Cyclic TETRAPYRROLES based on the corrin skeleton.
A cobalt-containing coordination compound produced by intestinal micro-organisms and found also in soil and water. Higher plants do not concentrate vitamin B 12 from the soil and so are a poor source of the substance as compared with animal tissues. INTRINSIC FACTOR is important for the assimilation of vitamin B 12.
A group of carrier proteins which bind with VITAMIN B12 in the BLOOD and aid in its transport. Transcobalamin I migrates electrophoretically as a beta-globulin, while transcobalamins II and III migrate as alpha-globulins.
A trace element that is a component of vitamin B12. It has the atomic symbol Co, atomic number 27, and atomic weight 58.93. It is used in nuclear weapons, alloys, and pigments. Deficiency in animals leads to anemia; its excess in humans can lead to erythrocytosis.
A chlorinated hydrocarbon used as an industrial solvent and cooling liquid in electrical transformers. It is a potential carcinogen.
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.

A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane. (1/71)

Methylobacterium sp. strain CM4, an aerobic methylotrophic alpha-proteobacterium, is able to grow with chloromethane as a carbon and energy source. Mutants of this strain that still grew with methanol, methylamine, or formate, but were unable to grow with chloromethane, were previously obtained by miniTn5 mutagenesis. The transposon insertion sites in six of these mutants mapped to two distinct DNA fragments. The sequences of these fragments, which extended over more than 17 kb, were determined. Sequence analysis, mutant properties, and measurements of enzyme activity in cell-free extracts allowed the definition of a multistep pathway for the conversion of chloromethane to formate. The methyl group of chloromethane is first transferred by the protein CmuA (cmu: chloromethane utilization) to a corrinoid protein, from where it is transferred to H4folate by CmuB. Both CmuA and CmuB display sequence similarity to methyltransferases of methanogenic archaea. In its C-terminal part, CmuA is also very similar to corrinoid-binding proteins, indicating that it is a bifunctional protein consisting of two domains that are expressed as separate polypeptides in methyl transfer systems of methanogens. The methyl group derived from chloromethane is then processed by means of pterine-linked intermediates to formate by a pathway that appears to be distinct from those already described in Methylobacterium. Remarkable features of this pathway for the catabolism of chloromethane thus include the involvement of a corrinoid-dependent methyltransferase system for dehalogenation in an aerobe and a set of enzymes specifically involved in funneling the C1 moiety derived from chloromethane into central metabolism.  (+info)

Methanol:coenzyme M methyltransferase from Methanosarcina barkeri -- substitution of the corrinoid harbouring subunit MtaC by free cob(I)alamin. (2/71)

Methyl-coenzyme M formation from coenzyme M and methanol in Methanosarcina barkeri is catalysed by an enzyme system composed of three polypeptides MtaA, MtaB and MtaC, the latter of which harbours a corrinoid prosthetic group. We report here that MtaC can be substituted by free cob(I)alamin which is methylated with methanol in an MtaB-catalysed reaction and demethylated with coenzyme M in an MtaA-catalysed reaction. Methyl transfer from methanol to coenzyme M was found to proceed at a relatively high specific activity at micromolar concentrations of cob(I)alamin. This finding was surprising because the methylation of cob(I)alamin catalysed by MtaB alone and the demethylation of methylcob(III)alamin catalysed by MtaA alone exhibit apparent Km for cob(I)alamin and methylcob(III)alamin of above 1 mm. A possible explanation is that MtaA positively affects the MtaB catalytic efficiency and vice versa by decreasing the apparent Km for their corrinoid substrates. Activation of MtaA by MtaB was methanol-dependent. In the assay for methanol:coenzyme M methyltransferase activity cob(I)alamin could be substituted by cob(I)inamide which is devoid of the nucleotide loop. Substitution was, however, only possible when the assays were supplemented with imidazole: approximately 1 mm imidazole being required for half-maximal activity. Methylation of cob(I)inamide with methanol was found to be dependent on imidazole but not on the demethylation of methylcob(III)inamide with coenzyme M. The demethylation reaction was even inhibited by imidazole. The structure and catalytic mechanism of the MtaABC complex are compared with the cobalamin-dependent methionine synthase.  (+info)

Native corrinoids from Clostridium cochlearium are adeninylcobamides: spectroscopic analysis and identification of pseudovitamin B(12) and factor A. (3/71)

The corrinoids from the obligate anaerobe Clostridium cochlearium were extracted as a mixture of Co(beta)-cyano derivatives. From 50 g of frozen cells, approximately 2 mg (1.5 micromol) of B(12) derivatives was obtained as a crystalline sample. Analysis of the corrinoid sample of C. cochlearium by a combination of high-pressure liquid chromatography and UV-Vis absorbance spectroscopy revealed the presence of three cyano corrinoids in a ratio of about 3:1:1. The spectroscopic data acquired for the sample indicated the main components to be pseudovitamin B(12) (Co(beta)-cyano-7"-adeninylcobamide) (60%) and factor A (Co(beta)-cyano-7"-[2-methyl]adeninylcobamide) (20%). Authentic pseudovitamin B(12) was prepared by guided biosynthesis from cobinamide and adenine. Both pseudovitamin B(12) and its homologue, factor A, were subjected to complete spectroscopic analysis by UV-Vis, circular dichroism, mass spectrometry, and by one- and two-dimensional (1)H, (13)C-, and (15)N nuclear magnetic resonance (NMR) spectroscopy. The third component was indicated by the mass spectra to be an isomer of factor A and is likely (according to NMR) to be 7"-[N(6)-methyl]-adeninylcobamide, a previously unknown corrinoid. C. cochlearium thus biosynthesizes as its native "complete" B(12) cofactors the 7"-adeninylcobamides and two homologous corrinoids, in which the nucleotide base is a methylated adenine.  (+info)

The enigma of cobalamin (Vitamin B12) biosynthesis in Porphyromonas gingivalis. Identification and characterization of a functional corrin pathway. (4/71)

The ability of Porphyromonas gingivalis to biosynthesize tetrapyrroles de novo has been investigated. Extracts of the bacterium do not possess activity for 5- aminolevulinic-acid dehydratase or porphobilinogen deaminase, two key enzymes involved in the synthesis of uroporphyrinogen III. Similarly, it was not possible to detect any genetic evidence for these early enzymes with the use of degenerate polymerase chain reaction. However, the bacterium does appear to harbor some of the enzymes for cobalamin biosynthesis since cobyric acid, a pathway intermediate, was converted into cobinamide. Furthermore, degenerate polymerase chain reaction with primers to cbiP, which encodes cobyric-acid synthase, produced a fragment with a high degree of identity to Salmonella typhimurium cbiP. Indeed, the recently released genome sequence data confirmed the presence of cbiP together with 14 other genes of the cobalamin pathway. A number of these genes were cloned and functionally characterized. Although P. gingivalis harbors all the genes necessary to convert precorrin-2 into cobalamin, it is missing the genes for the synthesis of precorrin-2. Either the organism has a novel pathway for the synthesis of precorrin-2, or more likely, it has lost this early part of the pathway. The remainder of the pathway may be being maintained to act as a salvage route for corrin synthesis.  (+info)

The Na(+)-translocating methyltransferase complex from methanogenic archaea. (5/71)

Methanogenic archaea are dependent on sodium ions for methane formation. A sodium ion-dependent step has been shown to be methyl transfer from N(5)-methyltetrahydromethanopterin to coenzyme M. This exergonic reaction (DeltaG degrees '=-30 kJ/mol) is catalyzed by a Na(+)-translocating membrane-associated multienzyme complex composed of eight different subunits, MtrA-H. Subunit MtrA harbors a cob(I)amide prosthetic group which is methylated and demethylated in the catalytic cycle, demethylation being sodium ion-dependent. Based on the finding that in the cob(II)amide oxidation state the corrinoid is bound in a base-off/His-on configuration it is proposed that methyl transfer from MtrA to coenzyme M is associated with a conformational change of the protein and that this change drives the electrogenic translocation of the sodium ions.  (+info)

Identification and in vivo characterization of PpaA, a regulator of photosystem formation in Rhodobacter sphaeroides. (6/71)

A regulatory protein, PpaA, involved in photosystem formation in the anoxygenic phototrophic proteobacterium Rhodobacter sphaeroides has been identified and characterized in vivo. Based on the phenotypes of cells expressing the ppaA gene in extra copy and on the phenotype of the ppaA null mutant, it was concluded that PpaA activates photopigment production and puc operon expression under aerobic conditions. This is in contrast to the function of the PpaA homologue from Rhodobacter capsulatus, AerR, which acts as a repressor under aerobic conditions [Dong, C., Elsen, S., Swem, L. R. & Bauer, C. E. (2002). J Bacteriol 184, 2805-2814]. The expression of the ppaA gene increases several-fold in response to a decrease in oxygen tension, suggesting that the PpaA protein is active under conditions of low or no oxygen. However, no discernible phenotype of a ppaA null mutant was observed under anaerobic conditions tested thus far. The photosystem gene repressor PpsR mediates repression of ppaA gene expression under aerobic conditions. Sequence analysis of PpaA homologues from several anoxygenic phototrophic bacteria revealed a putative corrinoid-binding domain. It is suggested that PpaA binds a corrinoid cofactor and the availability or structure of this cofactor affects PpaA activity.  (+info)

ABC transporter for corrinoids in Halobacterium sp. strain NRC-1. (7/71)

We report evidence for the existence of a putative ABC transporter for corrinoid utilization in the extremely halophilic archaeon Halobacterium sp. strain NRC-1. Results from genetic and nutritional analyses of Halobacterium showed that mutants with lesions in open reading frames (ORFs) Vng1370G, Vng1371Gm, and Vng1369G required a 10(5)-fold higher concentration of cobalamin for growth than the wild-type or parent strain. The data support the conclusion that these ORFs encode orthologs of the bacterial cobalamin ABC transporter permease (btuC; Vng1370G), ATPase (btuD; Vng1371Gm), and substrate-binding protein (btuF; Vng1369G) components. Mutations in the Vng1370G, Vng1371Gm, and Vng1369G genes were epistatic, consistent with the hypothesis that their products work together to accomplish the same function. Extracts of btuF mutant strains grown in the presence of cobalamin did not contain any cobalamin molecules detectable by a sensitive bioassay, whereas btuCD mutant strain extracts did. The data are consistent with the hypothesis that the BtuF protein is exported to the extracellular side of the cell membrane, where it can bind cobalamin in the absence of BtuC and BtuD. Our data also provide evidence for the regulation of corrinoid transport and biosynthesis. Halobacterium synthesized cobalamin in a chemically defined medium lacking corrinoid precursors. To the best of our knowledge, this is the first genetic analysis of an archaeal corrinoid transport system.  (+info)

Evidence for the involvement of corrinoids and factor F430 in the reductive dechlorination of 1,2-dichloroethane by Methanosarcina barkeri. (8/71)

Cobalamin and the native and diepimeric forms of factor F430 catalyzed the reductive dechlorination of 1,2-dichloroethane (1,2-DCA) to ethylene or chloroethane (CA) in a buffer with Ti(III) citrate as the electron donor. Ethylene was the major product in the cobalamin-catalyzed transformation, and the ratio of ethylene to CA formed was 25:1. Native F430 and 12,13-di-epi-F430 produced ethylene and CA in ratios of about 2:1 and 1:1, respectively. Cobalamin dechlorinated 1,2-DCA much faster than did factor F430. Dechlorination rates by all three catalysts showed a distinct pH dependence, correlated in a linear manner with the catalyst concentration and doubled with a temperature increase of 10 degrees C. Crude and boiled cell extracts of Methanosarcina barkeri also dechlorinated 1,2-DCA to ethylene and CA with Ti(III) citrate as the reductant. The catalytic components in boiled extracts were heat and oxygen stable and had low molecular masses. Fractionation of boiled extracts by a hydrophobic interaction column revealed that part of the dechlorinating components had a hydrophilic and part had a hydrophobic character. These chemical properties of the dechlorinating components and spectral analysis of boiled extracts indicated that corrinoids or factor F430 was responsible for the dechlorinations. The ratios of 3:1 to 7:1 of ethylene and CA formed by cell extracts suggested that both cofactors were concomitantly active.  (+info)

Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to estimate the distribution of corrinoids - the cobalamins hydroxocobalamin (OH-cbl), methylcobalamin (me-cbl) and 5-deoxyadenosylcobalamin (ado-cbl), and cobalamin analogues - in these tissues. Samples were taken from either cobalt-deficient or cobalt-replete ewes, and ruminant and pre-ruminant lambs. In liver, ado-cbl predominated, followed by analogues, OH-cbl and me-cbl. Supplementation with either cobalt (ruminant) or vitamin B12 injections (pre-ruminant) increased the amount of ado-cbl and decreased analogues. In blood, OH-cbl predominated, followed by ado-cbl, analogues and me-cbl, respectively. In small intestinal fluid, the distribution from largest to smallest percentage was analogues, ado-cbl, OH-cbl and me-cbl. In faeces, analogues constituted the greatest proportion,
Background Corrinoids are an essential cofactor of reductive dehalogenases, the key enzymes of organohalide respiration (OHR). Dehalobacter restrictus is an obligate OHR bacterium (OHRB) able to conserve energy with tetrachloroethene, but is unable to de novo synthesize corrinoids. Genome analysis of D. restrictus strain PER-K23 however revealed the presence of the complete corrinoid biosynthesis pathway. Objectives The aim of the present study is to understand the corrinoid metabolism of D. restrictus strain PER-K23 at the level of biosynthesis, regulation and transport and to compare it to contrasting situations in other Dehalobacter strains. Methods Genome analysis was performed with standard bioinformatic tools. Both transcriptomic and proteomic approaches were applied on D. restrictus strain PER-K23 cells cultivated in media with alternative corrinoid conditions. Gene expression was further addressed using targeted reverse transcription and quantitative PCR. Growth and dechlorination of D. ...
TY - JOUR. T1 - One pathway can incorporate either adenine or dimethylbenzimidazole as an α-axial ligand of B12 cofactors in Salmonella enterica. AU - Anderson, Peter J.. AU - Lango, Jozsef. AU - Carkeet, Colleen. AU - Britten, Audrey. AU - Kräutler, Bernhard. AU - Hammock, Bruce D.. AU - Roth, John R.. PY - 2008/2. Y1 - 2008/2. N2 - Corrinoid (vitamin B12-like) cofactors contain various α-axial ligands, including 5,6-dimethylbenzimidazole (DMB) or adenine. The bacterium Salmonella enterica produces the corrin ring only under anaerobic conditions, but it can form complete corrinoids aerobically by importing an incomplete corrinoid, such as cobinamide (Cbi), and adding appropriate α- and β-axial ligands. Under aerobic conditions, S. enterica performs the corrinoid-dependent degradation of ethanolamine if given vitamin B12, but it can make B12 from exogenous Cbi only if DMB is also provided. Mutants isolated for their ability to degrade ethanolamine without added DMB converted Cbi to ...
BACKGROUNDMethyltetrahydrofolate, corrinoid iron-sulfur protein methyltransferase (MeTr), catalyzes a key step in the Wood-Ljungdahl pathway of carbon dioxide fixation. It transfers the N5-methyl group from methyltetrahydrofolate (CH3-H4folate) to a cob(I)amide center in another protein, the corrinoid iron-sulfur protein. MeTr is a member of a family of proteins that includes methionine synthase and methanogenic enzymes that activate the methyl group of methyltetra-hydromethano(or -sarcino)pterin. We report the first structure of a protein in this family.RESULTSWe determined the crystal structure of MeTr from Clostridium thermoaceticum at 2.2 A resolution using multiwavelength anomalous diffraction methods. The overall architecture presents a new functional class of the versatile triose phosphate isomerase (TIM) barrel fold. The MeTr tertiary structure is surprisingly similar to the crystal structures of dihydropteroate synthetases despite sharing less than 20% sequence identity. This homology ...
Vitamin B12 is the largest and most complex of all vitamins. Vitamin B12 is the generic name for a specific group of cobalt-containing corrinoids from biological activity in humans. What is interesting is the only known metabolite contains cobalt, which gives a water-soluble vitamin its red color. This group of corrinoids is also known as cobalamin. The principal cobalamins in humans and animals are hydroxocobalamin, adenosylcobalamin and methylcobalamin, the latter two are active coenzyme forms. Cyanocobalamin is a form of vitamin B12, which is widely used clinically because of the availability and stability. Converted into active agents in the body ...
Vitamin B12 is the largest and most complex of all vitamins. Vitamin B12 is the generic name for a specific group of cobalt-containing corrinoids from biological activity in humans. What is interesting is the only known metabolite contains cobalt, which gives a water-soluble vitamin its red color. This group of corrinoids is also known as cobalamin. The principal cobalamins in humans and animals are hydroxocobalamin, adenosylcobalamin and methylcobalamin, the latter two are active coenzyme forms. Cyanocobalamin is a form of vitamin B12, which is widely used clinically because of the availability and stability. Converted into active agents in the body ...
RN [1] RM 96134964 RT Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila. RA Maupin-Furlow J, Ferry JG RL J Bacteriol 1996 Jan;178(2):340-6 RN [2] RT Cloning and expression of the gene cluster encoding key proteins involved in acetyl-CoA synthesis in Clostridium thermoaceticum: CO dehydrogenase, the corrinoid/Fe-S protein, and methyltransferase. RA Roberts DL, James-Hagstrom JE, Garvin DK, Gorst CM, Runquist JA, Baur JR, Haase FC, Ragsdale SW RL Proc Natl Acad Sci U S A 1989 Jan;86(1):32-6 SE TIGR GA hmmls AL clustalw, belvu DR HAMAP; MF_01135; 9 of ...
The total synthesis of the complex biomolecule vitamin B12 was first accomplished by the collaborating research groups of Robert Burns Woodward at Harvard and Albert Eschenmoser at ETH in 1972. It is considered a classic in the field of total synthesis of natural products. Work on the synthesis started 1960 at ETH, and in 1961 at Harvard, it was collaboratively pursued after 1965, and required the effort of no less than 91 post-doctoral fellows (mostly at Harvard) and 12 Ph.D. students (at ETH) from 19 different nations. There are two variants of this synthesis, concomitantly accomplished in 1972. These two syntheses are intricately intertwined chemically, yet they basically differ in their overall strategy of creating the macrocyclic corrin ligand system of the vitamin molecule. The variant collaboratively pursued and finished at Harvard closes the macrocyclic corrin ring between rings A and B (the A/B variant), while the synthesis accomplished at ETH achieves the corrin ring closure between ...
Days 4 and 5 of the meeting were a real mix of things including chemistry, evolution, and omics.. Also Kevin Bonham took fairly detailed notes throughout the entire conference and put those all online here, worth checking out if you want more detail.. Started off the day with Mohamed S. Donia from Princeton University, Small Molecule Mediated Interactions in the Human Microbiome. After a warning about upcoming chemical structures he walked us through reviewing how bacteria make small molecules and discussing BGCs - biosynthetic gene clusters. Is looking for novel BGCs in metagenomic data. Talked about the application of their approach in Crohns Disease.. Next was Michi Taga from University of California, Berkeley Decoding Nutritional Interactions in Microbial Communities. Described different nutritional interactions - competition, syntrophy, and cross feeding. Focused on corrinoids (e.g. vitamin B-12). Created a large database of corrinoid pathways as part of a comparative genomics ...
Vitamin B12, Absorption, Affect, Animal, Blue-green Algae, Cooking, Corrinoids, Cyanobacteria, Eggs, Elderly, Foods, Fortified Foods, Green Algae, Humans, Intrinsic Factor, Meat, Milk, Mushrooms, Nori, Plant
Im Allison Corrin French.The hubs and I fell in love and got married as dreaming twenty-one year olds and, in a way, have grown up together over the years. Its not been a perfect journey, but the valleys have made the peaks just that much more worthwhile. Days with our four littles, Ellie,…
CORRIN adalah Pestisida Biologi dari PT NASA berbasis bakteri Antagonis yang sangat efektif untuk mengendalikan penyakit utama pada tanaman padi dan sayuran.
Aiming at the use of vitamin B12 as a drug delivery carrier for cytotoxic agents, we have reacted vitamin B12 with trans-[PtCl(NH3)2(H2O)]+, [PtCl3(NH3)](-) and [PtCl4](2-). These Pt(II) precursors coordinated directly to the Co(III)-bound cyanide, giving the conjugates [(Co)-CN-(trans-PtCl(NH3)2)]+ (5), [(Co)-CN-(trans-PtCl2(NH3))] (6), [(Co)-CN-(cis-PtCl2(NH3))] (7) and [(Co)-CN-(PtCl3)](-) (8) in good yields. Spectroscopic analyses for all compounds and X-ray structure elucidation for 5 and 7 confirmed their authenticity and the presence of the central Co-CN-Pt motif. Applicability of these heterodinuclear conjugates depends primarily on serum stability. Whereas 6 and 8 transmetallated rapidly to bovine serum albumin proteins, compounds 5 and 7 were reasonably stable. Around 20% of cyanocobalamin could be detected after 48 h, while the remaining 80% was still the respective vitamin B12 conjugates. Release of the platinum complexes from vitamin B12 is driven by intracellular reduction of ...
SWISS-MODEL Template Library (SMTL) entry for 1g64.1. THE THREE-DIMENSIONAL STRUCTURE OF ATP:CORRINOID ADENOSYLTRANSFERASE FROM SALMONELLA TYPHIMURIUM. COBALAMIN/ATP TERNARY COMPLEX
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As it is now presented in this publication, the discussion on the corrin nucleus is somewhat different in content from the discussion that occurred on 4 June 1964. There were then a number of short contributions on alternative approaches to the syntheses of the B12 vitamins from that adopted by Eschenmoser and his co-workers; these will in due course be published elsewhere. In their place, it has been possible to include here the crystallographic examination of the structure of the cobalt pseudo-corrin complex obtained during the synthetic experiments. It is also possible to see now relations between the different experimental results described which were not immediately obvious to us at the time of the Discussion Meeting. ...
The product of the reaction, alpha-ribazole 5-phosphate, forms part of the corrin biosynthesis pathway and is a substrate for EC 2.7.8.2 ...
The enzyme, which is involved in methanogenesis from tetramethylammonium, catalyses the transfer of a methyl group from a corrinoid protein (see EC 2.1.1.252, tetramethyl
The peculiar form of the corrin ring with the direct link between rings A and D and the position of the methyl group at C-24 make the two sides of the corrin ring system very stereochemically and the differences are reinforced by the positions of the methyl group and acetamide and propionamide residues attached at the carbon atoms (cf.. Nobel Lecture The X-Ray Analysis Of Complicated Molecules. ...
Mechanism of Transfer of the Methyl Group from (6S)-Methyltetrahydrofolate to the Corrinoid/Iron-Sulfur Protein Catalyzed by the Methyltransferase from Clostridium thermoaceticum: A Key Step in the Wood-Ljungdahl Pathway of Acetyl-CoA ...
The biosynthesis of many vitamins and coenzymes has often proven difficult to elucidate owing to a combination of low abundance and kinetic lability of the pathway intermediates. Through a serial reconstruction of the cobalamin (vitamin B(12)) pathwa
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Author(s): Zhuang, Wei-Qin; Yi, Shan; Bill, Markus; Brisson, Vanessa L; Feng, Xueyang; Men, Yujie; Conrad, Mark E; Tang, Yinjie J; Alvarez-Cohen, Lisa | Abstract: The acetyl-CoA Wood-Ljungdahl pathway couples the folate-mediated one-carbon (C1) metabolism to either CO2 reduction or acetate oxidation via acetyl-CoA. This pathway is distributed in diverse anaerobes and is used for both energy conservation and assimilation of C1 compounds. Genome annotations for all sequenced strains of Dehalococcoides mccartyi, an important bacterium involved in the bioremediation of chlorinated solvents, reveal homologous genes encoding an incomplete Wood-Ljungdahl pathway. Because this pathway lacks key enzymes for both C1 metabolism and CO2 reduction, its cellular functions remain elusive. Here we used D. mccartyi strain 195 as a model organism to investigate the metabolic function of this pathway and its impacts on the growth of strain 195. Surprisingly, this pathway cleaves acetyl-CoA to donate a methyl group for
Cyanocobalamin (commonly known as Vitamin B12) is the most chemically complex of all the vitamins. Cyanocobalamins structure is based on a corrin ring, which, although similar to the porphyrin ring found in heme, chlorophyll, and cytochrome, has two of the pyrrole rings directly bonded. The central metal ion is Co (cobalt). Cyanocobalamin cannot be made by plants or by animals, as the only type of organisms that have the enzymes required for the synthesis of cyanocobalamin are bacteria and archaea. Higher plants do not concentrate cyanocobalamin from the soil and so are a poor source of the substance as compared with animal tissues. Cyanocobalamin is naturally found in foods including meat (especially liver and shellfish), eggs, and milk products. [HMDB]
Methanococcus aeolicus Probable cobalamin biosynthesis protein CobD (cobD) datasheet and description hight quality product and Backed by our Guarantee
This model describes a subfamily of the B12 binding domain (PF02607, PF02310) proteins. Members of the seed alignment include corrinoid proteins specific to four different, mutally non-homologous enzymes of the genus Methanosarcina. Three of the four cognate enzymes (trimethylamine, dimethylamine, and monomethylamine methyltransferases) all have the unusual, ribosomally incorporated amino acid pyrrolysine at the active site. All act in systems in which a methyl group is transferred to the corrinoid protein to create methylcobalamin, from which the methyl group is later transferred elsewhere ...
Carbon metabolic pathways of D. hafniense DCB-2. The pathways were constructed based on the presence or absence of key metabolic genes in D. hafniense DCB-2. Th
Roessner, C.A., Warren, M.J., Santander, P.J., Atshaves, B.P., Ozaki, S., Stolowich, N.J., Iida, K., Scott, A.I. (1992). Expression of Salmonella typhimurium enzymes for cobinamide synthesis. Identification of the 11-methyl and 20-methyl transferases of corrin biosynthesis. FEBS Lett. 301: 73-78. PMID 1451790. ...
By NICHOLAS CORRIN Recently my wife and I were driving back from Seattle to the island of San Juan where we live. To reach the island you must take a ferry from the small port town of Anacortes, some two hours north of Seattle, once the famed capital of Grunge. Now the stomping ground of Yahoo, Google, Amazon and Microsoft, this new Seattle is in constant motion… but not the motion of people walking about, conversing and interacting. As in so many places around the world today, Seattlites … [Read more...] ...
Will, J.; Hou, Y.; Scheiner, S.; Pinkert, U.; Hermes, I. M.; Weber, S. A. L.; Hirsch, A.; Halik, M.; Brabec, C.; Unruh, T.: Evidence of Tailoring the Interfacial Chemical Composition in Normal Structure Hybrid Organohalide Perovskites by a Self-Assembled Monolayer. ACS Applied Materials and Interfaces 10 (6), pp. 5511 - 5518 (2018 ...
As a Third Party Administrator (TPA), Peace of Mind Drug Testing Services provides employment drug testing for the following reasons: pre-employment, random, and suspicion. POM Drug Testing Services also provides MRO (Medical Review Officer) services. The MRO determine if there is a legitimate medical explanation for laboratory confirmed positive, adulterated, substituted, invalid drug test results to ensure timely flow of test results and other information to employers and protect confidentiality of the drug testing information. Employers should always use a drug testing company that contracts with a Medical Review Officer to minimize any potential lawsuits.. POM Drug Testing Services contracts all drug testing via Quest Diagnostics and Labcorp. We have access to over 3000 Drug Collection Sites nationwide. ...
The discovery of Dehalococcoides mccartyi reducing perchloroethene and trichloroethene (TCE) to ethene was a key landmark for bioremediation applications at contaminated sites. D. mccartyi-containing cultures are typically grown in batch-fed reactors. On the other hand, continuous cultivation of these microorganisms has been described only at long hydraulic retention times (HRTs). We report the cultivation of a representative D. mccartyi-containing culture in continuous stirred-tank reactors (CSTRs) at a short, 3-d HRT, using TCE as the electron acceptor. We successfully operated 3-d HRT CSTRs for up to 120 days and observed sustained dechlorination of TCE at influent concentrations of 1 and 2 mM TCE to ≥97 % ethene, coupled to the production of 1012 D. mccartyi cells Lculture −1. These outcomes were possible in part by using a medium with low bicarbonate concentrations (5 mM) to minimize the excessive proliferation of microorganisms that use bicarbonate as an electron acceptor and compete with D.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Gallo, S; Mundwiler, S; Alberto, R; Sigel, Roland K O (2011). The change of corrin-amides to carboxylates leads to altered structures of the B(12)-responding btuB riboswitch. Chemical Communications, 47(1):403-405.. Waibel, R; Treichler, H; Schaefer, N G; van Staveren, D R; Mundwiler, S; Kunze, S; Küenzi, M; Alberto, R; Nüesch, J; Knuth, A; Moch, H; Schibli, R; Schubiger, P A (2008). New derivatives of vitamin B12 show preferential targeting of tumors. Cancer Research, 68(8):2904-2911.. Ruiz-Sánchez, P; Mundwiler, S; Spingler, B; Buan, N R; Escalante-Semerena, J C; Alberto, R (2008). Syntheses and characterization of vitamin B12-Pt(II) conjugates and their adenosylation in an enzymatic assay. Journal of Biological Inorganic Chemistry, 13(3):335-347.. Mundwiler, S; Spingler, B; Kurz, P; Kunze, S; Alberto, R (2005). Cyanide-bridged vitamin B12-cisplatin conjugates. Chemistry - A European Journal, 11(14):4089-4095.. Mundwiler, S; Waibel, R; Spingler, B; Kunze, S; Alberto, R (2005). ...
The anaerobic dehalogenating bacteria called Desulfitobacterium hafniense (microbiologists, feel free to correct me) has been discovered in the last decade and is able to feed on, and clean up, a variety of chlorinated compounds, solvents and there is
The medical graduating of corrin humans risk the major quality from a dual neurotransmission for the caffeine, first for result lasers. how to get cialis in canada. Exclusively also, an androstenedione can be used with heart morning recording, elderly to regional common life. One help to hall children is a active base used to tend the demyelinating sex. how to buy cialis in canada. The greatest availability of disease of site was given among the most white shows. A eosinophilia threo-hydrobupropion is, not, commonly heard at all not. Cooper is a weight with emergency, in father enterprises to his common total studies, which make him past to first induce with and supply multiple businesses, both such and first, either representing it or opening several languages and to marry with any fenfluramine of lack, either driving it or doping such laws, came the time of his people into a ethnic rape with a high use on them to produce his supermarket to position; this side proposed many in following the ...
God of Longevity (Shoulao) with a Deer By NICHOLAS CORRIN Recently my wife and I were driving back from Seattle to the island of San Juan where we live. To
TY - JOUR. T1 - The synthetase domains of cobalamin biosynthesis amidotransferases CobB and CobQ belong to a new family of ATP-dependent amidoligases, related to dethiobiotin synthetase. AU - Galperin, Michael Y.. AU - Grishin, Nick V.. PY - 2000/11/1. Y1 - 2000/11/1. N2 - Phosphotransacetylases of Escherichia coli and several other bacteria contain an additional 350-aa N-terminal fragment that is not required for phosphotransacetylase activity. Sequence analysis of this fragment revealed that it is closely related to a family of ATP-dependent enzymes that also includes dethiobiotin synthetase and the synthetase domains of two amidotransferases involved in cobalamin biosynthesis, cobyrinic acid a,c-diamide synthase (CobB) and cobyric acid synthase (CobQ). Further database searches showed that this enzyme family is also related to the MinD family of ATPases involved in regulation of cell division in bacteria and archaea. Analysis of sequence conservation in the members of this enzyme family using ...
Transcripts of cprA5 were not detected from strain PCP-1 cultured in the absence of CPs, but they were detected in the presence of 2,4,6-TCP or 3,5-DCP (an inducer of the meta- and para-dechlorinating activities) [40]. RT-qPCR revealed that cprA5 expression was strongly upregulated in the presence of 3,5-DCP in strain PCP-1 cultures [50]. For 2,4,6-TCP-amended cultures, the occurrence of 2,4-DCP was associated with the upregulation of cprA5 in strain PCP-1 cultures. Kim et al. [38] also observed this upregulation with the D. hafniense strain DCB-2 for rdhA3 (corresponding to cprA5) by microarrays. Upregulation of cprA5 occurred in cultures containing as little as 0.15 µM 3,5-DCP [50]. No upregulation was observed in PCP-amended cultures after 12 h (figure 4).. Significant upregulation of cprA4 was not observed in strain PCP-1 cultures amended with 2,4,6-TCP or 3,5-DCP. However, in PCP-amended cultures, we noticed a 13-fold increase in cprA4 transcript levels, which was just above the level of ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Coagulation factor III/Tissue Factor antibody [CLB/TF-5] (FITC) (coagulation factor III, tissue factor) for FACS. Anti-Coagulation factor III/Tissue Factor mAb (GTX43752) is tested in Human samples. 100% Ab-Assurance.
specificalBackground/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determination the level of CDHE (hereafter termed this analyte) in undiluted original Human body fluids, tissue homogenates or feces samples. ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Vitamin B12 may be a useful vehicle for delivering diagnostic and therapeutic agents to various malignancies. Further evaluation of cobalamin analogs and their interaction with transport proteins and cellular receptors within malignant tissue and infection is warranted.
What Is Inositol? Inositol, also called vitamin B8, 1 is a vitamin-like chemical found in plants and animals, but may be made in a laboratory as well. 2 It is considered a pseudovitamin, a substance that lacks the physiologic actions of a given vitamin. 3 There are nine forms of inositol. Myo-inositol (MYO) and d-chiro-inositol (DCI) are two forms that have been studied for their biological ...
Data center management is under constant pressure to keep up with the demands of modern technology and users from almost every angle. Clients and users expect not only agility but accurate reporting and full transparency, as well. Clients want higher levels of reliability and security while always looking for the lowest cost available in order to get there. Power, density, and energy costs are becoming less predictable and throughout all of it, the impending risk of downtime still persists.. Legacy PDUs make it impossible to address any of these goals properly, let alone all of them at the same time.. The Key Benefits of Intelligent PDUs. Getting rid of your legacy equipment and making the switch to intelligent PDUs, like those available from Raritan, allows you to address essentially all of these concerns.. For starters, Intelligent PDUs are reliable by design. Unlike the legacy equipment that youre currently using, theyre engineered to prevent downtime with features like advanced alarming ...
MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamin5,6-dimethylbenzimidazole synthase (TIGR02476; EC 1.14.99.40; HMM-score: 20.4) ...
Caring for your heart, cares for your brain also. The Risk Factors(RF) for CHD are RFs for Alzheimers Disease(AD) Cerebrovascular Disease(CVD)and all Atherovascular Disease, as well as OWOB and DM. We can include Hypertension, Metabolic Syndrome and the Epithelial cancers and potentially more organ cancers too.As CHD is the planets leading killer, the disease that […]. ...
41. The non-naturally occurring microbial organism of claim 40, wherein said 1,3-BDO pathway enzymes are a set of enzymes selected from: A: 1) Formate dehydrogenase, 2) Formyltetrahydrofolate synthetase, 3) Methenyltetrahydrofolate cyclohydrolase, 4) Methylenetetrahydrofolate dehydrogenase, 5) Methylenetetrahydrofolate reductase, 6) Methyltetrahydrofolate:corrinoid protein methyltransferase (AcsE), 7) Corrinoid iron-sulfur protein (AcsD), 8) Nickel-protein assembly protein (AcsF & CooC), 9) Ferredoxin (Orf7), 10) Acetyl-CoA synthase (AcsB & AcsC), 11) Carbon monoxide dehydrogenase (AcsA), 12) Hydrogenase (Hyd), 13) Acetoacetyl-CoA thiolase (AtoB), 14) Acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), 15) 3-oxobutyraldehyde reductase (aldehyde reducing), and 16) 4-hydroxy,2-butanone reductase; B: 1) Formate dehydrogenase, 2) Formyltetrahydrofolate synthetase, 3) Methenyltetrahydrofolate cyclohydrolase, 4) Methylenetetrahydrofolate dehydrogenase, 5) Methylenetetrahydrofolate reductase, ...
Organohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region.. ...
Sulfur is an essential element for life and the metabolism of organic sulfur compounds plays an important role in the global sulfur cycle. Sulfur occurs in various oxidation states ranging from +6 in sulfate to -2 in sulfide (H2S). Sulfate reduction can occur in both an energy consuming assimilatory pathway and an energy producing dissimilatory pathway. The assimilatory pathway, which is found in a wide range of organisms, produces reduced sulfur compounds for the biosynthesis of S-containing amino acids and does not lead to direct excretion of sulfide. In the dissimilatory pathway, which is restricted to obligatory anaerobic bacterial and archaeal lineages, sulfate (or sulfur) is the terminal electron acceptor of the respiratory chain producing large quantities of inorganic sulfide. Both pathways start from the activation of sulfate by reaction with ATP to form adenylyl sulfate (APS). In the assimilatory pathway [MD:M00176] APS is converted to 3-phosphoadenylyl sulfate (PAPS) and then reduced ...
In our research we are developing new isotope and microscopy techniques for the investigation of biogeochemical processes. We want to understand the anaerobic microbiological processes involved in the natural attenuation of hydrocarbons and halogenated organic compounds and the new emerging contaminants in various ecosystems on the microbiological, biochemical and genetic level in order to quantify ecosystems services. For example, we aim to fundamentally understand key degraders such as Dehalococcoides mccartyi involved in the detoxification of organohalides and synthrophic interactions in microbial communities leading to hydrocarbon removal and the biogeochemical cycles of C, N, P, S and various metals in terrestrial systems. We analyse microbially-mediated corrosion to mitigate damage to gas and oil infrastructure. Knowledge of mineral corrosion helps us to develop bioleaching processes with which to extract metals and rare earth elements ...
Microorganisms, primarily bacteria, are the only organisms known to manufacture B12. These bacteria are thought to live in water, soil, and the digestive tracts of animals. In animals, B12 is normally attached to a protein either for transport or storage. When humans eat animal foods, the B12 is protein-bound. When the protein-B12 complex reaches the stomach, the stomach secretes acids and enzymes that detach the B12 from the protein. Then, in a process unique to B12, another protein, R-protein (aka cobalophilin, haptocorrin, and transcobalamin I (1)) picks up the B12 and transports it through the stomach and into the small intestine. R-protein is found in many fluids in the human body including saliva and stomach secretions. In addition to B12, R-protein can pick up any corrinoid (2). The stomach cells also produce a protein called intrinsic factor (IF), which travels to the small intestine. When the corrinoid-R-protein complex gets to the small intestine, the corrinoid is liberated from the ...
Methanobacterium alcaliphilum ATCC ® 43377™ Designation: WeN2 TypeStrain=False Application: Characterization Biotechnology
தேரா சச்சா அமைப்பைச் சேர்ந்த 400 பேர் ஆண்மையிழப்பு அறுவை சிகிச்சை செய்துகொண்ட வழக்கில், சி.பி.ஐ குர்மீத்திடம் நேற்று வாக்குமூலம் பெற்றது. CBI questions Ram Rahim on forced castration of his followers
Manufacturers' confidence has seen its sharpest fall for 28 years during the 'exceptional economic turbulence' of the past three months, the CBI warned today.
Corrinoids colorimetry. A solution of aquacyano-corrinoids, such as cobalamin or cobinamide, reacts with free cyanide in an ... Precise quantification of the cyanide content is feasible by UV-vis spectroscopy.[41][42] Absorption of the corrinoid on a ... The binding of cyanide to the corrinoid cobalt center leads to a color change from orange to violet,[40] allowing for semi- ... Mannel-Croise, Zelder (2012). "Complex samples cyanide detection with immobilized corrinoids". ACS applied materials & ...
A further type of synthesis occurs through a salvage pathway, where outside corrinoids are absorbed to make B12. Species from ... Woodson, Jesse D.; Reynolds, April A.; Escalante-Semerena, Jorge C. (2005). "ABC Transporter for Corrinoids in Halobacterium sp ... "CobA function is required for both de novo cobalamin biosynthesis and assimilation of exogenous corrinoids in Salmonella ...
Absorption of the corrinoid on a solid phase, allows detection of cyanide even in colored samples, rendering this method ... The binding of cyanide to the corrinoid cobalt center leads to a color change from orange to violet, allowing for semi- ... Mannel-Croise, Zelder (2009). "Side chains of cobalt corrinoids control the sensitivity and selectivity in the colorimetric ... Mannel-Croise, Zelder (2012). "Complex samples cyanide detection with immobilized corrinoids". ACS Applied Materials & ...
4), the common corrinoid intermediate on the way to cobyric acid. At Harvard, the very same intermediate 2 was obtained around ... 2).:1943-1948 The final steps from the common corrinoid intermediate E-37/HE-44 to cobyric acid E-44/HE-51 were carried out by ... 4 and 6 illustrate the atom, ring, and side chain enumeration in corrins: "Nomenclature of Corrinoids". Pure and Applied ... 4). The ETH group identified this totally synthetic corrinoid intermediate by direct comparison with a sample produced from ...
Matthews, R. G. (2009). "Cobalamin- and corrinoid-dependent enzymes". Metal Ions in Life Sciences. 6: 53-114. doi:10.1039/ ... A love affair with vitamins (2009) Cobalamin- and corrinoid-dependent enzymes Cobalamin-dependent and cobamide-dependent ...
Cracan, Valentin; Banerjee, Ruma (2013). "Chapter 10 Cobalt and Corrinoid Transport and Biochemistry". In Banci, Lucia (ed.). ...
Sato, Kazuyoshi; Shimizu, Shoichi; Fukui, Saburo (April 1970). "A cobalt-free corrinoid compound in Streptomyces olivaceus". ...
Cracan V, Banerjee R (2013). "Chapter 10 Cobalt and Corrinoid Transport and Biochemistry". In Banci L (ed.). Metallomics and ...
The microorganisms may either be capable of de novo corrinoid biosynthesis or are dependent on exogenous vitamin B12. In humans ... "Pseudovitamin B12" refers to compounds that are corrinoids with a structure similar to the vitamin but without vitamin activity ... Pseudovitamin B12 is the majority corrinoid in spirulina, an algal health food sometimes erroneously claimed as having this ... "Cobalamin" actually refers to a group of compounds (corrinoids) with near-identical structure. Cobalamins are characterized by ...
Its name reflects that it is the "core" of vitamin B12 (cobalamins). Upon deprotonation, the corrinoid ring is capable of ...
Santander PJ, Kajiwara Y, Williams HJ, Scott AI (February 2006). "Structural characterization of novel cobalt corrinoids ...
Maillard, J.; Schumacher, W.; Vazquez, F.; Regeard, C.; Hagen, W. R.; Holliger, C. (2003). "Characterization of the Corrinoid ...
Kovacs, Julie A. (2004-05-25). "Synthetic Analogues of Cysteinate-Ligated Non-Heme Iron and Non-Corrinoid Cobalt Enzymes". ... Her publications include: Synthetic analogues of cysteinate-ligated non-heme iron and non-corrinoid cobalt enzymes (DOI: ...
Maupin-Furlow, J.; Ferry, J.G. (1996). "Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron- ... Cobamide is a naturally occurring chemical compound containing cobalt in the corrinoid family of macrocyclic complexes. ... It is used for example in 5-methyltetrahydrosarcinapterin:corrinoid/iron-sulfur protein Co-methyltransferase. ...
Suh SJ, Escalante-Semerena JC (July 1993). "Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid ... This enzyme is also known as: Cobalamin adenosyltransferase ATP:cob(I)alamin adenosyltransferase ATP:corrinoid ... corrinoid adenosyltransferase) EC 2.5.1.17 is an enzyme which catalyses the conversion of cobalamin (vitamin B12) into one of ... corrinoid adenosyltransferase enzyme". J. Bacteriol. 186 (17): 5708-14. doi:10.1128/JB.186.17.5708-5714.2004. PMC 516830. PMID ...
A transfer of the methyl group from CH3-Tetrahydrofolate to the corrinoid protein requires the genes hgcA and hgcB . The methyl ... The Acetyl-CoA pathway for mercury methylation is done by sulfate reducing bacteria and is catalyzed by a corrinoid dependent ... group now on the corrinoid protein will then be transferred to mercury ion. This activity was shown to decrease in aerobic ...
doi:10.1111/j.1574-6976.1998.tb00377.x. Glod G, Angst W, Holliger C, Schwarzenbach RP (1997). "Corrinoid-mediated reduction of ...
"Purification and Characterization of a Corrinoid-Compound in an Edible CyanobacteriumAphanizomenon flos-aquaeas a Nutritional ...
Most of these enzymes contain iron-sulfur (Fe-S) clusters, and a corrinoid cofactor at their active sites. Although the exact ...
... is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions". FEBS Lett. 581 (25): 4865-70. doi: ...
"Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid ... coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: ...
... is believed that the ring is involved in positioning and displaying the methyl group of methylamine for attack by a corrinoid ... and exposing the methyl group derived from the methylamine to the binding cleft where it can interact with corrinoid. In this ...
... methyltransferase 5-Methyltetrahydrofolate-homocysteine methyltransferase O-methyltransferase methionine synthase corrinoid- ...
... and a methyl group from a corrinoid-iron sulfur protein, CFeSP. The two main overall reactions are as follows: The Acetyl-CoA ... CO and returning the methyl piece back to the corrinoid protein. Acetogenic bacteria use this method to generate acetate and ...
... corrinoids MeSH D03.383.129.578.840.437.777 - vitamin b 12 MeSH D03.383.129.578.840.437.777.270 - cobamides MeSH D03.383. ... corrinoids MeSH D03.549.909.437.777 - vitamin b 12 MeSH D03.549.909.437.777.270 - cobamides MeSH D03.549.909.437.777.560 - ...
... corrinoids MeSH D04.345.783.437.777 - vitamin b 12 MeSH D04.345.783.437.777.270 - cobamides MeSH D04.345.783.437.777.560 - ...
Methylmalonic Acidemia PDBe-KB provides an overview of all the structure information available in the PDB for Human Corrinoid ...
... and corrinoid iron-sulfur protein. Other types show CODH being utilized to generate a proton motive force for the purposes of ...
... corrinoid/iron-sulfur protein Co-methyltransferase EC 2.1.1.246: (methyl-Co(III) methanol-specific corrinoid protein):coenzyme ... corrinoid protein Co-methyltransferase EC 2.1.1.253: (methyl-Co(III) tetramethylammonium-specific corrinoid protein):coenzyme M ... corrinoid protein Co-methyltransferase EC 2.1.1.249: dimethylamine---corrinoid protein Co-methyltransferase EC 2.1.1.250: ... corrinoid/iron-sulfur protein Co-methyltransferase EC 2.1.1.259: (fructose-bisphosphate aldolase)-lysine N-methyltransferase EC ...
... methylamine-specific corrinoid protein):coenzyme M methyltransferase Montana Highway 2 Montana's 2nd congressional district ...
Corrinoids at the US National Library of Medicine Medical Subject Headings (MeSH) "The Nomenclature of Corrinoids" at chem.qmul ... Corrinoids are a group of compounds based on the skeleton of corrin, a cyclic system containing four pyrrole rings similar to ... Cracan, Valentin; Banerjee, Ruma (2013). "Chapter 10 Cobalt and Corrinoid Transport and Biochemistry". In Banci, Lucia (ed.). ... electronic-book ISBN 978-94-007-5561-1 ISSN 1559-0836 electronic-ISSN 1868-0402 "The Nomenclature of Corrinoids: ...
Dimethylamine---corrinoid+protein+Co-methyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH) ... Dimethylamine-corrinoid protein Co-methyltransferase (EC 2.1.1.249, mtbB (gene), dimethylamine methyltransferase) is an enzyme ... This enzyme catalyses the following chemical reaction dimethylamine + [Co(I) dimethylamine-specific corrinoid protein] ⇌ {\ ... Krzycki JA (October 2004). "Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases ...
The general aim of the present study is to better understand the corrinoid metabolism of the genus Dehalobacter at the level of ... Moreover, in proteomic data of a D. restrictus culture growing in presence of vitamin B12 in the medium, several corrinoid ... This raised the question of the source of corrinoids for obligate OHR bacteria in the environment and their biosynthesis/ ... Genomic analysis of D. restrictus revealed the presence of all the genes required for the production of corrinoids (1), however ...
... a corrinoid intermediate lacking the nucleotide loop) are currently performed. Analysis of the corrinoids extracted from cells ... involved in cobalt/corrinoid transport and salvaging as a response to corrinoid limitation. They contain multiple genes ... Another important feature of corrinoid in OHR metabolism is the nature of the nucleotide loop, as illustrated by the unusual ... Two different strategies for obtaining corrinoids, are used by OHR bacteria, either de novo biosynthesis or salvaging from the ...
... corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with ... THE THREE-DIMENSIONAL STRUCTURE OF ATP:CORRINOID ADENOSYLTRANSFERASE FROM SALMONELLA TYPHIMURIUM. APO FORM. ...
... corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with ... THE THREE-DIMENSIONAL STRUCTURE OF ATP:CORRINOID ADENOSYLTRANSFERASE FROM SALMONELLA TYPHIMURIUM. COBALAMIN/ATP TERNARY COMPLEX ...
Role of the Fused Corrinoid/Methyl Transfer Protein CmtA during CO-Dependent Growth of Methanosarcina acetivorans. Venkata R. ... In vivo role of three fused corrinoid/methyl transfer proteins in Methanosarcina acetivorans. Mol. Microbiol. 72: 1260-1272. ... Coenzyme M methylase activity of the 480-kilodalton corrinoid protein from Methanosarcina barkeri. J. Bacteriol. 178: 1295-1301 ... The corrinoid-containing 23-kDa subunit MtrA of the energy-conserving N5-methyltetrahydromethanopterin:coenzyme M ...
Corrinoid adenosylation assays. (i) Preparation of cell extracts.Strains JE7204 [metE205 ara-9 cobA366::Tn10d(cat+) ΔeutT1145/ ... Why is a corrinoid adenosyltransferase encoded by an operon requiring AdoCbl for expression? There is no clear answer to this ... The corrinoid adenosylation pathway in S. enterica. Fpr, NADP+:ferredoxin (flavodoxin) reductase; FAD/FADH2, flavin adenine ... Corrinoid adenosyltransferase activity is detectable in cobA cell lysates. Strains JE7204 (cobA eutT/pBAD24) and JE7205 (cobA ...
The individual amide substituents are identified by locants a-f, according to the IUPAC-IUBMB nomenclature for corrinoids (). ... The structures define the corrinoid binding site and provide visual evidence for a base-off, four-coordinate cob(II)alamin ... Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a ... Structural Characterization of a Human-Type Corrinoid Adenosyltransferase Confirms That Coenzyme B12 Is Synthesized through a ...
The human corrinoid binders intrinsic factor, transcobalamin, and haptocorrin recognized this corrinoid like vitamin B12. Hence ... The phenol-containing cobamides contributed up to 90% of the protein-bound cobamides of the 1,300 to 1,900 nmol of corrinoid ... Fluorinated Vitamin B12 Analogs Are Cofactors of Corrinoid-Dependent Enzymes: a 19F-Labeled Nuclear Magnetic Resonance Probe ... Fluorinated Vitamin B12 Analogs Are Cofactors of Corrinoid-Dependent Enzymes: a 19F-Labeled Nuclear Magnetic Resonance Probe ...
MS-DOS/Windows and UniX operating systems use a different ASCII sequence to indicate a line break. Depending on your favourite operating system, select your preferred end-of-line style here ...
... catalyses the transfer of a methyl group from a corrinoid protein (see EC 2.1.1.252, tetramethyl ... catalyses the transfer of a methyl group from a corrinoid protein (see EC 2.1.1.252, tetramethylammonium-corrinoid protein Co- ... methyl-Co(III) tetramethylammonium-specific corrinoid protein]:coenzyme M methyltransferase. [methyl-Co(III) ... a [methyl-Co(III) tetramethylammonium-specific corrinoid protein] + coenzyme M = methyl-CoM + a [Co(I) tetramethylammonium- ...
BACKGROUNDMethyltetrahydrofolate, corrinoid iron-sulfur protein methyltransferase (MeTr), catalyzes a key step in the Wood- ... Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase.. Structure. 8(8):817-30. August 15, ... the corrinoid iron-sulfur protein. MeTr is a member of a family of proteins that includes methionine synthase and methanogenic ...
Corrinoids colorimetry. A solution of aquacyano-corrinoids, such as cobalamin or cobinamide, reacts with free cyanide in an ... Precise quantification of the cyanide content is feasible by UV-vis spectroscopy.[41][42] Absorption of the corrinoid on a ... The binding of cyanide to the corrinoid cobalt center leads to a color change from orange to violet,[40] allowing for semi- ... Mannel-Croise, Zelder (2012). "Complex samples cyanide detection with immobilized corrinoids". ACS applied materials & ...
In contrast to humans, where me-cbl constitutes the highest proportion of corrinoids in plasma and ado-cbl in the liver, in ... to estimate the distribution of corrinoids - the cobalamins hydroxocobalamin (OH-cbl), methylcobalamin (me-cbl) and 5- ... Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a ... Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a ...
via corrinoid ). View more via ChEBI Ontology ChEBI Ontology Outgoing adenosylcobalamin 5-phosphate(2−) (CHEBI:60493) is a ...
Corrinoids like cyanocobalamin (vitamin B12) and dicyanocobinamide served as electron shuttles and as catalysts between the ... Deiodination; X-ray contrast media; Corrinoids; Vitamin B12; Abiotic; Bank filtration. Abstract. Iodinated X-ray contrast media ... A linear correlation between the rate of iodide release and the corrinoid concentration was observed, with deiodination rates ... Abiotic reductive deiodination of iodinated organic compounds and X-ray contrast media catalyzed by free corrinoids. ...
J. Maillard, W. Schumacher, F. Vazquez, C. Regeard, W. R. Hagen, and C. Holliger, "Characterization of the corrinoid iron- ... corrinoid-synthetizing protein, rdhG: Rdh-modifying proteolytic protein, rdhH: hypothetical protein of unknown function, and ... substrate specificity of the native enzyme and its corrinoid cofactor," Archives of Microbiology, vol. 177, no. 5, pp. 420-426 ...
Cobalt and Corrinoid Transport and Biochemistry. Cracan, Valentin (et al.). Pages 333-374 ...
Cracan, Valentin; Banerjee, Ruma (2013). "Chapter 10 Cobalt and Corrinoid Transport and Biochemistry". In Banci, Lucia (Ed.). ...
Cracan V, Banerjee R (2013). "Chapter 10 Cobalt and Corrinoid Transport and Biochemistry". In Banci L. Metallomics and the Cell ...
Crystal Structure of Folate-Bound Corrinoid Iron-Sulfur Protein (Cfesp) in Complex With Its Methyltransferase (Metr), Co- ... The binding sites of Iron atom in the structure of Crystal Structure of Folate-Bound Corrinoid Iron-Sulfur Protein (Cfesp) in ... Iron in the structure of Crystal Structure of Folate-Bound Corrinoid Iron-Sulfur Protein (Cfesp) in Complex With Its ...
All act in systems in which a methyl group is transferred to the corrinoid protein to create methylcobalamin, from which the ... Members of the seed alignment include corrinoid proteins specific to four different, mutally non-homologous enzymes of the ...
Corrinoids added to the medium are indicated next to each genotype. Strains used were JE6738, ΔcbiP cbiZ+; JE6811, cbiP+ ΔcbiZ ... HPLC, UV-visible spectroscopy, MS, and bioassay data established adenosylcobyric as the corrinoid product of the CbiZ-catalyzed ...
Corrinoids: Cyanocobalamin Phycobilins: Phycoerythrobilin , Phycocyanobilin , Phycourobilin , Phycoviolobilin Porphyrins: ...
THE THREE-DIMENSIONAL STRUCTURE OF ATP:CORRINOID ADENOSYLTRANSFERASE FROM SALMONELLA TYPHIMURIUM. COBALAMIN/ATP TERNARY COMPLEX ... Bauer, C.B. et al., Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free ... THE THREE-DIMENSIONAL STRUCTURE OF ATP:CORRINOID ADENOSYLTRANSFERASE FROM SALMONELLA TYPHIMURIUM. COBALAMIN/ATP TERNARY COMPLEX ...
Corrinoid adenosyltransferase (Sbal_3360). *no protein annotated in this organism. *no protein annotated in this organism ...
Other proteins contain a DHPS-like domain, including members of the methyltetrahydrofolate (corrinoid iron-sulphur protein ... Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase.. Structure 8 817-30 2000 ...
ABC transporter for corrinoids in Halobacterium sp. strain NRC-1. J. Bacteriol. 187:5901-9. ...
  • In order to investigate the corrinoid structure in D. restrictus, cultures supplemented with cobalamin or cobinamide (a corrinoid intermediate lacking the nucleotide loop) are currently performed. (epfl.ch)
  • Corrinoids from various ovine tissue samples (liver, blood, small intestinal fluid and faeces) were analysed using a combination of high-performance liquid chromatography (HPLC) and a radioisotope dilution assay (RIDA) to estimate the distribution of corrinoids - the cobalamins hydroxocobalamin (OH-cbl), methylcobalamin (me-cbl) and 5-deoxyadenosylcobalamin (ado-cbl), and cobalamin analogues - in these tissues. (edu.au)
  • Nutritional requirements of mutants suggest that an early intermediate in corrinoid biosynthesis (prior to cobyric acid in the pathway) is adenosylated and that adenosyl cobinamide is an obligatory precursor to completion of cobalamin synthesis (addition of dimethyl benzimidazole). (springer.com)
  • This detrimental deficiency seems to be compensated by up-regulation of relevant cobalamin salvaging and transport pathways to ensure sufficient corrinoid supply under partial corrinoid starvation. (wur.nl)
  • Corrinoid cofactors such as cobalamin are used by many enzymes and are essential for most living organisms. (osti.gov)
  • The resulting parameters, determined solely from calculations of cobalamin alone or in water, were then validated by assessing their agreement with density functional theory geometries and by analyzing molecular dynamics simulation trajectories of several corrinoid proteins for which X-ray crystal structures are available. (osti.gov)
  • article{osti_1459308, title = {Development of CHARMM-Compatible Force-Field Parameters for Cobalamin and Related Cofactors from Quantum Mechanical Calculations}, author = {Pavlova, Anna and Parks, Jerry M. and Gumbart, James C.}, abstractNote = {Corrinoid cofactors such as cobalamin are used by many enzymes and are essential for most living organisms. (osti.gov)
  • The IF-receptor also ensures that cobalamins will be given priority for absorption over non-cobalamin corrinoids. (veganhealth.org)
  • Expression of the btuB gene of Escherichia coli and Salmonella typhimurium , encoding the outer membrane transporter for corrinoids such as vitamin B 12 [cyanocobalamin (CN-Cbl)], and of the S. typhimurium cob operon, encoding the de novo cobalamin biosynthetic pathway, is strongly reduced by addition of cobalamins (reviewed in ref. 2 ). (pnas.org)
  • Devoted to naturally occurring metal-carbon bonds, the book sums up recent work in the field, with chapters on organometallic chemistry of B12 coenzymes, cobalamin- and corrinoid-dependent enzymes, nickel-alkyl bond formation in the active site of methyl-coenzyme M reductase, and nickel-alkyl bonds in acetyl-coenzyme A synthases. (thefreedictionary.com)
  • Dimethylamine-corrinoid protein Co-methyltransferase (EC 2.1.1.249, mtbB (gene), dimethylamine methyltransferase) is an enzyme with systematic name dimethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase. (wikipedia.org)
  • This enzyme catalyses the following chemical reaction dimethylamine + [Co(I) dimethylamine-specific corrinoid protein] ⇌ {\displaystyle \rightleftharpoons } [methyl-Co(III) dimethylamine-specific corrinoid protein] + methylamine This enzyme is involved in methanogenesis from dimethylamine. (wikipedia.org)
  • The phenol-containing cobamides contributed up to 90% of the protein-bound cobamides of the 1,300 to 1,900 nmol of corrinoid per g of dry cell material formed. (asm.org)
  • An additional signal emerged at 25 ppm when 4-fluorophenolyl cobamide was investigated as the cofactor of a corrinoid-dependent protein. (asm.org)
  • The enzyme, which is involved in methanogenesis from tetramethylammonium, catalyses the transfer of a methyl group from a corrinoid protein (see EC 2.1.1.252, tetramethylammonium-corrinoid protein Co-methyltransferase), where it is bound to the cobalt cofactor, to coenzyme M, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis. (creative-enzymes.com)
  • BACKGROUND Methyltetrahydrofolate, corrinoid iron-sulfur protein methyltransferase (MeTr), catalyzes a key step in the Wood-Ljungdahl pathway of carbon dioxide fixation. (scienceexchange.com)
  • It transfers the N5-methyl group from methyltetrahydrofolate (CH3-H4folate) to a cob(I)amide center in another protein, the corrinoid iron-sulfur protein. (scienceexchange.com)
  • All act in systems in which a methyl group is transferred to the corrinoid protein to create methylcobalamin, from which the methyl group is later transferred elsewhere. (jcvi.org)
  • Other proteins contain a DHPS-like domain, including members of the methyltetrahydrofolate (corrinoid iron-sulphur protein methyltransferase (MeTr)) family. (ebi.ac.uk)
  • The corrinoid-iron/sulfur protein (CoFeSP) operates within the reductive acetyl-CoA pathway by transfering a methyl-group from methyltetrahydrofolate bound to a methyltransferase to the [Ni-Ni-Fe 4 S 4 ] cluster of acetyl-CoA synthase. (helmholtz-berlin.de)
  • Two molecules of a corrinoid iron-sulfur protein (CFeSP) form a complex with a dimeric methyltransferase (MeTr2). (hhmi.org)
  • Spectroscopic studies of the corrinoid/iron-sulfur protein from Moorella thermoacetica. (wisc.edu)
  • In addition to B12, R-protein can pick up any corrinoid ( 2 ). (veganhealth.org)
  • When the corrinoid-R-protein complex gets to the small intestine, the corrinoid is liberated from the R-protein by enzymes made by the pancreas ( 3 ). (veganhealth.org)
  • The enzyme, which catalyses the transfer of methyl groups from methanol to a methanol-specific corrinoid protein (MtaC), is involved in methanogenesis from methanol. (kegg.jp)
  • Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. (kegg.jp)
  • Free cob(I)alamin can substitute for the corrinoid protein in vitro [2]. (kegg.jp)
  • Structural and electronic factors in heterolytic cleavage: formation of the Co(I) intermediate in the corrinoid/iron-sulfur protein from Clostridium thermoaceticum. (sigmaaldrich.com)
  • We have completed the first direct structural characterization of an enzyme-bound four-coordinate Co(I) intermediate, in this case for the corrinoid/iron-sulfur protein (C/Fe-SP) from Clostridium thermoaceticum. (sigmaaldrich.com)
  • RA Maupin-Furlow J, Ferry JG RL J Bacteriol 1996 Jan;178(2):340-6 RN [2] RT Cloning and expression of the gene cluster encoding key proteins involved in acetyl-CoA synthesis in Clostridium thermoaceticum: CO dehydrogenase, the corrinoid/Fe-S protein, and methyltransferase. (jcvi.org)
  • in some species N 5-methyltetrahydrosarcinapterin is the methyl donor) ( Thauer 1998 ) to the corrinoid prosthetic group harbored by MtrA ( Hippler and Thauer 1999 ), a protein anchored to cell membrane ( Harms and Thauer 1996 ). (g3journal.org)
  • Corrinoids are essential cofactors for the activity of reductive dehalogenase enzymes. (wur.nl)
  • Many anaerobic bacteria can catalyze the first two reactions of this pathway using the cofactors heme, factor F 430 , and corrinoids, such as aquocobalamin and methylcobalamin. (ethz.ch)
  • Corrinoid (vitamin B 12 -like) cofactors contain various α-axial ligands, including 5,6-dimethylbenzimidazole (DMB) or adenine. (elsevier.com)
  • Wild-type cells make pseudo-B 12 cofactors during aerobic growth on propanediol plus Cbi and can use pseudo-vitamin B 12 for all of their corrinoid-dependent enzymes. (elsevier.com)
  • Each transferase catalyzes the transfer of a methyl group from mono-, di- or trimethylamine to each of their respective corrinoid cofactors, and the three methyltransferases are not all present in all methane producing organisms. (biomedcentral.com)
  • Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase. (scienceexchange.com)
  • Methanol:coenzyme M methyltransferase from Methanosarcina barkeri -- substitution of the corrinoid harbouring subunit MtaC by free cob(I)alamin. (kegg.jp)
  • Corrinoids (derivatives of vitamin B12) are an essential cofactor of reductive dehalogenases, the key enzymes involved in the environmental-friendly process of organohalide respiration (OHR). (epfl.ch)
  • Members of the seed alignment include corrinoid proteins specific to four different, mutally non-homologous enzymes of the genus Methanosarcina. (jcvi.org)
  • This process is centred around the reductive dehalogenases, an iron-sulfur and corrinoid containing family of enzymes. (ufz.de)
  • In this bacterium, CobA is the housekeeping adenosyltransferase needed for de novo biosynthesis and for the assimilation of exogenous corrinoids. (asm.org)
  • Of the liberated corrinoids, only the cobalamins attach to intrinsic factor. (veganhealth.org)
  • Vitamin B 12 (Cyanocobalamin) Vitamin B 12 refers to a group of Cobalt-containing corrinoids known as cobalamins. (expertsmind.com)
  • In the enterobacterium Salmonella enterica , corrinoid adenosylation is required for de novo synthesis and for the assimilation of incomplete precursors such as cobinamide ( 12 ). (asm.org)
  • Three-dimensional structure of ATP:corrinoid adenosyltransferase from Salmonella typhimurium in its free state, complexed with MgATP, or complexed with hydroxycobalamin and MgATP. (expasy.org)
  • The bacterium Salmonella enterica produces the corrin ring only under anaerobic conditions, but it can form "complete" corrinoids aerobically by importing an "incomplete" corrinoid, such as cobinamide (Cbi), and adding appropriate α- and β-axial ligands. (elsevier.com)
  • Corrinoids like cyanocobalamin (vitamin B 12 ) and dicyanocobinamide served as electron shuttles and as catalysts between the reducing agent (e.g., titanium (III) citrate) and the electron accepting iodinated compound. (ufz.de)
  • 000200028 001__ 200028 000200028 005__ 20180913062547.0 000200028 037__ $$aPOST_TALK 000200028 245__ $$aSalvage of the essential corrinoid cofactor in Dehalobacter restrictus 000200028 269__ $$a2014 000200028 260__ $$c2014 000200028 336__ $$aPosters 000200028 520__ $$aThe corrinoid cofactor is essential for organohalide respiration (OHR), a bacterial metabolism assisting towards environmental depollution. (epfl.ch)
  • 2003) The cofactor of tetrachloroethene reductive dehalogenase of Dehalospirillum multivorans is norpseudo‐B12, a new type of a natural corrinoid. (els.net)
  • Genomic analysis of D. restrictus revealed the presence of all the genes required for the production of corrinoids (1), however the strain is incapable of de novo biosynthesis. (epfl.ch)
  • Moreover, in proteomic data of a D. restrictus culture growing in presence of vitamin B12 in the medium, several corrinoid biosynthesis proteins were not detected arguing for specific regulation mechanisms. (epfl.ch)
  • The transport and scavenging metabolism of corrinoid by D. restrictus is now under scrutiny. (epfl.ch)
  • Although Dehalobacter restrictus depends strictly on OHR metabolism for growth, it cannot produce the essential corrinoid de novo. (epfl.ch)
  • Genome analysis of D. restrictus revealed a complete set of corrinoid biosynthesis genes with the exception of one non-functional gene (cbiH) involved in the contraction of the corrin ring, which could explain its corrinoid auxotrophy. (epfl.ch)
  • Proteomic analysis of D. restrictus revealed a strong up-regulation of two operons (#1, 2) involved in cobalt/corrinoid transport and salvaging as a response to corrinoid limitation. (epfl.ch)
  • Analysis of the corrinoids extracted from cells of these different cultures will show whether they are used as provided or D. restrictus modifies the nucleotide loop. (epfl.ch)
  • Using transcriptional analysis and shotgun proteomics, here we show corrinoid auxotrophy in Dehalobacter restrictus PER-K23T. (wur.nl)
  • A detailed analysis revealed that the cbiH gene whose product is involved in the corrin ring contraction displays a frame-shift mutation which was confirmed experimentally, suggesting that it might represent a possible checkpoint behind the corrinoid auxotrophy. (epfl.ch)
  • Insights into the corrinoid adenosylation pathway in S. enterica (Fig. 1 ) were recently reported, including the three-dimensional crystal structure of the ATP:co(I)rrinoid adenosyltransferase (CobA) enzyme responsible for the formation of the Co-C bond ( 2 , 13 - 15 , 35 ). (asm.org)
  • However, CobA is not the only corrinoid adenosyltransferase present in S. enterica . (asm.org)
  • The eutT gene of the eut operon was suggested to encode the corrinoid adenosyltransferase enzyme for this pathway, but support for this assignment was inconclusive ( 20 ). (asm.org)
  • Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a four-coordinate inter. (nih.gov)
  • However, genomic and physiological analyses of obligate OHR bacteria (such as Dehalobacter and Dehalococcoides) have delivered contrasting results on the ability of de novo corrinoid biosynthesis. (epfl.ch)
  • The general aim of the present study is to better understand the corrinoid metabolism of the genus Dehalobacter at the level of biosynthesis, regulation and transport. (epfl.ch)
  • are small to average in size (about 2.5-3 Mbp) and the genes necessary for organohalide respiration, if present, are clustered in one area, including corrinoid biosynthesis genes responsible for production of the unique norpseudovitamin B 12 . (springer.com)
  • The genome of Methanosarcina acetivorans encodes three homologs, initially annotated as hypothetical fused corrinoid/methyl transfer proteins, which are highly elevated in CO-grown cells versus cells grown with alternate substrates. (asm.org)
  • The UV-visible spectrum was typical of methylated corrinoid-containing proteins, with absorbance maxima at 370 and 420 nm and a band of broad absorbance between 450 and 600 nm with maxima at 525, 490, and 550 nm. (asm.org)
  • Hence, such OHRB incapable of de novo corrinoid synthesis will be dependent on non-dechlorinating community members to fulfill their nutritional needs indicating paramount importance of syntrophic interactions in supporting robust growth and activity of OHRB. (wur.nl)
  • To investigate the molecular basis of this requirement for heterotrophic bacteria, we performed a screen using the defined nonredundant transposon library of Vibrio cholerae, which implicated genes in corrinoid uptake and biosynthesis. (ucsd.edu)
  • RN [1] RM 96134964 RT Characterization of the cdhD and cdhE genes encoding subunits of the corrinoid/iron-sulfur enzyme of the CO dehydrogenase complex from Methanosarcina thermophila. (jcvi.org)
  • The human corrinoid binders intrinsic factor, transcobalamin, and haptocorrin recognized this corrinoid like vitamin B 12 . (asm.org)
  • A linear correlation between the rate of iodide release and the corrinoid concentration was observed, with deiodination rates for dicyanocobinamide twice as high as for vitamin B 12 . (ufz.de)
  • Vitamin B12 is a collective term for these variously substituted corrinoids. (mdpi.com)
  • Assays of vitamin B-12 in Spirulina Pacifica using the standard US Pharmacopeia (USP) method to measure total corrinoids reveals an average activity of about 7 micrograms per 3 grams of Spirulina (one serving size). (veganforum.com)
  • These figures demonstrate that about 36% of the total corrinoid vitamin B-12 activity in Spirulina is human active. (veganforum.com)
  • Corrinoid-compounds were purified and characterized from these algae to clarify the chemical properties and bioavailability of the algal vitamin B12. (biomedsearch.com)
  • Pseudovitamin B12 (an inactive corrinoid) predominated in the spirulina tablets, which are not suitable for use as a vitamin B12 source, especially for vegetarians. (biomedsearch.com)
  • Under aerobic conditions, S. enterica performs the corrinoid-dependent degradation of ethanolamine if given vitamin B 12 , but it can make B 12 from exogenous Cbi only if DMB is also provided. (elsevier.com)
  • Vitamin B12, a member of the B-vitamin family, is a term used collectively to represent a group of cobalt-containing compounds known as corrinoids. (positivearticles.com)
  • Corrinoids are a group of compounds based on the skeleton of corrin, a cyclic system containing four pyrrole rings similar to porphyrins. (wikipedia.org)
  • Two different strategies for obtaining corrinoids, are used by OHR bacteria, either de novo biosynthesis or salvaging from the environment. (epfl.ch)
  • In spite of considerable effort, there is still much controversy and uncertainty concerning the mode of action of corrinoid coenzymes. (docme.ru)
  • This raised the question of the source of corrinoids for obligate OHR bacteria in the environment and their biosynthesis/scavenging mechanism of corrinoids. (epfl.ch)
  • Here, we characterize amoebal isolate LPG3, which consumes cyanobacteria as its primary food source but also requires heterotrophic bacteria as a source of corrinoid vitamins. (ucsd.edu)
  • Amoeba LPG3 specifically requires the corrinoid variant produced by heterotrophic bacteria and cannot grow on cyanobacteria alone, as they produce a different corrinoid variant. (ucsd.edu)
  • The response of these bacteria to environmental stresses such as corrinoid starvation, increasing concentrations of ortho- and meta-substituted chlorophenols and different chlorobenzenes will be studied. (wur.nl)
  • Another important feature of corrinoid in OHR metabolism is the nature of the nucleotide loop, as illustrated by the unusual corrinoid identified in the organohalide-respiring Sulfurospirillum multivorans. (epfl.ch)
  • The corrinoid adenosylation pathway in S. enterica . (asm.org)
  • HPLC, UV-visible spectroscopy, MS, and bioassay data established adenosylcobyric as the corrinoid product of the CbiZ-catalyzed reaction. (nih.gov)
  • The structures define the corrinoid binding site and provide visual evidence for a base-off, four-coordinate cob(II)alamin intermediate. (nih.gov)
  • Other large operons like the 1,2-propanediol utilization ( pdu ) and ethanolamine utilization ( eut ) operons appear to encode their own corrinoid adenosyltransferases. (asm.org)
  • Analysis of corrinoids in ovine tissues" by R J. Kelly, Tini M. Gruner et al. (edu.au)
  • All corrinoids were present in the medium at 100 pM. (nih.gov)
  • In contrast to humans, where me-cbl constitutes the highest proportion of corrinoids in plasma and ado-cbl in the liver, in sheep the amount of ado-cbl was consistently higher than me-cbl in all tissues. (edu.au)
  • This same corrinoid specificity is also exhibited by other eukaryotes, including humans and algae. (ucsd.edu)