Cortodoxone
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Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
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Unctuous combustible substances that are liquid or easily liquefiable on warming, and are soluble in ether but insoluble in water. Such substances, depending on their origin, are classified as animal, mineral, or vegetable oils. Depending on their behavior on heating, they are volatile or fixed. (Dorland, 28th ed)
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Usually high-molecular-weight, straight-chain primary alcohols, but can also range from as few as 4 carbons, derived from natural fats and oils, including lauryl, stearyl, oleyl, and linoleyl alcohols. They are used in pharmaceuticals, cosmetics, detergents, plastics, and lube oils and in textile manufacture. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
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A plastic substance deposited by insects or obtained from plants. Waxes are esters of various fatty acids with higher, usually monohydric alcohols. The wax of pharmacy is principally yellow wax (beeswax), the material of which honeycomb is made. It consists chiefly of cerotic acid and myricin and is used in making ointments, cerates, etc. (Dorland, 27th ed)
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Adrenal Insufficiency
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One of two salivary glands in the neck, located in the space bound by the two bellies of the digastric muscle and the angle of the mandible. It discharges through the submandibular duct. The secretory units are predominantly serous although a few mucous alveoli, some with serous demilunes, occur. (Stedman, 25th ed)
Addison Disease
An adrenal disease characterized by the progressive destruction of the ADRENAL CORTEX, resulting in insufficient production of ALDOSTERONE and HYDROCORTISONE. Clinical symptoms include ANOREXIA; NAUSEA; WEIGHT LOSS; MUSCLE WEAKNESS; and HYPERPIGMENTATION of the SKIN due to increase in circulating levels of ACTH precursor hormone which stimulates MELANOCYTES.
A radioimmunoassay for human plasma corticosterone. (1/79)
A radioimmunoassay for human plasma corticosterone has been developed. Antiserum against corticosterone was produced in rabbits immunized with corticosterone-21-hemisuccinate conjugated to bovine serum albumin. The antiserum cross-reacted with progesterone, DOC and dehydrocorticosterone more than 20%. After the extraction with ether, and the separation by Sephadex LH-20 microcolumn chromatography, recovery was 51.2 +/- 12.1% in 50 assays. The mean coefficient of variation between assays was 7.7% and within assays was 8.6%. Human plasma corticosterone is measured readily by assaying aliquots of an ether extract of 0.05 to 0.1 ml of plasma after microcolumn chromatography. The mean plasma corticosterone concentration at 9 a.m. was 7.1 +/- 3.2 ng/ml in 45 normal subjects. Plasma corticosterone increased 5.2 times as much as basal values after ACTH injection, whereas radioimmunoassayed cortisol increased 2.4 times. On the other hand, plasma corticosterone decreased to 22.6% of basal values at four hours after 1 mg dexamethasone, whereas radioimmunoassayed cortisol decreased to 12.3% of basal values. (+info)Radioimmunoassay for 11-deoxycortisol using iodine-labeled tracer. (2/79)
A simple and sensitive radioimmunoassay for 11-deoxycortisol was developed. The antiserum produced in rabbits by immunizing with a complex of 11-deoxycortisol-3-oxime and bovine serum albumin (BSA) has little cross-reactivity with other endogenous steroids. The immunoassay procedure requires only one-step ethanol denaturation of binding proteins in plasma and extraction by an organic solvent can be omitted. Furthermore, use of 125I-labeled tracer significantly simplify the counting procedure. The method is sensitive enough to detect 1 microng/100 ml of 11-deoxycortisol. Plasma 11-deoxycortisol levels measured by this method after the administration of a single dose of metyrapone ranged from 5.0 to 19.2 microng/100 ml, whereas they were 0 to 4.0 microng/100 ml in hypopituitary patients. It is concluded that this simple method is useful for the routine assay of plasma 11-deoxycortisol as a parameter of the metyrapone tests. (+info)Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis. (3/79)
The specificity for 11-deoxycortisol (11-DOC) of a monoclonal antibody (mAb), designated SCET, was changed to specificity for cortisol (CS) by site-specific mutagenesis followed by random mutagenesis. The Fab form of SCET was expressed on the surface of a phage. During the first step, mutations were introduced at 14 amino acid positions in three complementarity-determining regions (CDRs) of the VH domain that seemed likely to form the steroid-binding pocket. A clone, DcC16, was isolated from the resultant library with multiple mutations and this clone was shown to have CS-binding activity but also to retain high 11-DOC-binding activity. During the second step, mutations were introduced randomly into the entire VH-coding region of the DcC16 clone by an error-prone polymerase chain reaction, and CS-specific mutant antibodies were selected in the presence of 11-DOC as a competitor. Three representative clones were analyzed with the BIAcore instrument, and each revealed a large increase in the binding constant for CS and a decrease in that for 11-DOC. Structural models, constructed by computer simulation, indicated the probable molecular basis for these changes in specificity. (+info)Apparent activities of 21-hydroxylase, 17alpha-hydroxylase and 17,20-lyase are impaired in adrenal incidentalomas. (4/79)
OBJECTIVE: An increased response of 17-hydroxyprogesterone to ACTH stimulation has been observed in adrenal incidentaloma and linked to an impairment of either 21-hydroxylase or of 11beta-hydroxylase activity. To analyse this question further, we investigated the steroidogenic pathways in a series of 17 adrenal incidentalomas. DESIGN AND PATIENTS: 17 patients (7 women, 10 men; mean age, 62 +/- 12 years) with non-histologically analyzed adrenal incidentalomas were prospectively evaluated. METHODS: The following variables were investigated: 24-h urinary methanephrines and free cortisol excretion; plasma levels of ACTH and dehydroepiandrosterone; overnight dexamethasone suppression test; 1-24 ACTH stimulation test with measurement of: cortisol, 11-deoxycortisol, 17-hydroxyprogesterone, aldosterone, 11-deoxycorticosterone, progesterone, 17-hydroxypregnenolone, Delta4-androstenedione, dehydroepiandrosterone and 21-deoxycortisol. RESULTS: Discordant features of subclinical hypercorticism were noted in one case. No patient had dehydroepiandrosterone sulfate levels in the normal range for his or her age. Peak 17-hydroxyprogesterone and peak 21-deoxycortisol disclosed impairment of 21-hydroxylase in 11 and 10 cases respectively. An increased 11-deoxycortisol/cortisol ratio identified reduced activity of 11beta-hydroxylase in 11 patients. Eight patients displayed features of mild 17,20-lyase impairment, which was related to 21-hydroxylase dysfunction. Whereas only 2 patients showed no enzyme modification, 9 displayed alterations of at least two pathways. CONCLUSION: In our hands, a combination of enzyme dysfunction was frequently observed. Shared biochemical mechanisms could explain combined 17,20-lyase and 21-hydroxylase alterations, whereas coexistence of 21-hydroxylase (particularly when based on peak 21-deoxycortisol) and 11beta-hydroxylase is more puzzling. (+info)Effects of insulin-like growth factor-I on in vitro final oocyte maturation and ovarian steroidogenesis in striped bass, Morone saxatilis. (5/79)
Recombinant human (rh) insulin-like growth factor-I (IGF-I) was more potent than rhIGF-II at inducing in vitro germinal vesicle breakdown (GVBD), a marker for resumption of meiosis, in oocytes of striped bass. Treatment of ovarian fragments containing oocytes in intact follicles with rhIGF-I increased concentrations of estradiol-17beta and maturation-inducing steroid (MIS) 17,20beta, 21-trihydoxy-4-pregnen-3-one (20beta-S) in the culture medium and decreased testosterone levels. The follicles were too immature for oocytes to complete GVBD in response to 20beta-S (MIS incompetent) or hCG. Addition of 20beta-S to cultures did not increase the percentage of oocytes completing GVBD in response to rhIGF-I or rhIGF-II. Bovine insulin was without effect on GVBD or steroid production. Incubation of MIS-competent follicles with actinomycin D, cyanoketone, trilostane, 1-heptanol, or 1-octanol had no effect on rhIGF-I-induced GVBD, but attenuated hCG-induced GVBD and 20beta-S production. Cycloheximide inhibited rhIGF-I-induced GVBD. Collectively, these observations indicate that IGF-I can induce GVBD via MIS- and transcription-independent pathways without coupled gap junctions between oocytes and granulosa cells or among granulosa cells, but requires protein synthesis to do so. An rhIGF-I analogue that does not bind IGF-binding proteins, des(1,3)IGF-I, was more potent than rhIGF-I in inducing GVBD, suggesting ovarian IGF-binding proteins may inhibit IGF-I action. (+info)Nonchromatographic radioimmunoassay of plasma 11-deoxycortisol, for use in the metyrapone test, with polyethylene glycol as the precipant. (6/79)
We have developed a simple, reliable radioimmunoassay for plasma 11-deoxycortisol. The method does not require chromatography but instead makes use of a simple extraction procedure which, in combination with the antibody characteristics, is highly specific for the metyrapone test. Polyethylene glycol was used to separate free and antibody-bound steroid. The smallest amount measurable is 15 pg (2.0 mug/liter of plasma). The method is shown to be precise and accurate. Intraassay precision of the method for two plasma pools was 26.7 plus or minus 2.5 mug/liter (CV equals 9.4%) and 61.2 plus or minus 3.7 mug/liter (CV equals 6.0%). The respective inter-assay precision was 27.0 plus or minus 1.7 mug/liter (CV equals 6.3%) and 59.9 plus or minus 2.3 mug/liter (CV equals 3.8%). The validity of the assay was further verified by evaluating the plasma 11-deoxycortisol response to metyrapone administration. The relative simplicity of the method and the commercial availability of all reagents, including antisera, makes this radioimmunoassay procedure practical for use in clinical laboratories. (+info)CYP11A1 stimulates the hydroxylase activity of CYP11B1 in mitochondria of recombinant yeast in vivo and in vitro. (7/79)
In mammals, hydrocortisone synthesis from cholesterol is catalyzed by a set of five specialized enzymes, four of them belonging to the superfamily of cytochrome P-450 monooxygenases. A recombinant yeast expression system was recently developed for the CYP11B1 (P45011beta) enzyme, which performs the 11beta hydroxylation of steroids such as 11-deoxycortisol into hydrocortisone, one of the three mitochondrial cytochrome P-450 proteins involved in steroidogenesis in mammals. This heterologous system was used to test the potential interaction between CYP11B1 and CYP11A1 (P450scc), the mitochondrial cytochrome P-450 enzyme responsible for the side chain cleaving of cholesterol. Recombinant CYP11B1 and CYP11A1 were targeted to Saccharomyces cerevisiae mitochondria using the yeast cytochrome oxidase subunit 6 mitochondrial presequence fused to the mature form of the two proteins. In yeast, the presence of CYP11A1 appears to improve 11beta hydroxylase activity of CYP11B1 in vivo and in vitro. Fractionation experiments indicate the presence of the two proteins in the same membrane fractions, i.e. inner membrane and contact sites of mitochondria. Thus, yeast mitochondria provide interesting insights to study some molecular and cellular aspects of mammalian steroid synthesis. In particular, recombinant yeast should permit a better understanding of the mechanism permitting the synthesis of steroids (sex steroids, mineralocorticoids and glucocorticoids) with a minimal set of enzymes at physiological level, thus avoiding disease states. (+info)Parallel radioimmunoassay for plasma cortisol and 11-deoxycortisol. (8/79)
We describe a direct, rapid, and specific procedure for the parallel radioimmunoassay for cortisol and 11-deoxycortisol in plasma. The plasma sample is used directly, after heat inactivation of the natural cortisol-binding protein. The radioimmunoassay utilizes antibodies generated in rabbits by steroids congugated at their 3-oxo position to thyroglobulin. Ammonium sulfate is used to separate bound and free steroids. Our cortisol antibody and an 11-deoxycortisol antibody obtained elsewhere cross reacted negligibly with each other or with other steroids that might be present in plasma. Radioimmunoassays were therefore developed for both steroids in only 1.25 mul of plasma. The intra- and inter-assay coefficients of variation for both steroids were less than 10%, with a sensitivyt of 4 mug/liter. Steroid values obtained by a competitive protein binding method were consistently higher than those of the present method, suggesting that the former is measuring total corticosteroids. This simple approach requires only 4 h for the specific measurement of both cortisol and 11-deoxycortisol in 20 samples of plasma. (+info)
11-Deoxycortisol - Wikipedia
11-Deoxycortisol, Serum -
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11-Deoxycortisol
... , also known as cortodoxone (INN) or cortexolone, as well as 17α,21-dihydroxyprogesterone or 17α,21- ...
Deoxycortisol
... may refer to: 11-Deoxycortisol (cortodoxone, cortexolone, Reichstein's Substance; 17α,21-dihydroxyprogesterone) ...
Cortifen
It is a hydrophobic chlorphenacyl nitrogen mustard ester of 11-deoxycortisol (cortodoxone). List of hormonal cytostatic ...
List of corticosteroids
... cortodoxone, cortexolone) = 17α,21-dihydroxypregn-4-ene-3,20-dione 11-Ketoprogesterone (11-oxoprogesterone; Ketogestin) = pregn ...
List of corticosteroid esters
... cortodoxone chlorphenacyl ester) Cortisone acetate Corticosterone acetate Corticosterone benzoate Cortodoxone acetate ...
List of drugs: Cm-Co
Cortoderm cortodoxone (INN) Cortone Cortril Cortrophin-Zinc Cortrosyn Corvert Coryphen Codeine Corzide cositecan (USAN, INN) ...
List of MeSH codes (D06)
... cortodoxone MeSH D06.472.040.585.478.392 - hydrocortisone MeSH D06.472.040.585.478.782 - tetrahydrocortisol MeSH D06.472. ...
Compound S
... may refer to: Cortodoxone Zidovudine, by trade name This disambiguation page lists articles associated with the ...
List of MeSH codes (D04)
... cortodoxone MeSH D04.808.745.745.654.339 - desoxycorticosterone MeSH D04.808.745.745.654.339.400 - 18- ...
Methylprednisolone
Cortodoxone (cortexolone, 11-deoxycortisol). *Desoxycortone (deoxycortone, cortexone, 11-deoxycorticosterone) *Desoxycortone ...
7α-Thiomethylspironolactone
11-Deoxycortisol (cortodoxone, cortexolone) *Cortifen (cortiphen, kortifen). *Cortodoxone acetate. *11β-Hydroxyprogesterone ...
Nicocortonide
11-Deoxycortisol (cortodoxone, cortexolone) *Cortifen (cortiphen, kortifen). *Cortodoxone acetate. *21-Deoxycortisol. *Δ7- ...
Cortisol
11-Deoxycortisol (cortodoxone, cortexolone) *Cortifen (cortiphen, kortifen). *Cortodoxone acetate. *21-Deoxycortisol. *Δ7- ...
Prorenoate potassium
11-Deoxycortisol (cortodoxone, cortexolone) *Cortifen (cortiphen, kortifen). *Cortodoxone acetate. *11β-Hydroxyprogesterone ...
Dicirenone
11-Deoxycortisol (cortodoxone, cortexolone) *Cortifen (cortiphen, kortifen). *Cortodoxone acetate. *11β-Hydroxyprogesterone ...
AZD-5423
11-Deoxycortisol (cortodoxone, cortexolone) *Cortifen (cortiphen, kortifen). *Cortodoxone acetate. *21-Deoxycortisol. *Δ7- ...
18-Deoxyaldosterone
11-Deoxycortisol (cortodoxone, cortexolone) *Cortifen (cortiphen, kortifen). *Cortodoxone acetate. *11β-Hydroxyprogesterone ...
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Cortodoxone/Deoxycortisol. *脱氢表雄酮. *硫酸脱氢表雄酮 ...
11-Deoxycortisol - Wikipedia
11-deoxycortisol (CHEBI:28324)
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CYP260B1 performs the regio- and stereoselective hydroxylation of the glucocorticoid cortodoxone (RSS) to produce 6 -OH-RSS. ... 0 (Blood Glucose); 0 (Plant Oils); 0 (Sulfates); Q9L0O73W7L (Coconut Oil); W980KJ009P (Corticosterone); WDT5SLP0HQ (Cortodoxone ... 0 (Bacterial Proteins); 0 (Recombinant Proteins); EC 1.14.- (Steroid Hydroxylases); WDT5SLP0HQ (Cortodoxone). ...
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Csengeri, D., Sprünker, N-A., Di Castelnuovo, A., Niiranen, T., Vishram-Nielsen, J. K., Costanzo, S., Söderberg, S., Jensen, S. M., Vartiainen, E., Donati, M. B., Magnussen, C., Camen, S., Gianfagna, F., Løchen, M-L., Kee, F., Kontto, J., Mathiesen, E. B., Koenig, W., Stefan, B., de Gaetano, G. & 6 flere, Jørgensen, T., Kuulasmaa, K., Zeller, T., Salomaa, V., Iacoviello, L. & Schnabel, R. B., 21 mar. 2021, I: European Heart Journal. 42, 12, s. 1170-1177 8 s.. Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review ...
Deoxycortisol - Wikipedia
Short and long-term responses to metyrapone in the medical management of 91 patients with Cushing's syndrome
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Tzakis, A. G., Kato, T., Levi, D. M., DeFaria, W., Selvaggi, G., Weppler, D., Nishida, S., Moon, J., Madariaga, J. R., David, A. I., Gaynor, J. J., Thompson, J., Hernandez, E., Martinez, E., Cantwell, G. P., Augenstein, J. S., Gyamfi, A., Pretto, E. A., Dowdy, L., Tryphonopoulos, P. & 7 others, Ruiz, P., Klintmalm, G. B., Starzl, T. E., Abu-Elmagd, K. M., Grant, D., Najarian, J. S. & Trunkey, D. D., Oct 1 2005, In : Annals of surgery. 242, 4, p. 480-493 14 p.. Research output: Contribution to journal › Article ...
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Hathout, Y., Brody, E., Clemens, P. R., Cripe, L., DeLisle, R. K., Furlong, P., Gordish-Dressman, H., Hache, L., Henricson, E. K., Hoffman, E. P., Kobayashi, Y. M., Lorts, A., Mah, J. K., McDonald, C. M., Mehler, B., Nelson, S., Nikrad, M., Singer, B., Steele, F., Sterling, D. & 3 othersSweeney, H. L., Williams, S. & Gold, L., Jun 9 2015, In : Proceedings of the National Academy of Sciences of the United States of America. 112, 23, p. 7153-7158 6 p.. Research output: Contribution to journal › Article ...
Cortexolone4
- 11-Deoxycortisol , also known as cortodoxone ( INN ) or cortexolone , as well as 17α,21-dihydroxyprogesterone or 17α,21-dihydroxypregn-4-ene-3,20-dione , [1] is a glucocorticoid steroid hormone . (wikipedia.org)
- Cortisone Cortisone acetate Cortodoxone cortexolone, deoxycortisol Desoxycortone deoxycortone, cortexone, deoxycorticosterone Desoxycortone esters Hydrocortisone cortisol Hydrocortisone esters Prebediolone acetate Pregnenolone Pregnenolone acetate Pregnenolone succinate. (buynaltrexoneonline.com)
- Glucocorticoids and antiglucocorticoids H Cortisone Cortisone acetate Cortodoxone cortexolone, deoxycortisol Desoxycortone deoxycortone, cortexone, deoxycorticosterone Desoxycortone esters Hydrocortisone cortisol Hydrocortisone esters Prebediolone acetate Pregnenolone Pregnenolone acetate Pregnenolone succinate. (cialispreisvergleich.top)
- Retrieved July 31, Cortisone Cortisone acetate Cortodoxone cortexolone, deoxycortisol Desoxycortone deoxycortone, cortexone, deoxycorticosterone Desoxycortone esters Hydrocortisone cortisol Hydrocortisone esters Prebediolone [8] and stop the development. (zackariasson.com)
Hydrocortisone1
- Cortodoxone is a glucocorticoid steroid hormone that can be oxygenated to cortisol (Hydrocortisone). (csnpharm.cn)
Deoxycortisol2
- An internal standard for 11-deoxycortisol, also known as cortodoxone. (fishersci.com)
- It is a hydrophobic chlorphenacyl nitrogen mustard ester of 11-deoxycortisol (cortodoxone). (wikipedia.org)
Steroid1
- Dimethisterone, also known as 6α,21-dimethylethisterone or as 6α,21-dimethyl-17α-ethynyltestosterone, as well as 17α-ethynyl-6α,21-dimethylandrost-4-en-17β-ol-3-one or as 6α,21-dimethyl-17β-hydroxy-17α-pregn-4-en-20-yn-3-one, is a synthetic androstane steroid and a derivative of testosterone . (readtiger.com)