Corynebacterium glutamicum. Medical search

A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.

A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.

Infections with bacteria of the genus CORYNEBACTERIUM.

A species of gram-positive, asporogenous bacteria in which three cultural types are recognized. These types (gravis, intermedius, and mitis) were originally given in accordance with the clinical severity of the cases from which the different strains were most frequently isolated. This species is the causative agent of DIPHTHERIA.

Proteins found in any species of bacterium.

A species of gram-positive, asporogenous bacteria that was originally isolated from necrotic areas in the kidney of a sheep. It may cause ulcerative lymphangitis, abscesses, and other chronic purulent infections in sheep, horses, and other warm-blooded animals. Human disease may form from contact with infected animals.

Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.

Methods and techniques used to genetically modify cells' biosynthetic product output and develop conditions for growing the cells as BIOREACTORS.

A bacteria isolated from normal skin, intestinal contents, wounds, blood, pus, and soft tissue abscesses. It is a common contaminant of clinical specimens, presumably from the skin of patients or attendants.

The functional hereditary units of BACTERIA.

A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Deoxyribonucleic acid that makes up the genetic material of bacteria.

A gram-positive organism found in dairy products, fresh and salt water, marine organisms, insects, and decaying organic matter.

A dicarboxylic acid ketone that is an important metabolic intermediate of the CITRIC ACID CYCLE. It can be converted to ASPARTIC ACID by ASPARTATE TRANSAMINASE.

Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.

A naturally occurring compound that has been of interest for its role in osmoregulation. As a drug, betaine hydrochloride has been used as a source of hydrochloric acid in the treatment of hypochlorhydria. Betaine has also been used in the treatment of liver disorders, for hyperkalemia, for homocystinuria, and for gastrointestinal disturbances. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1341)

In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.

An essential amino acid. It is often added to animal feed.

The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.

A water-soluble, colorless crystal with an acid taste that is used as a chemical intermediate, in medicine, the manufacture of lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and a neutralizing agent. (Hawley's Condensed Chemical Dictionary, 12th ed, p1099; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1851)

A localized infection of mucous membranes or skin caused by toxigenic strains of CORYNEBACTERIUM DIPHTHERIAE. It is characterized by the presence of a pseudomembrane at the site of infection. DIPHTHERIA TOXIN, produced by C. diphtheriae, can cause myocarditis, polyneuritis, and other systemic toxic effects.

A species of CORYNEBACTERIUM isolated from abscesses of warm-blooded animals.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.

Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.

Salts and esters of gentisic acid.

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Sets of enzymatic reactions occurring in organisms and that form biochemicals by making new covalent bonds.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

An enzyme that catalyzes the reduction of aspartic beta-semialdehyde to homoserine, which is the branch point in biosynthesis of methionine, lysine, threonine and leucine from aspartic acid. EC 1.1.1.3.

The genetic complement of a BACTERIA as represented in its DNA.

Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.

A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.

Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.

An oxidative decarboxylation process that converts GLUCOSE-6-PHOSPHATE to D-ribose-5-phosphate via 6-phosphogluconate. The pentose product is used in the biosynthesis of NUCLEIC ACIDS. The generated energy is stored in the form of NADPH. This pathway is prominent in tissues which are active in the synthesis of FATTY ACIDS and STEROIDS.

Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.

The use of genetic methodologies to improve functional capacities of an organism rather than to treat disease.

An enzyme that catalyzes the conversion of prephenate to phenylpyruvate with the elimination of water and carbon dioxide. In the enteric bacteria this enzyme also possesses chorismate mutase activity, thereby catalyzing the first two steps in the biosynthesis of phenylalanine. EC 4.2.1.51.

An enzyme that catalyzes the oxidation of (R)-2,3-dihydroxy-3-methylbutanoate to (S)-2-hydroxy-2-methyl-3-oxobutanoate in the presence of NADP. It is involved in the biosynthesis of VALINE; LEUCINE; ISOLEUCINE; pentothenate and COENZYME A. This enzyme was formerly classified as EC 1.1.1.89.

A group of compounds that are derivatives of heptanedioic acid with the general formula R-C7H11O4.

Polysaccharides composed of repeating galactose units. They can consist of branched or unbranched chains in any linkages.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

An essential branched-chain aliphatic amino acid found in many proteins. It is an isomer of LEUCINE. It is important in hemoglobin synthesis and regulation of blood sugar and energy levels.

An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.

An important enzyme in the glyoxylic acid cycle which reversibly catalyzes the synthesis of L-malate from acetyl-CoA and glyoxylate. This enzyme was formerly listed as EC 4.1.3.2.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.

A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.

A branched-chain essential amino acid that has stimulant activity. It promotes muscle growth and tissue repair. It is a precursor in the penicillin biosynthetic pathway.

A non-essential amino acid naturally occurring in the L-form. Glutamic acid is the most common excitatory neurotransmitter in the CENTRAL NERVOUS SYSTEM.

In eukaryotes, a genetic unit consisting of a noncontiguous group of genes under the control of a single regulator gene. In bacteria, regulons are global regulatory systems involved in the interplay of pleiotropic regulatory domains and consist of several OPERONS.

An isomerase that catalyzes the conversion of chorismic acid to prephenic acid. EC 5.4.99.5.

Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.

An aldotriose which is an important intermediate in glycolysis and in tryptophan biosynthesis.

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

A species of METHYLOPHILUS which is motile by single flagella. In addition to growth on methanol as a sole carbon source, growth also occurs on glucose. (From Bergey's Manual of Determinative Bacteriology, 9th ed)

A key enzyme in the glyoxylate cycle. It catalyzes the conversion of isocitrate to succinate and glyoxylate. EC 4.1.3.1.

The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.

An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.

Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.

A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)

The bacterial sugar phosphotransferase system (PTS) that catalyzes the transfer of the phosphoryl group from phosphoenolpyruvate to its sugar substrates (the PTS sugars) concomitant with the translocation of these sugars across the bacterial membrane. The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. The PTS has also been implicated in the induction of synthesis of some catabolic enzyme systems required for the utilization of sugars that are not substrates of the PTS as well as the regulation of the activity of ADENYLYL CYCLASES. EC 2.7.1.-.

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

An intermediate compound in the metabolism of carbohydrates, proteins, and fats. In thiamine deficiency, its oxidation is retarded and it accumulates in the tissues, especially in nervous structures. (From Stedman, 26th ed)

An enzyme that catalyzes the synthesis of acetylphosphate from acetyl-CoA and inorganic phosphate. Acetylphosphate serves as a high-energy phosphate compound. EC 2.3.1.8.

Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.

A flavoprotein enzyme that catalyzes the formation of acetolactate from 2 moles of PYRUVATE in the biosynthesis of VALINE and the formation of acetohydroxybutyrate from pyruvate and alpha-ketobutyrate in the biosynthesis of ISOLEUCINE. This enzyme was formerly listed as EC 4.1.3.18.

Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC 2.4.1.32, EC 2.4.1.48, EC 2.4.1.54, and EC 2.4.1.57.

Evolutionary process of amino acid biosynthesis in Corynebacterium at the whole genome level. (1/403)

Corynebacterium glutamicum, which is the closest relative of Corynebacterium efficiens, is widely used for the large scale production of many kinds of amino acids, particularly glutamic acid and lysine, by fermentation. Corynebacterium diphtheriae, which is well known as a human pathogen, is also closely related to these two species of Corynebacteria, but it lacks such productivity of amino acids. It is an important and interesting question to ask how those closely related bacterial species have undergone such significant functional differentiation in amino acid biosynthesis. The main purpose of the present study is to clarify the evolutionary process of functional differentiation among the three species of Corynebacteria by conducting a comparative analysis of genome sequences. When Mycobacterium and Streptomyces were used as out groups, our comparative study suggested that the common ancestor of Corynebacteria already possessed almost all of the gene sets necessary for amino acid production. However, C. diphtheriae was found to have lost the genes responsible for amino acid production. Moreover, we found that the common ancestor of C. efficiens and C. glutamicum have acquired some of genes responsible for amino acid production by horizontal gene transfer. Thus, we conclude that the evolutionary events of gene loss and horizontal gene transfer must have been responsible for functional differentiation in amino acid biosynthesis of the three species of Corynebacteria. (+info)

Purification and characterization of O-Acetylserine sulfhydrylase of Corynebacterium glutamicum. (2/403)

We highly purified O-acetylserine sulfhydrylase from the glutamate-producing bacterium Corynebacterium glutamicum. The molecular mass of the purified enzyme was 34,500 as determined by SDS-polyacrylamide gel electrophoresis, and 70,800 as determined by gel filtration chromatography. It had an apparent Km of 7.0 mM for O-acetylserine and a Vmax of 435 micromol min-1 (mg x protein)-1. This is the first report of the cysteine biosynthetic enzyme of C. glutamicum in purified form. (+info)

BetP of Corynebacterium glutamicum, a transporter with three different functions: betaine transport, osmosensing, and osmoregulation. (3/403)

In order to circumvent deleterious effects of hypo- and hyperosmotic conditions in its environment, Corynebacterium glutamicum has developed a number of mechanisms to counteract osmotic stress. The first response to an osmotic upshift is the activation of uptake mechanisms for the compatible solutes betaine, proline, or ectoine, namely BetP, EctP, ProP, LcoP and PutP. BetP, the most important uptake system responds to osmotic stress by regulation at the level of both protein activity and gene expression. BetP was shown to harbor three different properties, i.e. catalytic activity (betaine transport), sensing of appropriate stimuli (osmosensing) and signal transduction to the catalytic part of the carrier protein which adapts its activity to the extent of osmotic stress (osmoregulation). BetP is comprised of 12 transmembrane segments and carries N- and C-terminal domains, which are involved in osmosensing and/or osmoregulation. Recent results on molecular properties of these domains indicate the significance of particular amino acids within the terminal 25 amino acids of the C-terminal domain of BetP for the process of osmosensing and osmoregulation. (+info)

Acyl-CoA carboxylases (accD2 and accD3), together with a unique polyketide synthase (Cg-pks), are key to mycolic acid biosynthesis in Corynebacterianeae such as Corynebacterium glutamicum and Mycobacterium tuberculosis. (4/403)

The Corynebacterianeae such as Corynebacterium glutamicum and Mycobacterium tuberculosis possess several unique and structurally diverse lipids, including the genus-specific mycolic acids. Although the function of a number of genes involved in fatty acid and mycolic acid biosynthesis is known, information relevant to the initial steps within these biosynthetic pathways is relatively sparse. Interestingly, the genomes of Corynebacterianeae possess a high number of accD genes, whose gene products resemble the beta-subunit of the acetyl-CoA carboxylase of Escherichia coli, providing the activated intermediate for fatty acid synthesis. We present here our studies on four putative accD genes found in C. glutamicum. Although growth of the accD4 mutant remained unchanged, growth of the accD1 mutant was strongly impaired and partially recovered by the addition of exogenous oleic acid. Overexpression of accD1 and accBC, encoding the carboxylase alpha-subunit, resulted in an 8-fold increase in malonyl-CoA formation from acetyl-CoA in cell lysates, providing evidence that accD1 encodes a carboxyltransferase involved in the biosynthesis of malonyl-CoA. Interestingly, fatty acid profiles remained unchanged in both our accD2 and accD3 mutants, but a complete loss of mycolic acids, either as organic extractable trehalose and glucose mycolates or as cell wall-bound mycolates, was observed. These two carboxyltransferases are also retained in all Corynebacterianeae, including Mycobacterium leprae, constituting two distinct groups of orthologs. Furthermore, carboxyl fixation assays, as well as a study of a Cg-pks deletion mutant, led us to conclude that accD2 and accD3 are key to mycolic acid biosynthesis, thus providing a carboxylated intermediate during condensation of the mero-chain and alpha-branch directed by the pks-encoded polyketide synthase. This study illustrates that the high number of accD paralogs have evolved to represent specific variations on the well known basic theme of providing carboxylated intermediates in lipid biosynthesis. (+info)

Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. (5/403)

In Corynebacterium glutamicum, the activity of aconitase is 2.5-4-fold higher on propionate, citrate, or acetate than on glucose. Here we show that this variation is caused by transcriptional regulation. In search for putative regulators, a gene (acnR) encoding a TetR-type transcriptional regulator was found to be encoded immediately downstream of the aconitase gene (acn) in C. glutamicum. Deletion of the acnR gene led to a 5-fold increased acn-mRNA level and a 5-fold increased aconitase activity, suggesting that AcnR functions as repressor of acn expression. DNA microarray analyses indicated that acn is the primary target gene of AcnR in the C. glutamicum genome. Purified AcnR was shown to be a homodimer, which binds to the acn promoter in the region from -11 to -28 relative to the transcription start. It thus presumably acts by interfering with the binding of RNA polymerase. The acn-acnR organization is conserved in all corynebacteria and mycobacteria with known genome sequence and a putative AcnR consensus binding motif (CAGNACnnncGTACTG) was identified in the corresponding acn upstream regions. Mutations within this motif inhibited AcnR binding. Because the activities of citrate synthase and isocitrate dehydrogenase were previously reported not to be increased during growth on acetate, our data indicate that aconitase is a major control point of tricarboxylic acid cycle activity in C. glutamicum, and they identify AcnR as the first transcriptional regulator of a tricarboxylic acid cycle gene in the Corynebacterianeae. (+info)

Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease-encoding genes in Corynebacterium glutamicum. (6/403)

The molecular identification of the Corynebacterium glutamicum urea uptake system is described. This ABC-type transporter is encoded by the urtABCDE operon, which is transcribed in response to nitrogen limitation. Expression of the urt genes is regulated by the global nitrogen regulator AmtR, and an amtR deletion strain showed constitutive expression of the urtABCDE genes. The AmtR repressor protein also controls transcription of the urease-encoding ureABCEFGD genes in C. glutamicum. The ure gene cluster forms an operon which is mainly transcribed in response to nitrogen starvation. To confirm the increased synthesis of urease subunits under nitrogen limitation, proteome analyses of cytoplasmic protein extracts from cells grown under nitrogen surplus and nitrogen limitation were carried out, and five of the seven urease subunits were identified. (+info)

Integration of E. coli aroG-pheA tandem genes into Corynebacterium glutamicum tyrA locus and its effect on L-phenylalanine biosynthesis. (7/403)

AIM: To study the effect of integration of tandem aroG-pheA genes into the tyrA locus of Corynebacterium glutamicum (C. glutamicum) on the production of L-phenylalanine. METHODS: By nitrosoguanidine mutagenesis, five p-fluorophenylalanine (FP)-resistant mutants of C.glutamicum FP were selected. The tyrA gene encoding prephenate dehydrogenase (PDH) of C.glutamicum was amplified by polymerase chain reaction (PCR) and cloned on the plasmid pPR. Kanamycin resistance gene (Km) and the P(BF) -aroG-pheA-T (GA) fragment of pGA were inserted into tyrA gene to form targeting vectors pTK and pTGAK, respectively. Then, they were transformed into C.glutamicum FP respectively by electroporation. Cultures were screened by a medium containing kanamycin and detected by PCR and phenotype analysis. The transformed strains were used for L-phenylalanine fermentation and enzyme assays. RESULTS: Engineering strains of C.glutamicum (Tyr(-)) were obtained. Compared with the original strain, the transformed strain C. glutamicum GAK was observed to have the highest elevation of L-phenylalanine production by a 1.71-fold, and 2.9-, 3.36-, and 3.0-fold in enzyme activities of chorismate mutase, prephenate dehydratase and 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase, respectively. CONCLUSION: Integration of tandem aroG-pheA genes into tyrA locus of C. glutamicum chromosome can disrupt tyrA gene and increase the yield of L-phenylalanine production. (+info)

Cometabolism of a nongrowth substrate: L-serine utilization by Corynebacterium glutamicum. (8/403)

Despite its key position in central metabolism, L-serine does not support the growth of Corynebacterium glutamicum. Nevertheless, during growth on glucose, L-serine is consumed at rates up to 19.4 +/- 4.0 nmol min(-1) (mg [dry weight])(-1), resulting in the complete consumption of 100 mM L-serine in the presence of 100 mM glucose and an increased growth yield of about 20%. Use of 13C-labeled L-serine and analysis of cellularly derived metabolites by nuclear magnetic resonance spectroscopy revealed that the carbon skeleton of L-serine is mainly converted to pyruvate-derived metabolites such as L-alanine. The sdaA gene was identified in the genome of C. glutamicum, and overexpression of sdaA resulted in (i) functional L-serine dehydratase (L-SerDH) activity, and therefore conversion of L-serine to pyruvate, and (ii) growth of the recombinant strain on L-serine as the single substrate. In contrast, deletion of sdaA decreased the L-serine cometabolism rate with glucose by 47% but still resulted in degradation of L-serine to pyruvate. Cystathionine beta-lyase was additionally found to convert L-serine to pyruvate, and the respective metC gene was induced 2.4-fold under high internal L-serine concentrations. Upon sdaA overexpression, the growth rate on glucose is reduced 36% from that of the wild type, illustrating that even with glucose as a single substrate, intracellular L-serine conversion to pyruvate might occur, although probably the weak affinity of L-SerDH (apparent Km, 11 mM) prevents substantial L-serine degradation. (+info)

In Corynebacterium glutamicum, it achieves this by antisense pairing with the mRNA of RepB, a replication initiation protein. ... "Control of rep gene expression in plasmid pGA1 from Corynebacterium glutamicum". J Bacteriol. 185 (8): 2402-2409. doi:10.1128/ ...

https://en.wikipedia.org/wiki/CtRNA

Hwang, B. J.; Yeom, H. J.; Kim, Y.; Lee, H. S. (2002). "Corynebacterium glutamicum utilizes both transsulfuration and direct ...

https://en.wikipedia.org/wiki/Transsulfuration_pathway

"Efflux permease CgAcr3-1 of Corynebacterium glutamicum is an arsenite-specific antiporter". The Journal of Biological Chemistry ... from Alkaliphilus metalliredigens and Corynebacterium glutamicum". The Journal of Biological Chemistry. 284 (30): 19887-95. doi ...

https://en.wikipedia.org/wiki/ARC3_family

Metabolic Engineering of the Valine Pathway in Corynebacterium Glutamicum - Analysis and Modelling. Germany: Forschungszentrum ...

https://en.wikipedia.org/wiki/Xerlin

The production of these amino acids is due to Corynebacterium glutamicum and fermentation. C.glutamicum was engineered to be ... L-Lysine was originally produced from diaminopimelic acid (DAP) by E.coli, but once the C.glutamicum was discovered for the ... L-Tryptophan is also produced through fermentation and by Corynebacterium and E.coli, though the production is not as large as ... "Corynebacterium species , Johns Hopkins ABX Guide". www.hopkinsguides.com. Retrieved 2019-11-11. Singhania, Reeta Rani; Patel, ...

https://en.wikipedia.org/wiki/Industrial_microbiology

Manufacturers, such as Ajinomoto, use selected strains of Corynebacterium glutamicum bacteria in a nutrient-rich medium. The ...

https://en.wikipedia.org/wiki/Glutamate_flavoring

It is found in a variety of bacteria, including Bacillus subtilis, Escherichia coli and Corynebacterium glutamicum. It is ...

https://en.wikipedia.org/wiki/LysC

"High level expression of Streptomyces mobaraensis transglutaminase in Corynebacterium glutamicum using a chimeric pro-region ...

https://en.wikipedia.org/wiki/Streptomyces_cinnamoneus

... and Corynebacterium glutamicum. Metabolic engineering for the production of high quantities of succinic acid should be ...

https://en.wikipedia.org/wiki/Basfia_succiniciproducens

An example is lysine production with homoserine- or threonine/methionine-requiring mutant of Corynebacterium glutamicum being ...

https://en.wikipedia.org/wiki/Fed-batch_culture

... of cyanophycin in a number of biotechnologically relevant bacteria such as Escherichia coli and Corynebacterium glutamicum. ...

https://en.wikipedia.org/wiki/Cyanophycin

"A systematic method to identify genomic islands and its applications in analyzing the genomes of Corynebacterium glutamicum and ...

https://en.wikipedia.org/wiki/Z_curve

... the difference in glucose repression of two β-glucoside phosphotransferase system gene clusters in Corynebacterium glutamicum R ...

https://en.wikipedia.org/wiki/Bglg-cis-reg_RNA

... conducting basic microbiology research on the soil bacteria Corynebacterium glutamicum, which is used in the production of ...

https://en.wikipedia.org/wiki/Nathan_H._Lents

... used to produce a number of fermented soy products Biotechnology in pharmaceutical manufacturing Corynebacterium glutamicum, ...

https://en.wikipedia.org/wiki/Bacillus_isolates

Identification of constraints to lysine production in Corynebacterium glutamicum and insertion of new genes to relieve these ...

https://en.wikipedia.org/wiki/Metabolic_engineering

Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. Corynebacterium glutamicum ATCC 14067 was ... Genome sequence for C. glutamicum from NCBI. Type strain of Corynebacterium glutamicum at BacDive - the Bacterial Diversity ... Corynebacterium glutamicum (previously known as Micrococcus glutamicus) is a Gram-positive, rod-shaped bacterium that is used ... 4 September 2003). "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l- ...

https://en.wikipedia.org/wiki/Corynebacterium_glutamicum

Date M, Itaya H, Matsui H, Kikuchi Y (January 2006). "Secretion of human epidermal growth factor by Corynebacterium glutamicum ... Corynebacterium[edit]. Non-pathogenic species of the gram-positive Corynebacterium are used for the commercial production of ... "The transcriptional regulatory repertoire of Corynebacterium glutamicum: reconstruction of the network controlling pathways ... The C. glutamicum species is widely used for producing glutamate and lysine,[14] components of human food, animal feed and ...

https://en.wikipedia.org/wiki/Protein_expression_(biotechnology)

... its regulation and biotechnological application in Corynebacterium glutamicum". Microbial Biotechnology. 7 (1): 5-25. doi: ... "Modification of histidine biosynthesis pathway genes and the impact on production of L-histidine in Corynebacterium glutamicum ...

https://en.wikipedia.org/wiki/L-Histidine

... its regulation and biotechnological application in Corynebacterium glutamicum". Microbial Biotechnology. 7 (1): 5-25. doi: ...

https://en.wikipedia.org/wiki/Amino_acid_synthesis

August 2012) "Bio-based production of chemicals, materials and fuels -Corynebacterium glutamicum as versatile cell factory". ... glutamicum of E. coli. Partykeer word geneties gemodifiseerde bakterië gebruik. Die bakterië wat gebruik word het almal ...

https://af.wikipedia.org/wiki/Triptofaan

Corynebacterium glutamicum (කොරයින\බැක්ටීරියm, ග්ලූටැමීkම්) යනු වාර්ෂික නිෂ්පාදනය ටොන් මිලියන දෙකක් පමණ වන ඇමයිනෝ අම්ල ...

https://si.wikipedia.org/wiki/කාර්මික_ක්ෂුද්‍ර_ජීව_විද්‍යාව

... with the organism Corynebacterium glutamicum (also known as Brevibacterium flavum) being the most widely used for production.[ ...

https://en.wikipedia.org/wiki/Glutamic_acid

Corynebacterium glutamicum ou Escherichia coli. Estas estirpes bacterianas teñen mutacións que impiden a recaptación de ...

https://gl.wikipedia.org/wiki/Triptófano

... with the organism Corynebacterium glutamicum (also known as Brevibacterium flavum) being the most widely used for production.[ ...

https://en.m.wikipedia.org/wiki/Glutamic_acid

... used to produce a number of fermented soy products Biotechnology in pharmaceutical manufacturing Corynebacterium glutamicum, ...

https://en.wikipedia.org/wiki/Streptomyces_isolates

https://en.wikipedia.org/wiki/Corynebacterium_glutamicum

Corynebacterium glutamicum (strain R). Corynebacterium glutamicum (Brevibacterium saccharolyticum). Corynebacterium glutamicum ... Corynebacterium glutamicum ATCC 14067. Corynebacterium crenatum MT. [Brevibacterium] flavum. Corynebacterium glutamicum (strain ... Corynebacterium glutamicum (Brevibacterium saccharolyticum). Corynebacterium glutamicum ATCC 14067. Corynebacterium crenatum MT ... Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025). Corynebacterium glutamicum ( ...

http://www.uniprot.org/uniprot/A0A072Z841

Isolation of plasmid DNA from Corynebacterium glutamicum using the QIAGEN® Plasmid Mini Kit - (EN). ... of different medium-copy-number plasmids carrying pHM1519 or pBL1 origins of replication from Corynebacterium glutamicum ATCC ...

https://www.qiagen.com/uz/resources/resourcedetail?id=1ad9b140-880d-44c3-ac9f-110cbaae599e&lang=en

Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... The Corynebacterium glutamicum genome: features and impacts on biotechnological processes. Appl. Microbiol. Biotechnol. 62:99- ...

https://aem.asm.org/content/71/6/3369?ijkey=b3f272b52f13b7c69728837f62a2157e6969b290&keytype2=tf_ipsecsha

Methionine uptake and excretion in Corynebacterium glutamicum. The characterization of methionine uptake in C. glutamicum ... Methioninaufnahme und -export in Corynebacterium glutamicum. Die Untersuchung der Methioninaufnahme in C. glutamicum führte zur ... Um den Methioninexport in C. glutamicum zu charakterisieren, wurde ein Beladungssystem mit L-Methionin-haltigen Dipeptiden ... glutamicum. The expression of this gene cluster encoding an ABC transporter is regulated by the repressor McbR. The second ...

https://kups.ub.uni-koeln.de/1529/

The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium ... Corynebacterium diphtheriae, Corynebacterium jeikeium, and mycobacterium [1-3]. C. glutamicum cells are frequently confronted ... CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum Meiru Si Meiru Si ... Corynebacterium glutamicum is a high G+C-content aerobic Gram-positive bacterium. It is not only a workhorse in biotechnology ...

https://portlandpress.com/biochemj/article/475/24/3979/49840/CosR-is-an-oxidative-stress-sensing-a-MarR-type

... glutamicum systems biology and biotechnological applications including: proteomics; flux analysis technology; metabolic ... Corynebacterium glutamicum is most widely known for its role in the industrial production of L-glutamate and L-lysine and as a ... Corynebacterium glutamicum is often used as a model organism because of its ability to produce various useful substances. For ... Corynebacterium glutamicum is well known for the multi-million-ton-scale production of L-glutamate and L-lysine in ...

https://www.caister.com/cory2

As the first complete compilation of major findings, Handbook of Corynebacterium glutamicum is a comprehensive source of ... Corynebacterium glutamicum is currently used to produce 2 million tons of amino acids per year for a rapidly expanding market. ... Just as C. glutamicum has proven its profitability in industry and research, this book will demonstrate its value to the ... glutamicum research from its discovery in 1957 through the most recent studies at the genomic and systemic level, and provides ...

https://routledgehandbooks.com/doi/10.1201/9781420039696.ch5

Efflux permease CgAcr3-1 of Corynebacterium glutamicum is an arsenite-specific antiporter.. Title. Efflux permease CgAcr3-1 of ... We analyzed the function of three Acr3 proteins from Corynebacterium glutamicum, CgAcr3-1, CgAcr3-2, and CgAcr3-3. CgAcr3-1 ... Corynebacterium glutamicum, Hydrogen-Ion Concentration, Hydrophobic and Hydrophilic Interactions, Membrane Potentials, Membrane ... CgAcr3-1 was also the most active when everted membranes vesicles from Escherichia coli or C. glutamicum mutants were assayed ...

http://sbrc.vub.ac.be/node/162

Page contains details about Corynebacterium glutamicum catalase-gold nanoparticles nanostructured material . It has composition ... 4 4 DBCO-modified oligo 2-functionalized Corynebacterium glutamicum catalase nanoparticles 1 thiol-modified oligo 1- ... DBCO-modified oligo 2-functionalized Corynebacterium glutamicum catalase nanoparticles DBCO-modified oligo 2-functionalized Cg ...

http://nano.nature.com/nano/GR-M7944060

C4 dicarboxylic acid transporters of Corynebacterium glutamicum.. *Javelle, Arnaud (Principal Investigator). *Birke, Anna (Post ...

https://pureportal.strath.ac.uk/en/projects/c4-dicarboxylic-acid-transporters-of-corynebacterium-glutamicum

Production of lysine by Corynebacterium glutamicum (PTCC 1532) from different agricultural by-products (molasses and pulpy ... Fermentative Production of Lysine by Corynebacterium glutamicum from Different Carbon Sources. Article 8, Volume 25.26, Issue ... Production of lysine by Corynebacterium glutamicum (PTCC 1532) from different agricultural by-products (molasses and pulpy ... MOOSAVI-NASAB, M., ANSARI, S., MONTAZER, Z. Fermentative Production of Lysine by Corynebacterium glutamicum from Different ...

http://iar.shirazu.ac.ir/article_189.html

... Download vts_7733_11123. ... Corynebacterium glutamicum [GND]. Isoleucin [GND]. Biotechnology [LCSH]. Branched chain aminoacids [LCSH]. Metabolism [LCSH] ... The current work deals with the biotechnological synthesis of alpha-ketomethylvalerate (KMV) with Corynebacterium glutamicum. ... Analysis of the biotechnological production of 2-ketomethylvalerate with Corynebacterium glutamicum. Open Access Repositorium ...

https://oparu.uni-ulm.de/xmlui/handle/123456789/1952

... from Corynebacterium glutamicum R. Plus protein sequence and external database links. ... Domain assignment for gi,145295828,ref,YP_001138649.1, from Corynebacterium glutamicum R. Domain architecture ... hypothetical protein cgR_1753 [Corynebacterium glutamicum R]. Sequence. ...

https://supfam.mrc-lmb.cam.ac.uk/SUPERFAMILY/cgi-bin/gene.cgi?genome=4e&seqid=gi%7C145295828%7Cref%7CYP_001138649.1%7C

Biosorptive Decolorization of Reactive Orange 16 Using the Waste Biomass of Corynebacterium glutamicum ...

https://www.infona.pl/resource/bwmeta1.element.acs-doi-10_1021_ie049559o

... glutamicum. Understanding its cell physiology will help manufacturers to increase their product range and productivity through ... Pathways at Work: Metabolic Flux Analysis of the Industrial Cell Factory Corynebacterium glutamicum ... genome engineering tools and comprehensive analysis techniques for Corynebacterium glutamicum. Aside from modern omics-based ... Newly added topics include cell envelope structures and aromatic compound metabolism in C. glutamicum. These chapters will be ...

https://www.springer.com/jp/book/9783030392666

Engineering Corynebacterium glutamicum for isobutanol production. Appl Microbiol Biotechnol. 2010;87:1045-55.CrossRefPubMed ... Metabolic engineering of Corynebacterium glutamicum for l-arginine production. Nat Commun. 2014;5:4618.CrossRefPubMedGoogle ... Corynebacterium glutamicum, having been widely used in industrial production of amino acids such as L-glutamate and l-lysine [ ... Bio-based production of organic acids with Corynebacterium glutamicum. Microb Biotechnol. 2013;6:87-102.CrossRefPubMedGoogle ...

https://link.springer.com/article/10.1186%2Fs12934-018-0990-z

PcaO Positively Regulates pcaHG of the β-Ketoadipate Pathway in Corynebacterium glutamicum Ke-Xin Zhao, Yan Huang, Xi Chen, Nan ... Polynucleotide Phosphorylase, RNase E/G, and YbeY Are Involved in the Maturation of 4.5S RNA in Corynebacterium glutamicum ... Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum Jung-Won Youn, Elena Jolkver, Reinhard ... Subcellular Localization and Characterization of the ParAB System from Corynebacterium glutamicum Catriona Donovan, Astrid ...

https://jb.asm.org/keyword/corynebacterium-glutamicum

Corynebacterium glutamicum Description and significance. C. glutamicum is a small, non-moving Gram-positive soil bacterium. ... "Corynebacterium glutamicum as a model bacterium for the bioremediation of arsenic". International Microbiology. 2006. p. 207- ... "Reduced Folate Supply as a Key to Enhanced L-Serine Production by Corynebacterium glutamicum." Applied and Environmental ... Another possible use for C. glutamicum is in bioremediation, such as for arsenic. C. glutamicum contains two operons in its ...

https://microbewiki.kenyon.edu/index.php/Corynebacterium_glutamicum

... glutamicum), an important industrial workhorse, is capable of efficiently producing a variety of value-added chemicals and ... Corynebacterium glutamicum (C. glutamicum), an important industrial workhorse, is capable of efficiently producing a variety of ... Corynebacterium glutamicum Renewable sources Synthetic biology Metabolic engineering This is a preview of subscription content ... Yang J, Zhu Y, Men Y, Sun S, Zeng Y, Zhang Y, Sun Y, Ma Y (2016) Pathway construction in Corynebacterium glutamicum and strain ...

https://link.springer.com/article/10.1007%2Fs00253-018-9358-x

Corynebacterium glutamicum ATCC 13032. Mutation(s): 0 Gene Names: dapF, Cgl1943, cg2129. EC: 5.1.1.7. ... Find proteins for Q8NP73 (Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)) ... Here, we report the crystal structure of DapF from Corynebacterium glutamicum (CgDapF). Structures of CgDapF obtained under ... Structural basis for redox sensitivity in Corynebacterium glutamicum diaminopimelate epimerase: an enzyme involved in l-lysine ...

https://www.rcsb.org/structure/5h2y

Corynebacterium glutamicum is the prominent host for the fermentative production of amino acids with an average annual ... Due to its robustness and well established large-scale fermentation, C. glutamicum has been engineered for the production of a ... In this study, systematic metabolic engineering enabled construction of a patchoulol producing C. glutamicum strain by applying ... Corynebacterium glutamicum; sesquiterpene; metabolic engineering; algae; Escherichia coli patchoulol; Corynebacterium ...

https://www.mdpi.com/2073-4425/9/4/219

provide insights into the roles of DNA partitioning protein ParB and SMC condensin complexes in Corynebacterium glutamicum. ... the role of DNA-partitioning protein ParB and SMC condensin complexes in the actinobacterium Corynebacterium glutamicum. ... 36 to unveil the global organization of the diploid Corynebacterium glutamicum genome. C. glutamicum is a polar growing ... 3: Functional characterization of two SMC-like complexes in C. glutamicum.. a Sections of the C. glutamicum genome map ...

https://www.nature.com/articles/s41467-020-15238-4?error=cookies_not_supported&code=f5850840-8471-487e-9ff3-d741ffa3bba2

... developed a model system for the biodegradation of phenol-contaminated wastewater by the bacterium Corynebacterium glutamicum. ... A. Nardi, R. Avrahami, E. Zussman, J. Rokem and C. Greenblatt, "Phenol Biodegradation by Corynebacterium glutamicum ... Is Involved in Intercellular Communication of Corynebacterium glutamicum," Archives of Microbiology, Vol. 182, No. 4, 2004, pp ... developed a model system for the biodegradation of phenol-contaminated wastewater by the bacterium Corynebacterium glutamicum. ...

https://www.scirp.org/journal/PaperInformation.aspx?PaperID=17442

... BMC Mol Biol. 2009 Jul 23;10:73. doi: 10.1186/ ... Background: The TetR family member AmtR is the central regulator of nitrogen starvation response in Corynebacterium glutamicum ...

https://pubmed.ncbi.nlm.nih.gov/19627583/?dopt=Abstract

Corynebacterium glutamicum (Brevibacterium saccharolyticum)Imported. Automatic assertion inferred from database entriesi ... tr,Q46061,Q46061_CORGT Beta C-S lyase OS=Corynebacterium glutamicum OX=1718 GN=aecD PE=4 SV=2 ...

https://www.uniprot.org/uniprot/Q46061.html

Corynebacterium glutamicum ATCC ® 13032™ Designation: 534 TypeStrain=True Application: Produces glutamic acid L-glutamate, L- ... Nucleotide (GenBank) : Y13221 Corynebacterium glutamicum glnA gene. Nucleotide (GenBank) : AJ238250 Corynebacterium glutamicum ... Nucleotide (GenBank) : Y17592 Corynebacterium glutamicum accDA gene. Nucleotide (GenBank) : AJ012293 Corynebacterium glutamicum ... Corynebacterium glutamicum, gdh gen for glutamate dehydrogenase. Nucleotide (GenBank) : U31281 Corynebacterium glutamicum ...

https://www.atcc.org/Products/All/13032?geo_country=us

... methanethiol and dimethyldisulfide were investigated as sulfur source for methionine synthesis in Corynebacterium glutamicum. ... In vitro studies with the C. glutamicum wild type revealed a relatively low activity of MetY for methanethiol (63 mU/mg) and ... Corynebacterium glutamicum/genetics. *Corynebacterium glutamicum/growth & development. *Corynebacterium glutamicum/metabolism* ...

https://www.ncbi.nlm.nih.gov/pubmed/20798582?dopt=Abstract

We previously described a role of RodA from Corynebacterium glutamicum in apical cell growth and morphogenesis. Deletion of ... We previously described a role of RodA from Corynebacterium glutamicum in apical cell growth and morphogenesis. Deletion of ... Efficient electrotransformation of Corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum ... Eikmanns, B. J., Kleinertz, E., Liebl, W., and Sahm, H. (1991). A family of Corynebacterium glutamicum/Escherichia coli shuttle ...

https://www.frontiersin.org/articles/10.3389/fmicb.2014.00738/full

Corynebacterium glutamicum. Mutation(s): 0 Gene Names: ispB, Cgl0473, cg0559. EC: 2.5.1.30. ... Find proteins for Q8NT37 (Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)) ... Crystal Structure of Geranylgeranyl Pyrophosphate Synthase from Corynebacterium Glutamicum. Patskovsky, Y., Ho, M., Toro, R., ... Crystal structure of geranylgeranyl pyrophosphate synthase from corynebacterium glutamicum atcc 13032. *DOI: 10.2210/pdb3LMD/ ...

https://www.rcsb.org/structure/3lmd

New Trends in Corynebacterium glutamicum: Beyond the Amino Acids. Carlos Barreiro (Editor). Instituto de Biotecnología de León ... Use of Corynebacterium glutamicum for Heavy Metals Bioremediation: Arsenic as Prototype. (Almudena F. Villadangos, José A. Gil ... Home / Shop / Books / Science and Technology / Life Sciences / New Trends in Corynebacterium glutamicum: Beyond the Amino Acids ... L-Valine production in Corynebacterium glutamicum. (Kazumi Hiraga, Crispinus A. Omumasaba, Masayuki Inui, Research Institute of ...

https://novapublishers.com/shop/new-trends-in-corynebacterium-glutamicum-beyond-the-amino-acids/

Advancing Dual-Phase Production Strategies with Corynebacterium Glutamicum through Systems Biology. *Conference: Metabolic ... To address this question, we systematically investigate the adaptation of the industrially relevant Corynebacterium glutamicum ... Conclusion. The established bioprocess is an elegant approach for the systematic understanding of C. glutamicums adaptation to ... Methods. A "triple-phase" batch bioprocess with C. glutamicum was established that depicts the three successive phases ( ...

https://www.aiche.org/sbe/conferences/metabolic-engineering-conference/2016/proceeding/paper/advancing-dual-phase-production-strategies-corynebacterium-glutamicum-through-systems-biology

The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium ... Corynebacterium glutamicum is a high-G+C content gram-positive soil bacterium which was isolated in 1957 as an l-glutamate- ... The DeoR-Type Regulator SugR Represses Expression of ptsG in Corynebacterium glutamicum Verena Engels, Volker F. Wendisch ... Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. J. Biol. Chem. 280:585- ...

https://jb.asm.org/content/189/8/2955?ijkey=15624fb43155158011fe764c3516ac5c8f1fac18&keytype2=tf_ipsecsha

The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived ... The complete genomic sequence of Corynebacterium glutamicum ATCC 13032, well-known in industry for the production of amino ... The C. glutamicum genome was found to consist of a single circular chromosome comprising 3282708 base pairs. Several DNA ... These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity ...

https://www.ncbi.nlm.nih.gov/pubmed/12948626?dopt=Abstract

... glutamicum under the given conditions. However, glucose consumption for cell preparation decreases process yield (defined as ... Aerobically cultivated cells of Corynebacterium glutamicum produce mixed organic acids, including succinic acid (SA), from ... Keywords: Corynebacterium glutamicum; oxygen deprivation; succinic acid; cell recycle reaction Corynebacterium glutamicum; ... Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant Corynebacterium glutamicum. Toru Jojima 1 ...

http://www.mdpi.com/2311-5637/2/1/5

Amino acid exporters in Corynebacterium glutamicum, p 335-349. In Yukawa H, Inui M (ed), Corynebacterium glutamicum. Springer, ... A Novel Corynebacterium glutamicum l-Glutamate Exporter. Yu Wang, Guoqiang Cao, Deyu Xu, Liwen Fan, Xinyang Wu, Xiaomeng Ni, ... A Novel Corynebacterium glutamicum l-Glutamate Exporter. Yu Wang, Guoqiang Cao, Deyu Xu, Liwen Fan, Xinyang Wu, Xiaomeng Ni, ... A Novel Corynebacterium glutamicum l-Glutamate Exporter. Yu Wang, Guoqiang Cao, Deyu Xu, Liwen Fan, Xinyang Wu, Xiaomeng Ni, ...

https://aem.asm.org/content/84/6/e02691-17

Key words: Corynebacterium glutamicum, L-leucine, metabolic engineering, fermentation, industrial production. ... Key words: Corynebacterium glutamicum, L-leucine, metabolic engineering, fermentation, industrial production. ... A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase ( ... A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase ( ...

https://academicjournals.org/journal/AJB/article-abstract/12183F164057

Key words: Corynebacterium glutamicum, L-leucine, metabolic engineering, fermentation, industrial production. ... A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase ( ... IPMS) that carries three amino acid exchanges (R529H, G532D and L535V) from the mutant strain C. glutamicum ML1-9 which was ... This work aimed to develop an efficient L-leucine industrial production strain of Corynebacterium glutamicum by using metabolic ...

https://academicjournals.org/journal/AJB/article-references/12183F164057

Corynebacterium glutamicum, NanR, Glucosamin, Lysin, N-Acetylglucosamin, Neuraminsäure, Regulation. German. Corynebacterium ... Aminozucker, wie Glucosamin oder N-Acetylglucosamin, stellen für biotechno-logische Prozesse mit Corynebacterium glutamicum ... Als Bestandteil von Chitin sind sie weit verbreitet, können jedoch von C. glutamicum nur sehr schlecht oder, wie im Fall von N- ... Over-expression of nagB in C. glutamicum tripled growth rates on glucosamine. Furthermore, the regulator of the nag-genes was ...

https://kups.ub.uni-koeln.de/5498/

Gram-pozitivní, aerobní bakterie Corynebacterium glutamicum je významným producentem aminokyselin. V genomu C. glutamicum se ... Účast alternativních sigma faktorů RNA polymerasy při regulaci exprese genů Corynebacterium glutamicum. *Detail práce ... Corynebacterium glutamicum, sigma faktor, anti-sigma faktor, promotor, tepelný šok, in-vitro ... The role of alternative sigma factors of RNA polymerase in regulation of gene expression in Corynebacterium glutamicum ...

https://is.cuni.cz/webapps/zzp/detail/200768/?lang=cs

Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. (wikipedia.org)
Corynebacterium glutamicum ATCC 14067 was previously known as Brevibacterium flavum. (wikipedia.org)
The procedure has been used successfully for isolation of different medium-copy-number plasmids carrying pHM1519 or pBL1 origins of replication from Corynebacterium glutamicum ATCC 13032. (qiagen.com)
The Corynebacterium glutamicum ATCC 13032 prophage CGP3 encodes an actin-like protein, AlpC that was shown to be involved in viral DNA transport and efficient viral DNA replication. (uni-muenchen.de)

Genome sequence for C. glutamicum from NCBI. (wikipedia.org)
To construct an improved C. glutamicum genome, we developed a precise genome excision method based on the Cre/ loxP recombination system and successfully deleted 11 distinct genomic regions identified by comparative analysis of C. glutamicum genomes. (asm.org)
By using the genome information of C. glutamicum , we recently found many strain-specific regions existing as "islands" in the common backbone ( 24 ). (asm.org)
These strain-specific islands (SSIs) were possibly shaped on the genome of the ancestral common strain of two C. glutamicum strains by the integration and deletion of many genes. (asm.org)
Although the genome of Corynebacterium glutamicum encodes a large number of the putative MarR-type transcriptional regulators, their physiological and biochemical functions have so far been limited to only two proteins, regulator of oxidative stress response RosR and quinone oxidoreductase regulator QosR. (portlandpress.com)
The procedure was applied on data set detached from growth phenotyping of library of genome reduced C. glutamicum strains and results agree with previously reported results where manual effort was needed to pre-process the data. (biomedcentral.com)

These were obtained with Corynebacterium glutamicum showing single exponential growth phase and Escherichia coli exhibiting diauxic growth with exponential phase followed by retarded growth. (biomedcentral.com)

It is not only a workhorse in biotechnology for glutamate and lysine amino acid production, which are used as nutritive additives in food and feed, but also a model organism of systems biology for the investigation of its pathogenic relatives, Corynebacterium diphtheriae , Corynebacterium jeikeium , and mycobacterium [ 1 - 3 ]. (portlandpress.com)
Corynebacterium glutamicum is most widely known for its role in the industrial production of L-glutamate and L-lysine and as a platform organism for the production of a variety of fine chemicals, biofuels and polymers. (caister.com)
this in turn makes C. glutamicum the model organism of choice in white biotechnology. (caister.com)
With a current annual production of more than 2.9 million tons of L-glutamate and more than 1.9 million tons of L-lysine (Ajinomoto, 2013a, b) C. glutamicum already represents a key organism for industrial biotechnology. (caister.com)
Based on these studies, it becomes increasingly obvious that C. glutamicum has an enormous potential for the biotechnological synthesis of many compounds and materials, making C. glutamicum a versatile platform organism and biotechnology workhorse. (caister.com)

We chose to delete each of 11 SSIs that are larger than 10 kb among thousands of C. glutamicum R strain-specific islands. (asm.org)
As a basis for the construction of a KMV production strain the L-lysine producer C. glutamicum DM1729 was employed. (uni-ulm.de)
The resulting strain C. glutamicum DM1729 Delta-ilvE (pEK hom FBR thrB thrC) (pMM36ptac ilvA) is the first described strain for the intentional production of KMV from glucose. (uni-ulm.de)

Technologies for quantification, membrane enrichment and MS analysis tested in C. glutamicum might lead to higher coverage for membrane proteins or improve the accuracy and reliability of quantification based on isotope labeling. (caister.com)

The characterization of methionine uptake in C. glutamicum revealed two uptake systems. (uni-koeln.de)

Corynebacterium glutamicum: Biology and Biotechnology. (wikipedia.org)
Packed with practical information and state-of-the-art science this concise volume is an essential handbook for everyone working with Corynebacterium and related organisms in academia, biotechnology companies, and the pharmaceutical industry and is a recommended volume for all microbiology libraries. (caister.com)
Industrial production of amino acid, a key sector of white biotechnology, is the classical field of application of Corynebacterium glutamicum . (caister.com)

Here, we report that the ncgl2617 gene ( cosR ) of C. glutamicum encoding an MarR-type transcriptional regulator plays an important role in oxidative stress resistance. (portlandpress.com)
Together, the results reveal that CosR is a redox-sensitive regulator that senses peroxide stress to mediate oxidative stress resistance in C. glutamicum . (portlandpress.com)

Corynebacterium glutamicum (previously known as Micrococcus glutamicus) is a Gram-positive, rod-shaped bacterium that is used industrially for large-scale production of amino acids. (wikipedia.org)
The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. (asm.org)
C. glutamicum cells are frequently confronted with excessive reactive oxygen species (ROS) production, triggering sudden changes of fermentative conditions in temperature, pH, osmotic pressure, or toxic compounds [ 4 - 5 ]. (portlandpress.com)
2007). Furthermore, within the last decade, C. glutamicum was applied in various production processes for fine chemicals, fuels and polymers. (caister.com)
Proteomics is an important technique to study the biology of Corynebacterium glutamicum with focus on processes related to the biotechnological production of amino acids and other chemicals. (caister.com)

Corynebacterium glutamicum is a high G+C - content aerobic Gram-positive bacterium. (portlandpress.com)

Since most examples of regulation of gene expression in C . glutamicum describe transcriptional control, the main focus of this chapter is this level of regulation. (routledgehandbooks.com)

While originally identified in a screen for organisms secreting L-glutamate, mutants of C. glutamicum have also been identified that produce various other amino acids. (wikipedia.org)
The methods developed in C. glutamicum have been and can be applied to other organisms. (caister.com)
Corynebacterium glutamicum , like other organisms, must react to changes in its environment to ensure that nutrition sources will be utilized economically and that the cell will adapt to various conditions. (routledgehandbooks.com)

The current work deals with the biotechnological synthesis of alpha-ketomethylvalerate (KMV) with Corynebacterium glutamicum. (uni-ulm.de)

Thus, the ability of C. glutamicum to combat ROS challenges and maintain the cellular redox balance is crucial for cell survival and improves fermentation efficiency. (portlandpress.com)

Um den Methioninexport in C. glutamicum zu charakterisieren, wurde ein Beladungssystem mit L-Methionin-haltigen Dipeptiden etabliert. (uni-koeln.de)

This book provides a comprehensive overview of current knowledge and research on C. glutamicum systems biology and biotechnological applications. (caister.com)
In summary, proteomics data are important for systems biology approaches in C. glutamicum . (caister.com)

Datenbank-Analysen mit dem E. coli Methioninaufnahmesystem MetD als Vorlage führten zur Identifizierung der Gene metI, metN und metQ in C. glutamicum. (uni-koeln.de)
Data base searches with the E. coli methionine uptake system MetD as query resulted in the identification of the genes metI, metN and metQ in C. glutamicum. (uni-koeln.de)

Due to its industrial importance, several clones of C. glutamicum have been sequenced by both industry and academic groups. (wikipedia.org)

X96580 C.glutamicum panB, panC & xylB genes. (atcc.org)
Data base searches with the E. coli methionine uptake system MetD as query resulted in the identification of the genes metI, metN and metQ in C. glutamicum. (uni-koeln.de)
The sigA gene encodes the essential primary sigma factor of C. glutamicum and is responsible for promoter recognition of house-keeping genes. (biomedcentral.com)
Effect of carbon source availability and growth phase on expression of Corynebacterium glutamicum genes involved in the tricarboxylic acid cycle and glyoxylate bypass. (semanticscholar.org)
Regulation of the expression of phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) genes in Corynebacterium glutamicum R. (semanticscholar.org)
The transcriptional regulator SsuR activates expression of the Corynebacterium glutamicum sulphonate utilization genes in the absence of sulphate. (semanticscholar.org)
Due to the economical coding nature of vio operon, the compressed RBS of vio genes were replaced with complete strong C. glutamicum ones. (biomedcentral.com)
When the genes crtE, crtB and crtI for conversion of geranylgeranyl pyrophosphate to lycopene were overexpressed in C. glutamicum DeltacrtEb intensely red-pigmented cells and an 80 fold increased lycopene content of 2.4 +/- 0.3 mg/g CDW were obtained. (uni-bielefeld.de)
Conclusion C. glutamicum possesses a certain degree of redundancy in the biosynthesis of the C50 carotenoid decaprenoxanthin as it possesses two functional phytoene synthase genes. (uni-bielefeld.de)
Enzymes and genes of the isopropylmalate pathway leading to leucine in Corynebacterium glutamicum were studied, and assays were performed to unravel their connection to lysine oversynthesis. (mysciencework.com)
In Corynebacterium glutamicum R, the genes ptsH and ptsI encode general components of the PTS, and genes ptsF, ptsS and ptsG each encode fructose-, sucrose- and glucose-specific components of the PTS, respectively. (microbiologyresearch.org)
Control of the lysine biosynthetic sequence in Corynebacterium glutamicum as analyzed by overexpression of the individual corresponding genes. (microbiologyresearch.org)
Both genes placed under the control of the C. glutamicum ldhA promoter were expressed at high levels in C. glutamicum, resulting, under oxygen-deprivation conditions, in a significant yield ofethanol from glucose in a process characterized by the absence of cellular growth. (mendeley.com)
Initial experiments showed that sole overexpression of genes coding for the native malonyl-CoA-forming acetyl-CoA carboxylase is not sufficient for increasing polyphenol production in C. glutamicum. (unl.pt)
In this work, we showed that Corynebacterium glutamicum endowed with the Lactococcus lactis genes encoding α-acetolactate synthase and decarboxylase activities produced meso-2,3-BD as the major end product, meaning that (R)-acetoin is a substrate for endogenous 2,3-butanediol dehydrogenase (BDH) activity. (unl.pt)
Heterologous expression of the genes for both uptake and catabolism of D-galacturonic acid and D-glucuronic acid was required to enable growth of C. glutamicum with either aldohexuronic acid as the sole carbon source. (springeropen.com)

The Glycosylated Cell Surface Protein Rpf2, Containing a Resuscitation-Promoting Fac- tor Motif, Is Involved in Intercellular Communication of Corynebacterium glutamicum," Archives of Microbiology, Vol. 182, No. 4, 2004, pp. 299-312. (scirp.org)
SOS-induced spontaneous prophage induction in Corynebacterium glutamicum", Presented at the EMBL Heidelberg "New Approaches and Concepts in Microbiology", Heidelberg, Germany, 2013. (uni-bielefeld.de)

Corynebacterium glutamicum (previously known as Micrococcus glutamicus) is a Gram-positive, rod-shaped bacterium that is used industrially for large-scale production of amino acids. (wikipedia.org)
C. glutamicum is a small, non-moving Gram-positive soil bacterium. (kenyon.edu)
C. glutamicum is a non-pathogenic bacterium, although a related species, C. diphtheriae is pathogenic and causes diphtheria in humans through a strong exotoxin it produces. (kenyon.edu)
We developed a model system for the biodegradation of phenol-contaminated wastewater by the bacterium Corynebacterium glutamicum . (scirp.org)
Corynebacterium glutamicum is a high-G+C content gram-positive soil bacterium which was isolated in 1957 as an l -glutamate-excreting bacterium ( 1 , 32 ). (asm.org)
These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil. (nih.gov)
Amino acid- producing gram-positive bacterium Corynebacterium glutamicum codes for seven sigma factors: the primary sigma factor SigA, the primary-like sigma factor SigB and five ECF stress- responsive sigma factors (SigC, SigD, SigE, SigH and SigM). (cuni.cz)
Corynebacterium glutamicum is a high G+C - content aerobic Gram-positive bacterium. (portlandpress.com)
The gram positive soil bacterium Corynebacterium glutamicum thereby serves as major production organism. (cuvillier.de)
Corynebacterium glutamicum is a gram-positive soil bacterium widely used for the industrial production of amino acids. (biomedcentral.com)
C. glutamicum is a Gram-positive biotin-auxotrophic bacterium that was originally isolated as an L-glutamate producer from soil samples [ 8 ]. (biomedcentral.com)
Growth experiments revealed that the non-pathogenic soil bacterium C. glutamicum is able to use hemin or hemoglobin as sole iron source. (omicsdi.org)
The molecular cloning and sequence of the odhA gene encoding 2-oxoglutarate dehydrogenase (E1o subunit of the 2-oxoglutarate dehydrogenase complex) of Corynebacterium glutamicum, a coryneform, Gram-positive bacterium widely used in the industrial production of amino acids, are reported. (readabstracts.com)

Although the genome of Corynebacterium glutamicum encodes a large number of the putative MarR-type transcriptional regulators, their physiological and biochemical functions have so far been limited to only two proteins, regulator of oxidative stress response RosR and quinone oxidoreductase regulator QosR. (portlandpress.com)
However, the genome of C. glutamicum also encodes a second carotenoid gene cluster comprising crtB2I2-1/2 shown to be co-transcribed, as well. (uni-bielefeld.de)
The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (σ A ), the primary-like σ B and five alternative sigma factors (σ C , σ D , σ E , σ H and σ M ). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. (springeropen.com)
The C. glutamicum genome encodes seven sigma subunits of RNAP: the primary sigma factor σ A , the alternative primary-like σ B and five other alternative σ factors with extracytoplasmic functions (ECF) (σ C , σ D , σ E , σ H and σ M ) (for a review, see Pátek and Nešvera 2011 ). (springeropen.com)
The pck (cg3169) gene of Corynebacterium glutamicum encodes a phosphoenolpyruvate carboxykinase (PEPCK). (elsevier.com)
Neither aldohexuronic acid supported growth of C. glutamicum as sole or combined carbon source, although its genome encodes a putative uronate isomerase sharing 28% identical amino acids with UxaC from Escherichia coli . (springeropen.com)

C. glutamicum breaks down carbohydrates through the process of fermentation. (kenyon.edu)
Anusree M, Wendisch VF, Nampoothiri KM (2016) Co-expression of endoglucanase and beta-glucosidase in Corynebacterium glutamicum DM1729 towards direct lysine fermentation from cellulose. (springer.com)
Due to its robustness and well established large-scale fermentation, C. glutamicum has been engineered for the production of a number of value-added compounds including terpenoids. (mdpi.com)
Thus, the ability of C. glutamicum to combat ROS challenges and maintain the cellular redox balance is crucial for cell survival and improves fermentation efficiency. (portlandpress.com)
Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium 's GRAS status and advantages in tryptophan fermentation. (biomedcentral.com)
With metabolic engineering and fermentation optimization practice, C. glutamicum 21850 (pEC-C-vio1) was able to produce violacein with both titer and productivity at the highest level ever reported. (biomedcentral.com)
Due to advantages of mature C. glutamicum fermentation industry, this work has built basis for commercial production of violacein. (biomedcentral.com)
(EN) Disclosed is a L-isoleucine-producing Corynebacterium glutamicum fermentation medium, comprising a basal medium and a growth factor, wherein the growth factor consists of choline, betaine and vitamin B6, and the contents of each ingredient in the fermentation medium are: 0.2-1 g/L choline, 0.25-0.5 mg/L betaine, and 0.05-0.3 mg/L vitamin B6. (wipo.int)
Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on l‐threonine produced by fermentation with Corynebacterium glutamicum ■■■■■ when used as a nutritional additive in feed and water for drinking for all animal species and categories. (europa.eu)
This work addresses this problem, by studying the effect of some fermentation gradients (pH, glucose and oxygen) that occur at the larger scale in a bench‐scale two‐compartment reactor (Plug flow reactor (PFR) + Stirred tank reactor (STR)) using the cadaverine‐producing recombinant Corynebacterium glutamicum DM1945 Δact3 Ptuf‐ldcC_OPT. (lboro.ac.uk)
This study demonstrated that C. glutamicum DM1945 Δact3 Ptuf‐ldcC_OPT physiological response was a function of the magnitude of fermentation gradients simulated. (lboro.ac.uk)
Methods: The study was dealing with immobilization of C. glutamicum and mixed culture of C. glutamicum and Pseudomonas reptilivora P. reptilivora for L-glutamic acid production using submerged fermentation. (duhnnae.com)

Biosynthetic pathway of anthocyanins (labeled in pink) in plants and the introduced pathway for cyanidin 3- O -glucoside production from catechin in C. glutamicum . (springer.com)
The introduced pathway consisting of ANS and 3GT is highlighted in red, and the biosynthetic pathway of UDP-glucose in C. glutamicum is marked in green. (springer.com)
The central metabolic pathway of Corynebacterium glutamicum was engineered to produce ethanol. (mendeley.com)

Newly added topics include cell envelope structures and aromatic compound metabolism in C. glutamicum. (springer.com)
Due to the variance in the availability of nutrients and carbon sources, C. glutamicum has 127 proteins associated with a regulatory function in transcription, which in turn control metabolism. (kenyon.edu)
Through its metabolism, C. glutamicum synthesizes such products as serine, glutamate, and lysine (amino acids), all of which are used in many different ways, such as in pharmacies. (kenyon.edu)
The substrate spectrum of C. glutamicum has been further extended by detailed knowledge of carbon core metabolism and well-established genetic tools and engineering strategies. (springer.com)
Acetate metabolism and its regulation in Corynebacterium glutamicum. (academicjournals.org)
Previously, the gene cg0156 was shown to belong to the regulon of McbR, a global transcriptional repressor of sulphur metabolism in C. glutamicum. (sigmaaldrich.com)
Therefore, it is an interesting candidate for study to further the understanding of the regulation of sulphur metabolism in C. glutamicum. (sigmaaldrich.com)
Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR. (semanticscholar.org)
Complete sucrose metabolism requires fructose phosphotransferase activity in Corynebacterium glutamicum to ensure phosphorylation of liberated fructose. (microbiologyresearch.org)
Complementation experiments showed that a functional PTS phosphorelay is required for optimal growth of C. glutamicum on maltose, implying its involvement in the control of maltose metabolism and/or uptake. (microbiologyresearch.org)
This monograph illustrates how the data gleaned from whole genome sequencing permits the rational engineering of the total mobile metabolism and the way platforms biology allows the additional optimization of C. glutamicum as a biocatalyst. (globalinvests.com)
LldR (CGL2915) from Corynebacterium glutamicum is a transcription factor belonging to the GntR family, which is typically involved in the regulation of oxidized substrates associated with amino acid metabolism. (elsevier.com)
Marienhagen, Jan. / Modulation of the central carbon metabolism of Corynebacterium glutamicum improves malonyl-CoA availability and increases plant polyphenol synthesis . (unl.pt)

This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst. (kaist.ac.kr)

Broer S, Kramer R. Lysine uptake and exchange in Corynebacterium glutamicum. (atcc.org)
From zero to hero--design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. (academicjournals.org)
Deregulation of feedback inhibition of phosphoenolpyruvate carboxylase for improved lysine production in Corynebacterium glutamicum. (academicjournals.org)
Given its unique ability to co-utilize mixed carbon sources, C. glutamicum could be used to clean-up of wastes from agricultural or other industries, simultaneously producing useful compounds such as L-lysine or putrescine. (caister.com)
Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity. (semanticscholar.org)
Leucine synthesis in Corynebacterium glutamicum: enzyme activities, structure of leuA, and effect of leuA inactivation on lysine synthesis. (mysciencework.com)
Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. (uct.ac.za)
Fermentative Production of Lysine by Corynebacterium glutamicum from Different Carbon Sources', Iran Agricultural Research , 25.26(1.2), pp. 99-106. (ac.ir)
Production of lysine by Corynebacterium glutamicum (PTCC 1532) from different agricultural by-products (molasses and pulpy waste date) was compared to glucose as raw materials. (ac.ir)
An easily transferable metabolic engineering strategy for access of C. glutamicum to aldohexuronates was developed and applied to growth and production of the amino acids L-lysine and L-ornithine as well as the terpene lycopene from D-galacturonate or D-glucuronate. (springeropen.com)

Corynebacterium glutamicum is the prominent host for the fermentative production of amino acids with an average annual production volume of ~6 million tons. (mdpi.com)

Systems Metabolic Engineering of Corynebacterium glutamicum for Biobased Production of Chemicals, Materials and Fuels. (academicjournals.org)
CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum. (semanticscholar.org)

Sigma factors and promoters in Corynebacterium glutamicum. (semanticscholar.org)

Corynebacterium glutamicum , which is used in industrial amino acid production at a million-ton scale, has been engineered towards utilization of alternative carbon sources. (springeropen.com)
Utilization of the hexuronic acids D-galacturonic acid (abundant in pectin-rich waste streams such as peels and pulps) and D-glucuronic acid (a component of the side-chains of plant xylans) for growth and production with C. glutamicum has not yet been studied. (springeropen.com)

For the first time, we successfully achieved the production of the specialty anthocyanin C3G from the comparatively inexpensive flavonoid precursor catechin in C. glutamicum . (springer.com)
Another area of research into C. glutamicum is how to increase its production of L-glutamic acid, which is produced annually at a rate of about 1.5 million tons. (kenyon.edu)
This technique could be further developed, as well as transferred to increase production of other C. glutamicum products , such as other amino acids. (kenyon.edu)
Becker J, Wittmann C (2012) Bio-based production of chemicals, materials and fuels- Corynebacterium glutamicum as versatile cell factory. (springer.com)
Jojima T, Noburyu R, Suda M, Okino S, Yukawa H, Inui M. Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant Corynebacterium glutamicum . (mdpi.com)
The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. (asm.org)
Bio-based production of chemicals, materials and fuels-Corynebacterium glutamicum as versatile cell factory. (academicjournals.org)
Corynebacterium glutamicum tailored for high-yield L-valine production. (academicjournals.org)
C. glutamicum cells are frequently confronted with excessive reactive oxygen species (ROS) production, triggering sudden changes of fermentative conditions in temperature, pH, osmotic pressure, or toxic compounds [ 4 - 5 ]. (portlandpress.com)
Corynebacterium glutamicum is widely used for the industrial production of amino acids, nucleotides, and vitamins. (uio.no)
2007). Furthermore, within the last decade, C. glutamicum was applied in various production processes for fine chemicals, fuels and polymers. (caister.com)
Proteomics is an important technique to study the biology of Corynebacterium glutamicum with focus on processes related to the biotechnological production of amino acids and other chemicals. (caister.com)
Already metabolic engineering of only the terminal reactions leading to lycopene resulted in considerable lycopene production indicating that C. glutamicum may serve as a potential host for carotenoid production. (uni-bielefeld.de)
We successfully applied metabolic engineering strategies to optimize its synthetic production in the industrial workhorse C. glutamicum and thereby paved the way for further improvements in ectoine yield and biotechnological process optimization. (biomedcentral.com)
Engineering Corynebacterium glutamicum for the production of p. (mysciencework.com)
Engineering Corynebacterium glutamicum for the production of pyruvate. (mysciencework.com)
Biotin auxotrophy of C. glutamicum elicits L-glutamate production, a characteristic which led to its discovery. (biomedcentral.com)
A succinate efflux pump, SucE, was recently identified in C. glutamicum , which substantially increases succinate production when overexpressed. (shef.ac.uk)
Corynebacterium glutamicum ( C. glutamicum ) has traditionally been used as a microbial cell factory for the industrial production of many amino acids and other industrially important co. (biomedcentral.com)
L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicum . (springeropen.com)
Vol 6: Production of L-glutamic Acid with Corynebacterium glutamicum NCIM 2168 and Pseudomonas reptilivora NCIM 2598: A Study on Immobilization and Reusability. (duhnnae.com)
Corynebacterium glutamicum C. glutamicum is one of the major organisms widely used for glutamic acid production. (duhnnae.com)
In this study, the effects of oxygenation on C glutamicum growth and organic acids production were investigated through cultures in shake flask at different k(L)a conditions of 0.6, 3, 11, 19, 33, 36, 48, 77, 120 and 164 h(-1). (univ-lorraine.fr)
Our study also showed k(L)a ranges where C glutamicum growth was altered while organic acids production was improved. (univ-lorraine.fr)
Recently, efforts with C. glutamicum have shifted from optimizing production processes to also include access to alternative carbon sources. (springeropen.com)

To extend its product spectrum and improve productivity, C. glutamicum needs to undergo further engineering, including the development of applicable promoter system. (semanticscholar.org)
Here, we isolated new promoters from the fully synthetic promoter library consisting of 70-bp random sequences in C. glutamicum. (semanticscholar.org)

Here, we report that the ncgl2617 gene ( cosR ) of C. glutamicum encoding an MarR-type transcriptional regulator plays an important role in oxidative stress resistance. (portlandpress.com)

Handbook of Corynebacterium glutamicum. (academicjournals.org)

Mori and Shiio ( 43 ) first mentioned the presence of a phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) in C. glutamicum . (asm.org)

The characterization of methionine uptake in C. glutamicum revealed two uptake systems. (uni-koeln.de)
Comparative genome analyses suggested that the putative transport system BioYMN encoded by cg2147, cg2148 and cg2149 might be involved in biotin uptake by C. glutamicum . (biomedcentral.com)
Many bacteria can synthesize biotin, but biotin auxotrophic bacteria such as Corynebacterium glutamicum require uptake of biotin from the habitat. (biomedcentral.com)
C. glutamicum lacks an uptake system for the peptidoglycan constituent N-acetylglucosamine (GlcNAc), but is able to catabolize GlcNAc-6-phosphate. (biomedcentral.com)
tion systems of C. glutamicum impede its uptake of foreign DNA and thus limiting its transformation efficiency. (globalspec.com)

Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum. (semanticscholar.org)
Since most examples of regulation of gene expression in C . glutamicum describe transcriptional control, the main focus of this chapter is this level of regulation. (routledgehandbooks.com)

The established bioprocess is an elegant approach for the systematic understanding of C. glutamicum 's adaptation to a progressive oxygen deprivation. (aiche.org)
Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions. (microbiologyresearch.org)
Since the reaction occurred in the absence of cell growth, the ethanol volumetric productivity increased in proportion to cell density of ethanologenic C. glutamicum in a process under oxygen-deprivation conditions. (mendeley.com)

Corynebacterium the possibility of contamination in Soolingen D. Mycobacterium avium in diphtheriae is the agent of pharyngeal the environment by the aerosolized a shower linked to pulmonary disease. (cdc.gov)

Corynebacterium glutamicum (Kinoshita et al. (atcc.org)

Becker J, Giesselmann G, Hoffmann SL, Wittmann C (2018) Corynebacterium glutamicum for sustainable bioproduction: from metabolic physiology to systems metabolic engineering. (springer.com)

Amino sugars such as glucosamine or N-acetylglucosamine are potential alternative carbon sources for biotechnological processes with Corynebacterium glutamicum. (uni-koeln.de)

In the present work, methanethiol and dimethyldisulfide were investigated as sulfur source for methionine synthesis in Corynebacterium glutamicum. (nih.gov)
Leucine synthesis in Corynebacterium glutamicum: enzyme activi. (mysciencework.com)
The current work deals with the biotechnological synthesis of alpha-ketomethylvalerate (KMV) with Corynebacterium glutamicum. (uni-ulm.de)

Corynebacterium glutamicum was metabolically engineered to produce D-lactate from biomass. (asu.edu)
C. glutamicum was cultured with aromatics, such as vanillin, vanillic acid, and coumarate that are present in biomass hydrolysates and will be analyzed for the presence of D-lactate. (asu.edu)

For this purpose, the glycerophospholipidome of C. glutamicum grown at different temperatures was analyzed by mass spectrometry using quantitative multiple precursor ion scanning. (forskningsdatabasen.dk)
We have confirmed by mass spectrometry the absence of poly-β-hydrobutyrate (PHB) in C. glutamicum. (kribb.re.kr)
Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). (kaist.ac.kr)

Corynebacterium glutamicum grows on a variety of carbohydrates and organic acids. (asm.org)

Heterologous expression of exochitinase gene chiB from Serratia marcescens resulted in chitinolytic activity and ChiB secretion was enhanced when a signal peptide from C. glutamicum was used. (biomedcentral.com)

This database contains 8772 oligonucleotides to represent the Corynebacterium glutamicum R transcriptome. (umich.edu)

Anatomy1

Organisms9

Diseases2

Chemicals and Drugs34

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes18

Disciplines and Occupations2

Technology, Industry, Agriculture4

Information Science3

Evolutionary process of amino acid biosynthesis in Corynebacterium at the whole genome level. (1/403)

Purification and characterization of O-Acetylserine sulfhydrylase of Corynebacterium glutamicum. (2/403)

BetP of Corynebacterium glutamicum, a transporter with three different functions: betaine transport, osmosensing, and osmoregulation. (3/403)

Acyl-CoA carboxylases (accD2 and accD3), together with a unique polyketide synthase (Cg-pks), are key to mycolic acid biosynthesis in Corynebacterianeae such as Corynebacterium glutamicum and Mycobacterium tuberculosis. (4/403)

Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. (5/403)

Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease-encoding genes in Corynebacterium glutamicum. (6/403)

Integration of E. coli aroG-pheA tandem genes into Corynebacterium glutamicum tyrA locus and its effect on L-phenylalanine biosynthesis. (7/403)

Cometabolism of a nongrowth substrate: L-serine utilization by Corynebacterium glutamicum. (8/403)

Corynebacterium glutamicum

Corynebacterium

Corynebacterium Infections

Corynebacterium diphtheriae

Bacterial Proteins

Corynebacterium pseudotuberculosis

Gene Expression Regulation, Bacterial

Metabolic Engineering

Propionibacterium acnes

Genes, Bacterial

Gene Deletion

Mycolic Acids

Molecular Sequence Data

DNA, Bacterial

Brevibacterium

Oxaloacetic Acid

Biotechnology

Betaine

Operon

Lysine

Industrial Microbiology

Succinic Acid

Diphtheria

Corynebacterium pyogenes

Base Sequence

Genetic Complementation Test

Genetic Engineering

Gentisates

Amino Acids, Diamino

Escherichia coli

Amino Acid Sequence

Biosynthetic Pathways

Cloning, Molecular

Homoserine Dehydrogenase

Genome, Bacterial

RNA, Bacterial

Sequence Analysis, DNA

Culture Media

Hydroxybenzoates

Pentose Phosphate Pathway

Metabolic Networks and Pathways

Genetic Enhancement

Prephenate Dehydratase

Ketol-Acid Reductoisomerase

Pimelic Acids

Galactans

Plasmids

Sequence Homology, Amino Acid

Isoleucine

Electrophoretic Mobility Shift Assay

Malate Synthase

Mutation

Artificial Gene Fusion

Glucose

Valine

Glutamic Acid

Regulon

Chorismate Mutase

Membrane Transport Proteins

Glyceraldehyde 3-Phosphate

Promoter Regions, Genetic

Methylophilus methylotrophus

Isocitrate Lyase

Cell Wall

Diphtheria Toxin

Fermentation

Multigene Family

Phosphoenolpyruvate Sugar Phosphotransferase System

Transcription, Genetic

Pyruvic Acid

Phosphate Acetyltransferase

Glyoxylates

Repressor Proteins

Acetolactate Synthase

Mannosyltransferases

Evolutionary process of amino acid biosynthesis in Corynebacterium at the whole genome level. (1/403)

Purification and characterization of O-Acetylserine sulfhydrylase of Corynebacterium glutamicum. (2/403)

BetP of Corynebacterium glutamicum, a transporter with three different functions: betaine transport, osmosensing, and osmoregulation. (3/403)

Acyl-CoA carboxylases (accD2 and accD3), together with a unique polyketide synthase (Cg-pks), are key to mycolic acid biosynthesis in Corynebacterianeae such as Corynebacterium glutamicum and Mycobacterium tuberculosis. (4/403)

Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. (5/403)