Corynebacterium pseudotuberculosis: A species of gram-positive, asporogenous bacteria that was originally isolated from necrotic areas in the kidney of a sheep. It may cause ulcerative lymphangitis, abscesses, and other chronic purulent infections in sheep, horses, and other warm-blooded animals. Human disease may form from contact with infected animals.Corynebacterium Infections: Infections with bacteria of the genus CORYNEBACTERIUM.Corynebacterium: A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.Yersinia pseudotuberculosis: A human and animal pathogen causing mesenteric lymphadenitis, diarrhea, and bacteremia.Lymphadenitis: Inflammation of the lymph nodes.Yersinia pseudotuberculosis Infections: Infections with bacteria of the species YERSINIA PSEUDOTUBERCULOSIS.Sheep Diseases: Diseases of domestic and mountain sheep of the genus Ovis.Corynebacterium glutamicum: A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.Goat Diseases: Diseases of the domestic or wild goat of the genus Capra.Goats: Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.Corynebacterium diphtheriae: A species of gram-positive, asporogenous bacteria in which three cultural types are recognized. These types (gravis, intermedius, and mitis) were originally given in accordance with the clinical severity of the cases from which the different strains were most frequently isolated. This species is the causative agent of DIPHTHERIA.Phospholipase D: An enzyme found mostly in plant tissue. It hydrolyzes glycerophosphatidates with the formation of a phosphatidic acid and a nitrogenous base such as choline. This enzyme also catalyzes transphosphatidylation reactions. EC 3.1.4.4.Horse Diseases: Diseases of domestic and wild horses of the species Equus caballus.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Sheep: Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.Yersinia: A genus of gram-negative, facultatively anaerobic rod- to coccobacillus-shaped bacteria that occurs in a broad spectrum of habitats.Yersinia pestis: The etiologic agent of PLAGUE in man, rats, ground squirrels, and other rodents.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Horses: Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Yersinia Infections: Infections with bacteria of the genus YERSINIA.Glycolysis: A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.Gluconeogenesis: Biosynthesis of GLUCOSE from nonhexose or non-carbohydrate precursors, such as LACTATE; PYRUVATE; ALANINE; and GLYCEROL.Sulfur: An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.Sulfur Compounds: Inorganic or organic compounds that contain sulfur as an integral part of the molecule.Carbon Cycle: The cycle by which the element carbon is exchanged between organic matter and the earth's physical environment.Chlorobi: A phylum of anoxygenic, phototrophic bacteria including the family Chlorobiaceae. They occur in aquatic sediments, sulfur springs, and hot springs and utilize reduced sulfur compounds instead of oxygen.Chlorobium: A genus of phototrophic, obligately anaerobic bacteria in the family Chlorobiaceae. They are found in hydrogen sulfide-containing mud and water environments.Carbon: A nonmetallic element with atomic symbol C, atomic number 6, and atomic weight [12.0096; 12.0116]. It may occur as several different allotropes including DIAMOND; CHARCOAL; and GRAPHITE; and as SOOT from incompletely burned fuel.Pentose Phosphate Pathway: An oxidative decarboxylation process that converts GLUCOSE-6-PHOSPHATE to D-ribose-5-phosphate via 6-phosphogluconate. The pentose product is used in the biosynthesis of NUCLEIC ACIDS. The generated energy is stored in the form of NADPH. This pathway is prominent in tissues which are active in the synthesis of FATTY ACIDS and STEROIDS.PeptidoglycanMuramic Acids: Compounds consisting of glucosamine and lactate joined by an ether linkage. They occur naturally as N-acetyl derivatives in peptidoglycan, the characteristic polysaccharide composing bacterial cell walls. (From Dorland, 28th ed)Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.DNA Repair: The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.Cockayne Syndrome: A syndrome characterized by multiple system abnormalities including DWARFISM; PHOTOSENSITIVITY DISORDERS; PREMATURE AGING; and HEARING LOSS. It is caused by mutations of a number of autosomal recessive genes encoding proteins that involve transcriptional-coupled DNA REPAIR processes. Cockayne syndrome is classified by the severity and age of onset. Type I (classical; CSA) is early childhood onset in the second year of life; type II (congenital; CSB) is early onset at birth with severe symptoms; type III (xeroderma pigmentosum; XP) is late childhood onset with mild symptoms.Xeroderma Pigmentosum: A rare, pigmentary, and atrophic autosomal recessive disease. It is manifested as an extreme photosensitivity to ULTRAVIOLET RAYS as the result of a deficiency in the enzyme that permits excisional repair of ultraviolet-damaged DNA.Ultraviolet Rays: That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.Xeroderma Pigmentosum Group A Protein: A ZINC FINGER MOTIF protein that recognizes and interacts with damaged DNA. It is a DNA-binding protein that plays an essential role in NUCLEOTIDE EXCISION REPAIR. Mutations in this protein are associated with the most severe form of XERODERMA PIGMENTOSUM.PentosephosphatesPhosphoribosyl Pyrophosphate: The key substance in the biosynthesis of histidine, tryptophan, and purine and pyrimidine nucleotides.Transaldolase: An enzyme of the transferase class that catalyzes the reaction sedoheptulose 7-phosphate and D-glyceraldehyde 3-phosphate to yield D-erythrose 4-phosphate and D-fructose phosphate in the PENTOSE PHOSPHATE PATHWAY. (Dorland, 27th ed) EC 2.2.1.2.LymphangitisProteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Transference (Psychology): The unconscious transfer to others (including psychotherapists) of feelings and attitudes which were originally associated with important figures (parents, siblings, etc.) in one's early life.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).Serial Publications: Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.

Identification of nonlipophilic corynebacteria isolated from dairy cows with mastitis. (1/64)

Nonlipophilic corynebacteria associated with clinical and subclinical mastitis in dairy cows were found to belong to four species: Corynebacterium amycolatum, Corynebacterium ulcerans, Corynebacterium pseudotuberculosis, and Corynebacterium minutissimum. These species may easily be confused. However, clear-cut differences between C. ulcerans and C. pseudotuberculosis were found in their acid production from maltotriose and ethylene glycol, susceptibility to vibriostatic agent O129, and alkaline phosphatase. Absence of growth at 20 degrees C and lack of alpha-glucosidase and 4MU-alpha-D-glycoside hydrolysis activity differentiated C. amycolatum from C. pseudotuberculosis and C. ulcerans. The mastitis C. pseudotuberculosis strains differed from the biovar equi and ovis reference strains and from caprine field strains in their colony morphologies and in their reduced inhibitory activity on staphylococcal beta-hemolysin. C. amycolatum was the most frequently isolated nonlipophilic corynebacterium.  (+info)

Targeting improves the efficacy of a DNA vaccine against Corynebacterium pseudotuberculosis in sheep. (2/64)

A large-scale DNA vaccination trial was performed with sheep to investigate whether an antigen targeted by CTLA-4 enhanced and accelerated the humoral immune response. Vaccination with genetically detoxified phospholipase D (DeltaPLD) has been shown to be effective, at least partially, against Corynebacterium pseudotuberculosis, the causal agent of caseous lymphadenitis in sheep. CTLA-4 binds to B7 on antigen-presenting cells and thus was used to direct the fusion antigens to sites of immune induction. Here we demonstrated that targeting DeltaPLD as a CTLA-4 fusion protein significantly enhanced the speed, magnitude, and longevity of the antibody response compared to that obtained with DNA encoding DeltaPLD. While all groups of sheep vaccinated with DNA encoding DeltaPLD were afforded better protection against an experimental challenge with C. pseudotuberculosis than those immunized with an irrelevant plasmid or those left unimmunized, the best protection was provided by the targeted DNA vaccine. We propose that targeting antigens to antigen-presenting cells offers a generic strategy for enhancing the efficacy of DNA vaccines.  (+info)

Tumor necrosis factor alpha and gamma interferon are required for the development of protective immunity to secondary Corynebacterium pseudotuberculosis infection in mice. (3/64)

The production and role of endogenous cytokines during the course of secondary Corynebacterium (C.) pseudotuberculosis infection were investigated in mice. When immunized mice were challenged on day 28 after primary infection, tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) were found to appear at 3 hr and to reach the maximum at 24 hr after challenge. Spleen cells of mice primarily infected from 2 to 8 weeks before produced a significant amount of TNF-alpha and IFN-gamma when stimulated with formalin-killed bacteria. However, they could not produce detectable amounts of IL-4. The administration of anti-TNF-alpha monoclonal antibody (MAb) and IFN-gamma MAb increased bacterial proliferation in the organs of immune mice and exacerbated the secondary infection. Injection of anti-CD4 MAb alone or anti-CD4 plus anti-CD8 MAbs resulted in significantly increased mortality and a marked suppression of bacterial elimination as well as cytokine production of secondarily infected mice, while the treatment with anti-CD8 MAb alone showed no effect on either the resistance or cytokine production of mice. These results suggest that CD4, probably Th1 T cells, play an important role for establishment of protective immunity against secondary C. pseudotuberculosis infection by secreting TNF-alpha and IFN-gamma.  (+info)

Characterization of United Kingdom isolates of Corynebacterium pseudotuberculosis using pulsed-field gel electrophoresis. (4/64)

Caseous lymphadenitis is a chronic suppurative disease caused by Corynebacterium pseudotuberculosis and is responsible for serious economic losses to the sheep and goat industry. Caseous lymphadenitis was first reported for goats in the United Kingdom in 1990 and for sheep in 1991. Recent evidence suggests that the prevalence of the disease within the national flock is increasing. Fifty isolates of C. pseudotuberculosis from the United Kingdom comprising sheep and horse isolates, the original goat outbreak strain, and the type strain were characterized by biotyping, antimicrobial susceptibility, production of phospholipase D, and genotyping by pulsed-field gel electrophoresis using SfiI and SmaI. All of the isolates were confirmed as C. pseudotuberculosis, and all produced phospholipase D but none reduced nitrate. Restriction with SfiI generated 16 to 18 bands between 48.5 and 290 kb and differentiated six pulsotypes. We conclude that 80% of the strains tested were epidemiologically related to the outbreak strain and that the equine profile was distinct both phenotypically and genotypically.  (+info)

Mechanism of induction of complement susceptibility of erythrocytes by spider and bacterial sphingomyelinases. (5/64)

We have recently shown that the sphingomyelinase toxins P1 and P2 from the venom of the spider Loxosceles intermedia induce complement (C)-dependent lysis of autologous erythrocytes by induction of the cleavage of cell surface glycophorins through activation of an endogenous metalloproteinase facilitating the activation of the alternative pathway of C. Phospholipase D (PLD) from Corynebacterium pseudotuberculosis shows some degree of homology with the spider sphingomyelinases and can induce similar clinical symptoms to those observed after spider envenomation. The aim of this study was to investigate if the bacterial PLD-induced haemolysis of human erythrocytes was C dependent and if cleavage of glycophorins occurred. We show here that haemolysis of both PLD- and P1-treated human erythrocytes was C dependent, but while PLD-mediated haemolysis was dependent on activation of the classical pathway of C, P1 induced lysis via both the classical and alternative pathways. P1, but not PLD, induced cleavage of glycophorins and no change in expression of complement regulators was induced by either of the toxins. In both cases, annexin V binding sites were exposed, suggesting that the membrane asymmetry had been disturbed causing exposure of phosphatidylserine to the cell surface. Our results suggest that C susceptibility induced by L. intermedia and C. pseudotuberculosis PLD is a result of exposure of phosphatidylserine, and the higher potency of P1 toxin can be explained by its additional effect of cleavage of glycophorins.  (+info)

Ovine mononuclear phagocytes in situ: identification by monoclonal antibodies and involvement in experimental pyogranulomas. (6/64)

In order to characterize in situ the macrophages present in experimental pyogranulomas induced in lambs with Corynebacterium pseudotuberculosis, a set of monoclonal antibodies (MAbs) was produced following immunization of BALB/c mice with alveolar macrophages from healthy sheep. Three MAbs were retained after two steps of screening using alveolar macrophages, peripheral blood lymphocytes, and polymorphonuclear leukocytes as target cells. Their reactivity was tested not only on macrophages in pyogranulomas but also on sections of various organs in steady-state conditions. One MAb, termed OM1, recognized the monocytes and the majority of cells of the mononuclear phagocyte system in lymphoid and nonlymphoid organs. The two other MAbs, OM2 and OM3, reacted with a subpopulation of alveolar macrophages and with other cell types in tissues, in particular with endothelial cells for the MAb OM2. On sections of experimental pyogranulomas that developed in lymph nodes draining the C. pseudotuberculosis-injected sites, MAb OM1 reacted with all the macrophages distributed in a palisade surrounding the necrotic center of the lesion from day 6 to day 28 postinoculation. The two other MAbs, OM2 and OM3, enabled two types of granulomas to be distinguished: one type was characterized by a large number of epithelioid cells stained by OM2; and the other was characterized by a few OM2-positive macrophages, whereas the OM3-positive cells were more numerous. These results show that macrophages are predominant cells in pyogranulomas and suggest two different histological patterns in the evolution of pyogranulomas induced by C. pseudotuberculosis, according to the immunological status of the host.  (+info)

Corynebacterium pseudotuberculosis infection in Israeli dairy cattle. (7/64)

Two forms of Corynebacterium pseudotuberculosis infection in Israeli dairy cattle herds during a survey period of 13 years (1989-2001) are described. The more common form, which was diagnosed in 45 herds, was characterized by ulcerative granulomatous lesions which occurred either sporadically--in 26 herds (with a morbidity rate of up to 5%)--or in an epidemic course in 19 herds. Most (80.6%) of the affected animals were cows; the rest were first-calving cows (16.2%) and heifers (3.2%). The morbidity occurred mostly during the summer months. The ulcerative granulomatous lesions appeared in three clinical forms: cutaneous, mastitic and visceral. Mixed forms were also observed. The morbidity rate was 6.4% and the culling rate reached 16.3% of the affected animals. Most of the strains of C. pseudotuberculosis which were isolated from the abscesses in the cutaneous form of the disease and from milk samples failed to reduce nitrate. A decrease in milk production (6%) and an increase in bulk-milk somatic cell count were noted. Necrotic and ulcerative dermatitis on the heel of the foot occurred in an epidemic course in heifers in only two herds during the winter months, with morbidity rates of 7.5 and 76.2%, respectively. C. pseudotuberculosis isolates from skin lesions and from the soil did reduce nitrate. Clinical, epizootiological and microbiological aspects of the infection are described.  (+info)

Rational attenuation of Corynebacterium pseudotuberculosis: potential cheesy gland vaccine and live delivery vehicle. (8/64)

The phospholipase D (PLD) gene (pld) has been deleted from the Corynebacterium pseudotuberculosis chromosome by using site-specific mutagenesis. Sheep infection trials indicate that the PLD-negative C. pseudotuberculosis strain (Toxminus) is incapable of inducing caseous lymphadentis (cheesy gland) even at doses two logs higher than that at which the wild-type strain produces the disease. This clearly establishes PLD as a major C. pseudotuberculosis virulence factor. Vaccination of sheep with live Toxminus C. pseudotuberculosis elicits strong humoral and cell-mediated immune responses and protects the animals from wild-type challenge.  (+info)

Corynebacterium pseudotuberculosis is a pathogenic bacterium which has been rapidly spreading all over the world, causing economic losses in the agricultural sector and sporadically infecting humans. Six C. pseudotuberculosis strains were isolated from goats, sheep, and horses with distinct abscess locations. For the first time, Mexican genomes of this bacterium were sequenced and studied in silico. All strains were sequenced using Ion Personal Genome Machine sequencer, assembled using Newbler and SPAdes software. The automatic genome annotation was done using the software RAST and in-house scripts for transference, followed by manual curation using Artemis software and BLAST against NCBI and UniProt databases. The six genomes are publicly available in NCBI database. The analysis of nucleotide sequence similarity and the generated phylogenetic tree led to the observation that the Mexican strains are more similar between strains from the same host, but the genetic structure is probably more influenced by
Corynebacterium pseudotuberculosis is a bacterium which causes diseases such as caseous lymphadenitis in small ruminants, resulting in large-scale economic losses for agribusiness worldwide. Consequently, this bacterium including its transcriptional profile analysis has been the focus of various stu Integrative Computational Biology
Background Corynebacterium pseudotuberculosis is classified into two biovars, nitrate-negative biovar Ovis which is the etiologic agent of caseous lymphadenitis in small ruminants and nitrate-positive biovar Equi, which causes abscesses and ulcerative lymphangitis in equines. The aim of this study was to develop a quadruplex PCR assay that would allow simultaneous detection and biovar-typing of C. pseudotuberculosis. Methods In the present study, genomes of C. pseudotuberculosis strains were used to identify the genes involved in the nitrate reduction pathway to improve a species identification three-primer multiplex PCR assay. The nitrate reductase gene (narG) was included in the PCR assay along with the 16S, rpoB and pld genes to enhance the diagnosis of the multiplex PCR at biovar level. Results A novel quadruplex PCR assay for C. pseudotuberculosis species and biovar identification was developed. The results of the quadruplex PCR of 348 strains, 346 previously well-characterized clinical ...
Corynebacterium pseudotuberculosis biovar equi is the etiologic agent of ulcerative lymphangitis. To investigate proteins that could be related to the virulence of this pathogen, we combined an experimental passage process using a murine model and high-throughput proteomics with a mass spectrometry, data-independent acquisition (LC-MSE) approach to identify and quantify the proteins released into the supernatants of strain 258_equi. To our knowledge, this approach allowed characterization of the exoproteome of a C. pseudotuberculosis equi strain for the first time. Interestingly, the recovery of this strain from infected mouse spleens induced a change in its virulence potential, and it became more virulent in a second infection challenge. Proteomic screening performed from culture supernatant of the control and recovered conditions revealed 104 proteins that were differentially expressed between the two conditions. In this context, proteomic analysis of the recovered condition detected the induction of
The results of ROC analysis indicated that the IgG isotype ELISA was more accurate than the total antibody ELISA. The efficiency of the test was greatest when serum samples were run in a dilution series than when any single serum dilution was used. The ELISA is considered to be suitable for application in field studies of CLA in UK sheep.. ...
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
Nucleotide excision repair (NER) is a mechanism to recognize and repair bulky DNA damage caused by compounds, environmental carcinogens, and exposure to UV-light. In humans hereditary defects in the NER pathway are linked to at least three diseases: xeroderma pigmentosum (XP), Cockayne syndrome (CS), and trichothiodystrophy (TTD). The repair of damaged DNA involves at least 30 polypeptides within two different sub-pathways of NER known as transcription-coupled repair (TCR-NER) and global genome repair (GGR-NER). TCR refers to the expedited repair of lesions located in the actively transcribed strand of genes by RNA polymerase II (RNAP II). In GGR-NER the first step of damage recognition involves XPC-hHR23B complex together with XPE complex (in prokaryotes, uvrAB complex). The following steps of GGR-NER and TCR-NER are similar ...
A double antibody sandwich ELISA developed by ID-DLO, Lelystad to detect Corynebocterium pseudotuberculosis infection was used on 329 sheep from four pedigree Suffolk flocks in which clinical cases of caseous lymphadenitis (CLA) had occurred. At subsequent necropsy, typical CLA lesions were seen in 133 sheep, and the diagnosis was confirmed on culture. Lesions were most commonly seen in lungs (n = 46), parotid lymph nodes (n = 44), prescapular lymph nodes (n = 38) and mediastinal lymph nodes (n = 31). The sensitivity of the ELISA test for detecting culture-positive sheep was 0.88, while the specificity of the test was 0.55. The antibody ELISA detected 87.5 per cent of sheep that had CLA lesions restricted to internal organs only. It was concluded that the ELISA test has a valuable role in detecting sheep with both clinical and subclinical CLA.. ...
Corynebacterium pseudotuberculosis is a Gram-positive bacterium that belongs to the class Actinobacteria. This bacterium is a facultative intracellular pathogen that causes the disease caseous lymphadenitis (CL) in sheep and goats. During the process of infection by these bacteria are contained cells of the innate immune response, among which the main effectors defensives cells are neutrophils and macrophages. This work has been proposed with the aim of assessing the response of murine bone marrow monocyte-derived macrophages in in vitro infection with the CP13 strain of C. pseudotuberculosis - a knockout mutant for a gene related to the transportation of iron in Corynebacterium diphtheriae that generated good levels of protection against subsequent infections C. pseudotuberculosis - and observe the response profile generated by these cultures. This type of characterization allows the inference of the involvement of innate immune response against infections caused by this bacterium. So far, it ...
General Information: Strain FRC41 was isolated from the inguinal lymph node of a 12-year-old girl in France with necrotizing lymphadenitis. Corynebacterium pseudotuberculosis, an important animal pathogen, is the etiological agent of a disease that is commonly called caseous lymphadenitis (CLA) or cheesy gland. This disease is found in all the worlds major sheep and goat production areas, causing significant economic losses worldwide, mainly due to the reduction of wool, meat and milk yields, decreased reproductive efficiencies of affected animals and condemnation of carcasses and skins in abattoirs. In some cases, the infection produces few obvious clinical signs in the animal, remaining unrecognized until a post-mortem examination has been carried out and, making it difficult to obtain definitive data about prevalence of the disease. ...
There have been several reports of pigeon fever in the region. With this in mind, here are some facts about this condition in horses, including information about recognition, prevention, and treatment.. "Pigeon fever" got its name because the most common form of the disease involves swelling and abscessation of the pectoral region, which resembles a pigeons breast. The disease is not caused or carried by birds, but instead is the result of infection with a bacterium called Corynebacterium pseudotuberculosis. This pathogen can also affect other animals, including sheep and cattle, but the syndrome in horses is fairly unique to this species. It is most common in arid climates, but can definitely be found in Missouri and all throughout the United States, and its prevalence is increasing.. There are three forms of pigeon fever. The first is the most classic-the aforementioned abscesses in the chest (and potentially elsewhere on the body). While this can be painful and unsightly, most horses make a ...
External lumps and bumps in dairy goats have many causes, but most owners assume that all lumps are abscesses and that all abscesses are caused by Corynebacterium. Although Corynebacterium is a significant cause of external masses in goats, one should not assume that all lumps are abscesses or that all external masses occurring in a herd result from the same cause. Determining the correct origin of an external mass requires a detailed history, a thorough physical examination and aspiration or biopsy. Once a diagnosis has been made, an appropriate therapy can be chosen and a prognosis for recovery can be determined.. Abscesses caused by a wide variety of bacteria are the most common cause of external masses in dairy goats. Numerous bacteria live on the surface of healthy skin and mucous membranes and can be introduced into body tissues through small ulcers and puncture wounds. Coarse hay, grass awns, wood splinters, unsanitary injection needles and accidental trauma can introduce bacteria into ...
Risks for Caseous lymphadenitis , Caseous lymphadenitis treatments, recommended products for Caseous lymphadenitis , ways to prevent Caseous lymphadenitis , causes of Caseous lymphadenitis
Antisera against Actinobacillus seminis, Brucella ovis and Corynebacterium pseudotuberculosis were prepared in adult female goats. Specific immunofluorescence was observed in cultural smears of A seminis, B ovis and C pseudotuberculosis by the direct technique and in smears of A seminis also by the indirect technique. Individual organisms could be recognised. Specific fluorescence of A seminis was readily detected in semen. The results indicate that immunofluorescence may offer an effective method for rapidly and accurately diagnosing bacterial epididymitis in sheep, especially before epididymal lesions are palpable.. ...
Equine lymphangitis is an inflammation or swelling associated with impairment of the lymphatic system, particularly in a limb, in horses. It is most commonly a bacterial infection, although bacterial culture may be negative. Often referred to as fat/big leg disease, it is sometimes known as weed or Monday morning disease (not to be confused with the more common usage of MMD referring to exertional rhabdomyolysis or azoturia). This article refers mainly to sporadic lymphangitis. Ulcerative lymphangitis is referred to in passing, as it is managed in a similar manner. Epizootic lymphangitis is similar to glanders, but caused by the fungus Histoplasma farciminosum. Corynebacterium pseudotuberculosis has been cultured from some cases (particularly of ulcerative lymphangitis; however, in others, bacterial culture is negative. This may be because: a) The micro-organism responsible is difficult to culture (e.g. many Mycoplasma species). b) The organism has been effectively eliminated by the immune ...
When treating CLA *never burst the abscess to expel the fluid inside* this can be an extremely quick way of spreading the disease to others and to progressing the sheep to a visceral form of the disease. If it is necessary, shave the area round the abscess, isolate the sheep, make a small incision I the abscess, drain it fully, apply disinfectant to the area around it and thoroughly clean the area afterwards where the sheep has been penned. Never leave the fluid that is expelled on the floor or near water troughs or food sources. ...
Clinical signs include changes to the mucus membranes around the mouth, nose and coronary band of the foot. Generally the signs are more severe in sheep, than cattle. Symptoms notifiable are large numbers of sheep with lameness, high rectal temperatures, salivation, and lacrimation, ocular and nasal discharge. Incubation period of the virus is 4-12 days, followed by a fever (42 degrees C) and appear stiff or reluctant to move. Adopt arched back stance, neck extended, head lowered. Swelling on the face and ears, along with pulmonary oedema, sometimes causing breathing difficulties. Erosions may appear on lips, turning to ulcers. Profuse salivation and serous to mucopurulent nasal discharge. Reddening of the coronary band, and around the muzzle and mouth. Tongue may be swollen ...
24 May 2002 Shearing risks spreading CLACARE at shearing will help limit spread of sheep disease, caseous lymphadenitis. But development of a new blood tes
This case report describes a Yersinia pseudotuberculosis infection in an adult, female single pet goat kept on a dairy cattle farm. The doe showed the habitus of a chronically ill animal, with poor body condition and chronic diarrhoea, when presented at the clinic. The animal displayed a grave manifestation of the disease with severe enteritis and typhlocolitis, lymphadenitis and some up to 10 cm in diameter pyogranulomatous inflammation in the liver, spleen, pancreas, omasum and the intestinal lymph nodes. All other causative agents that might explain the described signs were ruled out (Corynebacterium pseudotuberculosis, Mycobacterium avium subspecies paratuberculosis, tuberculosis, tumorous alterations, foreign bodies, caprine arthritis encephalitis). Goats seem to be vulnerable for yersiniosis, but in contrast to this case report, in most cases, Yersinia enterocolitica, and not Y. pseudotuberculosis, can be proved as infectious cause of yersiniosis. ...
Enteropathogenic Yersinia species can infect many mammalian organs such as the small intestine, cecum, Peyers patches, liver, spleen, and lung and cause diseases that resemble a typhoid-like syndrome, as seen for other enteropathogens. We found that sublethal infection doses of Y. pseudotuberculosis gave rise to asymptomatic persistent infection in mice and identified the cecal lymphoid follicles as the primary site for colonization during persistence. Persistent Y. pseudotuberculosis is localized in the dome area, often in inflammatory lesions, as foci or as single cells, and also in neutrophil exudates in the cecal lumen. This new mouse model for bacterial persistence in cecum has potential as an investigative tool for deeper understanding of bacterial adaptation and host immune defense mechanisms during persistent infection. Here, we investigated the nature of the persistent infection established by Y. pseudotuberculosis in mouse cecal tissue using in vivo RNA-seq of bacteria during early ...
Yersinia pseudotuberculosis is a Gram-negative bacterium that causes Far East scarlet-like fever in humans, who occasionally get infected zoonotically, most often through the food-borne route. Animals are also infected by Y. pseudotuberculosis. The bacterium is urease positive. In animals, Y. pseudotuberculosis can cause tuberculosis-like symptoms, including localized tissue necrosis and granulomas in the spleen, liver, and lymph nodes. In humans, symptoms of Far East scarlet-like fever are similar to those of infection with Yersinia enterocolitica (fever and right-sided abdominal pain), except that the diarrheal component is often absent, which sometimes makes the resulting condition difficult to diagnose. Y. pseudotuberculosis infections can mimic appendicitis, especially in children and younger adults, and, in rare cases, the disease may cause skin complaints (erythema nodosum), joint stiffness and pain (reactive arthritis), or spread of bacteria to the blood (bacteremia). Far East ...
The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format. By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export. After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format. ...
Pierce, chase C.; Fauve, R M.; and Dubos, R, "Corynebacterial pseudotuberculosis in mice. I. Comparative susceptibility of mouse strains to experimental infection with coryne- bacterium kutscheri." (1964). Subject Strain Bibliography 1964. 1371 ...
Yersinia pseudotuberculosis ATCC ® 13979D-5™ Designation: Genomic DNA from NCTC 8580 (ATCC 13979) TypeStrain=False Application:
Yersinia pseudotuberculosis strain IP 31758. This strain is serotype 1b and was isolated in 1966 from the stools of a patient presenting with scarlet-like fever in the Primorski region of the former USSR. The strain was sent to the Institut Pasteur by Dr. Timofeeva (Antiplague Institute, Irkoutsk).Two libraries were constructed: a small insert library (4-5 kb) and a large insert library (10-12 kb) from which 11,818 and 49,665 reads were sequenced respectively. (NCBI BioProject: bp_list[1 ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Yersinia pseudotuberculosis is a soil- and water-borne enteropathogen. Numerous species are potential carriers of Y.pseudotuberculosis. The asymptomatic carriage can evolve into a fatal infection when the animals are stressed. In Human, different degrees of illness are observed, from fever and abdominal pain to septicemia.(From http://www.expasy.org/sprot/hamap/YERPS.html) (BacMap ...
associations are meant to be specific and meaningful, i.e. proteins jointly contribute to a shared function; this does not necessarily mean they are physically binding each other. ...
associations are meant to be specific and meaningful, i.e. proteins jointly contribute to a shared function; this does not necessarily mean they are physically binding each other. ...
This is Sweetie. Shes a Pygmy goat. One of Moms friends rescued Sweetie, but now Sweetie needs a home so shes come to live with us on our farm.. Some people Moms friend knows bought Sweetie at a sheep and goat auction at a place called a sale barn. People bring their animals to sale barns to sell them fast, but theyre not good places to buy. Animals sold at sale barns are often sick or theres something wrong with them. Its better to buy livestock from individuals or breeders. Heres why:. Even if an animal isnt sick before its sold through a sale barn, its exposed to disease on sale day. Some serious diseases you can bring home are strangles and flu in horses; hoof rot in cloven-hoofed animals; an incurable abscess-causing disease called caseous lymphadenitis in sheep and goats; and respiratory disease in livestock of all kinds.. In addition to sick animals, some people take other kinds of problem animals to sale barns to sell to unsuspecting buyers. Theyll sell sows that never get ...
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.
This post explores the Corynebacterium, a large group of mostly harmless bacteria that holds some surprises. Included is a demonstrated means of removal.
void:inDataset: http://aims.fao.org/aos/agrovoc/void.ttl#Agrovoc. Created: 2011-11-20T20:02:53Z. Last modified: 2011-11-20T20:02:53Z. skos:notation: 1913 ...
Dear collegues, I´d like to get to know people (scientists, work groups et c.), who work on any topic concerning the genera Corynebacterium or Brevibacterium. As I started my PhD studying Corynebacterium glutamicum (osmotic stress - amino acid production) last year I would be grateful to get in contact with as many people as possible sharing interest in this field of research. However, please don´t hesitate to contact me, if you´re working on the molecular biology level. My aim is to get to know problems you don´t read from in the literature or even share some problem solutions.... Thank you. If reply, please remove the nospam Yours, Hendrik Rönsch University of Cologne, Biochemistry Please visit my homepage: http://come.to/hendrik.roensch ...
This is the business view business. Lymphadenitis is swollen lymph nodes because of inflammation, usually occur as a result of exposure to a bacteria or virus. Less commonly, swollen lymph nodes are caused by cancer. Common areas where you might notice swollen lymph nodes include your neck...
Lymphadenitis is the inflammation or enlargement of a lymph node. Lymph nodes are small, ovoid nodules normally ranging in size from a few millimeters to 2 cm.
Lymphadenitis is the inflammation or enlargement of a lymph node. Lymph nodes are small, ovoid nodules normally ranging in size from a few millimeters to 2 cm.
Some adenine methyltransferases have been shown not only to protect specific DNA restriction sites from cleavage by a restriction endonuclease, but also to play a role in various bacterial processes and sometimes in bacterial virulence. This study focused on a type I restriction-modification system (designated yrmI) of Y. pseudotuberculosis. This system is composed of three adjacent genes which could potentially encode an N 6-adenine DNA methylase (YamA), an enzyme involved in site-specific recognition (YrsA) and a restriction endonuclease (YreA). Screening of 85 isolates of Y. pestis and Y. pseudotuberculosis indicated that the yrmI system has been lost by Y. pestis and that yamA (but not yrsA or yreA) is present in all Y. pseudotuberculosis strains tested, suggesting that it may be important at some stages of the epidemiological cycle of this species. To further investigate the role of yamA in Y. pseudotuberculosis survival, multiplication or virulence, a ΔyamA mutant of Y. pseudotuberculosis IP32953
Crohns disease is characterized by a defect in intestinal barrier function, where bacteria are considered the most important inflammation-driving factor. Enteric bacteria, including E. coli and Yersinia spp, affect tight junctions in enterocytes, but little is known about bacterial effects on the transcellular pathway. Our objective was to study the short-term effects of Y. pseudotuberculosis on uptake of nanoparticles across human villus epithelium. Monolayers of human colon epithelium-derived Caco-2 cells and biopsies of normal human ileum were studied after 2 h exposure to Y. pseudotuberculosis expressing (inv+) or lacking (inv-) the bacterial adhesion molecule, invasin. Transepithelial transport of fluorescent nanoparticles (markers of transcytosis) was quantified by flow cytometry, and mechanisms explored by using inhibitors of endocytosis. Epithelial expressions of beta 1-integrin and particle uptake pathways were studied by confocal microscopy. The paracellular pathway was assessed by ...
Academic Journals Database is a universal index of periodical literature covering basic research from all fields of knowledge, and is particularly strong in medical research, humanities and social sciences. Full-text from most of the articles is available. Academic Journals Database contains complete bibliographic citations, precise indexing, and informative abstracts for papers from a wide range of periodicals.
«Corynebacterium» Corynebacterium is a genus of Gram-positive, rod-shaped bacteria. They are widely distributed in nature and are mostly innocuous. Some are useful ...
Corynebacterium minutissimum symptoms, causes, diagnosis, and treatment information for Corynebacterium minutissimum (Erythrasma) with alternative diagnoses, full-text book chapters, misdiagnosis, research treatments, prevention, and prognosis.
Question - FNAC report shows granulomatous lymphadenitis. What is the conclusion of the findings? Treatment?. Ask a Doctor about diagnosis, treatment and medication for Tuberculosis, Ask an Internal Medicine Specialist
Lineage: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Yersinia; Yersinia pseudotuberculosis complex; Yersinia ...
Murahata, R I. and Zighelboim, J, "Systemic administration of corynebacterium parvum during sensitization to tumor alloantigen-modified response to rechallenge." (1980). Subject Strain Bibliography 1980. 606 ...
Corynebacterium pseudodiphtheriticum ATCC ® BAA-732™ Designation: Vitek #12653 TypeStrain=False Application: Quality control strain
Caruso, R. and Bonina, L. and Chimicatà, S. and Ianello, D. and Inferrera, C. and Mastroeni, P. (1978) Inibizione del tumore T8 di Guérin con il $Corynebacterium$ $parvum$: aspetti istomorfologici e di funzionalità macrofagica. Accademia Peloritana dei Pericolanti - Classe di Scienze Scienze Medico-Biologiche, LXVI (1). pp. 803-807. ...
Thermo Scientific™ Culti-Loops™ are ready-to-use QC organisms recommended for use in performance testing of media, stains, reagents and identification kits, and for the evaluation of bacteriological procedures.
Yersinia pseudotuberculosis serotype O:1b Phosphoadenosine phosphosulfate reductase (cysH) datasheet and description hight quality product and Backed by our Guarantee
Y. enterocolitica and Y. pseudotuberculosis are the third most common reported zoonoses after Campylobacter and Salmonella. Y. pseudotuberculosis has caused 10 outbreaks in Finland since 1997. The symptoms of yersiniosis include fever, right-sided abdominal pain and diarrhea. Post-infectious complications are common. Refrigeration is the most common modern food preservation method, however, as psychrotrophs, enteropathogenic Yersinia are able to grow at refrigerator temperatures. However, the mechanism behind psychrotrophy is still unknown. The aim of this study was to determine the expression levels of rstB, evgS and the genes coding for the σE- and σS-factors of Y. pseudotuberculosis IP 32953 at 30°C and after temperature drop to 5°C. Total RNA had been extracted before and 30 minutes, 3, and 7 hours after the temperature downshift for previously performed DNA microarray gene expression studies. Low temperature had been found to increase the expression levels of these genes in these ...
Isolated in 1966 from the stools of a patient presenting with Far East scarlet-like fever in the Primorski region of the former Soviet Union ...
Corynebacterium argentoratense is part of the human skin microbiota and is occasionally detected in the upper respiratory tract of patients suffering from tonsillitis. The complete DNA sequence of the type strain DSM 44202 comprises 2,031,902 bp, yielding the smallest genome sequenced thus far for a corynebacterium associated with humans. ...
After intradermal injection, BCG multiplies at the inoculation site, then spreads to regional nodes. A normal reaction is a red indurated area, which progresses to a local lesion that may ulcerate 2-3 weeks after vaccination. A crust is formed around this induration for 3-4 weeks. At 6-10 weeks, the crust falls off, leaving a flat 3 to 7 mm scar. Regional lymphadenopathy ,1 cm (95% in the axilla) is considered a normal reaction to the vaccine.4 This simple lymphadenitis occurs 63 days (range 16-87) after BCG vaccination5 and resolves spontaneously by 9 months.6 Lymphadenitis may be more common when BCG is given to infants ,6 months old compared with older children and adults.5 ...
The American Academy of Family Physicians (AAFP) says lymphadenitis is an inflammation of the lymph nodes, which dot the network of lymphatic_vessels and provide a collecting area for the immune_system cells that defend against invaders. Lymphadenitis is not uncommon in children. Causes can include bacterial, viral or other infection. AAFP says people can develop an infection following a cat scratch or bite. Treatment depends on the specific cause of infection and its bacterium. ...
Lymphadenitis is an infection of the lymph nodes (also called lymph glands). It is a complication of certain bacterial infections.
Although a large number of people do not consider pigeons a plague (generally people leans more to consider it a pest insects smaller animals), there is a movement pigeon which basically relies on the many diseases associated with organic waste from these animals, including salmonellosis, histoplasmosis, pneumonia, criptococcosis and pseudotuberculosis. The pigeons that we find in our day to day in the cities, are also carriers of half a hundred external ectoparasites of easy transmission both men and animals, such as ticks, bedbugs, mites and lice. These diseases can be transmitted by direct contact with droppings or simple inhalation. Architecture lovers also were added to the League pigeon, since these droppings are extremely harmful in buildings and monuments. Due to its high content of uric acid and fosfofico nature, excrement disintegrate cements, concrete and limestone, as well as also causes a greater deterioration in other materials. The conditions of any property are also affected by ...
However I took about 18 of these samples and streaked them onto blood agar. After 24hrs the growth rates were very mixed. 3 had excellent growth and were all IDed as corynebacterium spp. The other 15 had very little growth and only one of these had enough growth for a full ID. This one came off as Clostridium ...
Background Chorismate mutases of the AroQ homology class are widespread in the Bacteria and the Archaea. Many of these exist as domains that are fused with other aromatic-pathway catalytic domains. Among the monofunctional AroQ proteins, that from Erwinia herbicola was previously shown to have a cleavable signal peptide and located in the periplasmic compartment. Whether or not this might be unique to E. herbicola was unknown. Results The gene coding for the AroQ protein was cloned from Salmonella typhimurium, and the AroQ protein purified from both S. typhimurium and Pseudomonas aeruginosa was shown to have a periplasmic location. The periplasmic chorismate mutases (denoted *AroQ) are shown to be a distinct subclass of AroQ, being about twice the size of cytoplasmic AroQ proteins. The increased size is due to a carboxy-terminal extension of unknown function. In addition, a so-far novel aromatic aminotransferase was shown to be present in the periplasm of P. aeruginosa. Conclusions Our analysis has
Diphtheria toxin (DT) is produced by toxigenic strains of the human pathogen Corynebacterium diphtheriae as well as zoonotic C. ulcerans and C. pseudotuberculosis. Toxigenic strains may cause severe respiratory diphtheria, myocarditis, neurological damage or cutaneous diphtheria. The DT encoding tox gene is located in a mobile genomic region and tox variability between C. diphtheriae and C. ulcerans has been postulated based on sequences of a few isolates. In contrast, species-specific sequence analysis of the diphtheria toxin repressor gene (dtxR), occurring both in toxigenic and non-toxigenic Corynebacterium species, has not been done yet. We used whole genome sequencing data from 91 toxigenic and 46 non-toxigenic isolates of different pathogenic Corynebacterium species of animal or human origin to elucidate differences in extracted DT, DtxR and tox-surrounding genetic elements by a phylogenetic analysis in a large sample set. Sequences of both DT and DtxR, extracted from whole genome sequencing data,
Looking for Corynebacterium parvum? Find out information about Corynebacterium parvum. A genus of gram-positive, straight or slightly curved rods in the coryneform group of bacteria; club-shaped swellings are common; includes human and animal... Explanation of Corynebacterium parvum
Yersinia pseudotuberculosis YopB and YopD proteins are essential for translocation of Yop effector proteins into the target cell cytosol. YopB is suggested to mediate pore formation in the target cell plasma membrane, allowing translocation of Yop effector proteins, although the function of YopD is unclear. To investigate the role in translocation for YopD, a mutant strain in Y. pseudotuberculosis was constructed containing an in frame deletion of essentially the entire yopD gene. As shown recently for the Y. pestis YopD protein, we found that the in vitro low calcium response controlling virulence gene expression was negatively regulated by YopD. This yopD null mutant (YPIII/pIB621) was also non-cytotoxic towards HeLa cell monolayers, supporting the role for YopD in the translocation process. Although other constituents of the Yersinia translocase apparatus (YopB, YopK and YopN) are not translocated into the host cell cytosol, fractionation of infected HeLa cells allowed us to identify the ...
A diferenza dos produtos dos xenes ysc e yop mencionados antes, as Yops que actúan directamente sobre as células hóspede para causar efectos citopatolóxicos ("Yops efectoras") están codificadas por xenes pYV que están fóra da rexión central ou core.[5] A única excepción é LcrV, que tamén se chama "Yop versátil" polos dous papeis que ten como Yop efectora e reguladora.[5] A función combinada destas Yops efectoras permite á bacteria resistir a súa internalización en células intestinais inmunitarias e evadirse das accións bactericidas dos neutrófilos e macrófagos. Dentro da bacteria, estas Yops únense a proteínas Sycs codificadas no pYV (que son chaperonas de Yop específicas), que impiden a interacción prematura con outras proteínas e guían as Yops a un aparato secretorio de tipo III.[9] Ademais do complexo Syc-Yop, as Yops están tamén etiquetadas para a secreción de tipo III polos primeiros 60 nucleótidos do seu transcrito de ARNm correspondente ou polos ...
Free Online Library: Nosocomial endocarditis caused by Corynebacterium amycolatum and other nondiphtheriae corynebacteria. (Dispatches). by Emerging Infectious Diseases; Health, general Cross infection Causes of Drug resistance in microorganisms Research Endocarditis Microbial drug resistance Nosocomial infections Pathogenic microorganisms
Cervical lymphadenitis is common in childhood. The incidence is difficult to ascertain because it is usually caused by a viral upper respiratory infection (URI) and is self-limited.The etiology, pathophysiology, and clinical manifestations of cervica
The genus of Gram positive bacilli including Corynebacterium diphtheriae, the cause of diphtheria in humans. Genus also includes C. minutissimum, the cause of erythrasma in humans and the diphtheroids which are commensal corynebacteria making up part of the human respiratory tract normal flora.. ...
General Information: Environmental bacterium that causes gastrointestinal disease. This organism was first isolated in 1883 by Malassez and Vignal and is termed pseudotuberculosis since it causes lesions in the lung that are similar to those observed during tuberculosis infection. It is ubiquitous in the environment and is a food and waterborne pathogen that affects animals as well as humans by causing gastroenteritis like Yersinia enterocolitica. ...
Genomics: Corynebacterium diphtheriae: chromosome 2,488,635 bp; 2320 predicted ORFs (Cerdeno-Tarraga et al. 2003) Cell morphology: Rod-shaped cells; irregular, club-shaped ( Coryne), or V-shaped...
Read "Whole-genome sequence analysis of G3 and G14 equine group A rotaviruses isolated in the late 1990s and 2009-2010, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Centers RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.. ...
Another name for Infected Lymph Nodes is Lymphadenitis. Notify your doctor if you have lymphadenitis and any of the following: * Red streaks in the skin ...
What is the best treatment for swelling of private parts - Does Tb neck lymphadenitis spread to the genital area during 6th month treatment? Can TB lymphadenitis cause lymph node swelling on labia majora? Noy usually. However, any lymph node infection has the ptoential to travel throughout the body, the genital area is not common: get screened for opportinitistic infections and STDs.
Die Universität zu Köln ist eine Exzellenzuniversität mit dem klassischen Fächerspektrum einer Volluniversität. Als eine der größen Hochschulen Europas arbeitet sie in Forschung und Lehre auch international auf höchstem Niveau.
Hemicell minimizes production and economic losses caused by the FIIR from β-mannans, allowing more energy to be available for growth and performance.
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Author: S. MATHAVI, A.V. RAGHAVENDRA RAO, A. KAVITHA, G. SASIKALA, INDRA PRIYADHARSINI. Category: Microbiology. [Download PDF]. Abstract:. Introduction: Coryneform or the nondiphtherial Corynebacterium species remains a neglected group as contaminants. Theseorganisms havebeen associated with invasive disease, particularly in immunocompromised patients. Species like Corynebacterium amycolatum, Corynebacterium jeikeium , Corynebacterium minutissimum and Corynebacterium urealyticum are reported with increasing frequency. An alarming rate of antibiotic resistance is also documented among such organisms. Aim and Objective: This study was done to find out the various species of clinically relevant Coryneforms and to determine their antibiogram.. Materials and Methods: A total of 857 clinical samples (Pus, wound swab, urine, blood, sputum and catheter tips) received in the Microbiology department during January 2013 to October 2013 were included in the study. They were subjected to Grams staining and ...
Y. pseudotuberculosis and Y. enterocolitica both infect the intestines of mammals through the fecal-oral route (contaminated food and water) and rarely is deadly. Y. pestis, on the other hand, is transmitted subcutaneously through a bite of an infected flea or rat (bubonic), but can also be transmitted by air (especially during pandemics of the disease). More specifically, fleas become infected after taking blood meals from septicemic animals and becoming infected themselves. Y. pestis grows in the midgut and eventually blocks the proventriculus, starving the flea for blood. The insects attempt to feed more often but end up giving back infected blood into the wound of the bite. The flea eventually dies, presumably from starvation and dehydration. On an interesting note, when the temperatures get higher fleas do not have their proventriculus blocked, and only those that are blocked can transmit the disease. When held at 30oC, fleas survive infections in an unblocked state, possibly leading at an ...
Page contains details about Corynebacterium glutamicum catalase-gold nanoparticles nanostructured material . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
The duration of treatment for mesenteric adenitis, also known as lymphadenitis, is influenced by the cause for the lymphadenitis and the severity of the disease, according to Medscape. Sometimes no...
Many studies have highlighted the importance of body site and individuality in shaping the composition of the human skin microbiome, but we still have a poor understanding of how extrinsic (e.g., lifestyle) and intrinsic (e.g., age) factors influence its composition. We characterized the bacterial microbiomes of North American volunteers at four skin sites and the mouth. We also collected extensive subject metadata and measured several... ...
Corbeil, LE; Morrissey, JF; Léguillette, R (October 2016). "Is Corynebacterium pseudotuberculosis infection (pigeon fever) in ... "Frequency of Corynebacterium pseudotuberculosis infection in horses across the United States during a 10-year period". Journal ... "Experimental transmission of Corynebacterium pseudotuberculosis biovar equi in horses by house flies". Journal of veterinary ... caused by the Gram-positive bacteria Corynebacterium pseudotuberculosis biovar equi. Infected horses commonly have swelling in ...
Corynebacterium pseudotuberculosis has been cultured from some cases (particularly of ulcerative lymphangitis; however, in ... However, intracellular organisms such as Corynebacterium pseudotuberculosis will be susceptible to certain antibiotics in vitro ...
Corynebacterium pseudo tuberculosis I19 as a case study. Journal of Microbiological Methods, 86(2), 218-223. doi: 10.1016/j. ... pseudotuberculosis was assembled twice: once using a classical reference genome approach, and once using a hybrid approach. The ...
... (CLA) is an infectious disease caused by the bacterium Corynebacterium pseudotuberculosis found mostly in ...
Corynebacterium ulcerans Coxiella burnetii or Q fever Plesiomonas shigelloides In addition to disease caused by direct ... including O1 and non-O1 Vibrio parahaemolyticus Vibrio vulnificus Yersinia enterocolitica and Yersinia pseudotuberculosis Less ...
Corynebacterium pseudotuberculosis (causative agent of pigeon fever), equine influenza virus, or equine herpes virus type 1, or ...
Corynebacterium glutamicum MeSH B03.510.460.400.400.200.600 --- Corynebacterium pseudotuberculosis MeSH B03.510.460.400.400.200 ... Corynebacterium glutamicum MeSH B03.510.024.049.180.600 --- Corynebacterium pseudotuberculosis MeSH B03.510.024.049.180.625 ... Corynebacterium MeSH B03.510.024.049.180.100 --- Brevibacterium flavum MeSH B03.510.024.049.180.150 --- Corynebacterium ... Corynebacterium MeSH B03.510.460.400.400.200.150 --- Corynebacterium diphtheriae MeSH B03.510.460.400.400.200.300 --- ...
Several species cause disease in animals, most notably C. pseudotuberculosis, which causes the disease caseous lymphadenitis, ... afermentans Corynebacterium auris Corynebacterium pseudodiphtheriticum Corynebacterium propinquum Corynebacterium uropygiale ... Corynebacterium xerosis and Corynebacterium striatum Corynebacterium minutissimum Corynebacterium amycolatum Corynebacterium ... lipophilum Corynebacterium accolens Corynebacterium macginleyi CDC coryneform groups F-1 and G Corynebacterium bovis ...
Corynebacterium ulcerans e Corynebacterium pseudotuberculosis que son tanben la causa d'unas formas de difteria[17] d'origina ... C. pseudotuberculosis provòca tanben una zoonòsi, transmesa per las cabras[18]. Epidemiologia[modificar , modificar la font]. ... La difteria es causada per de socas toxigènas du bacil Corynebacterium diphtheriae de biotipes gravis, mitis o intermedius. La ... Toxigenic Corynebacterium ulcerans in a fatal human case and her feline contacts, France, March 2014. », Eurosurveillance,‎ 11 ...
Clostridium tetani Corynebacterium Corynebacterium diphtheriae Corynebacterium fusiforme Coxiella burnetii Ehrlichia ... Wolbachia Yersinia Yersinia enterocolitica Yersinia pestis Yersinia pseudotuberculosis List of bacterial orders List of ...
... corynebacterium infections MeSH C01.252.410.040.246.388 --- diphtheria MeSH C01.252.410.040.246.430 --- erythrasma MeSH C01.252 ... yersinia pseudotuberculosis infections MeSH C01.252.400.349 --- flavobacteriaceae infections MeSH C01.252.400.368 --- ...
Yersinia pseudotuberculosis infection Yersinia pseudotuberculosis Yersiniosis Yersinia enterocolitica Yellow fever Yellow fever ... Corynebacterium diphtheriae Diphyllobothriasis Diphyllobothrium Dracunculiasis Dracunculus medinensis Ebola hemorrhagic fever ...
2005). "Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid- ... 2004). "Insights into the evolution of Yersinia pestis through whole-genome comparison with Yersinia pseudotuberculosis". Proc ... 2003). "The complete genome sequence and analysis of Corynebacterium diphtheriae NCTC13129". Nucleic Acids Res. 31 (22): 6516- ... complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium ...
Corynebacterium diphtheriae *Diphtheria. *Corynebacterium minutissimum *Erythrasma. *Corynebacterium jeikeium *Group JK ...
Ionfhabhtú Yersinia pseudotuberculosis. Yersinia pseudotuberculosis. Yersiniosis. Yersinia enterocolitica. Yellow fever. Yellow ... Corynebacterium diphtheriae. Diphyllobothriasis. Diphyllobothrium. Dracunculiasis. Dracunculus medinensis. Ebola hemorrhagic ...
Organism: CORYNEBACTERIUM PSEUDOTUBERCULOSIS 1002 If you have problems or comments... Back to PBIL home page ... Organism: CORYNEBACTERIUM PSEUDOTUBERCULOSIS 1002 15. CORP1_1_PE1010 RecName: Full=Pup--protein ligase; EC=6.3.2 n2;AltName: ... Organism: CORYNEBACTERIUM PSEUDOTUBERCULOSIS 1002 8. CORP1_1_PE1004 SubName: Full=Xaa-Pro amino peptidase; (CORP1_1.PE1004). ... Organism: CORYNEBACTERIUM PSEUDOTUBERCULOSIS 1002 10. CORP1_1_PE1006 SubName: Full=Sec-in dependent twin-arginine translocase ...
Glycolysis / Gluconeogenesis - Corynebacterium pseudotuberculosis 267 [ Pathway menu , Organism menu , Pathway entry , Download ...
Sulfur metabolism - Corynebacterium pseudotuberculosis Cp162 [ Pathway menu , Organism menu , Pathway entry , Download KGML , ...
Carbon metabolism is the most basic aspect of life. This map presents an overall view of central carbon metabolism, where the number of carbons is shown for each compound denoted by a circle, excluding a cofactor (CoA, CoM, THF, or THMPT) that is replaced by an asterisk. The map contains carbon utilization pathways of glycolysis (map00010), pentose phosphate pathway (map00030), and citrate cycle (map00020), and six known carbon fixation pathways (map00710 and map00720) as well as some pathways of methane metabolism (map00680). The six carbon fixation pathways are: (1) reductive pentose phosphate cycle (Calvin cycle) in plants and cyanobacteria that perform oxygenic photosynthesis, (2) reductive citrate cycle in photosynthetic green sulfur bacteria and some chemolithoautotrophs, (3) 3-hydroxypropionate bi-cycle in photosynthetic green nonsulfur bacteria, two variants of 4-hydroxybutyrate pathways in Crenarchaeota called (4) hydroxypropionate-hydroxybutyrate cycle and (5) ...
Peptidoglycan biosynthesis - Corynebacterium pseudotuberculosis I19 [ Pathway menu , Organism menu , Pathway entry , Download ...
Carbon metabolism - Corynebacterium pseudotuberculosis P54B96 [ Pathway menu , Organism menu , Pathway entry , Hide module list ...
Peptidoglycan biosynthesis - Corynebacterium pseudotuberculosis C231 [ Pathway menu , Organism menu , Pathway entry , Download ...
... Kate L. Hepworth-Warren,1 Beatrice T ... Corynebacterium pseudotuberculosis biovar equi is the causative agent of "pigeon fever," or "dryland distemper" in horses. The ...
Information about Corynebacterium pseudotuberculosis infection (pigeon fever); signs, diagnosis, treatment options, and ... Corynebacterium pseudotuberculosis is a soil-borne anaerobic bacterium. has a thick lipid coat that lies outside its cell wall ... In horses Corynebacterium pseudotuberculosis causes infection of lower limbs, internal organs (lungs, liver, and kidneys), ... 6,7In the southwestern USA, Corynebacterium pseudotuberculosis infection is seasonal which peaks in the summer and fall. 1 ...
Carbon metabolism - Corynebacterium pseudotuberculosis CIP 52.97 [ Pathway menu , Organism menu , Pathway entry , Hide module ...
Metabolic pathways - Corynebacterium pseudotuberculosis CIP 52.97 [ Pathway menu , Organism menu , Pathway entry , Hide module ...
Nucleotide excision repair - Corynebacterium pseudotuberculosis I19 [ Pathway menu , Organism menu , Pathway entry , Download ...
Citrate cycle (TCA cycle) - Corynebacterium pseudotuberculosis I19 [ Pathway menu , Organism menu , Pathway entry , Download ...
Homologous recombination - Corynebacterium pseudotuberculosis 1/06-A [ Pathway menu , Organism menu , Pathway entry , Download ...
Pentose phosphate pathway - Corynebacterium pseudotuberculosis 42/02-A [ Pathway menu , Organism menu , Pathway entry , ...
Ubiquinone and other terpenoid-quinone biosynthesis - Corynebacterium pseudotuberculosis 316 [ Pathway menu , Organism menu , ...
Corynebacterium pseudotuberculosis is a bacterium which causes diseases such as caseous lymphadenitis in small ruminants, ... The core stimulon of Corynebacterium pseudotuberculosis strain 1002 identified using ab initio methodologies A. C. Pinto, R. T ... Corynebacterium pseudotuberculosis is a bacterium which causes diseases such as caseous lymphadenitis in small ruminants, ... We sequenced the cDNA of Corynebacterium pseudotuberculosis strain 1002, using the SOLiD V3 system, under the following ...
But this very interesting article from Israel describes a corynebacterium pseudotuberculosis outbreak in a dairy cattle herd ... Mastitis is a well-known sign for a corynebacterium pseudotuberculosis infection. ... CORYNEBACTERIUM PSEUDOTUBERCULOSIS OUTBREAK CAUSES SKIN LESIONS AND MASTITIS Mastitis is a well-known sign for a ... But this very interesting article from Israel describes a corynebacterium pseudotuberculosis outbreak in a dairy cattle herd ...
Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis, a debilitating chronic disease of sheep and ... Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis, a debilitating chronic disease of sheep and ... Baird, G. J., and Fontaine, M. C. (2007).Corynebacterium pseudotuberculosis and its role in ovine caseous lymphadenitis. ... Dorella, F. A., Pacheco, L. G. C., Oliveira, S. C., Miyoshi, A., and Azevedo, V. (2006). Corynebacterium pseudotuberculosis: ...
To our knowledge, this approach allowed characterization of the exoproteome of a C. pseudotuberculosis equi strain for the ... To our knowledge, this approach allowed characterization of the exoproteome of a C. pseudotuberculosis equi strain for the ... Moreover, this study enhances our understanding of the factors that may influence the pathogenesis of C. pseudotuberculosis. ... pseudotuberculosis. Collectively, bacterial pathogenesis-related proteins were identified that contribute to the processes of ...
Corynebacterium pseudotuberculosis (Cp) is the causative agent of caseous lymphadenitis (CLA), a chronic bacterial disease of ... Corynebacterium pseudotuberculosis (Cp) is the causative agent of caseous lymphadenitis (CLA), a chronic bacterial disease of ... Cell invasion and intracellular survival of corynebacterium pseudotuberculosis. 2011, University of Zurich, Vetsuisse Faculty. ...
Serological and allergic study of the course of experimental Corynebacterium pseudotuberculosis infection in goats ... Corynebacterium pseudotuberculosis infection in goats. IV. Course of the infection in two recently infected goat herds. Acta ... Corynebacterium pseudotuberculosis infection in goats in Norway, with special reference to diagnosis, natural and experimental ... Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-gamma production, ...
In this work, we present the resequencing of Corynebacterium pseudotuberculosis strain 1002 (Cp1002). Cp1002 was the first ... The new assembly of C. pseudotuberculosis was deposited in GenBank under the accession no. CP012837. ... Studies have detected mis-assemblies in genomes of the species Corynebacterium pseudotuberculosis. These new discover have been ... Corynebacterium pseudotuberculosis strain 1002 (first assembly). Cp1002B: Corynebacterium pseudotuberculosis strain 1002 (new ...
This work represents the first report of Corynebacterium pseudotuberculosis from caseous lymphadenitis lesions in Black ... To our knowledge, this is the first characterization of C. pseudotuberculosis from caseous lymphadenitis lesions in Black ... Furthermore, swine isolates were compared with C. pseudotuberculosis from caprines and PFGE patterns also showed high ... Macrorestriction patterns obtained by Pulsed Field Gel Electrophoresis (PFGE) grouped the C. pseudotuberculosis in two clusters ...
There is paucity of information on the proinflammatory cytokine (IL-1β and IL-6) responses due to C. pseudotuberculosis and ... The concentration of IL-6 was significantly increased (p,0.05) in weeks five, six, seven and eight in C. pseudotuberculosis ... Group C was inoculated with 2 mL 1×109 colony forming unit (cfu) of live C. pseudotuberculosis intradermally. Blood was ... Therefore, this study was conducted to evaluate the immunogenicity of C. pseudotuberculosis and MAs. Twelve clinically healthy ...
  • C. pseudotuberculosis can become endemic in a herd and disease eradication is problematic due to poor response to antibiotic therapy, organism ability to persist in the environment and difficulties in detecting subclinically infected animals (Al-gaabary et al 2009). (conicyt.cl)
  • Very recently, we showed for the first time the presence of PQO activity in a prokaryotic organism apart from E. coli and described the isolation, purification, and biochemical analysis of the PQO enzyme from Corynebacterium glutamicum ( 59 ). (asm.org)
  • However, intracellular organisms such as Corynebacterium pseudotuberculosis will be susceptible to certain antibiotics in vitro that are not effective for the specific organism in the horse. (wikipedia.org)
  • But this very interesting article from Israel describes a corynebacterium pseudotuberculosis outbreak in a dairy cattle herd causing both ulcerative granulomatous skin lesions and mastitis. (vetcontact.com)
  • C. pseudotuberculosis organisms that were isolated from the ulcerative granulomatous lesions and from milk samples failed to reduce nitrate. (vetcontact.com)
  • Use of a real-time polymerase chain reaction-based fluorogenic 5' nuclease assay to evaluate insect vectors of Corynebacterium pseudotuberculosis infections in horses. (semanticscholar.org)
  • Corynebacterium pseudotuberculosis was isolated from the facial wounds in both horses. (elsevier.com)
  • 3 Once established in the host, Corynebacterium pseudotuberculosis evades the immune system with relative ease, causing chronic infections that persist for the life of the animal. (gopetsamerica.com)
  • Pseudotuberculosis is characterised by the formation of encapsulated abscesses containing thick, caseous pus, most frequently within peripheral lymph nodes, but also within internal lymph nodes and organs ( Fontaine et al 2006 ). (conicyt.cl)
  • Serum Hp concentration in goats challenged with C. pseudotuberculosis (inoculated with 1x10 9 cfu subcutaneously) showed a significant increase, 5 fold in males (0.98 ± 0.12 mg/ml) and 3 fold in females (0.66 ± 0.12 mg/ml) compared to the control (0.2 ± 0.02 mg/ml). (biomedcentral.com)
  • Moxoto goats (n=17) were experimentally inoculated intradermally with broth culture containing varying numbers of living Corynebacterium pseudotuberculosis and its exotoxin, five goats with broth culture supernatant containing solely crude exotoxin and five goats with washed C. pseudotuberculosis suspended in physiological saline. (elsevier.com)
  • A novel comparative genomics analysis for common drug and vaccine targets in Corynebacterium pseudotuberculosis and other CMN group of human pathogens. (crcpress.com)