smaller than the full clone, and hence the true ends lie within sequences already finished at that stage of the sequencing (i.e. we never went back to update clone ends in sequence already finished ...
A few weeks ago I described some problems we were having with cosmid libraries. I got several good responses. The one below was the best and might be useful to others. What you have been seeing is actually the gradual loss of the selection pressure, which results from the degradation of the ampicillin in the medium by the beta-lactamase produced by the amp- containing clone. This eventually leads to the loss of the amp- containing cosmid. You might recall the growth of satellite colonies around the genuine transformants when you let the incubation of transformation plates go longer, say two days instead of overnight. After 4-6 weeks the clone has lost the amp-containing cosmid and becomes ampicillin sensitive. That is why you transferred the cells to ampicillin-containing liquid medium and you did not see any growth the next day. The same goes for the lower yield of cosmid DNA after 3-4 weeks, since the colony contains a population of resistant and sensitive cells. My suggestion to you is to ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
pWEB::TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies,The pWEB::TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of genomic DNA for subsequent use in making nested deletion sublibraries. High efficiency cosmid library construction is accomplished using easy and highly repr,biological,biology supply,biology supplies,biology product
10 mg transfection-grade endofree plasmid or cosmid DNA, HiSpeed Mega, HiSpeed Giga, EF plasmid, HiSpeed Mega and Giga EF Plasmid Kits
The DHFR-G8 cell line was produced by M.C. Hung, et al., in 1986 from the NIH/3T3 mouse fibroblast cell line which was cotransfected with the cNEU-p clone and the pSV2-DHFR plasmid. This cell line was developed from one methotrexate resistant colony by selection in 0.6 uM methotrexate and 10% dialyzed calf serum. These cells contain 50-100 copies of the cNeu-P (normal rat cosmid DNA) and produce approximately 4 x 10(5) molecules of encoded p185 protein per cell.
SpinSmart Plasmid Miniprep kits are designed to rapidly purify plasmid DNA from bacterial cultures.The protocol below is appropriate for both CM-410-50 and CM-410-250.
Ji H, Smith L.M, Guilfoyle RA. 1994. Rapid isolation of cosmid insert DNA by triple-helix-mediated affinity capture. Genetic Analysis, Techniques and Applications. 11:43-7. ...
Ji H, Smith L.M, Guilfoyle RA. 1994. Rapid isolation of cosmid insert DNA by triple-helix-mediated affinity capture. Genetic Analysis, Techniques and Applications. 11:43-7. ...
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cosmid definition: Genetics A hybrid vector that has been spliced with plasmid DNA for cloning huge genes or gene fragments.; a kind of plasmid (often utilized as a cloning vector) constructed because…
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The method used for the isolation of large scale cosmid and plasmid DNA is an unpublished modification (16) of an alkaline lysis procedure (17,18) followed by equilibrium ultracentrifugation in cesium chloride-ethidium bromide gradients (1). Briefly, cells containing the desired plasmid or cosmid are harvested by centrifugation, incubated in a lysozyme buffer, and treated with alkaline detergent. Detergent solubilized proteins and membranes are precipitated with sodium acetate, and the lysate is cleared first by filtration of precipitate through cheesecloth and then by centrifugation. The DNA-containing supernatant is transferred to a new tube, and the plasmid or cosmid DNA is precipitated by the addition of polyethylene glycol and collected by centrifugation. The DNA pellet is resuspended in a buffer containing cesium chloride and ethidium bromide, which is loaded into polyallomer tubes and subjected to ultracentrifugation overnight. The ethidium bromide stained plasmid or cosmid DNA bands, ...
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The NucleoBond BAC 100 Kit is designed to purify large DNA fragments such as cosmids, bacteriophage P1 clones, PACs, and BACs, without phenol/chloroform extraction. 1 hour protocol accomodates vectors up to to 300 kb.
Maxiprep NucleoBond Plasmid Kits: Purify plasmid DNA, cosmids, BACs, PACs, and YACs in just a few hours-no phenol/chloroform extraction. Use for transfection, in vitro transcription, and automated sequencing.
Kanamycin - The frozen stock solutions of kanamycin are at 50mg/ml in H2O, and are marked with green. The final concentration for LB liquid culture for growing plasmids is 50ug/ml, and for cosmids is 20ug/ml. To obtain 50ng/ml in 100ml of LB, add 100ul stock solution, and to obtain 20ug/ml, add 40ul stock solution ...
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Despite screening various growth media, we failed to detect the production of any 2-hydroxyphenylthiazoline-containing metabolites by S. venezuelae. This is potentially explained by the low levels of sven0516 expression in the bldM mutant (Fig. 3), which is surprising given that sven0517 is likely to be in the same operon, and possibly reflects differential mRNA stability for the two genes. We therefore elected to express the sven0503-sven0517 gene cluster in the engineered host S. coelicolor M1152.23 A clone (SV-2_E03) from an ordered genomic cosmid library of the S. venezuelae chromosome containing a segment extending from sven0496 to sven0518 was PCR-targeted in Escherichia coli with a 5.2 kb SspI fragment from pIJ10702 that contains oriT, and the øC31 integrase gene and phage attachment site (attP). The resulting cosmid, SV-2_E03::SspI, was introduced into S. coelicolor M1152 by conjugation, whereupon it integrated into the chromosomal øC31 attB site. Wild type S. coelicolor M1152 and the ...
TY - JOUR. T1 - Isolation and characterization of a novel gene deleted in digeorge syndrome. AU - Kurahashi, Hiroki. AU - Akagi, Kenzo. AU - Inazawa, Johji. AU - Ohta, Tohru. AU - Niikawa, Norio. AU - Kayatani, Futoshi. AU - Sano, Tetsuya. AU - Okada, Shintaro. AU - Nishisho, Isamu. PY - 1995/4/1. Y1 - 1995/4/1. N2 - The region commonly deleted in DiGeorge syndrome (DGS) has been localized at 22q11.1-q11.2 with the aid of a high resolution banding technique. A 22q11 specific plasmid library was constructed with a microdissection and microcloning method. Dosage analysis proved three of 144 randomly selected microclones to detect hemizygosity in two patients with DGS. Two of the clones were found to contain independent low-copy-number repetitive sequences, all of which were included in the region deleted in the DGS patients. Screening of the cosmid library and subsequent cosmid walking allowed us to obtain two cosmid contigs corresponding to the microclones within the deletion (contig 1 and contig ...
8-base recognition sites will yield pieces 64,000 bases long. Since hundreds of different restriction enzymes have been characterized, DNA can be cut into many different small fragments. Physical Maps Different types of physical maps vary in their degree of resolution. The lowest- resolution physical map is the chromosomal (sometimes called cytogenetic) map, which is based on the distinctive banding patterns observed by light microscopy of stained chromosomes. A cDNA map shows the locations of expressed DNA regions (exons) on the chromosomal map. The more detailed cosmid contig map depicts the order of overlapping DNA fragments spanning the genome. A macrorestriction map describes the order and distance between enzyme cutting (cleavage) sites. The highest- resolution physical map is the complete elucidation of the DNA base- pair sequence of each chromosome in the human genome. Physical maps are described in greater detail below. Low-Resolution Physical Mapping Chromosomal map. In a chromosomal ...
Empirical Bioscience announced the introduction of a new Plasmid Mini-Prep Kit today. Requiring only 1-3mL of bacterial culture, the kit isolates high-quality plasma or cosmid DNA for extraction up to 10 kb in length and yields up to 20 µg per preparation.. In addition to using smaller volumes of cultures, the kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches the optimal pH level for DNA binding, 7.5. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation.. Besides the color changing lysis buffer, the kit includes, neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns and collection tubes. These components create a versatile kit that lends itself well for use in ...
The data section can follow or proceed the configuration section. The two sections can also be intermixed. The data section is a tab or whitespace-delimited file which you can export from a spreadsheet application or word processor file (be sure to save as text only!). Here is an example data section:. Cosmid B0511 . 516-619 Cosmid B0511 . 3185-3294 Cosmid B0511 . 10946-11208 Cosmid B0511 . 13126-13511 Cosmid B0511 . 66-208 Cosmid B0511 . 6354-6499 Cosmid B0511 . 13955-14115 EST yk595e6.5 + 3187-3294 EST yk846e07.3 - 11015-11208 EST yk53c10 yk53c10.5 + 18892-19154 yk53c10.3 - 15000-15500,15700-15800 EST yk53c10.5 + 16032-16105 SwissProt PECANEX + 13153-13656 Swedish fish FGENESH Gene 1 - 1-205,518-616,661-735,3187-3365,3436-3846 Transmembrane domain FGENESH Gene 2 - 16626-17396,17451-17597 Kinase and sushi domains. Each line of the file contains five columns. The columns are:. ...
Cosmid clones containing alpha 1-antitrypsin (alpha 1AT) gene sequences were observed to contain alpha 1AT-like sequences approximately 12 kb downstream of the authentic alpha 1AT gene. Restriction mapping suggested the alpha 1AT-like gene lacks promoter sequences. Cosmid clones from one library con …
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TY - JOUR. T1 - Detection of submicroscopic chromosomal deletions in aniridia patients using fluorescence in situ hybridization and a panel of cosmids covering the WT1 gene. AU - Kempski, H.. AU - Cowell, J. K.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - A series of cosmids have been isolated from a human chromosome 11-specific cosmid library using the human Wilms tumour predisposition gene cDNA, WT33. Seven overlapping cosmids were isolated which cover the genomic sequence of WT1 and in situ hybridisation shows that they all localise to the p13 region of chromosome 11. Chromosomes from patients with aniridia and Wilms tumour, and a small subband deletion in 11p13, were analysed and no hybridisation signal was seen on the deletion chromosomes. These cosmids, therefore, can be used to analyse chromosomes from patients with sporadic aniridia for submicroscopic deletions. Aniridia patients who show normal hybridisation patterns on both chromosomes need no longer be screened for Wilms tumours.. AB - A ...
The I-2 locus in tomato confers resistance to race 2 of the soil-borne fungus Fusarium oxysporum f sp lycopersici. The selective restriction fragment amplification (AFLP) positional cloning strategy was used to identify I-2 in the tomato genome. A yeast artificial chromosome (YAC) clone covering ~750 kb encompassing the I-2 locus was isolated, and the AFLP technique was used to derive tightly linked AFLP markers from this YAC clone. Genetic complementation analysis in transgenic R1 plants using a set of overlapping cosmids covering the I-2 locus revealed three cosmids giving full resistance to F. o. lycopersici race 2. These cosmids shared a 7-kb DNA fragment containing an open reading frame encoding a protein with similarity to the nucleotide binding site leucine-rich repeat family of resistance genes. At the I-2 locus, we identified six additional homologs that included the recently identified I-2C-1 and I-2C-2 genes. However, cosmids containing the I-2C-1 or I-2C-2 gene could not confer ...
Empirical Bioscience announced the introduction of a new Plasmid Mini-Prep Kit today. Requiring only 1-3mL of bacterial culture, the kit isolates high-quality plasma or cosmid DNA for extraction up to 10 kb in length and yields up to 20 µg per preparation. In addition to using smaller volumes of cultures, the kit also features an integrated pH indicator within the lysis buffer. This indicator changes color to bright yellow when the lysis buffer reaches the optimal pH level for DNA binding, 7.5. If the pH is greater than 7.5 the lysis buffer will appear orange or violet in color indicating that the pH level is inefficient for DNA adsorption, allowing researchers to make adjustments to the mixture for more efficient plasmid isolation. Besides the color changing lysis buffer, the kit includes, neutralization buffer, Rnase A, activation buffer, washing buffer, elution buffer, spin columns and collection tubes. These components create a versatile kit that lends itself well for use in ...
A series of 130 DNA probes, hybridized to the CIC YAC library, are ordered along the abscissa. A subset of 58 of the probes, mapped to chromosome 2 by RFLP analysis, serve as contact points between the genetic and physical maps. The positions of markers mapped to the recombinant inbred lines (7) are indicated in cM above the marker names. When a set of probes hybridized to the same YACs, their order is listed by their position in the genetic map (7). If no genetic or auxilliary data (e.g. cosmid contig (4)) was available, the exact probe order is indeterminate and is arbitrarily assigned.. A line drawn above a marker indicates a difference in marker order between the physical and genetic maps. Markers designated RI have been mapped by us to the recombinant inbred populations and are in correct relative position (with one exception, 15414, marked RI-?) to the markers on either side (no number is indicated as adding markers and using a different mapping program generates different absolute ...
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Brian Robertson wrote: , , The amount of bacterial genome data available as sequenced cosmids of , 30-40 kb is increasing rapidly. Our problem is that we need to keep track , of newly discovered genes as they appear, so they can be incorporated into , our research program as appropriate. For this we need to create lists of , probable genes identified in the annotations for each cosmid. This can , then be circulated to laboratory workers. , , An example of this kind of annotation is shown below. We would like to , extract the /note field, which contains the probable function of the , gene, and create a list of these for each cosmid. , , FT CDS_pept complement(3043..4155) , FT /note=MTCY190.03c, probable anthranilate , FT phosphoribosyltransferase, trpD, len: 370, similar to eg , FT SW:TRPD_LACCA P17170, (43.2% identity in 308 aa overlap), , FT initiation codon uncertain, gtg at 4086 favoured by , FT homology but this has no clear ribosome binding site , , Does anyone know of a way of ...
The kits utilize a novel strategy of cloning end-repaired, randomly sheared DNA instead of the conventional approach of cloning fragments generated by partial restriction endonuclease digestion. First, genomic DNA is sheared by passing it through a syringe needle. The sheared DNA is end-repaired to generate 5-phosphorylated blunt ends and size-selected using a low melting point agarose gel. The size-selected DNA is then ligated into the supplied linearized and dephosphorylated pWEB-TNCTM or pWEBTM Cosmid Vector, packaged using ultra-high efficiency MaxPlaxTM Lambda Packaging Extracts (,109 pfu/μg for phage lambda) and plated on phage T1-resistant EPI100TM-T1R E. coli plating cells, all included in the kit. The result is a complete and unbiased primary cosmid library ...
The kits utilize a novel strategy of cloning end-repaired, randomly sheared DNA instead of the conventional approach of cloning fragments generated by partial restriction endonuclease digestion. First, genomic DNA is sheared by passing it through a syringe needle. The sheared DNA is end-repaired to generate 5-phosphorylated blunt ends and size-selected using a low melting point agarose gel. The size-selected DNA is then ligated into the supplied linearized and dephosphorylated pWEB-TNCTM or pWEBTM Cosmid Vector, packaged using ultra-high efficiency MaxPlaxTM Lambda Packaging Extracts (,109 pfu/μg for phage lambda) and plated on phage T1-resistant EPI100TM-T1R E. coli plating cells, all included in the kit. The result is a complete and unbiased primary cosmid library ...
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From a cosmid gene bank of Bacillus cereus GP4 in Escherichia coli we isolated clones which, after several days of incubation, formed hemolysis zones on erythrocyte agar plates. These clones contained recombinant cosmids with B. cereus DNA insertions of varying lengths which shared some common restriction fragments. The smallest insertionwas recloned as aPstl fragment into pJKK3-1, a shuttle vector which repücates in Bacillus subtilis and E. coli. When this recombinant plasmid (pJKK3-1 hly-1) was transformed into E. coli, it caused hemolysis on erythrocyte agar plates, but in liquid assays no extemal or intemal hemolytic activity could be detected with the E. coli transformants. B. subtilis carrying the same plasmid exhibited hemolytic activity at Ievels comparable to those ofthe B. cereus donor strain. The hemolysin produced in B. subtilis seemed to be indistinguishable from cereolysin in its sensitivity to cholesterol, activation by dithiothreitol, and inactivation by antibodies raised ...
Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. coli) can only contain one fosmid molecule. Fosmids can hold DNA inserts of up to 40 kb in size; often the source of the insert is random genomic DNA. A fosmid library is prepared by extracting the genomic DNA from the target organism and cloning it into the fosmid vector. The ligation mix is then packaged into phage particles and the DNA is transfected into the bacterial host. Bacterial clones propagate the fosmid library. The low copy number offers higher stability than vectors with relatively higher copy numbers, including cosmids. Fosmids may be useful for constructing stable libraries from complex genomes. Fosmids have high structural stability and have been found to maintain human DNA effectively even after 100 generations of bacterial growth. Fosmid clones were used to help assess the accuracy of the Public Human Genome Sequence. The fertility plasmid or F-plasmid ...
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Examples are cosmids, phagemids, and phasmids. Transposons: They are DNA sequences that can move and replicate in different ...
... ; occipitotemporal sulcus (OTS); collateral sulcus (CoS); mid-fusiform sulcus (MFS). The fusiform gyrus can be ...
Once inside the host, the cosmids circularize with the aid of the host's DNA ligase and then function as plasmids. Cosmids are ... This sequence allows the cosmid to be packaged into bacteriophage λ particles. These particles- containing a linearized cosmid ... Cosmid vectors are plasmids that contain a small region of bacteriophage λ DNA called the cos sequence. ...
Alu repeats and polymorphisms in cosmids sequenced from chromosome 4p16.3". Nature Genetics. 1 (5): 348-53. doi:10.1038/ng0892- ...
Kim UJ, Shizuya H, de Jong PJ, Birren B, Simon MI (March 1992). "Stable propagation of cosmid sized human DNA inserts in an F ... Fosmids are similar to cosmids but are based on the bacterial F-plasmid. The cloning vector is limited, as a host (usually E. ... The major advantage of Fosmids over other cosmid systems lies in its capability of stably propagating human DNA fragments. ... The low copy number offers higher stability than vectors with relatively higher copy numbers, including cosmids. Fosmids may be ...
The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes. Of these, the most commonly ... cosmid, Lambda phages). A vector containing foreign DNA is termed recombinant DNA. ...
... indicating it is on the cosmid 'F38H4', and there are at least 6 other genes on that cosmid. If a gene produces a protein that ... For example, in 2005 a 39 kb cosmid had to be inverted. Other improvements have come from comparing genomic DNA to cDNA ... These species therefore do not have sequence names for CDSs and gene transcripts that are based on cosmid names. Instead they ... which is derived from the cosmid, fosmid or YAC clone on which they reside, for instance F38H4.7, ...
"Physical mapping of the mitochondrial genome of Arabidopsis thaliana by cosmid and YAC clones". Plant Journal. 6 (3): 447-455. ...
1982). "Isolation of human oncogene sequences (v-fes homolog) from a cosmid library". Science. 216 (4550): 1136-8. doi:10.1126/ ...
It is encoded by the P87050 2247 bp ORF on the cosmid 57A10. The protein is 775 amino acids in length. Cdr2 is a member of the ...
A cosmid library (31 249 bp in total) has been made from the genome. The estimated gene density based on the cosmid library is ...
1990). "High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones". Science. 247 (4938): 64-9 ...
June 1994). "Isolation of cosmid and cDNA clones in the region surrounding the BTK gene at Xq21.3-q22". Genomics. 21 (3): 517- ...
... a 1 Mbp cosmid and PAC contig". Genes, Chromosomes & Cancer. 31 (3): 228-39. doi:10.1002/gcc.1139. PMID 11391793. S2CID ...
1999). "A 500-kb sequence-ready cosmid contig and transcript map of the MEN1 region on 11q13". Genomics. 55 (1): 49-56. doi: ...
Cosmid/BAC/YAC end sequences use Cosmid/Bacterial artificial chromosome/Yeast artificial chromosome to sequence the genome from ... Cosmid/BAC/YAC can also be used to get bigger clone of DNA fragment than vectors like plasmid and phagemid. A larger insert is ... BAC can't do that, but BACs can reliably represent human DNA much better than YAC or cosmid. Exon trapped sequence is used to ... DDBJ-GSS MEGA- and GIGA preps of cosmid-, BAC-, PAC, YAC-, and P1-DNA with JETSTAR 2.0 "WSSP-04 Chapter 2 - Vectors" (PDF). ...
1994). "Isolation of cosmid and cDNA clones in the region surrounding the BTK gene at Xq21.3-q22". Genomics. 21 (3): 517-24. ...
The sequence was obtained by a combined approach, employing automated DNA sequence analysis of selected cosmids and whole- ...
... cytogenetic band location of 540 cosmids and 70 genes or DNA markers". Genomics. 15 (1): 133-45. doi:10.1006/geno.1993.1021. ...
January 1993). "Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and ...
Mar 1993). "Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 ...
To clone longer lengths of DNA, lambda phage with lysogeny genes deleted, cosmids, bacterial artificial chromosomes, or yeast ...
1996). "A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome ...
A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol ...
... five cosmids should be used for microdeletion detection and low number of protein truncating mutations". Journal of Medical ...
Cosmid End-sequence profiling Fosmid Human artificial chromosome Yeast artificial chromosome O'Connor M, Peifer M, Bender W ( ...
For instance, phage TM4 was used to construct shuttle phasmids that replicate as large cosmids in Escherichia coli and as ...
Phil Green for the assembly of cosmids in large-scale cosmid shotgun sequencing within the Human Genome Project. Phrap has been ... Typically, Phrap had no problems differentiating between the different Alu copies in a cosmid, and to correctly assemble the ... cosmids (or, later, BACs). The logic is simple: a base call with a high probability of being correct should never be aligned ...
1993). "Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 ...
The Human Genome Project found this application vital to mapping the cosmid clones they were sequencing, and to coordinating ... or cosmid (depending on size) or the genetic material of another organism. Bacterial colonies (such as E. coli) can be rapidly ...
... cells and we can make good cosmid DNA from them. However, after 3-4 weeks , the yield of cosmid DNA from cells derived from the ... It is NO NEED to pick up a single colony for your cosmid preparation each time. After you have checked and know that the cosmid ... Has anyone else had this problem? Are we leaving something out of the , media? Are the cosmid-containing cells less viable? , , ... working with cosmids. Barry Bowman bowman at biology.ucsc.edu Tue Jun 20 19:26:42 EST 1995 *Previous message: only test ...
Depending on the particular aim of the experiment, broad host range cosmids, shuttle cosmids or mammalian cosmids (linked to ... Cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. Cosmids can contain 37 ... Selection against wild type cosmid DNA is simply done via size exclusion. Cosmids, therefore, always form colonies and not ... is held by the terminase after the previous cosmid has been packaged, to prevent degradation by cellular DNases. Cosmids are ...
... Dave Fruhling Fruhlid at war.wyeth.com Tue May 9 15:08:03 EST 2000 *Previous message: Sequencing from ...
Medical definition of cosmid: a plasmid into which a short nucleotide sequence of a bacteriophage has been inserted to create a ... Resources for cosmid. Time Traveler: Explore other words from the year cosmid first appeared Time Traveler! Explore the year a ... Comments on cosmid. What made you want to look up cosmid? Please tell us where you read or heard it (including the quote, if ... Post the Definition of cosmid to Facebook Share the Definition of cosmid on Twitter ...
If I want to pick a good cosmid to inject for mutant rescue, how can I tell with acedb which cosmid will have the ORF lying ... I am interested in a predicted gene that seems to be at one end of a sequenced cosmid, perhaps even extending from one cosmid ... Interpreting cosmid overlaps with acedb. Curtis Loer cloer at acusd.edu Mon Aug 25 16:17:22 EST 1997 *Previous message: Cell ... could someone help me with interpreting the fingerprinting info to pick a few good cosmids? Thanks. Curtis M. Loer Dept. of ...
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Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA.. Rao RN, Richardson MA, Kuhstoss S. ...
... "sequence overlap with cosmid g8261" misc_feature 7505..7506 /note="site of apparent internal deletion in cosmid" BASE COUNT ... REFERENCE 1 (bases 1 to 11862) AUTHORS Goodman,H.M. and Gallant,P. TITLE Sequence of cosmid g17311 from Arabidopsis thaliana ... LOCUS ATU53502 11862 bp DNA PLN 05-MAY-1996 DEFINITION Arabidopsis thaliana chromosome I cosmid g17311 DNA. ACCESSION U53502 ...
LOCUS ATU53501 37570 bp DNA PLN 06-MAY-1996 DEFINITION Arabidopsis thaliana chromosome I cosmid g8261 DNA (cytosine-5-) ...
TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of ... High efficiency cosmid library construction is accomplished using easy and highly repr,biological,biology supply,biology ... TNC Deletion Cosmid Transposition Kit from EPICENTRE Biotechnologies,The pWEB:: ... The pWEB::TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid ...
One cosmid in set A contains the oriL locus in a nonoverlapping region and lacks ori+L forms. This set generated only ori-L ... Two cosmids common to sets B and C ostensibly contain one of the origins of viral DNA replication (oriL) in a region of overlap ... A cosmid-based system for constructing mutants of herpes simplex virus type 1.. Cunningham C1, Davison AJ. ... Cosmids containing large fragments of herpes simplex virus type 1 DNA were prepared using a vector that allows intact inserts ...
250 people for cosmid: High School, Busty, Babes, Galleries, TX, CA, Videos, Pics Galleries, Profile, University, free people ... 05.02.2012 Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid Jeannine Hansen Cosmid ... More of Holly Riley and other busty girls next door at Cosmid · Holly Riley Cosmid 1 Holly Riley Cosmid 2 Holly Riley Cosmid 3 ... Christine carollo cosmidhristine carollo cosmid: Christine carollo cosmidhristine carollo cosmid. Relaxinol ingredients. You ...
Cosmids were provided by the European Drosophila Genome Project (EDGP).. EDGP cosmids are available from Geneservice in the UK ... Cosmids are available either singly or on high-density filters.. The members of the European Drosophila Genome mapping ... Contact for cosmids:. Dr. I. Siden-Kiamos, Institute of Molecular Biology and Biotechnology, FORTH, Heraklion, Crete. Telephone ...
When plasmids or cosmids are larger than 10 kb, pre-heat Buffer EB (or water) to 70°C prior to eluting DNA from the QIAprep ... All QIAprep Miniprep Kits can be used for preparation of low-copy number plasmids and cosmids up to 50 kb. Below are ... Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? ...
One colony: One library cosmid DNA miniprep. Because of the instability of the Olson cosmids, we have divised a miniprep ... Both the cosmid DNA and the E. coli DNAare denatured. However, only the cosmid DNA is supercoiled. Its two strands cannot ... The amount of cosmid DNA prepared is modest, but is more than enough to make an M13 library. While most genomists focus on the ... If you dont select the right E. coli colony, you cannot prepare the right cosmid DNA. 1-Late in your working day streak ...
Misc.Comments : Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is: gtmR - EcoRI/MCS/HindIII - P15A - cos - traJ - oriT - sacB. [1] Deposited by: M.F.Hynes Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C ...
The pWEB™ Cosmid Cloning Kit facilitates rapid and efficient construction of cosmid libraries using the pWEB Cosmid Vector (Fig ... pWEB™ Cosmid Cloning Kit. Notice: The pWEB Cosmid Cloning Kit has been discontinued.. As alternative, please consider the ... Figure 2 (click to enlarge). Outline of the cosmid cloning procedure using the pWEB-TNC™ and the pWEB™ Cosmid Cloning Kits.. ... The result is a complete and unbiased primary cosmid library.. Epicentre also offers kits for fosmid cloning and library ...
The cosmid vectors either contain a Hph marker (pCIT03), or a kanamycin-resistance marker (pCIT101-104). Three of the cosmid ... The others are all cosmid vectors which allow insertion of up to 46 kb of plant genomic DNA, and which also contain all of the ... The other two cosmid vectors (pCIT102 and pCIT104) carry restriction sites flanking the insertion site (XhoI) for convenient ... Hong Ma, Martin F. Yanofsky, Harry J. Klee, John L. Bowman, Elliot M. Meyerowitz, Vectors for plant transformation and cosmid ...
how to get cosmid DNA back to E.coli? - (Aug/07/2006 ). Hi,. I need to know if it is possible to transform E.coli with cosmid ... I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)?. If anyone has a protocol or a ... I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)?. If anyone has a protocol or a ... I have used this method for transforming 21 kb cosmid. I hope this helps.. uhsna. quote name=puma date=Aug 7 2006, 12:27 PM ...
Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments.. B Tomkinson, E Robertson, R Yalamanchili, R Longnecker, E ... Epstein-Barr virus recombinants from overlapping cosmid fragments. Message Subject (Your Name) has forwarded a page to you from ...
... a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, ... High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe Cell. 1993 Apr 9;73(1):109-20. doi: ... Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both ... and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping ...
cosmid definition: Genetics A hybrid vector that has been spliced with plasmid DNA for cloning huge genes or gene fragments.; a ... Definition for "cosmid"*Genetics A hybrid vector that has been spliced… ... How would you define cosmid?. All the definitions on AZdictionary were written by people just like you. Nows your chance to ... Sentence for "cosmid"*E. coli and 25 µg/ml for… ... Medical Dictionary for "cosmid"*DNA from a bacterial ...
Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small ...
... nucleotide sequence of the 16,009-bp SacBII Kan domain of the P1 pAD10-SacBII cloning vector and the sequences of three cosmid ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ... The Complete Nucleotide Sequences of the pSacBII P1 Cloning Vector and Three Cosmid Cloning Vectors: pTCF, svPHEP, and ...
"Isolation of coding sequences from bovine cosmids by means of exon trapping, Mammalian Genome" on DeepDyve, the largest online ... Isolation of coding sequences from bovine cosmids by means of exon trapping. Isolation of coding sequences from bovine cosmids ... Isolation of coding sequences from bovine cosmids by means of exon trapping. Comincini, S.; Drisaldi, B.; Ferretti, L. ... Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for ...
... a cosmid library was constructed and then ordered by a combination of fingerprinting and hybridization techniques. The genome ... Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. Eiglmeier, K ; Honoré, N ; Woods, ... In an attempt to unify the genetic and biological research on Mycobacterium leprae, the aetiological agent of leprosy, a cosmid ... Home , Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. ...
The isolation and sample sequencing of a set of 11 cosmid clones that cover all of one and much of a second large PAI in the ... Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing.. D L Swenson, N O ... To identify possible genes unique to these PAIs, 100 independent subclones of the cosmids were made by PstI digestion and ... Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. ...
Cosmid derived map of escherichia coli strain bhb2600 in comparison to the map of strain w3110 ... Cosmid-derived map of E.coli strain Bhb2600 in comparison to the map of strain W3110. Nucleic Acids Research 17(13): 5057-5070 ... Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110. Nucleic Acids Research 17(13): 5057-5069 ... Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome. Journal of Bacteriology 171(2): ...
HOME TO THE FAMOUS COSMID GIRLS, REAL GIRLS, REAL PHOTOGRAPHERS, REAL LIFE - UPDATED AT LEAST ONCE EVERY STINKING DAY ...
Antonyms for cosmid. 3 words related to cosmid: vector, genetic science, genetics. What are synonyms for cosmid? ... Words related to cosmid. (genetics) a large vector that is made from a bacteriophage and used to clone genes or gene fragments ... The genes are found on a single region of the LB400 chromosome and were originally cloned into a broad host-range cosmid ( ... The positions of the CMT1A-REP sequences were confirmed on the BAC clones using sequences of the cosmid clone c74F4 for the ...
  • These extracts will recognize and package the recombinant molecules in vitro, generating either mature phage particles (lambda-based vectors) or recombinant plasmids contained in phage shells (cosmids). (wikipedia.org)
  • Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA. (nih.gov)
  • The others are all cosmid vectors which allow insertion of up to 46 kb of plant genomic DNA, and which also contain all of the necessary sequences for A. tumefaciens-mediated plant transformation. (caltech.edu)
  • The cosmid vectors either contain a Hph marker (pCIT03), or a kanamycin-resistance marker (pCIT101-104). (caltech.edu)
  • Three of the cosmid vectors (pCIT30, pCIT101, and pCIT103) carry bacteriophage T7 and SP6 promoters flanking the cloning Bg/II site, for synthesis of end-specific RNAs. (caltech.edu)
  • The other two cosmid vectors (pCIT102 and pCIT104) carry restriction sites flanking the insertion site (XhoI) for convenient release of the insert by restriction digests. (caltech.edu)
  • The complete nucleotide sequence of the 16,009-bp SacBII Kan domain of the P1 pAD10-SacBII cloning vector and the sequences of three cosmid cloning vectors, pTCF (7941 bp), svPHEP (9201 bp), and LAWRIST16 (5194 bp) have been determined. (rti.org)
  • Cosmids were designed as vectors able to incorporate DNA fragments up to 40-50 kb in size. (enacademic.com)
  • Cosmids are ordinarily plasmid vectors with the intention of be inflicted with cos sites. (ebioworld.com)
  • How can you duplicate in to cosmid vectors? (ebioworld.com)
  • Because bacteriophage contaminants may possibly take amongst 38 and 53 kilobytes regarding Geonomics and as generally cosmids are regarding 5 kilobytes, among 33 and 48 kb of Geonomics may possibly cloned in these vectors. (ebioworld.com)
  • 2000). Cosmid vectors have also been used for rapid genomic walking, gene transfer, restriction mapping and determining the functional and structural organization of complex eukaryotic genomes (Wahl et al. (bartleby.com)
  • They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. (springer.com)
  • cosmids or other cloning vectors. (bio-medicine.org)
  • Cosmid vectors can package about 44 kb. (encyclopedia.com)
  • Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. (atcc.org)
  • The loading capacity of cosmids varies depending on the size of the vector itself but usually lies around 40-45 kb. (wikipedia.org)
  • TNC Cosmid Vector, packaged using ultra-high efficiency MaxPlax Lambda Packaging Extracts (>10 9 pfu/ m g for phage lambda), also in the kit, and plated on the supplied EPBOS plating cells. (bio-medicine.org)
  • Cosmids containing large fragments of herpes simplex virus type 1 DNA were prepared using a vector that allows intact inserts to be excised using the restriction endonuclease PacI. (nih.gov)
  • The pWEB™ Cosmid Cloning Kit facilitates rapid and efficient construction of cosmid libraries using the pWEB Cosmid Vector (Fig. 1). (epibio.com)
  • Hi, I need to know if it is possible to transform E.coli with cosmid vector DNA (approx 30 kb)? (protocol-online.org)
  • To identify possible genes unique to these PAIs, 100 independent subclones of the cosmids were made by PstI digestion and ligation into a pBS+ plasmid and used in one-pass sample DNA sequencing from primer binding sites at the cloning site in the vector DNA. (asm.org)
  • The complete nucleotide sequence of pO113 from EH41 was determined from a series of overlapping 30-40 kb fragments cloned into the Copy Control pCC1FOS cosmid vector (Epicentre, Madison, WI, USA) and propagated in E. (freethesaurus.com)
  • Cosmid - A cosmid, first described by Collins and Hohn in 1978, is a type of hybrid plasmid (often used as a cloning vector) that contains cos sequences, DNA sequences originally from the Lambda phage. (enacademic.com)
  • cosmid - A type of bacteriophage lambda vector. (enacademic.com)
  • The construction of a double-cos-site cosmid vector, pMOcosX, for use in making filamentous fungal genomic DNA libraries, is described. (elsevier.com)
  • Construction and functional screening of a metagenomic library using a T7 RNA polymerase-based expression cosmid vector. (archives-ouvertes.fr)
  • In this study, the shuttle cosmid vector pFX583 was used for the construction and screening of a metagenomic library. (archives-ouvertes.fr)
  • We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin / streptomycin / chloramphenicol -resistant cosmid vector. (bvsalud.org)
  • Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, were ordered by hybridizing genetic markers and individual clones from the two libraries. (nih.gov)
  • Yeast artificial chromosome (YAC) clones covering the entire genome were used to subdivide the libraries, and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping information. (nih.gov)
  • Construction of soil environmental DNA cosmid libraries and screening for clones that produce biologically active small molecules. (biomedsearch.com)
  • A modified diatomaceous earth (Prep-A-Gene)-based procedure, which rapidly yields highly supercoiled double-stranded DNA from recombinant P1 and cosmid clones suitable for generating shotgun libraries, also has been developed. (rti.org)
  • The isolation and sample sequencing of a set of 11 cosmid clones that cover all of one and much of a second large PAI in the uropathogenic E. coli J96 are described. (asm.org)
  • erythraea was derived from 256 canonically ordered cosmid clones representing a circular genome of 8. (freethesaurus.com)
  • Direct carrier detection by in situ suppression hybridization with cosmid clones of the Duchenne/Becker muscular dystrophy locus. (freethesaurus.com)
  • The positions of the CMT1A-REP sequences were confirmed on the BAC clones using sequences of the cosmid clone c74F4 for the proximal CMT1A-REP (17) and c15H12 for the distal CMT1A-REP sequence (31). (freethesaurus.com)
  • Restoration of virulence was achieved by complementation with cosmid clones spanning a 30-kb region of the plasmid that contained previously identified avirulence ( avr ) genes avrD , avrPphC , and avrPphF . (pnas.org)
  • Five cosmid clones with highest simultaneous interfering activities were further analyzed and the respective open reading frames conferring QQ activities identified. (frontiersin.org)
  • The high-density map of chromosome 1p constructed here can provide useful landmarks for constructing a contig map of the short arm of chromosome 1 with YACs and cosmid clones and will expedite the identification of breakpoints and/or tumor suppressor gene(s) associated with several types of malignant tumors that frequently exhibit chromosomal aberrations or deletions of chromosome 1p. (elsevier.com)
  • A few weeks ago I described some problems we were having with cosmid libraries. (bio.net)
  • Cosmids can be used to build genomic libraries. (wikipedia.org)
  • After the construction of recombinant lambda or cosmid libraries the total DNA is transferred into an appropriate E. coli host via a technique called in vitro packaging. (wikipedia.org)
  • Phage libraries are also stored and screened more easily than cosmid libraries. (wikipedia.org)
  • Preparation of complete, unbiased cosmid libraries. (epibio.com)
  • Significantly, our results also indicate the use of cosmid libraries for the genetic manipulation of bacteria can lead to phenotypes not necessarily linked to the gene(s) of interest. (northumbria.ac.uk)
  • Cosmid libraries are used for cloning genes with large introns and for sequencing larger chunks of the genome. (bartleby.com)
  • Constructions of gene libraries of Rana nigromaculata and Mus musculus with cosmid pHC79. (cngb.org)
  • These strains were used to amplify packaged recombinant cosmid libraries of Mycobacterium leprae, Mycobacterium vaccae, Salmonella typhimurium, and Streptococcus mutans DNA. (elsevier.com)
  • Construction of noncontiguous libraries allowed the dissociation of desired genes from genes that were deleterious to the survival of a cosmid recombinant and permitted selection for unliked traits that resulted in a selected phenotype. (elsevier.com)
  • In vivo repackaging of recombinant cosmids permitted (i) amplification of the original in vitro-packaged collection of transducing particles, (ii) storage of cosmid libraries as phage lysates, (iii) facilitation of complementation screening, (iv) expression analysis of repackaged recombinant cosmids after UV-irradiated cells were infected, (v) in situ enzyme or immunological screening, and (vi) facilitation of recovery of recombinant cosmid molecules containing transposon inserts. (elsevier.com)
  • pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries . (bvsalud.org)
  • Cosmids are predominantly plasmids with a bacterial oriV, an antibiotic selection marker and a cloning site, but they carry one, or more recently two, cos sites derived from bacteriophage lambda. (wikipedia.org)
  • Can I use QIAprep Miniprep kits for low-copy plasmids and cosmids? (qiagen.com)
  • All QIAprep Miniprep Kits can be used for preparation of low-copy number plasmids and cosmids up to 50 kb. (qiagen.com)
  • Thus cosmids are essentially plasmids which have had a small piece of bacteriophage lambda DNA including the cos site (that is, containing the single stranded cohesive/sticky endings of λ phage which are complimentary to each other) cloned into them (Lodge, Lund & Minchin, 2007). (bartleby.com)
  • The development at Oncor of a Her-2/neu cosmid clone suitable for fluorescence in situ hybridization is an important new development. (freethesaurus.com)
  • Kempski, H & Cowell, JK 1993, ' Detection of submicroscopic chromosomal deletions in aniridia patients using fluorescence in situ hybridization and a panel of cosmids covering the WT1 gene ', International journal of oncology , vol. 3, no. 5, pp. 937-940. (elsevier.com)
  • To produce a reliable probe suitable for aneuploidy detection of chromosome13 on uncultured lymphocytes and amniocytes by fluorescence in situ hybridization [FISH], we used a contig of three overlapping cosmids mapped to 13q12.3. (bvsalud.org)
  • Fifty-five individual cosmids isolated from a chromosome 13-specific library have been regionally assigned using fluorescence in situ hybridization. (elsevier.com)
  • A high-resolution cytogenetic map of the short arm of chromosome 1 with newly isolated 411 cosmid markers was constructed by fluorescence in situ hybridization (FISH). (elsevier.com)
  • The goals of this project are: (1) to create a cosmid genomic library of the red swamp crayfish, Procambarus clarkii, (2) to clone and characterize genes involved in skeletal muscle growth of the crayfish, and (3) to identify genetic markers in the crayfish genome that can potentially be used in the selection of individuals with economically desirable traits. (freethesaurus.com)
  • Construction of a 350-kb sequence-ready 11q13 cosmid contig encompassing the markers D11S4933 and D11S546: mapping of 11 genes and 3 tumor-associated translocation breakpoints. (freethesaurus.com)
  • The majority of the interval has also been converted to a cosmid contig. (elsevier.com)
  • These cosmids represent novel DNA markers that can be added to the limited number of DNA probes already available for this chromosome. (elsevier.com)
  • Individual cosmids mapped along the length of the chromosome with almost equal distribution except that there appears to be an excess of probes from the distal 13q33-q34 region. (elsevier.com)
  • Ferretti, L. 2009-03-24 00:00:00 Exon trapping was employed to identify coding sequences from a collection of 46 bovine cosmids, previously characterized for the presence of microsatellite markers and physically mapped to chromosomes by FISH. (deepdyve.com)
  • The cosmid DNA carrying the expected sequences of human chromosome 13 was isolated from host cells and labelled with biotin -11-dUTP. (bvsalud.org)
  • Two pathogenicity islands in uropathogenic Escherichia coli J96: cosmid cloning and sample sequencing. (asm.org)
  • Created with DNA from a bacteriophage (a virus that infectsbacteria) combined with genetic material called a cosmid from the common bacterium Escherichia coli, the shuttle phasmid can infect mycobacteria and grow in E. (freethesaurus.com)
  • Strains of Escherichia coli K-12 were constructed that permitted the amplification of in vitro-packaged recombinant cosmid-transducing particles by in vivo repackaging of recombinant cosmid molecules. (elsevier.com)
  • I am interested in a predicted gene that seems to be at one end of a sequenced cosmid, perhaps even extending from one cosmid to the next adjacent. (bio.net)
  • Overall, the type 1 EBNA 3A gene was incorporated into 26% of the transformation marker-rescued recombinants, a frequency which was considerably higher than that observed in previous experiments with two-cosmid EBV DNA cotransfections into P3HR-1 cells (B. Tomkinson and E. Kieff, J. Virol. (asm.org)
  • Of the recombinants which had incorporated the marker-rescuing cosmid DNA fragment and the fragment encoding the type 1 EBNA 3A gene, most had incorporated markers from at least two other transfected cosmid DNA fragments, indicating a propensity for multiple homologous recombinations. (asm.org)
  • The frequency of incorporation of the nonselected transfected type 1 EBNA 3C gene, which is near the end of two of the transfected cosmids, was 26% overall, versus 3% in previous experiments using transfections with two EBV DNA cosmids. (asm.org)
  • Orbach, MJ 1994, ' A cosmid with a Hy R marker for fungal library construction and screening ', Gene , vol. 150, no. 1, pp. 159-162. (elsevier.com)
  • A series of cosmids have been isolated from a human chromosome 11-specific cosmid library using the human Wilms tumour predisposition gene cDNA, WT33. (elsevier.com)
  • The one recombinant virus examined in detail by Southern blot analysis has all the polymorphisms characteristic of the transfected type 1 cosmid DNA and none characteristic of the type 2 P3HR-1 EBV DNA. (asm.org)
  • Thermal induction of these thermoinducible, excision-defective lysogens containing recombinant cosmid molecules yielded high titers of packaged recombinant cosmids and low levels of PFU. (elsevier.com)
  • These results indicate that a cosmid-based system will facilitate isolation of large numbers of defined viral mutants. (nih.gov)
  • cells and we can make good cosmid DNA from them. (bio.net)
  • If I want to pick a good cosmid to inject for mutant rescue, how can I tell with acedb which cosmid will have the ORF lying more-or-less in the center instead of at the extreme of one end? (bio.net)
  • Because of the instability of the Olson cosmids, we have divised a miniprep procedure that minimizes E. coli growth, thus allowing the least possible time for cosmid DNA deletions/rearrangements to arise and be selected. (stanford.edu)
  • If you don't select the 'right' E. coli colony, you cannot prepare the 'right' cosmid DNA. (stanford.edu)
  • If you see all large colonies, proceed with caution, as you may have E. coli containing a cosmid with deletions. (stanford.edu)
  • First thing in the morning, harvest the E. coli and prepare cosmid DNA. (stanford.edu)
  • Both the cosmid DNA and the E. coli DNAare denatured. (stanford.edu)
  • When the solution is brought to neutral pH, the cosmid DNA renatures spontaneously, while the E. coli DNA does not renature under these conditions. (stanford.edu)
  • how to get cosmid DNA back to E.coli? (protocol-online.org)
  • A physical map for the genome of E. coli K12 strain BHB2600 was constructed by use of 570 cloned DNA elements (CDEs) withdrawn from a cosmid library. (eurekamag.com)
  • A cosmid is a type of hybrid plasmid that contains a Lambda phage cos sequence. (wikipedia.org)
  • You could also take advantage of the fact that it's a cosmid, and use a lambda packaging kit. (protocol-online.org)
  • 1. Given with the intention of repeats happens to eukaryotic Geonomics rearrangements can take place via recombination from the repeats current in this area the Geonomics inserted in to lambda or cosmid. (ebioworld.com)
  • Epstein-Barr virus recombinants from overlapping cosmid fragments. (asm.org)
  • Five overlapping type 1 Epstein-Barr virus (EBV) DNA fragments constituting a complete replication- and transformation-competent genome were cloned into cosmids and transfected together into P3HR-1 cells, along with a plasmid encoding the Z immediate-early activator of EBV replication. (asm.org)
  • These recombinants probably result from recombination among the transfected cosmid-cloned EBV DNA fragments. (asm.org)
  • These cosmids, therefore, can be used to analyse chromosomes from patients with sporadic aniridia for submicroscopic deletions. (elsevier.com)
  • TNC Deletion Cosmid Cloning Kit enables you to prepare, in about two days, a complete and unbiased primary cosmid library of genomic DNA for subsequent use in making nested deletion sublibraries. (bio-medicine.org)
  • Notice: The pWEB Cosmid Cloning Kit has been discontinued. (epibio.com)
  • In an attempt to unify the genetic and biological research on Mycobacterium leprae, the aetiological agent of leprosy, a cosmid library was constructed and then ordered by a combination of fingerprinting and hybridization techniques. (epfl.ch)
  • Cosmids can contain 37 to 52 (normally 45) kb of DNA, limits based on the normal bacteriophage packaging size. (wikipedia.org)
  • Outline of the cosmid cloning procedure using the pWEB-TNC™ and the pWEB™ Cosmid Cloning Kits. (epibio.com)
  • 7505 /note="sequence overlap with cosmid g8261" misc_feature 7505. (harvard.edu)
  • LOCUS ATU53501 37570 bp DNA PLN 06-MAY-1996 DEFINITION Arabidopsis thaliana chromosome I cosmid g8261 DNA (cytosine-5-) methyltransferase, zinc finger protein 1, nucleoporin 98, poly A+ RNA export protein, plasma membrane ATPase 2, and serine/threonine protein kinase genes, complete cds. (harvard.edu)
  • Depending on the particular aim of the experiment, broad host range cosmids, shuttle cosmids or 'mammalian' cosmids (linked to SV40 oriV and mammalian selection markers) are available. (wikipedia.org)
  • While most genomists focus on the various published biochemical procedures for preparing cosmid DNA, they overlook microbiology, and the microbiology is the most important part of preparing cosmid DNA. (stanford.edu)
  • IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. (atcc.org)
  • We have been working with the Sachs/Orbach cosmid library for Neurospora. (bio.net)
  • High efficiency cosmid library construction is accomplished using easy and highly reproducible kit protocols. (bio-medicine.org)
  • The amount of cosmid DNA prepared is modest, but is more than enough to make an M13 library. (stanford.edu)
  • The result is a complete and unbiased primary cosmid library. (epibio.com)
  • Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. (epfl.ch)
  • A strategy for high-volume sequencing of cosmid DNAs: random and direct priming with a library of oligonucleotides. (freethesaurus.com)
  • Only two cosmids did not map back to chromosome 13, indicating that at least 96% of cosmids in this library are from this chromosome. (elsevier.com)
  • Cosmids were provided by the European Drosophila Genome Project (EDGP). (fruitfly.org)
  • Cosmid - DNA from a bacterial virus into which is spliced a small fragment of a genome to be amplified and sequenced. (enacademic.com)
  • These cosmids shared a 7-kb DNA fragment containing an open reading frame encoding a protein with similarity to the nucleotide binding site leucine-rich repeat family of resistance genes. (plantcell.org)
  • In Y. pestis , a 102-kb chromosomal fragment ( pgm locus) that carries genes involved in iron acquisition and colony pigmentation can be deleted en bloc. (asm.org)
  • LOCUS ATU53502 11862 bp DNA PLN 05-MAY-1996 DEFINITION Arabidopsis thaliana chromosome I cosmid g17311 DNA. (harvard.edu)
  • One cosmid in set A contains the oriL locus in a nonoverlapping region and lacks ori+L forms. (nih.gov)
  • Genetic complementation analysis in transgenic R 1 plants using a set of overlapping cosmids covering the I2 locus revealed three cosmids giving full resistance to F. o. lycopersici race 2. (plantcell.org)
  • This causes linearization of the circular cosmid with two "cohesive" or "sticky ends" of 12bp. (wikipedia.org)
  • Cosmid - Als Cosmide bezeichnet man Plasmide, die sogenannte cos sites (cohesive site) enthalten. (enacademic.com)
  • Seven overlapping cosmids were isolated which cover the genomic sequence of WT1 and in situ hybridisation shows that they all localise to the p13 region of chromosome 11. (elsevier.com)
  • Viral mutants with lesions in either or both of genes UL2 and UL44, which are not essential for growth in cell culture, were constructed using cosmids containing specifically introduced frameshift mutations. (nih.gov)
  • EBV recombinants which have the type 1 EBNA LP and 2 genes from the transfected EcoRI-A cosmid DNA were selectively and clonally recovered by exploiting the unique ability of the recombinants to transform primary B lymphocytes into lymphoblastoid cell lines. (asm.org)
  • The genes are found on a single region of the LB400 chromosome and were originally cloned into a broad host-range cosmid (pGEM410). (freethesaurus.com)
  • We provide evidence that they are induced by introducing the cosmid St4A10∆lsp, as part of ReDirect PCR mutagenesis protocol, which transiently duplicates a number of important cell division genes. (northumbria.ac.uk)
  • The cosQ site of next cosmid (as rolling circle replication often results in linear concatemers) is held by the terminase after the previous cosmid has been packaged, to prevent degradation by cellular DNases. (wikipedia.org)
  • Intensive signals were detected when a combination of three overlapping cosmids was used to enumerate the chromosome 13 on interphase nuclei. (bvsalud.org)
  • After 4-6 weeks the clone has lost the amp-containing cosmid and becomes ampicillin sensitive. (bio.net)
  • Later cosmids do have clone left and right ends as this became part of the standard procedure. (wormbase.org)
  • Two independent sets (A and B) of five cosmids were identified whose inserts overlap and represent the entire viral genome, and set C was obtained by replacing two cosmids in set B. Each set gave rise to viral plaques when digested with PacI and transfected into cells in culture. (nih.gov)
  • Two cosmids common to sets B and C ostensibly contain one of the origins of viral DNA replication (oriL) in a region of overlap between inserts, but both actually consist of a minority of apparently intact (ori+L) forms and a majority of deleted (ori-L) forms. (nih.gov)
  • P3HR-1 cells harbor a type 2 EBV which is unable to transform primary B lymphocytes because of a deletion of DNA encoding EBNA LP and EBNA 2, but the P3HR-1 EBV can provide replication functions in trans and can recombine with the transfected cosmids. (asm.org)
  • The same goes for the lower yield of cosmid DNA after 3-4 weeks, since the colony contains a population of resistant and sensitive cells. (bio.net)
  • It is NO NEED to pick up a single colony for your cosmid preparation each time. (bio.net)