Corynebacterium glutamicum: A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.Corynebacterium: A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.Corynebacterium Infections: Infections with bacteria of the genus CORYNEBACTERIUM.Corynebacterium diphtheriae: A species of gram-positive, asporogenous bacteria in which three cultural types are recognized. These types (gravis, intermedius, and mitis) were originally given in accordance with the clinical severity of the cases from which the different strains were most frequently isolated. This species is the causative agent of DIPHTHERIA.Bacterial Proteins: Proteins found in any species of bacterium.Corynebacterium pseudotuberculosis: A species of gram-positive, asporogenous bacteria that was originally isolated from necrotic areas in the kidney of a sheep. It may cause ulcerative lymphangitis, abscesses, and other chronic purulent infections in sheep, horses, and other warm-blooded animals. Human disease may form from contact with infected animals.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Metabolic Engineering: Methods and techniques used to genetically modify cells' biosynthetic product output and develop conditions for growing the cells as BIOREACTORS.Propionibacterium acnes: A bacteria isolated from normal skin, intestinal contents, wounds, blood, pus, and soft tissue abscesses. It is a common contaminant of clinical specimens, presumably from the skin of patients or attendants.Genes, Bacterial: The functional hereditary units of BACTERIA.Gene Deletion: A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.Mycolic AcidsMolecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Brevibacterium: A gram-positive organism found in dairy products, fresh and salt water, marine organisms, insects, and decaying organic matter.Oxaloacetic Acid: A dicarboxylic acid ketone that is an important metabolic intermediate of the CITRIC ACID CYCLE. It can be converted to ASPARTIC ACID by ASPARTATE TRANSAMINASE.Biotechnology: Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.Betaine: A naturally occurring compound that has been of interest for its role in osmoregulation. As a drug, betaine hydrochloride has been used as a source of hydrochloric acid in the treatment of hypochlorhydria. Betaine has also been used in the treatment of liver disorders, for hyperkalemia, for homocystinuria, and for gastrointestinal disturbances. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1341)Operon: In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.Lysine: An essential amino acid. It is often added to animal feed.Industrial Microbiology: The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.Succinic Acid: A water-soluble, colorless crystal with an acid taste that is used as a chemical intermediate, in medicine, the manufacture of lacquers, and to make perfume esters. It is also used in foods as a sequestrant, buffer, and a neutralizing agent. (Hawley's Condensed Chemical Dictionary, 12th ed, p1099; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1851)Diphtheria: A localized infection of mucous membranes or skin caused by toxigenic strains of CORYNEBACTERIUM DIPHTHERIAE. It is characterized by the presence of a pseudomembrane at the site of infection. DIPHTHERIA TOXIN, produced by C. diphtheriae, can cause myocarditis, polyneuritis, and other systemic toxic effects.Corynebacterium pyogenes: A species of CORYNEBACTERIUM isolated from abscesses of warm-blooded animals.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Gentisates: Salts and esters of gentisic acid.Amino Acids, DiaminoEscherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Biosynthetic Pathways: Sets of enzymatic reactions occurring in organisms and that form biochemicals by making new covalent bonds.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Homoserine Dehydrogenase: An enzyme that catalyzes the reduction of aspartic beta-semialdehyde to homoserine, which is the branch point in biosynthesis of methionine, lysine, threonine and leucine from aspartic acid. EC 1.1.1.3.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Hydroxybenzoates: Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.Pentose Phosphate Pathway: An oxidative decarboxylation process that converts GLUCOSE-6-PHOSPHATE to D-ribose-5-phosphate via 6-phosphogluconate. The pentose product is used in the biosynthesis of NUCLEIC ACIDS. The generated energy is stored in the form of NADPH. This pathway is prominent in tissues which are active in the synthesis of FATTY ACIDS and STEROIDS.Metabolic Networks and Pathways: Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.Genetic Enhancement: The use of genetic methodologies to improve functional capacities of an organism rather than to treat disease.Prephenate Dehydratase: An enzyme that catalyzes the conversion of prephenate to phenylpyruvate with the elimination of water and carbon dioxide. In the enteric bacteria this enzyme also possesses chorismate mutase activity, thereby catalyzing the first two steps in the biosynthesis of phenylalanine. EC 4.2.1.51.Ketol-Acid Reductoisomerase: An enzyme that catalyzes the oxidation of (R)-2,3-dihydroxy-3-methylbutanoate to (S)-2-hydroxy-2-methyl-3-oxobutanoate in the presence of NADP. It is involved in the biosynthesis of VALINE; LEUCINE; ISOLEUCINE; pentothenate and COENZYME A. This enzyme was formerly classified as EC 1.1.1.89.Pimelic Acids: A group of compounds that are derivatives of heptanedioic acid with the general formula R-C7H11O4.Galactans: Polysaccharides composed of repeating galactose units. They can consist of branched or unbranched chains in any linkages.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Isoleucine: An essential branched-chain aliphatic amino acid found in many proteins. It is an isomer of LEUCINE. It is important in hemoglobin synthesis and regulation of blood sugar and energy levels.Electrophoretic Mobility Shift Assay: An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.Malate Synthase: An important enzyme in the glyoxylic acid cycle which reversibly catalyzes the synthesis of L-malate from acetyl-CoA and glyoxylate. This enzyme was formerly listed as EC 4.1.3.2.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Artificial Gene Fusion: The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.Valine: A branched-chain essential amino acid that has stimulant activity. It promotes muscle growth and tissue repair. It is a precursor in the penicillin biosynthetic pathway.Glutamic Acid: A non-essential amino acid naturally occurring in the L-form. Glutamic acid is the most common excitatory neurotransmitter in the CENTRAL NERVOUS SYSTEM.Regulon: In eukaryotes, a genetic unit consisting of a noncontiguous group of genes under the control of a single regulator gene. In bacteria, regulons are global regulatory systems involved in the interplay of pleiotropic regulatory domains and consist of several OPERONS.Chorismate Mutase: An isomerase that catalyzes the conversion of chorismic acid to prephenic acid. EC 5.4.99.5.Membrane Transport Proteins: Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.Glyceraldehyde 3-Phosphate: An aldotriose which is an important intermediate in glycolysis and in tryptophan biosynthesis.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Methylophilus methylotrophus: A species of METHYLOPHILUS which is motile by single flagella. In addition to growth on methanol as a sole carbon source, growth also occurs on glucose. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Isocitrate Lyase: A key enzyme in the glyoxylate cycle. It catalyzes the conversion of isocitrate to succinate and glyoxylate. EC 4.1.3.1.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Diphtheria Toxin: An ADP-ribosylating polypeptide produced by CORYNEBACTERIUM DIPHTHERIAE that causes the signs and symptoms of DIPHTHERIA. It can be broken into two unequal domains: the smaller, catalytic A domain is the lethal moiety and contains MONO(ADP-RIBOSE) TRANSFERASES which transfers ADP RIBOSE to PEPTIDE ELONGATION FACTOR 2 thereby inhibiting protein synthesis; and the larger B domain that is needed for entry into cells.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Multigene Family: A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)Phosphoenolpyruvate Sugar Phosphotransferase System: The bacterial sugar phosphotransferase system (PTS) that catalyzes the transfer of the phosphoryl group from phosphoenolpyruvate to its sugar substrates (the PTS sugars) concomitant with the translocation of these sugars across the bacterial membrane. The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. The PTS has also been implicated in the induction of synthesis of some catabolic enzyme systems required for the utilization of sugars that are not substrates of the PTS as well as the regulation of the activity of ADENYLYL CYCLASES. EC 2.7.1.-.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Pyruvic Acid: An intermediate compound in the metabolism of carbohydrates, proteins, and fats. In thiamine deficiency, its oxidation is retarded and it accumulates in the tissues, especially in nervous structures. (From Stedman, 26th ed)Phosphate Acetyltransferase: An enzyme that catalyzes the synthesis of acetylphosphate from acetyl-CoA and inorganic phosphate. Acetylphosphate serves as a high-energy phosphate compound. EC 2.3.1.8.GlyoxylatesRepressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Acetolactate Synthase: A flavoprotein enzyme that catalyzes the formation of acetolactate from 2 moles of PYRUVATE in the biosynthesis of VALINE and the formation of acetohydroxybutyrate from pyruvate and alpha-ketobutyrate in the biosynthesis of ISOLEUCINE. This enzyme was formerly listed as EC 4.1.3.18.Mannosyltransferases: Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC 2.4.1.32, EC 2.4.1.48, EC 2.4.1.54, and EC 2.4.1.57.

Evolutionary process of amino acid biosynthesis in Corynebacterium at the whole genome level. (1/403)

Corynebacterium glutamicum, which is the closest relative of Corynebacterium efficiens, is widely used for the large scale production of many kinds of amino acids, particularly glutamic acid and lysine, by fermentation. Corynebacterium diphtheriae, which is well known as a human pathogen, is also closely related to these two species of Corynebacteria, but it lacks such productivity of amino acids. It is an important and interesting question to ask how those closely related bacterial species have undergone such significant functional differentiation in amino acid biosynthesis. The main purpose of the present study is to clarify the evolutionary process of functional differentiation among the three species of Corynebacteria by conducting a comparative analysis of genome sequences. When Mycobacterium and Streptomyces were used as out groups, our comparative study suggested that the common ancestor of Corynebacteria already possessed almost all of the gene sets necessary for amino acid production. However, C. diphtheriae was found to have lost the genes responsible for amino acid production. Moreover, we found that the common ancestor of C. efficiens and C. glutamicum have acquired some of genes responsible for amino acid production by horizontal gene transfer. Thus, we conclude that the evolutionary events of gene loss and horizontal gene transfer must have been responsible for functional differentiation in amino acid biosynthesis of the three species of Corynebacteria.  (+info)

Purification and characterization of O-Acetylserine sulfhydrylase of Corynebacterium glutamicum. (2/403)

We highly purified O-acetylserine sulfhydrylase from the glutamate-producing bacterium Corynebacterium glutamicum. The molecular mass of the purified enzyme was 34,500 as determined by SDS-polyacrylamide gel electrophoresis, and 70,800 as determined by gel filtration chromatography. It had an apparent Km of 7.0 mM for O-acetylserine and a Vmax of 435 micromol min-1 (mg x protein)-1. This is the first report of the cysteine biosynthetic enzyme of C. glutamicum in purified form.  (+info)

BetP of Corynebacterium glutamicum, a transporter with three different functions: betaine transport, osmosensing, and osmoregulation. (3/403)

In order to circumvent deleterious effects of hypo- and hyperosmotic conditions in its environment, Corynebacterium glutamicum has developed a number of mechanisms to counteract osmotic stress. The first response to an osmotic upshift is the activation of uptake mechanisms for the compatible solutes betaine, proline, or ectoine, namely BetP, EctP, ProP, LcoP and PutP. BetP, the most important uptake system responds to osmotic stress by regulation at the level of both protein activity and gene expression. BetP was shown to harbor three different properties, i.e. catalytic activity (betaine transport), sensing of appropriate stimuli (osmosensing) and signal transduction to the catalytic part of the carrier protein which adapts its activity to the extent of osmotic stress (osmoregulation). BetP is comprised of 12 transmembrane segments and carries N- and C-terminal domains, which are involved in osmosensing and/or osmoregulation. Recent results on molecular properties of these domains indicate the significance of particular amino acids within the terminal 25 amino acids of the C-terminal domain of BetP for the process of osmosensing and osmoregulation.  (+info)

Acyl-CoA carboxylases (accD2 and accD3), together with a unique polyketide synthase (Cg-pks), are key to mycolic acid biosynthesis in Corynebacterianeae such as Corynebacterium glutamicum and Mycobacterium tuberculosis. (4/403)

The Corynebacterianeae such as Corynebacterium glutamicum and Mycobacterium tuberculosis possess several unique and structurally diverse lipids, including the genus-specific mycolic acids. Although the function of a number of genes involved in fatty acid and mycolic acid biosynthesis is known, information relevant to the initial steps within these biosynthetic pathways is relatively sparse. Interestingly, the genomes of Corynebacterianeae possess a high number of accD genes, whose gene products resemble the beta-subunit of the acetyl-CoA carboxylase of Escherichia coli, providing the activated intermediate for fatty acid synthesis. We present here our studies on four putative accD genes found in C. glutamicum. Although growth of the accD4 mutant remained unchanged, growth of the accD1 mutant was strongly impaired and partially recovered by the addition of exogenous oleic acid. Overexpression of accD1 and accBC, encoding the carboxylase alpha-subunit, resulted in an 8-fold increase in malonyl-CoA formation from acetyl-CoA in cell lysates, providing evidence that accD1 encodes a carboxyltransferase involved in the biosynthesis of malonyl-CoA. Interestingly, fatty acid profiles remained unchanged in both our accD2 and accD3 mutants, but a complete loss of mycolic acids, either as organic extractable trehalose and glucose mycolates or as cell wall-bound mycolates, was observed. These two carboxyltransferases are also retained in all Corynebacterianeae, including Mycobacterium leprae, constituting two distinct groups of orthologs. Furthermore, carboxyl fixation assays, as well as a study of a Cg-pks deletion mutant, led us to conclude that accD2 and accD3 are key to mycolic acid biosynthesis, thus providing a carboxylated intermediate during condensation of the mero-chain and alpha-branch directed by the pks-encoded polyketide synthase. This study illustrates that the high number of accD paralogs have evolved to represent specific variations on the well known basic theme of providing carboxylated intermediates in lipid biosynthesis.  (+info)

Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. (5/403)

In Corynebacterium glutamicum, the activity of aconitase is 2.5-4-fold higher on propionate, citrate, or acetate than on glucose. Here we show that this variation is caused by transcriptional regulation. In search for putative regulators, a gene (acnR) encoding a TetR-type transcriptional regulator was found to be encoded immediately downstream of the aconitase gene (acn) in C. glutamicum. Deletion of the acnR gene led to a 5-fold increased acn-mRNA level and a 5-fold increased aconitase activity, suggesting that AcnR functions as repressor of acn expression. DNA microarray analyses indicated that acn is the primary target gene of AcnR in the C. glutamicum genome. Purified AcnR was shown to be a homodimer, which binds to the acn promoter in the region from -11 to -28 relative to the transcription start. It thus presumably acts by interfering with the binding of RNA polymerase. The acn-acnR organization is conserved in all corynebacteria and mycobacteria with known genome sequence and a putative AcnR consensus binding motif (CAGNACnnncGTACTG) was identified in the corresponding acn upstream regions. Mutations within this motif inhibited AcnR binding. Because the activities of citrate synthase and isocitrate dehydrogenase were previously reported not to be increased during growth on acetate, our data indicate that aconitase is a major control point of tricarboxylic acid cycle activity in C. glutamicum, and they identify AcnR as the first transcriptional regulator of a tricarboxylic acid cycle gene in the Corynebacterianeae.  (+info)

Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease-encoding genes in Corynebacterium glutamicum. (6/403)

The molecular identification of the Corynebacterium glutamicum urea uptake system is described. This ABC-type transporter is encoded by the urtABCDE operon, which is transcribed in response to nitrogen limitation. Expression of the urt genes is regulated by the global nitrogen regulator AmtR, and an amtR deletion strain showed constitutive expression of the urtABCDE genes. The AmtR repressor protein also controls transcription of the urease-encoding ureABCEFGD genes in C. glutamicum. The ure gene cluster forms an operon which is mainly transcribed in response to nitrogen starvation. To confirm the increased synthesis of urease subunits under nitrogen limitation, proteome analyses of cytoplasmic protein extracts from cells grown under nitrogen surplus and nitrogen limitation were carried out, and five of the seven urease subunits were identified.  (+info)

Integration of E. coli aroG-pheA tandem genes into Corynebacterium glutamicum tyrA locus and its effect on L-phenylalanine biosynthesis. (7/403)

AIM: To study the effect of integration of tandem aroG-pheA genes into the tyrA locus of Corynebacterium glutamicum (C. glutamicum) on the production of L-phenylalanine. METHODS: By nitrosoguanidine mutagenesis, five p-fluorophenylalanine (FP)-resistant mutants of C.glutamicum FP were selected. The tyrA gene encoding prephenate dehydrogenase (PDH) of C.glutamicum was amplified by polymerase chain reaction (PCR) and cloned on the plasmid pPR. Kanamycin resistance gene (Km) and the P(BF) -aroG-pheA-T (GA) fragment of pGA were inserted into tyrA gene to form targeting vectors pTK and pTGAK, respectively. Then, they were transformed into C.glutamicum FP respectively by electroporation. Cultures were screened by a medium containing kanamycin and detected by PCR and phenotype analysis. The transformed strains were used for L-phenylalanine fermentation and enzyme assays. RESULTS: Engineering strains of C.glutamicum (Tyr(-)) were obtained. Compared with the original strain, the transformed strain C. glutamicum GAK was observed to have the highest elevation of L-phenylalanine production by a 1.71-fold, and 2.9-, 3.36-, and 3.0-fold in enzyme activities of chorismate mutase, prephenate dehydratase and 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase, respectively. CONCLUSION: Integration of tandem aroG-pheA genes into tyrA locus of C. glutamicum chromosome can disrupt tyrA gene and increase the yield of L-phenylalanine production.  (+info)

Cometabolism of a nongrowth substrate: L-serine utilization by Corynebacterium glutamicum. (8/403)

Despite its key position in central metabolism, L-serine does not support the growth of Corynebacterium glutamicum. Nevertheless, during growth on glucose, L-serine is consumed at rates up to 19.4 +/- 4.0 nmol min(-1) (mg [dry weight])(-1), resulting in the complete consumption of 100 mM L-serine in the presence of 100 mM glucose and an increased growth yield of about 20%. Use of 13C-labeled L-serine and analysis of cellularly derived metabolites by nuclear magnetic resonance spectroscopy revealed that the carbon skeleton of L-serine is mainly converted to pyruvate-derived metabolites such as L-alanine. The sdaA gene was identified in the genome of C. glutamicum, and overexpression of sdaA resulted in (i) functional L-serine dehydratase (L-SerDH) activity, and therefore conversion of L-serine to pyruvate, and (ii) growth of the recombinant strain on L-serine as the single substrate. In contrast, deletion of sdaA decreased the L-serine cometabolism rate with glucose by 47% but still resulted in degradation of L-serine to pyruvate. Cystathionine beta-lyase was additionally found to convert L-serine to pyruvate, and the respective metC gene was induced 2.4-fold under high internal L-serine concentrations. Upon sdaA overexpression, the growth rate on glucose is reduced 36% from that of the wild type, illustrating that even with glucose as a single substrate, intracellular L-serine conversion to pyruvate might occur, although probably the weak affinity of L-SerDH (apparent Km, 11 mM) prevents substantial L-serine degradation.  (+info)

*Corynebacterium glutamicum

Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. Corynebacterium glutamicum ATCC 14067 was ... PMC 3264075 . Genome sequence for C. glutamicum from NCBI. Type strain of Corynebacterium glutamicum at BacDive - the Bacterial ... Corynebacterium glutamicum is a Gram-positive, rod-shaped bacteria which is used industrially for large-scale production of ... 4 September 2003). "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l- ...

*Volutin granules

"Formation of volutin granules in Corynebacterium glutamicum". FEMS Microbiology Letters. Institut fr Biotechnologie (IBT-1), ... the production of which is used as one of the identifying criteria when attempting to isolate Corynebacterium diphtheriae on ...

*Corynebacterial porin B

Porins B and C are cell wall channel-forming proteins from Corynebacterium. Porin B from Corynebacterium glutamicum ( ... Ziegler K, Benz R, Schulz GE (June 2008). "A putative alpha-helical porin from Corynebacterium glutamicum". J. Mol. Biol. 379 ( ...

*Isocitrate dehydrogenase

Chen R, Yang H (November 2000). "A highly specific monomeric isocitrate dehydrogenase from Corynebacterium glutamicum". Arch. ... C. glutamicum favored NADP+ over NAD+. In terms of stability with response to temperature, both enzymes had a similar Tm or ... glutamicum was recorded as having ten times as much activity than E. coli and seven times more affinitive/specific for NADP. ... glutamicum and E. coli, monomer and dimer, respectively, both enzymes were found to "efficiently catalyze identical reactions ...

*Protein production

Brinkrolf, K; Schröder, J; Pühler, A; Tauch, A (2010). "The transcriptional regulatory repertoire of Corynebacterium glutamicum ... "Secretion of human epidermal growth factor by Corynebacterium glutamicum". Lett Appl Microbiol. 42 (1): 66-70. doi:10.1111/j. ... The C. glutamicum species is widely used for producing glutamate and lysine, components of human food, animal feed and ... Non-pathogenic species of the gram-positive Corynebacterium are used for the commercial production of various amino acids. ...

*Corynebacterium efficiens

"Genomic analyses of transporter proteins in Corynebacterium glutamicum and Corynebacterium efficiens". In Eggeling, Lothar; ... "The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium ... Handbook of Corynebacterium glutamicum. pp. 149-86. ISBN 978-1-4200-3969-6. Zhang, R.; Zhang, C.-T. (2005). "Genomic Islands in ... Corynebacterium efficiens at the Encyclopedia of Life LPSN Type strain of Corynebacterium efficiens at BacDive - the Bacterial ...

*Homoserine dehydrogenase

"Characteristics of methionine production by an engineered Corynebacterium glutamicum strain". Metab. Eng. 9 (4): 327-336. doi: ...

*Histidine

"Modification of histidine biosynthesis pathway genes and the impact on production of L-histidine in Corynebacterium glutamicum ... its regulation and biotechnological application in Corynebacterium glutamicum". Microbial Biotechnology. 7 (1): 5-25. doi: ...

*CtRNA

In Corynebacterium glutamicum, it achieves this by antisense pairing with the mRNA of RepB, a replication initiation protein. ... "Control of rep gene expression in plasmid pGA1 from Corynebacterium glutamicum". J Bacteriol. 185 (8): 2402-2409. doi:10.1128/ ...

*D-inositol-3-phosphate glycosyltransferase

"Structural and enzymatic analysis of MshA from Corynebacterium glutamicum: substrate-assisted catalysis". J. Biol. Chem. 283 ( ...

*Transsulfuration pathway

Hwang, B. J.; Yeom, H. J.; Kim, Y.; Lee, H. S. (2002). "Corynebacterium glutamicum utilizes both transsulfuration and direct ...

*ARC3 family

"Efflux permease CgAcr3-1 of Corynebacterium glutamicum is an arsenite-specific antiporter". The Journal of Biological Chemistry ... from Alkaliphilus metalliredigens and Corynebacterium glutamicum". The Journal of Biological Chemistry 284 (30): 19887-19895. ...

*Xerlin

Metabolic Engineering of the Valine Pathway in Corynebacterium Glutamicum - Analysis and Modelling. Germany: Forschungszentrum ...

*Industrial microbiology

The production of these amino acids is due to Corynebacterium glutamicum and fermentation. C.glutamicum was engineered to be ... L-Lysine was originally produced from diaminopimelic acid (DAP) by E.coli, but once the C.glutamicum was discovered for the ... L-Tryptophan is also produced through fermentation and by Corynebacterium and E.coli, though the production is not as large as ... of L-Glutamic acid was 2.2 million tons and is produced using a submerged fermentation technique inoculated with C.glutamicum. ...

*Tryptophan

Becker J, Wittmann C (August 2012). "Bio-based production of chemicals, materials and fuels -Corynebacterium glutamicum as ... glutamicum or E. coli. These strains carry mutations that prevent the reuptake of aromatic amino acids or multiple/ ...

*Porin (protein)

... a channel forming peptide from Corynebacterium glutamicum". FEBS Letters. 587 (22): 3687-3691. doi:10.1016/j.febslet.2013.09. ...

*LysC

It is found in a variety of bacteria, including Bacillus subtilis, Escherichia coli and Corynebacterium glutamicum. It is ...

*Lysine exporter

The superfamily was named based on the early discovery of the LysE carrier protein of Corynebacterium glutamicum. 2.A.75 - The ... LysE of Corynebacterium glutamicum (TC# 2.A.75.1.1) and ArgO of E. coli) have been functionally characterized, but functionally ... topology of the lysine exporter LysE of Corynebacterium glutamicum, a paradyme for a novel superfamily of transmembrane solute ... L-lysine export from Corynebacterium glutamicum". Molecular Microbiology. 22 (5): 815-826. doi:10.1046/j.1365-2958.1996.01527.x ...

*Streptomyces mobaraensis

"High level expression of Streptomyces mobaraensis transglutaminase in Corynebacterium glutamicum using a chimeric pro-region ...

*Fed-batch culture

An example is lysine production with homoserine- or threonine/methionine-requiring mutant of Corynebacterium glutamicum being ...

*Homoserine/threonine resistance transporter

... topology of the lysine exporter LysE of Corynebacterium glutamicum, a paradyme for a novel superfamily of transmembrane solute ...

*Cyanophycin

... of cyanophycin in a number of biotechnologically relevant bacteria such as Escherichia coli and Corynebacterium glutamicum. ...

*Z curve

"A systematic method to identify genomic islands and its applications in analyzing the genomes of Corynebacterium glutamicum and ...

*Microbiology

Corynebacterium glutamicum is one of the most important bacterial species with an annual production of more than two million ...

*Succinic acid fermentation

Corynebacterium glutamicum and Saccharomyces cerevisia. Understanding of the central carbon metabolism of these organisms is ...

*Arnold Demain

... by Corynebacterium glutamicum, since Merck was selling monosodium glutamate (MSG) at the time." In addition, the team managed ...
Corynebacterium glutamicum is a Gram-positive, rod-shaped bacteria which is used industrially for large-scale production of amino acids. While originally identified in a screen for organisms secreting L-glutamate, mutants of C. glutamicum have also been identified which produce various other amino acids. Due to its industrial importance, several clones of C. glutamicum have been sequenced by both industry and academic groups. Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. Corynebacterium glutamicum ATCC 14067 was previously known as Brevibacterium flavum. List of sequenced bacterial genomes Kalinowski, J; Bathe, B; Bartels, D; Bischoff, N; Bott, M; et al. (4 September 2003). "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l-aspartate-derived amino acids and vitamins". Journal of Biotechnology. 104 (1-3): 5-25. doi:10.1016/S0168-1656(03)00154-8. PMID 12948626. Zahoor A; Lindner SN; Wendisch VF (October ...
The surface (S)-layer gene region of the Gram-positive bacterium Corynebacterium glutamicum ATCC 14067 was identified on fosmid clones, sequenced and compared with the genome sequence of C. glutamicum ATCC 13032, whose cell surface is devoid of an ordered S-layer lattice. A 5-97 kb DNA region that is absent from the C. glutamicum ATCC 13032 chromosome was identified. This region includes cspB, the structural gene encoding the S-layer protomer PS2, and six additional coding sequences. PCR experiments demonstrated that the respective DNA region is conserved in different C. glutamicum wild-type strains capable of S-layer formation. The DNA region is flanked by a 7 bp direct repeat, suggesting that illegitimate recombination might be responsible for gene loss in C. glutamicum ATCC 13032. Transfer of the cloned cspB gene restored the PS2(-) phenotype of C. glutamicum ATCC 13032, as confirmed by visualization of the PS2 proteins by SDS-PAGE and imaging of ordered hexagonal S-layer lattices on living ...
The complete genome sequences of greater than 185 microorganisms have been determined, and they are becoming an important resource for the comprehensive understanding of cellular life. Among strains of Corynebacterium glutamicum, bacteria widely used for the industrial production of amino acids, nucleic acids, and organic acids (11, 15), two strains, R (3,314,179 bp) (our unpublished data) and ATCC 13032 (3,309,401 bp [6] or 3,282,708 bp [10]), have been sequenced. Based on whole genome sequences, strain reconstruction studies for improved industrial application have been initiated (21).. By using the genome information of C. glutamicum, we recently found many strain-specific regions existing as "islands" in the common backbone (24). Gene loss and horizontal gene transfer are major genetic processes of genome evolution. These strain-specific islands (SSIs) were possibly shaped on the genome of the ancestral common strain of two C. glutamicum strains by the integration and deletion of many genes. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The secondary glycine-betaine transporter BetP is one of four osmoregulated carriers, which mediate the import of compatible solutes in the Gram-positive soil bacterium Corynebacterium glutamicum under hyperosmotic conditions. BetP serves both as an osmosensor and osmoregulator. Thus the protein has the ability to sense osmotic stress and to regulate its catalytic activity in dependence of the given stress situation. Investigations in proteoliposomes had shown that an elevated internal K+ concentration is the specific stimulus for BetP activation in vitro. In this work a stimulus for an osmosensor identified in vitro could be verified in vivo for the first time, as it was shown that BetP activity depends also in living cell on the presence of potassium. However, the in vivo measurements indicated that beyond K+ a second stimulus is required for osmoresponsive BetP-activation in living cells. It was known that the cytoplasmic C-terminal BetP-domain is essential for potassium sensing. Using ...
Resistance to arsenite (As(III)) by cells is generally accomplished by arsenite efflux permeases from Acr3 or ArsB unrelated families. We analyzed the function of three Acr3 proteins from Corynebacterium glutamicum, CgAcr3-1, CgAcr3-2, and CgAcr3-3. CgAcr3-1 conferred the highest level of As(III) resistance and accumulation in vivo. CgAcr3-1 was also the most active when everted membranes vesicles from Escherichia coli or C. glutamicum mutants were assayed for efflux with different energy sources. As(III) and antimonite (Sb(III)) resistance and accumulation studies using E. coli or C. glutamicum arsenite permease mutants clearly show that CgAcr3-1 is specific for As(III). In everted membrane vesicles expressing CgAcr3-1, dissipation of either the membrane potential or the pH gradient of the proton motive force did not prevent As(III) uptake, whereas dissipation of both components eliminated uptake. Further, a mutagenesis study of CgAcr3-1 suggested that a conserved cysteine and glutamate are ...
The current work deals with the biotechnological synthesis of alpha-ketomethylvalerate (KMV) with Corynebacterium glutamicum. KMV together with the two other branched-chain alpha-keto acids alpha-ketoisovalerate (KIV) and alpha-ketoisocaproate (KIC) is used as a pharmaceutical agent and also as ingredient of functional food. So far KMV has only been produced by chemical synthesis and the aim of this work was to initiate the development of a fermentative KMV production. When C. glutamicum was cultured in the presence of KMV the growth rate and the final cell density were significantly reduced. The experiments also revealed that the branched-chain alpha-keto acids are degraded by C. glutamicum during growth. Specific branched-chain alpha-keto acid converting enzymes could not be identified in C. glutamicum. Analysis of key enzymes of the KMV biosynthesis revealed that the pyruvate condensing reaction of the acetohydroxy acid synthase (AHAS) is competitively inhibited by 100 mM KMV, whereas the ...
Background Methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. Methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes. Results The methanol tolerance of the amino acid producing soil bacterium Corynebacterium glutamicum was improved by experimental evolution in the presence of methanol. The resulting strain Tol1 exhibited significantly increased growth rates in the presence of up to 1 M methanol. However, neither transcriptional changes nor increased enzyme activities of the linear methanol oxidation pathway were observed, which was in accordance with the finding that tolerance to the downstream metabolites formaldehyde and formate was not improved. Genome sequence analysis of strain Tol1 revealed two point mutations potentially relevant to enhanced methanol tolerance: one leading to the amino acid exchange A165T of O-acetylhomoserine ...
The procedure has been used successfully for isolation of different medium-copy-number plasmids carrying pHM1519 or pBL1 origins of replication from Corynebacterium glutamicum ATCC 13032. Yield of plasmid DNA was typically 0.4-1.5 µg per ml LB culture, although yield was dependent on the vector, the insert, and the size of the plasmid ...
l-Ornithine, a non-essential amino acid, has enormous industrial applications in food, pharmaceutical, and chemical industries. Currently, l-ornithine production is focused on microorganism fermentation using Escherichia coli or Corynebacterium glutamicum. In C. glutamicum, development of high l-ornithine producing C. glutamicum was achieved by deletion of argF, but was accompanied by growth deficiency and arginine auxotrophy. l-Arginine has been routinely added to solve this problem; however, this increases production cost and causes feedback inhibition of N-acetyl-l-glutamate kinase activity. To avoid the drawbacks of growth disturbance due to disruption of ArgF, strategies were adopted to attenuate its expression. Firstly, ribosome binding site substitution and start codon replacement were introduced to construct recombinant C. glutamiucm strains, which resulted in an undesirable l-ornithine production titer. Then, we inserted a terminator (rrnB) between argD and argF, which significantly improved l
Previously, we showed that C. glutamicum mycothiol peroxidase MPx, similar to the glutathione peroxidase (Gpx), was resistant to and induced by organic and inorganic peroxides [55]. Moreover, E. faecium gpx is regulated by MarR-type AsrR [44]. Thus, we suggested C. glutamicum MPx was regulated by CosR. The lacZ activity of Pmpx::lacZ chromosomal promoter fusion reporter in relevant C. glutamicum strains and quantitative real-time PCR (qRT-PCR) profiling of mpx expression were quantitatively measured in bacterial cells either untreated or treated with different toxic agents of various concentrations (Figure 5A,B). Concentrations of CHP applied were able to reduce the growth rate but under sub-lethal concentrations (Supplementary Figure S5). As expected, high levels of the promoter lacZ activity of mpx were detected in the ΔcosR strain, regardless of whether or not CHP was present. Under normal conditions (without CHP treatment), the promoter lacZ activity of mpx in ΔcosR strain was 6.5 times ...
Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.
As a gram-positive bacterium with good genomic stability, C. glutamicum is more difficult to engineer than genetically tractable hosts such as E. coli [40, 48]. CRISPR/Cas9-mediated ssDNA recombineering was developed for deleting 400 bp chromosomal fragment in C. glutamicum in the time this manuscript was being prepared [35]. However, gene deletion and insertion with plasmid-borne editing templates that are key enabling techniques for reconstruction and integration of metabolic pathways are still in demand. In this study, a tailor-made CRISPR/Cas9 toolbox was developed for efficient and comprehensive engineering of C. glutamicum. Notably, gene deletion and insertion with plasmid-borne editing templates were efficiently implemented. Moreover, single-nucleotide editing and double-locus editing were achieved at efficiencies of 90.0 and 40.0%, respectively, which will considerably accelerate precise genome editing of C. glutamicum.. S. pyogenes Cas9 is suggested to be toxic to C. glutamicum and ...
2WIT: CRYSTAL STRUCTURE OF THE SODIUM-COUPLED GLYCINE BETAINE SYMPORTER BETP FROM CORYNEBACTERIUM GLUTAMICUM WITH BOUND SUBSTRATE
The construction of microbial cell factories requires cost-effective and rapid strain development through metabolic engineering. Recently, RNA-guided CRISPR technologies have been developed for metabolic engineering of industrially-relevant host. To demonstrate the application of the CRISPR interference (CRISPRi), we developed two-plasmid CRISPRi vectors and applied the CRISPRi in Corynebacterium glutamicum to repress single target genes and double target genes simultaneously. Four-different single genes (the pyc, gltA, idsA, and glgC genes) repressions were successfully performed using the CRISPRi vectors, resulting significant mRNA reductions of the targets compared to a control. Subsequently, the phenotypes for the target gene-repressed strains were analyzed, showing the expected cell growth behaviors with different carbon sources. In addition, double gene repression (the idsA and glgC genes in a different order) by the CRISPRi resulted in an independent gene repression to each target gene
Production of lysine by Corynebacterium glutamicum (PTCC 1532) from different agricultural by-products (molasses and pulpy waste date) was compared to glucose as raw materials. For this purpose, ammonium sulphate was selected as a constant nitrogen source. The effect of different nitrogen sources was also investigated with glucose as a constant carbon source. The production of L-lysine was examined qualitatively and quantitatively using thin layer chromatography (TLC). Results of fermentation experiments showed that the maximum yield corresponded to molasses (48 g L-1) for the fermentation period of 96 hours. For other substrates the yield was lower and the period of fermentation exceeded that for molasses.
Spontaneous prophage induction in a small subpopulation of cells which takes place in the absence of a known stimulus is an often observed, but poorly understood phenomenon. With the proposed project we aim to investigate the impact of stress responses and stochasticity fluctuations of key regulatory proteins on the spontaneous induction of the Corynebacterium glutamicum prophage CGP3 at the single cell level. We aim to combine classical microbiological approaches with stochastic modelling and the design of novel microfluidic devices for single cell studies to contribute to a better understanding of spontaneous prophage induction as a general phenomenon in bacterial populations ...
Page contains details about Corynebacterium glutamicum catalase-gold nanoparticles nanostructured material . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
This chapter describes the technology and provides strategies for molecular strain improvement using current genetic engineering tools, global analysis techniques, and genome-based engineering approaches, with particular emphasis on the industrially important Corynebacterium glutamicum. The transposon (Tn) mutagenesis experiments described in this chapter were performed using special Tn delivery vectors containing insertion sequences (ISs). As a tool for the mutagenesis of C. glutamicum ATCC 13032, Tn vector pAT6100 has been constructed. In C. glutamicum, comparative genomic analysis between two C. glutamicum strains, R and ATCC 13032, revealed that 11 strain-specific islands are scattered in the genomes. Such strain-specific islands are thought to be composed of dispensable genes acquired by horizontal gene transfer. Determining the whole genome sequence of C. glutamicum is aimed at gaining sufficient information to manipulate the metabolism or physiology on a global scale, eventually integrating the
Last September 22, the follow-up meeting of the European project SCILS (http://www.era-ib.net/scils), held in León, brought together European scientists from Germany, UK, Denmark or Norway involved in the research on amino acid production by Corynebacterium glutamicum. The project aims to elucidate the influence of increasing bioreactor inhomogeneities which happen in industrial-scale bioreactors, using this bacterium as workhorse.. ...
A comprehensive overview of C. glutamicum systems biology and biotechnological applications including: proteomics; flux analysis technology; metabolic engineering; manipulation of nitrogen metabolism; transport, degradation and assimilation of aromatic compounds; engineering for production of organic acids and alcohols; production of polyesters; biotechnological applications, biosensors.
The Corynebacterium glutamicum ATCC 13032 prophage CGP3 encodes an actin-like protein, AlpC that was shown to be involved in viral DNA transport and efficient viral DNA replication. AlpC binds to an adapter, AlpA that in turn binds to specific DNA sequences, termed alpS sites. Thus, the AlpAC system is similar to the known plasmid segregation system ParMRS. So far it is unclear how the AlpACS system mediates DNA transport and, whether AlpA and AlpC functionally interact. We show here that AlpA modulates AlpC filamentation dynamics in a dual way. Unbound AlpA stimulates AlpC filament disassembly, while AlpA bound to alpS sites allows for AlpC filament formation. Based on these results we propose a simple search and capture model that explains DNA segregation by viral AlpACS DNA segregation system. ...
The Corynebacterineae comprise a suborder of the Actinomycetales and include most[citation needed] of the acid-fast bacteria. Therewith it is a subgroup of the high GC-content and Gram-positive bacteria. Among its subgroup Mycobacteriaceae are the species which cause tuberculosis and leprosy. The phylogeny is based on 16S rRNA-based LTP release 123 by The All-Species Living Tree Project. The currently accepted taxonomy is based on the List of Prokaryotic Names with Standing in Nomenclature and National Center for Biotechnology Information (NCBI) Order Corynebacteriales Goodfellow & Jones 2015 ["Corynebacteriales" Goodfellow & Jones 2012; Corynebacterineae Stackebrandt et al. 1997 emend. Zhi, Li & Stackebrandt 2009; Mycobacteria] Genus Lawsonella Bell et al. 2016 ["Lawsonella" Nicholson et al. 2015] Genus Tomitella Katayama et al. 2010 Family Corynebacteriaceae Lehmann and Neumann 1907 emend. Zhi, Li & Stackebrandt 2009 (Coryneform bacteria) Genus Corynebacterium Lehmann and Neumann 1896 emend. ...
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Die Universität zu Köln ist eine Exzellenzuniversität mit dem klassischen Fächerspektrum einer Volluniversität. Als eine der größen Hochschulen Europas arbeitet sie in Forschung und Lehre auch international auf höchstem Niveau.
Corynebacterium glutamicum is a nonpathogenic, gram-positive, food-grade microorganism with a long fermentation history, and thus is potentially useful as a host strain for producing a number of recombinant DNA products. We have developed fundamental genetic and genomic tools that enable us to manipulate and redirect pathways involved in central carbon metabolism and amino acid production so that we can understand gene organization, structure and regulation at the molecular level. A better understanding of metabolic signal processes involved in the glucose response could have potential applications to clinical problems such as diabetes.. Biopolymer engineering ...
Corynebacterineae is a suborder of the Actinomycetales, and includes most of the acid-fast bacteria. It is a high G+C gram positive bacteria. It causes Tuberculosis and leprosy. ...
2M47: Solution NMR structure of the Polyketide_cyc-like protein Cgl2372 from Corynebacterium glutamicum, Northeast Structural Genomics Consortium Target CgR160.
The Department congratulates students who received their degrees at the May Commencement ceremony. PhD Hongda Cao, Electron-deficient Conjugated Materials Advisor: Dr. Paul Rupar Wen Chen, Investigation of Protein Dynamics in MshA from Corynebacterium Glutamicum, a Retaining GT-B Glycosyltransferase Advisor: Dr. Patrick Frantom Louis "Chip" Reisman, The Anionic Ring-Opening Polymerization of Cyclic Imines Advisor: Dr. Paul Rupar […]. Read More…. ...
Van Laer, K., A. M. Dziewulska, M. Fislage, K. Wahni, A. Hbeddou, J-F. Collet, W. Versées, L. M. Mateos, V. Tamu Dufe, and J. Messens, NrdH-redoxin of Mycobacterium tuberculosis and Corynebacterium glutamicum dimerizes at high protein concentration and exclusively receives electrons from thioredoxin reductase., J Biol Chem, vol. 288, issue 11, pp. 7942-55, 2013 Mar 15. ...
Authors: Singarapu, Kiran Kumar; sukumaran, Dinesh; Parish, David; Chen, Chen; Kellie, Cunningham; Rong, Xiao; G V T, Swapna; Montelione, Gaetano; Szyperski, Thomas. Citation: Singarapu, Kiran Kumar; Xiao, Rong; Sukumaran, Dinesh; Acton, Thomas; Montelione, Gaetano; Szyperski, Thomas. "NMR structure of protein Cgl2762 from Corynebacterium glutamicum implicated in DNA transposition reveals a helix-turn-helix motif attached to a flexibly disordered leucine zipper." Proteins 70, 1650-1654 (2008).. Assembly members: ...
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Corynebacterium jeikeium, a resident of human skin, is often associated with multidrug resistant nosocomial infections in immunodepressed patients. C. jeikeium K411 belongs to mycolic acid-containing actinomycetes, the mycolata and contains a channel-forming protein as judged from reconstitution experiments with artificial lipid bilayer experiments. The channel-forming protein was present in detergent treated cell walls and in extracts of whole cells using organic solvents. A gene coding for a 40 amino acid long polypeptide possibly responsible for the pore-forming activity was identified in the known genome of C. jeikeium by its similar chromosomal localization to known porH and porA genes of other Corynebacterium strains. The gene jk0268 was expressed in a porin deficient Corynebacterium glutamicum strain. For purification temporarily histidine-tailed or with a GST-tag at the N-terminus, the homogeneous protein caused channel-forming activity with an average conductance of 1.25 nS in 1M KCl ...
Here, C. glutamicum was shown to possess N-acetylglucosaminidase activity that is encoded by cg3158/nagA 2 . Structurally, the NagA2 protein belongs to the family 3 glycoside hydrolases, and among these, the N-acetyl-β-D-glucosaminidases show a selective specificity for GlcNAc as substrate [47] with only few exceptions [48]. N-acetyl-β-D-glucosaminidase activity was assayed with 4-nitrophenyl N,N-diacetyl-β-D-chitobioside as substrate, and about 0.27 mM supported half-maximal activity. In comparison, NagZ from E. coli had a higher Km on the same substrate (0.43 mM) [49], whereas NagZ of B. subtilis showed an about two fold lower Km of 0.11 ± 0.0 mM with 4-methylumbelliferyl-β-GlcNAc as substrate [40].. The role of the NagA2 activity in C. glutamicum is still unclear. Analysis of the C. glutamicum transcriptome revealed that the nagA2 gene is transcribed as leaderless transcript with a relatively low RNA abundance [41]. It is not known whether nagA2 expression is regulated in C. ...
Acetobacter- used in the production of vitamin c. Saccharomyces ceravisae which is a type of yeast - produces the enzyme invertase which converts sucrose into glucose and fructose ( a very sweet sugar found in fruits and honey) to produce the semi liquid centre in some chocolate eggs.. Aspergillus Niger - used to produce citric acid which is used as a flavour enhancer and as a preservative in fizzy drinks. Corynebacterium glutamicum - used to produce glutamicum acid which is turned into msg (a flavour enhancer). Mucor miehei- this fungus produces the enzyme chymosin which is used as an alternative to rennet(from calves stomach) to help milk curdle and office vegetarian cheeses. Bacillus theringiensis- produces a toxin that kills insects , used as a biological control agent, the gene can be put into cotton plants to produce a natural pest resistance.. Plant stanol esters -are chemicals and are used to reduce cholesterol by 10 % produced commercially by using bacteria to convert sterols ( types of ...
casSAR Dugability of B4SMK1 | lldD | L-lactate dehydrogenase - Also known as LLDD_STRM5, lldD. Catalyzes the conversion of L-lactate to pyruvate. Is coupled to the respiratory chain.
The topic „Systemic Microbiology" headed by Prof. Bott performs research in molecular and applied microbiology. The long term aim is a comprehensive understanding of the metabolic and regulatory networks in selected microorganisms such as Corynebacterium glutamicum or Gluconobacter oxydans, which serve as production platforms in White Biotechnology to convert renewable carbon sources into industrially or pharmaceutically used compounds (e.g. amino acids or proteins). Besides microbiological, genetical, and biochemical methods, global tools such genomics, transcriptomics, proteomics and metabolomics are applied. Synthetic biology is used to increase the substrate and product spectrum of the microbial cell factories by adding new metabolic pathways. Novel tools in strain development and single-cell analysis are established based on biosensors and high-throughput methods such as fluorescence-activated cell sorting (FACS). more ...
Research Manager Håvard Sletta - SINTEF Materials and Chemistry, Department of Biotechnology, Trondheim - Norway. The project aims to systematically elucidate the influence of increasing bioreactor inhomogeneity which occurs in industrial-scale bioreactor, with respect to microbial physiology and production performance of Corynebacterium glutamicum, a microorganism with broad industrial applications. Early consideration of inhomogeneity issues during lab scale process development will facilitate the selection of the most potent production strain, accelerate the upscaling process and improve the performance at production scale. Such inhomogeneous conditions can be mimicked at lab scale by a so called scale-down simulator bioreactor, consisting of a well-mixed stirred tank reactor (STR) and a plug-flow reactor (PFR) connected in series to it. During operation the cultivation volume is continuously pumped from the STR through the PFR simulating zones of inhomogeneity known to be present at the ...
Determining the chemical and structural modifications occurring within a protein during fundamental processes such as ligand or substrate binding is essential to building up a complete picture of biological function. Currently, significant unanswered questions relate to the way in which protein structural dynamics fit within the structure-function relationship and to the functional role, if any, of bound water molecules in the active site. Addressing these questions requires a multidisciplinary approach and complementary experimental techniques that, in combination, enhance our understanding of the complexities of protein chemistry. We exemplify this philosophy by applying both physical and biological approaches to investigate the active site chemistry that contributes to the inhibition of the Corynebacterium glutamicum catalase enzyme by nitric oxide. Ultrafast two-dimensional infrared spectroscopy (2D-IR) experiments exploit the NO ligand as a local probe of the active site molecular ...
Search result for Ignat Herrmann: C. Clyde Atkins Et Al., Plaintiffs, V. United States. U.S. Supreme Court Transcript of Record with Supporting Pleadings(9781270660859), Christmas Is Murder(9781503282315), Nutrition : Real People, Real Choices(9780130612243), Menschenrechte für Personen mit Demenz : Soziale und ethische Perspektiven(9783837644951), Clonage Des Genes Soda Et Msra de Corynebacterium Glutamicum(9783838179025), The Mark Stephens Yoga Sequencing Deck(9781623170615), etc... books - Download Ebook Online
By joining ICAAS, you will become a member of the association representing the leading amino acids producers and users. In particular, joining ICAAS provides the opportunities to:. ...
The Raleigh, NC plant of Ajinomoto U.S.A. manufactures amino acids, the integral components of protein. All the plants amino acids are pharmaceutical grade,...
Shop Ketoisovalerate oxidoreductase ELISA Kit, Recombinant Protein and Ketoisovalerate oxidoreductase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
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TY - JOUR. T1 - GntR-Type Transcriptional Regulator pckr Negatively Regulates the Expression of Phosphoenolpyruvate Carboxykinase in Corynebacterium glutamicum. AU - Hyeon, Jeong Eun. AU - Kang, Dae Hee. AU - Kim, Young In. AU - You, Seung Kyou. AU - Han, Sung Ok. PY - 2012/5/1. Y1 - 2012/5/1. N2 - The pck (cg3169) gene of Corynebacterium glutamicum encodes a phosphoenolpyruvate carboxykinase (PEPCK). Here, a candidate transcriptional regulator that binds to the promoter region of pck was detected using a DNA affinity purification approach. An isolated protein was identified to be PckR (Cg0196), a GntR family transcriptional regulator which consists of 253 amino acids with a mass of 27 kDa as measured by peptide mass fingerprinting. The results of electrophoretic mobility shift assays verified that PckR specifically binds to the pck promoter. The putative regulator binding region extended from position-44 to-27 (an 18-bp sequence) relative to the transcriptional start point of the pck gene. We ...
Dear collegues, I´d like to get to know people (scientists, work groups et c.), who work on any topic concerning the genera Corynebacterium or Brevibacterium. As I started my PhD studying Corynebacterium glutamicum (osmotic stress - amino acid production) last year I would be grateful to get in contact with as many people as possible sharing interest in this field of research. However, please don´t hesitate to contact me, if you´re working on the molecular biology level. My aim is to get to know problems you don´t read from in the literature or even share some problem solutions.... Thank you. If reply, please remove the nospam Yours, Hendrik Rönsch University of Cologne, Biochemistry Please visit my homepage: http://come.to/hendrik.roensch ...
Limitation of natural sources, especially of fossil resources, for base material that is currently used to produce polyamides and related composites together with the increasing demand of these products, promotes the search for renewable sources of the base material. Fermentation by genetically engineered bacteria gains increasing interest as one of these possible sources. Cadaverine is a biogenic amine that can be produced by Corynebacterium glutamicum from the amino acid lysine by heterologous expression of a lysine decarboxylase from Escherichia coli. Overexpression of the patA and patD genes from Escherichia coli in Corynebacterium glutamicum enables the latter to further metabolize cadaverine to 5 aminovalerate (5AVA), which is a potential base material for the production of nylon 5 and a C5 platform for the synthesis of base materials for other polyamides. Commercial Opportunities Chemical industry is facing an increasing demand on polyamides and related composites, whereas in contrast the ...
Corynebacteria contain a heme uptake system encoded in hmuTUV genes, in which HmuT protein acts as a heme binding protein to transport heme to the cognate transporter HmuUV. The crystal structure of HmuT from Corynebacterium glutamicum (CgHmuT) reveals that heme is accommodated in the central cleft with His141 and Tyr240 as the axial ligands and that Tyr240 forms a hydrogen bond with Arg242. In this work, the crystal structures of H141A, Y240A, and R242A mutants were determined to understand the role of these residues for the heme binding of CgHmuT. Overall and heme environmental structures of these mutants were similar to those of the wild type, suggesting that there is little conformational change in the heme-binding cleft during heme transport reaction with binding and the dissociation of heme. A loss of one axial ligand or the hydrogen bonding interaction with Tyr240 resulted in an increase in the redox potential of the heme for CgHmuT to be reduced by dithionite, though the wild type was not
(2005) Inui et al. Journal of Molecular Microbiology and Biotechnology. The central metabolic pathway of Corynebacterium glutamicum was engineered to produce ethanol. A recombinant strain which expressed the Zymomonas mobilis genes coding for pyruvate decarboxylase (pd...
This HMM represents a family of proteins, often annotated as a putative IMP dehydrogenase, related to IMP dehydrogenase and GMP reductase and restricted to the high GC Gram-positive bacteria. All species in which a member is found so far (Corynebacterium glutamicum, Mycobacterium tuberculosis, Streptomyces coelicolor, etc.) also have IMP dehydrogenase as described by TIGRFAMs entry TIGR01302 ...
Numerous significant hits in gapped BLAST; e.g. residues 13-367 are 61% similar to 1808696 putative type II 5-cytosoine methyltransferase of Corynebacterium glutamicum, residues 15-338 are 35% similar to 9622224 cytosine-specific methyltransferase of Bacillus sp. LU11, residues 19-338 are 35% similar to emb,CAA59690.1, site-specific DNA-methyltransferase (cytosine-specific) of Haemophilus parahaemolyticus ...
Cog : Sonnen, H., J. Schneider, and H.J. Kutzner. 1990. Corynephage Cog, a virulent bacteriophage of Corynebacterium glutamicum, and its relationship to ΦGA1, an inducible phage particle from Brevibacterium flavum. J. Gen Virol. 71: 1629-1633. ...
«Corynebacterium» Corynebacterium is a genus of Gram-positive, rod-shaped bacteria. They are widely distributed in nature and are mostly innocuous. Some are useful ...
Corynebacterium pseudodiphtheriticum ATCC ® BAA-732™ Designation: Vitek #12653 TypeStrain=False Application: Quality control strain
This post explores the Corynebacterium, a large group of mostly harmless bacteria that holds some surprises. Included is a demonstrated means of removal.
Knowledge of the sequence of a DNA segment has many uses, and some examples follow. First, it can be used to find genes, segments of DNA that code for a specific protein or phenotype. If a region of DNA has been sequenced, it can be screened for characteristic features of genes. For example, open reading frames (ORFs)-long sequences that begin with a start codon (three adjacent nucleotides; the sequence of a codon dictates amino acid production) and are uninterrupted by stop codons (except for one at their termination)-suggest a protein-coding region. Also, human genes are generally adjacent to so-called CpG islands-clusters of cytosine and guanine, two of the nucleotides that make up DNA. If a gene with a known phenotype (such as a disease gene in humans) is known to be in the chromosomal region sequenced, then unassigned genes in the region will become candidates for that function. Second, homologous DNA sequences of different organisms can be compared in order to plot evolutionary ...
INDUSTRIAL PRODUCTION AND CAPACITY UTILIZATION: A REVISION The Federal Reserves index of industrial production (IP) and its related measures of capacity and utilization have been revised principally for the period 1992 to date (chart 1).[1] For the third quarter of 1997, the revision places the production index at 125.2 percent of output in 1992, compared with 121.5 percent reported previously (table 1).[2] The revision places the capacity index at 151.3 percent of output in 1992, compared with 144.6 percent reported previously. The rate of capacity utilization--the ratio of production to capacity--has been revised downward about 1.3 percentage points, to 82.7 percent in the third quarter of 1997. The central aspect of the revision is the updating of the comprehensive annual data and of the revised monthly source data used in the estimation of production, capacity, and utilization. Most important was the 1995 Annual Survey of Manufactures, which provided a stronger picture of the growth in ...
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void:inDataset: http://aims.fao.org/aos/agrovoc/void.ttl#Agrovoc. Created: 2011-11-20T20:02:53Z. Last modified: 2011-11-20T20:02:53Z. skos:notation: 1913 ...
After the first year you can decide to carry on your studies either at NTNU, NMBU or UoI. In the first year your will gain competences in Industrial Production at DTU and in the second year you can decide to carry on your studies either at NTNU, NMBU or HI (UoI). At NTNU you will gain additional competences in Food Biochemistry, at NMBU in Product Development whilst at UoI you will gain additional competences in Supply Chain Management ...
Industrial production to grow by 9% in 2006 . View news feed from Ukrainian Independent Information Agency UNIAN - news about social life for 14 July
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[106 Pages Report] Check for Discount on Global L-Valine Market Professional Survey Report 2016 report by QYResearch Group. This report mainly covers the following Segment regions including (the...
Aerobically cultivated cells of Corynebacterium glutamicum produce mixed organic acids, including succinic acid (SA), from glucose when the cells are transferred to oxygen-deprived conditions. Genetic modification, including inactivation of lactate dehydrogenase and overexpression of pyruvate carboxylase, allows this microbe to be an efficient SA producer under the conditions of oxygen deprivation. High productivity and high titers can be achieved in the production process by using the genetically engineered strain of C. glutamicum under the given conditions. However, glucose consumption for cell preparation decreases process yield (defined as the quantity of SA produced divided by the total quantity of glucose used in cell preparation and SA production). In this study, we investigated cell recycle fed-batch fermentation for SA production to improve the process yield by reducing the effect of glucose consumption for cell preparation on the process yield. A genetically stable and markerless strain,
Corynebacterium minutissimum: …and attributed to the bacterium Corynebacterium minutissimum. The lesions are generally seen on the inner sides of the thighs, in the scrotum, in the toe webs, and in the armpits. Erythrasma is more likely to occur in a warm climate. It is usually effectively treated with broad-spectrum antibiotics, but (on…
134 23. Geier H, et al. (2008) Autoinducer-2 triggers the oxidative stress response in Mycobacterium avium, leading to biofilm formation. Appl. Environ. Microbiol. 74, 1798-1804. 24. Gopalaswamy R, et al. (2008) Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF. FEMS Microbiol. Lett. 278, 121-127. 25. Hanlon WA, et al. (1997) Pkn9, a Ser/Thr protein kinase involved in the development of Myxococcus xanthus. Mol. Microbiol. 23, 459-471. 26. Harper C, et al. (2008) Regulation of nitrogen metabolism in Mycobacterium tuberculosis: a comparison with mechanisms in Corynebacterium glutamicum and Streptomyces coelicolor. IUBMB life 60, 643-650. 27. Houben EN, et al. (2009) Differential expression of a virulence factor in pathogenic and non-pathogenic mycobacteria. Mol. Microbiol. 72, 41-52. 28. Hunter T (1995) Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell 80, 225-236. 29. Jefferson KK ...
Research. I am working as an Assoc. Prof. / senior researcher at the Department of Biotechnology, NTNU. My main research activites are wihtin computational and synthetic biology, and biophysics. In our lab, we employ various methods, such as microfluidics, single-cell arrays, and high-throughput screening. We work with a large sellection of microorganisms including Corynebacterium glutamicum, Escherichia coli, Pseudomonas putida KT2440, psychrophilic Pseudomonas, Psychrobacter spp., Pichia pastoris, Streptomyces albus, S. coelicolor, S. lividans, Synechococcus sp. PCC 7002, Saccharomyces cerevisiae, and Thermus thermophilus.. Projects. EU-H2020 - Advanced toolbox for rapid and cost-effective functional metagenomic screening - microbiology meets microfluidics.. EPSRC-UK - Design the Future 2: Thinking Soils: Engineered bacteria as computational agents in the design and manufacture of new materials and structures. RCN-FORNY2020-NO - Fast-X-Press. NTNU-Discovery - SUPERAP - fast track for efficient ...
Discrimination of Corynebacterium glutamicum, Brevibacterium flavum and Brevibacterium lactofermentum by restriction pattern analysis of DNA adjacent to the hom gene ...
1. The major free lipids of Corynebacterium aquaticum were characterized as dimannosyl diglyceride, monomannophosphoinositide and phosphatidylethanolamine. Bisphosphatidylglycerol and phosphatidylglycerol were also tentatively identified. 2. We regard this as the only well-documented case of an organism containing monomannophosphoinositide to the exclusion of dimannophosphoinositides and the higher homologues. 3. The co-existence of the two mannolipids in one organism is a distinctive feature. So also is the presence of phosphatidylethanolamine in a corynebacterium. 4. The monomannophosphoinositide apparently does not utilize phosphatidylinositol as a precursor, unlike the monomannophosphoinositide of Propionibacterium shermanii. CDP-diglyceride may be necessary for its synthesis.. ...
Sepsis with a previously undescribed species of Corynebacterium was documented in four patients. All patients had predisposing illness at the time of infection, three patients having leukemia in relapse and one having a porencephalic cyst and a ventriculoatrial shunt. The isolates from blood cultures had a characteristic metallic sheen when grown on blood agar. They were resistant to most antibiotics tested, including the penicillins, but were uniformly sensitive to vancomycin. Common biochemical characteristics, the metallic sheen, and the unusual antibiotic sensitivity pattern suggest that these isolates comprise a new species or group of closely related species of Corynebacterium that is capable of infection in man. ...
Cytokinin application to dormant buds will cause them to develop. A witches broom is caused by a pathogen such as the bacterium Corynebacterium fascians (or A. tumefaciens) that produces cytokinin which, in turn, causes stimulates lateral bud development (branching). These results suggest that apical dominance may be related to cytokinin, too.. For example, when tobacco cells are infected with the Ti-plasmid that has been modified to possess the heat shock promoter, a heat treatment stimulates the cells to produce increased amounts of cytokinin. These plants exhibit less apical dominance and remain green longer than non-heat treated controls. Thus, these results support the conclusion that senescence and apical dominance are related to cytokinin levels.. G. Promote cell ...
Genomics: Corynebacterium diphtheriae: chromosome 2,488,635 bp; 2320 predicted ORFs (Cerdeno-Tarraga et al. 2003) Cell morphology: Rod-shaped cells; irregular, club-shaped ( Coryne), or V-shaped...
Corynebacterium renale ATCC ® 19412™ Designation: NCTC 7448 TypeStrain=True Application: Quality control strain Quality control for API Coryne
Get an answer for What is the morphology of the bacterial cells Corynebacterium diptheriae and Staphylococcus aureus? What is the relevance of the Gram staining procedure as a diagnostic tool? and find homework help for other Science questions at eNotes
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Kotte, Oliver; Volkmer, Benjamin; Radzikowski, Jakub L; Heinemann, Matthias (2014). Phenotypic bistability in Escherichia colis central carbon metabolism. Molecular Systems Biology, 10:736.. Zampar, Guillermo G; Kümmel, Anne; Ewald, Jennifer; Jol, Stefan; Niebel, Bastian; Picotti, Paola; Aebersold, Ruedi; Sauer, Uwe; Zamboni, Nicola; Heinemann, Matthias (2013). Temporal system-level organization of the switch from glycolytic to gluconeogenic operation in yeast. Molecular Systems Biology, 9:online.. Jol, Stefan J; Kümmel, Anne; Terzer, Marco; Stelling, Jörg; Heinemann, Matthias (2012). System-Level Insights into Yeast Metabolism by Thermodynamic Analysis of Elementary Flux Modes. PLoS Computational Biology, 8(3):e1002415.. Huberts, Daphne H E W; Niebel, Bastian; Heinemann, Matthias (2011). A flux-sensing mechanism could regulate the switch between respiration and fermentation. FEMS Yeast Research, 12(2):118-128.. Costenoble, Roeland; Picotti, Paola; Reiter, Lukas; Stallmach, Robert; ...
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BioEnergy International, LLC ("BioEnergy"), a developer of biorefineries and proprietary technologies to produce specialty chemicals and fuels from renewable feedstocks, grains and cellulosic wastes, announced today that it has signed an agreement to license one of its proprietary biocatalysts to Purac Biochem N.V., the largest lactic acid producer in the world. BioEnergy has an exclusive research agreement with the University of Florida and Dr. Lonnie Ingram to develop technologies to produce certain biorefined specialty chemicals from sugars and cellulose ...
... historical data, charts, stats and more. Finland Change in Industrial Production is at 0.10%, compared to 0.70% last month and -0.60% last year. This is lower than the long term average of 1.27%..
Thermo Scientific™ Culti-Loops™ are ready-to-use QC organisms recommended for use in performance testing of media, stains, reagents and identification kits, and for the evaluation of bacteriological procedures.
Lineage: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium ...
Lineage: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae; Corynebacterium; Corynebacterium ...
The Federal Reserve has revised the index of industrial production (IP) and the related measures of capacity and capacity utilization. Although rates of change from January 1972 through May 2010 are affected, the revision had its largest effect on data from 2006 through 2009. The annual data from the 2007 Census of Manufactures and 2008 Annual Survey of Manufactures (ASM) were the largest sources of revision and implied noticeably stronger output in 2007 (mostly in durable goods industries) and a larger drop in output in 2008 (mostly in nondurables). Nevertheless, the overall contour of total IP in recent years was little changed by the revision: The index increased at a moderate rate in 2006 and 2007; it fell sharply in 2008 and declined further in the first half of 2009. Relative to earlier estimates, measured from fourth quarter to fourth quarter, total IP is now reported to have increased 0.7 percentage point and 0.5 percentage point more rapidly in 2006 and 2007, respectively. The decrease ...
Thermaltake is thrilled to announce a new model to the classic Level 20 collection- the Level 20 GT ARGB Black Edition Full Tower Chassis. Like the Level 20 GT ARGB, the chassis comes with two 200 mm ...
Japans industrial output declined in September compared to that in the previous month, indicating that the weak global demand is continuing to have its impact on the countrys economy.
LDHA - LDHA (untagged)-Human lactate dehydrogenase A (LDHA), transcript variant 1 available for purchase from OriGene - Your Gene Company.
1 The dispositive issue on appeal is whether the thrice incarnated affidavit of merit requirement found in Okla. Stat. tit. 12, 19.1 (Supp. 2013), is unconstitutional. In the wake of Zeier v. Zimmer, 2006 OK 98, 152 P.3d 861, its sequel Wall v. Marouk, 2013 OK 36, 302 P.3d 775, and upon reexamination of the Oklahoma Constitution, the inevitable conclusion is that section 19.1 is an impermissibl... More... $0 (10-25-2017 - OK) ...
To book your IPSA Omnilux light-therapy consultation, simply call the IPSA skin clinic or book online for a same-day appointment.
Argentinas industrial production rose a nonseasonally-adjusted 15% in March from February and rose 3.8% compared to the year-earlier month.
Sed ut perspiciatis, unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam eaque ipsa, quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt, explicabo. Nemo enim ipsam voluptatem.... Read more ...
Sed ut perspiciatis, unde omnis iste natus error sit voluptatem accusantium doloremque laudantium, totam rem aperiam eaque ipsa, quae ab illo inventore veritatis et quasi architecto beatae vitae dicta sunt, explicabo. Nemo enim ipsam voluptatem.... Read more ...
Looking for Corynebacterium parvum? Find out information about Corynebacterium parvum. A genus of gram-positive, straight or slightly curved rods in the coryneform group of bacteria; club-shaped swellings are common; includes human and animal... Explanation of Corynebacterium parvum
Corynebacterium argentoratense is part of the human skin microbiota and is occasionally detected in the upper respiratory tract of patients suffering from tonsillitis. The complete DNA sequence of the type strain DSM 44202 comprises 2,031,902 bp, yielding the smallest genome sequenced thus far for a corynebacterium associated with humans. ...
Corynebacterium minutissimum symptoms, causes, diagnosis, and treatment information for Corynebacterium minutissimum (Erythrasma) with alternative diagnoses, full-text book chapters, misdiagnosis, research treatments, prevention, and prognosis.
Industrial Production in Nicaragua is expected to be 4.29 percent by the end of this quarter, according to Trading Economics global macro models and analysts expectations. Looking forward, we estimate Industrial Production in Nicaragua to stand at 4.29 in 12 months time. In the long-term, the Nicaragua Industrial Production is projected to trend around 4.29 percent in 2020, according to our econometric models. In Nicaragua, industrial production measures the output of businesses integrated in industrial sector of the economy such as manufacturing, mining, and utilities. This page provides - Nicaragua Industrial Production - actual values, historical data, forecast, chart, statistics, economic calendar and news.
Diphtheria toxin, molecular model. Diphtheria is caused by the bacterium Corynebacterium diphtheriae. Symptoms include sore throat, fever and breathing difficulties. - Stock Image F009/6155
Diphtheria is an infectious disease caused by the bacterium Corynebacterium diphtheria. The bacteria primarily infects the mucosal membranes....
Diphtheria toxin (DT) is produced by toxigenic strains of the human pathogen Corynebacterium diphtheriae as well as zoonotic C. ulcerans and C. pseudotuberculosis. Toxigenic strains may cause severe respiratory diphtheria, myocarditis, neurological damage or cutaneous diphtheria. The DT encoding tox gene is located in a mobile genomic region and tox variability between C. diphtheriae and C. ulcerans has been postulated based on sequences of a few isolates. In contrast, species-specific sequence analysis of the diphtheria toxin repressor gene (dtxR), occurring both in toxigenic and non-toxigenic Corynebacterium species, has not been done yet. We used whole genome sequencing data from 91 toxigenic and 46 non-toxigenic isolates of different pathogenic Corynebacterium species of animal or human origin to elucidate differences in extracted DT, DtxR and tox-surrounding genetic elements by a phylogenetic analysis in a large sample set. Sequences of both DT and DtxR, extracted from whole genome sequencing data,
More specific detection methods in recent years have allowed further investigation of the coryneform bacterias.C macginleyi was first identified in 1995 by Riegel et al 5-7 during investigations on lipophilic corynebacteria. It has been uniquely isolated from ocular surfaces. The first 18 cases ofC macginleyi conjunctivitis have been detected in Switzerland.8 Within the recent past we found in 10 patients 13 cases of C macginleyiconjunctivitis in Germany, indicating that the presence of this micro-organism is not geographically limited.. Thiel et al report on increasing percentage of patients positive for corynebacteria.10 11 We found in our patients 18.7% Staphylococcus aureus, 12.1% Corynebacterium macginleyi, and 8.4%Haemophilus influenzae. Fahmiet al, however, found coagulase negative staphylococci in 82% and corynebacteria in 58% of their mainly elderly patients.12 In our study group we foundC macginleyi predominantly in middle aged patients without any preference regarding sex. This is in ...
Specific bacterial commensals demonstrating multidrug resistance (MDR) are opportunistic pathogens for immunocompromised patients, including Corynebacterium species (spp.). Severe infections due to MDR corynebacteria are being increasingly reported where several MDR phenotypes have been described. One such phenotype, the macrolide-lincosamide-streptogramin B phenotype (MLSB), is characterized by high-level resistance to macrolides, lincosamides, and streptogramin B. Resistance is thought to be attributable to acquisition of the ermX gene, a methyltransferase that alters the ribosomal macrolide binding site. Until recently, ermX had been reported in only six Corynebacterium spp. We have observed other corynebacteria can also display high-level resistance to MLSB antimicrobials and are ermX positive. Hypotheses being tested include: 1) high-level macrolide and lincosamide resistance in Corynebacterium spp. is caused by acquiring ermX; 2) distribution of ermX is more widespread than previously ...
Diphtheria is a dangerous respiratory disease is caused by a potent toxin produced by certain strains of the bacterium Corynebacterium diphtheriae. Diphtheria is extremely contagious through coughing or sneezing. Risk factors include crowding, poor hygiene, and lack of immunization.. Symptoms usually appear within a week of infection. This infection is characterized by a sore throat, coughing and fever very similar to many common diseases like strep throat. Additional symptoms may be bloody, watery discharge from the nose and rapid breathing. However, a presumptive diagnosis can be made by observing a characteristic thick grayish patch (membrane) found in the throat. In more severe cases, neck swelling and airway obstruction may be observed. In the tropics, cutaneous and wound diphtheria is much more common and can be a source of transmission.. Vacation Home Rentals in beautiful St. Pete - Clearwater, Florida starting from $90 at TurnKey Vacation Rentals.. The real serious danger is when the ...
BrunchClust produces 7 clusters: two complete for ATP-A and ATP-B and one incomplete for ATP-F. ATP-A and ATP-B clusters contain paralogs that are also reported as a result of clustering. There are two paralogs on the ATP-A branch one is of Rhodopirellula baltica and the second is of Methanosarcina acetivorans, and there are three paralogs on the ATP-B branch: two are from the same species as those on the ATP-A branch, i.e. Rhodopirellula baltica and Methanosarcina acetivoran, and the third is from Chlorobium tepidum. List of 30 taxa: 16 Bacteria: Aquifex aeolicus, Bacillus subtilis, Chlorobium tepidum, Corynebacterium glutamicum, Deinococcus radiodurans, Geobacillus kaustophilus, Geobacter sulfurreducens, Gloeobacter violaceus, Nostoc sp., Pseudomonas aeruginosa, Rhodopirellula baltica, Rhodopseudomonas palustris, Streptococcus thermophilus, Streptomyces coelicolor, Thermotoga maritime, Thermus thermophilus, and 14 Archaea: Aeropyrum pernix,Archaeoglobus fulgidus,Haloarcula marismortui, ...

Corynebacterium glutamicum - WikipediaCorynebacterium glutamicum - Wikipedia

Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. Corynebacterium glutamicum ATCC 14067 was ... PMC 3264075 . Genome sequence for C. glutamicum from NCBI. Type strain of Corynebacterium glutamicum at BacDive - the Bacterial ... Corynebacterium glutamicum is a Gram-positive, rod-shaped bacteria which is used industrially for large-scale production of ... 4 September 2003). "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l- ...
more infohttps://en.wikipedia.org/wiki/Corynebacterium_glutamicum

ureB - Urease subunit beta - Corynebacterium glutamicum (strain R) - ureB gene & proteinureB - Urease subunit beta - Corynebacterium glutamicum (strain R) - ureB gene & protein

sp,A4QA22,URE2_CORGB Urease subunit beta OS=Corynebacterium glutamicum (strain R) OX=340322 GN=ureB PE=3 SV=1 ... Corynebacterium glutamicum (strain R). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_ ...
more infohttps://www.uniprot.org/uniprot/A4QA22

Large-Scale Engineering of the Corynebacterium glutamicum Genome | Applied and Environmental MicrobiologyLarge-Scale Engineering of the Corynebacterium glutamicum Genome | Applied and Environmental Microbiology

Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... Large-Scale Engineering of the Corynebacterium glutamicum Genome. Nobuaki Suzuki, Satoshi Okayama, Hiroshi Nonaka, Yota Tsuge, ... The Corynebacterium glutamicum genome: features and impacts on biotechnological processes. Appl. Microbiol. Biotechnol. 62:99- ...
more infohttps://aem.asm.org/content/71/6/3369?ijkey=b3f272b52f13b7c69728837f62a2157e6969b290&keytype2=tf_ipsecsha

Der Regulationsmechanismus des Osmosensors BetP aus Corynebacterium glutamicum - Kölner UniversitätsPublikationsServerDer Regulationsmechanismus des Osmosensors BetP aus Corynebacterium glutamicum - Kölner UniversitätsPublikationsServer

Ott, Vera Magdalena (2008) Der Regulationsmechanismus des Osmosensors BetP aus Corynebacterium glutamicum. PhD thesis, ... which mediate the import of compatible solutes in the Gram-positive soil bacterium Corynebacterium glutamicum under ... die in dem Gram-positiven Bodenbakterium Corynebacterium glutamicum den Import von kompatiblen Soluten unter hyperosmotischen ...
more infohttp://kups.ub.uni-koeln.de/2502/

Corynebacterium glutamicum - microbewikiCorynebacterium glutamicum - microbewiki

Corynebacterium glutamicum Description and significance. C. glutamicum is a small, non-moving Gram-positive soil bacterium. ... "Corynebacterium glutamicum as a model bacterium for the bioremediation of arsenic". International Microbiology. 2006. p. 207- ... "Reduced Folate Supply as a Key to Enhanced L-Serine Production by Corynebacterium glutamicum." Applied and Environmental ... Another possible use for C. glutamicum is in bioremediation, such as for arsenic. C. glutamicum contains two operons in its ...
more infohttps://microbewiki.kenyon.edu/index.php/Corynebacterium_glutamicum

Metabolic engineering of Corynebacterium glutamicum for anthocyanin production | SpringerLinkMetabolic engineering of Corynebacterium glutamicum for anthocyanin production | SpringerLink

Engineering Corynebacterium glutamicum for isobutanol production. Appl Microbiol Biotechnol. 2010;87:1045-55.CrossRefPubMed ... Metabolic engineering of Corynebacterium glutamicum for l-arginine production. Nat Commun. 2014;5:4618.CrossRefPubMedGoogle ... Corynebacterium glutamicum, having been widely used in industrial production of amino acids such as L-glutamate and l-lysine [ ... Bio-based production of organic acids with Corynebacterium glutamicum. Microb Biotechnol. 2013;6:87-102.CrossRefPubMedGoogle ...
more infohttps://link.springer.com/article/10.1186%2Fs12934-018-0990-z

Phenol Biodegradation by Corynebacterium glutamicum Encapsulated in Electrospun FibersPhenol Biodegradation by Corynebacterium glutamicum Encapsulated in Electrospun Fibers

... developed a model system for the biodegradation of phenol-contaminated wastewater by the bacterium Corynebacterium glutamicum. ... A. Nardi, R. Avrahami, E. Zussman, J. Rokem and C. Greenblatt, "Phenol Biodegradation by Corynebacterium glutamicum ... Is Involved in Intercellular Communication of Corynebacterium glutamicum," Archives of Microbiology, Vol. 182, No. 4, 2004, pp ... developed a model system for the biodegradation of phenol-contaminated wastewater by the bacterium Corynebacterium glutamicum. ...
more infohttps://www.scirp.org/journal/PaperInformation.aspx?PaperID=17442

lexA - LexA repressor - Corynebacterium glutamicum ATCC 14067 - lexA gene & proteinlexA - LexA repressor - Corynebacterium glutamicum ATCC 14067 - lexA gene & protein

Corynebacterium glutamicum (strain R). Corynebacterium glutamicum (Brevibacterium saccharolyticum). Corynebacterium glutamicum ... Corynebacterium glutamicum ATCC 14067. Corynebacterium crenatum MT. [Brevibacterium] flavum. Corynebacterium glutamicum (strain ... Corynebacterium glutamicum (Brevibacterium saccharolyticum). Corynebacterium glutamicum ATCC 14067. Corynebacterium crenatum MT ... Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025). Corynebacterium glutamicum ( ...
more infohttp://www.uniprot.org/uniprot/A0A072Z841

Synthetic biology approaches to access renewable carbon source utilization in Corynebacterium glutamicum | SpringerLinkSynthetic biology approaches to access renewable carbon source utilization in Corynebacterium glutamicum | SpringerLink

... glutamicum), an important industrial workhorse, is capable of efficiently producing a variety of value-added chemicals and ... Corynebacterium glutamicum (C. glutamicum), an important industrial workhorse, is capable of efficiently producing a variety of ... Corynebacterium glutamicum Renewable sources Synthetic biology Metabolic engineering This is a preview of subscription content ... Yang J, Zhu Y, Men Y, Sun S, Zeng Y, Zhang Y, Sun Y, Ma Y (2016) Pathway construction in Corynebacterium glutamicum and strain ...
more infohttps://link.springer.com/article/10.1007%2Fs00253-018-9358-x

New Trends in Corynebacterium glutamicum: Beyond the Amino Acids - Nova Science PublishersNew Trends in Corynebacterium glutamicum: Beyond the Amino Acids - Nova Science Publishers

New Trends in Corynebacterium glutamicum: Beyond the Amino Acids. Carlos Barreiro (Editor). Instituto de Biotecnología de León ... Use of Corynebacterium glutamicum for Heavy Metals Bioremediation: Arsenic as Prototype. (Almudena F. Villadangos, José A. Gil ... Home / Shop / Books / Science and Technology / Life Sciences / New Trends in Corynebacterium glutamicum: Beyond the Amino Acids ... L-Valine production in Corynebacterium glutamicum. (Kazumi Hiraga, Crispinus A. Omumasaba, Masayuki Inui, Research Institute of ...
more infohttps://novapublishers.com/shop/new-trends-in-corynebacterium-glutamicum-beyond-the-amino-acids/

Towards methionine overproduction in Corynebacterium glutamicum--methanethiol and dimethyldisulfide as reduced sulfur sources. ...Towards methionine overproduction in Corynebacterium glutamicum--methanethiol and dimethyldisulfide as reduced sulfur sources. ...

... methanethiol and dimethyldisulfide were investigated as sulfur source for methionine synthesis in Corynebacterium glutamicum. ... In vitro studies with the C. glutamicum wild type revealed a relatively low activity of MetY for methanethiol (63 mU/mg) and ... Corynebacterium glutamicum/genetics. *Corynebacterium glutamicum/growth & development. *Corynebacterium glutamicum/metabolism* ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/20798582?dopt=Abstract

Methioninaufnahme und -export in Corynebacterium glutamicum  - Kölner UniversitätsPublikationsServerMethioninaufnahme und -export in Corynebacterium glutamicum - Kölner UniversitätsPublikationsServer

Methionine uptake and excretion in Corynebacterium glutamicum. The characterization of methionine uptake in C. glutamicum ... Methioninaufnahme und -export in Corynebacterium glutamicum. Die Untersuchung der Methioninaufnahme in C. glutamicum führte zur ... Um den Methioninexport in C. glutamicum zu charakterisieren, wurde ein Beladungssystem mit L-Methionin-haltigen Dipeptiden ... glutamicum. The expression of this gene cluster encoding an ABC transporter is regulated by the repressor McbR. The second ...
more infohttps://kups.ub.uni-koeln.de/1529/

Isolation of plasmid DNA from Corynebacterium glutamicum using the QIAGEN Plasmid Mini KitIsolation of plasmid DNA from Corynebacterium glutamicum using the QIAGEN Plasmid Mini Kit

Isolation of plasmid DNA from Corynebacterium glutamicum using the QIAGEN® Plasmid Mini Kit - (EN). ... of different medium-copy-number plasmids carrying pHM1519 or pBL1 origins of replication from Corynebacterium glutamicum ATCC ...
more infohttps://www.qiagen.com/uz/resources/resourcedetail?id=1ad9b140-880d-44c3-ac9f-110cbaae599e&lang=en

The DeoR-Type Regulator SugR Represses Expression of ptsG in Corynebacterium glutamicum | Journal of BacteriologyThe DeoR-Type Regulator SugR Represses Expression of ptsG in Corynebacterium glutamicum | Journal of Bacteriology

The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium ... Corynebacterium glutamicum is a high-G+C content gram-positive soil bacterium which was isolated in 1957 as an l-glutamate- ... The DeoR-Type Regulator SugR Represses Expression of ptsG in Corynebacterium glutamicum Verena Engels, Volker F. Wendisch ... Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. J. Biol. Chem. 280:585- ...
more infohttps://jb.asm.org/content/189/8/2955?ijkey=15624fb43155158011fe764c3516ac5c8f1fac18&keytype2=tf_ipsecsha

Účast alternativních sigma faktorů RNA polymerasy při regulaci exprese genů Corynebacterium glutamicumÚčast alternativních sigma faktorů RNA polymerasy při regulaci exprese genů Corynebacterium glutamicum

Gram-pozitivní, aerobní bakterie Corynebacterium glutamicum je významným producentem aminokyselin. V genomu C. glutamicum se ... Účast alternativních sigma faktorů RNA polymerasy při regulaci exprese genů Corynebacterium glutamicum. *Detail práce ... Corynebacterium glutamicum, sigma faktor, anti-sigma faktor, promotor, tepelný šok, in-vitro ... The role of alternative sigma factors of RNA polymerase in regulation of gene expression in Corynebacterium glutamicum ...
more infohttps://is.cuni.cz/webapps/zzp/detail/200768/?lang=cs

African Journal of Biotechnology  - metabolic engineering of corynebacterium glutamicum to enhance l-leucine production:...African Journal of Biotechnology - metabolic engineering of corynebacterium glutamicum to enhance l-leucine production:...

Key words: Corynebacterium glutamicum, L-leucine, metabolic engineering, fermentation, industrial production. ... A recombinant C. glutamicum strain was constructed by expressing a feedback-resistant leuA-encoded 2-isopropylmalate synthase ( ... IPMS) that carries three amino acid exchanges (R529H, G532D and L535V) from the mutant strain C. glutamicum ML1-9 which was ... This work aimed to develop an efficient L-leucine industrial production strain of Corynebacterium glutamicum by using metabolic ...
more infohttps://academicjournals.org/journal/AJB/article-references/12183F164057

l-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum | Applied and Environmental...l-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum | Applied and Environmental...

l-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum. Bastian Blombach, Mark E. ... l-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum. Bastian Blombach, Mark E. ... l-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum. Bastian Blombach, Mark E. ... l-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Message Subject (Your Name) has ...
more infohttps://aem.asm.org/content/73/7/2079/article-info

Fermentation | Free Full-Text | Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant...Fermentation | Free Full-Text | Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant...

... glutamicum under the given conditions. However, glucose consumption for cell preparation decreases process yield (defined as ... Aerobically cultivated cells of Corynebacterium glutamicum produce mixed organic acids, including succinic acid (SA), from ... Keywords: Corynebacterium glutamicum; oxygen deprivation; succinic acid; cell recycle reaction Corynebacterium glutamicum; ... Improving Process Yield in Succinic Acid Production by Cell Recycling of Recombinant Corynebacterium glutamicum. Toru Jojima 1 ...
more infohttp://www.mdpi.com/2311-5637/2/1/5

RCSB PDB - 2JN6: Solution NMR structure of Protein Cgl2762 from Corynebacterium Glutamicum: Northeast Structural Genomics...RCSB PDB - 2JN6: Solution NMR structure of Protein Cgl2762 from Corynebacterium Glutamicum: Northeast Structural Genomics...

Solution NMR structure of Protein Cgl2762 from Corynebacterium Glutamicum: Northeast Structural Genomics Consortium Target CgR3 ... Find proteins for Q8NM20 (Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)) ... Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025). Mutation(s): 0 ... Organism(s): Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) ...
more infohttps://www.rcsb.org/structure/2jn6

Corynebacterium glutamicum: From Systems Biology to Biotechnological ApplicationsCorynebacterium glutamicum: From Systems Biology to Biotechnological Applications

... glutamicum systems biology and biotechnological applications including: proteomics; flux analysis technology; metabolic ... Corynebacterium glutamicum is most widely known for its role in the industrial production of L-glutamate and L-lysine and as a ... Corynebacterium glutamicum is often used as a model organism because of its ability to produce various useful substances. For ... Corynebacterium glutamicum is well known for the multi-million-ton-scale production of L-glutamate and L-lysine in ...
more infohttps://www.caister.com/cory2

The Activity Study of Aminodeoxychorismate Synthase of Differernt Corynebacterium glutamicum--《China Biotechnology》2009年08期The Activity Study of Aminodeoxychorismate Synthase of Differernt Corynebacterium glutamicum--《China Biotechnology》2009年08期

... from a L-serine producing strain Corynebacterium glutamicum SYPS-062 and model strain Corynebacterium glutamicum ATCC 13032 ... from a L-serine producing strain Corynebacterium glutamicum SYPS-062 and model strain Corynebacterium glutamicum ATCC 13032 ... The Activity Study of Aminodeoxychorismate Synthase of Differernt Corynebacterium glutamicum. REN Jian-hong1,2 ZHANG Xiao-mei1 ... 1 Factors on the Biosynthesis of Valine in a Valine Producer Corynebacterium glutamicum AATV341[J];Journal of Wuxi University ...
more infohttp://en.cnki.com.cn/Article_en/CJFDTotal-SWGJ200908012.htm

RCSB PDB - 2WIT: CRYSTAL STRUCTURE OF THE SODIUM-COUPLED GLYCINE BETAINE SYMPORTER BETP FROM CORYNEBACTERIUM GLUTAMICUM WITH...RCSB PDB - 2WIT: CRYSTAL STRUCTURE OF THE SODIUM-COUPLED GLYCINE BETAINE SYMPORTER BETP FROM CORYNEBACTERIUM GLUTAMICUM WITH...

CRYSTAL STRUCTURE OF THE SODIUM-COUPLED GLYCINE BETAINE SYMPORTER BETP FROM CORYNEBACTERIUM GLUTAMICUM WITH BOUND SUBSTRATE ... Find proteins for P54582 (Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)) ... Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025). Mutation(s): 3 Gene Names: betP ... Organism(s): Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) ...
more infohttps://www.rcsb.org/structure/2WIT

The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived...The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived...

The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived ... The complete genomic sequence of Corynebacterium glutamicum ATCC 13032, well-known in industry for the production of amino ... The C. glutamicum genome was found to consist of a single circular chromosome comprising 3282708 base pairs. Several DNA ... These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/12948626?dopt=Abstract

CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum | Biochemical Journal |...CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum | Biochemical Journal |...

The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium ... Corynebacterium diphtheriae, Corynebacterium jeikeium, and mycobacterium [1-3]. C. glutamicum cells are frequently confronted ... CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum Meiru Si Meiru Si ... Corynebacterium glutamicum is a high G+C-content aerobic Gram-positive bacterium. It is not only a workhorse in biotechnology ...
more infohttps://portlandpress.com/biochemj/article/475/24/3979/49840/CosR-is-an-oxidative-stress-sensing-a-MarR-type

Pyruvate:Quinone Oxidoreductase in Corynebacterium glutamicum: Molecular Analysis of the pqo Gene, Significance of the Enzyme,...Pyruvate:Quinone Oxidoreductase in Corynebacterium glutamicum: Molecular Analysis of the pqo Gene, Significance of the Enzyme,...

Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif. Microbiology 142:1297- ... The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived ... 3). Whereas C. glutamicum WT ΔaceEΔpqo carrying pEKEx2 showed maximal final OD600s of 0.8 to 1.0, C. glutamicum WT ΔaceEΔpqo ... Control of rep gene expression in plasmid pGA1 from Corynebacterium glutamicum. J. Bacteriol. 185:2402-2409. ...
more infohttps://jb.asm.org/content/188/4/1341?ijkey=24e1707032fa49ea8e19ff575eaea8b2d27213f6&keytype2=tf_ipsecsha
  • We chose to delete each of 11 SSIs that are larger than 10 kb among thousands of C. glutamicum R strain-specific islands. (asm.org)
  • Corynebacterium glutamicum is a Gram-positive, rod-shaped bacteria which is used industrially for large-scale production of amino acids. (wikipedia.org)
  • While originally identified in a screen for organisms secreting L-glutamate, mutants of C. glutamicum have also been identified which produce various other amino acids. (wikipedia.org)
  • The engineering of Corynebacterium glutamicum is important for enhanced production of biochemicals. (asm.org)
  • Due to its industrial importance, several clones of C. glutamicum have been sequenced by both industry and academic groups. (wikipedia.org)
  • The Glycosylated Cell Surface Protein Rpf2, Containing a Resuscitation-Promoting Fac- tor Motif, Is Involved in Intercellular Communication of Corynebacterium glutamicum," Archives of Microbiology, Vol. 182, No. 4, 2004, pp. 299-312. (scirp.org)
  • Packed with practical information and state-of-the-art science this concise volume is an essential handbook for everyone working with Corynebacterium and related organisms in academia, biotechnology companies, and the pharmaceutical industry and is a recommended volume for all microbiology libraries. (caister.com)
  • SOS-induced spontaneous prophage induction in Corynebacterium glutamicum", Presented at the EMBL Heidelberg "New Approaches and Concepts in Microbiology", Heidelberg, Germany, 2013. (uni-bielefeld.de)
  • C. glutamicum has a circular chromosome and a plasmid. (kenyon.edu)
  • Plasmids were inserted into c. glutamicum , including an expression plasmid, that under certain conditions would create the P(3HB). (kenyon.edu)
  • Genetic evidence for a crtI2-1/2 encoded phytoene desaturase could not be obtained since plasmid-borne expression of crtI2-1/2 did not compensate for the lack of phytoene desaturase CrtI in C. glutamicum DeltacrtI. (uni-bielefeld.de)
  • Here, we analyze the expression of the C. glutamicum pqo gene, investigate the relevance of the PQO enzyme for growth and amino acid production, and perform phylogenetic studies. (asm.org)
  • Corynebacterium glutamicum grows on a variety of carbohydrates and organic acids. (asm.org)
  • Effect of carbon source availability and growth phase on expression of Corynebacterium glutamicum genes involved in the tricarboxylic acid cycle and glyoxylate bypass. (semanticscholar.org)
  • Corynebacterium glutamicum recently has been shown to possess pyruvate:quinone oxidoreductase (PQO), catalyzing the oxidative decarboxylation of pyruvate to acetate and CO 2 with a quinone as the electron acceptor. (asm.org)
  • Technologies for quantification, membrane enrichment and MS analysis tested in C. glutamicum might lead to higher coverage for membrane proteins or improve the accuracy and reliability of quantification based on isotope labeling. (caister.com)
  • Isolation of fully synthetic promoters for high-level gene expression in Corynebacterium glutamicum. (semanticscholar.org)
  • This book provides a comprehensive overview of current knowledge and research on C. glutamicum systems biology and biotechnological applications. (caister.com)
  • In summary, proteomics data are important for systems biology approaches in C. glutamicum . (caister.com)
  • 2007). Furthermore, within the last decade, C. glutamicum was applied in various production processes for fine chemicals, fuels and polymers. (caister.com)
  • C. glutamicum produces several useful compounds and enzymes. (kenyon.edu)
  • C. glutamicum cells are frequently confronted with excessive reactive oxygen species (ROS) production, triggering sudden changes of fermentative conditions in temperature, pH, osmotic pressure, or toxic compounds [ 4 - 5 ]. (portlandpress.com)
  • In contrast to Escherichia coli and Bacillus subtilis , which show distinct catabolite repression, C. glutamicum usually coutilizes the carbon sources present in the mixtures without showing diauxic growth. (asm.org)
  • A key development in recent years has been the engineering of C. glutamicum to utilize a broader spectrum of carbon sources (e.g. glycerol, galactose and pentose sugars) in addition to the traditional hexoses. (caister.com)