A noninflammatory, usually bilateral protrusion of the cornea, the apex being displaced downward and nasally. It occurs most commonly in females at about puberty. The cause is unknown but hereditary factors may play a role. The -conus refers to the cone shape of the corneal protrusion. (From Dorland, 27th ed)
The lamellated connective tissue constituting the thickest layer of the cornea between the Bowman and Descemet membranes.
The measurement of curvature and shape of the anterior surface of the cornea using techniques such as keratometry, keratoscopy, photokeratoscopy, profile photography, computer-assisted image processing and videokeratography. This measurement is often applied in the fitting of contact lenses and in diagnosing corneal diseases or corneal changes including keratoconus, which occur after keratotomy and keratoplasty.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Partial or total replacement of all layers of a central portion of the cornea.
Conferences, conventions or formal meetings usually attended by delegates representing a special field of interest.
Therapies that involve the TRANSPLANTATION of CELLS or TISSUES developed for the purpose of restoring the function of diseased or dysfunctional cells or tissues.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
The use of photothermal effects of LASERS to coagulate, incise, vaporize, resect, dissect, or resurface tissue.
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
Removal of tissue with electrical current delivered via electrodes positioned at the distal end of a catheter. Energy sources are commonly direct current (DC-shock) or alternating current at radiofrequencies (usually 750 kHz). The technique is used most often to ablate the AV junction and/or accessory pathways in order to interrupt AV conduction and produce AV block in the treatment of various tachyarrhythmias.
A surgical procedure to correct MYOPIA by CORNEAL STROMA subtraction. It involves the use of a microkeratome to make a lamellar dissection of the CORNEA creating a flap with intact CORNEAL EPITHELIUM. After the flap is lifted, the underlying midstroma is reshaped with an EXCIMER LASER and the flap is returned to its original position.
A type of refractive surgery of the CORNEA to correct MYOPIA and ASTIGMATISM. An EXCIMER LASER is used directly on the surface of the EYE to remove some of the CORNEAL EPITHELIUM thus reshaping the anterior curvature of the cornea.
Gas lasers with excited dimers (i.e., excimers) as the active medium. The most commonly used are rare gas monohalides (e.g., argon fluoride, xenon chloride). Their principal emission wavelengths are in the ultraviolet range and depend on the monohalide used (e.g., 193 nm for ArF, 308 nm for Xe Cl). These lasers are operated in pulsed and Q-switched modes and used in photoablative decomposition involving actual removal of tissue. (UMDNS, 2005)
Organizations established by endowments with provision for future maintenance.
The condition in which individuals are financially unable to access adequate medical care without depriving themselves and their dependents of food, clothing, shelter, and other essentials of living.
The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
The maximum compression a material can withstand without failure. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed, p427)
Theoretical representations that simulate the behavior or activity of the cardiovascular system, processes, or phenomena; includes the use of mathematical equations, computers and other electronic equipment.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
The properties, processes, and behavior of biological systems under the action of mechanical forces.
Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Progenitor cells from which all blood cells derive.
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
Surgical techniques on the CORNEA employing LASERS, especially for reshaping the CORNEA to correct REFRACTIVE ERRORS.
Bilateral hereditary disorders of the cornea, usually autosomal dominant, which may be present at birth but more frequently develop during adolescence and progress slowly throughout life. Central macular dystrophy is transmitted as an autosomal recessive defect.
Surgical procedures employed to correct REFRACTIVE ERRORS such as MYOPIA; HYPEROPIA; or ASTIGMATISM. These may involve altering the curvature of the CORNEA; removal or replacement of the CRYSTALLINE LENS; or modification of the SCLERA to change the axial length of the eye.
Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.
The state of being free from intrusion or disturbance in one's private life or affairs. (Random House Unabridged Dictionary, 2d ed, 1993)
The privacy of information and its protection against unauthorized disclosure.
Protective measures against unauthorized access to or interference with computer operating systems, telecommunications, or data structures, especially the modification, deletion, destruction, or release of data in computers. It includes methods of forestalling interference by computer viruses or so-called computer hackers aiming to compromise stored data.
Voluntary authorization, by a patient or research subject, with full comprehension of the risks involved, for diagnostic or investigative procedures, and for medical and surgical treatment.
The protection of genetic information about an individual, family, or population group, from unauthorized disclosure.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.
A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors.
The process by which semen is kept viable outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
Centers for storing various parts of the eye for future use.
The capability of bearing live young (rather than eggs) in nonmammalian species. Some species of REPTILES and FISHES exhibit this.
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.

Regulation of gelatinase B production in corneal cells is independent of autocrine IL-1alpha. (1/726)

PURPOSE: The matrix metalloproteinase gelatinase B is synthesized by cells at the leading edge of the corneal epithelium migrating to heal a wound. Recent data from the authors' laboratory suggest that excessive synthesis contributes to repair defects. The goal of the study reported here was to investigate mechanisms controlling gelatinase B production by corneal epithelial cells. METHODS: Freshly isolated cultures of corneal epithelial cells and early passage stromal fibroblasts from rabbit were used for these studies. RESULTS: In a previous study, it was found that the cytokine interleukin (IL)-1alpha is released into the culture medium of corneal epithelial cells more efficiently when they are plated at low density with limited cell-cell contact than when plated at high density. In this study, we show that production of gelatinase B by these cells is similarly affected by cell plating density. However, it is further demonstrated that these two events are not dependent on one another but occur in parallel: IL-1alpha does not regulate gelatinase B production (synthesis), nor was there evidence that any other secreted autocrine cytokine acts as mediator. Instead, our data suggest that gelatinase B production is downregulated directly by high cell density and indicate a connection to the level of protein kinase C activity. Nevertheless, the anticancer agent suramin, which blocks collagenase synthesis by interfering with autocrine cytokine-receptor interactions, still inhibits synthesis of gelatinase B. CONCLUSIONS: Unlike collagenase synthesis by corneal stromal fibroblasts, production (synthesis) of gelatinase B does not appear to be controlled by secreted autocrine cytokines but can still be inhibited by suramin. Suramin may make an effective therapeutic agent for controlling pathologic overproduction of gelatinase B in corneal ulcers.  (+info)

Human corneal ablation threshold using the 193-nm ArF excimer laser. (2/726)

PURPOSE: To determine the human corneal threshold ablation energy density for the 193-nm ArF excimer laser, approximating clinical conditions. METHODS: The VISX Star (Santa Clara, CA) 193-nm argon fluoride excimer laser was used to ablate the cornea in human eye bank eyes under clinical conditions. Corneas were exposed to energy densities of 10, 20, 30, 35, 40, 45, and 140 to 160 mJ/cm2. Corneas were fixed for light and transmission electron microscopy immediately after laser exposure. RESULTS: Different ablation thresholds for various corneal structural elements were observed. The ablation threshold for the collagen in the corneal stroma was determined to be 30 mJ/cm2. Keratocytes had ablation thresholds of 40 mJ/cm2. These different ablation thresholds accounted for the production of stromal peaks and valleys, with the keratocytes atop the peaks. CONCLUSIONS: Different corneal structural elements have different ablation threshold energy densities.  (+info)

Differential inhibition of collagenase and interleukin-1alpha gene expression in cultured corneal fibroblasts by TGF-beta, dexamethasone, and retinoic acid. (3/726)

PURPOSE: Expression of the genes for collagenase and interleukin-1alpha (IL-1alpha) are induced as stromal cells become activated to the repair fibroblast phenotype after injury to the cornea. This investigation examines the mechanisms whereby expression of these genes is inhibited by transforming growth factor-beta (TGF-beta), dexamethasone (DEX), or retinoic acid (RET A). METHODS: A model of freshly isolated cultures of corneal stromal cells and early passage cultures of corneal fibroblasts was used in these studies. This model reproduces the events of stromal cell activation in the corneal wound. RESULTS: In early passage cultures of corneal fibroblasts, expression of collagenase is under obligatory control by autocrine IL-1alpha. IL-1alpha controls its own expression through an autocrine feedback loop that is dependent on transcription factor NF-kappaB. TGF-beta, DEX, and RET A were each effective inhibitors of collagenase gene expression in these cells. Furthermore, these agents have the capacity to inhibit expression of IL-1alpha and this was correlated with their ability to affect DNA-binding activity of NF-kappaB. However, TGF-beta, DEX, and RET A were also effective inhibitors of the low level of collagenase expressed by freshly isolated corneal stromal cells that cannot express IL-1alpha. CONCLUSIONS: In cells with an active IL-1alpha autocrine loop there are at least two distinct signaling pathways by which collagenase gene expression can be modulated. The results of this study demonstrate that TGF-beta, DEX, and RET A differentially inhibit collagenase and IL-1alpha gene expression. This information will be useful in the design of therapeutic modalities for fibrotic disease in the cornea and other parts of the eye.  (+info)

A new surgical technique for deep stromal, anterior lamellar keratoplasty. (4/726)

AIMS: To describe a new surgical technique for deep stromal anterior lamellar keratoplasty. METHODS: In eye bank eyes and sighted human eyes, aqueous was exchanged by air, to visualise the posterior corneal surface--that is, the "air to endothelium" interface. Through a 5.0 mm scleral incision, a deep stromal pocket was created across the cornea, using the air to endothelium interface as a reference plane for dissection depth. The pocket was filled with viscoelastic, and an anterior corneal lamella was excised. A full thickness donor button was sutured into the recipient bed after stripping its Descemet's membrane. RESULTS: In 25 consecutive human eye bank eyes, a 12% microperforation rate was found. Corneal dissection depth averaged 95.4% (SD 2.7%). Six patient eyes had uneventful surgeries; in a seventh eye, perforation of the lamellar bed occurred. All transplants cleared. Central pachymetry ranged from 0.62 to 0.73 mm. CONCLUSION: With this technique a deep stromal anterior lamellar keratoplasty can be performed with the donor to recipient interface just anterior to the posterior corneal surface. The technique has the advantage that the dissection can be completed in the event of inadvertent microperforation, or that the procedure can be aborted to perform a planned penetrating keratoplasty.  (+info)

Proteoglycan synthesis by bovine keratocytes and corneal fibroblasts: maintenance of the keratocyte phenotype in culture. (5/726)

PURPOSE: To determine the effect of serum on morphology, growth, and proteoglycan synthesis by primary cultures of collagenase-isolated bovine keratocytes. METHODS: Keratocytes were isolated from bovine corneas using sequential collagenase digestion and cultured in Dulbecco's modified Eagle's medium (DMEM), with and without fetal bovine serum (FBS). Proteoglycans synthesized by the cells in culture and by keratocytes in intact cornea culture were metabolically radiolabeled with 35SO4. The proteoglycans were characterized by their sensitivity to keratanase, chondroitinase ABC, and heparatinase and by their size on Superose 6 HR. Cell number was determined by measuring DNA content of the culture dishes. RESULTS: Keratocytes cultured in 10% FBS proliferated, appeared fibroblastic, and synthesized only 9% of the total glycosaminoglycan as keratan sulfate (KS), whereas cells in serum-free media were quiescent, appeared dendritic, and synthesized 47% KS, a value similar to the 45% KS for corneas radiolabeled overnight in organ culture. This increased proportion of KS synthesis in serum-free media was caused by a moderate increase in KS synthesis combined with a substantial decrease in chondroitin sulfate (CS) synthesis. Fractionation on Superose 6 High Resolution showed the size and relative amounts of the CS- and KS-containing proteoglycans synthesized by keratocytes in serum-free media also more closely resembled that of keratocytes in corneas in organ culture than keratocytes in media containing serum. CONCLUSIONS: A comparison of proteoglycan synthesis and cell morphology between keratocytes in corneas in organ culture and in cell culture indicates that keratocytes maintain a more native biosynthetic phenotype and appearance when cultured in serum-free media. These results also suggest that culturing in the presence of serum fundamentally alters the keratocyte phenotype to an activated cell, mimicking certain changes observed during wound healing.  (+info)

Failure to activate transcription factor NF-kappaB in corneal stromal cells (keratocytes). (6/726)

PURPOSE: Freshly isolated cultures of corneal stromal cells (keratocytes) are incompetent to synthesize the tissue remodeling proteinase, collagenase, in response to agents such as cytochalasin B (CB) or phorbol myristate acetate (PMA), which are strong stimulators of collagenase expression in subcultured fibroblasts of all types, including those from corneal stroma. Incompetence is due to failure to activate an autocrine interleukin (IL)1alpha feedback loop required to mediate cell response. The goal of the present study was to investigate the mechanism for this failure. METHODS: A cell culture model of freshly isolated corneal stromal cells and subcultured stromal fibroblasts from rabbits was used for these studies. RESULTS: Competence to synthesize collagenase in response to CB was acquired as a differentiation property by corneal stromal cells placed in culture, and did not require subculture. Competence acquisition correlated with transition to a fibroblastic spindle shape, assembly of actin stress fibers, and the acquired capacity to collapse in response to CB. It was demonstrated that competence could be more precisely defined as the capacity to express IL-1alpha in response to IL-1, making possible activation of the feedback loop. Investigation into the signaling pathway for IL-1alpha expression in response to IL-1 revealed a requirement for reactive oxygen species and activity of the transcription factor nuclear factor (NF)kappaB. Importantly, freshly isolated stromal cells were found to be relatively incompetent to activate NF-kappaB in comparison to subcultured stromal fibroblasts. CONCLUSIONS: Failure to activate NF-kappaB explains incompetence for expression of IL-1alpha in corneal stromal cells. Because NF-kappaB regulates many cell functions with potential to disturb corneal structure, including expression of inflammatory, stress, and degradative proteinase genes; protection against apoptosis; and cell replication; this seems likely to be an important mechanism protecting corneal stasis and preserving function.  (+info)

Functional human corneal equivalents constructed from cell lines. (7/726)

Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological, biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked human corneas in key physical and physiological functions, including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.  (+info)

Distribution of ascorbate in the anterior bovine eye. (8/726)

PURPOSE: To analyze the ascorbate distribution in the anterior eye wall to better understand the functional significance of this compound in the eye. METHOD: Ascorbic acid was determined by high-performance liquid chromatography using an LC-10 system (Shimadzu, Kyoto, Japan). Bovine eye samples were used. RESULTS: The highest ascorbate concentration was observed in the corneal epithelium, with significantly higher values in the central (1.56 mg/g) than in the peripheral (1.39 mg/g) area. The ascorbate content was similar in the corneal stroma (0.22 mg/g), the Descemet's membrane (DM)/endothelium (0.22 mg/g), and the aqueous humor (0.21 mg/ml). By comparison, the sclera (0.15 mg/g) and the conjunctiva (0.11 mg/g) showed lower values, as did the lacrimal gland (0.09 mg/g) and the serum (0.0008 mg/ml). CONCLUSIONS: (1) Peak ascorbate concentration was observed in the central corneal epithelium covering the pupillary area. This is compatible with the idea that the ascorbate may act as an UV filter shielding internal eye structures from radiation damage. (2) The ascorbate concentration in the corneal stroma and DM/endothelium was as high as in the aqueous humor, and it is suggested that the aqueous humor plays a key role in the distribution of ascorbate to the anterior eye wall.  (+info)

TY - JOUR. T1 - Quantitative assessment of anteroposterior keratocyte density in the normal rabbit cornea. AU - Petroll, W. M.. AU - Boettcher, K.. AU - Barry, P.. AU - Cavanagh, D.. AU - Jester, J. V.. PY - 1995. Y1 - 1995. N2 - The anteroposterior keratocyte density distribution in the rabbit cornea was measured. Unsectioned tissue blocks from the central cornea of five rabbits were stained with propidium iodide and imaged using a Leica laser scanning confocal microscope. A z-series of images was acquired in each sample, from anterior to posterior stroma in either 3- or 8-μm steps. Software was developed to allow interactive marking of the keratocyte nuclei within each section of the z-series and for calculating cell density. For convenience, cell density was expressed as the number of cells per corneal volume element (CVE), where CVE is a newly defined volume unit with x, y, and z dimensions of 250, 250, and 10 μm, respectively. The calculated keratocyte density was 20.2 ± 1.0 cells/CVE (n ...
The expression of keratocyte- and/or myofibroblast-specific markers and the involvement of VSX1 in wound-healing responses was further investigated in normal and wounded corneas. As expected, wounded corneas showed positive VSX1 staining in the corneal stroma, whereas no signal was observed in normal tissues (Fig. 5) . When VSX1 expression was correlated with CD-34 (keratocyte-specific marker) and α-SMA (myofibroblast-specific marker) expression in normal or wounded corneas, we observed that VSX1 was strongly associated with upregulation of α-SMA and downregulation of CD-34. Normal corneas typically characterized by a quiescent keratocyte population showed high CD-34 (Fig. 6I)expression and barely detectable α-SMA (Fig. 6E)expression, but did not express VSX1 (Fig. 6A) , which is consistent with the presence of a quiescent keratocyte population. In wounded corneas (n = 3) VSX1 expression was strictly correlated with that of α-SMA. Corneas exhibiting increasing values of α-SMA (Figs. 6E 6F ...
The substantia propria (or stroma of cornea) is fibrous, tough, unyielding, and perfectly transparent. At its centre, human corneal stroma is composed of about 200 flattened lamellæ (layers of collagen fibrils), superimposed one on another.[1] They are each about 1.5-2.5 μm in thickness. The anterior lamellæ interweave more than posterior lamellæ. The fibrils of each lamella are parallel with one another, but at different angles to those of adjacent lamellæ. The lamellæ are produced by keratocytes (corneal connective tissue cells), which occupy about 10% of the substantia propria. Apart from the cells, the major non-aqueous constituents of the stroma are collagen fibrils and proteoglycans. The collagen fibrils are made of a mixture of type I and type V collagens. These molecules are tilted by about 15 degrees to the fibril axis, and because of this, the axial periodicity of the fibrils is reduced to 65 nm (in tendons, the periodicity is 67 nm). The diameter of the fibrils is remarkably ...
Purpose : Upon injury to the cornea, the composition of the extracellular matrix (ECM) rapidly changes to promote wound healing through its interactions with integrins. We hypothesize that ECM remodelling occurring during corneal wound healing causes the activation of very specific signal transduction mediators that favor faster closure of the wound. Our goal is to proceed to the pharmacological inhibition and/or activation of the PI3K/Akt mediators Akt and CREB using the human tissue-engineered cornea (hTECs) as a model. Methods : hTECs produced by the self-assembly approach were wounded with a 8-mm diameter biopsy punch and deposited on another reconstructed human corneal stroma to allow wound closure on a natural ECM. Total RNAs and proteins were prepared from the epithelial cells of wounded and unwounded areas and their gene expression pattern was determined by microarrays. The wounded tissues were then incubated with or without C646 (a CREB inhibitor) or with or without SC79 (an AKT ...
De-epithelialised and de-endothelialised bovine corneal stromas with a hydration of 3.2 equilibrated at 154 mM NaCl and buffered at pH 7.4 had their optical density (400-750 nm) measured. Stromas equilibrated against 10, 20, 30, 50 or 100 mM NaCl made isotonic to 154 mM NaCl by supplementing with sorbitol were progressively more transparent as NaCl increased. Hypertonic equilibration against 300, 600 or 1000 mM NaCl resulted in a progressive loss of transparency compared with 154 mM NaCl. Light scattering as a function of wavelength fitted a l_3 function well for 10, 30, 50, 100 and 154 mM NaCl preparations between 450 and 650 nm, but not at higher wavelengths. However, hypertonic 300, 600 and 1000 mM NaCl preparations showed a l_2 dependence in the 450-750 nm range. Experiments with 154 mM NaCl and either 0 or 300 mM sorbitol suggested that the changes in light scattering in hypertonic preparations are unlikely to be caused by osmotic alterations to the stromal keratocytes. Psychophysical ...
Figure 5. TEM of collagen fibrils from mouse corneal stroma. Transmission electron micrographs containing cross-sections of collagen fibrils from corneal stroma from wild-type (A) and mutant (C) mice. Morphometric analysis of corneal collagen fibrils in wild-type (B) and in mutant (D) mice. Fibril diameter was measured as described in methods and presented in a histogram. The average fibril diameter, the variance and the total number (n) of fibrils measured are indicated.. ...
The laboratory mouse is an unsurpassed animal model for studying human diseases due to the abundant availability of mutant species, and the close homology of the human and mouse genomes [14,15]. The physical dimensions of the mouse eye and resulting difficulty in performing manual surgery using conventional instrumentation however complicates accurate ocular surgical models in this animal. In this study we have demonstrated that it is possible to adapt and use a conventional clinical femtosecond laser platform to perform intrastromal lamellar surgery on the mouse eye. The FSL laser causes tissue photo-disruption, which results in the formation of plasma that rapidly expands and collapses, to create an interlamellar (collagen lamellae) cavitation at a precise depth [16]. In the TEM analyses of these cavitations in the mouse cornea we were able to demonstrate distinct localized plasma cavitations with dimensions of ~1 µm. The size of these plasma cavitations is in part related to the energy ...
Data Availability StatementThe datasets used and/or analyzed during the current study are available from your corresponding author on reasonable request. were prepared and subsequently stained with eosin and hematoxylin or antibodies for immunofluorescence observation of human being corneal stroma-related proteins. Results SP marketed the appearance of corneal stroma-related collagens (collagen types I, III, V, and VI) through the differentiation induced by KDM. Patterned silk membrane led cell position of PDLSCs, and essential ECM the different parts of the corneal stroma had been been shown to be transferred with the cells. The built multi-lamellar tissues was found to aid cells developing between every two levels and expressing the primary kind of collagens (collagen types I and V) and proteoglycans (lumican and keratocan) of Clofarabine price Clofarabine price regular individual corneal stroma. Conclusions Multi-lamellar individual corneal stroma-like tissues could be built in vitro by ...
C: Stromal thickness of the Fb was found to be significantly smaller than for ASC in presence of urothelial cells. The UTS of the Fb group was significantly higher compared to the ASC. The failure strains were not significantly different between the two constructions. Tests were performed using 3 different cellular populations (N) for Fbs and ASCs and each construct was produced in triplicate (n). Each column represents mean +/-standard error of the mean, with p,0.05 indicating significance (*p,0.05, ** p,0.005 ...
Herpetic stromal keratitis (HSK) is an immunopathological and tissue destructive corneal lesion caused by herpes simplex virus (HSV) infection, which induces an intense inflammatory response and finally leads to blindness. Accumulating evidence using the murine model has shown that Th-1 phenotype CD4+ T cells orchestrating the inflammation mainly contribute to the immunopathological reaction in HSV-1 infected cornea. However, prior to CD4+ T cell infiltration into corneal lesions, various innate immune cells recruit and produce numerous inflammatory and angiogenic molecules into the corneal stroma those in turn drive the corneal immunopathology. The first part (Part I) of this dissertation focuses on the understanding of HSV-1 induced immunoinflammatory processes in the cornea and trigeminal ganglia including the secondary lymphoid tissues. The next three parts (Part II-IV) focus on different inflammatory and anti-inflammatory mechanisms that are activated following virus infection in the
After corneal injury, keratocytes become activated and transform into repair phenotypes-corneal fibroblasts or myofibroblasts, however, these important cells are difficult to identify histologically, compromising studies of stromal wound healing. Recent studies indicate that expression of the cell surface protein, Thy-1, is induced in fibroblast populations associated with wound healing and fibrosis in other tissues. We investigated whether keratocyte transformation to either repair-associated phenotype induced Thy-1 expression. Human corneal keratocytes were isolated by collagenase digestion. The cells were either processed immediately (i.e. freshly isolated keratocytes) or were cultured in the presence of 10% fetal bovine serum or transforming growth factor-β to induce transformation to the corneal fibroblast and myofibroblast phenotypes, respectively. Thy-1 mRNA and protein expression by freshly isolated keratocytes and corneal fibroblasts were assessed by RT-PCR and Western blotting. mRNA ...
3DBT corneas are comparable to human donor corneas 3DBT corneas are made from human cells, without artificial materials or scaffolds They have the same physical characteristics as the human corneal stroma They can be seen as a direct replacement to donor tissue for anterior keratoplasty
Most studies of herpetic corneal disease use a primary infection model. However, primary infection with HSV-1 does not typically lead...
We measure the surface ablation threshold fluence of porcine corneal stroma for 100 fs laser pulses, with wavelengths between 800 nm and 1450 nm, generated by a Ti:sapphire-pumped optical parametric amplifier. The ablation threshold was found to vary only slightly within this wavelength range, between 1.5 and 2.2 J/cm2. The data suggest a rapid increase of the ablation threshold for wavelengths up to about 1000 nm, followed by a plateau for longer wavelengths. This behavior is partly confirmed by a simple theoretical model of the ablation process. The influence of the wavelength on the physics of surface ablation is discussed on the basis of the model.. ©2008 Optical Society of America. Full Article , PDF Article ...
Corneal collagen crosslinking (CXL) is a minimally invasive procedure that is currently supposed to arrest the progression of corneal ectatic conditions. CXL induces crosslinks in the stromal collagen of the cornea.
An electromechanical model for charged, hydrated tissues is developed to predict the kinetics of changes in swelling and isometric compressive stress induced by changes in bath salt concentration. The model focuses on ionic transport as the rate limiting step in chemically modulating electrical interactions between the charged macromolecules of the extracellular matrix. The swelling response to such changes in local interaction forces is determined by the relative rates of chemical diffusion and fluid redistribution in the tissue sample. We have tested the model by comparing the experimentally observed salt-induced stress relaxation response in bovine articular cartilage and corneal stroma to the response predicted by the model using constitutive relations for the concentration dependent material properties of the tissues reported in a related study. The qualitatively good agreement between our experimental measurements and the predictions of the model supports the physical basis of the model ...
Stromal swelling in human, cat, and rabbit cornea is biphasic, interpretable as an elementary cusp catastrophe proposed by Thom, with t* ═ log t and Q* ═ log Q (stromal charge Q, time t) as control parameters, and H0.5 (hydration H) as the state variable. A thermodynamic potential with two attractor regions, each with a local minimum, governs corneal stromal swelling. Transitions follow a saturation convention whereby the second minimum is preferred upon availability. Corneal swelling is an example of a space-equivalent unfolding, where the transition plane moves in time. It is proposed that the transition plane coincides with the uncoupling of interfibrillary linkages or springs in the corneal stroma, and is associated with a critical hydration of ca. 10 kg H2O per kilogram dry mass, and stromal charge ca. 1 x 10-7 mol electrons. ...
This study evaluates the intrastromal correction of ametropia with a femtosecond laser made by 20/10 PERFECT VISION. This laser generates a beam of ultrashort, infrared pulses which enables very precise cuts in the cornea. By these cuts lamellae of the cornea are separated locally, and in the consequence the curvature of the cornea is changed, and the correction of the diagnosed ametropia can be achieved. On the contrary to cuts which are generated with a sharp knife, the cuts generated when using a laser can be generated just inside the cornea without opening the surface of the cornea. This means the procedure is minimal-invasive.. The study hypothesis is: Different types of ametropia can be corrected safely and on long-term by intrastromal cuts. ...
Xiao-Tang Yin is the author of these articles in the Journal of Visualized Experiments: Recurrent Herpetic Stromal Keratitis in Mice, a Model for Studying Human HSK, Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation
With regard to TIMP 3, the total amount of this protein associated with the matrices of confluent stromal cell cultures of normal corneas maintained over a period of time of 8e10 days was approximately 5-fold higher than that within their regularly obtained culture media examples. After infecting stromal cells with RAdTIMP 3 very little of the freshly synthesised TIMP 3 was recovered in their culture media but the quantity connected with the matrices, which was measured 13 days after infection, was significantly more than normally present. Normal corneal stromal cell cultures, when 70-80 Cabozantinib price confluent infected with RAdTIMP 3, all showed symptoms of cell death between day 2 and 5 after infection. As well as the appearance of detached cells in the growth medium, significant pockets developed. As shown in Fig. 3a, these were without both cells and matrix and, because of the unusually dense packing of cells round the holes, appeared to be due to matrix contraction. Eventually ...
The Lasik flap is both an advantage and a disadvantage. Without the Lasik flap (PRK) you have no possibility of a Lasik flap related problem during surgery or your lifetime. Even though Lasik flap problems are relatively rare, no possibility is almost always better than low probability ...
A microkeratome is a precision surgical instrument with an oscillating blade designed for creating the corneal flap in LASIK or ALK surgery. The normal human cornea varies from around 500 to 600 micrometres in thickness; and in the LASIK procedure, the microkeratome creates a 100 to 200 micrometre thick flap ...
There were no statistically significant differences in the thicknesses of pre-ocular tear film, corneal epithelium, Bowman layer, corneal stroma, and Descemets membrane-endothelium complex in the myopic patients and controls (p >0.05). The anterior scleral thickness values of 1 mm, 3 mm, and 5 mm from the limbus were similar in both the study and control groups (p >; 0.05). There were no significant correlations between central cornea stromal thickness and anterior scleral thickness for all the participants (p >0.05 ...
This presentation is from the session SYM-104: Keratoconus: Consensus and Controversy from the 2019 ASCRS Annual Meeting held on May 4-7, 2019 in San Diego, California.. ...
The cornea of the eye is a unique, transparent connective tissue. It is comprised predominantly of collagen fibrils, remarkably uniform in diameter and regularly spaced, organized into an intricate lamellar array. Its establishment involves a precisely controlled sequence of developmental events in which the embryonic cornea undergoes major structural transformations that ultimately determine tissue form and function. In this article, we will review corneal developmental dynamics from a structural perspective, consider the roles and interrelationships of collagens and proteoglycans, and comment on contemporary concepts and current challenges pertinent to developmental processes that result in an optically clear, mature cornea. Developmental Dynamics 237:2607-2621, 2008. © 2008 Wiley-Liss, Inc. ...
It is well understood by oncologists that tumor growth and metastasis depend on changes in the tumor microenvironment or stroma. Stromal changes have been the focus of numerous research publications and have led to insights in both tumor development and promising new avenues for treatment [1-7].. In order to study molecular changes in stroma from tissue samples, it is necessary to separate tumor tissue from stromal tissue. Without this separation we have sample heterogeneity, which is well known to severely limit the conclusions that can be made about the specificity of molecular changes and their biological causes [5, 7-11]. This separation can be difficult in contexts where tumors are small or not well differentiated. For example, in mouse tumor xenograft models, human cancer cells are grown in immune-suppressed mice [12-15]. These models are popular in oncologic research for studying mechanisms of tumor growth and metastasis, as well as drug response. In such studies, secondary tumors (at ...
Intrastromal Rings are inserted inside the cornea to support it. It helps to restore cornea & reduces keratoconus progress. NMC Eyecare offers this treatment.
Lamella of keratocytes generate a small rearward traction force against the micromachined substratum. Bar, 5 μm. Once the lamella of a keratocyte is over the m
Increased mammographic density is associated with increased incidence of breast cancer, and is largely attributed to increased levels of stromal collagen and ch...
Guertin, Inaba T, Propranolol gagueira S, et al. Evidence brought forward at the disciplinary hearing in Lon- don in June 1992 concluded that the same individual probably had provided the samples and that an opportunity had existed for the athletes to catheter- propranolol gagueira urine from another person prior to the sample collection. (a) Immunoglobulin deposits in the corneal stroma appear as red-staining, fusiform structures.
Scientists have developed a new technique to regrow human corneas. Using key tracer molecules, researchers have been able to hunt down elusive cells in the eye
Why are pancreatic tumors so resistant to treatment? One reason is that the wound-like tissue that surrounds the tumors, called stroma, is so dense, likely preventing cancer-killing drugs from reaching the tumor. A team has now discovered heterogeneity in the fibroblast portion of the stroma, opening up the possibility of targeted treatment.
Cornea , Cornea , کتابخانه دیجیتالی دانشگاه علوم پزشکی و خدمات درمانی شهید بهشتی
கருவிழிப் படலம் அல்லது விழி வெண்படலம் (cornea) என்பது கண்ணில் ஒளி ஊடுருவக்கூடிய வட்டவடிவ முன்பகுதியாகும்.[1]விழிவெண்படலத்தில் இரத்த நாளங்களோ அல்லது நிணநீர் நாளங்களோ கிடையாது. இதனால் இரத்த ஓட்டம் இல்லாத இந்த விழி வெண்படலத்திற்கு தேவைப்படும் ஆக்சிசன் மற்றும் உணவுப்பொருட்களை கண்ணின் நடு இரத்த நாள அடுக்கான விழியடி கரும்படலத்தின் முன் பகுதியில் இருக்கும் சிலியரி ...
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The human cornea contains stem cells that can be induced to express markers consistent with multipotency in cell culture; however, there have been no studies demonstrating that human corneal keratocytes are multipotent. The objective of this study is to examine the potential of human fetal keratocytes (HFKs) to differentiate into neural crest-derived tissues when challenged in an embryonic environment. HFKs were injected bilaterally into the cranial mesenchyme adjacent to the neural tube and the periocular mesenchyme in chick embryos at embryonic days 1.5 and 3, respectively. The injected keratocytes were detected by immunofluorescence using the human cell-specific marker, HuNu. HuNu-positive keratocytes injected along the neural crest pathway were localized adjacent to HNK-1-positive migratory host neural crest cells and in the cardiac cushion mesenchyme. The HuNu-positive cells transformed into neural crest derivatives such as smooth muscle in cranial blood vessels, stromal keratocytes, and ...
Cellular therapy of the corneal stroma, with either ocular or extraocular stem cells, has been gaining a lot of interest over the last decade. Multiple publications from different research groups are showing its potential benefits in relation to its capacity to improve or alleviate corneal scars, improve corneal transparency in metabolic diseases by enhancing the catabolism of the accumulated molecules, generate new organized collagen within the host stroma, and its immunosuppressive and immunomodulatory properties. Autologous extraocular stem cells do not require a healthy contralateral eye and they do not involve any ophthalmic procedures for their isolation. Mesenchymal stem cells have been the most widely assayed and have the best potential to differentiate into functional adult keratocytes in vivo and in vitro. While embryonic stem cells have been partially abandoned due to ethical implications, the discovery of the induced pluripotent stem cells (iPSC) has opened a new and very promising field for
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On June 21, 2002, uneventful LASIK was performed OS first followed by OD seven days later on June 28, 2002. There were no intraoperative complications. Excimer laser ablation was performed with a VISX Star S3 IR Excimer Laser System (VISX Technology, California) after a superiorly hinged flap was made with a Hansatome microkeratome (Bausch & Lomb, Rochester, NY) using a 9.5-mm suction ring. A 160 micron Hansatome head was used being that it provided the thinnest available cut at the time of surgery. Intraoperative pachymetry was performed after lifting the flap, taking 3 central measurements, and recording the lowest. The residual stromal bed was calculated by obtaining the lowest central pachymetry and deriving the ablation depth from the laser. In this patient, the targeted flap thickness of 160 micrometers resulted in a measured flap thickness of 124 micrometers OD and 153 micrometers OS, thinner than the corresponding settings on the microkeratome. Hence, the calculated residual stromal bed ...
On June 21, 2002, uneventful LASIK was performed OS first followed by OD seven days later on June 28, 2002. There were no intraoperative complications. Excimer laser ablation was performed with a VISX Star S3 IR Excimer Laser System (VISX Technology, California) after a superiorly hinged flap was made with a Hansatome microkeratome (Bausch & Lomb, Rochester, NY) using a 9.5-mm suction ring. A 160 micron Hansatome head was used being that it provided the thinnest available cut at the time of surgery. Intraoperative pachymetry was performed after lifting the flap, taking 3 central measurements, and recording the lowest. The residual stromal bed was calculated by obtaining the lowest central pachymetry and deriving the ablation depth from the laser. In this patient, the targeted flap thickness of 160 micrometers resulted in a measured flap thickness of 124 micrometers OD and 153 micrometers OS, thinner than the corresponding settings on the microkeratome. Hence, the calculated residual stromal bed ...
TY - JOUR. T1 - Tissue-derived biological particles restore cornea properties in an enzyme-mediated corneal ectatic model. AU - Yin, Hongbo. AU - Wang, Xiaokun. AU - Majumdar, Shoumyo. AU - Sohn, Jeeyeon. AU - Kim, Byung Jin. AU - Stark, Walter Jackson. AU - Elisseeff, Jennifer H.. PY - 2019/12. Y1 - 2019/12. N2 - Purpose: To investigate the impact of tissue derived biological particles on enzyme-mediated weakened corneas. Methods: Rabbit corneas were treated with enzymes to create an ex vivo ectatic model that simulated representative characteristics of keratoconus (KC). Porcine cornea, cartilage, and lymph node tissues were processed to remove most cellular components and cryomilled into microparticles. The KC corneas were cultured in medium containing the tissue-derived biological particles (TDP) overnight. The mechanical, thermal, ultrastructural changes, and gene expressions of corneal stromal cells were characterized to evaluate the effects of the TDP treatment. Results: The enzyme ...
Corneal tattooing is a technique that has been used for centuries to improve either cosmesis or clinically significant anomalies.1 These anomalies include diplopia, glare and halos secondary to peripheral iridotomies (PI), and sector or total iris defects. The most commonly performed tattoo techniques are either by transepithelial intrastromal micropuncture or tattooing the anterior stroma after epithelial debridement.1 2 However, these techniques are known to be associated with several complications, including intra-operative microperforations, recurrent erosions, pigment non-homogeneity and fading of pigment over time.1 2 A newer procedure has been described, termed the intrastromal lamellar pocket technique. Several studies have investigated the use of this technique for aesthetic purposes in non-seeing … ...
The cornea is the primary refractive element of the eye and is also fundamental to the protection of the visual system. Collagen is the major constituent of the cornea, where it is organised in a lattice that enables corneal transparency. Proteoglycan macromolecules are thought to regulate the diameter and spatial order of collagen fibrils in the cornea, which are both pre-requisites for corneal transparency, although the mechanisms by which they organise fibrils are not fully elucidated. This investigation examined the morphology, morphometry and organisation of proteoglycans three-dimensionally, in both normal and genetically altered mouse corneas, to gain a greater understanding of proteoglycan structure-function relationships. In summary, we found that proteoglycans are primarily responsible for the remarkable collagen organisation in the mouse cornea, which allows for corneal transparency. The self- association of proteoglycans into complexes is likely to result in a robust attachment of ...
The cornea is made up of highly organized group of cells and proteins which are arranged in five layers; 1) the corneal ephithelium, which is a multicellular tissue layer of fast-growing and easily-regenerated cells; 2) Bowmans layer, a tough layer which protects the corneal stroma, consisting of irregularly-arranged collagen fibers; 3) the corneal stroma, which is a thick, transparent middle layer, consisting of regularly-arranged collagen fibers along with sparsely populated keratocytes; 4) Descemets membrane, which is a thin but strong sheet of tissue that serves as a protective barrier against infection and injuries; 5) endothelium, the extremely thin, innermost layer of the cornea, consisting of endothelial cells that are essential in keeping the cornea clear. ...
PURPOSE. The authors investigated the phenotypic characteristics of basally located transitional cells in peripheral superior cornea, characterized previously by their coexpression of cytokeratin 19 and vimentin and their negativity for AE5. METHODS. Twenty adult human corneas were studied, using in situ immunohistochemical techniques and a panel of specific monoclonal antibodies against various surface and cytoplasmic molecules. RESULTS. The transitional cells shared staining characteristics with limbal basal cells in their expression of alpha 6 beta 4-integrin, metallothionein, AE1, and transferrin receptor. CONCLUSIONS. These transitional epithelial cells exhibit a unique phenotype differing from that of the surrounding basal epithelial cells in the peripheral cornea but analogous to that of limbal basal cells. These findings further corroborate the hypothesis that, at least from an immunohistochemical point of view, transitional cells in the superior peripheral cornea exhibit stem cell ...
If you or someone close to you has been diagnosed with Keratoconus, you have probably done what most patients have done in the last 10 years. Googled it! When most patients look up their options online, they stumble across a million articles about corneal collagen crosslinking otherwise known as CXL. Now, luckily, the FDA has… Read More » ...
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ReLEx SMILE - Most Advanced Laser Eye Treatment, Beyond LASIK +ACI-Get Rid of your Spectacles in 15 Mins, only at Lotus Eye+ACI ReLEx SMILE (Refractive Lenticule Extraction, SMall Incision Lenticule Extraction) is a minimally invasive approach with advantage of 100+ACU bladeless form of laser eye surgery which does not require
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Magnification: 20X. Sponsored by Zeiss and awarded to an image created using one of its instruments, the award goes to an image by postdoctoral fellow Sarah Robertson and undergraduate researcher Johnson Thai, both from the lab of Dr. Sophie Deng.. The cornea is the transparent front part of the eye that covers the iris and pupil. When the middle layer of the cornea, the cornea stroma, is wounded, the healing process often causes corneal scars that lead to vision impairment. A corneal scratch is seen here (in black) at the center of the image. Collagen 1 (in green), a key player in wound healing, can be seen surrounding the nuclei of cells closest to the scratch.. The Deng lab studies the proteins that are involved in corneal stroma wound healing. They replicate the cellular processes that occur following corneal injury by growing corneal stroma fibroblasts in lab dishes and then scratching them to induce a wound. These studies are being done to inform the development of methods to regenerate ...
If you have had Intacs® and are interested in further options to improve your vision, Dr. Brian, who is one of the most experienced Keratoconus doctors, routinely evaluates patients who have had Intacs® placed elsewhere. Further vision improvement can be an option through 1) Intacs® adjustments for different size, 2) removal (also known as explant) of the upper Intac segment, 3) Holcomb C3-R®, also called corneal crosslinking, to stabilize Keratoconus, and 4) CK (conductive keratoplasty) to improve astigmatism.. If you are considering having Intacs® done by a surgeon other than Dr. Brian, here are some compelling reasons you want to re-think that and have Dr. Brian perform your Intacs®.. ...
Ocular infection with herpes simplex virus 1 (HSV-1) can result in a chronic immuno-inflammatory lesion that is a significant cause of human blindness. IFN-γ producing CD4 + T cells are generally considered the main orchestrators, and lesions are more severe if the regulatory T cell (Treg) response is compromised. Tregs have been shown to lose FoxP3 (Treg lineage factor) and adopt alternate lineage fates in a changing cytokine environment. Moreover, little is known about the stability of Treg cells in an ongoing inflammatory reaction such as is the case of SK. In this study-using fate mapping mice, we were able to demonstrate that the population of ex-Tregs increased at the site of infection that is the cornea and also at the secondary lymphoid organs after HSV-1 infection. In vivo studies showed that these ex-Tregs acquired the Th1 and Th17 phenotype, which may play a destructive role in stromal keratitis. We also demonstrate in vivo that CD25lo subset of Tregs is less suppressive and more ...
CXL is a unique treatment because it is noninvasive and targets the root of the problem which is the weakened cornea in keratoconus. CXL significantly increases the number of collagen bonds between the corneal layers, in effect returning the cornea to a more stable state. In contrast to other procedures like intrastromal ring segments or laser surgery, only CXL offers the potential to target the underlying cause and thus offer long-term benefits for those suffer from keratoconus.. More interestingly, CXL can be a part of a process in the management of keratoconus patients. It can be combined with Intacs before or after the procedure, and fine tuned with surface laser procedures. ...
PURPOSE: To identify risk factors for opaque bubble layer (OBL) formation and compare the incidence of OBL using a cone modification technique versus the original technique for LASIK flap creation using the VisuMax laser (Carl Zeiss Meditec, Jena, Germany). METHODS: This retrospective study examined videos of flap creation using the VisuMax laser to identify OBL occurrence. Eyes were divided into three groups: eyes where OBL occurred using the original technique (OBL group), eyes where OBL did not occur using the original technique (no OBL group), and eyes in which the cone modification technique was used for LASIK flap creation (larger flap diameter) (cone modification technique group ...
CURRENT RESEARCH GRANTS 1. RO1 Grant from National Institute of Health (NEI). Ocular Mucosal Immunity Induced By HSV1 Lipopeptides. EY14900. September, 30, 2003 to May 31 2012. Principal Investigator: Lbachir BenMohamed. 2. Discovery Eye Foundation. Therapeutic vaccine against ocular herpes in HLA transgenic rabbits.. January, 1, 2009 to December 31 2015. Principal Investigator: Lbachir BenMohamed. 3. RO1 Grant from National Institute of Health (NEI). Role of TGFbeta in Corneal Stromal Wound Healing. EY007348 March, 1st, 2006 to April 31 2011. Co-Investigator (10%): Lbachir BenMohamed. 4. RO1 Grant from National Institute of Health (NEI). Corneal HSV-1: Immunopathologic Mechanisms of HSK. EY018171. January, 1st, 2008 to December 31 2010. Co-Investigator (10%): Lbachir BenMohamed. 5. R24 Core grant from National Institute of Health (NEI). Vision Research Infrastructure Development. EY016663. June 1, 2005-May 31, 2010. Co-Investigator, Director of Cell Culture Module (5%): Lbachir BenMohamed. 6. ...
Introduction and Background. The cornea remains transparent for many reasons. But the reason it is important to understand what is happening in a blue eye is that in a normal cornea, the collagen fibrils that make up the corneal stroma are uniformly spaced in a configuration that allows visible light to go through. While other tissues in the body have higher water contents, the cornea is only 78% water. This allows the collagen fibrils to maintain their optimal spacing. When excess water enters the cornea, it increases the space between the corneal fibrils, causing a wavelength-dependent decrease in light transmission through the cornea. This wavelength-dependent decrease is why the cornea not only turns cloudy when it is edematous, but it acquires a blueish hue as well.. As the cornea is surrounded by water (the tear film in front and the aqueous humor behind), the cornea must work very hard to regulate the water levels within its stroma. The outermost cells of the corneal epithelium have ...
A team of outstanding surgeons from all over the world who have made recent major advances in the management and treatment of Keratoconus, have shared their invaluable experiences in this book. Each one of them is a brilliant medical writer as well; the quality which is not commonly found. Includes latest techniques which were expressly ideated to strengthen or to replace a weakened corneal stroma. Collagen cross-linking may prove to be very effective in the management of Ectasia. ...
The mice were adminstered doxycycline (2 mg/mL) for seven days in the drinking water with 5% sucrose or with 9-t-butyl doxycycline at (02 and 2 mg/mL). When this LH surge reaches a certain point, bentyl price one of your ovaries releases an egg? Por lo tanto, interim himcolin gel price in india hindi Augmentin Bid jarabe 457 mg/5 ml posee las propiedades distintivas de un antibiótico de amplio espectro y un inhibidor de la beta-lactamasa. โรคซึมเศร้า เริ่มต้นด้วยขนาด 25-50 มกต่อวัน แล้วค่อย ๆ ปรับเป็นวันละ 150 มก. I would stop using Retin A for two days before the facial and for two days after. So my dermatologist perscribed retin a to help with sun damage and the lines! The results of this study were consistent with previous HEDS data showing that patients with a history of ocular HSV not affecting the corneal stroma rarely experience episodes of HSV stromal keratitis later? ...
RESULTS: The receptors for LXA4, ALX/Fpr-rs-2 and for RvE1, ChemR23 were each expressed by epithelium, stromal keratocytes, and infiltrated CD11b(+) cells in corneas. Compared to the vehicle-treated eye, ATLa-, RvD1-, and RvE1-treated eyes had reduced numbers of infiltrating neutrophils and macrophages and reduced mRNA expression levels of TNF-alpha, IL-1 alpha, IL-1 beta, VEGF-A, VEGF-C, and VEGFR2. Animals treated with these mediators had significantly suppressed suture-induced or IL-1 beta-induced hemangiogenesis (HA) but not lymphangiogenesis. Interestingly, only the application of ATLa significantly suppressed VEGF-A-induced HA ...
The analysis of the stress-strain curves showed a different corneal response between treated (TS) and untreated (CS) samples. TS showed a stiffer behaviour than CS. The treated corneal tissue (TS) resulted 1.8, 1.6, 1.7 and 1.5 fold stiffer than the untreated sample (CS) at 6, 8, 10 and 12% stretch, respectively. The histological analysis of TS showed an increased fibrillar density with decrease of interlamellar space in comparison with CS. Keratocyte apoptosis was observed in TS even at deep stromal level. There is an evident absence of nuclei in the treated sample compared to the control. Interlamellar spaces correspond to the presence of a cellular body, but the nucleus could not be present at the depth of the tissue section ...
Spontaneous symmetry breaking and motility initiation of keratocytes. Phase-contrast time-lapse video of a keratocyte spontaneously breaking symmetry ...
Keratoconus treatment for age above 45 years includes intacs, PIE, toric lens and c3R. Expert keratoconus surgeon can decide best option for your lifestyle.
Corneal inlays to correct refractive errors are not new-various materials have been tried for more than 50 years to correct blurred vision. The greatest barriers to success of corneal inlays have been a lack of biocompatibility with the cornea, the difficulty of placing them within the corneal stroma safely, and refractive predictably. ...
Birk, D E et al Collagen type I and type V are present in the same fibril in the avian corneal stroma.. The Journal of Cell Biology 106.3 (1988): 999-1008. Web. 16 Jan. 2018. ...
Very impressive attention to texture, lighting, and details. What software did you use to create these? It looks amazing! 7 years ago ...
3D printing is bringing ideas and images into the third dimension; being used in engineering and scientists have used the technology to print the first corneas.
second wave of migratory cells containing presumptive keratocytes invades the matrix, leading to formation of inner cornea and outer cornea. A unique cell mass (stroma attracting center) connects the two layers like the center pole of a tent ...
The cornea is the windshield through which you see the world. It is composed of collagen and different layers of transparent cells on the outermost part of the eye. Sometimes things can happen to the cornea, it can become badly scarred, thinned out or cloudy due to disorders of the cornea or due to injury. This can dramatically reduce your…
GPI 2780 Cornea Eye Cross-Section Model is a normal oversized eye model includes 4 interchangeable corneas that show various cornea conditions.
Cornea, the transparent layer of eye refracts light onto the lens. Dr. Agarwals Cornea Foundation offers comprehensive treatment for all cornea diseases.
Cornea surgery has developed rapidly over the last few years, with techniques that were unthinkable just a few years ago becoming increasingly commonplace. Examine the latest techniques in the field together with the Asia Cornea Society.. ...
Televiziunea Cornea TV emite deocamdata doar pe internet, solicitarea pentru licența depusa in aprilie 2018 la CNA fiind respinsa. Fanii emisiunii La Hanu lu Nea Mărin, difuzata pana in 2018 de Inedit TV, vor putea urmari show-ul preferat numai aici, la Cornea TV ...
As light enters the transparent cornea, the cornea bends the light rays. This is the first step in the focusing process, which projects a coherent im...
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MOLA HIDATIDOSA Ibu Reni A.Defenisi • Mola hidatidosa ialah kehamilan abnormal, dengan ciri-ciri stroma vilus korialis langka vaskularisasi, dan ...
The corneal stroma becomes severely disorganised; the lamellar arrangement of the fibrils becomes disrupted. Management of CHED ... Congenital hereditary corneal dystrophy (CHED) is a form of corneal endothelial dystrophy that presents at birth. CHED was ... Posterior polymorphous corneal dystrophy (for the condition previously referred to as CHED1) Corneal dystrophy Bowes Hamill, M ... The age that corneal transplantation is required is variable, however, it is usually necessary fairly early in life. ...
Corneal epithelium suffered abrasions and stroma were punctured. There was also an infestation during the 1997-1998 summer and ...
Shimazaki, J; Harashima, A; Tanaka, Y (9 October 2009). "Corneal endotheliitis with cytomegalovirus infection of corneal stroma ... "Mapping owl's eye cells of patients with cytomegalovirus corneal endotheliitis using in vivo laser confocal microscopy". ...
doi:10.1111/j.1399-3054.2006.00737.x. Sayers, Zehra (1982). "Synchrotron X-ray diffraction study of corneal stroma". Journal of ... Sayers, Zehra (1981). "Interpretation of the meridional X-ray diffraction pattern from collagen fibrils in corneal stroma". ...
... corneal stroma or the dental pulp of deciduous (baby) teeth. The cells do not have the capacity to reconstitute an entire organ ... "Mesenchymal stem cells in the human corneal limbal stroma". Investigative Ophthalmology & Visual Science. 53 (9): 5109-16. doi: ... "Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using ...
Neutrophils infiltrate the corneal stroma in a diffuse, multifocal pattern. Infiltration is confined to the surgical flap ... DLK can also occur following myopic keratomileusis, in which a disc of corneal tissue is removed, shaped and sutured back into ...
Quantock, A.J. and R.D. Young, Development of the corneal stroma, and the collagen-proteoglycan associations that help define ... Hamrah, P., et al., The corneal stroma is endowed with a significant number of resident dendritic cells. Invest Ophthalmol Vis ... Keratocytes are flattened cells found dispersed within the corneal stroma. The primary role of this sparse population of cells ... Corneal nerves serve as a form of defense by detecting the presence of foreign bodies on the corneal surface. This leads to ...
These occur because descemet's membrane is less elastic than the corneal stroma. Tears are usually peripheral, concentric with ...
"The corneal stroma is endowed with a significant number of resident dendritic cells." Investigative Ophthalmology & Visual ... Hamrah P, Liu Y, Zhang Q, Dana MR (February 2003). "The corneal stroma is endowed with a significant number of resident ... "Corneal immunity is mediated by heterogeneous population of antigen‐presenting cells." Journal of Leukocyte Biology 74, no. 2 ( ... "Novel characterization of MHC class II-negative population of resident corneal Langerhans cell-type dendritic cells." ...
The extracellular mesenchyme forms the sclera, the corneal endothelium and stroma, blood vessels, muscles, and vitreous. The ... Surface ectoderm produces the following parts: lens corneal epithelium skin of eyelid Neural crest cells are themselves derived ... Surface ectoderm forms the lens, corneal epithelium and eyelid. ... Bowman's membrane and stroma According to Liem et al., the ...
When this happens, the blade inadvertently cuts into the collagenous corneal stroma and creates an irregularity. If this ... Less damage to corneal nerves, hence safer in dry eyes If cornea is abnormal for LASIK, epilasik may still be an option ... Chen WL.Bilateral complicated stromal dissections during mechanical epikeratome separation of the corneal epithelium.J Refract ... of cases is corneal stromal incursion during the microkeratome pass. ...
Their further investigation of this pre-Descemet stroma confirms that it is stroma, and not a new corneal layer...[D]espite ... Their further investigation of this pre-Descemet stroma confirms that it is stroma, and not a new corneal layer. "Scientists ... The images showed a thin layer of corneal collagen between the corneal stroma and Descemet's membrane. The findings were ... During surgery, tiny air bubbles are injected into the corneal stroma in what is known as the "big bubble technique". Sometimes ...
The second step of the procedure uses an excimer laser (193 nm) to remodel the corneal stroma. The laser vaporizes the tissue ... Performing the laser ablation in the deeper corneal stroma provides for more rapid visual recovery and less pain than the ... Both the depth of the corneal incisions and the degree of central corneal flattening correlated with the laser energy applied. ... Those with Fuchs' corneal endothelial dystrophy, corneal epithelial basement membrane dystrophy, retinal tears, autoimmune ...
... slowly progressive thinning of the peripheral corneal stroma. The cause of Terrien marginal degeneration is unknown, its ...
The corneal stroma consists of approximately 200 layers of mainly type I collagen fibrils. Each layer is 1.5-2.5 μm. Up to 90% ... Corneal pachymetry Corneal reflex Corneal tattooing Corneal topography Eye disease Keratometry List of keratins expressed in ... If the corneal stroma develops visually significant opacity, irregularity, or edema, a cornea of a deceased donor can be ... Corneal stroma (also substantia propria): a thick, transparent middle layer, consisting of regularly arranged collagen fibers ...
These cells lead to formation of most of the anterior segment structures of the eye (corneal stroma & endothelium, iris stroma ... refers to a spectrum of diseases characterized by malformations of the irido-corneal angle of the anterior chamber of the eye. ...
In the corneal stroma, keratocytes within the wounded area undergo apoptosis, leaving the stroma devoid of cells that must be ... For example, when corneal integrity is compromised, epithelial cells quickly cover the damaged area by proliferation and ... "Role of Thrombospondin-1 in Repair of Penetrating Corneal Wounds". Investigative Ophthalmology & Visual Science. 54 (9): 6262- ...
"Arc-scanning very high-frequency digital ultrasound for 3D pachymetric mapping of the corneal epithelium and stroma in laser in ... "Arc-scanning very high-frequency digital ultrasound for 3D pachymetric mapping of the corneal epithelium and stroma in laser in ... Vanderbeek, Brian L; Silverman, Ronald H; Starr, Christopher E (2009). "Bilateral Salzmann-like nodular corneal degeneration ... "Central corneal thickness measured by the Orbscan II system, contact ultrasound pachymetry, and the Artemis 2 system". Journal ...
As the inflammation is reduced, the stroma appears to be thin and the corneal topography changes to show an area of depression ... There is no iritis, corneal stroma or breech of Bowman's membrane. The ulceration can be accompanied by conjunctivitis, without ... The corneal response to the treatment appears to be as expected until the four-day mark where the corneal inflammation and ... It could also show corneal edema which leads to thickening the corneal to 30%. Extensive conjunctival injection, which is an ...
The flap is lifted like a hinged door, targeted tissue is removed from the corneal stroma, again with the microkeratome, and ... Customized Transepithelial No-touch (C-TEN) is an innovative strategy for corneal surgery that avoids any corneal manipulation ... is a procedure that also changes the shape of the cornea using an excimer laser to ablate the tissue from the corneal stroma, ... A femtosecond laser cuts a lenticule within the corneal stroma. Afterwards, a LASIK-like flap is cut which can be lifted to ...
... the most common injuries seen in this zone are foreign debris retention by the conjunctiva or corneal epithelium/stroma, as ... Application of the Seidel Test in Zone I will rule out seepage of the anterior chamber through a corneal perforation, while ... The Seidel test may be used to evaluate the status of the anterior chamber, thereby determining the presence of corneal ...
For candidates to qualify for this treatment, they have their corneal stroma thickness checked to make sure that post operative ... in that it uses a single femtosecond laser referenced to the corneal surface to cleave a thin lenticule from the corneal stroma ... Liu R, Zhao J, Xu Y, Li M, Niu L, Liu H, Sun L, Chu R, Zhou X (2015). "Femtosecond Laser-Assisted Corneal Small Incision ... There are limited studies on corneal wound healing and inflammatory response after this treatment has been carried out. There ...
The second was using Nomarski optics to study how corneal fibroblasts migrated through the stroma in vivo and in collagen gels ... They cultured corneal epithelium in all types of ECM and were able to show that the ECM can induce cell differentiation and ... They used avian corneal epithelium rather than salamander epidermis in order to provide more examples of epithelium secreting ... The first was on corneal endothelium morphogenesis and they, with Sam Meller, showed that the key constraint on migration was ...
Nodule formation can also occur when the abnormal corneal endothelium causes contractions around the iris stroma [1]. ... of the corneal endothelium, corneal edema, increased intraocular pressure, peripheral anterior synechiae, and iris changes [1,2 ... Facts About the Cornea and Corneal Disease The National Eye Institute (NEI).. ... of diseases characterized by slowly progressive abnormalities of the corneal endothelium and features including corneal edema, ...
The brush contains a small burr that is capable of penetrating an eye's corneal stroma to reach small foreign objects lodged in ...
As the disease progresses and infiltrates the corneal stroma, a classic "ring infiltrate" may be present on examination ( ... In cases of corneal ulceration or perforation, or if corneal scarring is severe, corneal transplant may be required. This ... while also preventing deep penetration of cysts into the corneal stroma. Cysts that are not eradicated from the cornea will ... Soft contact lenses are more adherent to the corneal surface than hard lenses, which allows the Acanthamoeba organism to bind ...
Corneal epithelial infiltrates Keratitis Corneal ulcer Corneal stroma Corneal neovascularisation Corneal oedema Corneal ... Ptosis Giant papillary conjunctivitis Superior limbic keratoconjunctivitis Epithelium Corneal abrasion Corneal erosion Contact ... infiltrates Corneal endothelium Endothelial polymegathism Effects of long-term contact lens wear on the cornea. ...
... a small amount of tissue from the corneal stroma at the front of the eye, just under the corneal epithelium. The outer layer of ... With PRK, the corneal epithelium is removed and discarded, allowing the cells to regenerate after the surgery. The procedure is ... The amount of corneal hazing after surgery is also decreased with brush technique. The platelet activating factor LAU-0901 has ... Uncomplicated, successful corneal refractive surgery does not require a waiver and is noted as information only. The Navy and ...
When used in corneal surgery, picosecond and nanosecond disruptors are used on the lamellae of the corneal stroma, and the ... This modifies the outer corneal curvature, which affects the refractive property of the eye. Fankhauser, Franz; Kwasniewska, ...
Anterior corneal epithelium, Anterior limiting lamina (Bowman's layer), Substantia propria (stroma comprising the majority of ... corneal thickness),Posterior limiting lamina (Descemet's membrane) Posterior epithelium (endothelium). The iris in eagles ...
These infectious agents produce proteases and collagenases which break down the corneal stroma. Complete loss of the stroma can ... Refractory corneal ulcersEdit. Main article: Recurrent corneal erosion. Refractory corneal ulcers are superficial ulcers that ... which is taken up by exposed corneal stroma and appears green, helps in defining the margins of the corneal ulcer, and can ... Melting ulcers are a type of corneal ulcer involving progressive loss of stroma in a dissolving fashion. This is most commonly ...
The hallmark of Schnyder corneal dystrophy is the accumulation of crystals within the corneal stroma which cause corneal ... Corneal stromal dystrophies - Macular corneal dystrophy is manifested by a progressive dense cloudiness of the entire corneal ... In the past, the designation vortex corneal dystrophy (corneal verticillata) was applied to a corneal disorder characterized by ... Posterior corneal dystrophies - Fuchs corneal dystrophy presents during the fifth or sixth decade of life. The characteristic ...
It is used for localizing and identifying the presence of filamentary fungal elements in the corneal stroma in cases of ...
In the late 1970s, intraocular lens implantation was often followed by severe corneal edema, due to endothelial cell damage ... "Prostate cancer increases hyaluronan in surrounding nonmalignant stroma, and this response is associated with tumor growth and ... corneal transplantation, cataract surgery, glaucoma surgery, and surgery to repair retinal detachment). Other biomedical ...
Neurotrophic: corneal nerve damage causes persistent epithelial defect, thinning and even perforation. Cornea becomes ... Mucous plaques: linear grey elevations loosely adherent to the underlying diseased epithelium/stroma. Chronic. Onset between 3 ... Disciform Keratitis (Disciform Endotheliitis): a disc of corneal oedema, folds in Descemet's membrane, mild inflammation ... Uncommon Necrotising interstitial keratitis: Characterised by stromal infiltrates, corneal thinning and possibly perforation. ...
Terletak di antara epitelium dan stroma di kornea. Lapisan ini terdiri dari serat kolagen kuat dan membantu kornea ... "A comparative study of Bowman's layer in some mammals: Relationships with other constituent corneal structures." European ...
Mar-Apr 1995) "Refractive index of the human corneal epithelium and stroma". J Refract Surg. 11 (2): 100-105. ...
During development of the embryo, the corneal stroma is derived from the neural crest (a source of mesenchyme in the head and ... At its centre, human corneal stroma is composed of about 200 flattened lamellæ (layers of collagen fibrils), superimposed one ... "Mesenchymal stem cells in the human corneal limbal stroma". Invest Ophthalmol Vis Sci. 53 (9): 5109-16. doi:10.1167/iovs.11- ... Macular corneal dystrophy, associated with the loss of keratan sulfate. References[edit]. *^ Oyster, CW (1999). "8". The human ...
... and inserts two crescent or semi-circular shaped ring segments between the layers of the corneal stroma, one on each side of ... Corneal collagen cross-linking[edit]. Corneal collagen cross-linking may delay or eliminate the need for corneal ... Corneal transplantation, also known as corneal grafting, is a surgical procedure where a damaged or diseased cornea is replaced ... Intrastromal corneal ring segments[edit]. In corneal disorders where vision correction is not possible by using contact lenses ...
March-April 1995). "Refractive index of the human corneal epithelium and stroma". J Refract Surg. 11 (2): 100-105. PMID 7634138 ...
Daxer A, Misof K, Grabner B, Ettl A, Fratzl P. "Collagen fibrils in the human corneal stroma: structure and aging." Invest ... Daxer A, Fratzl P. "Collagen fibril orientation in the human corneal stroma and its implication in keratoconus." Invest ... "Structural transformation of collagen fibrils in corneal stroma during drying. An x-ray scattering study." Biophys J. 1993 Apr; ... Facts About The Cornea and Corneal Disease National Eye Institute (NEI). *Keratoconus (Irregular Astigmatism), Patient Support ...
... throughout the middle and anterior stroma. During an eye examination, the doctor sees these deposits in the stroma as clear, ... Corneal abrasions are also a common and recurrent feature in people who suffer specific types of corneal dystrophy, such as ... Fowler, Grant C. (2011), "Corneal Abrasions and Removal of Corneal or Conjunctival Foreign Bodies", Pfenninger and Fowler's ... Ahmed, Faheem; House, Robert James; Feldman, Brad Hal (1 September 2015). "Corneal Abrasions and Corneal Foreign Bodies". ...
corneal stroma. *Descemet' membraan. *corneal endothelium.. 2012. aastal avastasid Inglise Nottinghami ülikooli teadlased, et ... Harminder S. Dua, Lana A. Faraj, Dalia G. Said, Trevor Gray, James Lowe, Human Corneal Anatomy Redefined: A Novel Pre- ... W M Bourne, Biology of the corneal endothelium in health and disease, Eye (2003) 17, 912-918. doi:10.1038/sj.eye.6700559, ...
Daxer, A; Misof, K; Grabner, B; Ettl, A; Fratzl, P (1998). "Collagen fibrils in the human corneal stroma: Structure and aging ... Daxer, A; Fratzl, P (1997). "Collagen fibril orientation in the human corneal stroma and its implication in keratoconus". ... "Structural transformation of collagen fibrils in corneal stroma during drying. An x-ray scattering study". Biophysical Journal ...
These infectious agents produce proteases and collagenases which break down the corneal stroma. Complete loss of the stroma can ... The epithelium sticks to the basement membrane, which also separates the epithelium from the stroma. The corneal stroma ... Posterior to the stroma is Descemet's membrane, which is a basement membrane for the corneal endothelium. The endothelium is a ... Superficial and deep corneal ulcers[edit]. Corneal ulcers are one of the most common eye diseases in dogs. They are caused by ...
... loss of hydration within the corneal stroma is believed to be the cause of corneal haze, thus supporting the long-held ... and affect the motility of corneal endothelial cells.[17] In summary, KS plays an anti-adhesive role, which suggests very ... In disease states such as macular corneal dystrophy, in which GAGs levels such as KS are altered, ... hypothesis that corneal transparency is a dependent on proper levels of keratan sulfate. Keratan sulfate GAGs are found in many ...
corneal epitheliuma. Bowman's membranea. corneal stroma. Descemet's membranea. corneal endotheliuma. Artikulazioa. Esklerotika ...
There may be an increase in the number of mucopolysaccharides in the corneal stroma. The Bowman's layer of the cornea may be ... Corneal collagen cross-linking[edit]. Main article: Corneal collagen cross-linking. There is evidence suggesting corneal ... The gold standard diagnostic test for PMD is corneal topography.[2] However, it may not as specific as corneal pachymetry, ... PMD lacks apical corneal scarring, Rizutti's phenomenon, Munson's sign, and the central corneal thickness is usually normal.[2] ...
... is a rare disorder obtained during development characterized by adhesions due to malformations of the posterior corneal stroma ... the absence of Descemet's membrane and the corneal endothelium, and corneal opacities. This syndrome can be attributed to fetal ...
The corneal endothelial cells normally do not undergo mitotic cell division, and cell loss results in permanent loss of ... When endothelial cell counts drop too low, the pump starts failing to function and fluid moves anterior into the stroma and ... "Descemet's membrane endothelial keratoplasty versus Descemet's stripping automated endothelial keratoplasty for corneal ...
... stretch reflex striae gravidarum stria medullaris thalami stria terminalis striate cortex striate nucleus striatum stroma ... contralateral conus elasticus conus medullaris Coracobrachialis muscle coracoid coracoid process cordotomy cornea corneal ...
Pericytes present in the stroma of tumors of breast cancer patients express MMP-9. Animal models deficient of MMP-9 display ... Kato, T; Kure, T; Chang, JH; Gabison, EE; Itoh, T; Itohara, S; Azar, DT (2001). "Diminished corneal angiogenesis in gelatinase ... Both enzymes inhibit bFGF induced vascularization in the corneal pocket assay and inhibit VEGF induced angiogenesis in the ... For example, MMP-2 knockout mice develop normally but display significant inhibition of corneal angiogenesis. Numerous ...
The condition can cause oedema of the surrounding tissue, including the stroma, and can cause irritation and pain. If it is ... "Mumps-Induced Corneal Endotheliitis" 2004 Cornea Chee, Soon-Phaik; Bacsal, Kristine; Jap, Aliza; Se-Thoe, Su-Yun; Cheng, Ching ... "Experimental Corneal Endotheliitis in Rabbits" 2000 Association for Research in Vision and Ophthalmology Singh, Kirti " ... Li; Tan, Ban Hock (2007). "Corneal Endotheliitis Associated with Evidence of Cytomegalovirus Infection". Ophthalmology. 114 (4 ...
High and low angle X-ray diffraction patterns from the corneal stroma give information about the mean intermolecular spacing of ... Ageing of the human corneal stroma: structural and biochemical changes Biochim Biophys Acta. 1992 Mar 20;1138(3):222-8. doi: ... High and low angle X-ray diffraction patterns from the corneal stroma give information about the mean intermolecular spacing of ... Between birth and 90 years there is an increase in the cross-sectional area associated with each molecule in corneal collagen ...
Home Clinical Education Cornea Cellular Therapy of the Corneal Stroma for Advanced Keratoconus ... Cataract Surgery in Patients with Corneal Pathology Brandon D. Ayres, MD, Marjan Farid, MD, Elizabeth Yeu, MD, Clara Chan, MD 1 ... Depth-Dependent Analysis of Corneal Cross-Linking Performed over or Under the Flap By Brillouin Microscopy ...
Defects in the corneal stroma caused by trauma or diseases such as macular corneal dystrophy and keratoconus can be detrimental ... Bioactive self-assembled peptide nanofibers for corneal stroma regeneration. By G. Uzunalli, Z. Soran, T.S. Erkal, Y.S. Dagdas ... Home / KnowledgeBase Articles / Bioactive self-assembled peptide nanofibers for corneal stroma regeneration ... Human corneal keratocyte cells cultured on laminin-mimetic peptide nanofibers retained their characteristic morphology, and ...
Development of the corneal stroma, and the collagen-proteoglycan associations that help define its structure and function. ... Bin Kong, Shengli Mi, Electrospun Scaffolds for Corneal Tissue Engineering: A Review, Materials, 2016, 9, 8, 614. CrossRef ... Shikha Chawla, Sourabh Ghosh, Establishment of in vitro model of corneal scar pathophysiology, Journal of Cellular Physiology, ... Sunil K. Parapuram, William Hodge, The integrin needle in the stromal haystack: emerging role in corneal physiology and ...
Surface ablation of corneal stroma with few-cycle laser pulses at 800 nm L. Hoffart, P. Lassonde, F. Légaré, F. Vidal, N. ... Wavelength dependence of femtosecond laser ablation threshold of corneal stroma G. Olivié, D. Giguère, F. Vidal, T. Ozaki, J.-C ... D. W. Leonard and K.M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," ... D. W. Leonard and K.M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," ...
Exogenous TGFβ3 promotes mouse corneal stroma development Lingling Zhang; Lung-Kun Yeh; Yujin Zhang; Yuka Okada; Yen-Chiao Wang ... Exogenous TGFβ3 promotes mouse corneal stroma development You will receive an email whenever this article is corrected, updated ... Methods : Primary corneal stroma fibroblasts were treated with BMP4, TGFβ3 or PBS (control) and related gene expressions were ... TEM revealed that TGFβ3 promotes mouse corneal stroma maturation by increasing stromal cell density and cell activates (more ER ...
Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal ... Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. ... corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal ... 3D bioprinting of a corneal stroma equivalent Exp Eye Res. 2018 Aug;173:188-193. doi: 10.1016/j.exer.2018.05.010. Epub 2018 May ...
Collagen type I and type V are present in the same fibril in the avian corneal stroma.. D E Birk, J M Fitch, J P Babiarz, T F ... Collagen type I and type V are present in the same fibril in the avian corneal stroma. ...
Its primary structural and mechanical element is the corneal stroma, comprising over 90% of corneal thickness. The stroma ... Meek KM Boote C. The organization of collagen in the corneal stroma. Exp Eye Res . 2004; 78: 503-512. [CrossRef] [PubMed] ... A large body of research has been devoted to exploring the structure and biomechanics of the corneal stroma. 1,4-11 However, ... Development of the corneal stroma, and the collagen-proteoglycan associations that help define its structure and function. Dev ...
... images of the human corneal stroma is presented. The corneal OCT data is first processed using a state-of-the-art despeckling ... Cell density distribution analysis was carried in 3D UHR-OCT images of the human corneal stroma, acquired in-vivo. The cell ... Automated detection and cell density assessment of keratocytes in the human corneal stroma from ultrahigh resolution optical ... Automated detection and cell density assessment of keratocytes in the human corneal stroma from ultrahigh resolution optical ...
The Kinetics of Chemically Induced Nonequilibrium Swelling of Articular Cartilage and Corneal Stroma S. R. Eisenberg and A. J. ... The Kinetics of Chemically Induced Nonequilibrium Swelling of Articular Cartilage and Corneal Stroma. ASME. J Biomech Eng. 1987 ... Return to: The Kinetics of Chemically Induced Nonequilibrium Swelling of Articular Cartilage and Corneal Stroma ... To Understand Myopic Alleviation by Conducting Finite Element Structural Analysis of a Cornea With an Intrastromal Corneal Ring ...
The primary objective of the current study was to evaluate the efficacy of acellular porcine corneal stroma (APCS) ... Corneal stroma substitutes. The APCS graft used in the current study was prepared by AiNear Corneal Engineering Corporation ( ... Lamellar keratoplasty treatment of fungal corneal ulcers with acellular porcine corneal stroma. Am J Transplant. 2015;15:1068- ... Zheng J, Huang X, Zhang Y, Wang Y, Qin Q, Lin L, Jin X, Lam C, Zhang J. Short-term results of acellular porcine corneal stroma ...
Dry Eye Zone: Corneal Stroma ► eMedicine: Descemet Membrane Folds Medical Textbooks for Health Professionals and Consumers ... National Eye Institute /US/: Facts About The Cornea and Corneal Disease / The Cornea and Corneal Disease ... Corneal Stroma. 2 Anatomy - Sense Organs / Eyes / 2 Anterior Eye Segment / 2 Cornea / ► Corneal Stroma ...
Hanlon, S. D., Behzad, A. R., Sakai, L. Y., & Burns, A. R. (2015). Corneal stroma microfibrils. Experimental Eye Research, 132 ... Fingerprint Dive into the research topics of Corneal stroma microfibrils. Together they form a unique fingerprint. * ... Corneal stroma microfibrils. / Hanlon, Samuel D.; Behzad, Ali R.; Sakai, Lynn Y.; Burns, Alan R. ... Hanlon, Samuel D. ; Behzad, Ali R. ; Sakai, Lynn Y. ; Burns, Alan R. / Corneal stroma microfibrils. In: Experimental Eye ...
The corneal stroma becomes severely disorganised; the lamellar arrangement of the fibrils becomes disrupted. Management of CHED ... Congenital hereditary corneal dystrophy (CHED) is a form of corneal endothelial dystrophy that presents at birth. CHED was ... Posterior polymorphous corneal dystrophy (for the condition previously referred to as CHED1) Corneal dystrophy Bowes Hamill, M ... The age that corneal transplantation is required is variable, however, it is usually necessary fairly early in life. ...
Szentmáry, N., Nagy, Z. Z., Resch, M., Szende, B., & Süveges, I. (2005). Proliferation and apoptosis in the corneal stroma in ... Szentmáry, N, Nagy, ZZ, Resch, M, Szende, B & Süveges, I 2005, Proliferation and apoptosis in the corneal stroma in longterm ... Proliferation and apoptosis in the corneal stroma in longterm follow-up after photorefractive keratectomy. Pathology Research ... Proliferation and apoptosis in the corneal stroma in longterm follow-up after photorefractive keratectomy. In: Pathology ...
Corneal epithelium suffered abrasions and stroma were punctured. There was also an infestation during the 1997-1998 summer and ...
These corneal qualities have also allowed for high-resolution in vivo imaging of non-ocular tissue transplanted into the ... Confocal Imaging of Myeloid Cells in the Corneal Stroma of Live Mice Edited by. Jia Li ... How to cite: Chan, M. F. and Werb, Z. (2015). Confocal Imaging of Myeloid Cells in the Corneal Stroma of Live Mice. Bio- ... Chan, M. F. and Werb, Z. (2015). Confocal Imaging of Myeloid Cells in the Corneal Stroma of Live Mice. Bio-protocol 5(13): ...
Stem cells (Dayton, Ohio). Keratocytes of the corneal stroma secrete a specialized extracellular matrix essential for vision. ... Keratocytes of the corneal stroma secrete a specialized extracellular matrix essential for vision. These quiescent cells ... These results demonstrate the presence of a population of cells in the human corneal stroma expressing stem cell markers and ... These cells may be a resource for bioengineering of corneal stroma and for cell-based therapeutics. ...
Cellular delivery into the corneal stroma has been experimentally assayed in vivo in multiple ways: systemic versus local ... improve corneal transparency in metabolic diseases by enhancing the catabolism of the accumulated molecules, generate new ... Cellular therapy of the corneal stroma, with either ocular or extraocular stem cells, has been gaining a lot of interest over ... from different research groups are showing its potential benefits in relation to its capacity to improve or alleviate corneal ...
Tissue engineering of the cornea: orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma ... orthogonal scaffold of magnetically aligned collagen lamellae for corneal stroma reconstruction ...
Age, refractive index of corneal stroma help improve LASIK predicted outcomes. by Healio ophthalmology • 2013/03/26. • 0 ... Factoring patient age and refractive index of the corneal stroma into the treatment profile improved the predictability of ...
Corneal stroma is a very complex structure, composed of 200 lamellae of oriented collagen fibers. This highly complex nature of ... In this study, behavior of human corneal keratocytes on collagen films patterned with parallel channels was investigated. ...
Anterior Chamber Associated Immune Deviation ACAID Corneal Immune Privilege Corneal Antiangiogenic Privilege ... pupillary retinal detachment retinopathy retinoschisis risk Schlemms canal Schl tzer-Schrehardt secondary stem cells stroma ... cataract extraction cataract surgery choroidal ciliary body ciliary muscle Clin Exp Ophthalmol clinical conjunctival corneal ...
Purpose: Collagen fibrils in the corneal stroma in macular corneal dystrophy, on average, are more closely spaced than in the ... Structural Collagen Alterations in Macular Corneal Dystrophy Occur Mainly in the Posterior Stroma. Current Eye Research 35 (7 ... Structural Collagen Alterations in Macular Corneal Dystrophy Occur Mainly in the Posterior Stroma ... This study was conducted to investigate if this occurs uniformly across the stroma or is more prevalent at certain stromal ...
Factors Affecting Corneal Strip Stroma-to-Stroma Adhesion Edward P Perez, MD, PhD; Blanca Viramontes, MD; Paulo Schor, MD; ... Corneal Aberrations and Visual Performance After Radial Keratotomy Raymond A Applegate, OD, PhD; Howard C Howland, PhD; Richard ... A Quantitative Corneal Topography Index for Detection of Keratoconus Mohammad H Dastjerdi, MD; Hassan Hashemi, MD ... Refractive versus Corneal Changes After Photorefracive Keratectomy for Astigmatism Noel A Alpins, FRACO, FRCOphth, FACS; ...
V. Corneal Stroma. VI. Sclera. References. Author Index. Subject Index. Details. No. of pages:. 318 Language:. English ...
Penetration of Silicate Nanoparticles into the Corneal Stroma and Intraocular Fluids. Mohammadpour, Mehrdad; Hashemi, Hassan; ... Correlation of Corneal Endothelial Changes With Different Stages of Keratoconus. El-Agha, Mohamed-Sameh H.; El Sayed, Yasmine M ... Pterygium Is Related to a Decrease in Corneal Endothelial Cell Density. Hsu, Min-Yen; Lee, Hsin-Nung; Liang, Chiao-Ying; More ... Corneal Sensitivity in Chronic Inflammatory Demyelinating Polyneuropathy. Bansal, Surbhi; Myneni, Ajay A.; Mu, Lina; More ...
... with inflammation of the underlying corneal stroma (Figure 2A). Figure 2. The development of corneal plaques in Lrig1-deficient ... development of corneal plaques in Lrig1-KO mice was always accompanied by the remodeling of the corneal stroma. The kinetic ... finally leading to the remodeling of the corneal stroma. Only after formation of this microenvironment did the Lrig1-KO corneal ... Wound-induced corneal stem cell replacement impaired by the loss of Lrig1. (A) Percentage of mouse eyes with corneal phenotype ...
... Transparency of the bovine corneal stroma at physiological hydration and its dependence on concentration of the ambient anion. ... The results are related to the known increase of fixed negative charge in the corneal matrix when chloride ions are adsorbed ... It is suggested that the ordering force between corneal collagen fibrils, generated in part by anion binding, may be crucial to ...
  • These results suggest that laminin-mimetic peptide nanofibers provide a promising injectable, synthetic scaffold system for cornea stroma regeneration. (jascoinc.com)
  • The ability to measure precisely their density and spatial distribution in the cornea is important for the understanding of corneal healing processes and the diagnostics of some corneal disorders. (uwaterloo.ca)
  • Purpose: Collagen fibrils in the corneal stroma in macular corneal dystrophy, on average, are more closely spaced than in the normal cornea. (cf.ac.uk)
  • Methods: Microbeam synchrotron X-ray fiber diffraction patterns were obtained in 25 μm steps across the whole thickness of a thin strip of a macular corneal dystrophy cornea obtained at keratoplasty. (cf.ac.uk)
  • We speculate that the ultrastructural abnormalities are more prevalent in the posterior stroma because the structural influence of sulphated keratan sulphate glycosaminoglycans/proteoglycans is high in this region of the cornea. (cf.ac.uk)
  • Infiltration of neutrophils and eosinophils into the mammalian cornea can result in loss of corneal clarity and severe visual impairment. (jimmunol.org)
  • To identify mediators of granulocyte recruitment to the corneal stroma, we determined the relative contribution of chemokine receptors CXC chemokine receptor (CXCR)-2 (IL-8R homologue) and CCR1 using a murine model of ocular onchocerciasis (river blindness) in which neutrophils and eosinophils migrate from peripheral vessels to the central cornea. (jimmunol.org)
  • We found that production of macrophage-inflammatory protein (MIP)-2, KC, and MIP-1α was localized to the corneal stroma, rather than to the epithelium, which was consistent with the location of neutrophils in the cornea. (jimmunol.org)
  • CCR1 deficiency did not inhibit neutrophil or eosinophil infiltration to the cornea or development of corneal opacification. (jimmunol.org)
  • These observations demonstrate the critical role for CXCR2 expression in neutrophil infiltration to the cornea and may indicate a target for immune intervention in neutrophil-mediated corneal inflammation. (jimmunol.org)
  • A corneal ulcer , or ulcerative keratitis , is an inflammatory condition of the cornea involving loss of its outer layer. (wikipedia.org)
  • In veterinary medicine , the term corneal ulcer is a generic name for any condition involving the loss of the outer layer of the cornea, and as such is used to describe conditions with both inflammatory and traumatic causes. (wikipedia.org)
  • The corneal stroma comprises 90 percent of the thickness of the cornea. (wikipedia.org)
  • This has brought about a new hypothesis underpinning epithelial differentiation and migration across the cornea with important implications for our current understanding of stem cell niche biology and corneal (limbal) stem cell therapy - of which my co-workers at the Institute of Genetic Medicine have considerable clinical expertise. (ncl.ac.uk)
  • Corneal esthesiometry is used to measure tactile sensation at the surface of the cornea. (medscape.com)
  • Histological data revealed that the conditional AP-2β NCC knockout (KO) mutants exhibited dysgenesis of multiple structures in the anterior segment of the eye including defects in the corneal endothelium, corneal stroma, ciliary body and disruption in the iridocorneal angle with adherence of the iris to the cornea. (biologists.org)
  • The mutant stroma exhibited impaired keratocyte differentiation with accelerated cell proliferation and reduction in the accumulation of collagen type I. The mutant embryos also showed hyperproliferative nodules in the ocular surface epithelia with anomaly of cornea-type epithelial differentiation and the absence of the epithelial basement membrane. (molvis.org)
  • The cornea must remain transparent to refract light properly and the corneal endothelium ensures the integrity and transparency of the cornea. (hindawi.com)
  • Some retrospective studies have shown Peters anomaly to be the most common congenital corneal opacity presenting to a university cornea clinic. (aao.org)
  • The cornea comprises of three distinct layers: the epithelium, the stroma and the endothelium. (biomedcentral.com)
  • What is the role of GAGs in the stroma of the cornea? (brainscape.com)
  • Corneal Stroma: Swelling of the cornea. (progressivehealth.com)
  • The mechanical properties of the cornea are mainly dependent on its extracellular matrix (stroma), which makes up approximately 90% of its thickness and includes the majority of collagen and proteoglycan content of the tissue. (healio.com)
  • The broad and long-term goal of this Cornea GRC & GRS is to reduce corneal blindness by fostering innovation in corneal research. (grc.org)
  • The principal topic of the GRS is "The Future Directions of Corneal Research", which aims to give the young investigators a notion of the new trends and new directions of cornea/ocular surface tissue research to help them design and plan their future line of research. (grc.org)
  • The mouse cornea comprises an outer epithelium of 5-6 cell layers, a thick stroma and an inner endothelium. (springer.com)
  • The corneal epithelium is derived from a specific group of stem cells, encircling the cornea, that multiply and ultimately form mature corneal cells. (nap.edu)
  • Corneal nutrition is supplied almost exclusively by the aqueous humor circulating behind the cornea in the front of the eye. (nap.edu)
  • The cornea is composed of a multilayered epithelium, Bowman's membrane, stroma, Descemet's membrane, and endothelium ( Figure 1 ). (mdpi.com)
  • Transparency of the cornea is maintained by regulation of stromal hydration through the barrier and pump functions of the corneal endothelium. (mdpi.com)
  • Anterior view of a human cornea and a diagram of the corneal epithelium, stroma and endothelium. (mdpi.com)
  • The advantages of visualizing the different layers of the host and donor cornea, with specific emphasis in the Descemet-stroma interphase, are explained in the written work and captured in the intraoperative images. (intechopen.com)
  • Finally, the innermost corneal endothelium is bathed in the clear aqueous humor that fills the space between the cornea and the iris and pupil. (reasons.org)
  • Corneal epithelium suffered abrasions and stroma were punctured. (wikipedia.org)
  • Epithelial progenitor cells reside in niches at the anterior corneal limbus and ensure a continuous regeneration of the epithelium. (mdpi.com)
  • The number of Langerhans cells was greatly increased in the basal cell layer of the focal corneal epithelium and in Bowman's layer in the four eyes affected by TSPK. (molvis.org)
  • The images revealed highly refractive microdots and refractive bodies immediately below the corneal epithelium, in Bowman's layer, and in the anterior stroma. (molvis.org)
  • Examination of Kynurenine Toxicity on Corneal and Conjunctival Epithelium: In vitro and in vivo Studies. (bioportfolio.com)
  • The metabolic activity of cells was slightly decreased by KYN in the corneal but not in the conjunctival epithelium. (bioportfolio.com)
  • At the tested concentrations, KYN was not toxic for the corneal and the conjunctival epithelium in vitro and did not affect corneal re-epithelization in rabbits in vivo. (bioportfolio.com)
  • To evaluate the effect of diquafosol on corneal epithelium in a dry eye model using Transwell culture and a scopolamine-induced dry eye rat model. (bioportfolio.com)
  • To compare toll-like receptors 2 (TLR2) and 4 (TLR4) expression in cells of corneal and conjunctival epithelium between unilateral KC patients and control subjects. (bioportfolio.com)
  • To investigate the effect of tacrolimus ointment on corneal epithelium. (bioportfolio.com)
  • The study " Autologous cultured corneal epithelium (CECA) for the treatment of unilateral corneal lesions associated with limbal stem cell deficiency" is the first clinical trial of this p. (bioportfolio.com)
  • The epithelium sticks to the basement membrane , which also separates the epithelium from the stroma . (wikipedia.org)
  • Taken together, epithelium-expressed SEMA3C plays a role in corneal epithelial wound closure and sensory nerve regeneration. (diabetesjournals.org)
  • Because the most severe reduction in nerve fiber and branch density occurs in the subbasal nerve plexus close to the corneal epithelium ( 16 ), the role of epithelia in the pathogenesis of DPN and diabetic neurotrophic keratopathy has been suggested ( 5 ), but the molecules involved have not been fully evaluated. (diabetesjournals.org)
  • Corneal epithelium was normal. (hindawi.com)
  • d) Normal corneal epithelium on confocal microscopy. (hindawi.com)
  • a chronic, superficial corneal ulceration in dogs, particularly common in Boxers, that extends into the superficial stroma, often undermining epithelium at the edges. (thefreedictionary.com)
  • In LASIK a flap of corneal epithelium is cut and lifted to allow the underlying stroma to be shaped by the laser. (eurekalert.org)
  • In PRK the corneal epithelium is mechanically removed to allow laser shaping of the stroma. (eurekalert.org)
  • Pax6 fl/fl mice resulted in weak and/or patchy Pax6 immunostaining in the corneal epithelium but caused no corneal abnormalities. (springer.com)
  • Pax6 fl/ + mice with tamoxifen to try to deplete Pax6 in limbal epithelial stem cells (LESCs) which replenish the corneal epithelium. (springer.com)
  • The corneal epithelium is maintained by limbal epithelial stem cells (LESCs), in the ring-shaped limbus, which is a transition zone between the corneal epithelium and the conjunctiva. (springer.com)
  • The adult Pax6 + / − corneal epithelium is thin and fragile, cell turnover is elevated, centripetal movement is disrupted and goblet cells accumulate. (springer.com)
  • Underlying the corneal epithelium is a specialized region of collagen tissue. (nap.edu)
  • Corneal epithelium. (cehjournal.org)
  • The outermost corneal epithelium is made of cells that are constantly produced and shed every week. (reasons.org)
  • It is a multilayered tissue comprising an outer stratified squamous epithelium, an inner monolayered endothelium, and a central stroma with regularly arranged collagen lamellae sparsely populated by keratocytes ( 30 ). (asm.org)
  • The mature corneal epithelium containing five to eight cell layers is present by 6 to 8 weeks after birth ( 15 , 30 ). (asm.org)
  • Functional impairment of the human corneal endothelium can lead to corneal blindness. (mdpi.com)
  • 2017. Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model . (cardiff.ac.uk)
  • Posterior to the stroma is Descemet's membrane , which is a basement membrane for the corneal endothelium . (wikipedia.org)
  • Corneal endothelium could not be evaluated due to intense stromal deposits. (hindawi.com)
  • Descemet stripping automated endothelial keratoplasty (DSAEK) allows selective replacement of the diseased corneal endothelium. (mdpi.com)
  • This review presents current knowledge on the tissue engineering of corneal endothelium using cultured HCECs. (mdpi.com)
  • When primarily damaged, it is reasonable to replace only the corneal endothelium. (mdpi.com)
  • Anterior lamellar transplants have evolved into the deep anterior lamellar keratoplasty (DALK), which is the substitution of the entire stroma while maintaining the recipient's Descemet membrane and endothelium. (intechopen.com)
  • In the present day, the most widely performed techniques are Descemet stripping automated endothelial keratoplasty (DSAEK), in which the recipient's endothelium and a thin layer of posterior stroma are replaced, and Descemet membrane endothelial keratoplasty (DMEK), in which only the Descemet and endothelium are replaced. (intechopen.com)
  • We previously reported that loss of β-catenin in corneal stromal keratocytes triggered corneal precocious maturation, coincident with up-regulation of various growth factors including BMP4 and TGFβ3. (arvojournals.org)
  • These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. (nih.gov)
  • PRK was found to trigger proliferation and apoptosis of corneal keratocytes. (elsevier.com)
  • Keratocytes of the corneal stroma secrete a specialized extracellular matrix essential for vision. (mendeley.com)
  • In this study, behavior of human corneal keratocytes on collagen films patterned with parallel channels was investigated. (metu.edu.tr)
  • This study aimed to investigate the effects of EBN on corneal keratocytes proliferative capacity and phenotypical changes. (biomedcentral.com)
  • Corneal keratocytes from six New Zealand White Rabbits were isolated and cultured until Passage 1. (biomedcentral.com)
  • The proliferative effects of EBN on corneal keratocytes were determined by MTT assay in serum-containing medium (FDS) and serum-free medium (FD). (biomedcentral.com)
  • Both phase contrast micrographs and gene expression analysis confirmed the corneal keratocytes retained their phenotypes with the addition of EBN. (biomedcentral.com)
  • There has been great progress in characterizing the effect of this treatment option on corneal hydrodynamic behavior, collagen fibril diameter, keratocytes, and endothelial cells, among others. (healio.com)
  • The X-ray microscope also allowed the observation of keratocytes and the lamella structure of the scleral stroma. (lancs.ac.uk)
  • Keratocytes are the resident cells of the corneal stroma and their function isto produce extracellular matrix components and to take part in corneal healing after injury, which may occurdue to trauma, infection or surgery. (diva-portal.org)
  • In the studies of this thesis, it is hypothesized that neuropeptides and neurotransmitters areproduced by human keratocytes and that this production is increased in response to corneal injury. (diva-portal.org)
  • The aims of this thesis project were to test these hypotheses and to study the underlying inter- andintracellular mechanisms of the effects of SP and ACh on keratocytes.Results: Cultured primary cells of the human corneal stroma expressed keratocyte markers (keratocan,lumican, CD34, and ALDH), the tachykinins SP and NKA, catecholamines (adrenaline, noradrenaline anddopamine), ACh, and glutamate. (diva-portal.org)
  • Defects in the corneal stroma caused by trauma or diseases such as macular corneal dystrophy and keratoconus can be detrimental for vision. (jascoinc.com)
  • Congenital hereditary corneal dystrophy (CHED) is a form of corneal endothelial dystrophy that presents at birth. (wikipedia.org)
  • However in 2015, the International Classification of Corneal Dystrophies (IC3D) renamed the condition "CHED1" to become posterior polymorphous corneal dystrophy, and renamed the condition "CHED2" to become, simply, CHED. (wikipedia.org)
  • Posterior polymorphous corneal dystrophy (for the condition previously referred to as CHED1) Corneal dystrophy Bowes Hamill, M. (2015). (wikipedia.org)
  • Conclusions: Collagen fibril spacing is reduced and large-diameter collagen fibrils are seen in macular corneal dystrophy type I, with the deep stroma affected more. (cf.ac.uk)
  • These mice exhibit corneal opacity, poor wound healing, are hyporesponsive to LPS-induced sepsis and may be useful in studies of corneal dystrophy, extracellular matrix and neutrophil migration during the immune response. (jax.org)
  • Diseases associated with BMERB1 include Corneal Dystrophy, Lisch Epithelial . (genecards.org)
  • Recurrent corneal erosion (RCE) syndrome can be observed either in the context of a dystrophy of the basement membrane or following corneal trauma. (bioportfolio.com)
  • Other eye conditions can cause corneal ulcers, such as entropion , distichiae , corneal dystrophy , and keratoconjunctivitis sicca (dry eye). (wikipedia.org)
  • Shoch et al described stromal infiltrates in five patients who had RCE and anterior basement membrane dystrophy but with negative corneal cultures in the three cases that were investigated. (bmj.com)
  • Family history and clinical examination are typically sufficient to make the correct diagnosis of lattice corneal dystrophy. (medscape.com)
  • While the genetic basis of lattice corneal dystrophy is known, genetic studies are not commonly used clinically. (medscape.com)
  • A genetic analysis can determine the specific mutation on the TGFBI ( BIGH3 ) gene of chromosome 5, which can allow for the precise diagnosis of the subtype of lattice corneal dystrophy and can be useful for differentiating lattice corneal dystrophy from Avellino dystrophy, granular dystrophy, and Reis-Bückler dystrophy (all which have mutations of the TGFBI ,or BIGH3 , gene). (medscape.com)
  • Lattice corneal dystrophy is an amyloidosis of the corneal stroma. (medscape.com)
  • Because lattice dystrophy is associated with decreased corneal sensation, esthesiometry may be of clinical interest. (medscape.com)
  • Whether decreased corneal sensitivity is associated with increased morbidity in lattice corneal dystrophy is unknown. (medscape.com)
  • A staging system for lattice corneal dystrophy is not commonly used at this time. (medscape.com)
  • Two mutations in the TGFBI (BIGH3) gene associated with lattice corneal dystrophy in an extensively studied family. (medscape.com)
  • Das S, Langenbucher A, Seitz B. Excimer laser phototherapeutic keratectomy for granular and lattice corneal dystrophy: a comparative study. (medscape.com)
  • Morita Y, Chikama T, Yamada N, Morishige N, Sonoda KH, Nishida T. New mode of treatment for lattice corneal dystrophy type I: corneal epithelial debridement and fibronectin eye drops. (medscape.com)
  • Due to technical difficulties, treatment for corneal endothelial decompensation, such as pseudophakic or aphakic bullous keratopathy and Fuchs dystrophy, has been limited to penetrating keratoplasty (PKP). (mdpi.com)
  • We measure the surface ablation threshold fluence of porcine corneal stroma for 100 fs laser pulses, with wavelengths between 800 nm and 1450 nm, generated by a Ti:sapphire-pumped optical parametric amplifier. (osapublishing.org)
  • The primary objective of the current study was to evaluate the efficacy of acellular porcine corneal stroma (APCS) transplantation in various types of infectious keratitis and identify risk factors that may increase APCS graft failure. (biomedcentral.com)
  • Kaplan-Meier survival curve depicting the graft failure episodes in eyes submitted to acellular porcine corneal stroma (APCS) implantation. (biomedcentral.com)
  • Cross-linking full-thickness porcine corneal stroma from the posterior side had no significant stiffening effect. (healio.com)
  • Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. (nih.gov)
  • Although keratoplasty works well in infective keratitis, the shortage of donor corneas is the major limiting factor for the performance of corneal transplantation in many countries, which leads to the development of biomimetic and implantable human corneal substitutes [ 6 ]. (biomedcentral.com)
  • In the last decade, enormous efforts have been put into replicating the corneal stroma in the laboratory to find an alternative to classical corneal transplantation, but this has still not been accomplished due to the extreme difficulty in mimicking the highly complex ultrastructure of the corneal stroma, obtaining substitutes that either do not achieve enough transparency or strength [ 2 , 3 ]. (biomedcentral.com)
  • Management of CHED primarily involves corneal transplantation. (wikipedia.org)
  • The age that corneal transplantation is required is variable, however, it is usually necessary fairly early in life. (wikipedia.org)
  • BACKGROUND/AIMS: Diagnosis of rejection in the mouse model of corneal transplantation is based on subjective judgement of loss of graft transparency. (biomedsearch.com)
  • CONCLUSION: In vivo graft pachymetry provides a new and reliable way to objectively diagnose rejection in the mouse model of corneal transplantation. (biomedsearch.com)
  • Treatments of corneal disorders include medicines, corneal transplantation, and corneal laser surgery. (fpnotebook.com)
  • While many causes of corneal blindness are treatable by donor human corneal transplantation, there is a severe shortage of high quality donor tissues despite innovations such as split grafts [ 2 ] and the use of gamma sterilization techniques to allow for processing of otherwise suboptimal tissue implants. (mdpi.com)
  • These findings indicate that cultured adult HCECs, transplanted with DSAEK surgery, maintain corneal transparency after transplantation and suggest the feasibility of performing DSAEK with HCECs to treat endothelial dysfunction. (mdpi.com)
  • Our goal was to evaluate the effects of KYN on normal human corneal and conjunctival epithelial cells in vitro and the re-epithelization of corneal erosion in rabbits. (bioportfolio.com)
  • Diquafosol Sodium Inhibits Apoptosis and Inflammation of Corneal Epithelial Cells Via Activation of Erk1/2 and RSK: In Vitro and In Vivo Dry Eye Model. (bioportfolio.com)
  • A healing of a corneal ulcer involves two processes: migration of surrounding epithelial cells followed by mitosis (dividing) of the cells, and introduction of blood vessels from the conjunctiva . (wikipedia.org)
  • In vitro studies showed that human corneal epithelial cells and neuronal progenitor cell lines proliferated on these hydrogels. (mdpi.com)
  • The cause is unknown but abnormalities of the basal epithelial cells and anterior stroma have been noted. (thefreedictionary.com)
  • Its primary structural and mechanical element is the corneal stroma, comprising over 90% of corneal thickness. (arvojournals.org)
  • The stroma constitutes more than 90% of the corneal thickness. (biomedcentral.com)
  • The corneal stroma makes up to about 90% of the corneal thickness and is predominantly composed of collagen type I layers. (mdpi.com)
  • Use of ultrasonic pachymetry for measurement of changes in corneal thickness in mouse corneal transplant rejection. (biomedsearch.com)
  • The aims of this study were to (1) evaluate a pachymetry technique to measure changes in mouse corneal thickness and (2) correlate increases in transplant thickness with clinical and histological features of rejection. (biomedsearch.com)
  • Graft transparency was graded and corneal thickness measured by pachymetry on alternate days. (biomedsearch.com)
  • Stromal cellularity increased with increasing corneal thickness up to approximately 300 microm. (biomedsearch.com)
  • PURPOSE: The purpose of this study was to evaluate outcomes of Descemet's stripping automated endothelial keratoplasty (DSAEK) using anterior stromal flawed (ASF) donor corneas that were unsuitable for use in full-thickness penetrating keratoplasty as a result of stromal scars, pterygia, or previous corneal refractive surgery and to compare results with DSAEK using standard tissue. (biomedsearch.com)
  • Central corneal thickness measurements should be performed before cutting to avoid tissue perforation. (biomedsearch.com)
  • ii) stroma: it makes up to 90% of the corneal thickness. (hindawi.com)
  • Thus, it has been hypothesized that the CXL therapy should have an inhomogeneous stiffening effect over full-corneal thickness. (healio.com)
  • Recent investigations of human corneal structure and biomechanics have shown that stromal collagen fibers (lamellae) are organized into a complex, highly intertwined three-dimensional meshwork of transverse oriented fibers that increases stromal stiffness and controls corneal shape. (arvojournals.org)
  • Corneal stroma is a very complex structure, composed of 200 lamellae of oriented collagen fibers. (metu.edu.tr)
  • In addition, linear deposits of amyloid fibrils are seen within the corneal stroma, disrupting the regular arrangement of corneal lamellae. (medscape.com)
  • Inside the stroma, collagen fibrils are organized into 1- to 2-μm thick sheet-like lamellae, which show a depth-dependent organization (ie, the anterior lamellae are interwoven while the posterior ones are arranged parallel to the surface). (healio.com)
  • Many diseases such as corneal dystrophies, scars or ectatic disorders induce a distortion of its anatomy or physiology leading to loss of transparency and subsequent loss of vision. (biomedcentral.com)
  • Recurrent corneal erosion (RCE) can develop after mechanical injury, as a complication of chronic ocular surface disease, or as a result of inherited dystrophies of the epithelial basement membrane or stroma. (bmj.com)
  • Corneal dystrophies. (medscape.com)
  • Three autosomal dominant corneal dystrophies map to chromosome 5q. (medscape.com)
  • Musch DC, Niziol LM, Stein JD, Kamyar RM, Sugar A. Prevalence of corneal dystrophies in the United States: estimates from claims data. (medscape.com)
  • Then HCEC sheets were transplanted onto the posterior stroma after descemetorhexis in the DSAEK group. (mdpi.com)
  • 3 The lattice-like arrangement of these collagen fibrils is necessary for corneal transparency. (arvojournals.org)
  • Multiple publications from different research groups are showing its potential benefits in relation to its capacity to improve or alleviate corneal scars, improve corneal transparency in metabolic diseases by enhancing the catabolism of the accumulated molecules, generate new organized collagen within the host stroma, and its immunosuppressive and immunomodulatory properties. (biomedcentral.com)
  • These changes result in loss of corneal transparency. (nih.gov)
  • 2012. Corneal myofibroblast biology and pathobiology: Generation, persistence, and transparency. (nih.gov)
  • These homeostatic characteristics are important for corneal transparency. (biomedcentral.com)
  • Keratoconus is being managed successfully with ultraviolet-A - riboflavin photochemical corneal cross-linking (known as 'CXL') throughout the world, which has revolutionized the field of corneal ectasia and related disorders. (portlandpress.com)
  • Daxer A, Fratzl P. "Collagen fibril orientation in the human corneal stroma and its implication in keratoconus. (wikipedia.org)
  • TEM revealed that TGFβ3 promotes mouse corneal stroma maturation by increasing stromal cell density and cell activates (more ER and Golgi), extracellular matrix formation/organization. (arvojournals.org)
  • The protein encoded by the Lum (lumican) gene, lumican, is a member of the small leucine-rich proteoglycan family, the predominant corneal keratan sulfate proteoglycan, is also found in interstitial extracellular matrices. (jax.org)
  • The stiffening effect of CXL therapy depends significantly on the composition and microstructure of corneal extracellular matrix. (healio.com)
  • Cellular therapy of the corneal stroma, with either ocular or extraocular stem cells, has been gaining a lot of interest over the last decade. (biomedcentral.com)
  • Patchy pigmentation was present in the corneal stroma and Descemet' membrane bilaterally. (hindawi.com)
  • Human corneal keratocyte cells cultured on laminin-mimetic peptide nanofibers retained their characteristic morphology, and their proliferation was enhanced compared with cells cultured on fibronectin-mimetic nanofibers. (jascoinc.com)
  • When these nanofibers were used for damaged rabbit corneas, laminin-mimetic peptide nanofibers increased keratocyte migration and supported stroma regeneration. (jascoinc.com)
  • A novel computerized approach to detection and counting of keratocyte cells from ultra high resolution optical coherence tomography (UHR-OCT) images of the human corneal stroma is presented. (uwaterloo.ca)
  • Ki67 positivity was detected in the upper stroma on day 1, 4, 7, and 14, and keratocyte apoptosis on day 1, 4, 7, and 14 after PRK, but not at an earlier or later time. (elsevier.com)
  • The frequency and spatial distribution of keratocyte proliferation and apoptosis are likely to be important determinants of the corneal wound healing process, but the detailed regulatory mechanisms have not yet been characterized. (elsevier.com)
  • Considering existing scientific evidence, it seems that all types of MSCs have similar behavior in vivo [Table 1 ], and thus are able to achieve keratocyte differentiation and modulate the corneal stroma with immunomodulatory properties [ 17 ]. (biomedcentral.com)
  • 2006. The keratocyte: Corneal stromal cell with variable repair phenotypes. (nih.gov)
  • Using a murine model of Aspergillus corneal infection, we also demonstrate that in the absence of RodA, A. fumigatus induces Dectin-1- and Dectin-2-dependent neutrophil recruitment to the corneal stroma and enhanced fungal killing. (jimmunol.org)
  • Collagen type I and type V are present in the same fibril in the avian corneal stroma. (rupress.org)
  • Homozygotes exhibit hyperextensible (8-fold reduced tensile strength) skin with abnormal collagen fibril structure and develop corneal opacity. (jax.org)
  • Lumican regulates collagen fibril assembly: skin fragility and corneal opacity in the absence of lumican. (jax.org)
  • 2018. Cell regulation of collagen fibril macrostructure during corneal morphogenesis . (cardiff.ac.uk)
  • 2018. Evolution of the vertebrate corneal stroma . (cardiff.ac.uk)
  • A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. (mdpi.com)
  • Near the corneal limbus, stromal cells expressed ABCG2, a protein present in many adult stem cells. (mendeley.com)
  • Furthermore, CXCR2 −/− mice developed only mild corneal opacification compared with BALB/c mice. (jimmunol.org)
  • characterized by opacification of corneal stroma. (uiowa.edu)
  • an autosomal dominant opacification of the central corneal stroma by needle-shaped polychromatic crystals. (drugs.com)
  • Descemet's stripping automated endothelial keratoplasty (DSAEK) using corneal donor tissue not acceptable for use in penetrating keratoplasty as a result of anterior stromal scars, pterygia, and previous corneal refractive surgical procedures. (biomedsearch.com)
  • Descemetoceles occur when the ulcer extends through the stroma, exposing Descemet's membrane . (wikipedia.org)
  • Highly reflective punctiform deposits starting from anterior to mid-stroma and increasing through Descemet's membrane were evident. (hindawi.com)
  • Corneal endothelial cells (CECs) are firmly attached to the underlying Descemet's membrane. (mdpi.com)
  • Kynurenine (KYN) is a metabolite of tryptophan, proposed for the treatment of corneal diseases. (bioportfolio.com)
  • Corneal ulcers are one of the most common eye diseases in dogs. (wikipedia.org)
  • A study of congenital eye disease at a teaching hospital in Sagamu, Nigeria, found that corneal opacities is the fourth most common congenital eye disorder causing blindness in 5.7% of infants with congenital eye diseases (Bodunde et al, 2006). (aao.org)
  • As a result, it will improve our understanding of corneal biology and pathobiology and foster development of new treatment regimens for diseases causing corneal blindness. (grc.org)
  • Scattering increases with age in cuartic manner, 4 but elevated scattering can also be the result of specific ocular diseases, such as corneal edemas and swelling 7 , 8 or cataracts affecting the crystalline lens. (spiedigitallibrary.org)
  • Corneal topography, pachymetry, and manifest astigmatism were not significantly different between groups. (biomedsearch.com)
  • Corneal topography with central uniform steepening following hyperopic LASIK. (medscape.com)
  • CXCR2 −/− and CCR1 −/− mice were immunized s.c. and injected into the corneal stroma with Ags from the parasitic helminth Onchocerca volvulus . (jimmunol.org)
  • These differences were not due to impaired KC and MIP-2 production in the corneal stroma of CXCR2 −/− mice, which was similar to BALB/c mice. (jimmunol.org)
  • Corneal Toxicity of Topical Tacrolimus Ointment in Mice with Corneal Epithelial Injury. (bioportfolio.com)
  • Mice with a 1-Mb duplication developed corneal hyperplasia and thymic tumors, while two different 3- to 4-cM deficiencies were embryonically lethal in heterozygous mice. (genetics.org)
  • METHODS: Orthotopic corneal allografts (C57BL/6 strain donor) and syngeneic grafts were performed in A/J mice. (biomedsearch.com)
  • We investigated the role of semaphorin (SEMA) 3C in corneal wound healing and reinnervation in normal and diabetic B6 mice. (diabetesjournals.org)
  • 2007. Ultraviolet radiation-induced corneal degeneration in 129 mice. (nih.gov)
  • Our objective was to identify whether depletion of Pax6 in adult mice caused any corneal abnormalities, similar to those in Pax6 + / − mice, where Pax6 levels are low throughout development and adulthood. (springer.com)
  • It is not known if all corneal abnormalities in adult Pax6 + / − mice are predetermined by abnormal development or whether some are caused by reduced Pax6 in the adult. (springer.com)
  • Our aim was to determine whether depletion of Pax6 in adult mice caused corneal abnormalities, comparable to those reported for Pax6 + / − mice. (springer.com)
  • As Aspergillus and Fusarium species are also major causes of corneal infection and blindness worldwide ( 6 ), the current study examined the role of hydrophobins in a murine model of fungal keratitis ( 7 , 8 ). (jimmunol.org)
  • Corneal disease leading to vision loss is a major cause of blindness worldwide and a World Health Organization priority disease, particularly in the developing world [ 1 ]. (mdpi.com)
  • Despite extensiveresearch on corneal wound healing, corneal scarring remains a major cause of preventable blindness. (diva-portal.org)
  • Further analysis of these cells will aid elucidation of molecular mechanisms of corneal development, differentiation, and wound healing. (mendeley.com)
  • There has been no effective treatment or agent that is available for corneal injury in promoting corneal wound healing. (biomedcentral.com)
  • This could be a novel breakthrough as both cell proliferation and functional maintenance are important during corneal wound healing. (biomedcentral.com)
  • The process of corneal wound healing is complex. (diva-portal.org)
  • Shortly, keratocytesadjacent to the corneal wound undergo apoptosis and remaining cells start the process of proliferation andmigration in order to close the wound. (diva-portal.org)
  • However, when the process of corneal wound healing is incompleteor disturbed, corneal scarring may occur, which can lead to significantly impaired vision. (diva-portal.org)
  • The Effects of Distance Between the Corneal Apex and the Inner and Outer Corneal Wound Edges on Corneal Astigmatism After Cataract Surgery. (tripdatabase.com)
  • Common eye conditions that can cause eye pain are conjunctivitis, corneal abrasion, and hordeolum, and some of the most serious eye conditions include acute angle-closure glaucoma, orbital cellulitis, and herpetic keratitis. (aafp.org)
  • METHODS The records were reviewed of patients who had previously been examined and treated for recurrent corneal epithelial erosion and who presented again with signs suggestive of a microbial keratitis. (bmj.com)
  • Eleven patients were identified who had previously been treated for RCE and who presented again with a corneal infiltrate suggestive of a microbial keratitis. (bmj.com)
  • The role of α-toxin in corneal infection (keratitis) and infection of the interior of the eye (endophthalmitis) has been well established by comparing virulence in animal models and α-toxin-deficient isogenic mutants with their wild-type parental strains. (nih.gov)
  • Cell density distribution analysis was carried in 3D UHR-OCT images of the human corneal stroma, acquired in-vivo. (uwaterloo.ca)
  • Cellular delivery into the corneal stroma has been experimentally assayed in vivo in multiple ways: systemic versus local injections with or without a carrier. (biomedcentral.com)
  • This observation encouraged earlier research by the authors to assess the variation of corneal stiffness with age using tests on ex vivo donor corneas (Elsheikh et al . (royalsocietypublishing.org)
  • In order to meet the high demand for transplants with an appropriate human corneal endothelial cell density as a prerequisite for corneal function, several tissue engineering techniques have been developed to generate transplantable endothelial cell sheets. (mdpi.com)
  • A variety of refractive surgery techniques, which reshape the corneal stroma using laser energy, have been marketed as simple and safe alternatives to glasses or contact lenses. (aafp.org)
  • We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. (nih.gov)
  • When corneal fibrosis is considered secondary to inflammation, it should not be diagnosed separately unless warranted by its severity, though it should be described in the pathology narrative. (nih.gov)
  • Animal studies suggest that these acute injuries can be followed by recurrent or delayed symptoms, including ulceration, inflammation, and corneal erosion. (nap.edu)
  • Development of therapeutic methods to enhance corneal regeneration is essential for treatment of these defects. (jascoinc.com)
  • [ 5 ] Photorefractive keratectomy (PRK) has been used successfully for hyperopia but has problems of regression, induced astigmatism, and corneal haze, thereby limiting its usefulness to the correction of mild hyperopia only. (medscape.com)
  • the mean change in anterior, posterior, and total corneal astigmatism at 1 week and anterior astigmatism at 1 month were statistically significantly greater in the inner incision close group than in the inner incision far group. (tripdatabase.com)
  • To compare the effects of the distance from the inner and outer corneal incision wounds to the corneal apex on the changes in corneal astigmatism after cataract surgery.Seventy-two eyes with anterior corneal astigmatism less than 2.50 diopters (D) were evaluated. (tripdatabase.com)
  • This study aimed at investigating the proliferation and apoptosis of corneal cells following photorefractive keratectomy (PRK) treatment. (elsevier.com)
  • Cloned corneal SP cells under chondrogenic conditions produced matrix staining with toluidine blue and expressed cartilage-specific markers: collagen II, cartilage oligomatrix protein, and aggrecan. (mendeley.com)
  • These results demonstrate the presence of a population of cells in the human corneal stroma expressing stem cell markers and exhibiting multipotent differentiation potential. (mendeley.com)
  • These cells may be a resource for bioengineering of corneal stroma and for cell-based therapeutics. (mendeley.com)
  • the migration and differentiation of periocular mesenchymal cells of neural crest origin for the formation of ocular tissue stroma, 2. (grc.org)
  • Human corneal endothelial cells (HCECs) do not replicate after wounding. (mdpi.com)
  • Fluorescein microscopy one month after surgery showed numerous DiI-labelled cells on the posterior corneal surface in the DSAEK group. (mdpi.com)
  • Corneal endothelial cells are believed to be of neural crest origin [ 1 , 2 ] and form a monolayer of hexagonal cells. (mdpi.com)
  • In addition to well-recognized contraindications (e.g., unstable refraction, pregnancy and lactation, chronic eye disease, systemic illness, corneal abnormalities), there are other conditions that warrant caution (e.g., excessively dry eyes, contact lens intolerance, chronic pain syndromes). (aafp.org)
  • Photokeratoscope images revealed irregularity of the corneal surface at the sites of the opacities. (molvis.org)
  • Anterior segment optical coherence tomography (OCT) may be used to measure the depth of the corneal opacities. (medscape.com)
  • The prevalence of congenital corneal opacities (CCO) is estimated to be 3 in100,000 newborns. (aao.org)
  • Table 2 highlights the differential diagnosis of infantile corneal opacities and the method of diagnosis. (aao.org)
  • As the keratis area takes on a whiter appearance, the area of corneal involvement resembles the sclera more closely. (uiowa.edu)
  • The present study examines the relationship between dose to corneal substructures and incidence of corneal toxicity within six months of proton beam therapy (PBT) for uveal melanoma. (bioportfolio.com)
  • Corneal cross-linking (CXL) is a relatively new treatment that is currently used to halt the progression of this eye disease. (healio.com)
  • AIMS To describe the clinical features of patients with a history of recurrent corneal epithelial erosion who develop acute corneal infiltration. (bmj.com)
  • CONCLUSIONS Corneal infiltrates are an uncommon complication of recurrent corneal epithelial erosion. (bmj.com)
  • Called also superficial corneal erosion syndrome, Boxer ulcer. (thefreedictionary.com)
  • In this review, we examine elastic tissue in the corneal stroma with some mention of other ocular structures which have been more thoroughly described in the past. (elsevier.com)
  • These equations, which closely matched the experimental results, depicted corneal stiffening with age in a form suitable for implementation in numerical simulations of ocular biomechanical behaviour. (royalsocietypublishing.org)
  • There have been at least two cases where corneal ulceration was caused by canine herpesvirus . (wikipedia.org)
  • The range of fiber angles and the fiber percentage having an angular displacement greater than ±3.5° relative to the corneal surface ("transverse fibers") was highest in the anterior stroma and decreased with depth. (arvojournals.org)
  • The lack of a preferred distribution of angled fibers with regard to corneal quadrant or radial position likely serves to evenly distribute loads and to avoid the formation of areas of stress concentration. (arvojournals.org)
  • 1 Corneal shape, which crucially defines its refractive function, is thought to be controlled by the mechanical properties of these larger collagen fibers and their three-dimensional (3-D) structural organization. (arvojournals.org)
  • Epithelial debridement wounds result in the loss of nerve endings and retraction of stroma nerve fibers. (diabetesjournals.org)
  • To investigate the stiffening effects of corneal cross-linking (CXL) on tensile properties of anterior and posterior corneal flaps. (healio.com)
  • An explanation of this new technology, consisting of optical coherence tomography integrated to the microscope in both anterior and posterior lamellar corneal transplants (DALK, DSAEK, DMEK). (intechopen.com)