Corynebacterium glutamicum
Corynebacterium
Corynebacterium diphtheriae
A species of gram-positive, asporogenous bacteria in which three cultural types are recognized. These types (gravis, intermedius, and mitis) were originally given in accordance with the clinical severity of the cases from which the different strains were most frequently isolated. This species is the causative agent of DIPHTHERIA.
Corynebacterium pseudotuberculosis
A species of gram-positive, asporogenous bacteria that was originally isolated from necrotic areas in the kidney of a sheep. It may cause ulcerative lymphangitis, abscesses, and other chronic purulent infections in sheep, horses, and other warm-blooded animals. Human disease may form from contact with infected animals.
Gene Expression Regulation, Bacterial
Metabolic Engineering
Propionibacterium acnes
Gene Deletion
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Brevibacterium
Oxaloacetic Acid
Biotechnology
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Betaine
A naturally occurring compound that has been of interest for its role in osmoregulation. As a drug, betaine hydrochloride has been used as a source of hydrochloric acid in the treatment of hypochlorhydria. Betaine has also been used in the treatment of liver disorders, for hyperkalemia, for homocystinuria, and for gastrointestinal disturbances. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1341)
Operon
Industrial Microbiology
Succinic Acid
A water-soluble, colorless crystal with an acid taste that is used as a chemical intermediate, in medicine, the manufacture of lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and a neutralizing agent. (Hawley's Condensed Chemical Dictionary, 12th ed, p1099; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1851)
Diphtheria
A localized infection of mucous membranes or skin caused by toxigenic strains of CORYNEBACTERIUM DIPHTHERIAE. It is characterized by the presence of a pseudomembrane at the site of infection. DIPHTHERIA TOXIN, produced by C. diphtheriae, can cause myocarditis, polyneuritis, and other systemic toxic effects.
Corynebacterium pyogenes
Base Sequence
Genetic Complementation Test
Genetic Engineering
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Amino Acid Sequence
Biosynthetic Pathways
Cloning, Molecular
Homoserine Dehydrogenase
RNA, Bacterial
Sequence Analysis, DNA
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Hydroxybenzoates
Pentose Phosphate Pathway
An oxidative decarboxylation process that converts GLUCOSE-6-PHOSPHATE to D-ribose-5-phosphate via 6-phosphogluconate. The pentose product is used in the biosynthesis of NUCLEIC ACIDS. The generated energy is stored in the form of NADPH. This pathway is prominent in tissues which are active in the synthesis of FATTY ACIDS and STEROIDS.
Metabolic Networks and Pathways
Genetic Enhancement
Prephenate Dehydratase
Ketol-Acid Reductoisomerase
Pimelic Acids
Galactans
Plasmids
Sequence Homology, Amino Acid
Isoleucine
Electrophoretic Mobility Shift Assay
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
Malate Synthase
Mutation
Artificial Gene Fusion
Glucose
Valine
Glutamic Acid
Regulon
Chorismate Mutase
Membrane Transport Proteins
Glyceraldehyde 3-Phosphate
Promoter Regions, Genetic
Methylophilus methylotrophus
Isocitrate Lyase
Cell Wall
Diphtheria Toxin
An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.
Fermentation
Multigene Family
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Phosphoenolpyruvate Sugar Phosphotransferase System
The bacterial sugar phosphotransferase system (PTS) that catalyzes the transfer of the phosphoryl group from phosphoenolpyruvate to its sugar substrates (the PTS sugars) concomitant with the translocation of these sugars across the bacterial membrane. The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. The PTS has also been implicated in the induction of synthesis of some catabolic enzyme systems required for the utilization of sugars that are not substrates of the PTS as well as the regulation of the activity of ADENYLYL CYCLASES. EC 2.7.1.-.
Transcription, Genetic
Pyruvic Acid
Phosphate Acetyltransferase
Repressor Proteins
Acetolactate Synthase
Evolutionary process of amino acid biosynthesis in Corynebacterium at the whole genome level. (1/403)
Corynebacterium glutamicum, which is the closest relative of Corynebacterium efficiens, is widely used for the large scale production of many kinds of amino acids, particularly glutamic acid and lysine, by fermentation. Corynebacterium diphtheriae, which is well known as a human pathogen, is also closely related to these two species of Corynebacteria, but it lacks such productivity of amino acids. It is an important and interesting question to ask how those closely related bacterial species have undergone such significant functional differentiation in amino acid biosynthesis. The main purpose of the present study is to clarify the evolutionary process of functional differentiation among the three species of Corynebacteria by conducting a comparative analysis of genome sequences. When Mycobacterium and Streptomyces were used as out groups, our comparative study suggested that the common ancestor of Corynebacteria already possessed almost all of the gene sets necessary for amino acid production. However, C. diphtheriae was found to have lost the genes responsible for amino acid production. Moreover, we found that the common ancestor of C. efficiens and C. glutamicum have acquired some of genes responsible for amino acid production by horizontal gene transfer. Thus, we conclude that the evolutionary events of gene loss and horizontal gene transfer must have been responsible for functional differentiation in amino acid biosynthesis of the three species of Corynebacteria. (+info)Purification and characterization of O-Acetylserine sulfhydrylase of Corynebacterium glutamicum. (2/403)
We highly purified O-acetylserine sulfhydrylase from the glutamate-producing bacterium Corynebacterium glutamicum. The molecular mass of the purified enzyme was 34,500 as determined by SDS-polyacrylamide gel electrophoresis, and 70,800 as determined by gel filtration chromatography. It had an apparent Km of 7.0 mM for O-acetylserine and a Vmax of 435 micromol min-1 (mg x protein)-1. This is the first report of the cysteine biosynthetic enzyme of C. glutamicum in purified form. (+info)BetP of Corynebacterium glutamicum, a transporter with three different functions: betaine transport, osmosensing, and osmoregulation. (3/403)
In order to circumvent deleterious effects of hypo- and hyperosmotic conditions in its environment, Corynebacterium glutamicum has developed a number of mechanisms to counteract osmotic stress. The first response to an osmotic upshift is the activation of uptake mechanisms for the compatible solutes betaine, proline, or ectoine, namely BetP, EctP, ProP, LcoP and PutP. BetP, the most important uptake system responds to osmotic stress by regulation at the level of both protein activity and gene expression. BetP was shown to harbor three different properties, i.e. catalytic activity (betaine transport), sensing of appropriate stimuli (osmosensing) and signal transduction to the catalytic part of the carrier protein which adapts its activity to the extent of osmotic stress (osmoregulation). BetP is comprised of 12 transmembrane segments and carries N- and C-terminal domains, which are involved in osmosensing and/or osmoregulation. Recent results on molecular properties of these domains indicate the significance of particular amino acids within the terminal 25 amino acids of the C-terminal domain of BetP for the process of osmosensing and osmoregulation. (+info)Acyl-CoA carboxylases (accD2 and accD3), together with a unique polyketide synthase (Cg-pks), are key to mycolic acid biosynthesis in Corynebacterianeae such as Corynebacterium glutamicum and Mycobacterium tuberculosis. (4/403)
The Corynebacterianeae such as Corynebacterium glutamicum and Mycobacterium tuberculosis possess several unique and structurally diverse lipids, including the genus-specific mycolic acids. Although the function of a number of genes involved in fatty acid and mycolic acid biosynthesis is known, information relevant to the initial steps within these biosynthetic pathways is relatively sparse. Interestingly, the genomes of Corynebacterianeae possess a high number of accD genes, whose gene products resemble the beta-subunit of the acetyl-CoA carboxylase of Escherichia coli, providing the activated intermediate for fatty acid synthesis. We present here our studies on four putative accD genes found in C. glutamicum. Although growth of the accD4 mutant remained unchanged, growth of the accD1 mutant was strongly impaired and partially recovered by the addition of exogenous oleic acid. Overexpression of accD1 and accBC, encoding the carboxylase alpha-subunit, resulted in an 8-fold increase in malonyl-CoA formation from acetyl-CoA in cell lysates, providing evidence that accD1 encodes a carboxyltransferase involved in the biosynthesis of malonyl-CoA. Interestingly, fatty acid profiles remained unchanged in both our accD2 and accD3 mutants, but a complete loss of mycolic acids, either as organic extractable trehalose and glucose mycolates or as cell wall-bound mycolates, was observed. These two carboxyltransferases are also retained in all Corynebacterianeae, including Mycobacterium leprae, constituting two distinct groups of orthologs. Furthermore, carboxyl fixation assays, as well as a study of a Cg-pks deletion mutant, led us to conclude that accD2 and accD3 are key to mycolic acid biosynthesis, thus providing a carboxylated intermediate during condensation of the mero-chain and alpha-branch directed by the pks-encoded polyketide synthase. This study illustrates that the high number of accD paralogs have evolved to represent specific variations on the well known basic theme of providing carboxylated intermediates in lipid biosynthesis. (+info)Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. (5/403)
In Corynebacterium glutamicum, the activity of aconitase is 2.5-4-fold higher on propionate, citrate, or acetate than on glucose. Here we show that this variation is caused by transcriptional regulation. In search for putative regulators, a gene (acnR) encoding a TetR-type transcriptional regulator was found to be encoded immediately downstream of the aconitase gene (acn) in C. glutamicum. Deletion of the acnR gene led to a 5-fold increased acn-mRNA level and a 5-fold increased aconitase activity, suggesting that AcnR functions as repressor of acn expression. DNA microarray analyses indicated that acn is the primary target gene of AcnR in the C. glutamicum genome. Purified AcnR was shown to be a homodimer, which binds to the acn promoter in the region from -11 to -28 relative to the transcription start. It thus presumably acts by interfering with the binding of RNA polymerase. The acn-acnR organization is conserved in all corynebacteria and mycobacteria with known genome sequence and a putative AcnR consensus binding motif (CAGNACnnncGTACTG) was identified in the corresponding acn upstream regions. Mutations within this motif inhibited AcnR binding. Because the activities of citrate synthase and isocitrate dehydrogenase were previously reported not to be increased during growth on acetate, our data indicate that aconitase is a major control point of tricarboxylic acid cycle activity in C. glutamicum, and they identify AcnR as the first transcriptional regulator of a tricarboxylic acid cycle gene in the Corynebacterianeae. (+info)Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease-encoding genes in Corynebacterium glutamicum. (6/403)
The molecular identification of the Corynebacterium glutamicum urea uptake system is described. This ABC-type transporter is encoded by the urtABCDE operon, which is transcribed in response to nitrogen limitation. Expression of the urt genes is regulated by the global nitrogen regulator AmtR, and an amtR deletion strain showed constitutive expression of the urtABCDE genes. The AmtR repressor protein also controls transcription of the urease-encoding ureABCEFGD genes in C. glutamicum. The ure gene cluster forms an operon which is mainly transcribed in response to nitrogen starvation. To confirm the increased synthesis of urease subunits under nitrogen limitation, proteome analyses of cytoplasmic protein extracts from cells grown under nitrogen surplus and nitrogen limitation were carried out, and five of the seven urease subunits were identified. (+info)Integration of E. coli aroG-pheA tandem genes into Corynebacterium glutamicum tyrA locus and its effect on L-phenylalanine biosynthesis. (7/403)
AIM: To study the effect of integration of tandem aroG-pheA genes into the tyrA locus of Corynebacterium glutamicum (C. glutamicum) on the production of L-phenylalanine. METHODS: By nitrosoguanidine mutagenesis, five p-fluorophenylalanine (FP)-resistant mutants of C.glutamicum FP were selected. The tyrA gene encoding prephenate dehydrogenase (PDH) of C.glutamicum was amplified by polymerase chain reaction (PCR) and cloned on the plasmid pPR. Kanamycin resistance gene (Km) and the P(BF) -aroG-pheA-T (GA) fragment of pGA were inserted into tyrA gene to form targeting vectors pTK and pTGAK, respectively. Then, they were transformed into C.glutamicum FP respectively by electroporation. Cultures were screened by a medium containing kanamycin and detected by PCR and phenotype analysis. The transformed strains were used for L-phenylalanine fermentation and enzyme assays. RESULTS: Engineering strains of C.glutamicum (Tyr(-)) were obtained. Compared with the original strain, the transformed strain C. glutamicum GAK was observed to have the highest elevation of L-phenylalanine production by a 1.71-fold, and 2.9-, 3.36-, and 3.0-fold in enzyme activities of chorismate mutase, prephenate dehydratase and 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase, respectively. CONCLUSION: Integration of tandem aroG-pheA genes into tyrA locus of C. glutamicum chromosome can disrupt tyrA gene and increase the yield of L-phenylalanine production. (+info)Cometabolism of a nongrowth substrate: L-serine utilization by Corynebacterium glutamicum. (8/403)
Despite its key position in central metabolism, L-serine does not support the growth of Corynebacterium glutamicum. Nevertheless, during growth on glucose, L-serine is consumed at rates up to 19.4 +/- 4.0 nmol min(-1) (mg [dry weight])(-1), resulting in the complete consumption of 100 mM L-serine in the presence of 100 mM glucose and an increased growth yield of about 20%. Use of 13C-labeled L-serine and analysis of cellularly derived metabolites by nuclear magnetic resonance spectroscopy revealed that the carbon skeleton of L-serine is mainly converted to pyruvate-derived metabolites such as L-alanine. The sdaA gene was identified in the genome of C. glutamicum, and overexpression of sdaA resulted in (i) functional L-serine dehydratase (L-SerDH) activity, and therefore conversion of L-serine to pyruvate, and (ii) growth of the recombinant strain on L-serine as the single substrate. In contrast, deletion of sdaA decreased the L-serine cometabolism rate with glucose by 47% but still resulted in degradation of L-serine to pyruvate. Cystathionine beta-lyase was additionally found to convert L-serine to pyruvate, and the respective metC gene was induced 2.4-fold under high internal L-serine concentrations. Upon sdaA overexpression, the growth rate on glucose is reduced 36% from that of the wild type, illustrating that even with glucose as a single substrate, intracellular L-serine conversion to pyruvate might occur, although probably the weak affinity of L-SerDH (apparent Km, 11 mM) prevents substantial L-serine degradation. (+info)
Domain assignment for gi|145295828|ref|YP 001138649.1| from Corynebacterium glutamicum R
Corynebacterium glutamicum - Wikipedia
The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and...
Large-Scale Engineering of the Corynebacterium glutamicum Genome | Applied and Environmental Microbiology
Handbook of Corynebacterium glutamicum
Handbook of Corynebacterium glutamicum
dcd - dCTP deaminase, dUMP-forming - Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG...
ureB - Urease subunit beta - Corynebacterium glutamicum (strain R) - ureB gene & protein
Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate...
Der Regulationsmechanismus des Osmosensors BetP aus Corynebacterium glutamicum - Kölner UniversitätsPublikationsServer
Efflux permease CgAcr3-1 of Corynebacterium glutamicum is an arsenite-specific antiporter. | SBRC
Analysis of the biotechnological production of 2-ketomethylvalerate with Corynebacterium glutamicum
Isolation of plasmid DNA from Corynebacterium glutamicum using the QIAGEN Plasmid Mini Kit
Improvement of l -ornithine production by attenuation of argF in engineered Corynebacterium glutamicum S9114 | AMB Express |...
Frontiers | Efficient Production of the Dicarboxylic Acid Glutarate by Corynebacterium glutamicum via a Novel Synthetic Pathway...
CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum | Biochemical Journal |...
Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum | Microbial Cell Factories | Full Text
Molecular cloning of the Corynebacterium glutamicum (Brevibacterium lactofermentum AJ12036) odhA gene encoding a novel type...
RCSB PDB - 2WIT: CRYSTAL STRUCTURE OF THE SODIUM-COUPLED GLYCINE BETAINE SYMPORTER BETP FROM CORYNEBACTERIUM GLUTAMICUM WITH...
RNA-guided single/double gene repressions in Corynebacterium glutamicum using an efficient CRISPR interference and its...
Fermentative Production of Lysine by Corynebacterium glutamicum from Different Carbon Sources
Spontaneous induction of cryptic prophages in populations of the model species Corynebacterium glutamicum and Escherichia coli ...
Corynebacterium glutamicum catalase-gold nanoparticles nanostructured material
mntR - 196627 v2020 s21 dsRNA - Abasy Atlas
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Lysine production from the sugar alcohol mannitol: Design of the cell factory Corynebacterium glutamicum SEA-3 through...
INBIOTEC welcomes relevant researchers on amino acids production | Inbiotec
Corynebacterium glutamicum: From Systems Biology to Biotechnological Applications
Two-Component Systems of Corynebacterium glutamicum: Deletion Analysis and Involvement of the PhoS-PhoR System in the Phosphate...
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Polymerization Dynamics of the Prophage-Encoded Actin-Like Protein AlpC Is Influenced by the DNA-Binding Adapter AlpA
Corynebacterineae - Wikipedia
Der Glycinbetain-Transporter BetP aus Corynebacterium glutamicum - Kölner UniversitätsPublikationsServer
pCGR2 copy number depends on the par locus that forms a ParC-ParB-DNA partition complex in Corynebacterium glutamicum<...
L-citrulline production by metabolically engineered Corynebacterium glutamicum from glucose and alternative carbon sources |...
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Corynebacterium glutamicum 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) GENTAUR-58b8a5990ec21 | Gentaursearch
Corynebacterium glutamicum possesses β-N-acetylglucosaminidase | BMC Microbiology | Full Text
Altered Metabolic Flux due to Deletion of odhA causes l-Glutamate Overproduction in Corynebacterium glutamicum | Applied and...
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Volutin granules
"Formation of volutin granules in Corynebacterium glutamicum". FEMS Microbiology Letters. Institut fr Biotechnologie (IBT-1), ... the production of which is used as one of the identifying criteria when attempting to isolate Corynebacterium diphtheriae on ...
Essential gene
Suzuki N, Inui M, Yukawa H (2011). High-Throughput Transposon Mutagenesis of Corynebacterium glutamicum. Methods in Molecular ...
Brevibacterium
... flavum is now classified as Corynebacterium glutamicum. Brevibacterium stationis may now be re-classified as ... Yangyong Lv (Feb 2012). "Genome Sequence of Corynebacterium glutamicum ATCC 14067, Which Provides Insight into Amino Acid ... as Corynebacterium stationis comb. nov., and emended description of the genus Corynebacterium to include isolates that can ... Corynebacterium stationis. Mimura, Haruo (September 2014). "Growth Enhancement of the Halotolerant "Brevibacterium" sp JCM 6894 ...
Corynebacterial porin B
Porins B and C are cell wall channel-forming proteins from Corynebacterium. Porin B from Corynebacterium glutamicum ( ... Ziegler K, Benz R, Schulz GE (June 2008). "A putative alpha-helical porin from Corynebacterium glutamicum". J. Mol. Biol. 379 ( ...
Isocitrate dehydrogenase
Chen R, Yang H (November 2000). "A highly specific monomeric isocitrate dehydrogenase from Corynebacterium glutamicum". Arch. ... C. glutamicum favored NADP+ over NAD+. In terms of stability with response to temperature, both enzymes had a similar Tm or ... glutamicum was recorded as having ten times as much activity than E. coli and seven times more affinitive/specific for NADP. ... glutamicum and E. coli, monomer and dimer, respectively, both enzymes were found to "efficiently catalyze identical reactions ...
Oxaloacetate decarboxylase
Jetten MS, Sinskey AJ (1995). "Purification and properties of oxaloacetate decarboxylase from Corynebacterium glutamicum". ... decarboxylase from the family of divalent cation dependent decarboxylases was isolated from Corynebacterium glutamicum in 1995 ... Found in different microorganisms such as Pseudomonas, Acetobacter, C. glutamicum, Veillonella parvula, and A. vinelandii, ...
Protein production
Date M, Itaya H, Matsui H, Kikuchi Y (January 2006). "Secretion of human epidermal growth factor by Corynebacterium glutamicum ... "The transcriptional regulatory repertoire of Corynebacterium glutamicum: reconstruction of the network controlling pathways ... The C. glutamicum species is widely used for producing glutamate and lysine, components of human food, animal feed and ... Non-pathogenic species of the gram-positive Corynebacterium are used for the commercial production of various amino acids. ...
Homoserine dehydrogenase
"Characteristics of methionine production by an engineered Corynebacterium glutamicum strain". Metab. Eng. 9 (4): 327-336. doi: ...
Amino acid synthesis
... its regulation and biotechnological application in Corynebacterium glutamicum". Microbial Biotechnology. 7 (1): 5-25. doi: ...
Histidine
"Modification of histidine biosynthesis pathway genes and the impact on production of L-histidine in Corynebacterium glutamicum ... its regulation and biotechnological application in Corynebacterium glutamicum". Microbial Biotechnology. 7 (1): 5-25. doi: ...
CtRNA
In Corynebacterium glutamicum, it achieves this by antisense pairing with the mRNA of RepB, a replication initiation protein. ... "Control of rep gene expression in plasmid pGA1 from Corynebacterium glutamicum". J Bacteriol. 185 (8): 2402-2409. doi:10.1128/ ...
Transsulfuration pathway
Hwang, B. J.; Yeom, H. J.; Kim, Y.; Lee, H. S. (2002). "Corynebacterium glutamicum utilizes both transsulfuration and direct ...
Xerlin
Metabolic Engineering of the Valine Pathway in Corynebacterium Glutamicum - Analysis and Modelling. Germany: Forschungszentrum ...
Minicell
"Generation of Minicells from an Endotoxin-Free Gram-Positive Strain Corynebacterium glutamicum". Journal of Microbiology and ...
Industrial microbiology
The production of these amino acids is due to Corynebacterium glutamicum and fermentation. C.glutamicum was engineered to be ... L-Lysine was originally produced from diaminopimelic acid (DAP) by E.coli, but once the C.glutamicum was discovered for the ... L-Tryptophan is also produced through fermentation and by Corynebacterium and E.coli, though the production is not as large as ... "Corynebacterium species , Johns Hopkins ABX Guide". www.hopkinsguides.com. Retrieved 2019-11-11. Singhania, Reeta Rani; Patel, ...
Porin (protein)
... a channel forming peptide from Corynebacterium glutamicum". FEBS Letters. 587 (22): 3687-91. doi:10.1016/j.febslet.2013.09.032 ...
Glutamate flavoring
Manufacturers, such as Ajinomoto, use selected strains of Corynebacterium glutamicum bacteria in a nutrient-rich medium. The ...
LysC
It is found in a variety of bacteria, including Bacillus subtilis, Escherichia coli and Corynebacterium glutamicum. It is ...
Lysine
The industrial process includes the fermentative culturing of Corynebacterium glutamicum and the subsequent purification of ... "Development of an L-Lysine Enriched Bran for Animal Nutrition via Submerged Fermentation by Corynebacterium glutamicum using ...
Lysine exporter
The superfamily was named based on the early discovery of the LysE carrier protein of Corynebacterium glutamicum. 2.A.75 - The ... LysE of Corynebacterium glutamicum (TC# 2.A.75.1.1) and ArgO of E. coli) have been functionally characterized, but functionally ... topology of the lysine exporter LysE of Corynebacterium glutamicum, a paradyme for a novel superfamily of transmembrane solute ... L-lysine export from Corynebacterium glutamicum". Molecular Microbiology. 22 (5): 815-26. doi:10.1046/j.1365-2958.1996.01527.x ...
Pyruvate dehydrogenase complex
"E1 Enzyme of the Pyruvate Dehydrogenase Complex in Corynebacterium glutamicum: Molecular Analysis of the Gene and Phylogenetic ...
Streptomyces mobaraensis
"High level expression of Streptomyces mobaraensis transglutaminase in Corynebacterium glutamicum using a chimeric pro-region ...
Basfia succiniciproducens
... and Corynebacterium glutamicum. Metabolic engineering for the production of high quantities of succinic acid should be ...
Fed-batch culture
An example is lysine production with homoserine- or threonine/methionine-requiring mutant of Corynebacterium glutamicum being ...
Cyanophycin
... of cyanophycin in a number of biotechnologically relevant bacteria such as Escherichia coli and Corynebacterium glutamicum. ...
Z curve
"A systematic method to identify genomic islands and its applications in analyzing the genomes of Corynebacterium glutamicum and ...
Bglg-cis-reg RNA
... the difference in glucose repression of two β-glucoside phosphotransferase system gene clusters in Corynebacterium glutamicum R ...
Microbiology
Corynebacterium glutamicum is one of the most important bacterial species with an annual production of more than two million ...
Succinic acid fermentation
Corynebacterium glutamicum and Saccharomyces cerevisia. Understanding of the central carbon metabolism of these organisms is ...
Galactan 5-O-arabinofuranosyltransferase
... octacis-decaprenol This enzyme is isolated from Mycobacterium tuberculosis and Corynebacterium glutamicum. Alderwick LJ, Seidel ...
Lizin - Wikipedija, prosta enciklopedija
Industrijsko se L-lizin običajno izdeluje s fermentacijo ob pomoči Corynebacterium glutamicum ; proizvodnja presega 600.000 ton ...
Glutamic acid
... with the organism Corynebacterium glutamicum (also known as Brevibacterium flavum) being the most widely used for production.[ ...
Histidine
... its regulation and biotechnological application in Corynebacterium glutamicum". Microbial Biotechnology. 7 (1): 5-25. doi: ... "Modification of histidine biosynthesis pathway genes and the impact on production of L-histidine in Corynebacterium glutamicum ...
Streptomyces isolates
... used to produce a number of fermented soy products Biotechnology in pharmaceutical manufacturing Corynebacterium glutamicum, ...
Triptofaan
August 2012) "Bio-based production of chemicals, materials and fuels -Corynebacterium glutamicum as versatile cell factory". ... glutamicum of E. coli. Partykeer word geneties gemodifiseerde bakterië gebruik. Die bakterië wat gebruik word het almal ...
Triptófano, a enciclopedia libre
Corynebacterium glutamicum ou Escherichia coli. Estas estirpes bacterianas teñen mutacións que impiden a recaptación de ...
ක්ෂුද්රජීව විද්යාව - විකිපීඩියා
ඇමයිනෝ අම්ල ආශ්රිත කර්මාන්ත වලදී Corynebacterium glutamicum නම් බැක්ටීරියා වැදගත් දායකත්වයක් දරනු ලබයි.එමඟින් වාර්ෂිකව ටොන් ...
Protein production
Date M, Itaya H, Matsui H, Kikuchi Y (January 2006). "Secretion of human epidermal growth factor by Corynebacterium glutamicum ... Corynebacterium[edit]. Non-pathogenic species of the gram-positive Corynebacterium are used for the commercial production of ... "The transcriptional regulatory repertoire of Corynebacterium glutamicum: reconstruction of the network controlling pathways ... The C. glutamicum species is widely used for producing glutamate and lysine,[14] components of human food, animal feed and ...
කාර්මික ක්ෂුද්ර ජීව විද්යාව - විකිපීඩියා, නිදහස් විශ්වකෝෂය
Corynebacterium glutamicum (කොරයින\බැක්ටීරියm, ග්ලූටැමීkම්) යනු වාර්ෂික නිෂ්පාදනය ටොන් මිලියන දෙකක් පමණ වන ඇමයිනෝ අම්ල ...
Corynebacterium glutamicum
Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. Corynebacterium glutamicum ATCC 14067 was ... Genome sequence for C. glutamicum from NCBI. Type strain of Corynebacterium glutamicum at BacDive - the Bacterial Diversity ... Corynebacterium glutamicum (previously known as Micrococcus glutamicus) is a Gram-positive, rod-shaped bacterium that is used ... 4 September 2003). "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l- ...
Nathan H. Lents
... conducting basic microbiology research on the soil bacteria Corynebacterium glutamicum, which is used in the production of ...
Corynebacterium efficiens
"Genomic analyses of transporter proteins in Corynebacterium glutamicum and Corynebacterium efficiens". In Eggeling, Lothar; ... "The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium ... "Corynebacterium efficiens" at the Encyclopedia of Life LPSN Type strain of Corynebacterium efficiens at BacDive - the Bacterial ... Handbook of Corynebacterium glutamicum. pp. 149-86. ISBN 978-1-4200-3969-6. Zhang, R.; Zhang, C.-T. (2005). "Genomic Islands in ...
Corynebacterium glutamicum - Wikipedia
Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. Corynebacterium glutamicum ATCC 14067 was ... Genome sequence for C. glutamicum from NCBI. Type strain of Corynebacterium glutamicum at BacDive - the Bacterial Diversity ... Corynebacterium glutamicum (previously known as Micrococcus glutamicus) is a Gram-positive, rod-shaped bacterium that is used ... 4 September 2003). "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l- ...
Corynebacterium glutamicum - Biology and Biotechnology | Masayuki Inui | Springer
... glutamicum. Understanding its cell physiology will help manufacturers to increase their product range and productivity through ... Pathways at Work: Metabolic Flux Analysis of the Industrial Cell Factory Corynebacterium glutamicum ... genome engineering tools and comprehensive analysis techniques for Corynebacterium glutamicum. Aside from modern omics-based ... Newly added topics include cell envelope structures and aromatic compound metabolism in C. glutamicum. These chapters will be ...
Metabolic engineering of Corynebacterium glutamicum for anthocyanin production | SpringerLink
Engineering Corynebacterium glutamicum for isobutanol production. Appl Microbiol Biotechnol. 2010;87:1045-55.CrossRefPubMed ... Metabolic engineering of Corynebacterium glutamicum for l-arginine production. Nat Commun. 2014;5:4618.CrossRefPubMedGoogle ... Corynebacterium glutamicum, having been widely used in industrial production of amino acids such as L-glutamate and l-lysine [ ... Bio-based production of organic acids with Corynebacterium glutamicum. Microb Biotechnol. 2013;6:87-102.CrossRefPubMedGoogle ...
cg2901 hypothetical protein [Corynebacterium glutamicum ATCC 13032] - Gene - NCBI
cg2901 [Corynebacterium glutamicum ATCC 13032] cg2901 [Corynebacterium glutamicum ATCC 13032]. Gene ID:3345093 ... cg2901 hypothetical protein [ Corynebacterium glutamicum ATCC 13032 ] Gene ID: 3345093, discontinued on 9-Feb-2015 * All Gene ... Corynebacterium glutamicum ATCC 13032 (strain: DSM 20300; ATCC 13032) Lineage. Bacteria; Actinobacteria; Actinobacteridae; ... Actinomycetales; Corynebacterineae; Corynebacteriaceae; Corynebacterium. NEW Try the new Gene table Try the new Transcript ...
Corynebacterium glutamicum | Journal of Bacteriology
PcaO Positively Regulates pcaHG of the β-Ketoadipate Pathway in Corynebacterium glutamicum Ke-Xin Zhao, Yan Huang, Xi Chen, Nan ... Polynucleotide Phosphorylase, RNase E/G, and YbeY Are Involved in the Maturation of 4.5S RNA in Corynebacterium glutamicum ... Characterization of the Dicarboxylate Transporter DctA in Corynebacterium glutamicum Jung-Won Youn, Elena Jolkver, Reinhard ... Subcellular Localization and Characterization of the ParAB System from Corynebacterium glutamicum Catriona Donovan, Astrid ...
lipA lipoyl synthase [Corynebacterium glutamicum ATCC 13032] - Gene - NCBI
lipA [Corynebacterium glutamicum ATCC 13032] lipA [Corynebacterium glutamicum ATCC 13032]. Gene ID:3345051 ... lipA lipoyl synthase [ Corynebacterium glutamicum ATCC 13032 ] Gene ID: 3345051, discontinued on 9-Feb-2015 * All Gene records ... Corynebacterium glutamicum ATCC 13032 (strain: DSM 20300; ATCC 13032) Lineage. Bacteria; Actinobacteria; Actinobacteridae; ... Actinomycetales; Corynebacterineae; Corynebacteriaceae; Corynebacterium. NEW Try the new Gene table Try the new Transcript ...
Corynebacterium glutamicum - microbewiki
Corynebacterium glutamicum Description and significance. C. glutamicum is a small, non-moving Gram-positive soil bacterium. ... "Corynebacterium glutamicum as a model bacterium for the bioremediation of arsenic". International Microbiology. 2006. p. 207- ... "Reduced Folate Supply as a Key to Enhanced L-Serine Production by Corynebacterium glutamicum." Applied and Environmental ... Another possible use for C. glutamicum is in bioremediation, such as for arsenic. C. glutamicum contains two operons in its ...
Synthetic biology approaches to access renewable carbon source utilization in Corynebacterium glutamicum | SpringerLink
... glutamicum), an important industrial workhorse, is capable of efficiently producing a variety of value-added chemicals and ... Corynebacterium glutamicum (C. glutamicum), an important industrial workhorse, is capable of efficiently producing a variety of ... Corynebacterium glutamicum Renewable sources Synthetic biology Metabolic engineering This is a preview of subscription content ... Yang J, Zhu Y, Men Y, Sun S, Zeng Y, Zhang Y, Sun Y, Ma Y (2016) Pathway construction in Corynebacterium glutamicum and strain ...
RCSB PDB - 5H2Y: Crystal strcuture of reduced DapF from Corynebacterium glutamicum
Corynebacterium glutamicum ATCC 13032. Mutation(s): 0 Gene Names: dapF, Cgl1943, cg2129. EC: 5.1.1.7. ... Find proteins for Q8NP73 (Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)) ... Here, we report the crystal structure of DapF from Corynebacterium glutamicum (CgDapF). Structures of CgDapF obtained under ... Structural basis for redox sensitivity in Corynebacterium glutamicum diaminopimelate epimerase: an enzyme involved in l-lysine ...
Genes | Free Full-Text | Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum
Corynebacterium glutamicum is the prominent host for the fermentative production of amino acids with an average annual ... Due to its robustness and well established large-scale fermentation, C. glutamicum has been engineered for the production of a ... In this study, systematic metabolic engineering enabled construction of a patchoulol producing C. glutamicum strain by applying ... Corynebacterium glutamicum; sesquiterpene; metabolic engineering; algae; Escherichia coli patchoulol; Corynebacterium ...
Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum | Nature...
provide insights into the roles of DNA partitioning protein ParB and SMC condensin complexes in Corynebacterium glutamicum. ... the role of DNA-partitioning protein ParB and SMC condensin complexes in the actinobacterium Corynebacterium glutamicum. ... 36 to unveil the global organization of the diploid Corynebacterium glutamicum genome. C. glutamicum is a polar growing ... 3: Functional characterization of two SMC-like complexes in C. glutamicum.. a Sections of the C. glutamicum genome map ...
Phenol Biodegradation by Corynebacterium glutamicum Encapsulated in Electrospun Fibers
... developed a model system for the biodegradation of phenol-contaminated wastewater by the bacterium Corynebacterium glutamicum. ... A. Nardi, R. Avrahami, E. Zussman, J. Rokem and C. Greenblatt, "Phenol Biodegradation by Corynebacterium glutamicum ... Is Involved in Intercellular Communication of Corynebacterium glutamicum," Archives of Microbiology, Vol. 182, No. 4, 2004, pp ... developed a model system for the biodegradation of phenol-contaminated wastewater by the bacterium Corynebacterium glutamicum. ...
lexA - LexA repressor - Corynebacterium glutamicum ATCC 14067 - lexA gene & protein
Corynebacterium glutamicum (strain R). Corynebacterium glutamicum (Brevibacterium saccharolyticum). Corynebacterium glutamicum ... Corynebacterium glutamicum ATCC 14067. Corynebacterium crenatum MT. [Brevibacterium] flavum. Corynebacterium glutamicum (strain ... Corynebacterium glutamicum (Brevibacterium saccharolyticum). Corynebacterium glutamicum ATCC 14067. Corynebacterium crenatum MT ... Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025). Corynebacterium glutamicum ( ...
pin - DNA recombinase homolog Pin - Corynebacterium glutamicum - pin gene & protein
DNA binding by Corynebacterium glutamicum TetR-type transcription regulator AmtR
Corynebacterium glutamicum (Kinoshita et al.) Abe et al. ATCC ® 13
Corynebacterium glutamicum ATCC ® 13032™ Designation: 534 TypeStrain=True Application: Produces glutamic acid L-glutamate, L- ... Nucleotide (GenBank) : Y13221 Corynebacterium glutamicum glnA gene. Nucleotide (GenBank) : AJ238250 Corynebacterium glutamicum ... Nucleotide (GenBank) : Y17592 Corynebacterium glutamicum accDA gene. Nucleotide (GenBank) : AJ012293 Corynebacterium glutamicum ... Corynebacterium glutamicum, gdh gen for glutamate dehydrogenase. Nucleotide (GenBank) : U31281 Corynebacterium glutamicum ...
Frontiers | Interaction sites of DivIVA and RodA from Corynebacterium glutamicum | Microbiology
We previously described a role of RodA from Corynebacterium glutamicum in apical cell growth and morphogenesis. Deletion of ... We previously described a role of RodA from Corynebacterium glutamicum in apical cell growth and morphogenesis. Deletion of ... Efficient electrotransformation of Corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum ... Eikmanns, B. J., Kleinertz, E., Liebl, W., and Sahm, H. (1991). A family of Corynebacterium glutamicum/Escherichia coli shuttle ...
Corynebacterium glutamicum | LGC Standards
RCSB PDB - 3LMD: Crystal structure of geranylgeranyl pyrophosphate synthase from corynebacterium glutamicum atcc 13032
Corynebacterium glutamicum. Mutation(s): 0 Gene Names: ispB, Cgl0473, cg0559. EC: 2.5.1.30. ... Find proteins for Q8NT37 (Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)) ... Crystal Structure of Geranylgeranyl Pyrophosphate Synthase from Corynebacterium Glutamicum. Patskovsky, Y., Ho, M., Toro, R., ... Crystal structure of geranylgeranyl pyrophosphate synthase from corynebacterium glutamicum atcc 13032. *DOI: 10.2210/pdb3LMD/ ...
New Trends in Corynebacterium glutamicum: Beyond the Amino Acids - Nova Science Publishers
New Trends in Corynebacterium glutamicum: Beyond the Amino Acids. Carlos Barreiro (Editor). Instituto de Biotecnología de León ... Use of Corynebacterium glutamicum for Heavy Metals Bioremediation: Arsenic as Prototype. (Almudena F. Villadangos, José A. Gil ... Home / Shop / Books / Science and Technology / Life Sciences / New Trends in Corynebacterium glutamicum: Beyond the Amino Acids ... L-Valine production in Corynebacterium glutamicum. (Kazumi Hiraga, Crispinus A. Omumasaba, Masayuki Inui, Research Institute of ...
Forschungszentrum Jülich - Announcements, honors, awards - An energetic profile of Corynebacterium glutamicum
An energetic profile of Corynebacterium glutamicum. All processes in microorganisms require energy, primarily provided in form ... Physiology and global gene expression of a Corynebacterium glutamicum ΔF(1)F(O)-ATP synthase mutant devoid of oxidative ... An energetic profile of Corynebacterium glutamicum underpinned by measured biomass yield on ATP. Metabolic Engineeering, 2021, ... In a study, the in vivo ATP yield of C. glutamicum is determined for the first time. This was possible by constructing an ATP ...
Advancing Dual-Phase Production Strategies with Corynebacterium Glutamicum through Systems Biology | SBE | Society for...
Advancing Dual-Phase Production Strategies with Corynebacterium Glutamicum through Systems Biology. *Conference: Metabolic ... To address this question, we systematically investigate the adaptation of the industrially relevant Corynebacterium glutamicum ... Conclusion. The established bioprocess is an elegant approach for the systematic understanding of C. glutamicums adaptation to ... Methods. A "triple-phase" batch bioprocess with C. glutamicum was established that depicts the three successive phases ( ...
The DeoR-Type Regulator SugR Represses Expression of ptsG in Corynebacterium glutamicum | Journal of Bacteriology
The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium ... Corynebacterium glutamicum is a high-G+C content gram-positive soil bacterium which was isolated in 1957 as an l-glutamate- ... The DeoR-Type Regulator SugR Represses Expression of ptsG in Corynebacterium glutamicum Verena Engels, Volker F. Wendisch ... Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. J. Biol. Chem. 280:585- ...
Isolation of plasmid DNA from Corynebacterium glutamicum using the QIAGEN Plasmid Mini Kit
Fermentation | Free Full-Text | Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant...
... glutamicum under the given conditions. However, glucose consumption for cell preparation decreases process yield (defined as ... Aerobically cultivated cells of Corynebacterium glutamicum produce mixed organic acids, including succinic acid (SA), from ... Keywords: Corynebacterium glutamicum; oxygen deprivation; succinic acid; cell recycle reaction Corynebacterium glutamicum; ... Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant Corynebacterium glutamicum. Toru Jojima 1 ...
A Novel Corynebacterium glutamicum l-Glutamate Exporter | Applied and Environmental Microbiology
Amino acid exporters in Corynebacterium glutamicum, p 335-349. In Yukawa H, Inui M (ed), Corynebacterium glutamicum. Springer, ... A Novel Corynebacterium glutamicum l-Glutamate Exporter. Yu Wang, Guoqiang Cao, Deyu Xu, Liwen Fan, Xinyang Wu, Xiaomeng Ni, ... A Novel Corynebacterium glutamicum l-Glutamate Exporter. Yu Wang, Guoqiang Cao, Deyu Xu, Liwen Fan, Xinyang Wu, Xiaomeng Ni, ... A Novel Corynebacterium glutamicum l-Glutamate Exporter. Yu Wang, Guoqiang Cao, Deyu Xu, Liwen Fan, Xinyang Wu, Xiaomeng Ni, ...
African Journal of Biotechnology - metabolic engineering of corynebacterium glutamicum to enhance l-leucine production
Key words: Corynebacterium glutamicum, L-leucine, metabolic engineering, fermentation, industrial production. ... Key words: Corynebacterium glutamicum, L-leucine, metabolic engineering, fermentation, industrial production. ... A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase ( ... A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase ( ...
Large-Scale Engineering of the Corynebacterium glutamicum Genome | Applied and Environmental Microbiology
Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... The Corynebacterium glutamicum genome: features and impacts on biotechnological processes. Appl. Microbiol. Biotechnol. 62:99- ...
Frontiers | Efficient Production of the Dicarboxylic Acid Glutarate by Corynebacterium glutamicum via a Novel Synthetic Pathway...
Here, a synthetic pathway for fermentative production of glutarate by the actinobacterium Corynebacterium glutamicum has been ... glutamicum or from three Pseudomonas species. Loss of carbon via formation of the by-products cadaverine and N-acetylcadaverine ... glutamicum or from three Pseudomonas species. Loss of carbon via formation of the by-products cadaverine and N-acetylcadaverine ... Here, a synthetic pathway for fermentative production of glutarate by the actinobacterium Corynebacterium glutamicum has been ...
African Journal of Biotechnology - metabolic engineering of corynebacterium glutamicum to enhance l-leucine production:...
Key words: Corynebacterium glutamicum, L-leucine, metabolic engineering, fermentation, industrial production. ... A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase ( ... IPMS) that carries three amino acid exchanges (R529H, G532D and L535V) from the mutant strain C. glutamicum ML1-9 which was ... This work aimed to develop an efficient L-leucine industrial production strain of Corynebacterium glutamicum by using metabolic ...
ATCCStrainOrganismBiotechnologyStrainsGenesMicrobiologyBacteriumFermentationAminoEncodesPathwayHandbook of CorynebacteriumMetabolismLysineEngineering CorynebacteriumRecombinant CorynebacteriumBacteriaBiotechnologicalPROTEINSGlucoseMetabolically engineered Corynebacterium glutamicumBiomassMetabolic engineering of corynebacteriuAnalysis of the Corynebacterium glutamicumPromoters in Corynebacterium glutamicumEscherichiaCarbonProductionPromoterGene expressionTranscriptional regulatorPhosphoenolpyruvateUptakeSynthaseOxygen deprivationKinoshita2018Genome SequenceRegulationBiologyActinobacteriumMass SpectrometryOverproductionRegulatorSecretionTranscriptomeMicrobial cell factorySuccinateSystems Metabolic EngineeringDeletion
ATCC19
- Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. (wikipedia.org)
- Corynebacterium glutamicum ATCC 14067 was previously known as Brevibacterium flavum. (wikipedia.org)
- Anthocyanidin synthase (ANS) from Petunia hybrida and 3- O -glucosyltransferase (3GT) from Arabidopsis thaliana were coexpressed in C. glutamicum ATCC 13032 to drive the conversion from catechin to C3G. (springer.com)
- This genome is taken from the wild-type strain C. glutamicum ATCC 13032. (kenyon.edu)
- Baumgart M, Unthan S, Rückert C, Sivalingam J, Grünberger A, Kalinowski J, Bott M, Noack S, Frunzke J (2013) Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 for use as a platform strain for basic research and industrial biotechnology. (springer.com)
- Corynebacterium glutamicum ATCC 13032 DNA, complete genome. (atcc.org)
- X78491 C.glutamicum (ATCC 13032) aceB gene. (atcc.org)
- The central carbon metabolism of C. glutamicum has been characterized by enzymatic studies, carbon flux analysis, genetic analysis, and, since the complete genome sequence of ATCC 13032 is available ( 25 ), also by genome-wide studies (reviewed in references 12 , 13 , 59 , and 73 ). (asm.org)
- The procedure has been used successfully for isolation of different medium-copy-number plasmids carrying pHM1519 or pBL1 origins of replication from Corynebacterium glutamicum ATCC 13032. (qiagen.com)
- The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules. (sigmaaldrich.com)
- Regulation of sulphur metabolism in Corynebacterium glutamicum ATCC 13032 has been studied intensively in the last few years, due to its industrial as well as scientific importance. (sigmaaldrich.com)
- A collection of 127 DNA-binding transcriptional regulators was identified in the C. glutamicum ATCC 13032 genome, whereas 103 regulators were detected in C. efficiens YS-314, 63 in C. diphtheriae NCTC 13129 and 55 in C. jeikeium K411. (biomedcentral.com)
- C. glutamicum ATCC 13032 has seven putative sigma factor-encoding genes, including sigA and sigB . (biomedcentral.com)
- Frunzke, Julia 2017-12-01 00:00:00 In this work, we performed a comparative adaptive laboratory evolution experiment of the important biotechnological platform strain Corynebacterium glutamicum ATCC 13032 and its prophage-free variant MB001 towards improved growth rates on glucose minimal medium. (deepdyve.com)
- Corynebacterium glutamicum ATCC 21850 that could produce 162.98 mg L −1 tryptophan was employed as a novel host for metabolic engineering chassis. (biomedcentral.com)
- C. glutamicum strain ATCC 21850 is an established industrial l -tryptophan hyperproducer, which made it an attractive platform since l -tryptophan is direct precursor for the production of crude violacein. (biomedcentral.com)
- Various metabolic engineering and fermentation engineering strategies were applied to C. glutamicum ATCC 21850, and the reported highest violacein titer and productivity were achieved in this work. (biomedcentral.com)
- A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. (uct.ac.za)
- The biocatalytic efficiency of recombinant Corynebacterium glutamicum ATCC 13032 expressing the secondary alcohol dehydrogenase of Micrococcus luteus NCTC2665 was studied. (kaist.ac.kr)
Strain17
- In the present study, a GRAS bacterium, Corynebacterium glutamicum , was introduced as the host strain to synthesize C3G. (springer.com)
- In this study, we used a PDHC-deficient strain of Corynebacterium glutamicum. (asm.org)
- Binder S, Siedler S, Marienhagen J, Bott M, Eggeling L (2013) Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation. (springer.com)
- High productivity and high titers can be achieved in the production process by using the genetically engineered strain of C. glutamicum under the given conditions. (mdpi.com)
- This work aimed to develop an efficient L-leucine industrial production strain of Corynebacterium glutamicum by using metabolic engineering. (academicjournals.org)
- A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase (IPMS) that carries three amino acid exchanges (R529H, G532D and L535V) from the mutant strain C. glutamicum ML1-9 which was obtained by screening for structural analogues. (academicjournals.org)
- By using the genome information of C. glutamicum , we recently found many strain-specific regions existing as "islands" in the common backbone ( 24 ). (asm.org)
- These strain-specific islands (SSIs) were possibly shaped on the genome of the ancestral common strain of two C. glutamicum strains by the integration and deletion of many genes. (asm.org)
- We chose to delete each of 11 SSIs that are larger than 10 kb among thousands of C. glutamicum R strain-specific islands. (asm.org)
- Using a genome-streamlined L -lysine producing strain as basis, metabolic engineering was performed to enable conversion of L -lysine to glutarate in a five-step synthetic pathway comprising lysine decarboxylase, putrescine transaminase and γ-aminobutyraldehyde dehydrogenase from Escherichia coli and GABA/5AVA amino transferase and succinate/glutarate semialdehyde dehydrogenase either from C. glutamicum or from three Pseudomonas species. (frontiersin.org)
- The aim of the present work was rational strain engineering of lysine production by C. glutamicum using a systems-oriented approach. (cuvillier.de)
- Here, we used a Corynebacterium glutamicum strain as a cellular chassis to establish a microbial cell factory for the biotechnological production of ectoines. (biomedcentral.com)
- We further engineered the genome of the basic C. glutamicum strain by integrating a codon-optimized synthetic ectABCD gene cluster under expressional control of the strong and constitutive C. glutamicum tuf promoter. (biomedcentral.com)
- A Corynebacterium glutamicum strain with inactivated pyruvate dehydrogenase complex and a deletion of the gene encoding the pyruvate:quinone oxidoreductase produces about 19 mM L: -valine, 28 mM L: -alanine and about 55 mM pyruvate from 150 mM glucose. (mysciencework.com)
- Promoter activity assays with the malP promoter in C. glutamicum Δ ptsH and WT confirmed that malP transcription is reduced in the PTS-deficient strain. (microbiologyresearch.org)
- As a basis for the construction of a KMV production strain the L-lysine producer C. glutamicum DM1729 was employed. (uni-ulm.de)
- The resulting strain C. glutamicum DM1729 Delta-ilvE (pEK hom FBR thrB thrC) (pMM36ptac ilvA) is the first described strain for the intentional production of KMV from glucose. (uni-ulm.de)
Organism9
- In summary, C. glutamicum proves to be a very energy-efficient organism, which underpins its diverse use as an industrial platform organism for metabolic production. (fz-juelich.de)
- It is not only a workhorse in biotechnology for glutamate and lysine amino acid production, which are used as nutritive additives in food and feed, but also a model organism of systems biology for the investigation of its pathogenic relatives, Corynebacterium diphtheriae , Corynebacterium jeikeium , and mycobacterium [ 1 - 3 ]. (portlandpress.com)
- The gram positive soil bacterium Corynebacterium glutamicum thereby serves as major production organism. (cuvillier.de)
- Corynebacterium glutamicum is most widely known for its role in the industrial production of L-glutamate and L-lysine and as a platform organism for the production of a variety of fine chemicals, biofuels and polymers. (caister.com)
- this in turn makes C. glutamicum the model organism of choice in white biotechnology. (caister.com)
- With a current annual production of more than 2.9 million tons of L-glutamate and more than 1.9 million tons of L-lysine (Ajinomoto, 2013a, b) C. glutamicum already represents a key organism for industrial biotechnology. (caister.com)
- Based on these studies, it becomes increasingly obvious that C. glutamicum has an enormous potential for the biotechnological synthesis of many compounds and materials, making C. glutamicum a versatile platform organism and biotechnology workhorse. (caister.com)
- Consequently, the main objective of this study was to analyse the potential of Corynebacterium glutamicum, which is an important organism in white biotechnology, to produce D-amino acids. (uni-duesseldorf.de)
- C. glutamicum is considered a model organism for other members of the suborder Corynebacterianeae. (biomedcentral.com)
Biotechnology11
- Corynebacterium glutamicum: Biology and Biotechnology. (wikipedia.org)
- Several characteristics of C. glutamicum makes it useful in biotechnology. (kenyon.edu)
- Packed with practical information and state-of-the-art science this concise volume is an essential handbook for everyone working with Corynebacterium and related organisms in academia, biotechnology companies, and the pharmaceutical industry and is a recommended volume for all microbiology libraries. (caister.com)
- Industrial production of amino acid, a key sector of white biotechnology, is the classical field of application of Corynebacterium glutamicum . (caister.com)
- Worth ', ' 825 ': ' San Diego ', ' 800 ': ' Bakersfield ', ' 552 ': ' Presque Isle ', ' 564 ': ' Charleston-Huntington ', ' 528 ': ' Miami-Ft. corynebacterium glutamicum biology and biotechnology articles are allowed on books as of July 2010. (tharge.com)
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- Due to its importance in white biotechnology and in order to ensure more sustainable processes based on non-food renewables and to reduce feedstock costs, C. glutamicum strains have previously been engineered to produce amino acids from GlcNAc. (biomedcentral.com)
- The gram-positive soil bacterium Corynebacterium glutamicum, a major amino acid-producing microorganism in biotechnology, is equipped with several osmoregulated uptake systems for compatible solutes, which is relevant for the physiological response to osmotic stress. (forskningsdatabasen.dk)
Strains5
- Development of fatty acid-producing Corynebacterium glutamicum strains. (uni-bielefeld.de)
- Surprisingly, growth of C. glutamicum strains lacking the general PTS components EI or HPr was strongly impaired on the non-PTS sugar maltose. (microbiologyresearch.org)
- The activities of the maltose transporter and enzymes MalQ, Pgm and GlK were not decreased in PTS-deficient C. glutamicum strains, which was corroborated by comparable transcript amounts of musE , musK and musG , as well as of malQ , in C. glutamicum Δ ptsH and WT. (microbiologyresearch.org)
- Strains of Corynebacterium glutamicum with different lysine productivities may have different lysine excretion systems. (microbiologyresearch.org)
- L-citrulline is an intermediate of L-arginine biosynthesis and accumulates as a by-product of engineered L-arginine producing Corynebacterium glutamicum strains (Ikeda et al. (springeropen.com)
Genes16
- X96580 C.glutamicum panB, panC & xylB genes. (atcc.org)
- Data base searches with the E. coli methionine uptake system MetD as query resulted in the identification of the genes metI, metN and metQ in C. glutamicum. (uni-koeln.de)
- The sigA gene encodes the essential primary sigma factor of C. glutamicum and is responsible for promoter recognition of house-keeping genes. (biomedcentral.com)
- Effect of carbon source availability and growth phase on expression of Corynebacterium glutamicum genes involved in the tricarboxylic acid cycle and glyoxylate bypass. (semanticscholar.org)
- Regulation of the expression of phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) genes in Corynebacterium glutamicum R. (semanticscholar.org)
- The transcriptional regulator SsuR activates expression of the Corynebacterium glutamicum sulphonate utilization genes in the absence of sulphate. (semanticscholar.org)
- Due to the economical coding nature of vio operon, the compressed RBS of vio genes were replaced with complete strong C. glutamicum ones. (biomedcentral.com)
- When the genes crtE, crtB and crtI for conversion of geranylgeranyl pyrophosphate to lycopene were overexpressed in C. glutamicum DeltacrtEb intensely red-pigmented cells and an 80 fold increased lycopene content of 2.4 +/- 0.3 mg/g CDW were obtained. (uni-bielefeld.de)
- Conclusion C. glutamicum possesses a certain degree of redundancy in the biosynthesis of the C50 carotenoid decaprenoxanthin as it possesses two functional phytoene synthase genes. (uni-bielefeld.de)
- Enzymes and genes of the isopropylmalate pathway leading to leucine in Corynebacterium glutamicum were studied, and assays were performed to unravel their connection to lysine oversynthesis. (mysciencework.com)
- In Corynebacterium glutamicum R, the genes ptsH and ptsI encode general components of the PTS, and genes ptsF, ptsS and ptsG each encode fructose-, sucrose- and glucose-specific components of the PTS, respectively. (microbiologyresearch.org)
- Control of the lysine biosynthetic sequence in Corynebacterium glutamicum as analyzed by overexpression of the individual corresponding genes. (microbiologyresearch.org)
- RESULTS The genome sequence of C. glutamicum was searched for genes involved in the assimilatory reduction of inorganic sulphur compounds. (scienceexchange.com)
- A cluster of eight candidate genes could be identified by combining sequence similarity searches with a subsequent synteny analysis between C. glutamicum and the closely related C. efficiens. (scienceexchange.com)
- CONCLUSION The set of C. glutamicum genes involved in assimilatory sulphate reduction was identified and four novel genes involved in this pathway were found. (scienceexchange.com)
- Both genes placed under the control of the C. glutamicum ldhA promoter were expressed at high levels in C. glutamicum, resulting, under oxygen-deprivation conditions, in a significant yield ofethanol from glucose in a process characterized by the absence of cellular growth. (mendeley.com)
Microbiology2
- The Glycosylated Cell Surface Protein Rpf2, Containing a Resuscitation-Promoting Fac- tor Motif, Is Involved in Intercellular Communication of Corynebacterium glutamicum," Archives of Microbiology, Vol. 182, No. 4, 2004, pp. 299-312. (scirp.org)
- SOS-induced spontaneous prophage induction in Corynebacterium glutamicum", Presented at the EMBL Heidelberg "New Approaches and Concepts in Microbiology", Heidelberg, Germany, 2013. (uni-bielefeld.de)
Bacterium12
- Corynebacterium glutamicum (previously known as Micrococcus glutamicus) is a Gram-positive, rod-shaped bacterium that is used industrially for large-scale production of amino acids. (wikipedia.org)
- C. glutamicum is a small, non-moving Gram-positive soil bacterium. (kenyon.edu)
- C. glutamicum is a non-pathogenic bacterium, although a related species, C. diphtheriae is pathogenic and causes diphtheria in humans through a strong exotoxin it produces. (kenyon.edu)
- We developed a model system for the biodegradation of phenol-contaminated wastewater by the bacterium Corynebacterium glutamicum . (scirp.org)
- Corynebacterium glutamicum is a high-G+C content gram-positive soil bacterium which was isolated in 1957 as an l -glutamate-excreting bacterium ( 1 , 32 ). (asm.org)
- Amino acid- producing gram-positive bacterium Corynebacterium glutamicum codes for seven sigma factors: the primary sigma factor SigA, the primary-like sigma factor SigB and five ECF stress- responsive sigma factors (SigC, SigD, SigE, SigH and SigM). (cuni.cz)
- Corynebacterium glutamicum is a high G+C - content aerobic Gram-positive bacterium. (portlandpress.com)
- Corynebacterium glutamicum is a gram-positive soil bacterium widely used for the industrial production of amino acids. (biomedcentral.com)
- C. glutamicum is a Gram-positive biotin-auxotrophic bacterium that was originally isolated as an L-glutamate producer from soil samples [ 8 ]. (biomedcentral.com)
- BACKGROUND Corynebacterium glutamicum is a high-GC Gram-positive soil bacterium of great biotechnological importance for the production of amino acids. (scienceexchange.com)
- Growth experiments revealed that the non-pathogenic soil bacterium C. glutamicum is able to use hemin or hemoglobin as sole iron source. (omicsdi.org)
- The molecular cloning and sequence of the odhA gene encoding 2-oxoglutarate dehydrogenase (E1o subunit of the 2-oxoglutarate dehydrogenase complex) of Corynebacterium glutamicum, a coryneform, Gram-positive bacterium widely used in the industrial production of amino acids, are reported. (readabstracts.com)
Fermentation8
- C. glutamicum breaks down carbohydrates through the process of fermentation. (kenyon.edu)
- Anusree M, Wendisch VF, Nampoothiri KM (2016) Co-expression of endoglucanase and beta-glucosidase in Corynebacterium glutamicum DM1729 towards direct lysine fermentation from cellulose. (springer.com)
- Due to its robustness and well established large-scale fermentation, C. glutamicum has been engineered for the production of a number of value-added compounds including terpenoids. (mdpi.com)
- Thus, the ability of C. glutamicum to combat ROS challenges and maintain the cellular redox balance is crucial for cell survival and improves fermentation efficiency. (portlandpress.com)
- Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium 's GRAS status and advantages in tryptophan fermentation. (biomedcentral.com)
- With metabolic engineering and fermentation optimization practice, C. glutamicum 21850 (pEC-C-vio1) was able to produce violacein with both titer and productivity at the highest level ever reported. (biomedcentral.com)
- Due to advantages of mature C. glutamicum fermentation industry, this work has built basis for commercial production of violacein. (biomedcentral.com)
- (EN) Disclosed is a L-isoleucine-producing Corynebacterium glutamicum fermentation medium, comprising a basal medium and a growth factor, wherein the growth factor consists of choline, betaine and vitamin B6, and the contents of each ingredient in the fermentation medium are: 0.2-1 g/L choline, 0.25-0.5 mg/L betaine, and 0.05-0.3 mg/L vitamin B6. (wipo.int)
Amino14
- While originally identified in a screen for organisms secreting L-glutamate, mutants of C. glutamicum have also been identified that produce various other amino acids. (wikipedia.org)
- Through its metabolism, C. glutamicum synthesizes such products as serine, glutamate, and lysine (amino acids), all of which are used in many different ways, such as in pharmacies. (kenyon.edu)
- This technique could be further developed, as well as transferred to increase production of other C. glutamicum products , such as other amino acids. (kenyon.edu)
- Corynebacterium glutamicum ( C. glutamicum ), an important industrial workhorse, is capable of efficiently producing a variety of value-added chemicals and fuels beyond amino acids. (springer.com)
- Corynebacterium glutamicum is the prominent host for the fermentative production of amino acids with an average annual production volume of ~6 million tons. (mdpi.com)
- Besides by distinct amino acids of AmtR, DNA binding is influenced by nucleotides not only of the conserved binding motif but also by spacing nucleotides in C. glutamicum. (nih.gov)
- Amino sugars such as glucosamine or N-acetylglucosamine are potential alternative carbon sources for biotechnological processes with Corynebacterium glutamicum. (uni-koeln.de)
- Corynebacterium glutamicum is widely used for the industrial production of amino acids, nucleotides, and vitamins. (uio.no)
- Proteomics is an important technique to study the biology of Corynebacterium glutamicum with focus on processes related to the biotechnological production of amino acids and other chemicals. (caister.com)
- The amino acid-producing Gram-positive Corynebacterium glutamicum is auxotrophic for biotin although biotin ring assembly starting from the precursor pimeloyl-CoA is still functional. (biomedcentral.com)
- Corynebacterium glutamicum is an important industrial producer of various amino acids and other metabolites. (springeropen.com)
- Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. (uct.ac.za)
- New trends in Corynebacterium glutamicum: beyond the amino acids , Nova Sciences Pub, 2015, 978-1-63483-280-9. (univ-lorraine.fr)
- Corynebacterium glutamicum ( C. glutamicum ) has traditionally been used as a microbial cell factory for the industrial production of many amino acids and other industrially important co. (biomedcentral.com)
Encodes5
- Although the genome of Corynebacterium glutamicum encodes a large number of the putative MarR-type transcriptional regulators, their physiological and biochemical functions have so far been limited to only two proteins, regulator of oxidative stress response RosR and quinone oxidoreductase regulator QosR. (portlandpress.com)
- However, the genome of C. glutamicum also encodes a second carotenoid gene cluster comprising crtB2I2-1/2 shown to be co-transcribed, as well. (uni-bielefeld.de)
- The C. glutamicum genome encodes seven sigma subunits (factors) of RNA polymerase: the primary sigma factor SigA (σ A ), the primary-like σ B and five alternative sigma factors (σ C , σ D , σ E , σ H and σ M ). We have developed in vitro and in vivo methods to assign particular sigma factors to individual promoters of different classes. (springeropen.com)
- The C. glutamicum genome encodes seven sigma subunits of RNAP: the primary sigma factor σ A , the alternative primary-like σ B and five other alternative σ factors with extracytoplasmic functions (ECF) (σ C , σ D , σ E , σ H and σ M ) (for a review, see Pátek and Nešvera 2011 ). (springeropen.com)
- The pck (cg3169) gene of Corynebacterium glutamicum encodes a phosphoenolpyruvate carboxykinase (PEPCK). (elsevier.com)
Pathway5
- Biosynthetic pathway of anthocyanins (labeled in pink) in plants and the introduced pathway for cyanidin 3- O -glucoside production from catechin in C. glutamicum . (springer.com)
- The introduced pathway consisting of ANS and 3GT is highlighted in red, and the biosynthetic pathway of UDP-glucose in C. glutamicum is marked in green. (springer.com)
- Here, a synthetic pathway for fermentative production of glutarate by the actinobacterium Corynebacterium glutamicum has been developed. (frontiersin.org)
- The fruits of molecular physiology: engineering the l-isoleucine biosynthesis pathway in Corynebacterium glutamicum. (academicjournals.org)
- The central metabolic pathway of Corynebacterium glutamicum was engineered to produce ethanol. (mendeley.com)
Handbook of Corynebacterium1
- Handbook of Corynebacterium glutamicum. (academicjournals.org)
Metabolism10
- Newly added topics include cell envelope structures and aromatic compound metabolism in C. glutamicum. (springer.com)
- Due to the variance in the availability of nutrients and carbon sources, C. glutamicum has 127 proteins associated with a regulatory function in transcription, which in turn control metabolism. (kenyon.edu)
- The substrate spectrum of C. glutamicum has been further extended by detailed knowledge of carbon core metabolism and well-established genetic tools and engineering strategies. (springer.com)
- Acetate metabolism and its regulation in Corynebacterium glutamicum. (academicjournals.org)
- Previously, the gene cg0156 was shown to belong to the regulon of McbR, a global transcriptional repressor of sulphur metabolism in C. glutamicum. (sigmaaldrich.com)
- Therefore, it is an interesting candidate for study to further the understanding of the regulation of sulphur metabolism in C. glutamicum. (sigmaaldrich.com)
- Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR. (semanticscholar.org)
- Complete sucrose metabolism requires fructose phosphotransferase activity in Corynebacterium glutamicum to ensure phosphorylation of liberated fructose. (microbiologyresearch.org)
- Complementation experiments showed that a functional PTS phosphorelay is required for optimal growth of C. glutamicum on maltose, implying its involvement in the control of maltose metabolism and/or uptake. (microbiologyresearch.org)
- This monograph illustrates how the data gleaned from whole genome sequencing permits the rational engineering of the total mobile metabolism and the way platforms biology allows the additional optimization of C. glutamicum as a biocatalyst. (globalinvests.com)
Lysine9
- Broer S, Kramer R. Lysine uptake and exchange in Corynebacterium glutamicum. (atcc.org)
- From zero to hero--design-based systems metabolic engineering of Corynebacterium glutamicum for L-lysine production. (academicjournals.org)
- Deregulation of feedback inhibition of phosphoenolpyruvate carboxylase for improved lysine production in Corynebacterium glutamicum. (academicjournals.org)
- Given its unique ability to co-utilize mixed carbon sources, C. glutamicum could be used to clean-up of wastes from agricultural or other industries, simultaneously producing useful compounds such as L-lysine or putrescine. (caister.com)
- 1) The addition of 100 mM D-threonine, D-lysine, D-arginine, D-alanine, D-serine, D-asparagine or D-methionine led to only a slight reduction in the growth rate of C. glutamicum. (uni-duesseldorf.de)
- Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity. (semanticscholar.org)
- Leucine synthesis in Corynebacterium glutamicum: enzyme activities, structure of leuA, and effect of leuA inactivation on lysine synthesis. (mysciencework.com)
- Fermentative Production of Lysine by Corynebacterium glutamicum from Different Carbon Sources', Iran Agricultural Research , 25.26(1.2), pp. 99-106. (ac.ir)
- Production of lysine by Corynebacterium glutamicum (PTCC 1532) from different agricultural by-products (molasses and pulpy waste date) was compared to glucose as raw materials. (ac.ir)
Engineering Corynebacterium2
- Engineering Corynebacterium glutamicum for the production of p. (mysciencework.com)
- Engineering Corynebacterium glutamicum for the production of pyruvate. (mysciencework.com)
Recombinant Corynebacterium2
- Jojima T, Noburyu R, Suda M, Okino S, Yukawa H, Inui M. Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant Corynebacterium glutamicum . (mdpi.com)
- This study constitutes the first report of significant production of 12-ketooleic acid using a recombinant Corynebacterium glutamicum-based biocatalyst. (kaist.ac.kr)
Bacteria3
- Many bacteria can synthesize biotin, but biotin auxotrophic bacteria such as Corynebacterium glutamicum require uptake of biotin from the habitat. (biomedcentral.com)
- The suborder Corynebacterianeae comprises bacteria like Mycobacterium tuberculosis and Corynebacterium glutamicum, and these bacteria contain in addition to the linear fatty acids, unique α-branched β-hydroxy fatty acids, called mycolic acids. (northumbria.ac.uk)
- Corynebacterium efficiens is a thermotolerant, glutamic acid-producing (from dextrin) species of bacteria from soil and vegetables. (wikipedia.org)
Biotechnological3
- This book provides a comprehensive overview of current knowledge and research on C. glutamicum systems biology and biotechnological applications. (caister.com)
- We successfully applied metabolic engineering strategies to optimize its synthetic production in the industrial workhorse C. glutamicum and thereby paved the way for further improvements in ectoine yield and biotechnological process optimization. (biomedcentral.com)
- The current work deals with the biotechnological synthesis of alpha-ketomethylvalerate (KMV) with Corynebacterium glutamicum. (uni-ulm.de)
PROTEINS4
- Technologies for quantification, membrane enrichment and MS analysis tested in C. glutamicum might lead to higher coverage for membrane proteins or improve the accuracy and reliability of quantification based on isotope labeling. (caister.com)
- We analyzed the function of three Acr3 proteins from Corynebacterium glutamicum, CgAcr3-1, CgAcr3-2, and CgAcr3-3. (ac.be)
- N Engl J Med 298:27-29 J€ager W, Peters-Wendisch PG, Kalinowski J, P€ uhler A (1996) A Corynebacterium glutamicum gene encoding a two-domain protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins. (globalinvests.com)
- With the proposed project we aim to investigate the impact of stress responses and stochasticity fluctuations of key regulatory proteins on the spontaneous induction of the Corynebacterium glutamicum prophage CGP3 at the single cell level. (tu-bs.de)
Glucose10
- Thus, while during growth on gluconeogenic carbon sources SugR represses ptsG , ptsG expression is derepressed during growth on glucose or under other conditions characterized by high fructose-6-phosphate concentrations, representing one mechanism which allows C. glutamicum to adapt glucose uptake to carbon source availability. (asm.org)
- The glucose uptake in C. glutamicum appears to be regulated, as, e.g., during growth on glucose-acetate mixtures glucose uptake is reduced about twofold in comparison to growth on glucose alone ( 71 ). (asm.org)
- Aerobically cultivated cells of Corynebacterium glutamicum produce mixed organic acids, including succinic acid (SA), from glucose when the cells are transferred to oxygen-deprived conditions. (mdpi.com)
- Co-ordinated regulation of gluconate catabolism and glucose uptake in Corynebacterium glutamicum by two functionally equivalent transcriptional regulators, GntR1 and GntR2. (academicjournals.org)
- In a comparative approach, C. glutamicum cells were grown with either glucose or acetate as the sole carbon source prior to immunoprecipitation. (epfl.ch)
- After mapping of these data on the genome sequence of C. glutamicum, 107 enriched DNA fragments were detected from cells grown with glucose as carbon source. (epfl.ch)
- Overall, this study resulted in the identification of causative key mutations improving the growth of C. glutamicum on glucose. (deepdyve.com)
- Based on this double mutant C. glutamicum △aceE △pqo, we engineered C. glutamicum for efficient production of pyruvate from glucose by additional deletion of the ldhA gene encoding NAD(+)-dependent L: -lactate dehydrogenase (LdhA) and introduction of a attenuated variant of the acetohydroxyacid synthase (△C-T IlvN). (mysciencework.com)
- The Gram-positive Corynebacterium glutamicum co-metabolizes most carbon sources such as the phosphotransferase system (PTS) sugar glucose and the non-PTS sugar maltose. (microbiologyresearch.org)
- Based on these results, PckR seemed to play an important role in the regulation of PEPCK in C. glutamicum grown in glucose. (elsevier.com)
Metabolically engineered Corynebacterium glutamicum1
- Production and glucosylation of C50 and C 40 carotenoids by metabolically engineered Corynebacterium glutamicum. (uni-bielefeld.de)
Biomass3
- Elisabeth Zelle, Nina Pfelzer, Marco Oldiges, Abigail Koch-Koerfges, Michael Bott, Katharina Nöh, Wolfgang Wiechert: An energetic profile of Corynebacterium glutamicum underpinned by measured biomass yield on ATP. (fz-juelich.de)
- Corynebacterium glutamicum was metabolically engineered to produce D-lactate from biomass. (asu.edu)
- C. glutamicum was cultured with aromatics, such as vanillin, vanillic acid, and coumarate that are present in biomass hydrolysates and will be analyzed for the presence of D-lactate. (asu.edu)
Metabolic engineering of corynebacteriu2
- Systems Metabolic Engineering of Corynebacterium glutamicum for Biobased Production of Chemicals, Materials and Fuels. (academicjournals.org)
- CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum. (semanticscholar.org)
Analysis of the Corynebacterium glutamicum2
- Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase. (microbiologyresearch.org)
- Functional genomics and expression analysis of the Corynebacterium glutamicum fpr2-cysIXHDNYZ gene cluster involved in assimilatory sulphate reduction. (scienceexchange.com)
Promoters in Corynebacterium glutamicum1
- Sigma factors and promoters in Corynebacterium glutamicum. (semanticscholar.org)
Escherichia2
- In contrast to Escherichia coli and Bacillus subtilis , which show distinct catabolite repression, C. glutamicum usually coutilizes the carbon sources present in the mixtures without showing diauxic growth. (asm.org)
- CgAcr3-1 was also the most active when everted membranes vesicles from Escherichia coli or C. glutamicum mutants were assayed for efflux with different energy sources. (ac.be)
Carbon3
- C. glutamicum has a broad natural substrate spectrum and can simultaneously utilize various carbon sources in blends. (springer.com)
- A key development in recent years has been the engineering of C. glutamicum to utilize a broader spectrum of carbon sources (e.g. glycerol, galactose and pentose sugars) in addition to the traditional hexoses. (caister.com)
- Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. (microbiologyresearch.org)
Production13
- For the first time, we successfully achieved the production of the specialty anthocyanin C3G from the comparatively inexpensive flavonoid precursor catechin in C. glutamicum . (springer.com)
- Another area of research into C. glutamicum is how to increase its production of L-glutamic acid, which is produced annually at a rate of about 1.5 million tons. (kenyon.edu)
- Becker J, Wittmann C (2012) Bio-based production of chemicals, materials and fuels- Corynebacterium glutamicum as versatile cell factory. (springer.com)
- The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. (asm.org)
- Bio-based production of chemicals, materials and fuels-Corynebacterium glutamicum as versatile cell factory. (academicjournals.org)
- Corynebacterium glutamicum tailored for high-yield L-valine production. (academicjournals.org)
- C. glutamicum cells are frequently confronted with excessive reactive oxygen species (ROS) production, triggering sudden changes of fermentative conditions in temperature, pH, osmotic pressure, or toxic compounds [ 4 - 5 ]. (portlandpress.com)
- 2007). Furthermore, within the last decade, C. glutamicum was applied in various production processes for fine chemicals, fuels and polymers. (caister.com)
- Already metabolic engineering of only the terminal reactions leading to lycopene resulted in considerable lycopene production indicating that C. glutamicum may serve as a potential host for carotenoid production. (uni-bielefeld.de)
- Biotin auxotrophy of C. glutamicum elicits L-glutamate production, a characteristic which led to its discovery. (biomedcentral.com)
- A succinate efflux pump, SucE, was recently identified in C. glutamicum , which substantially increases succinate production when overexpressed. (shef.ac.uk)
- L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicum . (springeropen.com)
- To enrich the knowledge of metabolic sub-network of L-valine syntheses for higher production of L-valine , Corynebacterium glutamicum AS1.495 and its genetic derivatives AA361, AAT231, AATV341 were used for metabolic flux analysis . (bvsalud.org)
Promoter2
- To extend its product spectrum and improve productivity, C. glutamicum needs to undergo further engineering, including the development of applicable promoter system. (semanticscholar.org)
- Here, we isolated new promoters from the fully synthetic promoter library consisting of 70-bp random sequences in C. glutamicum. (semanticscholar.org)
Gene expression3
- Abigail Koch-Koerfges, Armin Kabus, Ines Ochrombel, Kay Marin, Michael Bott: Physiology and global gene expression of a Corynebacterium glutamicum ΔF(1)F(O)-ATP synthase mutant devoid of oxidative phosphorylation. (fz-juelich.de)
- Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum. (semanticscholar.org)
- Since most examples of regulation of gene expression in C . glutamicum describe transcriptional control, the main focus of this chapter is this level of regulation. (routledgehandbooks.com)
Transcriptional regulator2
- Here, we report that the ncgl2617 gene ( cosR ) of C. glutamicum encoding an MarR-type transcriptional regulator plays an important role in oxidative stress resistance. (portlandpress.com)
- The transcriptional regulator GlxR has been characterized as a global hub within the gene-regulatory network of Corynebacterium glutamicum. (epfl.ch)
Phosphoenolpyruvate1
- Mori and Shiio ( 43 ) first mentioned the presence of a phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) in C. glutamicum . (asm.org)
Uptake4
- The characterization of methionine uptake in C. glutamicum revealed two uptake systems. (uni-koeln.de)
- Comparative genome analyses suggested that the putative transport system BioYMN encoded by cg2147, cg2148 and cg2149 might be involved in biotin uptake by C. glutamicum . (biomedcentral.com)
- C. glutamicum lacks an uptake system for the peptidoglycan constituent N-acetylglucosamine (GlcNAc), but is able to catabolize GlcNAc-6-phosphate. (biomedcentral.com)
- tion systems of C. glutamicum impede its uptake of foreign DNA and thus limiting its transformation efficiency. (globalspec.com)
Synthase1
- Ectopic expression of crtB2 could compensate for the lack of phytoene synthase CrtB in C. glutamicum DeltacrtB, thus, C. glutamicum possesses two functional phytoene synthases, namely CrtB and CrtB2. (uni-bielefeld.de)
Oxygen deprivation3
- The established bioprocess is an elegant approach for the systematic understanding of C. glutamicum 's adaptation to a progressive oxygen deprivation. (aiche.org)
- Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions. (microbiologyresearch.org)
- Since the reaction occurred in the absence of cell growth, the ethanol volumetric productivity increased in proportion to cell density of ethanologenic C. glutamicum in a process under oxygen-deprivation conditions. (mendeley.com)
Kinoshita1
- Corynebacterium glutamicum (Kinoshita et al. (atcc.org)
20181
- Becker J, Giesselmann G, Hoffmann SL, Wittmann C (2018) Corynebacterium glutamicum for sustainable bioproduction: from metabolic physiology to systems metabolic engineering. (springer.com)
Genome Sequence1
- Genome sequence for C. glutamicum from NCBI. (wikipedia.org)
Regulation1
- The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. (uct.ac.za)
Biology7
- This updated second edition covers the molecular biology, genome engineering tools and comprehensive analysis techniques for Corynebacterium glutamicum . (springer.com)
- In summary, proteomics data are important for systems biology approaches in C. glutamicum . (caister.com)
- A trusted corynebacterium glutamicum biology and is Participation Teachers science innovation in Domain Insights. (tharge.com)
- We are also exploring your corynebacterium glutamicum biology and. (tharge.com)
- 3 right of 5 corynebacterium glutamicum biology anything stock severe use ET( slim new millennium( use your statements with bloodthirsty class a process Edition all 7 message t board version sent a d draining digits somehow manually. (tharge.com)
- You can have about corynebacterium glutamicum biology job in the Spanish4Teachers TechBlog. (tharge.com)
- The group of YANG Sheng at CAS SIBS Key Laboratory of Synthetic Biology developed a a Francisella novicida CRISPR-Cpf1-based genome-editing method for C. glutamicum. (window-to-china.eu)
Actinobacterium1
- Here, we investigate the role of DNA-partitioning protein ParB and SMC condensin complexes in the actinobacterium Corynebacterium glutamicum . (nature.com)
Mass Spectrometry3
- For this purpose, the glycerophospholipidome of C. glutamicum grown at different temperatures was analyzed by mass spectrometry using quantitative multiple precursor ion scanning. (forskningsdatabasen.dk)
- We have confirmed by mass spectrometry the absence of poly-β-hydrobutyrate (PHB) in C. glutamicum. (kribb.re.kr)
- Recombinant C. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). (kaist.ac.kr)
Overproduction1
- In this study, C. glutamicum was engineered for overproduction of L-citrulline as major product without L-arginine being produced as by-product. (springeropen.com)
Regulator4
- The TetR family member AmtR is the central regulator of nitrogen starvation response in Corynebacterium glutamicum. (nih.gov)
- Together, the results reveal that CosR is a redox-sensitive regulator that senses peroxide stress to mediate oxidative stress resistance in C. glutamicum . (portlandpress.com)
- The response regulator HrrA belonging to the HrrSA two-component system (previously named CgtSR11) is known to be repressed by the global iron-dependent regulator DtxR in Corynebacterium glutamicum. (omicsdi.org)
- As a central part of this study, we investigated the regulon of the response regulator HrrA via comparative transcriptome analysis of a hrrA deletion mutant and C. glutamicum wild type in combination with DNA-protein interaction studies with purified HrrA protein. (omicsdi.org)
Secretion1
- Heterologous expression of exochitinase gene chiB from Serratia marcescens resulted in chitinolytic activity and ChiB secretion was enhanced when a signal peptide from C. glutamicum was used. (biomedcentral.com)
Transcriptome1
- This database contains 8772 oligonucleotides to represent the Corynebacterium glutamicum R transcriptome. (umich.edu)
Microbial cell factory2
- At present, many pathways have been successfully engineered in C. glutamicum for access to alternative renewable sources to produce natural or non-natural products, making C. glutamicum a promising and favorable microbial cell factory. (springer.com)
- Compared to E. coli, C. glutamicum is generally recognized as safes (GRAS) and exhibits numerous advantages as a microbial cell factory. (biomedcentral.com)
Succinate1
- A comprehensive understanding of SucE's transport mechanism could allow us to manipulate this transporter and/or it's energy source to make succinate (and possibly other dicarboxylic acids) efflux more efficient, potentially increasing the succinate yield of C. glutamicum . (shef.ac.uk)
Systems Metabolic Engineering1
- Corynebacterium glutamicum for Sustainable Bioproduction: From Metabolic Physiology to Systems Metabolic Engineering. (academicjournals.org)
Deletion1
- Two-component systems of Corynebacterium glutamicum: deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response. (semanticscholar.org)