Corticoviridae
The complete genome sequence of PM2, the first lipid-containing bacterial virus To Be isolated. (1/17)
Bacteriophage PM2 was isolated from the Pacific Ocean off the coast of Chile in the late 1960s. It was a new virus type, later classified as Corticoviridae, and also the first bacterial virus for which it was demonstrated that lipids are part of the virion structure. Here we report the determination and analysis of the 10, 079-bp circular dsDNA genome sequence. Noteworthy discoveries are the replication initiation system, which is related to the rolling circle mechanism described for phages such as φX174 and P2, and a 1.2-kb sequence that is similar to the maintenance region of a plasmid found in a marine Pseudoalteromonas sp. strain A28. (+info)Purification and protein composition of PM2, the first lipid-containing bacterial virus to be isolated. (2/17)
The marine, icosahedral bacteriophage PM2 was isolated in the late 1960s. It was the first phage for which lipids were firmly demonstrated to be part of the virion structure and it has been classified as the type organism of the Corticoviridae family. The host, Pseudoalteromonas espejiana BAL-31, belongs to a common group of marine bacteria. We developed a purification method producing virions with specific infectivity approximately as high as that of the lipid-containing phages PRD1 and φ6. The sensitivity of the virus to normally used purification media such as those containing sucrose is demonstrated. We also present an alternative host, a pseudoalteromonad, that allows enhanced purification of the virus under reduced salt conditions. We show, using N-terminal amino acid sequencing and comparison with the genomic sequence, that there are at least eight structural proteins in the infectious virus. (+info)A conserved genetic module that encodes the major virion components in both the coliphage T4 and the marine cyanophage S-PM2. (3/17)
Sequence analysis of a 10-kb region of the genome of the marine cyanomyovirus S-PM2 reveals a homology to coliphage T4 that extends as a contiguous block from gene (g)18 to g23. The order of the S-PM2 genes in this region is similar to that of T4, but there are insertions and deletions of small ORFs of unknown function. In T4, g18 codes for the tail sheath, g19, the tail tube, g20, the head portal protein, g21, the prohead core protein, g22, a scaffolding protein, and g23, the major capsid protein. Thus, the entire module that determines the structural components of the phage head and contractile tail is conserved between T4 and this cyanophage. The significant differences in the morphology of these phages must reflect the considerable divergence of the amino acid sequence of their homologous virion proteins, which uniformly exceeds 50%. We suggest that their enormous diversity in the sea could be a result of genetic shuffling between disparate phages mediated by such commonly shared modules. These conserved sequences could facilitate genetic exchange by providing partially homologous substrates for recombination between otherwise divergent phage genomes. Such a mechanism would thus expand the pool of phage genes accessible by recombination to all those phages that share common modules. (+info)Bacteriophage PM2 has a protein capsid surrounding a spherical proteinaceous lipid core. (4/17)
The marine double-stranded DNA (dsDNA) bacteriophage PM2, studied since 1968, is the type organism of the family Corticoviridae, infecting two gram-negative Pseudoalteromonas species. The virion contains a membrane underneath an icosahedral protein capsid composed of two structural proteins. The purified major capsid protein, P2, appears as a trimer, and the receptor binding protein, P1, appears as a monomer. The C-terminal part of P1 is distal and is responsible for receptor binding activity. The rest of the structural proteins are associated with the internal phospholipid membrane enclosing the viral genome. This internal particle is designated the lipid core. The overall structural organization of phage PM2 resembles that of dsDNA bacteriophage PRD1, the type organism of the family TECTIVIRIDAE: (+info)Transcription of bacteriophage PM2 involves phage-encoded regulators of heterologous origin. (5/17)
Bacteriophage PM2 is the only described member of the Corticoviridae family. It is an icosahedral dsDNA virus with a membrane residing underneath the protein coat. PM2 infects some gram-negative Pseudoalteromonas spp. In the present study, we mapped the viral promoters and showed that the PM2 genome consists of three operons. Four new virus genes were assigned based on their function in transcription. Proteins P15 and P16 are shown to repress early transcription, and proteins P13 and P14 are shown to activate late transcription events. The early regulatory region, containing genes for proteins P15 and P16, as well as the newly identified early promoter region in PM2, has significant sequence similarity with the Pseudoalteromonas pAS28 plasmid. P14, the transcription activator for the structural genes, has a zinc finger motif homologous to archaeal and eukaryotic TFIIS-type regulatory factors. (+info)Penetration of membrane-containing double-stranded-DNA bacteriophage PM2 into Pseudoalteromonas hosts. (6/17)
The icosahedral bacteriophage PM2 has a circular double-stranded DNA (dsDNA) genome and an internal lipid membrane. It is the only representative of the Corticoviridae family. How the circular supercoiled genome residing inside the viral membrane is translocated into the gram-negative marine Pseudoalteromonas host has been an intriguing question. Here we demonstrate that after binding of the virus to an abundant cell surface receptor, the protein coat is most probably dissociated. During the infection process, the host cell outer membrane becomes transiently permeable to lipophilic gramicidin D molecules proposing fusion with the viral membrane. One of the components of the internal viral lipid core particle is the integral membrane protein P7, with muralytic activity that apparently aids the process of peptidoglycan penetration. Entry of the virion also causes a limited depolarization of the cytoplasmic membrane. These phenomena differ considerably from those observed in the entry process of bacteriophage PRD1, a dsDNA virus, which uses its internal membrane to make a cell envelope-penetrating tubular structure. (+info)Biochemical quantitation of PM2 phage DNA as a substrate for endonuclease assay. (7/17)
Bacteriophage PM2 has a closed circular form of double stranded DNA as a genome. This DNA from the phage is a useful source for nick-circle endonuclease assay in the fmol range. Due to difficulties in the maintenance of viral infectivity, storage conditions of the phage should be considered for the purification of PM2 DNA. The proper condition for a short-term storage of less than 2 months is to keep the PM2 phage at 4 degrees C; whereas the proper condition for a long-term storage of the PM2 phage for over 2 months is to keep it under liquid nitrogen in 7.5% glycerol. The optimal conditions for a high yield of phage progeny were also considered with the goal to achieve a successful PM2 DNA preparation. A MOI(Multiplicity Of Infection) of 0.03, in which the OD600 of the host bacteria was between 0.3 and 0.5, turned out to be optimal for the mass production of PM2 phage with a burst size of about 214. Considerations of PM2 genome size, and the concentrations and radiospecific activities of purified PM2 DNA, are required to measure the endonuclease activity in the fmol range. This study reports the proper quantitation of radioactivity and the yield of purified DNA based on these conditions. (+info)Preliminary crystallographic analysis of the major capsid protein P2 of the lipid-containing bacteriophage PM2. (8/17)
PM2 (Corticoviridae) is a dsDNA bacteriophage which contains a lipid membrane beneath its icosahedral capsid. In this respect it resembles bacteriophage PRD1 (Tectiviridae), although it is not known whether the similarity extends to the detailed molecular architecture of the virus, for instance the fold of the major coat protein P2. Structural analysis of PM2 has been initiated and virus-derived P2 has been crystallized by sitting-nanodrop vapour diffusion. Crystals of P2 have been obtained in space group P2(1)2(1)2, with two trimers in the asymmetric unit and unit-cell parameters a = 171.1, b = 78.7, c = 130.1 A. The crystals diffract to 4 A resolution at the ESRF BM14 beamline (Grenoble, France) and the orientation of the non-crystallographic threefold axes, the spatial relationship between the two trimers and the packing of the trimers within the unit cell have been determined. The trimers form tightly packed layers consistent with the crystal morphology, possibly recapitulating aspects of the arrangement of subunits in the virus. (+info)
Corticoviridae - Corticoviridae - dsDNA Viruses - International Committee on Taxonomy of Viruses (ICTV)
Corticoviridae - Corticoviridae - dsDNA Viruses - International Committee on Taxonomy of Viruses (ICTV)
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Corticovirus
Corticoviridae. NCBI Taxonomy. Kiveld, H.M., Kalkkinen, N. and Bamford, D.H. (2002). Bacteriophage PM2 has a protein capsid ... "Corticoviridae". ICTV Online (10th) Report. "Viral Zone". ExPASy. Retrieved 15 June 2015. Krupovic M, Bamford DH (2007). " ... Corticovirus is a genus of viruses in the family Corticoviridae. Corticoviruses are bacteriophages; that is, their natural ... ICTV Online(10th) Report: Corticoviridae Viralzone: Corticovirus (Articles with short description, Short description is ...
Marine viruses
These non-tailed phages also infect marine bacteria, and include the families Corticoviridae,Inoviridae,Microviridae and ... However, viruses belonging to families Corticoviridae, Inoviridae and Microviridae are also known to infect diverse marine ...
Marine life
Bacteriophages belonging to the families Corticoviridae, Inoviridae and Microviridae are also known to infect diverse marine ...
Marine microorganisms
Phages belonging to the families Corticoviridae,Inoviridae,Microviridae, and Autolykiviridae are also known to infect diverse ...
Archaeal virus
... which is related to bacterial virus families Tectiviridae and Corticoviridae and many eukaryotic virus families such as ...
Cystovirus
No other bacteriophage has any lipid in its outer coat, though the Tectiviridae and the Corticoviridae have lipids within their ...
Varidnaviria
... in the case of the family Corticoviridae. An exception is the order Halopanivirales, whose members do not encode any ...
List of virus families and subfamilies
Caulimoviridae Chaseviridae Chrysoviridae Chuviridae Circoviridae Clavaviridae Closteroviridae Coronaviridae Corticoviridae ...
JYX - ICTV Virus Taxonomy Profile : Finnlakeviridae
Code System Concept
DeCS
Corticoviridae - Preferred Concept UI. M0028649. Scope note. A family of icosahedral, lipid-containing, non-enveloped ... Corticoviridae. Scope note:. Familia de bacteriófagos icosaédrocos que contienen lípidos y carecen de envoltura; contiene sólo ... Corticoviridae Entry term(s):. Alteromonas phage PM2. Bacteriophage PM2. Bacteriophage, PM2. Bacteriophages, PM2. Corticovirus ...
DeCS 2016 - June 12, 2016 version
Virus
Toulouse, Marie-Josée
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Corticoviridae ~ ViralZone
TREE NUMBER DESCRIPTOR
MeSH Browser
Corticoviridae Preferred Term Term UI T057308. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1997). ... The sole species in the genus Corticovirus, family CORTICOVIRIDAE.. Terms. Alteromonas phage PM2 Preferred Term Term UI T431054 ... Corticoviridae Preferred Concept UI. M0028649. Registry Number. txid10659. Related Numbers. txid10660. txid10661. Scope Note. A ... Corticoviridae. Tree Number(s). B04.123.210. Unique ID. D019240. RDF Unique Identifier. http://id.nlm.nih.gov/mesh/D019240 ...
MeSH Browser
Corticoviridae Preferred Term Term UI T057308. Date01/01/1999. LexicalTag NON. ThesaurusID NLM (1997). ... The sole species in the genus Corticovirus, family CORTICOVIRIDAE.. Terms. Alteromonas phage PM2 Preferred Term Term UI T431054 ... Corticoviridae Preferred Concept UI. M0028649. Registry Number. txid10659. Related Numbers. txid10660. txid10661. Scope Note. A ... Corticoviridae. Tree Number(s). B04.123.210. Unique ID. D019240. RDF Unique Identifier. http://id.nlm.nih.gov/mesh/D019240 ...
Code System Concept
BIOSIS Previews 說明
c33c
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Complete genomes: Corticovirus
Meta-Genome Retrieval
Herpesviridae<...
The nucleotide or predicted amino acid sequences of the subfamily members form a distinct lineage within the family. Certain genes may be unique to members of the subfamily. These include BNRF-1, BTRF-1 and BRLF-1 of HHV-4 (the corresponding ORFs of saimiriine herpesvirus 2 are ORFs 3, 23 and 50, respectively). Many members of the subfamily infect lymphocytes in vitro, and "carrier" cultures can be established in which a minority of cells is productively infected. Latent infection in vivo occurs in lymphocytes or lymphoid tissue. Acute infection is frequently associated with lymphoproliferative disorders and many members of the subfamily are associated with malignancies of lymphoid and non-lymphoid origin.. ...
Cortaillod (Neuchâtel) | Article about Cortaillod (Neuchâtel) by The Free Dictionary
TERM
Phage1
- The virion organization (pseudo T=21 dextro) and major capsid protein fold (double-β-barrel) resemble those of Pseudoalteromonas phage PM2 (family Corticoviridae), which has a double-stranded DNA genome. (jyu.fi)
Family1
- The sole species in the genus Corticovirus, family CORTICOVIRIDAE. (bvsalud.org)
Corticovirus1
- The sole species in the genus Corticovirus, family CORTICOVIRIDAE . (nih.gov)